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See detailThe Grolier Codex: A PIXE & RBS study of the possible Maya document
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Calderón, Tomás et al

Poster (2007)

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See detailPotential of Shallow Lake Systems to Trace Environmental Changes Caused by Earthquakes
Avsar; Boes; Hubert-Ferrari et al

Poster (2007)

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See detailMajor and trace element geochemistry in a peat core from North Poland.Preliminary results
De Vleeschouwer; Fagel, Nathalie ULg; Cherbukin et al

Poster (2007)

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See detailUnderstanding the irregularity of Seismic cycles: A Case study in Turkey- A Marie Curie Excellence Team Project
Hubert-Ferrari; Boes; Faser et al

Poster (2007)

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See detailLast two Millennia atmospheric lead and heavy metals inputs in a Belgian peat bog: regional to global Human impacts
De Vleeschouwer; Gerard; Goormaghtigh et al

Poster (2007)

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See detailThe Belgian guidelines for management of low back pain in occupational health
Mairiaux, Philippe ULg; Mazina, D. ULg

Poster (2007)

Aims As part of the promotion of a national strategy for the prevention and the management of acute and chronic low back pain (CLBP), the Belgian Health Care Knowledge Centre (KCE) funded a systematic ... [more ▼]

Aims As part of the promotion of a national strategy for the prevention and the management of acute and chronic low back pain (CLBP), the Belgian Health Care Knowledge Centre (KCE) funded a systematic review of the EBM literature concerning the management of LBP patients in the occupational health (OH) context. Taking account differences in regulations and practices within the health care system between EU countries1,2, this review aimed at issuing good practices recommendations applicable to the Belgian situation to prevent the transition from acute to chronic back pain and to avoid long duration absences from work. Methods The guidelines have been developed in three steps. First, an electronic search was performed for relevant guidelines and systematic reviews (SR) on the following databases: EMBASE, Medline, Cochrane Controlled Trials Register, NHS guidelines Finder, National Guidelines Clearing House, New Zealand Guidelines Group Search and Pedro database. The search strategy combined 3 groups of terms: low back pain, occupational medicine and the type of reference (practice guideline or SR). The identified guidelines were appraised using the AGREE method, while SR’s were appraised using the Cochrane Collaboration grid. In a second phase, the draft guidelines were submitted to a group of OH academics from the main Belgian universities. The revised version of the guidelines was then published in English in the KCE report 3. In a third step currently under way, the guidelines are adapted in a more practical format for facilitating their daily use by OH practitioners. This involves a working group of OH practitioners coming from 12 external prevention services and several large company OH services. The document will also be discussed with representatives of other health professionals (GP’s and medical advisors of the sickness funds). Results Scientific evidence from the 7 selected guidelines and from the 27 SR’s was organized according to the main missions of the occupational physician (OP) and medical adviser (MA). Which background information should be given to employers and employees when the OP is playing an adviser role? What evidence-based prevention policies (primary and secondary) should he/she promote in the enterprises? For the third mission of the OP, health surveillance of workers, the document considers three situations: periodic health surveillance, spontaneous examinations at the worker request, and return to work (RTW) examinations for workers absent for 28 days or more. The main conclusions are summarized in 4 tables under the format of key-points, each of those mentioning the quality of the evidence (using the Guyatt classification). Discussion and conclusions The key message for prevention of CLBP is the need for staying active and minimizing the time out of work. Evidence of high quality is in favor of a multidisciplinary approach using conservative treatments. One challenge is to avoid hospitalizations and especially surgery in CLBP patients. Those recommendations are relevant for all care settings, including the occupational environment. From a health care policy point of view, the project highlighted the major roles of the occupational physician and of the medical adviser and stressed the need to better define those roles if decision makers want to tackle the low back pain problem and the economic consequences of the related sick leave. References 1. Dutch Association of Occupational Medicine (NVAB). Management of low back workers by the occupational physician. Approved guidelines ; April 1999. 2. RCM-FOM. Occupational health guidelines for management of low back pain at work: Evidence Review and Recommendations. London, 2000. 3. Nielens H., Van Zundert J., Mairiaux P., Gailly J., Van Den Hecke N., Mazina D., Camberlin C., Bartholomeeussen S., De Gauquier K., Paulus D., Ramaekers D. Chronic Low Back Pain. Good Clinical Practice (GCP). Brussels: Belgian Health Care Knowledge Centre (KCE); 2006. KCE reports 48B (D/2006/10.273/64). [less ▲]

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See detailCarbon dioxide dynamics in the tropical Ebrié lagoon (Ivory Coast)
Koné, Y. J. M.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007)

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See detailAdenosine thiamine triphosphate, a new signal synthesized in response to specific forms of cellular stress in Escherichia coli
Gigliobianco, Tiziana ULg

Poster (2007)

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine ... [more ▼]

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine triphosphate (ThTP) is also found in low amounts in all organisms studied from bacteria to mammals. In E. coli ThTP accumulation is linked to amino acid starvation and the presence of a suitable carbon source. Recently a new thiamine derivative, adenosine thiamine triphosphate (AThTP) or thiaminylated ATP was discovered in our laboratory. AThTP is accumulated in E. coli during carbon starvation. When the cells are grown under optimal conditions (rich medium containing amino acids) neither ThTP nor AThTP is present in significant amounts. The synthesis of both compounds is inhibited by amino acids. In E. coli, AThTP accumulates in response to two different conditions of metabolic stress: lack of energy substrates (or inhibition of their metabolization) and uncoupled pyruvate oxidation. [less ▲]

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See detailComparison of different techniques for inoculation of Candidatus phytoplasma mali in biological indexing
Aldaghi, M.; Massart, Sébastien ULg; Roussel, S. et al

Poster (2007)

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See detailCaractérisation de la protéine 9p du virus de la varicelle et du zona (VZV)
Joris-Gerards, Aline; BONTEMS, Sébastien ULg; Di Valentin et al

Poster (2007)

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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailPeculiar hydrophobic properties of the 67-78 fragment of α-synuclein are responsible for membrane destabilization and neurotoxicity
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Dupiereux-Fettweis, Ingrid ULg et al

Poster (2006, December 18)

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ... [more ▼]

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ago, tilted peptides have been detected in two other amyloidogenic proteins : the amyloid β peptide involved in Alzheimer’s disease, and the PrP protein linked to Creuztfeldt-Jakob’s disease. Tilted peptides are short protein fragments that adopt an oblique orientation when inserted into biological membranes. Tilted peptides are able to destabilize membranes. In this study, we predicted by sequence analysis and molecular modelling that the 67-78 fragment of α-synuclein is a tilted peptide. Like most of them, the α-syn 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. A mutant designed by molecular modelling to decrease the destabilizing properties of the peptide was shown to be significantly less fusogenic. The neuronal toxicity was studied using human neuroblastoma cells and we demonstrated that the α-syn 67-78 peptide induces neurotoxicity. In conclusion, we have identified a tilted peptide in α-synuclein which could be involved in the toxicity induced during amyloidogenesis of α-synuclein. [less ▲]

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See detailMolecular cloning and functional expression of a new aphid isoprenyl diphosphate synthase
Vandermoten, Sophie ULg; Beliveau, Catherine; Sen, Stephanie et al

Poster (2006, December 18)

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See detailSCREENING OF SLEEP APNEAS AND HYPOPNEAS THROUGH THE AUTOMATIC ANALYSIS OF MIDSAGITTAL JAW MOTION
Senny, Frédéric ULg; Destiné, Jacques ULg; POIRRIER, Robert ULg

Poster (2006, December 07)

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to ... [more ▼]

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to delineate events which were likely to contain the SAHs, while hidden markov models (HMMs) classified events. Considering 28 recordings from which awakenings were discarded, the method detected SAHs with a sensitivity and a specificity of 82.2% and 78.3% respectively. Obstructive, central and mixed respiratory events were distinguished fairly accurately. The jaw motion is hence a reliable marker of respiratory efforts and may suffice by itself to score SAHs. [less ▲]

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See detailImportancia de la elección de distintos oligonucleótidos para el diagnostico de BLV por PCR en productos de origen bovino
Trono, K.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2006, December 05)

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See detailA comparative study of O2 measurements in experimental (Interice II) and natural (ISPOL, Western Weddell Sea, Antarctica) first-year sea ice
Tison, Jean-Louis; Mock, T.; Thomas, David et al

Poster (2006, December)

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See detailMapping of the bovine growth hormone secretagogue receptor (GHS-R) and polymorphism study in cattle
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

Poster (2006, December)

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and ... [more ▼]

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and its subtype are abundantly located in the hypothalamus-pituitary unit, but are also distributed in other central areas and peripheral tissues. The GHS-R belongs to the G-protein coupled receptor family with seven transmembrane domain architecture. In order to determine the GHS-R gene sequence, total mRNA was extracted from abomasum and two types of GHS-R cDNA were identified. These two types are transcript variants (1a and 1b) of the same GHS-R gene. The gene encompasses two exons and a single intron. Using a 3000 Rad hybrid panel, the GHS-R gene was mapped to Bos taurus autosome 1 (BTA 1). This localization on BTA 1 agrees totally with comparative data between cattle and human since BTA 1 corresponds to part of human chromosome 3 where human GHSR is also mapped. By two-point analysis, most significantly linked marker are BL26 and BMS4031 (both LOD score : 5,66). Some studies detected different QTLs near these markers like for growth rate, carcass yield, milk portein and milk yield. In the cattle industry, it is of economical importance to increase plasma GH secretion because it is associated with faster growth, less fat stores and improved milk production. Being of economical importance and the detected QTLs near the GHS-R gene, it would be interesting to study the polymorphism on the bovine GHS-R gene. Screening for polymorphisms in the two exons on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of four single nucleotide polymorphisms (SNPs): three SNPs are in the first exon and one SNP in the second exon. In order to evaluate if GHS-R could be involved in genetic variation for growth rate, carcass yield, milk portein and milk yield, an association study between SNPs on GHS-R gene and these traits could be performed in a major cattle population. [less ▲]

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See detailInferring Groups of Correlated Failures
Lepropre, Jean; Leduc, Guy ULg

Poster (2006, December)

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur ... [more ▼]

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur nearly at the same moment in a network, it is possible that these failures have the same root cause. The input data of our algorithms are IP failure notifications that can be provided by several sources. We consider two sources: IS-IS Link State Packets (LSPs) and Syslog messages. Our first results on the Abilene and GÉANT networks show that the inference methods behave differently and that using IS-IS LSPs provides more accurate results than using Syslog messages. [less ▲]

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See detailMicro-macro mechanical modeling of bone-implant interface by means of the homogenization theory
Amor, Nadia; Van Cleynenbreugel, Tim; Geris, Liesbet ULg et al

Poster (2006, December)

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See detailOn the understanding of the physical changes in inulin powder as a function of water activity.
Ronkart, Sébastien; Paquot, Michel ULg; Goffin, Dorothée ULg et al

Poster (2006, December)

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See detailBuilding EnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; Seleck, Caroline; Henry, Julie et al

Poster (2006, December)

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See detailEnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; krief, Alain

Poster (2006, December)

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See detailInfluence de la variété de blé tendre sur les caractéristiques physico-chimiques et les propriétés techno-fonctionnelles de l'amidon
Massaux, Carine; Lenartz, Jonathan; Bodson, Bernard ULg et al

Poster (2006, November 20)

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See detailNew developments in benzopyran derivatives as pancreatic β-cell KATP channel openers
Florence, X.; De Tullio, Pascal ULg; Lebrun, P. et al

Poster (2006, November 18)

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See detailPharmacological evaluation of TP receptor antagonists by differential activity on alpha and beta isoforms
Hanson, Julien ULg; Dogne, J. M.; Ghiotto, J. et al

Poster (2006, November 18)

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See detailUso del Inmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Vissani, A.; Gutiérrez, G. et al

Poster (2006, November 12)

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov ... [more ▼]

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov.ar , Vissani Aldana1, Gutierrez Gerónimo1, Rodríguez Sabrina 1, Barrandeguy María 1 y Trono Karina 1. 1Instituto de Virología – CICVyA – INTA . Las Cabañas y Los Reseros s/n. 1712. Castelar Argentina. INTRODUCCION Y OBJETIVO. La prueba de diagnóstico “Gold Standard” para la Anemia Infecciosa Equina (AIE) continúa siendo la Inmunodifusión en gel de agar (IDGA), y aunque existen ensayos de ELISA comerciales, la mayoría de los diagnósticos se realizan en la actualidad por IDGA. En ciertas ocasiones no se puede declarar el estado serológico del animal debido a la presencia de reacciones inespecíficas que no permiten obtener un resultado concluyente por IDGA o se ha obtenido una respuesta diferente cuando se analizó la misma muestra de suero por IDGA y ELISA. Con el objetivo de contar con un método de diagnóstico que permita confirmar y calificar correctamente el estado serológico referido a la AIE, se evaluó en este trabajo un ensayo de Inmunoblot diseñado en nuestro laboratorio, que utiliza como antígeno proteína viral p26 recombinante (Inmunoblot-rp26). MATERIAL Y METODO. Un panel de sueros equinos evaluado previamente por IDGA (DyaSystems EIA-AGID test kit, Idexx Laboratorios) fue analizado por Inmunoblot-rp26 utilizando proteína recombinante p26 purificada fijada a membrana de PVDF. El panel incluyó: 74 sueros de campo positivos, 95 sueros de campo negativos, 63 sueros de campo inespecíficos, 26 sueros de equinos con anticuerpos frente a otras enfermedades infecciosas y 8 con alto grado de hemólisis. Además, se evaluaron para obtener un estándar de comparación, 5 paneles de proficiencia de 12 sueros cada uno otorgados por SENASA, 1 panel de proficiencia de 20 sueros otorgado por NVSL-USA y el suero positivo débil de referencia de la OIE para Anemia Infecciosa Equina. RESULTADOS. Los resultados obtenidos con el Inmunoblot-rp26 fueron comparados con los resultados del IDGA. Los resultados discordantes fueron analizados por Inmunoblot utilizando como antígeno la proteína de fusión en la región amino-terminal de p26 (inmunoblot-pBad). Todos los sueros positivos de campo fueron detectados como positivos por Inmunoblot-p26. De un total de 95 sueros negativos por IDGA, 6 fueron declarados como positivos por Inmunoblot-p26 y su estado confirmado por Inmunoblot-pBAD. Hubo concordancia total de resultados entre Inmunoblot-rp26 e IDGA con los paneles de proficiencia provenientes de SENASA y del NVSL. El suero de referencia de la OIE reaccionó con resultado claramente positivo, a diferencia de lo que sucede con IDGA, donde reacciona como débil positivo. Ninguno de los sueros con anticuerpos frente a otras enfermedades infecciosas, como tampoco los sueros bemolizados, presentaron reacción positiva por Inmunoblot-p26. Se obtuvo un claro resultado diagnóstico al analizar los sueros declarados “inespecíficos” por IDGA, entre los cuales se pudieron observar resultados negativos y positivos. CONCLUSION. El Inmunoblot con proteína p26 recombinante se comportó como una herramienta valiosa para el diagnostico confirmatorio de la anemia infecciosa equina. [less ▲]

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See detailEffects of vagal nerve stimulation in the rat orofacial formalin model of pain
Multon, Sylvie ULg; Scholsem, Martin; LEGRAIN, Caroline ULg et al

Poster (2006, November)

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See detailEtude de la production, la lyophilisation et la conservation d’un starter lactique du lben : lait traditionnel fermenté.
Ziada, M.; Touhami, Y.; Achour, M. et al

Poster (2006, November)

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See detailL'affirmation de soi par la pratique de l'acteur : évaluation d'un groupe thérapeutique
Delvaux, Muriel ULg; Roodthooft, Dominique; Sarto, Danielle et al

Poster (2006, November)

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de ... [more ▼]

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de techniques théâtrales à un programme de thérapies comportementales et cognitives « classique ». En expérimentant la pratique de l’acteur, ce groupe visait à permettre aux participants de s’entraîner à se mettre en éveil, d’élargir leurs champs de perceptions et de compréhensions, de prendre conscience du langage du corps en dehors de la phrase verbale, de rechercher les mécanismes qui empêchent de s’affirmer, et de mettre en place des compétences qui améliorent l’affirmation de soi et augmentent ainsi les possibilités d’action. [less ▲]

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See detailLactation effect on stable isotope ratios and mercury levels in the blood of northern elephant seals
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Poster (2006, October 27)

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios ... [more ▼]

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on day 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1 ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. To conclude, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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See detailAnálisis filogenético de aislamientos argentinos del virus de la leucosis bovina
Rodriguez, Sabrina ULg; Trono, K.; Jones, L.R.

Poster (2006, October 22)

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un ... [more ▼]

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un retrovirus oncogénico B-linfocitotrópico que, junto con los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus, HTLV) y su contraparte en los simios (Simian T-Lymhotropic Virus, STLV), están incluidos en el género Deltaretrovirus dentro de la familia Retroviridae. BLV es un retrovirus ampliamente distribuido entre la población bovina a nivel mundial y es el agente etiológico de la leucosis bovina enzoótica (LBE), una enfermedad crónica linfoproliferativa de ocurrencia natural en el ganado bovino que constituye la enfermedad neoplásica más frecuente del ganado bovino lechero. El estudio de la diversidad genética y la comparación de aislamientos de BLV de diferentes orígenes ha sido abordado por otros autores. Sin embargo, poco se conoce acerca de la diversidad genética en aislamientos argentinos de BLV (Vet. Microbiol. 96(1):17-23; Arch.Virol.150(3):443-458). El gen env ha sido utilizado en el estudio de relaciones filogenéticas para este virus. Una gran cantidad de secuencias de este gen, correspondientes a aislamientos de BLV de distintas áreas del mundo, se encuentran disponibles en las bases de datos públicas. OBJETIVOS Estudiar la diversidad genética y las relaciones filogenéticas de los aislamientos argentinos de BLV en relación con aislamientos de otras regiones del mundo. MATERIALES Y METODOS Se utilizaron muestras clínicas provenientes de 28 bovinos adultos naturalmente infectados con BLV provenientes de distintas zonas geográficas del país como material de partida. Se diseñaron primers y se optimizaron las condiciones de una Reacción en Cadena de la Polimerasa (PCR) para la amplificación de la totalidad de la región genómica codificante para el gen env de provirus de BLV. La extracción del DNA genómico proviral se realizó a partir de tejido tumoral y de sangre entera en los casos de animales infectados sin evidencias clínicas. Los productos de PCR obtenidos fueron purificados y secuenciados directamente. La matriz de datos fue construida incluyendo las secuencias de BLV reportadas en este trabajo y secuencias correspondientes a cepas representativas de diversas regiones geográficas. Se incorporaron en el análisis secuencias homólogas de virus relacionados, las cuales fueron utilizadas como outgroup. Las secuencias fueron alineadas mediante el programa Muscle y analizadas mediante las rutinas de Parsimonia del programa TNT. Con el fin de evaluar el soporte de los grupos identificados se llevaron a cabo análisis de Bootstrap y Jackknife. CONCLUSIONES El estudio de caracterización molecular reveló una relativa estabilidad genética para los aislamientos argentinos de BLV, apoyando el hecho de que la variación genética de estos virus complejos del grupo es menor que la de otros géneros de la familia de los retrovirus. Por otra parte, del análisis filogenético se desprende el hecho de la existencia de un agrupamiento de aislamientos representativos de la región que conforman un clado único. No se observaron clados exclusivos de regiones geográficas dentro de Argentina. [less ▲]

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See detailInmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Gutiérrez, G.; Vissani, A. et al

Poster (2006, October 22)

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See detailIncorporation in bread of dietary fibres from by-products of the agro-transformation.
Roiseux, Olivier; Blecker, Christophe ULg; Sindic, Marianne ULg et al

Poster (2006, October 17)

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See detailEffect of SK channel blockade on the firing of dorsal raphe neurons in anaesthetized rats
Alleva, Livia ULg; Rouchet, Nathalie; Waroux, Olivier ULg et al

Poster (2006, October 17)

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential ... [more ▼]

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential, and therefore is important in the regulation of the firing frequency and/or pattern of many types of neurons. Serotonergic (5-HT) neurons from the raphe nuclei express SK channels and exhibit a significant AHP which can be efficiently blocked in vitro by apamin and N-methyl laudanosine (NML) (Scuvée-Moreau et al, 2004). In the later study, we found that some but not all neurons (50%) had a significantly increase in their firing rate when positive current was injected after SK channel blockade. In order to determine the physiological relevance of these channels in vivo, single unit extracellular recordings were carried out in anesthetized rats and combined with iontophoresis of the specific non-peptidic SK channel blocker, UCL1684. 5-HT neurons were tested for their inhibitory response to locally applied 5-HT and histological analysis confirmed the localization of the recording site. UCL 1684 was used at a concentration of 200 µM. Out of 11 neurons recorded, 6 showed a significant increase in the production of doublets, with no effect on their mean firing rate as compared to the control condition. The other neurons were completely unaffected. These results suggest that the responsiveness of presumed 5-HT neurons to SK channel block is variable. Although the use of 200 µM UCL allow us to be sure of a sufficient SK blockade at the recording site (Waroux et al, 2005), we can not rule out the possibility that SK channels present at the dendritic level were not completely blocked. In conclusion, SK channels in vivo might play a role in controlling the firing pattern of a subgroup of 5-HT neurons. [less ▲]

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See detailStudy of the physical changes of spray-dried inulin during storage.
Ronkart, Sébastien; Paquot, Michel ULg; Fougnies, C. et al

Poster (2006, October 17)

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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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See detailLa décomposition morphologique des mots et des pseudo-mots chez les apprentis lecteurs et les dyslexiques
Quemart, Pauline ULg; Casalis, Séverine; Mathiot, Emmanuelle

Poster (2006, October)

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See detailXylanase production by Penicillium canescens in solid-state fermentation.
Assamoi, A.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2006, October)

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See detailDistribution of nerve fibres in bovine and human mucosal associated lymphoid tissues
Defaweux, Valérie ULg; Dorban, G.; Antoine, Nadine ULg et al

Poster (2006, October)

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD ... [more ▼]

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD, but little, if any, in sCJD or BSE. In particular, the BSE strain is associated with significant PrP-res accumulation in tonsils, spleen and appendix in humans, whereas it is largely confined to the nervous system in infected cattle. Therefore, at least in the case of BSE and vCJD, it appears that host properties can influence the accumulation of the infectious agent in lymphoid organs. Mature FDC play an important role in prion pathogenesis, since neuroinvasion following peripheral challenge is significantly impaired in their absence. The proximity between these FDC and sympathetic nerve endings is known to affect the speed of prion neuroinvasion. In this study, we analysed the mucosal innervation and the interface between nerve fibres and FDC in bovine and human tonsils and in ileal and jejunal bovine Peyer’s patches using a panel of antibodies observed by confocal microscopy. Since differences in the innervation of lymphoid organs depending on age have been reported, we analysed three categories of bovine ages (new born calves, calves less than 12 months old and bovines older than 24 months) and two categories of human ages (patients less than 5 years old and patients older than 25 years). In both species, hypothetical ways of innervation by-passing germinal centre could be postulated: nerve fibres are widely distributed in antigens/cells traffic area (the lamina propria, the interfollicular zone, the suprafollicular dome in Peyer’s patches and the lymphoepithelial area in tonsils). We pointed out that, only in ileal and jejunal Peyer’s patches and in tonsils of bovines older than 24 months, nerve fibres are observed to be in contact with FDC. In contrast, in human tonsils, no nerve fibres established contact with FDC, whatever the age. Thus, innervation of germinal centres can be said to be an age-dependent dynamic process in bovines and a weak innervation of the secondary lymphoid organs could thus be a rate-limiting step to neuroinvasion in humans. This variation could influence the way of neuroinvasion and thus, the differences of susceptibility of bovines and humans to the BSE agent. [less ▲]

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See detailDiet of Damselfishes (Pomacentridae): a multidisciplinary approach
Fabri, Grégory ULg; Parmentier, Eric ULg; Lepoint, Gilles ULg et al

Poster (2006, October)

Coral reefs are the marine ecosystem showing the greatest fish diversity. Damselfishes (Pomacentridae) represent, with more than 340 species, one of the most important families in the coral reef ... [more ▼]

Coral reefs are the marine ecosystem showing the greatest fish diversity. Damselfishes (Pomacentridae) represent, with more than 340 species, one of the most important families in the coral reef environment. Currently their diet is understudied. This work has 2 aims: (i) to characterize the diet of 13 pomacentrid species of the reef of Toliara (Madagascar) and (ii) to investigate if the specific diversity of this family would result from a strong trophic segregation. A multidisciplinary approach including morphological data (teeth, lower jaw-lever mechanics and intestine length), stomach contents and stable isotope analysis were used. The morphological approach and the stomach contents show that each studied species is able to capture small planktonic preys (e.g. copepods). However, the 13 species can be divided into two trophic guilds: alguivores and planktivores (respectively species where the filamentous algae and the planktonic preys count for more than 60% of their diet). Within these two principal classes, the analysis of the stomach contents and stable isotopes permit to define sub-groups : (1) the species having a food behaviour exclusively alguivore or planktivore (> 90% of their diet) (2) species showing a more varied diet by also eating other types of preys such as vagiles and/or sessiles invertebrates. The diet would contribute but could not explain all diversity in Pomacentridae. Some species show a very similar diet. Consequently other ecological factors should be responsible for the reduction of interspecific competitions and for the existence of such diversity. [less ▲]

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See detailMelting and polymorphic behavior of binary blends made of palm oil or its fractions and other fats.
Danthine, Sabine ULg; deroanne, claude

Poster (2006, October)

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See detailPhysicochemical characterization of a dietary fibre concentrate extracted from date pulp.
Roiseux, Olivier; Blecker, Christophe ULg; Besbes, Souhail et al

Poster (2006, October)

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See detailAre CNA42 and FDC-B1 directed against ovine follicular dendritic cells?
Toppets, Vinciane ULg; Piret,J; Minne , M et al

Poster (2006, October)

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See detailStudy of the effect of exposition of MCF-7/bos cells to 17-ß-estradiol by 2D DIGE
Collodoro, Mike; Dobson, Rowan ULg; Bertrand, Virginie ULg et al

Poster (2006, October)

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that ... [more ▼]

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that will enable the detection (and eventual quantification but not identification) of numerous compounds having an estrogenic activity in a single rapid test. [less ▲]

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See detailIncorporation in bread of dietary fibres from by-products of the agro-transformations.
Roiseux, Olivier; Blecker, Christophe ULg; Vandebeke, E. et al

Poster (2006, October)

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See detailNew Cold Plasma and DLC Surface Treatments for Medical Co-Cr-Mo and Ti-6Al-4V Prostheses
Matarazzo, Lucas; Gillet, Marie-Claire ULg; Forest, B et al

Poster (2006, September 27)

Recent studies indicate that the most important factor limiting the longevity of hip prostheses is the production of wear particles from the prosthetic components. We have studied the effect of two ... [more ▼]

Recent studies indicate that the most important factor limiting the longevity of hip prostheses is the production of wear particles from the prosthetic components. We have studied the effect of two surface treatment techniques on the Ti-6Al-4V alloy: ionic nitruration (cold plasma) and Diamond-like Coating (DLC). These two techniques create a layer of 7-8 μm in depth on the surface of the alloy, modifying its structure and hardening it considerably. Biocompatibility was analyzed using cellular culture to compare viability, proliferation (DNA quantification) and differentiation (ALP assay) of human osteoblast-like cells (MG 63) as well as cellular adhesion of human fibroblasts (WI 38) or osteoblasts, under SEM. [less ▲]

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See detailConstruction d’un modèle du récepteur sérotoninergique 5HT2c
Dilly, Sébastien ULg; Farce, Amaury; Yous, Said et al

Poster (2006, September 27)

La 5-hydroxytryptamine, plus couramment appelée sérotonine, a été découverte indépendamment en 1937 dans le tractus gastro-intestinal, et en 1948 dans le sérum, puis trouvée également dans le cerveau en ... [more ▼]

La 5-hydroxytryptamine, plus couramment appelée sérotonine, a été découverte indépendamment en 1937 dans le tractus gastro-intestinal, et en 1948 dans le sérum, puis trouvée également dans le cerveau en 1953. Sa structure a pour sa part été déterminée en 1949. Cette découverte a été initialement basée sur ses propriétés contractantes sur les muscles lisses. Le système sérotoninergique est cependant beaucoup plus important que ces premières investigations ne le laissaient envisager, puisqu’il intervient pratiquement dans toutes les réponses physiologiques, tant au niveau périphérique, où la sérotonine est impliquée dans l’hypertension, l’asthme ou l’acidité gastrique, qu’au niveau central, où elle joue un rôle majeur dans la régulation de l’humeur. Cette capacité lui donne une grande importance dans le traitement des dépressions. C’est dans ce cadre que se placent nos travaux, portant plus particulièrement sur une nouvelle classe thérapeutique dont le chef de file est l’agomélatine. Cette dernière agit selon un mode d’action original en se fixant à la fois aux récepteurs mélatoninergiques et au récepteur sérotoninergique 5HT2c, ce qui a donné naissance au concept MASSA (Melatonin Agonist and Selective Serotonin Antagonist). Comme la plupart des récepteurs sérotoninergiques, le sous-type 5HT2c est un Récepteur à sept domaines transmembranaires Couplé à une Protéine G (RCPG). La structure tridimensionnelle de 5HT2c n’est pas connue expérimentalement, et nous avons donc fait appel à la modélisation par homologie comparative pour construire un modèle nous permettant d’étudier ses interactions avec l’agomélatine. [less ▲]

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See detailBiocompatibilidad de nuevos policationes sintéticos sobre las propiedades mecánicas y morfológicas de la membrana eritrocitaria
Relancio, M; Delannoy, M; Foresto, P et al

Poster (2006, September 25)

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See detailEvaluación mediante métodos ópticos de la funcionalidad de policationes sintéticos para el enmascaramiento de sitios antigénicos en el eritrocito humano
Relancio, M.F; Riquelme, B; Dumas, D et al

Poster (2006, September 25)

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See detailUtilization of lupin seed as the main protein source in broiler chicken feeding : influence of the variety and interest of protease addition
Froidmont, Eric; Beckers, Yves ULg; Dehareng, Frédéric et al

Poster (2006, September 18)

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See detailIgE controlling loci in Czech atopic patients.
Gusareva, Elena ULg; Havelková, Helena; Blažková, Hanna et al

Poster (2006, September 07)

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See detailEffects of synthetic polycations on erythrocyte membrane analyzed by optical methods
Relancio, F; Riquelme, B; Dumas, D et al

Poster (2006, September 02)

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See detailFixed implant rehabilitation of the edentulous maxilla: a systematic literature review.
LAMBERT, France ULg; Weber, HP; Gallucci, German et al

Poster (2006, September)

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See detailOptimized conditions for the preparation of Pt supported catalysts by deposition-precipitation
Cellier Caroline; Lambert, Stéphanie ULg; Ruaux Vincent et al

Poster (2006, September)

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See detailImprovement of autobiographical memory in schizophrenia patients
Blairy, Sylvie ULg; Neumann, Aurore; Pierret, Leslie et al

Poster (2006, September)

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See detailDeveloping innovatie structures and mechanisms aiming to enhance the transfert of technologies from public institutions to the private sector.
Lardinois, M.; Totte, A.; Tounkara, L. et al

Poster (2006, September)

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See detailLe NPI NH chez des sujets âgés déments et non déments
Ylieff, Michel ULg; Di Notte, David; Missotten, Pierre ULg et al

Poster (2006, September)

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See detailCharacterisation of bacteriocins from lactic acid bacteria
Dortu, C.; Franz, C.; Kostinek, M. et al

Poster (2006, September)

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See detailDendritic cells: potential actors in prion neuroinvasion.
Dorban, G.; Lallemand, C.; Defaweux, Valérie ULg et al

Poster (2006, September)

Detailed reference viewed: 11 (7 ULg)