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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Alvarez Gonzalez, M-L et al

Poster (2007)

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See detailAnalytical determination of Isomaltooligosaccharides mixture known as emerging prebiotics.
Goffin, Dorothée ULg

Poster (2007)

Isomaltooligosaccharides (IMOs) are non-digestible oligosaccharides, considered as prebiotics and therefore aim to selectively feed probiotics indigenous to the human colon. Recent data obtained in human ... [more ▼]

Isomaltooligosaccharides (IMOs) are non-digestible oligosaccharides, considered as prebiotics and therefore aim to selectively feed probiotics indigenous to the human colon. Recent data obtained in human subjects, support the involvement of dietary oligosaccharides in physiological processes in the different intestinal cell type and also outside the gastrointestinal tract (e.g. hormone production, lipid and carbohydrate metabolism). IMOs consists of glucose monomers linked by at least one α-1-6, or in a lower proportion α -1-3 (nigerose family) or α -1-2 (kojibiose family) glucosidic linkages. In our case they are produced enzymatically from corn starch. It results in a very complex mixture with molecules characterized at the same time by their DP value (from 2 to ~20), linkages types (α-1-2, 3 or 6) and the proportion and position of each type of linkage (only α -1-6 or combined types). The challenge of this study was to find a qualitative and quantitative method to characterize the syrups. In a subsequent study, every unidentified peak could be determined by NMR or Mass spectrometry. Three different chromatographic methods have been tested and compared over their selectivity, sensibility, robustness, applicability and their quantitative power. [less ▲]

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See detailPlant-based production of human lysozyme mutants
Tocquin, Pierre ULg; Dumoulin, Mireille ULg; Dony, Nicolas ULg et al

Poster (2007)

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See detailHorizontal Evaluation Method for the Implementation of the Construction Products Directive (HEMICPD)
Lor, Marc; Vause, Kevin; Dinne, Karla et al

Poster (2007)

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See detailSemi-separative isolation of Fn-type inulin from hydrolised globe artichoke inulin.
Ronkart, Sébastien; Blecker, Christophe ULg; Fourmanoir, Hélène et al

Poster (2007)

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See detailThe Aznalcóllar disaster: An in-depth PIXE study of the pirite mine spill of 1998
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Álvarez, M. A. et al

Poster (2007)

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See detailFatty acids and stable isotopes in fish and harbour porpoises (Phocoena phocoena) from the North Sea: further insights in their trophic relationships
Drouguet, Olivier; Caut, Stéphane; Haelters, Jan et al

Poster (2007)

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises ... [more ▼]

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises displaying lesions linked to fishing nets has also increased this last decade. The increased abundance of porpoises together with the increase in numbers of bycaught animals raised the question of possible competition between porpoises and fishermen in the North Sea. Therefore, a detailed view on harbour porpoise trophic ecology in this region is crucial for their conservation. Stomachs of the washed ashore porpoises were often empty and provided limited information on their recent diet. Another way of looking into the diet of marine mammals is the analysis of blubber. Stable isotopes ratio (d13C and d15N, SI) and fatty acid composition (20 fatty acids, FA) were analyzed in muscle and blubber of 10 freshly stranded harbour porpoises and in 60 potential prey fish from 10 species collected in the North Sea. The SI ratio and FA composition of these fish enabled a clear classification in different trophic levels and different trophic niches. The FA composition was very similar between harbour porpoise blubber and demersal fish, underlining a preferential predation on that resource for the porpoises investigated. A bias might exist for the porpoises: most of the animals were juveniles, and washed ashore during spring. Porpoises might display other feeding habits in other life stages, other periods of the year. These preliminary results however indicate the enhanced accuracy of crossing these biomarker approaches. [less ▲]

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See detailCarbon dioxide dynamics in the tropical Ebrié lagoon (Ivory Coast)
Koné, Y. J. M.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007)

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See detailPhotodynamic therapy for cutaneous carcinoma
NIKKELS, Arjen ULg; thirion, L.; QUATRESOOZ, Pascale ULg et al

Poster (2007)

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See detailLast two Millennia atmospheric lead and heavy metals inputs in a Belgian peat bog: regional to global Human impacts
De Vleeschouwer; Gerard; Goormaghtigh et al

Poster (2007)

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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailCannibalism in Anchovy (Engraulis encrasicolus) in the North Aegean Sea (Greece)
Gennotte, Vincent ULg; Torre, Michele; Kallianiotis, Argyris

Poster (2007)

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 ... [more ▼]

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 % larvae. Egg consumption was observed during the night and larval consumption during the day. Cannibalism seems to be an opportunistic feeding strategy of anchovy, depending on prey availability and proximity of adults, larvae and eggs in relation with the diel vertical migrations of the fish. [less ▲]

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See detailVeszelyite: A New Blue-Green Maya Pigment
Garcia Moreno, Renata; Mathis, François ULg; Strivay, David ULg

Poster (2007)

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See detailControlled release of drug from multicomponent device
Nizet, Dominique; Zalfen, Alina; Jérôme, Christine ULg et al

Poster (2007)

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See detailLa jonction thalweg-cours d'eau : pour une gestion dès l'amont
Dautrebande, Sylvia; Degre, Aurore ULg; Dagnelies, Joelle

Poster (2007)

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See detailMechanical stress strongly induces interleukin-6 production by osteoblasts: a new in vitro 3D compression model
Sanchez, Christelle ULg; Gabay, Odile; Salvat, Colette et al

Poster (2007)

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See detailMechanical stress strongly induces interleukin-6 production by osteoblasts: a new in vitro 3D compression model
Sanchez, Christelle ULg; Gabay, Odile; Salvat, Colette et al

Poster (2007)

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See detailProvenance of Belgian Merovingian garnets by PIXE on IPNAS Cyclotron
Mathis, François ULg; Vrielynck, Olivier; Laclavetine, Kilian et al

Poster (2007)

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See detailAnalysis of furan by GC/MS
Scholl, Georges ULg; Eppe, Gauthier ULg; Scippo, Marie-Louise ULg et al

Poster (2007)

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See detailFibrous mucosa preservation with NobelGuide
Bolland, Fabrice; Van Heusden, Alain ULg

Poster (2007)

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See detailDirecting the lipopeptide Mycosubtilin biosynthesis toward C17:0 branched isoform influences the expression of cspB and cspC in Bacillus subtilis
Guez, Jean-Sébastien; Drucbert, A.; Müller, C. et al

Poster (2007)

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See detailEnrichment of anhydrous milk fat in polyunsatured fatty acid residues from linseed and rapeseed oil through enzymatic interesterification
Aguedo, Mario ULg; Hanon, Emilien ULg; Danthine, Sabine ULg et al

Poster (2007)

The interesterification, or ester exchange, between two fats leads to the rearrangement of acyl moieties in both. The use of a sn-1,3-specific lipase confines the exchange of fatty acid residues to the sn ... [more ▼]

The interesterification, or ester exchange, between two fats leads to the rearrangement of acyl moieties in both. The use of a sn-1,3-specific lipase confines the exchange of fatty acid residues to the sn-1 and sn-3 positions of triacylglycerides (TAG), generating products with characteristics that cannot be obtained through a chemical process or a blending. Such reactions require mild conditions with no solvent needed and they yield no unhealthful trans fatty acids, justifying the stepped-up interest of enzymatic interesterification for the production of margarines and other food fats. The aim of this work was to use enzymatic interesterification to enrich anhydrous milk fat (AMF) with unsaturated fatty acid C18 residues from linseed oil (LO) and eventually from rapeseed oil (RO) through some binary blends and one ternary blend. For that, the 1,3-specific lipase from Thermomyces lanuginosa (Lipozyme TL IM) was used in solvent-free batch and micro-aqueous reactions and fat blends with different mass ratios were tested. The evolution of TAG profiles, of interesterification degre (ID) and of free fatty acids (FFA), was followed along the reactions. Determination of dropping points (DP) and solid fat contents (SFC) enabled a rheological characterization of the products. The end products were also characterized for their oxidative stability and their textural properties. [less ▲]

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See detailRandom Subwindows and Multiple Output Decision Trees for Generic Image Annotation
Dumont, Marie; Marée, Raphaël ULg; Geurts, Pierre ULg et al

Poster (2007)

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See detailSYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION
Schneider, Nicole ULg; Mouithys-Mickalad, Ange ULg; Lejeune, Jean-Philippe ULg et al

Poster (2007)

Introduction : An oxidant activity has been implicated in the onset of equine osteoarthritis. Most of the studies have been done on articular chondrocytes, but little is known about the role of ... [more ▼]

Introduction : An oxidant activity has been implicated in the onset of equine osteoarthritis. Most of the studies have been done on articular chondrocytes, but little is known about the role of synoviocytes. Objective : Our aim was to investigate if equine articular chondrocytes, primary synoviocytes or synoviocytes of a continuous cell line are able to produce free radicals after exposure to anoxia and re-oxygenation. Methods : By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10exp7 cells; O2 consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n = 3 for each cell line). Results : Both types of synoviocytes showed a high O2 consumption, which was slower after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O2 consumption, unchanged by anoxia, and no free radical production. Conclusion : These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis. [less ▲]

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See detailSpring CO2 dynamics within sea ice: abiotical versus biological control.
Delille, Bruno ULg; Schoemann, Véronique; Becquevort, Sylvie et al

Poster (2007)

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See detailNumerical modelling of coupled mechanics and gas transfer
Gerard, Pierre ULg; Charlier, Robert ULg; Barnichon, Jean-Dominique et al

Poster (2007)

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See detailControlled release of drugs from an original multi-component device
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailModelling spray drift using a modified air pollution Gaussian tilting plume model
Stainier, Charles; Destain, Marie-France ULg; Schiffers, Bruno ULg et al

Poster (2007)

The objective of this research is to evaluate a Gaussian tilting plume model that takes into account the spray characteristics of agricultural nozzles to predict drift. The application of this type of ... [more ▼]

The objective of this research is to evaluate a Gaussian tilting plume model that takes into account the spray characteristics of agricultural nozzles to predict drift. The application of this type of model has proved to be effective for aerial pollution applications. To be applied successfully to the spray drift, the model has to give accurate predictions of the deposits regarding the spray and material characteristics as well as the weather parameters. To reach this objective, the model parameters must be correctly set based on appropriate theoretical basis and experimental data. Based on wind tunnel measurements, the effect of the most important characteristics of spray droplets from an agricultural nozzle has been modelled using a Gaussian tilting plume approach by discretizing the different droplet classes. Although the theoretical basis of the model is simple, the predicted drift appeared to be in relatively good agreement with the experimental results. The discrepancies could be explained by poor fitting of the dispersion model parameters. Further work is required to optimise the value of the model parameters. [less ▲]

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See detailSugar beet leaves as source of lyase in bioprocess producing green note aldehydes
Rabetafika, Holy-Nadia ULg; Gigot, Cédric ULg; Fauconnier, Marie-Laure ULg et al

Poster (2007)

Sugar beet leaves were used as source of lyase in a biocatalytic process for the production of C6-aldehydes. Their hydroperoxide lyase activity was revealed important. The optimum values of pH and ... [more ▼]

Sugar beet leaves were used as source of lyase in a biocatalytic process for the production of C6-aldehydes. Their hydroperoxide lyase activity was revealed important. The optimum values of pH and temperature for reactions were respectively 6.7 and 22 °C. A simple and fast process providing high molar conversion of hydroperoxides to aldehydes is proposed. The yield of the main product cis-3-hexenal reached 80% after only 2 min and was stabilised by acidifying the reaction medium to pH 2. [less ▲]

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See detailComposition of degumming residues from oil physical refining : valorization for food application
Pierart, Céline ULg; Cavillot, Véronique; Kervyn de Meerendré, M. et al

Poster (2007)

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See detailSound production mechanism in the clownfish Amphiprion clarkii (Amphiprioninae, Pomacentridae)
Colleye, Orphal ULg; Herrel, Anthony; Mauguit, Quentin et al

Poster (2007)

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic ... [more ▼]

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic interactions. Although clownfish sounds were recorded since 1930, the mechanism of sound production has remained unresolved. The sounds used to describe the sonic mechanism were directed towards hetero- and conspecifics that approach their sea anemone host. Sound recordings were synchronized using a high speed video (500 fps) coupled or not with an X-ray system. These systems allowed to quantify the movements of external and internal bones during sound production. Sounds were typically accompanied by rapid (< 30 ms) head movements such as elevation of the skull, lowering of the hyoid bar and the anterior part of the branchial basket, retraction of the pectoral girdle, and finally closing of the mouth. Synchronization of sound pulses with X-ray images indicates that sound is produced when the hyoid apparatus is completely lowered and the mouth closed by a previously unknown mechanism. Dissections of freshly dead specimens reveal an unusual ligament responsible for the rapid mouth closing. This ligament joins the hyoid bar to the internal part of the mandible. Acting as a cord, it forces the mandible to turn around its articulation during the lowering of the anterior part of the branchial basket, forcing the mouth to close. Sounds result from the collision of the jaw teeth, transferring energy to the jaws that are presumably the sound radiator. [less ▲]

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See detailEffect of cyclodextrin derivatives on bronchial epithelial cell layer permeability
Belhadj-Salem, Leila; Bosquillon, Cynthia; Delattre, Luc ULg et al

Poster (2007)

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See detailHigh resolution 16 row computed tomography examination of the canine thorax
De Busscher; Bolen, Géraldine ULg; Cavrenne et al

Poster (2007)

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See detailPotential of Shallow Lake Systems to Trace Environmental Changes Caused by Earthquakes
Avsar; Boes; Hubert-Ferrari et al

Poster (2007)

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See detailControlled release of drugs from an original multi-component device
Nizet, dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailThe XMM-LSS Survey : properties and two-point angular correlations of point-like sources
Garcet, O.; Gandhi, P.; Disseau, L. et al

Poster (2007)

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See detailAlien invasive species and climate change: overview of research activities
Vanderhoeven, Sonia ULg; Saad, Layla ULg; Tiébré, Marie-Solange et al

Poster (2007)

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See detailGlobal morphology of substorm growth phases observed by the IMAGE-SI12 imager
Blockx, Caroline ULg; Gérard, Jean-Claude ULg; Coumans, Valérie ULg et al

Poster (2007)

Growth phases are observed to start from less than 30 minutes to over an hour before the substorm onset. The sector of maximum proton precipitation during the growth phase is generally located around 2200 ... [more ▼]

Growth phases are observed to start from less than 30 minutes to over an hour before the substorm onset. The sector of maximum proton precipitation during the growth phase is generally located around 2200 MLT. It rapidly moves in local time by about 1.2 hour toward midnight at the time of the onset. The open magnetic flux increases by as much as a 33% during the growth phase. The mean value of the open flux at the end of the growth phase, immediately preceding the substorm, onset is about 0.74. GWb for substorms triggered by external (solar wind) factors and 0.67 GWb for non-triggered substorms. The open magnetic flux generally drops following the onset of triggered substorms but continues to increase for non-triggered events. We interpret this behavior as an indication that the rate of opening of closed field lines on the dayside can exceed that of the nightside reconnection after the onset in non-triggered substorms. By contrast, flux closure is more efficient while the flux opening rate drops in the case of externally triggered onset, so that the closure rate exceeds that of field line opening on the dayside. The rate of equatorward displacement is typically ~ 3 deg/hour. It is statistically correlated with the magnitude of the southward Bz component of the IMF measured by the ACE satellite. It is also correlated with transfer functions describing the efficiency of solar wind energy transfer which involve the transverse electric field carried by the solar wind. The equatorward motion may be global, restricted to local time sectors or a combination of both. No nightside local time sector appears favored where the motion of the equatorial boundary would be more pronounced. The maximum displacement of the polar boundary is statistically located around midnight MLT. [less ▲]

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See detailEuroPlaNet is celebrating a very special year
Chatzchristou, E. T.; Nazé, Yaël ULg

Poster (2007)

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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailUltrastructural organization of the nucleolus in reptiles
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailFeasibility of a urine-based DNA methylation assay for early detection of bladder cancer
Renard, Isabelle; Kelly, J.; Collette, Catherine et al

Poster (2007)

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See detailMulti-dectector row computed tomography of the carpal joint in dogs
Cavrenne; Bolen, Géraldine ULg; DeBusscher et al

Poster (2007)

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See detailDevelopment of urine-based DNA methylation assay for prostate cancer screening
Vener, T. I.; Derecho, C.; Varde, S. et al

Poster (2007)

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate ... [more ▼]

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate specific antigen (PSA) levels are the current standards for PCa early detection. However, serum PSA testing lacks both sensitivity and specificity, and core biopsies frequently fail to identify small foci of PCa. The availability of non-invasive diagnostic molecular tests that could allow for a more precise identification of malignant prostate cells in asymptomatic men would be of great clinical value to improve PCa diagnosis. Study design: 114 men scheduled to undergo a prostate biopsy were enrolled in the study. The biopsies were triggered either by an abnormally high PSA value or by suspicious findings on DRE. Patients with other known or suspected urinary malignancy were excluded from the study. Morning, post-prostate massage and post-biopsy urine samples were collected from all individuals. The main goals of this study were a) to determine if prostate massage can improve the prostate DNA quantity compared to urine collected in the morning or after biopsy, and b) to evaluate the methylation status of a gene panel in urine samples from subjects with cancer found in prostate biopsy tissue cores versus subjects without cancer. Methods: Gene promoter methylation is associated with prostate cancer and has been successfully used for the molecular detection of neoplasia in urine. We have developed real-time methylation specific PCR assays to define the methylation status of several genes. Results: Median age of the patients was 65 years (range 48-85). PCa was found in 51% of the patients. Histological diagnosis of the biopsies was compared to methylation results in urine from 102 samples (89% success rate due low DNA yields for 12 samples). The comparison between different urine sampling techniques showed that prostate massage is needed. The best results were obtained in post massage urine samples with a combination of GSTP1, p14, p16, RARβ2 and RASSF1A resulting in a sensitivity of 74% and a specificity of 75%. Future: A multiplex assay using the Cepheid SmartCycler™ II platform is under development. Further studies are in progress to validate the assay across multiple centers. [less ▲]

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

Detailed reference viewed: 6 (1 ULg)