References of "Poster"
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See detailEtude de la production, la lyophilisation et la conservation d’un starter lactique du lben : lait traditionnel fermenté.
Ziada, M.; Touhami, Y.; Achour, M. et al

Poster (2006, November)

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See detailL'affirmation de soi par la pratique de l'acteur : évaluation d'un groupe thérapeutique
Delvaux, Muriel ULg; Roodthooft, Dominique; Sarto, Danielle et al

Poster (2006, November)

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de ... [more ▼]

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de techniques théâtrales à un programme de thérapies comportementales et cognitives « classique ». En expérimentant la pratique de l’acteur, ce groupe visait à permettre aux participants de s’entraîner à se mettre en éveil, d’élargir leurs champs de perceptions et de compréhensions, de prendre conscience du langage du corps en dehors de la phrase verbale, de rechercher les mécanismes qui empêchent de s’affirmer, et de mettre en place des compétences qui améliorent l’affirmation de soi et augmentent ainsi les possibilités d’action. [less ▲]

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See detailLactation effect on stable isotope ratios and mercury levels in the blood of northern elephant seals
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Poster (2006, October 27)

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios ... [more ▼]

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on day 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1 ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. To conclude, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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See detailAnálisis filogenético de aislamientos argentinos del virus de la leucosis bovina
Rodriguez, Sabrina ULg; Trono, K.; Jones, L.R.

Poster (2006, October 22)

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un ... [more ▼]

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un retrovirus oncogénico B-linfocitotrópico que, junto con los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus, HTLV) y su contraparte en los simios (Simian T-Lymhotropic Virus, STLV), están incluidos en el género Deltaretrovirus dentro de la familia Retroviridae. BLV es un retrovirus ampliamente distribuido entre la población bovina a nivel mundial y es el agente etiológico de la leucosis bovina enzoótica (LBE), una enfermedad crónica linfoproliferativa de ocurrencia natural en el ganado bovino que constituye la enfermedad neoplásica más frecuente del ganado bovino lechero. El estudio de la diversidad genética y la comparación de aislamientos de BLV de diferentes orígenes ha sido abordado por otros autores. Sin embargo, poco se conoce acerca de la diversidad genética en aislamientos argentinos de BLV (Vet. Microbiol. 96(1):17-23; Arch.Virol.150(3):443-458). El gen env ha sido utilizado en el estudio de relaciones filogenéticas para este virus. Una gran cantidad de secuencias de este gen, correspondientes a aislamientos de BLV de distintas áreas del mundo, se encuentran disponibles en las bases de datos públicas. OBJETIVOS Estudiar la diversidad genética y las relaciones filogenéticas de los aislamientos argentinos de BLV en relación con aislamientos de otras regiones del mundo. MATERIALES Y METODOS Se utilizaron muestras clínicas provenientes de 28 bovinos adultos naturalmente infectados con BLV provenientes de distintas zonas geográficas del país como material de partida. Se diseñaron primers y se optimizaron las condiciones de una Reacción en Cadena de la Polimerasa (PCR) para la amplificación de la totalidad de la región genómica codificante para el gen env de provirus de BLV. La extracción del DNA genómico proviral se realizó a partir de tejido tumoral y de sangre entera en los casos de animales infectados sin evidencias clínicas. Los productos de PCR obtenidos fueron purificados y secuenciados directamente. La matriz de datos fue construida incluyendo las secuencias de BLV reportadas en este trabajo y secuencias correspondientes a cepas representativas de diversas regiones geográficas. Se incorporaron en el análisis secuencias homólogas de virus relacionados, las cuales fueron utilizadas como outgroup. Las secuencias fueron alineadas mediante el programa Muscle y analizadas mediante las rutinas de Parsimonia del programa TNT. Con el fin de evaluar el soporte de los grupos identificados se llevaron a cabo análisis de Bootstrap y Jackknife. CONCLUSIONES El estudio de caracterización molecular reveló una relativa estabilidad genética para los aislamientos argentinos de BLV, apoyando el hecho de que la variación genética de estos virus complejos del grupo es menor que la de otros géneros de la familia de los retrovirus. Por otra parte, del análisis filogenético se desprende el hecho de la existencia de un agrupamiento de aislamientos representativos de la región que conforman un clado único. No se observaron clados exclusivos de regiones geográficas dentro de Argentina. [less ▲]

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See detailInmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Gutiérrez, G.; Vissani, A. et al

Poster (2006, October 22)

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See detailEffect of SK channel blockade on the firing of dorsal raphe neurons in anaesthetized rats
Alleva, Livia ULg; Rouchet, Nathalie; Waroux, Olivier ULg et al

Poster (2006, October 17)

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential ... [more ▼]

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential, and therefore is important in the regulation of the firing frequency and/or pattern of many types of neurons. Serotonergic (5-HT) neurons from the raphe nuclei express SK channels and exhibit a significant AHP which can be efficiently blocked in vitro by apamin and N-methyl laudanosine (NML) (Scuvée-Moreau et al, 2004). In the later study, we found that some but not all neurons (50%) had a significantly increase in their firing rate when positive current was injected after SK channel blockade. In order to determine the physiological relevance of these channels in vivo, single unit extracellular recordings were carried out in anesthetized rats and combined with iontophoresis of the specific non-peptidic SK channel blocker, UCL1684. 5-HT neurons were tested for their inhibitory response to locally applied 5-HT and histological analysis confirmed the localization of the recording site. UCL 1684 was used at a concentration of 200 µM. Out of 11 neurons recorded, 6 showed a significant increase in the production of doublets, with no effect on their mean firing rate as compared to the control condition. The other neurons were completely unaffected. These results suggest that the responsiveness of presumed 5-HT neurons to SK channel block is variable. Although the use of 200 µM UCL allow us to be sure of a sufficient SK blockade at the recording site (Waroux et al, 2005), we can not rule out the possibility that SK channels present at the dendritic level were not completely blocked. In conclusion, SK channels in vivo might play a role in controlling the firing pattern of a subgroup of 5-HT neurons. [less ▲]

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See detailIncorporation in bread of dietary fibres from by-products of the agro-transformation.
Roiseux, Olivier; Blecker, Christophe ULg; Sindic, Marianne ULg et al

Poster (2006, October 17)

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See detailStudy of the physical changes of spray-dried inulin during storage.
Ronkart, Sébastien; Paquot, Michel ULg; Fougnies, C. et al

Poster (2006, October 17)

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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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See detailDiet of Damselfishes (Pomacentridae): a multidisciplinary approach
Fabri, Grégory ULg; Parmentier, Eric ULg; Lepoint, Gilles ULg et al

Poster (2006, October)

Coral reefs are the marine ecosystem showing the greatest fish diversity. Damselfishes (Pomacentridae) represent, with more than 340 species, one of the most important families in the coral reef ... [more ▼]

Coral reefs are the marine ecosystem showing the greatest fish diversity. Damselfishes (Pomacentridae) represent, with more than 340 species, one of the most important families in the coral reef environment. Currently their diet is understudied. This work has 2 aims: (i) to characterize the diet of 13 pomacentrid species of the reef of Toliara (Madagascar) and (ii) to investigate if the specific diversity of this family would result from a strong trophic segregation. A multidisciplinary approach including morphological data (teeth, lower jaw-lever mechanics and intestine length), stomach contents and stable isotope analysis were used. The morphological approach and the stomach contents show that each studied species is able to capture small planktonic preys (e.g. copepods). However, the 13 species can be divided into two trophic guilds: alguivores and planktivores (respectively species where the filamentous algae and the planktonic preys count for more than 60% of their diet). Within these two principal classes, the analysis of the stomach contents and stable isotopes permit to define sub-groups : (1) the species having a food behaviour exclusively alguivore or planktivore (> 90% of their diet) (2) species showing a more varied diet by also eating other types of preys such as vagiles and/or sessiles invertebrates. The diet would contribute but could not explain all diversity in Pomacentridae. Some species show a very similar diet. Consequently other ecological factors should be responsible for the reduction of interspecific competitions and for the existence of such diversity. [less ▲]

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See detailMelting and polymorphic behavior of binary blends made of palm oil or its fractions and other fats.
Danthine, Sabine ULg; deroanne, claude

Poster (2006, October)

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See detailXylanase production by Penicillium canescens in solid-state fermentation.
Assamoi, A.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2006, October)

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See detailStudy of the effect of exposition of MCF-7/bos cells to 17-ß-estradiol by 2D DIGE
Collodoro, Mike; Dobson, Rowan ULg; Bertrand, Virginie ULg et al

Poster (2006, October)

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that ... [more ▼]

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that will enable the detection (and eventual quantification but not identification) of numerous compounds having an estrogenic activity in a single rapid test. [less ▲]

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See detailIncorporation in bread of dietary fibres from by-products of the agro-transformations.
Roiseux, Olivier; Blecker, Christophe ULg; Vandebeke, E. et al

Poster (2006, October)

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See detailDistribution of nerve fibres in bovine and human mucosal associated lymphoid tissues
Defaweux, Valérie ULg; Dorban, G.; Antoine, Nadine ULg et al

Poster (2006, October)

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD ... [more ▼]

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD, but little, if any, in sCJD or BSE. In particular, the BSE strain is associated with significant PrP-res accumulation in tonsils, spleen and appendix in humans, whereas it is largely confined to the nervous system in infected cattle. Therefore, at least in the case of BSE and vCJD, it appears that host properties can influence the accumulation of the infectious agent in lymphoid organs. Mature FDC play an important role in prion pathogenesis, since neuroinvasion following peripheral challenge is significantly impaired in their absence. The proximity between these FDC and sympathetic nerve endings is known to affect the speed of prion neuroinvasion. In this study, we analysed the mucosal innervation and the interface between nerve fibres and FDC in bovine and human tonsils and in ileal and jejunal bovine Peyer’s patches using a panel of antibodies observed by confocal microscopy. Since differences in the innervation of lymphoid organs depending on age have been reported, we analysed three categories of bovine ages (new born calves, calves less than 12 months old and bovines older than 24 months) and two categories of human ages (patients less than 5 years old and patients older than 25 years). In both species, hypothetical ways of innervation by-passing germinal centre could be postulated: nerve fibres are widely distributed in antigens/cells traffic area (the lamina propria, the interfollicular zone, the suprafollicular dome in Peyer’s patches and the lymphoepithelial area in tonsils). We pointed out that, only in ileal and jejunal Peyer’s patches and in tonsils of bovines older than 24 months, nerve fibres are observed to be in contact with FDC. In contrast, in human tonsils, no nerve fibres established contact with FDC, whatever the age. Thus, innervation of germinal centres can be said to be an age-dependent dynamic process in bovines and a weak innervation of the secondary lymphoid organs could thus be a rate-limiting step to neuroinvasion in humans. This variation could influence the way of neuroinvasion and thus, the differences of susceptibility of bovines and humans to the BSE agent. [less ▲]

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See detailPhysicochemical characterization of a dietary fibre concentrate extracted from date pulp.
Roiseux, Olivier; Blecker, Christophe ULg; Besbes, Souhail et al

Poster (2006, October)

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See detailLa décomposition morphologique des mots et des pseudo-mots chez les apprentis lecteurs et les dyslexiques
Quemart, Pauline ULg; Casalis, Séverine; Mathiot, Emmanuelle

Poster (2006, October)

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See detailAre CNA42 and FDC-B1 directed against ovine follicular dendritic cells?
Toppets, Vinciane ULg; Piret,J; Minne , M et al

Poster (2006, October)

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See detailNew Cold Plasma and DLC Surface Treatments for Medical Co-Cr-Mo and Ti-6Al-4V Prostheses
Matarazzo, Lucas; Gillet, Marie-Claire ULg; Forest, B et al

Poster (2006, September 27)

Recent studies indicate that the most important factor limiting the longevity of hip prostheses is the production of wear particles from the prosthetic components. We have studied the effect of two ... [more ▼]

Recent studies indicate that the most important factor limiting the longevity of hip prostheses is the production of wear particles from the prosthetic components. We have studied the effect of two surface treatment techniques on the Ti-6Al-4V alloy: ionic nitruration (cold plasma) and Diamond-like Coating (DLC). These two techniques create a layer of 7-8 μm in depth on the surface of the alloy, modifying its structure and hardening it considerably. Biocompatibility was analyzed using cellular culture to compare viability, proliferation (DNA quantification) and differentiation (ALP assay) of human osteoblast-like cells (MG 63) as well as cellular adhesion of human fibroblasts (WI 38) or osteoblasts, under SEM. [less ▲]

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See detailConstruction d’un modèle du récepteur sérotoninergique 5HT2c
Dilly, Sébastien ULg; Farce, Amaury; Yous, Said et al

Poster (2006, September 27)

La 5-hydroxytryptamine, plus couramment appelée sérotonine, a été découverte indépendamment en 1937 dans le tractus gastro-intestinal, et en 1948 dans le sérum, puis trouvée également dans le cerveau en ... [more ▼]

La 5-hydroxytryptamine, plus couramment appelée sérotonine, a été découverte indépendamment en 1937 dans le tractus gastro-intestinal, et en 1948 dans le sérum, puis trouvée également dans le cerveau en 1953. Sa structure a pour sa part été déterminée en 1949. Cette découverte a été initialement basée sur ses propriétés contractantes sur les muscles lisses. Le système sérotoninergique est cependant beaucoup plus important que ces premières investigations ne le laissaient envisager, puisqu’il intervient pratiquement dans toutes les réponses physiologiques, tant au niveau périphérique, où la sérotonine est impliquée dans l’hypertension, l’asthme ou l’acidité gastrique, qu’au niveau central, où elle joue un rôle majeur dans la régulation de l’humeur. Cette capacité lui donne une grande importance dans le traitement des dépressions. C’est dans ce cadre que se placent nos travaux, portant plus particulièrement sur une nouvelle classe thérapeutique dont le chef de file est l’agomélatine. Cette dernière agit selon un mode d’action original en se fixant à la fois aux récepteurs mélatoninergiques et au récepteur sérotoninergique 5HT2c, ce qui a donné naissance au concept MASSA (Melatonin Agonist and Selective Serotonin Antagonist). Comme la plupart des récepteurs sérotoninergiques, le sous-type 5HT2c est un Récepteur à sept domaines transmembranaires Couplé à une Protéine G (RCPG). La structure tridimensionnelle de 5HT2c n’est pas connue expérimentalement, et nous avons donc fait appel à la modélisation par homologie comparative pour construire un modèle nous permettant d’étudier ses interactions avec l’agomélatine. [less ▲]

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See detailBiocompatibilidad de nuevos policationes sintéticos sobre las propiedades mecánicas y morfológicas de la membrana eritrocitaria
Relancio, M; Delannoy, M; Foresto, P et al

Poster (2006, September 25)

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See detailEvaluación mediante métodos ópticos de la funcionalidad de policationes sintéticos para el enmascaramiento de sitios antigénicos en el eritrocito humano
Relancio, M.F; Riquelme, B; Dumas, D et al

Poster (2006, September 25)

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See detailUtilization of lupin seed as the main protein source in broiler chicken feeding : influence of the variety and interest of protease addition
Froidmont, Eric; Beckers, Yves ULg; Dehareng, Frédéric et al

Poster (2006, September 18)

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See detailIgE controlling loci in Czech atopic patients.
Gusareva, Elena ULg; Havelková, Helena; Blažková, Hanna et al

Poster (2006, September 07)

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See detailEffects of synthetic polycations on erythrocyte membrane analyzed by optical methods
Relancio, F; Riquelme, B; Dumas, D et al

Poster (2006, September 02)

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See detailFormulation de la lipase de Yarrowia lipolytica
Alloue Waze, M.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2006, September)

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See detailOptimized conditions for the preparation of Pt supported catalysts by deposition-precipitation
Cellier Caroline; Lambert, Stéphanie ULg; Ruaux Vincent et al

Poster (2006, September)

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See detailSelection of bacteriocin-producing lactic acid bacteria from Senegalese traditional fermented food for use as bio-preservative in fish
Diop, M.; Dortu, C.; Ngom, A. et al

Poster (2006, September)

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See detailProduction du gari ordinaire par inoculation de cultures de Lactobacillus plantarum
Egounlety, M.; Adjakidjé, A.; Yao, A. et al

Poster (2006, September)

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See detailImmobilisation de la lipase de Yarrowia lipolytica.
Kabran, P.; Alloue, M.; Destain, Jacqueline ULg et al

Poster (2006, September)

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See detailCharacterisation of bacteriocins from lactic acid bacteria
Dortu, C.; Franz, C.; Kostinek, M. et al

Poster (2006, September)

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See detailAssessment of a bone biopsy technique for tiludronic acid dosage in horses: a preliminary study
Delguste, Catherine ULg; Doucet, Michèle; Gabriel, Annick ULg et al

Poster (2006, September)

Introduction Tiludronic acid as a bisphosphonate has a strong affinity for bone, making difficult the assessment of its PK profile in this deep compartment on living animals. The invasive nature of sample ... [more ▼]

Introduction Tiludronic acid as a bisphosphonate has a strong affinity for bone, making difficult the assessment of its PK profile in this deep compartment on living animals. The invasive nature of sample collection remains a limiting factor. This study was carried out in order to assess a bone biopsy technique allowing the repetition of sampling over a long period of time to dose tiludronic acid in equine bone. Material and Methods Six healthy 4- to 8-year-old Standardbred geldings were treated with tiludronic acid 1 mg/kg in a saline infusion over 30 minutes. The horses were subjected to euthanasia on days 1, 43, 57, 92, 182 and 222 post-treatment, respectively. Bone samples (test samples and larger reference samples) were taken at 4 sites per side and per horse: the lateral aspect of the metacarpal bone III (MCIII) of the forelimb, the 13th rib, the tuber coxae and the cuboïd bone. Test samples were taken with a 5-mm diameter dental drill (Implanteo™, Anthogyr), while larger reference samples were taken around the drill sample sites with an osteotome. All samples were taken immediately after euthanasia. Tiludronic acid concentrations were measured by HPLC with UV detection. Results The tuber coxae was the easiest site to sample. The sample site of the MCIII was easily accessible but due to the extreme hardness of the bone, the drill sampling was technically difficult to perform. Drill samples obtained from the 13th rib were very small, and the access more limited. Finally, the access to the cuboïd bone required considerable dissection, not performable in vivo. Extraction and dosage of tiludronic acid from the MCIII was difficult for technical reasons in most cases, in drill samples as well as in reference samples, and most (96%) of the values obtained were considered unreliable. This was also true for some samples from the 13th rib and from the cuboïd bone, to a lesser extent (42% of unreliable values for both sites). Moreover, for these two sample sites, less technical problems were encountered for the extraction and dosage of tiludronic acid in drill samples than in reference samples. No extraction or dosage problem was encountered with the tuber coxae samples. The ratio of tiludronic acid concentrations in drill versus reference samples ranged from 73% to 185% (mean: 124%), 65% to 208% (mean: 118%), and 26% to 110% (mean: 62%) respectively in the tuber coxae, 13th rib and cuboïd bone. In all but one horse, the highest concentrations in tiludronic acid were found in the tuber coxae, while the lowest values tended to be in the cuboïd bone at any time post-treatment. Tiludronic acid was still found in all bone samples 7 months after treatment. Discussion As previously reported with other tools, the drill tested in this study should permit to conveniently perform bone biopsies in the equine tuber coxae. This biopsy site would be the most appropriate for repeated sampling to dose tiludronic acid over time. This would help to design appropriate PK/PD studies with tiludronic acid in horses. This study also further confirms the long persistence of tiludronic acid in equine bone. [less ▲]

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See detailImprovement of autobiographical memory in schizophrenia patients
Blairy, Sylvie ULg; Neumann, Aurore; Pierret, Leslie et al

Poster (2006, September)

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See detailFixed implant rehabilitation of the edentulous maxilla: a systematic literature review.
LAMBERT, France ULg; Weber, HP; Gallucci, German et al

Poster (2006, September)

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See detailDendritic cells: potential actors in prion neuroinvasion.
Dorban, G.; Lallemand, C.; Defaweux, Valérie ULg et al

Poster (2006, September)

Detailed reference viewed: 11 (7 ULg)
See detailSelection of bacteriocin-producing lactic acid bacteria from Senegal traditional fermented food
Diop, M.; Dubois Dauphin, Robin ULg; Tine, E. et al

Poster (2006, September)

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See detailPrenatal development study of rat corti organ at the morphological level
Thelen, Nicolas ULg; Malgrange, Brigitte ULg; Thiry, Marc ULg

Poster (2006, September)

Although the structural organization of the mature Corti organ is generally well defined, little is known about its prenatal development. Here, we have examined by photonic and electron transmission ... [more ▼]

Although the structural organization of the mature Corti organ is generally well defined, little is known about its prenatal development. Here, we have examined by photonic and electron transmission microscopies the morphological changes occurring in the cochlear epithelium from the embryonic day 16 to 19 in rat. Moreover, we have used the periodic acid-thiocarbohydrazide-silver proteinate method to detect the glycogen particles on semithin and ultrathin sections of the cochlear duct. <br />At the embryonic day 16 (E16), whatever the region of the cochlear studied (base, medium, apex), the organ of Corti is not present. The cochlear epithelium appears as a pseudostratified epithelium formed formed by two distinct regions according to the presence or the absence of microvilli at the apical surface of cells. The region with the microvilli is on the modiolar side and the presence of microvilli is a characteristic of cells in the greater epithelial ridge (GER). In basal part of the cochlear duct, glycogen particles are visible on ultrathin sections in only one cell. On both sides of this cell, apical part of the epithelium shows microvilli. At E17, on semithin sections of basal and medium parts of cochlear duct, one cell visible on the whole height of the epithelium shows rich content in glycogen . At E18, in the majority of sections realized at the base and medium of the cochlear canal, the organ of Corti is relatively well recognizable. We distinguish principally the inner hair cells but also to a lesser extent the outer hair cells as well as the supporting cells (pillar, phalengeal and Deiters cells).We show that only the pillar cells have a rich content in glycogen. At E19, the organ of Corti is clearly recognizable in all the basal and medium parts of the cochlear duct. By contrast, it is yet differenciated in the apex. <br />These data seem to indicate that the Corti organ in rat develops from base to apex in the greater epithelial ridge starting by the pillar cells. [less ▲]

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See detailDeveloping innovatie structures and mechanisms aiming to enhance the transfert of technologies from public institutions to the private sector.
Lardinois, M.; Totte, A.; Tounkara, L. et al

Poster (2006, September)

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See detailLe NPI NH chez des sujets âgés déments et non déments
Ylieff, Michel ULg; Di Notte, David; Missotten, Pierre ULg et al

Poster (2006, September)

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See detailVirus-like Particles of Human and Bovine Noroviruses
Mauroy, Axel ULg; Scipioni, Alexandra; Mast, Jan et al

Poster (2006, September)

1.Introduction and Objectives Noroviruses have a positive-sense, single-stranded RNA genome of about 7.5 kb containing three open reading frames (ORFs). ORF1 encodes the non-structural proteins, ORF2 ... [more ▼]

1.Introduction and Objectives Noroviruses have a positive-sense, single-stranded RNA genome of about 7.5 kb containing three open reading frames (ORFs). ORF1 encodes the non-structural proteins, ORF2 encodes the single capsid protein with a molecular weight of 56 kD and ORF3 encodes a minor structural protein. These viruses are very stable and successful infection can be achieved even with very low infectious dose. Human noroviruses are the major cause of non bacterial, food-borne gastroenteritis worldwide. Contamination is usually oro-fecal (Green et al. 2001). Belonging to the Caliciviridae family, the genus Norovirus also contains viruses infecting animals, in particular bovines. This genus is divided into five genogroups (Ando et al. 2000). The existence of bovine noroviruses raises the question of their possible zoonotic character; the viruses could be transmitted to human by contaminated effluents for example (Scipioni et al., this congress). The study of norovirus is hampered by the lack of a cell culture system. However virus like particles (VLPs) can be produced and they are antigenically and morphologically similar to the wild virus (Jiang et al. 1992). The aim of this work was to obtain VLPs from human and bovine strains. Such VLPs will be used as antigens to produce specific polyclonal and monoclonal antibodies. 2.Material and Methods The capsid protein genes of a human norovirus strain (H384) and a bovine strain (B309) were amplified by RT PCR from stools collected from human and veterinary diagnostic laboratories. These sequences were analysed by the Basic Local Alignment Search Tool (BLAST) available on the NCBI website. Plasmids containing the ORF2 sequence of two reference human strains, the Norwalk (NV, belonging to the genogroup I) and the Hawaii (HV, belonging to the genogroup II) strains, were kindly received from Jan Vinje (University of North Carolina, USA). The baculovirus protein expression system (Invitrogen®) was used to obtain VLPs. Briefly, cells and supernatants were harvested after freezing/thawing cycles. Cells were pelleted. VLPs were purified from supernatants by ultracentrifugation through a 30% sucrose cushion and then by isopycnic ultracentrifugation in CsCl. Fractions resulting from the gradient were analysed by SDS-PAGE. Protein assembly into VLPs was checked by electron microscopy. 3.Results H384 strain ORF2 is closely related to the HuNV/Altenkirchen 140/01/DE strain (99% of identity). Thus, H384 most likely belongs to genogroup II genotype 4 (GII.4, the Lordsdale group). B309 capsid gene is closely related to genogroup III genotype 2 (Newbury2). Electron microscopy confirmed the presence of norovirus-like particles in the supernatant of recombinant baculovirus infected cell culture. By SDS PAGE applied on the fractions resulting of the CsCl gradient, a protein of 56 kD was detected and confirmed the expression of the capsid protein. Therefore, VLPs were obtained for all the 4 strains investigated. 4.Discussion and Conclusions Currently we dispose of representative surrogates of three norovirus genogroups (GI, GII and GIII). H384 (GII.4) is a representative of the most commonly diagnosed genotype from current outbreaks in many countries (Blanton et al. 2006). These VLPs will therefore serve as antigens to raise polyclonal and monoclonal antibodies. These tools will be used for the diagnosis of the related infections in human and domestic animals. [less ▲]

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See detailChemical composition and lipid fraction characteristics of aleppo pine (Pinus halepensis Mill.) seeds cultivated in Tunisia
Cheikh-Rouhou, S.; Besbes, S.; Hentati, B. et al

Poster (2006, September)

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See detailEvaluation du stade évolutif de la démence par la Clinical Dementia Rating-Modified (CDR-M)
Ylieff, Michel ULg; Di Notte, David; Missotten, Pierre ULg et al

Poster (2006, September)

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See detailTime-resolved SAXS study of the formation of resorcinol-formaldehyde aqueous gels
Job, Nathalie ULg; Gommes, Cédric ULg; Goderis, B. et al

Poster (2006, August 06)

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See detailA non-traumatic case of intracranial haemorrhage in a horse
Verwilghen, Denis ULg; Van Galen, Gaby ULg; Peters, Fabrice et al

Poster (2006, August)

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See detailA case of periarticular abcess and suppurative arthritis of the atlanto-occipital joint
Van Galen, Gaby ULg; Lopez, Diego; Sandersen, Charlotte ULg et al

Poster (2006, August)

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See detailPreliminary evaluation of a protocol for fast localized abdominal sonography in horses (FLASH) admitted for colic
Busoni, Valeria ULg; De Busscher, Virginie; Lopez, Diego et al

Poster (2006, August)

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See detailDiversification among damselfishes (Pomacentridae): a comparison of ontogenetic trajectories
Frederich, Bruno ULg; Vandewalle, Pierre ULg

Poster (2006, August)

The Pomacentridae family, or damselfishes, includes more than 330 species living mainly in a coral reef environment. They have a complex life cycle involving a pelagic dispersion period of larvae and a ... [more ▼]

The Pomacentridae family, or damselfishes, includes more than 330 species living mainly in a coral reef environment. They have a complex life cycle involving a pelagic dispersion period of larvae and a juvenile and adult benthic life period associated to reef. The colonisation is associated with a metamorphosis allowing the fish to adapt to their new environment. Our study focused on morphological modifications, as skeletal shape changes, occurring during the development from settling larvae to juvenile and leading to diversification of adult species. To test allometry and to examine diversification in shapes, geometric morphometrics were used. This method, which allows description and statistical analysis of form, is applied to define ontogenetic shape changes and to compare allometries responsible for species diversification. It is applied here for skull structures involved in food capture as neurocranium, suspensorium, opercle, premaxillary bone and mandible. Six species were studied: Chromis viridis, Dascyllus aruanus, Dascyllus carneus, Pomacentrus pavo, Chrysiptera glauca and Stegastes nigricans. At the end of larval stage, structures in fishes are rather similar. None of the species has an isometric growth. Ontogenetic allometries concern each skeletal unit. The two species of Dascyllus are the lone to have a common ontogenetic transformation, and it appears that allometries giving the shapes’diversification correspond to ecological adaptations of each species. From a functional point of view, the general trend in skeletal shape changes corresponds to an optimisation of the suction feeding apparatus (for example: development in height and length of the suspensorium and opercle, shortening of the mandible). [less ▲]

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See detailMapping and polymorphism of bovine ghreline gene
Colinet, Frédéric ULg; Eggen, André; Halleux, Caroline et al

Poster (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

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See detailAstroPoles : Astronomy from the Polar Plateaus; The astronomy program for the International Polar Year
Burton, M.; et al.; Surdej, Jean ULg et al

Poster (2006, August)

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See detailWithin and across breeds differences in fatty acids profiles of milk and milk fat in dairy cows
Soyeurt, Hélène ULg; Dardenne, Pierre; Mayeres, Patrick et al

Poster (2006, August)

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See detailParcellaire Forestier Informatisé
Lejeune, Philippe ULg; Rondeux, Jacques ULg

Poster (2006, July 31)

Cet outil a pour but de permettre aux propriétaires de parcelle(s) forestière(s) de déterminer un ou plusieurs objectifs de gestion et de les aider à élaborer un programme d'actions à entreprendre ... [more ▼]

Cet outil a pour but de permettre aux propriétaires de parcelle(s) forestière(s) de déterminer un ou plusieurs objectifs de gestion et de les aider à élaborer un programme d'actions à entreprendre (travaux et coupes) pour les rencontrer. [less ▲]

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See detailTest comparatif de récepteur GPS
Lejeune, Philippe ULg; Rondeux, Jacques ULg

Poster (2006, July 31)

Des tests, en conditions d'utilisation réelles, ont été réalisés en vue de comparer les performances de différents récepteurs GPS "compact"

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See detailLogiciel de cubage G-CUBE (version 1.1)
Lejeune, Philippe ULg; Rondeux, Jacques ULg

Poster (2006, July 31)

Logiciel développé dans le cadre de recherches visant à valoriser les potentialités de la forêt privée et assurer la durabilité de sa gestion.

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See detailThe Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase
Mathy, Grégory ULg

Poster (2006, July 20)

The energy-dissipating alternative oxydase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. We have found that the recombinant product is properly addressed to the mitochondria ... [more ▼]

The energy-dissipating alternative oxydase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. We have found that the recombinant product is properly addressed to the mitochondria where it was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. This analysis has shown that AOX expression affects energy-related enzymes in a very specific manner: 60 proteins exhibiting a significant difference in their abundance were identified and were implicated in major metabolic pathways such as Krebs cycle and amino-acid biosynthesis. At the level of the respiratory chain, we found a ~1.4-fold increase in the proton pumping complex III (ubiquinol-cytochrome c oxidoreductase), which competes with AOX for the reduced Q, as well as a ~1.7-fold increase in the succinate dehydrogenase that delivers electrons produced by the reduction of succinate to the Q pool. In addition, the NADH-cytochrome c reductase (MCR1) that transfers electrons from the externally-produced NADH directly to cytochrome c was ~1.4-fold greater in AOX+ mitochondria. This increase in MCR1 could force electrons to pass through the cytochrome c oxidase, the other proton pumps of the yeast respiratory chain. Up-regulation of the complex III as well as of MCR1 would influence the electron partitioning at the level of the Q pool in favour of the cytochrome pathway in order to diminish the impact of recombinant AOX on oxidative phosphorylation and energy conservation. Surprisingly, this up-regulation of the respiratory-chain was associated with a down-regulation of the ATP synthase complex. This decrease in ATP synthase content would allow the establishment of a novel steady state between the rate of ΔμH+ building and the rate of ΔμH+ consumption, favourable to the ATP synthase to perform ATP synthesis [less ▲]

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See detailLe vocabulaire de la parenté dans l'Atlas linguistique de la Wallonie
Baiwir, Esther ULg

Poster (2006, July 07)

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See detailEmotional valence influences the rates of false memories
Dehon, Hedwige ULg; Van der Linden, Martial; Laroi, Frank ULg

Poster (2006, July)

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See detailAntiprotozoal and cytotoxic triterpenoid saponins from the roots of Cephalaria gigantea
Tabatadze, Nino; Elias, Riad; Delmas, F. et al

Poster (2006, July)

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See detailEtude de la nature des unités morphologiques utilisées dans la reconnaissance des mots écrits chez les dyslexiques
Quemart, Pauline ULg; Casalis, Séverine; Colé, Pascale

Poster (2006, July)

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See detailStatistical analysis of electrophoresis results in External Quality Assessment schemes
Zhang, Lixin ULg; Libeer, Jean-Claude; Hamers, Nicole et al

Poster (2006, July)

The goal of External Quality Assessment (EQA) schemes is to ensure that results obtained on a particular specimen in a given clinical laboratory do not differ significantly from those obtained by other ... [more ▼]

The goal of External Quality Assessment (EQA) schemes is to ensure that results obtained on a particular specimen in a given clinical laboratory do not differ significantly from those obtained by other laboratories on the same specimen. Hence, EQA aims at improving inter-laboratory agreement. Classically, the same blind control specimen is sent to all laboratories and test results are returned to the EQA organizer and analyzed statistically. Robust methods are used to estimate the mean (target value) and standard deviation (SD, inter-laboratory variability) because of the frequent presence of outliers. Laboratories are considered as “poor performers” if their results depart from the target value by a given threshold (e.g., 3×SD). Serum protein electrophoresis is a laboratory test which yields five fractions (albumin, α1, α2, β and γ) which sum up to 100%. So far, EQA schemes have analyzed the five fractions separately as for ordinary tests. Thus, a laboratory is qualified as a poor performer if at least one fraction is out of range. This approach does not take into account the other fractions and the linear relationship between them. A novel statistical approach has been developed to analyze EQA electrophoresis results from a global standpoint by using multivariate standard and robust methods to eliminate the effect of outlying profiles. When applied to electrophoresis data from the Belgian EQA scheme (n = 189 laboratories), the approach has shown that other laboratories should be considered as poor performers than by the classical univariate method. It has also highlighted the need for taking into account the type of electrophoresis assay in judging the laboratory performance. The method will be implemented routinely in the Belgian EQA scheme. [less ▲]

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See detailSurgical alternative for bladder tumors involving the trigone: preliminary results in two bitches
Saulnier-Troff, François-Guillaume; Busoni, Valeria ULg; Hamaide, Annick ULg

Poster (2006, July)

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