Aspergillus fumigatus detection by PCR in broncho-alveolar fluid

Poster (1999, September 29)

Background: The usefullness of a nested PCR for detection of Aspergillus fumigatus DNA was evaluated in bronchoalveolar lavage (BAL) fluid during a period of two years (1996- 1998). The aim of the study ... [more ▼]

Background: The usefullness of a nested PCR for detection of Aspergillus fumigatus DNA was evaluated in bronchoalveolar lavage (BAL) fluid during a period of two years (1996- 1998). The aim of the study was to assess the role of PCR in diagnosing invasive pulmonary aspergillosis (IPA). Methods: A nested PCR-based amplification of fragments of genes-encoding alkaline proteases from A. fumigatus was used to test 167 BAL samples. All samples were checked for the absence of amplification inhibitors. Medical, radiological, microbiological records and autopsy findings were reviewed for assessing invasive aspergillosis. All successive patients investigated by BAL were included in the study. They were distributed in three groups: A, proven or probable aspergillosis (n=11); B, colonization (n=2); C, no evidence of IPA (n=154). PCR results were compared to culture détection as gold standard and to clinical data. Results: BAL fluids from 10 patients of group A were PCR positive. One case was falsely negative. Among group B, one case was PCR positive, and the second one PCR negative but had negative BAL cultures (only culture positive sputum). No false positive was detected among group C. Comparing to culture, sensitivity was 91%, specificity, 100%, positive predictive value, 100% and négative predictive value, 99%. Conclusion: 1. Aspergillus fumigatus PCR in BAL fluid was an accurate test to diagnose culture negative patients with IPA and to confirm culture positive samples; however it doesn't make difference between infection and colonization. 2. It is an appropriate test to exclude Aspergillus infection in patients at risk of invasive illness. [less ▲]

Comparative effects of nimesulide, nimesulide L-lysine and nimesulide L-lysine L-arginine on human articular chondrocytes in vitro

Poster (1999, September 17)

Learning new names for new stimuli : Making the connection

Poster (1999, September)

Surveillance of antimicrobial resistance in Streptococcus agalactiae isolated from patients in Belgium (1989-1991 versus 1996-1999)

Poster (1999, September)

Purification and characterization of Yarrowia lipolytica exracellular lipase.

Poster (1999, September)

Rearrangement of 1-alkynyltrialkylaluminates

Poster (1999, July 19)

Conversion of green note aldehydes into alcohols by yeast alcohol dehydrogenase

Poster (1999, July 11)

Stress-induced metabolites in potato in response to ampicillin elicitation.

Poster (1999, July)

Monitoring biomass, polysaccharides, glucose and lactate in an industrial fermentation of Streptococcus pneumoniae by Near-infrared Spectroscopic determination.

Poster (1999, July)

Evaluation of the immune response to varicella-zoster virus proteins in the elderly.

Poster (1999, July)

In vivo et in vitro effect of new glibenclamide isosteres

Poster (1999, July)

Microbiological and chemical study of « Tiéré », senegalese millet fermented food.

Poster (1999, July)

In vivo et in vitro effect of new glibenclamide isosteres

Poster (1999, July)

A soil mechanical resistance sensor for on-line application in presicion agriculture.

Poster (1999, July)

Pharmacological evaluation of 3-alkylsulfanyl-4H-1,2,4-benzothiadiazine 1,1-dioxides structurally related to the KATP channel opener diazoxide

Poster (1999, June 12)

Determination of total homocysteine in plasma by automated fluorescence polarization immunoassay

Poster (1999, June)

Study of cyclodextrin + drug systems in acidic aqueous soltuion by electrospray ionization F.T. mass spectroscopy

Poster (1999, June)

Age related memory deficits: Effects of speed manipulations during encoding and relations with controlled processes

Poster (1999, May 20)

Antifungal lipopeptides from B. subtilis induce defense-related phenolics in potato

Poster (1999, May 04)

Comparative evaluation of Fungitest, Neosensitabs and broth microdilution method for yeasts susceptibility testing

Poster (1999, May)

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical ... [more ▼]

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical isolates (26 C. albicans, 32 C. glabrata, 4 C. krusei, 2 C. tropicalis, 2 C. kefyr and 1 S. cerevisiae) to 6 drugs flucytosine (FC), amphotericin B (A), fluconazole (FZ), itraconazole (IT), kétoconazole (K), miconazole (M), comparing two methods Fungitest (Sanofi Pasteur) and Neosensitabs (Rosco). A broth microdilution adaptation from the NCCLS-M27A procedure was used as reference method. Fungitests consist of individually packed 16 wells microplates containing 6 drugs at two critical concentrations in buffered medium. Reading was performed after 24 and 48h incubation. Neosensitabs is an agar diffusion method on Shadomy agar using antifungals tablets. Reading was performed after 24h. For all strains Neosensitabs was in concordance with NCCLS M27-A for FC (94%), A (98%), FZ (55%) I (53%) with p<0.05. Fungitest correlated with NCCLS method for all antifungals after 24 and 48h incubation time (p<0.05) with respectively 95/95% for FC, 100/100% for A, 80/76% for FZ, 81/55% for I and 93/75% for K, and 88/81 for M. Candida glabrata gave the poorest result with Neosensitabs with 28% concordance for FZand 39% for I; therefore the method can't be recommended for this species. Fungitest concordance observed was 55% for I after 48h. Our results suggest that Fungitest is appropriate for routine yeast susceptibility testing. However itraconazole testing has to be improved with this method. [less ▲]

Conception, synthèse et évaluation pharmacologique de nouveaux 3-alkylamino-4H-1,2,4-benzothiadiazine 1,1-dioxydes en tant qu’activateurs potentiels des canaux potassiques ATP-dépendants

Poster (1999, May)

Impact d’antagonistes du thromboxane A2 dérivés du torasémide sur la fonction plaquettaire

Poster (1999, May)

Les 3-alkylsulfanyl-4H-1,2,4-benzothiadiazine 1,1-dioxydes structuralement apparentés au diazoxide en tant qu’activateurs potentiels des canaux potassiques sensibles à l’ATP

Poster (1999, May)

Synthesis of new copolymers of polypyrrole-poly-ε-caprolactone

Poster (1999, April 12)

Les dissections arterielles et manipulations cervicales

Poster (1999, April)

L'HACCP et la maîtrise de l'hygiène chez les artisans producteurs

Poster (1999, January 27)

Periotontitis as a risk factor for coronary heart disease

Poster (1999)

Gentle mastication induces endotoxemia in periodontitis-affected patients

Poster (1999)

Phenotypic analysis on media containing drugs

Poster (1999)

Electron-microscopic tomography of silver-stained interphase and metaphase nucleolar organizer regions

Poster (1999)

Lysosomal phospholipases inhibition by emetine, strychnopentamine and usambarensine

Poster (1999)

Extended Analysis of the Highly Ionized Neon Spectra

Poster (1999)

Morphometric study of the equine navicular bone: variations with weight

Poster (1999)

OPTIMAL LEVEL OF RAPESEED MEAL IN DIETS OF LAMBS.

Poster (1999)

Two experiments were carried out in order to improve the strategy of rapeseed meal (RPM) incorporation in the diets of lambs. In a first experiment, the effects of RPM obtained from either a high ... [more ▼]

Two experiments were carried out in order to improve the strategy of rapeseed meal (RPM) incorporation in the diets of lambs. In a first experiment, the effects of RPM obtained from either a high-glucosinolate (HG) cultivar (Honk RPM) or a double-low strain (Samourai RPM) were studied. Two types of concentrates containing 25% of RPM were compared to a Control concentrate. In a second experiment, the effects of various proportions (0 – 40%) of an industrial low glucosinolate (LG)-RPM were studied in order to determine the disorder-threshold in diets for young ruminants. One hundred forty six Texel, Suffolk or crossbred lambs ranged in age from 1 to 2 months were used. Neither the Samourai nor the Honk RPM did affect negatively animal performance whatever the parameter considered (growth, food intake and conversion, slaughter performances). Low (P < 0.05) proportions of C10:0, C12:0 and C14:0 and high contents of C18:1trans, C22:2 were determined in the perirenal fat of lambs receiving the Honk RPM. The industrial LG-RPM had no negative effects on animalperformance, best results were obtained with 25 and 30% of RPM. The Samourai RPM had no effect on the thyroid weight, whereas, the Honk RPM modified (P < 0.05) the histology of this organ; the percentages of large thyroid follicles being higher (P < 0.05) in the Honk group than in the Control and Samourai groups. High levels of Canola industrial LG-RPM(from 20%) induced also an hyperthyroidism. The Samourai and Honk RPM decreased (P < 0.05) the secretions of thyroid hormones while the industrial LG-RPM did not affect these hormones in any way. RPM had no significative effects on other hormones. It was concluded that disturbance in thyroid histology and activity induced by the ingestion of RPM did not affect the physiology and performance of lambs and that the levels between 25 to 30% of a LG-RPM were optimal for growing and fattening lambs. [less ▲]

Sites of rRNA transcript elongation in isolated nucleoli of Ehrlich tumor cells

Poster (1999)

MEASUREMENT OF AROMATIC CHOLINE ESTERS IN RAPESEED BY HPLC

Poster (1999)

Aromatic choline esters are natural compounds occurring in Brassicaceae especially in rapeseed among whom the most important is called sinapine (choline ester of the 3,5-dimethoxy-4 hydroxycinnamic acid ... [more ▼]

Aromatic choline esters are natural compounds occurring in Brassicaceae especially in rapeseed among whom the most important is called sinapine (choline ester of the 3,5-dimethoxy-4 hydroxycinnamic acid). Choline esters are easily hydrolysed by entero bacteria in digestive tractus giving the correspondent acid and a choline molecule. Choline could then be broken down, by a deaminase, into trimethylamine, compound inducing a negative flavour ("fishy odour") in the milk, the meat or in yolk. So, the method improved in our laboratory is a power tool for breeders and other scientists who would like to evaluate individual aromatic choline esters in seeds or meals. Seeds (10 g) are first ground in a coffee mill (20 sec). 200 mg of ground seeds are weighed in a test tube and then placed in a water bath (75°C). Then 10 ml of boiling methanol/acetic acid (0.05M) mixture (70/30) are added plus 0.5 ml of internal standard solution (3,5-dimethoxybenzoic choline ester, 10 µmol/ml).The heterogeneous content is stirred with a magnetic stirrer for 10 min. Only one extraction step is necessary to obtain good results. Then, 1 ml of crude extract is purified with a cation exchange column (CM Sephadex C25-120). Aromatic choline esters are eluted with acetic acid 1N. Separation and quantification of individual choline esters are further realised by high performance liquid chromatography with an Inertsil 5 ODS-2 (3 x 250 mm, 5 µm) using a ternary solvent gradient (water-acetonitrile-phosphate buffer: NaH2PO4, 20 mM at pH 2 with o-phosphoric acid). In these optimised chromatographic conditions, the choline esters are separated and quantified. The retention times and the response factors have been determinated for the 36 different choline esters (benzoic or cinnamic structures with hydroxy or methoxy groups in ortho, para or meta position) synthesised in our laboratory. [less ▲]

DETERMINATION OF GLUCOSINOLATES IN RAPESEED

Poster (1999)

The official ISO protocol (similar to U.E. recommendations established in 1987 by six european laboratories) for determination of individual glucosinolate content in rapeseed using HPLC was published for ... [more ▼]

The official ISO protocol (similar to U.E. recommendations established in 1987 by six european laboratories) for determination of individual glucosinolate content in rapeseed using HPLC was published for the first time in 1992 (ISO 9167-1). Numerous laboratories, all over the world, use now this method, especially in order to control the 00 varieties. The goals of our research were to considerably reduce the analysis time without loosing precision. A single extraction is now suggested (200 mg of ground seeds stirred in 10 ml of 75°C methanol/water 70/30 with an internal standard for 10 min). Then 1-6 ml of the crude extract is directly put on DEAE A-25 resin prepared according to the ISO method. Glucosinolates are desulfated by addition of 100 µl of concentrated Helix pomatia sulfatase (H1, EC 3.1.6.1) quickly prepared by ethanol precipitation. The desulfatation process can be reduced to 1 hour without any problems with all kind of glucosinolate (alcenyl, benzyl, indolyl, methylthio, methylsulfinyl, methylsulfonyl...). Elution of the desulfo-glucosinolates is realised with 4 x 0.5 ml of distilled water. Desulfo-glucosinolates are separated by HPLC using an Inertsil 3 ODS-3 column (100 x 3 mm, 3 µm) with a water/acetonitrile gradient (from 2 to 25% in 35 min). Resolution is very nice and the limited flow (0.4 ml/min) reduce the solvent costs and the elimination of wastes. The suggested fast method has been compared with the official ISO method analysing the three certified reference materials prepared by BCR (now IRMM) and recommended by U.E. and ISO (CRMs 366, 190 and 367). The recovery of indolyl desulfo-glucosinolates, specially 4-OH glucobrassicin, is higher with the quicker method. Results obtained with the two methods are very close for other desulfo-glucosinolates. [less ▲]

Karst granitique au Sud Cameroun. Analyses de concrétions siliceuses coralloïdes et genèse d’une grotte

Poster (1999)

Periodontitis as potential risk factor for coronary heart disease

Poster (1999)

Mastication-induced release of endotoxins in the bloodstream of periodontitis patients

Poster (1999)

La qualité : un outil de repositionnement de la viande bovine dans le marché des produits alimentaires

Poster (1999)

La qualité, dans tous les sens du terme, constitue un élément indispensable à l'avenir de la viande bovine. Pour réussir, la coopération de tous les acteurs est impérative, sous l'égide des pouvoirs ... [more ▼]

La qualité, dans tous les sens du terme, constitue un élément indispensable à l'avenir de la viande bovine. Pour réussir, la coopération de tous les acteurs est impérative, sous l'égide des pouvoirs publics. Par rapport aux autres viandes, la viande bovine possède une connotation plus "naturelle". Les consommateurs semblent prêts à assumer le coût des contrôles nécessaires. [less ▲]

Panorama des démarches qualité: à chaque stratégie son outil.

Poster (1999)

Ipsilateral responses after a stroke : an electrophysiological study by focal TMS

Poster (1999)

Effect of UV irradiation on antagonistic activity of Trichoderma sp. formulations

Poster (1999)

Use of SCAR primers for specific identification and monitoring of the antagonistic strain K of Pichia anomala

Poster (1999)

LC determination of albendazole and its main metabolites in ovine plasma using dialysis as integrated sample preparation technique

Poster (1999)

Method development and validation for the LC quantitative analysis of bacitracin in a pharmaceutical formulation

Poster (1999)

Enantiomeric separation of N-derivatised amino acids by non-aqueous capillary electrophoresis using cinchona alkaloids and derivatives as chiral selectors

Poster (1999)

Periotontitis as a risk factor for coronary heart disease

Poster (1999)

First report of indigenous canine babesiosis in Belgium

Poster (1999)