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See detailMigration behaviour study of charged and uncharged compounds in micellar electrokinetic chromatography systems containing various proportions of SDS micelles and acetonitrile as organic modifier
Lamalle, Caroline ULg; Servais, Anne-Catherine ULg; Crommen, Jacques ULg et al

Poster (2012)

In 1984, Terabe and co-workers introduced a modified version of CZE, called micellar electrokinetic chromatography (MEKC), in which surfactant-formed micelles are included in the running buffer providing ... [more ▼]

In 1984, Terabe and co-workers introduced a modified version of CZE, called micellar electrokinetic chromatography (MEKC), in which surfactant-formed micelles are included in the running buffer providing a two phases chromatographic system for the separation of neutral compounds. Depending on their hydrophobicity, compounds can interact with the core of the micelles. Many MEKC methods have been described for the separation of neutral and basic compounds and for various applications. However, the migration behaviour of cationic, anionic and neutral analytes mixture is not well established regarding to the surfactant concentration and to the proportion of organic solvent in the background electrolyte (BGE). With such a mixture, it is important to remember that the separation of charged solutes in MEKC involves a combination of chromatographic and electrophoretic separation mechanisms. Moreover, it is worth noting that with charged analytes, two kinds of interactions with the micelles may occur; not only with the hydrophobic core but also with the head groups of the micelles through electrostatic interactions. In systems in which the surfactant has an opposite charge to that of the solute, ion pairing may occur. On the contrary, when a solute and the surfactant have similar charges, coulombic forces may cause the repulsion of these molecules. The goal of this study is to improve our understanding of the migration behaviour of charged and uncharged analytes in MEKC systems. With this aim in view, effective mobility (electrophoretic mobility under the influence of micelles) of neutrals, cations and anions were mesured at neutral, basic and acidic pH with BGE containing different SDS concentrations and ACN proportions. This study is also focused on the changes of migration order between CZE and MEKC systems using different BGE compositions. In the MEKC systems investigated in the study, SDS concentration and ACN proportion show a tremendous effect on the effective mobilities and migration order of the model compounds. While anions interact very weakly with SDS micelles, neutrals and cations interact with SDS through hydrophobic and ionic bonds. These interactions become stronger with the increase of SDS concentration and weaker with high ACN proportion. With 20 mM of SDS in the BGE, CZE behaviour is observed till 40% of ACN. But, when the SDS concentration is high and the ACN proportion is low, the migration order of analytes is reversed compared to CZE: EOF first, then the anions, followed by the neutrals and finally the cations. The migration order inside each group (cations, neutrals and anions) depends on the hydrophobicity of the analytes. Different organic solvents were also investigated to study the ion-pair formation. Those observations confirm the interest of using MEKC not only for the separation of neutral compounds but also variously charged analytes. [less ▲]

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See detailStudy of agronomic constraints to the dissemination of the cultivation of Jatropha curcas L. in Senegal
Terren, Marieke ULg; Saverys, S.; Jacquet de Haveskercke et al

Poster (2012)

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See detailMALDI In-Source Decay for High Throughput sequencing of peptide animal toxins
Quinton, Loïc ULg; Degueldre, Michel ULg; Gilles, Nicolas et al

Poster (2012)

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See detailEndocrine factors and AMPKα1 are involved in the spread of hatch and subsequent neonatal performance of broiler chicks
Wang, Yufeng; Li, Yue; Willems, Els et al

Poster (2012)

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See detailGeoarcheological aspects of new excavations at the Palaeolithic site of Kulbulak (Uzbekistan)
Flas, Damien ULg; Kolobova, K.; Pavlenok, K. et al

Poster (2012)

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See detailCreatinine and/or cystatin C-based GFR estimation in HIV-infected patients.
Gagneux-Brunon, A; DELANAYE, Pierre ULg; Fresard, A et al

Poster (2012)

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See detailHDAC5 depletion modulates heterochromatin plasticity and triggers programmed cell death of human cancer cells
Peixoto, P; Castronovo, V; Matheus, N et al

Poster (2012)

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See detailImpact of sequencing design on missing marker imputation and genomic selection in cattle
Druet, Tom ULg; MacLeod, Iona; Hayes, Ben

Poster (2012)

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See detailEvaluation of aptamers for targeted radiotherapy: Binding specificity and labelling with natural lutetium
Gijs, Marlies ULg

Poster (2012)

Many cancer patients suffer from serious side effects when treated with external beam radiotherapy or chemotherapy because of damage to healthy tissues by lack of selectivity. In this regard, specific ... [more ▼]

Many cancer patients suffer from serious side effects when treated with external beam radiotherapy or chemotherapy because of damage to healthy tissues by lack of selectivity. In this regard, specific targeting of tumours through radiopharmaceuticals is increasingly considered as a promising strategy in oncology. Radiopharmaceuticals consist of a radionuclide coupled to a vector that specifically targets cancer-related molecules. Aptamers are small (5-15 kDa) synthetic oligonucleotides (DNA or RNA) that possess several advantages compared to other vectors, such as an relatively easy and cheap chemical synthesis allowing the introduction of different chemical modifications, a selection possible against almost every target, a non-immunogenicity and a good tumour penetration. That is why aptamers are regarded as promising molecules for the development of radiopharmaceuticals. An aptamer targeting the Human Epidermal growth factor Receptor 3 (HER3), which plays an important role in cancer development and progression, was chosen for the development of aptamer-based radiopharmaceuticals. [less ▲]

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See detailWhetstones in Gaul: Building a typology
Thiebaux, Aurélie ULg; Goemaere, Eric; Deru, Xavier ULg et al

Poster (2012)

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See detailIMAGING FINDINGS IN HORSES WITH PHARYNGEAL SQUAMOUS CELL CARCINOMA
Etienne, Anne-Laure ULg; Evrard, Laurence ULg; Bolen, Géraldine ULg et al

Poster (2012)

Introduction Squamous cell carcinoma (SCC) has been occasionally reported in the equine pharyngeal region1-3. The aim of this poster is to describe imaging findings in 4 cases of pharyngeal SCC. Material ... [more ▼]

Introduction Squamous cell carcinoma (SCC) has been occasionally reported in the equine pharyngeal region1-3. The aim of this poster is to describe imaging findings in 4 cases of pharyngeal SCC. Material and methods Four old horses, mean age 19.5, 2 females and 2 geldings, were referred for dyspnea (3/4) and/or dysphagia (3/4). Because of dyspnea radiographs were realized prior to endoscopy. Ultrasound (US) was performed in all cases by ventral and lateral approach using a linear 7,5MHz transducer. A post-mortem computed tomography (CT) of the head was performed in one case (16 slices CT, Somatom 16, Siemens). Results Radiographic opacity of the pharyngeal region was increased in all cases. A soft tissue mass was also visible in the caudal maxillary sinus in 1 horse. The epiglottis was either not recognized or difficult to see with an abnormal shape. Pharyngoepiglottic distance and nasopharyngeal diameter were reduced in all cases. The soft palate was either thick or impossible to be outlined, with an irregular surface. In 1 case it was dorsally displaced. The dorsal pharyngeal wall looked unevenly thickened or impossible to be outlined ventrally due to border effacement. No bony damage was identified on radiographs. A hypoechoic heterogeneous mass was visualized at US in 2 cases and an enlargement of the mandibular lymph nodes was observed in 3 cases. Lymphnodes had also heterogeneous echogenicity and increased doppler signal in 1 case. Oral and pharyngeal endoscopic examination confirmed a pharyngeal mass in 2 cases but was unsuccessful or incomplete because of passage impairment in 2. CT revealed maxillary bone lysis in the horse with a mass in the maxillary sinus. Histopathological examination of local biopsies or necropsy revealed pharyngeal SCC invading epiglottis, pharyngeal wall and soft palate in the 4 horses and the maxillary sinus in one. Discussion/Conclusion Because endoscopy can be impaired by the size of the mass, radiology is helpful in estimating the extent and invasiveness of the process and US to confirm lymphadenopathy. However because of its relatively low sensitivity and the local increased opacity, radiographic examination may underestimate bone lysis. [less ▲]

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See detailHuntingtin mediates anxiety/depression-related behaviors and hippocampal neurogenesis.
M'Barek, K. Ben; Orvoen, S.; Pla, P. et al

Poster (2012)

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See detailAssessing the role of porosity in composite electrodes through model thin film electrode studies
Krins, Natacha ULg; Shukla, Alpesh K.; Milliron, Delia J. et al

Poster (2012)

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See detailPropofol induced unconsciousness: fMRI total neuronal activity and resting state networks.
Gantner, Ithabi; Guldenmund, Justus Pieter ULg; Gómez, Francisco et al

Poster (2012)

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See detailModeling of ligand induced changes in the magneto-structural properties of Pd13 cluster
Fresch, Barbara ULg; Remacle, Françoise ULg

Poster (2012)

Palladium nanostructures are widely used as catalyst of many organic reactions1 and present intriguing and controversial magnetic properties. Since the direct experimental determination of ground state ... [more ▼]

Palladium nanostructures are widely used as catalyst of many organic reactions1 and present intriguing and controversial magnetic properties. Since the direct experimental determination of ground state structure for small transition metal clusters is difficult, theory and first-principles calculations, such as density functional theory (DFT), have been used extensively to reveal the interplay between geometric and electronic structure. We present a theoretical study at the DFT/B3LYP level on three selected geometric arrangements for the Pd13 cluster with particular emphasis on the effects of thiolate and phosphine based ligands on their magneto-structural properties. The results point out that the interactions with ligands can change the relative stability of different structures of the metallic core, leading to the stabilization of the more compact icosahedral shape with respect to other bi-layer structures which are more stable in the free standing cluster. Beyond this structural evolution, the interactions with the ligand shell strongly modify also the electronic and magnetic properties of the metal core. In particular, both thiolate and phosphine ligands quench the high spin state that characterized the magnetic ground state of the bare Pd13 cluster, but while the interaction with phosphine quench the magnetic moment completely, the thiol-capped particles retain a permanent magnetic moment even when they are saturated by the ligand molecules. Our study suggests that different ligands can be effectively used to tune electronic and magnetic properties of Pd nanoparticles in view of the design and experimental realization of logic nano devices and intelligent sensors. [less ▲]

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See detailLa pêche aux nasses dans le delta du fleuve Ouémé, Bénin : implications pour une gestion rationnelle des ressources halieutiques
Montchowui, Elie; Agadjihouede, Hyppolite; Poncin, Pascal ULg et al

Poster (2012)

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See detailRegion- and context-specific rapid changes in brain aromatase activity following social interactions
de Bournonville, Catherine ULg; Dickens, Molly J; Ball, Gregory F et al

Poster (2012)

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See detailCellulase involvement in the bacterial cellulose biosynthesis
Delsaute, Maud ULg; Berlemont, Renaud ULg; Bauvois, Cédric et al

Poster (2012)

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See detailFire promotes downy brome (Bromus tectorum L.) seed dispersal
Monty, Arnaud ULg; Brown, Cynthia; Johnston, Danielle

Poster (2012)

Particularly well-known among the many impacts of the invasive annual grass downy brome (Bromus tectorum, Poaceae) is its ability to alter fire cycles and increase in abundance after fire. However, little ... [more ▼]

Particularly well-known among the many impacts of the invasive annual grass downy brome (Bromus tectorum, Poaceae) is its ability to alter fire cycles and increase in abundance after fire. However, little is known about how fire influences B. tectorum dispersal. We quantified fire effects on B. tectorum dispersal using three recently burned areas in the western region of the Colorado Rocky Mountains by marking diaspores (seeds) with fluorescent powder, and then recovering them at night using ultraviolet lights. Diaspores were of two types: with and without sterile florets attached. We also characterized vegetation cover and near-surface wind speed in burned and unburned areas. Diaspores travelled much farther in burned areas than in nearby unburned areas (means ± standard error at the end of the experiment: 209 ± 16 cm and 38 ± 1 cm, respectively; maximal distance at the end of the experiment: 2274 cm and 150 cm, respectively), indicating an increase in dispersal distance after fire. Diaspores with sterile florets attached dispersed longer distances than those without sterile florets (means ± standard error at the end of the experiment: 141 ± 14 cm and 88 ± 7 cm, respectively). Vegetation cover was lower and wind speeds were higher in the burned areas. Our results indicate that at least one of the mechanisms by which the spread of B. tectorum is promoted by fire is through increased seed dispersal distance. Preventing movement of seeds from nearby infestations into burned areas may help avoid the rapid population expansion often observed. [less ▲]

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See detailCombining in vivo and in silico experiments to unravel root water uptake dynamics
Lobet, Guillaume ULg; Bhowmick, Suman; Couvreur, Valentin et al

Poster (2012)

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See detailImplication of HDAC-5 in heterochromatin replication
Peixoto, P; Castronovo, V; Matheus, N et al

Poster (2012)

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See detailClinical Management and Resource Utilisation for Postmenopausal Hormone-Receptor-Positive HER2-Negative (HR+ HER2-) Advanced Breast Cancer (BC) in Europe
JERUSALEM, Guy ULg; MARINSEK, Nina; RICCI, Jean-François et al

Poster (2012)

Objective: To understand treatment patterns and quantify resource utilisation of HR+ HER2– Advanced BC, with the overall aim of comparing costs and disease burden as patients progress from hormonal ... [more ▼]

Objective: To understand treatment patterns and quantify resource utilisation of HR+ HER2– Advanced BC, with the overall aim of comparing costs and disease burden as patients progress from hormonal therapy (HT) to chemotherapy (CT). Methods: We conducted a chart audit in France, Germany, The Netherlands, Belgium, and Sweden of 399 living and deceased postmenopausal female patients (target, 75 per country) diagnosed with HR+ HER2– advanced BC in the past 4 years. Patients were required to have progressed on ≥ 1 line of prior HT either in the adjuvant or advanced setting and to have completed ≥ 1 line of CT (minimum 2 full cycles) in the advanced BC setting. The chart audit was completed online using a standardised form developed with the assistance of European academic physicians, pharmacy directors, and hospital administrators. Participation was sought from 25 oncologists per country, except in Germany (15 oncologists and 10 gynecologists to reflect local clinical practice). Study was compliant with European and country market research regulations. Results: Our report details the patient care pathway, CT side effects, and resource utilisation in the inpatient and outpatient settings throughout the continuum of advanced BC care. In the study sample 55% of HR+ HER2– advanced BC patients are first treated with HT and switch to CT after an average of 1.5 lines of HT. This switch is primarily influenced by the extent (56%) and progression rate (36%) of metastases. The switch from HT to CT is associated with increased resource utilisation of treating advanced BC. Conclusions: Our results highlight the increased resource utilisation for postmenopausal HR+ HER2– advanced BC patients treated with CT versus HT. [less ▲]

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See detailDating methods applied to building archaeology
Blain, Sophie ULg; Guibert, Pierre; Hoffsummer, Patrick ULg et al

Poster (2012)

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See detailExtensions of the Background/biResonant decomposition
Denoël, Vincent ULg

Poster (2012)

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See detailCortical interneurons tangential migration : p27(Kip1) as a novel master regulator.
Godin, Juliette ULg; Thomas, Noémie; Laguesse, Sophie ULg et al

Poster (2012)

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See detailThe P3 variability across healthy subjects using an auditory paradigm
Chatelle, Camille ULg; Lulé, Dorothée; Kübler, Andrea et al

Poster (2012)

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See detailGlycine receptor activation controls interneuron migration by affecting nuclear translocation and myosin phosphorylation
Avila Macaya, Ariel Salvatore ULg; Nguyen, Laurent ULg

Poster (2012)

Previous studies have described the presence of glycine receptor mRNA during early stages of embryonic cortex development. Here, we have tested the functionality of those receptors in migratory ... [more ▼]

Previous studies have described the presence of glycine receptor mRNA during early stages of embryonic cortex development. Here, we have tested the functionality of those receptors in migratory interneurons and demonstrated their involvement in the control of cell migration. We suggest a mechanism whereby activation of glycine receptors during tangential migration activates voltage gated calcium channels and favors influx of calcium that ultimately affect myosin II activity, a mechanism that fine tune nuclear translocation and thus migration speed. [less ▲]

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See detailLe débit de filtration glomérulaire est-il un déterminant de la concentration plasmatique du NGAL aux soins intensifs ?
DELANAYE, Pierre ULg; Claisse, Guillaume; Mehdi, Manoli et al

Poster (2012)

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See detailMicellar electrokinetic chromatography (MEKC) systems for the separation of mixtures of charged and uncharged compounds
Lamalle, Caroline ULg; Servais, Anne-Catherine ULg; Fradi, Inès et al

Poster (2012)

The migration behaviour of charged and uncharged analytes in MEKC was investigated under different conditions. Effective mobilities – electrophoretic mobilities under the influence of the negatively ... [more ▼]

The migration behaviour of charged and uncharged analytes in MEKC was investigated under different conditions. Effective mobilities – electrophoretic mobilities under the influence of the negatively charged micelles – of cations, anions and neutrals were measured at neutral, basic and acidic pH values (7.5, 11 and 2.2) using background electrolytes containing different sodium dodecyl sulfate (SDS) concentrations (0-90 mM) and acetonitrile proportions (0-75 %, v/v). The SDS concentration and acetonitrile proportion were found to have a tremendous effect on the effective mobilities and the migration order of the tested compounds. Although the SDS micelles interact more strongly with neutrals and cations, the migration of anionic compounds is also affected by the SDS concentration, indicating that hydrophobic interactions can occur between the micelles and these compounds. Since cationic, anionic and neutral solutes exhibit rather different migration behaviours, it is possible to considerably enhance the separation selectivity by properly adjusting the SDS concentration and the acetonitrile proportion in the background electrolyte. These observations confirm the interest of using MEKC not only for the separation of neutral substances but also for the analysis of mixtures of charged compounds. [less ▲]

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See detailAdvanced genetic models for Piétrain boars involved in crossbreeding in the Walloon Region using test station and on-farm phenotypic and genomic data
Dufrasne, Marie ULg; Jaspart, Véronique; Wavreille, José et al

Poster (2012)

Since 2007 the Walloon Region is acquiring a new advanced genetic evaluation program for Piétrain boars. Comprehensive models for routine genetic evaluation of boars involved in crossbreeding programs ... [more ▼]

Since 2007 the Walloon Region is acquiring a new advanced genetic evaluation program for Piétrain boars. Comprehensive models for routine genetic evaluation of boars involved in crossbreeding programs, using simultaneously data recorded on crossbred progeny at central test station and data recorded on-farm on purebred or crossbred pigs, are under development. Traits evaluated include growth, carcass quality traits and feed intake. Estimated breeding values allow breeders to select the best boars according to their selection objectives. But to have comprehensive models for crossbreeding performances, more advanced modelling of non-additive and crossbreeding effects will be added to current models. Additionally potential use of genomic information will be explored. [less ▲]

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See detailPrenatal undernutrition of the chicken embryo leads to changes in plasma T3 and corticosterone levels
Willems, Els; Wang, Yufeng; Willemsen, Hilke et al

Poster (2012)

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See detailBone marrow-derived mesenchymal stromal cells failed to prevent experimental xenogeneic graft-versus-host disease
Bruck, France; de Leval, Laurence; Belle, Ludovic ULg et al

Poster (2012)

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See detailTwo novel equations to estimate kidney function in persons aged 70 years or older
Schaeffner, ES; Ebert, N; DELANAYE, Pierre ULg et al

Poster (2012)

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See detailHygrothermal modelling of Lime-Hemp concrete used as building material and indoor climate buffering characterization
Dubois, Samuel ULg; Evrard, Arnaud; Lebeau, Frédéric ULg

Poster (2012)

Hygroscopic building materials can affect the indoor air by exchanging moisture with it and buffering the ambient humidity variations. The Nordtest protocol and the concomitant Moisture Buffer Value (MBV ... [more ▼]

Hygroscopic building materials can affect the indoor air by exchanging moisture with it and buffering the ambient humidity variations. The Nordtest protocol and the concomitant Moisture Buffer Value (MBV) parameter definition has been one of the first attempt to characterize this moisture regulation performance. This paper present an attempt to confront this well-established experimental protocol with a mathematical model implemented in the Comsol multiphysics solver. The power of the simulation will be showed with a good correlation between the modelling and the experimental phase for the MBV determination of a lime-hemp concrete bloc. [less ▲]

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See detailEffects of Physical Activity on the Aging of Motor and Perceptual Inhibition
Albinet, Cédric; Boucard, Grégory; Collette, Fabienne ULg et al

Poster (2012)

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See detailGenetic variation in heat stress tolerance of Holsteins producing under a continental temperate environment
Hammami, Hedi ULg; Arnould, Valérie ULg; Bormann, Jeanne et al

Poster (2012)

Under projected climate changes, considerations for heat stress tolerance but also for genotype by environment interactions should enter breeding decisions, both for importing, but also exporting ... [more ▼]

Under projected climate changes, considerations for heat stress tolerance but also for genotype by environment interactions should enter breeding decisions, both for importing, but also exporting countries. Most studies concerning the breeding strategies on heat stress tolerance used the temperature humidity index (THI) to assess the thermal stress in a given environment and assumed a specific threshold to each studied population with the same response to each individual above this threshold. Recently new comprehensive thermal indices (TI) integrating radiation solar, wind speed, in addition to temperature and relative humidity were developed. Therefore, the aim of this study is advanced modelling of milk yield trait to evaluate the genetic variation in heat stress tolerance of Holsteins in a temperate environment using random regressions models and six new comprehensive TI. A total of 107,350 test day milk records were available for 12,099 primiparous Holsteins calving from 2000 to 2010. Test-day milk records were merged with meteorological data from 14 public weather stations across Luxembourg. Daily values of 6 new comprehensive TI (3 THI and 3 apparent temperature indices) were calculated by averaging hourly TI over 24 hours. The average distance between herds and their meteorological reference station was of 16 km. Sensitivity of cows to the climate environmental conditions was modelled by applying a reaction norm for each animal, representing its EBV for milk yield on values of the TI on the day in milk (DIM). Six separate random regression models were applied using time-dependent (DIM) and specific TI-dependent covariates. Random effects were additive genetic, permanent environmental, and herd year modelled with Legendre polynomials of order 2 for both DIM and TI. Genetic variances of the different TI evaluated in this study represent 27 to 30% of their correspondent variances for DIM effect. Permanent environmental and genetic variances of TI effect were in the same range. Additive genetic variances and heritabilities for daily milk yield slightly decreased with increasing degrees of the different THI and apparent temperature indices. The average daily milk heritability overall the lactation at the mean of each of the six TI was of 0.28. Genetic correlations between adjacent points across the lactation were > 0.90. Correlations between extreme distant DIM (e.g. early and late lactation stage) were reduced at 0.38 to 0.52. The overall genetic correlations between the different values of each TI remain high (> 0.85). EBV’s summing regular additive effect (DIM effect) and tolerance to heat stress (TI effect) were computed for several combinations of DIM and TI values. Ranking for sires and cows did not change among the 6 heat stress indicators. The correlations between EBVs estimated with the six models were greater than 0.90. In conclusion, the different models depicted genetic variation of milk yield among combinations of DIM and TI levels without defining thresholds in advance. Genetic variation was slightly lower when TI values exceed the thermoneutral zone. Breeding for reduced thermal sensitivity using any of the six indicators of heat stress evaluated in this study is possible. The indicator TI2 representing the adjusted THI for radiation solar and wind speed is favoured because it was the indicator that did identify the highest yearly milk losses under this continental temperate environment. [less ▲]

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See detailThe histone variant macroH2A regulates the commitment of embryonic and adult stem cells
Creppe, Catherine ULg; Cantariño, N; Janich, P et al

Poster (2012)

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See detailTechnical pitfalls in cardiac CT angiography : what the radiologist should know
GHEKIERE, O; DJEKIC, JULIEN; MANCINI, ISABELLE et al

Poster (2012)

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See detailOxygen transfer in inverse fluidized bed during biosurfactant production by Bacillus subtilis
Fahim, S.; Dimitrov, K.; Vauchel, P. et al

Poster (2012)

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See detailIn silico genome mining, a new approach to detect novel lipopeptides potentially involved in biological control
Leclere, V.; Krier, F.; Deravel, J. et al

Poster (2012)

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See detailCKD-EPI GFR estimating equations in kidney transplantation: which added value for the new cystatin C based equations?
Masson, I; Maillard, N; Kamar, K et al

Poster (2012)

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See detailSpotlight on Babesiosis in Belgium
Lempereur, Laetitia ULg; Shiels; Saegerman, Claude ULg et al

Poster (2012)

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See detailEffet de l'érythromycine sur le traitement par boues activées
Louvet, Jean-Noël ULg; Giammarino, Cinzia; Dumas, Dominique et al

Poster (2012)

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See detailDispersal and regeneration capacity of large-seeded tree species in a forest-savanna mosaic in Western DR Congo
Trolliet, Franck ULg; Huynen, Marie-Claude ULg; Hambuckers, Alain ULg

Poster (2012)

It is widely recognized that the Congo Basin is affected by numerous anthropogenic pressures. A number of studies proved that hunting and forest fragmentation diminish the diversity and abundance of large ... [more ▼]

It is widely recognized that the Congo Basin is affected by numerous anthropogenic pressures. A number of studies proved that hunting and forest fragmentation diminish the diversity and abundance of large vertebrates, more specifically, of large frugivores. The depletion of those animals can directly affect large-seeded tree species as large seeds closely depend on the community of large frugivorous vertebrates for their dispersal. Then, the disruption of animal mediated seed dispersal is thought to deeply impact the plant regeneration capacity. The forest-savanna mosaic situated in Western DR Congo is an ecotone characterized by naturally occurring forest fragments which are also subject to numerous anthropogenic pressures. Those are very likely to disrupt seed dispersal mechanisms and to alter forest regeneration processes. To date, few studies have considered the effect of such an ecosystem on plant-animal interaction dynamics such as seed dispersal, and none have been done in this region. This study will examine if the early stages of regeneration of the large-seeded tree species Anonidium mannii, namely the dispersal capacity and seedling establishment is affected by forest fragment size. We predict that the small fragment size will negatively affect the regeneration capacity of this species. To test this assumption, we will work along a gradient of forest fragment sizes to define the composition of the seed disperser communities. For each disperser assemblage, we will evaluate the quantitative capacity of seed dispersal by combining direct focal observations and camera trapping. A literature review on each disperser species seed retention time and habitat use will allow the computation of the seed dispersal kernels. We will also evaluate the effect of seed ingestion by a presumably important seed disperser, the bonobo, Pan paniscus, on its germination capacity. Seeds will be collected from dung to evaluate the effect of seed ingestion on the rate and velocity of germination. Finally, we will study the predation pressure exerted on dispersed and non-dispersed seeds and seedlings by setting up two sets of seeds below the canopy of parent trees and away from any conspecific trees. One set will be dispersed unprotected to seed predators; another one will be enclosed in a cage and permit seeds to germinate, allowing us to evaluate the herbivores pressure on seedlings. [less ▲]

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See detailThe sheepshead minnow Cyprinodon variegatus as a marine vertebrate model for investigating endocrine disrupting effects of triclosan
Schnitzler, Joseph ULg; Gauthray, Ghislain ULg; Silvestre, Frédéric et al

Poster (2012)

Zebrafish (Danio rerio) has been a prominent model in toxicology. This freshwater species present many advantages both in identifying endpoints of toxicity and in elucidating mechanisms of toxicity ... [more ▼]

Zebrafish (Danio rerio) has been a prominent model in toxicology. This freshwater species present many advantages both in identifying endpoints of toxicity and in elucidating mechanisms of toxicity. However, marine toxicologists lack such omnipotent model displaying similar advantages to zebrafish: small size, rapid breeding, and easy maintenance. We propose here the use of the sheepshead minnow Cyprinodon variegatus to define thyroid-disrupting effects of triclosan during pre-hatching development. Three females and two males were placed in each of the three breeding chambers designed for this experiment. More than 300 eggs were collected over three days and maintained in seawater with triclosan concentrations at 0, 100 ng.L-1, and 100 µg.L-1. Exposure of larvae to triclosan lasted until hatching. After hatching (six days post-spawning), 5 larvae per condition were placed in Bouin’s fixative for brain and thyroid histology. Length and body mass were measured. The body was frozen at -80oC to determine muscle thyroid hormone levels (T3 and T4), using radio-immunoassay techniques. Hatching success, in vivo observations, gross observation, thyroid hormone levels, histology will be determined and discussed in the framework of whether or not triclosan may alter thyroid metabolism during pre-hatching period of the sheepshead minnow. [less ▲]

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See detailRegeneration pos-fire of herbaceous communities of campos rupestres (tropical altitude grasslands)
Hernandez, Pauline; Le Stradic, Soizig ULg; Buisson, Elise et al

Poster (2012)

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See detailOncogenic human papillomavirus could directly interact with natural killer cells
Renoux, V; Bastin, R; Langers, I et al

Poster (2012)

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See detailNeuroimaging of emotional stroop task performance and mood changes in bipolar disorders
Rey, Gwladys; Desseilles, Martin ULg; Favre, Sophie et al

Poster (2012)

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See detailBlue... is a bad sign
LORCE, Aurélie ULg; BODSON, Lucien ULg; Ghuysen, Alexandre ULg et al

Poster (2012)

Detailed reference viewed: 96 (9 ULg)
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See detailPerformance, limitations and utility of cystatin C as an endogenous GFR marker in renal transplantation
Masson, I; Maillard, N; Tack, I et al

Poster (2012)

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See detailStudy of HCV and HIV infections in Mali: Comparative Epidemiology and Risk Factors
BOUHARE, NOUHOUM; VAIRA, Dolorès ULg; GOTHOT, André ULg et al

Poster (2012)

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See detailConstruction and pre-validation of a cognitive ergonomics questionnaire for work holding in multiple sclerosis patients : The QUIPSEP
DELRUE, Gaël ULg; MONISSE, Aurélie; Blavier, Adelaïde ULg et al

Poster (2012)

We describe how we build and pre-validate a cognitive ergonomics questionnaire for multiple sclerosis patients actually employed, the QUIPSEP. This questionnaire must help us better manage our patients ... [more ▼]

We describe how we build and pre-validate a cognitive ergonomics questionnaire for multiple sclerosis patients actually employed, the QUIPSEP. This questionnaire must help us better manage our patients and thei potentiel difficulties in work situation from a cognitive ergonomic point of view. [less ▲]

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See detailProduction laitière et nombre de traites de vaches laitières en prairie avec un robot: effets des facteurs environnementaux.
Dufrasne, Isabelle ULg; Robaye, Vincent ULg; Knapp, Emilie ULg et al

Poster (2012)

En Belgique, comme dans d'autres pays européens, le recours à la traite robotisée est en augmentation. La majorité des troupeaux traits avec un robot ne pratique pas le pâturage car les exploitants ... [more ▼]

En Belgique, comme dans d'autres pays européens, le recours à la traite robotisée est en augmentation. La majorité des troupeaux traits avec un robot ne pratique pas le pâturage car les exploitants considèrent que cette méthode n'est pas conciliable avec le pâturage. La fragmentation des parcelles est aussi un frein au pâturage. La mise en place d'un robot dans une structure mobile permet d'utiliser le même robot à l'étable en hiver et dans les prairies distantes de l'exploitation en été. L'objectif de cette étude est de tester l'effet de facteurs environnementaux, l'animal, la complémentation, la distance entre le robot et la parcelle, les numéros du jour dans la parcelle et de cycle de rotation sur la production laitière (PL) et le nombre de traites (NT). [less ▲]

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See detailDevelopment of a regional-scale library of near infrared reflectance soil spectra for alternative assessment of soil characteristics in Southern Belgium
Genot, Valérie ULg; Dardenne, Pierre; Bock, Laurent ULg et al

Poster (2012)

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as ... [more ▼]

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as methodological or technical prospective are realized under scientifically supervision of our research laboratory. In this context, a study was conducted to evaluate the ability of the NIRS to predict some soil properties: CEC, TOC, TN and clay content. The initial models were elaborated upon local PLS regression on set of 1 300 soil samples. The local PLS calibration used allows an accurate prediction of the soil properties and precision of NIRS technique is comparable to reference analytical method [less ▲]

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See detailRééducation attentionnelle chez l’enfant avec Trouble Déficitaire de l’Attention (TDA) : Analyse de deux cas.
Turine, Hélène ULg; Lejeune, Caroline ULg; Catale, Corinne ULg

Poster (2012)

L’objectif de cette étude est d’évaluer l’efficacité d’une prise en charge cognitive basée sur des exercices spécifiques d’entraînement de l’attention (informatisés et papier-crayon) sur les performances ... [more ▼]

L’objectif de cette étude est d’évaluer l’efficacité d’une prise en charge cognitive basée sur des exercices spécifiques d’entraînement de l’attention (informatisés et papier-crayon) sur les performances cognitives et le comportement de deux enfants TDA. L’impact d’une prise en charge attentionnelle chez des enfants avec TDA reste, à ce jour, mal connu même si quelques rares travaux suggèrent un bénéfice de ce type d’intervention sur des mesures attentionnelles et comportementales chez ces enfants (e.g., Noël et al., 2007). Deux enfants TDA avec troubles attentionnels objectivés par un examen neuropsychologique ont bénéficié de 40 séances de rééducation, lesquelles proposaient des exercices spécifiques aux difficultés de ces deux enfants (inhibition, attention soutenue et sélective et flexibilité). Chaque enfant était apparié à quatre enfants de contrôle. Les analyses de comparaison pré et post-rééducation montrent une amélioration des performances pour le cas 1 au niveau de différents composants attentionnels (inhibition, attention soutenue, attention sélective et flexibilité) ainsi qu’au niveau comportemental (p<.05) qui se maintient au-delà de 6 mois après la rééducation. En revanche, les analyses réalisées pour le cas 2 ne montrent aucune amélioration mais plaident en faveur d’un changement de stratégie cognitive (amélioration de la performance qualitative au détriment de la vitesse de réalisation). Cette étude confirme l’intérêt d’une prise en charge attentionnelle chez des enfants avec TDA et souligne l’importance de différents paramètres (variabilité interindividuelle, âge, motivation, profil attentionnel,…) dans le succès d’une telle rééducation. [less ▲]

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See detailHCV screening in African (Malian) women : relevancy of the HCV NS3 epitope
BOUHARE, NOUHOUM; VAIRA, Dolorès ULg; GOTHOT, André ULg et al

Poster (2012)

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See detailA RAPID VALIDATED UHPLC-PDA METHOD FOR ANTHOCYANINS QUANTIFICATION FROM EUTERPE OLERACEA FRUITS
Dias, A.L.S.; Rozet, Eric ULg; Chataigne, G et al

Poster (2012)

Commercialization of Euterpe oleracea fruit has increased because of its abundance in anthocyanins [1]. Characterizations of these compounds are important for the food industry. The aim is to validate an ... [more ▼]

Commercialization of Euterpe oleracea fruit has increased because of its abundance in anthocyanins [1]. Characterizations of these compounds are important for the food industry. The aim is to validate an UHPLC-PDA method for major anthocyanins quantification in this fruit after fast extraction procedures and samples preparation. Fruits were harvested in Abaetetuba (Brazil) and extracted sequencially by EtOAc, MeOH and MeOH 50% all at 0.1% HCl. A HSS C18 column (1.8µm) was used with a gradient elution of ACN and 5% HCOOH. Total error and accuracy profiles were used as validation criteria. A first EtOAc extraction removes the lipophilic compounds and allows an easier extraction by MeOH and quantification of anthocyanins in this extract. It was found to be faster (17 min) that HPLC-UV methods [2]. Calibration in the matrix was found to be more accurate than calibration without matrix. Trueness (<6.76% relative bias), repeatability (<4.6% RSD), intermediate precision (<5.3% RSD), selectivity (by UHPLC-ESI+-HRMS), response function and linearity for cyanidin-3-glucoside and cyanidin-3-rutinoside were evaluated. The concentration range validated was 1 to 48 µg/mL for both compounds. [less ▲]

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See detailAction parsing in compulsive checking
Belayachi, Sanaa ULg; Van der Linden, Martial ULg

Poster (2012)

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See detailCognitive Flexibility in Mood Disorders
Piguet, C.; Strepenich, V.; Desseilles, Martin ULg et al

Poster (2012)

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See detailAssessing the role of porosity in composite electrodes through model thin film electrode studies
Krins, Natacha ULg; Shukla, Alpesh K.; Milliron, Delia J. et al

Poster (2012)

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