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See detailTransmission des prix dans le secteur laitier en France
Burny, Philippe ULg

Poster (2009)

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See detailWheat root ecophysiology: understanding communication between wheat root system and soil microorganisms.
Delaplace, Pierre ULg; d'Ans, Séverine ULg; Wathelet, Jean-Paul ULg et al

Poster (2009)

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up ... [more ▼]

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up to now, the characterization of such interactions has been mainly focused on root exsudates but volatile organic compounds (VOC) also play a role as chemical messengers in positive interactions occurring in the rhizosphere. We aim to better understand the VOC-mediated chemical ecophysiology of soil. Wheat (Triticum aestivum L.) was chosen in this project based on its importance for food production and industrial applications. [less ▲]

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See detailSet-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailThe effects of purified Z- and E-guggulsterones from guggul-gum extract on human preadipocytes proliferation and differentiation through the study of gene expression of adipose markers.
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailProduction and toxicity of γ-decalactone and 4-hydroxydecanoic acid from Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The ... [more ▼]

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The effect of gum tragacanth on the production of γ-decalactone by R. aurantiaca was evaluated. [less ▲]

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See detailContribution to the study of semiochemical slow release formulations. Development of flash chromatographic methods.
Heuskin, Stéphanie ULg; Farmakidis, Julien; Lorge, Stéphanie et al

Poster (2009)

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See detailEvaluation of the endorcine disrupting chemicals contamination in the North Sea porpoise population using in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Van Der Heiden, Edwige ULg et al

Poster (2009)

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental ... [more ▼]

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental quality and posing ecological risks. Marine mammals inhabiting polluted environments accumulate high levels of these chemicals, so they can be considered good indicators of marine pollution. Thirteen major organochloride pollutants were chosen to make subject of this study: o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. All these chemicals will have their endocrine disrupting effects characterized individually and collectively by report gene expression assays. The MCF7-ERE cells used in these assays were produced in the Molecular Biology and Genetic Engineering Laboratory of the University of Liège and are originated from a human mammary carcinoma. They carry a gene expressing the synthesis of luciferase and controlled by oestrogen receptors. Then, the thirteen chemicals will be searched in the blubber tissues of porpoises by the use of gas chromatography-mass spectrometry. The blubber samples will also have their endocrine disrupting effect characterized. For the moment, the estrogenic effects of the HCH isomers were put in evidence. The next steps of this work must be soon accomplished. It is expected a great level of these compounds to be found in the samples. On the other hand, their endocrine disrupting effects and how they act in agonist, antagonist and synergist ways are subject that still needs to be cleared. [less ▲]

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See detailHigh-throughput methodology applied to screening of carbohydrate-based surfactant interfacial properties for colloidal system formulations
Razafindralambo, Hary ULg; Blecker, Christophe ULg; Deroanne et al

Poster (2009)

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and ... [more ▼]

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and non-food applications. Based on many functional groups of the carbohydrate part, it is possible to design multiple amphiphilic structures of CBS varying in the hydrophilic head groups (mono-, oligo-, or polysaccharides), hydrophobic tail (mono-, di-, tricatenar) but also in the linker/spacer between them. The main structures of CBS include mono- and bicatenar glycolipids, bolaforms, and gemini. Moreover, CBS compounds can be produced from the most abundant renewable materials allowing large product concept possibilities. Among general properties of surfactants, interfacial properties molecules occupy a fundamental key role for colloidal system formulations since they control most of technological aptitudes required for forming and stabilizing food, cosmetic, agrochemical, detergent, and pharmaceutical products. High-throughput methodology applied to screening of CBS interfacial properties appears crucial for achieving optimum formulations of colloidal systems like foams and emulsions for which small amounts of single or mix surface-active agents are often needed and numerous physical and chemical parameters are involved. This general approach may be applied to any amphiphilic molecules produced from other renewable resources of surface-active compounds like micro-organisms and by direct extraction from vegetable materials. One of the key factors for the development of this methodology is the use of automated instrument systems in laboratory scale which are still lacking in this field compared to those existing in the analytical chemistry and biochemistry areas. [less ▲]

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See detailThe strange case of the Of?p stars
Nazé, Yaël ULg; Walborn, N. R.; Martins, F. et al

Poster (2009)

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See detailEvaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts
Grenade, Charlotte ULg; Borget, Pascal; Moniotte, Nicolas et al

Poster (2009)

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The ... [more ▼]

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes. After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF). Materials and methods The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage. Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen. A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture. First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane. For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium. Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm). After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect. Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy. Conclusion Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers. In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers. [less ▲]

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See detailClinal differentiation during invasion: Senecio inaequidens (Asteraceae) along altitudinal gradients in Europe.
Monty, Arnaud ULg; Mahy, Grégory ULg

Poster (2009)

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic ... [more ▼]

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic change an alien species may undergo during range expansion, e.g. in response to climatic variation with altitude. In the present study, we tested the hypothesis that invasive populations of Senecio inaequidens (Asteraceae) differentiated during migration from two independent introduction sites into divergent altitudinal and climatic zones. We carried out two years of common garden experiments with eight populations from a Belgian altitudinal transect and ten populations from similar French transect. Climatic analysis revealed that the Belgian transect followed a temperature and precipitation gradient. A temperature and summer drought gradient characterized the French study site. We evaluated differentiation and clinal variation in the following characters: days to germination, days to flowering, height at maturity, final plant height and aboveground biomass. Results showed that S. inaequidens populations differentiated in growth traits during invasion. First year of experiment, regressing population growth trait means against source population altitude indicated the presence of clinal variation along both transects. Second year, similar results were found along the French transect, i.e. a reduction in height at maturity, plant height and aboveground biomass with increasing altitude. Including seed mass as a covariate did not change the outcome of the analysis. The possible evolutionary causes for the differentiation observed are discussed. [less ▲]

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See detailInvasive plant species management tests in Southern Belgium
Delbart, Emmanuel ULg; Halford, Mathieu ULg; Pieret, Nora et al

Poster (2009)

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See detailGlass production in late antiquity
Van Wersch, Line ULg; Mathis, François ULg; Dupuis, Thomas ULg et al

Poster (2009)

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See detailGenetic structure of Barn swallow (Hirundo rustica).
Dardenne, Sophie ULg; Stevens, Virginie ULg; Hollander, F. et al

Poster (2009)

Detailed reference viewed: 12 (3 ULg)
See detailThe fast regulation of aromatase activity by phosphorylations is species and tissue-independent.
Charlier, Thierry ULg; Harada, Nobuhiro; Ball, Gregory F. et al

Poster (2009)

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See detailCarbon cycling in the mixolimnion of Lake Kivu (East Africa)
Borges, Alberto ULg; Descy, J.-P.; Delille, Bruno ULg et al

Poster (2009)

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

Detailed reference viewed: 47 (19 ULg)
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See detailAchromatization of solar concentrator using diffractive optics
Languy, Fabian ULg; Lenaerts, Cedric ULg; Loicq, Jerôme ULg et al

Poster (2009)

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux ... [more ▼]

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux more uniform. In addition lenses are less prone to errors of manufacture and can be easily duplicated. Unfortunately, optical materials are very dependent on the wavelength which causes important chromatic aberrations. One can think about achromatic doublets but doublets are thick, heavy and require exotic glasses which make them expensive. During the conference, I would like to present an innovative way to achromatize solar concentrators using hybrid (refractive/diffractive) lenses. Indeed, an hybrid lens is as thin as a Fresnel lens and is made in only one glass. But diffractive lenses suffer from limited broadband diffraction efficiency due to spurious orders. To overcome the lake of efficiency we are investigating the use of multi-layer diffractive optical element. This technique allows to achieve an efficiency greater than 97% over the full visible spectrum using only three components (e.g. two optical elements + air). [less ▲]

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See detailSeasonal variability of carbon dioxide and methane in the rivers and lagoons of Ivory Coast (West Africa)
Koné, Y. J. M.; Abril, G.; Delille, Bruno ULg et al

Poster (2009)

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See detailTransinusoidal portal vein embolisation with Onyx: feasibility study in pigs
Smits; Hav; Vanlengenhoeve et al

Poster (2009)

Detailed reference viewed: 37 (1 ULg)
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See detailCarbon cycling in the mixolimnion of Lake Kivu (East Africa)
Borges, Alberto ULg; Descy, J.-P.; Delille, Bruno ULg et al

Poster (2009)

Detailed reference viewed: 25 (3 ULg)
See detailThe Europlanet national nodes network
Fouchet, T.; Heward, A.; Chatzchristou, E. T. et al

Poster (2009)

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See detailSynthèse et propriétés tensioactives de nouveaux surfactants provenant de matières premières renouvelables.
Laurent, Pascal ULg; Razafindralambo, Hary ULg; Wathelet, Bernard ULg et al

Poster (2009)

A l'heure actuelle, le bioraffinage se présente de plus en plus comme une alternative prometteuse aux filières pétrochimiques puisqu'il vise à la fois le remplacement d'une partie du pétrole comme source ... [more ▼]

A l'heure actuelle, le bioraffinage se présente de plus en plus comme une alternative prometteuse aux filières pétrochimiques puisqu'il vise à la fois le remplacement d'une partie du pétrole comme source d'énergie et le développement de produits chimiques issus de la biomasse (végétale principalement) tels que détergents, produits phytopharmaceutiques, dissolvants, matières plastiques, etc. La valorisation de carbohydrates provenant de matières premières renouvelables fait ainsi actuellement l'objet de nombreuses recherches. Dans ce cadre, la synthèse de nouveaux tensioactifs non ioniques dérivés des acides Dglucuronique et D-galacturonique a été effectuée. [less ▲]

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See detailEvaluation of the antitumor activity of 16K prolactin
Kinet, Virginie; Nguyen, Ngoc-Quynh-Nhu ULg; Sabatel, Céline et al

Poster (2009)

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See detailReptile cell cultures for nucleolar studies
Lamaye, Françoise; Thiry, Marc ULg

Poster (2009)

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See detailMCF-7/BOS cells membrane proteome: comparison of two isolation methods using mass spectrometry
Bertrand, Virginie ULg; Massart, Anne-Cécile ULg; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2009)

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low ... [more ▼]

Membrane proteins play an important role in biological processes but their isolation and quantification using classical techniques is often limited due to their poor solubility and relatively low abundance. These membrane markers have to be accessible to antibodies and should be potential therapeutic targets. The tests were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain a pure membrane fraction to facilitate the analysis of the sample. To isolate transmembrane proteins, we compared two methods. The first one used different extraction cycles characterized by different buffers to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. [less ▲]

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See detailNon destructive testing by digital shearography using a Savart plate
Michel, Fabrice ULg; Blain, Pascal ULg; Renotte, Yvon ULg et al

Poster (2009)

Shearography is a growing optical technique in the field of non-destructive testing (NDT). Hololab developed an out of plane, in line and almost common path interferometer based on polarization states ... [more ▼]

Shearography is a growing optical technique in the field of non-destructive testing (NDT). Hololab developed an out of plane, in line and almost common path interferometer based on polarization states separation using a coated prism for digital phase-shifting shearography. This setup is efficient but does not allow varying the shearing direction, that is an important parameter for defects detection and quantification. To overcome this disadvantage, the coated prism is substituted by a Savart plate device that allows scanning several shearing directions by rotating the device around the light propagation axis. Many advantages of the prism substitution are discussed. The behaviour of the Savart plate as a shearing device is experimentally analyzed to optimize its integration within the interferometer. Recorded phasemaps in NDT for different shearing directions are presented. [less ▲]

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See detailThe use of Macronet resins to recover γ-decalactone produced by Rhodotorula aurantiaca from the culture broth
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to recover the γ-decalactone produced by R. aurantiaca from the culture broth using three Macronet resins (MN-100, MN-102 and MN-202).

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See detailReintroduction & effects on their environment of indigenous plants of the Cerrado
Buisson, Elise; Le Stradic, Soizig ULg; Negreiros, Daniel et al

Poster (2009)

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See detailDevelopment of a financial model for Geriatric Day Hospitals
Gillain, Daniel ULg; Fecher-Bourgeois, Fabienne ULg; Velghe, A. et al

Poster (2009)

Detailed reference viewed: 31 (19 ULg)
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See detailThe post-harvest quality of bananas is determined by pre-harvest factors
de Lapeyre de Bellaire, Luc; Chillet, Marc; Lassois, Ludivine ULg et al

Poster (2009)

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See detailDéveloppement des filières Jatropha décentralisées au Sénégal
Terren, Marieke ULg; Saverys, S.; Jacquet de Haverskercke, P et al

Poster (2009)

Detailed reference viewed: 48 (11 ULg)
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See detailHolocene environmental changes in lake sediments from Northern Chilean Patagonia (45-48°S)
Nuttin, L.; Fagel, Nathalie ULg; Bertrand, S. et al

Poster (2009)

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See detail3D anatomy of Heinrich Layer 2.
Van Rooij, D.; Zaazi, N.; Fagel, Nathalie ULg et al

Poster (2009)

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See detailENVIRONMENTAL ASPECTS OF CLAY EXPLOITATION FOR FIRED CLAY BRICKS IN CAMEROON
Njoya, A.; Bastin, D.; Melo, U. et al

Poster (2009)

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See detailNutritional markers course after oral supplementation with different forms of iodine in Holstein non-lactating cows
Guyot, Hugues ULg; VanParijs, Sandrine; Uyttenhoef, Aude et al

Poster (2009)

Iodine (I) deficiency is commonly reported in cattle around the world and is often associated with clinical or sub-clinical diseases. As most of clinical signs are not pathognomonic, diagnosis has to be ... [more ▼]

Iodine (I) deficiency is commonly reported in cattle around the world and is often associated with clinical or sub-clinical diseases. As most of clinical signs are not pathognomonic, diagnosis has to be confirmed by biochemical analyses such as plasmatic inorganic iodine (PII) or urinary I. Different oral mineral forms of I are available in Europe for cattle. The aim of the study was to compare the kinetic of I in blood and urine in non-lactating cows, following oral administration of different forms of I. Five groups of 6 non-lactating cows (aged 6 ± 2 years, weight 604 ± 89 kg), receiving the same ration (11 kg dry matter) and housed in the same conditions (tied-stall and straw) underwent a double-blinded trial during 2 months. Excepting in Group A (Control), all cows received a daily oral supplementation of I equal to 5 ppm, in the form of Ca(IO3)2 (Group B), KI (Group C), organic form of I 1 (Group D) and organic form of I 2 (Group E). Formulas of the organic forms of I are not public and coverable by patent. Supplementation was stopped at T45. Blood and urine samples were taken at T0, T15, T30 and T60. Thyroxine (T4) was measured at T0, T30 and T60 while PII and urinary I were measured at the 4 times of the trial. Student-t test and multiple comparisons of means (mix crossed model) were used to compare I and T4 concentrations between groups and times. All characteristics about the cows and I levels in blood or urine were not significantly different at T0 (p>0.1). There was no significant difference (p>0.1) between groups B, C, D, E at the different times of the trial. PII and urinary I in Group A were significantly lower than in other groups (p<0.01) at T15 and T30. Highest concentrations of I (PII up to 242 ± 30 µg/L and urinary I up to 2326 ± 439 µg/L) were reached at T15 for groups B, D and E. At T60, PII (19 ± 4 µg/L) and urinary I (110 ± 29 µg/L) of all groups reached the basal level. A good correlation was found between PII and urinary I (r² = 0.77). No significant differences were found about T4 (67 ± 10 nmol/L) in all groups and times (p>0.1). PII and urinary I are good markers to assess I nutritional status. No difference was found between either inorganic or organic forms of I, nor between them. Concerning the mineral forms of I, Ca(IO3)2 might be preferably used because of its higher stability in the mineral complexes. [less ▲]

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See detailPercutaneous penetration studies of ibuprofenencapsulated into nanoparticles using different skin membrane models
Destrée, Caroline; Wouters, Johan; Deleu, Magali ULg et al

Poster (2009)

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See detailCHARACTERIZATION AND OPTIMIZATION OF PEPTIDE ENCAPSULATION IN PEGYLATED LIPOSOMES
Ducat, Emilie ULg; Brion, Michael; Evrard, Brigitte ULg et al

Poster (2009)

Purpose: Print 3G is a peptidic antagonist of oncoprotein involved in breast cancer, containing 25 amino acids. The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two ... [more ▼]

Purpose: Print 3G is a peptidic antagonist of oncoprotein involved in breast cancer, containing 25 amino acids. The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two formulations composed of SPC:CHOL:mPEG-750-DSPE (47:47:6) or SPC:CHOL:mPEG-2000-DSPE (47:47:6) were investigated. Methods: Unilamellar vesicles containing either mPEG750 or mPEG2000 were prepared by hydration of lipid films method. Unfortunately, a loss of Print 3G was observed during the different steps of this manufacturing technique giving rise to encapsulation efficiencies close to 0 %. Thus, the freeze-thawing method was used to enhance the amount of Print 3G encapsulated into blank liposomes prepared using the above procedure. Because many factors may influence the peptide entrapment into the vesicles (number of freeze-thawing cycles, lipid concentration, peptide concentration, mixing time and liposome composition), a design of experiment was performed (for the screening, a Plackett and Burman plan; for the optimization, a central composite design). Results: The encapsulation efficiencies obtained by the freeze-thawing method in standard conditions, varied between 17.26 ± 0.46 % (n=3) for liposomes containing mPEG750 and 26.20 ± 7.98 % (n=3) for those comprising mPEG2000. Among the different considered factors, the screening permitted to identify two factors having a positive and significant influence on the encapsulation efficiencies: the number of freeze-thawing cycles and the lipid concentration, while the presence of mPEG2000 or mPEG750 had a relatively weak effect on the encapsulation. Concerning the peptide concentration and the mixing time, no influence was revealed. For the second part of the DOE, the positive factors were optimized for the liposomes containing mPEG2000. The obtained results for liposomes containing mPEG2000 revealed a theoretical optimum at 64.75 ± 3.55 % when 11 freeze-thawing cycles were applied and for the following lipid concentrations: 36.1 mM SPC, 36.1 mM CHOL and 4mM mPEG-2000-DSPE. The experimental results showed an encapsulation efficiency of 62.68 ± 2.93 %. Conclusion: Changing the manufacturing technique permitted a significant encapsulation of Print 3G into liposomes. The DOE led to a significant improvement of encapsulation efficiencies for the liposomes containing mPEG2000. Thereafter, an optimization design for liposomes containing mPEG750 will be started. Acknowledgements: This work was supported by the Ministry of the Walloon Region. [less ▲]

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See detailCHARACTERIZATION AND OPTIMIZATION OF PEPTIDE ENCAPSULATION IN PEGYLATED LIPOSOMES
Ducat, Emilie ULg; Brion, Michael; Evrard, Brigitte ULg et al

Poster (2009)

Purpose: The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two formulations (SPC:CHOL:mPEG-750-DSPE (47:47:6) or SPC:CHOL:mPEG-2000-DSPE (47:47:6)) were investigated ... [more ▼]

Purpose: The purpose of this work is to study the peptide encapsulation into PEGylated liposomes. Two formulations (SPC:CHOL:mPEG-750-DSPE (47:47:6) or SPC:CHOL:mPEG-2000-DSPE (47:47:6)) were investigated. Methods: Blank SUV liposomes were prepared by the lipid film hydration and the encapsulation was achieved by applying freeze-thawing cycles. Because many factors may influence peptide entrapment (number of freeze-thawing cycles (NC), lipid concentration (LC), peptide concentration (PC), mixing time (MT) and liposome composition (COMP)), a design of experiment (DOE) was performed. Results: The screening permitted to identify two factors having a positive and significant influence on the encapsulation efficiencies (NC and LC) while the liposome composition had a relatively weak effect. For the second part of the DOE, the positive factors were optimized for liposomes containing mPEG2000. The obtained results revealed a theoretical optimum at 64.75±3.55% when 11 cycles were applied and for the following LC: 36.1mM SPC, 36.1mM CHOL and 4mM mPEG-2000-DSPE. Experimental results showed an encapsulation efficiency of 62.68±2.93%. Conclusion: The DOE led to significant improvement of encapsulation for liposomes containing mPEG2000. Thereafter, an optimization design for liposomes containing mPEG750 will be started. Acknowledgements: This work was supported by the Ministry of the Walloon Region. [less ▲]

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