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See detailModel of the Jovian magnetic field topology constrained by the Io auroral emissions
Hess, S.; Bonfond, Bertrand ULg; Grodent, Denis ULg et al

Poster (2011, July 11)

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See detailEvaluating radical conservation futures: strategies for assessing the potential for Eurasian lynx in the Benelux countries
Bouyer, Yaëlle ULg; Poncin, Pascal ULg; Beudels-Jamar, Roseline et al

Poster (2011, July 08)

Tolerance of anthropic environment has allowed large carnivores to recolonize and to be reintroduced within Europe in the last forty years. This indicates that, even in areas where the return of large ... [more ▼]

Tolerance of anthropic environment has allowed large carnivores to recolonize and to be reintroduced within Europe in the last forty years. This indicates that, even in areas where the return of large carnivores seems unlikely, their presence and establishment is possible, even in highly modified environments. In the current context of the return of large carnivores in Europe, there is a need for scientific conservation tools to assess the full potential and consequences of the return of large carnivores in Europe, and in particular to evaluate potential impact on game and livestock. Today, the return of the Eurasian Lynx (Lynx lynx) in areas where it has been absent for long periods is becoming increasingly probable, and this requires further investigations. Three main lines of research will be particularly important: the use of habitat by Eurasian Lynx, the effect of habitat on predation behavior, and potential sources of conflict with human populations. The research project will address fine scale habitat use, the effect of prey‘s spatial variation on Lynx predation behavior, the use of different statistical methods to estimate Lynx‘s viability in various habitats and landscape, and a review of current conflicts throughout Europe. Results should allow better understanding of the Lynx redeployment potentials in lowland Western Europe [less ▲]

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See detailProduction de cartes généralisées à partir de la Carte Numérique des Sols de Wallonie
Legrain, Xavier ULg; Demarcin, Pierre ULg; Mokadem, Abdel Illah et al

Poster (2011, July 07)

La Belgique est parmi les premiers et rares pays à s’être dotée, entre 1947 et 1991, d’une carte des sols à grande échelle (1/20 000) sur l’ensemble de son territoire. Entretemps, le développement actuel ... [more ▼]

La Belgique est parmi les premiers et rares pays à s’être dotée, entre 1947 et 1991, d’une carte des sols à grande échelle (1/20 000) sur l’ensemble de son territoire. Entretemps, le développement actuel des applications numériques d’une part, une prise de conscience accrue de l’importance de préserver les sols d’autre part, ont amené la Région wallonne à entreprendre la digitalisation de la partie wallonne de la Carte des Sols de la Belgique, pour aboutir à la production d’une couche numérique cohérente sur l’ensemble du territoire wallon, accompagnée d’une légende unique et exhaustive : la Carte Numérique des Sols de Wallonie (CNSW). Sa très grande précision, tant spatiale que sémantique, en fait un outil d’analyse puissant, mais également particulièrement complexe à manipuler. Partant du constat qu’une grande partie du territoire peut être couverte à partir d’un nombre limité d’unités cartographiques parmi les 6 000 existantes dans la CNSW, une carte des Principaux Types de Sols de Wallonie à l’échelle du 1/250 000 en a été dérivée, offrant ainsi une vision d’ensemble et une perspective d’intégration européenne. La légende fut conçue à partir de regroupements logiques des sols sur base de trois des quatre critères majeurs des unités cartographiques de la CNSW, à savoir la texture, le drainage naturel et la nature de la charge caillouteuse lorsqu’elle est présente. Adoptant des principes de dominance par l’utilisation des termes « principalement » ou « quasi-exclusivement », elle ne peut néanmoins être qualifiée de carte d’associations de sols du fait des restrictions de regroupement adoptées. Outre le rattachement de chaque plage cartographique à une des classes ainsi définies, un processus de généralisation cartographique a été effectué dans le but d’une meilleure lisibilité à l’échelle du 1/250 000. Cette carte fut utilisée comme support à la sectorisation du territoire wallon en 24 Districts de l’Espace Rural, sur base d’une analyse à caractère géomorphopédologique. Ces Districts sont à leur tour utilisés pour la réalisation de cartes des Principaux Types de Sols à l’échelle du 1/100 000, sur le même principe que la carte à l’échelle du 1/250 000. Ces diverses cartes généralisées permettent d’envisager la confrontation de la CNSW avec plusieurs gisements de données d’analyse disponibles, selon différentes échelles. En tant qu’outil d’intégration, de structuration et de cadrage de l’information pédologique, elles doivent devenir de plus en plus le fondement de Systèmes Régionaux d’Information sur les Sols et leurs propriétés. [less ▲]

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See detailInfluence of thermoelectric coupling on pacemaker activity generated by mechano-electric feedback in a one-dimensional ring-shaped model of cardiac fiber
Collet, Arnaud ULg; Desaive, Thomas ULg; Pierard, Luc ULg et al

Poster (2011, July 05)

Recently, the influence of thermal processes on electrophysiology has clearly been underlined, using a FitzHugh–Nagumo-type (FHN-type) model. When the temperature is raised, the action potential duration ... [more ▼]

Recently, the influence of thermal processes on electrophysiology has clearly been underlined, using a FitzHugh–Nagumo-type (FHN-type) model. When the temperature is raised, the action potential duration (APD) has been shown to shorten, while the action potential (AP) amplitude decreases, and the conduction velocity increases. In the present study, we investigate the effects of thermoelectric coupling on mechano-electric feedback (MEF), and more specifically, on pacemaker activity generated by MEF. To investigate these effects, thermoelectric coupling is introduced in a one-dimensional ring-shaped electromechanical model of cardiac fiber, which takes into account excitation-contraction coupling (ECC), as well as MEF. [less ▲]

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See detailEvaluation of the pedogenesis effects on the fractionation of metallic trace elements in solid wastes from industrial activities
Pineux, Nathalie ULg; Liénard, Amandine ULg; Cloots, Rudi ULg et al

Poster (2011, July 04)

This work aims at contributing to a better knowledge of the reactivity of metal-bearing phases to evaluate the risks of metal trace elements (MTE) transfers in soil-plant-water system. The high proportion ... [more ▼]

This work aims at contributing to a better knowledge of the reactivity of metal-bearing phases to evaluate the risks of metal trace elements (MTE) transfers in soil-plant-water system. The high proportion of the MTE on this type of sites seems to be mobilized only by particularly aggressive conditions but unstable bearing mineral phases have also been observed in smaller proportions. [less ▲]

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See detailA Methodology for Sampling Design dedicated to the Evaluation of Effects of Soil Type and Land Use on the Fate of Metallic Trace Elements in Contaminated Soils
Liénard, Amandine ULg; Bock, Laurent ULg; Colinet, Gilles ULg

Poster (2011, July 04)

Contaminated soils may present a risk to human health and the environment. The aim of this study is to evaluate the fate of metallic trace elements (Cd, Pb and Zn) brought to soils from atmospheric ... [more ▼]

Contaminated soils may present a risk to human health and the environment. The aim of this study is to evaluate the fate of metallic trace elements (Cd, Pb and Zn) brought to soils from atmospheric fallouts according to soil type and land use. We have developed a sampling soil strategy in order to both map soil contaminations and assess the effects of soils and land use on the mobility of MTE. [less ▲]

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See detailSimulating the Holocene re-colonization of Europe by tree species using dynamic vegetation models
Dury, Marie ULg; Lehsten, Dörte; Warnant, Pierre et al

Poster (2011, July)

Pollen and macro-fossil data collected from various localities in Europe provide the opportunity to reconstruct the speed and the routes of the post-glacial spread of European tree species. Moving from a ... [more ▼]

Pollen and macro-fossil data collected from various localities in Europe provide the opportunity to reconstruct the speed and the routes of the post-glacial spread of European tree species. Moving from a limited number of refugia at the end of the glacial period, tree species have progressively re-colonized the continent through the Holocene at seemingly species-specific migration rates. However, the relative roles of climatic fluctuations, dispersal capacities of individual species, and inter-specific competition in controlling these rates remains controversial. Here, we investigate these different aspects with two dynamic vegetation models (DVM), LPJ-GUESS and CARAIB. Transient runs of both models were performed over the Holocene, using HadCM3 GCM-reconstructed climate. Large-scale species migration at 0.5°x0.5° is represented in these models using migration rates derived from a small-scale cellular automaton, CATS. Individual tree species migration rates were pre-calculated with CATS every 1000 years over each grid cell used by the DVMs in the climatic conditions reconstructed by the GCM. In the DVMs, these migration speeds were influenced by the response to competition from other species. The DVMs were used to study the migration of one species, from its 10 kyr BP refugia, within a landscape defined by a set of other species for which no dispersal limitations are assumed. Here, we illustrate the results obtained for three wind-dispersed tree species: Abies alba, Picea abies, Fagus sylvatica and compare them to their past distributions reconstructed from pollen and macro-fossil data. [less ▲]

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See detailPhonological representations in children with SLI : a study of French vowels.
Maillart, Christelle ULg; Cazeneuve, Julia; MacLeod, Andrea

Poster (2011, July)

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See detailConscious and Unconscious Reward Cues Can Affect a Critical Component of Executive Control: (Un)conscious updating?
Bustin, Gaëlle ULg; Capa, Rémi ULg; Cleermans, Axel et al

Poster (2011, July)

The present study investigates whether updating an important function of executive control can be driven by unconscious reward cues. Participants had to memorize several numbers and update those numbers ... [more ▼]

The present study investigates whether updating an important function of executive control can be driven by unconscious reward cues. Participants had to memorize several numbers and update those numbers independently according to a sequence of arithmetic operations. At the beginning of each trial, a reward (1 euro or 5 cents) was presented, either subliminally or supraliminally. Participants could earn the reward if they found the correct response on the updating task. Results showed better performance when a high (conscious or unconscious) reward was at stake compared to a low reward. This suggests that subliminal information can influence a component process of executive control traditionally thought to require consciousness. [less ▲]

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See detailIncidental non linguistic regularities learning in Children with
Gabriel, Audrey ULg; Meulemans, Thierry ULg; Parisse, Christophe et al

Poster (2011, July)

Recent studies on specific language impairment (SLI) have suggested that language deficits could be partly explained by the Procedural Deficit hypothesis (PDH; Ullman & Pierpont, 2005). Tomblin et al ... [more ▼]

Recent studies on specific language impairment (SLI) have suggested that language deficits could be partly explained by the Procedural Deficit hypothesis (PDH; Ullman & Pierpont, 2005). Tomblin et al. (2007) and Lum et al. (2009; 2011) obtained data supporting this interpretation with the serial reaction time (SRT) task, as well as Evans et al. (2009) and Plante et al. (2002) with artificial grammar tasks. Recently, Gabriel et al. (2011) obtained contrasting results, showing that children with SLI were able to detect non linguistic regularities during a SRT task. The aim of this study was to assess the PDH by using a non-linguistic artificial grammar learning tasks in order to mimic real conditions of language acquisition. Twenty-three children with SLI and their typically developing (TD) peers are compared on a task in which the incidental learning sequence was presented through visual shapes via a laptop.These results confirm our previous study (Gabriel et al., 2011) by showing that children with SLI detect the rules in non-linguistic conditions. • So, contrary to results of previous studies (Evans et al., 2009; Lum et al., 2009; 2011; Plante et al., 2002; Tomblin et al., 2007), this study does not confirm the PDH in children with SLI, or at least suggests that, if present, the deficit of the procedural system in SLI is not going beyond the language system. [less ▲]

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See detailEffects of long term soil organic matter restitution mode on soil heterotrophic respiration and soil biological properties.
Buysse, Pauline ULg; Carnol, Monique ULg; Malchair, Sandrine ULg et al

Poster (2011, July)

Soil heterotrophic respiration (SHR) is the process by which CO2 is released during organic matter decomposition. It is generally expected that SHR can act as a positive feedback to global warming ... [more ▼]

Soil heterotrophic respiration (SHR) is the process by which CO2 is released during organic matter decomposition. It is generally expected that SHR can act as a positive feedback to global warming, therefore leading to more CO2 release into the atmosphere. It is thus important to better understand this process. Particularly, agricultural soils may behave as important CO2 sources that are strongly influenced by soil and crop management (e.g. organic matter restitution modes, hereafter “OM-RM”). The present study aimed at determining if, after more than 50 years of application of different OM-RM, (1) significant differences of SHR fluxes can be observed between treatments, (2) SHR responses to temperature and soil moisture content can be affected by the OM-RM and (3) the experimental design is suitable to assess potential differences between treatments. The experimental field is situated in Liroux, near Gembloux in Belgium. At that site, a long term experiment with different OM-RM runs from 1959 onwards. For the present study, three contrasted treatments were considered: (1) exportation of all residues after harvest, (2) addition of manure once every three to four years and (3) restitution of residues after harvest. SHR flux measurements were carried out manually on fourteen occasions from 2 April to 30 July 2010, using a dynamic closed chamber system. Temperature and soil moisture content at 5 cm depth were also measured manually. Results showed that after more than 50 years of OM-RM application, no significant differences could be observed between the three treatments in terms of SHR fluxes and SHR responses to temperature or soil moisture, while the soil organic carbon content did vary significantly between them. The sensitivity to temperature was quite low in all treatments, with a mean Q10 value of 1,36. Besides, SHR fluxes were seen to be more responsive to increases in soil water content than to absolute soil moisture content values. Indeed, when soil moisture content increased between two consecutive measurement dates, the ratio of the corresponding SHR fluxes was larger than 1. Particularly dry conditions in 2010 may actually have caused the fluxes to be very low, making the assessment of differences between treatments more difficult. Moreover, soil dryness is likely to be responsible for the SHR flux increases after rain events, as caused by re-solubilization of organic compounds. Also, an important spatial variability was observed, which may have obscured the assessment of potential differences between treatments. Further investigations will consist in performing a new flux measurement campaign in 2011 that will take the spatial variability issue into account, and in monitoring microbial and soil properties in the different treatments, such as microbial biomass, metabolic activity and labile carbon. [less ▲]

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See detailDigital Collaborative Studio : 4 years of practice
Safin, Stéphane ULg; Kubicki, Sylvain; Bignon, Jean-Claude et al

Poster (2011, July)

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See detailReproducibility and repeatability of upper limb landmarks palpation for junior operators
Schwartz, Cédric ULg; Fedrigo, Tatiana ULg; Bruls, Olivier ULg et al

Poster (2011, July)

In human motion analysis, bone motions are usually expressed relatively to anatomical reference frames. The anatomical reference frames are constructed thanks to the localization of bony landmarks during ... [more ▼]

In human motion analysis, bone motions are usually expressed relatively to anatomical reference frames. The anatomical reference frames are constructed thanks to the localization of bony landmarks during a static phase prior to the acquisitions. These landmarks are identified by means of palpation. Accurate comparison between subjects and studies implies good reproducibility and repeatability of the palpation process. However, all investigators don’t have a long experience in palpation. In this paper, the reproducibility and repeatability of palpation for junior investigators were measured. Results show worse reproducibility and repeatability than what is usually expected. These errors have particularly an influence on the definition of the reference frames of the arm. This study therefore emphasizes on the need of a specific training of operators working in a motion lab. [less ▲]

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See detailInvestigation on the Role of the Viral Semaphorin encoded by the A3 Gene of Alcelaphine Herpesvirus 1 in the Induction of Malignant Catarrhal Fever
Myster, Françoise ULg; Palmeira, Leonor ULg; Vanderplasschen, Alain ULg et al

Poster (2011, July)

Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried by wildebeest asymptomatically. AlHV-1 is however responsible for the development of malignant catarrhal fever (MCF) when cross-species ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried by wildebeest asymptomatically. AlHV-1 is however responsible for the development of malignant catarrhal fever (MCF) when cross-species transmitted to a variety of ruminant susceptible species. Wildebeest-derived (WD)-MCF is a fatal lymphoproliferative and degenerative disease of ruminants. Experimentally, WD-MCF can be reproduced in rabbits. A3 ORF encodes a putative semaphorin homolog protein, named AlHV-sema. Semaphorins are secreted and membrane-associated proteins characterized by a conserved ‘Sema’ domain. Initially identified as guidance factors assisting axons pathfinding during neural development, semaphorins have been shown over the last decade to have significant functions in various processes of immunoregulation. Phylogenetic analyses revealed that AlHV-sema and mammals Sema7A have a common ancestor and that AlHV-Sema has evolved independently of other viral semaphorins. Further bioinformatics analyses demonstrated that AlHV-Sema and cellular Sema7A share a highly similar tridimensional structure. In order to investigate the role of AlHV-Sema in WD-MCF induction, we used the AlHV-1 BAC clone and produced a strain deleted for A3 and a revertant strain. The strain deleted for A3 replicated comparably to the wild-type parental strain in vitro. In vivo, rabbits infected with the strain deleted for A3 developed WD-MCF with a small but significant delay compared to those infected with the parental and revertant strains. Deletion of A3 did not affect the increase of viral genomic charge over time in peripheral blood and in lymph nodes at time of death and the major histopathological lesions were present in all groups. Though infection with wild-type and revertant strains resulted in the inversion of CD8 over CD4 ratio and increased IFN- production in lymphoid tissues at time of death, both parameters were significantly reduced after infection with the A3 deleted strain. Together, these results suggest that AlHV-Sema play a role in the host response to AlHV-1 infection. [less ▲]

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See detailDo we need to standardize extraction procedures for community level physiological profiling?
Carnol, Monique ULg; Bosman, Bernard ULg; Malchair, Sandrine ULg

Poster (2011, July)

Microorganisms are essential regulators of soil functioning, as they are involved in many crucial processes such as organic matter decomposition, nutrient cycling, soil structure and fertility. Currently ... [more ▼]

Microorganisms are essential regulators of soil functioning, as they are involved in many crucial processes such as organic matter decomposition, nutrient cycling, soil structure and fertility. Currently, there is a growing interest in functional diversity, such as the number and type of substrates used for energy metabolism (CLPP-community level physiological profiling). Such metabolic diversity of heterotroph soil bacteria is frequently investigated through Biolog Ecoplates, containing 31 of the most useful carbon sources for the soil community. The metabolic diversity of soil bacteria might be an interesting biological indicator of soil quality, and also a useful tool for investigating the link between land use change, climate warming, soil carbon, microbial diversity and activity. Methods related to Biolog-CLPP reported in the literature differ in the suspension medium and extraction method, the type and density of inoculums, the inoculation procedures and conditions of incubations. For example, various combinations of extraction methods and suspension media are being used for the first bacterial extraction step. Despite such methodological differences, Biolog-CLPP data are often compared across studies. The development of a standardised method for Biolog-CLPP is however essential improving the relevance and significance of results across studies. In this work, we investigated the influence of extraction procedures on microbial extraction efficiency for further use in CLPP. The microbial extraction efficiency was tested by plate counts for a total of twelve combinations of three suspension media and four extraction methods. The experiment was performed on four soils differing in organic matter content. The aims of this study were to: • Synthesize extraction procedures used for Biolog-CLPP • Measure the effect of extraction procedures on microbial extraction efficiency (plate counts) in four soil types • Investigate a possible interaction between the suspension media and the extraction method used • Evaluate whether a standardized extraction procedure can be recommended across soil types [less ▲]

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See detailStress negative Piétrain boars and their hybrids - Growth performances and semen quality
Do Duc, Luc ULg; Ha Xuan, Bo; Farnir, Frédéric ULg et al

Poster (2011, July)

A total of 15 boars from 3 genetic groups were used for this study, including 5 Piétrain boars (purebred Piétrain), 5 ♀Duroc × ♂Piétrain (½ Piétrain) and 5 ♀(Piétrain × Duroc) × ♂Duroc (¼ Piétrain) in ... [more ▼]

A total of 15 boars from 3 genetic groups were used for this study, including 5 Piétrain boars (purebred Piétrain), 5 ♀Duroc × ♂Piétrain (½ Piétrain) and 5 ♀(Piétrain × Duroc) × ♂Duroc (¼ Piétrain) in order to evaluate the growth performance and sperm quality of stress negative Piétrain boars and their hybrids. Testing period started at an average age of 60 days and ended at an average age of 225 days. The growth performances were weights at starting and finishing periods, average daily gain, backfat thickness, longissimus depth and lean content. The sperm quality was assessed using ejaculate volume, spermatozoon motility, sperm concentration, total number of spermatozoon in the ejaculate, rate of abnormal spermatozoon and pH of sperm. All these measurements were used to compare the genetic groups. The results showed that: The average daily gain of stress negative Piétrain boar purebred was not significantly different from Piétrain hybrids while the lean content was higher. The ejaculate volume and the spermatozoon motility of stress negative Piétrain purebred boars were higher than Piétrain hybrids but the sperm concentration and the total number of spermatozoon in the ejaculate were lower. [less ▲]

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See detailTime trends of blood leucocytes, neutrophils and plasmatic myeloperoxidase in the perioperative period of horses undergoing colic surgery.
Salciccia, Alexandra ULg; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

Poster (2011, July)

Background: Despite the recent advances in this area, colic remains a major cause of morbidity and death in horses. Neutrophilic activation and degranulation may play a key role in the postoperative ... [more ▼]

Background: Despite the recent advances in this area, colic remains a major cause of morbidity and death in horses. Neutrophilic activation and degranulation may play a key role in the postoperative complications. Activated neutrophils release enzymes like proteases and myeloperoxidase (MPO). MPO concentrations in plasma and tissue are considered as a marker of neutrophil activation. (McConnico et al. 1999; Hoy et al. 2002). When freed in the tissue, active MPO is able to oxidize, nitrate and chlorate most organic molecules (Klebanoff 2005). Objectives: The aim of this study was 1) to determine the time trends of blood leukocyte and neutrophil counts as well as of plasmatic MPO concentrations in the perioperative period of horses undergoing colic surgery and 2) to relate these time trends to the location of the pathology, the severity of postoperative complications and to the outcome of the patients. Methods: Fifty two horses undergoing colic surgery at the Equine Teaching Hospital of the University of Liege were included in this study. The location of the predominant lesion of the intestine, the severity of the postoperative complications and the outcome were recorded for each horse. Total leukocyte and neutrophil counts were performed in all of the horses while plasmatic myeloperoxidase levels were determined in 16 of them. The blood samplings were realized before and during the surgery (after correction of the intestinal lesion), during the recovery and every 4 hours during the first 4 days (from day 0 until day 4) and then every 12 hours until day 6 (150th hour after the first blood sampling) or until euthanasia. Hematologic analyses were performed at the time of sampling by use of the Medonic CA 530 (Menarini, Zaventem, Belgium). The blood was then centrifuged and the plasma was aliquoted and frozen at -20° C until assayed. MPO was assayed with a specific enzyme-linked immunosorbent assay (Equine MPO-ELISA kit, BiopTis, Liège, Belgium). A mixed model was used to analyze the time trends of leukocytes, neutrophils and MPO. All computations were done with the SAS (Statistical Analysis System) procedure Proc Mixed, with Satterthwaite degrees of freedom. The significance level was set at p=0.05. Results: The main pathology was found in the large intestine in 30 horses (58%) and the small intestine in 22 horses (42%). Forty horses (77%) survived to discharge from the clinic. Twelve horses were euthanized during the postoperative period. Their survival time varied from 0.5 day to 20 days with a mean of 7.8 days. Twenty-two horses (42%) showed none or mild complications, 12 horses (23%) showed moderate complications and 18 horses (35%) suffered from severe complications. Time trends for leukocytes and neutrophils were similar to each other (p= 0.7205) and significantly different (p< 0.0001) from the MPO time trend, which increased during the first hours, while the neutrophil time trend decreased immediately after the admission. The time trend of neutrophils was higher in large intestinal than in small intestinal pathologies and the time trend of MPO was lower in large intestinal than in small intestinal pathologies. The time trends of neutrophils were significantly different between the degrees of complications (no/mild vs moderate vs severe). For the first part of the curve, the more severe the complication, the lower is the time trend. The time trend of MPO was lower in survivors. The time trend of neutrophils in survivors was higher during the first 4 days thereafter it becomes lower than in non survivors. Conclusions: These results confirm that neutrophil counts and MPO levels undergo timely changes and that they are related to the severity of the inflammatory reaction in surgical colic cases. Knowing the kinetics of these parameters is an essential step to further determine cut-off values (with a larger group of horses) for the prognosis of horses after colic surgery. References Hoy, A., Leininger-Muller, B., Kutter, D., Siest, G. and Visvikis, S. (2002) Growing significance of myeloperoxidase in non-infectious diseases. Clin. Chem. Lab. Med. 40, 2-8. Klebanoff, S.J. (2005) Myeloperoxidase: friend and foe. J. Leukoc. Biol. 77, 598-625. McConnico, R.S., Weinstock, D., Poston, M.E. and Roberts, M.C. (1999) Myeloperoxidase activity of the large intestine in an equine model of acute colitis. Am. J. Vet. Res. 60, 807-813. [less ▲]

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See detailEvaluation of the effect of general anaesthesia on ultrasonographic images of the small intestine in horses.
Salciccia, Alexandra ULg; Gougnard, Alexandra ULg; de la Rebière de Pouyade, Geoffroy ULg et al

Poster (2011, July)

ultrasonography is currently more and more used in equine acute abdominal disease as well as in the follow-up of surgical colic patients because of its sensibility for the detection of small intestinal ... [more ▼]

ultrasonography is currently more and more used in equine acute abdominal disease as well as in the follow-up of surgical colic patients because of its sensibility for the detection of small intestinal distension. General anaesthesia is known to diminish gastrointestinal motility even if there are no clinical signs associated with it. The aim of this study was to evaluate the effect of general anaesthesia on transabdominal ultrasonographic images of the small intestine in horses undergoing routine non abdominal surgeries. The ultrasonographic exams were performed in 19 horses before and after the general anaesthesia (immediately after the recovery and then 2h, 12h and 24h after it). Each ultrasonographic exam focused on the duodenum, the jejunum and the presence of peritoneal fluid. For the jejunum, 5 locations were used: on the ventral midline just caudally to the sternum, on the left and right cranial ventral parts of the abdomen, on the left and right inguinal regions. Other parameters such as the gut sounds and the postoperative fecal output were also recorded. Anova and Chi-square tests were used for the statistical analysis. No horse showed colic signs. No significant difference was found between the pre and post anaesthetic period considering the maximal diameter of the duodenum, the maximal diameter of the jejunum on the 5 locations and the peritoneal fluid. The contractions of the duodenum were increased at the recovery compared to before the anaesthesia (p= 0,0299). The small intestine was most visible at the recovery (57,5%) and then at 2 hours after it (38,3%). It seemed that the ventral midline just caudally to the sternum and the right and left inguinal regions were the best locations to observe the jejunum (with a mean of respectively 44,6%, 39,8% and 38,5% of visualization of the jejunum).The gut sounds were very significantly decreased at the recovery (p < 0,0001) and at 2h after it (p = 0,0006). The postoperative fecal output was not decreased. In conclusion, even if general anaesthesia seems to reduce temporarily the intestinal activity (decreased gut sounds in the early post anaesthetic period), it does not cause significant distension of the small intestine (almost the small intestinal diameters were in the normal range). If an increased diameter of small intestine is observed by ultrasonography after surgery it should therefore be attributed to a pathological process and not to the anaesthesia. [less ▲]

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See detailWhen morphometrics meet genetics: the case of the common dormouse, Muscardinus avellanarius
Mouton, Alice ULg; Renaud, Sabrina; Michaux, Johan ULg

Poster (2011, July)

The aim of the study is to apply geometric morphometrics to the study of evolutionary processes that might have driven intra-specific differentiation of the common dormouse Muscardinus avellanarius in ... [more ▼]

The aim of the study is to apply geometric morphometrics to the study of evolutionary processes that might have driven intra-specific differentiation of the common dormouse Muscardinus avellanarius in Europe. The analysis of phenotypic features appears as a valuable complement to genetic analyses, providing a insight into evolutionary processes such as differentiation by vicariance or adaptation to different environments. Previous genetic studies based on mtDNA genes revealed a complex phylogeographic pattern for the common dormouse with the presence of two highly divergent lineages subdivided into geographic sublineages. We thus investigated whether this genetic differentiation leads to morphometric differences. We examined 150 museum specimens of dormouse from 34 localities in 12 countries. For each specimen, the size and shape of the first upper molar was quantified using an outline analysis based on Fourier methods. A geographic structure emerges from the morphometric pattern of differentiation, partially corresponding to the expectations based on the genetic results. The main differentiation, however, was unexpected and revealed a divergent Austrian cluster. This region was not documented so far for genetic analyses and morphometric data therefore challenge a more extensive sampling of this species that might reveal the existence of a further lineage. [less ▲]

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See detailLocation of the best confident scapula cluster during a forward humeral elevation
Leboeuf, Fabien; Brochard, Sylvain; Lempereur, M. et al

Poster (2011, July)

Eleven subjects were equipped with a cluster of 120 markers on the scapula. Forward humeral elevation was then performed. Robust estimators are used in order to eliminate high deformation. Results ... [more ▼]

Eleven subjects were equipped with a cluster of 120 markers on the scapula. Forward humeral elevation was then performed. Robust estimators are used in order to eliminate high deformation. Results underline the modification of the best cluster during elevation. Markers located on the lateral posterior part of the acromion were identified. They could be the best ones to measure dynamic scapula rotation during entire elevation. [less ▲]

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See detailDevelopment of non invasive genetic identification methods for the Pyrenean desman (Galemys pyrenaicus) and first study of its genetic structure in France
Gillet, François ULg; Cabria Garrido, Maria Teresa; Némoz, Mélanie et al

Poster (2011, July)

The Pyrenean desman (Galemys pyrenaicus) is probably one of the most threatened European mammal species. This small insectivorous and semi-aquatic species is endemic to the Pyrenean Mountains and of the ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is probably one of the most threatened European mammal species. This small insectivorous and semi-aquatic species is endemic to the Pyrenean Mountains and of the north of the Iberic Peninsula. Many biologic aspects of this species are currently suffering from a major lack of information, particularly those concerning its genetics. Therefore the implementation of conservative efforts for the Pyrenean desman remains extremely difficult. In order to improve the knowledge of this vulnerable species and notably, to better understand its distribution area, the first aim of our research was to develop non invasive genetic identification methods based on faeces. The second aim was to have a first look at the phylogeographic structure of the Pyrenean desman. The identification methods were developed on the basis of the sequencing of a small mitochondrial DNA (cyt b) fragment as well as a RFLP method. These approaches led to the identification of the Pyrenean desman and to the differentiation of the latter from two other species living in the same type of habitat, the white-throated dipper (Cinclus cinclus) and the water shrew (Neomys fodiens). Two haplotypes were found in the studied Pyrenean populations but no phylogeographic structure could be established. A dozen of microsatellites markers were also developed during this work and 5 of these were found to be polymorphic. The results of this preliminary work tend to show a low genetic diversity for the Pyrenean desman but this result needs to be confirmed in the future with a more extended and complete study. [less ▲]

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See detailWireworms use barley root volatile chemicals to locate their host plant in soil
Barsics, Fanny ULg; Latine, Rémi ULg; Haubruge, Eric ULg et al

Poster (2011, July)

Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for ... [more ▼]

Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for environmental issues gave birth to new needs in terms of management. In order to enhance integrated management of this pest, we work on the chemical ecology of Agriotes sordidus Illiger. We focus on its interaction with barley roots, mediated by volatile organic compounds (VOCs). Our experimentations are based on behavioural responses of wireworms in olfactometric devices and their statistical relevance. [less ▲]

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See detailTwo-level Mixtures of Markov Trees
Schnitzler, François ULg; Wehenkel, Louis ULg

Poster (2011, June 29)

We study algorithms for learning Mixtures of Markov Trees for density estimation. There are two approaches to build such mixtures, which both exploit the interesting scaling properties of Markov Trees. We ... [more ▼]

We study algorithms for learning Mixtures of Markov Trees for density estimation. There are two approaches to build such mixtures, which both exploit the interesting scaling properties of Markov Trees. We investigate whether the maximum likelihood and the variance reduction approaches can be combined together by building a two level Mixture of Markov Trees. Our experiments on synthetic data sets show that this two-level model outperforms the maximum likelihood one. [less ▲]

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See detailFASST- a FMRI Artefact rejection and Sleep Scoring Toolbox
Schrouff, Jessica ULg; Leclercq, Yves ULg; Noirhomme, Quentin ULg et al

Poster (2011, June 28)

We started writing the “fMRI artefact rejection and sleep scoring toolbox”, or “FASST”, to process our sleep EEG-fMRI data, that is, the simultaneous recording of electroencephalographic and functional ... [more ▼]

We started writing the “fMRI artefact rejection and sleep scoring toolbox”, or “FASST”, to process our sleep EEG-fMRI data, that is, the simultaneous recording of electroencephalographic and functional magnetic resonance imaging data acquired while a subject is asleep. FAST tackles three crucial issues typical of this kind of data: (1) data manipulation (viewing, comparing, chunking, etc.) of long continuous M/EEG recordings, (2) rejection of the fMRI-induced artefact in the EEG signal, and (3)manual sleep-scoring of the M/EEG recording. Currently, the toolbox can efficiently deal with these issues via a GUI, SPM8 batching system or handwritten script. The tools developed are, of course, also useful for other EEG applications, for example, involving simultaneous EEG-fMRI acquisition, continuous EEG eye-balling, and manipulation. Even though the toolbox was originally devised for EEG data, it will also gracefully handle MEG data without any problem. “FAST” is developed in Matlab as an add-on toolbox for SPM8 and, therefore, internally uses its SPM8-meeg data format. “FAST” is available for free, under the GNU-GPL. [less ▲]

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See detailHow do climate warming, plant species richness and plant functional group affect ammonia oxidizing bacteria diversity and function in experimental grasslands?
Malchair, Sandrine ULg; Carnol, Monique ULg

Poster (2011, June 27)

How do climate warming, plant species richness and plant functional group affect ammonia oxidizing bacteria diversity and function in experimental grasslands? Malchair S. and Carnol M. Laboratory of Plant ... [more ▼]

How do climate warming, plant species richness and plant functional group affect ammonia oxidizing bacteria diversity and function in experimental grasslands? Malchair S. and Carnol M. Laboratory of Plant and Microbial Ecology Department of Sciences and Environmental Management University of Liege, Belgium Background: There is increasing evidence of diversity-function relationship and impact of warming for aboveground vegetation. Belowground effects of warming and plant species richness remain however largely unknown, although bacteria regulate many soil processes and some groups, like ammonia oxidizing bacteria (AOB) were, have been suggested highly sensitive to environmental stress. Objectives: 1. Investigation of the response of AOB richness, community composition and function to warming, plant species richness and functional group 2. Exploration of the AOB richness-function link Methods: Soil samples were taken at 2 depths from grassland model ecosystems with different species richness levels (1, 3, 9) and temperature treatments (ambient, ambient+3°C). Selected species belonged to 3 plant functional groups: forbs, legumes and grasses. AOB function: potential nitrification assay (shaken soil slurry method) AOB diversity: polymerase chain reaction followed by denaturing gradient gel electrophoresis (PCR-DGGE) Results: 1. All retrieved AOB sequences were Nitrosospira-like ones 2. Warming had no effect on AOB richness and function 3. Higher plant species richness leads to increased AOB richness and modified community structure. AOB function was increased only at lower depth under warming 4. No difference in AOB richness between the plant functional groups 5. AOB community structure was different and AOB function higher under legumes. 6. The AOB richness-function link was negative under legumes. Conclusions: 1. Plant species influenced AOB richness and community composition. Plant functional group seems to be more important that species richness. 2. Legumes may impact AOB diversity and function through ammonia availability. [less ▲]

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See detailDecoding Directed Brain Activity in fMRI using Support Vector Machines and Gaussian Processes
Schrouff, Jessica ULg; Kussé, Caroline ULg; Wehenkel, Louis ULg et al

Poster (2011, June 26)

Predicting a particular cognitive state from a specific pattern of fMRI voxel values is still a methodological challenge. Decoding brain activity is usually performed in highly controlled experimental ... [more ▼]

Predicting a particular cognitive state from a specific pattern of fMRI voxel values is still a methodological challenge. Decoding brain activity is usually performed in highly controlled experimental paradigms characterized by a series of distinct states induced by a temporally constrained experimental design. In more realistic conditions, the number, sequence and duration of mental states are unpredictably generated by the individual, resulting in complex and imbalanced fMRI data sets. This study tests the classification of brain activity, acquired on 16 volunteers using fMRI, during mental imagery, a condition in which the number and duration of mental events were not externally imposed but self-generated. To deal with these issues, two classification techniques were considered (Support Vector Machines, SVM, and Gaussian Processes, GP), as well as different feature extraction methods (General Linear Model, GLM and SVM). These techniques were combined in order to identify the procedures leading to the highest accuracy measures. Our results showed that 12 data sets out of 16 could be significantly modeled by either SVM or GP. Model accuracies tended to be related to the degree of imbalance between classes and to task performance of the volunteers. We also conclude that the GP technique tends to be more robust than SVM to model unbalanced data sets. [less ▲]

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See detailModulating effect of COMT genotype on the brain regions underlying inhibition
Jaspar, Mathieu ULg; Grandjean, Julien ULg; Salmon, Eric ULg et al

Poster (2011, June 26)

Introduction Catechol-O-methytransferase (COMT) is an important enzyme which degrades catecholamines, such dopamine, notably in the prefrontal cortex (Männistö & Kaakkola, 1999). Actually, a transition of ... [more ▼]

Introduction Catechol-O-methytransferase (COMT) is an important enzyme which degrades catecholamines, such dopamine, notably in the prefrontal cortex (Männistö & Kaakkola, 1999). Actually, a transition of guanine to adenine at codon 158 of the COMT gene results in a valine to methionine substitution (Lotta & al., 1995). This phenomenon leads to different COMT genotypes, each associated with a different COMT enzymatic activity (Weinshilboum, & al., 1999). A large number of studies reported an effect of COMT on executive functioning. However, most of them used multi-determined executive tasks (e.g., Barnett & al., 2007). We are interested here to determine the effect of COMT Val158Met genotype on the activity of frontal and parietal areas (Nee & al., 2007; Laird & al., 2005) underlying the specific executive process of inhibition. Methods Procedure In an event-related fMRI experiment, a modified form of the Stroop task was administered to 44 young adults (age range: 18-30) separated into three groups according to their COMT Val158Met genotype: 15 homozygous val/val (VV), 14 homozygous met/met (MM) and 15 heterozygotes val/met (VM) carriers. The Stroop task consisted in the presentation of color words printed in various ink colors (e.g the word blue written in red). Subjects were instructed to name of ink color as fast and accurately as possible by avoiding to read the word. In this version of the Stroop task, three different contexts were created (data not showed here): (1) a congruent context (MC) with a majority of facilitator items (IC), (2) a non-congruent context (MI) with mainly interfering items (II), (3) a neutral context (MN) with mainly neutral items (IN, series of %%% written in different colors). MRI acquisition, data analysis Functional MRI time series were acquired on a 3T head-only scanner operated with the standard transmit-receive quadrature head coil. Multislice T2*-weighted functional images were acquired with a gradient-echo echo-planar imaging sequence using axial slice orientation and covering the whole brain (32 slices, FoV = 220x220 mm², voxel size 3.4x3.4x3 mm³, 30% interslice gap, matrix size 64x64x32, TR = 2130 ms, TE = 40 ms, FA = 90°). Structural images were obtained using a high resolution T1-weighted sequence (3D MDEFT [Deichmann & al., (2004)] ; TR = 7.92 ms, TE = 2.4 ms, TI = 910 ms, FA = 15°, FoV = 256 x 224 x 176 mm³, 1 mm isotropic spatial resolution). Preprocessing and statistical analyses were performed with SPM8 (p<.001 uncorrected). Results Behavioral results indicated the presence of a general interference effect (II – IN items) for reaction time (F(1,41) = 292,44 ; p < 0,001) but no significant difference in interference between the three groups (F(2,41) = 0,27; p = 0,76). FMRI results revealed that interference effect [(MI_II-MI_IN) + (MC_II-MC_IN) + (MN_II-MN_IN)] observed in our three groups is mainly associated with cerebral activity in frontal and parietal areas. Moreover, group comparisons indicates that this effect is associated with increased medial frontal and precentral gyrus activity in VV and VM groups by comparison with MM group, but also in the superior temporal gyrus and in the thalamus in the VM by comparison to MM . Conversely, no supplementary brain area was observed for the comparison of the MM to the two other groups. Conclusions The fronto-parietal brain network associated with interference resolution observed here is consistent with prior reports (Nee & al., 2007; Laird & al., 2005). Moreover, results showed activity in different brain areas according to the COMT genotype. Indeed, a similar behavioral performance is associated to the recruitment of supplementary areas in the carriers of the val allele. This observation, paralleling with the lower COMT enzymatic activity and, thus, the higher cortical dopamine level in met/met individuals, confirms our expectation of a COMT Val158Met genotype modulation of the brain regions underlying inhibition efficiency. [less ▲]

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See detailThe role of the physioterapist in physical activity promotion
Cloes, Marc ULg; MUGNIER, Benoît ULg; Mouton, Alexandre ULg

Poster (2011, June 25)

This study aimed to understand physiotherapists’ awareness about their physical activity (PA) promoting role and to analyse their actions to promote PA. 185 physiotherapists fulfilled a questionnaire ... [more ▼]

This study aimed to understand physiotherapists’ awareness about their physical activity (PA) promoting role and to analyse their actions to promote PA. 185 physiotherapists fulfilled a questionnaire related to their perceptions of (1) the PA individual motivational determinants, (2) the importance of promoting PA and (3) their actions related to people’s PA promotion. Results show that physiotherapists are aware of their crucial role in PA promotion. Nevertheless, their actions should be more devoted to the prevention and detection of sedentary behaviours. Moreover, connections with other actors of PA promotion should be emphasized in order to respect the principles of an ecological approach. [less ▲]

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See detailPhysiotherapists' representations about physical activity
Mouton, Alexandre ULg; MUGNIER, Benoît ULg; Cloes, Marc ULg

Poster (2011, June 24)

This study aimed to analyze the knowledge and representations of physiotherapists about the physical activity (PA) key concepts. 185 physiotherapists fulfilled a questionnaire related to their knowledge ... [more ▼]

This study aimed to analyze the knowledge and representations of physiotherapists about the physical activity (PA) key concepts. 185 physiotherapists fulfilled a questionnaire related to their knowledge about PA (1) definition, (2) benefits for health, (3) criterion for an effective practice and (4) recommendations. The results shows that the physiotherapists’ knowledge about the key concepts of PA and its implications for PA promotion are surprisingly low. Even if they are aware about the population actual lack of PA practice, their recommendations provided to the public would be inaccurate. Future directions for a more effective action of the physiotherapists as PA promoters are discussed. [less ▲]

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See detailCharacteristics and evolution of hantavirus infection in Liège Area (Belgium)
Krzesinski, Jean-Marie ULg; parotte, Marie-Christine; Vanden velde, Christian

Poster (2011, June 24)

Hantavirus is a zoonosis transmitted by rodents responsible in Europe for Hemorrhagic fever with acute renal syndrome (called nephropathia epidemica or NE). Hantavirus epidemics were increased in the ... [more ▼]

Hantavirus is a zoonosis transmitted by rodents responsible in Europe for Hemorrhagic fever with acute renal syndrome (called nephropathia epidemica or NE). Hantavirus epidemics were increased in the recent years in the South part of Belgium. After acute illness, the question could be about its evolution. [less ▲]

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See detailError distribution estimation in right censored and selection biased location-scale models
Laurent, Géraldine ULg; Heuchenne, Cédric ULg

Poster (2011, June 23)

Suppose the random vector (X;Y) satis es the regression model Y = m(X)+sigma(X)*epsilon where m(X) = E[Y|X] and sigma²(X) = Var[Y|X] are unknown smooth functions and the error epsilon, with unknown ... [more ▼]

Suppose the random vector (X;Y) satis es the regression model Y = m(X)+sigma(X)*epsilon where m(X) = E[Y|X] and sigma²(X) = Var[Y|X] are unknown smooth functions and the error epsilon, with unknown distribution, is independent of the covariate X. The pair (X;Y) is subject to generalized selection biased and the response to right censoring. We construct a new estimator for the cumulative distribution function of the error epsilon, where the estimators of m(.) and sigma²(.) are obtained by extending the conditional estimation methods introduced in de Uña-Alvarez and Iglesias-Perez (2010). The asymptotic properties of the proposed estimator are established. A bootstrap technique is proposed to select the smoothing parameter involved in the procedure. This method is studied via extended simulations and applied to real unemployment data. Reference de UNA-ALVAREZ, J., IGLESIAS-PEREZ, M.C. (2010): Nonparametric estimation of a conditional distribution from length-biased data. Annals of the Institute of Statistical Mathematics, Vol. 62, 323-341. [less ▲]

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See detailImpurity fingerprints for the identification of counterfeit medicines - a feasibility study
Sacré, Pierre-Yves ULg; deconinck, Eric; Daszykowski, Michal et al

Poster (2011, June 22)

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See detailThe attachment of Bacillus amyloliquefaciens FZB42 to tomato roots is impaired in a mutant devoid of GalM and PBP4a.
Van der Heiden, Edwige; Jourdan, Emmanuel ULg; Delmarcelle, Michaël ULg et al

Poster (2011, June 20)

In vivo experiments on tomato (Lycopersicon esculentum) roots have been performed: drench of 10-day hydroponic cultures with bacterial suspensions. A second identical treatment was applied one week later ... [more ▼]

In vivo experiments on tomato (Lycopersicon esculentum) roots have been performed: drench of 10-day hydroponic cultures with bacterial suspensions. A second identical treatment was applied one week later and after 30 days of culture, the plants were collected and the bacteria detached from a precise weight of roots were diluted, plated and counted. The mutant devoid of both GalM and PBP4a (encoded by the galM and dacC operon) is affected in its capacity to colonize the tomato roots. [less ▲]

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See detailHigh Throughput determination of Levonorgestrel in human plasma using a Sensitive LC-MS/MS method
Hubert, Cédric ULg; Streel, Bruno; Sibenaler, Renilde et al

Poster (2011, June 19)

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See detailReversibly core-cross-linked micelles sensitive to reductive environment for the design of drug delivery systems
Cajot, Sébastien ULg; Danhier, F.; Collodoro, M. et al

Poster (2011, June 16)

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See detailThe recent introduction of Lamprichthys tanganicanus in Lake Kivu (Eastern Africa): a threat for the pelagic fishery?
Masilya, Pascal M; Isumbisho, Mwapu; Kaningini, M et al

Poster (2011, June 13)

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See detailA RAPID UHPLC-DAD-ESI-MSn METHOD FOR ANTHOCYANINS QUANTIFICATION FROM Euterpe oleracea FRUITS HARVESTED AT DIFFERENT TIMES
Dias, A.; Chataigné, G.; Rozet, Eric ULg et al

Poster (2011, June 09)

Euterpe oleracea is a palm tree widely distributed in northern South America. Its greatest occurrence and economic importance happens in the floodplains of the Amazonian delta. The fruits called açai are ... [more ▼]

Euterpe oleracea is a palm tree widely distributed in northern South America. Its greatest occurrence and economic importance happens in the floodplains of the Amazonian delta. The fruits called açai are an interesting source of different anthocyanins. Lately they have gained popularity in North America and in the European countries in the food industry and in the health sector due to their extremely high antioxidant capacity and potential anti-inflammatory activities [1]. Some studies have characterized chemically açaí pulps and have reported anthocyanin profiles which differ both qualitatively and quantitatively. Among other reasons, these differences may be associated with the stages of ripening of the fruits, since açai fruits are generally harvested in different maturation stages. The evaluation of the anthocyanin profile of açai fruits during different maturation stages is thus important for the post-harvest industry. In addition a rapid separation by UHPLC and an unambiguous identification by MSn are very useful for an effective quality control of the fruits. Thus, the aim of this study was to characterize the anthocyanin profiles of açai fruits at different stages of maturity. The fruits were harvested during the peak harvesting season, between July and October 2009, in the floodplains of the eastern Amazonian region (State of Pará, Brazil). A protocol of solid-liquid extraction of phenolic compounds was developed. Characterisation of the anthocyanins present in the fruits of Euterpe oleracea was conducted by UHPLC–DAD–ESI–MSn analysis, in positive ionization mode. All identified compounds was separated in 10 minutes of a total run time of 21 min instead of 55 min in the previously developed HPLC method. Six anthocyanins were identified in the extracts namely: cyaniding-3-glucoside, cyaniding-3-rutinoside, pelargonidin-3-glucoside, peonidin-3-glucoside, peonidin-3-rutinoside and cyanidin. The first two compounds were the major constituents in all maturity stages, with similar proportions, except for the first maturity stage for which the anthocyanins were under the limit of quantification. However, in the last maturity stage, cyanidin-3-glucoside became less abundant than cyanidin-3-rutinoside. On the other hand, cyanidin decreased with maturation. For the other compounds, proportions were similar along maturation. Hence, this work was important as it provides valid information on variation of anthocyanin profiles of açai fruits during maturation. This may contribute to the selection of an optimal maturity stage for harvesting as well as it will allow a rapid quality control of the fruits. [1]: Heinrich, M., et al., Açai (Euterpe oleracea Mart.)- A phytochemical and pharmacological assessment of the species’ health claims. Phytochem. Lett. (2010), doi: 10.1016/j.phytol.2010.11.005 [less ▲]

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See detailAnalysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULg; De Lemos Esteves, Frédéric ULg; Natalis, Lucie et al

Poster (2011, June 09)

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ... [more ▼]

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs. [less ▲]

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See detailAn integrative model based approach to optimize calcium phosphate scaffold-stem cell combinations
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2011, June 07)

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See detailNovel drug delivery sytem of siRNA based on chitosan, pegylated chitosan and polyethyleneimine
Ragelle, Héloïse; Vandermeulen, Gaëlle; Riva, Raphaël ULg et al

Poster (2011, June 06)

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See detailDisruption of PDZ protein-protein interactions inhibits Tax transformation and HTLV-1 infection capacities.
Twizere, Jean-Claude ULg; DEWULF, Jean-François; Blibek, Karim ULg et al

Poster (2011, June 06)

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its ... [more ▼]

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its C-terminus that is essential for its transforming activity in a Rat-1 model and for IL-2. Tax has been shown to interact with several PDZ domain containing proteins including PSD-95, Beta1-syntrophin, the precursor of interleukin-16, the mammalian homolog of the Drosophila discs large tumor suppressor protein Dlg, PDLIM2, Lin7, hTid1, Tip1, hScrib and MAGI3. In the 15th International Conference on Human Retrovirology: HTLV and Related Retroviruses, we will present a specificity map for the Tax/PDZ domain interactions generated using the human ORFeome 5.1. and we will focus on some of the new Tax/PDZ interactions. [less ▲]

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See detailSpecific contribution of short-term memory for serial order information to early reading acquisition: A longitudinal study
Martinez Perez, Trecy ULg; Majerus, Steve ULg; Poncelet, Martine ULg

Poster (2011, June 02)

Early reading acquisition skills have been linked to verbal short-term memory (STM) capacity. However, the nature of this relationship remains controversial. Here we distinguished between STM for item and ... [more ▼]

Early reading acquisition skills have been linked to verbal short-term memory (STM) capacity. However, the nature of this relationship remains controversial. Here we distinguished between STM for item and order information based on recent studies showing that STM for order is an important and independent predictor of oral language development. Tasks maximizing STM for serial-order or item information and reading tests were administered to 42 children from kindergarten through 1st grade. Results showed that order STM capacity measured at kindergarten predicted phonological recoding abilities at 1st grade. Implications of poor serial-order STM for reading acquisition in dyslexia are discussed. [less ▲]

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See detailPotentialities of using ASTER & SRTM DEM for road planning in Central African sustainable forest logging context. A case study in East Gabon
Philippart, Julien ULg; Handerek, Daphné ULg; Lejeune, Philippe ULg et al

Poster (2011, June 02)

Slope is the main constraint for sustainable forest road planning in Central Africa. Remote sensing now provides free and downloadable Data Elevation Model (DEM) covering most of appeared lands. In this ... [more ▼]

Slope is the main constraint for sustainable forest road planning in Central Africa. Remote sensing now provides free and downloadable Data Elevation Model (DEM) covering most of appeared lands. In this study, we evaluate potentialities and limitations of Shuttle Radar Topography Mission (SRTM) DEMs, derived from radar interferometry and Advanced Spaceborn Thermal Emission and Reflection Radiometer (ASTER) DEMs, themselves derived from digital photogrammetry for slope evaluation. Assessment is presented for hilly (Milole) and flat (Okondja) areas. Three elevation maps were derived from initial ASTER 30-m and SRTM 90-m DEMs : a SRTM 30-m resampled from SRTM 90-m and two ASTER 30-m where absurd values (artifacts) were corrected with SRTM 90-m and resampled SRTM 30-m respectively. We qualitatively and quantitatively assess the accuracy of all elevation maps compared to 992 (698) slope measures on field along transects of 10.5 (7.5) km in Milolé (Okondja). We estimated root mean square error (RMSE) for slope estimation at 7.8 (10.7), 8.1 (10.1), 11.7 (11.2), 10.1 (11.2) and, 9.3° (11.0°) for SRTM 90-m, SRTM 30-m, ASTER 30-m, ASTER 30-m CORR 90 and ASTER 30-m CORR 30 respectively in Milolé (Okondja). We also use a classification error matrix to assess Global Accuracy (GA) and User’s Accuracy (UA) of elevation maps by classifying ground slopes in two categories: lower or equal and higher than maximum slope limitation of 12% (30%) for primary (secondary) roads. All DEMs show a greater GA in hilly area (Milolé) than in flat area (Okondja) and SRTM derived DEMs show a higher UA for secondary roads constraint. UA for the lower or equal category varies between 55.5 and 75.2% (63.9 and 91.7%) for primary (secondary) roads. The use of corrected aster DEMs increases initial ASTER UA from 0.1 to 18.8% depending on category and slope limitation. Despite a relatively high RMSE for slope grade, all of the DEMs tested were found to be consistent for consideration of maximum slope constraint aiming at sustainable road planning for forest logging in Central Africa. [less ▲]

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See detailVoice Impact of a Prolonged Reading Task at two Intensity Levels
Remacle, Angélique ULg; FINCK, Camille ULg; Roche, Anne et al

Poster (2011, June 02)

The purpose of this study is to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading. Fifty normophonic women undergo two sessions of voice loading in ... [more ▼]

The purpose of this study is to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading. Fifty normophonic women undergo two sessions of voice loading in which the required intensity level of voice varied: 60-65 dB(A) for the first session, and 70-75 dB(A) for the second session. Serial voice quality objective data is obtained every 30 minutes during the oral reading tasks, by use of Multi-Dimensional Voice program (Average fundamental frequency (AvF0), Jitter percent (Jitt), Shimmer percent (Shim), Noise-to-Harmonic Ratio (NHR)). Serial measurements of voice range are also carried out every 30 minutes of the oral reading tasks, by use of the voice range profile. Results indicate that AvF0, lowest frequency of voice range and softest output of phonation rise during prolonged oral reading. On the contrary, Jitt tends to decrease and Shim decreases significantly with the passage of time during the voice loading. NRH does not vary statistically. AvF0 and highest outpout of phonation are the only parameters influenced by the required intensity of oral reading task: they are significantly higher in the 70-75 dB session compared to the 60-65 dB session. [less ▲]

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See detailInfluence of thermoelectric coupling on pacemaker activity generated by mechano-electric feedback in a one-dimensional ring-shaped model of cardiac fiber
Collet, Arnaud ULg; Desaive, Thomas ULg; Pierard, Luc ULg et al

Poster (2011, June 01)

The mechano-electric feedback (MEF) in the heart consists in the influence of the tissue deformations on the cardiac electrical activity. Under certain conditions, tissue deformations can generate ... [more ▼]

The mechano-electric feedback (MEF) in the heart consists in the influence of the tissue deformations on the cardiac electrical activity. Under certain conditions, tissue deformations can generate electrical perturbations via stretch-activated channels, such that the membrane potential can exceed the threshold value needed in order to trigger cardiac action potentials (APs). In the present study, we have developed a one-dimensional ring-shaped model of cardiac fiber taking into account three different couplings: the excitation-contraction coupling (ECC), the MEF and the thermoelectric coupling (TEC). The main goal of this work is to examine the effects of the TEC on the different properties of the pacemaker activity generated by the MEF. [less ▲]

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See detailTowards the understanding of the structure and assembly of the E. coli septum
Derouaux, Adeline ULg; Vollmer, Waldemar

Poster (2011, June)

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See detailStructure-Surface Activity Relationships Of Uronic Acid-Derivative Surfactants From Renewable Resources Applications
Razafindralambo, Hary ULg; Richel, Aurore ULg; Richard, Gaetan ULg et al

Poster (2011, June)

Introduction and Background: Uronic acid-derivative surfactants are amphiphilic compounds with growing interests owing to their potential applications in various areas [1]. This class of carbohydrate ... [more ▼]

Introduction and Background: Uronic acid-derivative surfactants are amphiphilic compounds with growing interests owing to their potential applications in various areas [1]. This class of carbohydrate-based surfactants can be easily prepared from renewable raw materials in a wide range of structure by modular synthesis thanks to the presence of one carboxylic and numerous hydroxyl groups. The polar head group configurations according to the stereochemistry of OH groups, and geometry (cyclic or not), the hydrophobic tail (number and length of alkyl chain), and the type of linker (ester, acetal and amide, etc) are among the main variables in their structural entities. Therefore, the investigation of their structure-surface activity relationships appears valuable for increasing backgrounds, and achieving a rational design for selecting the best structures to be used in different industrial fields [2]. Methodology: A few analogous of glucuronic acid-derivative surfactants have been synthesized by chemical or enzymatic routes. After purification, their structure has been confirmed by various spectroscopic techniques (RMN, MS, IR). Surface tensions of true aqueous solution have been then measured in dynamic and static modes using a series of complementary techniques. Critical micelle concentrations, minimum molecular areas, and maximum surface excesses have also been determined. Results and Discussion: Glucuronic acid derivative surfactants under investigation vary in the polar head group configuration including cyclic or non-cyclic structure and α or β anomeric form, in the hydrophobic tail regarding to the chain length (C8 to C14), the presence of a double bond, as well as an OH group at the terminal carbon, and in the type of linker, ester in C6 or acetal in C1. Results showed that all of these structural attributes affect both dynamic and equilibrium surface properties of glucuronic acid -based surfactants. Conclusion: A set of synthetic glucuronic acid-based surfactants varying in the polar head group, hydrophobic tail, and linker allow us to generate various surface-active properties at the air-water interface, and to increase the knowledge on relationships between their structure and surface-active properties. [1] Laurent, P.; Razafindralambo, H.; Wathelet, B.; Blecker, C.; Wathelet, J.-P.; Paquot, M., Synthesis and Surface-Active Properties of Uronic Amide Derivatives, Surfactants from Renewable Organic Raw Materials. Journal of Surfactants and Detergents 2010, in press. [2] Razafindralambo, H.; Blecker, C.; Mezdour, S.; Deroanne, C.; Crowet, J.; Brasseur, R.; Lins, L.; Paquot, M., Impacts of the Carbonyl Group Location of Ester Bond on Interfacial Properties of Sugar-Based Surfactants: Experimental and Computational Evidences. The Journal of Physical Chemistry B 2009, 113 (26), 8872-8877. Acknowledgment: This work was supported by Belgian Walloon Region within DGTRE research project of excellence (TECHNOSE). [less ▲]

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See detailKynurenic acid is able to suppress cortical spreading depression and the effect size is sex hormone dependent
Chauvel, Virginie ULg; Vamos, Eniko; Pardutz, Arpat et al

Poster (2011, June)

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See detailModeling post-combustion CO2 capture with amine solvents
Léonard, Grégoire ULg; Heyen, Georges ULg

Poster (2011, June)

Carbon capture and storage is a technology that can contribute to face the challenge of rising energy demand combined with a growing environmental awareness. In the present work, the CO2 capture process ... [more ▼]

Carbon capture and storage is a technology that can contribute to face the challenge of rising energy demand combined with a growing environmental awareness. In the present work, the CO2 capture process with monoethanolamine (MEA) is modeled using the simulation tool Aspen Plus. Two different modeling approaches are studied and compared: the equilibrium and the rate-based approaches. An optimization of key process parameters is performed and process modifications are studied with the objective of improving the global process energy efficiency. [less ▲]

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See detailEffect of blue monochromatic light on non-visual functions in aging
Daneault, V; Vandewalle, Gilles ULg; Teikari, P et al

Poster (2011, June)

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See detailCOMPARATIVE STUDY OF SPR BIOSENSORS BASED GOLD AND SILVER COLLOIDAL NANOPARTICLES
Lismont, Marjorie ULg; Dreesen, Laurent ULg

Poster (2011, June)

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which ... [more ▼]

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which corresponds to the collective oscillations of the conduction electrons on the particles [1]. Among metals, silver and gold NPs are of particular interest not only because they are air-stable but also because their SPR absorption bands are in the visible and near ultra-violet spectral regions, that appear as the most appropriate for technological applications [2]. The first advantage of such optical SPR biosensors is their ability to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Because of the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. Biotin is a water-soluble B complex vitamin necessary for the production of fatty acids and the metabolism of fats and amino acids. The avidin is a tetrameric protein which can react with biotin to form the strongly bonded biotin-avidin complex.The prototypical biotin-avidin interaction forms the basis of a simple sol-based diagnostic technique for biological analytes. We focused on this well-known couple of bio-molecules to compare optical properties of silver and gold colloidal NPs. Gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs were studied as a function of added avidin. After avidin addition, an increased red-shift of the SPR wavelength and a broadening of the absorption band with time are observed. These changes in the optical properties of colloidal NPs are due to the biomolecular recognition process between biotin and avidin which leads to aggregation of these NPs arising from cross-linking by the tetrameric protein. Moreover, the recognition process induces a variation of the local refractive index around these NPs and thus induces a red-shift of SPR also. The maximum SPR red-shift was reached after 45 minutes and was equal to 25 nm and 15 nm for silver NPs and gold NPs respectively. We concluded that the dielectric sensitivity of gold NPs is smaller than the silver NPs one for a same geometry and for an equivalent concentration of avidin. Therefore, the silver sol is more adapted to detection of avidin than the gold sol. The detection limit, described as the lowest concentration for clear identification of wavelength shift [4] due to biomolecular recognition is determined to be 4 nM for both silver and gold NPs. In this case, the corresponding wavelength shift is about 3 nm. The specificity of the interaction between biocytin and avidin was checked by replacing avidin by BSA. When BSA was added, we observed a SPR shift which was smaller than the detection limit of 3 nm. Future works will be devoted to transpose this kind of biomolecular recognition experiments on gold nanorods in order to improve the dynamic phototherapy efficiency of cancers. [less ▲]

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See detailAn easy-to-use pipeline for creating connectomes
Ziegler, Erik ULg; Foret, Ariane; Matarazzo, Luca et al

Poster (2011, June)

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See detailMolecular histology for epithelial ovarian cancers biomarker hunting: new issues for biology and pharmacology.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, June)

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See detailThe perceptual and verbal analogical reasoning in children with specific language impairment (SLI)
Leroy, Sandrine ULg; Guénébaud, Mélanie; Parisse, Christophe et al

Poster (2011, June)

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See detailDiallyl disulfides: comparison between classical and microwave assisted synthesis
DETHIER, Bérénice ULg; Richel, Aurore ULg; Laurent, Pascal ULg et al

Poster (2011, June)

Garlic contains organosulfur compounds, such as diallyl disulfides (DADS), diallyl monosulfides (DAMS) and diallyl trisulfides (DATS), which have potential health properties. The first objective of this ... [more ▼]

Garlic contains organosulfur compounds, such as diallyl disulfides (DADS), diallyl monosulfides (DAMS) and diallyl trisulfides (DATS), which have potential health properties. The first objective of this work is to conduct the synthesis of these molecules by heating in a classical oil bath or by microwave irradiation with a phase transfer catalyst. We concluded that the synthesis should be cnducted at 40°C in an oil bath, with a phase transfer catalyst. [less ▲]

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See detailDefining Antonymy by studying Co-occurrences in Context
Steffens, Marie ULg

Poster (2011, June)

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See detailA cytokinin route to flowering in Arabidopsis
Bouché, Frédéric ULg; André, Julie ULg; D'Aloia, Maria ULg et al

Poster (2011, June)

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes ... [more ▼]

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes flowering in non-inductive short days. The response to BAP treatment does no require FLOWERING LOCUS T (FT), but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) (D'Aloia et al., 2011). We present here complementary data obtained with transgenic plants overexpressing a catalytic CK OXIDASE/DEHYDROGENASE (CKX) in the roots. The high efficiency of BAP in promoting flowering in our experimental system contrasts with the variability that emerges from studies gathered in literature. Many factors, either experimental or inherent to plant material, might explain these discrepancies and we are interested in identifying endogenous regulators that might provide a mechanistic explanation. We are therefore investigating whether the endogenous pathways underlying plant developmental phase changes might regulate the relative contribution of CKs to flowering. [less ▲]

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See detailOn generalized Hölder spaces
Kreit, Damien ULg; Nicolay, Samuel ULg

Poster (2011, June)

The Hölder spaces provide a natural way for measuring the smoothness of a function. These spaces appear in different areas such as approximation theory and multifractal analysis and lead to natural ... [more ▼]

The Hölder spaces provide a natural way for measuring the smoothness of a function. These spaces appear in different areas such as approximation theory and multifractal analysis and lead to natural definitions of the notion of fractal function; for example a function belonging to $C^\alpha$ with $\alpha\in (0,1)$ typically has a fractal graph. The purpose of this poster is to present a generalization of such spaces as well as some recent results about their characterizations. [less ▲]

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See detailTranscriptomic analysis of Arabidopsis roots during floral induction by photoperiod
D'Aloia, Maria ULg; Lamoureux, Thibaut ULg; Tocquin, Pierre ULg et al

Poster (2011, June)

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an ... [more ▼]

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an hydroponic device that allows synchronous growth and flowering of Arabidopsis and performed global transcript profiling of roots. Samples were harvested during the extension period of a single long day (LD), and in non inductive short day. Microarray data were validated by real-time RT-PCR, and the expression patterns of selected probes were further analyzed in shoots and roots. Some of the genes that were differentially expressed in the roots during the inductive LD did not show the same variations in the shoot, indicating that root transcriptome undergoes specific changes at floral transition. These genes include, for example, GIGANTEA. T-DNA mutants from selected candidate genes are being studied. Both the expression analysis and the reverse genetic approach provide new insights into the contribution of the roots to the flowering process. [less ▲]

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See detailAnt-aphid mutualism - How do ants locate aphid colonies?
Fischer, Christophe ULg; Sablon, Ludovic ULg; Duriaux, Adrien ULg et al

Poster (2011, June)

That some ant species show mutualistic relationships with some aphid species is well known since several decades. Ants protect aphids against natural enemies and as a reward collect the sugar-rich aphid ... [more ▼]

That some ant species show mutualistic relationships with some aphid species is well known since several decades. Ants protect aphids against natural enemies and as a reward collect the sugar-rich aphid excretion called honeydew. However, very little information is available on possible semiochemicals involved in these interactions. This study aims to identify semiochemicals involved in the establishment of this relation, using both chemical and behavioral approaches. Preliminary choice tests confirmed that ant scouts are able to detect aphid colonies at a distance using volatile organic compounds (VOC). Those VOC influencing ant’s orientation may originate from different sources: the attacked plant, the honeydew produced by the aphids and the aphids themselves. VOC emission profiles of each of those potential sources have been established using gas chromatography. Y-olfactometer assay revealed that honeydew volatile compounds are the key element in this distant attraction. Moreover, based on these VOCs, ant scouts are able to discriminate between the honeydew produced by a myrmecophilous and a non myrmecophilous aphid species. Thus, more than just a sugar source, honeydew is an attractant for ant scouts towards myrmecophilous aphids. [less ▲]

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See detailFunctional and physicochemical properties of starches isolated from pearl millet landraces grown in hyperarid regions
Boudries, Nadia; Sindic, Marianne ULg; Nadjemi, Boubekeur et al

Poster (2011, May 31)

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See detailKraft RB : recurrent neural network prediction of steam production
Sainlez, Matthieu ULg; Heyen, Georges ULg

Poster (2011, May 30)

In this study, neural networks approaches are compared for predicting the high pressure (HP) steam flow rate from a Kraft recovery boiler. We apply two types of neural networks: a static multilayer ... [more ▼]

In this study, neural networks approaches are compared for predicting the high pressure (HP) steam flow rate from a Kraft recovery boiler. We apply two types of neural networks: a static multilayer perceptron and a dynamic Elman’s recurrent neural network. Starting from a one-day database of raw process data related to the boiler, the goal is to model and predict the next 12-hours of HP steam flow production from the boiler to the steam turbine. The results illustrate the potential of the dynamic approach in this task. [less ▲]

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See detailRelations between a computerized shopping task and cognitive and clinical variables in patients diagnosed with bipolar disorder and alcohol dependency compared with healthy controls.
Laloyaux, Julien ULg; Michel, Céline; Pellegrini, Nadia et al

Poster (2011, May 27)

Persons diagnosed with bipolar disorder and alcohol dependency are frequently subject to poor everyday life functioning. However, previous studies have primarily used questionnaires or observational ... [more ▼]

Persons diagnosed with bipolar disorder and alcohol dependency are frequently subject to poor everyday life functioning. However, previous studies have primarily used questionnaires or observational methods to assess everyday life functioning, both of which contain a number of limits. In order to address some of these limits, we developed a computerised real-life activity task, in particular, a shopping task where participants are required to shop for a list of 8 grocery store items. Twenty individuals diagnosed with alcoholic dependence and 21 patients diagnosed with bipolar disorder were compared with 20 and 21 matched healthy controls, respectively. All participants completed the shopping task, and both clinical groups were evaluated with an extensive battery of neuropsychological tests and a measure of global functioning. Results showed that, for both clinical groups, performance on the computerised shopping task significantly differentiated patients and healthy controls for a number of variables, especially total time and mean time to consult the shopping list. Performances on shopping task variables, in both clinical groups, were also significantly correlated with neuropsychological tests measuring verbal episodic memory, processing speed and selective attention. Finally, performances on the computerised shopping task were significantly correlated with various clinical variables and with global functioning in both patient groups. These findings suggest that the computerised task used in the present study provides a valid indication of the level of everyday life functioning for these clinical populations, and therefore may be viewed as a valuable instrument in both an evaluation and remediation context. [less ▲]

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See detailEfficacy of errorless learning in the acquisition of a new procedural skill in Alzheimer's disease
Schmitz, Xavier ULg; Lejeune, Caroline ULg; Vervecken, Nancy et al

Poster (2011, May 27)

In Alzheimer’s disease (AD), implicit or procedural rehabilitation techniques would be more effective to train new skills than explicit or declarative learning methods (van Halteren-van Tilborg, 2007 ... [more ▼]

In Alzheimer’s disease (AD), implicit or procedural rehabilitation techniques would be more effective to train new skills than explicit or declarative learning methods (van Halteren-van Tilborg, 2007). Following Baddeley and Wilson (1994)’s assumption, Maxwell et al. (2001) showed that reducing errors during motor learning minimizes the building of declarative knowledge and would allow implicit knowledge accumulation. However, most studies on errorless learning focused on learning of face-name associations (Clare et al., , 2001), and very few studies have investigated errorless learning in procedural learning situations, even though some data suggest that errorless learning would be efficient for learning instrumental activities of daily living (e.g., Thivierge et al., 2008). The aim of this study was to examine the efficacy of an error-reducing versus an errorfull method in motor skill learning. We examined the acquisition of a new motor skill in 24 patients with AD and 24 healthy older adults matched for age, sex, and education. In this task, subjects had to follow with a reversed mouse the contour of a form (a star) displayed on a computer screen. Half the subjects learned in an error-reducing condition, and the others in an errorfull condition. After the learning phase, all the subjects had to complete a novel form. Results show an advantage for the error-reducing condition in the AD group, whereas the performance of the healthy participants did not differ between the two conditions, confirming the efficiency of errorless learning principles in AD for procedural learning situations. [less ▲]

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See detailThe "other-age" effect in age estimation of faces
Moyse, Evelyne ULg; Manard, Marine ULg; Brédart, Serge ULg

Poster (2011, May 27)

In this study the occurrence of an “other-age” effect on age estimation was assessed. This effect consists in a more accurate estimation of own-age faces than other-age faces. Here, the age of ... [more ▼]

In this study the occurrence of an “other-age” effect on age estimation was assessed. This effect consists in a more accurate estimation of own-age faces than other-age faces. Here, the age of participants (10 to 14 year old children and 20 to 30 year old adults) and the age of stimuli (faces of 10 to 14 year old children and faces of 20 to 30 year old adults) were crossed. Although we did not observe a crossed interaction where each age group would have been more accurate for in-group estimation than for out-group estimation, present results revealed the occurrence of an own-age bias in age estimation. Although both groups did not differ when estimating child faces, adults were more accurate than children when estimating the age of adult faces. Therefore, the present results showed an asymmetrical "other-age" effect. Several hypotheses explaining the present pattern of results are discussed. [less ▲]

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See detailEmergency physicians’ communication: What about a standardized handover?
Gillet, Aline ULg; D'ORIO, Vincenzo ULg; Nyssen, Anne-Sophie ULg

Poster (2011, May 27)

In health care organizations, the need for 24-hour care increases the number of patient’s transfer. Handover and shift changes are now recognized as particularly critical moments for the reliability of ... [more ▼]

In health care organizations, the need for 24-hour care increases the number of patient’s transfer. Handover and shift changes are now recognized as particularly critical moments for the reliability of care. However, few studies focused specifically on how physicians share relevant information during these moments. Moreover, emergency departments are known to be extremely vulnerable to error, because of high time pressure, frequent interruptions, high variability and number of patients, etc. Our study aimed to estimate how emergency physicians share information about a patient during handover, and to evaluate the impact of a procedure of medical handover standardization. To do this, we conducted a pre-post test on completeness of transmissions. We first performed observations of 50 physicians’ shift changes in an emergency department. All of these were video-recorded and then analyzed. We classified communications into seven categories (identifying information, current pathology, patient’s current status, significant patient’s tests results, priority medical interventions, diagnose and recommendations, and dispositions). After these observations, we elaborated and implemented a standardization procedure, according to literature, analyses of our observations and physicians’ recommendations. We finally assessed this procedure by observing and analyzing 40 handovers, using the same method as previously described. We also evaluated the physician’s opinion about the quality of the transmission with a 7-point Likert scale. Our results showed three significant differences before and after the standardization of communications. Physicians share more information about patient’s tests results, priority interventions and dispositions. Moreover, we found a significant difference of the perceived quality of the standardized handover. [less ▲]

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See detailDevelopmental differences in the procedural learning of a perceptual-motor skill
Lejeune, Caroline ULg; Catale, Corinne ULg; Merbah, Sarah ULg et al

Poster (2011, May 27)

It is generally admitted that procedural learning is efficient early in childhood. However, few studies have brought empirical data confirming this assumption, and many questions remain regarding the ... [more ▼]

It is generally admitted that procedural learning is efficient early in childhood. However, few studies have brought empirical data confirming this assumption, and many questions remain regarding the cognitive mechanisms that sustain procedural learning in children. The aim of our study was to investigate whether perceptual-motor procedural learning was present to the same extent in 7-, 10-year-old children and in adults. We also examined the role of executive functions, working memory, general intelligence, and motor ability during the learning process. A total of 76 subjects divided into 3 age-groups were tested. The task included 4 blocks of 3 trials during which each subject had to trace the contour of a triangle with an inverted computer mouse. Analyses show an important difference between groups in the initial phase of the learning process. They also reveal that executive functions intervene during the first learning phase, which might explain the observed age effect. In addition, results show significant but different learning effects for the procedural task: while the improvement was equivalent between 10-year-olds and adults, 7-year-old children showed a greater learning slope than the other groups; despite their slowness during the first blocks, younger children showed an equivalent performance at the end of the learning phase. These results suggest that, if executive processes are important during the first learning steps, they are not a “necessary condition” for motor skill learning to occur. The role of compensatory strategies sustaining learning in younger children is discussed. [less ▲]

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