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See detailThe Dusty Young Universe: photometry and spectroscopy of quasars at z > 2
Meisenheimer, K.; Hutsemekers, Damien ULg; Tacconi, L. et al

Poster (2007)

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See detailCaractériser un profil inflammatoire grâce à l'utilisation du microdamier
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

Poster (2007)

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See detailThe Belgian guidelines for management of low back pain in occupational health
Mairiaux, Philippe ULg; Mazina, D. ULg

Poster (2007)

Aims As part of the promotion of a national strategy for the prevention and the management of acute and chronic low back pain (CLBP), the Belgian Health Care Knowledge Centre (KCE) funded a systematic ... [more ▼]

Aims As part of the promotion of a national strategy for the prevention and the management of acute and chronic low back pain (CLBP), the Belgian Health Care Knowledge Centre (KCE) funded a systematic review of the EBM literature concerning the management of LBP patients in the occupational health (OH) context. Taking account differences in regulations and practices within the health care system between EU countries1,2, this review aimed at issuing good practices recommendations applicable to the Belgian situation to prevent the transition from acute to chronic back pain and to avoid long duration absences from work. Methods The guidelines have been developed in three steps. First, an electronic search was performed for relevant guidelines and systematic reviews (SR) on the following databases: EMBASE, Medline, Cochrane Controlled Trials Register, NHS guidelines Finder, National Guidelines Clearing House, New Zealand Guidelines Group Search and Pedro database. The search strategy combined 3 groups of terms: low back pain, occupational medicine and the type of reference (practice guideline or SR). The identified guidelines were appraised using the AGREE method, while SR’s were appraised using the Cochrane Collaboration grid. In a second phase, the draft guidelines were submitted to a group of OH academics from the main Belgian universities. The revised version of the guidelines was then published in English in the KCE report 3. In a third step currently under way, the guidelines are adapted in a more practical format for facilitating their daily use by OH practitioners. This involves a working group of OH practitioners coming from 12 external prevention services and several large company OH services. The document will also be discussed with representatives of other health professionals (GP’s and medical advisors of the sickness funds). Results Scientific evidence from the 7 selected guidelines and from the 27 SR’s was organized according to the main missions of the occupational physician (OP) and medical adviser (MA). Which background information should be given to employers and employees when the OP is playing an adviser role? What evidence-based prevention policies (primary and secondary) should he/she promote in the enterprises? For the third mission of the OP, health surveillance of workers, the document considers three situations: periodic health surveillance, spontaneous examinations at the worker request, and return to work (RTW) examinations for workers absent for 28 days or more. The main conclusions are summarized in 4 tables under the format of key-points, each of those mentioning the quality of the evidence (using the Guyatt classification). Discussion and conclusions The key message for prevention of CLBP is the need for staying active and minimizing the time out of work. Evidence of high quality is in favor of a multidisciplinary approach using conservative treatments. One challenge is to avoid hospitalizations and especially surgery in CLBP patients. Those recommendations are relevant for all care settings, including the occupational environment. From a health care policy point of view, the project highlighted the major roles of the occupational physician and of the medical adviser and stressed the need to better define those roles if decision makers want to tackle the low back pain problem and the economic consequences of the related sick leave. References 1. Dutch Association of Occupational Medicine (NVAB). Management of low back workers by the occupational physician. Approved guidelines ; April 1999. 2. RCM-FOM. Occupational health guidelines for management of low back pain at work: Evidence Review and Recommendations. London, 2000. 3. Nielens H., Van Zundert J., Mairiaux P., Gailly J., Van Den Hecke N., Mazina D., Camberlin C., Bartholomeeussen S., De Gauquier K., Paulus D., Ramaekers D. Chronic Low Back Pain. Good Clinical Practice (GCP). Brussels: Belgian Health Care Knowledge Centre (KCE); 2006. KCE reports 48B (D/2006/10.273/64). [less ▲]

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See detailL'assiette du Belge en chiffres
Duquesne, Brigitte ULg

Poster (2007)

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See detailSound production by pectoral spines in Six Synodontis species (Mochokidae)
Fabri, Grégory; Mauguit, Quentin; Vandewalle, Pierre ULg et al

Poster (2007)

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See detailThe lymphatic ring assay: a new in vitro model of lymphangiogenesis
Bruyère, F; Melen, L; Blacher, Silvia ULg et al

Poster (2007)

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See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma
Henry, E.; Desmet, C. J.; Garzé, V. et al

Poster (2007)

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See detailIonoluminiscencia en minerales de interés gemológico
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Millán Chagoyén, Asunción et al

Poster (2007)

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See detailMG-63 Osteoblast culture on P culture on PLA– based copolymers for bone tissue engineering applications
Moniotte, Nicolas; Shim, Yong-Ho; Borget, Pascal et al

Poster (2007)

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common ... [more ▼]

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common technique for bone defect repairing, is associated with serious limitations, e.g. limited supply and donor site morbidity. Since a few years bone tissue engineering by degradable biomaterials has been shown as a very promising avenue for providing bone substitutes. Among these materials, bioresorbable synthetic polymers such as poly(ethylene oxide) (PEO), poly(vinyl alcohol) (PVA), poly(acrylic acid) (PAA) and poly(lactic acid) (PLA) are very appealing because their chemistry and properties are controllable and reproducible. Cellular activity and proliferation of osteosarcoma cell lines (MG-63) on films were determined by the tetrazolium salt MTT assay and by phase contrast/fluorescence microscope observations. The cytotoxicity of the materials was found to be low or negligible. Cells viability variations were observed on the surface of the films. Long-term cell culture and degradability of PLA-PEOpolymer film was investigated by optical microscopy (Giemsa staining) and environmental scanning electron microscopy (ESEM). Hydrolysis of the PLLA ester linkages led to slow film degradation. After 113 days, optical microscope observations revealed the presence of large cracks on the surface, and even breaks of small polymer fragments, while MG-63 proliferation was still very important, showing a tissue-like aspect, with extracellular matrix (ECM) deposition. These results show that PLA-PEO copolymers are very interesting bioresorbable materials for long-term bone tissue engineering applications. [less ▲]

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See detailSTAT5 is an Ambivalent Regulator of Neutrophil Homeostasis
Fievez, Laurence ULg; Desmet, Christophe ULg; Henry, E. et al

Poster (2007)

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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailFeasibility of a urine-based DNA methylation assay for early detection of bladder cancer
Renard, Isabelle; Kelly, J.; Collette, Catherine et al

Poster (2007)

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See detailDevelopment of urine-based DNA methylation assay for prostate cancer screening
Vener, T. I.; Derecho, C.; Varde, S. et al

Poster (2007)

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate ... [more ▼]

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate specific antigen (PSA) levels are the current standards for PCa early detection. However, serum PSA testing lacks both sensitivity and specificity, and core biopsies frequently fail to identify small foci of PCa. The availability of non-invasive diagnostic molecular tests that could allow for a more precise identification of malignant prostate cells in asymptomatic men would be of great clinical value to improve PCa diagnosis. Study design: 114 men scheduled to undergo a prostate biopsy were enrolled in the study. The biopsies were triggered either by an abnormally high PSA value or by suspicious findings on DRE. Patients with other known or suspected urinary malignancy were excluded from the study. Morning, post-prostate massage and post-biopsy urine samples were collected from all individuals. The main goals of this study were a) to determine if prostate massage can improve the prostate DNA quantity compared to urine collected in the morning or after biopsy, and b) to evaluate the methylation status of a gene panel in urine samples from subjects with cancer found in prostate biopsy tissue cores versus subjects without cancer. Methods: Gene promoter methylation is associated with prostate cancer and has been successfully used for the molecular detection of neoplasia in urine. We have developed real-time methylation specific PCR assays to define the methylation status of several genes. Results: Median age of the patients was 65 years (range 48-85). PCa was found in 51% of the patients. Histological diagnosis of the biopsies was compared to methylation results in urine from 102 samples (89% success rate due low DNA yields for 12 samples). The comparison between different urine sampling techniques showed that prostate massage is needed. The best results were obtained in post massage urine samples with a combination of GSTP1, p14, p16, RARβ2 and RASSF1A resulting in a sensitivity of 74% and a specificity of 75%. Future: A multiplex assay using the Cepheid SmartCycler™ II platform is under development. Further studies are in progress to validate the assay across multiple centers. [less ▲]

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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Blacher, Silvia ULg et al

Poster (2007)

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See detailThe Grolier Codex: A PIXE & RBS study of the possible Maya document
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Calderón, Tomás et al

Poster (2007)

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See detailDirecting the lipopeptide Mycosubtilin biosynthesis toward C17:0 branched isoform influences the expression of cspB and cspC in Bacillus subtilis
Guez, Jean-Sébastien; Drucbert, A.; Müller, C. et al

Poster (2007)

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailEvaluation of the antitumor activity of 16K prolactin
Kinet, Virginie; Nguyen, Ngoc-Quynh-Nhu ULg; Cornet, Anne ULg et al

Poster (2007)

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See detailThe Mus musculus Mx1 gene confers strong protection against highly pathogenic influenzavirus A H5N1
Garigliany, Mutien-Marie ULg; Lambrecht, Bénédicte; Leroy, Michaël et al

Poster (2007)

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See detailThe Aznalcóllar disaster: An in-depth PIXE study of the pirite mine spill of 1998
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Álvarez, M. A. et al

Poster (2007)

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See detailProvenance of Belgian Merovingian garnets by PIXE on IPNAS Cyclotron
Mathis, François ULg; Vrielynck, Olivier; Laclavetine, Kilian et al

Poster (2007)

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See detailFibrous mucosa preservation with NobelGuide
Bolland, Fabrice; Van Heusden, Alain ULg

Poster (2007)

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See detailUltrastructural organization of the reptilian nucleolus
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailPotential of Shallow Lake Systems to Trace Environmental Changes Caused by Earthquakes
Avsar; Boes; Hubert-Ferrari et al

Poster (2007)

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See detailMajor and trace element geochemistry in a peat core from North Poland.Preliminary results
De Vleeschouwer; Fagel, Nathalie ULg; Cherbukin et al

Poster (2007)

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See detailUnderstanding the irregularity of Seismic cycles: A Case study in Turkey- A Marie Curie Excellence Team Project
Hubert-Ferrari; Boes; Faser et al

Poster (2007)

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See detailLast two Millennia atmospheric lead and heavy metals inputs in a Belgian peat bog: regional to global Human impacts
De Vleeschouwer; Gerard; Goormaghtigh et al

Poster (2007)

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See detailAdenosine thiamine triphosphate, a new signal synthesized in response to specific forms of cellular stress in Escherichia coli
Gigliobianco, Tiziana ULg

Poster (2007)

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine ... [more ▼]

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine triphosphate (ThTP) is also found in low amounts in all organisms studied from bacteria to mammals. In E. coli ThTP accumulation is linked to amino acid starvation and the presence of a suitable carbon source. Recently a new thiamine derivative, adenosine thiamine triphosphate (AThTP) or thiaminylated ATP was discovered in our laboratory. AThTP is accumulated in E. coli during carbon starvation. When the cells are grown under optimal conditions (rich medium containing amino acids) neither ThTP nor AThTP is present in significant amounts. The synthesis of both compounds is inhibited by amino acids. In E. coli, AThTP accumulates in response to two different conditions of metabolic stress: lack of energy substrates (or inhibition of their metabolization) and uncoupled pyruvate oxidation. [less ▲]

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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailCarbon dioxide dynamics in the tropical Ebrié lagoon (Ivory Coast)
Koné, Y. J. M.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007)

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See detailComparison of different techniques for inoculation of Candidatus phytoplasma mali in biological indexing
Aldaghi, M.; Massart, Sébastien ULg; Roussel, S. et al

Poster (2007)

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See detailSIZE DISTRIBUTION OF METALLIC POWDERS : A COMPARISON OF DIGITAL IMAGING AND LASER DIFFRACTION
Gregoire, Max; Michel, Frédéric ULg; Campana, Florent et al

Poster (2007)

Automated image analysis of particles under controlled orientation (SIA) is becoming a challenging technique for laser diffraction (LD) in the field of sizing metallic particles above 5 μm. Thanks to ... [more ▼]

Automated image analysis of particles under controlled orientation (SIA) is becoming a challenging technique for laser diffraction (LD) in the field of sizing metallic particles above 5 μm. Thanks to optimal particle dispersion and fully automated microscopic imaging, it is now possible to gather individual measurements on thousands of particles within a minute. The aim of this paper is to compare results obtained with both image analysis and laser diffraction from a selection of powders (figure 1) in the range between 5 μm and 250 μm. [less ▲]

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See detailCaractérisation de la protéine 9p du virus de la varicelle et du zona (VZV)
Joris-Gerards, Aline; BONTEMS, Sébastien ULg; Di Valentin et al

Poster (2007)

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See detailFatty acids and stable isotopes in fish and harbour porpoises (Phocoena phocoena) from the North Sea: further insights in their trophic relationships
Drouguet, Olivier; Caut, Stéphane; Haelters, Jan et al

Poster (2007)

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises ... [more ▼]

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises displaying lesions linked to fishing nets has also increased this last decade. The increased abundance of porpoises together with the increase in numbers of bycaught animals raised the question of possible competition between porpoises and fishermen in the North Sea. Therefore, a detailed view on harbour porpoise trophic ecology in this region is crucial for their conservation. Stomachs of the washed ashore porpoises were often empty and provided limited information on their recent diet. Another way of looking into the diet of marine mammals is the analysis of blubber. Stable isotopes ratio (d13C and d15N, SI) and fatty acid composition (20 fatty acids, FA) were analyzed in muscle and blubber of 10 freshly stranded harbour porpoises and in 60 potential prey fish from 10 species collected in the North Sea. The SI ratio and FA composition of these fish enabled a clear classification in different trophic levels and different trophic niches. The FA composition was very similar between harbour porpoise blubber and demersal fish, underlining a preferential predation on that resource for the porpoises investigated. A bias might exist for the porpoises: most of the animals were juveniles, and washed ashore during spring. Porpoises might display other feeding habits in other life stages, other periods of the year. These preliminary results however indicate the enhanced accuracy of crossing these biomarker approaches. [less ▲]

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See detailPeculiar hydrophobic properties of the 67-78 fragment of α-synuclein are responsible for membrane destabilization and neurotoxicity
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Dupiereux-Fettweis, Ingrid ULg et al

Poster (2006, December 18)

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ... [more ▼]

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ago, tilted peptides have been detected in two other amyloidogenic proteins : the amyloid β peptide involved in Alzheimer’s disease, and the PrP protein linked to Creuztfeldt-Jakob’s disease. Tilted peptides are short protein fragments that adopt an oblique orientation when inserted into biological membranes. Tilted peptides are able to destabilize membranes. In this study, we predicted by sequence analysis and molecular modelling that the 67-78 fragment of α-synuclein is a tilted peptide. Like most of them, the α-syn 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. A mutant designed by molecular modelling to decrease the destabilizing properties of the peptide was shown to be significantly less fusogenic. The neuronal toxicity was studied using human neuroblastoma cells and we demonstrated that the α-syn 67-78 peptide induces neurotoxicity. In conclusion, we have identified a tilted peptide in α-synuclein which could be involved in the toxicity induced during amyloidogenesis of α-synuclein. [less ▲]

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See detailMolecular cloning and functional expression of a new aphid isoprenyl diphosphate synthase
Vandermoten, Sophie ULg; Beliveau, Catherine; Sen, Stephanie et al

Poster (2006, December 18)

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See detailSCREENING OF SLEEP APNEAS AND HYPOPNEAS THROUGH THE AUTOMATIC ANALYSIS OF MIDSAGITTAL JAW MOTION
Senny, Frédéric ULg; Destiné, Jacques ULg; POIRRIER, Robert ULg

Poster (2006, December 07)

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to ... [more ▼]

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to delineate events which were likely to contain the SAHs, while hidden markov models (HMMs) classified events. Considering 28 recordings from which awakenings were discarded, the method detected SAHs with a sensitivity and a specificity of 82.2% and 78.3% respectively. Obstructive, central and mixed respiratory events were distinguished fairly accurately. The jaw motion is hence a reliable marker of respiratory efforts and may suffice by itself to score SAHs. [less ▲]

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See detailImportancia de la elección de distintos oligonucleótidos para el diagnostico de BLV por PCR en productos de origen bovino
Trono, K.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2006, December 05)

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See detailInferring Groups of Correlated Failures
Lepropre, Jean; Leduc, Guy ULg

Poster (2006, December)

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur ... [more ▼]

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur nearly at the same moment in a network, it is possible that these failures have the same root cause. The input data of our algorithms are IP failure notifications that can be provided by several sources. We consider two sources: IS-IS Link State Packets (LSPs) and Syslog messages. Our first results on the Abilene and GÉANT networks show that the inference methods behave differently and that using IS-IS LSPs provides more accurate results than using Syslog messages. [less ▲]

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See detailMapping of the bovine growth hormone secretagogue receptor (GHS-R) and polymorphism study in cattle
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

Poster (2006, December)

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and ... [more ▼]

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and its subtype are abundantly located in the hypothalamus-pituitary unit, but are also distributed in other central areas and peripheral tissues. The GHS-R belongs to the G-protein coupled receptor family with seven transmembrane domain architecture. In order to determine the GHS-R gene sequence, total mRNA was extracted from abomasum and two types of GHS-R cDNA were identified. These two types are transcript variants (1a and 1b) of the same GHS-R gene. The gene encompasses two exons and a single intron. Using a 3000 Rad hybrid panel, the GHS-R gene was mapped to Bos taurus autosome 1 (BTA 1). This localization on BTA 1 agrees totally with comparative data between cattle and human since BTA 1 corresponds to part of human chromosome 3 where human GHSR is also mapped. By two-point analysis, most significantly linked marker are BL26 and BMS4031 (both LOD score : 5,66). Some studies detected different QTLs near these markers like for growth rate, carcass yield, milk portein and milk yield. In the cattle industry, it is of economical importance to increase plasma GH secretion because it is associated with faster growth, less fat stores and improved milk production. Being of economical importance and the detected QTLs near the GHS-R gene, it would be interesting to study the polymorphism on the bovine GHS-R gene. Screening for polymorphisms in the two exons on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of four single nucleotide polymorphisms (SNPs): three SNPs are in the first exon and one SNP in the second exon. In order to evaluate if GHS-R could be involved in genetic variation for growth rate, carcass yield, milk portein and milk yield, an association study between SNPs on GHS-R gene and these traits could be performed in a major cattle population. [less ▲]

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See detailA comparative study of O2 measurements in experimental (Interice II) and natural (ISPOL, Western Weddell Sea, Antarctica) first-year sea ice
Tison, Jean-Louis; Mock, T.; Thomas, David et al

Poster (2006, December)

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See detailMicro-macro mechanical modeling of bone-implant interface by means of the homogenization theory
Amor, Nadia; Van Cleynenbreugel, Tim; Geris, Liesbet ULg et al

Poster (2006, December)

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See detailOn the understanding of the physical changes in inulin powder as a function of water activity.
Ronkart, Sébastien; Paquot, Michel ULg; Goffin, Dorothée ULg et al

Poster (2006, December)

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See detailEnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; krief, Alain

Poster (2006, December)

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See detailBuilding EnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; Seleck, Caroline; Henry, Julie et al

Poster (2006, December)

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See detailInfluence de la variété de blé tendre sur les caractéristiques physico-chimiques et les propriétés techno-fonctionnelles de l'amidon
Massaux, Carine; Lenartz, Jonathan; Bodson, Bernard ULg et al

Poster (2006, November 20)

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See detailNew developments in benzopyran derivatives as pancreatic β-cell KATP channel openers
Florence, X.; De Tullio, Pascal ULg; Lebrun, P. et al

Poster (2006, November 18)

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See detailPharmacological evaluation of TP receptor antagonists by differential activity on alpha and beta isoforms
Hanson, Julien ULg; Dogne, J. M.; Ghiotto, J. et al

Poster (2006, November 18)

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See detailUso del Inmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Vissani, A.; Gutiérrez, G. et al

Poster (2006, November 12)

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov ... [more ▼]

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov.ar , Vissani Aldana1, Gutierrez Gerónimo1, Rodríguez Sabrina 1, Barrandeguy María 1 y Trono Karina 1. 1Instituto de Virología – CICVyA – INTA . Las Cabañas y Los Reseros s/n. 1712. Castelar Argentina. INTRODUCCION Y OBJETIVO. La prueba de diagnóstico “Gold Standard” para la Anemia Infecciosa Equina (AIE) continúa siendo la Inmunodifusión en gel de agar (IDGA), y aunque existen ensayos de ELISA comerciales, la mayoría de los diagnósticos se realizan en la actualidad por IDGA. En ciertas ocasiones no se puede declarar el estado serológico del animal debido a la presencia de reacciones inespecíficas que no permiten obtener un resultado concluyente por IDGA o se ha obtenido una respuesta diferente cuando se analizó la misma muestra de suero por IDGA y ELISA. Con el objetivo de contar con un método de diagnóstico que permita confirmar y calificar correctamente el estado serológico referido a la AIE, se evaluó en este trabajo un ensayo de Inmunoblot diseñado en nuestro laboratorio, que utiliza como antígeno proteína viral p26 recombinante (Inmunoblot-rp26). MATERIAL Y METODO. Un panel de sueros equinos evaluado previamente por IDGA (DyaSystems EIA-AGID test kit, Idexx Laboratorios) fue analizado por Inmunoblot-rp26 utilizando proteína recombinante p26 purificada fijada a membrana de PVDF. El panel incluyó: 74 sueros de campo positivos, 95 sueros de campo negativos, 63 sueros de campo inespecíficos, 26 sueros de equinos con anticuerpos frente a otras enfermedades infecciosas y 8 con alto grado de hemólisis. Además, se evaluaron para obtener un estándar de comparación, 5 paneles de proficiencia de 12 sueros cada uno otorgados por SENASA, 1 panel de proficiencia de 20 sueros otorgado por NVSL-USA y el suero positivo débil de referencia de la OIE para Anemia Infecciosa Equina. RESULTADOS. Los resultados obtenidos con el Inmunoblot-rp26 fueron comparados con los resultados del IDGA. Los resultados discordantes fueron analizados por Inmunoblot utilizando como antígeno la proteína de fusión en la región amino-terminal de p26 (inmunoblot-pBad). Todos los sueros positivos de campo fueron detectados como positivos por Inmunoblot-p26. De un total de 95 sueros negativos por IDGA, 6 fueron declarados como positivos por Inmunoblot-p26 y su estado confirmado por Inmunoblot-pBAD. Hubo concordancia total de resultados entre Inmunoblot-rp26 e IDGA con los paneles de proficiencia provenientes de SENASA y del NVSL. El suero de referencia de la OIE reaccionó con resultado claramente positivo, a diferencia de lo que sucede con IDGA, donde reacciona como débil positivo. Ninguno de los sueros con anticuerpos frente a otras enfermedades infecciosas, como tampoco los sueros bemolizados, presentaron reacción positiva por Inmunoblot-p26. Se obtuvo un claro resultado diagnóstico al analizar los sueros declarados “inespecíficos” por IDGA, entre los cuales se pudieron observar resultados negativos y positivos. CONCLUSION. El Inmunoblot con proteína p26 recombinante se comportó como una herramienta valiosa para el diagnostico confirmatorio de la anemia infecciosa equina. [less ▲]

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See detailEffects of vagal nerve stimulation in the rat orofacial formalin model of pain
Multon, Sylvie ULg; Scholsem, Martin; LEGRAIN, Caroline ULg et al

Poster (2006, November)

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See detailEtude de la production, la lyophilisation et la conservation d’un starter lactique du lben : lait traditionnel fermenté.
Ziada, M.; Touhami, Y.; Achour, M. et al

Poster (2006, November)

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See detailL'affirmation de soi par la pratique de l'acteur : évaluation d'un groupe thérapeutique
Delvaux, Muriel ULg; Roodthooft, Dominique; Sarto, Danielle et al

Poster (2006, November)

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de ... [more ▼]

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de techniques théâtrales à un programme de thérapies comportementales et cognitives « classique ». En expérimentant la pratique de l’acteur, ce groupe visait à permettre aux participants de s’entraîner à se mettre en éveil, d’élargir leurs champs de perceptions et de compréhensions, de prendre conscience du langage du corps en dehors de la phrase verbale, de rechercher les mécanismes qui empêchent de s’affirmer, et de mettre en place des compétences qui améliorent l’affirmation de soi et augmentent ainsi les possibilités d’action. [less ▲]

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See detailLactation effect on stable isotope ratios and mercury levels in the blood of northern elephant seals
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Poster (2006, October 27)

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios ... [more ▼]

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on day 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1 ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. To conclude, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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