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See detailMorphologie de la mineuse des fruits du safoutier
Poligui, René Noël ULg; Mouaragadja, Isaac; Francis, Frédéric ULg et al

Poster (2010, July)

L’étude de l’entomofaune des associations culturales prenant en compte le safoutier a permis de mettre en évidence des fortes abondances et une diversité des familles entomologiques. Au niveau des ... [more ▼]

L’étude de l’entomofaune des associations culturales prenant en compte le safoutier a permis de mettre en évidence des fortes abondances et une diversité des familles entomologiques. Au niveau des ravageurs majeurs, nous avons caractérisé un lépidoptère (tortricidae) dont les larves minent les noyaux des fruits du safoutier (Dacryodes edulis) et peuvent induire une chute prématurée d’au moins 25% de fruits. Toutes petites, les larves sont blanches et tachetées de points noirs, puis dans leur dernier stade, elles sont roses avec la tête, les plaque anale et prothoracique brunes. La chrysalide se forme dans un cocon de soie blanc. Elle est brune au départ et noir en fin de nymphose, et se caractérise par un crémaster noir proéminent au milieu de deux paires de soie recourbées à leurs extrémités. La nymphose dure au moins 20 jours. L’adulte est un paillon gris terne. Outre le safoutier, ce lépidoptère a été trouvé dans les fruits de Pseudopdondias longifolia. Des investigations ultérieures révéleront certainement d’autres plantes hôtes et permettront d’envisager une lutte biologique efficiente contre cette mineuse des fruits. [less ▲]

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See detailEntomofaune du safoutier (Dacryodes edulis (G. Don) H.J. Lam ; Burseraceae) au Gabon : résultat dune première année d’investigation
Poligui, René Noël ULg; Mouaragadja, Isaac; Haubruge, Eric ULg et al

Poster (2010, July)

L’entomofaune du safoutier (Dacryodes edulis (G. Don) H.J. Lam ; Burseraceae) a été étudié au moyen des pièges jaunes et des observations visuelles, pendant seize semaines au Gabon, dans la province du ... [more ▼]

L’entomofaune du safoutier (Dacryodes edulis (G. Don) H.J. Lam ; Burseraceae) a été étudié au moyen des pièges jaunes et des observations visuelles, pendant seize semaines au Gabon, dans la province du Haut-Ogooué. Cinq postes de piégeages ont été installés à Franceville et cinq autres en périphérie de la ville. Les deux méthodes situent l’évolution des insectes en deux vagues dont la première et la plus importante s’étend sur huit semaines, correspond à la période de floraison de l’arbre et de pleine croissance des fruits. La seconde vague va de l’arrêt de croissance des fruits à leur maturation. Le piégeage révèle que les abondances des insectes ainsi que leur diversité biologique par poste sont plus importantes en milieu rural qu’en milieu urbain. Le contrôle visuel présente des abondances relativement similaires, mais avec une diversité sensiblement plus élevée en milieu rural. Les sites à prédominance de savanes permettent des captures d’insectes plus importantes que ceux des forets. L’abondance est globalement moins élevée au niveau des arbres males pour les deux techniques d’étude. Par contre, les observations visuelles enregistrent les pics les plus élevés sur les arbres femelles, mais le piégeage les situe sur les arbres males. La diversité biologique est significativement mieux exprimée par le piégeage, alors que le contrôle visuel spécifie mieux le niveau et l’évolution des ravageurs. Les Aphididae, les Cicadellidae et les psyllidae constituent les familles numériquement les plus importantes des insectes nuisibles. Les Apidae, les coccinellidae et les halticidae forment les familles majeures des insectes utiles. Les Tortricidae constituent la famille ayant la plus grande incidence économique sur le safoutier. [less ▲]

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See detailA MODEL FOR WHEAT YIELD PREDICTION BASED ON REAL-TIME MONITORING OF ENVIRONMENTAL FACTORS
Dumont, Benjamin ULg; Lebeau, Frédéric ULg; Vancutsem, Françoise ULg et al

Poster (2010, July)

This paper presents the results of a one year preliminary study in which a real time monitoring system was used to feed the STICS soil crop model. As indicated by the statistical criteria (RMSE and model ... [more ▼]

This paper presents the results of a one year preliminary study in which a real time monitoring system was used to feed the STICS soil crop model. As indicated by the statistical criteria (RMSE and model efficiency), the optimization of some wheat crop parameters allows the model to predict the yields with good accuracy for different soil type and different nitrogen application rates. [less ▲]

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See detailComputerized video multitracking of several small translucent fishes (glass eels) at night with fluorescent elastomer tags
Delcourt, Johann ULg; Bolliet, Valérie; Ylieff, Marc ULg et al

Poster (2010, July)

European eels (Anguilla Anguilla), an endangered species with high economic value, exhibit very unusual features in their multistage catadromous life cycle at different life stages. The reproduction of ... [more ▼]

European eels (Anguilla Anguilla), an endangered species with high economic value, exhibit very unusual features in their multistage catadromous life cycle at different life stages. The reproduction of eel takes place in the Sargasso Sea. Leptocephalus larvae use ocean currents (mainly the Gulf Stream) to migrate to European coastal areas, and then metamorphose into glass eels probably as they reach the continental shelf. Glass eel then, enter estuaries to join the fresh waters network. Knowledge about glass eel biology and migration has mainly increased in the eighties, and it is now well established that the activity during the estuary migration depends mainly on tidal cycle, even if lunar and circadian rhythms and/or direct influence of light and tidal coefficient may interact. Different migratory tactics are suspected on the basis of recent laboratory experiments (Bolliet & Labonne, 2008; Bureau du Colombier et al., 2009), notably the existence of individuals swimming only with the current, others swimming with and against the current, and others presenting a weak degree of activity. The propensity to migrate and glass eels energy content would be linked and may result in different migratory behaviours possibly leading to estuarine settlement (Bureau du Colombier et al., 2007; 2009). Unfortunately, few are known about the glass eel swimming behaviours in estuaries, a crucial point to better understand their estuarine migration: such knowledge would help to characterize the migratory tactics and to better understand the link with energy status. The videotracking system [EthoVision Color-Pro 3.1 (Noldus Information Technology)] which allows to measure automatically the movements of individuals represents an interesting tool to progress in this questioning. Nowadays, video tracking to measure fish behaviours is relatively frequent, but is rather rare in the ecological studies. Moreover, tracking a transparent animal, under water current conditions is a real challenge, particularly at night. To detect the individuals, several eels are marked by a fluorescent VIE tag (Visible Implant Elastomer, Northwest Marine Technology Inc.). VIE do no affect growth, survival and behaviour of tagged glass eels. Initially developed to improve the identification of individual tags in mark-recapture studies of aquatic animals, VIE tags are used here for a new extreme application in ethometry: the study of behaviours of transparent animals in weaker luminosity and in the dark by automatic tracking technology. The individual identification is based on the fluorescent color (red, blue, yellow and green) emitted by the VIE tag injected in the muscle mass on the basis of dorsal fin. The detection of the four marks is excellent. It allows measuring the activity (notably path and speed) of each tagged individuals as a function of tidal and nycthemeral rhythms in the same chronotron (circular aquarium simulating the estuarine conditions) and spatial use at a wide time scale from second to week. Another advantage is the possibility of tracked animals to go out the analysed arena without error of identification when it appears again in the analysed zone. This multitracking method by fluorescent VIE tags could be applied to a large number of species (crustaceans, fish, holothurians, amphibians, …). References: Bolliet & Labonne, 2008 Journal of Experimental Marine Biology and Ecology 362, 125-130; Bureau du Colombier et al., 2007 Physiology & Behavior 92, 684-690 ; Bureau du Colombier et al., 2009 Journal of Fish Biology 74, 2002-2013. Acknowledgements: This work was financially supported by FNRS-FRS (National fund of Research, Belgium) (project n°2.4617.08, n°2.4569.06, n°2.4569.10F), by Hubert Curien Tournesol project and by an EGIDE Grant. We thank J-C. Aymes for its help in testing the video tracking software and system. We are also highly grateful to Jacques Rives for its help in settling and monitoring the experiments. [less ▲]

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See detailAssociation des approches morphologique et moléculaire pour l’identification des vers à soies endémiques de Madagascar (Lasiocampidae, Borocera cajani)
Rakotondramanana, Alihasina; Nguyen, Bach Kim ULg; Verheggen, François ULg et al

Poster (2010, July)

La soie est une matière utilisée par les Malgaches depuis bien avant la colonisation. Cette soie est fabriquée à partir de la collecte de cocons des papillons du genre Borocera spp., ver à soie sauvage ... [more ▼]

La soie est une matière utilisée par les Malgaches depuis bien avant la colonisation. Cette soie est fabriquée à partir de la collecte de cocons des papillons du genre Borocera spp., ver à soie sauvage. On connaît une dizaine d’espèces de vers à soie sauvages endémiques à Madagascar. Ces lépidoptères polyphages se nourrissent surtout des feuilles de Uapaca bojeri et d’autres plantes telles que Dodonea madagascariensis et Psidium guyava. Les critères morphologiques se révèlent insuffisant pour identifier cette diversité d’espèces de vers à soie, surtout au niveau des stades immatures. Afin de pallier à cette incapacité d’identifier les espèces, des méthodes de marqueurs moléculaires, en particulier la RAPD (Random Amplified Polymorphic DNA) et iSSR (inter Simple Sequence Repeat), ont été appliquées. En plus de l’identification des espèces, une étude de la diversité et de la distribution des vers à soie suite à la collecte dans plusieurs forêts éloignées de plusieurs de kilomètres les unes des autres (Arivonimamo, Ambatofinandrahana, Antananarivo). Cette étude a été menée dans le cadre de la conservation de ces espèces de vers à soie endémiques en milieu. Afin d’augmenter durablement les populations de vers à soie endémiques dans les forêts de Uapaca, il est important de déterminer les espèces en présence et les capacités de dispersion de ces dernières. Les techniques d’identification moléculaires développées permettront d’orienter les stratégies de gestion des populations de Borocera et des méthodes de gestion de l’écosystème, appropriables par les gestionnaires et les communautés locales des la forêt à Uapaca bojeri. [less ▲]

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See detailNanocoatings of inorganic surfaces by molecular biomimetic
Vreuls, Christelle ULg; Genin, Alexis ULg; Zocchi, Germaine ULg et al

Poster (2010, June 30)

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See detailA green and refillable antibacterial coating for stainless steel
Faure, Emilie ULg; Charlot, Aurélia; Sciannaméa, Valérie et al

Poster (2010, June 29)

Because of its resistance to corrosion and chemicals, relevant mechanical and esthetical properties, stainless steel (SS) is widely used in the daily life (food industry, household appliances, surgery … ... [more ▼]

Because of its resistance to corrosion and chemicals, relevant mechanical and esthetical properties, stainless steel (SS) is widely used in the daily life (food industry, household appliances, surgery …). However, SS is unable to prevent bacteria from adhering, proliferating and forming a resistant biofilm when ageing. Therefore, surface modification is needed for providing durable antibacterial properties. We report here on an all-in-one approach to prepare refillable antimicrobial films using the layer-by-layer deposition of polyelectrolytes. Specifically designed biocidal multilayered polyelectrolyte films that bear 3,4-dihydroxyphenylalanine (DOPA), known as a promoter of adhesion to inorganic surfaces, were deposited onto SS. DOPA was incorporated in the polycationic chains by radical copolymerisation of N-methacrylated DOPA with the quaternary ammonium salt of 2-(dimethylamino)ethyl methacrylate (DMAEMA+). In order to boost the antibacterial activity of the polycationic layer, AgNO3 was added to the aqueous solution of P(DOPA)-co-P(DMAEMA+), which resulted in the in-situ formation of silver based nanoparticles, sources of biocial Ag+. The layer-by-layer deposition of aqueous P(DOPA)-co-P(DMAEMA+)/AgCl/Ag0 suspension and aqueous solution of poly(styrene sulfonate) provides high antibacterial activity against Gram-negative E. Coli bacteria. Moreover, after silver depletion, films retain some antimicrobial activity, thanks to ammonium groups of the copolymer. We also show that the antibacterial activity of the films can then be easily re-boosted. [less ▲]

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See detail(Functionnal) analysis of hydrogen production in Chlamydomonas reinhardtii mitochondrial mutants
Lecler, Renaud ULg; Godaux, Damien ULg; Hamilton, Christopher ULg et al

Poster (2010, June 27)

Mitochondrial Chlamydomonas mutants for respiratory complexes present a decreased dark respiration and apparent yield of photosynthetic linear electron flow. They accumulate reducing power such as NAD(P)H ... [more ▼]

Mitochondrial Chlamydomonas mutants for respiratory complexes present a decreased dark respiration and apparent yield of photosynthetic linear electron flow. They accumulate reducing power such as NAD(P)H and show lower levels of ATP. Under restrictive conditions, like sulfur depletion and anoxia, Chlamydomonas is able to produce hydrogen towards the activation of a chloroplatic O2-sensitive Fe-hydrogenase which catalyses the reduction of electrons to H2. In this study we used an adapted Melis protocol to analyse hydrogen evolution of mitochondrial mutants. For this aim a simple-flask system was built with gaz collecting tubes. A parallel flask was used for GC analyses. [less ▲]

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See detailUnimpaired Implicit Learning abilities in Children with Specific Language Impairment.
Gabriel, Audrey ULg; Fauconnier, Leslie; Meulemans, Thierry ULg et al

Poster (2010, June 26)

Previous studies (Lum et al., 2009; Tomblin et al., 2007; Ullman & Pierpont, 2005) have suggested that difficulties in the procedural learning system could contribute, in part, to the language ... [more ▼]

Previous studies (Lum et al., 2009; Tomblin et al., 2007; Ullman & Pierpont, 2005) have suggested that difficulties in the procedural learning system could contribute, in part, to the language difficulties observed in children with SLI. However, we have recently shown, with a classical serial reaction time (SRT) task, that children with SLI are able to learn implicitly non-linguistic statistical regularities (Gabriel et al., 2010). The aim of the present study was to explore whether children with SLI could learn similar statistical regularities with non-linguistic auditory stimuli. For this purpose, we compared performance of children with SLI and controls in two adapted SRT tasks: a visual SRT task and a non-verbal auditory SRT task. [less ▲]

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See detailAnalogical processes in language development in children with specific language impairment
Leroy, Sandrine ULg; Um, Marelle; Parisse, Christophe et al

Poster (2010, June 26)

Constructivist approach (Goldberg, 1995; Tomasello, 2003) considers that analogical reasoning is a cognitive process which underlies the abstraction of the linguistic forms and the construction of more ... [more ▼]

Constructivist approach (Goldberg, 1995; Tomasello, 2003) considers that analogical reasoning is a cognitive process which underlies the abstraction of the linguistic forms and the construction of more abstract linguistic schemas. When children hear two utterances such as “John eats an apple” and “She sees a bird”, they can infer the abstract schema [Subject Verb Object] by realizing analogies between the two utterances. Morphosyntactic disorders encountered by children with specific language impairment (SLI) could be caused by problems with analogies, what would hinder their abstraction of construction schemas. Consequently, children with SLI would be less productive with their language and would use more fixed linguistic forms. Owing to these problems of generalization, the morphosyntactic development of children with SLI would be slow down [less ▲]

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See detailInfluence of Sport Activity on Trunk Muscle Performances
Grosdent, Stéphanie ULg; Demoulin, Christophe ULg; Coupas, Amandine et al

Poster (2010, June 24)

Introduction: Lumbar pain among athletes is a common problem and can be challenging for athletic trainers and therapists. Unfortunately, little is known about the capabilities of trunk muscle function in ... [more ▼]

Introduction: Lumbar pain among athletes is a common problem and can be challenging for athletic trainers and therapists. Unfortunately, little is known about the capabilities of trunk muscle function in elite athletes Therefore, an objective assessment of muscle performance is relevant in injury prevention and rehabilitation. The purpose of this study was to examine the specific trunk muscle strength and endurance in different sports i.e. swimming, rowing and tennis. Methods: We assessed maximum isometric trunk extensor, rotator, flexor and lateroflexor muscle strength as well as trunk extensor muscle endurance of 10 swimmers, 10 rowers, 10 tennismen and 10 sedentary students (control group). Strength was measured by means of specific trunk dynamometers. An adaptation of the Sorensen test was performed to assess trunk extensor endurance. Results: Muscle strength appeared generally higher among athletes than among control subjects. However, differences were statistically significant only with swimmers for trunk flexors (p<0.05) and trunk rotators (p<0.05), with rowers for trunk flexors (p<0.05) and with tennismen for dominant lateroflexors (p<0.05) and non-dominant trunk rotators (p<0.05). No significant bilateral difference appeared for trunk lateralflexors strength. For trunk rotators, tennismen were significantly stronger in the non-dominant side (p<0.05). Trunk extensor endurance was higher among rowers than among the other groups. Conclusion: This study underlined several differences in trunk muscle performance between athletes and control subjects as well as within athletes groups in relation to their sports. Further studies would clarify the full significance of these results with respect to performance and injury. [less ▲]

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See detailAtypical myopathy in Europe: 2006-2009
Van Galen, Gaby ULg; Atypical Myopathy Alert Group; Votion, Dominique ULg

Poster (2010, June 18)

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See detailDendrochronology to date mettalic reinforcements in roof frames
Maggi, Christophe ULg; Pagès, Gaspard ULg; Hoffsummer, Patrick ULg et al

Poster (2010, June 17)

Studies about metallic reinforcements in historical monuments multiply themselves. Thanks to building archaeology and dendrochronology, we are able to determine the archaeological contexts to which the ... [more ▼]

Studies about metallic reinforcements in historical monuments multiply themselves. Thanks to building archaeology and dendrochronology, we are able to determine the archaeological contexts to which the metallic elements belong and to date them. One of the objectives of the research is to list the different appearances of iron in roof frames and to identify the function of each. At the end, the principal aim is to create a typo-chronological inventory which will index all the iron reinforcements met in medieval roof frames of the Meuse basin (Belgium). [less ▲]

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See detailLa sensibilité à l'anxiété chez l'enfant : Les facteurs de risque et de protection au sein de son milieu familial
Stassart, Céline ULg; Etienne, Anne-Marie ULg

Poster (2010, June 11)

La sensibilité à l'anxiété (AS)est la peur des sensations corporelles liées à l'anxiété. Plusieurs études constatent qu'une grande proportion de la variance de l'AS des filles et la totalité de la ... [more ▼]

La sensibilité à l'anxiété (AS)est la peur des sensations corporelles liées à l'anxiété. Plusieurs études constatent qu'une grande proportion de la variance de l'AS des filles et la totalité de la variance de l'AS des garçons s'expliquent par des facteurs environnementaux. L'objectif est de dégager, chez l'enfant au sein du milieu familial, les facteurs de risque et de protection de la sensibilité à l'anxiété (AS). Dans un premier temps, le lien entre divers états émotionnels des parents et la présence d'une AS chez leur enfant sera étudiée. La part contributive des mécanismes d'apprentissage (transmission d'information, renforcement, modeling) de l'AS lorsque l'enfant ressent des symptômes corporels sera également analysée. Différents effets seront testés: le type d esymptômes sur lequel l'apprentissage porte (douleur/anxiété), la source (père/mère) et la diversité des apprentissages. Dans un second temps, afin d'affiner les liens entre les états émotionnels des parents, les expériences d'apprentissage au sein du milieu familial et la présence d'une sensibilité à l'anxiété chez l'enfant, l'influence de deux modérateurs sera testée: l‘intelligence émotionnelle de l'enfant et les apprentissages sociaux. Les hommes apprennent qu'il est moins acceptable pour eux que pour les femmes d'exprimer leurs craintes. Les mères parlent plus volontiers de leurs inquiétudes et donc, lorsqu'elles présentent des manifestations anxieuses, elles peuvent plus facilement expliquer à l'enfant que ses peurs sont irrationnelles contrairement aux pères qui préféreront ne pas en parler. Que se passerait-il si le père parlait de ses craintes sachant qu'un père qui oserait exprimer sa détresse sera perçu de façon plus marquante par son enfant qu'une mère qui ose le faire? [less ▲]

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See detailIs posterior cerebral hypometabolism always predicitve of dementia in Parkinson's disease?
Deville, Benjamin ULg; Lemaire, Christian ULg; Degueldre, Christian ULg et al

Poster (2010, June 08)

In Parkinson's disease, altered activity in posterior associative cortices has often been associated with dementia. It remains to be determined if this pattern is a reliable marker of a progression toward ... [more ▼]

In Parkinson's disease, altered activity in posterior associative cortices has often been associated with dementia. It remains to be determined if this pattern is a reliable marker of a progression toward dementia in patients without demonstratable dementia. In this retrospective analysis, we used positron emission tomography to study resting-state cerebral fluodeoxyglucose uptake in 8 healthy controls and 8 Parkinson's disease patients who did not have evidence of dementia at the time of assessment. For those patients, clinical follow up was available and we know that they did not meet dementia criteria on average 10,37 years after assessment. The results show that patients had reduced fluodeoxyglucose uptake mostly localised in the right hemisphere and including precuneus, superior temporal, middle temporal and inferior parietal cortices. It also includes right insula. These cerebral activity predominating in posterior cortices is present in non-demened patients but is not always predictive of dementia within the 10,34 years. [less ▲]

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See detailCo‐construction du langage: le rôle de la mère dans les séquences de rectifications
Collombel, Marie; Maillart, Christelle ULg; Caet, Stéphanie et al

Poster (2010, June 07)

Notre recherche a pour but de mieux repérer ce qui semble faciliter chez l'enfant tout venant son développement linguistique et communicationnel, afin de mettre en place des stratégies éducatives adaptées ... [more ▼]

Notre recherche a pour but de mieux repérer ce qui semble faciliter chez l'enfant tout venant son développement linguistique et communicationnel, afin de mettre en place des stratégies éducatives adaptées aux enfants présentant des troubles du langage. Dans une étude longitudinale des interactions mère-enfant chez trois enfants tout-venant entre 1 an 6 mois et 3 ans, notre équipe s'est penchée sur un phénomène interactionnel au croisement de l'appropriation du système linguistique et de son usage : les rectifications. Nos analyses soulignent le rôle clé de la mère ainsi que les similarités et les différences entre les dyades. La sensibilisation à ces dimensions de la communication auprès de parents d'enfants dysphasiques dans le cadre d'un projet de guidance parentale pourrait favoriser la mise en place de formes d'interaction facilitant le développement langagier et communicationnel des enfants. [less ▲]

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See detailImpact of an anxious social situation on emotional facial expressions (EFE) recognition in children
Dethier, Marie ULg; Taskin, Asliane; Blairy, Sylvie ULg

Poster (2010, June 04)

Socially anxious children have difficulties to interact adequately with others. The core characteristic of social anxiety, the fear of being negatively evaluated by others, may among others, be based on ... [more ▼]

Socially anxious children have difficulties to interact adequately with others. The core characteristic of social anxiety, the fear of being negatively evaluated by others, may among others, be based on problems with the decoding of other persons’ emotional facial expression (EFE). Up to now, the research on EFE recognition in socially anxious children has produced mixed results. Whereas some studies reported differences between anxious and healthy children in EFE recognition (e.g., Simonian, Beidel, Turner, Berkes, & Long, 2001), others didn’t find such differences (Melfsen & Florin, 2002). In this study, we addressed two new issues in the investigation of EFE recognition in socially anxious children. Firstly, we investigated self-esteem. Socially anxious children show low confidence in one’s cognitive and social abilities. Furthermore, high self-esteem is related to high capacities of EFE recognition (Garfield, Rogoff, & Steinberg, 1987), and more generally to high level of social functioning (Serretti et al., 1999 ; Shapira et al., 1999). Indeed, the perception of ourselves depends on the way we think others people perceive us. Secondly, past researches have investigated this issue in low anxious situations and thus, not in situations in which social anxious individuals feel threatened. The originality of the present study is that it addresses the relationship between EFE recognition performance and self-esteem in children placed in an anxious social situation. We predicted a low capacity to decode EFE in socially anxious children. Moreover, we hypothesised a relationship between a low self-esteem and difficulties to decode accurately EFE in an anxious social situation. Seventy children (8 – 12 years) were placed in an anxious social situation of performance in which they were instructed to count aloud backwards, beginning at 200 in increments of 13. Children assessed their emotional feeling state, including their degree of anxiety, before and after the anxious social situation. Furthermore, children were assessed on an EFE decoding test consisting of 16 photographs depicting EFE of happiness, anger, disgust, and sadness. For each photograph, they evaluated the presence of nine types of emotions on a 7-point Likert scale. They also completed the Self-Perception Profile for Children (Harter, 1985). No correlations emerged between the accuracy of EFE recognition and an increase of anxious feelings after the anxious social situation. However, self-esteem was correlated with performance on the EFE recognition test, r (70) = -.33, p < .01. Moreover, the lower the child’s level of self-esteem was, the more he/she perceived negative emotions (fear, anger, disgust, and shame) in EFE of anger. In conclusion, social anxiety doesn’t seem to interfere with EFE recognition performance in an anxious social situation. However, low level of self-esteem in children appears to be associated with deficits and interpretative bias in EFE recognition in an anxious social situation. The recognition of the expression of anger, an emotion socially threatening, seems particularly biased in children with low level of self-esteem. [less ▲]

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See detailCapacities of cognitive and emotional empathy in relationship to interpersonal difficulties in alcohol dependant patients (AD)
Dethier, Marie ULg; Blairy, Sylvie ULg

Poster (2010, June 04)

The term empathy refers to two related human abilities: mental perspective taking (cognitive empathy) and the vicarious sharing of emotions (emotional empathy). The main object of this study was to ... [more ▼]

The term empathy refers to two related human abilities: mental perspective taking (cognitive empathy) and the vicarious sharing of emotions (emotional empathy). The main object of this study was to explore the relationship between capacities of both aspects of empathy and their relationships with interpersonal difficulties in alcohol-dependant patients (AD). The research in alcoholism empathy has focalised around one aspect of cognitive empathy - the capacity to infer an emotional state-, and that essentially on the basis of emotional facial expression (EFE) recognition. However, researchers have shown little interest in the investigation of the other aspects of cognitive empathy. The present study focuses on the capacity to infer interpersonal intentions and on emotional empathy. As documented by the research on EFE decoding, AD patients show deficits in cognitive empathy. In this study, we investigated their capacity to infer interpersonal intentions in social situation. We hypothesized that AD patients compared to healthy individuals and to depressed patients will attributed more intentions of reject and of aggressiveness to other people on the basis of their EFE. In this study, emotional empathy was defined as the modulation of the emotional feeling state of the participant in function of the EFE display by other people. We hypothesized that the modulation in AD patient will be different from the one of healthy people in function of the Cloninger subtype of alcoholism (Cloninger, Bohman, Sigvardsson, 1987). This emotional reactivity will be more important in Type II alcoholism and less important in Type I alcoholism. As Mimicry facilitates feelings of empathy in healthy people, it was also investigated. Twenty type I AD patients, 20 type II AD patients, 20 depressed patients, and 20 healthy subjects participated to the study. The alcoholism subtype identification was maid according to the criteria from von Knorring, Bohman, von Knorring, and Oreland (1985). The participant completed questionnaires assessing the quality of interpersonal relationships, their usual quantity of alcohol consumption, and, for AD patients, their level of alcohol dependence. Their capacity to recognise faces was evaluated by the Benton facial recognition test. The empathy tasks were computerized. In the cognitive empathy task, the participants had to evaluate the adequacy (in a 7-point Likert scale) between a film of a face changing from a neutral EFE to an emotional EFE (the photographs come from the material of Matsumoto & Ekman, 1988) and an adjective descriptive of personality. Each adjective was weighted on the interpersonal dimensions of reject, aggressiveness, dominance, and affiliation. In the emotional empathy task, the participants had to evaluate their own emotional feeling state (in a 7-point Likert scale; from very negative to very positive) after watching a series of films depicting emotional faces (same material as before). During this task, the participant’s face was filmed in order to assess mimicry. The differences of empathy capacities between AD patients and control participants (depressed and healthy) are discussed in reference to the characteristics of their interpersonal relationship. [less ▲]

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See detailA proxy tool-box for assessing southern ocean biological carbon pump efficiency.
Dehairs, F.; Cardinal, D.; Cavagna, A.-J. et al

Poster (2010, June)

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See detailInfluence of the matrix on the In Source Decay of permethylated glycans during MALDI-TOF analysis
Smargiasso, Nicolas ULg; De Pauw, Edwin ULg

Poster (2010, June)

Introduction In source decay (ISD) is a common phenomenon occurring very rapidly during ionization process in the source of MALDI-MS instruments and resulting in the presence of well resolved peaks of ... [more ▼]

Introduction In source decay (ISD) is a common phenomenon occurring very rapidly during ionization process in the source of MALDI-MS instruments and resulting in the presence of well resolved peaks of fragments in mass spectrum. While they make interpretation of spectra more complex, these fragments were shown to be useful to sequence peptides and proteins. Concerning glycans, only a few reports were published, using different matrices on various samples and therefore making it difficult to compare. In this context, the goal of this work is to perform a systematic study allowing to define optimal conditions to induce ISD of glycans or, inversely, to minimize this phenomenon in the study of more complex mixtures. Methods Glycans were purchased from Sigma-Aldrich. Iodomethane was used in DMSO/NaOH to permethylate the glycans. This reaction was stopped by water and permethylated glycans were extracted by chloroform. Spectra were recorded on a Bruker Ultraflex II in positive ion mode. 2,5-dihydroxybenzoic acid (DHB) and 6-aza-2-thiothymine (ATT) were prepared at 20 mg/ml in 50 % acetonitrile, 0.1 % formic acid solution. 9-aminoacridine (9-AA) was dissolved at saturation in a 50 % acetonitrile, 0.1 % formic acid and further diluted 4 times in the same solution. α-Cyano-4-hydroxycinnamic (HCCA) acid was prepared at 20 mg/ml in 97 % acetone, 0,1 % formic acid. In some spots, LiI was added to obtain Li+ adducts instead of Na+ adducts. Preliminary data In source fragmentation of permethylated Lacto-N-difucoHexaose I and LS tetrasaccharide B was first studied in DHB. While the MS/MS of the Na+ adducts of these compounds (performed by LID) produces intenses B and Y fragments, those resulting from in source fragmentation are mainly oxonium ions, resulting from the cleavage of a glycosylic bond without any exchange of hydrogen atoms. These oxonium fragments were also obtained for lithium adducts. It was previously described that these fragments are produced by the cleavage of a protonated glycosidic bond. These ions carry their positive charge on a trivalent oxygen atom and are therefore not present on the spectra as sodium adducts. Since the peaks of protonated glycans are very low in MALDI spectra, it would indicate that protonation of glycosidic bonds of permethylated glycans would strongly favor a fragmentation reaction. Different matrices were tested to compare their ability to induce in source fragmentation of permethylated glycans. Interestingly, ATT gave similar results comparing to DHB while HCCA showed a lesser ability to promote in source fragmentation. However, the most striking result came from the use of 9-AA. This matrix, which is usually used in negative ion mode, was able to produce easily sodium adducts ions of permethylated glycan with a satisfying signal to noise ratio in positive ion mode. Moreover, practically no in source fragmentation was observed with this matrix. The few produced fragments were B ions but no oxonium ions were detected. Presence of these B fragments was increased for Li+ adducts. As 9-AA is the most basic of tested matrices, the absence of oxonium ions could result from its inability to transfer protons to the glycosidic bond of permethylated glycans. 9-AA could therefore become a matrix of choice to study complex mixtures of glycans, by reducing artefact peaks produced by ISD. Novel aspect ISD of permethylated glycans is induced by DHB while 9-AA strongly favors the presence of molecular ions. [less ▲]

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See detailOvergenerality Bias and these Consequences in Borderline Personality Disorder.
Boulanger, Marie ULg; Smets, Virginie; Blairy, Sylvie ULg

Poster (2010, June)

Objective: The present study investigated the Autobiographical Memory (AM) in borderline disorder population. AM is an entity that encompasses the individuals’past personnal experiences. Previous ... [more ▼]

Objective: The present study investigated the Autobiographical Memory (AM) in borderline disorder population. AM is an entity that encompasses the individuals’past personnal experiences. Previous researches have shown disturbances in AM among several psychiatric disorders such as depression, schizophrenia and bipolar disorder. This disturbances take the overgeneral retrieving form. Thus, when patients were asked to retrieve a specific event located in time and place, they recalled an overgeneral event. This deficit is not an isolated phenomen. Indeed the researches showed that AM deficits is related to decreasing of the ability to solve interpersonnal problem (Evans et al., 1992; Goddard et al., 1996) and impairments to project onself into the specific future events (D’argembeau et al., 2008; Williams et al., 1996). Impairments to respond adequately to social problems or to concrete plans for the future create hopelessness and to contribute to suicide attempt (Arie et al., 2008). Given the high risk of suicide or suicide attempts present in the Bordeline Personnality Disorder (BPD), consideration of AM in this population is appropriate. The aim of the present study was investigate the AM, the projection into the future and the problem solving in patients suffering from BPD. Method: 21 subjects BPD and 21 healthy controls participated in this study. First, the participants were asked to complete TeMA (validated French versions of AMT by Neumann & Philippot, 2006). Participants were instructed to generate specific past and future memories in response to cues words. Secondly, they were had to complete the OTT, they were asked to yield the most solutions as possible to daily problems. Finally the depression was controlled as well as neuropsychological variables. Results: The subjects with BPD recalled less specific past events and imagined less specific future events than healthy subjects (t(40) = 2.21, p = .031; t(40) = 3.4, p = .001, respectively). In addition, the number of past and future specific events was marginally correlated (r(42) = .31, p = .051). However, no difference between two groups on OTT and no correlation between past specificity and problem solving emerged. Discussion: As other clinical populations, the subjects with a BPD encounter deficits to retrieve specific past events. Moreover, these impairments are associated with deficits to imagine specific future events. Nevertheless, the ability to generate specific events was not related to the ability to solve problem. The observation of reduced specificity in the generation of autobiographical material is particularly clinically relevant. Indeed, difficulty in imagining the future may contribute to relapse. In conclusion, more systematic measure of this ability should be taken in both research and clinical fields. [less ▲]

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See detailKraft RB : classification of relevant variables with Random Forests
Sainlez, Matthieu ULg

Poster (2010, June)

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See detailThe Venus OH Nightglow Distribution
Soret, Lauriane ULg; Gérard, Jean-Claude ULg; Piccioni, Giuseppe et al

Poster (2010, June)

The first identification of the OH airglow in the terrestrial mesosphere was made in 1950 by Meinel [1950]. Recently, the unexpected presence of the OH nightglow was observed in the Venus mesosphere by ... [more ▼]

The first identification of the OH airglow in the terrestrial mesosphere was made in 1950 by Meinel [1950]. Recently, the unexpected presence of the OH nightglow was observed in the Venus mesosphere by Piccioni et al. [2008] using a limb profile from the Visible and Infra-Red Thermal Imaging Spectrometer (VIRTIS) instrument on board the Venus Express spacecraft. They clearly identified the (1-0) and (2-1) transitions at 2.80 and 2.94 µm, respectively and the (2-0) band at 1.43 µm. Additional bands belonging to the Δv=1 sequence also appear to be present longward of the (1-0) band. In a preliminary study of characteristics of the OH emission distribution, Gérard et al. [2010] pointed out a correlation between the OH(Δv=1) and the O2(a1Δ) nightglow intensities. In Soret et al. [2010], the full dataset of VIRTIS-M limb observations of the OH Venus nightglow has been corrected from the thermal emission of the planet and analyzed to determine its characteristics. Based on 3328 limb profiles, the study shows that the emission is highly variable. No clear dependence of the airglow layer altitude versus the antisolar angle is established. The peak brightness appears to decrease away from the antisolar point even if the variability at a given location is very strong. Some correlation between simultaneous observations of the intensity of the OH and the O2(a1∆) emissions has also been detected, presumably because atomic oxygen is a common precursor to the formation of O2(a1∆) and O3, whose reaction with H produces excited OH. A relation given in the one-dimensional photochemical model of Krasnopolsky [2009] has been used to link the OH and the O2(a1∆) airglows through the hydrogen flux at 130 km. It appeared that using a constant flux did not fill well the simultaneous OH and O2 observations. Either the flux has to vary with the distance to the antisolar point or other dimensions have to be involved. [less ▲]

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See detailNREM slow wave oscillation rebound after sleep deprivation : effect of aging
Lafortune, M; Viens, I; Poirier, G et al

Poster (2010, June)

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See detailVHHs as model proteins to investigate amyloid fibril formation: Fibril stability and effect of seeding and cross-seeding on aggregation kinectics
Chavignon, Chloé ULg

Poster (2010, June)

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidosis are known so far, amongst ... [more ▼]

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidosis are known so far, amongst which Alzheimer's disease, type II diabetes and immunoglobulin amyloidosis [1]. Although the mechanism of amyloid fibrils formation at the molecular level is not yet completely understood, it has been shown that the capacity to form amyloid fibrils in vitro is an intrinsic property of all polypeptide chains [1]. The choice of model proteins to investigate the aggregation process in vitro is therefore no more restrained to proteins involved in amyloidosis but can be settled on a wide variety of proteins. In this study, we have chosen to investigate the mechanism of amyloid fibrils formation by two variable domains of camelid heavy-chain antibodies (referred to as VHHs or nanobodies), cAb-HuL6 and cAb-BcII10, and this choice was motivated by the following reasons: - First, VHHs are small monomeric proteins (~14 kDa) presenting a high stability and a high solubility [2], which permits their expression with a high yield (20-40 mg.L-1). - Second, a wide range of stable mutants of these two VHHs is available. Mutations located at the disulfide bond [3,4], the CDRs [3] and the framework have been introduced. Characterisation of the aggregating properties of these mutants will allow the investigation of the impact of these structural elements on the process of fibril formation. In order to determine conditions in which cAb-HuL6 and cAb-BcII10 are more susceptible to form intermediates and thus amyloid fibrils, heat-induced unfolding experiments at pHs comprised in a range from 2,5 to 9,5 have been monitored by intrinsic fluorescence, ANS binding and circular dichroism. Then, aggregation experiments have been performed in the selected conditions and the presence of amyloid fibrils has been acknowledged by thioflavineT fluorescence experiments and electronic microscopy. We will discuss the kinetics of aggregation obtained in the absence and the presence of seeding/cross-seeding. [1] Chiti and Dobson, Annu. Rev. Biochem., 75, 2006, 333-366. [2] Dumoulin et al., Protein Sci., 11, 2002, 500-515. [3] Saerens et al., J. Mol. Biol., 352, 2005, 597-607. [4] Saerens et al., J. Mol. Biol., 377, 2008, 478-488. [less ▲]

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See detailA ‘cook book’ method for dioxin & PCBs analysis
Focant, Jean-François ULg; Shirkhan; Patterson Jr

Poster (2010, June)

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See detailImpact of morphosyntactic complexity in sentence comprehension in children with SLI
Leclercq, Anne-Lise ULg; Majerus, Steve ULg; Jacob, Laura et al

Poster (2010, June)

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See detailpCO2 dynamics and related airice CO2 fluxes during sea-ice growth and decay in an ice-tank experiment
Delille, Bruno ULg; Thomas, D. N.; Dieckmann, Gerhard et al

Poster (2010, June)

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See detailBrain-computer interface in disorders of consciousness: answering simple questions with a P3 speller
Noirhomme, Quentin ULg; Chatelle, Camille ULg; Kleih, Sonja et al

Poster (2010, June)

Objective: In the recovery from coma, the acquisition of command following represents an important milestone, indicating emergence from the vegetative state (Schnakers et al., 2009). In some patients ... [more ▼]

Objective: In the recovery from coma, the acquisition of command following represents an important milestone, indicating emergence from the vegetative state (Schnakers et al., 2009). In some patients, recovery of consciousness may precede motor recovery. Brain-computer interfaces (BCI) might permit these patients to show non-motor dependent signs of awareness and in a next step might enable communication. This study aimed at testing to what extent an EEG-based BCI could help detecting signs of awareness and communication in disorders of consciousness. We employed a P300 based BCI where healthy volunteers and patients with locked-in syndrome and in a minimally conscious state were asked to answer yes or no to simple questions by paying attention to one out of four auditorily presented stimuli (‘yes’, ‘no’, ‘stop’, ‘go’). Methods: We studied 13 patients with a minimally conscious state (MCS, 5 TBI – 8 anoxic, mean time post injury 70±109 months; mean age 42 ± 21) and 2 in pseudo-coma or locked in syndrome (LIS; brainstem stroke, time post injury 26 and 46 months; aged 63 and 29)) and 16 healthy controls (aged 45±19). Patients were evaluated using the Coma Recovery Scale Revised (CRS-R). An auditory P300 four choice speller paradigm (Furdea et al., 2009) based on the BCI2000 system (Schalk et al., 2004) was used. 16-Channel EEG was recorded using a g.tec USBAmp amplifier. A trial constituted of 15 presentation of four sounds the order of presentation being pseudo-randomized (sound duration: ~400ms; inter-stimulus interval: ~600ms). After a training session of 4 trials, patients and healthy subjects were required to answer 10 or 12 questions, respectively. Questions were of the following kind: “Is your name Quentin?”, “Is your mother’s name Dorothée?”. A stepwise linear discriminant analysis based on the training session was used to classify the data and to provide online feedback. Offline, all training and testing sequences were pooled. Sequences with artifacts were discarded and a leave-one-out approach was used to classify the data. Results: Healthy subjects presented a mean correct response rate of 73% online and 93% offline. Note that online classification failed for one control subject due to a presumed change in cognitive strategy between training and testing sessions. LIS patients showed a correct response rate of 30 and 60% (online) and 36 and 79% (offline). Three MCS patients had a correct response rate of ≥50% offline (10, 18, 0% online and 50, 53, 57% offline). Two of these three patients did not show any command following at the bedside. The 10 remaining MCS cases showed online and offline correct answers <50% (mean 33±9% online and 25±13% offline). Conclusion: Our auditory P300-based BCI permitted functional interactive communication in 15/16 controls (online) and in all offline. Our data obtained in patients with locked-in syndrome and disorders of consciousness demonstrate the potential clinical usefulness of the technique following coma but also show lower accuracy in patients as compared to healthy volunteers. This might be due to fluctuating attentional levels and exhaustibility in the MCS and to the suboptimal EEG recording quality due to movement, ocular and respiration artifacts in these challenging patients. Further algorithmic developments should include automatic artifact detection and single trial classification. Despite the need for further improvement in BCI devices adapted to post-coma patients, our results already indicate that MCS patients without any clinical sign of command-following can employ a yes-no speller offering the hope of functional interactive communication and a possibility for decision making and autonomy. Bibliography Furdea A, Halder S, Krusienski DJ, Bross D, Nijboer F, Birbaumer N, Kübler A, 2009, An auditory oddball (P300) spelling system for brain-computer interfaces, Psychophysiology. May; 46(3):617-25. Schalk G., McFarland D.J., Hinterberger T., Birbaumer N., and Wolpaw J.R. 2004, BCI2000: A General-Purpose Brain-Computer Interface (BCI) System, IEEE Trans Biomed Eng, 51(6). Schnakers C, Vanhaudenhuyse A, Giacino J, Ventura; Boly M, Majerus S, Moonen G, Laureys S, 2009, Diagnostic accuracy of the vegetative and minimally conscious state: Clinical consensus versus standardized neurobehavioral assessment, BMC Neurology, 9 (35). [less ▲]

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See detailClick chemistry : radiolabelling of oligonucleotides with fluorine- 18 for PET
Kaisin, Geoffroy ULg; Flagothier, Jessica ULg; Mercier, Frédéric ULg et al

Poster (2010, June)

Oligonucleotides (ONs), especially small interfering RNA (siRNA), are promising therapeutic agents, but their pharmacokinetics and biodistributions are widely unknown. Positron Emission Tomography (PET ... [more ▼]

Oligonucleotides (ONs), especially small interfering RNA (siRNA), are promising therapeutic agents, but their pharmacokinetics and biodistributions are widely unknown. Positron Emission Tomography (PET) using fluorine-18 is a suitable technique to image and quantify such biological processes. The challenge for the radiochemist is to introduce this short half-life isotope (t1/2(18F)=109.7 min) onto oligonucleotides or, more generally, biomolecules. The most common technique requires the coupling of a prosthetic group bearing the radiotracer with the biomolecule. Current methods for labeling ONs with fluorine-18 have sub-optimal yields and require a long synthesis time.1 Click chemistry, e.g. 1,3-dipolar Huisgen cycloaddition of azides to alkynes, could be an efficient way to increase yields and reduce synthesis time. Conjugations with ONs are usually performed at 3’-ends using a well-chosen linker in order to limit degradation by exonucleases and to avoid alteration of hybridization properties and siRNA gene silencing efficiency. This also allows the development of universal solid supports used for the solidphase synthesis of ONs. Here we report the synthesis of three alkyne-bearing linkers , the synthesis and radiosynthesis of the complementary azido-bearing prosthetic groups (1-(azidomethyl)-4-[18F]- fluorobenzene) and coupling with functionalized ONs. [less ▲]

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See detailAutomated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors
Goblet, David ULg; Thonon, David ULg; Plenevaux, Alain ULg et al

Poster (2010, May 30)

TOPIC: Molecular Neuroimaging: from Bench to Bedside Automated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors Introduction: Dysfunction of the cerebral serotoninergic system is ... [more ▼]

TOPIC: Molecular Neuroimaging: from Bench to Bedside Automated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors Introduction: Dysfunction of the cerebral serotoninergic system is implicated in numerous neurodegenerative disorders such as Alzheimer disease’s, dementia, depression, anxiety, schizophrenia, and Parkinson disease’s. The 5-HT1A serotonin receptors are involved in several physiological functions including sleep, mood, neurogenesis and learning [1]. Consequently, there have been huge efforts in finding ligands for this receptor. [11C]WAY-100635 is a high affinity radioligand used for quantifying serotonin 5-HT1A receptors with positron emission tomography. An 18F-labeled radioligand is advantageous because of higher specific activity and physical/nuclear properties (t1/2= 109 min, 97% of positron decay and positron energy of 635 keV maximum). [18F]MPPF, a selective 5-HT1A antagonist derived from WAY-100635, is currently one of the most successful PET ligand used for 5-HT1A receptor imaging [2]. However the affinity is lower then WAY-100635 and the amount of [18F]MPPF reaching the brain is relatively low since MPPF is a substrate for p-glycoprotein [3]. Methods: In order to improve the brain uptake of the radiotracer, a desmethylated analog has been developed in our lab and preliminary in vitro studies show positive results [4]. Nevertheless, the radiosynthesis take place in two steps as a protecting group removal is needed. A one step procedure with a MPPF derivative could be of very great interest. We have synthesized many MPPF derivatives in our lab (modification on the phenylpiperazine moiety) and developed an automated radiosynthesis procedure for the production of these radiotracers. [18F]MPPF was chosen as the model compound. We used a GE Healthcare FASTlabTM module and made modifications to the [18F]FDG synthesis sequence and cassette. [18F]MPPF was synthesized by coupling of [18F]FBA with the corresponding amine. After coupling, the crude solution was diluted with water and passed through a tC18 cartridge for prepurification. After elution, the [18F]MPPF was purified by semi-preparative HPLC. Results: Total synthesis time, including purification was approximately 100 min. [18F]FBA and [18F]MPPF were obtained at a corrected yield of 55% (n=20) and 25% (n=5) respectively. The radiochemical purity, checked by radio-TLC and UPLC, was >95%. Conclusions: We have developed an automated method for [18F]MPPF and derivatives production using a commercial synthesizer (FASTlabTM from GE Healthcare) and a conventional HPLC system resulting in good yields and high (radio)chemical purity. By simply switching the vial containing the modified amine, an 18F-labeled MPPF derivative could be obtained. Radiosynthesis is still under optimization and the radiotracers synthesized need to be tested as suitable 5-HT1A radioligands. Acknowledgement: This work was supported by the Fondation Rahier. References: [1] Filip M., Bader M. et Al, Pharmacol Rep. 2009 Sep-Oct; 61(5):761-77 [2] Aznavour N, Zimmer L. Et Al, Neuropharmacology. 2007 Mar; 52(3):695-707 [3] Laćan G., Plenevaux A. et Al, Eur J Nucl Med Mol Imaging. 2008 Dec;35(12):2256-66 [4] Defraiteur C., Plenevaux A. et Al., Br J Pharmacol. 2007 Nov; 152(6):952-8 [less ▲]

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See detailIs Anosognosia in Alzheimer disease also observed for behavioural and personality changes?
Feyers, Dorothée ULg; Bastin, Christine ULg; Jaspar, Mathieu ULg et al

Poster (2010, May 28)

Anosognosia is a frequent manifestation in Alzheimer disease (AD) but its extent is not yet clearly established. While anosognosia for memory deficit has been widely reported, no study has simultaneously ... [more ▼]

Anosognosia is a frequent manifestation in Alzheimer disease (AD) but its extent is not yet clearly established. While anosognosia for memory deficit has been widely reported, no study has simultaneously explored anosognosia for personality and behaviour changes. We have tackled this question with 20 AD patients and 20 matched elderly subjects (ES). Participants (AD and ES) assessed their personality and their reactions in social situation both in current (S1) and past (S1_bef) time period. Assessment of these characteristics was also performed by relatives of the participants (R2 and R2_bef). Mann-Whitney test (p<0.05) were performed between discrepancy scores (calculated by comparing answers of subjects and relatives) obtained for AD and ES. A specific measure of anosognosia was also calculated by comparing S1 and R2. Statistical analyses demonstrated (1) that relatives of AD patients report more personality and behavioural changes across time (S1-S1_bef) than relatives of ES (R2–R2_bef); (2) that self-reported changes were not significantly different between AD patients and ES; (3) that anosognosia (S1-R2) was observed in AD patients for personality changes only. Results obtained support the hypothesis that anosognosia does not affect all domain in AD. Indeed, even if AD patients are no more able to assess their current personality, they perceive adequately their current reactions in social situations. [less ▲]

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See detailUsing phenomenology and mindset induction to assess the prospective function of mind-wandering
Stawarczyk, David ULg; Majerus, Steve ULg; D'Argembeau, Arnaud ULg

Poster (2010, May 28)

A notable feature of the human cognitive apparatus resides in its propensity to spontaneously generate thoughts uncoupled from the “here and now”. An important function of these cognitions, often referred ... [more ▼]

A notable feature of the human cognitive apparatus resides in its propensity to spontaneously generate thoughts uncoupled from the “here and now”. An important function of these cognitions, often referred to as mind-wandering, might be to create and/or update scripts, schemata, and future plans in long-term memory. In this study, we investigated this hypothesis by examining whether priming personal projects influenced the occurrence and characteristics of mind-wandering episodes during a subsequent, unrelated cognitive task, as assessed with an experience sampling method. We found that inducing particular mindsets that were related to personal goals (i.e., writing an essay about one’s personal projects) in comparison to a control baseline condition (i.e., writing an essay about a familiar itinerary) increased the number of future-oriented mind-wandering reports while participants performed the Sustained Attention to Response Task (SART). Furthermore, participants judged most of these thoughts as having a future-oriented function (i.e., they were related to planning, decision making, or reevaluating situations). Finally, as behavioral validation of participants’ subjective reports, we found that mind-wandering was positively linked with intra-individual variability (IIV) in response times, whereas reports of being concentrated on the SART were negatively linked with IIV. These data support the view that an important function of mind-wandering resides in the anticipation and planning of the future. [less ▲]

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See detailFeeling of doing in compulsive checking
Belayachi, Sanaa ULg; Van der Linden, Martial ULg

Poster (2010, May 28)

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See detailConsciousness of memory functioning in Alzheimer’s disease
Jaspar, Mathieu ULg; Feyers, Dorothée ULg; Bastin, Christine ULg et al

Poster (2010, May 28)

Metamemory is a multi-faceted concept which deals with the individual’s knowledge and control of memory functioning. Previous studies that have examined the ability of Alzheimer’s disease (AD) patients to ... [more ▼]

Metamemory is a multi-faceted concept which deals with the individual’s knowledge and control of memory functioning. Previous studies that have examined the ability of Alzheimer’s disease (AD) patients to monitor efficiently their memory processes provided contradictory results. These discrepancies between studies could be the result of two factors: the kind of memory task used (episodic, semantic) and the kind of memory process on which memory monitoring is assessed (encoding, maintenance, retrieval). In the present study, different aspects of memory monitoring in 21 AD patients and 21 healthy elderly participants were explored with two tasks : a semantic memory task assessing the feeling-of-knowing (FOK) accuracy for general knowledge and an episodic memory task assessing judgment-of-learning (JOL) and FOK accuracy for information associated to a specific spatiotemporal encoding context By comparison to healthy participants, AD patients exhibit impaired performance on episodic FOK accuracy but not on semantic FOK accuracy. Moreover, no difference was observed between the two groups on the JOL post-encoding accuracy. These results confirm that not all aspects of memory monitoring are impaired in AD. Indeed, although there exists an impairment of episodic FOK performance, semantic FOK and JOL post-encoding appear preserved. The dissociation between the two FOK performance could be due to recruitment of more automatic processes for metacognitive judgment on general knowledge (semantic FOK) than for metacognitive judgment based on specific recent experience (episodic FOK). Similarly, a global prediction during maintenance (JOL) could be based on more automatic processes than an item-by-item judgment during retrieval. [less ▲]

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See detailWhat is the impact of the sequence structure on implicit learning in children?
Lejeune, Caroline ULg; Schmitz, Xavier ULg; Lempereur, Stéphanie et al

Poster (2010, May 28)

It is generally admitted that implicit learning abilities are efficient early in childhood. However, few studies have explored the impact of the structure of the sequence on children’s performance in ... [more ▼]

It is generally admitted that implicit learning abilities are efficient early in childhood. However, few studies have explored the impact of the structure of the sequence on children’s performance in implicit learning tasks. The current research was intended to examine sequence learning abilities in children by comparing sequences of different structural characteristics. [less ▲]

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See detailThe links between dyslexia and inhibition deficits: do dyslexic children with an inhibition deficit have a specific reading profile?
Verhaegen, Clémence ULg; Schelstraete, Marie-Anne; Noël, Marie-Pascale

Poster (2010, May 28)

The present study focused on the nature of the reading disability of children with both dyslexia and inhibition deficit. Van der Schoot et al. (2000; 2004); Nossent et al. (2005); Nossent and Deroux (2006 ... [more ▼]

The present study focused on the nature of the reading disability of children with both dyslexia and inhibition deficit. Van der Schoot et al. (2000; 2004); Nossent et al. (2005); Nossent and Deroux (2006) showed that these children tended more to guess the words they read because these “guessers” found difficulties in suppressing false candidate words that are activated in the lexicon. To test this account, dyslexic children with and without inhibition deficit were presented a sentence reading task. To evoke guessing, each sentence contained a word or a pseudoword which closely resembled either a valid congruent or incongruent word. Our hypothesis was that dyslexic children with inhibition deficit guessed more the word they read from the sentence context and the global word form than dyslexic children without inhibition deficit. The results showed that both dyslexics with and without inhibition deficit tended more to guess the words than control children without dyslexia and inhibition difficulties. We discussed these results with the fact that most of children with reading difficulties use the sentence context to compensate for poor reading skills. [less ▲]

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See detail18F labelling of Insulin via click chemistry
Paris, Jérôme ULg; Mercier, Frédéric ULg; Thonon, David ULg et al

Poster (2010, May 27)

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See detailImprovement of Physicians’ Patient-Centeredness: An Empirical Evidence of the Benefits of a Communication Skills Training Program
Jonius, Bénédicte ULg

Poster (2010, May 27)

Objectives. No study has yet assessed the benefits of a communication skills training program on patient-centeredness. The purpose of this study is to assess the association between ... [more ▼]

Objectives. No study has yet assessed the benefits of a communication skills training program on patient-centeredness. The purpose of this study is to assess the association between patients’characteristics and physicians’ communication skills before and after a communication skills training program. Methods. Correlations between physicians’ communication skills and patients’ characteristics were categorized as absent or present: the number of present correlations indicates the level of patientcenteredness. Communication skills were assessed during a consultation before and after a communication skills training program. Consultations were audio-recorded and transcripted. Communication skills were rated according to the Cancer Research Campaign Workshop Evaluation Manual. Patients’ characteristics (sociodemographic, physical and psychological) were measured before and after the training program. Results. Sixty-three physicians were included. Before training, physicians’ communication skills were associated with one patients’ sociodemographic characteristic and with one psychological characteristic. After training, physicians’ communication skills were associated with none of the patients’ sociodemographic characteristics and with four psychological characteristics. Conclusions. This study highlights that physicians’ patient-centeredness may be improved through a communication skills training program. After training, physicians are more centered on patients’ psychological characteristics and not on patients’ sociodemographic characteristics anymore. This study underlines the need to design communication skills training which may further improve patient-centeredness. [less ▲]

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See detailMethylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach
Dupont, Aurélie ULg; Bouquegneau, Jean-Marie ULg; Das, Krishna ULg et al

Poster (2010, May 26)

Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood ... [more ▼]

Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood cells, including the immune cells, are therefore exposed to the toxic properties of that chemical. Nevertheless, selenium (Se) is an essential element absorbed concomitantly to MeHg which seems to modulate this toxicity. The goal of this study is to evaluate the immunotoxicity of MeHg on the harbor seal (Phoca vitulina) T lymphocytes, highly important in the adaptive immune response, and to investigate the modulating effect of Se on that toxicity. In parallel, the concentrations of MeHg, total mercury (T-Hg) and Se are determined in free-ranging harbour seal blood in order to follow their contamination levels. The T lymphocytes were isolated from the whole blood, exposed to various MeHg and Se concentrations and the exposure effects were estimated by functional tests including the evaluation of viability, proliferation, metabolic activity, DNA and protein synthesis, and by morphological analysis by transmission electron microscopy. The mean T-Hg concentration was 172 ± 143 µg/l of whole blood. The T lymphocytes cultures in vitro displayed a decreasing number of viable cells with increasing concentrations of MeHg, and numerous ultrastructural defects. The cells exposed to MeHg notably displayed distortion of the plasmic membrane, nucleus fragmentations, chromatin compaction, swelling mitochondrias and cytoplasmic vacuolisations. Those results highlighted various immunotoxic effects of MeHg, both at the functional and ultrastructural levels. The antagonistic role of Se on MeHg immunotoxicity is discussed. [less ▲]

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See detailTailor-made polymers by cobalt-mediated radical polymerization
Debuigne, Antoine ULg; Hurtgen, Marie ULg; De Winter, Julien et al

Poster (2010, May 25)

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See detailStimuli-responsive triblock copolymer for biomedical applications
Sibret, Pierre ULg; Croisier, Florence ULg; Zhao, J. et al

Poster (2010, May 25)

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See detailLe rôle du vagabondage de l’esprit dans le maintien d’un état anxiété
Stawarczyk, David ULg; Blanchy, Sarah ULg; D'Argembeau, Arnaud ULg

Poster (2010, May 25)

Le vagabondage de l’esprit (c’est-à-dire, l’apparition spontanée de pensées prenant leur origine dans la mémoire à long-terme et sans lien avec la tâche en cours de réalisation) est un phénomène fréquent ... [more ▼]

Le vagabondage de l’esprit (c’est-à-dire, l’apparition spontanée de pensées prenant leur origine dans la mémoire à long-terme et sans lien avec la tâche en cours de réalisation) est un phénomène fréquent qui représente en moyenne 10 à 30% de nos pensées journalières. Les recherches dans le domaine suggèrent que ce type de pensées est lié à la gestion de nos préoccupations actuelles et que sa fréquence peut être augmentée par l’induction d’affects négatifs. Cependant, le rôle possible de ces pensées dans le maintien des affects négatifs est encore mal connu. Dans cette étude, une préoccupation anxiogène (supposément devoir réaliser un discours filmé) ou une préoccupation neutre (supposément devoir réaliser une tâche cognitive de réflexion) a été induite immédiatement avant la réalisation d’une tâche exécutive d’inhibition durant laquelle la fréquence et le contenu des pensées distractives ont été évalués. Trois mesures de l’état émotionnel des participants ont été réalisées : (1) au début de la session, (2) après l’induction de la préoccupation et (3) après la tâche exécutive. Les résultats montrent la présence d’un plus grand nombre de pensées distractives liées à la préoccupation induite, d’un plus grand nombre d’erreurs d’inhibition ainsi que d’une augmentation significative des affects négatifs entre les temps 1 et 2 dans la condition anxiogène par rapport à la condition neutre. Par ailleurs, une corrélation négative entre le nombre de pensées distractives liées au discours et la diminution de l’anxiété entre les temps 2 et 3 est observée. Ces résultats démontrent l’importance des cognitions anticipatoires spontanées dans le maintient de l’anxiété. [less ▲]

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See detailEffects of androgenic and anti-androgenic substances on the freshwater gastropod Lymnaea stagnalis
Giusti, Arnaud ULg; Ducrot, Virginie; Joaquim-Justo, Célia ULg et al

Poster (2010, May 25)

Knowledge on the impacts of endocrine disrupting chemicals (EDCs) on gastropods is scarce and their mechanisms of action are still poorly understood. In this study, effects of 3 androgens (tributyltin ... [more ▼]

Knowledge on the impacts of endocrine disrupting chemicals (EDCs) on gastropods is scarce and their mechanisms of action are still poorly understood. In this study, effects of 3 androgens (tributyltin, testosterone and fenitrothion), 2 anti-androgens (cyproterone acetate and vinclozolin) and 1 estrogen (chlordecone) on growth and reproduction were investigated in the hermaphrodite gastropod Lymnaea stagnalis. [less ▲]

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See detailImpacts of androgenic and antiandrogenic substances on the freshwater pulmonate gastropod Lymnaea stagnalis
Giusti, Arnaud ULg; Lagadic, Laurent; Joaquim-Justo, Célia ULg et al

Poster (2010, May 23)

Knowledge on the impacts of endocrine disruptors on gastropods is scarce and their mechanism of action poorly understood especially the impacts of androgenic and antiandrogenic compounds. In this study ... [more ▼]

Knowledge on the impacts of endocrine disruptors on gastropods is scarce and their mechanism of action poorly understood especially the impacts of androgenic and antiandrogenic compounds. In this study effects of 5 androgenics and antiandrogenics endocrine disruptors were investigated on the reproduction and life traits of the freshwater pulmonate gastropod Lymnaea stagnalis. The chemicals tested were Tributyltin, Cyproterone acetate, Methyltestosterone, Vinclozolin and Fenitrothion. Tributyltin was used as androgenic positive control. The other compounds tested are androgenic and antiandrogenic compounds either steroids or non steroids. Adult snails were exposed to 3 concentrations of each chemical for 30 days. The size and reproductive organs development were monitored throughout exposure. The number of clutches, the number of eggs per clutch were assessed during the first 10 days. The clutches were kept individually in clear water except for the clutches of the eighth day which were divided in two. Half was kept individually in clear water and the other half was individually reared in contaminated water following the same exposure as their parents. The percentage of hatching per clutch of these clutches was measured. The development size, mortality, and development of reproductive organs of the juveniles were assessed until their first clutch was laid. [less ▲]

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Peer Reviewed
See detailRelationship between BET specific surface area, DR- and t-plot pore volume
Scherdel, C.; Reichenauer, G.; Wiener, M. et al

Poster (2010, May 23)

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See detailImpact of anti-androgens on the production of spermatozoa in Brachionus calyciflorus
Joaquim-Justo, Célia ULg; Cruciani, Valentina ULg; BLASZCZYK, Marie ULg et al

Poster (2010, May 23)

Putative endocrine disruptors affect rate of fertilization of sexual females by males. Preston et al. (2000) reported a decrease in fertilization rate following exposition to, notably, flutamide at 10µg/l ... [more ▼]

Putative endocrine disruptors affect rate of fertilization of sexual females by males. Preston et al. (2000) reported a decrease in fertilization rate following exposition to, notably, flutamide at 10µg/l. We observed similar effects with fenitrothion. Cross mating experiments showed that the decrease in fertilization rate is due to an impact on males exclusively. We studied the impact of anti-androgens on spermatogenesis and observed a decrease in the spermatozoa production with concentrations down to 0,5 mg L-1.We also report a detailed description of the morphology of the male reproductive apparatus in B. calyciflorus based on optic and electronic microscopy analysis and we describe the effect of anti-androgens on males and spermatogenesis in males. [less ▲]

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See detailLes enzymes et leur utilisation en biotechnologie industrielle
Galonde, Nadine ULg

Poster (2010, May 22)

Detailed reference viewed: 38 (9 ULg)
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See detailmiR-146a: an anti-angiogenic miRNA regulated by NF-kB
Halkein, Julie ULg; Tabruyn, Sébastien ULg; Malvaux, Ludovic et al

Poster (2010, May 21)

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See detailComplementation of Saccharomyces cerevisiae mutants using a soil metatranscriptomic library
Kellner, Harald ULg; Luis, Patricia; Portetelle, Daniel ULg et al

Poster (2010, May 21)

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See detailInvolvement of miR-125b in in vitro and in vivo angiogenesis
Malvaux, Ludovic; Pendeville, Hélène; Sabatel, Céline et al

Poster (2010, May 21)

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See detail16K human prolactin is an anti-lymphangiogenic factor in vitro and in vivo
Kinet, Virginie; Castermans, Karolien; Blacher, Silvia ULg et al

Poster (2010, May 21)

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See detailEtude du mode de liaison des canaux potassiques de type SK et l’apamine par modélisation moléculaire
Dilly, Sébastien ULg; Lamy, Cédric; Seutin, Vincent ULg et al

Poster (2010, May 20)

Parmi les canaux ioniques impliqués dans le contrôle de l'activité neuronale, les canaux potassiques calcium-dépendants de basse conductance, dénommés canaux SK, constituent une cible thérapeutique ... [more ▼]

Parmi les canaux ioniques impliqués dans le contrôle de l'activité neuronale, les canaux potassiques calcium-dépendants de basse conductance, dénommés canaux SK, constituent une cible thérapeutique intéressante. En effet, ils sous-tendent la posthyperpolarisation ("AfterHyperPolarization") de durée moyenne (mAHP) qui limite l'excitabilité de divers types de neurones du système nerveux central (SNC). A ce jour, 3 types de sous-unités, SK1, SK2 et SK3, ont été identifiés dans différentes régions du cerveau. Le blocage sélectif de ces canaux pourrait être bénéfique dans le traitement de divers troubles du SNC comme la maladie de Parkinson (SK3), la dépression (SK3) ou encore les désordres cognitifs (SK2) (Liégeois et al., 2003 ; Pedarzani et al., 2008). Jusqu’à présent, le site d’interaction entre les canaux SK et leurs bloqueurs n’a pas encore été précisément élucidé. Dans ce contexte, nous avons entrepris la modélisation de ces canaux par homologie comparative en se basant sur la structure cristalline du canal potassique KCSA (Doyle et al., 1998). La construction de ces canaux constitue la première étape dans la détermination des requis structuraux essentiels à l’affinité de bloqueurs et à la compréhension des modes de liaison de ces ligands. Le mode de liaison de l’apamine, bloqueur peptidique issu du venin d’abeille, a été ensuite exploré par « docking ». Afin de confirmer ce site de liaison potentiel, des expériences de mutagénèse dirigée ont été réalisées. Les premiers canaux mutants testés dans des expériences électrophysiologiques par la technique de « patch clamp » ont permis de valider certaines données théoriques. Grâce à cette stratégie, nous espérons préciser le mécanisme d'action des bloqueurs des canaux SK et, idéalement, découvrir des pistes pour concevoir des bloqueurs sélectifs. [less ▲]

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See detailDe la prévention chronologique à la prévention relationnelle; la prévention quaternaire comme tâche du médecin de famille.
Jamoulle, Marc ULg

Poster (2010, May 19)

La prévention clinique est traditionnellement exprimée comme une vue du médecin sur une tâche à réaliser avant la survenue d’un évènement. Initiée par Leavell et Clark en 1958 elle est complétée en 1988 ... [more ▼]

La prévention clinique est traditionnellement exprimée comme une vue du médecin sur une tâche à réaliser avant la survenue d’un évènement. Initiée par Leavell et Clark en 1958 elle est complétée en 1988 par J-A Bury qui dénomme prévention quaternaire les soins palliatifs.Le paradigme change, le patient devient central aux soins, la prévention clinique est perçue au travers de la relation entre le médecin et le patient. Le croisement de la science du médecin et de la conscience du patient permet de répartir la prévention en quatre champs. Elle est traversée en oblique par la ligne du temps. Cette proposition (Jamoulle, 1986) a été avalisée par la Wonca et l’UEMO et permet de cadrer quatre concepts de prévention clinique. [less ▲]

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See detailBiological control using Episyrphus balteatus (De Geer) (Diptera: Syrphidae)
Leroy, Pascal ULg; Verheggen, François ULg; Capella, Quentin et al

Poster (2010, May 18)

Augmentative biocontrol constitutes a safe way to reduce pest populations through the enhancement of natural enemies’ activity. In this context, the aphidophagous syrphid Episyrphus baltetaus (De Geer ... [more ▼]

Augmentative biocontrol constitutes a safe way to reduce pest populations through the enhancement of natural enemies’ activity. In this context, the aphidophagous syrphid Episyrphus baltetaus (De Geer) (Diptera: Syrphidae) is a promising candidate for aphid biological control: larvae of this syrphid attack and consume a wide range of aphid species and are found on many vegetable crops. Because natural populations of beneficials are not always sufficient to regulate the pest infestations, this work has focused on the conception of a biological control device containing syrphid eggs which can easily be introduced in fields or greenhouses. Using semiochemicals [E-(β)-farnesene, R-(+)-limonene and (Z)-3-hexenol], honeydews and “artificial honeydews” (10% or 30% aqueous solutions of sucrose, fructose and glucose), the syrphid oviposition was artificially induced on an inert surface. To test and validate the biological control device described above, laboratory and field experiments were performed: a plastic lamella covered with syrphid eggs was suspended on aphid infested plants in order to measure the efficiency of the device. The results obtained were promising since populations of 500 aphids were eliminated in ten days when 15 syrphid eggs were introduced. The use of such a biological control device could certainly contribute to aphid biological control strategies. [less ▲]

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See detailA Far-UV Imager for the Jupiter Ganymede Orbiter
Bunce, E. J.; Molyneux, P.; Bannister, N. et al

Poster (2010, May 17)

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See detailAvifaune forestière - Etude d'habitat du Pic mar
Lehaire, François ULg; Paquet, Jean-Yves; Delahaye, Laurence et al

Poster (2010, May 12)

En tant que bio-indicateurs, les espèces spécialistes des grands massifs forestiers feuillus ont un intérêt pour l'étude et le suivi de la diversité de ces milieux.

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See detailTypologie des chênaies de la Région wallonne
Perin, Jérôme ULg; Brostaux, Yves ULg; Claessens, Hugues ULg

Poster (2010, May 12)

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See detailL'inventaire du bois mort à terre
Ligot, Gauthier ULg; Rondeux, Jacques ULg

Poster (2010, May 12)

Le bois mort à terre est devenu un indicateur reconnu de la biodiversité et de la gestion durable des forêts. Il importe donc de pouvoir apprécier les quantités de mort à l'hectare. Choisir la méthode d ... [more ▼]

Le bois mort à terre est devenu un indicateur reconnu de la biodiversité et de la gestion durable des forêts. Il importe donc de pouvoir apprécier les quantités de mort à l'hectare. Choisir la méthode d'échantillonnage à utiliser reste une tâche ardue bien qu'il existe une abondante littérature sur le sujet. Ce poster compare deux techniques généralement utilisées : les placettes de taille fixe et les transects. Nous avons évalué ces deux méthodes aussi bien à l'échelle locale que nationale et au travers de simulations statistiques nous en avons évalué la précision et l'efficacité. De plus, nous mettons en évidence l'importance d'utiliser des fonctions de conversion des estimations obtenues dans un but d'harmonisation internationale des résultats. [less ▲]

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See detailL'inventaire forestier national permanent du Grand-Duché de Luxembourg, aide au monitoring de la biodiversité
Alderweireld, Matthieu ULg; Kugener, Georges; Wagner, Marc et al

Poster (2010, May 12)

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See detailL'inventaire forestier national permanent du Grand-Duché de Luxembourg, aperçu de la méthodologie
Alderweireld, Matthieu ULg; Kugener, Georges; Wagner, Marc et al

Poster (2010, May 12)

L'inventaire forestier national du Grand-Duché de Luxembourg assure depuis 1998 la mesure par échantillonnage des 89 000 ha de forêt que compte le pays. La collecte des données s'étale sur 3 ans et est ... [more ▼]

L'inventaire forestier national du Grand-Duché de Luxembourg assure depuis 1998 la mesure par échantillonnage des 89 000 ha de forêt que compte le pays. La collecte des données s'étale sur 3 ans et est réalisée tous les 10 ans au niveau d'unités d'échantillonnage à placettes concentriques. Ces données concernent tous les aspects de la forêt dans l'esprit de multifonctionnalité qui lui est assigné. [less ▲]

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See detailSTEM CELL IN THE TREATMENT OF TENDONITIS IN EQUINE PATIENTS
Verwilghen, Denis ULg; Briquet, Alexandra ULg; Gothot, André ULg et al

Poster (2010, May 07)

Detailed reference viewed: 118 (23 ULg)