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See detailReduced specificity of autobiographical memory and of personal projection in the future in alcoholics
Blairy, Sylvie ULg; Neumann, Aurore; Vanlofvelde, Cécile

Poster (2007, July)

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See detailCromakalim analogues as pancreatic β-cell-selective KATP channel openers
Florence, X.; De Tullio, Pascal ULg; Lebrun, P. et al

Poster (2007, July)

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See detailIgf2 expression is required for complete immunological tolerance to insulin
Hansenne, Isabelle; Renard, Chantal; Geenen, Vincent ULg

Poster (2007, July)

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See detailMorphological decomposition in visual word recognition in French beginning readers
Quemart, Pauline ULg; Casalis, Séverine; Mathiot, Emmanuelle

Poster (2007, July)

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See detailMeasurement of the partial pressure of CO2 in bulk sea ice
Geilfus, Nicolas-Xavier ULg; Verbeke, Véronique; Delille, Bruno ULg et al

Poster (2007, July)

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See detailDevelopment and pre-validation of a high sensitive method for the determination of levonorgestrel in human plasma by SPE/LC/MS-MS
Hubert, Cédric ULg; streel, Bruno; Sibenaler, Renilde et al

Poster (2007, July)

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See detailThrombin activity profile : a powerful ex vivo screening test for the development of anticoagulant drugs
Robert, S.; Ghiotto, J.; Pirotte, Bernard ULg et al

Poster (2007, July)

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See detailKey roles for metal transport proteins in naturally selected Zn/Cd hyperaccumulation and tolerance in A. halleri
Talke, Ina N.; Hanikenne, Marc ULg; Ertych, N. et al

Poster (2007, June 26)

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See detailBm-573, a thromboxane receptor antagonist, reduces development of atherosclerosis in apoe–deficient mice
Cherdon, Céline ULg; Rolin, Stéphanie; Hanson, Julien ULg et al

Poster (2007, June 22)

Atherosclerotic cardiovascular disease, according to World Health Organization, is the primary cause of heart disease and stroke. Atherosclerosis is a chronic vascular disease whose development is ... [more ▼]

Atherosclerotic cardiovascular disease, according to World Health Organization, is the primary cause of heart disease and stroke. Atherosclerosis is a chronic vascular disease whose development is influenced by several mediators. Among them, the action of eicosanoïds such as thromboxane A2 and 8-iso-PGF2a have recently received a lot of attention. The aim of our study was the evaluation of benefits of original molecules, synthesised in our lab, targeting the thromboxane receptor (TP) in an apo E deficient mouse. We previously demonstrated in several in vitro and in vivo pharmacological experiments that our original sulfonylurea derivate, BM-573 was a potent combined inhibitor of the thromboxane synthase and antagonist of TP. Since TP is implied in atherosclerosis development, such antagonist could have a great therapeutic impact in atherogenesis.To test the efficacy of BM-573 in atherogenesis, the effect of 10 weeks of treatment with BM573 (10 mg/kg) on early aortic atherosclerotic lesions of apo E deficient mice was assessed. These mice were fed with chow diet, with spontaneous increase of total plasma cholesterol and triglycerides. In this experiment, while BM-573 did not affect body weight, it significantly decreased early atherogenesis lesions confirmed by macroscopic, microscopic and biochemical analysis. These results confirm that selective antagonism of TP receptor is effective in reducing atherosclerotic lesion in apo E deficient mice. Consequently, BM-573 could be a potential drug for prevention of atherosclerosis. [less ▲]

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See detailA conceptual tool for successful participation
Cornélis, Bernard; Brunet, Sébastien ULg; Delvenne, Pierre ULg et al

Poster (2007, June 19)

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See detailTHIXOFORMAGE DES METAUX: Mise en forme rapide de pièces fonctionelles “near-net-shape” à design complexe dignes du forgeage!
Rassili, Ahmed ULg; Vaneetveld, Grégory ULg; Pierret, Jean-Christophe ULg et al

Poster (2007, June 14)

Présentation du laboratoire de thixofomage de métaux.

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See detailDevelopment of a new integrated biosensor system for an accurate diagnosis of prostate cancer using optoacoustic detection
Fleron, Maximilien ULg; Schol, Daureen ULg; Greisch, Jean-François et al

Poster (2007, June 09)

The prostate cancer is the most common male-specific cancer observed in the European Union and is the second leading cause of cancer death in men in our industrialized countries. The choice of treatment ... [more ▼]

The prostate cancer is the most common male-specific cancer observed in the European Union and is the second leading cause of cancer death in men in our industrialized countries. The choice of treatment and its efficiency is largely dependent on the stage and on the degree of advancement of the cancer when it is diagnosed. Screening procedures like digital rectal examination (DRE) and free prostate specific antigen (PSA) level testing are well established but lack accuracy, yielding only 80% of prostate cancers diagnosed in an early stage. By providing a more accurate and precise tool for diagnosing prostate cancer in its early stages, the percentage of curable cancer patients would increase radically. Current imaging techniques have limited value, thus a major challenge in current prostate cancer oncology is to develop more accurate imaging assessments. An efficient imaging technique which significantly improves the sensitivity and specificity of diagnosing, staging and predicting the behaviour of prostate cancer would be extremely valuable. The ADONIS Project intends to prove the concept of using optoacoustic imaging in combination with biologically functionalized nanoparticles as an integrated biosensor based system for the production of specific and sensitive data for accurate diagnosis of prostate cancer. The achievement of this objective requires excellent know-how on a variety of scientific and technologic fields, brought by the partners of ADONIS, coming from five European countries, such as laser and ultrasound technologies and image reconstruction techniques, the bio-functionalization of nanoparticles, the system integration and, finally, experiments and competent evaluation of the results for their application potential. The development of the biosensor is firstly performed to target the Prostate Specific Membrane Antigen (PSMA), a transmembrane protein considered as a suitable biomarker for prostate cancer and which is under intense investigation for use as an imaging and therapeutic target. To allow the detection optimization of the biosensor, a 3D cellular culture technique (Rotating Cell Culture System) is developed with LNCaP cells (a human prostate carcinoma cell line reported to express PSMA) to be closest to the in vivo aspect for which a three-dimensional aspect of tumor for the biosensor detection is needed. Detection and localisation of PSMA on LNCaP cell surface was performed by immunostainning on monolayer culture and on spheroid slices. Then, by backscattered electron (BSE) microscopy analysis, detection of nanoparticles on cells surface shows the successful binding of the biosensor to the cells expressing PSMA. In prospect, the detection of the biosensor will be tested on large spheroids and finally tested on in vivo model. [less ▲]

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See detailFermentation contrôlée des olives vertes de table.
Lamzira, Z.; Ghabour, N.; Thonart, Philippe ULg et al

Poster (2007, June 07)

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See detailSTUDY OF THE POSSIBILITY TO PRODUCE CELLULOSE MICROFIBRILS BY APPLYING SHEARING TREATMENTS TO CELLULOSE FIBERS
Jacquet, Nicolas ULg; Paquot, Michel ULg

Poster (2007, June 04)

Cellulose is a linear biopolymer composed of glucose residues linked by β 1-4 glucosidics bonds. These characteristics enable cellulose molecules to adopt an extended rod-like configuration. In the ... [more ▼]

Cellulose is a linear biopolymer composed of glucose residues linked by β 1-4 glucosidics bonds. These characteristics enable cellulose molecules to adopt an extended rod-like configuration. In the microfibrils, the multiple hydroxyl groups on the glucose residues hydrogen bond with each other, holding the chains firmly together and contributing to their high tensile strength. This study has shown the possibility to produce cellulose microfibrils by applying shearing treatment. Homogenisation and microfluidisation treatments permit to obtain microfibrils with 5 to 20 μm length and a 20 to 50 nm diameter. This study also shows that a tridimensional network, typical of a gel, appears in the treated samples [less ▲]

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See detailProspects for Nulling Interferometry from Antarctica
Coudé Du Foresto, V.; Absil, Olivier ULg; Barillot, M. et al

Poster (2007, June 01)

The high Antarctic plateau is a very unique environment whose main characteristics make it a premier site for high angular resolution, high dynamic range observations at infrared wavelengths. This is due ... [more ▼]

The high Antarctic plateau is a very unique environment whose main characteristics make it a premier site for high angular resolution, high dynamic range observations at infrared wavelengths. This is due to a combination of cold temperatures (low emissivity), dry air (infrared transparency), and a night time atmospheric turbulence which is concentrated in the first ~30m near the ground (which results in a large isoplanatic angle). Above that turbulent layer (a location that can be reached either by support structures or tethered balloons), the free air seeing is both exceptionally benign and slow. There, simulations show that a small dedicated interferometer (two 1m-class telescopes) equipped with a nuller instrument performs better than the same instrument behind 8m-class telescopes on a temperate site. It can characterize the distribution of dust emission around nearby main sequence stars, a necessary precursor science for Darwin and TPF-I. The nature of the site, intermediate between ground and space both in potential and technical challenge, adds particular relevance to the demonstration of nulling for a space mission. [less ▲]

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See detailFirst-Principles Study of High-Pressure Phase Transitions and Thermal Properties of Al2O3
Xu, Bin ULg; Dong, Jianjun

Poster (2007, June)

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See detailSugar beet leaves as source of green notes.
Gigot, Cédric ULg; Rabetafika, Holy-Nadia ULg; Fauconnier, Marie-Laure ULg et al

Poster (2007, June)

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See detailElaboration des olives vertes de table par voie biologique.
Ghabour, N.; Lamzira, Z.; Thonart, Philippe ULg et al

Poster (2007, June)

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See detailSalmonella spp on the pig meat through the cold chain in Belgium
Delhalle, Laurent ULg; Desadeleer, L.; Daube, Georges ULg

Poster (2007, June)

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See detailTowards the industrialization of traditional African fermented foods : a case study of fortified gari in Benin
Egountlety, M.; Adjakidje, A. S.; Segbedji, C. M. et al

Poster (2007, June)

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See detailDetection of Vibrio cholerae, V. parahaemolyticus and V. vulnificus in seafood using real time PCR.
Lemaire, Cédric; Darcy; China, Bernard et al

Poster (2007, June)

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See detailEtude comparative de la qualité de vie chez des sujets déments, MCI et vulnérables
Missotten, Pierre ULg; Di Notte, David; Squelard, Gilles ULg et al

Poster (2007, June)

L’étude de la qualité de vie (QdV) des personnes démentes suscite de plus en plus d’intérêt. Son évaluation soulève de nombreuses interrogations concernant son évolution au cours du processus démentiel ... [more ▼]

L’étude de la qualité de vie (QdV) des personnes démentes suscite de plus en plus d’intérêt. Son évaluation soulève de nombreuses interrogations concernant son évolution au cours du processus démentiel. Les facteurs explicatifs demeurent encore controversés. Dans le cadre de l’étude Qualidem (Paquay et al., 2004), nous avons évalué, sur une période de deux ans, la QdV de sujets déments (n=362) avec l’Alzheimer Disease Related Quality of Life-ADRQL (Rabins et al., 1999). Leur QdV initiale a été comparée à celle de sujets MCI (n=36) et vulnérables (n=124). Majoritairement féminines, les trois populations se composent de sujets âgés de 65 ans et plus résidant en institution ou au domicile. L’analyse des scores ADRQL globaux ( /100) montre que la QdV du groupe « déments » (65.89±17.06) est significativement inférieure à celle des groupes « MCI » (82.11±13.31) et « vulnérables » (75.64±20.44). MCI et vulnérables ne se différencient pas entre eux. Les scores ADRQL du groupe « déments » ne sont pas en relation avec le sexe, l’âge et le lieu de résidence (domicile/institution). Ils sont corrélés avec l’état cognitif (MMSE, CAMCOG), la dépendance (AIVJ, AVQ), les troubles psychologiques et comportementaux (CERAD/BRSD) et le stade évolutif (CDR-M). Le MMSE et la CERAD/BRSD ont un rôle explicateur (25.92%) sur la variance du critère « qualité de vie ». Le suivi du groupe déments (n=127) met en évidence une absence de modifications significatives de la QdV sur une période de 2 ans. Par contre, une dégradation de l’état clinique (MMSE, AIVJ, AVQ, CDR-M) s’observe. Celle-ci s’accompagne d’une augmentation du pouvoir explicateur des variables du tableau clinique (MMSE et CDR/M). En conclusion, la QdV du groupe « déments » n’est pas très nettement inférieure à celle des groupes « MCI » et « vulnérables ». De plus, elle demeure relativement stable sur une période de 2 ans malgré la dégradation du tableau clinique. Ces résultats confortent l’hypothèse selon laquelle l’évolution de la QdV ne serait pas uniquement déterminée par des paramètres neurodégénératifs mais également par des variables en lien avec les environnements physique et social au sein desquels la personne démente vit sa maladie. [less ▲]

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See detailEtude comparative de la qualité de vie de sujets déments en fonction de leur lieu de résidence et de leur état cognitif
Missotten, Pierre ULg; Di Notte, David; Thomas, Philippe et al

Poster (2007, June)

Classiquement, on affirme que la qualité de vie (QdV) des personnes démentes est meilleure au domicile qu’en institution. Toutefois, cette affirmation n’est guère étayée par des études comparatives ... [more ▼]

Classiquement, on affirme que la qualité de vie (QdV) des personnes démentes est meilleure au domicile qu’en institution. Toutefois, cette affirmation n’est guère étayée par des études comparatives utilisant un même instrument d’évaluation. En outre, il n’y a pas de consensus sur la nature des relations entre troubles cognitifs et QdV. Dans le cadre des études Qualidem (Paquay et al., 2004) et PIXEL (Thomas et al., 2006), la QdV de sujets déments et vulnérables a été évaluée au moyen de l’Alzheimer Disease Related Quality of Life (ADRQL; Rabins et al., 1999). Des analyses préliminaires ont démontré l’absence de différences significatives entre les populations Qualidem et PIXEL (variables âge, sexe, dépendance et QdV). L’échantillon total se compose de sujets majoritairement féminins âgés de 65 ans et plus. Tous les sujets déments ont un score MMSE compris entre 0 et 23 et sont répartis en 5 groupes: MMSE 0-3; 4-8; 9-13; 14-18 et 19-23. Tous les sujets vulnérables ont un score MMSE24 et se situent au stade évolutif normal de la Clinical Dementia Rating/Modified (CDR/M; Heymans et al., 1987). L’analyse des scores ADRQL ne met pas en évidence une diminution linéaire de la QdV en fonction de la sévérité des troubles cognitifs. Toutefois, le lieu de résidence influence le profil des scores. En institution (n déments=296; n vulnérables=12), ils diminuent au stade débutant de la démence (MMSE 19-23), se stabilisent dans les groupes MMSE 9-13 et 14-18 avant de s’abaisser fortement dans les groupe MMSE 4-8 et 0-3. Au domicile, (n déments=159; n vulnérables=14), on observe une phase de diminution dans les groupes MMSE 19-23 et 14-18 puis une phase de stabilisation dans les 3 derniers groupes. En conclusion, la QdV des sujets déments n’évolue pas linéairement en fonction de la dégradation de l’état cognitif. L’évolution différente des profils selon le lieu de résidence soulève la question de l’influence de la variable « informant » dans l’hétéro-évaluation de la QdV de personnes démentes. Au domicile, cet informant est l’aidant principal (soignant informel) alors qu’en institution, il s’agit d’un soignant formel. L’hypothèse de biais de surévaluation par le soignant formel et de sous-évaluation par le soignant informel peut donc être posée. [less ▲]

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See detailAcute peritonitis following rupture of a large rectal diverticulum in a dog
Saulnier-Troff, François-Guillaume; De Busscher, Virginie; Hamaide, Annick ULg

Poster (2007, June)

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See detailEffect of sexual genotype on the reproductive biology of the Nile tilapia, O.niloticus.
Rougeot, Carole ULg; Badiane, A. A.; Prignon, Christian ULg et al

Poster (2007, June)

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See detailAssessment of sea bass swimming activity and preferential space use in sea cages using acoustic telemetry and archival tags
Faucher, Karine ULg; Millot, Sandie ULg; Struski, Caroline et al

Poster (2007, June)

Swimming activity rhythms and levels as well as space occupation by sea bass, Dicentrarchus labrax, in a sea cage (6.5 x 6.5 x 8 m, ca. 25000 fish of 400 g stocked at 30 kg m-3) were recorded using ... [more ▼]

Swimming activity rhythms and levels as well as space occupation by sea bass, Dicentrarchus labrax, in a sea cage (6.5 x 6.5 x 8 m, ca. 25000 fish of 400 g stocked at 30 kg m-3) were recorded using acoustic telemetry (9 fish, IBDT Sonotronics) and archival tags (11, DSTs STAR ODDI). Acoustic tracking was realized over a 13 days period and successful for 3 individuals (one fix every 2 min on average). For archival tags, only 4 tags were retrieved and 3 had been recording swimming depth and temperature for 9 mo (Nov 05-June 06). Telemetry results showed an even day/night swimming activity levels in November with fish staying in close association with the bottom. Archival tags recordings revealed a preferential distribution in the water column between mid-water and bottom of the net cage in winter, over the whole water column in spring and near the surface zone in early-summer. In addition, on a 24 hrs period basis, sea bass presented a vertical movement rhythm: they swam closer to the surface during the day, specially around feeding events, and near the bottom at night. That vertical movement was pronounced during winter, faded during spring and almost disappeared in summer. [less ▲]

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See detailSugar beet : a source of biofuel an chemicals.
Destain, Jacqueline ULg; Gigot, Cédric ULg; Fauconnier, Marie-Laure ULg et al

Poster (2007, June)

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See detailMacromolecular engineering of poly(ε-caprolactone) based on macrocyclic units
Lecomte, Philippe ULg; Li, Haiying; Riva, Raphaël ULg et al

Poster (2007, May 24)

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See detailDIVA: a Data Analysis Software with Generalized-Cross Validation and Quality Control
Troupin, Charles ULg; Rixen, Michel; Sirjacobs, Damien ULg et al

Poster (2007, May 19)

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See detailMitochondrion plasticity evidenced by coupled comparisons of mitochondrial and cellular proteomes
Mathy, Grégory ULg

Poster (2007, May 16)

Mitochondria are key organelles in primary metabolism: they are the main source of energy production and the starting point of important biosynthetic pathways. Thus the control of the mitochondrial ... [more ▼]

Mitochondria are key organelles in primary metabolism: they are the main source of energy production and the starting point of important biosynthetic pathways. Thus the control of the mitochondrial function is a prerequisite for cellular survival in front of a wide spectrum of exogenous or endogenous stresses. By using the SILAC method, we have investigated the yeast mitochondrial and cellular proteome adaptation to the heterologous expression of a mitochondrial alternative oxidase (AOX). AOX is an energy dissipating enzyme that catalyses the re-oxidation of ubiquinol within the respiratory chain and compete with the Cytochrome pathway for electrons and thus, prevents proton pumping, and consequently leads to a decrease in ATP synthesis. [less ▲]

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See detailModels of irrigation system for Pursat basin planning
Ly, Sarann ULg; Someth, Paradis; Men, Nareth

Poster (2007, May 14)

The Mekong River and the Tonle Sap Great Lake in Cambodia have vast floodplain suitable for rice-based farming system. The Tonle Sap Basin, defined as the catchments of the Tonle Sap River at its ... [more ▼]

The Mekong River and the Tonle Sap Great Lake in Cambodia have vast floodplain suitable for rice-based farming system. The Tonle Sap Basin, defined as the catchments of the Tonle Sap River at its confluence with the Mekong River and other 11 main tributaries draining higher ground on all sides of the basin enclosing 44% of Cambodia’s land area. The Pursat River is one of tributaries of the Tonle Sap Great Lake. It originates at Cardamom Mountains and has very good potential for agricultural development. In this paper, we aim at applying three models of irrigation system for basin planning: Chinit (Dam-Reservoir Irrigation), West Baray (Reservoir Irrigation) and Batheay (Shallow Reservoir Irrigation) to Pursat Basin (one of sub-basin of the Tonle Sap Great Lake). Chinit Dam-Reservoir stores water supplied by one of tributaries of Tonle Sap Great Lake, the Chinit River, located in Kampong Thom Province. Spillway is built to pass up inundation and to release water to downstream part. The rest of water in the Dam-Reservoir is distributed to the paddy field by main canal, secondary canals and tertiary canals. This system can be put at the upstream part of the Pursat River, mountainous area. The West Baray Irrigation system is characterized by a reservoir combined with a dike system in the floodplain of the Tonle Sap Great Lake and an irrigation system situated in Siem Reap province. The reservoir is supplied by a tributary of the Tonle Sap Great Lake, the Siem Reap River, in rainy season. The water is distributed to irrigation area in dry season. A small dike system was constructed in the Tonle Sap floodplain for retarding and storing floodwater. The stored floodwater is used as an additional water source for dry season cultivation. This system can be placed at the central plain of the Pursat Basin. Batheay reservoir directly receives floodwater from the Mekong River. It functions as both a reservoir and a paddy field. In wet season, dike around the Batheay reservoir prevents floodwater from entering the reservoir. Rainy season rice is grown inside the reservoir. After harvesting, gates around the reservoir are opened to receive floodwater. The water is stored for cultivating dry season rice outside the reservoir. This system can be put at the downstream part of the Pursat River, floodplain area of Tonle Sap River. [less ▲]

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See detailSimulation of annual cycles of phytoplankton, zooplankton and nutrients to the South of Gran Canaria
Troupin, Charles ULg; Sangrà, Pablo; Arístegui, Javier

Poster (2007, May 11)

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See detailLe bm-573, un antagoniste original de récepteur au thromboxane a2, réduit le développement des lesions atheromateuses chez des souris deficientes en apolipoproteine e (apo e-/-)
Cherdon, Céline ULg; Rolin, Stéphanie; Hanson, Julien ULg et al

Poster (2007, May 10)

Afin d’examiner l'efficacité du BM-573 dans l'athérogenèse, des souris apo E-/- ont été traitées durant 10 semaines avec le BM573 (10mg/kg). Au cours de cette expérience, le traitement des animaux par le ... [more ▼]

Afin d’examiner l'efficacité du BM-573 dans l'athérogenèse, des souris apo E-/- ont été traitées durant 10 semaines avec le BM573 (10mg/kg). Au cours de cette expérience, le traitement des animaux par le BM-573, a eu pour effet de diminuer les lésions athéromateuses précoces de manière significative. Ces données ont été confirmées par des analyses histopathologiques et biochimiques. Ces résultats confirment que l'antagonisme sélectif des récepteurs TP associé à une inhibition de la thromboxane synthase réduit significativement les lésions athéromateuses chez les souris apoE-/-.. Le BM-573 est, par conséquent, un agent thérapeutique potentiel pour la prévention de l'athérosclérose [less ▲]

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See detailGlobal strategy for the development of estrogenic compounds detection screening test
Collodoro, Mike ULg; Makasinga, Elu; Lemaire, Pascale ULg et al

Poster (2007, May)

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See detailExploring the risk factors for Salmonella in the ten biggest Belgian pig slaughterhouses.
Delhalle, Laurent ULg; Desadeleer, L.; Bollaerts, K. et al

Poster (2007, May)

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See detailCharacterization of gels via solvent desorption measurements
Gommes, Cédric ULg; Noville, Francis; Pirard, Jean-Paul ULg

Poster (2007, May)

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See detailConception et synthèse d’outils pharmacologiques originaux pour l’étude des récepteurs au thromboxane
Moray, A. L.; Dogne, J. M.; Neven, P. et al

Poster (2007, May)

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See detailResolution recovery iterative reconstruction for human pinhole SPECT: a phantom study.
Seret, Alain ULg; Defrise, Michel; Andreyev, André et al

Poster (2007, May)

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See detailDaily quality control of activimeters: an absolute usefulness.
Seret, Alain ULg; Greffe, J.-L.

Poster (2007, May)

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See detailValorisation in bread of dietary fibres from by-products of the agro-industries.
Roiseux, Olivier; Sindic, Marianne ULg; Vanderbeke, E. et al

Poster (2007, May)

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See detailThe 1979-2005 Greenland ice sheet melt extent from passive microwave data using an improved version of the melt retrieval XPGR algorithm
Fettweis, Xavier ULg

Poster (2007, April 16)

Analysis of passive microwave satellite observations over the Greenland ice sheet reveals a significant increase in surface melt over the period 1979-2005. Since 1979, the total melt area was found to ... [more ▼]

Analysis of passive microwave satellite observations over the Greenland ice sheet reveals a significant increase in surface melt over the period 1979-2005. Since 1979, the total melt area was found to have increased +1.22 x 10ˆ7 kmˆ2. An improved version of the cross-polarized gradient ratio (XPGR) technique is used to identify the melt from the brightness temperatures. The improvements in the melt retrieval XPGR algorithm as well as the surface melt acceleration are discussed with results from a coupled atmosphere-snow regional climate model. From 1979 to 2005, the ablation period increases everywhere over the melt zone except in the regions where the model simulates an increased summer snowfall. Indeed, more snowfall in summer decreases the liquid water content of the snowpack, raises the albedo and therefore reduces the melt. Finally, this melt acceleration over the Greenland ice sheet is highly correlated with both Greenland and global warming suggesting a continuing surface melt increase in the future. [less ▲]

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See detailMorphology of the Io footprint
Bonfond, Bertrand ULg; Gérard, Jean-Claude ULg; Grodent, Denis ULg

Poster (2007, April 15)

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See detailCalcification and transparent exopolymer particles (TEP) production in batch cultures of Emiliania huxleyi exposed to different pCO2
De Bodt, Caroline; d'Hoop, Quentin; Harlay, Jérôme ULg et al

Poster (2007, April 15)

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP ... [more ▼]

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP accumulate until the end of the experiment and are enhanced after nutrient exhaustion. TEP contribute significantly to POC concentrations after the exponential growth phase. The very good correlation between TEP and calcite concentrations suggests that the calcification acts as a potential source of TEP in coccolithophore blooms. Finally, if calcite continues to decrease, calcification may be hampered in this species. [less ▲]

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See detailSCAR-MarBIN, the Antarctic marine biodiversity information network.
Danis, Bruno; et al.; Dauby, Patrick ULg et al

Poster (2007, April 12)

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See detailNK CELLS AND NKG2D LIGANDS IN HPV-ASSOCIATED CERVICAL CANCER
Jacobs, Nathalie ULg; Renoux, Virginie ULg; Dortu, Estelle ULg et al

Poster (2007, April 12)

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See detailInfluence of collar design on palatal implant primary stability
Bolland, Fabrice; Ronco, Vincent; LAMBERT, France ULg et al

Poster (2007, April)

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See detailAminoglycoside antibiotics and the sensory hair cells of inner ear and lateral line system in the Atlantic cod, Gadus morhua: effects on fish hearing threshold
Faucher, Karine ULg; Aas-Hansen, Øyvind; Damsgard, Borge et al

Poster (2007, April)

The aims of the present study were to investigate: a) the potential involvement of the fish lateral line system in hearing at 250 Hz and b) the possible regeneration of the inner ear hair cells in the ... [more ▼]

The aims of the present study were to investigate: a) the potential involvement of the fish lateral line system in hearing at 250 Hz and b) the possible regeneration of the inner ear hair cells in the Atlantic cod (Gadus morhua). The inner ear and lateral line system of the Atlantic cod were inactivated using ototoxic aminoglycoside antibiotics by injection (gentamicin) or bath (gentamicin and streptomycin), respectively. Hearing thresholds were measured in the fish using the Auditory Brainstem Response (ABR) technique at 10, respectively 17 - 20 days following antibiotic treatment. Results were compared between groups of individuals i) with inner ear damage, ii) with lateral line system altered, iii) placebo (immersion in fresh sea water) and iv) control. The sound stimuli applied were series of 2000 five-cycle (2-1-2) 250 Hz tone bursts delivered using a Brüel & Kjær (Nærum, Denmark) loudspeaker. ABRs were measured using a filter set at 30-3000 Hz, a gain of 100 000 and visual interpretation using the Bio-Logic AEP software. Sound stimulus level was reduced until threshold was attained. To check the tissue status of the inner ear and lateral line system, hair cells from sensory maculae were visualized using scanning electron microscope (SEM). Ten days after treatment, hearing thresholds of placebo and lateral line damaged cods did not differ from controls fish (p = 0.387 and p = 0.095, respectively), whereas the fish that received intravenous gentamicin injection had significantly elevated hearing thresholds compared to controls (p < 0.0001). SEM analyses further demonstrated that, 10 days following antibiotic treatment, cod inner ear hair cells were damaged, as well as lateral line hair cells and more particularly in the case of canal neuromasts. There was no hearing effect or microscopic tissue damage in untreated fish. From the 17th day after treatment, ABR measurements showed that some of the gentamicin treated fish had regained hearing thresholds comparable to the control cods (p = 0.535), and this was associated with an apparent regeneration of hair cells in the sensory maculae of their inner ear. This study indicates a capacity for inner ear hair cell regeneration in Atlantic cod, and illustrates the advantages of the ABR-method for investigation of fish hearing after intervention on the inner ear, and during the hair cell regeneration process. The present work further suggests that the lateral line system is not involved in cod hearing at 250 Hz. [less ▲]

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See detailDissolved inorganic carbon dynamics in the Gulf of Biscay (June 2006)
Suykens, K.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007, April)

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See detailEvaluation of a new commercial real time PCR for the detection of Aspergillus spp. in serum and respiratory samples
Hayette, Marie-Pierre ULg; Meex, Cécile ULg; Boreux, Raphaël ULg et al

Poster (2007, April)

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ ... [more ▼]

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ widely and comparisons are difficult to assess. The objective of the study is to compare a new commercial real-time PCR kit, affigene® Aspergillus tracer assay, with an in house nested PCR targeting 18S rRNA Aspergillus sp. gene. Methods. Twelve patients at risk for invasive aspergillosis were included in the study. They were classified to have possible (5 cases), probable (1 case) or proven (6 cases) invasive aspergillosis following E.O.R.T.C. criteria. Fifteen serum and respiratory paired samples were collected. The DNA extraction was performed by using the QIAmp DNA mini kit® (Qiagen, Germany). All samples were tested by both PCR assays and respiratory samples were cultured. Results. Respiratory samples. A. fumigatus, A. niger and A. flavus were isolated from 10/15 samples; both PCR methods were positive for these samples except one that was positive for affigene® and equivocal for the nested PCR. The real-time PCR assay reported cycle thresholds ranging from 25 to 38. Three of the five culture-negative samples were negative by both PCR methods; one of three was negative in affigene® assay and equivocal by nested PCR; the last sample was positive in affigene® assay and negative by nested PCR. Serum. Thirteen of fifteen blood samples were negative by both PCR methods. One sample was equivocal by nested PCR and was inhibited in affigene® assay despite a culture-positive paired respiratory sample. The last case was inhibited by the real-time PCR assay and negative by nested PCR. Nor the nested PCR, nor affigene® assay could detect any Aspergillus DNA in serum. In total, there was 93% of agreement between the two PCR assays. Conclusion. Both methods are in good agreement and can detect at least three different species of Aspergillus. However, the sensitivity of both assays does not permit the detection of Aspergillus DNA in serum. affigene® assay can easy replace the “in house” assay: it allows a fast and standardized detection of Aspergillus sp. DNA in respiratory samples without inconvenient due to the handling of PCR products. [less ▲]

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See detail. Study of Neuronal Preconditioning by ESR
Guelluy, Pierre-Henri ULg; Hoebeke, Maryse ULg

Poster (2007, April)

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See detailThe destabilising activities of tilted peptides
Lins, Laurence ULg; Brasseur, Robert ULg

Poster (2007, April)

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See detailInter-annual variability of the carbon dioxide oceanic sink south of Tasmania
Borges, Alberto ULg; Tilbrook, B.; Metzl, N. et al

Poster (2007, April)

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See detailDetermination of COF2 vertical distributions above Jungfraujoch by FTIR and multi-spectra fitting
Duchatelet, Pierre ULg; Mahieu, Emmanuel ULg; Demoulin, Philippe ULg et al

Poster (2007, April)

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. Photolysis of these compounds leads to release of chlorine atoms, while the fluorine is, in a first step, present in the form of ... [more ▼]

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. Photolysis of these compounds leads to release of chlorine atoms, while the fluorine is, in a first step, present in the form of carbonyl compounds like COClF and COF2. Their further photolysis liberates fluorine atoms, which are quickly converted to HF. Given its long life time, COF2 is the second stratospheric fluorine reservoir. The first COF2 vertical distributions were derived from occultation measurements performed by the ATMOS instrument during the SPACELAB-3 Space Shuttle mission in 1985. The Canadian FTIR spectrometer ACE-FTS, onboard the SCISAT-1 satellite, is the first instrument since the last ATMOS flight in 1994, to record COF2 vertical profiles from space. All these observations show that, at mean latitudes, COF2 concentration is maximum between 30 and 35 km. Several COF2 IR absorption lines located either in the so-called InSb (1-5 µm) and MCT (2-16 µm) spectral ranges can be used to determine its total column from ground-based FTIR observations. In this context, several studies concerning the evolution of COF2 total column above various stations were published during the nineties. At this time, no study concerning the inversion of COF2 vertical distributions from ground-based FTIR spectra has been published. This report deals with the feasibility of such inversions, using, simultaneously, via the SFIT-2 v3.91 algorithm, a multi-microwindows and a multi-spectra fitting procedure. The multi-spectra method consists of combining several FTIR observations, recorded during the same day, to increase the information content. A selection of microwindows in InSb and MCT ranges, a complete discussion about the data characterization (e.g. information content) and typical examples of COF2 retrieved profiles from high resolution solar spectra recorded with the University of Liège Jungfraujoch FTS will be revealed. [less ▲]

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See detailApproaches for retrieving abundances of methane isotopologues in the frame of the AGACC project from ground-based FTIR observations performed at the Jungfraujoch
Duchatelet, Pierre ULg; Mahieu, Emmanuel ULg; Demoulin, Philippe ULg et al

Poster (2007, April)

This work has been performed within the frame of AGACC(http://www.oma.be/AGACC/Home.html), a contribution to the Belgian Scientific Support for a Sustainable Development. The project intends to make an ... [more ▼]

This work has been performed within the frame of AGACC(http://www.oma.be/AGACC/Home.html), a contribution to the Belgian Scientific Support for a Sustainable Development. The project intends to make an advanced exploitation of existing ground-based remote-sensing measurements for a selection of atmospheric species that play an important role in the chemistry of the atmosphere and that have a direct or indirect impact on climate. Target species include -among others- lower tropospheric aerosols, H2O, HDO, CH4, HCN and CO. The instrumentation includes 3 types of spectrometers (FTIR, MAXDOAS and Brewer) and one CIMEL sun photometer. These instruments are operated at 3 different sites (Jungfraujoch, Ile de la Réunion and Uccle) and most of them are affiliated with the Network for the Detection of Atmospheric Composition Change (NDACC, formerly NDSC - http://www.ndacc.org), a group dedicated to performing high-quality long-term observations. This contribution will deal with the detection of the isotopologues of methane, a species released to the atmosphere by natural processes (e.g. wetlands, termites) as well as by anthropogenic activities (e.g. fossil fuel exploitation, rice agriculture, biomass burning, etc). Due to its high warming potential and its relatively long chemical lifetime, atmospheric methane is a major greenhouse gas. Methane also affects climate by influencing tropospheric ozone and stratospheric water. The cycle of methane is complex and a thoroughly study of the sources and sinks of its main isotopologue, as well as the other isotopic species, is necessary to characterize it. Isotopic ratios are also useful to differentiate between various sources of atmospheric methane. To study the vertical distribution of methane isotopologues from the high resolution FTIR spectra recorded by the University of Liège instrument at the International Scientific Station of the Jungfraujoch (ISSJ; 46.5°N, 8.0°E, 3580m a.s.l., Swiss Alps), we have selected several 13CH4 lines distributed in the so-called InSb (1-5 µm) and MCT (2-16 µm) spectral ranges. A set of four microwindows has also been selected for the study of CH3D. Using the SFIT-2 v3.91 algorithm, vertical column abundances as well as low-resolution vertical distributions have been retrieved, adjusted from an a priori profile defined on a 41 layers scheme and derived from ACE-FTS space observations. The information content and first preliminary retrieval results will be presented. [less ▲]

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See detailMeasured and modelled trends of stratopsheric Cly and Fy column amounts in the northern hemisphere
Ruhnke, Roland; Blumenstock, Thomas; Duchatelet, Pierre ULg et al

Poster (2007, April)

Reactive inorganic chlorine plays a crucial role in the stratospheric ozone depletion. To stabilize and enable a recovering of the stratospheric ozone layer, the Montreal protocol and its amendments and ... [more ▼]

Reactive inorganic chlorine plays a crucial role in the stratospheric ozone depletion. To stabilize and enable a recovering of the stratospheric ozone layer, the Montreal protocol and its amendments and adjustments have been progressively implemented to reduce or even stop the production and emission of important chlorinated source gases (CFCs, HCFCs, CCl4, CH3CCl3, and Halons). As these source gases are photolysed in the stratosphere into inorganic chlorine and fluorine, respectively, the turn over of the inorganic chlorine (HCl and ClONO2) and slowing down of fluorine (HF and COF2) reservoirs act as a verification of the effectiveness of these protocols. Here we present results of long-term measurements of the stratospheric column of HCl, ClONO2, and HF obtained at different stations in the northern hemisphere (Ny Alesund, Kiruna, Zugspitze, Jungfraujoch, Izana, all affiliated to the NDACC, Network for the Detection of Atmospheric Composition Change) within the PEP (Pole- Equator-Pole) network. These time series are interpreted with model calculations performed with a state of the art 2-D model and the 3-D CTM KASIMA with respect to the determination of the slowing down or turn over, respectively. In addition, trend parameters calculated using different approaches (e.g. linear trend, bootstrap-method) will be presented and intercompared. [less ▲]

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See detailDatation par luminescence des terres cuites architecturales : étude de briques de l’abbaye de St Philbert de Grandlieu (44, France)
Blain, Sophie ULg; Guibert, Pierre; Vieillevigne, Emmanuelle et al

Poster (2007, April)

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See detailProduction de pseudoparticules de norovirus humains et bovins et leur utilisation diagnostique
Mauroy, Axel ULg; Scipioni, Alexandra; Saegerman, Claude et al

Poster (2007, April)

Les norovirus sont des agents majeurs de gastroentérite humaine d’origine alimentaire et ceci à travers le monde entier. La contamination est habituellement oro-fécale. Les norovirus sont non enveloppés ... [more ▼]

Les norovirus sont des agents majeurs de gastroentérite humaine d’origine alimentaire et ceci à travers le monde entier. La contamination est habituellement oro-fécale. Les norovirus sont non enveloppés et ont un génome composé d’ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Au sein de ce génome, trois cadres ouverts de lecture (ORFs) sont décrits. L’ORF 1 code pour une polyprotéine qui sera par la suite clivée pour donner les différentes protéines non-structurales. L’ORF 2 encode pour l’unique protéine de capside. Une protéine structurale mineure dont le rôle est encore peu caractérisé est encodée par l’ORF 3. Ces virus sont très résistants dans l’environnement et une infection peut survenir même avec une très faible dose infectieuse. Appartenant à la famille des Caliciviridae, le genre norovirus est composé de cinq génogroupes (G) et contient également des virus infectant les animaux (bovins, porcins, murins). L’impact des norovirus animaux et plus particulièrement des norovirus bovins peut être envisagé à travers un possible caractère zoonotique de ces virus ; ainsi ils pourraient par exemple être transmis à l’homme via des eaux d’effluent contaminées. L’étude des norovirus humains et bovins est encore à l’heure actuelle entravée par l’absence d’un système de culture cellulaire. Cependant différents systèmes d’expression protéique ont été utilisés afin d’exprimer la protéine de capside, celle-ci s’assemblant spontanément avec d’autres pour reformer des pseudoparticules virales ou virus-like particles (VLPs). Ces VLPs sont morphologiquement et antigéniquement semblables aux virus natifs. Le but de ce travail était dans un premier temps d’obtenir des VLPs de souches humaines et bovines. Les gènes codant pour la protéine de capside d’une souche humaine (H384) et d’une souche bovine (B309) ont été amplifiés par RT-PCR à partir d’échantillons de matières fécales collectés dans des laboratoires de diagnostic humain et vétérinaire. Ces gènes ont été séquencés et comparés grâce à des outils informatiques (Basic Local Alignment Search Tool, BLAST) à la banque de séquences déjà disponibles sur le site du NCBI. Nous avons pu déterminer que la séquence de capside de la souche H384 était proche de celle de la souche HuNV/Altenkirchen 140/01/DE, une souche de GII et de génotype 4 tandis que la séquence de la souche B309 était proche de celles de GIII et de génotpe 2 (groupe Newbury2). Des plasmides contenant les séquences des ORFs 2 de deux souches de norovirus humains de référence, la souche de Norwalk (GI) et celle de Hawaii (GII), ont été aimablement fournies par le Docteur Jan Vinjé (CDC, Atlanta, USA). Le système d’expression protéique baculovirus (Invitrogen) a été utilisé pour obtenir des VLPs. Brièvement, des cellules d’insecte des lignées Sf9 et H5 ont été infectées avec des baculovirus recombinants pour la protéine de capside des norovirus. Les VLPs ont été purifiées à partir des surnageants par ultracentrifugation sur un coussin de sucrose à 30% suivie d’une ultracentrifugation en gradient de chlorure de césium. Ce gradient a été fractionné et les différentes fractions ont été analysées pour la présence de la protéine de capside des norovirus par SDS-PAGE. Pour vérifier que les fractions positives contenaient bien des VLPs, les surnageants ont été observés en microscopie électronique. Nous avons ainsi obtenu des VLPs de norovirus de trois des cinq génogroupes décrits (GI, II et III). Ces VLPs ont été utilisées pour immuniser des souris afin de produire des anticorps monoclonaux et pour immuniser des lapins afin d’obtenir des anticorps polyclonaux. Nous avons pu aussi tester une banque de sérums bovins par ELISA pour la présence d’anticorps dirigés contre les souches de norovirus de génogroupe III et de génotype 2, les plus fréquemment mises en évidence au sein de notre banque de matières fécales bovines. [less ▲]

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See detailEvaluation of the cytotoxicity of cyclodextrins on endothelial cells using MTT assay
Castagne, Delphine ULg; Belhadj Salem, Leila; Delattre, Luc ULg et al

Poster (2007, April)

Detailed reference viewed: 24 (1 ULg)
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See detailOverview on the ADONIS Project: Accurate Diagnosis of prostate cancer using Optoacoustic detection of biologically functionalized gold Nanoparticles - A new Integrated Biosensor System
Fleron, Maximilien ULg; Schol, Daureen ULg; Greisch, Jean-François et al

Poster (2007, March 21)

Overview on the ADONIS Project: Accurate Diagnosis of prostate cancer using Optoacoustic detection of biologically functionalized gold Nanoparticles - A new Integrated Biosensor System

Detailed reference viewed: 16 (1 ULg)