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See detailNicotine restores endothelial dysfunction caused by excess sFLT1 and sEng in in vitro model pf preeclamptic vascular endothelium
Mimura, Kazuya; Tomimatsu, Takuji; Sharentuya, Namuxila et al

Poster (2009)

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See detailMCF-7/BOS cells membrane proteome: comparison of two isolation methods using mass spectrometry
Bertrand, Virginie ULg; Massart, Anne-Cécile ULg; De Pauw, Marie-Claire ULg et al

Poster (2009)

Membrane proteins (MP) play an important role in biological processes. Isolation and quantification of these MP using classical techniques is often limited due to their poor solubility and relatively low ... [more ▼]

Membrane proteins (MP) play an important role in biological processes. Isolation and quantification of these MP using classical techniques is often limited due to their poor solubility and relatively low abundance. These membrane proteins enclosed markers which could be potential therapeutic targets. These potential therapeutic targets have to be accessible to antibodies and need to be presented in the plasmic membrane. Assays were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain an enriched membrane fraction to facilitate the analysis of the sample and to simplify the complex proteins mixture. To isolate transmembrane proteins, we compared two methods. The first one used different extraction cycles characterized by different buffers to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. The obtained enriched membrane proteome was digested with trypsin and/or Lysyl Endopeptidase. Obtained peptides were separated by 2D-HPLC chromatography and on-line analysed ion trap mass spectrometer, the Esquire HCT. [less ▲]

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See detailEcological modeling and paedomorphosis: a study case in Montenegrin newts
Denoël, Mathieu ULg; Ficetola, Gentile; Cirovic, Rusa et al

Poster (2009)

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See detailReal-time spatial analysis of root water uptake in rhizotrons
Lobet, Guillaume ULg; Javaux, M.; Pages, L. et al

Poster (2009)

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See detailEmission line stars in the Milky Way with the GAIA space mission
Martayan, C.; Frémat, Y.; Blomme, R. et al

Poster (2009)

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See detailResponse of floodplain sedimentation to catchment disturbances in different environments
Notebaert, Bastiaan; Houbrechts, Geoffrey ULg; Verstraeten, Gert et al

Poster (2009)

Holocene floodplain sediments are an important environmental archive, that can be accesed for reconstructing the past landscape dynamics either qualitatively (e.g. palynology) and quantitatively (e.g ... [more ▼]

Holocene floodplain sediments are an important environmental archive, that can be accesed for reconstructing the past landscape dynamics either qualitatively (e.g. palynology) and quantitatively (e.g. sediment budgeting). In this study Holocene alluvial sediment deposition in two contrasting Belgian catchments was quantified and dated: the Lienne (148 km²) in the Ardennes massif and the Dijle (750 km2) in the loess region. These catchments experienced a comparable Holocene climatic variation, but differ in topography and geology with highest relief energy in the Lienne catchment. Land use history also differs with high land use intensities in the Dijle catchment since Roman times, but at least since the Middle Ages there were also large deforestations in the Lienne catchment. Detailed cumulative Holocene sediment deposition was assessed for each catchment using more then 1000 hand augerings. Detailed radiocarbon dating of fluvial deposits was performed in the Dijle catchment, while iron slag was used as a tracer for sediments deposited after 1350 AD in the Lienne catchment. Results show that sediment deposition is much larger in the Dijle catchment (~4.5 Mg ha-1 catchment area) then in the Lienne catchment (~0.2 Mg ha-1 catchment area). Dating results from the Dijle catchment show an increase of sediment deposition in the late Holocene, first starting in the colluvial valleys and later on prograding towards the main valleys. Variations in sedimentation rates can clearly be related to anthropogenous land use pressure, and the majority of the sediments found in colluvial and alluvial valleys were deposited in the last 4000 years, and in many cases even in the last 1000 years. Variations in sediment deposition within the catchment can partially be explained by differences in river valley physical settings (mainly valley slope), while in other cases hill slope sediment delivery (upstream erosion, connectivity between hill slopes and the river system) is the explaining factor. In the Lienne catchment, between 80 and 100% of the sediments present in the main valley were deposited before 1350AD, thus before the main period of forest clearance. On the other hand, about 50% of the Holocene sediment present in a main tributary was deposited after 1537 (local start of iron industry). This difference with the main valley can be explained by a difference in physical settings of the river (valley slope, stream power). These results show that the physical settings of river valleys determine how sensible the river systems are to catchment disturbances. Floodplain deposition is determined by an interplay of hill slope sediment delivery (hill slope erosion and connectivity) and physical settings of the valley itself (valley slope, discharge). As a result, quantification of floodplain deposition alone, cannot be used as a measure for historic soil erosion within a catchment or to determine intensity of human impact on the catchment. [less ▲]

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See detailOutreach goals of the Europa Jupiter System Mission
Blanc, M.; Coustenis; Nazé, Yaël ULg et al

Poster (2009)

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See detailImpact de l’anthropisation sur la composition paysagère de l’Ile Mbiye en R.D. Congo.
Bamba, I; Shimba, N; Iyongo, L et al

Poster (2009)

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See detailEffect of phospholipids on surface properties of rapeseed oleosins at two pH
Vaca Medina, Guadalupe; Mouloungui, Zéphirin; Roiz, Julie ULg et al

Poster (2009)

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See detailInnate lymphocytes in human papillomavirus (HPV)-associated cervical cancers
Renoux, V; Bisig, B; Thiry, Marc ULg et al

Poster (2009)

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See detailResistance of pearlfishes to saponins
Maïté, Todesco; Parmentier, Eric ULg; Eeckhaut, Igor

Poster (2009)

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See detailMolecular phylogeny of symbiotic pearlfishes
Lanterbcq, D; Parmentier, Eric ULg; Todesco, Maïté et al

Poster (2009)

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See detail4th International Symposium on Chemosynthesis-based Ecosystems
Ponsard, J; Cambon-Bonavita, M-A; Lepoint, G et al

Poster (2009)

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See detailFluctuation of sediment production during the last millennia in the Ardenne Massif (Belgium)
Houbrechts, Geoffrey ULg; Notebaert, Bastiaan; Petit, François ULg et al

Poster (2009)

In the Ardenne massif, floodplains have recorded fluctuations in the production of sediment and several periods of increased sediment deposition have been identified. Before the first deforestations ... [more ▼]

In the Ardenne massif, floodplains have recorded fluctuations in the production of sediment and several periods of increased sediment deposition have been identified. Before the first deforestations rivers developed multiple channels in alluvial forests. Floodplains were not well-developed and probably very humid, which explains the presence of peat layers within the alluvial sequences. In the Amblève catchment, the first increased sedimentary deposition of the Holocene occurred during the Bronze Age (3200 BP), in relation to deforestation and the first crop cultures in the area. An organic deposition has occurred around 2700 BP and could correspond with a period of climatic degradation. Several peat layers have also been dated to around 1000 BP and probably indicate very low anthropogenic pressure. From the 11th Century onwards, there was an increase in sedimentation and a greater concentration of charcoal is present in the alluvial deposits. In many catchments there is an important increase in the sedimentation at the end of the 14th century, which can be related to the development of many iron factories. Analyses of slag concentration produced in these former factories allow us to reconstruct the evolution of the floodplain topography since the inception of the iron industries, in relation to the periods of activity. Total sedimentation in the smaller valleys since the initiation of iron industries amounts 0.5 to 1.0 m, which is in most cases about 50% of the total sediment present in the floodplains and corresponds to a mean sedimentation rate ranging between 10 and 20 cm/century. Such values are explained by various former agricultural practices and forest clearings associated with the huge demand for charcoal by the iron industry. About 20 ha of forest were cleared for the yearly consumption of a refining forge or a blast furnace and more than three hundred iron factories existed in the Ardenne Massif between the 14th and the 19th century. [less ▲]

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See detailCan Nicotine become the remedy for the Pregnancy Induced Hypertension Syndrome?-A basic Research-
Sarun, Tuya; Tomimatsu, Takuji; Mimura, Kazuya et al

Poster (2009)

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See detailPhysicochemical characterization of fat blends related to margarine formulation
Danthine, Sabine ULg; Fernando Munoz, J; Cavillot, V et al

Poster (2009)

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See detailNicotine suppresses interleukin-6 production from vascular endothelial cells
Tomimatsu, Takuji; Sharentuya, Namuxila; Mimura, Kazuya et al

Poster (2009)

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See detailBAM and PM-IRRAS analyses of the alamethicin interfacial organization in phospholipid monolayers
Kouzayha, Achraf; Nasir, Mehmet Nail ULg; Besson, Françoise

Poster (2009)

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See detailUse of somatic hybridization to look for aromatic liness of Abies : preliminary results
Misson, Jean-Pierre; Thomas, Cynthia; Roblain, N. et al

Poster (2009)

Objectif : Associer les caractéristiques de croissance d’Abies nordmanniana aux propriétés aromatiques d’Abies balsamea Axes de recherche: - Hybridation somatique à partir de protoplastes d’Abies balsamea ... [more ▼]

Objectif : Associer les caractéristiques de croissance d’Abies nordmanniana aux propriétés aromatiques d’Abies balsamea Axes de recherche: - Hybridation somatique à partir de protoplastes d’Abies balsamea et Abies nordmanniana. - Détermination des profils aromatiques comme moyen de criblage. [less ▲]

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See detailHydroxyl detection on Venus and Earth and implications for ozone
Migliorini; Piccioni, G.; Cardesin-Molino, A. et al

Poster (2009)

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes ... [more ▼]

We present a comparison between hydroxyl night side infrared emissions on Earth and Venus. The vertical brightness distributions of the OH emissions are shown for the two planets and excitation processes are discussed. [less ▲]

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See detailAntioxidant and cytotoxicity activity of essential oil grown Lavandula dentata
Imelouane, B.; El Bachiri, A.; Wathelet, Jean-Paul ULg et al

Poster (2009)

The present study describes the phytochemical profile Antioxidant and cytotoxicity activity of Lavandula dentata essential oil, collected in eastern Morocco (Taforalt). The sample of essential oil was ... [more ▼]

The present study describes the phytochemical profile Antioxidant and cytotoxicity activity of Lavandula dentata essential oil, collected in eastern Morocco (Taforalt). The sample of essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC–MS. The in vitro cytotoxicity of the oil on the cancer cell lines PC-3 (prostate), V79 (fibroblaste) and normal P388D1 (murine macrophage) was examined. We have shown that the oil had negligible cytotoxic effects against all cell lines tested. The oil was also found to possess antioxidant activity as demonstrated by the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical method. Lavandula dentata essential oil has promising potentials for incorporation into various food, cosmetic and pharmaceutical products for which a natural aroma, colour, antioxidant and antimicrobial additive is desired. [less ▲]

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See detailSpecies delimitation and phylogeography of an African tropical tree species complex
Duminil, J.; Heuertz, M.; Bourland, Nils ULg et al

Poster (2009)

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See detailStudy of Essential Oils of Some Plants from Algeria (Salvia officinalis, Thymus vulgaris and Ruta chalepensis)
Brada, M.; Achour, D.; Wathelet, Jean-Paul ULg et al

Poster (2009)

The essential oil of three plants (Salvia officinalis, Thymus vulgaris and Ruta chalepensis) collected from Chlef, in the north west of Algeria, was isolated by steam distillation and investigated by GC ... [more ▼]

The essential oil of three plants (Salvia officinalis, Thymus vulgaris and Ruta chalepensis) collected from Chlef, in the north west of Algeria, was isolated by steam distillation and investigated by GC and GC/MS. The yield of essential oil (g/100g) was 1.6% for Salvia officinalis, 2.6% for Thymus vulgaris and 1.1% for Ruta chalepensis. The Salvia officinalis was mainly composed of b-thujone (25%), camphor (23.6%), 1,8-cineole (21.5%), viridiflorol (9.5%) and a-thujone (6%). The main constituents of Thymus vulgaris were carvacrol (48.4%), g -terpinene (14.9%), p-cymene (14.7%) and thymol (5.6%) while 2-undecanone (83.2%) constituted the major component of Ruta chalepensis essential oil. [less ▲]

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See detailInfluence of drying methods on yield and chemical composition
Dahak, K.; Wathelet, Jean-Paul ULg; Chechouani, H. et al

Poster (2009)

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See detailThe contribution of macroalgae to the assessment of the ecological quality of the rivers in Wallonia based on macrophytes indicator values in the British and French approaches.
Sossey Alaoui, Khadija ULg; Galoux, Daniel; Rosillon, Françis ULg

Poster (2009)

In accordance with the water Framework Directive (WFD, European Parliament & The Council of the European Union, 2000) defining the overall ecological status of rivers, many hundred sites were analysed in ... [more ▼]

In accordance with the water Framework Directive (WFD, European Parliament & The Council of the European Union, 2000) defining the overall ecological status of rivers, many hundred sites were analysed in the Walloon network. Within each sample, macroalgae data were gathered at species/or at genus level and the main water quality parameters were collected several times per year from 2005 to 2009. <br /><br />Seventeen species and genera cited in the French and English lists of macrophyte methods used to assess the ecological quality of rivers were considered. Within these sites, the ratio of the macroalgae among the contributory species was examined and the impact of these on the final scores of the River Macrophytes Nutrient index (United Kingdom, Wilby et al.,2006) and the biological macrophytic index in rivers (France, Haury et al., 2006) was analysed.At the same time, a ranking of these macroalgae along a trophy gradient has been established thanks to a principal component analysis of the physico-chemical parameters and a weighting of the species presence in a given waterbody by its abundance.The relationships between macroalgae scores and those found in France and in United Kingdom were studied using the Spearman rank correlation coefficient. <br /><br />Literature: <br /><br />European Parliament & The Council of the European Union, 2000. Directive 2000/60/EC of the European Parliament and of the Council establishing a framework for the Community action in the field of water policy. Official Journal of the European Communities 327: 1-72.Haury, J., M.-C. Peltre, M. Trémolières, J. Barbe, G. Thiébaut, I. Bernez, H. Daniel, P. Chatenet, G. Haan-Archipof, S. Muller, A. Dutartre, C. Laplace-Treyture, A. Cazaubon & E. Lambert-Servien, 2006. A new method to assess water trophy and organic pollution – the Macrophyte Biological Index for Rivers (IBMR): its application to different types of river and pollution. Hydrobiologia 570: 153-158. Willby, N., J. Pitt & G. Phillips, 2006. Summary of approach used in LEAFPACS for defining ecological quality of rivers and lakes using macrophyte composition. Draft Report January 2006. [less ▲]

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See detailEnrichment of Anhydrous Milk Fat in Polyunsatured Fatty Acid Residues
Aguedo, Mario ULg; Hanon, Emilien ULg; Danthine, Sabine ULg et al

Poster (2009)

Lipozyme TL IM was used in a solvent-free batch, microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ... [more ▼]

Lipozyme TL IM was used in a solvent-free batch, microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ternary blend. [less ▲]

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See detailLancement d’un programme d’amélioration génétique de Jatropha curcas L. au Sénégal
Terren, Marieke ULg; Saverys, S.; Jacquet de Haverskercke, P et al

Poster (2009)

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See detailThe Surface Ocean CO2 Atlas (SOCAT) - Coastal Regional group
Borges, Alberto ULg; Chen, A. T. C.; The SOCAT Regional group

Poster (2009)

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See detailEtude exploratoire des changements émotionnels et comportementaux chez des patients ayant la sclérose en plaques
Belhadj, Soumaya; DELRUE, Gaël ULg; VOKAER, Mathieu et al

Poster (2009)

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See detailInteresterification of rapeseed oil with anhydrous milk fat and its stearin fraction
Giet, Jean-Michel ULg; Aguedo, Mario ULg; Hanon, Emilien ULg et al

Poster (2009)

The compositional changes occurring during the lipase-catalysed interesterification of AMF/rapeseed oil (RO) and AMF stearin fraction (AMFSF)/RO blends were described in the first part of this study. In ... [more ▼]

The compositional changes occurring during the lipase-catalysed interesterification of AMF/rapeseed oil (RO) and AMF stearin fraction (AMFSF)/RO blends were described in the first part of this study. In the present and second part are reported the resulting changes in physical properties, especially the melting behaviour through solid fat content (SFC), dropping point (DP) and fusion profiles by differential scanning calorimetry (DSC). [less ▲]

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See detailLight transmission imaging as a useful tool to decrypt root water uptake
Lobet, Guillaume ULg; Javaux, M.; Draye, X. et al

Poster (2009)

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See detailInteresterification of rapeseed oil with anhydrous milk fat and its stearin fraction
Aguedo, Mario ULg; Giet, Jean-Michel ULg; Hanon, Emilien ULg et al

Poster (2009)

Rapeseed oil (RO) (a choice source of unsaturation-rich residues) was used in the present study to enrich anhydrous milk fat (AMF) with unsaturated C18 fatty acids (FA) (oleic, linoleic and linolenic ... [more ▼]

Rapeseed oil (RO) (a choice source of unsaturation-rich residues) was used in the present study to enrich anhydrous milk fat (AMF) with unsaturated C18 fatty acids (FA) (oleic, linoleic and linolenic acids). Comparatively, one “harder” fraction of AMF underwent the same reaction. The physico-chemical properties modifications induced by the reaction were followed. The compositional changes are reported in this first part and the consequent physical modifications are presented in a second part. [less ▲]

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See detailInnate lymphocytes in human papillomavirus (HPV)-associated cervical cancers
Renoux, V; Bisig, B; Thiry, Marc ULg et al

Poster (2009)

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See detailAnalysis of several analytical method validation strategies
Rozet, Eric ULg; Bouabidi, A.; Bouklouze, A. et al

Poster (2009)

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See detailA prospective randomized multicenter trial of darbepoetin-alfa and I.V. iron administration after autologous hematopoietic stem cell transplantation.
Beguin, Yves ULg; Maertens, J.; DE PRIJCK, Bernard ULg et al

Poster (2009)

We conducted a multicenter prospective randomized study analyzing the impact of darbepoetin alfa (DA) with or mithout i.v. iron on erythroid recovery after autologous HCT.

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See detailEvaluation of the endorcine disrupting chemicals contamination in the North Sea porpoise population using in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Van Der Heiden, Edwige ULg et al

Poster (2009)

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental ... [more ▼]

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental quality and posing ecological risks. Marine mammals inhabiting polluted environments accumulate high levels of these chemicals, so they can be considered good indicators of marine pollution. Thirteen major organochloride pollutants were chosen to make subject of this study: o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. All these chemicals will have their endocrine disrupting effects characterized individually and collectively by report gene expression assays. The MCF7-ERE cells used in these assays were produced in the Molecular Biology and Genetic Engineering Laboratory of the University of Liège and are originated from a human mammary carcinoma. They carry a gene expressing the synthesis of luciferase and controlled by oestrogen receptors. Then, the thirteen chemicals will be searched in the blubber tissues of porpoises by the use of gas chromatography-mass spectrometry. The blubber samples will also have their endocrine disrupting effect characterized. For the moment, the estrogenic effects of the HCH isomers were put in evidence. The next steps of this work must be soon accomplished. It is expected a great level of these compounds to be found in the samples. On the other hand, their endocrine disrupting effects and how they act in agonist, antagonist and synergist ways are subject that still needs to be cleared. [less ▲]

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See detailAgonistic sounds in the clownfish Amphiprion clarkii: implication of the swimbladder in the sound-producing mechanism
Colleye, Orphal ULg; Nakamura, Masaru; Parmentier, Eric ULg

Poster (2009)

Clownfishes are aggressive fishes that use sound production to defend their anemone territory. It has been shown that they produce agonistic sounds using a jaw teeth snapping. At present, this mechanism ... [more ▼]

Clownfishes are aggressive fishes that use sound production to defend their anemone territory. It has been shown that they produce agonistic sounds using a jaw teeth snapping. At present, this mechanism has highlighted the onset of the sound but has not explained yet which structure is responsible for the sound modulation. Interestingly, some acoustic features such as dominant frequency and pulse duration are directly related to fish size. Such variations are linked to a morphological constraint. Also, the existent relationship between fish size and swimbladder size implies that the swimbladder might be involved in the sound production. Sound analyses in Amphiprion clarkii showed that the experimental filling of the swimbladder with physiological liquid (NaCl 9‰) significantly modified the acoustic features. The most striking changes were a significant increase in dominant frequency and a significant decrease in pulse duration. These observations highlighted the implication of the swimbladder in sound modulation. In clownfishes, dominant frequency and pulse duration are morphologically determined signals. The swimbladder appears to modulate these acoustic features by acting as a resonant chamber. [less ▲]

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See detailTotal Error-Based Criterion for Analytical Method Transfer
Rozet, Eric ULg; Dewe, W.; Boulanger, Bruno ULg et al

Poster (2009)

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See detailMapping of soil-sediment systems contaminations around a metal-ore smelter : The example of Cu in Lubumbashi (R.D.Congo).
Mpundu, Michel; Mukobo, Robert-Prince; Kaya Muyumba, Donato ULg et al

Poster (2009)

The Katanga is famous for its high richness in metal ores, mainly Cu- and Co-minerals. The ore treatments activities lead to metal spreading in the environment which do endanger the viability of ... [more ▼]

The Katanga is famous for its high richness in metal ores, mainly Cu- and Co-minerals. The ore treatments activities lead to metal spreading in the environment which do endanger the viability of ecosystems and human health. The contaminations of soils and sediments around the Gecamines smelter in Lubumbashi have been evaluated through a multi-scale approach. In the first stage, a reference system about the natural contents in soils has been implemented. The main units of the detailed (1:20 000) soil map have been characterized through field observations and laboratory determinations : acido-basic and organic status, particle size distribution, cationic exchange capacity, total contents in Al, Fe, Mn, Cu, Co, Cd, Pb, Zn. An exploratory mapping of soil contaminations (Cu, Co, Cd, Pb, Zn) centered on the Gecamines cheminey was then conducted as a second stage of the approach. Detailed investigations were finally performed in the Gecamines district where signs of soil degradation were the highest. The spatial continuity and the differenciation (C : C0+C ratio) of the studied parameters appeared weak. No clear effect of the dominant wind direction on the spatial distribution of the contaminants could be identified despite a clear degradation of the ecosystem in the area under dominant wind. However the detailed approach showed evidences of (i) significant wind deposits in the Gecamines district, (ii) and local man-made deposits but also (ii) important processes of redistribution in the landscape through erosion/sedimentation or anthropic activities. Our results point out the difficulty to identify the sources of contaminations in an urban environment and stress the need to have approaches with complementary levels of details. [less ▲]

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See detailEvaluation of decision methodologies for analytical method validation
Rozet, Eric ULg; Rudaz, S.; Bouabidi, A. et al

Poster (2009)

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See detailSynthesis of Ag/TiO2 hybrid photocatalysts by sol-gel process with new P-alkoxide functionalized ligands
Lambert, Stéphanie ULg; Bied, Catherine; Bodson, Céline et al

Poster (2009)

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See detailTransmission des prix dans le secteur laitier en France
Burny, Philippe ULg

Poster (2009)

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See detailWheat root ecophysiology: understanding communication between wheat root system and soil microorganisms.
Delaplace, Pierre ULg; d'Ans, Séverine ULg; Wathelet, Jean-Paul ULg et al

Poster (2009)

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up ... [more ▼]

Studies leading to a better understanding of the mechanisms involved in the communication between soil microorganisms and root system can potentially lead to improved fertility management strategies. Up to now, the characterization of such interactions has been mainly focused on root exsudates but volatile organic compounds (VOC) also play a role as chemical messengers in positive interactions occurring in the rhizosphere. We aim to better understand the VOC-mediated chemical ecophysiology of soil. Wheat (Triticum aestivum L.) was chosen in this project based on its importance for food production and industrial applications. [less ▲]

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See detailSet-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailThe effects of purified Z- and E-guggulsterones from guggul-gum extract on human preadipocytes proliferation and differentiation through the study of gene expression of adipose markers.
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailProduction and toxicity of γ-decalactone and 4-hydroxydecanoic acid from Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The ... [more ▼]

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The effect of gum tragacanth on the production of γ-decalactone by R. aurantiaca was evaluated. [less ▲]

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See detailHigh-throughput methodology applied to screening of carbohydrate-based surfactant interfacial properties for colloidal system formulations
Razafindralambo, Hary ULg; Blecker, Christophe ULg; Deroanne et al

Poster (2009)

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and ... [more ▼]

Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and non-food applications. Based on many functional groups of the carbohydrate part, it is possible to design multiple amphiphilic structures of CBS varying in the hydrophilic head groups (mono-, oligo-, or polysaccharides), hydrophobic tail (mono-, di-, tricatenar) but also in the linker/spacer between them. The main structures of CBS include mono- and bicatenar glycolipids, bolaforms, and gemini. Moreover, CBS compounds can be produced from the most abundant renewable materials allowing large product concept possibilities. Among general properties of surfactants, interfacial properties molecules occupy a fundamental key role for colloidal system formulations since they control most of technological aptitudes required for forming and stabilizing food, cosmetic, agrochemical, detergent, and pharmaceutical products. High-throughput methodology applied to screening of CBS interfacial properties appears crucial for achieving optimum formulations of colloidal systems like foams and emulsions for which small amounts of single or mix surface-active agents are often needed and numerous physical and chemical parameters are involved. This general approach may be applied to any amphiphilic molecules produced from other renewable resources of surface-active compounds like micro-organisms and by direct extraction from vegetable materials. One of the key factors for the development of this methodology is the use of automated instrument systems in laboratory scale which are still lacking in this field compared to those existing in the analytical chemistry and biochemistry areas. [less ▲]

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See detailEvaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts
Grenade, Charlotte ULg; Borget, Pascal; Moniotte, Nicolas et al

Poster (2009)

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The ... [more ▼]

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes. After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF). Materials and methods The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage. Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen. A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture. First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane. For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium. Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm). After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect. Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy. Conclusion Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers. In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers. [less ▲]

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See detailReintroduction & effects on their environment of indigenous plants of the Cerrado
Buisson, Elise; Le Stradic, Soizig ULg; Negreiros, Daniel et al

Poster (2009)

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See detailThe strange case of the Of?p stars
Nazé, Yaël ULg; Walborn, N. R.; Martins, F. et al

Poster (2009)

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See detailClinal differentiation during invasion: Senecio inaequidens (Asteraceae) along altitudinal gradients in Europe.
Monty, Arnaud ULg; Mahy, Grégory ULg

Poster (2009)

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic ... [more ▼]

Plant population differentiation may play a role in decreasing the ability to predict whether, where, and when an introduced species will invade. However, few studies have addressed the level of genetic change an alien species may undergo during range expansion, e.g. in response to climatic variation with altitude. In the present study, we tested the hypothesis that invasive populations of Senecio inaequidens (Asteraceae) differentiated during migration from two independent introduction sites into divergent altitudinal and climatic zones. We carried out two years of common garden experiments with eight populations from a Belgian altitudinal transect and ten populations from similar French transect. Climatic analysis revealed that the Belgian transect followed a temperature and precipitation gradient. A temperature and summer drought gradient characterized the French study site. We evaluated differentiation and clinal variation in the following characters: days to germination, days to flowering, height at maturity, final plant height and aboveground biomass. Results showed that S. inaequidens populations differentiated in growth traits during invasion. First year of experiment, regressing population growth trait means against source population altitude indicated the presence of clinal variation along both transects. Second year, similar results were found along the French transect, i.e. a reduction in height at maturity, plant height and aboveground biomass with increasing altitude. Including seed mass as a covariate did not change the outcome of the analysis. The possible evolutionary causes for the differentiation observed are discussed. [less ▲]

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See detailInvasive plant species management tests in Southern Belgium
Delbart, Emmanuel ULg; Halford, Mathieu ULg; Pieret, Nora et al

Poster (2009)

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See detailGenetic structure of Barn swallow (Hirundo rustica).
Dardenne, Sophie ULg; Stevens, Virginie ULg; Hollander, F. et al

Poster (2009)

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See detailEvaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Remacle, Anne-Sophie ULg et al

Poster (2009)

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be ... [more ▼]

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be very slowly degraded, decreasing the environmental quality and causing ecological risks. Marine mammals inhabiting polluted environments accumulate high quantities of these chemicals, and are good indicators of marine pollution Thirteen major organochloride pollutants (known from literature to contaminate north sea porpoise) were chosen in this study – o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. The thirteen chemicals were tested individually and in mixtures by the use of report gene expression assays. The MCF7-ERE cells used in the assays were originated from MCF7 human mammary tumor cells transfected with the ERE-luciferase reporter system. Preliminary results suggest that o,p’-DDD; p,p’-DDD; o,p’-DDT; HCB; β-HCH; δ-HCH; PCB 138 and PCB 180 are able to stimulate the luciferase expression of MCF7-ERE cells when they are present individually or in mixtures. No synergism was observed within mixtures. When MCF7-ERE cells were exposed to the chemicals and 17β-oestradiol simultaneously, p,p’-DDE; α-HCH; PCB 138 and PCB 180 could raise the luciferase expression in comparison to the exposition of 17β-oestradiol alone; on the other hand, o,p’-DDD; p,p’-DDT; HCB; β-HCH; γ-HCH and δ-HCH seem to inhibit the luciferase expression at low doses. In the next step, the pollutants will be searched and quantified in the blubber tissue of porpoises by use of gas chromatography-mass spectrometry. The blubber tissue samples will also have their endocrine disrupting effects characterized. This work is ongoing and It is expected a relevant level of these compounds to be found in the samples. Conversely, their endocrine disrupting effects and how they act in agonist, antagonist ways are subject that still needs to be studied. [less ▲]

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See detailThe fast regulation of aromatase activity by phosphorylations is species and tissue-independent.
Charlier, Thierry ULg; Harada, Nobuhiro; Ball, Gregory F. et al

Poster (2009)

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See detailGlass production in late antiquity
Van Wersch, Line ULg; Mathis, François ULg; Dupuis, Thomas ULg et al

Poster (2009)

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

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See detailAchromatization of solar concentrator using diffractive optics
Languy, Fabian ULg; Lenaerts, Cedric ULg; Loicq, Jerôme ULg et al

Poster (2009)

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux ... [more ▼]

Photovoltaic energy suffers from payback time. High solar concentration (above 500 suns) is a very interesting way to reduce production cost. The use of lenses allows more flexibility to make light flux more uniform. In addition lenses are less prone to errors of manufacture and can be easily duplicated. Unfortunately, optical materials are very dependent on the wavelength which causes important chromatic aberrations. One can think about achromatic doublets but doublets are thick, heavy and require exotic glasses which make them expensive. During the conference, I would like to present an innovative way to achromatize solar concentrators using hybrid (refractive/diffractive) lenses. Indeed, an hybrid lens is as thin as a Fresnel lens and is made in only one glass. But diffractive lenses suffer from limited broadband diffraction efficiency due to spurious orders. To overcome the lake of efficiency we are investigating the use of multi-layer diffractive optical element. This technique allows to achieve an efficiency greater than 97% over the full visible spectrum using only three components (e.g. two optical elements + air). [less ▲]

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See detailCarbon cycling in the mixolimnion of Lake Kivu (East Africa)
Borges, Alberto ULg; Descy, J.-P.; Delille, Bruno ULg et al

Poster (2009)

Detailed reference viewed: 17 (4 ULg)