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See detailDonnées épidémiologiques sur la santé bucco-dentaire chez des enfants scolarisés en région liégeoise
GEERTS, Sabine ULg; Gueders, Audrey ULg

Poster (2011, October)

La prévalence carieuse chez les enfants fait l’objet de nombreuses publications émanant de l’OMS. Ainsi, nous disposons de nombreuses données épidémiologiques issues de divers pays et concernant la santé ... [more ▼]

La prévalence carieuse chez les enfants fait l’objet de nombreuses publications émanant de l’OMS. Ainsi, nous disposons de nombreuses données épidémiologiques issues de divers pays et concernant la santé bucco-dentaire des enfants. Néanmoins, force est de constater que peu d’études ont été réalisées en Belgique et de par ce fait, les données concernant la maladie carieuse et les besoins de traitement chez les enfants Belges restent très limitées. Une étude pilote a été menée par l’équipe du Service de dentisterie conservatrice de l’université de Liège afin de tenter de pallier à ce manquement. L’objectif de cette enquête clinique était de répertorier les facteurs de risques de la maladie carieuse et de recenser les épisodes carieux (présents et passés) chez des enfants âgés entre 6 et 13 ans. Un dépistage clinique a été mené dans 2 écoles de la région liégeoise et a intéressé 306 enfants. D’une part, nos résultats ont montré que la sévérité de l’indice CAO/D et de l’indice cao/d répondaient parfaitement aux objectifs fixés par l’OMS en 1981 (CAO/D < 3) mais qu’ils étaient loin de rencontrer ceux fixés pour 2020 (CAO/D < 1,5). D’autre part, il ressort de notre enquête que peu de jeunes patients sont indemnes de caries (16 %). En effet 84 % des patients examinés avaient déjà présenté un ou plusieurs épisodes carieux. Toutefois, nos résultats tendent à montrer que la maladie carieuse est significativement plus fréquente en denture de lait qu’en denture définitive (p < 0,001). En conclusion, notre étude pilote n’apporte aucun élément neuf dans la connaissance du processus carieux et de son dépistage mais elle a au moins le mérite de planter les premiers jalons qui conduiront à une meilleure connaissance de l’état de santé bucco-dentaire chez des enfants scolarisés en région liégeoise. [less ▲]

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See detailThe energy the auroral electrons in Saturn's atmosphere : remote sensing and thermal consequences
Gérard, Jean-Claude ULg; Gustin, Jacques ULg; Grodent, Denis ULg et al

Poster (2011, October)

Saturn’s north aurora has been observed between January and May 2011 with the Hubble Space Telescope. The objective was to collect spatially resolved spectra at the different local time from dawn to dusk ... [more ▼]

Saturn’s north aurora has been observed between January and May 2011 with the Hubble Space Telescope. The objective was to collect spatially resolved spectra at the different local time from dawn to dusk and compare them with laboratory or synthetic spectra. For this purpose, HST was programmed to slew from mid-latitudes through the auroral oval up to above the limb while collecting data in the timetag mode. The spectra show signatures of absorption by hydrocarbons present above the peak of the auroral emission. The amount of absorption and implications in terms of penetration of the auroral electron beam into Saturn’s atmosphere will be discussed and compared with other determinations of the altitude of the aurora. The effects of the auroral heat import on the thermal structure of the atmosphere both at high and low altitudes will be examined in the light of these results. [less ▲]

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See detailDefining Antonymy by studying Co-occurrences in Context (2)
Steffens, Marie ULg

Poster (2011, October)

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Peer Reviewed
See detailDynamic magnetic resonance perfusion: influence of the cardiac cycle on left ventricle and myocardial signal intensity measurements
BROUSSAUD; AOUCHRIA, S; MANCINI, ISABELLE et al

Poster (2011, October)

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See detailCO2 exchange above a cultivated savanna in sudanian region in northern part of Benin (West Africa)
Ago, Expédit Evariste ULg; Agbossou, Euloge; Galle, Sylvie et al

Poster (2011, October)

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See detailAIRE polymorphisms induce variable extent of apoptosis on melanoma-specific CD8+ T lymphocytes.
Conteduca, Giuseppina ULg

Poster (2011, October)

AIRE regulates thymocyte selection by inducing the expression of tissue-restricted self antigens (TRAs) in medullary thymic epithelial cells (mTECs). MAGE antigens are among TRAs regulated by AIRE. Single ... [more ▼]

AIRE regulates thymocyte selection by inducing the expression of tissue-restricted self antigens (TRAs) in medullary thymic epithelial cells (mTECs). MAGE antigens are among TRAs regulated by AIRE. Single nucleotide polymorphisms (SNPs) of AIRE gene have been reported in humans but their impact on repertoire selection of tumor antigen-specific T lymphocytes is unknown. We report here that the rs1800522 SNP of human AIRE gene is present in mice and that the relative T or C allelic variants differently regulate MAGEB2 gene expression in mTECs. The C allelic variant, protective in humans against melanoma, induces lower MAGEB2 expression than the T allele. When mouse mTECs bearing the TT genotype were gene targeted to substitute the T with the C allelic variant, the co-culture of wild type or transgenic mTECs with MAGEB2-specific syngeneic T cells induced reduced extent of apoptosis and increased frequency of MAGEB2-specific viable T lymphocytes in T cells cultured with mTECs bearing the CC with respect to the TT genotype. These findings demonstrate that alternative allelic variants of AIRE SNPs may differently modulate the T cell repertoire specific against melanoma cells and subsequently may be linked to a different level of suceptibility to a neoplastic disease such as melanoma. [less ▲]

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See detailCharacterization of the interaction between nanoformulated drugs and model lipid membranes towards improved drug delivery systems
De Battice, Laura; Frost, Rickard; Sevrin, Chantal ULg et al

Poster (2011, October)

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See detailInvestigation of mevalonate pathway in aphids
Vandermoten, Sophie ULg; Yu, Wenjuan; Chen, Julian et al

Poster (2011, October)

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See detail21st century Antarctic surface mass balance downscaling from global circulation models
Agosta, Cécile ULg; Favier, Vincent; Genthon, Christophe et al

Poster (2011, October)

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See detailMicroorganisms in Karsts: a case study in St Anne cave, Belgium
Carnol, Monique ULg; Willems, Luc ULg; Malchair, Sandrine ULg

Poster (2011, September 30)

Despite the importance of microorganisms as geochemical agents over geological times, their extended metabolic diversity and their essential role in element cycles (i.e. mineral dissolution, precipitation ... [more ▼]

Despite the importance of microorganisms as geochemical agents over geological times, their extended metabolic diversity and their essential role in element cycles (i.e. mineral dissolution, precipitation, oxido-reduction processes), microbial community composition and processes as well as their ecological role in karst environments are poorly known. While little was published on cave-dwelling microorganisms until the early 1990s, it is now recognized that microorganisms may mediate many important mineral transformations, originally considered to be inorganic in nature. Indeed, recent evidence (Northup & Lavoie, 2001) proved the implication of microorganisms in karstification through precipitation and dissolution processes, resulting in the deposition of carbonate speleothems, silicates, iron or manganese oxides, sulphur compounds and nitrates and in the breakdown of limestone walls. In this poster, we review some potential processes and signs of microbial activity in caves. We present results of a study on the microbial diversity in the ‘St Anne’ cave, Belgium. We focused on ammonia-oxidizing bacteria (AOB), which are responsible for the first, acidifying step of the nitrification process. Chemical composition of the water, numbers of cultivable bacteria (free and particle-associated bacteria) and the diversity of AOB were studied in waters and sediments of the ‘Chawresse’ (underground river in St Anne), on the cave wall and in the soil aboveground. The use of molecular techniques, based on direct ADN extractions, provide more detailed information on the microbial diversity of an environment, as culture-based techniques retrieve only about 1% of bacterial species present in the environment. Bacterial counts showed that most cultivable bacteria were associated with suspended particles and that their numbers decreased underground. Molecular analyses revealed the presence of AOB in the karst system. Comparison of aboveground and belowground diversity also indicated the possibility of a specific endokarst AOB community. Further research perspectives will be discussed. <br /> <br /> <br />Northup, D.E. and Lavoie, K.H. 2001. Geomicrobiology of caves: A review. Geomicrobiology Journal, 18(3):199-220. [less ▲]

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See detailL'entrepreneuriat féminin au Maroc: Quelles sont les variables contextuelles qui influencent l'entrepreneuriat?
Salman, Noura ULg; El abboubi, Manal; Henda, Sana

Poster (2011, September 30)

L’objectif principal de cet article est d’explorer les fondements de l’entrepreneuriat féminin au Maroc et de présenter les modèles existants. Ce qui nous amène à poser les questions suivantes : « Quels ... [more ▼]

L’objectif principal de cet article est d’explorer les fondements de l’entrepreneuriat féminin au Maroc et de présenter les modèles existants. Ce qui nous amène à poser les questions suivantes : « Quels sont les variables contextuelles et socio-économiques qui influencent l’entreprenariat au Maroc ? » et « qui sont ses femmes qui ont choisi d’entreprendre au féminin, en bravant courageusement les différents obstacles socio-culturelles qui caractérisent la société marocaine? ». Présenté ainsi, notre recherche entend dresser un état de l’art des différentes variables pouvant expliquer les composantes de l’entrepreneuriat féminin au Maroc et de conceptualiser ses mécanismes opérationnels afin de proposer des pistes de réflexions adaptées au contexte marocain et applicables par les femmes entrepreneures. [less ▲]

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See detailTraçabilité du bois et place des Forêts Communautaires camerounaises au sein du processus FLEGT
Beauquin, Antoine; Doucet, Jean-Louis ULg; Julve Larrubia, Cécilia et al

Poster (2011, September 29)

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See detailEvidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV)
Rodriguez, Sabrina ULg; Trono, K.; Jones, L.R.

Poster (2011, September 26)

Evidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV) Sabrina M. Rodríguez1*, Karina Trono2, Leandro R. Jones3 1 Molecular ... [more ▼]

Evidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV) Sabrina M. Rodríguez1*, Karina Trono2, Leandro R. Jones3 1 Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA), University of Liège (ULg), Belgium. 2 Instituto de Virología, CICVyA, Instituto Nacional de Tecnología Agropecuaria INTA-Castelar, CC 25 (1712), Castelar. 3 División de Biología Molecular, Estación de Fotobiología Playa Unión, CC 15, Rawson, Chubut 9103, Argentina. *E-mail: sabrina.rodriguez@ulg.ac.be El resultado de la infección por el virus de la leucemia bovina (BLV) es diverso. La mayoría de los animales infectados resultan portadores asintomáticos (AL) y cerca del 30% desarrolla una condición benigna denominada linfocitosis persistente (LP). La forma tumoral o linfosarcoma (LS) afecta a un 1-5% de los bovinos infectados. Las bases genético-moleculares del desarrollo de las distintas formas clínicas son desconocidas. Las Repeticiones Terminales Largas (Long Terminal Repeats, LTR) del genoma viral constituyen determinantes genéticos de patogenia en el caso de otros retrovirus. Sin embargo, esta posibilidad no ha sido evaluada para el BLV. Los análisis para probar la correlación entre los caracteres clínicos y genotípicos entre especies deben ser corregidos incluyendo la filogenia del grupo. De otra manera, la historia evolutiva compartida puede comprometer la independencia estadística del análisis. Sobre estas bases, el objetivo de este trabajo consistió en estudiar la influencia de las variaciones genéticas de las regiones regulatorias LTR del BLV en el desarrollo de las diferentes formas clínicas de la infección mediante métodos comparativos filogenéticos, cladísticos y probabilísticos. Con este fin, se secuenció la región 5´LTR de 40 provirus obtenidos a partir de bovinos naturalmente infectados con BLV que presentaron las diferentes formas clínicas (AL, PL, LS). Fueron identificadas siete posiciones polimórficas que mostraron una asociación aparente con la presentación clínica. Una reconstrucción de la filogenia del grupo fue realizada a partir de las secuencias de la región env obtenidas para 28 de los 40 provirus estudiados en este trabajo. En conjunto, los análisis comparativos cladísticos y probabilísticos basados en el alineamiento empírico de las secuencias y la filogenia del grupo sugieren que las posiciones 41 y 56 del 5´LTR podrían estar correlacionadas con la presentación clínica. Los análisis probabilísticos indicaron además una asociación con la patogénesis viral para las posiciones 373, 450, 494 y 505, aunque los soportes estadísticos correspondientes fueron menores en comparación con los soportes obtenidos para las posiciones 41 y 56. Estas observaciones indican que las regiones LTR del BLV podrían constituir determinantes de patogenia. [less ▲]

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See detailNeuropeptides and endogenous opioids release after acute inflammation
Chavarria Bolanos, Daniel ULg; Flores-Reyes, Hector; Pozos-Guillen, Amaury

Poster (2011, September 24)

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See detailBiocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on apple
Kwasiborski, Anthony ULg; Renaut, Jenny; Lepoivre, Philippe ULg et al

Poster (2011, September 21)

The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to ... [more ▼]

The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to apply biological control methods. Pichia anomala strain K was previously identified as an efficient biocontrol agent of the main apple pathogens, Botrytis cinerea and Penicillum expansum. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A cDNA-AFLP and gene disruption study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala strain Kh6 (a haploid form of P. anomala strain K displaying the same biocontrol properties). However, these studies suggested also implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing the triple interaction, host/pathogen/antagonist and the proteomic tool allowing to study the ultime expression of the genome without a priori. One 50mm wound per apple were covered by a membrane and inoculated by a P. anomala suspension then by B. cinerea or not. Samples were collected during the exponential and stationary phase to identify the early and later responses to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced by the presence of B. cinerea in exponential and stationary phases were identified by MALDI-ToF. One hundred five and sixty spots of proteins were influenced by the presence of B. cinerea in exponential and stationary phases respectively. In exponential phase, P. anomala Kh6 in absence of B. cinerea uses mainly the glycolysis pathway, whereas in presence of pathogen, it orientates its energetic metabolism to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new orientation, P. anomala Kh6 probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea and prevents the use of nutrients by the pathogen. In stationary phase, no differences in the P. anomala Kh6 energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala Kh6 seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate. [less ▲]

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See detailMicrostructure characterisation of nanocomposite polymeric foams by X-ray microtomography
Plougonven, Erwan ULg; Marchot, Pierre ULg; Detrembleur, Christophe ULg et al

Poster (2011, September 21)

Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon ... [more ▼]

Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon nanotubes inside a polymer matrix shows exceptional electromagnetic interference shielding effectiveness when foamed. However, the effective properties of such materials strongly depend on the shape and topology of the microstructural cells. An accurate method for investigating the cellular microstructure is X-ray microtomography (XRµT), for it is non-destructive, and it provides 3D geometric information. Although it cannot be used to observe nanofiller dispersion, it has a strong potential for cell structure characterization. In order to reduce the need for trial and error for tailoring these materials, our objective is to characterize, using XRµT, two different types of foaming procedures, namely supercritical CO2 batch foaming and freeze drying. As the resolution is limited compared to cell size, we have developed a novel statistical method based on 3D autocorrelation to determine characteristic length and examine anisotropy. We present results for these two types of foams and show the limitations of this method. [less ▲]

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See detailDriven weak to strong pinning crossover in a partially nanopatterned superconductor
Shaw, Gorky ULg; Banerjee, S. S.; Tamegai, T. et al

Poster (2011, September 20)

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See detailA robustness study to investigate the performance of parametric and non-parametric tests used in Model-Based Multifactor Dimensionality Reduction Epistasis Detection.
Mahachie John, Jestinah ULg; Gusareva, Elena ULg; Van Lishout, François ULg et al

Poster (2011, September 19)

Model-Based Multifactor Dimensionality Reduction (MB-MDR) is data mining technique to identify gene-gene interactions among 1000nds of SNPs in a fast way, without making assumptions about the mode of ... [more ▼]

Model-Based Multifactor Dimensionality Reduction (MB-MDR) is data mining technique to identify gene-gene interactions among 1000nds of SNPs in a fast way, without making assumptions about the mode of genetic interactions. By construction, one of the implementations of MB-MDR involves testing one multi-locus genotype cell versus the remaining cells, hereby creating two imbalanced groups for trait distribution comparison. To date, for continuous traits, we have adopted a standard F-test to compare these groups. When normality assumption or homoscedasticity no longer hold, highly inflated results are to be expected. The power and type I error control of MB-MDR under these assumptions has been thoroughly investigated in Mahachie John et al [1]. The aim of this study is to assess, through simulations, the effects of ANOVA model violations on the performance of Model-Based Multifactor Dimensionality Reduction (MB-MDR). We quantify their effect on MB-MDR using default options, but at the same time introduce alternative options with increased performance. The better handling of imbalanced data using robust approaches [2] within a MB-MDR context is exemplified on real data for asthma-related phenotypes. 1. EJHG (2011), Early view 2. David Freedman, Statistical Models: Theory and Practice, Cambridge University Press (2000), ISBN 978-0521671057 [less ▲]

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See detailComparison Of Different Methods For Detecting Gene-Gene Interactions In Case-Control Data
Cattaert, Tom ULg; Rial Garcia, J. A.; Gusareva, Elena ULg et al

Poster (2011, September 19)

It is generally believed that epistasis makes an important contribution to the genetic architecture of complex disease, and numerous statistical and bioinformatics methods have been developed to detect it ... [more ▼]

It is generally believed that epistasis makes an important contribution to the genetic architecture of complex disease, and numerous statistical and bioinformatics methods have been developed to detect it. We compare several state-of-the-art epistasis detection methods in terms of empirical power, type-I error control, and CPU time. The methods compared include Model-Based Multifactor Dimensionality Reduction (MB-MDR) [1, 2], BOolean Operation-based Screening and Testing (BOOST) [3], EPIBLASTER [4], Random Jungle (RJ) [5], Logistic Regression and PLINK. Our comparative study is based on an extensive simulation study using different two-locus models, exhibiting both main effects and epistasis [3]. In these simulations, 100 SNPs are generated, no LD between them. All genotypes are assumed to be in Hardy-Weinberg equilibrium. Furthermore, 2 disease-associated SNPs are selected, with MAFs set to 0.1, 0.2 and 0.4. The MAFs of the non-disease associated SNPs are uniformly distributed on [0.05, 0.5]. In order to achieve high accuracy in empirical power estimation, all simulation settings involve 1000 replicates. All methods are applied to WTCCC Crohn's Disease data. [1] Calle, M.L. et al. (2008), Tech. Rep. No. 24, Dep. of Systems Biology, Univ. de Vic [2] Cattaert, T. et al. (2011), Ann. Hum. Gen. 75, 78-89 [3] Wan, X. et al. (2010), Am. J. Hum. Gen. 87, 325-340 [4] Kam-Thong, T. et al. (2011), Eur. J. Hum. Gen. 19, 465-471 [5] Schwartz, D.F. et al. (2010), Bioinf. 26, 1752-1758 [less ▲]

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See detailGenome-wide epistasis screening for Crohns’ disease
Gusareva, Elena ULg; Van Steen, Kristel ULg

Poster (2011, September 19)

Genome-wide association (GWA) studies of Crohn's disease have identified numerous genes. However, a substantial portion of the heritability of this disease remains unexplained. Some gene variants, not ... [more ▼]

Genome-wide association (GWA) studies of Crohn's disease have identified numerous genes. However, a substantial portion of the heritability of this disease remains unexplained. Some gene variants, not detectable via main effects GWA study, may manifest themselves only in interaction with other variants. To search for interacting genes involved in the regulation of Crohn's disease, we performed GWA epistasis screening in a large human cohort (1851 cases/2938 controls) belonging to the Wellcome Trust Case Control Consortium (WTCCC). All subjects were genotyped with the GeneChip 500K Mapping Array Set (Affymetrix chip). SNPs that passed our quality control (359,479 SNPs) were processed in Biofilter (a software package that looks for candidate epistatic genes contributing to disease risk) giving rise to 14,185 SNPs. Subsequent MB-MDR epistasis screening discovered four pairs of interacting SNPs on chromosome 4q35.1 and eight pairs on chromosome 11q23.2. The identified pairs of SNPs were confirmed with synergy-based measures. Notably, despite their mapping to the same genomic regions, the interacting SNPs were not in LD (r^2 < 0.5). Our findings support the idea of close chromosomal localization of two pairs of interacting genes that are involved in development of Crohn's disease. [less ▲]

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See detailParameter estimation using slug tests : application to a heat injection and storage experiment
Vandenbohede, Alexander; Louwyck, Andy; Daoudi, Moubarak et al

Poster (2011, September 19)

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See detailInsertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Godaux, Damien ULg; Emonds-alt, Barbara; Cardol, Pierre ULg et al

Poster (2011, September 18)

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases ... [more ▼]

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization. [less ▲]

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See detailInfluence of the processing parameters on the physicochemical properties of puff pastry margarines
Lefebure, Emilie ULg; Ronkart, Sébastien; Brostaux, Yves ULg et al

Poster (2011, September 18)

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See detailHow a topic treatment can worsen the situation: A new type of iatrogenic "burn".
REMY, Christophe ULg; LAUNGANI, Alexis; MAGNETTE, André ULg et al

Poster (2011, September 15)

Many studies showed the high frequency of cutaneous intolerances to ketoprofen but in 85 percent of them, these are photoallergies. The Belgian dematologists published an article a few years ago ... [more ▼]

Many studies showed the high frequency of cutaneous intolerances to ketoprofen but in 85 percent of them, these are photoallergies. The Belgian dematologists published an article a few years ago, demonstrating that it is important to avoid prescription of topical ketoprofen during sunny periods. Our experience showed the risks of lesions without exposing to UV radiations. These gels, on free sale, are widely used by the public. In physiotherapy, they are also regularly used by practitioners who rarely know the risk of photoallergies and often ignore the possibility of burn. In France, many questions have already been asked about these gels and they were even the object of a temporary withdrawal. [less ▲]

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See detailRapidly solidified microstructure of 3D parts fabricated by selective laser melting (SLM) - Examples of stainless steel 316L and titanium Ti-6Al-4V
Contrepois, Quentin ULg; Lecomte-Beckers, Jacqueline ULg

Poster (2011, September 12)

Laser additive manufacturing process capable of producing fully dense metallic parts direct from 3D CAD know a fast development. Major concerns are made to achieve the best accuracy of the final geometry ... [more ▼]

Laser additive manufacturing process capable of producing fully dense metallic parts direct from 3D CAD know a fast development. Major concerns are made to achieve the best accuracy of the final geometry and the reduction of the residual stresses but metallurgical aspects are also essential. The process can be described as a succession of very small welds. A key in the optimization of the mechanical properties is the understanding of the specific solidification mechanisms. Microstructures of two alloys were studied by metallographic examination and EBSD analysis. [less ▲]

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See detailDOES INCREASING LEVEL OF CONDENSED DISTILLERS SOLUBLES (PROTIWANZE®) SUPPLEMENTATION AFFECT MILK PRODUCTION IN DAIRY CATTLE ?
Lessire, Françoise ULg

Poster (2011, September 08)

INTRODUCTION The objective of this study was to evaluate whether introducing the condensed distillers solubles Protiwanze® (PW) at different concentrations in dairy cows diet induces changes in milk ... [more ▼]

INTRODUCTION The objective of this study was to evaluate whether introducing the condensed distillers solubles Protiwanze® (PW) at different concentrations in dairy cows diet induces changes in milk production. MATERIAL AND METHODS Three herds (72 Holstein and 25 Brown Swiss, DIM = 101 ± 57; daily milk production = 34.90 ± 8.40 L) received successively two RTM differing in PW content for 4 weeks, including a 7-day transition: 0% and 10% on a dry matter basis for Herd 1 and 10% and 15% for Herd 2 and 3. Both concentrations were distributed alternatively and repeated twice, each cow being its own control. Rations were formulated to meet energy and nitrogen requirements. During each testing period, animals were scored (Body Condition, Rumen Fill, Fecal Consistency, Undigested Fraction according to Zaaier (2001)) and their milk production and composition were measured by the Dairy Herd Improvement. RESULTS AND CONCLUSION Increasing the level of PW did not significantly change milk production in any Herd: Values were for Herd 1 at PW0%: 33.08 ± 0.76 L, at 10%: 31.66 ± 0.68 L, for Herd 2 at PW10%: 32.85 ± 0.93 L and at 15%: 32.37 ± 0.89, for Herd 3 at PW10%: 28.71 ± 1.21 L and at 15%: 31.80 ± 1.16. In Herd 1, milk urea decreased from 243.80 ± 4.55 mg/L for PW0% to 218.05 ± 4.03 mg/L for PW10%, (p<0.01), while fat percentage increased from 3.38% ± 0.09 (PW0%) to 3.77% ± 0.08 (PW10%) (p<0.01). Concerning scores, unconclusive changes were observed. Although increasing level of PW in dairy cows diet did not change milk production or composition, it is profitable since PW is 30-40% cheaper than other protein supplements like soya and canola meals. Moreover, it allowed to lower milk cost price by 1,5 eurocent per liter, when used at higher concentration. [less ▲]

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See detailEffect of steam explosion pre-treatment on enzymatic saccharification of lignocellulosic material
Meyer, Laurence ULg; Jacquet, Nicolas ULg; Vanderghem, Caroline ULg et al

Poster (2011, September 08)

Taking into account the sharp rise in prices and the depletion of resources of petroleum, an alternative to fossil resources is needed. A probable alternative is the use of lignocellulosic raw material to ... [more ▼]

Taking into account the sharp rise in prices and the depletion of resources of petroleum, an alternative to fossil resources is needed. A probable alternative is the use of lignocellulosic raw material to produce biofuels. The “first generation” biofuels are highly controversial because of the use of food plant material. The aim of the “second generation” biofuels is to take lignocellulosic non-food plant material as raw material. Lignocellulosic biomass has a very complex structure made of linkages between lignins, cellulose and hemicelluloses. The saccharification of these lignocellulosic materials requires the fractionation of its constituents. Research has lead to many lignocellulosic biomass fractionation pre-treatments. This study particularly focuses on the steam explosion pretreatment followed by an enzymatic saccharification. Steam explosion is a thermomechanical process which allows the breakdown of the lignocellulosic material structure by the combined action of steam heating, hydrolysis induced by the organic acids formed during the process and shear stress resulting from the pressure rough drop. This treatment leads to modification of the physical parameters such as water retention capacity, cristallinity rate of the cellulosic fraction, hydrolysis of the hemicellulosic fraction and rearrangement in the lignin structure. Such modifications are supposed to make cellulose enzymatic hydrolysis from complex lignocellulosic material easier. In order to verify this hypothesis, different lignocellulosic raw materials have been pre-treated by steam explosion. These materials were sugar beet pulp, corn straw and miscanthus. In order to check the effect of steam explosion pre-treatment on cellulose, a microcrystalline cellulose was also treated. Steam explosion was performed at a vapor pressure of 18 bars and with a retention time of 2 minutes. The steam exploded lignocellulosic materials and the untreated one were submitted to a hydrolysis with a mixture of enzymes composed of cellulases and cellobiase activities during 24 hours. The quantification of glucose in the hydrolysates at different times was performed by HPAEC-PAD. Rate of cellulose converted into glucose were better with steam exploded raw material showing that steam explosion allows improvement of lignocellulosic material for enzymatic saccharification. [less ▲]

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See detailDoes feeding dairy cattle with different levels of condensed distillers solubles (Protiwanze®) increase the risk of Sara?
Lessire, Françoise ULg; Rollin, Frédéric ULg

Poster (2011, September 08)

INTRODUCTION The objective of this study was to evaluate the effects of different levels of Protiwanze® (PW) supplementation, a highly acid (pH = 3.8 ± 0.8) and fermentescible CDS, on ruminal function of ... [more ▼]

INTRODUCTION The objective of this study was to evaluate the effects of different levels of Protiwanze® (PW) supplementation, a highly acid (pH = 3.8 ± 0.8) and fermentescible CDS, on ruminal function of dairy cows. MATERIAL AND METHODS PW supplementation was tested in 5 dairy herds (144 cows, DIM: 96 ± 61, daily milk production: 34.69 ± 8.22 L). In Herd 1, TMR was supplemented with 0% or 10% of PW on a dry matter basis for 4 weeks including a 7-day transition period. Each concentration was distributed twice during 2 periods alternatively with the other one, each cow being its own control. PW concentrations were 10 and 15% in Herd 2, 3 and 4. During every period, milk production was measured by the Dairy Herd Improvement and ruminal fluid sampled by a stomach tube (Ruminator®) on 5 cows. Samples were assessed for pH (portable pH meter), redox potential (Methylene blue test) and protozoa (optical microscopy). RESULTS AND CONCLUSION Ruminal pH values ranged between 5.94 and 7.74. Even when a correction factor of 0.5 was applied to take into account possible saliva contamination, only 8 pH samples pleaded for SARA although protozoa and methylene blue tests were within norms and cows clinically normal. No significant correlation between pH value, milk production and fat content could be demonstrated. Ruminal pH did neither significantly differ between the different levels of PW supplementation. In conclusion, in this study, PW could be used in dairy cows TMR at a level as high as 15% without increasing the risk of SARA. [less ▲]

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See detailMICRO-H2 – Microbiological production of hydrogen: study of microalgal and bacterial processes
Calusinska, Magdalena ULg; Joris, Bernard ULg; Wilmotte, Annick ULg et al

Poster (2011, September 07)

The project MICRO-H2 aims to study and exploit the microbial (bacterial and algal) production of hydrogen (H2). In addition to building a competence centre around the H2 production by microorganisms and ... [more ▼]

The project MICRO-H2 aims to study and exploit the microbial (bacterial and algal) production of hydrogen (H2). In addition to building a competence centre around the H2 production by microorganisms and the molecular monitoring of the processes, this project tries to address two main socio-economic issues. First, transport and many economic activities will be based on hydrogen energy in the near future. Secondly, many researches and technology developments deal with renewable resources. Therefore, a new integrated technology for a sustainable development should be promoted. Photofermentation and dark-fermentation are the most promising ways to produce biohydrogen. The main advantage of the first process is the complete conversion of substrate, if any, to hydrogen. However, present H2-production rates by microalgae remain low. Therefore, a better understanding of the microalgal hydrogen metabolism and rate improvements by genetic engineering are needed. On the other hand, dark-fermentation achieves at present far higher H2-production rates, but improvements are expected through monitoring and optimisation of bacterial diversity and activity. The objectives about bacterial H2 production were to increase knowledge, stability potentialities and investigation skills about the consortia of bacteria involved in bioreactors treating wastewater rich in carbohydrates to produce biohydrogen. The project focused mainly on the study of the potentialities of different consortia, with a focus on Clostridium strains. Concerning the microalgal production of H2, the objectives were to increase knowledge on the metabolic interactions that determine H2 evolution at the cellular level and to produce new strains with increased ability for H2 production in the two-stage process. [less ▲]

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See detailMastitis risk indicators assessed through a germ specific epidemiological model in southern Belgium
Theron, Léonard ULg; Sustronck, Bart; Bertozzi, Carlo et al

Poster (2011, September 07)

Bovine mastitis is the most prevalent pathology in dairy production; in order to identify herd related risk indicators, our study focused on germ-specific approach to refine known risk indicators between ... [more ▼]

Bovine mastitis is the most prevalent pathology in dairy production; in order to identify herd related risk indicators, our study focused on germ-specific approach to refine known risk indicators between environmental or contagious epidemiologic models. We gathered clinical mastitis during 3 months in 21 walloon dairy farms representing 1630 Holstein cows (mean: 67; SD: 18). Farmers were interrogated by a questionnaire about their main practices and basic mastitis knowledge questions. Quarters and severity were recorded, sampled for bacteriology at day 0 and day 21 during 3 months. Individual cell counts were also recorded. Risk analysis included Odds ratio calculation (OR) and multivariate regression. The average prevalence of mastitis on the study was 3.25% (min 0.3%, max : 11.2%, SD : 2.8%) which is coherent with previous epidemiological studies in Wallonia. 124 quarters were sampled and non-negative bacteriology shown 45% of strictly environmental pathogens, 22% of strictly contagious pathogens and 33% of mix epidemiological model pathogens. Overall risk assessment revealed that cubicles free-stall herds shown mastitis lower incidence (OR = 0.49; IC95 [0.34-0.72]). Surprisingly, post-dipping appeared as an overall positive risk indicator for mastitis prevalence (OR =2.13; IC95 [2,13-3,39]). The germ specific approach revealed that Post-dipping was present in low incidence herds with contagious models (OR=0.29; IC95 [0.12-0.71]) and that the type of pathogen model is an interaction in post-dipping risk analysis (Breslow-Day test p<0.0001), whereas housing had no interaction with the model (p=0.33). In this way of categorizing variables, one is able to identify overall risk indicators or germ-specific model. This can lead to more accurate advices in farm consulting. [less ▲]

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See detailA single synthetic small molecule that generates force against a load
Lussis, Perrine ULg; Svaldo-Lanero, Tiziana; Bertocco, Andrea et al

Poster (2011, September 06)

Biological molecular machines are able to rectify random thermal motions to generate directional force and carry out tasks on both the molecular and macroscopic length scales1. Although some artificial ... [more ▼]

Biological molecular machines are able to rectify random thermal motions to generate directional force and carry out tasks on both the molecular and macroscopic length scales1. Although some artificial nanomachines have been synthesized2-4 and used to collectively carry out mechanical tasks5-8, the direct measurement of the mechanical processes at the single molecule level has yet to be realized. We show that biased Brownian motion of the sub-molecular components in a hydrogen bonded [2]rotaxane9-a molecular ring threaded onto a molecular axle-can be harnessed to generate significant directional forces. We applied a mechanical load to the ring by atomic force microscopy (AFM) cantilever during single molecule pulling-relaxing cycle. Using fluctuation theorems, we were able to relate the measurements of the work done at the level of individual molecules to the free energy change measured previously by ensemble measurements. The results show that individual rotaxane molecules can generate directional forces of similar magnitude to biological machines, and extend the capabilities of AFM-based single molecule mechanics to the world of small molecules. 1. Schilva, M. (ed.) Molecular Motors (Wiley-VCH, Weinheim, 2003). 2. Kinbara, K. & Aida, T. Toward intelligent molecular machines : Directed motions of biological and artificial molecules and assemblies. Chem. Rev. 105, 1377-1400 (2005). 3. Kay, E. R., Liegh, D. A. & Zerbetto, F. Synthetic molecular motors and mechanical machines. Angew. Chem. Int. Ed. 46, 72-191 (2007). 4. Browne, W., Feringa, B. L. Making molecular machines work. Nature Nanotech. 1, 25-35 (2006). 5. Berna, J., Leigh, D. A., Lubomska, M., Mendoza, S. M., Pérez, E. M., Rudolf, P., Teobaldi, G. & Zerbetto, F. Macroscopic transport by synthetic molecular machines. Nature Mater. 4, 704-710 (2005). 6. Liu, Y., Flood, A. H., Bonvallet, P. A., Vignon, S. A., Northrop, B. H., Tseng, H.-R., Jeppesen, J. O., Huang, T. J., Brough, B., Baller, M., Magonov, S., Solares, S. D., Goddard, W. A., Ho, C.-M. & Stoddart, J. F. Linear artificial molecular muscles. J. Am. Chem. Soc. 127, 9745-9759 (2005) 7. Eelkema, R., Pollard, M. M., Vicario, J., Katsonis, N., Ramon, B. S., Bastiaansen, C. W. M., Broer, D. J. & Feringa, B. L. Molecular machines: Nanomotor rotates microscale objects. Nature 440, 163 (2006). 8. Muraoka, T., Kinbara, K., Aida, T. Mechanical twisting of a guest by a photoresponsive host. Nature 440, 512-515 (2006). 9. Kay, E. R. & Liegh, D. A. Hydrogen bond-assembled synthetic molecular motors and machines. Top. Curr. Chem. 262, 133-177 (2005). [less ▲]

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See detailPOLLEN DISPERSAL ABILITIES OF ALS TARGET-SITE RESISTANT BLACK-GRASS (ALOPECURUS MYOSUROIDES HUDS.)
Maréchal, Pierre-Yves ULg; Henriet, François; Vancutsem, Françoise ULg et al

Poster (2011, September 05)

Target-Site Resistant (TSR) black-grass individuals were introduced in a field, at different growing stages to be synchronised with autochthonous population. Patches of susceptible black-grass were ... [more ▼]

Target-Site Resistant (TSR) black-grass individuals were introduced in a field, at different growing stages to be synchronised with autochthonous population. Patches of susceptible black-grass were created by setting protective canvas on few areas (from 0,5 up to 32m²), during herbicide spraying and they were removed afterwards. Those patches were disposed along rays starting from the introduction point at distances from 2 to 128 meters. In those patches, local black-grass grew regularly and was able to receive some pollen from the resistant individuals, located in the centered emitting area. The herbicide field treatment (mesosulfuron+iodosulfuron : 15+3 g.a.i.ha-1) was efficient, confirming that the local population is susceptible to ALS inhibitors, was preceded by another herbicide treatment targeting only broad-leaves weeds, performed on the entire field, including patches, such as the rest of cultural practices such as fertilization, and fungicide and insecticide treatment. All seeds were harvested in each patches separately and tested in glasshouse. Seed samples were sprayed with sulfometuron, herbicide active ingredient known not to be affected by non-target site resistance, in a way to detect the percentage of resistant black-grass that can be engendered after the introduction of TSR individuals and to determine the distance resistant pollen can cover. DNA analyses were performed on surviving plants to confirm the presence of the mutation. [less ▲]

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See detailGlucose-responsive layer-by-layer microcapsules as self-regulated insulin delivery system
Alaimo, David ULg; Detrembleur, Christophe ULg; Auzély-Velty, Rachel et al

Poster (2011, September 03)

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See detailDisulfide bridges, new prospect in drug delivery systems?
Cajot, Sébastien ULg; Danhier, F.; Schol, Daureen ULg et al

Poster (2011, September 03)

Detailed reference viewed: 31 (6 ULg)
See detailSmart block copolymers for biomedical applications
Sibret, Pierre ULg; Schol, D; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2011, September 03)

Stimuli-responsive polymers are polymers that respond with rapid changes to external stimuli such as pH, temperature, light or ionic strength. Responses to the stimuli may manifest themselves as changes ... [more ▼]

Stimuli-responsive polymers are polymers that respond with rapid changes to external stimuli such as pH, temperature, light or ionic strength. Responses to the stimuli may manifest themselves as changes in solubility, shape or surface characteristics. They can also lead to the fomation of micelles or a sol-gel transition. These materials are very intersesting for different biomedical applications such as drug delivery systems, tissue engineering or sensors. In this work, we focused on two separate systems: on the one hand, micelles and, on the other hand, iron oxide nanoparticles. These nanoparticles are generally synthesized in a one-step process by alkaline coprecipitation of iron (II) and iron (III) precursors in aqueous solutions (Massart process). However, iron oxide nanoparticle suspensions produced by Massart process are not stable enough in physiological conditions to be used as is. A stabiliser coating is needed to avoid aggregation and consequent precipitation of the colloids in body fluids. For this coating, the polymer blocks chosen are: the poly(ethylene oxide) (PEO), the poly(acrylic acid) (PAA) and the poly(N-isopropyl acrylamide) (PNIPAM). The high flexibility and hydrophilicity of PEO chains make it an outstanding candidate for confering stealthiness to micelles and nanoparticles in order to avoid their rapid removal from the body by the opsonization process. The PAA is the pH-responsive block and the anchoring block. The PNIPAM is the thermoresponsive block with a thermal transition close to 37°C (99°F). Triblock copolymer was synthesized by a Reversible Addition Fragmentation Transfer Polymerization (RAFT) process combining poly(acrylic acid) PAA, poly(N-isopropylacrylamide) and poly(ethylene oxide) or poly[acrylate methoxy poly(ethylene oxide)]. This triblock copolymer was used alone to form micelles and with iron oxide to make magnetic stabilized nanoparticles. The behaviour of micelles and coated nanoparticles was investigated in different conditions by a combination of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential measurements. Moreover, PAA-b-PNIPAM-b-PAMPEO nanofibers were obtained using electrospinning technique. These nanofibers present interesting prospects in the field of biomaterials and biomedical applications as they mimic the extracellular matrix of the skin. [less ▲]

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See detailVoice Impact of a Prolonged Reading Task at Two Intensity Levels: Subjective Self-Ratings
Remacle, Angélique ULg; FINCK, Camille ULg; Roche, Anne et al

Poster (2011, September 01)

Objectives: The objectives of this study are to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading, using subjective self-ratings. Methods: Fifty ... [more ▼]

Objectives: The objectives of this study are to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading, using subjective self-ratings. Methods: Fifty normophonic women undergo two sessions of voice loading in which the required intensity level of voice varied: 60-65 dB(A) for the first session, and 70-75 dB(A) for the second session. Voice intensity level is constantly controlled with a Digital Sound Level Meter at a distance of 40cm from the mouth. Subjects’ voices are orally loaded by reading a novel of their choice for 2 hours in a quiet room (background level < 30dBA). The effects of loading are assessed with regard to self-ratings of voice obtained before and every 30 minutes during the loading tasks. The following parameters are evaluated using a 100-mm horizontal visual analogue scale: voice quality, phonation effort, vocal fatigue and laryngeal discomfort. A repeated-measure analysis of variance (ANOVA Duration X Intensity Level) where the subjects are used as their own controls is carried out to compare data obtained at different times of the reading sessions. Results and conclusion: There is a significant effect of the duration (p < 0.0001) for all the subjective self-ratings, but no differences between the two intensity levels of reading. Feeling of phonation effort, vocal fatigue and laryngeal discomfort significantly increase during prolonged reading, whereas self-rating of voice quality decrease. Subjects do not report more complaints in the 70-75 dB session than in the 60-65 dB one. To conclude, subjects’ self-ratings of voice significantly worsens throughout the reading task as a duration effect, but there is no intensity level effect. [less ▲]

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See detailAutomated synthesis of [18F] FBEM for labeling of thiol containing compounds
Paris, Jérôme ULg; Thonon, David ULg; Kaisin, Geoffroy ULg et al

Poster (2011, September 01)

[18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the ... [more ▼]

[18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the present work was to develop a fast, reproducible and fully automated synthesis of this compound in order to improve its availabilty as well as for obvious radioprotection matters. [less ▲]

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See detailA method for evaluating weaknesses and critical steps in the Radiation Treatment Process through Precursor Events reporting
LENAERTS, Eric ULg; DELGAUDINE, Marie ULg; COUCKE, Philippe ULg

Poster (2011, September)

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains ... [more ▼]

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains, functional Basic Responsibilities and levels of Severity of precursor events [less ▲]

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See detailOptimized deposition of photocatalytic TiO2 thin films produced by a non-aqueous sol-gel method
Malengreaux, Charline ULg; Timmermans, Adrien; Pirard, Sophie ULg et al

Poster (2011, September)

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See detailSeagrass response to in situ trace element contaminations
Richir, Jonathan ULg; Luy, Nicolas; Lepoint, Gilles ULg et al

Poster (2011, September)

The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of ... [more ▼]

The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of knowledge regarding to that seagrass contamination and decontamination kinetics. For the first time, Posidonia were experimentally in situ contaminated in 2009, at 10m depth in Calvi Bay (Corsica), by a mix of 15 TE (Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Ag, Cd, Pb and Bi). Trace element concentrations were measured by DRC-ICP-MS in leaves, rhizomes and in overall shoots sampled at regular time intervals. P. oceanica immediately accumulated pollutants from the beginning of experiments; once contaminations ended, TE concentrations came back close to their original levels within 2 weeks, or at least showed a clear decrease. Posidonia leaves exhibited different uptake kinetics for many elements (Cr, Cu, Zn, Ag, Cd, Pb and Bi): the younger growing leaves incorporated chemicals more rapidly than the older ones. Rhizomes did not show any clear trend, except for Cu, Zn and Bi. These results demonstrate that Posidonia is a very sensitive sentinel to immediately delineate punctual pollutions similar to what might be measured in contaminated Mediterranean waters. The very good response of Posidonia leaves to pollutant short term expositions suggests their routine use in regularly scheduled monitoring programs. Nevertheless, to by-pass Posidonia leaves deciduous character and their capability to detoxify rapidly, long term accumulation recordings also necessitate below-ground tissues analyses. [less ▲]

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See detailNEONATAL INVASIVE GROUP B STREPTOCOCCAL (GBS) INFECTIONS IN EUROPE
MELIN, Pierrette ULg; Berner, Reinhard; Afshar, Baharak et al

Poster (2011, September)

Objectives: To describe clinical characteristics and capsular type of GBS isolates responsible of invasive infections in infants from Belgium, Bulgaria, Czech-Republic, Denmark, Germany, Italy, Spain and ... [more ▼]

Objectives: To describe clinical characteristics and capsular type of GBS isolates responsible of invasive infections in infants from Belgium, Bulgaria, Czech-Republic, Denmark, Germany, Italy, Spain and United Kingdom, representing one of the main objectives of the DEVANI (DEsign of a Vaccine Against Neonatal Infections) project. Methods: Surveillance of invasive GBS infections in infants was performed from mid-2008 through December 2010. For each case, a standardized case report form was filled. Samples from cases were processed using local procedures. GBS isolates were characterised in national central labs using standardised type-specific (Ia, Ib-IX) latex agglutination and molecular typing methods. Results: Data on 188 infants with invasive infection were analysed: 144 (60.6%) early onset diseases (EOD) and 74 (39.4%) late onset diseases (LOD). In EOD, mean/median ages at onset were 14/0 hours and the male:female ratio was 1.25. The predominant manifestation at onset was respiratory distress (42% cases); 83% cases were associated with sepsis/bacteremia, 15% with pneumonia and 6% with meningitis. Late-prenatal screening cultures were obtained from 51% of cases’ mothers and only half of these were positive for GBS. Non-elective C-section, intrapartum fever and rupture of membrane (>18h) were more frequent in EO-cases’ mothers versus healthy babies’ GBS-positive mothers. The major serotypes were III (43%), V (21%) and Ia (18%). In LOD, mean/median ages at onset were 42/34 days and the male:female ratio was 0.9. The predominant characteristic at onset was fever (62% cases); 70% cases were associated with sepsis and 30% with meningitis. Very rare manifestations were osteomyelitis and cellulitis. Serotype III was highly predominant (80.6%) followed mainly by Ia (12.5%). Death rates were 4.7/1.5% in EOD/LOD. Conclusions: Clinical presentations were associated with age at onset of infection. Serotype III predominated in neonatal infections. Prenatal screening was not universal neither sensitive. Study funded through the European Commission Seventh Framework. [less ▲]

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See detailEffect of Deferoxamine Mesylate on Freezability of Blood Supplemented Canine Semen
Van Den Berghe, Femke ULg; Rijsselaere, Tom; Van Soom, Ann et al

Poster (2011, September)

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See detailStructural and morphological Control of TiO2 nanoparticles by selective adsorption of organic molecules
Dufour, Fabien ULg; Durupthy, Olivier; Cassaignon, Sophie et al

Poster (2011, September)

Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde ... [more ▼]

Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde (TiO2), is one of the most interesting material in many applications, such as photocatalysis, photovoltaics, photo/electrochromics or sensors. [1] In addition to an influence of the nanoparticles size, recent works on the anatase phase demonstrated the effect of the expressed surfaces nature on the nanoparticles photoreactivity. [2] Sol-gel method has been used to obtain a wide range of anatase nanoparticles sizes and morphologies by the control of concentrations, ions in solution, solution acidity and aging parameters. [3] In order to avoid the brookite phase often obtained with the anatase phase, microwave heating was efficiently used. Furthermore, conventional heating method has been compared with hydrothermal and microwave activated synthesis. The precise morphological control has been achieved by selective adsorption of organic molecules during synthesis: different shapes of anatase nanoparticles have been synthesized. [4,5]. The influences of synthesis parameters and the organic molecules nature on the nanoparticles structure and morphology have been analysed with various techniques, such as XRD and HRTEM. [less ▲]

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See detailEnzymatic Interesterificationof Palm oil and Fractions: A Calorimetric Study
Danthine, Sabine ULg; De Clercq, Nathalie; Lefebure, Emilie ULg et al

Poster (2011, September)

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See detailMolecular effective quantification of pathogens and total flora in meat products
Adolphe, Y.; Crevecoeur, Sébastien ULg; Delhalle, Laurent et al

Poster (2011, September)

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See detailDevelopment of a quantitative risk assessment for cheese made from raw goat milk contaminated by Listeria monocytogenes
Delhalle, Laurent ULg; Ellouze, Mariem; Clinquart, Antoine ULg et al

Poster (2011, September)

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal ... [more ▼]

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal Agency for the Safety of the Food Chain in 2005. The source of the contamination was related to a shedder goat, excreting 2.6 log cfu (colonies forming units) L. monocytogenes / ml without any clinical symptom. On the basis of the collected data, a quantitative microbial risk assessment model was developed covering the production chain from the milking of goats until the consumed products. Predictive microbiology models were used to simulate the growth of L. monocytogenes during the process of cheeses made from goat raw milk. The modular exposure assessment model showed a significant growth of L. monocytogenes during chilling and storage of the milk collected the day before the cheese production (increase of 1.7 log cfu/ml for the median) and during the step of starter and rennet adjunction to milk (increase of 0.8 log cfu/ml for the median). The median estimated final result (in the fresh cheese) was equal to 3.5 log cfu/g. The model estimates (expressed as median final result issued from the exposure assessment) were realistic compared to the number of L. monocytogenes measured in the fresh cheese (3.6 log cfu/g) reported during the cheese contamination period. The average number of expected cases of human listeriosis was between 0 and 1 for a high-risk sub-population and 0 for a low-risk healthy sub-population. Scenario analysis was finally performed to identify the most significant factors and aid in developing priorities for risk mitigation. Thus, by using quantitative risk assessment and predictive microbiology models, this study provided valuable information to identify and to control critical steps in a local production chain of goat cheese made from raw milk. [less ▲]

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See detailGroßer Lebensmittel-assoziierter Ausbruch durch rekombinante Noroviren GII.g/GII.1 in einem süddeutschen Krankenhaus
Hoffman, Dieter; Mauroy, Axel ULg; Protzer, Ulrike et al

Poster (2011, September)

a) Einleitung Noroviren sind derzeit die häufigsten Gastroenteritiserreger. Durch ihre niedriger infektiöse Dosis und Umweltstabilität verursachen sie häufig Ausbrüche in Krankenhäusern und anderen ... [more ▼]

a) Einleitung Noroviren sind derzeit die häufigsten Gastroenteritiserreger. Durch ihre niedriger infektiöse Dosis und Umweltstabilität verursachen sie häufig Ausbrüche in Krankenhäusern und anderen Gemeinschaftseinrichtungen. Wir berichten über den größten Norovirusausbruch in einem Münchner Krankenhaus der vergangenen Jahre mit über hundert infizierten Patienten und insgesamt 28 betroffenen Mitarbeitern aus verschiedenen Bereichen. b) Material/Methoden Wir wiesen Noroviren GII durch real time Amplifikation eines 94 bp am 3´Ende des Nichtstruktur Polyprotein nach. Sieben positive Proben, aus verschiedenen Bereichen des Klinikums einschließlich zweier Außenstellen, sowie Proben von 2 Küchenmitarbeitern wurden sequenziert. Dabei untersuchten wir 1110 bp, bestehend aus Polymerase- und Capsidsequenzen. Das Ausbruchsmanagement bestand aus einer task force, die neben dem ärztlichen Direktor aus Ärzten, Pflegekräften, Hygieniker und Virologen Apotheke, Einkauf, und Küchenbereich bestand. Zu den Maßnahmen gehörten unter anderem Abfrage aller betroffenen Stationen mit mindestens täglicher Aktualisierung der line-list, Umstellen desinfizierender Maßnahmen, sehr eng kontrolliertes Patientenmanagement (Verlegungen nur nach Rücksprache mit der Hygiene), Begehung aller betroffenen Stationen und der Küche, Abfrage der Nahrungsmittelaufnahme durch Fragebögen, Fortbildung des Personals und enge Kooperation mit den zuständigen Behörden. c) Ergebnisse Am 15.06. traten bei Patienten erstmalig Übelkeit und Erbrechen auf, am Folgetag wurde bei den ersten Patienten Noroviren in Stuhlproben nachgewiesen. Die letzte Neuinfektion wurde am 23.06. registriert, so dass der Ausbruch bereits am 04.07. in Absprache mit dem RGU München für beendet erklärt wurde. Insgesamt wurde Norovirus RNA bei 116 Patienten auf 34 Stationen sowie 28 Mitarbeitern nachgewiesen. Zweiundzwanzig Mitarbeiter waren in der direkten Patientenversorgung tätig, eine Mitarbeiterin arbeitete im Laborbereich. Fünf der Mitarbeiter waren im Küchenbereich beschäftigt und sind damit die wahrscheinlichste Infektionsquelle. Sie gaben keine bzw. nur wenig ausgeprägte Symptome an, mit denen sie weiterarbeiteten. In den bisher untersuchten Lebensmittelrückstellproben konnten keine Noroviren detektiert werden. Alle untersuchten Sequenzen entsprachen einem rekombinanten GII.g/GII.I Stamm. Die ähnlichsten publizierten Sequenzen unterscheiden sich nur durch 17 Nukleinsäure-, und 3 Aminosäureaustausche d) Schlussfolgerungen Wir beschreiben hier den größten Norovirenausbruch in Münchener Kliniken der letzten Jahre. Er breitete sich rasch aus und trat außerhalb der üblichen Norovirussaison auf. Durch schnelle Einleitung strikter Hygienemaßnahmen und einer engen Überwachung der betroffenen Stationen fanden wir nach 10 Tagen keine Neuinfektionen und konnten den Ausbruch zeitnah für beendet erkären. Erstmalig wiesen wir den rekombinanten Stamm GII.g/II.1 in unserem Universitätsklinikum nach. Fehlende oder niedrige Immunität der exponierten Personen könnte die initial rasche Ausbreitung begünstig haben. Zusammenfassend zeigen unsere Daten, dass bei Gastroenteritisfällen auch in den Sommermonaten mit Noroviren gerechnet werden muss. Diese können auch von oligo- oder asymptomatischem Personal ausgehen. Schnelle Erregerdiagnose und entsprechende Hygienemaßnahmen sind wesentlich in der Bekämpfung der Infektion. [less ▲]

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See detailEstimation du débit de filtration glomérulaire en transplantation rénale: étude multicentrique d'évaluation de la performance de la cystatine C
Masson, I.; Maillard, N.; Jaafar, A. et al

Poster (2011, September)

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See detailTissue Proteomics of Epithelial Ovarian Cancers.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, September)

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See detailMALDI Mass Spectrometry Imaging for the Screening of Epithelial Ovarian Cancer Biomarkers.
Boyon, Charlotte; Longuespée, Rémi ULg; Kerdraon, Olivier et al

Poster (2011, September)

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See detailA new longirostrine ichthyosaur (Reptilia) from the Toarcian of France broadens the ecological diversity of the genus Temnodontosaurus
Martin, Jeremy; Fischer, Valentin ULg; Vincent, Peggy et al

Poster (2011, September)

The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids ... [more ▼]

The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids were occupying the lower trophic levels. We describe here an almost complete skeleton of this successful genus from the middle Toarcian (Lower Jurassic) of the Beaujolais foothills near Lyon, France, and assign it to a new species of Temnodontosaurus. This specimen exhibits cranial peculiarities such as a thin, elongated, and likely edentulous rostrum, as well as a reduced quadrate. Such morphological combination indicates dietary preferences that markedly differ from other species referred to as Temnodontosaurus. Despite a conservative postcranial skeleton, we propose that Temnodontosaurus is one of the most ecologically diverse genera of ichthyosaurs, including apex predators, small and soft prey longirostrine hunters, and generalized forms. Ammonites collected along the described specimen indicate that the new species is younger (bifrons ammonite zone) than most known Toarcian ichthyosaurs and therefore slightly postdates the severe environmental changes and marine invertebrate extinctions that occur during the Toarcian Oceanic Anoxic Event. The present study hence raises the question whether the speciation of Temnodontosaurus towards a new ecological niche, may have been a consequence of the post-crisis marine ecosystem reorganization. [less ▲]

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See detailKinetics and diffusional limitations in nanostructured heterogeneous catalyst with controlled pore texture
Pirard, Sophie ULg; Hermans, Sophie; Devillers, Michel et al

Poster (2011, September)

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See detailSystematic expression profiling of Hox genes in the central nervous system of adult mouse
Theys, Nicolas; Doshishti-Agolli, Konstantin; Coste, Cécile ULg et al

Poster (2011, September)

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See detailMolecular histology for epithelial ovarian cancers biomarker hunting: new issues for biology and pharmacology.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, September)

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See detailPili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project)
Imperi, Monica; Rinaudo, Daniela; Creti, Roberta et al

Poster (2011, September)

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI ... [more ▼]

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI (Design of a Vaccine Against Neonatal Infection) project. Methods GBS isolates from pregnant women (PW) and cases of newborn infection (NI) were collected in 8 European countries (Belgium, Bulgaria, Czech Republic, Denmark, Germany, Italy, Spain, United Kingdom) during 2009/10 under the auspices of DEVANI. Total no. of strains examined was 1078 and 192 from PW and NI, respectively. Isolates were screened by multiplex PCR and FACS analysis to evaluate respectively gene presence and surface-exposure of pili. Results The most common gene patterns found were PI-2a alone, PI 1+2a and PI 1+2b, while the PI-2b gene alone was very rare. The most prominent result was that a majority of isolates from NI carried the PI-1+2b gene pattern, while the most common pattern among PW was PI-1+2a. Most of analyzed strains express at least one pilus on their surface. Conclusions All isolates contained at least one gene coding for pili. When present pili 2a and 2b were highly surface exposed. [less ▲]

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See detailAssessing the impacts of technical uncertainty on coupled surface/subsurface flow model predictions using a complex synthetic case
Wildemeersch, Samuel ULg; Goderniaux, Pascal ULg; Orban, Philippe ULg et al

Poster (2011, September)

According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful ... [more ▼]

According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful management tools in this context since they can predict system responses to future stresses. However, numerical modelling at such a scale faces specific issues linked to (1) the representation of the geological and hydrogeological complexity, (2) the uneven level of characterisation knowledge, (3) the representativity of measured parameters and variables in the field, and (4) the CPU time needed for solving the numerical problem. Assumptions and simplifications made for dealing with these issues can lead to a series of models differing by their complexity and by the reliability of their predictions. Consequently, modellers have to find a compromise between complexity and reliability. The main objective of this research is to estimate the impacts of technical uncertainty, which is the uncertainty related to the numerical implementation, on groundwater flow model predictions. To reach that objective, the methodology consists in comparing reference predictions (hydraulic heads and flow rates) of a complex and close to reality synthetic case with the predictions provided by a series of simplified models (coarse spatial discretisation, coarse time discretisation, simplified law in the unsaturated zone). The synthetic case reflects the main characteristics found in groundwater bodies of South Belgium (Condroz region of Wallonia), characterised by a succession of limestone synclines and sandstone anticlines. The numerical model is developed with the fully-integrated surface/subsurface flow and transport code HydroGeoSphere using a mesh refined along the surface water network (153027 nodes and 269872 elements). A 5-year reference transient simulation, with daily stress factors is performed. The simulated hydraulic heads and flow rates constitute the reference observations and predictions for the comparison with the simplified models. The simplified models tested differ by their horizontal (500 m vs. 1000 m element size) and vertical (8 layers vs. 3 layers) spatial discretisations, their time discretisation (daily vs. monthly stress factors), and the type of constitutive law used for simulating the unsaturated flow (linear vs. van Genuchten). The models are run with the same parameter values than those used in the reference model to evaluate the deterioration in model predictions due to technical uncertainty. Additionally, some of the models are calibrated with the inverse modelling code PEST to distinguish how far a model calibration can possibly compensate for technical uncertainty. Then, predictions from each simplified model are compared with the reference predictions of the synthetic case. Then, the simplified models are ranked using several model performance criteria. Results of this research provide guidelines for the numerical implementation of groundwater flow models at the water body scale with respect to specific groundwater management objectives. [less ▲]

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See detailTail amputation in a Belgian blue bull
Lamain, Guillaume ULg; Sartelet, Arnaud ULg; Touati, Kamal ULg

Poster (2011, September)

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See detailEnhancement of in-situ Transformation of M2C Eutectics Carbides during High Temperature Heat Treatment on a HSS and a Semi-HSS Grades
Tchuindjang, Jérôme Tchoufack ULg; Sinnaeve, Mario; Lecomte-Beckers, Jacqueline ULg

Poster (2011, September)

Alloys of the complex system Fe-Si-C-Cr-C-X, where X is a strong carbide forming-element are well known to exhibit interesting mechanical properties, including wear and abrasion resistances. Such a ... [more ▼]

Alloys of the complex system Fe-Si-C-Cr-C-X, where X is a strong carbide forming-element are well known to exhibit interesting mechanical properties, including wear and abrasion resistances. Such a tribological behavior is mainly due to the presence of carbides especially those obtained during the solidification route and that are known as primary or eutectic carbides. It may therefore be interesting to determine the relative stability of primary carbides when considering thermal and thermomechanical treatments performed at a temperature high enough to allow either the homogenization of the matrix or the occurrence of a desired grain size. This thermal stage is often required to produce tailored microstructures that can lead to improved mechanical properties. In this work a series of thermal treatments performed on samples originated form casting foundry parts were done. Raw materials are both HSS and semi-HSS grades used in application where wear resistance is needed. Thermo- Calc® (TC) simulations and Differential Thermal Analysis (DTA) were performed to determine the crystallization behavior and the subsequent solid state transformations of the studied alloys respectively in equilibrium and in non equilibrium conditions. Light and Scanning Electron Microscopies were done together with hardness measurements in order to enhance metallurgical features of the heat treated samples. Image analysis yielded the determination of carbides volume fractions. It appears from microstructural analyses and carbides quantification that Mo-rich eutectic carbides undergo in situ phase transformations during heat treatments. In fact Mo-rich M2C carbides transform themselves into MC, M6C and M3C, through a so-called budding phenomenon. Such a phenomenon is the evidence of a preferential migration of some atoms that escape from the parent M2C carbide to diffuse further away from their initial site with increasing time and temperature. The stable or metastable nature of eutectic carbides is also discussed from DTA and TC results, as M2C carbides found in both as-conditions and DTA samples were not predicted by equilibrium conditions. [less ▲]

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See detailPerinatal depression in adolescent mothers : a public health concern
Noirhomme-Renard, Florence ULg; Tancredi, Annalisa; Gosset, Christiane ULg

Poster (2011, September)

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See detailEnzymatic interesterification of palm oil and fractions: monitoring the degree of interesterification using different methods.
De Clercq, Nathalie; Danthine, Sabine ULg; Nguyen, Mai et al

Poster (2011, September)

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See detailImpurity fingerprints for the identification of counterfeit medicines - a feasibility study
Sacré, Pierre-Yves ULg; Deconinck, Eric; Daszykowski, Michal et al

Poster (2011, September)

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See detailMicrobiological and chemical characterisation of St Anne cave, Belgium
Carnol, Monique ULg; Malchair, Sandrine ULg

Poster (2011, September)

In Belgium, most drinking water is provided by calcareous karst aquifers. Chemical and microbiological characterisation of these systems focalises mainly on the transfer of pollutants and microbial ... [more ▼]

In Belgium, most drinking water is provided by calcareous karst aquifers. Chemical and microbiological characterisation of these systems focalises mainly on the transfer of pollutants and microbial contaminants, major sources of sanitary risks. These studies are generally based on bacterial cultures, representing however only 1% of bacterial species present in the environment. Molecular techniques allow the study of the global microbial diversity of an environment, as they are based on direct ADN extractions, without previous culturing steps. The objective of this research was the study of the microbial diversity in the ‘St Anne’ cave, Belgium. Chemical composition of the water, cultivable bacteria and the diversity of ammonia-oxydizing bacteria (AOB) were studied in waters and sediments of the ‘Chawresse’ (underground river in St Anne), on the cave’s wall and in soils aboveground. Bacterial counts revealed that most cultivable bacteria were associated with suspended particles and that their numbers decreased underground. Molecular analyses revealed the presence of AOB in the karst system. AOB are responsible for the first, acidifying step of the nitrification process. Further studies will specify and quantify their activity in this karst system. [less ▲]

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See detailTetanus following castration in a buck
Lamain, Guillaume ULg; Guyot, Hugues ULg

Poster (2011, September)

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See detailSuccessful reduction of cholelithiasis in a Holstein cow
Lamain, Guillaume ULg; Frisee, Vincent ULg; Ramery, Eve ULg et al

Poster (2011, September)

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See detailAnt-aphid mutualism - A question of microorganisms?
Fischer, Christophe ULg; Patris, Geoffrey; Duriaux, Adrien ULg et al

Poster (2011, September)

It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection ... [more ▼]

It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of view, this phenomenon has been well studied, but its chemistry and semiochemical mechanisms are still largely unknown. This study aims to identify semiochemicals involved in the establishment of this relation and their sources, using both chemical and behavioral methods. Bioassays revealed that the greatest part of ant attraction toward aphid colonies is due to honeydew volatile compounds; enabling ant scouts to find more quickly aphid colonies and distantly recognize myrmecophilous species. Many of those VOCs seeming to have microbial origins, the main honeydew microorganisms have been isolated and their roles in VOCs production and ant attraction have been investigated. [less ▲]

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See detailSedimentology of a continuous Givetian-Frasnian carbonate succession in Sauerland (Germany) and MS comparison with the time-equivalent ones of Ardennes (Belgium) and Moravia (Czech Republic)
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Boulvain, Frédéric ULg et al

Poster (2011, September)

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick ... [more ▼]

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick) covers a well constraint stratigraphic interval starting at the base of the Givetian (Stritzke 1991;Aboussalam et al., 2003) and according to our new datations ending within the Lower Famennian. The Middle-Upper Devonian shelf-edge within the Rheinisches Schiefergebirge can be traced from the supposed position along the southern rim of the Dinant Syncline and the Eifel Synclines, northwards along a line connecting the southern margin of the Devonian reefal outcrops of Attendorn and Brilon (Krebs 1967, 1974). The depositional setting of the investigated section corresponds to complex slope and basinal environments where reworked material from the proximal Brilon platform (located to the north) and basin deposits coexist. Thus, this section allows to follow the evolution of the Givetian-Frasnian Brilon (e.g., Machel 1990; Stritze 1990, 1991) platform in a deeper setting. Petrographic analysis of more than 300 thin-sections leads to the identification of 9 microfacies which are integrated into a palaeoenvironmental model. Microfacies curve evolution shows two main trends. A shallowing upward trend ending within a typical proximal slope setting (dismantling of the platform) followed by a deepening upward trend which is characterized by several meter of pelagic mudstone within the upper part of the studied section. Magnetic susceptibility variations in sedimentary rocks, have commonly been interpreted as related to variations of detritic inputs through climatic or sea level changes (Crick et al., 1994). The magnetic susceptibility (MS) study of more than 330 samples from this long-time fore-reef carbonated succession is an opportunity to better constrain our sedimentological interpretations. To do so, we propose a comparison between general MS trends and some parameters such as microfacies and relative sea level fluctuations interpreted on the basis of the sedimentological study. The relatively long stratigraphic interval covered by the Burgberg section offers a good opportunity to compare our data with the time equivalent Devonian sections of the Ardennes (Belgium) and Moravian karst area (Czech Republic) (Boulvain et al., 2010). And thus to test the magnetic susceptibility tool for long distance correlation between stratigraphically well constraint sections. [less ▲]

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See detailEffect of heat treatment on the risks and benefits of consumption of raw cow milk: preliminary results
Cardoen, S; Claeys, W.; De Zutter, L. et al

Poster (2011, September)

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See detailTime and space matter when monitoring faecal pollution : searching for efficient sampling designs in relation to hydrological regimes.
Cauchie, Henry-Michel ULg; Burnet, Jean-Baptiste; Ogorzaly, Leslie et al

Poster (2011, September)

Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event ... [more ▼]

Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event. Therefore, classical monitoring schemes based on low, fixed sampling frequency turned out to inefficiently estimate the microbial water quality. Several studies have therefore been carried out on important drinking water resources (springs and surface reservoir) in order to understand the link between the faecal contamination events and the hydrology and the hydrology of these waterbodies. Physico-chemical and hydrological variables that could serve as relevant indicators for triggering selective sampling were also investigated. [less ▲]

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See detailEffeti comportamentali dell'endosulfan su girini di Rana dalmatina
Lavorato, Manuela; Bernabé, I.; Tripepi, S. et al

Poster (2011, September)

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See detailJUDE: A Far-UV Imager for JUICE
Grodent, Denis ULg; Bunce, Emma; Bannister, Nigel et al

Poster (2011, August 31)

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See detailWhen MIR spectrometry helps to promote a local and vulnerable breed
Colinet, Frédéric ULg; Dehareng, Frédéric; Dardenne, Pierre et al

Poster (2011, August 31)

The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of ... [more ▼]

The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of calving). Due to its dual purpose type, income generated by both milk and meat is more stable and more flexible in responding to market fluctuations. Registered DP-BB cows are milk recorded (one of the conditions for them to be registered as DP-BB and therefore to get AEM subsidies). Since near 4 years, during routine milk recording, nearly all mid-infrared (MIR) spectra generated at the milk labs and the information of test-day records were collected in a database. Calibration equations using the MIR spectrometry were developed permitting the prediction of several bovine milk components (e.g., fatty acids (FA)). Their application on the MIR spectral database would allow comparing milk composition from 920 DP-BB and 52,497 Holstein cows (selected cows had a proportion of Holstein or DP-BB in their breed composition of at least 90%). On average, each cows had 6 test-day records with MIR spectra in the database. MIR predictions were analyzed using GLM procedure with 5 fixed effects (breed, herd, lactation number, month of test-day recording and lactation stage); values presented are lsmeans (± s.e.) of the breed. Although milk and fat yields were lower for DP-BB, their FA proportions in fat were different from Holstein. Indeed, there are 66.0% (0.2) and 67.6% (0.0) of saturated FA in fat of milk for DP-BB and Holstein, respectively. Furthermore, the DP-BB milk fat was richer in omega-9 (20.7% (0.2) vs. 19.6% (0.0) for DP-BB and Holstein, respectively). Use of MIR predictions may help stakeholders to promote milk and future dairy products from DP-BB. [less ▲]

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