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See detailAnalysis of furan by GC/MS
Scholl, Georges ULg; Eppe, Gauthier ULg; Scippo, Marie-Louise ULg et al

Poster (2007)

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See detailKarsts in sandstones and quatrzites of Minas Gerais, Brazil
Willems, Luc ULg; Rodet, J.; Pouclet, A. et al

Poster (2007)

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See detailControlled release of drugs from an original multi-component device
Nizet, dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailAn unusual cell type of the auditory organ during neonatal development: the inner pillar cells
Thelen, Nicolas ULg; Breuskin, I; Malgrange, B et al

Poster (2007)

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See detailAlien invasive species and climate change: overview of research activities
Vanderhoeven, Sonia ULg; Saad, Layla ULg; Tiébré, Marie-Solange et al

Poster (2007)

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See detailSpring CO2 dynamics within sea ice: abiotical versus biological control.
Delille, Bruno ULg; Schoemann, Véronique; Becquevort, Sylvie et al

Poster (2007)

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See detailUltrastructural organization of the nucleolus in reptiles
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailPlant-based production of human lysozyme mutants
Tocquin, Pierre ULg; Dumoulin, Mireille ULg; Dony, Nicolas ULg et al

Poster (2007)

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See detailThe XMM-LSS Survey : properties and two-point angular correlations of point-like sources
Garcet, O.; Gandhi, P.; Disseau, L. et al

Poster (2007)

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See detailRole of coactivators SRC-1 and CARM1 in estrogen receptor-alpha and beta-dependent cell proliferation in the dentate gyrus of adult female rats
Charlier, Thierry ULg; Lieblich, Stephanie E; Pawluski, Jodi L et al

Poster (2007)

Nuclear receptors such as the estrogen receptors (ER) require the presence of coactivator proteins, such as the steroid receptor coactivator (SRC-1) and coactivator-associated arginine methyltransferase ... [more ▼]

Nuclear receptors such as the estrogen receptors (ER) require the presence of coactivator proteins, such as the steroid receptor coactivator (SRC-1) and coactivator-associated arginine methyltransferase (CARM1) to enhance the transcription of target genes. Importantly, in vitro work suggests that ER􀀁 and ER􀀂 differ in the ability to recruit coactivators such as SRC-1. For example, SRC-1 has a strong affinity for ER􀀁 and a weaker affinity for ER􀀂. Interestingly, both ER􀀁 and ER􀀂 are individually involved in estradiol-enhanced cell proliferation in the dentate gyrus of adult female rats. In addition, previous work suggests a role for CARM1 in cell proliferation and for SRC-1 in cell differentiation, therefore the present study aimed to determine whether proliferating cells in the dentate gyrus of the hippocampus co-express the coactivators SRC-1 and CARM1. We also aimed to determine whether ER􀀁 and ER􀀂 agonists would result in altered expression of SRC-1 and CARM1 in new proliferating cells in the dentate gyrus. To investigate this, adult female rats were ovariectomized and treated with either the ER􀀁 agonist Propyl-pyrazole triol (PPT), the ER􀀂 agonist diarylpropionitrile (DPN), estradiol benzoate (EB), or vehicle (CTRL). Rats were then injected with BrdU (200 mg/kg) and sacrificed 24 hours later. Preliminary data suggests that DPN, PPT and EB increase cell proliferation in the dentate gyrus compared to the vehicle-injected group. Interestingly, the number of proliferating cell expressing SRC-1 is similar in all groups, suggesting that neither of the ER agonists nor EB treatment affects the co-expression of BrdU+ cells with SRC-1. However, additional measurements are currently being done to investigate whether CARM-1 is differentially expressed in proliferating cells in the hippocampus following selective ER agonist treatment. [less ▲]

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See detailThe Dusty Young Universe
Meisenheimer, K.; Dannerbauer, H.; Klaas, U. et al

Poster (2007)

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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Alvarez Gonzalez, M-L et al

Poster (2007)

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See detailGlobal morphology of substorm growth phases observed by the IMAGE-SI12 imager
Blockx, Caroline ULg; Gérard, Jean-Claude ULg; Coumans, Valérie ULg et al

Poster (2007)

Growth phases are observed to start from less than 30 minutes to over an hour before the substorm onset. The sector of maximum proton precipitation during the growth phase is generally located around 2200 ... [more ▼]

Growth phases are observed to start from less than 30 minutes to over an hour before the substorm onset. The sector of maximum proton precipitation during the growth phase is generally located around 2200 MLT. It rapidly moves in local time by about 1.2 hour toward midnight at the time of the onset. The open magnetic flux increases by as much as a 33% during the growth phase. The mean value of the open flux at the end of the growth phase, immediately preceding the substorm, onset is about 0.74. GWb for substorms triggered by external (solar wind) factors and 0.67 GWb for non-triggered substorms. The open magnetic flux generally drops following the onset of triggered substorms but continues to increase for non-triggered events. We interpret this behavior as an indication that the rate of opening of closed field lines on the dayside can exceed that of the nightside reconnection after the onset in non-triggered substorms. By contrast, flux closure is more efficient while the flux opening rate drops in the case of externally triggered onset, so that the closure rate exceeds that of field line opening on the dayside. The rate of equatorward displacement is typically ~ 3 deg/hour. It is statistically correlated with the magnitude of the southward Bz component of the IMF measured by the ACE satellite. It is also correlated with transfer functions describing the efficiency of solar wind energy transfer which involve the transverse electric field carried by the solar wind. The equatorward motion may be global, restricted to local time sectors or a combination of both. No nightside local time sector appears favored where the motion of the equatorial boundary would be more pronounced. The maximum displacement of the polar boundary is statistically located around midnight MLT. [less ▲]

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See detailEffect of aggressive encounters on plasma progesterone, corticosterone and corticosteroid binding capacity
Charlier, Thierry ULg; Hammond, Geoffrey L; Soma, Kiran K

Poster (2007)

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See detailObservations of comet McNaught from La Silla
Snodgrass, C.; Jehin, Emmanuel ULg; Fitzsimmons, A. et al

Poster (2007)

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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Blacher, Silvia ULg et al

Poster (2007)

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailCannibalism in Anchovy (Engraulis encrasicolus) in the North Aegean Sea (Greece)
Gennotte, Vincent ULg; Torre, Michele; Kallianiotis, Argyris

Poster (2007)

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 ... [more ▼]

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 % larvae. Egg consumption was observed during the night and larval consumption during the day. Cannibalism seems to be an opportunistic feeding strategy of anchovy, depending on prey availability and proximity of adults, larvae and eggs in relation with the diel vertical migrations of the fish. [less ▲]

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See detailThe Dusty Young Universe: photometry and spectroscopy of quasars at z > 2
Meisenheimer, K.; Hutsemekers, Damien ULg; Tacconi, L. et al

Poster (2007)

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See detailA TEM study of the reptilian nucleolus by means of cytochemical and immunocytological methods
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailCaractériser un profil inflammatoire grâce à l'utilisation du microdamier
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

Poster (2007)

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See detailSound production by pectoral spines in Six Synodontis species (Mochokidae)
Fabri, Grégory; Mauguit, Quentin; Vandewalle, Pierre ULg et al

Poster (2007)

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See detailL'assiette du Belge en chiffres
Duquesne, Brigitte ULg

Poster (2007)

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See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma
Henry, E.; Desmet, C. J.; Garzé, V. et al

Poster (2007)

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See detailMG-63 Osteoblast culture on P culture on PLA– based copolymers for bone tissue engineering applications
Moniotte, Nicolas; Shim, Yong-Ho; Borget, Pascal et al

Poster (2007)

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common ... [more ▼]

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common technique for bone defect repairing, is associated with serious limitations, e.g. limited supply and donor site morbidity. Since a few years bone tissue engineering by degradable biomaterials has been shown as a very promising avenue for providing bone substitutes. Among these materials, bioresorbable synthetic polymers such as poly(ethylene oxide) (PEO), poly(vinyl alcohol) (PVA), poly(acrylic acid) (PAA) and poly(lactic acid) (PLA) are very appealing because their chemistry and properties are controllable and reproducible. Cellular activity and proliferation of osteosarcoma cell lines (MG-63) on films were determined by the tetrazolium salt MTT assay and by phase contrast/fluorescence microscope observations. The cytotoxicity of the materials was found to be low or negligible. Cells viability variations were observed on the surface of the films. Long-term cell culture and degradability of PLA-PEOpolymer film was investigated by optical microscopy (Giemsa staining) and environmental scanning electron microscopy (ESEM). Hydrolysis of the PLLA ester linkages led to slow film degradation. After 113 days, optical microscope observations revealed the presence of large cracks on the surface, and even breaks of small polymer fragments, while MG-63 proliferation was still very important, showing a tissue-like aspect, with extracellular matrix (ECM) deposition. These results show that PLA-PEO copolymers are very interesting bioresorbable materials for long-term bone tissue engineering applications. [less ▲]

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See detailSTAT5 is an Ambivalent Regulator of Neutrophil Homeostasis
Fievez, Laurence ULg; Desmet, Christophe ULg; Henry, E. et al

Poster (2007)

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See detailComputed tomography anatomy of the equine metacarpo-phalangeal joint
Vanderperren; Ghaye, Benoit ULg; Hoegaerts, Michel et al

Poster (2007)

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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailFeasibility of a urine-based DNA methylation assay for early detection of bladder cancer
Renard, Isabelle; Kelly, J.; Collette, Catherine et al

Poster (2007)

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See detailDevelopment of urine-based DNA methylation assay for prostate cancer screening
Vener, T. I.; Derecho, C.; Varde, S. et al

Poster (2007)

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate ... [more ▼]

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate specific antigen (PSA) levels are the current standards for PCa early detection. However, serum PSA testing lacks both sensitivity and specificity, and core biopsies frequently fail to identify small foci of PCa. The availability of non-invasive diagnostic molecular tests that could allow for a more precise identification of malignant prostate cells in asymptomatic men would be of great clinical value to improve PCa diagnosis. Study design: 114 men scheduled to undergo a prostate biopsy were enrolled in the study. The biopsies were triggered either by an abnormally high PSA value or by suspicious findings on DRE. Patients with other known or suspected urinary malignancy were excluded from the study. Morning, post-prostate massage and post-biopsy urine samples were collected from all individuals. The main goals of this study were a) to determine if prostate massage can improve the prostate DNA quantity compared to urine collected in the morning or after biopsy, and b) to evaluate the methylation status of a gene panel in urine samples from subjects with cancer found in prostate biopsy tissue cores versus subjects without cancer. Methods: Gene promoter methylation is associated with prostate cancer and has been successfully used for the molecular detection of neoplasia in urine. We have developed real-time methylation specific PCR assays to define the methylation status of several genes. Results: Median age of the patients was 65 years (range 48-85). PCa was found in 51% of the patients. Histological diagnosis of the biopsies was compared to methylation results in urine from 102 samples (89% success rate due low DNA yields for 12 samples). The comparison between different urine sampling techniques showed that prostate massage is needed. The best results were obtained in post massage urine samples with a combination of GSTP1, p14, p16, RARβ2 and RASSF1A resulting in a sensitivity of 74% and a specificity of 75%. Future: A multiplex assay using the Cepheid SmartCycler™ II platform is under development. Further studies are in progress to validate the assay across multiple centers. [less ▲]

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See detailThe Aznalcóllar disaster: An in-depth PIXE study of the pirite mine spill of 1998
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Álvarez, M. A. et al

Poster (2007)

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See detailUltrastructural organization of the reptilian nucleolus
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

Detailed reference viewed: 4 (1 ULg)
See detailThe lymphatic ring assay: a new in vitro model of lymphangiogenesis
Bruyère, F; Melen, L; Blacher, Silvia ULg et al

Poster (2007)

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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Blacher, Silvia ULg et al

Poster (2007)

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See detailSound production mechanism in the clownfish Amphiprion clarkii (Amphiprioninae, Pomacentridae)
Colleye, Orphal ULg; Herrel, Anthony; Mauguit, Quentin et al

Poster (2007)

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic ... [more ▼]

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic interactions. Although clownfish sounds were recorded since 1930, the mechanism of sound production has remained unresolved. The sounds used to describe the sonic mechanism were directed towards hetero- and conspecifics that approach their sea anemone host. Sound recordings were synchronized using a high speed video (500 fps) coupled or not with an X-ray system. These systems allowed to quantify the movements of external and internal bones during sound production. Sounds were typically accompanied by rapid (< 30 ms) head movements such as elevation of the skull, lowering of the hyoid bar and the anterior part of the branchial basket, retraction of the pectoral girdle, and finally closing of the mouth. Synchronization of sound pulses with X-ray images indicates that sound is produced when the hyoid apparatus is completely lowered and the mouth closed by a previously unknown mechanism. Dissections of freshly dead specimens reveal an unusual ligament responsible for the rapid mouth closing. This ligament joins the hyoid bar to the internal part of the mandible. Acting as a cord, it forces the mandible to turn around its articulation during the lowering of the anterior part of the branchial basket, forcing the mouth to close. Sounds result from the collision of the jaw teeth, transferring energy to the jaws that are presumably the sound radiator. [less ▲]

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See detailEvaluation of the antitumor activity of 16K prolactin
Kinet, Virginie; Nguyen, Ngoc-Quynh-Nhu ULg; Cornet, Anne ULg et al

Poster (2007)

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See detailIonoluminiscencia en minerales de interés gemológico
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Millán Chagoyén, Asunción et al

Poster (2007)

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See detailFibrous mucosa preservation with NobelGuide
Bolland, Fabrice; Van Heusden, Alain ULg

Poster (2007)

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See detailComposition of degumming residues from oil physical refining : valorization for food application
Pierart, Céline ULg; Cavillot, Véronique; Kervyn de Meerendré, M. et al

Poster (2007)

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See detailEuroPlaNet is celebrating a very special year
Chatzchristou, E. T.; Nazé, Yaël ULg

Poster (2007)

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See detailProvenance of Belgian Merovingian garnets by PIXE on IPNAS Cyclotron
Mathis, François ULg; Vrielynck, Olivier; Laclavetine, Kilian et al

Poster (2007)

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See detailPotential of Shallow Lake Systems to Trace Environmental Changes Caused by Earthquakes
Avsar; Boes; Hubert-Ferrari et al

Poster (2007)

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See detailMajor and trace element geochemistry in a peat core from North Poland.Preliminary results
De Vleeschouwer; Fagel, Nathalie ULg; Cherbukin et al

Poster (2007)

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See detailUnderstanding the irregularity of Seismic cycles: A Case study in Turkey- A Marie Curie Excellence Team Project
Hubert-Ferrari; Boes; Faser et al

Poster (2007)

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See detailLast two Millennia atmospheric lead and heavy metals inputs in a Belgian peat bog: regional to global Human impacts
De Vleeschouwer; Gerard; Goormaghtigh et al

Poster (2007)

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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailCarbon dioxide dynamics in the tropical Ebrié lagoon (Ivory Coast)
Koné, Y. J. M.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007)

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See detailComparison of different techniques for inoculation of Candidatus phytoplasma mali in biological indexing
Aldaghi, M.; Massart, Sébastien ULg; Roussel, S. et al

Poster (2007)

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See detailCaractérisation de la protéine 9p du virus de la varicelle et du zona (VZV)
Joris-Gerards, Aline; BONTEMS, Sébastien ULg; Di Valentin et al

Poster (2007)

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See detailAdenosine thiamine triphosphate, a new signal synthesized in response to specific forms of cellular stress in Escherichia coli
Gigliobianco, Tiziana ULg

Poster (2007)

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine ... [more ▼]

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine triphosphate (ThTP) is also found in low amounts in all organisms studied from bacteria to mammals. In E. coli ThTP accumulation is linked to amino acid starvation and the presence of a suitable carbon source. Recently a new thiamine derivative, adenosine thiamine triphosphate (AThTP) or thiaminylated ATP was discovered in our laboratory. AThTP is accumulated in E. coli during carbon starvation. When the cells are grown under optimal conditions (rich medium containing amino acids) neither ThTP nor AThTP is present in significant amounts. The synthesis of both compounds is inhibited by amino acids. In E. coli, AThTP accumulates in response to two different conditions of metabolic stress: lack of energy substrates (or inhibition of their metabolization) and uncoupled pyruvate oxidation. [less ▲]

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See detailPeculiar hydrophobic properties of the 67-78 fragment of α-synuclein are responsible for membrane destabilization and neurotoxicity
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Dupiereux-Fettweis, Ingrid ULg et al

Poster (2006, December 18)

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ... [more ▼]

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ago, tilted peptides have been detected in two other amyloidogenic proteins : the amyloid β peptide involved in Alzheimer’s disease, and the PrP protein linked to Creuztfeldt-Jakob’s disease. Tilted peptides are short protein fragments that adopt an oblique orientation when inserted into biological membranes. Tilted peptides are able to destabilize membranes. In this study, we predicted by sequence analysis and molecular modelling that the 67-78 fragment of α-synuclein is a tilted peptide. Like most of them, the α-syn 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. A mutant designed by molecular modelling to decrease the destabilizing properties of the peptide was shown to be significantly less fusogenic. The neuronal toxicity was studied using human neuroblastoma cells and we demonstrated that the α-syn 67-78 peptide induces neurotoxicity. In conclusion, we have identified a tilted peptide in α-synuclein which could be involved in the toxicity induced during amyloidogenesis of α-synuclein. [less ▲]

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