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See detailMicroRNA-146a is a causative factor and a specific biomarker for peripartum cardiomyopathy
Halkein, Julie ULiege; Tabruyn, Sébastien ULiege; Haghikia, Arash et al

Poster (2012, April)

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See detailStudy of the microbial diversity in vacuum-packed chilled beef from different origins through a metagenomics approach
Didimo Imazaki, Pedro Henrique ULiege; Taminiau, Bernard ULiege; Nezer, Carine et al

Poster (2012, April)

Despite a diverse initial microbial population, bacterial spoilage of vacuum-packed chilled beef is mainly due to the growth of psychrotrophic bacteria. The study of the microflora of vacuum-packed ... [more ▼]

Despite a diverse initial microbial population, bacterial spoilage of vacuum-packed chilled beef is mainly due to the growth of psychrotrophic bacteria. The study of the microflora of vacuum-packed chilled beef remains a challenge since some members of the microflora may be missed or not identified by cultivation-based methods. The aim of this study was to evaluate the microbial diversity in eight batches of vacuum-packed chilled beef from different origins (Australia, Belgium, Brazil, Ireland and United Kingdom) by metagenomics. Longissimus dorsi muscle samples were homogenized and analysed in early and late stages of their shelf life by metagenomics. The metagenomic assays consisted in DNA extraction, 16S ribosomal RNA gene amplification, pyrosequencing and data analysis. All samples, except for two batches from Australia, presented a high microbial diversity in the beginning of their shelf life. Enterobacteriaceae, Pseudomonas, Burkholderia, Lactobacillus and Sterotrophomonas were some of the major bacteria identified at this stage of storage. The dominant flora (> 80 % of relative abundance) in two Australian batches was composed by Carnobacterium. At the end of the shelf life of the samples, a decrease in microbial diversity was observed in almost all batches. At this stage of storage, Carnobacterium, Lactobacillus, Lactococcus and Enterococcus were some of the major genera identified. Carnobacterium remained the dominant flora in the two Australian batches cited above, which could explain the long shelf life applicable to this meat (140 days) as some Carnobacterium strains induce a biopreservative effect especially by producing bacteriocins with a wide inhibition spectrum. Metagenomics showed to be a very useful tool to study the microbial population of a complex matrix such as meat since some of the identified genera such as Lactobacillus and Carnobacterium are known not to grow or to grow slowly in media commonly used for the isolation and cultivation of total viable counts. [less ▲]

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See detailhe influence of transformational leadership on commitment: New underlying processes
Marique, Géraldine; Stinglhamber, Florence; Hanin, Dorothée et al

Poster (2012, April)

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See detailOrganizational identification and commitment: The impact of POS and prestige
Marique, Géraldine; Stinglhamber, Florence; Desmette, Donatienne et al

Poster (2012, April)

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See detailMapping error due to image geometric correction.
Hallot, Eric ULiege; Cornet, Yves ULiege; Hallot, Pierre ULiege

Poster (2012, April)

Nowadays, image processing and GIS tools are more accessible and available to all. Most of diachronical or historical studies use the image geometric correction with several documents like old maps, plans ... [more ▼]

Nowadays, image processing and GIS tools are more accessible and available to all. Most of diachronical or historical studies use the image geometric correction with several documents like old maps, plans or aerial photographs. They are usually based on low-order polynomial equation to perfom these kinds of simplistic uses. Most of commercial softwares provide us a total or by ground control points RMSE but they don’t offer a overview of the spatial error distribution. More this does not guarantee that its significance for the other points of the image. Do not consider these errors can lead to erroneous observations and misinterpretations like historical moving streams or bank erosion rate. Using a least square adjustment (LSA), allow to recalculate the transformation equation from the pairs of control points coordinates. Assuming that residuals are spatially auto-correlated, we can build error vectors whose direction and magnitude symbolize the error at each point of the image. By another way, LSA manage the precision during transformation process by using a stochastic model in addition of the mathematical model during the transformation. This gives us variance on parameters of the transformation. Knowing the precision of all elements and variance/covariance matrix on transformation parameters, we apply variance propagation on the model. As an output, we obtain confidence ellipses on each transformed points, thus a spatial distribution of error for the entire image. Moreover, this leads us to determine statistically if observed movement are significant or not. The represent error is a complex problem that cannot be solved with a quick fix. In this work, we present an interesting example of using well-known topographic methods to solve image processing problems. [less ▲]

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See detailEvaluation of three immunoassays for serodiagnosis of human Mycoplasma pneumoniae infection
HUYNEN, Pascale ULiege; TOUSSAINT, Françoise ULiege; HAYETTE, Marie-Pierre ULiege et al

Poster (2012, April)

The aim of this study was to evaluate three commercial automated immunoassays for the serological diagnosis of M. pneumoniae infection.

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See detailExpansion of the main auroral oval at Jupiter : evidence for Io’s control over the Jovian magnetosphere
Bonfond, Bertrand ULiege; Grodent, Denis ULiege; Gérard, Jean-Claude ULiege et al

Poster (2012, April)

In spring 2007, New Horizons' Jupiter fly-by provided a unique opportunity for the largest observation campaign dedicated to the Jovian aurora ever carried out by the Hubble Space Telescope. UV images of ... [more ▼]

In spring 2007, New Horizons' Jupiter fly-by provided a unique opportunity for the largest observation campaign dedicated to the Jovian aurora ever carried out by the Hubble Space Telescope. UV images of the aurora have been acquired on a quasi-daily basis from mid-February to mid-June 2007. Polar projection of the auroral emissions clearly show a continuous long-term expansion of main oval additionally to day by day variations. The main oval moved so much that the Ganymede footprint, which is usually located equatorward of the main emissions, has even been observed inside of it. Simultaneously, the occurrence rate of large equatorward isolated auroral features increased over the season. These emission patches are generally attributed to injections of depleted flux tubes. On 6th June, one of these features exceptionally moved down to the Io footpath. The Io footprint seemed to disappear while the footprint moved through this patch of emission. This disappearance is a unique case among all the UV images of the aurora acquired during the last 12 years. We suggest that all these changes seen in the Jovian aurora are evidence for a major reconfiguration of the magnetosphere induced by increased volcanic activity on Io. Indeed, New Horizons observed particularly intense activity from the Tvashtar volcano in late February 2007. Moreover, sodium cloud brightening caused by volcanic outbursts have also been seen in late May 2007. According to our interpretation, repeated volcanic outbursts beefed up the plasma torus density and its mass outflow rate. This caused the corotation breakdown boundary to migrate closer to Jupiter. Consequently, the main auroral oval moved equatorward. As heavy flux tubes move outward, sparsely filled ones should be injected into the inner magnetosphere in order to conserve the magnetic flux in this region. This phenomenon could explain the large number of injection signatures observed in May-June 2007. Such a cloud of depleted flux tubes probably disrupted the Io-magnetosphere interaction, leading to an abnormally faint Io footprint. [less ▲]

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See detailThe Venus nitric oxide nightglow vertical distribution : update, new features and modelling
Stiepen, Arnaud ULiege; Gérard, Jean-Claude ULiege; Soret, Lauriane ULiege et al

Poster (2012, April)

Ultraviolet (UV) spectra of the delta (190-240 nm) and gamma (225-270 nm) bands of the nitric oxide (NO) molecule have been measured in the atmosphere of the Venus night side with the Spectroscopy for ... [more ▼]

Ultraviolet (UV) spectra of the delta (190-240 nm) and gamma (225-270 nm) bands of the nitric oxide (NO) molecule have been measured in the atmosphere of the Venus night side with the Spectroscopy for Investigation of Characteristics of the Atmosphere of Venus (SPICAV) instrument on board Venus Express (VEX). Excited NO molecules on the night side of the planet find their source in the radiative recombinaison of O(3P) and N(4S) atoms produced on the dayside by Extreme Ultraviolet (EUV) solar photons that cause photodissociation of CO2 and N2 molecules. We analyse with an improved statistics the behaviour of the vertical emission profile of the NO nightglow. We also present a method used to retrieve and analyse the volume emission rate. We describe the dependence of the vertical distribution with latitude and local time and its variability. New features in the vertical distribution of the NO emission such as double peaks are also exhibited. Furthermore, we use a one-dimensional chemical-diffusive model to compare the major features of the calculated O2 1.27 microm and NO UV emissions profiles with those observed with SPICAV. [less ▲]

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See detailAllergenic properties of the house dust mite protease Der p 3
Bouaziz, Ahlem ULiege

Poster (2012, April)

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See detailShaft sealing issue in CO2 storage sites
Dieudonné, Anne-Catherine ULiege; Charlier, Robert ULiege; Collin, Frédéric ULiege

Poster (2012, April)

Detailed reference viewed: 82 (24 ULiège)
See detailInorganic carbon dynamics in coastal arctic sea ice and related air-ice CO2 exchanges
Geilfus, Nicolas-Xavier ULiege; Tison, J.-L.; Carnat, G. et al

Poster (2012, April)

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See detailTimbre description of the sound of air-treatment systems for predicting acoustic confort
Minard, Antoine; Billon, Alexis ULiege; Goujard, B. et al

Poster (2012, April)

Detailed reference viewed: 27 (5 ULiège)
See detailMagnetic hybrid materials for triggered drug delivery and optical properties of intraocular lens
Liu, Ji ULiege; Hurtgen, Marie ULiege; Mornet, Stephan et al

Poster (2012, March 30)

Stimuli-responsive nano-materials have been playing increasingly important roles in the biomedical field due to their ability to alter the physico-chemical properties in response to external stimuli, such ... [more ▼]

Stimuli-responsive nano-materials have been playing increasingly important roles in the biomedical field due to their ability to alter the physico-chemical properties in response to external stimuli, such as temperature, pH, ionic strength, magnetic field, etc. Nanohybrids bearing an inorganic core and thermo-responsive polymer shell are particularly applicable in target delivery and controlled drug release. Poly (N-vinylcaprolactam) (PNVCL) and its copolymers, exhibiting lower critical solution temperature (LCST) where the transition between hydrophilic and hydrophobic state happens, is one of the optimal choices for this core/shell structure. Here we present the in-situ fabrication of thermo-responsive gold nanohybrids coated with a novel poly (vinyl alcohol)-b-poly (N-vinylcaprolactam) (PVOH-b-PNVCL) block copolymers, which were prepared by the cobalt-mediated radical polymerization (CMRP). The interaction between both PVOH and PNVCL segments and gold nanoparticles was confirmed by FT-IR spectra. A sharp thermo-induced phase transition with a good reversibility upon change in temperature was detected by DLS and UV/vis spectrometer. Furthermore, the presence of hydrophilic PVOH moieties endows the gold nanohybrids with improved colloidal stability above LCST without any flocculation detected. This kind of gold nanohybrids can be envisaged as a new drug delivery vehicle. [less ▲]

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See detailDisulfide bond scrambling in partially reduced and alkylated peptides revealed by Ion Mobility Mass Spectrometry
Echterbille, Julien ULiege; Quinton, Loïc ULiege; De Pauw, Edwin ULiege

Poster (2012, March 29)

Animal venoms are mainly composed of peptide toxins, which are highly structured by many disulfide bridges. In these toxins, disulfides play different major roles such as increasing the toxins efficiency ... [more ▼]

Animal venoms are mainly composed of peptide toxins, which are highly structured by many disulfide bridges. In these toxins, disulfides play different major roles such as increasing the toxins efficiency by lowering their immunogenicity or providing the adequate conformation to efficiently bind to the biological receptor. Peptide sequencing followed by determination of the cysteine pairings is still challenging and, therefore, an important step in structural analysis. This work was, in its beginning, focused on the development of ion mobility (IMS) based methodology used to assign disulfides. The strategy relies on the analysis of partially reduced/alkylated disulfide containing peptides. The resulting mixture is analyzed by ion mobility, followed by MS/MS acquisition on each mobility resolved species. Surprisingly, first investigations revealed, after partial reduction, a disulfide rearrangement phenomenon. Indeed, some of the cystein pairings were not those expected to be. These experiments were conducted on ¿-CnI and ¿-GI toxins purified from the venoms of Conus consors and Conus geographus marine snails, respectively. Each toxin contains four cysteines linked together with two disulfide bridges. Peptides were partially reduced by an excess of dithiothreitol and then alkylated by a large excess of iodoacetamide. The resulting mixture was purified on a microcolumn before being analyzed by nanoESI-Synapt-G2. Fragmentation was performed after the mobility cell, to obtain specific fragments of each species. Each toxin partially reduced/alkylated results, theoretically, in a mixture of fully oxidized (two disulfides oxidized), fully reduced (two disulfides reduced) and partially reduced forms (one of the two disulfides reduced). Thanks to the mass shift created by the alkylation, an isolation of the species which m/z ratio corresponds to one disulfide reduced and alkylated has been done in the quadrupole before the mobility separation. The arrival time distribution of triply charged ions reveals the presence of different species (4 in the case of ¿-GI and 2 for ¿-CnI), characterized by different relative cross sections in the gas-phase. As ion mobility resolved species give characteristic fragments upon fragmentation (after IMS), we were able to identify a scrambling of the disulfides (isomerization). In simple words, other disulfide bonds than expected ones were characterized. We suppose that the scrambling phenomenon occurs in solution,during the reduction step, since the alkylation cannot avoid rearrangement. The method is now being applied to more complex systems containing 3 or 4 disulfide bridges. The influence of the charge state on the mobility separation is systematically analyzed in terms of structural implications. [less ▲]

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See detail1D many-body quantum transport of Bose-Einstein condensates : a truncated Wigner approach
Dujardin, Julien ULiege; Argüelles Parra, Arturo ULiege; Schlagheck, Peter ULiege

Poster (2012, March 29)

We calculate the transport properties of an ultracold gas of Bose- Einstein condensed atoms that is coupled from a magnetic trap into a one-dimensional waveguide. A central aim of such guided atom lasers ... [more ▼]

We calculate the transport properties of an ultracold gas of Bose- Einstein condensed atoms that is coupled from a magnetic trap into a one-dimensional waveguide. A central aim of such guided atom lasers is to study the role of atom-atom interaction in many-body transport processes across finite scattering regions within the waveguide resembling tunnel junctions and quantum dots. Our numerical approach to solve this many-body scattering problem is based on a home-made theory where the boundaries of the system are simulating an open system enabling us to calulate observable in the steady-state regime such as density, current, transmission. The results are compared to the mean-field regime. [less ▲]

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See detailAbundance estimates of southern right whales (Eubalaena australis) in Bahía San Antonio, Patagonia, Argentina
Vermeulen, Els ULiege; Cammareri, Alejandro

Poster (2012, March 27)

Aerial surveys were conducted to estimate the abundance of southern right whales in Bahía San Antonio, a bay located in the north-western region of the San Matías Gulf (40°50¿S 64°50¿W), Rio Negro ... [more ▼]

Aerial surveys were conducted to estimate the abundance of southern right whales in Bahía San Antonio, a bay located in the north-western region of the San Matías Gulf (40°50¿S 64°50¿W), Rio Negro, Patagonia Argentina. The transect for the realization of aerial surveys was designed using the program DISTANCE 6.0 and consisted out of 14 North-South (up to S 40.9°) parallel transect lines with a 2.5km separation, covering a total surface of 418km² (mean coverage probability=0.78). Transect length was chosen according to the safety restrictions of the pilot. Surveys were conducted in good weather conditions and calm sea state (Beaufort 3 or less) using a high-wing Cessna 152 with a flat window. Due to the small size of the aircraft, only one person could fly besides the pilot; observations where therefore made from one side only. Average speed and height of the aircraft was kept constant over the surveys at 90kn (166km/h) and 700ft (213m) respectively. When a group of southern right whales was sighted, data were taken on location (using a Garmin GPSmap 60csx), time and group size. The downward angle to the group perpendicular to the aircraft¿s track was then measured using a hand-held clinometer (Suunto PM5/360PC). Perpendicular distances were calculated by the means of trigonometry using the aircraft¿s altitude and the declination angle to the sighting. Due the flat windows of the aircraft a left truncation distance was set at 150m. Perpendicular distances were also right-truncated at 10% of the observations. The uniform cosine model was chosen in the view of the minimum AIC to model the detection function of southern right whales in the study area. The estimates of g(0)=(s+t)/(s+d) resulted in a correction factor of 0.392 ± 0.456 In total, 7 aerial surveys were conducted in the beginning of August and September 2009, September, October and November 2010, and August, September 2011, resulting in a total flight time effort of 12.4h. In total, 200 whales were seen in 119 whale groups, equally distributed over the entire bay. Group sizes ranged between 1-5 animals with a mean group size of 1.7 animals (SD=0.83). Results show a peak of averagely 136 ± 63 whales in the bay during the month of September, with less than half the amount of whales present in adjacent months. September being the peak month for right whale presence accords to data from other regions in the country. The aerial surveys resulted in the first estimates of southern right whale abundance in this north Patagonian bay and indicate a marked peak during September. Nevertheless, data obtained over the other months remain scarce. More aerial surveys should be conducted to accurately determine the evolution of southern right whale abundance in the study area. [less ▲]

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See detailMicrostructure characterisation of nanocomposite polymeric foams by X-ray microtomography
Plougonven, Erwan ULiege; Detrembleur, Christophe ULiege; Tran, Minh Phuong ULiege et al

Poster (2012, March 26)

Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon ... [more ▼]

Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon nanotubes inside a polymer matrix shows exceptional electromagnetic interference shielding effectiveness when foamed. However, the effective properties of such materials strongly depend on the shape and topology of the microstructural cells. An accurate method for investigating the cellular microstructure is X-ray microtomography (XRμT), for it is non-destructive, and it provides 3D geometric information. Although it cannot be used to observe nanofiller dispersion, it has a strong potential for cell structure characterisation. In order to reduce the need for trial and error in tailoring these materials, our objective is to quantify, using XRμT, cellular microstructure, for two different types of foaming procedures, namely supercritical CO2 batch foaming and freeze drying, to be able to establish a link between the structure and its shielding effectiveness. The main difficulty stems from the type of material being studied: it is light, therefore hardly absorbs X-rays, cell size is small compared to the resolution capacity of the tomograph, and cell wall thickness is extremely thin in some cases, making them very hard to discern in the images. For these reasons, common image analysis tools for identifying and delimiting objects in an image prove impractical. We propose an original method that uses the 3D autocorrelation function of the tomograms to determine statistical information from these images, such as average cell size and anisotropy, without the need to binarise and segment the images. [less ▲]

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See detailImpact of the alkyl chain carbon atom number of uronic acid-derivative surfactants on interfacial and colloidal systems
Razafindralambo, Hary ULiege; Richard, Gaetan ULiege; Laurent, Pascal ULiege et al

Poster (2012, March 26)

Uronic acid-derivative surfactants represent today an attractive group of Carbohydrate-based amphiphilic compounds for both fundamental and practical reasons [1,2]. In the continuity of our screening of ... [more ▼]

Uronic acid-derivative surfactants represent today an attractive group of Carbohydrate-based amphiphilic compounds for both fundamental and practical reasons [1,2]. In the continuity of our screening of their basic properties, and the approach in establishing relationships between their structure and properties [3,4], the impact of the alkyl chain carbon atom number on interfacial and colloidal systems has been systematically investigated. In this communication, the effect of odd-even number of carbon atom from C11 to C18, and the particular behavior of long chain derivatives on interfacial properties, as well as their impact on the formation and stability of colloidal systems, are presented. Experimental approaches have been conducted by investigating their properties and behaviors at gas-liquid and liquid-liquid under dynamic, equilibrium, and oscillatory interfacial conditions. Results are completed by and discussed with single molecule models established by theoretical approaches. [1] Razafindralambo, H.;Blecker, C.; Paquot, M., Screening of Basic Properties of Amphiphilic Molecular Structures for Colloidal System Formation and Stability. In Amphiphiles: Molecular Assembly and Applications, American Chemical Society: 2011; Vol. 1070, pp 53-66. [2] Laurent, P.; Razafindralambo, H.; Wathelet, B.; Blecker, C.; Wathelet, J.; Paquot, M., Synthesis and Surface-Active Properties of Uronic Amide Derivatives, Surfactants from Renewable Organic Raw Materials. J. Surfactants Deterg. 2011, 14, 51-63. [3] Razafindralambo, H.; Richel, A.; Wathelet, B.; Blecker, C.; Wathelet, J.; Brasseur, R.; Lins, L.; Miñones, J.; Paquot, M., Monolayer properties of uronic acid bicatenary derivatives at the air-water interface: effect of hydroxyl group stereochemistry evidenced by experimental and computational approaches. Physical chemistry chemical physics: PCCP 2011, 13 15291-15298. [4] Razafindralambo, H.; Blecker, C.; Mezdour, S.; Deroanne, C.; Crowet, J.; Brasseur,R.; Lins, L.; Paquot, M., Impacts of the Carbonyl Group Location of Ester Bond on Interfacial Properties of Sugar-Based Surfactants: Experimental and Computational Evidences. The journal of physical chemistry. B 2009, 113, 8872-8877. [less ▲]

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See detailSeasonal Variation in Abundance and Time-Budget of Bottlenose Dolphins (Tursiops truncatus) in Bahía San Antonio, Patagonia, Argentina
Vermeulen, Els ULiege; Cammareri, Alejandro; Holsbeek, Ludo et al

Poster (2012, March 26)

The abundance and time-budget of bottlenose dolphins (Tursiops truncatus) was assessed in Bahía San Antonio, Patagonia (Argentina) in the years 2009 and 2010. A total of 366.4 boat-based survey hours ... [more ▼]

The abundance and time-budget of bottlenose dolphins (Tursiops truncatus) was assessed in Bahía San Antonio, Patagonia (Argentina) in the years 2009 and 2010. A total of 366.4 boat-based survey hours resulted in 64 contact hours with a total of 88 dolphin groups. Mark-recapture abundance estimations, based on 63 identified dolphins, resulted in a corrected maximum estimate of 97 and 83 individuals during winter, and a minimum of 34 and 38 individuals during autumn of 2009 and 2010 respectively. Between 25% and 68% of the population consisted of unidentifiable individuals depending on the season, indicating the high presence of juveniles and calves. Behavioural data indicated that the dolphin¿s time-budget consisted mainly out of resting and feeding, variable over the seasons. Dolphins increased their time feeding and socializing during winter and spring, whereas feeding dropped to a minimum in autumn. During summer, the dolphins spent up to 46% of their time diving, a behaviour presumably associated with a tail out/peduncle-dive foraging strategy. Based on these data, we assume more prey availability during winter and spring (main food source being pelagic fish) and a notable decrease in prey availability during summer with benthic prey species being the main food source. In autumn, even less prey items might be available. Furthermore, the increase in social behaviour during winter and spring combined with a peak in the presence of calves during these seasons, suggests the existence of a mating and calving season. These estimates of abundance are in line with the sizes of other coastal populations of bottlenose dolphins elsewhere in the world, and fit the occupancy patterns described for other coastal areas with small resident communities. The study furthers suggests that dolphins specifically use the study area to rest and feed, and to give birth and raise their young, specifically during winter and spring. [less ▲]

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See detailAdsorption kinetics of total proteose peptone fractions at the air-water and n-dodecane-water interface
Karamoko, Gaoussou ULiege; Razafindralambo, Hary ULiege; Danthine, Sabine ULiege et al

Poster (2012, March 26)

Surface properties of whey proteins are essential in applications requiring oil emulsification in an aqueous phase, or foam structure formation [1]. The adsorption at interfaces constitutes then an ... [more ▼]

Surface properties of whey proteins are essential in applications requiring oil emulsification in an aqueous phase, or foam structure formation [1]. The adsorption at interfaces constitutes then an approach of the physico-chemical mechanisms of foaming and emulsifying properties. Proteose-peptone is the minor fraction of whey's protein, thermoresistant and acid-soluble protein fraction extracted from milk [2], and known for its interesting surface properties [3]. In this study, the TPP fractions were extracted from skimmed milk UHT (milk TPP) and whey protein concentrate (WPC TPP). Their adsorption kinetics at the air-water and n-dodecane-water interfaces was investigated by the drop volume tensiometer method. Protein solutions of 1% (w/v) were <br />characterized under dynamic condition at various pH (4.0; native pH 4.67- 4.70 and 7.0). Milk TPP showed the lowest values as well as a faster reduction in surface tension at both considered interfaces. Therefore, TPP were found to e effective as surfactants. These results let us to presage good emulsifying and foaming properties of milk TPP compared to WPC TPP. The considerable influence of pH and extraction source on proteose-peptone's interfacial property have been highlighted. [less ▲]

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See detailWATER-BORNE EMERGING ZOONOSE? CASE REPORT ON ERYSIPELAS (ERYSIPELOTHRIX RHUSIOPATHIAE) IN HARBOUR PORPOISES (PHOCOENA PHOCOENA) AND HARBOUR SEAL (PHOCA VITULINA).
Boseret, Géraldine ULiege; Saegerman, Claude ULiege; Mainil, Jacques ULiege et al

Poster (2012, March 26)

An adult female harbour porpoise (Phocoena phocoena) and a juvenile male harbour seal (Phoca vitulina) have been found stranded dead on the Belgian coast in late 2001. As the two bodies were in good ... [more ▼]

An adult female harbour porpoise (Phocoena phocoena) and a juvenile male harbour seal (Phoca vitulina) have been found stranded dead on the Belgian coast in late 2001. As the two bodies were in good condition (CC = 2), necropsy and bacteriological analyses were performed as well as other postmortem investigations. Blood heart and organs (liver, digestive and respiratory tract, lungs, spleen, brain, kidneys) samples have been collected and analyzed. The porpoise showed evidence of septicaemia, and the seal presented lesions of acute enteritis. Pure and abundant growth of a small rod-shaped, Gram-labile bacterium was obtained aerobically and anaerobically on Columbia blood agar from heart blood, mouth, pharynx, lungs, intestine and anus of the porpoise, and from intestine, pharynx, mouth, nose and anus of the seal. The colonies were surrounded by a narrow zone of alpha-hemolysis. Catalase- and peroxydase-tests gave negative results. Rapid ID 32 Strepto (Biomérieux, France) sugar tests identified this isolate as Erysipelothrix rhusiopathiae. E. rhusiopathiae could be therefore considered as the cause of septicaemia on the porpoise as it was present in heart blood and internal organs, and could be associated primary or secondary with the enteritis reported on the seal as the bacterium was isolated in pure culture in the digestive tract. E. rhusiopathiae infections have been reported in captive dolphins and sea lions. This zoonotic pathogen is also involved in human local infections, like the “seal finger”, resulting from captive pinnipeds bites. However, it has not been so far described as systemic pathogens of wild cetaceans and pinnipeds. E. rhusiopathiae could be therefore considered as a potentially emergent pathogen which could have important repercussions on human health, particularly veterinarians, marine mammals rescue teams and zoos. [less ▲]

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See detailThe treatment of hypersexuality by cognitive behavioral therapy and virtual therapy
Cismaru, Adina; Andrianne, Robert ULiege; Triffaux, Jean-Marc ULiege

Poster (2012, March 24)

The sexual addiction is an abnormal sexual practice. It becomes the center of the subject’s existence, by depriving him from other investments and without bringing him real satisfaction. This research ... [more ▼]

The sexual addiction is an abnormal sexual practice. It becomes the center of the subject’s existence, by depriving him from other investments and without bringing him real satisfaction. This research project intends to explore the field of sexual addiction and a possible treatment for it. Several studies on sexual dysfunction treated with psychotherapy and virtual reality have shown that we obtain better results than if we use just the psychotherapy. [less ▲]

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See detailEnvironmental product declarations of mineral paving, a tool for sustainable product choices
Belboom, Sandra ULiege; Renzoni, Roberto ULiege; Tourneur, Francis ULiege et al

Poster (2012, March 22)

This study performs the environmental product declarations for three Belgian mineral products, two granite and one sandstone paving. Both main goals of this work were to obtain Belgian environmental ... [more ▼]

This study performs the environmental product declarations for three Belgian mineral products, two granite and one sandstone paving. Both main goals of this work were to obtain Belgian environmental product declarations to strengthen existing databases and to get similar information for their Asian counterparts. Indeed, competition with China for bluestone products and with India for sandstone pavement is very important due to very competitive prices despite their foreign origin and possibly higher induced environmental impacts. [less ▲]

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Peer Reviewed
See detailEtude IRM par tenseur de diffusion (DTI) des anomalies microstructurelles de la matière blanche dans la maladie de Parkinson
Coolen, Tim; Cremers, Julien ULiege; André, Elodie ULiege et al

Poster (2012, March 21)

Introduction L’imagerie par résonance magnétique (IRM) conventionnelle du cerveau est réputée normale dans la maladie de Parkinson (MP), mais l’essor récent de techniques avancées offre de nouvelles ... [more ▼]

Introduction L’imagerie par résonance magnétique (IRM) conventionnelle du cerveau est réputée normale dans la maladie de Parkinson (MP), mais l’essor récent de techniques avancées offre de nouvelles perspectives, notamment l’IRM par tenseur de diffusion (DTI). Les études précédentes en la matière, hétérogènes dans leurs méthodes, montrent des résultats discordants. Ici, nous avons utilisé la DTI pour rechercher, sans hypothèse a priori, la présence d’anomalies microstructurelles au sein des principaux tracti de matière blanche dans la MP. Méthodes Soixante et un volumes en pondération de diffusion ont été acquis avec un système Allegra 3T (Siemens, Erlangen, Allemagne) au moyen d’une séquence DTI doublement refocalisée (1) chez 27 patients parkinsoniens non déments (durée moyenne d’évolution après le diagnostic : 5 ± 4.2 ans) et 25 contrôles d’âge (MP: 68,7±8,4; C: 65,1±8,8) et de genre similaires. Pour chaque sujet, les valeurs d’anisotropie fractionnelle (FA) et de diffusivité moyenne (MD) ont été extraites à partir d’un modèle du tenseur obtenu au moyen du logiciel ExploreDTI (2) faisant appel à la méthode RESTORE (3). Nous avons ensuite utilisé le module TBSS (v1.2) du logiciel FSL (4) pour conformer les images des scalaires dans un espace tridimensionnel commun puis rechercher, voxel-par-voxel, des différences entre les 2 groupes au sein du squelette de la matière blanche. Les résultats obtenus à l’issue d’un test par permutations (N=10000) ont été corrigés pour des comparaisons multiples. Résultats L’analyse des cartes de FA montre des valeurs significativement (P<0.05) plus élevées chez les patients dans plusieurs régions (5): fibres sous-corticales péri-rolandiques droites, parties du faisceau arqué droit, fibres du faisceau longitudinal inférieur et /ou fronto-occipital inférieur droit, fibres sous-corticales préfrontales gauches, partie postérieure du genou corps calleux. La comparaison inverse ne révèle aucun résultat significatif ni l’analyse des cartes de MD. Conclusions Ces résultats sont en accord avec les modèles physiopathologiques selon lesquels le primum movens dans la MP se situe dans une dysfonction synaptique et axonale (6,7). Une augmentation des valeurs de FA de la matière blanche dans la MP est en contradiction avec la plupart mais pas toutes (8,9) les études précédentes. L’hypothèse d’une diminution relative des fibres de croisement dans ces régions chez les patients mérite d’être testée au moyen de méthodes d’acquisition et d’analyse plus élaborées. Références 1. Nagy Z, et al. Magn Reson Med 2008; 60(5):1256-1260. 2. Leemans A, et al. Proc Intl Soc Mag Reson Med 17 2009;3537. (Abstract). 3. Chang LC, et al. Magn Reson Med 2005;3(5):1088-1095. 4. Smith SM et al. Neuroimage 2006; 31(4):1487-1505. 5. Catani M, et al. Cortex 2008; 44(8):1105-1132. 6. Schulz-Schaeffer WJ. Acta Neuropathol 2010; 120(2):131-143. 7. Cheng HC, et al. Ann Neurol 2010; 67(6):715-725. 8. Tessa C, et al. AJNR 2008; 29(4):674-680. 9. Wang JJ et al. Radiology 2011; 261(1):210-217. Remerciements Ce travail est financé par le Fonds de la Recherche Scientifique (FNRS-FRS) de la Communauté Française de Belgique. [less ▲]

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See detailDoes Tight Glycemic Control positively impact on patient mortality?
Penning, Sophie ULiege; Le Compte, Aaron J.; Signal, Matthew et al

Poster (2012, March 20)

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See detailConstraints on the Parental Melts of Enriched Shergottites from Image Analysis and High Pressure Experiments
Collinet, Max ULiege; Medard, Etienne; Devouard, Bertrand et al

Poster (2012, March 20)

Martian basalts can be classified in at least two geochemically different families: enriched and depleted shergottites. Enriched shergottites are characterized by higher incompatible element ... [more ▼]

Martian basalts can be classified in at least two geochemically different families: enriched and depleted shergottites. Enriched shergottites are characterized by higher incompatible element concentrations and initial 87Sr/86Sr and lower initial 143Nd/144Nd and 176Hf/177Hf than depleted shergottites. It is now generally admitted that shergottites result from the melting of at least two distinct mantle reservoirs. Some of the olivine-phyric shergottites (either depleted or enriched), the most magnesian Martian basalts, could represent primitive melts, which are of considerable interest to constrain mantle sources. Two depleted olivine-phyric shergottites, Yamato 980459 and Nothwest Africa (NWA) 5789, are in equilibrium with their most magnesian olivine and their bulk rock compositions are inferred to represent primitive melts. Larkman Nunatak 06319 and NWA 1068, the most magnesian enriched basalts, have bulk Mg# that are too high to be in equilibrium with their olivine megacryst cores. Parental melt compositions have been estimated by subtracting the most magnesian olivine from the bulk rock composition, assuming that olivine megacrysts have partially accumulated. However, because this technique does not account for the actual petrography of these meteorites, we used image analysis to study these rocks history, reconstruct their parent magma and understand the nature of oli-vine megacrysts. Our image analysis results are supported by a series of high-pressure experiments performed on LAR 06319 bulk rock composition to test if it could represent a primitive melt, and understand its crystallization history. [less ▲]

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See detailInteractions of apamin with pore mutated SK3 channels
Dilly, Sébastien ULiege; Lamy, Cédric; Poncin, Sylvie et al

Poster (2012, March 16)

In the present work, we have tested the impact of the replacement of valine residues in the pore region of SK3 (520) by either an alanine or a phenylalanine residue in terms of the interactions of apamin ... [more ▼]

In the present work, we have tested the impact of the replacement of valine residues in the pore region of SK3 (520) by either an alanine or a phenylalanine residue in terms of the interactions of apamin with these mutants in comparison with the corresponding native channels. Replacing valine residue at position 520 of the SK3 channel by a phenylalanine significantly increased the sensitivity of the channel to be blocked by tetraethylammonium (TEA) as previously reported. Indeed, an aromatic residue, such as a phenylalanine or a tyrosine, is frequently found in the pore region of several potassium channels more sensitive to TEA than SK channels. We measured the affinity (Kd) of apamin in saturation experiments and studied SK currents in transfected cells using patch clamp techniques. In parallel, molecular modelling techniques were used to examine the impact of these local modifications on the interaction of apamin with the corresponding channels. The presence of a phenylalanine in the pore region of potassium channels led to a higher sensitivity for TEA by creating more hydrophobic interactions as found by the docking procedure. In the in vitro binding experiments, the phenylalanine mutant (SK3VF) displayed a very low affinity for apamin. In patch clamp experiments, the SK current was only very partially blocked by apamin in the SK3VF mutant. Furthermore, apamin displayed an affinity and a blocking activity for the alanine mutant close to that for the corresponding native channels. In conclusion, the presence of a bulky and hydrophobic residue at a position near the pore mouth of SK3 channels has a negative impact on their interactions with apamin. [less ▲]

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See detailApplication of mixed polygenic model to control for cryptic/genuine relatedness and population stratification.
Gusareva, Elena ULiege; Mahachie John, Jestinah ULiege; Isaacs, Aaron et al

Poster (2012, March 12)

In genome-wide association studies (GWAs), population stratification may cause inflated type I errors and overly-optimistic test results, when not properly corrected for. During the past decade, several ... [more ▼]

In genome-wide association studies (GWAs), population stratification may cause inflated type I errors and overly-optimistic test results, when not properly corrected for. During the past decade, several methods have been proposed for association testing in the presence of population stratification. Among these, principal components-based approaches are the most popular. Principal component analysis (PCA) allows data transformation to a new coordinate system such that the projection of the data along the first new coordinate (called the PC1) has the largest variance; the second PC has the second largest variance, and so on. In practice, two components are usually enough to adjust or to control for population stratification. They can easily be included in parametric association models as covariates. Despite the success of this strategy, there are still some caveats which need further attention. Among these are that principal component-based methods generally do not account for cryptic relatedness (kinship) between supposedly unrelated individuals, are not straightforwardly adapted to accommodate family-based designs or mixtures of families and unrelated individuals, and do not always take proper account of the trait under investigation. In this work, we present an easy-to-use alternative that addresses the aforementioned issues. For quantitative traits, we propose to first use the mixed polygenic model (possibly taking into account important non-genetic confounders as covariates), second to derive “polygenic” residuals from this model – hereby removing genomic kinship relationships, and third to consider these residuals as new traits in a classical genome-wide QTL analysis for “unrelated individuals”. The polygenic component of the aforementioned mixed polygenic model describes the contribution from multiple independently segregating genes, all having a small additive effect on the trait under investigation. Via an extensive simulation study, with various settings of population stratification and admixture, we show that this approach not only removes most of the “relatedness” between individuals (cryptic relatedness or known relatedness), but also removes most of the remaining substructures caused by population stratification or admixture. As a proof of concept, we demonstrate the efficiency of this robust method to control for population stratification on real-life genome-scale data from the SNP Health Association Resource (SHARe) Asthma Resource project (SHARP) (dbGaP accession number phs000166.v2.p1). We also provide leads to extend this method to dichotomous traits. [less ▲]

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See detailL'entomophagie basée sur la production locale d'insectes comestibles : sommes-nous prêts ?
Sablon, Ludovic ULiege; Alabi, Taofic Abdel Fabrice ULiege; Drugmand, Didier et al

Poster (2012, March 07)

Les perspectives d’évolution de la population mondiale annoncent un accroissement continu menant à un niveau supérieur à 9 milliards d’individus d’ici 2050. Cette augmentation correspondra inévitablement ... [more ▼]

Les perspectives d’évolution de la population mondiale annoncent un accroissement continu menant à un niveau supérieur à 9 milliards d’individus d’ici 2050. Cette augmentation correspondra inévitablement à des besoins alimentaires accrus au niveau mondial. Même si des spécificités en terme de produits consommés et de disponibilités – facilités de productions varient d’une région à une autre, la limitation des superficies à consacrer aux productions agricoles, notamment dans le cadre des ressources animales est une constante. Il ne sera pas possible d’assurer des productions animales conventionnelles pour fournir les ressources nécessaires aux populations humaines à venir. Bien que les produits animaux comme sources de nutriments doivent être développés, il s’agit maintenant de se tourner vers des filières alternatives pour maximiser les ressources végétales utilisées en élevage, tout en minimisant l’espace nécessaire et les effets sur l’environnement, notamment en terme de résidus et polluants potentiels. L'entomophagie, ou le fait de consommer des insectes comme source alimentaire d’origine animale, est connue et pratiquée dans de nombreuses régions du monde depuis des siècles. A chaque situation locale, une solution entomologique comme ressource alimentaire peut être appliquée : par la collecte dans la nature ou par le développement d’élevage d’espèces indigènes. Si l’entomophagie est peu appliquée en Europe, c’est sans doute d’une part lié à une absence dans nos mœurs alimentaires et d’autre part lié à la diversité et l’abondance d’autres ressources animales plus conventionnelles dans notre référentiel occidental. Au vu de l’état prévisionnel de la difficulté à produire les ressources alimentaires animales dans un futur proche, peut-être est-il temps de ne plus considérer les insectes comme uniquement nuisibles et négatifs mais plutôt comme de nouvelles espèces à développer par exemple dans des productions industrielles. Si la taille de ces animaux d’élevage reste réduite, leurs capacités de reproduction, leurs courtes durées de développement et les taux de conversion de la biomasse végétale utilisée comme aliments nous permettent d’envisager des unités de production de tonnes de biomasses animales. En plus de cet aspect quantitatif, plusieurs paramètres qualitatifs font de nombreux insectes des ressources utiles notamment en terme de protéines (et plus particulièrement en terme de composition en acides aminés plus rares) et de lipides (avec une majorité d’acides gras insaturés, des ratios omégas 3 et 6 intéressants, l’absence de cholestérol,…). Chez nous l'entomophagie est encore méconnue même si l'on peut constater un intérêt croissant notamment ces derniers mois. Entre curiosité gustative, éveil environnementaliste lié au faibles quantités de résidus liées à la production d’insectes vis-à-vis d’autres élevages, ou efficacité de transformation de ressources végétales limitées, de plus en plus de personnes souhaitent franchir le pas de ne plus regarder mais bien goûter de l’insecte. Dans le cadre de plusieurs études menées à Gembloux Agro-Bio Tech – Université de Liège ainsi qu’à l’Insectarium Jean Leclercq – Hexapoda à Waremme, certains aspects socio-culturels liés à une diversité de préparations à base d’insectes ont été investigués. Des tendances nettes sur les critères menant à l’acceptation ou le refus catégorique de manger de l’insectes ont ainsi été déterminés. Aussi, des formulations particulières peuvent également être ciblées pour favoriser l’appréciation de la dégustation d’insectes. Face au défi alimentaire du futur et à l'intérêt croissant pour l'entomophagie dans diverses régions dont l’Europe, l’élevage industriel d’insectes constitue une piste sérieuse pour fournir une part non négligeable de produits animaux afin d’assurer des ressources notamment de divers nutriments pour les populations humaines. Diverses recherches sont actuellement en cours tant sur les aspects techniques de production que sur les approches socio-culturelles. Nul doute que d’ici quelques années, entiers ou fractionnés les insectes feront partie de nos régimes et habitudes alimentaires. [less ▲]

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See detailHarmonized Growth Models and Simulations of the Evolution of Pure Stands of Norway spruce, Larches and Douglas-fir for Southern Belgium (Wallonia).
Perin, Jérôme ULiege; Ligot, Gauthier ULiege; Cerisier, François ULiege et al

Poster (2012, March 02)

In order to compare different silvicultural scenarios we have undertaken the development of a software that can simulate stand evolution in regards to their species composition, site characteristics and ... [more ▼]

In order to compare different silvicultural scenarios we have undertaken the development of a software that can simulate stand evolution in regards to their species composition, site characteristics and silvicultural operations. As a first approach we limited the scope of our work to even-aged and pure coniferous stands of the three most important coniferous species in Wallonia (southern part of Belgium): Norway spruce (Picea abies), douglas-fir (Pseudotsuga menziensii) and larches (Larix sp.). This presentation introduces carried out simulations and compare their outcomes. First we gathered data sets from the permanent regional inventory, from observation networks and experimental plots throughout Wallonia. Then we adjusted, for these species, harmonized and distance-independent models of dominant-height growth, tree diameter growth, mortality, and the distribution of tree diameter frequency before the first thinning. Next we implemented those models in the Capsis platform, and we simulated, for the three species, the evolution of virtual stands using different scenarios. On one hand the evolution was performed without thinning, and on the other hand we applied silvicultural scenarios that are commonly used in Wallonia. As a result these models highlight, for example, the great juvenile growth and sensibility to stands density of larches whereas Norway spruce behaves the opposite. Further work is however needed to predict and simulate wood quality and value. Once this will be achieved we will attempt to optimize silvicultural scenarios for the three species. [less ▲]

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See detailRecent disturbances in the Congo Basin : an anthracological contribution to vegetation reconstructions
Morin-Rivat, Julie ULiege; Hubau, Wannes; Gillet, Jean-François ULiege et al

Poster (2012, March 01)

In contrast to the well-known vegetation history of the northern hemisphere, few is known about past vegetation change in Central Africa. However, recent palaeoecological and biogeographical studies ... [more ▼]

In contrast to the well-known vegetation history of the northern hemisphere, few is known about past vegetation change in Central Africa. However, recent palaeoecological and biogeographical studies suggest that early human disturbances had a substantial influence on Central Africa vegetation patterns, particularly allowing the expansion of light-demanding species. This interesting hypothesis is the basis of one of the main research questions of the ERA-net BIODIVERSA CoForChange project: what was and is the relationship between (increasing) human activity and vegetation change? A recently developed protocol for the identification of ancient Central African charcoal fragments opened the door for vegetation reconstructions with a high spatial and taxonomical resolution. Therefore, we chose to study macro-charcoals from pedoanthracological profiles situated in N Congo and SE Camero0n. In total, 48 taxa were found in nine radiocarbon dated profiles. At the moment, three taxa have been identified down to species level. First, we found numerous fragments of Gilbertiodendron dewevrei under a current monodominant forest of G. dewevrei (40 cm depth, 1421-1327 cal. BP). This seems to confirm the presumption that monodominant forests of G. dewevrei, shade-bearer species, are relatively stable. Second, we evidenced the lack of Triplochiton scleroxylon charcoals under a T. scleroxylon stand, which could confirm the hypothesis of the recent nature of those stands. Finally, our results suggest that taxonomic diversity of charcoal findings in open canopy Marantaceae forests is greater than in dense forests. Pterocarpus soyauxii and Millettia drastica have been found under an open Marantaceae forest at 40 cm depth (1184-1055 cal. BP). The abundance of the light-demanding species P. soyauxii appears to be decreasing over time (levels 20 to 40 cm depth) to the benefit of giant herbs. Anthracology in Central Africa is on the rise and the first results of the CoForChange project are promising. More identifications will follow, resulting in a better understanding of the evolution of Central African forests. [less ▲]

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See detailCould current Pericopsis elata (Fabaceae) patches in southeastern Cameroon be reasonably linked to past anthropogenic activities ?
Bourland, Nils ULiege; Cerisier, François ULiege; Livingstone Smith, Alexandre et al

Poster (2012, March 01)

Pericopsis elata is one of the most valuable African timber species. This IUCN Red Listed tree suffers from a lack of regeneration, thus its current presence provokes questioning. Our work aimed at ... [more ▼]

Pericopsis elata is one of the most valuable African timber species. This IUCN Red Listed tree suffers from a lack of regeneration, thus its current presence provokes questioning. Our work aimed at understanding its origins so as to help securing its future. This study, lead away from engineering works, was conducted in four different sites located within the natural distribution area of the species and took into account the different growing conditions were the species occurs. Our observations were based on an analysis of charcoal elements and pottery fragments discovered in subsurface layers of soils as well as on current botanical and pedological surveys. Discovered evidence of past human activities led to the assumption that this part of the Congo Basin was much more inhabited than previously thought. Some of the results obtained for P. elata could apply for other long lived light demanding species growing in the same environment. [less ▲]

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See detailSTEAM EXPLOSION : PROCESS AND IMPACT ON LIGNOCELLULOSIC MATERIAL
Jacquet, Nicolas ULiege; Vanderghem, Caroline ULiege; Danthine, Sabine ULiege et al

Poster (2012, March 01)

Steam explosion is a thermomechanochemical process which allows the breakdown of lignocellulosic structural components by steam heating, hydrolysis of glycosidic bonds by organic acid formed during the ... [more ▼]

Steam explosion is a thermomechanochemical process which allows the breakdown of lignocellulosic structural components by steam heating, hydrolysis of glycosidic bonds by organic acid formed during the process and shearing forces due to the expansion of the moisture. The process is composed of two distinct stages: vapocracking and explosive decompression. Cumul effects of both phases include modification of the physical properties of the material (specific surface area, water retention capacities, color, cellulose crystallinity rate,…), hydrolysis of hemicellulosic components (mono and oligosaccharides released) and modification of the chemical structure of lignin. These effects permit the opening of lignocellulosic structures and increase the enzymatic hydrolysis rate of cellulose components in the aim to obtain fermentable sugars used in second generation biofuels process. [less ▲]

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See detailINFLUENCE OF STEAM EXPLOSION ON CRYSTALLINITY PROPERTIES OF PURE CELLULOSE FIBER
Jacquet, Nicolas ULiege; Vanderghem, Caroline ULiege; Danthine, Sabine ULiege et al

Poster (2012, March 01)

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct ... [more ▼]

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct stages: vapocracking and explosive decompression. The treatment intensities is determined by a severity factor, established by a correlation between temperature process and retention time. The results show that steam explosion treatment has an impact on the crystallinity properties of pure cellulose fiber. When the severity factor is below 5.2, an increase of the overall crystallinity of the samples is observed with the treatment intensities. For higher intensities, a significant thermal degradation of cellulose lead to an important change in substrate composition, which lead to a further decrease of cellulose crystallinity [less ▲]

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See detailHow pristine is the Congo Basin forest ? Some answers from Erythrophleum spp. (Caesalpiniaceae) and Pericopsis elata (Fabaceae) natural stands in southeastern Cameroon
Bourland, Nils ULiege; Livingstone Smith, Alexandre; Guion, Hélène ULiege et al

Poster (2012, March 01)

Erythrophleum spp. and Pericopsis elata are two important African timber taxa suffering from regeneration problems. Those light demanding trees could have established in openings made by past human ... [more ▼]

Erythrophleum spp. and Pericopsis elata are two important African timber taxa suffering from regeneration problems. Those light demanding trees could have established in openings made by past human activities (mainly slash-and-burn cultivation). Our study aimed at verifying this assumption in a 340,000 ha forest concession in southeastern Cameroon. Anthracological excavations were made alongside transects opened in patches where those taxa occur as well as the surrounding environment. Numerous charcoal elements and some pottery fragments were found in excavated soils in all studied sites. Charcoals and pieces of pottery were found within the first 100 and 50 cm of soil, respectively. According to radiocarbon dating conducted on charcoals and burnt seeds, fires occurred between 2,150 to 195 years BP. This finding was consistent with the analysis of archaeological materials decoration techniques. Our results revealed intense past anthropological activities in this area, seriously questioning the assumption of a pristine Congo Basin [less ▲]

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See detailSMALL SAMPLE SIZE CAPABILITY INDEX FOR ASSESSING VALIDITY OF ANALYTICAL METHODS
Rozet, Eric ULiege; Boulanger, B.; Ziemons, Eric ULiege et al

Poster (2012, March)

Analytical method’s capability evaluation can be a useful methodology to assess the fitness of purpose of these methods for their future routine application. However, care on how to compute the capability ... [more ▼]

Analytical method’s capability evaluation can be a useful methodology to assess the fitness of purpose of these methods for their future routine application. However, care on how to compute the capability indices has to be made. Indeed, the commonly used formulas to compute capability indices such as Cpk, will highly overestimate the true capability of the methods. Especially during methods validation or transfer, there are only few experiments performed and, using in these situations the commonly applied capability indices to declare a method as valid or as transferable to a receiving laboratory will conduct to inadequate decisions. In this work, an improved capability index, namely Cpk-tol and the corresponding estimator of proportion of non conforming results (tolCpk−π) is proposed. Through Monte-Carlo simulations, they have been shown to greatly increase the estimation of analytical methods capability in particular in low sample size situations as encountered during methods validation or transfer. Additionally, the usefulness of this capability index is illustrated through several case studies. [less ▲]

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See detailSituation épidémiologique du virus de l’hépatite E chez le sanglier (Sus scrofa) en Belgique
Thiry, Damien ULiege; Mauroy, Axel ULiege; Brochier, Bernard et al

Poster (2012, March)

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See detailEvaluating COSMO’s lake module (FLake) for an East-African lake using a comprehensive set of lake temperature profiles
Thiery, Wim; Martynov, Andrey; Darchambeau, François ULiege et al

Poster (2012, March)

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See detailAN INNOVATIVE APPROACH TO SELECT THE PREDICTION MODEL IN THE DEVELOPMENT OF NIR SPECTROSCOPIC METHODS
Ziemons, Eric ULiege; Mantanus, Jérôme ULiege; Rozet, Eric ULiege et al

Poster (2012, March)

Taking into account its non-invasive, non-destructive character and fast data acquisition, near infrared spectroscopy is more and more integrated in production processes to acquire analytical results ... [more ▼]

Taking into account its non-invasive, non-destructive character and fast data acquisition, near infrared spectroscopy is more and more integrated in production processes to acquire analytical results. Implementation of a NIR quantitative method is performed using an iterative heuristic approach that will ultimately build a model allowing the prediction of the concentration of the analyte of interest. In this context, the aim of the present study was to develop an innovative approach based on statistical tolerance intervals and the desirability index FMI (Fitting Model Index) to select the most appropriate prediction model from a list of candidate models instead of using conventional criteria such as R², RMSEC, RMSECV and RMSEP [1-2] without objective decision rules. This new approach is illustrated on different steps of a real pharmaceutical manufacturing process: water and Active Pharmaceutical Ingredient (API) determinations in pharmaceutical pellets. Variability sources such as production campaigns, batches, days and operators were introduced in the calibration and validation sets. Partial Least Square (PLS) regression on the calibration sets was performed to build prediction models of which the ability to quantify accurately was tested with the validation sets. Regarding the product specifications, the acceptance limits were set at 20% and 5%, for the moisture and API determination, respectively.As can be seen from Figure 1 and 2, this innovative approach based on the desirability index FMI of the accuracy profile enabled to build and select the most appropriate prediction model in full accordance with its very final goal, to quantify as accurately as possible the analytes of interest. [1] Hubert Ph. et al., J. Pharm. Biomed. Anal., 36, 2007, 579-586. [2] Rozet E. et al., Ana. Chim. Acta, 591, 2007, 239-247. [less ▲]

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See detailAnalysis of tobacco mainstream smoke by GC×GC TOFMS
Brokl, Michal ULiege; Bishop, Louise; Wright, Christopher et al

Poster (2012, March)

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See detailPilot Trial of STAR in Medical ICU
Fisk, Liam M.; Le Compte, Aaron J.; Shaw, Geoffrey M. et al

Poster (2012, March)

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See detailProtocol 3 : Belgian innovative projetcs to help community dwelling frail elderly
de Almeida Mello, J; Maggi, Patrick ULiege; Van Durme, T et al

Poster (2012, March)

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See detailOptimalisation d’une RT-PCR en temps réel pour la détection du virus de l’hépatite A
Zonta, William ULiege; Denayer, Sarah; Thiry, Etienne ULiege et al

Poster (2012, March)

En Belgique, l’incidence de l’hépatite A (HAV) est de 1,2 cas pour 100000 habitants. La population présente une faible immunité contre le HAV puisque seulement 50 % de la population possèdent des ... [more ▼]

En Belgique, l’incidence de l’hépatite A (HAV) est de 1,2 cas pour 100000 habitants. La population présente une faible immunité contre le HAV puisque seulement 50 % de la population possèdent des anticorps anti-HAV après l’âge de 30 ans. Ainsi un grand nombre d’individus reste susceptible de contracter une infection par le HAV. Les sources de contamination sont principalement le contact de personne à personne mais aussi la consommation d’aliments (crus ou « ready-to-eat) contaminés. Cette contamination peut provenir de l’eau d’irrigation pour les fruits et légumes, de l’eau de mer pour les mollusques bivalves ou de la manipulation de l’homme lors des différentes étapes entre la récolte et la vente du produit. Dans ce projet, une nouvelle RT-PCR spécifique pour la détection du HAV est développée et évaluée. Pour choisir de nouvelles amorces et sondes, des alignements sont réalisés avec les séquences de 19 souches de HAV (DNASTAR Lasergene) afin de cibler les régions les plus conservées du génome du HAV. Cinq nouveaux couples d’amorces ciblant plusieurs régions hautement conservées du génome de HAV ont été sélectionnés et testés à différentes températures d’hybridation et différentes concentrations en utilisant un agent se liant à l’ADN double brin (SYBR Green). Une sonde fluorescente, de type FAM, spécifique de l’amplicon délimité par le couple d’amorces fournissant les meilleurs résultats a été dessinée et utilisée avec la technologie d’hydrolyse de sonde à deux concentrations différentes. Des dilutions d’un facteur 10 de la suspension de la souche HM175 (HAV) ont été testées par le couple d’amorces et la sonde choisit pour établir ainsi une limite de détection. La spécificité du couple d’amorce choisit a été testé en présence de différents picornavirus et virus entériques et de 3 génotypes de HAV (IA, IB et IIIA). Le plasmide Sybricon019 et ses amorces spécifiques ont été utilisés comme contrôle interne d’amplification (IAC). Un contrôle positif a aussi été créé afin de s’assurer du fonctionnement correct de la PCR en temps réel. Parmi les 5 couples d’amorces sélectionnés, le couple HAV-F2/HAV-R2 permet d’obtenir les valeurs de Ct les plus faibles avec une concentration optimale de 300nM pour les amorces sens et anti-sens. Ce couple d’amorces cible la région VP1/VP3 du génome du HAV. Différentes températures d’hybridation ont été testées et la température la plus élevée (60°C) a été sélectionnée pour limiter le risque d’amplification aspécifique. Pour augmenter la spécificité, une sonde, HAV P2, spécifique de l’amplicon délimité par les amorces HAV-F2 et HAV-R2, est utilisée à la concentration de 250nM. Des dilutions d’un facteur 10 d’une suspension de HAV, 107 à 101 particules infectieuses par ml (déterminées par TCID50), donnent respectivement des valeurs de Ct de 19,2 à 38,4 et les dilutions de 100 à 10-2 particules infectieuses par ml ne donnent aucune amplification. La limite de détection est donc de 10 particules infectieuses par ml. La spécificité des amorces et de la sonde pour la détection du HAV est correcte puisque les trois génotypes de HAV, IA, IB et IIIA, ont été détectés alors que les différents picornavirus et virus entériques n’ont donné aucun signal fluorescent d’amplification. L’optimalisation d’un nouveau couple d’amorce et d’une sonde (HAV-F2, -R2 et -P2) ciblant une région hautement conservée du génome permet de détecter le virus HAV par RT-PCR. La région ciblée (VP1/VP3) diffère de la plupart des méthodes de détection de HAV par PCR en temps réelle décrites à ce jour. La seconde étape consiste à réaliser une série de tests dans différentes matrices alimentaires à risque (fruits de mer, fruits et légumes crus) dans le but de détecter des échantillons naturellement contaminés par le HAV. [less ▲]

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See detailRisques d'introduction des alphavirus responsables des encéphalites virales équines américaines en Belgique
De la Grandière de Noronha Cotta, Maria Ana ULiege; Dal Pozzo, Fabiana ULiege; Francis, Frédéric ULiege et al

Poster (2012, March)

Les virus transmis par des arthropodes hématophages (arbovirus) représentent une menace pour la santé animale et humaine en fonction de l’augmentation de l’émergence des arboviroses en dehors des ... [more ▼]

Les virus transmis par des arthropodes hématophages (arbovirus) représentent une menace pour la santé animale et humaine en fonction de l’augmentation de l’émergence des arboviroses en dehors des territoires endémiques. Les arbovirus étudiés ici appartiennent au genre Alphavirus et à la famille des Togaviridae, et sont composés d’un génome à ARN simple brin de polarité positive et enveloppés. Ces virus sont des pathogènes exotiques des équidés et causent des maladies graves chez l’homme lors d’épidémies. Les arbovirus présentent une épidémiologie complexe car ils se retrouvent au centre de l’interaction avec 5 composants : le virus, le vecteur, le réservoir, les espèces animales sensibles et l’environnement. Les voies d’introduction possible en Belgique de ces virus ont été investiguées ici en fonction des caractéristiques étiologiques et épidémiologiques de chaque virus. L’encéphalite équine de l’Est (EEEV) a un cycle complexe principal incluant les oiseaux et des moustiques comme Culiseta melanura. Les souches nord-américaines et sud-américaines peuvent être différenciées antigéniquement et génétiquement et ont des différences importantes dans leur cycle de transmission et au niveau de leur virulence. Le cycle naturel de l’EEEV se réalise chaque année dans des zones marécageuses et la migration d’oiseaux virémiques serait une hypothèse à sa réintroduction printanière annuelle. Certaines années, le virus peut être amplifié pendant ce cycle oiseaux-moustiques et le virus devient disponible à d’autres espèces de vecteurs qui font le pont entre les oiseaux et les humains ou d’autres mammifères comme les chevaux. Grâce à cette observation, l’EEEV a pu être isolé de vecteur comme Ochlerotatus sollicitans, Coquillettidia perturbans ou encore Culex pipiens et Aedes vexans qui sont deux espèces bien présentes en Europe. Le risque d’importation de l’EEEV semble peu élevé, elle pourrait se faire par l’intermédiaire du transport volontaire ou involontaire, légal ou illégal d’espèces réservoirs d’oiseaux comme des passériformes (ex : moineau domestique ou des oiseaux d’eau (ex : Egretta thula), des rongeurs comme le rat des cotonniers, des vertébrés ectothermiques comme les amphibiens (ex : Rana catesbeiana) ou des reptiles (ex : Agkistrodon piscivorus), ou encore par le transport accidentel de vecteurs compétents. L’encéphalite équine de l’Ouest (WEEV) est retrouvée dans l’Ouest de l’Amérique du Nord et en Amérique du Sud. Ce virus est un virus recombinant entre le Sindbis virus et l’EEEV. Le virus est inclus dans un cycle qui implique des passereaux et Culex tarsalis. Un deuxième cycle moins connu est rapporté et implique un lapin ou lièvre sauvage (Lepus europaeus) avec un moustique du genre Aedes. Comme pour l’EEEV, les humains et les chevaux ne développent pas une virémie suffisante pour infecter les moustiques et continuer le cycle. Le WEEV pourrait être introduit en Europe par différentes voies : les vecteurs arthropodes adultes infectés ou leurs œufs pour Aedes dorsalis, l’introduction d’oiseaux comme des passériformes ou des mammifères comme le lièvre sauvage virémiques. L’acquisition de la compétence d’un vecteur indigène local comme Culex pipiens ou Aedes dorsalis doit aussi être envisagée. Le groupe des virus de l’encéphalite équine vénézuélienne (VEEV) est sous-divisé en souches enzootiques et souches épizootiques qui utilisent le cheval comme hôte amplificateur. Les souches épizootiques sont opportunistes pour leur choix de vecteurs avec comme conséquence un large panel de vecteurs potentiels. L’introduction et l’établissement de ce virus en Belgique est possible via des moustiques infectés, des rongeurs comme le rat des cotonniers ou des oiseaux d’eau infectés, des chevaux et des hommes infectés qui sont des hôtes amplificateurs pour les souches épizootiques. En conclusion, il convient de faire la distinction entre l’introduction et l’établissement d’une infection de ces virus en Belgique. L’introduction peut se faire par l’intermédiaire de vecteurs insectes ou d’hôtes oiseaux ou mammifères comme les rongeurs ou encore le cheval et l’homme dans le cas du VEEV épizootique. Le maintien de l’infection nécessite la présence de vecteurs indigènes avec une compétence vectorielle ou de vecteurs compétents invasifs pour ces virus ainsi que la présence d’hôtes mammifères ou oiseaux phylogénétiquement apparentés à des espèces réservoirs dans les régions américaines où ces virus sont endémiques. [less ▲]

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See detailFoodborne Outbreak by a Recombinant Norovirus GIIg/1 Strain in a University Hospital in Munich
Hoffmann, Dieter; Mauroy, Axel ULiege; Foley, Brian et al

Poster (2012, March)

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See detailDelayed puberty in a girl due to an inactivating mutation of the LH receptor
FUDVOYE, Julie ULiege; PARENT, Anne-Simone ULiege; Bourguignon, Jean-Pierre ULiege

Poster (2012, March)

We report the case of a 46 XY patient with a disorder of sex differentiation (DSD) caused by an inactivating mutation of the LH receptor. Mutations of genes involved in hypothalamic-pituitary-gonadal ... [more ▼]

We report the case of a 46 XY patient with a disorder of sex differentiation (DSD) caused by an inactivating mutation of the LH receptor. Mutations of genes involved in hypothalamic-pituitary-gonadal function are rare but they provide an experience of nature for understanding the physiology and the pathophysiology of gonadotropins actions. There arise from correlation between the phenotypes and genotypes in those unique conditions. Management of this particular patient with no LH activity involves oestrogen replacement therapy to induce breast development together with a gonadectomy due to the risk of gonadoblastoma in streak gonads. [less ▲]

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See detailDu traumatisme obstétrical à la désinsertion trachéale.
Lecomte, Laurie; DEMEZ, Pierre ULiege; François, Anne et al

Poster (2012, March)

Les désinsertions trachéales traumatiques néonatales sont exceptionnelles. Elles sont généralement associées à une intubation compliquée, mais quelques descriptions ont été faites de ruptures trachéales ... [more ▼]

Les désinsertions trachéales traumatiques néonatales sont exceptionnelles. Elles sont généralement associées à une intubation compliquée, mais quelques descriptions ont été faites de ruptures trachéales partielles dans le décours d’un traumatisme obstétrical. Quelle que soit l’étiologie de la lésion trachéale, la prise en charge peut être conservatrice (par cicatrisation « simple » autour d’un tube entotrachéal) ou chirurgicale (par réparation des lésions). [less ▲]

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See detailAnthropisation des paysages: Structure spatiale, impact écologique, entropie
Vranken, Isabelle ULiege; Bogaert, Jan ULiege; Visser, Marjolein

Poster (2012, February 29)

Structural and thermodynamic applications of entropy related to spatial heterogeneity under growing anthropogenic pressure have been examined. 16 zones from classified LANDSAT TM scenes presenting ... [more ▼]

Structural and thermodynamic applications of entropy related to spatial heterogeneity under growing anthropogenic pressure have been examined. 16 zones from classified LANDSAT TM scenes presenting different anthropogenic effect intensities have been used. Anthropogenic effects, compositional and configurational entropy have been measured. The scatter plot shows bell curves with maximal entropy at intermediate anthropogenic effects. Distinction of natural and anthropogenic classes shows opposed tendencies according to class and compositional/configurational type. These results have been interpreted as spatial transformation processes, energy production and release, and linked to the habitat heterogeneity and intermediate disturbance hypotheses. [less ▲]

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See detailBiofuel by-product for poultry diets
Hissette, Mathias ULiege; Destain, Jacqueline ULiege; Thewis, André ULiege et al

Poster (2012, February 28)

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See detailViewing through the clouds in satellite images
Troupin, Charles ULiege; Barth, Alexander ULiege; Alvera Azcarate, Aïda ULiege et al

Poster (2012, February 24)

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See detailReconstruction of the long-term satellite-derived sea surface temperature in the South China Sea
Huynh, Thi Hong Ngu ULiege; Alvera Azcarate, Aïda ULiege; Barth, Alexander ULiege et al

Poster (2012, February 24)

The AVHRR (Advanced Very High Resolution Radiometer) sea surface temperature is very useful for researches in oceanography because of its high resolution. An AVHRR limitation is the high missing data ... [more ▼]

The AVHRR (Advanced Very High Resolution Radiometer) sea surface temperature is very useful for researches in oceanography because of its high resolution. An AVHRR limitation is the high missing data percentage due to cloud coverage. In the South China Sea, the average missing data is usually more than 80%, especially more than 95% in the region near the Borneo Island. In this study, we use DINEOF tool to reconstruct a daily night-time AVHRR data set with horizontal resolution of 4km spanning from 1989 to 2009. Besides, a comparison between the results and in situ data is shown. The EOF analysis shows that the first three modes explain about 95% of seasonal variability. [less ▲]

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See detailSupervised learning to tune simulated annealing for in silico protein structure prediction
Marcos Alvarez, Alejandro ULiege

Poster (2012, February 21)

Simulated annealing is a widely used stochastic optimization algorithm whose efficiency essentially depends on the proposal distribution used to generate the next search state at each step. We propose to ... [more ▼]

Simulated annealing is a widely used stochastic optimization algorithm whose efficiency essentially depends on the proposal distribution used to generate the next search state at each step. We propose to adapt this distribution to a family of parametric optimization problems by using supervised machine learning on a sample of search states derived from a set of typical runs of the algorithm over this family. We apply this idea in the context of in silico protein structure prediction. [less ▲]

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See detailImpact of glycerol and storage temperature on gluatathione concentration and physiological state of Pseudomonas fluorescens BTP1 freeze-dried
Mputu Kanyinda, Jean-Noël ULiege; Pierart, C.; Delvigne, Frank ULiege et al

Poster (2012, February 15)

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze ... [more ▼]

Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze-drying is a commonly used method to preserve bacteria. However, freeze-drying damages the cells, which results in loss of viability. Protective compounds are used to reduce loss of viability during process (freeze-drying and storage). In our study we used flow cytometry analysis to assess the physiological state in which cells are at the end of freeze-drying and Glutathione (GSH) was measured before and during storage. [less ▲]

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See detailOptiMIR: new tools for a more sustainable dairy sector
Goubau, Amaury ULiege

Poster (2012, February 10)

The OptiMIR project aims to improve the sustainability of the dairy sector by providing milk producers with new tools enabling them to manage the cow’s fertility, feeding, health, pollutants, and milk ... [more ▼]

The OptiMIR project aims to improve the sustainability of the dairy sector by providing milk producers with new tools enabling them to manage the cow’s fertility, feeding, health, pollutants, and milk quality. Data provided by milk recording organizations and recorded in different environments will be pooled and used to identify the links between animal status and milk composition changes. The entire MIR milk spectrum will be used as indicator of the cows’ status in order to 1) reduce production costs 2) give an access to high added value market and 3) reduce the environmental impact. OptiMIR is a 5 years project involving 5 research units, 11 milk recording organizations from Belgium, France, Germany, Ireland, Luxembourg, and United Kingdom. The project is co-financed by INTERREG IVB program. [less ▲]

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See detailVariability among morning and evening milk compositions during the lactation
Arnould, Valérie ULiege; Gengler, Nicolas ULiege; Soyeurt, Hélène ULiege

Poster (2012, February 10)

The main objective of this study was to analyze milking-to-milking variability of milk yield and milk composition (such as fat and protein percentages and somatic cell count). Additional objective was to ... [more ▼]

The main objective of this study was to analyze milking-to-milking variability of milk yield and milk composition (such as fat and protein percentages and somatic cell count). Additional objective was to extend this analyze to the milk fat composition. Milk samples (n=195.960) were collected from 29.636 cows in 491 Luxembourg farms and analyzed by MIR spectrometry. The milk contents of saturated, mono-, poly- and unsaturated fatty acids, and short, medium and long chain fatty acids were predicted from the recorded MIR spectral data. As expected, the milk composition and the milk fat composition, are affected by several factors as the milking period and the days in milk. In practice, using separately milk evening and milk morning could be interesting for cheese or butter production. [less ▲]

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See detail50 Years of contrasted residue management in an agricultural crop: impacts on the soil carbon budget and on heterotrophic respiration.
Buysse, Pauline ULiege; Roisin, Christian; Aubinet, Marc ULiege

Poster (2012, February 10)

This study aims to estimate the carbon (C) loss by soil heterotrophic respiration (SHR) in three contrasted residue management treatments (Residue Export, Farm Yard Manure addition and Residue Restitution ... [more ▼]

This study aims to estimate the carbon (C) loss by soil heterotrophic respiration (SHR) in three contrasted residue management treatments (Residue Export, Farm Yard Manure addition and Residue Restitution after harvest) through the establishment of soil C budgets, and to compare these estimations with field SHR measurements. The soil C budgets were calculated in each case on the basis of total soil organic C content and C input data compiled since the beginning of the experiment in Belgium, 50 years ago. SHR fluxes were measured in 2010 and 2011 to compare them with the budget-based estimates and to assess SHR sensitivity to temperature. The comparison suggested that the treatment receiving the largest C input does not necessarily sequestrate the most C or produce the largest CO2 fluxes. [less ▲]

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See detailUsing fatty acid contents in milk to improve fertility of dairy cows?
Bastin, Catherine ULiege; Berry, D.P.; Soyeurt, Hélène ULiege et al

Poster (2012, February 10)

Improving dairy cow fertility by means of genetic selection has become increasingly important over the last years in order to overcome the declining cow fertility. This study investigated whether the ... [more ▼]

Improving dairy cow fertility by means of genetic selection has become increasingly important over the last years in order to overcome the declining cow fertility. This study investigated whether the fatty acids profile in milk could be used as an early predictor of genetic merit for fertility. Genetic covariances among 17 fatty acid contents in milk and the number of days from calving to conception were estimated from 29,792 first-parity Holstein cows. Results substantiated the unfavorable relationship among fertility and body fat mobilization in early lactation. Also, about 75% of the genetic variability of fertility was explained by the variability in milk fatty acids profile over the lactation indicating that these traits could be used to supplement genetic evaluations for fertility. [less ▲]

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See detailAnthropogenic effect on landscapes: spatial structure, ecological impact, entropy
Vranken, Isabelle ULiege; Bogaert, Jan ULiege; Visser, Marjolein

Poster (2012, February 10)

Structural and thermodynamic applications of entropy related to spatial heterogeneity under growing anthropogenic pressure have been examined. 16 zones from classified LANDSAT TM scenes presenting ... [more ▼]

Structural and thermodynamic applications of entropy related to spatial heterogeneity under growing anthropogenic pressure have been examined. 16 zones from classified LANDSAT TM scenes presenting different anthropogenic effect intensities have been used. Anthropogenic effects, compositional and configurational entropy have been measured. The scatter plot shows bell curves with maximal entropy at intermediate anthropogenic effects. Distinction of natural and anthropogenic classes shows opposed tendencies according to class and compositional/configurational type. These results have been interpreted as spatial transformation processes, energy production and release, and linked to the habitat heterogeneity and intermediate disturbance hypotheses. [less ▲]

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See detailDistribution of Culicoides, bluetongue virus vectors, on Belgium farms
Zimmer, Jean-Yves ULiege; Losson, Bertrand ULiege; Saegerman, Claude ULiege et al

Poster (2012, February 10)

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See detailIntegration of external information into genetic evaluations by a Bayesian procedure
Vandenplas, Jérémie ULiege; Gengler, Nicolas ULiege

Poster (2012, February 10)

One of the most important theoretical assumptions of methods used to assess genetic values is that all available information has to be considered simultaneously to obtain unbiased estimates. However, the ... [more ▼]

One of the most important theoretical assumptions of methods used to assess genetic values is that all available information has to be considered simultaneously to obtain unbiased estimates. However, the widespread international exchange of genetic material and, more recently, the important development of the genomic selection lead to the coexistence of different genetic evaluations. Therefore, the blending of the different sources of information is necessary to achieve better prediction. Integration of external information into genetic evaluations by a Bayesian procedure can partially resolve the problem under some assumptions. Results from such a method that also avoids double counting among external animals are highly similar to those from a joint evaluation. [less ▲]

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See detailUse of Chrysoperla carnea larvae for biological control of immature stages of Leptinotarsa decemlineata
Sablon, Ludovic ULiege; Haubruge, Eric ULiege; Verheggen, François ULiege

Poster (2012, February 10)

In laboratory assays, we demonstrated predation of Chrysoperla carnea lacewing larvae against eggs, first and second larval instars of Colorado potato beetle (CPB). When looking at the daily consumption ... [more ▼]

In laboratory assays, we demonstrated predation of Chrysoperla carnea lacewing larvae against eggs, first and second larval instars of Colorado potato beetle (CPB). When looking at the daily consumption, we found that prey consumption by the third larval instar was 3-fold higher compared to the two first instars. Partial or total consumption of prey was also numbered. Different proportions of partial/total consumption were found and these depend on the lacewing larval stage. This study provides new perspectives for possible use of C. carnea as a biological agent to control CPB. Nevertheless, additional work has to be conducted under semi-natural and field to completely evaluate this predatory potential. [less ▲]

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See detailImplication of honeydew microflora in ant-aphid mutualism
Fischer, Christophe ULiege; Lognay, Georges ULiege; Haubruge, Eric ULiege et al

Poster (2012, February 10)

Some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of ... [more ▼]

Some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of view, this phe-nomenon has been well studied from decades. However, its chemistry and semiochemical mechanisms are still largely unknown. This study aims to identify semiochemicals involved in the establishment of this relation, using both chemical and behavioral approaches. Bioassays revealed that the great-est part of ant attraction toward aphid colonies is due to honeydew volatile compounds; enabling ant scouts to find more quickly aphid colonies and distantly recognize myrmecophilous species. Many of those VOCs seeming to have microbial origins, the main honeydew microorganisms have been isolated and their roles in VOCs production and ant attraction have been investigated. It appeared that honeydew microflora holds a key role in the establishment of ant-aphids mutualistic relationship. [less ▲]

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See detailCharacterization of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) overwintering sites
Fassotte, Bérénice ULiege; Durieux, Delphine ULiege; Haubruge, Eric ULiege et al

Poster (2012, February 10)

The invasive multicoloured Asian ladybird, Harmonia axyridis (Pallas), forms large aggregations inside dwellings to overwinter. In order to highlight the specific features of infested houses, we ... [more ▼]

The invasive multicoloured Asian ladybird, Harmonia axyridis (Pallas), forms large aggregations inside dwellings to overwinter. In order to highlight the specific features of infested houses, we investigated a large number of overwintering sites in Wallonia between 2007 and 2011. These sites were characterized through a survey sent to homeowners confronted to invasion problems. The results indicate that H. axyridis preferentially selects isolated brick houses with red or white fronts to take shelter. Aggregations are mostly located at the first floor, essentially inside south or west oriented rooms. Furthermore, ladybirds generally gathered into wooden windows frames facing south or west, and to a lesser extent, in the upper corners of walls presenting the same orientation. [less ▲]

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See detailHymenoptera community of pig carcasse in an urban biotope
Frederickx, Christine ULiege; Dekeirsschieter, Jessica ULiege; Verheggen, François ULiege et al

Poster (2012, February 10)

Most reports published in the field of forensic entomology studies are focused on Diptera pattern colonization and are neglecting Hymenoptera succession. Hymenoptera are part of the entomofaunal ... [more ▼]

Most reports published in the field of forensic entomology studies are focused on Diptera pattern colonization and are neglecting Hymenoptera succession. Hymenoptera are part of the entomofaunal colonization of a dead body as parasitoids of fly pupae. However, one should consider Hymenoptera parasitoids in a forensic entomology context to evaluate the time of death. Blowflies parasitoids may indeed be of particular importance as their time of attack is often restricted to a small, well-defined window of developmental time of the insect host. Because these parasitoids also interfere with developmental times of colonizing Diptera, a better understanding of their ecology is needed. [less ▲]

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See detailEvaluation of the genetic diversity of honey bees, Apis mellifera L. in the Walloon Region and selection of strains tolerant to the mite Varroa destructor Anderson and Trueman
Leclercq, Gil ULiege; Nguyen, Bach Kim ULiege; Gengler, Nicolas ULiege et al

Poster (2012, February 10)

For more than a decade, high losses of honey bee colonies have been noticed in several countries, including Belgium. Currently often the mite Varroa destructor is considered a main threat for beekeeping ... [more ▼]

For more than a decade, high losses of honey bee colonies have been noticed in several countries, including Belgium. Currently often the mite Varroa destructor is considered a main threat for beekeeping. In view of the inefficiency of the current chemical treatments, one of the solutions is to select honey bees tolerant to this parasite using genomic selection. To reach this objective the genetic diversity of honey bees needs first to be studied using mainly ‘Single Nucleotide Polymorphisms’ (SNP). Records and samples will be collected all over the Walloon Region in order to create an informative phenotypic and genomic data base that will be used for ‘Genome Wide Association Studies’ (GWAS) to detect associations between SNPs and tolerance, and to select bees tolerant to Varroa destructor. [less ▲]

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See detailVolatile organic compounds released by barley roots attract wireworms
Barsics, Fanny ULiege; Fiers, Marie ULiege; Haubruge, Eric ULiege et al

Poster (2012, February 10)

Wireworms are the soil dwelling larvae of click beetles and are pests of many crops worldwide. Alternatives to insecticide treatments are needed in order to develop integrated management strategies. Our ... [more ▼]

Wireworms are the soil dwelling larvae of click beetles and are pests of many crops worldwide. Alternatives to insecticide treatments are needed in order to develop integrated management strategies. Our work consists in elucidating the role of barley root-emitted volatile organic compounds on the orientation behaviour of Agriotes sordidus wireworms. Using a dual choice olfactometer we have evaluated the attractiveness of a variety of baits ranging from barley roots themselves to isolated root-emitted volatile organic compounds. Wireworms were significantly attracted towards most of the tested baits. Our results should be taken into account in varietal selection, in crop rotation, or in trapping systems aiming at the reduction of the populations of this pest. [less ▲]

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See detailRelationships between methane emissions of dairy cattle and farm management.
Vanrobays, Marie-Laure ULiege; Vanlierde, Amélie ULiege; Kandel, Purna Bhadra ULiege et al

Poster (2012, February 10)

Livestock is considered as an important contributor to global methane emissions, predominately due to methanogenesis from ruminants. Moreover, these emissions also represent major losses of energy for ... [more ▼]

Livestock is considered as an important contributor to global methane emissions, predominately due to methanogenesis from ruminants. Moreover, these emissions also represent major losses of energy for dairy cows and therefore are linked to production efficiency. The on-going development of predictive equations (e.g., from milk composition) would allow to relate methane emissions to farm management (e.g., nutrition, environment) on a large scale in the Walloon Region of Belgium. Finally, by acquiring improved knowledge of these relationships, contributions to mitigate methane emissions could be based on an improved management of dairy herds. [less ▲]

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Peer Reviewed
See detailAphid predators sampling in agrosystems in Belgium between 2009 and 2011
Vandereycken, Axel ULiege; Durieux, Delphine ULiege; Joie, Emilie ULiege et al

Poster (2012, February 10)

The Multicolored Asian ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae), was imported in 1997 in Belgium to control aphid populations. Few years ago after its introduction, this exotic ... [more ▼]

The Multicolored Asian ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae), was imported in 1997 in Belgium to control aphid populations. Few years ago after its introduction, this exotic insect was well adapted to temperate climate conditions and spread out all over ecosystems in Europe causing decline of other aphidophagous species. In arboreal habitats, H. axyridis is the most dominant Coccinellids but we are still lacking information about this occurrence in agrosystems. An aphidophages sampling between 2009 and 2011 was realized in four agrosystems such as broad bean, wheat, corn and potato. Nevertheless H. axyridis populations rise (5 times) from 2009 to 2011 in 2011, H. axyridis is the third most observed aphidophages after Episyrphus balteatus (De Geer) and Coccinella septempunctata (Linné). H. axyridis is the dominating species in corn with 70.83±6.60 individuals per 100m². [less ▲]

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Full Text
See detailToward a single definition of periurban areas? A review of terms, definitions and characteristics relating to the "urban" terminology.
Andre, Marie ULiege; Mahy, Grégory ULiege; Lejeune, Philippe ULiege et al

Poster (2012, February 10)

Remote sensing image interpretation and classification require a clear definition of what is considered as a rural, a periurban, a suburban or an urban area. Such a definition is also essential in order ... [more ▼]

Remote sensing image interpretation and classification require a clear definition of what is considered as a rural, a periurban, a suburban or an urban area. Such a definition is also essential in order to compare results of studies in periurban zones. Different terms, definitions and characteristics relating to these four concepts have been reviewed in the literature and compared according to different criteria like their type, citation frequency, strenghts and weaknesses, in the scope of a landscape evolution analysis. [less ▲]

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Full Text
See detailA new HPLC method to quantify alliin, the major sulfur compound in garlic
DETHIER, Bérénice ULiege; Laloux, Morgan ULiege; Hanon, Emilien et al

Poster (2012, February 10)

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized ... [more ▼]

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized. This process leads to two stereoisomers. Their HPLC separation was the aim of this work. The method described in the literature (amino column, detection at 210 nm) showed a resolution of 1,1 between the isomers. A second method was developed: the stationary phase was porous graphitic carbon and a water-ACN gradient was used for the elution. It provided a resolution of 3,2, was shorter and underwent the validation process. The method leads to satisfying results. The separation is excellent, and the validation criteria are fulfilled. This may be valuable for future research on garlic. [less ▲]

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See detailBanana Streak Virus activation by tissue culture in natural interspecific banana hybrids
NINTIJE, Pierre

Poster (2012, February 10)

Banana and plantains are important in Burundi as a staple food crop and source of income for farmers. Nevertheless, their productions are threatened by pathogens among which viruses are especially harmful ... [more ▼]

Banana and plantains are important in Burundi as a staple food crop and source of income for farmers. Nevertheless, their productions are threatened by pathogens among which viruses are especially harmful. Elimination procedures of viruses are mainly performed by banana tissue culture techniques (TC) giving good sanitation results for most bananas’ viruses. However, Banana Streak Virus (BSV) a pararetrovirus is activated by TC for banana with the B genome. Therefore, there is a risk of encouraging the emergence of BSV in Burundi by the distribution of TC products to growers. In this work, we studied the activation of BSV by tissue culture in 5 banana cultivars collected in Burundi and micropropagated in vitro. The BSV activation was demonstrated in 4 cultivars using molecular tools. [less ▲]

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See detailModelling realized niche of metallophyte species along copper and cobalt gradients on Katanga copper hills
Boisson, Sylvain ULiege; Seleck, Maxime ULiege; Guillaume, Arielle et al

Poster (2012, February 10)

In South Central Africa were identified more than 650 plant species tolerant to heavy metals, several of which endemic to Katanga and critically endangered by mining activities. These metallophyte are ... [more ▼]

In South Central Africa were identified more than 650 plant species tolerant to heavy metals, several of which endemic to Katanga and critically endangered by mining activities. These metallophyte are distributed over a hundred hills containing high copper and cobalt concentrations (20 to 10000 mg/kg for copper and 2 to 1000 mg/kg for cobalt). To understand the response of metallophyte to heavy metals, the ecological niches of 80 cupro-cobaltophytes were modeled with general additive models (GAM). Results show that (1) three groups of species were identified according to their optimum along a metal concentrations gradient and (2) a positive relationship exists between niche amplitude and optimum copper concentration. [less ▲]

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See detailImpact of cultivation practices on soil respiration
Dufranne, Delphine ULiege; Vancutsem, Françoise ULiege; Bodson, Bernard ULiege et al

Poster (2012, February 10)

Cultivation practices are known to induce a modification of soil organic matter quantity, quality and spatial distribution, which may impact dry matter decomposition kinetics. In order to bring answers to ... [more ▼]

Cultivation practices are known to induce a modification of soil organic matter quantity, quality and spatial distribution, which may impact dry matter decomposition kinetics. In order to bring answers to these questions, a multidisciplinary project (SOLRESIDUS) was set up by the University of Liege, Gembloux Agro-Bio Tech in collaboration with Walloon Agricultural Research Centre (CRA-W). The aim was to investigate the impact of cultivation practices (tillage and residue restitution) on crop growth, yield and environment, as well as on soil properties and on activities. In the present study, we focused on the impacts on soil respiration soil for 3 years. We will present our first results. [less ▲]

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