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See detailChronologie de l’architecture médiévale et datation par luminescence : étude de l’église St-Irénée de Lyon (France)
Bouvier, Armel; Guibert, Pierre; Blain, Sophie ULg et al

Poster (2010, November)

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See detailA percepção da expressão físico-motora por professors do ensino basico
do Couto, Joao; Cloes, Marc ULg; Sequeira, Pedro

Poster (2010, November)

This poster proposes the findings of a study aiming to identify the representations of elementary school classroom teachers about physical education.

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See detailSynthesis of core cross-linked micelles for the development of new drug delivery systems
Cajot, Sébastien ULg; Danhier, F.; Lautram, N. et al

Poster (2010, October 29)

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See detailKashin-Beck Disease: evaluation of mineral intake in young Tibetan children from endemic areas
DERMIENCE, Michael ULg; Maesen, Philippe ULg; Mathieu, Françoise et al

Poster (2010, October 26)

Kashin-Beck disease is an endemic and chronic osteochondropathy. This disease principally occurs in the Tibet Autonomous Region and in several provinces of the People’s Republic of China. Although many ... [more ▼]

Kashin-Beck disease is an endemic and chronic osteochondropathy. This disease principally occurs in the Tibet Autonomous Region and in several provinces of the People’s Republic of China. Although many studies have already been conducted and many others are still underway, its ethiology remains unknown. A multifactorial hypothesis has been proposed: selenium deficiency, high concentration of organic matters in drinking water (fulvic acids) and mycotoxin poisoning by fungi infecting cereals. This original study aimed to measure the mineral contents of the food most often consumed in severe endemic regions and then to evaluate the daily intake of minerals in young Tibetan children from endemic areas. The mineral elements were selected in relation to their implications in bone metabolism. A sampling campaign split up into two time periods (winter and spring) was carried out. Ten families from two distinct regions were selected based on three criteria: they live in endemic areas; they include a 3 to 5 year-old child; this child has a KBD brother or sister. At the same time, a nutritional survey was made by the means of a prospective questionnaire in order to list the 24h food intake of the 3 to 5 year-old child. This survey highlighted the extremely monotonous cereal-based Tibetan diet. An analytical method for the minerals was developed as follows: mineralization of samples performed by microwave-assisted wet process; mineralized solutions measured by several atomic absorption or emission spectrometric methods and molecular absorption spectrometric methods. The analytical method was validated by mean of certified reference materials. Mean food contents were calculated and compared to food composition reference tables. High iron contents and selenium deficiencies were highlighted in several foods. Daily intakes were estimated combining mineral measurements and nutritional survey results. These were compared to dietary reference intakes from reference tables. This estimation reveals some crucial points: we confirm a marked deficiency in calcium; Ca/P ratios are always low; iron and copper intakes are excessive; zinc is the most probably deficient; while selenium could be deficient; manganese intakes often exceed toxicity thresholds. Nevertheless, this study encounters some limits. The bioavailability of minerals is a critical point that deserves further investigations. Moreover, a larger study over a longer term covering both endemic and non-endemic regions is required for definite conclusions to be reached. [less ▲]

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See detailAdaptive reflective processes using the IMS-LD specification
Verpoorten, Dominique ULg; Kelle, S

Poster (2010, October 26)

The research work presented in this poster coins a new range of reflection support tools called "REFLECTION AMPLIFIERS", viz. deliberate and well-considered prompting approaches that offer learners ... [more ▼]

The research work presented in this poster coins a new range of reflection support tools called "REFLECTION AMPLIFIERS", viz. deliberate and well-considered prompting approaches that offer learners structured opportunities to examine and evaluate their own learning. [less ▲]

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See detailInterest of routine dosage of meropenem in difficult to treat infections
Frippiat, Frédéric ULg; Bensahi, Ilham; Denooz, Raphael ULg et al

Poster (2010, October 23)

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See detailImpact of grazing by amphipods on the epiphytic cover of the Posidonia oceanica leaves: an in situ experiment.
Michel, Loïc ULg; Dupont, Alessandra; Gobert, Sylvie ULg et al

Poster (2010, October 22)

It is now established that several species of amphipods associated to Mediterranean Posidonia oceanica meadows consume the macro-epiphytes present on the leaves of the seagrass. Moreover, under controlled ... [more ▼]

It is now established that several species of amphipods associated to Mediterranean Posidonia oceanica meadows consume the macro-epiphytes present on the leaves of the seagrass. Moreover, under controlled in vitro conditions, three amphipod taxa (Apherusa chiereghinii,Dexamine spiniventris and Gammarus spp.) are able to drastically deplete the biomass of erected algae, thus influencing the epiphytic cover in both a quantitative and a qualitative way. Here, we tried to assess whether this strong and complex trophic interaction was realized in the field. We designed an in situ experiment that used microcosms placed directly in the meadow, at a depth of 10m, to estimate the impact of grazing by the aforementioned amphipod taxa on the dynamics of the epiphytic cover. Both Gammarus spp. and Dexamine spiniventris caused a significant decrease of the biomass of erected algae and erected animals (bryozoans and hydrozoans). Impact of grazing by Apherusa chiereghinii on these two epiphytic groups was less important, although strong but marginally non-significant (0,1>p>0,05) trends to lower biomasses were present. None of the considered taxa seemed to consume encrusting macro-epiphytes. In addition, assimilation of epiphyte-derived carbon and nitrogen by grazers was monitored using stable isotopes (13C and 15N), and epiphyte elemental content (C & N) was measured. Our results shed light on trophic interactions between the amphipods from Posidonia oceanica meadows and the seagrass epiphytic cover, and thus enhance our understanding of the role of these grazers in the functioning of the meadow as an ecosystem. [less ▲]

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See detailSPATIO-TEMPORAL LOCALIZATION OF BETA TUBULIN III IN THE ORGAN OF CORTI AND IN THE SPIRAL GANGLIA BETWEEN THE EMBRYONIC DAY (E18) AND THE POST-NATAL DAY (P25) IN RAT.
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Cloes, Marie ULg et al

Poster (2010, October 22)

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, at least two types of ... [more ▼]

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, at least two types of sensory cells can be identified in the OC: inner and outer hair cells. The structure of this organ is well reported in adult but its development is still little-known. By using confocal microscopy, we studied the spatial-temporal distribution of beta tubulin III during the differentiation of the OC in rat from the embryonic day 18 (E18) to the postnatal day (P25). The beta tubulin III is typical for neural cells in the OC. We observed that beta III tubulin is present in the extensions innerving the row of inner hair cells at E18. At E19, the extensions innerving the inner hair cells and the two first rows of outer hair cells were immunolabelled. From E21 to P25, all of hair cells were connected to the spiral ganglion. In the latter, the intensity of immunolabelling decreased between E18 to P25 and the labelling only concerned some cells. These results reveal that beta III tubulin appears before birth in the nervous extensions connecting the sensory cells of the OC according to a modiolar-to-striolar gradient. In the spiral ganglia, the labelling progressively decreases during its development. [less ▲]

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See detailThe role of meiofauna in energy transfer in a Mediterranean seagrass bed (Calvi, Corsica)
MASCART, THIBAUD; Lepoint, Gilles ULg; Borges, Alberto ULg et al

Poster (2010, October 22)

Meiofaunal communities of the endemic Mediterranean seagrass, Posidonia oceanica, were sampled in five different habitats characterised by different degradation level of macrophytodetritus. In term of ... [more ▼]

Meiofaunal communities of the endemic Mediterranean seagrass, Posidonia oceanica, were sampled in five different habitats characterised by different degradation level of macrophytodetritus. In term of abundance, harpacticoid copepods represent half of the community followed by nematodes and polychaetes. Two meiofauna communities were distinguished: (1) a benthic community of meiofauna, living in the sediment or on highly fragmented macrophytodetritus, and (2) a foliar, epiphytal community associated with seagrass leaves and low fragmented macrophytodetritus leaves. They differed significantly in their harpacticoid copepod family composition. The benthic community consisted mainly of families like Tisbidae and Miraciidae, while the epiphytal community was dominated by families like Thalestridae and Laophontidae. These differences in composition may also imply a differential functional diversity. Trophic biomarkers (stable isotopes, fatty acids) were used to identify the major sources of organic matter contributing to the copepods diet and hence to gain insight in the overall carbon flux. Harpacticoid copepods showed preferences to feed upon the epiphytal biofilm community composed of bacteria, diatoms, fungi and microalgae. Copepods used the seagrass and detritus material merely as substrate, but were attracted to the biofilm rather than the plant material which is rich in structural carbohydrates difficult to assimilate by animals (i.e. lignin, cellulose, ...). Since harpacticoid copepods showed to use different sources of carbon, unravelling the contribution of each of them and the role of the degradation level of the detritus for food selectivity is the next step forward. [less ▲]

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See detailLa sectorisation des cours d'eau wallons
Burton, Christophe ULg; Henrotay, Frédéric ULg; Borensztein, Aurélie ULg et al

Poster (2010, October 20)

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See detailUtilisation de la CNOSW en tant que carte d'occupation du sol dans le modèle EPICgrid
Sohier, Catherine ULg; Degre, Aurore ULg

Poster (2010, October 20)

Le modèle EPICgrid est un modèle hydrologique distribué de résolution 1km² couvrant toute la Wallonie. Il a été développé par Gx-ABT afin d’évaluer les flux d’eau, de nutriments et de sédiments vers les ... [more ▼]

Le modèle EPICgrid est un modèle hydrologique distribué de résolution 1km² couvrant toute la Wallonie. Il a été développé par Gx-ABT afin d’évaluer les flux d’eau, de nutriments et de sédiments vers les eaux de surface et vers les eaux souterraines. Il permet, entre autres, de tester l’impact de scénarios de gestion des nutriments sur la qualité des eaux en Région wallonne ainsi que des rapportages à différentes échelles dont les masses d’eau de surface. Le modèle requiert parmi ses données d’entrée une cartographie de l’occupation du sol. Dans un premier temps, la carte d'occupation des sols utilisée était issue du projet CARHY (Laime et Dautrebande, 1995) et reposait sur l'analyse d'images satellitaires Landsat pour refléter l’occupation du sol des années '90. En 2009, la CNOSW a été implémentée dans le modèle pour affiner spatialement et mettre à jour cette donnée en vue de simulations hydrologiques prospectives. Pour ce faire, les différentes classes de la CNOSW (niveau 5) ont été complétées au niveau des zones non cadastrées afin d’obtenir une couverture continue, hydrologiquement valide, de toute la Wallonie. Les classes ont ensuite été regroupées en classes hydrologiques d’occupation du sol (7 classes). La comparaison des cartes d’occupation du sol CARHY et issue de la CNOSW montre une répartition en classes similaire au niveau régional. Néanmoins, au niveau local, les différences marquées peuvent être constatées. Les zones urbaines sont également mieux représentées par la CNOSW. L’introduction de la CNOSW dans le modèle EPICgrid en apportant une spatialisation plus précise des occupations du sol a permis d’affiner les résultats du modèle et d’ouvrir de nouvelles pistes de développement telles que la représentation dans le modèle hydrologique du fonctionnement des bandes enherbées riveraines et inter-parcellaires. [less ▲]

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See detailProjet GISER - Elaboration d'un module de calcul des écoulements par la méthode SCS
Colard, François ULg; Mokadem, Abdel Ilah; Cordonnier, Hélène et al

Poster (2010, October 20)

Le projet GISER (gestion intégrée sol érosion ruissellement) consiste en différents axes de travail. Le poster présente un des outils opérationnels de calcul du ruissellement et des rendements en ... [more ▼]

Le projet GISER (gestion intégrée sol érosion ruissellement) consiste en différents axes de travail. Le poster présente un des outils opérationnels de calcul du ruissellement et des rendements en sédiments qui a été développé sur base des informations cartographiques existantes. Il utilise la carte d'occupation des sols mais également le MNT, la carte des sols, et les produits du projet ERRUISSOL. [less ▲]

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See detailBlock of SK channels by the sigma agonist 1,3-di-o-tolyl-guanidine: evidence for a novel site of action for SK blockers
Dilly, Sébastien ULg; Lamy, Cédric; Snyders, Dirk et al

Poster (2010, October 16)

Among ion channels involved in the control of neuronal activity, small conductance calcium-activated potassium channels (SK) represent an interesting therapeutic target. Indeed, they underlie medium ... [more ▼]

Among ion channels involved in the control of neuronal activity, small conductance calcium-activated potassium channels (SK) represent an interesting therapeutic target. Indeed, they underlie medium duration afterhyperpolarizations (mAHPs) in many types of neurons, thus inhibiting cell excitability. Three subtypes of SK subunits, SK1, SK2 and SK3, have been cloned and are expressed differentially within the central nervous system (CNS). Blocking SK channels might be beneficial in the treatment of several CNS disorders such as depression (SK3), Parkinson’s disease (SK3) and cognitive disorders (SK2). So far, the prototypical blocker of SK channels is apamin, an octadecapeptide from bee venom. We have recently shown that apamin blocks SK channels by binding to a site distinct from that used by classical pore blockers such as tetraethylammonium (TEA) (Lamy et al. J. Biol. Chem. 2010, 285, 27067-77). We have also demonstrated that the nonpeptide blocker N-methyl-laudanosine (NML) (Scuvée-Moreau et al. J. Pharmacol. Exp. Ther. 2002, 302, 1176-83) competes for the binding site of the toxin. Further, our research team has recently shown that the sigma agonist 1,3-di-o-tolyl-guanidine (DTG) directly blocks SK currents in a voltage-independent manner (Lamy et al. Eur. J. Pharmacol. 2010, 641, 23-8). We have combined patch clamp experiments on cell lines with molecular modelling and mutagenesis, to try to identify the site where DTG blocks. DTG was found to be equipotent on wild-type (WT) and apamin-insensitive (e.g. SK2H337N) channels. Moreover, mutated channels with increased sensitivity to TEA (SK3V520F: mean IC50 of TEA: 0.34 mM versus 11 mM for WT channels) were blocked by DTG with the same potency as WT channels. Thus, DTG does not seem to share the site of either apamin or TEA. Modelling data were in agreement with this possibility because of the identification of various potential binding sites. Although preliminary, these results suggest the existence of yet another binding site in the outer pore region of SK channels. [less ▲]

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See detailTowards the synthesis of mannose derivatives of natural phenolic compounds
Sainvitu, Pauline ULg; Nott, Katherine ULg; Richard, Gaetan ULg et al

Poster (2010, October 14)

The aim of this project is to graft a sugar moiety onto polyfunctional natural phenolic compounds. This should enhance their water solubility. The choice of an adequate sugar such as mannose could provide ... [more ▼]

The aim of this project is to graft a sugar moiety onto polyfunctional natural phenolic compounds. This should enhance their water solubility. The choice of an adequate sugar such as mannose could provide cellular recognition. The synthesis route was first tested on cinnamyl alcohol which is structurally close to the base pattern of natural phenolic compounds. <br />Two compounds are tested to catalyse the glycosilation between cinnamyl alcohol and D-mannose. The first one is an enzyme, the -glucosidase from almond, and the second one is a mineral acid catalyst immobilized on silica. Results show that -glucosidase is able to synthetize cinnamyl mannoside from mannose and cinnamyl alcohol. Furthermore, enzyme-catalyzed route lead to only one product and is so more specific than the chemical route where several products are observed. The obtaining of one product with a unique structure is interesting for the fundamental study of structure-function relationships (Interaction of the product with model membranes by Isothermal Titration Calorymetry and with the Langmuir Trough technique). In a future work, the reaction will be tested with more complex molecules (for example coniferyl alcohol). [less ▲]

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See detailComparative toxicity of Deverra scoparia essential oil and blends of its major constituents against Tetranychus urticae Koch
Attia, Sabrine; Grissa, Kaouthar Lebdi; Lognay, Georges ULg et al

Poster (2010, October 14)

The phytophagous two-spotted spider mite Tetranychus urticae Koch is a ubiquitous species, present worlwide on a large variety of plant families. Since T. urticae resistance spread rapidly, biological ... [more ▼]

The phytophagous two-spotted spider mite Tetranychus urticae Koch is a ubiquitous species, present worlwide on a large variety of plant families. Since T. urticae resistance spread rapidly, biological control tactics are crucial to manage spider mite populations. In this respect, plant-derived essential oil products are a good alternative as they are, in general, considered as minimum-risk pesticides. In that context, laboratory experiments were conducted to assess the toxicity of essential oil of Deverra scoparia endemic plant to North Africa on females of the two spotted spider mite T. urticae (Koch). Essential oil was distillated from fresh leaves from D. scoparia and was sprayed on groups of adults females. The susceptibility of these females to D. scoparia essential oil was tested. A series of dilutions were used to bracket the dose-response range. Tests proved that female mortality increased with essential oil concentration with DL50 and DL90 values of 1.79 mg/l and 3.16 mg/l, respectively. The analysis of D. scoparia essential oil using GC-MS revealed presence of 10 major constituents: alpha-thujene, alpha-pinene, sabinene, myrcene, 3-carene, ocimene, terpinene-4-ol, pulegone, eugenol and beta-eudesmol. For a comprehensive evaluation of the potential of D. scoparia essential oil as acaricidal, individual blends activity of these constituents were tested against T. urticae female. Toxicity of blends of different components indicated significant differences among the active and inactive components, with the presence of all constituents necessary to have toxicity near to that of whole D. scoparia oil. The results showed that natural oil of D. scoparia and some of its constituents have potential for development as botanical acaricide, at least against T. urticae. [less ▲]

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See detailUtilisation du jus de datte comme milieu d’immersion pour la déshydratation osmotique des graines de grenade
Bchir, Brahim ULg; Besbes, Souhail; Karoui, Romdhane et al

Poster (2010, October 14)

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See detailGeneration of camelid single-domain antibody fragments raised against proteins containing polyglutamine expansions
Pain, Coralie ULg; Scarafone, Natacha; Jaspar, Aurélie et al

Poster (2010, October 14)

Nine progressive neurodegenerative diseases are associated with the expansion of a polyglutamine (polyQ) tract above a threshold size (~ 35-45 residues) into nine different proteins [1]. These proteins ... [more ▼]

Nine progressive neurodegenerative diseases are associated with the expansion of a polyglutamine (polyQ) tract above a threshold size (~ 35-45 residues) into nine different proteins [1]. These proteins with expanded polyQ repeats have been found to form intranuclear amyloid-like aggregates, and the formation of these aggregates could play an important role in the pathogenesis [2-4]. The polyQ expansion is the only common feature among the proteins involved, suggesting it may be responsible for the aggregation phenomenon. Understanding the molecular mechanism by which the polyQ expansions promote aggregation is therefore crucial for the development of therapeutic strategies. The nine proteins associated with polyQ diseases are difficult to express recombinantly due to their big size and/or their insoluble character. In order to get further insights into the mechanism by which polyQ tracts promote aggregation, we have therefore decided to insert polyQ sequences into a well studied protein, the b-lactamase BlaP from B. licheniformis [5-6]. We have created chimeras containing 23, 30, 55, and 79 glutamines and we have investigated the effects of the insertions on the activity, the structure, the stability of BlaP as well as on its aggregating properties. Preliminary results indicate that BlaP is a good framework to study the molecular mechanism of aggregation associated with expanded polyglutamine tracts. On another hand, our previous work on the amyloidogenic variants of human lysozyme has shown that camelid single domain antibody fragments are very powerful structural probes to understand, at the molecular level, the mechanism of amyloid fibril formation [7]. Moreover, a recent study has suggested that expanded polyQ strectches adopt multiple conformations in solution that can be readily distinguished by monoclonal antibodies [8]. Altogether these results have encouraged us to generate VHHs against our different chimeras and we present here our preliminary results. References [1] Orr and Zoghbi (2007) Annu Rev Neurosci 30, 575-621. [2] DiFiglia et al. (1997) Science 277, 1990-1993. [3] Paulson HL (2000) Brain Pathol 10, 293-299. [4] Sanchez I. et al. (2003) Nature 421, 373-379. [5] Scarafone N. (2008) Mémoire de DEA en Sciences. Université de Liège. [6] Pain C. (2009) Mémoire de Master en Biochimie. Université de Liège. [7] Dumoulin et al. (2003) Nature 424, 783-788. [8] Legleiter J. et al. (2009) J Biol Chem 284, 21647-21648. [less ▲]

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See detailNew insights into the transformation of carbohydrates into platform chemicals
Richel, Aurore ULg; Wathelet, Jean-Paul ULg; Paquot, Michel ULg

Poster (2010, October 14)

The synthesis of furanic compounds from carbohydrates has become exciting in chemistry and in catalysis studies, because it represents one of the major routes for achieving sustainable energy supply and ... [more ▼]

The synthesis of furanic compounds from carbohydrates has become exciting in chemistry and in catalysis studies, because it represents one of the major routes for achieving sustainable energy supply and chemicals production.1 In particular, 5-hydroxymethylfurfural (5-HMF) and 2-furfural (2-F) occupy a pivotal place and are recognised as versatile and multi-functional starting points for the synthesis of a panel of precursors. Microwaves have readily appeared as a powerfull technique for accelerating reaction rates and increasing selectivities in the field of carbohydrate chemistry.3 In particular, their involvment in combination with heterogeneous catalysts have opened new vistas in the production of bio-oxygenated platform chemicals. The state of the art and main outlook of this strategy are reported here. [less ▲]

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See detailSynthèse assistée par Micro-ondes de dérivés d'acides uroniques
Laurent, Pascal ULg; Richel, Aurore ULg; Wathelet, Bernard ULg et al

Poster (2010, October 14)

At the moment, biorefining is increasingly seen as a promising alternative to petrochemical sector since it targets both the replacement of part of petroleum as a source of energy and the development of ... [more ▼]

At the moment, biorefining is increasingly seen as a promising alternative to petrochemical sector since it targets both the replacement of part of petroleum as a source of energy and the development of chemicals from the biomass, such as detergents, phytopharmaceutics, solvents, plastics, etc. The valorisation of carbohydrates arising from the hydrolysis of renewable feedstocks is therefore an area of outmost interest. In this context, uronic acids such as glucuronic acid (GlcA) or galacturonic acid (GalA) derived from widely available raw material such as hemicellulose or pectins represent important biocompatible and bioresorbable starting material. The quest of highly effective, environmentally friendly and straightforward chemical strategies to transform totally O-unprotected uronic acids into high valuable materials remains actually a particularly challenging task. A new strategy enabling the quantitative “one-pot” production of water-soluble monosubstituted D-glucofuranosidurono-6,3-lactones from unprotected D-glucuronic acid (D-GlcA) involving microwaves and an inexpensive siliceous-based promoter will be described. The use of a heterogeneous acid catalytic systems consisting of sulfuric acid impregnated onto silica (H2SO4/SiG60) or onto carbon (H2SO4/C), offers a green alternative to unrecyclable liquid sulfuric acid and permit the developpment of a truly eco-friendly green process, as these supported acids were readily separated from liquid products, without neutralization, by decantation or filtration, thus minimizing energy consumption and wastes. Faced with environmental concerns, this solventless methodology offers attractive features, including short reaction times, high yields and easy set-up and workup. [less ▲]

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See detailVolatile collection of cadaveric compounds
Dekeirsschieter, Jessica ULg; Verheggen, François ULg; Haubruge, Eric ULg

Poster (2010, October 14)

Thanatochemistry, also named ''chemistry of death'', is poorly studied and the available information regarding the volatile organic compounds (cadaveric VOCs) released after death are rather limited ... [more ▼]

Thanatochemistry, also named ''chemistry of death'', is poorly studied and the available information regarding the volatile organic compounds (cadaveric VOCs) released after death are rather limited. Thanks to the use of analytical chemistry methods ((TDS)GC-MS, GCxGC-TOF-MS), the olfactive signature of a dead body may be studied during the decomposition process. Different volatile collection techniques are used to study the smell of death. There are passive sampling techniques (Radiello® diffusive sampler) and dynamic sampling technique (pump device). The smell of death is constituted by a blend of hundreds of volatile organic compounds which change during the decay process. Main products detected are sulphur compounds such as sulphur dioxide, dimethyldisulfide and dimethyltrisulfide; alcools (1-butanol, 3-methyl-1-butanol), acids (butanoic acid, 2-methylbutanoic acid, 3-methylbutanoic acid and propanoic acid). Many cyclic hydrocarbons were detected: indole, phenol, p-cresol and piperidin-2-one are some examples. The aldehydes are also present, overall butanal, hexanal, heptanal and nonanal. We however found no trace of cadaverine or putrescine. Our results may have potential implication in a better understanding of the olfactive signature of a human or animal cadaveric corpse. Especially in the field of forensic entomology, these chemical compounds may have an attractive role on the necrophagous insect behaviour. Further studies based on the relationships that may exist between cadaveric VOCs and necrophagous insects are currently conducted at the Department of functional and evolutionary Entomology (GxABT, Ulg). [less ▲]

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See detailLipase synthesis of fatty acid sugar esters in pure ionic liquids
Galonde, Nadine ULg

Poster (2010, October 14)

Sugar fatty acid esters are broadly consumed in many commercial applications and their synthesis gained a considerable interest since they are biodegradable, tasteless, odorless, non-toxic and non ... [more ▼]

Sugar fatty acid esters are broadly consumed in many commercial applications and their synthesis gained a considerable interest since they are biodegradable, tasteless, odorless, non-toxic and non-irritant. Ionic liquids are organic salts entirely comprised of ions with a melting point below 100°C. This new category of solvent may allow the enzymatic synthesis of sugar fatty acid ester to go toward a “greener” process. Within the purpose of using “green” systems for synthesis of glycosylated compounds, studies on the use of ionic liquids to obtain fatty acid sugar esters with carbohydrates coming from lignocellulosic material as substrates was carried out. The enzymatic synthesis of mannose myristate ionic liquids as enzymatic medium and the yield obtained will be presented in our poster. [less ▲]

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See detailA method for the production of prebiotic preparations containing isomaltooligosaccharides and gluconic acid.
Goffin, Dorothée ULg; Blecker, Christophe ULg; Paquot, Michel ULg

Poster (2010, October 14)

Isomaltooligosaccharides (IMOs) are non-digestible oligosaccharides, considered as prebiotics and therefore aim to selectively feed probiotics indigenous to the human colon. IMOs consists of glucose ... [more ▼]

Isomaltooligosaccharides (IMOs) are non-digestible oligosaccharides, considered as prebiotics and therefore aim to selectively feed probiotics indigenous to the human colon. IMOs consists of glucose monomers linked by at least one α-1-6, or in a lower proportion α -1-3 (nigerose family) or α -1-2 (kojibiose family) glucosidic linkages. In our case they are produced from either corn, tapioca, or rice hydrolyzed starch. The enzymatic reaction is achieved using an Aspergillus niger transglucosidase (EC 2.4.1.24). It results in a very complex mixture with molecules characterized at the same time by their DP value (from 2 to ~15), linkages types (α-1-2, 3 or 6) and the proportion and position of each type of linkage (only α -1-6 or combined types). However, the reaction only permits to reach yields between 50-75 % in IMOs. Impurities are composed of residual maltooligosaccharides (glucose with exclusively α -1-4 linkages) from the starting vegetal material and glucose released during the transglucosylation step. These digestible saccharides are deleterious for the prebiotic preparation. Therefore, these compounds must be eliminated from the medium or converted in prebiotic species. Residual maltooligosaccharides are thus specifically hydrolyzed by a thermostable α-glucosidase (EC. 3.2.1.20) in order to produce glucose as the only unwanted specie. This glucose can then be converted to gluconic acid and/or its salts using a glucose-oxidase (EC. 1.1.3.4) in combination with a catalase. Gluconic acid (C6H12O7) is a saccharide derivative which has been recognized as a prebiotic compound. It is also known for its purgative action and proved to be effective for lipid peroxidation prevention. A first option can then be chosen, leaving gluconic acid in the product in order to obtain an original prebiotic product enjoying new prebiotic potential properties due to the combination of both types of prebiotic compounds (IMO and gluconic acid). The second option is to eliminate the gluconic acid from the prebiotic mixture. This separation doesn’t present the same difficulties than for glucose as gluconic acid is charged and can therefore be separated on anion-exchange resins (Dowex AcO-). This overall process, fulfilling the principles of green chemistry and being applicable to produce organic prebiotic, is an elegant solution, from an economical, an environmental, a nutri-functional and a techno-functional point of view. Indeed, it can lead to original prebiotic preparations, with yields close to 100%, by avoiding product loss, as the digestible saccharides portion is converted to gluconic acid. Furthermore, the presence of gluconic acid can provide many functional properties to the prebiotic preparations for their incorporation in food products. [less ▲]

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See detailA Boolean network model of the growth plate
Kerkhofs, Johan ULg; Roberts, Scott J; Luyten, Frank P et al

Poster (2010, October 10)

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See detailEmotional facial expressions decoding in siblings of children with autism
Dethier, Marie ULg; Sojic, Barbara ULg; Blairy, Sylvie ULg

Poster (2010, October 08)

The ability to identify other people’s emotions, including their emotional facial expression (EFE), is fundamental to many social processes. Individuals with autism spectrum disorders (ASD) show deficits ... [more ▼]

The ability to identify other people’s emotions, including their emotional facial expression (EFE), is fundamental to many social processes. Individuals with autism spectrum disorders (ASD) show deficits in several empathy-related processes, including EFE decoding (e.g. Ashwin, Chapman, Colle, & Baron-Cohen, 2007). The object of this study was to investigate the capacity to decode accurately EFE in siblings of children with ASD. Indeed, autism is considered to be substantially influenced by genetic factors and relatives of ASD individuals present different type of deficits including the domains of language, theory of mind, and executive functioning (e.g., Fombonne, Bolton, Prior, Jordan, & Rutter, 1997). Fifteen 6-to-15 years old siblings of children with ASD were compared to matched siblings of typically developing children on a decoding task of adults EFE. The children had to match a story depicting an adult in an emotional situation to a picture of an adult EFE. The emotions investigated were joy, anger, sadness, fear, surprise, disgust, and contempt. The differences of EFE accuracy between siblings of ASD and siblings of typically developing children are discussed in reference to the characteristics of the relationship with their ASD brother or sister. [less ▲]

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See detailOptimisation of new biodegradable microcarriers tailored for tissue engineering
Tsoy, A; Sevrin, Chantal ULg; Kottgen, C et al

Poster (2010, October 07)

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See detailDiscussing the validation of high-dimensional probability distribution learning with mixtures of graphical models for inference
Schnitzler, François ULg

Poster (2010, October 06)

Exact inference on probabilistic graphical models quickly becomes intractable when the dimension of the problem increases. A weighted average (or mixture) of different simple graphical models can be used ... [more ▼]

Exact inference on probabilistic graphical models quickly becomes intractable when the dimension of the problem increases. A weighted average (or mixture) of different simple graphical models can be used instead of a more complicated model to learn a distribution, allowing probabilistic inference to be much more efficient. I hope to discuss issues related to the validation of algorithms for learning such mixtures of models and to high-dimensional learning of probabilistic graphical models in general, and to gather valuable feedback and comments on my approach. The main problems are the difficulties to assess the accuracy of the algorithms and to choose a representative set of target distributions. The accuracy of algorithms for learning probabilistic graphical models is often evaluated by comparing the structure of the resulting model to the target (e.g. Number of similar/dissimilar edges, score BDe etc). This approach however falls short when studying methods using a mixture of simple models : individually, these lack the representation power to model the true distribution, and only their combination allows them to compete with more sophisticated models. The Kullback-Leibler divergence is a measure of the difference between two probability densities, and can be used to compare any model learned from a dataset to the data generating distribution. For computational reasons, I however had to resort to a Monte Carlo estimation of this quantity for large problems (starting at around 200 variables). Since probabilistic inference is the ultimate motivation for building these models, and not probability modelling, a more meaningful measure of accuracy could be obtained by comparing mixtures against a combination of state of the art model learning and approximate inference algorithms. However, the exact inference result cannot be easily assessed for interesting target distributions, since the use of mixtures is precisely considered because exact inference is not possible on said targets, and approximate inference would introduce a bias. Selecting a target distribution used to generate the data sets on which the algorithms are evaluated also proved a challenge. The easiest solution was to generate them at random (although different approaches can be designed). These models are however likely to be rather different from real problems, and thus constitute a poor choice to assess the practical interest of mixture of models. Methods (e.g. linking multiple copies of a given network) have been developed to increase the size of models known by the community (e.g. the alarm network), and the obtained graphical models have been made available. These could however still be far from the kind of interactions present in a real setting. A better way to proceed could be to generate samples based on the equations describing a physical problem, to learn a probabilistic model as best as possible from this high-dimensional dataset, and to use it as target distribution. [less ▲]

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See detailCongo River 2010
Darchambeau, François ULg; Borges, Alberto ULg; Wabakanghanzi, José Nlandu et al

Poster (2010, October 04)

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See detailNew strategy to in vitro evaluation of antimalarial drugs demonstre advantage of Artemisia annua crude extract on antimalarial effect by its tools to cross intestinal barrier
Melilo de Magalhaes, Pedro; Schneider, Y. J.; Dupont, Isabelle et al

Poster (2010, October 03)

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See detailAnálisis ecomorfológico de la aleta pectoral en damiselas (Perciformes, Pomacentridae) del Pacífico Oriental
Aguilar-Medrano, Rosalia; Frederich, Bruno ULg; De Luna, Efrain et al

Poster (2010, October)

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See detailInterest of vesicular systems in dermal drug delivery
Gillet, Aline ULg; Lecomte, Frédéric ULg; Hubert, Pascale ULg et al

Poster (2010, October)

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See detailPEPTIDE-LOADED LIPOSOMES AGAINST BREAST CANCER: EFFECTIVE PENETRATION IN CELLS OF LONG CIRCULATING pH-SENSITIVE VESICLES
Ducat, Emilie ULg; Deprez, Julie ULg; Peulen, Olivier ULg et al

Poster (2010, October)

Purpose: Print3G, a peptidic antagonist of oncoprotein involved in breast cancer, could reduce the angiogenic development of breast tumors. The necessity of intravenous administration of Print3G led to ... [more ▼]

Purpose: Print3G, a peptidic antagonist of oncoprotein involved in breast cancer, could reduce the angiogenic development of breast tumors. The necessity of intravenous administration of Print3G led to the development of liposomes as drug carriers, combining the protective properties of PEG with the transfection properties of pH-sensitive lipids. The purpose of this work is to compare pegylated pH-sensitive liposomes with a classical formulation of long-circulating liposomes in terms of cellular uptake. Methods: Classical liposomes (SPC:CHOL:mPEG-750-DSPE (47:47:6 mol/mol)) and pH-sensitive liposomes (DOPE:CHEMS:CHOL: mPEG750-DSPE (43:21:30:6 mol/mol)) were compared in terms of size, charge, stability, pH-sensitivity and toxicity by inhibition of cell proliferation. Finally, confocal microscopy was used to study the cellular uptake of liposomes by three cell lines (Hs578t, WI-26 and MDA-MB-231), using 25-nitrobenzoxydiazol-cholesterol as a fluorescent marker of the vesicular membrane and rhodamine in the inner cavity of liposomes. Results: Sizes of 162.8 ± 4.6 nm and zeta potential of -9.3 ± 1.2 mV were obtained for standard liposomes (n=3) while the obtained values for pH-sensitive liposomes (n=3) were respectively of 184.8 ± 3.2 nm and -19.5 ± 2.6 mV. The two formulations were comparable in terms of shape and stability. Concerning the pH-sensitivity study, a significantly higher leakage of the encapsulated material was observed at pH 5 for pH-sensitive liposomes. Confocal pictures obtained with these vesicles on the three cell lines allowed us to visualize the colocalized red and green color with a higher concentration near the nucleus. Conclusion: Long circulating pH-sensitive liposomes are promising drug delivery systems in terms of cellular uptake. Experiments will be performed with biotinylated Print3G to assess its cellular distribution. Moreover, the accumulation of this formulation in breast tumor will be evaluated by in vivo studies. [less ▲]

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See detailPersistance des troubles psychologiques et comportementaux de la démence
Squelard, Gilles ULg; Adam, Stéphane ULg; Missotten, Pierre ULg et al

Poster (2010, October)

est considérable. Actuellement, la sélection d’un traitement dépend de la nature du trouble et de son intensité. La connaissance de leur persistance s’avère importante pour la sélection et l’organisation ... [more ▼]

est considérable. Actuellement, la sélection d’un traitement dépend de la nature du trouble et de son intensité. La connaissance de leur persistance s’avère importante pour la sélection et l’organisation du traitement. Objectifs: Evaluer la persistance des troubles psychologiques et comportementaux dans une population de sujets déments suivis longitudinalement. Méthodes: Nous avons sélectionné 104 sujets déments issus de l’étude Qualidem (Paquay et al., 2004). Tous les sujets sont institutionnalisés et présentent des perturbations cognitives (score MMSE<20). Les troubles psychologiques et comportementaux ont été évalués à 12 mois d’intervalle avec le Neuropsychiatric Inventory (Cummings et al., 1997). Résultats: Les perturbations les plus persistantes sont les idées délirantes (62%), et l’agitation (67%). Le risque de rechute est moins important pour la dépression (54%), les hallucinations (44%), l’exaltation de l’humeur (43%), l’apathie (48%), l’irritabilité (52%), et le comportement moteur aberrant (52%). L’anxiété (38%), la désinhibition (39%), les troubles du sommeil (39%) et de l’appétit (38%) sont les moins persistants. Conclusion: La nature persistante de l’agitation et des idées délirantes suggère que le traitement de ces perturbations doit être prolongé. La nature ponctuelle de l’anxiété, la désinhibition et des troubles de l’appétit et du sommeil suggère qu’un traitement à court terme est plus approprié pour ces perturbations. Ces résultats sont à interpréter par rapport à la définition des troubles induite par le NPI. [less ▲]

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See detailEffect of kynurenic acid on sensitivity to cortical spreading depression in rats
Chauvel, Virginie ULg; Vamos, Eniko; Pardutz, Arpat et al

Poster (2010, October)

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See detailProjet thérapeutique n°99 "Personnes âgées" et travail en réseau
Squelard, Gilles ULg; Vilain, Anne; Jacob, Bernard

Poster (2010, October)

Introduction : En Belgique, le secteur de la santé mentale est en pleine mutation. Afin d’améliorer l’efficacité des soins, les pouvoirs publics ont décidé de réorganiser l’offre de soins par la ... [more ▼]

Introduction : En Belgique, le secteur de la santé mentale est en pleine mutation. Afin d’améliorer l’efficacité des soins, les pouvoirs publics ont décidé de réorganiser l’offre de soins par la réalisation de « circuits et réseaux de soins ». Cette réforme se construit par phases successives, et c’est dans ce cadre qu’ont été activés les projets thérapeutiques. Ces recherches-actions se concrétisent par le développement de la fonction de concertation autour de patients et de leur famille. Leur finalité est d’améliorer la continuité des soins et de proposer des soins sur mesure dont le patient est l’élément central. Le projet thérapeutique n°99 « personnes âgées » vise le maintien dans leur milieu de vie de personnes de plus de 65 ans vivant en Province de Liège et présentant une problématique psychiatrique chronique et complexe. Objectif Communication de nos principales recommandations concernant la mise en place de la fonction de concertation et à la formalisation du travail en réseau dans le tissu gérontologique liégeois. Méthodologie : Le projet thérapeutique n°99 vise les personnes âgées de 65 et plus, habitant en Province de Liège et présentant une pathologie psychiatrique spécifique : démence, abus d’alcool, abus d’opiacés, abus d’hypnotiques, anxiolytiques ou sédatifs, schizophrénie, ou trouble schizo-affectif. Le projet repose sur une démarche empirique. Il consiste en l’organisation de réunions de concertation pluridisciplinaires autour de patients et de leur famille dont la finalité est la mise en place d’un suivi individualisé. Nous nous appuyons sur ces situations cliniques chroniques et complexes pour mettre en place des procédures de travail en réseau. Résultats : Nous avons suivi environ 80 patients dont l’âge moyen est de 80.1 ans. La plupart des patients suivis (80%) présentent une démence. La coordination du projet opère un travail sur deux axes distincts: l’axe institutionnel et professionnel, et le niveau clinique. L’action ciblant le réseau vise le développement d’une culture de travail en réseau. Celle-ci se construit progressivement à travers une sensibilisation des professionnels au travail de concertation pluridisciplinaire. En ce qui concerne le travail clinique, nous nous sommes focalisés sur la gestion de la concertation pluridisciplinaire. Ce travail se décompose en trois phases : la phase préparatoire, la réunion de concertation, et le suivi. Des procédures de travail ont été mises en place pour en faciliter l’organisation (plan de service, agent de suivi). Conclusion : Le développement de la fonction de concertation a suscité un remaniement dans le mode de fonctionnement des professionnels de terrain, souvent peu familiarisés au travail en réseau et à la collaboration « extérieure ». L’instauration de la culture de travail en réseau via un accompagnement, une sensibilisation, et la mise à disposition de procédures favorisant la collaboration interdisciplinaire s’avère nécessaire. Outre les bénéfices cliniques relevés, il apparaît après trois ans de fonctionnement que la concertation autour de personnes souffrant de problématiques chroniques complexes constitue un moyen efficace de maintien du lien interprofessionnel, et facilite la connaissance et la collaboration des partenaires. [less ▲]

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See detailEssential and non-essential elements in the eggs of sea turtles from the Lesser Antilles
Leroy, Céline; Dyc, Christelle ULg; Bouquegneau, Jean-Marie ULg et al

Poster (2010, October)

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See detailShading and alterations of the sediment: in situ experiments to mimic impacts of fish farms on a Mediterranean coastal ecosystem
Pete, Dorothée ULg; Velimirov, Branko; Lepoint, Gilles ULg et al

Poster (2010, October)

In the Mediterranean coastal zone, the seagrass Posidonia oceanica forms underwater meadows which sustain a high biodiversity and has many important roles (production of oxygen, protection of coasts ... [more ▼]

In the Mediterranean coastal zone, the seagrass Posidonia oceanica forms underwater meadows which sustain a high biodiversity and has many important roles (production of oxygen, protection of coasts, ...). Unfortunately, they are more and more threaten, notably by the increasing development of aquaculture. Indeed, fish farms increase the biomass of phytoplankton organisms in the water column (leading to a shading effect) and modify the sediment, which entail the disappearance of the seagrass. To understand the impact of those troubles on P. oceanica and the ecosystem, in situ experiments were led during three months, in STARESO (STAtion de Recherches Sous- marines et Océanographiques; Calvi, Corsica), at a depth of 10 meters. The shading was mimicked by shading frames, which stopped around 50 % of the incident light. To modify the sedimentary compartment, sediment was taken from under the aquaculture of Calvi and added once a week on marked zones in the meadow. Those sites were compared with a control site, situated near them. Measured parameters are: the concentration of nutrients in pore water, grain size, redox potential discontinuity, biomasses of bacteria, organic matter, meiofauna and microphytobenthos within the sediment, the length, width, biomass and chlorophyll a content of P. oceanica leaves and the biomass of epiphytes. During those experiments, P. oceanica was not very affected although some parameters of the sedimentary compartment were modified. These results show that the sedimentary compartment reacts more rapidly to threats then the seagrass itself. So, it could be an interesting tool in quality assessments. [less ▲]

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See detailTop-down role of fish predators in structuring amphipod community associated to Posidonia oceanica seagrass meadows
Sturaro, Nicolas ULg; Lepoint, Gilles ULg; Panzalis, Pier et al

Poster (2010, October)

Despite the major ecological importance of Posidonia oceanica seagrass meadows in the Mediterranean Sea, little attention has been given to community structuring forces by predators in this ecosystem. To ... [more ▼]

Despite the major ecological importance of Posidonia oceanica seagrass meadows in the Mediterranean Sea, little attention has been given to community structuring forces by predators in this ecosystem. To better understand the mechanisms by which fishes affect amphipod community structure associated to P. oceanica beds, experimental manipulations of predation intensity (enclosure and exclusion cages) were set up in the Tavolara-Punta Coda Cavallo Marine Protected Area (Sardinia, NW Mediterranean). In totally protected area, where high abundance of predatory fishes is recorded, we have performed exclusion experiment. Total amphipod abundance increased at the end of the experiment, but diversity and specific composition were unaffected. At the species taxonomic level, significant increase in abundance between treatments appeared only for Caprella acanthifera. In partially protected area, where fish abundance is lower and amphipod abundance higher, individuals of the carnivorous fish Coris julis (Labridae) were enclosed in cages to provide an estimate of the potential for this species to impact on amphipod community. There was a small but measurable effect on amphipod abundance between enclosures and unmanipulated controls. A few dominant amphipod species responded to fish predation by significant decrease in abundance. These results suggest that fish predation may determine the relative abundances in the community of some amphipod species and to some degree, through an untested link with habitat complexity, determine the spatial distribution of amphipod abundance within P. oceanica beds. Moreover, patterns observed at the species level, suggest parallel complex interactions probably mainly related to ecological behaviour of amphipod species. [less ▲]

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See detailIntroduction of the asymptotic study of the estimation of the error distribution in right censored and selection biased regression models
Laurent, Géraldine ULg; Heuchenne, Cédric ULg

Poster (2010, October)

Consider the regression model Y = m(X) + σ(X) Ɛ where m(X) = E[Y|X] and σ²(X)=Var[Y|X] are unknown smooth functions and the error Ɛ , with unknown distribution, is independent of the covariate X. The pair ... [more ▼]

Consider the regression model Y = m(X) + σ(X) Ɛ where m(X) = E[Y|X] and σ²(X)=Var[Y|X] are unknown smooth functions and the error Ɛ , with unknown distribution, is independent of the covariate X. The pair (X;Y) is subject to generalized bias selection and the response to right censoring. We construct a new estimator for the cumulative distribution function of the error Ɛ , where the estimators of m(.) and σ²(.) are obtained by extending the conditional estimation methods introduced in de Uña-Alvarez and Iglesias-Perez (2008). The asymptotic properties of the functions m(.) and σ(.) are obtained. A bootstrap technique is proposed to select the smoothing parameter involved in the procedure. This method is studied via extended simulations and applied to real unemployment data. [less ▲]

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See detailMir-146a : A new angiostatic miRNA with tumor-suppressive properties
Halkein, Julie ULg; Castermans, Karolien; Malvaux, Ludovic et al

Poster (2010, October)

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See detailTree species effects on soil microbial activities in a young stand
Guillaume, Patricia ULg; Bazgir, Masoud; Carnol, Monique ULg et al

Poster (2010, October)

Tree species effects on soil characteristics and biogeochemistry are mediated by several factors including microclimatic conditions, ground vegetation cover, quality and quantity of litter and roots ... [more ▼]

Tree species effects on soil characteristics and biogeochemistry are mediated by several factors including microclimatic conditions, ground vegetation cover, quality and quantity of litter and roots exudates, interception of atmospheric particules and aerosols, as well as secondary metabolites from litter. Moreover, the effects depend on the activities and characteristics of the microbial populations. Due to complex interactions, tree species effects on biogeochemical cycles may vary according to soil type, site characterisitcs and history, and climate. However, these questions about tree species effects on biogeochemical cycles are of central interest to forested ecosystems functions, such as soil quality (restoration) and soil water protection. This work is part of a long-term study on concentrations and fluxes in main compartments of 2 forested watersheds (Waroneu and Robinette, east Belgium) in relation with forest management. After 2 spruce generations, the Robinette catchment was partially clear-cut in 1996. Since 1998, this watershed is experiencing an ‘extensive’ afforestartion with a mixture of main and secondary tree species, adapted to specific site conditions. This site, provides the opportunity to study tree species effects on the same soil, with the same history. Morever, tree species have different ecological characteristics: N2 fixing species (Alnus glutinosa), secondary broadleaved species (Betula pendula, Sorbus aucuparia, Salix aurita), main broadleaved sepcies (Quercus robur and Fagus sylvatica) and spruce (Picea abies). Here, we analysed the effects of tree species on soil pH and soil microbial activities in the organic layer in relation to carbon and nitrogen cycles: microbial biomass, basal respiration, labile carbon, nitrogen net mineralisation and potential nitrification. Twelve years after plantation, our results showed differences below the different tree species: (1) a higher microbial biomass and a higher substrate use efficiency and organic matter accessbility for microbial populations below spruce as compared with other tree species; (2) higher pH and microbial biomass below secondary than below main broadleaved species; (3) an enhanced nitrification below alder; (4) a higher leaching of nitrate below broadleaved species than below spruce. These results show a short term impact of forest tree species on microbial activities in upper soil layers. Results are discussed in relation to ecological characteristics of tree species. [less ▲]

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See detailRevisited rules of papilla level adjacent to single-tooth dental implants according recent implant designs. A retrospective study.
LECLOUX, Geoffrey ULg; Beaujan, Laurie; ROMPEN, Eric ULg et al

Poster (2010, October)

Background: According to Choquet and Tarnow principles (2001), a papilla is always present if the distance from the base of the contact point between two crowns to the crest of the bone is 5 mm or less ... [more ▼]

Background: According to Choquet and Tarnow principles (2001), a papilla is always present if the distance from the base of the contact point between two crowns to the crest of the bone is 5 mm or less. The arrival of new designs in implantology suggested more tissue preservation and so a possible evolution for the aesthetic treatments. Aim: This study was designed to determine whether the distance from the base of the contact point to the crest of the bone would correlate with the presence or absence of the interproximal papillae adjacent to single-tooth implants using the recent implant designs. Methods: A clinical, photographical and radiographical retrospective evaluation of the papilla level around single dental implants and their adjacent teeth was performed in the anterior maxilla in 34 patients restored with 39 implants. These implants were loaded at least 6 months and present a modified profile (Implants : NobelConcept and NobelActive, Nobel Biocare ; Bone Level, Straumann. Abutments : Platform-switching and curvy). Sixty-three papillae were available for evaluation. The Jemt index, the vertical distance between the base of the contact point and the bone crest, the distance from the papilla level to the contact point were measured. The measurements were rounded off to the nearest 0,001 millimeter and used with calibration computer programs (ImageJ) to be analyzed. The statistical analyses were realized with SAS program (SAS Institute, Cary, NC, USA). Results: When the measurements from the contact point to the crest of bone was 5 mm or less, the papilla was present 100% of the time. When the distance was 6 mm, the papilla was present 96% of the time. And when the distance was 7 and 8 mm, the papilla was present 57,14% and 50% of the time. Conclusions and clinical implications: These results show the influence of the bone crest on the presence or absence of papillae between implants and adjacent teeth. The data also show a positive influence for recent designs of implant and abutment systems. This designs shift the actual critical bone distance under the contact point from 5mm to 6mm and could improve the aesthetic outcome. [less ▲]

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See detailEmotional competencies of the sport coach: A qualitative approach within a physical activity programme for multiple sclerosis patients. A case study
Mouton, Alexandre ULg; Defossa, Clément; Cloes, Marc ULg

Poster (2010, October)

The main purposes of this study were to describe the intervention and to link emotional competencies and behaviors of the coach in a physical activity programme for MS patients. We wanted also to close ... [more ▼]

The main purposes of this study were to describe the intervention and to link emotional competencies and behaviors of the coach in a physical activity programme for MS patients. We wanted also to close this study proposing recommendations to improve MS patient physical activity management. [less ▲]

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See detailVHHs as model proteins to investigate amyloid fibril formation: effect of seeding and cross-seeding on aggregation kinetics and stability of fibrils
Chavignon, Chloé ULg; Dumoulin, Mireille ULg; Pardon, Els et al

Poster (2010, October)

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidosis are known so far, amongst ... [more ▼]

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidosis are known so far, amongst which Alzheimer's disease, type II diabetes and immunoglobulin amyloidosis [1]. Although the mechanism of amyloid fibrils formation at the molecular level is not yet completely understood, it has been shown that the capacity to form amyloid fibrils in vitro is an intrinsic property of all polypeptide chains [1]. The choice of model proteins to investigate the aggregation process in vitro is therefore no more restrained to proteins involved in amyloidosis but can be settled on a wide variety of proteins. In this study, we have chosen to investigate the mechanism of amyloid fibrils formation by two variable domains of camelid heavy-chain antibodies (referred to as VHHs or nanobodies), cAb-HuL6 and cAb-BcII10, and this choice was motivated by the following reasons: - First, VHHs are small monomeric proteins (~14 kDa) presenting a high stability and a high solubility [2], which permits their expression with a high yield (5-20 mg.L-1). - Second, a wide range of stable mutants of these two VHHs is available. Mutations located at the disulfide bond [3,4] and the CDRs [3] have been introduced. Characterisation of the aggregating properties of these mutants will allow the investigation of the impact of these structural elements on the process of fibril formation. In order to determine conditions in which cAb-HuL6 and cAb-BcII10 are more susceptible to form intermediates and thus amyloid fibrils, heat-induced unfolding experiments at pHs comprised in a range from 2,5 to 9,5 have been monitored by intrinsic fluorescence, ANS binding and far-UV circular dichroism. Then, aggregation experiments have been performed in the selected conditions and the presence of amyloid fibrils has been observed by thioflavin T fluorescence experiments and electron microscopy. The kinetics of aggregation obtained in the absence and the presence of seeding/cross-seeding allowed to identify the regions of the protein which could be involved in the formation of fibrils. [1] Chiti and Dobson, Annu. Rev. Biochem., 75, 2006, 333-366. [2] Dumoulin et al., Protein Sci., 11, 2002, 500-515. [3] Saerens et al., J. Mol. Biol., 352, 2005, 597-607. [4] Saerens et al., J. Mol. Biol., 377, 2008, 478-488. [less ▲]

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See detailPedagogical supervision and initial teachers’ education- an international research project
Albuquerque, Alberto; Pinheiro, Claudia; Aranha, Agata et al

Poster (2010, October)

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See detailSport as a support for school reintegration. A case study
Cloes, Marc ULg; Maraite, Anne-Aymone ULg; Dijon, François

Poster (2010, October)

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See detailMotives to begin physical education studies. A case study
Kerfes, Nabil; Alem, Jaouad; Cloes, Marc ULg

Poster (2010, October)

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See detailEvolution nutritionnelle des patients hémodialysés du CHBAH
Meurice, C.; Smelten, Nicole; Furnemont, L. et al

Poster (2010, September 30)

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See detailCold adaptation of proteins: a biophysical study of a psychrophilic alpha-amylase and its stabilized mutants
Cipolla, Alexandre ULg; D'Amico, Salvino ULg; Feller, Georges ULg

Poster (2010, September 28)

Habitats of permanently cold temperature, like polar regions for example, have been colonized by a great variety of psychrophilic organisms producing enzymes adapted to function efficiently in these cold ... [more ▼]

Habitats of permanently cold temperature, like polar regions for example, have been colonized by a great variety of psychrophilic organisms producing enzymes adapted to function efficiently in these cold environments. According to the hypothesis developed in our laboratory, the adaptation to cold temperature involves relationships between activity, flexibility and stability. Even if activity and stability are not physically linked in proteins 1, the consensus for the adaptive strategy is to take advantage of the lack of selective pressure for stable proteins to lose stability, therefore increasing the flexibility or mobility of the enzyme at low temperatures that restrict molecular motions. 2 Working on alpha-amylase, we have investigated the role of weak interactions in thermal adaptation of proteins by site-directed mutagenesis. We have built two multiple-mutants (Mut5 and Mut5CC) of the psychrophilc alpha-amylase (AHA) from the Antarctic bacterium, Pseudoalteromonas haloplanktis. The single mutations were selected by comparison of the presence of weak interactions in a mesophilic chloride-dependant homolog from pig pancreas, PPA. The study of selected single mutations prompt us to construct two multiple-mutants, Mut5 and Mut5CC, carrying 5 and 6 additional weak interactions found in PPA, that showed an increased stability and a lower activity at 25 °C.3 We have compared AHA, Mut5 and Mut5CC with additional methods like differential scanning calorimetry, thermal and chemical unfolding in order to determine the gain in stability. We also studied the flexibility or breathing of the enzymes by acrylamide-induced fluorescence quenching and we determined the optimum activity temperature for the three amylases. In order to investigate the kinetic origin of the gain in stability 4 for the two multiple-mutants, we studied in a first step the kinetic unfolding and refolding by GdmCl of the three amylases by manual methods following fluorescence signal at 15°C. The newly introduced weak interactions stabilized the proteins, protected them against heat and chemical unfolding and also induced an effective loss of flexibility. In addition, the two multiple-mutants exhibit a different optimum activity temperature than AHA. The first result in manual kinetic studies seems to show a similar refolding phase but a difference between the three amylases in the unfolding phase. This is in correlation with results of Dieter, P et al 4. These results and those of the previous work 3, unambiguously support the capital role of weak interactions in the balance between activity, flexibility and stability and provide a better knowledge of the adaptation of enzymes to cold temperatures. [less ▲]

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See detailKnowledge-based approaches for degradation mapping in arid rangeland
Mahyou, Hamid ULg; Tychon, Bernard ULg; balaghi, Riad

Poster (2010, September 28)

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See detailComparaison of the flower scents of two Impatiens species
Marlet, Christelle ULg; Lognay, Georges ULg; Jacquemart, Anne-Laure et al

Poster (2010, September 27)

The Giant balsam, Impatiens glandulifera Royle (Balsaminaceae) was introduced in Europe from the Himalayas as a garden ornamental plant in 1839. Due to its great success from nursery gardeners (1), it is ... [more ▼]

The Giant balsam, Impatiens glandulifera Royle (Balsaminaceae) was introduced in Europe from the Himalayas as a garden ornamental plant in 1839. Due to its great success from nursery gardeners (1), it is now considered as one of the 100 worst invasive species in Europe. On the contrary, the native I. noli-tangere L. is considered to be in decline. Both species are annuals that absolutely need reproductive output to maintain or extend their populations. High fecundity has frequently been associated with invasiveness. However, traits controlling the reproductive success like pollinator attractiveness have not yet been assessed. The alien species is profusely visited by bumblebees which constituted the main visitors and efficient pollinators (2-3). The native also presents traits linked to insect attractiveness. Flowers produced similar quantities of nectar with the same sugar concentration and composition than the exotic. Therefore, the native could be considered as valuable source of nectar for pollinators. Despite the fact that nectar reward is likely to influence pollinator attractiveness, I. noli-tangere only occasionally received visits. Indeed, a flower of the native is 40 times less visited than a I. glandulifera flower (3). Differences in visitation rates may be explained by several factors, as floral scents, UV patterns or floral display. In summer 2009, a first comparative study of floral scents between these two species was performed. An active sampling in a small glass chamber able to contain single flower left on the plant had been performed on a sorbent tube with Carbograph and Tenax TA. The volatiles of floral scents had been analysed by thermal desorption coupled to gas chromatography and mass spectrometry (4, 5). The composition of flower scents on the basis of area percent of identified peaks had been compared. Terpenes (d-limonene, α-terpinolene, p-cymene, α and β-terpineol) were emitted by the flowers of both species. Higher monoterpenes proportion in area percent was detected for the native species. Terpenes such as β-myrcene, 1,4-cineole, eucalyptol, carveol were absent in the flower scent of the alien I. glandulifera. However, the alien presented a higher alcane proportion. The implication of terpenes in the attractiveness of bumblebees will be studied. [less ▲]

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See detailOù ai-je posé mes clefs ? Les erreurs de glissement de mémoire dans le vieillissement normal et pathologique
Guerdoux, Estelle; Dressaire, Deborah; Martin, Sophie et al

Poster (2010, September 24)

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See detailDéficits d’inhibition dans le vieillissement normal et la maladie d’Alzheimer: pas d’atteinte spécifique aux niveaux de traitement perceptif ou moteur
Stawarczyk, David ULg; Grandjean, Julien ULg; Salmon, Eric ULg et al

Poster (2010, September 24)

Introduction. Une diminution des capacités d’inhibition est fréquemment observée dans le vieillissement normal et la maladie d’Alzheimer. Toutefois, peu d’études ont exploré la généralité de ces déficits ... [more ▼]

Introduction. Une diminution des capacités d’inhibition est fréquemment observée dans le vieillissement normal et la maladie d’Alzheimer. Toutefois, peu d’études ont exploré la généralité de ces déficits au sein d’un groupe unique de participants. Objectif. Déterminer si les déficits d’inhibition présents dans le vieillissement normal et la maladie d’Alzheimer sont en accord avec la distinction entre inhibition perceptive et inhibition motrice proposée par Dempster et Corkill (1999). Méthode. Nous avons administré une large batterie d’épreuves d’inhibition à un groupe de participants jeunes, de participants âgés sains et de patients souffrant de maladie d’Alzheimer. L’inhibition perceptive a été évaluée au moyen des épreuves de Stroop et de priming négatif, de la tâche des ailiers et d’une tâche de résolution de conflit perceptif ; l’inhibition motrice a quant à elle été évaluée au moyen de tâches de go/no-go, de stop-signal, d’antisaccade et de résolution de conflit moteur. Résultats. Les résultats obtenus indiquent la présence d’un pattern mixte de déficits, incluant à la fois des épreuves d’inhibition motrice et d’inhibition perceptive, aussi bien chez les sujets âgés sains que chez les patients souffrant de la maladie d’Alzheimer. Des différences qualitatives de performance ont également été observées entre les deux groupes de sujets âgés. Discussion. Nos résultats ne sont pas en la faveur d’une distinction entre inhibition motrice et perceptive. Les déficits d’inhibition observés dans le vieillissement normal peuvent être interprétés dans le sens d’une diminution générale des ressources de traitement tandis que les déficits des patients Alzheimer peuvent être attribués à une capacité réduite de résistance à l’interférence provenant d’informations non pertinentes dans l’environnement externe de la personne. La présence de différences qualitatives de performance entre les deux groupes de sujets âgés semble indiquer que la maladie d’Alzheimer ne consiste pas en une simple accentuation des difficultés déjà observées lors du vieillissement normal. [less ▲]

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See detailComparison and combination of spectroscopic techniques for the detection of counterfeit medicines
Sacré, Pierre-Yves ULg; Deconinck, Eric; De Beer, Thomas et al

Poster (2010, September 23)

During this study, Fourier transform infrared spectroscopy (FT-IR), near infrared spectroscopy (NIR) and Raman spectroscopy were applied to 55 samples of counterfeit and imitations of Viagra® and 39 ... [more ▼]

During this study, Fourier transform infrared spectroscopy (FT-IR), near infrared spectroscopy (NIR) and Raman spectroscopy were applied to 55 samples of counterfeit and imitations of Viagra® and 39 samples of counterfeit and imitations of Cialis®. The aim of the study was to investigate which of these techniques and associations of them were the best for discriminating genuine from counterfeit and imitation samples. Only the regions between 1800-400 cm-1 and 7000-4000 cm-1 were used for FT-IR and NIR spectroscopy respectively. Partial Least Square analysis has been used to allow the detection of counterfeit and imitation tablets. It is shown that for the Viagra® samples, the best results were provided by a combination of FT-IR and NIR spectroscopy. On the other hand, the best results for the Cialis® samples were provided by the combination of NIR and Raman spectroscopy (1400-1190 cm-1). These techniques permitted a clear discrimination between genuine and counterfeit or imitation samples but also the distinction of clusters among illegal samples. This might be interesting for forensic investigations by authorities. [less ▲]

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See detailHydrologic modelling and dendrochronology as tool of site-species adequation assessment in a changing climate context
Sohier, Catherine ULg; Debruxelles, Jérôme ULg; Brusten, Thomas ULg et al

Poster (2010, September 21)

A hydrologic model is related to dendrochronological measurements performed in a 52 years old Spruce stand. The site is situated on a hillside with shallow and acid brown soil in the ecoregion of Ardenne ... [more ▼]

A hydrologic model is related to dendrochronological measurements performed in a 52 years old Spruce stand. The site is situated on a hillside with shallow and acid brown soil in the ecoregion of Ardenne (Wallonia, Southern Belgium). Hydrologic modelling The hydrologic simulation runs from 1971 to 2005 at daily time step. The model is based on an EPIC code, adapted to the site concerning soil reservoirs depth, characteristic water contents, root profile and water uptake. Weather data come from the Royal Meteorological Institute. Outputs from the model are real evapotranspiration, surface runoff; interflows, deep percolation and soil moisture at daily time step. Dendrochronological study Tree ring thickness is measured on 24 core samples extracted from 12 dominant trees of the stand. Annual increments are standardised by the ARIMA function in order to produce the annual deviation of ring thickness. Means of annual deviation for the 12 trees are then related to annual soil drought intensities. Results Years 1976 and 1996 are emphasised by both the modelling outputs and the dendrochronological measurements as very dry. Model shows a severe drought and tree ring shows a very low growth. Years like 1990, 1992, 1994 and 2004 shows a less severe drought event but a drought that occurs in June-July, which seems to penalize spruce’s growth. On the other hand, years 1981 and 1998 show an important growth and a high value of mean soil moisture during June and July. The poster will show how the time evolution of the ARIMA index is related to some meaningful hydrologic indexes. These considerations will allow us to progress towards forecasting forest trees reaction to climate events and change. With this in mind, we will use a climate scenario build up in the frame of the AMICE interreg project. [less ▲]

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See detailPretreatment And Enzymatic Hydrolysis Of Miscanthus x giganteus: Influence Of Process Parameters
Vanderghem, Caroline ULg; Brostaux, Yves ULg; Jacquet, Nicolas ULg et al

Poster (2010, September 21)

Miscanthus x giganteus is a perennial grass which grows rapidly and gives high yields of biomass per hectare. It can be grown in poor quality soil and is non invasive. Due to its high cellulose and ... [more ▼]

Miscanthus x giganteus is a perennial grass which grows rapidly and gives high yields of biomass per hectare. It can be grown in poor quality soil and is non invasive. Due to its high cellulose and hemicellulose content, it has attracted considerable attention as a possible energy crop to produce bioethanol. Pretreatment of lignocellulosic biomass is a key step to unlocking the protective structures so that the enzymatic hydrolysis of the carbohydrate fraction to monosugars can be achieved more easily and with greater yield. In this study, Miscanthus x giganteus was delignified by a chemical pre-treatment process using a mixture of formic acid/acetic acid (1). The treated material was then hydrolyzed. By means of Box-Behnken experimental design and response surface methodology we investigated the effect of cooking time (60, 120 and 180 min), formic acid/acetic acid/water concentration (20/60/20, 30/50/20 and 40/40/20) and temperature (80, 90 and 107°C) on the residual Klason lignin content and the % of digestibility. The optimal pretreatment process parameters were identified. [less ▲]

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See detailProjet thérapeutique n°99 "Personnes âgées"
Squelard, Gilles ULg; Vilain, Anne; Jacob, Bernard

Poster (2010, September 21)

Introduction Dès avril 2011, la fonction de concertation fera l’objet d’un financement structurel pour les soins en santé mentale en Belgique. Cette fonction va se construire en partie sur base de ... [more ▼]

Introduction Dès avril 2011, la fonction de concertation fera l’objet d’un financement structurel pour les soins en santé mentale en Belgique. Cette fonction va se construire en partie sur base de l’expérience des projets thérapeutiques « réseaux et circuits de soins ». Ces recherches actions se concrétisent par l’organisation de réunions de concertation pluridisciplinaires autour de situations chroniques et complexes. Plus spécifiquement, le projet thérapeutique n°99 « personnes âgées » a pour objectifs de favoriser le maintien à domicile de personnes âgées, de garantir la continuité des soins et du projet de vie, d’améliorer la qualité de la réintégration au domicile, d’éviter les réhospitalisations, de mieux cerner les conditions d’une réhospitalisation, et d’aborder les problématiques d’isolement, de solitude, voire de maltraitance. Objectif Proposition d’un modèle organisationnel de travail en réseau sur base de l’expérience du projet thérapeutique n°99 « Personnes âgées » en Province de Liège. Méthodologie Le projet thérapeutique n°99 vise les personnes âgées de 65 ans et plus, habitant en Province de Liège et présentant une pathologie psychiatrique spécifique : démence, abus d’alcool, abus d’opiacés, abus d’hypnotiques, anxiolytiques ou sédatifs, schizophrénie, ou trouble schizo-affectif. Le projet repose sur une démarche empirique. Il consiste en l’organisation de réunions de concertation pluridisciplinaires autour de patients et de leur famille dont la finalité est la mise en place d’un suivi individualisé. Nous nous appuyons sur ces situations cliniques pour mettre en place des procédures de travail en réseau. Résultats L’âge moyen de la population suivie est de 79,21 ± 6.71. Elle se constitue majoritairement d’hommes (51%). Les diagnostiques les plus fréquents à l’inclusion sont la démence (Alzheimer, vasculaire,..) et l’abus d’alcool. La mise en place et la pérennisation de la fonction de concertation a nécessité et nécessite encore une sensibilisation des professionnels (équipes et pouvoir organisateur), quant à l’intérêt de la concertation et à ses modalités organisationnelles. Avec les partenaires du projet, nous avons développé des procédures de travail en réseau destinées à faciliter et à structurer les échanges. Pour chaque situation, autour de l’usager, nous identifions un agent de suivi (référent du patient) qui assure des fonctions de liaison, d’évaluation et de bienveillance. Nous nous appuyons sur un outil « le plan de soins individualisé - PSI » qui motive la définition d’objectifs clairs, identifie le rôle de chaque partenaire, et l’échéance à respecter pour la prise en charge. Conclusion L’expérience du projet thérapeutique n°99 s’est avérée extrêmement fructueuse, par l’atteinte des objectifs centrés sur l’usager et leur famille, mais également à travers les nombreux échanges interprofessionnels. La concertation pluridisciplinaire constitue un excellent moyen de connaissance des partenaires, amenant une représentation plus concrète de leur mode de fonctionnement et de leur offre de soins. Le développement de la fonction de concertation a suscité un remaniement organisationnel important chez les professionnels, souvent peu familiarisés à la collaboration « extérieure ». Sa mise en place dans le champs de la santé mentale requiert le développement de procédures de travail en réseau ainsi qu’un accompagnement des professionnels tant au niveau institutionnel qu’autour de l’usager et de leur famille. Une responsabilisation des partenaires devra appuyer cette démarche pour garantir leur investissement dans un processus de travail en réseau. L’efficacité de celui-ci repose sur l’implication de l’ensemble des partenaires disponibles sur une zone géographique donnée. [less ▲]

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See detailInfluence d'un avis pharmaceutique à l'admission sur les prescriptions potentiellement inappropriées chez des patietns hospitalisés en gériatrie
Samalea Suarez, Audrey ULg; Petermans, Jean ULg; Van Hees, Thierry ULg

Poster (2010, September 18)

1) Introduction L’iatrogénie médicamenteuse, très courante chez la personne âgée, est un problème de santé publique. Cependant, il est possible d’en limiter les effets par une prescription adéquate ... [more ▼]

1) Introduction L’iatrogénie médicamenteuse, très courante chez la personne âgée, est un problème de santé publique. Cependant, il est possible d’en limiter les effets par une prescription adéquate. Divers outils spécifiques, tels que les listes de Beers (1) ou de Laroche (2) permettent d’évaluer le caractère inapproprié des médications. 2) Objectifs Evaluer l’influence d’un avis pharmaceutique, rédigé conjointement à une anamnèse médicamenteuse, sur les prescriptions potentiellement inappropriées chez les patients hospitalisés en gériatrie au CHU de Liège. 3) Méthodologie Etude prospective avec contrôle historique. Analyse des médicaments potentiellement inappropriés à l’admission et à la sortie pour les patients hospitalisés entre octobre et décembre (2008 = contrôle historique ; 2009 = intervention) dans le service de gériatrie du CHU de Liège, provenant du domicile et consommant minimum 3 médicaments à l’arrivée à l’hôpital. L’intervention consistait à réaliser une anamnèse médicamenteuse assortie d’un avis pharmaceutique relevant les prescriptions potentiellement inappropriées. Les prescriptions étaient considérées comme inappropriées si elles étaient reprises sur une liste préalablement établie, basée sur les critères de Beers et de Laroche. Les données concernant les traitements à l’admission proviennent du dossier médical informatisé pour le groupe historique et de l’anamnèse réalisée par le pharmacien pour le groupe intervention. Les médications de sortie, pour les 2 groupes, sont issues du dossier médical informatisé. 4) Résultats 50 patients ont été recrutés dans chaque groupe. L’âge moyen (82,9±6,0) et la répartition homme/femmes (34/66) sont semblables. Le nombre moyen de médicaments à l’admission et à la sortie ne diffère pas entre les deux groupes (A : 7,7±2.9 vs 8,4±3.2, p=0,26 ; S : 8,2±3,0 vs 7,7±2,5, p=0,33). On observe cependant une réduction du nombre de médicaments entre l’admission et la sortie dans le groupe intervention (0,52±2.6 vs -0,72±2,9, p=0,028). Le nombre moyen de médicaments potentiellement inappropriés à l’admission est semblable entre les deux groupes mais diminue significativement à la sortie dans le groupe intervention (A : 1,2±1,02 vs 1,1±1,02, p=0,78 ; S : 0,94±0,82 vs 0,56±0,54, p=0,019). Au sein de chaque groupe, le nombre de médicaments inadaptés diminue entre l’admission et la sortie mais la différence est plus marquée pour le groupe intervention (2008 : p=0,044 ; 2009 : p<0,0001). On observe également une diminution de la durée d’hospitalisation entre 2008 et 2009 (médiane 17,5 vs 13 jours p=0,008). 5) Conclusion Malgré un échantillon limité de patients et le caractère rétrospectif de certaines données, ces résultats démontrent la valeur ajoutée du pharmacien clinicien et d’un avis pharmaceutique à l’admission sur la qualité de la prescription médicamenteuse à la sortie d’une hospitalisation gériatrique. Cette expertise importante contribue à la prise en charge pluri-disciplinaire du patient âgé fragilisé. [less ▲]

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See detailBEHAVIOURAL ECOLOGY OF LONG-TAILED MACAQUES IN THE CONTEXT OF URBAN COMMENSALISM: A COMPARISON STUDY BETWEEN BANGKOK (THAILAND) AND BALI (INDONESIA)
Brotcorne, Fany ULg; Huynen, Marie-Claude ULg; Beudels-Jamar, Roseline et al

Poster (2010, September 16)

The quest for coexistence with non-human primates requires an extensive analysis of the growing commen-salism phenomenon. The long-tailed macaque (Macaca fascicularis), a very successful commensal species ... [more ▼]

The quest for coexistence with non-human primates requires an extensive analysis of the growing commen-salism phenomenon. The long-tailed macaque (Macaca fascicularis), a very successful commensal species, adapts remarkably to anthropogenic habitats. Studies in several Asian locations raised the issue of hu-man-macaque conflict related to human health and safety as well as to conservation threats on macaques. Here, we aimed at assessing the anthropogenic impact (human presence and provisioning frequency) on the eco-behavioural profile of two populations living commensally with humans in Bangkok (Thailand) and Ubud (Padangtegal, Bali). We used an identical focal and scan sampling methodology during two three-month study periods in 2007 (Bangkok) and 2009 (Bali). Despite different ecological conditions, the two populations showed a strongly similar activity budget. Resting was the most common activity (40% vs. 35%), followed by feeding (28% vs. 24%), moving (15% vs. 14%) and affiliations (14% vs. 19%). Agonistic behaviours were slightly more frequent in Bali (0.6% vs. 3.0%), maybe due to higher population density (8.5/ha vs. 14.2/ha). Contrary to previous studies, we did not find any impact of provisioning on agonistic interactions. Concerning diet composition, proportions of natural vs. provisioned food and proportions of various food categories were consistent between the two sites. Since the ecological conditions were different, the anthropogenic factors are likely to explain the strong consistency in eco-behavioural profile of the two populations. Further studies are planned on three other populations to assess the specific impact of commensalism on behavioural ecology and derive the implications for long term population trends. [less ▲]

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See detailMicrobiological Analyses of Raw Milk Cheeses from Walloon Farms
Sanchez-Alcaraz, Maria-Thérésa ULg; Godrie, Thérèse ULg; Helleputte, Murielle ULg et al

Poster (2010, September 16)

For several years now, the Walloon Region has been supporting a project to help and follow farmers through their diversification activities: la Cellule Qualité Produits Fermiers (CQPF). Among their ... [more ▼]

For several years now, the Walloon Region has been supporting a project to help and follow farmers through their diversification activities: la Cellule Qualité Produits Fermiers (CQPF). Among their partners, the Process and Quality Engineering Laboratory in the Department of Food Technology of the Agricultural University of Gembloux is in charge of the hygienic supervision, ensuring the implementation of self-evaluation systems based on the HACCP principles and good hygiene practice guidelines. For four years, the CQPF has been following almost a hundred Walloon cheesemaking artisans using raw milk from cow, goat and ewe. According to the Commission Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs, analyses of microbiological pathogens have to be performed regularly to guarantee healthy and hazard free raw milk products. The results of microbiological analyses from raw milk cheeses of our artisans between January 2007 and June 2010 are presented below. We were interested on showing firstly, if the quality of the Walloon cheeses follow the standards of food safety criteria established by the European regulation, and secondly, if there is a trend for pathogen contamination throughout the year. [less ▲]

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See detailBMP signalling in growth plate chondrocytes: a Boolean modelling approach
Kerkhofs, Johan ULg; Roberts, Scott J; Van Oosterwyck, Hans et al

Poster (2010, September 15)

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See detailInfluence of Brain-Derived Neurotrophic Factor val66met human polymorphism on declarative memory consolidation during sleep
Mascetti, Laura ULg; Foret, Ariane ULg; Matarazzo, Luca et al

Poster (2010, September 15)

Objectives The Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin which in the adult brain, regulates long-term potentiation and has been involved in the build up of the homeostatic sleep pressure ... [more ▼]

Objectives The Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin which in the adult brain, regulates long-term potentiation and has been involved in the build up of the homeostatic sleep pressure in rodents. In humans, valine (val) to methionine (met) substitution in the 5’ pro-region of the BDNF protein is associated with poorer episodic memory. Neurons transfected with met-BDNF-Green Fluorescence Protein showed lower depolarization-induced secretion, while constitutive secretion is unchanged. Here, we hypothesized that the differences in BDNF release determined by this polymorphism would influence sleep-dependent memory consolidation and that in comparison with the met-carriers (val/met or met/met), val/val individuals would show higher memory performance after one night of sleep rather than an immediate retrieval session. Methods Participants encoded a series of neutral faces in the afternoon. Retrieval sessions took place one hour after the encoding session, and in the following morning, after a night of polysomnographic-monitored sleep. During retrieval, studied faces and new ones were presented in random order. For each stimulus, the subjects indicated whether they could retrieve the encoding episode with (“Remember” response), or without details (“know” response), or if they thought the item had not been presented during encoding (“New” response). Results A repeated-measure ANOVA on discrimination index (d’) showed significant effects of group (F(1, 22)=4.66, p=0.042) and session (F(1, 22)=12.21, df=1, p=0.002). Although the group by session interaction was not significant (F(1, 22)=1.84, p=0.188), exploratory planned comparisons showed that at immediate retrieval, d’ was not significantly different between groups (val/val, d’ = 1.94±0.16; met-carriers, d’= 1.61±0.14; p>0.5). In contrast, during the second retest (the next day) d’ in the val/val group (d’=2.56±0.23) was significantly higher than in the met-carriers group (d’=1.88±0.21; p=0.041). Likewise, a between-session enhancement in d’ was detected only in the val/val population (p=0.003). Conclusion Val/val individuals demonstrate higher memory performance than met-carriers after a night of sleep but not at immediate retest. These data suggest that activity-dependent BDNF release promotes memory consolidation during the first post-training hours. Further analysis of the present data set will assess the respective effect of sleep and time on the BDNF-associated delayed memory enhancement. This study was supported by FNRS-FRIA, the University of Liège, and the QEMF. [less ▲]

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See detailTailor-made copolymers for responsive drug delivery nanosystems
Cajot, Sébastien ULg; Jérôme, Christine ULg

Poster (2010, September 15)

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See detailNon Stationary Multiresolution Analysis
Simons, Laurent ULg

Poster (2010, September 13)

An orthonormal basis of wavelets of $L^2(\R)$ is an orthonormal basis of $L^2(\R)$ of type \[ \psi_{j,k}=2^{j/2}\psi(2^j\cdot-k),\quad j,k\in\Z. \] A classical method to obtain such bases consists in ... [more ▼]

An orthonormal basis of wavelets of $L^2(\R)$ is an orthonormal basis of $L^2(\R)$ of type \[ \psi_{j,k}=2^{j/2}\psi(2^j\cdot-k),\quad j,k\in\Z. \] A classical method to obtain such bases consists in constructing a multiresolution analysis. When the mother wavelet $\psi$ depends on the scale (i.e. the index $j$), a non stationary version of multiresolution analysis is then used. It is for example the case in the general context of Sobolev spaces. We generalize different characterizations in the standard theory of wavelets to the case of multi-scales wavelets and non stationary multiresolution analyses. [less ▲]

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See detailAffine bundles are affine spaces
Leuther, Thomas ULg

Poster (2010, September 13)

We show that the category of affine bundles over a smooth manifold M is equivalent to that of affine spaces modeled on locally free modules over the algebra of smooth functions on M.

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See detailSome results about prevalence and strongly monoHölder functions
Kreit, Damien ULg

Poster (2010, September 13)

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See detailRecognisable languages over an algebra
Cavus, Rukiye ULg

Poster (2010, September 13)

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See detailImpaired verbal short-term memory for serial order information in dyslexic adults
Martinez Perez, Trecy ULg; Majerus, Steve ULg; Poncelet, Martine ULg

Poster (2010, September 10)

This study used the distinction of item and order information in verbal short-term memory (STM) to further our understanding of verbal STM impairments in dyslexia. Item STM has been shown to depend on the ... [more ▼]

This study used the distinction of item and order information in verbal short-term memory (STM) to further our understanding of verbal STM impairments in dyslexia. Item STM has been shown to depend on the quality of underlying phonological representations and hence should be impaired in dyslexic participants given their poor phonological processing abilities. On the other hand, order STM represents a specific STM capacity predicting learning capacity for new phonological and orthographic sequences (Majerus et al., 2006; Nithart et al, 2010). If STM impairments contribute to dyslexia, then especially order STM should be impaired, in addition to item STM. [less ▲]

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See detailCategorization and abstraction of construction schemas in children with specific language impairment
Leroy, Sandrine ULg; Duquet, Adèle ULg; Parisse, Christophe et al

Poster (2010, September 10)

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See detailStylistic Issues in New Nigerian Literature
Tunca, Daria ULg

Poster (2010, September 09)

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See detailLinking abstract form and grammatical function : a construction-grammar experiment
Parisse, Christophe; Maillart, Christelle ULg

Poster (2010, September 09)

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See detailSpoken sentence comprehension in children with developmental dyslexia
Leclercq, Anne-Lise ULg; Majerus, Steve ULg; Guasti, Maria Teresa et al

Poster (2010, September 09)

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