Phenotypic analysis of strains deleted for genes with unknown function: data and general reasoning.

Poster (2001, August)

Age differences, cognitive control, and prefrontal cortex in the process dissociation procedure

Poster (2001, July 18)

Word priming in normal aging with a task that circumvent explicit memory contamination

Poster (2001, July 18)

S phase dependance and involvement of the NF-kappaB activation kinase to NF-kappaB activation by Camptotheci

Poster (2001, July 04)

Use of stable isotopes to delineate amphipod trophic status in the High Antarctic

Poster (2001, July)

Not thinking of the lure or remembering that it was not heard : Effects of aging

Poster (2001, July)

Cellular localization and regulatory properties of VZV Immediate early proteins in neuronal and non-neuronal cells.

Poster (2001, July)

Certification in chicken meat chanel.

Poster (2001, July)

Gut contents and functional morphology of mouthparts as a tool to characterize Antarctic amphipod feeding patterns

Poster (2001, July)

HST-STIS Observations of Jupiter's FUV Aurora During the Cassini-Jupiter Flyby

Poster (2001, June 25)

Jupiter Thermosphere General Circulation Model (JTGCM) : Formulation and Case Studies Incorporating Ion Drag and Joule Heating

Poster (2001, June 25)

Potential Antioxidant properties of Aceclofenac and its Metabolites: Investigation on an in vitro model

Poster (2001, June 22)

Introduction: Recent studies have shown that some steroidal anti-inflammatory drugs (NSAIDs) could exert their actions by multifactorial processes. Among them, the potential antioxidant activity of ... [more ▼]

Introduction: Recent studies have shown that some steroidal anti-inflammatory drugs (NSAIDs) could exert their actions by multifactorial processes. Among them, the potential antioxidant activity of certain NSAIDs towards various reactive oxygen species (ROS) is often suggested and could have pharmacological relevance. Objective: This study was designed to assess the potential antioxidant properties (IC50 values) of aceclofenac and its metabolites (4’OH-aceclofenanc and diclofenac) on three different systems of ROS production, using chemiluminescence (CL) technique with luminal and electron spin resonance (ESR) spin trapping. Material and Methods: Isolated human PMNs (1x106 cells) were activated with 5x10-7 M PMA in the presence of luminal (CL assays) with or without drug addition. For spin trapping experiments, 100 mM DMPO, a radical trapping agent, was added to the reaction milieu containing 6x106 cells/ml. For free-cell experiments, the Fenton’s reagent was used for generation of ·OH and xanthine/xanthine-oxidase system for O2-radicals. The NaOCl-induced CL, amplified by luminal, was used to test the drug effects on HOCl. Results: On the model of PMA-activated PMNs, 4’OH-aceclofenac exhibited the best antioxidant profile (IC50 = 10 µM) while the effect of the parent drug was less pronounced (IC50 = 100 µM). Diclofenac did not inhibit CL response even at the high dose of 1 mM. Quite similar results were obtained on the NaOCl-induced chemiluminescence, where the efficacy of the drug was as follows: 4’HO-ACE (25 µM) > ACE (1 mM) > DICLO (no effect at 1 mM). By ESR technique, 4’HO-ACE also showed an inhibitory effect (501 µM) on the ROS production by PMA-activated PMN as well as on the ·OH production, while ACE (IC50 = 100 µM) was less efficient and DICLO (IC50 = 1 mM) without significant effect. These findings indicate that beside its anti-inflammatory effects, aceclofenac acts as an antioxidant, at least in part, by the way of its metabolite especially 4’HO-ACE. [less ▲]

Study of lipoxygenase activity during storage of potato tubers (Solanum tuberosum L.) in relation with membrane integrity.

Poster (2001, June 06)

Effects of thromboxane A2 receptor antagonists and COX inhibitors on osteogenic sarcome cell-induced platelet aggregation

Poster (2001, June 01)

Conception, synthèse et évaluation pharmacologique de composés structuralement apparentés au cromakalim en tant qu’activateurs des canaux KATP

Poster (2001, June 01)

Synthèse et évaluation enzymatique de nouvelles coumarines en tant qu’inhibiteurs de thrombine

Poster (2001, June 01)

Comparaison de l’effet de deux dérivés de l’acide 6-(acetoxymethyl)-2-oxo-2H-1-benzopyrane-3-carboxylique sur l’inhibition de l’invasion cellulaire in vitro et in vivo

Poster (2001, June 01)

Sum-frequency generation characterisation of the water/polyethylene glycol interface

Poster (2001, June)

Screening of pectolytic microorganisms by near infrared reflectance spectroscopy.

Poster (2001, June)

Déficits mnésiques dans la dépression : Implication des processus contrôlés et leur lien avec le sentiment subjectif de troubles mnésiques

Poster (2001, May 18)

Effet d’amorçage dans une tâche de catégorisation sémantique en présentation subliminale à l’encodage

Poster (2001, May 18)

Electrografting of conducting polymers onoto ITO surfaces

Poster (2001, May 16)

Association between lipoprotein (A) and cardiac troponins in PTCA patients

Poster (2001, May)

Sum-frequency generation characterisation of the water/polyethylene glycol interface

Poster (2001, May)

Transcriptional regulation of the Chlamydomonas alternative oxidase (Aox1) gene studied with a reporter gene construct

Poster (2001, May)

Beside the cytochrome pathway of respiration, mitochondria of higher plants and many microeukaryotes possess an alternative pathway that bypasses complexes III and IV to directly catalyze the oxidation of ... [more ▼]

Beside the cytochrome pathway of respiration, mitochondria of higher plants and many microeukaryotes possess an alternative pathway that bypasses complexes III and IV to directly catalyze the oxidation of the ubiquinol pool by molecular oxygen. No proton motive force is generated during this reaction and free energy is thus lost as heat. The alternative oxidase (AOX), which is the sole enzyme implicated in this wasteful process, is generally subject to both transcriptional and post-translational regulation. — Recently, we have cloned and characterized two cDNAs and the corresponding gene sequences (AOX1 and AOX2) encoding the alternative oxidase in the green alga Chlamydomonas reinhardtii (1). In order to investigate the transcriptional regulation of the AOX1 gene (which is more strongly expressed than AOX2), we fused the AOX1 promoter to the arylsulfatase-encoding ARS reporter gene. Several transformants expressing the ARS protein were selected and characterized at the molecular level. While AOX transcription is induced or at least enhanced by cytochrome pathway inhibitors in fungi and higher plants, our results indicate that the expression of the AOX1:ARS construct remains unchanged following inhibitor addition in Chlamydomonas. Moreover, its expression was not affected by light, acetate as a carbon source and osmotic stress. A five-to-ten-fold stimulation was however achieved by using nitrate instead of ammonium as a nitrogen source. Effects of toxic compounds (oxidative stress agents and heavy metals) are currently investigated. This work was supported by grants from the Belgian FRFC (2.4527.97) and Fonds Spéciaux pour la Recherche dans les Universités. D. Baurain is a FNRS Research Fellow. (1) Dinant, M., Baurain, D., Coosemans, N., Joris, B. and Matagne, R. F. (2001) Characterization of two genes encoding the mitochondrial alternative oxidase in Chlamydomonas reinhardtii. Curr. Genet. (in press) [less ▲]

Effects on cellular invasion of 6-(acetoxymethyl)-2-oxo-2H-1-benzopyran-3-carboxylic acid derivatives

Poster (2001, April 21)

Determination of divinyl ether synthase activity during storage of potato tubers

Poster (2001, April 18)

Prédation exercée par des grands cormorans sur des truites radio-marquées. Observations préliminaires dans un petit cours d’eau en Wallonie.

Poster (2001, March)

IN SITU MEASUREMENTS OF METAZOOPLANKTON PREDATION ON PROTOZOOPLANKTON: USE OF FLUORESCENTLY LABELLED PROTISTS.

Poster (2001, February 04)

The importance of trophic relationships between metazooplankton and protozoans in rivers are not yet well known, but as some authors have shown during the last decade, the predation exerted by ... [more ▼]

The importance of trophic relationships between metazooplankton and protozoans in rivers are not yet well known, but as some authors have shown during the last decade, the predation exerted by metazooplankton on these organisms might be of importance in the transfer of energy from the pico- and nanoplankton to the metazooplankton. Fluorescent carboxylate microspheres of 0.5 µm in diameter were used to label natural protozooplankton. Different combinations of labelling times and concentrations of microspheres were tested to achieve an optimal labelling of the protists (i.e. a maximal proportion of individuals labelled with an identical number of microspheres); the most appropriate labelling conditions were with short incubations (10 minutes) and relatively low microsphere concentrations (1.105 FMP/ml). Live labelled protozooplankton was used to determine ingestion rates of the natural metazooplankton of the river Meuse (Belgium). It could be showed for all abundant species (i.e. the rotifers Brachionus calyciflorus, Brachionus angularis and Keratella cochlearis) that the predation exerted on protozooplankton was high (from 34 to 182 protists.rotifer-1.h-1) in in situ conditions. [less ▲]

Les 3-alkylamino-4H-1,2,4-benzothiadiazine 1,1-dioxydes diversement substitués par un atome de chlore en tant qu’activateurs des canaux KATP

Poster (2001, January 26)

Conception, synthèse et évaluation pharmacologique d’analogues pyridiniques du Nimésulide en tant qu’inhibiteurs potentiels des cyclooxygénases

Poster (2001, January 26)

Intérêt de la procalcitonine dans le diagnostic des broncho-pneumonies liées au respirateur

Poster (2001, January)

Controls on denitrification in a riparian buffer strip in a small headwater catchment in south Belgium.

Poster (2001)

Phenotypic plasticity of cerebellar radial glia

Poster (2001)

Endosymbiont-bearing trypanosomatids: kinetoplast ultrastructural characterization

Poster (2001)

Sex control in Eurasian perch, Perca fluviatilis

Poster (2001)

The origin of transformation of catfish (Teleostei: Siluriformes) palatine-maxillary system: an example of adaptive macroevolution

Poster (2001)

Hensen's cells as precursors of supernumerary OHCs

Poster (2001)

Modeling the Martian ionosphere using the Total Electron Content measurement by the NEIGE/NETLANDER experiment

Poster (2001)

Evaluation of the antitrypanosomal activity of several plants traditionally used in Benin to treat sleeping sickness

Poster (2001)

Recordings and mechanisms of sounds in three Carapidae fishes

Poster (2001)

Beam-gas excitation of Ar III 3s3p5 singlet and triplet terms

Poster (2001)

Beam-gas excitation of Ar III 3s3p5 singlet and triplet terms

Poster (2001)

About a particular metamorphosis in a Carapidae species

Poster (2001)

Identification of several environmental odours with tin oxide sensor array : three years later

Poster (2001)

The paper discusses the ability of recognition models to classify malodours in the environment three years after their calibration, with the same sources releasing odours. Two methodologies applicable ... [more ▼]

The paper discusses the ability of recognition models to classify malodours in the environment three years after their calibration, with the same sources releasing odours. Two methodologies applicable either in the lab or in the field are used. The effect of the long term drift of the sensors is highlighted on the results of principal component analysis and of discriminant function analysis. The paper examines also the applicability of some drift counteraction methods proposed in the literature. Handled with some care, a correction based on the drift direction in the principal components subspace should be applicable to the classification of real odorous sources in the field. [less ▲]

Determination of the response factors of several desulfoglucosinolates used for quantitative analysis of Brassicaceae

Poster (2001)

Quantitative determination of total and individual glucosinolate contents in Brassica samples (rapeseed/canola, broccoli, cabbage, mustard…) is generally realised according to the reference method ISO ... [more ▼]

Quantitative determination of total and individual glucosinolate contents in Brassica samples (rapeseed/canola, broccoli, cabbage, mustard…) is generally realised according to the reference method ISO 9167-1. After extraction, glucosinolates are purified, then desulfated by Helix pomatia sulfatase. The desulfo-glucosinolates (DSGSL) formed are then separated by reverse phase chromatography associated with UV detection (229 nm). As each compound can possess a very different molar extinction coefficient, it is important - on a quantitative point of view - to define accurately relative response factors. The response factors actually used in the official method have been determined experimentally through indirect methods and have been fixed, some years ago, by consensus between the various laboratories who took part in a ring test. They may need to be revised and calculated for a broader panel of glucosinolates. [less ▲]

Thermal admittance spectroscopy of Mg-doped GaN Schottky diodes

Poster (2001)

Polyphénols du Marrube (Marrubium vulgare L.) et évaluation de l'activité anti-inflammatoire sur Cox-1 et Cox-2.

Poster (2001)

MRI of the normal tarsal joint in dogs

Poster (2001)

An Iron-57 Mössbauer Spectral Study of Na1-xLixMnFe2(PO4)3

Poster (2001)