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See detailTowards laser cooling of Iron Atoms
Huet, Nicolas ULg; Krins, Stéphanie ULg; Bastin, Thierry ULg

Poster (2011)

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See detailElectro-assisted formation of organothiols self-assembled monolayers on polycrystalline copper surfaces
Maho, Anthony ULg; Denayer, Jessica; Delhalle, Joseph et al

Poster (2011)

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See detailPoultry supply chains and demand analysis for poultry meat in the context of HPAI in Hanoi, North-Vietnam
Vu Dinh, Ton; Phan Dang, Thang; Nguyen Tuan, Son et al

Poster (2011)

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See detailSélection et traitement thermique de matériaux colorants rouges sur le site moustérien es-Skhul (ca. 100 000 B.P., Israël)
Salomon, Hélène ULg; Vignaud, Colette; d'Errico, Francesco et al

Poster (2011)

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See detailConstruction and validation of a new perceptual priming task: The contrasted word task
Geurten, Marie ULg; Meulemans, Thierry ULg

Poster (2011)

In clinical neuropsychology, different rehabilitation techniques – e.g., vanishing cues, spaced retrieval, errorless learning – developed for patients with memory impairments are considered to make use of ... [more ▼]

In clinical neuropsychology, different rehabilitation techniques – e.g., vanishing cues, spaced retrieval, errorless learning – developed for patients with memory impairments are considered to make use of the preserved implicit memory abilities shown by these patients, and notably the perceptual priming effects (Harrison et al., 2007). It might be therefore important for clinician neuropsychologists to determine whether these abilities are preserved or not in their patients. However, and quite surprisingly, assessment tools aimed at assessing implicit memory abilities in brain damaged patients are lacking. The aim of this study was to build and validate a new perceptual priming task – the contrasted word task – which could be used by neuropsychologists in their clinical practice. Seventy-two young healthy participants were included in this study, and were administered the contrasted word task in which 60 words counterbalanced in 2 orders and 3 types of priming (Visual; Auditory; New) emerged gradually from a white background on a computer screen. Subjects were asked to press the response key when they thought they had recognized the word. Results show a perceptual priming effect which is specific to the exposure modality (i.e., no inter-modality transfer), proving the efficacy of the contrasted word task to highlight a facilitated identification for words that were shown previously, and confirming the perceptual specificity of the priming effect (Schacter, 1992). [less ▲]

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See detailL'origine des hématites oolithiques exploitées durant la Préhistoire récente. Objectifs et méthodes d'un PCR
BILLARD, Cyrille; SAVARY; GOEMAERE, Eric et al

Poster (2011)

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See detailDevelopment of a LC-UV-MS analytical method for malondialdehyde.
Douny, Caroline ULg; Dure, Remy; Brose, François ULg et al

Poster (2011)

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See detailNanobodies as structural probes to investigate the mechanism of fibril formation by the amyloidogenic variants of human lysozyme
Dumont, Janice ULg; Pardon, Els; Aumont-Nicaise, Magalie et al

Poster (2011)

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidose. These ... [more ▼]

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidose. These proteins form extracellular amyloid fibrils that deposit in a wide range of tissues and organs such as liver, spleen and kidneys where they cause damages [1]. It was shown that the D67H and I56T mutations cause a loss in stability and more particularly a loss of global cooperativity of protein [1]. Consequently, under physiologically relevant conditions, these variants can transiently populate a partially unfolded state in which the beta-domain and the C-helix are cooperatively unfolded while the rest of the protein remains native like [1]. The formation of intermolecular interactions between the regions that are unfolded in this intermediate state is likely to be a fundamental trigger of the aggregation process that ultimately leads to the formation and deposition of fibrils in tissues. We have also shown that the binding of three variable domain of camelid antibodies or (VHHs) - raised against the wild type human lysozyme inhibit in vitro the formation of amyloid fibrils by the lysozyme variants. These three VHHs bind on different regions of lysozyme and act as amyloid fibrils inhibitor through different mechanisms [2, 3, and unpublished results]. In the present work, sixteen new VHHs specific of human lysozyme have been generated. Competition experiments have shown that they bind to five non overlapping epitopes. We have demonstrated that five of these new VHHs are able to bind lysozyme in conditions used for amyloid fibril formation, and interestingly two of them recognize two epitopes that are different from those of the three VHHs previously characterized [2, 3, and unpublished results]. The effects of these new VHHs on the properties of lysozyme variants such as activity, stability, cooperativity and aggregation will be discussed. [less ▲]

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See detailModernization of demand analysis for poultry meat in the context of HPAI in Hanoi, North-Vietnam
Phan Dang, Thang; Nguyen Tuan, Son; Bui Huu, Doan et al

Poster (2011)

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See detailA Chandra search for low-mass companions of late B-stars in Tr16
Evans, N. R.; DeGioia-Eastwood, K.; Gagné, M. et al

Poster (2011)

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See detailMorphology of the suspensory ligament (interosseous muscle III) of the horse
Shikh Al Sook, Mohamad Khir ULg; Espinosa, Jennifer; Piret, Joëlle ULg et al

Poster (2011)

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL ... [more ▼]

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL. The significance of “abnormal” findings is however not sufficiently known. Until now, few studies described the relationship between the ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting and staining the SL and to improve knowledge about the normal morphology of the SL. Methods: In this study, the SL of eight <sound> horses were collected. The body of the SL was divided in 3 thirds and sampling was realised within each third and between the thirds. The samples were embedded in paraffin or in Tissue-Tek for cryosections. The sections were stained with hematoxylin/eosin or Masson's trichrome. For 3 SL, ultrasounds were performed before sampling. The digital tip was maintained in physiological position owing to a press. Results: Most of the paraffin sections were shredded because of the hardness of the tissue. Cryosection revealed a better preservation of tissues. Only some freezing artifacts (holes) appeared on a few sections. Muscles fibers surrounded by adipose tissue containing blood vessels were present mainly in the proximal and medium third of the SL whereas they were not found in the distal third. The remaining structure look like a tendon and was composed of collagen fibers, stained in green with the Masson's trichrome coloration. Conclusions: This study permitted to develop cutting and staining techniques for the SL and helped to map the adipose, muscular and tendinous parts within the SL. It lays down the bases of subsequent studies that will concern ultrasonographically examined digital tips of sound and pathological horses of different breeds and ages. [less ▲]

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See detailInfection néonatale tardive : Méningite à Streptocoque Agalactiae chez un nouveau-né de 19 jours.
Bequet, Emeline ULg; Guffens, Anne; Defontaine, Elizabeth et al

Poster (2011)

Les infections à Streptocoques du groupe B (SGB ou Streptococcus Agalactiae) sont, dans 80% des cas, précoces (< 7jours de vie) et, dans 20% des cas, tardives (> 7 jours de vie). Dans cette seconde ... [more ▼]

Les infections à Streptocoques du groupe B (SGB ou Streptococcus Agalactiae) sont, dans 80% des cas, précoces (< 7jours de vie) et, dans 20% des cas, tardives (> 7 jours de vie). Dans cette seconde catégorie, 35% des infections sont des méningites et engendrent une morbidité importante chez l’enfant. Nous présentons le cas d’un garçon de 19 jours, né à terme par césarienne, ayant développé une méningite à Streptocoques B, avec un dépistage maternel négatif. A l’admission, l’enfant est septique. Un bilan complet met en évidence une méningite à Streptocoque Agalactiae. Une antibiothérapie empirique à base d’Ampicilline 200mg/kg/j, Céfotaxime 100mg/kg/j et Amikacine 15mg/kg/j est initiée. Ce traitement est maintenu 5 jours ; ensuite, poursuite du Céfotaxime en monothérapie pour une durée totale de 14 jours. Les complications précoces (présentes dans 62% des cas) ne sont pas mises en évidence par les différentes explorations réalisées : mesure du périmètre crânien ; échographie transfontanellaire à la recherche d’une hydrocéphalie ou d’abcès cérébraux ; électroencéphalogramme pour exclure des phénomènes paroxystiques. A moyen et long terme, une surveillance clinique, iconographique, ophtalmologique et de l’audiogramme devra être faite afin d’exclure un retard développemental, des troubles sensoriels ainsi qu’une hydrocéphalie, une encéphalomalacie, une atrophie cérébrale ou une porencéphalie. L’incidence des infections précoces à streptocoques B a pu être réduite grâce au dépistage maternel anténatal et à une antibioprophylaxie en cas de positivité du test. Cependant, l’administration perpartale systématique d’antibiotiques ne permet pas de diminuer le nombre et la gravité des infections tardives, la contamination étant, dans ces cas, essentiellement horizontale. Le taux de complications à 5 ans des méningites à SGB est estimé à 49%, ce qui justifie, chez ces enfants, un suivi neuropédiatrique et sensoriel à long terme. [less ▲]

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See detailResting-state fMRI activity in tinnitus
Maudoux, Audrey ULg

Poster (2011)

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See detailProduction subjective de rêve en milieu carcéral : une hypothèse adaptative
Englebert, Jérôme ULg; Gauthier, Jean-Marie ULg; Jacquemart, Catherine

Poster (2011)

Une étude sur le rêve n’est pas sans poser plusieurs questions épistémologiques et méthodologiques majeures. La première d’entre elles tient à l’essence même du rêve qui est un objet d’étude particulier ... [more ▼]

Une étude sur le rêve n’est pas sans poser plusieurs questions épistémologiques et méthodologiques majeures. La première d’entre elles tient à l’essence même du rêve qui est un objet d’étude particulier, étant strictement subjectif et difficilement objectivable. Le rêve n’existe dans la vie consciente qu’au passé ; il a une conscience uniquement rétrospective. Il existe donc un décalage temporel entre l’activité onirique et le discours sur le rêve. Ce hiatus temporel condamne donc le rêve à nous échapper dans sa finitude et pousse le chercheur à capituler face à la connaissance ultime et globale du monde imaginaire de l’individu. L’étude que nous avons réalisée se veut d’inspiration phénoménologique. Nous avons administré un questionnaire (questionnaire élaboré sous la supervision d’un collège d’experts travaillant en milieu carcéral) à une population de 48 détenus (43 hommes et 5 femmes) et à une population contrôle (appariée pour l’âge et le niveau d’étude). Le questionnaire était scindé en deux parties pour les détenus (rapport aux rêves avant l’enfermement [Gd.0] / rapport aux rêves maintenant [Gd.1]) et celui pour la population contrôle ne comprenait qu’une partie (rapport aux rêves maintenant [Gc]). Enfin, nous avons isolé trois groupes au sein de la population carcérale : Cp : Courte peine effectuée (maximum 90 jours), MP : Moyenne peine effectuée (plus de 90 jours et maximum 2 ans), LP : Longue peine effectuée (plus de 2 ans). Les résultats, qu’il conviendra de discuter dans le détail, mettent en évidence que l’activité onirique serait un mécanisme que le détenu utilise pour s’adapter à l’univers carcéral (la fréquence subjective de rêve et l’intérêt porté à ses rêves sont significativement plus importants pour le Gd.1 que le Gd.0 et le Gc). Mais ce constat tend à diminuer en fonction de la durée de la peine (au-delà de 2 ans d’enfermement [LP]). Nous pouvons expliquer cette diminution significative par un effet d’habituation (le rêve aurait permis un retour à une homéostasie psychique suffisante) ou par une abrasion du mécanisme. En effet, le rêve ne procurerait aucune modification dans le réel puisque le quotidien de l’enfermement reste le même. Privée de boucle de rétroaction, la fonction adaptative du rêve s’essoufflerait. [less ▲]

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See detailA chaque abeille son stérol et ses fleurs
Vanderplanck, Maryse ULg; Lognay, Georges ULg

Poster (2011)

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See detailSelf-DNA release mediates the adjuvant effects of aluminum salts
Marichal, Thomas ULg; Ohata, K; Bedoret, Denis et al

Poster (2011)

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See detailThick and crack-free nanocrystalline mesoporous TiO2 films obtained by capillary coating from aqueous solutions
Krins, Natacha ULg; Faustini, Marco; Louis, Benjamin et al

Poster (2011)

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See detailPostglacial pioneer settlement in the Sarvinki area, Eastern Finland : a red ochre grave in Rahakangas 1 site
Simponen, Laija; Mannermaa, Kristiina; Taipale, Noora ULg et al

Poster (2011)

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See detailUnpredictable photocatalytic ability of H2-reduced rutile-TiO2 xerogel in the degradation of dye-pollutants under UV and visible light irradiation
Páez Martínez, Carlos ULg; Lambert, Stéphanie ULg; Poelman, Dirk et al

Poster (2011)

Photocatalytic degradation of organic and inorganic pollutants on the TiO2 semiconductor has been extensively studied as a way to solve environmental problems relating to wastewater and polluted air ... [more ▼]

Photocatalytic degradation of organic and inorganic pollutants on the TiO2 semiconductor has been extensively studied as a way to solve environmental problems relating to wastewater and polluted air. Anatase and rutile are the most commonly used crystalline structures of TiO2, with anatase showing a higher photocatalytic activity attributed to its higher specific surface area and its favourable band gap energy (Eg). However, its high band gap (Eg = 3.2 eV) implies the use of UV light (lambda ≤ 380 nm) to inject electrons into the conduction band (TiO2(e-CB)) and to leave holes in the valence band (TiO2(h+VB)). Although the low band gap energy of rutile-TiO2 (Eg = 3.02 eV) allows rutile to potentially absorb more solar energy than anatase, the anatase-to-rutile phase transition leads to the collapse of the TiO2 specific surface area, which may result in a decrease in the photocatalytic activity of rutile. Low specific surface area and therefore poor absorption properties lead to strong limitations in exploring the photo-efficiency of rutile. Nevertheless, rutile has been proved to be comparable to anatase in its photoelectrochemical properties when used in dye-sensitized solar cells. In the present study, a new process for the reduction of rutile-TiO2 xerogel under hydrogen flow was developed to enhance the photocatalytic activity of TiO2 materials synthesized by the sol-gel process. So a series of H2-reduced TiO2 xerogels of low specific surface area was prepared by hydrolysis and condensation of tetraisopropoxy titanium(IV) in 2-methoxyethanol. The gels were dried under vacuum, calcined in air at different temperatures (400°C, 500°C and 700°C) and finally reduced in H2 at 400 °C. The materials were characterized by X-ray diffraction, transmission electron microscopy (TEM), FT-IR spectroscopy and UV/Visible diffuse reflectance spectroscopy. The texture was determined by nitrogen adsorption-desorption measurements. The effects of the calcination/reduction treatments on the adsorption of methylene blue (MB) in aqueous solution and on the photocatalytic degradation of MB and crystal violet (CV) under UV and visible light irradiation were also evaluated. Results showed predictable modifications in the physico-chemical properties caused by the annealing of TiO2 xerogel at high calcination temperature (700 °C), such as a total anatase-to-rutile phase transition and a considerable loss of specific surface area from 260 to 2 m2 g-1. However, the higher degree of reduction exhibited by the rutile-TiO2 lattice led to unpredictable photocatalytic activity for the dye conversion under UV and visible light irradiation: the loss of specific surface area of the rutile-TiO2 sample was compensated by the increase in the affinity of this sample for the dye. Under UV light irradiation, the rutile-TiO2 xerogel obtained after a calcination at 700 °C showed a similar level of photoactivity as the one obtained with anatase-TiO2 xerogels obtained by calcination at 400 °C and 500 °C. Under visible light, unlike anatase-TiO2 xerogels, the rutile-TiO2 xerogel showed a higher dye photoconversion rate per external surface area (40 times higher) than the commercial TiO2 Degussa P25. [less ▲]

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See detailCompelling untruths: the effect of aging on vivid false memories and memory editing
Dehon, Hedwige ULg; Lampinen, James Michael

Poster (2011)

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See detailThe influence of aromatic solvents on CuPC blue pigments in oil paintings
Defeyt, Catherine ULg; Wijnberg, Louise; Verbeeck, Muriel ULg et al

Poster (2011)

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See detailThe promotive impact of high temperature on flowering in root chicory (Cichorium intybus L.)
Mathieu, Anne-Sophie; Vandoorne, Bertrand; Quinet, Muriel et al

Poster (2011)

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See detailMesure de la lordose thoracique du porc : méthode centroïde versus méthode de Cobb
Van Cauwenberge, H; Georis, P; Busoni, Valeria ULg et al

Poster (2011)

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See detailDark matter in a SUSY left-right theory
Vicente, Avelino ULg

Poster (2011)

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See detailAqueous and non-aqueous sol-gel preparation of TiO2 films for the photocatalytic oxidation of ethanol in air
Cimieri, Iolanda; Poelman, Hilde; Lambert, Stéphanie ULg et al

Poster (2011)

One of the most versatile methods to prepare TiO2 fine powders and films is the sol-gel technique. It offers many advantages, among which the possibility to control the stoichiometry, work in mild and ... [more ▼]

One of the most versatile methods to prepare TiO2 fine powders and films is the sol-gel technique. It offers many advantages, among which the possibility to control the stoichiometry, work in mild and ambient atmospheric conditions and achieve high purity and homogeneity of the final product. Stable TiO2 sols were synthesized by an aqueous sol-gel process using titanium tetraisopropoxide (TTIP) as precursor and nitric acid in water as hydrolyzing agent. The aim of the present study was to investigate the influence of different solvents and different amounts of HNO3 on the photocatalytic activity of TiO2 in the breakdown of ethanol (EtOH) as VOC molecule under UV and visible light. In particular, ethanol, isopropanol and butanol were used as solvents and two different molar ratios of HNO3 with respect to TTIP (0.25 and 0.5 respectively) were used. Their effect was investigated with regard to the photocatalytic properties of the films obtained by spin coating on sodium free glass [1] and heat treated at 450°C in air. The microstructure, optical and morphological properties of the films were investigated using SEM, UV-Vis spectroscopy and ellipsometry. The nature of the crystalline phases was ascertained by X-ray diffraction and the surface area of the powders was measured by N2 adsorption. The porous TiO2 films obtained show quite good photocatalytic activity for the degradation of EtOH compared to titania films prepared using P25 Degussa as shown in Fig. 1. Further the photocatalytic properties of the thin films obtained by aqueous sol-gel method were compared with the activity of films prepared using the same titanium precursor by a non-aqueous sol-gel process, in which the hydrolysis is initialized by esterification reaction between acetic acid and alcohol. (Fig.1. EtOH degradation for P25 (green), non aqueous sol (purple) and aqueous sols prepared using ethanol as solvent and HNO3 in molar ratios 0.25(blue) and 0.50(red)) [1] H. Tomaszewski, K. Eufinger, H. Poelman, D. Poelman, R. De Gryse, P.F.Smet, G.B. Marin, Int. J. Photoenergy 8, 1 (2007) [less ▲]

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See detailInvasive Evaluation of Coronary Arteries with Intermediate Stenosis at Coronary CT Angiography: Prevalence of Significant Findings
GHEKIERE, O.; DEWILDE, W; COOLEN, T et al

Poster (2011)

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See detailProspective Person Memory and Retrospective Person Memory for Age Progressed Images
Lampinen, James Michael; Dehon, Hedwige ULg; Miller, Justin T.

Poster (2011)

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See detailBone regeneration using porous titanium particles vs. bovine hydroxyapatite: a sinus lift study in rabbits
LAMBERT, France ULg; LECLOUX, Geoffrey ULg; Léonard, Angélique ULg et al

Poster (2011)

Background: The use of porous titanium particles as space filling biomaterials for intraoral bone regeneration might be of interest because of both the mechanical stability and nonresorbable properties ... [more ▼]

Background: The use of porous titanium particles as space filling biomaterials for intraoral bone regeneration might be of interest because of both the mechanical stability and nonresorbable properties. Titanium particles were recently described for bone regeneration in sinus lifts and in the treatment of periimplantitis. Nevertheless, tissue integration and 3D bone regeneration with titanium particles were poorly explored in these previous reports. Aim: The first objective of this study was to qualitatively and quantitatively assess the bone formation process, particularly the long-term behavior and 3D volume stability of subsinusal bone regeneration, using titanium or bovine hydroxyapatite granules, in a rabbit model. The second objective was to evaluate the effect of the hydration of the BHA particles with a therapeutic concentration of doxycycline solution on the osteogenesis and biomaterial resorption. Methods: Rabbits underwent a double sinus lift procedure using one of three materials: grade 1 porous titanium particles (Ti), bovine hydroxyapatite (BHA) or chemically modified bovine hydroxyapatite (BHATTC). Animals were sacrificed after 1 week, 5 weeks or 6 months. Samples were analyzed using mCT and nondecalcified histology. Results: The materials used in each of the three groups allowed an optimal bone formation; bone quantities and densities were not statistically different between the three groups. At 6 months, more stable 3D volume stability was found with TI and BHATTC (P¼0.0033). At 5 weeks and 6 months, bone to material contact (BMC) corroborating osteoconduction was significantly higher with BHA and BHATTC than with Ti (P < 0.0001). Conclusions and clinical implications: Even though the studied biomaterials displayed different architectures, they are relevant candidates for sinus lift bone augmentation before dental implants because they allow adequate 3D stability and osteogenesis. However, to recommend the clinical use of Ti, both an observation on the drilling effects of Ti particles and clinical trials are needed. [less ▲]

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See detailDescription of the bone-remodeling pattern after socket preservation procedures in human: a methodological study
LAMBERT, France ULg; VINCENT, Kim ULg; VANHOUTTE, Vanessa ULg et al

Poster (2011)

Background: The bone remodeling after tooth extraction can result in the loss of as much as 50% of the buccal wall and a global crestal reduction in width of 3.87mm according to a recent systematic review ... [more ▼]

Background: The bone remodeling after tooth extraction can result in the loss of as much as 50% of the buccal wall and a global crestal reduction in width of 3.87mm according to a recent systematic review. Multiple surgical protocols using biomaterials are proposed in order to limit the typical postextraction bone resorption. However, because of the heterogeneity of the studies and, more specifically, of the assessment methods, it is difficult to assert the superiority of one technique over another. Aim: The objective of this study was to develop a new radiographic method to assess alveolar bone remodeling after socket preservation procedures. Methods: Sixteen extraction sites (in 14 patients) localized in the upper anterior maxilla were treated with bovine hydroxyapatite (0.25–1mm particles) and a saddled connective tissue graft. A radiographic 3-dimensional assessment of the hard tissues was performed at baseline and at 3 months after the procedure. Standardized horizontal measurements were taken at three corono apical levels ( 2, 5, and 9mm) and at three mesio-diatal level (Mesial, Center, and Distal) in the buccal and palatal aspects. Vertical measurements were also recorded in nine regions over the top of the alveolar crest. Results: Extraction socket-preservation technique assessed in the present study significantly reduced horizontal bone remodeling. The horizontal dimension of the crest decreased by 1.6mm (20%) in the cervical regions ( 2mm level), experienced a moderate decrease of 1mm (12%) at the 5mm level, and experienced a very low decrease of 0.5mm(6%) in the apical ( 8mm) level. The losses were always significantly higher in the buccal than in the palatal aspect. Buccally, the maximal bone remodeling at the cervical level remained below 1mm. Vertical bone resorption was homogeneous and was < 1mm in the nine measured regions. Conclusions and clinical implications: The radiographic measuring methodology proved to be accurate and reproducible. It can be applied in other clinical settings. Moreover, the surgical procedure evaluated in the present study, significantly limits the postextraction buccal bone remodeling compared with the data found in the literature for untreated extraction socket in the aesthetic area. However, a complete inhibition of the bone remodeling was not reached and the authors suggest a surgical technique using a ‘‘saddled’’ connective tissue graft to thicken buccal soft tissue biotype and consequently compensate for cervical bone loss. [less ▲]

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See detailAn investigation of the relationships between foot conformation and distal interphalangeal joint effusion in young warmblood horses at the beginning of their training
Cubeddu, Francesca; Bastianacci, Valentina; Verwilghen, Denis et al

Poster (2011)

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See detailHighly efficient organic/inorganic titania xerogels for photocatalysis applications
Bodson, Céline ULg; Bied, Catherine; Páez Martínez, Carlos ULg et al

Poster (2011)

TiO2 heterogeneous photocatalysis is an attractive technique for the complete destruction of undesirable contaminants either in aqueous or gaseous phase by using solar or artificial light illumination ... [more ▼]

TiO2 heterogeneous photocatalysis is an attractive technique for the complete destruction of undesirable contaminants either in aqueous or gaseous phase by using solar or artificial light illumination. The main drawback of TiO2 is that it can be activated only by UV light because of its large band gap (3.20 eV for anatase). Thus, it would be interesting to sensitize TiO2 to the whole visible region by doping with non-metal atoms such as phosphorus. Furthermore, it was found that the P-doped species could significantly increase the specific surface area of the materials, which consequently exhibit a higher content of surface hydroxyl groups. Upon band gap excitation of TiO2, the photoinduced electrons and positively charged holes can reduce and oxidize the species adsorbed on the TiO2 particles. The high degree of recombination between photogenerated electrons and holes is a major rate-limiting factor controlling the photocatalytic efficiency. Attempts to increase the titania efficiency have been made by doping P-doped TiO2 with metals, such as Ag. In the present study, a sol-gel method is developed to synthesize P-doped TiO2 and P/Ag-codoped xerogels by a cogelation method [1] based on the hydrolysis and the condensation of Ti(OC3H7)4 in the presence of a phosphoryled compound able to complex silver: NH2-(CH2)2-NH-(CH2)2-P(O)-(OC2H5)2, EDAP), in various alcohols. These xerogels are dried at 150°C under vacuum for 24 h, and calcined under air for 5 h at 350°C, 450°C, 550°C and 650°C. The resulting materials were characterized by ICP-AES, TG-DSC, TEM, XRD, nitrogen adsorption-desorption isotherms, FT-IR and diffuse reflectance measurements in the UV/Vis region. To measure the photoactivity of these new hybrid organic/inorganic titania xerogels, all these samples were tested for the degradation of p-nitrophenol under visible light. The titanium, phosphorus and silver contents in xerogels were confirmed by ICP-AES. As expected, theoretical and actual Ti/P molar ratio and Ag contents in xerogels are similar and then after washing with THF for 48 h. These results allow to conclude that EDAP and Ag are well incorporated within the TiO2 matrix: EDAP is anchored in the titania matrix by complexation and cogelation with TTIP [2], whereas silver, first incorporated by complexation through the ethylenediamine fragment of EDAP, is present in the xerogels in the form of nanoparticles. It was found that the phosphor-doped species could significantly increase the surface area of the materials. [2] It is noteworthy that the porosity of these materials is not affected by the addition of silver acetate during the synthesis, a very slight decrease of the specific surface area being observed in all cases. The organic/inorganic hybrid titania materials display very high photocatalytic efficiencies in the degradation of p-nitrophenol compared to pure titania. Deeper investigations concerning the structure of these materials should enable a better understanding of the photocatalytic mechanisms involved with these hybrids and will soon be reported. 1. S. Lambert, K. Y. Tran, G. Arrachart, F. Noville, C. Henrist, C. Bied, J. J. E. Moreau, M. Wong Chi Man, B. Heinrichs, Micropor. Mesopor. Mater. 2008, 115, 609. 2. C.J.Bodson1, S.D. Lambert1, C. Alié1, X. Cattoën2, J-P. Pirard1, C. Bied2, M.Wong Chi Man2, B. Heinrichs1 ,Micropor. Mesopor. Mater. 2010, 134,157 [less ▲]

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See detailDynamic Magnetic Resonance Perfusion: Influence of the Cardiac Cycle on Left Ventricle and Myocardial Signal Intensity Measurements
BROUSSAUD, T; AOUCHRIA, A; MANCINI, ISABELLE et al

Poster (2011)

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See detailPostglacial pioneer settlement in the Sarvinki area, Eastern Finland : environmental and economical setting
Pesonen, Petro; Hertell, Esa; Mannermaa, Kristiina et al

Poster (2011)

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See detailThe influence of affective valence on DRM false memories in younger and older adults
Dehon, Hedwige ULg; Laroi, Frank; Van der Linden, Martial

Poster (2011)

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See detailProduction, extraction et concentration en continu de biosurfactants produit par B. subtilis en bioréacteur à membrane
Coutte, F.; Lecouturier, D.; Leclere, V. et al

Poster (2011)

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See detailBioinformatics tools to decrypt pyoverdine biosynthesis in Pseudomonas sp.
Vanvlassenbroeck, Aurélien; Leclère, Valérie; Pupin, Maude et al

Poster (2011)

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See detailLymphangiogenesis and extracellular matrix remodeling
Erpicum, Charlotte ULg; Detry, Benoît ULg; Paupert, Jenny ULg et al

Poster (2011)

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft ... [more ▼]

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft rejection. Our laboratory has previously identified MMP2 as a key regulator of lymphangiogenesis in vitro and in vivo. However, the exact function of MMP2 in this process is yet unknown. The present work aims at elucidating the mechanisms of MMP2 action during lymphangiogenesis. MMP2 could either act as a growth factor activator or as a regulator of matrix remodeling. To address this question, we studied the effect of MMP2 on lymphangiogenesis in an novel in vitro model of sprouting cells from small aggregates (spheroids) seeded in a collagen gel. In this model, quantification of the lymphangiogenic response is performed through computerized methods allowing the measurement of the distance of migration, but also the evaluation of how the cell are migrating. We evaluated the impact of MMP2 blockage through the use of physiological (TIMP2) or chemical inhibitors or by downregulating its expression with specific siRNA. The importance of extracellular matrix composition is evaluated by embedding these spheroids into different matrices (matrigel versus collagen; pepsinized collagen versus native collagen; different collagen concentrations). Our results reveal a modification of cell migration through collagen gel after MMP2 inhibition. The utilization of DQ collagen and microscopy refractance confirmed the importance of MMP2 collagenoyitic activity for lymphangiogenesis. [less ▲]

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See detailSynthesis of amonabactins by Aeromonas hydrophila : implication of NRPS of a unique iterate-alternative type
Leclère, Valérie; Subashkumar, Rathinasamy; Wathelet, Bernard ULg et al

Poster (2011)

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See detailSynthesis of amonabactins by Aeromonas hydrophila : implication of NRPS of a unique iterative-alternative type
Leclère, Valérie; Rathisamy, Subashkumar; Wathelet, Bernard ULg et al

Poster (2011)

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See detailMassive non-thermal radio emitters: new data and their modelling
Volpi, D.; Blomme, R.; De Becker, Michaël ULg et al

Poster (2011)

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See detailDry Season Carbon Dynamics in Savannah Grassland and Rainforest Dominated River Basins of Madagascar
Marwick, T.; van Acker, K.; Darchambeau, François ULg et al

Poster (2011)

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See detailEffect of Chronic confinement stress on growth, survival, blood cortisol and glucose of perch (Perca fluviatilis)
Rougeot, Carole ULg; Tomson, Thomas; Vandecan, Michaël et al

Poster (2011)

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See detailNew Methodology to detect toxin-GPCR binding by MALDI-TOF Mass Spectrometry
Echterbille, Julien ULg; De Pauw, Edwin ULg; Gilles, Nicolas et al

Poster (2011)

Introduction More than 50 thousands of venomous species are currently indexed in the world. Each of their venoms is composed of 200 to 1000 different toxins which potentially exhibit a high selectivity ... [more ▼]

Introduction More than 50 thousands of venomous species are currently indexed in the world. Each of their venoms is composed of 200 to 1000 different toxins which potentially exhibit a high selectivity for membrane receptors such as ionic channels or G-protein coupled receptors (GPCRs). GPCRs constitute the larger family of receptors since around 800 different kinds of them are knows. GPCRs are the target of around 30% of the current pharmacopeia drugs. Notable examples include Novartis’s Zelnorm, Eli Lilly’s Zyprexa and Schering-Plough’s Clarinex used to treat constipation, schizophrenia and allergies, respectively. Finding new GPCRs ligands appears of prime interest to design new pharmacological tools and potentially discover the drugs of our future. Interestingly, several toxins from venoms have already been described to bind to this particular family of receptor, opening the way to the discovery of new peptide drugs from animal venoms1-2. This work presents a pioneering MALDI-TOF/TOF based strategy to fish new GPCRs ligands from complex mixtures such as venom fractions. Methods The proof of concept of this methodology was built by studying the binding of [Arg8]-vasopressin (AVP) on type 2-vasopressin receptor (V2). Experimentally, fragments of cellular membranes over-expressing V2 receptors were incubated with cone snail’s venom fraction (~30 peptide toxins) doped by a small amount of AVP. After 2 hours incubation, free and bound fractions were carefully purified with a combination of centrifugation and micro column purifications. Samples were finally analyzed with a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer and the resulting spectra were interpreted with FlexAnalysis (v3.0), BioTools (v3.2) and SequenceEditor (v3.2) bioinformatics’ softwares from Bruker Daltonics. Preliminary data After the incubation of cellular membranes overexpressing V2 GPCR with a complex mixture of peptides doped by AVP, we clearly detect that the only V2 ligand present in the fraction was the AVP. Our result demonstrates the possibility to identify a ligand of GPCRs from a complex peptide mixture, such as venom fractions. Contrary to radiobinding, this approach allows detecting the direct binding of the toxin and does not imply to know a ligand of the studied GPCR before starting the experiments. This opens the way to the deorphanization of receptors (180 orphans GPCRs over 800). Moreover, since the new ligand is detected by mass spectrometry, it is directly identified from the mixture, without additional purification. Its structural characterization can be directly performed by de novo sequencing experiments. The drawback of our approach is the very long (but crucial!) sample preparation as each sample requires 2 purification steps (for both free and bound fraction). The next step of our work will be the automation of the procedure to allow a high-throughput screening of venom fractions on different GPCRs and the discovery of new ligands. Novel aspect GPCR’s ligands discovery by MALDI-TOF/TOF based techniques: a new pharmacological tool. 1 Quinton, L. et al. Isolation and pharmacological characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the a1A-adrenoceptor. British Journal of Pharmacology 159, 316-325 (2010). 2 Rouget, C. et al. Identification of a novel snake peptide toxin displaying high affinity and antagonist behaviour for the α2-adrenoceptors. British Journal of Pharmacology 161, 1361-1374, doi:10.1111/j.1476-5381.2010.00966.x (2010). [less ▲]

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See detailDETERMINATION OF TRIFLURALIN RESIDUES IN CULTURED CATFISH AND WATER.
Nguyen Quoc, Thinh; Tran Minh, Phu; Do Thi Thanh, Huong et al

Poster (2011)

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See detailUne thérapie destinée aux patients schizophrènes - Self-Awareness Therapy (SAT): Résultats préliminaires
Boulanger, Marie ULg; Pauly, Marc; Nachtergael, Hilde et al

Poster (2011)

Objective: Autonoetic awareness enables to mentally relive personal events in memory to be aware of one’s own experiences across subjective time and so to have a feeling of individuality and self ... [more ▼]

Objective: Autonoetic awareness enables to mentally relive personal events in memory to be aware of one’s own experiences across subjective time and so to have a feeling of individuality and self-direction. It is intimately associated with our awareness of ourselves as persons with a past and a future. Autonoetic awareness thereof contributes to construction of our identity by the recovery of past events, particularly specific events, and the imagination of future events. According to Danion’s theoretical model (2001), autonoetic awareness impairment is at the heart of schizophrenia. This deficit should depend on defective associations between separate aspects of the events during the recall and therefore, it is associated with a reduction in accessing specific information in autobiographical memory (AM). This impairment should explain identity perturbation, cognitive deficits, negative and positive symptomatology present in schizophrenia. Supporting this model, the present study evaluates the effect of a cognitive and behavioural intervention called Self-Awareness Therapy (SAT) on autonoetic awareness, AM and the capacity to imagine oneself in the future, in schizophrenia patients. Method: Fifteen patients with schizophrenia participated to SAT and, 8 patients took part in the Integrative Program Therapy (IPT) of Brenner. Before and after therapy, patients were asked to complete questionnaires to evaluate the autonoetic awareness, AM, projection into the future as well as affective and symptomatology scales. Results: For SAT, the participants reported more specific past and future events after than before the remediation therapy. Furthermore, patients reported significantly less depressive and anxiety symptoms as well as less negative symptoms after compared before the SAT. For IPT, no significant differences emerged. Discussion: These results are in line with previous study (Blairy et al., 2008) and support the notion that, in schizophrenia the retrieving of past specifics memories and projection into the future can be improved. La conscience autonoétique (CA) nous permet de revivre mentalement des évènements personnellement vécus, d’en prendre conscience à travers un temps subjectif et de ressentir un sentiment d’individualité. Elle contribue à la construction de notre identité via la récupération d’évènements passés, particulièrement les évènements spécifiques, et l’imagination d’évènements futurs. Selon le modèle de Danion (2001), une perturbation au niveau de la CA serait au cœur de la schizophrénie. Celle-ci résulterait d’un relâchement des associations entre les différents éléments d’un évènement lors du rappel et dés lors, serait associée à une réduction de l’accès aux informations spécifiques en mémoire autobiographique. Cette perturbation pourrait expliquer les troubles de l’identité, les déficits cognitifs ainsi que la symptomatologie présents dans la schizophrénie. Afin de remédier à ces déficits, nous avons élaboré une intervention cognitive et comportementale, destinée à des patients schizophrènes, qui s’appuie sur ce modèle. Cette étude présente les résultats préliminaires de la SAT en la comparant au 1er module du programme IPT. [less ▲]

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See detailAdenylation domain specificity in pyoverdine NRPS biosynthesis
Vanvlassenbroeck, Aurélien; Leclère, Valérie; Pupin, Maude et al

Poster (2011)

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See detailAn overview of the aims and plans of an ISSI Team: Investigating the Dynamics of Planetary Magnetotails
Jackman, C.; Andre, N.; Arridge, C. et al

Poster (2011)

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See detailUsing putative anti-auxins to improve somatic embryo maturation of Abies nordmanniana
Tahiri, Abdelghani ULg; Misson, Jean-Pierre; Kevers, Claire et al

Poster (2011)

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees ... [more ▼]

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees destined in priority to the export market. In vitro propagation by somatic embryogenesis is considered as an alternative method to seedlings for producing homogeneous and orthotropic trees on a large scale. Currently, the performances of the somatic embryo (SE) maturation is still insufficient to consider any commercial application of the technology. In this way , the effect of three substances  (2,3,5-triiodobenzoïque acid  as TIBA, 2 - (p-chlorophenoxy)-2-methylpropionic acids as PCIB, p-hydroxbenzoïque as PHBA)  reported as ''anti-auxins'' have been tested with three embryogenic lines on the formation and maturation of normal somatic embryos. [less ▲]

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See detailCaractérisation des pigments préhistoriques par analyse PIXE
Lebon, Matthieu; Beck, Lucile; Lahlil, Sophia et al

Poster (2011)

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See detailSedimentology and magnetic susceptibility on a continuous Lower Givetian to Lower Famennian fore-reef succession (Sauerland, Germany) : A new example which reinforce the use of MS for long-distance correlations (Germany, Belgium and Moravia).
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Boulvain, Frédéric ULg et al

Poster (2011)

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick ... [more ▼]

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick) covers a well constraint stratigraphic interval starting at the base of the Givetian (Stritzke 1991;Aboussalam et al., 2003) and according to Stritzke (1991) and our new datations ending within the Lower Famennian. The Middle-Upper Devonian shelf-edge within the Rheinisches Schiefergebirge can be traced from the supposed position along the southern rim of the Dinant Syncline and the Eifel Synclines, northwards along a line connecting the southern margin of the Devonian reefal outcrops of Attendorn and Brilon (Krebs 1967, 1974). The depositional setting of the investigated section corresponds to complex slope and basinal environments where reworked material from the proximal Brilon platform (located to the north) and basin deposits coexist. Thus, this section allows to follow the evolution of the Givetian-Frasnian Brilon platform (e.g., Machel 1990; Stritze 1990, 1991) in a deeper setting. Petrographic analysis of more than 330 thin-sections leads to the identification of 7 microfacies which are integrated into a palaeoenvironmental model. Microfacies curve evolution shows two main trends. A shallowing upward trend ending within a typical proximal slope setting (dismantling of the platform) followed by a deepening upward trend which is characterized by several meter of pelagic mudstone within the upper part of the studied section. Magnetic susceptibility variations in sedimentary rocks, have commonly been interpreted as related to variations of detritic inputs through climatic or sea level changes (Crick et al., 1994). The magnetic susceptibility (MS) study of more than 330 samples from this long-time fore-reef carbonated succession is an opportunity to better constraint our sedimentological interpretations. To do so, we propose a comparison between general MS trends and some parameters such as microfacies and relative sea level fluctuations interpreted on the basis of the sedimentological study. The relatively long stratigraphic interval covered by the Burgberg section offers a good opportunity to compare our data with the time equivalent Devonian sections of the Ardennes (Belgium) and Moravian karst area (Czech Republic) (Boulvain et al., 2010). And thus to test the magnetic susceptibility tool for long-distance correlation between stratigraphically well constraint sections. [less ▲]

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See detailLe Magdalénien dans le Nord-Ouest de l'Europe
Miller, Rebecca ULg; Noiret, Pierre ULg

Poster (2011)

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See detailSeed dispersal by western lowland gorillas (G. g. gorilla) in south east Cameroon
Petre, Charles-Albert ULg; Tagg, Nikki; Beudels-Jamar, Roseline et al

Poster (2011)

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See detailImpact of the treatment conditions of a formic/acetic acid delignification method on chemical structure and antioxidant activity of beech wood lignin
Simon, Mathilde ULg; Vanderghem, Caroline ULg; Richel, Aurore ULg et al

Poster (2011)

The interest in lignocellulosic substrates is increasing because they are clearly identified for their high potential of development in energy and value-added molecules production. Indeed lignocellulosic ... [more ▼]

The interest in lignocellulosic substrates is increasing because they are clearly identified for their high potential of development in energy and value-added molecules production. Indeed lignocellulosic biomass constitutes a promising resource for a sustainable production of organic compounds and biobased products that could progressively replace molecules from the petrochemical industry. Until now lignocellulosic substrates were mainly used for the valorization of cellulose. Hemicelluloses and lignins were less valorized and often degraded after the process. However, due to their phenolic structure, lignins can be valorized in a lot of high-valued applications like vanillin production, replacement of petrochemical polymers, antioxidants for cosmetics and food industry, resins… In this study, a representative sample of beech wood (Fagus sylvatica L.) was collected in the region of Gaume in Belgium. Beech wood was delignified at atmospheric pressure by an organosolv process using a mixture of formic acid/acetic acid/water. The effect of cooking time and temperature was evaluated on the structure, physico-chemical properties and antioxidant activity of the lignins obtained from the black liquor after treatments. The structural and physico-chemical characteristics of the lignins were investigated with different tools like infrared spectrometry, thermogravimetric analysis, gel permeation chromatography after acetylation of lignins, NMR 1H, 13C and HSQC. The antioxidant activity was assessed by a spectrophotometric method using the α,α-diphenyl-β-picrylhydrazyl radical (DPPH). [less ▲]

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See detailGait feature extraction in Parkinson's disease using low-cost accelerometers
Stamatakis, Julien; Cremers, Julien ULg; Maquet, Didier ULg et al

Poster (2011)

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling ... [more ▼]

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling, short steps, freezing of gait (FoG) and/or asymmetries in gait. There are currently no validated clinical instruments or device that allow a full characterization of gait disturbances in PD. As a step towards this goal, a four accelerometer-based system is proposed to increase the number of parameters that can be extracted to characterize parkinsonian gait disturbances such as FoG or gait asymmetries. After developing the hardware, an algorithm has been developed, that automatically epoched the signals on a stride-by-stride basis and quantified, among others, the gait velocity, the stride time,the stance and swing phases, the single and double support phases or the maximum acceleration at toe-off, as validated by visual inspection of video recordings during the task. The results obtained in a PD patient and an healthy volunteer are presented. The FoG detection will be improved using time-frequency analysis and the system is about to be validated with a state-of-the-art 3D movement analysis system. [less ▲]

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