References of "Poster"
     in
Bookmark and Share    
Full Text
See detailDIVA: a Data Analysis Software with Generalized-Cross Validation and Quality Control
Troupin, Charles ULg; Rixen, Michel; Sirjacobs, Damien ULg et al

Poster (2007, May 19)

Detailed reference viewed: 16 (1 ULg)
Full Text
See detailMitochondrion plasticity evidenced by coupled comparisons of mitochondrial and cellular proteomes
Mathy, Grégory ULg

Poster (2007, May 16)

Mitochondria are key organelles in primary metabolism: they are the main source of energy production and the starting point of important biosynthetic pathways. Thus the control of the mitochondrial ... [more ▼]

Mitochondria are key organelles in primary metabolism: they are the main source of energy production and the starting point of important biosynthetic pathways. Thus the control of the mitochondrial function is a prerequisite for cellular survival in front of a wide spectrum of exogenous or endogenous stresses. By using the SILAC method, we have investigated the yeast mitochondrial and cellular proteome adaptation to the heterologous expression of a mitochondrial alternative oxidase (AOX). AOX is an energy dissipating enzyme that catalyses the re-oxidation of ubiquinol within the respiratory chain and compete with the Cytochrome pathway for electrons and thus, prevents proton pumping, and consequently leads to a decrease in ATP synthesis. [less ▲]

Detailed reference viewed: 17 (4 ULg)
Full Text
Peer Reviewed
See detailModels of irrigation system for Pursat basin planning
Ly, Sarann ULg; Someth, Paradis; Men, Nareth

Poster (2007, May 14)

The Mekong River and the Tonle Sap Great Lake in Cambodia have vast floodplain suitable for rice-based farming system. The Tonle Sap Basin, defined as the catchments of the Tonle Sap River at its ... [more ▼]

The Mekong River and the Tonle Sap Great Lake in Cambodia have vast floodplain suitable for rice-based farming system. The Tonle Sap Basin, defined as the catchments of the Tonle Sap River at its confluence with the Mekong River and other 11 main tributaries draining higher ground on all sides of the basin enclosing 44% of Cambodia’s land area. The Pursat River is one of tributaries of the Tonle Sap Great Lake. It originates at Cardamom Mountains and has very good potential for agricultural development. In this paper, we aim at applying three models of irrigation system for basin planning: Chinit (Dam-Reservoir Irrigation), West Baray (Reservoir Irrigation) and Batheay (Shallow Reservoir Irrigation) to Pursat Basin (one of sub-basin of the Tonle Sap Great Lake). Chinit Dam-Reservoir stores water supplied by one of tributaries of Tonle Sap Great Lake, the Chinit River, located in Kampong Thom Province. Spillway is built to pass up inundation and to release water to downstream part. The rest of water in the Dam-Reservoir is distributed to the paddy field by main canal, secondary canals and tertiary canals. This system can be put at the upstream part of the Pursat River, mountainous area. The West Baray Irrigation system is characterized by a reservoir combined with a dike system in the floodplain of the Tonle Sap Great Lake and an irrigation system situated in Siem Reap province. The reservoir is supplied by a tributary of the Tonle Sap Great Lake, the Siem Reap River, in rainy season. The water is distributed to irrigation area in dry season. A small dike system was constructed in the Tonle Sap floodplain for retarding and storing floodwater. The stored floodwater is used as an additional water source for dry season cultivation. This system can be placed at the central plain of the Pursat Basin. Batheay reservoir directly receives floodwater from the Mekong River. It functions as both a reservoir and a paddy field. In wet season, dike around the Batheay reservoir prevents floodwater from entering the reservoir. Rainy season rice is grown inside the reservoir. After harvesting, gates around the reservoir are opened to receive floodwater. The water is stored for cultivating dry season rice outside the reservoir. This system can be put at the downstream part of the Pursat River, floodplain area of Tonle Sap River. [less ▲]

Detailed reference viewed: 41 (4 ULg)
See detailSimulation of annual cycles of phytoplankton, zooplankton and nutrients to the South of Gran Canaria
Troupin, Charles ULg; Sangrà, Pablo; Arístegui, Javier

Poster (2007, May 11)

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailLe bm-573, un antagoniste original de récepteur au thromboxane a2, réduit le développement des lesions atheromateuses chez des souris deficientes en apolipoproteine e (apo e-/-)
Cherdon, Céline ULg; Rolin, Stéphanie; Hanson, Julien ULg et al

Poster (2007, May 10)

Afin d’examiner l'efficacité du BM-573 dans l'athérogenèse, des souris apo E-/- ont été traitées durant 10 semaines avec le BM573 (10mg/kg). Au cours de cette expérience, le traitement des animaux par le ... [more ▼]

Afin d’examiner l'efficacité du BM-573 dans l'athérogenèse, des souris apo E-/- ont été traitées durant 10 semaines avec le BM573 (10mg/kg). Au cours de cette expérience, le traitement des animaux par le BM-573, a eu pour effet de diminuer les lésions athéromateuses précoces de manière significative. Ces données ont été confirmées par des analyses histopathologiques et biochimiques. Ces résultats confirment que l'antagonisme sélectif des récepteurs TP associé à une inhibition de la thromboxane synthase réduit significativement les lésions athéromateuses chez les souris apoE-/-.. Le BM-573 est, par conséquent, un agent thérapeutique potentiel pour la prévention de l'athérosclérose [less ▲]

Detailed reference viewed: 30 (7 ULg)
Peer Reviewed
See detailCharacterization of gels via solvent desorption measurements
Gommes, Cédric ULg; Noville, Francis; Pirard, Jean-Paul ULg

Poster (2007, May)

Detailed reference viewed: 2 (0 ULg)
Peer Reviewed
See detailExploring the risk factors for Salmonella in the ten biggest Belgian pig slaughterhouses.
Delhalle, Laurent ULg; Desadeleer, L.; Bollaerts, K. et al

Poster (2007, May)

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailGlobal strategy for the development of estrogenic compounds detection screening test
Collodoro, Mike ULg; Makasinga, Elu; Lemaire, Pascale ULg et al

Poster (2007, May)

Detailed reference viewed: 15 (1 ULg)
See detailResolution recovery iterative reconstruction for human pinhole SPECT: a phantom study.
Seret, Alain ULg; Defrise, Michel; Andreyev, André et al

Poster (2007, May)

Detailed reference viewed: 30 (1 ULg)
See detailDaily quality control of activimeters: an absolute usefulness.
Seret, Alain ULg; Greffe, J.-L.

Poster (2007, May)

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailConception et synthèse d’outils pharmacologiques originaux pour l’étude des récepteurs au thromboxane
Moray, A. L.; Dogne, J. M.; Neven, P. et al

Poster (2007, May)

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailValorisation in bread of dietary fibres from by-products of the agro-industries.
Roiseux, Olivier; Sindic, Marianne ULg; Vanderbeke, E. et al

Poster (2007, May)

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailThe 1979-2005 Greenland ice sheet melt extent from passive microwave data using an improved version of the melt retrieval XPGR algorithm
Fettweis, Xavier ULg

Poster (2007, April 16)

Analysis of passive microwave satellite observations over the Greenland ice sheet reveals a significant increase in surface melt over the period 1979-2005. Since 1979, the total melt area was found to ... [more ▼]

Analysis of passive microwave satellite observations over the Greenland ice sheet reveals a significant increase in surface melt over the period 1979-2005. Since 1979, the total melt area was found to have increased +1.22 x 10ˆ7 kmˆ2. An improved version of the cross-polarized gradient ratio (XPGR) technique is used to identify the melt from the brightness temperatures. The improvements in the melt retrieval XPGR algorithm as well as the surface melt acceleration are discussed with results from a coupled atmosphere-snow regional climate model. From 1979 to 2005, the ablation period increases everywhere over the melt zone except in the regions where the model simulates an increased summer snowfall. Indeed, more snowfall in summer decreases the liquid water content of the snowpack, raises the albedo and therefore reduces the melt. Finally, this melt acceleration over the Greenland ice sheet is highly correlated with both Greenland and global warming suggesting a continuing surface melt increase in the future. [less ▲]

Detailed reference viewed: 17 (0 ULg)
See detailMorphology of the Io footprint
Bonfond, Bertrand ULg; Gérard, Jean-Claude ULg; Grodent, Denis ULg

Poster (2007, April 15)

Detailed reference viewed: 4 (0 ULg)
Full Text
See detailCalcification and transparent exopolymer particles (TEP) production in batch cultures of Emiliania huxleyi exposed to different pCO2
De Bodt, Caroline; d'Hoop, Quentin; Harlay, Jérôme ULg et al

Poster (2007, April 15)

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP ... [more ▼]

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP accumulate until the end of the experiment and are enhanced after nutrient exhaustion. TEP contribute significantly to POC concentrations after the exponential growth phase. The very good correlation between TEP and calcite concentrations suggests that the calcification acts as a potential source of TEP in coccolithophore blooms. Finally, if calcite continues to decrease, calcification may be hampered in this species. [less ▲]

Detailed reference viewed: 18 (0 ULg)
See detailSCAR-MarBIN, the Antarctic marine biodiversity information network.
Danis, Bruno; et al.; Dauby, Patrick ULg et al

Poster (2007, April 12)

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailNK CELLS AND NKG2D LIGANDS IN HPV-ASSOCIATED CERVICAL CANCER
Jacobs, Nathalie ULg; Renoux, Virginie ULg; Dortu, Estelle ULg et al

Poster (2007, April 12)

Detailed reference viewed: 31 (3 ULg)
Full Text
Peer Reviewed
See detailAminoglycoside antibiotics and the sensory hair cells of inner ear and lateral line system in the Atlantic cod, Gadus morhua: effects on fish hearing threshold
Faucher, Karine ULg; Aas-Hansen, Øyvind; Damsgard, Borge et al

Poster (2007, April)

The aims of the present study were to investigate: a) the potential involvement of the fish lateral line system in hearing at 250 Hz and b) the possible regeneration of the inner ear hair cells in the ... [more ▼]

The aims of the present study were to investigate: a) the potential involvement of the fish lateral line system in hearing at 250 Hz and b) the possible regeneration of the inner ear hair cells in the Atlantic cod (Gadus morhua). The inner ear and lateral line system of the Atlantic cod were inactivated using ototoxic aminoglycoside antibiotics by injection (gentamicin) or bath (gentamicin and streptomycin), respectively. Hearing thresholds were measured in the fish using the Auditory Brainstem Response (ABR) technique at 10, respectively 17 - 20 days following antibiotic treatment. Results were compared between groups of individuals i) with inner ear damage, ii) with lateral line system altered, iii) placebo (immersion in fresh sea water) and iv) control. The sound stimuli applied were series of 2000 five-cycle (2-1-2) 250 Hz tone bursts delivered using a Brüel & Kjær (Nærum, Denmark) loudspeaker. ABRs were measured using a filter set at 30-3000 Hz, a gain of 100 000 and visual interpretation using the Bio-Logic AEP software. Sound stimulus level was reduced until threshold was attained. To check the tissue status of the inner ear and lateral line system, hair cells from sensory maculae were visualized using scanning electron microscope (SEM). Ten days after treatment, hearing thresholds of placebo and lateral line damaged cods did not differ from controls fish (p = 0.387 and p = 0.095, respectively), whereas the fish that received intravenous gentamicin injection had significantly elevated hearing thresholds compared to controls (p < 0.0001). SEM analyses further demonstrated that, 10 days following antibiotic treatment, cod inner ear hair cells were damaged, as well as lateral line hair cells and more particularly in the case of canal neuromasts. There was no hearing effect or microscopic tissue damage in untreated fish. From the 17th day after treatment, ABR measurements showed that some of the gentamicin treated fish had regained hearing thresholds comparable to the control cods (p = 0.535), and this was associated with an apparent regeneration of hair cells in the sensory maculae of their inner ear. This study indicates a capacity for inner ear hair cell regeneration in Atlantic cod, and illustrates the advantages of the ABR-method for investigation of fish hearing after intervention on the inner ear, and during the hair cell regeneration process. The present work further suggests that the lateral line system is not involved in cod hearing at 250 Hz. [less ▲]

Detailed reference viewed: 65 (6 ULg)
See detailInter-annual variability of the carbon dioxide oceanic sink south of Tasmania
Borges, Alberto ULg; Tilbrook, B.; Metzl, N. et al

Poster (2007, April)

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailEvaluation of a new commercial real time PCR for the detection of Aspergillus spp. in serum and respiratory samples
Hayette, Marie-Pierre ULg; Meex, Cécile ULg; Boreux, Raphaël ULg et al

Poster (2007, April)

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ ... [more ▼]

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ widely and comparisons are difficult to assess. The objective of the study is to compare a new commercial real-time PCR kit, affigene® Aspergillus tracer assay, with an in house nested PCR targeting 18S rRNA Aspergillus sp. gene. Methods. Twelve patients at risk for invasive aspergillosis were included in the study. They were classified to have possible (5 cases), probable (1 case) or proven (6 cases) invasive aspergillosis following E.O.R.T.C. criteria. Fifteen serum and respiratory paired samples were collected. The DNA extraction was performed by using the QIAmp DNA mini kit® (Qiagen, Germany). All samples were tested by both PCR assays and respiratory samples were cultured. Results. Respiratory samples. A. fumigatus, A. niger and A. flavus were isolated from 10/15 samples; both PCR methods were positive for these samples except one that was positive for affigene® and equivocal for the nested PCR. The real-time PCR assay reported cycle thresholds ranging from 25 to 38. Three of the five culture-negative samples were negative by both PCR methods; one of three was negative in affigene® assay and equivocal by nested PCR; the last sample was positive in affigene® assay and negative by nested PCR. Serum. Thirteen of fifteen blood samples were negative by both PCR methods. One sample was equivocal by nested PCR and was inhibited in affigene® assay despite a culture-positive paired respiratory sample. The last case was inhibited by the real-time PCR assay and negative by nested PCR. Nor the nested PCR, nor affigene® assay could detect any Aspergillus DNA in serum. In total, there was 93% of agreement between the two PCR assays. Conclusion. Both methods are in good agreement and can detect at least three different species of Aspergillus. However, the sensitivity of both assays does not permit the detection of Aspergillus DNA in serum. affigene® assay can easy replace the “in house” assay: it allows a fast and standardized detection of Aspergillus sp. DNA in respiratory samples without inconvenient due to the handling of PCR products. [less ▲]

Detailed reference viewed: 81 (5 ULg)
Full Text
See detailDissolved inorganic carbon dynamics in the Gulf of Biscay (June 2006)
Suykens, K.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007, April)

Detailed reference viewed: 3 (1 ULg)
See detail. Study of Neuronal Preconditioning by ESR
Guelluy, Pierre-Henri ULg; Hoebeke, Maryse ULg

Poster (2007, April)

Detailed reference viewed: 6 (1 ULg)
Peer Reviewed
See detailInfluence of collar design on palatal implant primary stability
Bolland, Fabrice; Ronco, Vincent; LAMBERT, France ULg et al

Poster (2007, April)

Detailed reference viewed: 51 (0 ULg)
See detailDatation par luminescence des terres cuites architecturales : étude de briques de l’abbaye de St Philbert de Grandlieu (44, France)
Blain, Sophie ULg; Guibert, Pierre; Vieillevigne, Emmanuelle et al

Poster (2007, April)

Detailed reference viewed: 6 (0 ULg)
Full Text
See detailDetermination of COF2 vertical distributions above Jungfraujoch by FTIR and multi-spectra fitting
Duchatelet, Pierre ULg; Mahieu, Emmanuel ULg; Demoulin, Philippe ULg et al

Poster (2007, April)

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. Photolysis of these compounds leads to release of chlorine atoms, while the fluorine is, in a first step, present in the form of ... [more ▼]

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. Photolysis of these compounds leads to release of chlorine atoms, while the fluorine is, in a first step, present in the form of carbonyl compounds like COClF and COF2. Their further photolysis liberates fluorine atoms, which are quickly converted to HF. Given its long life time, COF2 is the second stratospheric fluorine reservoir. The first COF2 vertical distributions were derived from occultation measurements performed by the ATMOS instrument during the SPACELAB-3 Space Shuttle mission in 1985. The Canadian FTIR spectrometer ACE-FTS, onboard the SCISAT-1 satellite, is the first instrument since the last ATMOS flight in 1994, to record COF2 vertical profiles from space. All these observations show that, at mean latitudes, COF2 concentration is maximum between 30 and 35 km. Several COF2 IR absorption lines located either in the so-called InSb (1-5 µm) and MCT (2-16 µm) spectral ranges can be used to determine its total column from ground-based FTIR observations. In this context, several studies concerning the evolution of COF2 total column above various stations were published during the nineties. At this time, no study concerning the inversion of COF2 vertical distributions from ground-based FTIR spectra has been published. This report deals with the feasibility of such inversions, using, simultaneously, via the SFIT-2 v3.91 algorithm, a multi-microwindows and a multi-spectra fitting procedure. The multi-spectra method consists of combining several FTIR observations, recorded during the same day, to increase the information content. A selection of microwindows in InSb and MCT ranges, a complete discussion about the data characterization (e.g. information content) and typical examples of COF2 retrieved profiles from high resolution solar spectra recorded with the University of Liège Jungfraujoch FTS will be revealed. [less ▲]

Detailed reference viewed: 49 (7 ULg)
Full Text
See detailApproaches for retrieving abundances of methane isotopologues in the frame of the AGACC project from ground-based FTIR observations performed at the Jungfraujoch
Duchatelet, Pierre ULg; Mahieu, Emmanuel ULg; Demoulin, Philippe ULg et al

Poster (2007, April)

This work has been performed within the frame of AGACC(http://www.oma.be/AGACC/Home.html), a contribution to the Belgian Scientific Support for a Sustainable Development. The project intends to make an ... [more ▼]

This work has been performed within the frame of AGACC(http://www.oma.be/AGACC/Home.html), a contribution to the Belgian Scientific Support for a Sustainable Development. The project intends to make an advanced exploitation of existing ground-based remote-sensing measurements for a selection of atmospheric species that play an important role in the chemistry of the atmosphere and that have a direct or indirect impact on climate. Target species include -among others- lower tropospheric aerosols, H2O, HDO, CH4, HCN and CO. The instrumentation includes 3 types of spectrometers (FTIR, MAXDOAS and Brewer) and one CIMEL sun photometer. These instruments are operated at 3 different sites (Jungfraujoch, Ile de la Réunion and Uccle) and most of them are affiliated with the Network for the Detection of Atmospheric Composition Change (NDACC, formerly NDSC - http://www.ndacc.org), a group dedicated to performing high-quality long-term observations. This contribution will deal with the detection of the isotopologues of methane, a species released to the atmosphere by natural processes (e.g. wetlands, termites) as well as by anthropogenic activities (e.g. fossil fuel exploitation, rice agriculture, biomass burning, etc). Due to its high warming potential and its relatively long chemical lifetime, atmospheric methane is a major greenhouse gas. Methane also affects climate by influencing tropospheric ozone and stratospheric water. The cycle of methane is complex and a thoroughly study of the sources and sinks of its main isotopologue, as well as the other isotopic species, is necessary to characterize it. Isotopic ratios are also useful to differentiate between various sources of atmospheric methane. To study the vertical distribution of methane isotopologues from the high resolution FTIR spectra recorded by the University of Liège instrument at the International Scientific Station of the Jungfraujoch (ISSJ; 46.5°N, 8.0°E, 3580m a.s.l., Swiss Alps), we have selected several 13CH4 lines distributed in the so-called InSb (1-5 µm) and MCT (2-16 µm) spectral ranges. A set of four microwindows has also been selected for the study of CH3D. Using the SFIT-2 v3.91 algorithm, vertical column abundances as well as low-resolution vertical distributions have been retrieved, adjusted from an a priori profile defined on a 41 layers scheme and derived from ACE-FTS space observations. The information content and first preliminary retrieval results will be presented. [less ▲]

Detailed reference viewed: 131 (1 ULg)
Full Text
See detailMeasured and modelled trends of stratopsheric Cly and Fy column amounts in the northern hemisphere
Ruhnke, Roland; Blumenstock, Thomas; Duchatelet, Pierre ULg et al

Poster (2007, April)

Reactive inorganic chlorine plays a crucial role in the stratospheric ozone depletion. To stabilize and enable a recovering of the stratospheric ozone layer, the Montreal protocol and its amendments and ... [more ▼]

Reactive inorganic chlorine plays a crucial role in the stratospheric ozone depletion. To stabilize and enable a recovering of the stratospheric ozone layer, the Montreal protocol and its amendments and adjustments have been progressively implemented to reduce or even stop the production and emission of important chlorinated source gases (CFCs, HCFCs, CCl4, CH3CCl3, and Halons). As these source gases are photolysed in the stratosphere into inorganic chlorine and fluorine, respectively, the turn over of the inorganic chlorine (HCl and ClONO2) and slowing down of fluorine (HF and COF2) reservoirs act as a verification of the effectiveness of these protocols. Here we present results of long-term measurements of the stratospheric column of HCl, ClONO2, and HF obtained at different stations in the northern hemisphere (Ny Alesund, Kiruna, Zugspitze, Jungfraujoch, Izana, all affiliated to the NDACC, Network for the Detection of Atmospheric Composition Change) within the PEP (Pole- Equator-Pole) network. These time series are interpreted with model calculations performed with a state of the art 2-D model and the 3-D CTM KASIMA with respect to the determination of the slowing down or turn over, respectively. In addition, trend parameters calculated using different approaches (e.g. linear trend, bootstrap-method) will be presented and intercompared. [less ▲]

Detailed reference viewed: 30 (5 ULg)
Peer Reviewed
See detailProduction de pseudoparticules de norovirus humains et bovins et leur utilisation diagnostique
Mauroy, Axel ULg; Scipioni, Alexandra; Saegerman, Claude et al

Poster (2007, April)

Les norovirus sont des agents majeurs de gastroentérite humaine d’origine alimentaire et ceci à travers le monde entier. La contamination est habituellement oro-fécale. Les norovirus sont non enveloppés ... [more ▼]

Les norovirus sont des agents majeurs de gastroentérite humaine d’origine alimentaire et ceci à travers le monde entier. La contamination est habituellement oro-fécale. Les norovirus sont non enveloppés et ont un génome composé d’ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Au sein de ce génome, trois cadres ouverts de lecture (ORFs) sont décrits. L’ORF 1 code pour une polyprotéine qui sera par la suite clivée pour donner les différentes protéines non-structurales. L’ORF 2 encode pour l’unique protéine de capside. Une protéine structurale mineure dont le rôle est encore peu caractérisé est encodée par l’ORF 3. Ces virus sont très résistants dans l’environnement et une infection peut survenir même avec une très faible dose infectieuse. Appartenant à la famille des Caliciviridae, le genre norovirus est composé de cinq génogroupes (G) et contient également des virus infectant les animaux (bovins, porcins, murins). L’impact des norovirus animaux et plus particulièrement des norovirus bovins peut être envisagé à travers un possible caractère zoonotique de ces virus ; ainsi ils pourraient par exemple être transmis à l’homme via des eaux d’effluent contaminées. L’étude des norovirus humains et bovins est encore à l’heure actuelle entravée par l’absence d’un système de culture cellulaire. Cependant différents systèmes d’expression protéique ont été utilisés afin d’exprimer la protéine de capside, celle-ci s’assemblant spontanément avec d’autres pour reformer des pseudoparticules virales ou virus-like particles (VLPs). Ces VLPs sont morphologiquement et antigéniquement semblables aux virus natifs. Le but de ce travail était dans un premier temps d’obtenir des VLPs de souches humaines et bovines. Les gènes codant pour la protéine de capside d’une souche humaine (H384) et d’une souche bovine (B309) ont été amplifiés par RT-PCR à partir d’échantillons de matières fécales collectés dans des laboratoires de diagnostic humain et vétérinaire. Ces gènes ont été séquencés et comparés grâce à des outils informatiques (Basic Local Alignment Search Tool, BLAST) à la banque de séquences déjà disponibles sur le site du NCBI. Nous avons pu déterminer que la séquence de capside de la souche H384 était proche de celle de la souche HuNV/Altenkirchen 140/01/DE, une souche de GII et de génotype 4 tandis que la séquence de la souche B309 était proche de celles de GIII et de génotpe 2 (groupe Newbury2). Des plasmides contenant les séquences des ORFs 2 de deux souches de norovirus humains de référence, la souche de Norwalk (GI) et celle de Hawaii (GII), ont été aimablement fournies par le Docteur Jan Vinjé (CDC, Atlanta, USA). Le système d’expression protéique baculovirus (Invitrogen) a été utilisé pour obtenir des VLPs. Brièvement, des cellules d’insecte des lignées Sf9 et H5 ont été infectées avec des baculovirus recombinants pour la protéine de capside des norovirus. Les VLPs ont été purifiées à partir des surnageants par ultracentrifugation sur un coussin de sucrose à 30% suivie d’une ultracentrifugation en gradient de chlorure de césium. Ce gradient a été fractionné et les différentes fractions ont été analysées pour la présence de la protéine de capside des norovirus par SDS-PAGE. Pour vérifier que les fractions positives contenaient bien des VLPs, les surnageants ont été observés en microscopie électronique. Nous avons ainsi obtenu des VLPs de norovirus de trois des cinq génogroupes décrits (GI, II et III). Ces VLPs ont été utilisées pour immuniser des souris afin de produire des anticorps monoclonaux et pour immuniser des lapins afin d’obtenir des anticorps polyclonaux. Nous avons pu aussi tester une banque de sérums bovins par ELISA pour la présence d’anticorps dirigés contre les souches de norovirus de génogroupe III et de génotype 2, les plus fréquemment mises en évidence au sein de notre banque de matières fécales bovines. [less ▲]

Detailed reference viewed: 16 (1 ULg)
Peer Reviewed
See detailEvaluation of the cytotoxicity of cyclodextrins on endothelial cells using MTT assay
Castagne, Delphine ULg; Belhadj Salem, Leila; Delattre, Luc ULg et al

Poster (2007, April)

Detailed reference viewed: 24 (1 ULg)
Full Text
See detailOverview on the ADONIS Project: Accurate Diagnosis of prostate cancer using Optoacoustic detection of biologically functionalized gold Nanoparticles - A new Integrated Biosensor System
Fleron, Maximilien ULg; Schol, Daureen ULg; Greisch, Jean-François et al

Poster (2007, March 21)

Overview on the ADONIS Project: Accurate Diagnosis of prostate cancer using Optoacoustic detection of biologically functionalized gold Nanoparticles - A new Integrated Biosensor System

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailPeculiar hydrophobic properties of the 67-78 fragment of α-synuclein are responsible for membrane destabilization and neurotoxicity
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Dupiereux-Fettweis, Ingrid ULg et al

Poster (2007, March 14)

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ... [more ▼]

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ago, tilted peptides have been detected in two other amyloidogenic proteins : the amyloid β peptide involved in Alzheimer’s disease, and the PrP protein linked to Creuztfeldt-Jakob’s disease. Tilted peptides are short protein fragments that adopt an oblique orientation when inserted into biological membranes. Tilted peptides are able to destabilize membranes. In this study, we predicted by sequence analysis and molecular modelling that the 67-78 fragment of α-synuclein is a tilted peptide. Like most of them, the α-syn 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. A mutant designed by molecular modelling to decrease the destabilizing properties of the peptide was shown to be significantly less fusogenic. The neuronal toxicity was studied using human neuroblastoma cells and we demonstrated that the α-syn 67-78 peptide induces neurotoxicity. In conclusion, we have identified a tilted peptide in α-synuclein which could be involved in the toxicity induced during amyloidogenesis of α-synuclein. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailSynthesis of functional amphiphilic copolymers for the elaboration of third generation drug nanocarriers
Alaimo, David ULg; Freichels, Hélène; Jérôme, Robert ULg et al

Poster (2007, March 08)

Detailed reference viewed: 13 (0 ULg)
Full Text
See detailCoccolithophorid calcium carbonate dissolution in surface waters
Harlay, Jérôme ULg; Koch, Craig; Young, Jeremy R et al

Poster (2007, March 06)

The role of calcifying organisms in the ocean biogeochemistry has been receiving increasing attention since CO2-related global change issues such as ocean acidification were pointed out by the scientific ... [more ▼]

The role of calcifying organisms in the ocean biogeochemistry has been receiving increasing attention since CO2-related global change issues such as ocean acidification were pointed out by the scientific community. The implications of changing oceanic pH in modifying ecosystems dominated by planktonic calcifiers have been shown by mesocosm and laboratory experiments based on CO2 manipulations. The major concern of such experiments focussed on variations in the rates of ecosystem primary production and calcification due to changes in algal physiology or specific composition. Our results, from an interdisciplinary survey of coccolithophore-dominated blooms in the northern Bay of Biscay (NE Atlantic), suggest that biogenic calcite dissolution is occurring in the photic zone where surface waters are oversaturated with respect to calcite. The dissolution of CaCO3 in surface waters, evidenced by scanning electron microscopy observations, has an impact on the preservation and export of carbon in coccolithophore-dominated ecosystems and on the exchange of CO2 across the ocean-atmosphere interface. Both aspects of suspended calcite concentration reduction in natural environments (lower rates of production or dissolution) could be considered as a perturbation of the oceanic carbon cycle. We aim at presenting here a biogeochemical description of processes, including integrated primary production, calcification, and parameters such as transparent exopolymer particles concentration and particulate inorganic carbon profiles, during field studies. A mechanism for calcite dissolution, based on biological activity in microenvironments (including grazing, bacterial respiration and DMS production) is presented as a conceptual model in coccolithophore blooms. [less ▲]

Detailed reference viewed: 23 (1 ULg)
Full Text
See detailCalcification and transparent exopolymer particles (TEP) production in batch cultures of Emiliania huxleyi exposed to different pCO2
De Bodt, Caroline; d'Hoop, Quentin; Harlay, Jérôme ULg et al

Poster (2007, March 06)

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP ... [more ▼]

Ehux growth, calcification and related processes are sensitive to changes in initial pCO2. Our results show that the development of the Ehux cultures is delayed with increasing initial pCO2. TEP accumulate until the end of the experiment and are enhanced after nutrient exhaustion. TEP contribute significantly to POC concentrations after the exponential growth phase. The very good correlation between TEP and calcite concentrations suggests that the calcification acts as a potential source of TEP in coccolithophore blooms. Finally, an Ωcalcite of 3 seems to be necessary for Ehux to calcify. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
See detailUse of exergy to detect and measure perturbations affecting Posidonia oceanica (L.) Delile meadows: Characterization in the sedimentary compartment
Pete, Dorothée ULg; Bouquegneau, Jean-Marie ULg; Gobert, Sylvie ULg

Poster (2007, March)

Within the scope of the European Water Framework Directive, it seems important to characterize state and evolution of marine ecosystems in a global way. So, it is proposed to use both thermodynamic ... [more ▼]

Within the scope of the European Water Framework Directive, it seems important to characterize state and evolution of marine ecosystems in a global way. So, it is proposed to use both thermodynamic indicators called exergy (Ex) and specific exergy (Exsp) in Posidonia oceanica meadows. Aims of this project are to detect and measure perturbations owed to nutrients loading and mechanical spoiling in the Mediterranean coastal zone. It will be led in the Bay of Calvi, on the microbenthic loop (organic matter, bacteria, microphytobenthos and meiofauna) and will be divided in two parts: sampling in different zone of the meadow and in situ experiments. In the end of this study, it will be possible to give a diagnostic on the health of P. oceanica meadow and to determine how this health will evolve, thanks to the introduction of the exergy in a model. [less ▲]

Detailed reference viewed: 36 (10 ULg)
See detailCO2 dynamics and related air-ice-sea gas transfer in spring pack and land fast sea ice,
Delille, Bruno ULg; Schoemann, V.; Lannuzel, D. et al

Poster (2007, March)

Detailed reference viewed: 8 (0 ULg)
Full Text
See detailThe role of deformation in the bouncing droplet problem
Terwagne, Denis ULg; Gilet, Tristan ULg; Dorbolo, Stéphane ULg et al

Poster (2007, March)

Detailed reference viewed: 11 (0 ULg)
See detailA role for the transcriptional complex elongator in cell migration and spreading
Creppe, C.; Close, Pierre ULg; Cornez, I. et al

Poster (2007, March)

Detailed reference viewed: 14 (3 ULg)
Full Text
See detailCarbon dioxide in European coastal waters
Borges, Alberto ULg; Schiettecatte, L.-S.; Abril, G. et al

Poster (2007, March)

Detailed reference viewed: 3 (0 ULg)
See detailPolyubiquitination of the scaffold protein tank/I-traf: implications for the TLR-mediated signalling pathways
Gatot, J. S.; Gioia, R.; Chau, Tieu-Lan ULg et al

Poster (2007, March)

Detailed reference viewed: 10 (4 ULg)
See detailBiogeochemical cycle in a coccolithophorid bloom
Suykens, Kim; Delille, Bruno ULg; Borges, Alberto ULg et al

Poster (2007, March)

Detailed reference viewed: 9 (1 ULg)
Full Text
See detailCompiling polymorphic miRNA-target interactions: the Patrocles database.
Hiard, Samuel ULg; Tordoir, Xavier ULg; Coppieters, Wouter ULg et al

Poster (2007, February 15)

Using positional cloning, we have recently identified the mutation responsible for muscular phenotype of the Texel sheep. It is located in the 3’UTR of the GDF8 gene - a known developmental repressor of ... [more ▼]

Using positional cloning, we have recently identified the mutation responsible for muscular phenotype of the Texel sheep. It is located in the 3’UTR of the GDF8 gene - a known developmental repressor of muscle growth - and creates an illegitimate target site for miRNA expressed in the same tissue. This causes miRNA-mediated translation inhibition of mutant GDF8 transcripts which leads to muscle hypertrophy. We followed up on this finding by searching for common polymorphisms and mutations that affect either (i) RNAi silencing machinery components, (ii) miRNA precursors or (iii) target sites. These might likewise alter miRNA-target interaction and could be responsible for substantial differences in gene expression level. They have been compiled in a public database (“Patrocles”: www.patrocles.org), where they are classified in (i) DNA sequence polymorphisms (DSP) affecting the silencing machinery, (ii) DSP affecting miRNA structure or expression and (iii) DSP affecting miRNA target sites. DSP from the last category were organized in four classes: destroying a target site conserved between mammals (DC), destroying a non-conserved target site (DNC), creating a non-conserved target site (CNC), or shifting a target site (S). To aid in the identification of the most relevant DSP (such as those were a target site is created in an antitarget gene), we have quantified the level of coexpression for all miRNA-gene pairs. Analysis of the numbers of Patrocles-DSP as well as their allelic frequency distribution indicates that a substantial proportion of them undergo purifying selection. The signature of selection was most pronounced for the DC class but was significant for the DNC and CNC class as well, suggesting that a significant proportion of non-conserved targets is truly functional. The Patrocles database allowed for the selection of DSP that are likely to affect gene function and possibly disease susceptibility. The effect of these DSP is being studied both in vitro and in vivo. In conclusion, Patrocles-DSP could be widespread and underlie an appreciable amount of phenotypic variation, including common disease susceptibility. [less ▲]

Detailed reference viewed: 29 (2 ULg)
Full Text
See detailPREDetector : Prokaryotic Regulatory Element Detector
Hiard, Samuel ULg; Rigali, Sébastien ULg; Colson, Séverine ULg et al

Poster (2007, February 15)

Background: In the post-genomic area, in silico predictions of regulatory networks are considered as a powerful approach to decipher and understand biological pathways within prokaryotic cells. The ... [more ▼]

Background: In the post-genomic area, in silico predictions of regulatory networks are considered as a powerful approach to decipher and understand biological pathways within prokaryotic cells. The emergence of position weight matrices based programs has facilitated the access to this approach. However, a tool that automatically estimates the reliability of the predictions and would allow users to extend predictions in genomic regions generally regarded with no regulatory functions was still highly demanded. Result: Here, we introduce PREDetector, a tool developed for predicting regulons of DNA-binding proteins in prokaryotic genomes that (i) automatically predicts, scores and positions potential binding sites and their respective target genes, (ii) includes the downstream co-regulated genes, (iii) extends the predictions to coding sequences and terminator regions, (iv) saves private matrices and allows predictions in other genomes, and (v) provides an easy way to estimate the reliability of the predictions. Conclusion: We present, with PREDetector, an accurate prokaryotic regulon prediction tool that maximally answers biologists’ requests. PREDetector can be downloaded freely at http://www.montefiore.ulg.ac.be/~hiard/predetectorfr.html [less ▲]

Detailed reference viewed: 23 (0 ULg)
Peer Reviewed
See detailFirst-Principles Study of Thermal and Elastic Properties of Al2O3
Xu, Bin ULg; Dong, Jianjun

Poster (2007, February)

Detailed reference viewed: 20 (3 ULg)
Full Text
See detailThe role of deformation in the bouncing droplet problem
Terwagne, Denis ULg; Gilet, Tristan ULg; Dorbolo, Stéphane ULg et al

Poster (2007, February)

Detailed reference viewed: 6 (0 ULg)
Full Text
See detailProduire de la viande et du lait avec la Blanc-Bleu Belge de type mixte
Bastin, Catherine ULg; Mayeres, Patrick; Bertozzi, Carlo et al

Poster (2007, January 24)

Detailed reference viewed: 25 (6 ULg)
Peer Reviewed
See detailCleft Palate (CP): not only a diagnosis in foals
Van Galen, Gaby ULg; Delguste, Catherine ULg; Verwilghen, Denis ULg et al

Poster (2007, January)

Detailed reference viewed: 24 (8 ULg)
Full Text
Peer Reviewed
See detailPreconditioning of the tumor vasculature and tumor cells by intermittent hypoxia: implications for anti-cancer therapies
Martinive, Philippe ULg

Poster (2007, January)

Hypoxia is a common feature in tumors associated with an increased resistance of tumor cells to therapies. In addition to O(2) diffusion-limited hypoxia, another form of tumor hypoxia characterized by ... [more ▼]

Hypoxia is a common feature in tumors associated with an increased resistance of tumor cells to therapies. In addition to O(2) diffusion-limited hypoxia, another form of tumor hypoxia characterized by fluctuating changes in pO(2) within the disorganized tumor vascular network is described. Here, we postulated that this form of intermittent hypoxia promotes endothelial cell survival, thereby extending the concept of hypoxia-driven resistance to the tumor vasculature. We found that endothelial cell exposure to cycles of hypoxia reoxygenation not only rendered them resistant to proapoptotic stresses, including serum deprivation and radiotherapy, but also increased their capacity to migrate and organize in tubes. By contrast, prolonged hypoxia failed to exert protective effects and even seemed deleterious when combined with radiotherapy. The use of hypoxia-inducible factor-1alpha (HIF-1alpha)-targeting small interfering RNA led us to document that the accumulation of HIF-1alpha during intermittent hypoxia accounted for the higher resistance of endothelial cells. We also used an in vivo approach to enforce intermittent hypoxia in tumor-bearing mice and found that it was associated with less radiation-induced apoptosis within both the vascular and the tumor cell compartments (versus normoxia or prolonged hypoxia). Radioresistance was further ascertained by an increased rate of tumor regrowth in irradiated mice preexposed to intermittent hypoxia and confirmed in vitro using distinctly radiosensitive tumor cell lines. In conclusion, we have documented that intermittent hypoxia may condition endothelial cells and tumor cells in such a way that they are more resistant to apoptosis and more prone to participate in tumor progression. Our observations also underscore the potential of drugs targeting HIF-1alpha to resensitize the tumor vasculature to anticancer treatments. PMID: 17178869 [PubMed - indexed for MEDLINE] [less ▲]

Detailed reference viewed: 44 (14 ULg)
Peer Reviewed
See detailL'utilité de l'association aripiprazole-méthylphénidate en pédopsychiatrie, selon les diagnostics
Scholl, Jean-Marc ULg; Philippe, Paule ULg

Poster (2007, January)

Introduction : En pédopsychiatrie, les enfants présentent souvent des comorbidités. L'AD et/ou HD peut être associée à tous les diagnostics de la nosographie. Une diversité de diagnostics répond aux ... [more ▼]

Introduction : En pédopsychiatrie, les enfants présentent souvent des comorbidités. L'AD et/ou HD peut être associée à tous les diagnostics de la nosographie. Une diversité de diagnostics répond aux antipsychotiques atypiques et l'ADHD répond au méthylphénidate. Par contre, il n'existe aucune étude qui analyse l'effet simultané d'aripiprazole-méthylphénidate chez l'enfant. Objectifs : Établir le diagnostic des comorbidités chez les enfants et évaluer dans un suivi de cohorte : l'efficacité de l'association aripiprazole-méthylphénidate, les posologies et les effets secondaires. Méthode : Établissement des diagnostics des comorbidités chez 31 enfants, 22 garçons et 9 filles, de 5 à 12 ans (moy: 9,1 ans), suivis dans 2 Services de Santé Mentale. Les diagnostics sont établis selon: le DSM IV et les critères des Troubles de la Régulation des stimuli sensoriels de la « Classification des Diagnostics des Troubles de la Santé Mentale et du Développement de la Petite Enfance, DC:0-3R », 2005. Les posologies et effets secondaires sont évalués dans un suivi longitudinal de 8 mois en moy/enfant. Résultats : Comorbidités : ADHD (31) [AD (4), HD (2), mixte (25)], états paranoïdes (11), autres projections psychotiques (10), symptômes négatifs (10), bipolarité (25) [maniaque (10), mixte (13), dépressif (2)], colères en "on-off" (18), troubles de la régulation (17), autisme (2), somnambulisme (1). Aripiprazole-méthylphénidate est efficace dans l'ensemble de ces diagnostics. L'aripiprazole est efficace dans les Troubles de la Régulation ; ceci est une nouvelle indication thérapeutique. Posologie. Aripiprazole : 1,0 mg/10 kilos (min 0,8 et max 1,8 mg), 1 prise/j. Méthylphénidate : 7 mg/10 kilos/j (min 5 et max 10 mg). Arrêt de l'aripiprazole : 2 arrêts pour accroissement de l'agressivité, 1 arrêt pour augmentation de la sensibilité des perceptions avec peurs. Arrêt du méthylphénidate : 1 arrêt pour coliques abdominales. Arrêt du méthylphénidate et de l'aripiprazole : aripiprazole suite à une somnolence importante non dose dépendante et méthylphénidate suite à un accroissement de l'excitabilité non dose dépendante chez 1 enfant. Conclusion : L'association aripiprazole-méthylphénidate est très bien tolérée, avec un bénéfice additif lorsque l'enfant présente en comorbidité un AD et/ou HD (26). [less ▲]

Detailed reference viewed: 135 (1 ULg)
Peer Reviewed
See detailL’aripiprazole chez les enfants de 5 à 12 ans : étude clinique. Indications, intérêt, posologie, effets secondaires
Scholl, Jean-Marc ULg; Philippe., Paule ULg

Poster (2007, January)

Introduction : Les antipsychotiques atypiques ont de tout autres propriétés que les neuroleptiques. Leurs indications comprennent de nombreux diagnostics "non psychotiques". Ils sont largement utilisés ... [more ▼]

Introduction : Les antipsychotiques atypiques ont de tout autres propriétés que les neuroleptiques. Leurs indications comprennent de nombreux diagnostics "non psychotiques". Ils sont largement utilisés chez les enfants. L'aripiprazole est le dernier venu des antipsychotiques atypiques et il est le premier agoniste partiel dopaminergique et sérotoninergique. Il a des propriétés originales dont les effets sont peu connus chez les enfants. Objectifs : Évaluer l'efficacité de l'aripiprazole selon les diagnostics, les posologies utiles et les effets secondaires dans un suivi de cohorte. Méthode : Étude transversale descriptive de 37 enfants, 25 garçons et 12 filles, âgés de 5 à 12 ans (moy: 8 ans 7 mois), suivis dans 2 Services de Santé Mentale. Les diagnostics sont établis selon: 1. le DSM IV et 2. les critères des Troubles de la Régulation des stimuli sensoriels de la « Classification des Diagnostics des Troubles de la Santé Mentale et du Développement de la Petite Enfance, (DC:0-3R, 2005) ». Une attention particulière est portée au diagnostic des comorbidités. Les posologies et effets secondaires sont évalués dans un suivi longitudinal de 8 mois en moy. par enfant. Résultats : L'aripiprazole est efficace dans les : états paranoïdes (14), autres projections psychotiques (10), symptômes négatifs (12), la bipolarité (25), les colères en "on-off" (18), les troubles de la régulation (21), l'autisme (3), le somnambulisme (2). Posologie thérapeutique : 1,0 mg/10 kilos (min. 0,8 et max 1,8 mg), 1 prise/j. Effets secondaires : nausées entre le Ier et le 10e jour (6), somnolence importante (2), augmentation de la sensibilité des perceptions durant les 4 premières semaines de traitement (4), accroissement de l'agressivité avec sentiments de persécution (2), épisodes de palpitations avec tachycardie (2). Arrêt du traitement : 2 arrêts pour somnolence importante non dose-dépendante (une enfant a une immaturité neurologique et l'autre une leucomalacie périventriculaire avec épilepsie), 2 autres arrêts pour accroissement de l'agressivité, et un 5e arrêt suite à des palpitations et tachycardie (examens cardiologiques normaux). L'association aripiprazole-méthylphénidate est très bien tolérée et apporte un bénéfice additif lorsque l'enfant présente en comorbidité un AD et/ou HD (26). [less ▲]

Detailed reference viewed: 806 (4 ULg)
Full Text
Peer Reviewed
See detailMiroir interne déformant de l'image de soi : élaboration conceptuelle intégrant la clinique et les découvertes récentes des neurosciences
Scholl, Jean-Marc ULg; Philippe, Paule ULg

Poster (2007, January)

Neurosciences : Un réseau neuronal spécifiquement référé au "self" du sujet est mis en évidence en neuroimagerie (Ph Fossati). Il intervient pour se remémore des attributs référés au self. Ce réseau est ... [more ▼]

Neurosciences : Un réseau neuronal spécifiquement référé au "self" du sujet est mis en évidence en neuroimagerie (Ph Fossati). Il intervient pour se remémore des attributs référés au self. Ce réseau est différent de celui qui permet au sujet de donner des attributs à d'autres individus. Le réseau référé au self permet d'être en contact immédiat avec l'image et la connaissance de soi. De plus, le rappel mnésique des attributs positifs et négatifs référés au self met en jeu des réseaux neuronaux différents. Les attributs positifs référés au self activent la région préfrontale antéromédiane droite, les attributs négatifs référés au self la région préfrontale dorsolatérale droite. Le sujet sain active les deux réseaux de façon simultanée pour restituer une image de soi intégrée. Définition conceptuelle: Le "miroir interne" – distingué de l'objet qui lui est présenté – correspond¬ au réseau neuronal référé spécifiquement au self. Ce réseau accède à l'image de soi. S'il y a un dysfonctionnement du réseau référé au self, il restitue une image déformée de soi, et le "miroir interne" devient déformant. Le sujet associera son identité à cette image déformée. Clinique du «miroir interne déformant de l'image de soi» : Le regard de l'enfant sur lui-même lui renvoie une image plus négative que ce que ses interlocuteurs ne perçoivent de lui. Dès le départ, l'enfant a une mauvaise perception de lui-même. Lorsqu'un interlocuteur lui fait une remarque, l'enfant bascule dans une image déformée et bien plus négative que celle que ne lui prête l'interlocuteur. Un dysfonctionnement de ces réseaux déséquilibre la balance et ne fait apparaître "qu'une seule couleur" dans le "miroir interne" par une restitution mnésique univoque : ou des attributs positifs, ou des attributs négatifs. Une prépondérance de l'image négative évoque un terrain dépressif, des personnalités évitante ou dépendante. Une prépondérance de l'image positive évoque des personnalités narcissiques, une image mégalomaniaque. Un dysfonctionnement neuronal peut induire une labilité et une instabilité de cette image (borderline), ou, une bascule rapide en "on-off" d'images positives et négatives de soi (rencontrée en pédopsychiatrie). Une psychoéducation permet la prise de conscience de ce miroir interne déformant. [less ▲]

Detailed reference viewed: 53 (1 ULg)
Peer Reviewed
See detailUltrasonography and histology of equine menisci; a comparative study of the medial
De Busscher,V; Gabriel, Annick ULg; Cassart, Dominique ULg et al

Poster (2007, January)

Detailed reference viewed: 9 (1 ULg)
Peer Reviewed
See detailLe "Mal-être en situation d'être seul" différencié de "l'Angoisse de séparation" : recherche sémiologique et élaboration conceptuelle
Scholl, Jean-Marc ULg; Philippe, Paule ULg

Poster (2007, January)

Une recherche sémiologique sur 170 enfants a individualisé : le "mal-être en situation d'être seul". Un malaise apparaît lorsque le sujet est seul. Voici son décours développementale. Certains bébés ... [more ▼]

Une recherche sémiologique sur 170 enfants a individualisé : le "mal-être en situation d'être seul". Un malaise apparaît lorsque le sujet est seul. Voici son décours développementale. Certains bébés réclament une présence physique pour s'endormir. Vers 2,5 ans, l'enfant connaît une séparation difficile lors des premiers jours d'école. Vers 5 ans, l'enfant n'aime pas jouer seul dans une pièce sans la présence d'une autre personne ou d'un animal, et s'il le fait, il cherche à savoir en permanence où se trouve l'adulte. À l'endormissement, l'enfant appelle souvent, sort de la chambre. La nuit, il voudrait rejoindre les parents. Vers 8 ans, il n'aura pas de difficultés à quitter la maison et à se séparer de ses parents pour aller jouer avec des copains du quartier, mais il sera beaucoup plus mal à l'aise si l'un de ses parents quitte momentanément la maison. Vers 9 ans, la symptomatologie se limite à celle retrouvée chez l'adulte : un sentiment vague de mal-être à l'endormissement. L'adulte a des difficultés à mettre des mots sur ce ressenti qui est sans représentation : du "vide" ou un "rien". Il cherche à retarder la mise au lit, à s'occuper au lit pour éviter ce ressenti, et à s'endormir rapidement de fatigue. L'adulte qui ressent ce "mal-être" en journée va le contrer par 4 moyens : 1. activités 2. apport sensoriel (radios ouvertes, TV), 3. remplir son "monde interne" par la lecture 4. un animal de compagnie. Le "mal-être en situation d'être seul" : A) Est à distinguer de l'angoisse de séparation B) Est corrélé à un retard d'apparition des repères temporels, non pas séquentiels ou notionnels, mais dans l'appréhension subjective du temps : "goûter anticipativement ce que dure un certain laps de temps" C) Il serait lié à un manque d'efficience d'un support neurophysiologique permettant l'évaluation de la longueur du temps : "la jauge interne d'évaluation anticipative du temps" D) Ceci expliquerait qu'il ne suffit pas à l'enfant d'avoir des représentations pour acquérir le sentiment subjectif d'une permanence mais qu'il faut y joindre ce "feeling" du temps E) Il est systématiquement présent dans la bipolarité sans en être pathognomonique. Il se retrouve à l'état isolé et dans d'autres entités nosologiques. A minima, il est une caractéristique de la personne. [less ▲]

Detailed reference viewed: 77 (3 ULg)
Peer Reviewed
See detailPectoral sound production in Synodontis (Mochokidae): a morphological study
Fabri, Gégory; Mauguit, Quentin; Vandewalle, Pierre ULg et al

Poster (2007)

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailFatty acids and stable isotopes in fish and harbour porpoises (Phocoena phocoena) from the North Sea: further insights in their trophic relationships
Drouguet, Olivier; Caut, Stéphane; Haelters, Jan et al

Poster (2007)

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises ... [more ▼]

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises displaying lesions linked to fishing nets has also increased this last decade. The increased abundance of porpoises together with the increase in numbers of bycaught animals raised the question of possible competition between porpoises and fishermen in the North Sea. Therefore, a detailed view on harbour porpoise trophic ecology in this region is crucial for their conservation. Stomachs of the washed ashore porpoises were often empty and provided limited information on their recent diet. Another way of looking into the diet of marine mammals is the analysis of blubber. Stable isotopes ratio (d13C and d15N, SI) and fatty acid composition (20 fatty acids, FA) were analyzed in muscle and blubber of 10 freshly stranded harbour porpoises and in 60 potential prey fish from 10 species collected in the North Sea. The SI ratio and FA composition of these fish enabled a clear classification in different trophic levels and different trophic niches. The FA composition was very similar between harbour porpoise blubber and demersal fish, underlining a preferential predation on that resource for the porpoises investigated. A bias might exist for the porpoises: most of the animals were juveniles, and washed ashore during spring. Porpoises might display other feeding habits in other life stages, other periods of the year. These preliminary results however indicate the enhanced accuracy of crossing these biomarker approaches. [less ▲]

Detailed reference viewed: 51 (6 ULg)
See detailRole of the transcription factor HNF-6 on doublecortin expression
Degueldre, Julie; Piens, Marie; Plumier, Jean-Christophe ULg

Poster (2007)

Detailed reference viewed: 4 (0 ULg)
See detailStrained Si-on-insulator for advanced CMOS devices
Mantl, S.; Buca, D.; Zhao, Q. et al

Poster (2007)

Detailed reference viewed: 8 (0 ULg)