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See detailAn integrative model based approach to optimize calcium phosphate scaffold-stem cell combinations
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2011, June 07)

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See detailNovel drug delivery sytem of siRNA based on chitosan, pegylated chitosan and polyethyleneimine
Ragelle, Héloïse; Vandermeulen, Gaëlle; Riva, Raphaël ULg et al

Poster (2011, June 06)

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See detailDisruption of PDZ protein-protein interactions inhibits Tax transformation and HTLV-1 infection capacities.
Twizere, Jean-Claude ULg; DEWULF, Jean-François; Blibek, Karim ULg et al

Poster (2011, June 06)

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its ... [more ▼]

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its C-terminus that is essential for its transforming activity in a Rat-1 model and for IL-2. Tax has been shown to interact with several PDZ domain containing proteins including PSD-95, Beta1-syntrophin, the precursor of interleukin-16, the mammalian homolog of the Drosophila discs large tumor suppressor protein Dlg, PDLIM2, Lin7, hTid1, Tip1, hScrib and MAGI3. In the 15th International Conference on Human Retrovirology: HTLV and Related Retroviruses, we will present a specificity map for the Tax/PDZ domain interactions generated using the human ORFeome 5.1. and we will focus on some of the new Tax/PDZ interactions. [less ▲]

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See detailPotentialities of using ASTER & SRTM DEM for road planning in Central African sustainable forest logging context. A case study in East Gabon
Philippart, Julien ULg; Handerek, Daphné ULg; Lejeune, Philippe ULg et al

Poster (2011, June 02)

Slope is the main constraint for sustainable forest road planning in Central Africa. Remote sensing now provides free and downloadable Data Elevation Model (DEM) covering most of appeared lands. In this ... [more ▼]

Slope is the main constraint for sustainable forest road planning in Central Africa. Remote sensing now provides free and downloadable Data Elevation Model (DEM) covering most of appeared lands. In this study, we evaluate potentialities and limitations of Shuttle Radar Topography Mission (SRTM) DEMs, derived from radar interferometry and Advanced Spaceborn Thermal Emission and Reflection Radiometer (ASTER) DEMs, themselves derived from digital photogrammetry for slope evaluation. Assessment is presented for hilly (Milole) and flat (Okondja) areas. Three elevation maps were derived from initial ASTER 30-m and SRTM 90-m DEMs : a SRTM 30-m resampled from SRTM 90-m and two ASTER 30-m where absurd values (artifacts) were corrected with SRTM 90-m and resampled SRTM 30-m respectively. We qualitatively and quantitatively assess the accuracy of all elevation maps compared to 992 (698) slope measures on field along transects of 10.5 (7.5) km in Milolé (Okondja). We estimated root mean square error (RMSE) for slope estimation at 7.8 (10.7), 8.1 (10.1), 11.7 (11.2), 10.1 (11.2) and, 9.3° (11.0°) for SRTM 90-m, SRTM 30-m, ASTER 30-m, ASTER 30-m CORR 90 and ASTER 30-m CORR 30 respectively in Milolé (Okondja). We also use a classification error matrix to assess Global Accuracy (GA) and User’s Accuracy (UA) of elevation maps by classifying ground slopes in two categories: lower or equal and higher than maximum slope limitation of 12% (30%) for primary (secondary) roads. All DEMs show a greater GA in hilly area (Milolé) than in flat area (Okondja) and SRTM derived DEMs show a higher UA for secondary roads constraint. UA for the lower or equal category varies between 55.5 and 75.2% (63.9 and 91.7%) for primary (secondary) roads. The use of corrected aster DEMs increases initial ASTER UA from 0.1 to 18.8% depending on category and slope limitation. Despite a relatively high RMSE for slope grade, all of the DEMs tested were found to be consistent for consideration of maximum slope constraint aiming at sustainable road planning for forest logging in Central Africa. [less ▲]

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See detailVoice Impact of a Prolonged Reading Task at two Intensity Levels
Remacle, Angélique ULg; FINCK, Camille ULg; Roche, Anne et al

Poster (2011, June 02)

The purpose of this study is to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading. Fifty normophonic women undergo two sessions of voice loading in ... [more ▼]

The purpose of this study is to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading. Fifty normophonic women undergo two sessions of voice loading in which the required intensity level of voice varied: 60-65 dB(A) for the first session, and 70-75 dB(A) for the second session. Serial voice quality objective data is obtained every 30 minutes during the oral reading tasks, by use of Multi-Dimensional Voice program (Average fundamental frequency (AvF0), Jitter percent (Jitt), Shimmer percent (Shim), Noise-to-Harmonic Ratio (NHR)). Serial measurements of voice range are also carried out every 30 minutes of the oral reading tasks, by use of the voice range profile. Results indicate that AvF0, lowest frequency of voice range and softest output of phonation rise during prolonged oral reading. On the contrary, Jitt tends to decrease and Shim decreases significantly with the passage of time during the voice loading. NRH does not vary statistically. AvF0 and highest outpout of phonation are the only parameters influenced by the required intensity of oral reading task: they are significantly higher in the 70-75 dB session compared to the 60-65 dB session. [less ▲]

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See detailSpecific contribution of short-term memory for serial order information to early reading acquisition: A longitudinal study
Martinez Perez, Trecy ULg; Majerus, Steve ULg; Poncelet, Martine ULg

Poster (2011, June 02)

Early reading acquisition skills have been linked to verbal short-term memory (STM) capacity. However, the nature of this relationship remains controversial. Here we distinguished between STM for item and ... [more ▼]

Early reading acquisition skills have been linked to verbal short-term memory (STM) capacity. However, the nature of this relationship remains controversial. Here we distinguished between STM for item and order information based on recent studies showing that STM for order is an important and independent predictor of oral language development. Tasks maximizing STM for serial-order or item information and reading tests were administered to 42 children from kindergarten through 1st grade. Results showed that order STM capacity measured at kindergarten predicted phonological recoding abilities at 1st grade. Implications of poor serial-order STM for reading acquisition in dyslexia are discussed. [less ▲]

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See detailInfluence of thermoelectric coupling on pacemaker activity generated by mechano-electric feedback in a one-dimensional ring-shaped model of cardiac fiber
Collet, Arnaud ULg; Desaive, Thomas ULg; Pierard, Luc ULg et al

Poster (2011, June 01)

The mechano-electric feedback (MEF) in the heart consists in the influence of the tissue deformations on the cardiac electrical activity. Under certain conditions, tissue deformations can generate ... [more ▼]

The mechano-electric feedback (MEF) in the heart consists in the influence of the tissue deformations on the cardiac electrical activity. Under certain conditions, tissue deformations can generate electrical perturbations via stretch-activated channels, such that the membrane potential can exceed the threshold value needed in order to trigger cardiac action potentials (APs). In the present study, we have developed a one-dimensional ring-shaped model of cardiac fiber taking into account three different couplings: the excitation-contraction coupling (ECC), the MEF and the thermoelectric coupling (TEC). The main goal of this work is to examine the effects of the TEC on the different properties of the pacemaker activity generated by the MEF. [less ▲]

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See detailTowards the understanding of the structure and assembly of the E. coli septum
Derouaux, Adeline ULg; Vollmer, Waldemar

Poster (2011, June)

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See detailKynurenic acid is able to suppress cortical spreading depression and the effect size is sex hormone dependent
Chauvel, Virginie ULg; Vamos, Eniko; Pardutz, Arpat et al

Poster (2011, June)

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See detailTranscriptomic analysis of Arabidopsis roots during floral induction by photoperiod
D'Aloia, Maria ULg; Lamoureux, Thibaut ULg; Tocquin, Pierre ULg et al

Poster (2011, June)

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an ... [more ▼]

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an hydroponic device that allows synchronous growth and flowering of Arabidopsis and performed global transcript profiling of roots. Samples were harvested during the extension period of a single long day (LD), and in non inductive short day. Microarray data were validated by real-time RT-PCR, and the expression patterns of selected probes were further analyzed in shoots and roots. Some of the genes that were differentially expressed in the roots during the inductive LD did not show the same variations in the shoot, indicating that root transcriptome undergoes specific changes at floral transition. These genes include, for example, GIGANTEA. T-DNA mutants from selected candidate genes are being studied. Both the expression analysis and the reverse genetic approach provide new insights into the contribution of the roots to the flowering process. [less ▲]

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See detailModeling post-combustion CO2 capture with amine solvents
Léonard, Grégoire ULg; Heyen, Georges ULg

Poster (2011, June)

Carbon capture and storage is a technology that can contribute to face the challenge of rising energy demand combined with a growing environmental awareness. In the present work, the CO2 capture process ... [more ▼]

Carbon capture and storage is a technology that can contribute to face the challenge of rising energy demand combined with a growing environmental awareness. In the present work, the CO2 capture process with monoethanolamine (MEA) is modeled using the simulation tool Aspen Plus. Two different modeling approaches are studied and compared: the equilibrium and the rate-based approaches. An optimization of key process parameters is performed and process modifications are studied with the objective of improving the global process energy efficiency. [less ▲]

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See detailCOMPARATIVE STUDY OF SPR BIOSENSORS BASED GOLD AND SILVER COLLOIDAL NANOPARTICLES
Lismont, Marjorie ULg; Dreesen, Laurent ULg

Poster (2011, June)

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which ... [more ▼]

Noble metal nanoparticles (NPs) can be used as a robust tool for optical bio-sensing. These NPs are known for their strong interactions with light through their surface plasmon resonance (SPR), which corresponds to the collective oscillations of the conduction electrons on the particles [1]. Among metals, silver and gold NPs are of particular interest not only because they are air-stable but also because their SPR absorption bands are in the visible and near ultra-violet spectral regions, that appear as the most appropriate for technological applications [2]. The first advantage of such optical SPR biosensors is their ability to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Because of the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. Biotin is a water-soluble B complex vitamin necessary for the production of fatty acids and the metabolism of fats and amino acids. The avidin is a tetrameric protein which can react with biotin to form the strongly bonded biotin-avidin complex.The prototypical biotin-avidin interaction forms the basis of a simple sol-based diagnostic technique for biological analytes. We focused on this well-known couple of bio-molecules to compare optical properties of silver and gold colloidal NPs. Gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs were studied as a function of added avidin. After avidin addition, an increased red-shift of the SPR wavelength and a broadening of the absorption band with time are observed. These changes in the optical properties of colloidal NPs are due to the biomolecular recognition process between biotin and avidin which leads to aggregation of these NPs arising from cross-linking by the tetrameric protein. Moreover, the recognition process induces a variation of the local refractive index around these NPs and thus induces a red-shift of SPR also. The maximum SPR red-shift was reached after 45 minutes and was equal to 25 nm and 15 nm for silver NPs and gold NPs respectively. We concluded that the dielectric sensitivity of gold NPs is smaller than the silver NPs one for a same geometry and for an equivalent concentration of avidin. Therefore, the silver sol is more adapted to detection of avidin than the gold sol. The detection limit, described as the lowest concentration for clear identification of wavelength shift [4] due to biomolecular recognition is determined to be 4 nM for both silver and gold NPs. In this case, the corresponding wavelength shift is about 3 nm. The specificity of the interaction between biocytin and avidin was checked by replacing avidin by BSA. When BSA was added, we observed a SPR shift which was smaller than the detection limit of 3 nm. Future works will be devoted to transpose this kind of biomolecular recognition experiments on gold nanorods in order to improve the dynamic phototherapy efficiency of cancers. [less ▲]

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See detailDiallyl disulfides: comparison between classical and microwave assisted synthesis
DETHIER, Bérénice ULg; Richel, Aurore ULg; Laurent, Pascal ULg et al

Poster (2011, June)

Garlic contains organosulfur compounds, such as diallyl disulfides (DADS), diallyl monosulfides (DAMS) and diallyl trisulfides (DATS), which have potential health properties. The first objective of this ... [more ▼]

Garlic contains organosulfur compounds, such as diallyl disulfides (DADS), diallyl monosulfides (DAMS) and diallyl trisulfides (DATS), which have potential health properties. The first objective of this work is to conduct the synthesis of these molecules by heating in a classical oil bath or by microwave irradiation with a phase transfer catalyst. We concluded that the synthesis should be cnducted at 40°C in an oil bath, with a phase transfer catalyst. [less ▲]

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See detailA cytokinin route to flowering in Arabidopsis
Bouché, Frédéric ULg; André, Julie ULg; D'Aloia, Maria ULg et al

Poster (2011, June)

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes ... [more ▼]

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes flowering in non-inductive short days. The response to BAP treatment does no require FLOWERING LOCUS T (FT), but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) (D'Aloia et al., 2011). We present here complementary data obtained with transgenic plants overexpressing a catalytic CK OXIDASE/DEHYDROGENASE (CKX) in the roots. The high efficiency of BAP in promoting flowering in our experimental system contrasts with the variability that emerges from studies gathered in literature. Many factors, either experimental or inherent to plant material, might explain these discrepancies and we are interested in identifying endogenous regulators that might provide a mechanistic explanation. We are therefore investigating whether the endogenous pathways underlying plant developmental phase changes might regulate the relative contribution of CKs to flowering. [less ▲]

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See detailAn easy-to-use pipeline for creating connectomes
Ziegler, Erik ULg; Foret, Ariane; Matarazzo, Luca et al

Poster (2011, June)

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See detailDefining Antonymy by studying Co-occurrences in Context
Steffens, Marie ULg

Poster (2011, June)

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See detailThe perceptual and verbal analogical reasoning in children with specific language impairment (SLI)
Leroy, Sandrine ULg; Guénébaud, Mélanie; Parisse, Christophe et al

Poster (2011, June)

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See detailMolecular histology for epithelial ovarian cancers biomarker hunting: new issues for biology and pharmacology.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, June)

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See detailEffect of blue monochromatic light on non-visual functions in aging
Daneault, V; Vandewalle, Gilles ULg; Teikari, P et al

Poster (2011, June)

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See detailAnt-aphid mutualism - How do ants locate aphid colonies?
Fischer, Christophe ULg; Sablon, Ludovic ULg; Duriaux, Adrien ULg et al

Poster (2011, June)

That some ant species show mutualistic relationships with some aphid species is well known since several decades. Ants protect aphids against natural enemies and as a reward collect the sugar-rich aphid ... [more ▼]

That some ant species show mutualistic relationships with some aphid species is well known since several decades. Ants protect aphids against natural enemies and as a reward collect the sugar-rich aphid excretion called honeydew. However, very little information is available on possible semiochemicals involved in these interactions. This study aims to identify semiochemicals involved in the establishment of this relation, using both chemical and behavioral approaches. Preliminary choice tests confirmed that ant scouts are able to detect aphid colonies at a distance using volatile organic compounds (VOC). Those VOC influencing ant’s orientation may originate from different sources: the attacked plant, the honeydew produced by the aphids and the aphids themselves. VOC emission profiles of each of those potential sources have been established using gas chromatography. Y-olfactometer assay revealed that honeydew volatile compounds are the key element in this distant attraction. Moreover, based on these VOCs, ant scouts are able to discriminate between the honeydew produced by a myrmecophilous and a non myrmecophilous aphid species. Thus, more than just a sugar source, honeydew is an attractant for ant scouts towards myrmecophilous aphids. [less ▲]

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See detailOn generalized Hölder spaces
Kreit, Damien ULg; Nicolay, Samuel ULg

Poster (2011, June)

The Hölder spaces provide a natural way for measuring the smoothness of a function. These spaces appear in different areas such as approximation theory and multifractal analysis and lead to natural ... [more ▼]

The Hölder spaces provide a natural way for measuring the smoothness of a function. These spaces appear in different areas such as approximation theory and multifractal analysis and lead to natural definitions of the notion of fractal function; for example a function belonging to $C^\alpha$ with $\alpha\in (0,1)$ typically has a fractal graph. The purpose of this poster is to present a generalization of such spaces as well as some recent results about their characterizations. [less ▲]

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See detailStructure-Surface Activity Relationships Of Uronic Acid-Derivative Surfactants From Renewable Resources Applications
Razafindralambo, Hary ULg; Richel, Aurore ULg; Richard, Gaetan ULg et al

Poster (2011, June)

Introduction and Background: Uronic acid-derivative surfactants are amphiphilic compounds with growing interests owing to their potential applications in various areas [1]. This class of carbohydrate ... [more ▼]

Introduction and Background: Uronic acid-derivative surfactants are amphiphilic compounds with growing interests owing to their potential applications in various areas [1]. This class of carbohydrate-based surfactants can be easily prepared from renewable raw materials in a wide range of structure by modular synthesis thanks to the presence of one carboxylic and numerous hydroxyl groups. The polar head group configurations according to the stereochemistry of OH groups, and geometry (cyclic or not), the hydrophobic tail (number and length of alkyl chain), and the type of linker (ester, acetal and amide, etc) are among the main variables in their structural entities. Therefore, the investigation of their structure-surface activity relationships appears valuable for increasing backgrounds, and achieving a rational design for selecting the best structures to be used in different industrial fields [2]. Methodology: A few analogous of glucuronic acid-derivative surfactants have been synthesized by chemical or enzymatic routes. After purification, their structure has been confirmed by various spectroscopic techniques (RMN, MS, IR). Surface tensions of true aqueous solution have been then measured in dynamic and static modes using a series of complementary techniques. Critical micelle concentrations, minimum molecular areas, and maximum surface excesses have also been determined. Results and Discussion: Glucuronic acid derivative surfactants under investigation vary in the polar head group configuration including cyclic or non-cyclic structure and α or β anomeric form, in the hydrophobic tail regarding to the chain length (C8 to C14), the presence of a double bond, as well as an OH group at the terminal carbon, and in the type of linker, ester in C6 or acetal in C1. Results showed that all of these structural attributes affect both dynamic and equilibrium surface properties of glucuronic acid -based surfactants. Conclusion: A set of synthetic glucuronic acid-based surfactants varying in the polar head group, hydrophobic tail, and linker allow us to generate various surface-active properties at the air-water interface, and to increase the knowledge on relationships between their structure and surface-active properties. [1] Laurent, P.; Razafindralambo, H.; Wathelet, B.; Blecker, C.; Wathelet, J.-P.; Paquot, M., Synthesis and Surface-Active Properties of Uronic Amide Derivatives, Surfactants from Renewable Organic Raw Materials. Journal of Surfactants and Detergents 2010, in press. [2] Razafindralambo, H.; Blecker, C.; Mezdour, S.; Deroanne, C.; Crowet, J.; Brasseur, R.; Lins, L.; Paquot, M., Impacts of the Carbonyl Group Location of Ester Bond on Interfacial Properties of Sugar-Based Surfactants: Experimental and Computational Evidences. The Journal of Physical Chemistry B 2009, 113 (26), 8872-8877. Acknowledgment: This work was supported by Belgian Walloon Region within DGTRE research project of excellence (TECHNOSE). [less ▲]

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See detailFunctional and physicochemical properties of starches isolated from pearl millet landraces grown in hyperarid regions
Boudries, Nadia; Sindic, Marianne ULg; Nadjemi, Boubekeur et al

Poster (2011, May 31)

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See detailKraft RB : recurrent neural network prediction of steam production
Sainlez, Matthieu ULg; Heyen, Georges ULg

Poster (2011, May 30)

In this study, neural networks approaches are compared for predicting the high pressure (HP) steam flow rate from a Kraft recovery boiler. We apply two types of neural networks: a static multilayer ... [more ▼]

In this study, neural networks approaches are compared for predicting the high pressure (HP) steam flow rate from a Kraft recovery boiler. We apply two types of neural networks: a static multilayer perceptron and a dynamic Elman’s recurrent neural network. Starting from a one-day database of raw process data related to the boiler, the goal is to model and predict the next 12-hours of HP steam flow production from the boiler to the steam turbine. The results illustrate the potential of the dynamic approach in this task. [less ▲]

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See detailRelations between a computerized shopping task and cognitive and clinical variables in patients diagnosed with bipolar disorder and alcohol dependency compared with healthy controls.
Laloyaux, Julien ULg; Michel, Céline; Pellegrini, Nadia et al

Poster (2011, May 27)

Persons diagnosed with bipolar disorder and alcohol dependency are frequently subject to poor everyday life functioning. However, previous studies have primarily used questionnaires or observational ... [more ▼]

Persons diagnosed with bipolar disorder and alcohol dependency are frequently subject to poor everyday life functioning. However, previous studies have primarily used questionnaires or observational methods to assess everyday life functioning, both of which contain a number of limits. In order to address some of these limits, we developed a computerised real-life activity task, in particular, a shopping task where participants are required to shop for a list of 8 grocery store items. Twenty individuals diagnosed with alcoholic dependence and 21 patients diagnosed with bipolar disorder were compared with 20 and 21 matched healthy controls, respectively. All participants completed the shopping task, and both clinical groups were evaluated with an extensive battery of neuropsychological tests and a measure of global functioning. Results showed that, for both clinical groups, performance on the computerised shopping task significantly differentiated patients and healthy controls for a number of variables, especially total time and mean time to consult the shopping list. Performances on shopping task variables, in both clinical groups, were also significantly correlated with neuropsychological tests measuring verbal episodic memory, processing speed and selective attention. Finally, performances on the computerised shopping task were significantly correlated with various clinical variables and with global functioning in both patient groups. These findings suggest that the computerised task used in the present study provides a valid indication of the level of everyday life functioning for these clinical populations, and therefore may be viewed as a valuable instrument in both an evaluation and remediation context. [less ▲]

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See detailEfficacy of errorless learning in the acquisition of a new procedural skill in Alzheimer's disease
Schmitz, Xavier ULg; Lejeune, Caroline ULg; Vervecken, Nancy et al

Poster (2011, May 27)

In Alzheimer’s disease (AD), implicit or procedural rehabilitation techniques would be more effective to train new skills than explicit or declarative learning methods (van Halteren-van Tilborg, 2007 ... [more ▼]

In Alzheimer’s disease (AD), implicit or procedural rehabilitation techniques would be more effective to train new skills than explicit or declarative learning methods (van Halteren-van Tilborg, 2007). Following Baddeley and Wilson (1994)’s assumption, Maxwell et al. (2001) showed that reducing errors during motor learning minimizes the building of declarative knowledge and would allow implicit knowledge accumulation. However, most studies on errorless learning focused on learning of face-name associations (Clare et al., , 2001), and very few studies have investigated errorless learning in procedural learning situations, even though some data suggest that errorless learning would be efficient for learning instrumental activities of daily living (e.g., Thivierge et al., 2008). The aim of this study was to examine the efficacy of an error-reducing versus an errorfull method in motor skill learning. We examined the acquisition of a new motor skill in 24 patients with AD and 24 healthy older adults matched for age, sex, and education. In this task, subjects had to follow with a reversed mouse the contour of a form (a star) displayed on a computer screen. Half the subjects learned in an error-reducing condition, and the others in an errorfull condition. After the learning phase, all the subjects had to complete a novel form. Results show an advantage for the error-reducing condition in the AD group, whereas the performance of the healthy participants did not differ between the two conditions, confirming the efficiency of errorless learning principles in AD for procedural learning situations. [less ▲]

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See detailThe importance of short-term memory for order in dissociating short-term memory and language deficits
Attout, Lucie ULg; VAN DER KAA, Marie-Anne ULg; GEORGE, Mercédès ULg et al

Poster (2011, May 27)

Selective verbal short-term memory (STM) deficits are rare, and when they appear, they are often associated with a history of aphasia, raising doubts about the selectivity of these deficits. We explore ... [more ▼]

Selective verbal short-term memory (STM) deficits are rare, and when they appear, they are often associated with a history of aphasia, raising doubts about the selectivity of these deficits. We explore here the distinction between STM for item information and STM for order information to separate STM and language impairments. Recent models of STM consider that STM for item information depends upon activation of the language system, and hence item STM deficits should be associated with language impairment. By contrast, STM for order information is considered to recruit a specific system, distinct from the language system. In this view, order STM should be impaired in patients with STM deficits that cannot be accounted for by language impairment. We applied this rationale to the exploration of STM profiles of patients MB and CG. Patient MB showed mild phonological impairment and associated STM deficits. As predicted, these were characterized by poor item STM but preserved order STM. Patient CG showed verbal STM deficits with no associated language deficits. His STM deficit was characterized by poor order STM but relatively preserved item STM. This study presents the first double dissociation between item and order STM deficits, demonstrating the necessity of this distinction for understanding selective STM impairment. [less ▲]

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See detailEmergency physicians’ communication: What about a standardized handover?
Gillet, Aline ULg; D'ORIO, Vincenzo ULg; Nyssen, Anne-Sophie ULg

Poster (2011, May 27)

In health care organizations, the need for 24-hour care increases the number of patient’s transfer. Handover and shift changes are now recognized as particularly critical moments for the reliability of ... [more ▼]

In health care organizations, the need for 24-hour care increases the number of patient’s transfer. Handover and shift changes are now recognized as particularly critical moments for the reliability of care. However, few studies focused specifically on how physicians share relevant information during these moments. Moreover, emergency departments are known to be extremely vulnerable to error, because of high time pressure, frequent interruptions, high variability and number of patients, etc. Our study aimed to estimate how emergency physicians share information about a patient during handover, and to evaluate the impact of a procedure of medical handover standardization. To do this, we conducted a pre-post test on completeness of transmissions. We first performed observations of 50 physicians’ shift changes in an emergency department. All of these were video-recorded and then analyzed. We classified communications into seven categories (identifying information, current pathology, patient’s current status, significant patient’s tests results, priority medical interventions, diagnose and recommendations, and dispositions). After these observations, we elaborated and implemented a standardization procedure, according to literature, analyses of our observations and physicians’ recommendations. We finally assessed this procedure by observing and analyzing 40 handovers, using the same method as previously described. We also evaluated the physician’s opinion about the quality of the transmission with a 7-point Likert scale. Our results showed three significant differences before and after the standardization of communications. Physicians share more information about patient’s tests results, priority interventions and dispositions. Moreover, we found a significant difference of the perceived quality of the standardized handover. [less ▲]

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See detailDevelopmental differences in the procedural learning of a perceptual-motor skill
Lejeune, Caroline ULg; Catale, Corinne ULg; Merbah, Sarah ULg et al

Poster (2011, May 27)

It is generally admitted that procedural learning is efficient early in childhood. However, few studies have brought empirical data confirming this assumption, and many questions remain regarding the ... [more ▼]

It is generally admitted that procedural learning is efficient early in childhood. However, few studies have brought empirical data confirming this assumption, and many questions remain regarding the cognitive mechanisms that sustain procedural learning in children. The aim of our study was to investigate whether perceptual-motor procedural learning was present to the same extent in 7-, 10-year-old children and in adults. We also examined the role of executive functions, working memory, general intelligence, and motor ability during the learning process. A total of 76 subjects divided into 3 age-groups were tested. The task included 4 blocks of 3 trials during which each subject had to trace the contour of a triangle with an inverted computer mouse. Analyses show an important difference between groups in the initial phase of the learning process. They also reveal that executive functions intervene during the first learning phase, which might explain the observed age effect. In addition, results show significant but different learning effects for the procedural task: while the improvement was equivalent between 10-year-olds and adults, 7-year-old children showed a greater learning slope than the other groups; despite their slowness during the first blocks, younger children showed an equivalent performance at the end of the learning phase. These results suggest that, if executive processes are important during the first learning steps, they are not a “necessary condition” for motor skill learning to occur. The role of compensatory strategies sustaining learning in younger children is discussed. [less ▲]

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See detailChildren and parents: The anxiety sensitivity, what links?
Stassart, Céline ULg; Etienne, Anne-Marie ULg

Poster (2011, May 27)

Objectives. Anxiety sensitivity (AS) is the fear of anxiety-related sensations. This work examines the relationships between childhood learning experiences and the development of AS, in a non-clinical ... [more ▼]

Objectives. Anxiety sensitivity (AS) is the fear of anxiety-related sensations. This work examines the relationships between childhood learning experiences and the development of AS, in a non-clinical sample of children. Methods. Seventy normal children aged 9 to 12 years were interviewed about their learning experiences with anxiety-related and cold-related somatic symptoms. Three learning status were examined: -reinforcement, verbal transmission and observational (mother and father) learning- using the Questionnaire d'Expérience d'Apprentissage (QEA). AS levels were assessed using the Childhood Anxiety Sensitivity Index (CASI). Results. Correlations between QEA and CASI (r between 0.31 and 0.60, p<0.01) are significant, except for the reinforcement of somatic symptoms in learning by observation of the father. For boys, all learning experiences correlate with CASI scores (r between 0.33 and 0.62, p<0.001), except the reinforcement in learning by observation of the father. For girls, two correlations are observed: for verbal transmission learning following anxiety (r = 0.57) and cold (r = 0.55) symptoms (p=0.001). The level of AS correlate with learning by observation of the father only for boys (r=0.38, p<0.05). Conclusions. These results could suggest that learning experiences seem to play a significant role in the development of AS and suggest also a gender transmission: between different status of learning for boys and girls and between the observation of the mother and the father. Learning by observation of the father will only be linked to boy's AS. These findings bring to question the specific impact of a father's fears in the development of children's AS. [less ▲]

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See detailPsychology of migration and losses: Mourning process in families native from Democratic Republic of Congo
Thiltges, Esther ULg; D'Amore, Salvatore ULg; Blavier, Adelaïde ULg

Poster (2011, May 27)

Migration constitutes a potentially traumatic event along with cultural losses and psychic reorganizations (Moro, 1994, 1998). The lost object is physically absent but psychologically present and this ... [more ▼]

Migration constitutes a potentially traumatic event along with cultural losses and psychic reorganizations (Moro, 1994, 1998). The lost object is physically absent but psychologically present and this ambiguity precludes the mourning process (Boss, 1999). Indeed migration losses are unclear, incomplete and potentially retrievable (ex: return in the country, Falicov, 2003). The mourning process cannot be achieved because the object (country, family, cultural and social environments) is still existing (Aouattah, 2003). Nevertheless, exile does not have to be seen only as traumatizing but also as a potentially creative experience (Douville, 2001). Therefore, we also took into account benefits of the migration and individual resources. We analyzed a sample of five families from the Democratic Republic of Congo – with open questions during two meetings – and we tested the following hypothesis: "the mourning process is the result of the dynamic interactions between losses, resources and benefits ". We defined these dynamics through three steps: association and confusion; dissociation and identification; integration and achievement of the mourning process. We tested our hypothesis using Alceste – which is a statistical tool for discourse analysis – and the results confirmed it. We can state our conclusions as follows. At the first stage of the process, there exists confusion between losses, resources and benefits. At the second stage, the persons can differentiate and separate these three elements. And at the last step, people can achieve the mourning process, live with their losses and launch themselves into the future. [less ▲]

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See detailThe "other-age" effect in age estimation of faces
Moyse, Evelyne ULg; Manard, Marine ULg; Brédart, Serge ULg

Poster (2011, May 27)

In this study the occurrence of an “other-age” effect on age estimation was assessed. This effect consists in a more accurate estimation of own-age faces than other-age faces. Here, the age of ... [more ▼]

In this study the occurrence of an “other-age” effect on age estimation was assessed. This effect consists in a more accurate estimation of own-age faces than other-age faces. Here, the age of participants (10 to 14 year old children and 20 to 30 year old adults) and the age of stimuli (faces of 10 to 14 year old children and faces of 20 to 30 year old adults) were crossed. Although we did not observe a crossed interaction where each age group would have been more accurate for in-group estimation than for out-group estimation, present results revealed the occurrence of an own-age bias in age estimation. Although both groups did not differ when estimating child faces, adults were more accurate than children when estimating the age of adult faces. Therefore, the present results showed an asymmetrical "other-age" effect. Several hypotheses explaining the present pattern of results are discussed. [less ▲]

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See detailLexical and inflectional spelling abilities in French : Same or different ?
Binamé, Florence ULg; Poncelet, Martine ULg

Poster (2011, May 27)

Inflectional spelling abilities have been less extensively studied than lexical spelling abilities and the relationship between these two types of spelling abilities is poorly understood. In this study ... [more ▼]

Inflectional spelling abilities have been less extensively studied than lexical spelling abilities and the relationship between these two types of spelling abilities is poorly understood. In this study, we compared lexical and inflectional spelling abilities in 12-year-old children, by hypothesizing that attentional load may be particularly detrimental for sentence context-dependent inflectional spelling abilities, in contrast to sentence context-independent lexical spelling abilities. Ninety-five sixth graders had to write to dictation sentences containing orthographically inconsistent words as well as inflected words (plural nouns and verbs at the 3th person) in three different cognitive load conditions. In a medium load condition, a time constraint obliged the subjects to write the sentences as quickly as possible. In a high load condition, the participants had to write the sentences as quickly as possible while further carrying out calculation problems (additions). The minimal load condition corresponded to standard writing to dictation, with no time constraint or any secondary task. The number of spelling errors for orthographically inconsistent words (lexical spelling abilities) and inflected words (inflectional spelling abilities) was calculated for each condition. We observed that spelling performance for inconsistent words was not impacted by cognitive load. On the other hand, spelling performance for inflected words decreased significantly in the medium and high load conditions. These results suggest that inflectional spelling abilities are less proceduralized than lexical spelling abilities, and rely to a greater extent on cognitive control processes. [less ▲]

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See detailOpinions and practice of forensic psychologists about credibility assessment
Beerten, Jérôme ULg; Blavier, Adelaïde ULg

Poster (2011, May 27)

Assessing victim’s credibility is essential but very complex when physical evidence is lacking. The dual query of evaluating victim’s credibility and sequels and unclear mandates increase the confusion ... [more ▼]

Assessing victim’s credibility is essential but very complex when physical evidence is lacking. The dual query of evaluating victim’s credibility and sequels and unclear mandates increase the confusion. Similarly, the use of the controversial term “credibility” illustrates the different expectations of the various protagonists involved in the judicial process. The conditions of evaluation and the limitations of available methods and assessment tools make this task difficult for the forensic psychologists. In this context, our exploratory study aimed to understand how forensic psychologists actually performed their task. In 2007, we interviewed 11 forensic psychologists with a questionnaire on their training, their practice, their use of the different methods of assessment, their understanding of the concept of credibility, their perception of their role and the content of their forensic report. Different practices were observed, although a majority of forensic psychologists used the Statement Validity Analysis (SVA) and seemed to use the assessment tools within the limits of their duties. Despite the use of scientifically-validated tools, they gave a great attention and value to nonverbal behaviors and symptoms in their evaluation. Moreover, 5/11 forensic psychologists considered transmission of data about the reality and/or truth of the facts was an integral part of their mission. Another sample of 16 forensic reports we analyzed in parallel showed under-utilization of scientifically valid assessment tools of suggestibility and an overuse of SVA elements (e.g., without video-tape-recording). In conclusion, forensic psychologists seemed to support their conclusions more on a range of consistent elements than on scientifically-based salient information. [less ▲]

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See detailImpact of visual modality on acoustic assessment of rooms
Defays, Aurore ULg; Safin, Stéphane ULg; Billon, Alexis ULg et al

Poster (2011, May 27)

In the context of the conception of a multimodal immersive environment aimed to support the task of assessing the acoustical quality of rooms based on numerical simulations, we study the relationship ... [more ▼]

In the context of the conception of a multimodal immersive environment aimed to support the task of assessing the acoustical quality of rooms based on numerical simulations, we study the relationship between image and sound. Auralias project aims to develop an immersive environment for a collaborative work between architects and acousticians combining 3D sound rendering and 3D view of the room model. In this study, we analyzed the impact of a room picture on the acoustic assessment. We chose reverberation as acoustic parameter. First, we confronted 70 subjects with a sound clip and asked them to assess the reverberation degree (control condition). Then, we divided the subjects into three groups and repeated the same judgment task. However, in the first group, subjects listening the sound clip were confronted with a room picture consistent with the sound. In the second group, they were confronted with a « distractor » room picture (no link with the sound). In the last group, they listened the sound clip without any room picture. Our results showed a positive impact of consistent pictures on reverberation assessment but we found no effect of distractor pictures. [less ▲]

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See detailReactive Attachment Disorder and socio-emotional development in childhood: Clinical review.
Wertz, Céline ULg; Gauthier, Jean-Marie ULg; Blavier, Adelaïde ULg

Poster (2011, May 27)

The quality of interactions experienced with primary attachment figures influence the development of emotional skills. On the other hand, we know how emotions fill a critical adaptive role for the social ... [more ▼]

The quality of interactions experienced with primary attachment figures influence the development of emotional skills. On the other hand, we know how emotions fill a critical adaptive role for the social adjustment, in that they assume both a communicative function and an informative value. In this research, we were particularly interested in how children’s patterns of attachment were expressed in terms of emotional regulation abilities. According to Laible & Thompson’s observations (1998), we tested the following hypothesis: insecure attachment representations are associated with a poverty of skills in decoding emotional signals. They especially would affect the perception of negative emotional expressions. We tested this hypothesis by the meeting of five children of primary school age (5 to 8 years old) with a reactive attachment disorder and through the establishment of two methodological tools. At first, the Attachment Story Completion Task (Bretherton et al., 1990) allowed us to identify attachment representations for each child. Secondly, inspired by Pollak & al.’s study, we developed a recognition task of facial emotional expressions. We observed in these children low average rates of identification of basic and primary emotions. Especially, the accuracy of judgments was not only a function of emotion’s valence, but was also dependant of the child’s attachment pattern. Finally, this research confirmed the observations, already showed in previous studies, that interpersonal difficulties presented by these children could be explained specifically by their inefficiency in interpreting social cues surrounding emotional events (Crick & Dodge, 1994). [less ▲]

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See detailHow conditioning by one’s job leads to visual misrepresentation: Evidence from Rorschach test in nurse population
Englebert, Jérôme ULg; Blavier, Adelaïde ULg

Poster (2011, May 27)

Exner (1974, 2003, 2005) has developed an empirical method for analyzing answers at Rorschach test. This analysis is not focused on the answer content but more on perception, information processing and ... [more ▼]

Exner (1974, 2003, 2005) has developed an empirical method for analyzing answers at Rorschach test. This analysis is not focused on the answer content but more on perception, information processing and quality of the answer. Thus, contrary to psychoanalysis, the content and its interpretation are not systematically studied in this empirical approach. In this perspective, our purpose was to empirically study how answers and performance at Rorschach test were conditioning by one’s job. We administrated Rorschach test to 38 nurses and 38 paired subjects (matched for gender and age). Our data showed nurses gave significantly more anatomical answers than control subjects, this first result confirmed a clinical assumption that was never objectified by previous studies: conditioning by one’s job influences the answer content at the Rorschach test (e.g., people from medical sector tend to see organs). The second main result is the formal qualities of anatomical answers given by nurses were significantly worse and more unusual than anatomical answers given by the control group. These findings suggest that conditioning by one’s job is so strong that it overrides the other choices of answers and can lead to a deformation of the visual perception. Furthermore, the content is the main answer element subjects can control in this test and thus, it could be a means of affirming his/her identity (e.g., nurse’s identity by anatomical content). Moreover, in regard to the bad formal quality of anatomical answers, it seems that nurse’s identity takes precedence over the reality and the actual form perception. [less ▲]

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See detailQuantification and monitoring of contaminant mass fluxes in heterogeneous subsurface media
Jamin, Pierre ULg; Brouyère, Serge ULg

Poster (2011, May 26)

Since the beginning of the years 2000, a consensus has been growing among the scientific, technical and decision makers community concerning the fact that the characterisation and the management of ... [more ▼]

Since the beginning of the years 2000, a consensus has been growing among the scientific, technical and decision makers community concerning the fact that the characterisation and the management of contaminated sites have to be performed in terms of contaminant flux metrics instead of to be based on a simplistic study of pollutant concentration coming from piezometers randomly spread over the site. Contaminant plumes and associated pollutant mass fluxes are extremely variable in space as well as in time. The scientific and technical challenge is to capture this double heterogeneity in order to consider it explicitly or statistically for the management of a contamination issue. This requires (1) the interception of the entire contaminated groundwater flowing section with a control panel; (2) the ability to measure and to calculate accurately groundwater and contaminant fluxes across that panel; and (3) the repetition of the measurement to bring out the spatial and the temporal variation of contaminant fluxes. This research project aims to develop an integrated pollutant flux measurement approach for contaminated groundwater at the scale of the contaminant plume. This approach lays on a triple integration of (1) single well tracing techniques – (2) passive sampling – (3) control panel. Spatial and temporal variability of groundwater fluxes will be quantified by the point dilution tracing method derived from the “FVPDM” technique (Brouyère et al. 2008). In the same time, the amount of pollutant flowing through the same location will be determined by the adequate passive sampling technique (PS). The integration of these two measures in an interpretation schema based on mathematical and numerical modelling will allow quantifying contaminant fluxes and their variability over time. The adaptation at the scale of the contaminant plume will be based on a logging-type use of techniques, repeated along the vertical axis of the monitoring wells and repeated from one well to an other of a control panel implanted to intercept the plume. All the research results and developed techniques will then be integrated into a complete measurement system (PS-FVPDM), mobile and easily usable on field. The whole development and testing will take place on several pilot contaminated test site located in various hydrogeological context. [less ▲]

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See detailPerformance of leaf wetness sensor used in winter wheat disease management
Mahtour, Abdeslam ULg; El Jarroudi, Moussa ULg; Hoffmann, Lucien et al

Poster (2011, May 26)

Wetness on crop leaves has particular epidemiological significance because many fungal diseases affect plants only when free moisture is present on leaves. The leaf wetness sensor detects the presence of ... [more ▼]

Wetness on crop leaves has particular epidemiological significance because many fungal diseases affect plants only when free moisture is present on leaves. The leaf wetness sensor detects the presence of wetness on a leaf’s surface, enabling researchers and producers to forecast disease and protect plant canopies, and consequently to optimize fungicide application and often reduce environmental load. This research project aimed at better understanding the leaf wetness duration and its influence in winter wheat disease. Measurement of surface wetness duration by three electronic flat-plate sensors (Model 237-Campbell Scientific, Inc) in wheat fields were compared with tactile and visual observations in replicated field experiments at the site of Arlon (Belgium) during the period May-July 2006 and April-July 2007. Performances of the sensor were evaluated against SWEB model outputs and visual observations of disease symptoms. On the field, dew-onset and dry-off of wetness on leaves were observed visually (with a flash light for dew-onset) at 15-minute intervals. Each sensor was placed close the flag leaf. For the three sensors, the two dew-onset and dry-off times measured in both 2006 and 2007 crop seasons gave a leaf wetness duration (LWD) which was on average one hour less than visual observations. In order to establish a relationship between the surface wetness periods and wheat foliar diseases, LWD was compared with the Septoria leaf blotch (SLB) development risk (main winter wheat disease). A minimal surface wetness duration favourable to infection for SLB was established. [less ▲]

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See detailCompetency Model validation process : an illustration in public administration sector
Lothe, Benoit ULg; Hansez, Isabelle ULg; Bertrand, Françoise ULg

Poster (2011, May 26)

Background: Nowadays Human Resource Management issues are highly related to the strategic approach (Analoui, 2007; Daft, 2008; Becker, Huselid & Beatty, 2009 ; Jackson, Schuler & Werner, 2009). Workforce ... [more ▼]

Background: Nowadays Human Resource Management issues are highly related to the strategic approach (Analoui, 2007; Daft, 2008; Becker, Huselid & Beatty, 2009 ; Jackson, Schuler & Werner, 2009). Workforce is considered as an asset and HRM is required to match human capital with organization strategic purposes. HRM practices have accordingly evolved to better fulfil this requirement through for instance Competency Modelling (CM) (Werbel & De Marie, 2005). Diagnosis: This communication illustrates a specific CM validation process in a wide Public Administration. Through a “one-size-fits-all” competency model approach (Mansfield, 1996), the HRM department of this Public Administration has developed a set of 29 transverse competencies broken down across 290 behavioural indicators. After several in-house pre-validation steps (workshops and test cases), Human Resources Development Unit of University of Liege was invited to collaborate to the final validation process. Solution: Based on Van Beirendonck (2006) CM validation criteria, a two steps validation method was jointly developed. Four main criteria were used, therefore each behavioural indicator ought to : (1) meet various organizational departments needs; (2) be clearly understood within the organization (Bowen & Ostroff, 2004); (3) have a straight link with its belonging competency and (4) be observable. A questionnaire survey (n=252) was drawn up to appraise the first three criteria and quantitative analysis results provided the CM improvement framework. The concluding validation stage assessed the three last criteria by measuring Subject Matter Experts (HR managers responsible for staff evaluation) interrater agreement in order to improve CM content validity (Sanchez & Levine, 2009). Finding: Results comfortably confirm CM content validation under the four criteria. One of the project’s more relevant elements is developing a scientifically based method adapted to wide administration organizational features and constraints. In contrast, this CM validation project presents some limitations in relation to the CM theoretical concept (ambiguous concept in its validation perspective) and to organizational constraints (lack of strategic project concerning the use of the CM). Method and outcomes will be discussed in connection with the customer’s requirements and the constraints from the organizational context. [less ▲]

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See detailPrevalence and screening of burnout among Belgian population
DE CIA, Julie ULg; Mairiaux, Philippe ULg; Schippers, Nathalie ULg et al

Poster (2011, May 26)

The aim of the study was to estimate the prevalence of burnout among Belgian workers through the identification of burnout cases within the practices of general practitioners (GP) and the health ... [more ▼]

The aim of the study was to estimate the prevalence of burnout among Belgian workers through the identification of burnout cases within the practices of general practitioners (GP) and the health surveillance carried out by occupational health physicians (OP) .The prevalence may be estimated at about 0,8 % of the Belgian population; this figure is similar among workers consulting GP and OP. [less ▲]

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See detailComparative study of gold and silver based nanobiosensors
Lismont, Marjorie ULg; Dreesen, Laurent ULg

Poster (2011, May 25)

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1 ... [more ▼]

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1]. Optical SPR biosensors are able to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Due to the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. In our study, the prototypical biocytin-avidin interaction was used to study gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs as a function of added avidin. After avidin addition, a SPR red-shift and a broadening of the SPR bands were observed with both NPs. These optical changes evolved with time and reached their final values after around 45 min for each system. The maximum SPR red-shifts were equal to 25 nm and 15 nm for silver NPs and gold NPs, respectively. The detection limit, described as the lowest concentration for clear identification of wavelength shift due to biomolecular recognition, is determined to be 4 nM for both silver and gold NPs. The specificity of the biocytin-avidin biosensors was checked by replacing avidin by BSA. When BSA was added, we observed a SPR band shift which was smaller than the detection limit of 3 nm, attesting the biosensor selectivity. Our work demonstrates the superiority of Ag over Au NPs for the elaboration of biosensors based on SPR. [less ▲]

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See detailMultiqubit symmetric states with a high geometric entanglement
Martin, John ULg; Giraud, Olivier; Braun, Peter et al

Poster (2011, May 25)

We propose a detailed study of the geometric entanglement properties of pure symmetric N-qubit states, focusing more particularly on the identification of symmetric states with a high geometric ... [more ▼]

We propose a detailed study of the geometric entanglement properties of pure symmetric N-qubit states, focusing more particularly on the identification of symmetric states with a high geometric entanglement and how their entanglement behaves asymptotically for large N. We show that much higher geometric entanglement with improved asymptotical behavior can be obtained in comparison with the highly entangled balanced Dicke states studied previously. We also derive an upper bound for the geometric measure of entanglement of symmetric states. The connection with the quantumness of a state is discussed. [less ▲]

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See detailEVALUATION FOR RESISTANCE TO APHIDS OF WHEAT GERMPLASM RESOURCES IN CHINA
Zhou, Haibo ULg

Poster (2011, May 24)

Host plant resistance plays important roles in controlling pests and protecting of natural enemies in an agroecosystem, and the effect of insect resistant varieties in reducing damage is conspicuous ... [more ▼]

Host plant resistance plays important roles in controlling pests and protecting of natural enemies in an agroecosystem, and the effect of insect resistant varieties in reducing damage is conspicuous. Fuzzy recognition technique evaluation were used to determine sources of resistance in wheat to populations of the wheat aphids in five field experiments over 2 years in wheat-producing areas of China. Results showed that the majority of wheat varieties identified were susceptible to aphids, and no immune and highly resistant varieties was observed. The mean percentage from the wheat varieties with resistant, lowly susceptible, moderately susceptible and highly susceptible to aphid were 9.30%, 23.15%, 42.32%, and 25.23% in whole wheat varieties respectively. 5 wheat varieties (Lantian18, Lantian20, Lantian22, Lantian00-30 and Shanmai175) were moderately resistant in Jiangyou in 2009, other resistant sources were low levels for aphid-resisant. And more importantly, 2 wheat varieties (Lantian20, Lantian22) with the continuous resistance to wheat aphid in the five experimental fields over 2 years were found. Although resistance of wheat varieties had a close relation to their genetics and inheritance, we also found that the evaluation result of the same wheat varieties were varied in different place. The results indicated that the resistance to aphid could be affected by geographical environment.These evaluations on reaction to aphid metapopulation that could be crucial when germplasm is used in crosses to improve wheat cultivars. This research is part of program to evaluate germplasm for useful traits and make this information available to the germplasm system. [less ▲]

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See detailA semiochemical enhancing the attractiveness of aphidophagous predators in potato crops
Leroy, Pascal ULg; Vandereycken, Axel ULg; Sabri, Ahmed ULg et al

Poster (2011, May 24)

Aphids are major pests of crops worldwide and the use of pesticides has led to resistant populations. The integration of aphid natural enemies in integrated management programs could be an option, but ... [more ▼]

Aphids are major pests of crops worldwide and the use of pesticides has led to resistant populations. The integration of aphid natural enemies in integrated management programs could be an option, but their efficacy is often limited by their quick dispersal from the ecosystem where they are released. Here, using wind-tunnel and field experiments, we have demonstrated that 3-Methyl-2-butenal acts as an efficient attractant and ovipositional stimulant for the hoverfly Episyrphus balteatus (De Geer) (Diptera: Syrphidae), enhancing its efficiency as biological control agent in crop fields. Wind-tunnel assays allowed determining that a minimal dose of 250 µg of 3-Methyl-2-butenal is required to attract the hoverflies over a distance of 2.5 m while a minimal dose of 500 µg is needed to induce the oviposition. Both attraction and oviposition increased proportionally to the tested doses showing that hoverflies are able to regulate their oviposition according to the chemical stimuli from their environment. In field experiments, both Syrphidae and Chrysopidae were strongly attracted by the 3-Methyl-2-butenal at a dose of 10 mg which also induces the oviposition of these two aphid predators in potato crops. This study also highlighted that hoverflies are the most abundant aphid predators found in natural environment. Against aphids, the use of this semiochemical could certainly enhance the efficiency of natural enemies. Furthermore, the use of the chemical cue 3-Methyl-2-butenal could provide a novel approach to control aphids in field and greenhouse systems. [less ▲]

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See detailVolatile organic compounds of the roots of barley and their role in the rhizosphere
Fiers, Marie ULg; Barsics, Fanny ULg; Camerman, Marc ULg et al

Poster (2011, May 24)

Volatile organic compounds emitted by plants are known to intervene with various biotic environmental factors. Up to now, most of the studies have been focused on aerial volatiles and root liquid exudates ... [more ▼]

Volatile organic compounds emitted by plants are known to intervene with various biotic environmental factors. Up to now, most of the studies have been focused on aerial volatiles and root liquid exudates. Very few researches have been completed concerning belowground volatiles released into the rhizosphere despite their potential capacity to carry information between organisms. The Rhizovol project, started in autumn 2010, involves 5 different units of Gembloux Agro-Bio Tech collectively studying the production of belowground volatiles by barley roots underlying various biotic interactions in the rhizosphere. Some preliminary results of each partner of the project will be presented. To achieve this goal, analytical methods allowing the sampling, separation, identification and quantification of belowground volatile compounds have to be developed, taking into account their potential modifications in the rhizosphere once released by the roots. They enable the subsequent characterization and study of the interactions between barley and its rhizospheric partners chosen for this study. These interactions imply three types of organisms: beneficial organisms, pathogenic agents and plant and insect pests. Beneficial organisms can promote the growth of barley by the emission of volatiles; on the other hand barley can support their growth and metabolism. These phenomenons will be assessed by the study of 19 strains of plant growth-promoting bacteria (PGPR). Three pathogenic agents - two fungi (Fusarium culmorum and Cochliobolus sativus) and one virus (Barley yellow dwarf virus) - were chosen as they are known to cause various diseases on barley, especially on roots. The attractive or repellent effects of barley root volatiles on the pathogenic agents or their vectors, as well as the effect of volatiles on the diseases evolution will be evaluated. The project also includes several types of pests such as plants and insects. Plants can compete with barley for space and nutrients through volatile interactions. This will be assessed by the study of autotoxicity by barley itself and allelopathy with 8 weeds and a hemiparasitic plant (Rhinanthus minor). The effects of barley volatiles can also impact the severity of the attacks by insects. This part will be conducted with wireworms as they represent worldwide known pests, and aphids, through their viral vector role. Eventually, as soil characteristics can strongly influence the diffusion of volatile compounds, the diffusion behaviour of the identified volatile biomolecules through the soil will be modelled. Tritrophic interactions (e.g. insect-plant-pathogenic fungi) will be studied based on each bitrophic interaction results. Over-all, the Rhizovol project aims at improving the knowledge of interactions mediated by volatile compounds in the rhizosphere and at establishing new biocontrol methods that could contribute to integrated disease and pest management systems. [less ▲]

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See detailField evaluation of survival rates of Harmonia axyridis (Coleoptera: Coccinellidae) and other aphidophagous species
Vandereycken, Axel ULg; Durieux, Delphine ULg; Joie, Emilie ULg et al

Poster (2011, May 24)

The multicoloured Asian ladybird Harmonia axyridis Pallas (Coleoptera: Coccinellidae), previously introduced as a biological control agent against aphids, is now frequently considered as an intraguild ... [more ▼]

The multicoloured Asian ladybird Harmonia axyridis Pallas (Coleoptera: Coccinellidae), previously introduced as a biological control agent against aphids, is now frequently considered as an intraguild predator, consuming other aphids natural enemies. Interactions between this exotic ladybird and the other aphidophagous species present in Belgian agro-ecosystems such as Coccinella septempunctata, Episyrphus balteatus, Chrysoperla carnea are asymmetric to the benefit of H. axyridis. Many studies have already shown its predatory behaviour. This study focuses on the survival rate of three aphidophagous species Harmonia axyridis (Ha), Coccinella septempunctata (C7) and Episyrphus balteatus (Eb) in biological fields of potato and sugar beet. During June and July, hermetic cages were disposed on the crops with 10 larvae (second stage) of each aphidophagous. There were 4 combinations of insects placed inside the cages (1) Ha+Eb+C7+ aphids, (2) Ha+Eb+C7, (3) Eb+C7+ aphids and (4) Eb+C7. Each combination was repeated three times. Aphids (Aphis fabae) were added in excess when needed. In sugar beet fields, E. balteatus was the species with the highest mortality rate. The two coccinellid species had a better survival rate than E. balteatus. All aphidophagous species had higher mortality rates in absence of aphids. In potato, the survival rate of all aphidophagous was lower than sugar beet when aphids were present in excess probably because A. fabae reproduced less in potato. Nevertheless, we have observed in potato no mortality of C. 7-punctata during all its development cycle. This suggests that C. 7-punctata could use more efficiently aphids as food sources than H. axyridis. In potato and sugar beet, H. axyridis had the longest development cycle, being still at the larval stage when C. 7-punctata and E. balteatus reached the pupae stage. We can suppose that pupae of E. balteatus and C. 7-punctata were an alternative food (intraguild prey) to H. axyridis when aphids were lacking. Pupae are immobile and therefore more sensitive to the attack of predators. [less ▲]

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See detailStand structure of mosquitoes (Diptera: Culicidae) in farms and establishment of a new potential vector of West Nile Virus for Belgium
Boukraa, Slimane ULg; Zimmer, Jean-Yves ULg; Simonon, Grégory ULg et al

Poster (2011, May 24)

Many Diptera, spread across the globe are likely to play a role in the transmission of various human and animal diseases. Mosquitoes in particular, give rise to various countries on research increasingly ... [more ▼]

Many Diptera, spread across the globe are likely to play a role in the transmission of various human and animal diseases. Mosquitoes in particular, give rise to various countries on research increasingly expanded and deepened. To better understand the structure of the Culicidae at cattle farms in Belgium and identify habitats favorable to the development of each species, a taxonomic inventory was conducted in 2008 and 2009 in ten different sites. They fall into five natural regions: Condroz, Ardenne, Fagne-Famenne, Lorraine and Compine. The collection of mosquitoes is based on sampling of larvae in 13 biotopes such as water troughs, tires, abandoned utensils and temporary puddles or not. The results of the morphotaxonomic study of the mosquitoes collected in the different study sites show the presence of five species of Culicidae divided into three genera, Anopheles, Culex and Culiseta. Of the 1843 individuals examined in 2009, Culex pipiens represents 79.98% of the total harvest. A new species is reported for Belgium, Culex modestus Ficalbi, 1890. This species is one of the most important from an epidemiological and medical-veterinary entomology, given its role in the transmission of West Nile and myxomatosis viruses. [less ▲]

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See detailPredominant effects of host plant species on Aphis gossypii aphid and Episyrphus balteatus hoverfly in a tritrophic approach
Poligui, René Noël ULg; Francis, Frédéric ULg; Haubruge, Eric ULg

Poster (2011, May 24)

The aphid Aphis gossypii Glover (Homoptera: Aphididae) is known as worldwide important pest. It causes serious injuries to crops, but is also able to feed on various cultivated and wild plants. Some ... [more ▼]

The aphid Aphis gossypii Glover (Homoptera: Aphididae) is known as worldwide important pest. It causes serious injuries to crops, but is also able to feed on various cultivated and wild plants. Some programs are developped to control this aphid. In many studies , hoverfy Episyrphus balteatus De Geer (Diptera : Syrphidae) is used as an efficient and aphid specific predator in biological control systems. In this work, we study cotton aphid choice plant discrimination, the effect of host plants on its developpment(fecondity and mortality), and the E.balteatus foraging and oviposition behavior according aphid host plants. Biologic parameters were observed using various plant combinations. E.balteatus behavioral observations were conducted using the Noldus Observer v.5.0. A.gossypii makes discriminant choice of his hosts, with better preference on Cucurbita pepo L. (Cucurbitaceae) and Capsicum annuum L. (Solanaceae). Dacryodes edulis (G. Don) H.J. Lam. (Burseraceae) and Vicia faba L. (Fabaceae) are the midle choice while Hibiscus sabdariffa L. (Malvaceae) is less chosen. A.gossypii develops easily on C.pepo, and weakly on C.anuum, H.sabdariffa,and V.faba. D. edulis induces very high mortality to A.gossypii. According to E. balteatus behavior, A. gossypii is more attractive when infesting C .pepo and V.faba, less on H. sabdariffa. While non infested , the hoverfly more attractive plant is V.faba. Our results highlited the importance of interactions between aphids and their host plants and the prey-host influence on a specific aphid predatory hoverfy. Suitable ways in correlation with this work are to investigated to complete this study and perform aphids biological control. [less ▲]

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See detailScreening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens
Bafort, Françoise ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg et al

Poster (2011, May 24)

The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of ... [more ▼]

The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of hydrogen peroxide in hypoiodite (OI-) and hypothiocyanite (OSCN-) ions. Both ions have inhibitory action against pathogenic bacteria, fungi and viruses. Until now, the ions OI- and OSCN- applied separately from the LPS were not tested against plant pathogens. Seventeen KI/KSCN/H2O2 ratios with 3 dilutions for each one were tested against Botrytiscinerea. Results showed that the ions OI- and OSCN- gives a better % of inhibition with: -high concentration of KI+KSCN, -a ratio of KI/KSCN of 4.5 and -a ratioof (KI+KSCN)/H2O2 near 1. Increased dilution combined with lower KI+KSCN concentration reduced the efficacy of the ions. From the screening against B.cinerea, 4 ratios of KI+KSCN were selected and tested against 6 other phytopathogens. Results showed that the OI- and OSCN- ions give 87 and 100% inhibition against Penicillium expansum and Verticillium dahlia respectively. The inhibitory activity of the ions solution was ranged between 70 and 79% regarding Fusarium avenaceum, Phytophthora infestans and Colletotrichum lindemuthanium. The ions OI- and OSCN- showed an insufficient inhibitory activity against Septoria tritici. The screening method highlighted that high KI/KSCN ratios and low dilution were more efficient against phytopathogens. The efficient ratios will be further evaluated in vivo. [less ▲]

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See detailActivity-Flexibility and Stability Relationships as revealed by multiple mutants of a psychrophilic alpha-Amylase
Cipolla, Alexandre ULg; D'Amico, Salvino ULg; Feller, Georges ULg

Poster (2011, May 23)

Permanently cold environments, like polar regions, have been colonized by a great variety of psychrophilic organisms producing enzymes adapted to function efficiently at low temperatures. We have ... [more ▼]

Permanently cold environments, like polar regions, have been colonized by a great variety of psychrophilic organisms producing enzymes adapted to function efficiently at low temperatures. We have investigated the role of weak interactions in thermal adaptation of proteins by site-directed mutagenesis of the psychrophilc alpha-amylase (AHA) from the Antarctic bacterium Pseudoalteromonas haloplanktis. Two stabilized multiple-mutants (Mut5 and Mut5CC) have been constructed. The single mutations were selected by comparison of the presence of weak interactions in a mesophilic homolog from pig pancreas, PPA. The three enzymes AHA, Mut5 and Mut5CC have been analyzed by differential scanning calorimetry, thermal and chemical denaturation. The flexibility has been studied by acrylamide-induced fluorescence quenching. In order to investigate the kinetic origin of the gain in stability, the kinetics of unfolding and refolding in GdmCl have been monitored at 15°C. The newly introduced weak interactions stabilized the mutants, protected them against heat and chemical unfolding and also induced an effective loss of flexibility. In addition, the two multiple-mutants exhibit an increased optimum temperature for activity. The first results of kinetic studies show a similar refolding phase but differences between the three amylases in the unfolding phase. These results unambiguously support the capital role of weak interactions in the balance between activity, flexibility and stability and provide a better knowledge of the adaptation of enzymes to cold temperatures. [less ▲]

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See detailBelgo-Serbian explorations on the massif of Durmitor (Montenegro) since 2008
Peeters, Alexandre ULg

Poster (2011, May 21)

Abstract: This poster relates the results of the Belgo-Serbian explorations on the massif of Durmitor (Montenegro) since 2008. These explorations were only possible thanks to the Serbian cavers of ASAK ... [more ▼]

Abstract: This poster relates the results of the Belgo-Serbian explorations on the massif of Durmitor (Montenegro) since 2008. These explorations were only possible thanks to the Serbian cavers of ASAK that we met in Lazareva, training camp in Serbia. Thereafter, the Serbian cavers gave us the opportunity of an exploration on the massif of Durmitor. For the Belgian cavers, this exploration was part of the federal training project Explo2009, whose purpose is to train the young cavers underground exploration. Regarding the results of these explorations, this poster details the discovery of the three main caves: Pedina sa ledom pod Bandijerne, l’ébouleuse and Fliš. Pedina sa ledom pod Bandijerne has long been known because of its entry porch which can be seen from the camp. With the discovery of a new passage in the chamber that follows the entrance, the exploration of this new réseau was done by the Serbian cavers in 2008 and was continued by the Belgian cavers in 2009, reaching the depth of 141 m.L’ébouleuse, which is near the Minin Bogaz pass, has been discovered in 2009. It has been completely explored in several days, reaching the bottom of the cave at the depth of 113 m. Last but not least, the main result of these explorations is Fliš. The exploration of this deep cave began in 2009 and is still in progress. The depth of 432 m was reached in 2009 and then 582 m in 2010. This cave is characterized by deep pits in which we can see the sequence of the sedimentary rocks, called Flysch. [less ▲]

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See detailEvaluation of the use of VEGF111 for the treatment of tendon lesions.
Janssen, Lauriane ULg; Kaux, Jean-François ULg; Drion, Pierre ULg et al

Poster (2011, May 20)

Alterations of tendons are common pathologies resulting from repetitive or abnormal mechanical sollicitations. Very frequently lesions become chronic and may even lead to rupture. As there is no current ... [more ▼]

Alterations of tendons are common pathologies resulting from repetitive or abnormal mechanical sollicitations. Very frequently lesions become chronic and may even lead to rupture. As there is no current efficient treatment for curing this type of diseases, new therapeutic approaches are being tested and developed. Injection of platelet-rich plasma (PRP) seems to be a promising treatment by local release of growth factors. Among these factors, VEGF-A is known to induce positive effects on vascular functions and angiogenesis, and could be implicated in the healing process of tendons. Several isoforms of VEGF-A have been described in literature, including VEGF165 and 121. VEGF111 is encoded by exons 1-4 and 8a. The lack of exon 5 enables VEGF111 to resist to proteolytic degradation and the absence of exons 6 and 7 reduces its affinity for several macromolecules present on the cell surface and in the extracellular matrix. In vivo, it has been shown to be highly proangiogenic and diffusible. A 5mm defect was surgically performed in the Achilles tendon of 60 rats. Two hours after closure of the fascia and the skin, an injection within the wound was performed with PBS alone (n=30) or with PBS containing 100 ng of VEGF111 (n=30). 10 rats of each group were sacrificed at days 5, 15 and 30. The operated tendon was then carefully removed and collected for either immunohistochemical analyses or mechanical testing. At each time point, the section and the overall appearance of the repairing tendons were similar for PBS and VEGF111-injected tendon. As compared to controls, injection of VEGF111 seemed to promote a faster angiogenesis, although the number of samples was at this stage too low for performing reliable statistical analysis. Mechanical resistance to rupture of the repairing tendons was also measured. No difference between the two groups was observed after 5 or 15 days. By contrast, increased tensile strength was clearly evidenced in the VEGF-treated group after 30 days. These preliminary data seem to indicate a positive effect of a single VEGF111 injection for restoring the mechanical properties of tendons after their section. Additional experiments are planned for confirmation purposes and for further characterizing the model. It includes a “dose- response” analysis, the use of VEGF165 as an additional control and a study evaluating the effect of several injections. [less ▲]

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See detailZebrafish Skeleton Measurements using Image Analysis and Machine Learning Methods
Stern, Olivier ULg; Marée, Raphaël ULg; Aceto, Jessica ULg et al

Poster (2011, May 20)

The zebrafish is a model organism for biological studies on development and gene function. Our work aims at automating the detection of the cartilage skeleton and measuring several distances and angles to ... [more ▼]

The zebrafish is a model organism for biological studies on development and gene function. Our work aims at automating the detection of the cartilage skeleton and measuring several distances and angles to quantify its development following different experimental conditions. [less ▲]

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See detailLa guerre à Gaza (2009): état d'insécurité après la redéfinition des frontières du conflit israélo-arabe?
Wintgens, Sophie ULg; Piet, Grégory ULg; Stans, David ULg

Poster (2011, May 17)

Près de deux ans après la guerre à Gaza et l’opération israélienne « Plomb durci » (hiver 2008-2009), il est riche d’enseignements de tirer le bilan de cet événement ayant des répercussions locales ... [more ▼]

Près de deux ans après la guerre à Gaza et l’opération israélienne « Plomb durci » (hiver 2008-2009), il est riche d’enseignements de tirer le bilan de cet événement ayant des répercussions locales, régionales et internationales. La présente communication propose de donner les résultats d’une recherche menée pendant plus d’un an sur les conséquences de la guerre à Gaza (2009) à un niveau local (relation conflictuelle entre Israël, l’autorité palestinienne et le Hamas), et aux niveaux régional et international. La question de la frontière « virtuelle » entre Israël, ses voisins moyen-orientaux et ses partenaires internationaux est donc au centre de notre recherche. Cette communication se propose de livrer une vision originale de l’implication des relations internationales et de l’État dans la résolution d’un conflit, et de démontrer la rémanence d’un conflit dépassant les seules frontières de la Palestine et d’Israël. Pour ce faire, nous posons deux éléments essentiels dans la redéfinition des frontières du conflit : d’une part, l’opposition politique intra-palestinienne (autorité palestinienne vs. Hamas) et, d’autre part, les jeux de recomposition régionale autour du Hamas en tant que nouvel interlocuteur ou en tant qu’enjeu régional contre l'Etat israélien. Partant, trois axes de recherche sont privilégiés : le dépassement du conflit israélo-palestinien, la redéfinition des acteurs et enjeux régionaux et l’implication nouvelle d’acteurs étatiques hors de la région moyen-orientale. Le tout réside dans un état d’insécurité constant pour la région et pour l’État israélien au regard des enjeux et des recompositions régionales. La première approche nous permet de dépasser la seule lecture israélo-palestinienne du conflit en reprenant les stratégies israéliennes d’isolement du Hamas et les tensions entre l’autorité palestienne et l’acteur principal de la bande de Gaza. Ce que la guerre à Gaza (2009) a par exemple modifié dans le conflit global israélo-palestinien, c’est la prise en considération du Hamas en tant qu’interlocuteur dans les négociations. Avant cet événement, ce statut était exclu étant entendu que la scène internationale considérait le Hamas comme un groupe terroriste avec lequel la négociation ne pouvait se faire. La deuxième approche proposée par cette communication rejoint une analyse de la redéfinition des frontières du conflit israélo-palestinien en promouvant un élargissement de ce dernier : préférant le qualificatif « israélo-arabe ». Nous démontrerons en effet que la considération israélo-arabe du conflit a, à nouveau, un intérêt certain. Partant, notre analyse axe, d’une part, son argument sur une prise en compte du Hamas par les États régionaux. Cette distinction est nécessaire pour comprendre comment la cause palestinienne peut tantôt faire l’objet d’un réel soutien de la part d’État régionaux, comme la Turquie, mettant un terme à des contrats avec Israël dès le conflit, tantôt être un « prétexte » de la part d’États régionaux, utilisant et instrumentalisant la confrontation avec Israël pour servir leurs propres intérêts. Nous pourrons dans ce cadre recourir à une étude portant sur la manière dont l’opinion publique a perçu le rôle des acteurs régionaux avec la consécration du président iranien comme défenseur de la cause palestinienne et l'arrivée de la Turquie dans le jeu régional se détachant un peu plus de l’Union européenne. Enfin, il convient, dans une troisième approche, d’élargir notre étude à l’implication de la scène internationale dans la région et aux nouvelles alliances. Le cas français est intéressant à ce propos car il se trouve en tension entre un soutien à Israël, sa relation avec la Syrie et une volonté de revenir en tant qu’acteur important au Moyen-Orient. Au même titre, la position américaine et, plus particulièrement, la politique d’Obama souhaitant se démarquer de la politique de Bush (cf. discours du Caire, juin 2009) relèvent d’un intérêt certain pour notre étude des nouvelles frontières du conflit israélo-arabe. [less ▲]

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See detailInsertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Godaux, Damien ULg; Emonds-Alt, Barbara ULg; Cardol, Pierre ULg et al

Poster (2011, May 17)

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases ... [more ▼]

The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization. To discover new genes involved in hydrogenases activity, we are also planning to screen the same insertional library for mutants with attenuated levels of hydrogen photoproduction, using sensitive chemochromic sensor films which turn in blue in presence of hydrogen. We are currently making the chemochromic sensor WO3 films by dip-coating which is on the brink of being useable. [less ▲]

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See detailDétermination de la stabilité de l'huile de Cactus
Zine, Salma; El Hadek, Miloud; Hassani, Lalla Mina Idrissi et al

Poster (2011, May 12)

Les travaux que nous avons effectués sur l'étude de l'oxydation et de la conservation des huiles ont montré que l'huile d'argan peut être conservée pour une durée de 400 jours sous azote et à l'obscurité ... [more ▼]

Les travaux que nous avons effectués sur l'étude de l'oxydation et de la conservation des huiles ont montré que l'huile d'argan peut être conservée pour une durée de 400 jours sous azote et à l'obscurité. Les travaux que nous avons effectués sur l'étude de l'oxydation et de la conservation des huiles ont montré que l'huile d'argan peut être conservée pour une durée de 400 jours sous azote et à l'obscurité. [less ▲]

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See detailChitosan-based nanofibers for wound dressing
Aqil, Abdelhafid ULg; Tchemtchoua Tateu, Victor ULg; Colige, Alain ULg et al

Poster (2011, May 12)

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See detailProgress in cobalt-mediated radical polymerization
Debuigne, Antoine ULg; Hurtgen, Marie ULg; Piette, Yasmine ULg et al

Poster (2011, May 12)

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See detailSynthesis and characterization of reversibly core cross-linked micelles sensitive to reductive environment
Cajot, Sébastien ULg; Danhier, F.; Collodoro, M. et al

Poster (2011, May 12)

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See detailDesign of Photoreactive Orthosteric GABAA Ligands Potentially Useful for Studying Localization and Function of GABAA Receptors
Francotte, Pierre ULg; Nielsen, Birgitte; Krogsgaard Larsen, Povl et al

Poster (2011, May 08)

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See detailHigh-resolution measurements of an upwelling filament during the CAIBEX survey
Troupin, Charles ULg; Sangrà, Pablo; Arístegui, Javier et al

Poster (2011, May 02)

Upwelling filaments have been studied for several years. We aim to explain the general process in terms of potential vorticity: An injection of positive vorticity north of the Cape forces the jet to turn ... [more ▼]

Upwelling filaments have been studied for several years. We aim to explain the general process in terms of potential vorticity: An injection of positive vorticity north of the Cape forces the jet to turn offshore, in virtue of the principle of vorticity conservation. The Regional Ocean Modeling System (ROMS) is implemented in the Cape Ghir region, with horizontal resolution lower than 1 km. A set of process-oriented experiments has been designed in order to determine the mechanisms at the origin of the filament. [less ▲]

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See detailSpatial dynamics of wild boar population: Insights from a hunters' survey
Morelle, Kevin ULg; Lejeune, Philippe; Mengal, Coralie ULg

Poster (2011, May 02)

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See detailKynurenine administration suppresses cortical spreading depression in rat and the effect size is sex hormone dependent
Chauvel, Virginie ULg; Vamos, Eniko; Pardutz, Arpat et al

Poster (2011, May)

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See detailAssessing the World Ocean ventilation timescales with simple analogs - the leaky funnel model
Mouchet, Anne ULg; Deleersnijder, Eric

Poster (2011, May)

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See detailEffect of benzoic acid analogs on myeloperoxidase activity measured by a new technique to study their direct interaction with the enzyme.
Franck, Thierry ULg; Mazloum, Ali; Mouithys-Mickalad, Ange ULg et al

Poster (2011, May)

Myeloperoxidase (MPO) plays a key role in inflammatory response and constitutes a target for new drug development. The effects of some benzoic acid analogs were studied on the specific activity of human ... [more ▼]

Myeloperoxidase (MPO) plays a key role in inflammatory response and constitutes a target for new drug development. The effects of some benzoic acid analogs were studied on the specific activity of human MPO measured by SIEFED (“Specific Immunologic Extraction Followed by Enzymatic Detection”), an original method that consists of incubation of the compound with MPO, followed by capture of the enzyme by specific antibodies, washing (elimination of the compounds) and enzymatic detection of the immunocaptured enzyme. The compounds tested at 10-4, 10-5 and 10-6 M were studied in terms of structure activity relationship. Gallic acid (3,4,5-trihydroxybenzoic acid) with 3 hydroxyl groups had an important dose dependent inhibitory effect on MPO activity. Other molecules with less or without hydroxyl groups [3,4-dihydroxybenzoic acid, 2-hydroxybenzoic acid (salicylic acid) and benzoic acid] had rather an activator effect at 10-5 and 10-6 M. 2,4,6-Trihydroxybenzoic acid, with two hydroxyl groups adjacent to the carboxyl group, had a less efficient inhibitory effect. Caffeic acid (3,4-dihydroxycinnamic acid) with a propenoic acid group presented a dose dependent inhibitory effect on MPO activity contrary to its analog 3,4-dihydroxybenzoic acid. The esterification of the carboxyl group of gallic acid to obtain propyl gallate induced an activation of MPO at 10-5 and 10-6 M. Finally, the substitution of one or two hydroxyl groups by methoxyl ones (ferulic and syringic acids) decreased the efficiency of the molecules on the enzyme inhibition. The SIEFED technique appears as an innovative pharmacological tool to study the direct interaction of compounds with MPO. Number and position of hydroxyl groups and the extension of the carboxyl group of benzoic acid play a crucial role in the inhibition of MPO activity probably by facilitating the interaction with the active site or another elements of the enzyme structure. [less ▲]

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See detailTreatment of a case of trichurosis (Trichuris suis) in pigs
Caron, Yannick ULg; Delleur, Valery; De Bock, Bart et al

Poster (2011, May)

Trichuris suis infection was identified in a pig herd during an investigation into persistent diarrhea and weight loss in 10 recently introduced gilts. Eggs of the parasite were identified in faeces of ... [more ▼]

Trichuris suis infection was identified in a pig herd during an investigation into persistent diarrhea and weight loss in 10 recently introduced gilts. Eggs of the parasite were identified in faeces of gilts. Further investigations failed to detect other causes of diarrhea but, in all production stages, they allowed the detection of eggs of Trichuris suis that could explain pasty faeces sometimes observed in weaned sows and frequent diarrhea found in the early fattening period. The administration of levamisole (intramuscular injection) to gilts allowed the excretion of adult parasites, the treatment of diarrhea and the reduction of the number of eggs per gram of faeces. Taking into account the success obtained with levamisole in gilts, the equipment of the farm and the wish of the farmer no to inject finishing pigs, an unsuccessful treatment was tried based on levamisole in drinking water every 8 and then 5 weeks. Flubendazole was finally administrated in drinking water every 3 and then 5 weeks, reducing significantly the number of eggs per gram of faeces. [less ▲]

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See detailInvolvement of z-MMP-2 in Zebrafish lymphangiogenesis
Paupert, Jenny ULg; Pendeville, Hélène; Detry, Benoît ULg et al

Poster (2011, May)

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See detailOxygen, a tool for assessing ocean tracer transport models
Mouchet, Anne ULg; Campin, Jean-Michel

Poster (2011, May)

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See detailRadiation-induced malignant peripheral nerve sheat tumors – a report of 2 cases
PHAN BA, Remy ULg; BELACHEW, Shibeshih ULg; JEDIDI, Zayd ULg et al

Poster (2011, May)

We discuss the case of two patients who developped delayed malignant peripheral nerve sheath tumor (MPNST) following radiotherapy. Case report: the first patient is a sixty year-old woman with a 2 years ... [more ▼]

We discuss the case of two patients who developped delayed malignant peripheral nerve sheath tumor (MPNST) following radiotherapy. Case report: the first patient is a sixty year-old woman with a 2 years history of right cervicobrachial pain and weakness. The neurological examination depicted severe weakness, atrophy and loss of sensation in the right C5 and C6 territories. A subclavicular hardened and enlarged lymph node was noted. Her past medical history was notable for a Hodgkin’s disease (HD) treated with radiation therapy (>40Gy) 35 years earlier. Brachial plexus MRI revealed a tumoral mass arising from the right brachial plexus. Biopsy of the subclavicular mass revealed a poorly differentiated malignant tumour consisting of spindle cells showing moderate polymorphism and a high mitotic index. Immunohistochemistry showed positivity for the S-100 protein, the CD56 and for the epithelial membrane antigen (EMA) and a diagnosis of MPNST of the brachial plexus was proposed. The second case is a 36 year-old man referred for a history of right sciatic neuralgia that appeared 3 years earlier. The medical history of the patient was notable for a right seminoma, treated by orchidectomy and prophylactic radiotherapy (24 Gy) 5 years earlier. The neurologic examination revealed right L5 and S1 radicular territories involvement, and the CT of the pelvis demonstrated a nodular mass at the level of the greater sciatic foramen. A surgical biopsy was performed and the neuropathological findings were consistent with a diagnosis of low-grade MPSNT. Discussion: MPNSTs are rare tumors accounting for 3 to 10% of all tissues sarcomas. Half of the cases described are sporadic, while the other half tend to appear in patients suffering from tumor prone conditions, such as neurofibromatosis type 1. Although secondary neoplasms are known complications of radiotherapy, descriptions of peripheral nerve sheath tumors (PNST) are scarce. The exact pathophysiology of radiation-induced PNSTs remains unclear but vascular alterations, direct damages to axon or Schwann cell and nerve compression by soft tissue fibrosis are thought to play a role. Although surgical removal sometimes followed by chemotherapy is the mainstay of MPNSTs, they usually carry a poor prognosis. Our 2 cases emphasize that the possibility of radiation-induced MPNST has to be kept in mind when investigating a localized neuropathy in a previously irradiated area. [less ▲]

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See detailAcaricidal activities of Santolina Africana and Hertia Cheirifolia, two endemic plants of north Africa, on the two spotted spider mite (Tetranychus urticae)
Attia, Sabrine; Grissa, Kaouthar Lebdi; Le Goff, Guillaume et al

Poster (2011, May)

The two-spotted spider mite, Tetranychus urticae Koch, is a worldwide pest which feeds on a large variety of plant families. Because its resistance to acaricides is spreading rapidly, the development of ... [more ▼]

The two-spotted spider mite, Tetranychus urticae Koch, is a worldwide pest which feeds on a large variety of plant families. Because its resistance to acaricides is spreading rapidly, the development of new biological control tactics for population management is crucial. Plant extracts, such as Santolina africana and Hertia cheirifolia (Asteraceae), may represent viable alternatives, since they are currently considered to be minimumrisk pesticides. Although these two plants are known for their biocidal properties, the extract concentration that provides the most efficient control has not yet been precisely determined. In this study, we conducted a series of laboratory experiments to determine the susceptibility of adult females to different concentrations of S. africana and H. cheirifolia extract. These plants were steam-distilled and sprayed using a Potter spray tower. Mortality and fecundity were measured upon treatment with extract concentrations ranging from 0.07 to 6.75 mg/L. Female mortality increased with concentration, with LD50 values of 2.35 mg/L for S. africana and 3.43 mg/L for H. cheirifolia, respectively Reduced fecundity was previously observed at concentrations of 0.07, 0.09 and 0.29 mg/L .The chemical composition of the two oils were characterised by GC-MS. The most abundant components of the S. africana oil were terpinen-4-ol (44%), α- terpineol (11.31%) and borneol (6.73%). Camphor (15.11%), terpinen-4-ol (14.2%), α- terpineol (9.3%) were characteristic of the Hertia oil. [less ▲]

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