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See detailThe contribution of macroalgae to the assessment of the ecological quality of the rivers in Wallonia based on macrophytes indicator values in the British and French approaches.
Sossey Alaoui, Khadija ULg; Galoux, Daniel; Rosillon, Françis ULg

Poster (2009)

In accordance with the water Framework Directive (WFD, European Parliament & The Council of the European Union, 2000) defining the overall ecological status of rivers, many hundred sites were analysed in ... [more ▼]

In accordance with the water Framework Directive (WFD, European Parliament & The Council of the European Union, 2000) defining the overall ecological status of rivers, many hundred sites were analysed in the Walloon network. Within each sample, macroalgae data were gathered at species/or at genus level and the main water quality parameters were collected several times per year from 2005 to 2009. <br /><br />Seventeen species and genera cited in the French and English lists of macrophyte methods used to assess the ecological quality of rivers were considered. Within these sites, the ratio of the macroalgae among the contributory species was examined and the impact of these on the final scores of the River Macrophytes Nutrient index (United Kingdom, Wilby et al.,2006) and the biological macrophytic index in rivers (France, Haury et al., 2006) was analysed.At the same time, a ranking of these macroalgae along a trophy gradient has been established thanks to a principal component analysis of the physico-chemical parameters and a weighting of the species presence in a given waterbody by its abundance.The relationships between macroalgae scores and those found in France and in United Kingdom were studied using the Spearman rank correlation coefficient. <br /><br />Literature: <br /><br />European Parliament & The Council of the European Union, 2000. Directive 2000/60/EC of the European Parliament and of the Council establishing a framework for the Community action in the field of water policy. Official Journal of the European Communities 327: 1-72.Haury, J., M.-C. Peltre, M. Trémolières, J. Barbe, G. Thiébaut, I. Bernez, H. Daniel, P. Chatenet, G. Haan-Archipof, S. Muller, A. Dutartre, C. Laplace-Treyture, A. Cazaubon & E. Lambert-Servien, 2006. A new method to assess water trophy and organic pollution – the Macrophyte Biological Index for Rivers (IBMR): its application to different types of river and pollution. Hydrobiologia 570: 153-158. Willby, N., J. Pitt & G. Phillips, 2006. Summary of approach used in LEAFPACS for defining ecological quality of rivers and lakes using macrophyte composition. Draft Report January 2006. [less ▲]

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See detailOptimisation of blocked designs in fMRI studies
Maus, Bärbel ULg; van Breukelen, G. J. P.; Goebel, R. et al

Poster (2009)

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See detailEffect of phospholipids on surface properties of rapeseed oleosins at two pH
Vaca Medina, Guadalupe; Mouloungui, Zéphirin; Roiz, Julie ULg et al

Poster (2009)

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See detailResistance of pearlfishes to saponins
Maïté, Todesco; Parmentier, Eric ULg; Eeckhaut, Igor

Poster (2009)

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See detailGlass production in late antiquity
Van Wersch, Line ULg; Mathis, François ULg; Dupuis, Thomas ULg et al

Poster (2009)

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See detailAgonistic sounds in the clownfish Amphiprion clarkii: implication of the swimbladder in the sound-producing mechanism
Colleye, Orphal ULg; Nakamura, Masaru; Parmentier, Eric ULg

Poster (2009)

Clownfishes are aggressive fishes that use sound production to defend their anemone territory. It has been shown that they produce agonistic sounds using a jaw teeth snapping. At present, this mechanism ... [more ▼]

Clownfishes are aggressive fishes that use sound production to defend their anemone territory. It has been shown that they produce agonistic sounds using a jaw teeth snapping. At present, this mechanism has highlighted the onset of the sound but has not explained yet which structure is responsible for the sound modulation. Interestingly, some acoustic features such as dominant frequency and pulse duration are directly related to fish size. Such variations are linked to a morphological constraint. Also, the existent relationship between fish size and swimbladder size implies that the swimbladder might be involved in the sound production. Sound analyses in Amphiprion clarkii showed that the experimental filling of the swimbladder with physiological liquid (NaCl 9‰) significantly modified the acoustic features. The most striking changes were a significant increase in dominant frequency and a significant decrease in pulse duration. These observations highlighted the implication of the swimbladder in sound modulation. In clownfishes, dominant frequency and pulse duration are morphologically determined signals. The swimbladder appears to modulate these acoustic features by acting as a resonant chamber. [less ▲]

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See detailSensitivity analysis of a 3D diffusive drying model
Janas, Sébastien ULg; Deroanne Claude; Bera, François ULg

Poster (2009)

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See detailReal-time spatial analysis of root water uptake in rhizotrons
Lobet, Guillaume ULg; Javaux, M.; Pages, L. et al

Poster (2009)

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See detailEffects of crops on solute transport in undisturbed soil
Garré, Sarah ULg; Javaux, Mathieu; Vanderborght, Jan

Poster (2009)

Models predicting movement of surface applied chemicals incorporate knowledge on the water velocity field and moisture content distribution. Although the influence of root water uptake on solute transport ... [more ▼]

Models predicting movement of surface applied chemicals incorporate knowledge on the water velocity field and moisture content distribution. Although the influence of root water uptake on solute transport is commonly recognized as important, it has been studied sparsely. Yet, plants may take up a large part of the infiltrating water, thereby influencing the water flow pattern in the soil and concurrently solute transport processes. For this reason, experiments are required to investigate the relationship between plant root water uptake and flow field variability. The role of root water uptake on solute transport will be elucidated in two undisturbed soil columns. During three consecutive experiments, the influence of growing barley on tracer movement through a silty soil in two lysimeters will be followed. At the first stage, an inert tracer is put on the two bare lysimeters and leached with constant irrigation. As steady-state flow can be assumed, it is possible to follow the tracer movement in the column by ERT and to identify regions of preferential flow and solute transport parameters. During the second experiment, the tracer will be applied to mature barley grown in the lysimeters. Combining the information about the water content obtained with TDR with the relation between water content, soil solution salinity and bulk electrical conductivity, the soil solution salinity distribution can be derived from images of bulk electrical conductivity obtained with ERT. Root growth will be monitored using a minirhizotron. By comparing the transport parameters obtained after these two experiments, the effect of root water on the transport process can be quantified. When the columns are washed out and the barley is harvested, the third phase will be carried out under the same steady state flow conditions as in the first experiment to investigate the effect of dead roots on soil structure. [less ▲]

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See detailCan Nicotine become the remedy for the Pregnancy Induced Hypertension Syndrome?-A basic Research-
Sarun, Tuya; Tomimatsu, Takuji; Mimura, Kazuya et al

Poster (2009)

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See detailLocalization of Nopp140 within mammalian cells during interphase and mitosis
Thiry, Marc ULg; Cheutin, Thierry; Lamaye, Françoise et al

Poster (2009)

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See detailNicotine suppresses interleukin-6 production from vascular endothelial cells
Tomimatsu, Takuji; Sharentuya, Namuxila; Mimura, Kazuya et al

Poster (2009)

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See detailHydroxyl detection on Venus and Earth and implications for ozone
Migliorini; Piccioni, G.; Cardesin-Molino, A. et al

Poster (2009)

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes ... [more ▼]

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes are discussed. [less ▲]

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See detailThe Surface Ocean CO2 Atlas (SOCAT) - Coastal Regional group
Borges, Alberto ULg; Chen, A. T. C.; The SOCAT Regional group

Poster (2009)

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See detail4th International Symposium on Chemosynthesis-based Ecosystems
Ponsard, J; Cambon-Bonavita, M-A; Lepoint, G et al

Poster (2009)

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See detailAnalysis of several analytical method validation strategies
Rozet, Eric ULg; Bouabidi, A.; Bouklouze, A. et al

Poster (2009)

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See detailEtude exploratoire des changements émotionnels et comportementaux chez des patients ayant la sclérose en plaques
Belhadj, Soumaya; DELRUE, Gaël ULg; VOKAER, Mathieu et al

Poster (2009)

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See detailTotal Error-Based Criterion for Analytical Method Transfer
Rozet, Eric ULg; Dewe, W.; Boulanger, Bruno ULg et al

Poster (2009)

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See detailBAM and PM-IRRAS analyses of the alamethicin interfacial organization in phospholipid monolayers
Kouzayha, Achraf; Nasir, Mehmet Nail ULg; Besson, Françoise

Poster (2009)

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See detailUse of somatic hybridization to look for aromatic liness of Abies : preliminary results
Misson, Jean-Pierre; Thomas, Cynthia; Roblain, N. et al

Poster (2009)

Objectif : Associer les caractéristiques de croissance d’Abies nordmanniana aux propriétés aromatiques d’Abies balsamea Axes de recherche: - Hybridation somatique à partir de protoplastes d’Abies balsamea ... [more ▼]

Objectif : Associer les caractéristiques de croissance d’Abies nordmanniana aux propriétés aromatiques d’Abies balsamea Axes de recherche: - Hybridation somatique à partir de protoplastes d’Abies balsamea et Abies nordmanniana. - Détermination des profils aromatiques comme moyen de criblage. [less ▲]

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See detailA prospective randomized multicenter trial of darbepoetin-alfa and I.V. iron administration after autologous hematopoietic stem cell transplantation.
Beguin, Yves ULg; Maertens, J.; DE PRIJCK, Bernard ULg et al

Poster (2009)

We conducted a multicenter prospective randomized study analyzing the impact of darbepoetin alfa (DA) with or mithout i.v. iron on erythroid recovery after autologous HCT.

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See detailAntioxidant and cytotoxicity activity of essential oil grown Lavandula dentata
Imelouane, B.; El Bachiri, A.; Wathelet, Jean-Paul ULg et al

Poster (2009)

The present study describes the phytochemical profile Antioxidant and cytotoxicity activity of Lavandula dentata essential oil, collected in eastern Morocco (Taforalt). The sample of essential oil was ... [more ▼]

The present study describes the phytochemical profile Antioxidant and cytotoxicity activity of Lavandula dentata essential oil, collected in eastern Morocco (Taforalt). The sample of essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC–MS. The in vitro cytotoxicity of the oil on the cancer cell lines PC-3 (prostate), V79 (fibroblaste) and normal P388D1 (murine macrophage) was examined. We have shown that the oil had negligible cytotoxic effects against all cell lines tested. The oil was also found to possess antioxidant activity as demonstrated by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical method. Lavandula dentata essential oil has promising potentials for incorporation into various food, cosmetic and pharmaceutical products for which a natural aroma, colour, antioxidant and antimicrobial additive is desired. [less ▲]

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See detailStudy of Essential Oils of Some Plants from Algeria (Salvia officinalis, Thymus vulgaris and Ruta chalepensis)
Brada, M.; Achour, D.; Wathelet, Jean-Paul ULg et al

Poster (2009)

The essential oil of three plants (Salvia officinalis, Thymus vulgaris and Ruta chalepensis) collected from Chlef, in the north west of Algeria, was isolated by steam distillation and investigated by GC ... [more ▼]

The essential oil of three plants (Salvia officinalis, Thymus vulgaris and Ruta chalepensis) collected from Chlef, in the north west of Algeria, was isolated by steam distillation and investigated by GC and GC/MS. The yield of essential oil (g/100g) was 1.6% for Salvia officinalis, 2.6% for Thymus vulgaris and 1.1% for Ruta chalepensis. The Salvia officinalis was mainly composed of b-thujone (25%), camphor (23.6%), 1,8-cineole (21.5%), viridiflorol (9.5%) and a-thujone (6%). The main constituents of Thymus vulgaris were carvacrol (48.4%), g -terpinene (14.9%), p-cymene (14.7%) and thymol (5.6%) while 2-undecanone (83.2%) constituted the major component of Ruta chalepensis essential oil. [less ▲]

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See detailVariation in DNA methylation patterns of Phaseolus bean interspecific hybrids leads to abnormal embryo and plant development
Abid, Ghassen ULg; Muhovski, Yordan; Jacquemin, Jean-Marie et al

Poster (2009)

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See detailInfluence of drying methods on yield and chemical composition
Dahak, K.; Wathelet, Jean-Paul ULg; Chechouani, H. et al

Poster (2009)

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See detailPhysicochemical characterization of fat blends related to margarine formulation
Danthine, Sabine ULg; Fernando Munoz, J; Cavillot, V et al

Poster (2009)

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See detailEnrichment of Anhydrous Milk Fat in Polyunsatured Fatty Acid Residues
Aguedo, Mario ULg; Hanon, Emilien ULg; Danthine, Sabine ULg et al

Poster (2009)

Lipozyme TL IM was used in a solvent-free batch, microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ... [more ▼]

Lipozyme TL IM was used in a solvent-free batch, microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ternary blend. [less ▲]

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See detailTurtle cell cultures for nucleolar studies
Lamaye, Françoise; Thiry, Marc ULg

Poster (2009)

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See detailInteresterification of rapeseed oil with anhydrous milk fat and its stearin fraction
Giet, Jean-Michel ULg; Aguedo, Mario ULg; Hanon, Emilien ULg et al

Poster (2009)

The compositional changes occurring during the lipase-catalysed interesterification of AMF/rapeseed oil (RO) and AMF stearin fraction (AMFSF)/RO blends were described in the first part of this study. In ... [more ▼]

The compositional changes occurring during the lipase-catalysed interesterification of AMF/rapeseed oil (RO) and AMF stearin fraction (AMFSF)/RO blends were described in the first part of this study. In the present and second part are reported the resulting changes in physical properties, especially the melting behaviour through solid fat content (SFC), dropping point (DP) and fusion profiles by differential scanning calorimetry (DSC). [less ▲]

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See detailModelling and parameter estimation for heterogeneous cell populations
Waldherr, S; Hasenauer, J; Schliemann, Monica ULg et al

Poster (2009)

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See detailInteresterification of rapeseed oil with anhydrous milk fat and its stearin fraction
Aguedo, Mario ULg; Giet, Jean-Michel ULg; Hanon, Emilien ULg et al

Poster (2009)

Rapeseed oil (RO) (a choice source of unsaturation-rich residues) was used in the present study to enrich anhydrous milk fat (AMF) with unsaturated C18 fatty acids (FA) (oleic, linoleic and linolenic ... [more ▼]

Rapeseed oil (RO) (a choice source of unsaturation-rich residues) was used in the present study to enrich anhydrous milk fat (AMF) with unsaturated C18 fatty acids (FA) (oleic, linoleic and linolenic acids). Comparatively, one “harder” fraction of AMF underwent the same reaction. The physico-chemical properties modifications induced by the reaction were followed. The compositional changes are reported in this first part and the consequent physical modifications are presented in a second part. [less ▲]

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See detailFluctuation of sediment production during the last millennia in the Ardenne Massif (Belgium)
Houbrechts, Geoffrey ULg; Notebaert, Bastiaan; Petit, François ULg et al

Poster (2009)

In the Ardenne massif, floodplains have recorded fluctuations in the production of sediment and several periods of increased sediment deposition have been identified. Before the first deforestations ... [more ▼]

In the Ardenne massif, floodplains have recorded fluctuations in the production of sediment and several periods of increased sediment deposition have been identified. Before the first deforestations rivers developed multiple channels in alluvial forests. Floodplains were not well-developed and probably very humid, which explains the presence of peat layers within the alluvial sequences. In the Amblève catchment, the first increased sedimentary deposition of the Holocene occurred during the Bronze Age (3200 BP), in relation to deforestation and the first crop cultures in the area. An organic deposition has occurred around 2700 BP and could correspond with a period of climatic degradation. Several peat layers have also been dated to around 1000 BP and probably indicate very low anthropogenic pressure. From the 11th Century onwards, there was an increase in sedimentation and a greater concentration of charcoal is present in the alluvial deposits. In many catchments there is an important increase in the sedimentation at the end of the 14th century, which can be related to the development of many iron factories. Analyses of slag concentration produced in these former factories allow us to reconstruct the evolution of the floodplain topography since the inception of the iron industries, in relation to the periods of activity. Total sedimentation in the smaller valleys since the initiation of iron industries amounts 0.5 to 1.0 m, which is in most cases about 50% of the total sediment present in the floodplains and corresponds to a mean sedimentation rate ranging between 10 and 20 cm/century. Such values are explained by various former agricultural practices and forest clearings associated with the huge demand for charcoal by the iron industry. About 20 ha of forest were cleared for the yearly consumption of a refining forge or a blast furnace and more than three hundred iron factories existed in the Ardenne Massif between the 14th and the 19th century. [less ▲]

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See detailEvaluation of decision methodologies for analytical method validation
Rozet, Eric ULg; Rudaz, S.; Bouabidi, A. et al

Poster (2009)

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See detailMapping of soil-sediment systems contaminations around a metal-ore smelter : The example of Cu in Lubumbashi (R.D.Congo).
Mpundu, Michel; Mukobo, Robert-Prince; Kaya Muyumba, Donato ULg et al

Poster (2009)

The Katanga is famous for its high richness in metal ores, mainly Cu- and Co-minerals. The ore treatments activities lead to metal spreading in the environment which do endanger the viability of ... [more ▼]

The Katanga is famous for its high richness in metal ores, mainly Cu- and Co-minerals. The ore treatments activities lead to metal spreading in the environment which do endanger the viability of ecosystems and human health. The contaminations of soils and sediments around the Gecamines smelter in Lubumbashi have been evaluated through a multi-scale approach. In the first stage, a reference system about the natural contents in soils has been implemented. The main units of the detailed (1:20 000) soil map have been characterized through field observations and laboratory determinations : acido-basic and organic status, particle size distribution, cationic exchange capacity, total contents in Al, Fe, Mn, Cu, Co, Cd, Pb, Zn. An exploratory mapping of soil contaminations (Cu, Co, Cd, Pb, Zn) centered on the Gecamines cheminey was then conducted as a second stage of the approach. Detailed investigations were finally performed in the Gecamines district where signs of soil degradation were the highest. The spatial continuity and the differenciation (C : C0+C ratio) of the studied parameters appeared weak. No clear effect of the dominant wind direction on the spatial distribution of the contaminants could be identified despite a clear degradation of the ecosystem in the area under dominant wind. However the detailed approach showed evidences of (i) significant wind deposits in the Gecamines district, (ii) and local man-made deposits but also (ii) important processes of redistribution in the landscape through erosion/sedimentation or anthropic activities. Our results point out the difficulty to identify the sources of contaminations in an urban environment and stress the need to have approaches with complementary levels of details. [less ▲]

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See detailSynthesis of Ag/TiO2 hybrid photocatalysts by sol-gel process with new P-alkoxide functionalized ligands
Lambert, Stéphanie ULg; Bied, Catherine; Bodson, Céline et al

Poster (2009)

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See detailContribution to the study of semiochemical slow release formulations. Development of flash chromatographic methods.
Heuskin, Stéphanie ULg; Farmakidis, Julien; Lorge, Stéphanie et al

Poster (2009)

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See detailTransmission des prix dans le secteur laitier en France
Burny, Philippe ULg

Poster (2009)

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See detailWheat root ecophysiology: understanding communication between wheat root system and soil microorganisms.
Delaplace, Pierre ULg; d'Ans, Séverine ULg; Wathelet, Jean-Paul ULg et al

Poster (2009)

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up ... [more ▼]

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up to now, the characterization of such interactions has been mainly focused on root exsudates but volatile organic compounds (VOC) also play a role as chemical messengers in positive interactions occurring in the rhizosphere. We aim to better understand the VOC-mediated chemical ecophysiology of soil. Wheat (Triticum aestivum L.) was chosen in this project based on its importance for food production and industrial applications. [less ▲]

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See detailSet-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailThe effects of purified Z- and E-guggulsterones from guggul-gum extract on human preadipocytes proliferation and differentiation through the study of gene expression of adipose markers.
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailReaching the schools
Nazé, Yaël ULg

Poster (2009)

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See detailProduction and toxicity of γ-decalactone and 4-hydroxydecanoic acid from Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The ... [more ▼]

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The effect of gum tragacanth on the production of γ-decalactone by R. aurantiaca was evaluated. [less ▲]

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See detailHigh-throughput methodology applied to screening of carbohydrate-based surfactant interfacial properties for colloidal system formulations
Razafindralambo, Hary ULg; Blecker, Christophe ULg; Deroanne et al

Poster (2009)

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and ... [more ▼]

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and non-food applications. Based on many functional groups of the carbohydrate part, it is possible to design multiple amphiphilic structures of CBS varying in the hydrophilic head groups (mono-, oligo-, or polysaccharides), hydrophobic tail (mono-, di-, tricatenar) but also in the linker/spacer between them. The main structures of CBS include mono- and bicatenar glycolipids, bolaforms, and gemini. Moreover, CBS compounds can be produced from the most abundant renewable materials allowing large product concept possibilities. Among general properties of surfactants, interfacial properties molecules occupy a fundamental key role for colloidal system formulations since they control most of technological aptitudes required for forming and stabilizing food, cosmetic, agrochemical, detergent, and pharmaceutical products. High-throughput methodology applied to screening of CBS interfacial properties appears crucial for achieving optimum formulations of colloidal systems like foams and emulsions for which small amounts of single or mix surface-active agents are often needed and numerous physical and chemical parameters are involved. This general approach may be applied to any amphiphilic molecules produced from other renewable resources of surface-active compounds like micro-organisms and by direct extraction from vegetable materials. One of the key factors for the development of this methodology is the use of automated instrument systems in laboratory scale which are still lacking in this field compared to those existing in the analytical chemistry and biochemistry areas. [less ▲]

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See detailEvaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts
Grenade, Charlotte ULg; Borget, Pascal; Moniotte, Nicolas et al

Poster (2009)

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The ... [more ▼]

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes. After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF). Materials and methods The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage. Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen. A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture. First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane. For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium. Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm). After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect. Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy. Conclusion Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers. In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers. [less ▲]

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See detailThe strange case of the Of?p stars
Nazé, Yaël ULg; Walborn, N. R.; Martins, F. et al

Poster (2009)

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See detailClinal differentiation during invasion: Senecio inaequidens (Asteraceae) along altitudinal gradients in Europe.
Monty, Arnaud ULg; Mahy, Grégory ULg

Poster (2009)

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic ... [more ▼]

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic change an alien species may undergo during range expansion, e.g. in response to climatic variation with altitude. In the present study, we tested the hypothesis that invasive populations of Senecio inaequidens (Asteraceae) differentiated during migration from two independent introduction sites into divergent altitudinal and climatic zones. We carried out two years of common garden experiments with eight populations from a Belgian altitudinal transect and ten populations from similar French transect. Climatic analysis revealed that the Belgian transect followed a temperature and precipitation gradient. A temperature and summer drought gradient characterized the French study site. We evaluated differentiation and clinal variation in the following characters: days to germination, days to flowering, height at maturity, final plant height and aboveground biomass. Results showed that S. inaequidens populations differentiated in growth traits during invasion. First year of experiment, regressing population growth trait means against source population altitude indicated the presence of clinal variation along both transects. Second year, similar results were found along the French transect, i.e. a reduction in height at maturity, plant height and aboveground biomass with increasing altitude. Including seed mass as a covariate did not change the outcome of the analysis. The possible evolutionary causes for the differentiation observed are discussed. [less ▲]

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See detailInvasive plant species management tests in Southern Belgium
Delbart, Emmanuel ULg; Halford, Mathieu ULg; Pieret, Nora et al

Poster (2009)

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See detailMCF-7/BOS cells membrane proteome: comparison of two isolation methods using mass spectrometry
Bertrand, Virginie ULg; Massart, Anne-Cécile ULg; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2009)

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low ... [more ▼]

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low abundance. These membrane markers have to be accessible to antibodies and should be potential therapeutic targets. The tests were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain a pure membrane fraction to facilitate the analysis of the sample. To isolate transmembrane proteins, we compared two methods. The first one used different extraction cycles characterized by different buffers to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. [less ▲]

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See detailGenetic structure of Barn swallow (Hirundo rustica).
Dardenne, Sophie ULg; Stevens, Virginie ULg; Hollander, F. et al

Poster (2009)

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See detailEvaluation of the endorcine disrupting chemicals contamination in the North Sea porpoise population using in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Van Der Heiden, Edwige ULg et al

Poster (2009)

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental ... [more ▼]

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental quality and posing ecological risks. Marine mammals inhabiting polluted environments accumulate high levels of these chemicals, so they can be considered good indicators of marine pollution. Thirteen major organochloride pollutants were chosen to make subject of this study: o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. All these chemicals will have their endocrine disrupting effects characterized individually and collectively by report gene expression assays. The MCF7-ERE cells used in these assays were produced in the Molecular Biology and Genetic Engineering Laboratory of the University of Liège and are originated from a human mammary carcinoma. They carry a gene expressing the synthesis of luciferase and controlled by oestrogen receptors. Then, the thirteen chemicals will be searched in the blubber tissues of porpoises by the use of gas chromatography-mass spectrometry. The blubber samples will also have their endocrine disrupting effect characterized. For the moment, the estrogenic effects of the HCH isomers were put in evidence. The next steps of this work must be soon accomplished. It is expected a great level of these compounds to be found in the samples. On the other hand, their endocrine disrupting effects and how they act in agonist, antagonist and synergist ways are subject that still needs to be cleared. [less ▲]

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See detailThe fast regulation of aromatase activity by phosphorylations is species and tissue-independent.
Charlier, Thierry ULg; Harada, Nobuhiro; Ball, Gregory F. et al

Poster (2009)

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See detailOutreach goals of the Europa Jupiter System Mission
Blanc, M.; Coustenis; Nazé, Yaël ULg et al

Poster (2009)

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See detailCarbon cycling in the mixolimnion of Lake Kivu (East Africa)
Borges, Alberto ULg; Descy, J.-P.; Delille, Bruno ULg et al

Poster (2009)

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

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See detailAchromatization of solar concentrator using diffractive optics
Languy, Fabian ULg; Lenaerts, Cedric ULg; Loicq, Jerôme ULg et al

Poster (2009)

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux ... [more ▼]

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux more uniform. In addition lenses are less prone to errors of manufacture and can be easily duplicated. Unfortunately, optical materials are very dependent on the wavelength which causes important chromatic aberrations. One can think about achromatic doublets but doublets are thick, heavy and require exotic glasses which make them expensive. During the conference, I would like to present an innovative way to achromatize solar concentrators using hybrid (refractive/diffractive) lenses. Indeed, an hybrid lens is as thin as a Fresnel lens and is made in only one glass. But diffractive lenses suffer from limited broadband diffraction efficiency due to spurious orders. To overcome the lake of efficiency we are investigating the use of multi-layer diffractive optical element. This technique allows to achieve an efficiency greater than 97% over the full visible spectrum using only three components (e.g. two optical elements + air). [less ▲]

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