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See detailStructural and morphological Control of TiO2 nanoparticles by selective adsorption of organic molecules
Dufour, Fabien ULg; Durupthy, Olivier; Cassaignon, Sophie et al

Poster (2011, September)

Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde ... [more ▼]

Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde (TiO2), is one of the most interesting material in many applications, such as photocatalysis, photovoltaics, photo/electrochromics or sensors. [1] In addition to an influence of the nanoparticles size, recent works on the anatase phase demonstrated the effect of the expressed surfaces nature on the nanoparticles photoreactivity. [2] Sol-gel method has been used to obtain a wide range of anatase nanoparticles sizes and morphologies by the control of concentrations, ions in solution, solution acidity and aging parameters. [3] In order to avoid the brookite phase often obtained with the anatase phase, microwave heating was efficiently used. Furthermore, conventional heating method has been compared with hydrothermal and microwave activated synthesis. The precise morphological control has been achieved by selective adsorption of organic molecules during synthesis: different shapes of anatase nanoparticles have been synthesized. [4,5]. The influences of synthesis parameters and the organic molecules nature on the nanoparticles structure and morphology have been analysed with various techniques, such as XRD and HRTEM. [less ▲]

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See detailPili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project)
Imperi, Monica; Rinaudo, Daniela; Creti, Roberta et al

Poster (2011, September)

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI ... [more ▼]

Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI (Design of a Vaccine Against Neonatal Infection) project. Methods GBS isolates from pregnant women (PW) and cases of newborn infection (NI) were collected in 8 European countries (Belgium, Bulgaria, Czech Republic, Denmark, Germany, Italy, Spain, United Kingdom) during 2009/10 under the auspices of DEVANI. Total no. of strains examined was 1078 and 192 from PW and NI, respectively. Isolates were screened by multiplex PCR and FACS analysis to evaluate respectively gene presence and surface-exposure of pili. Results The most common gene patterns found were PI-2a alone, PI 1+2a and PI 1+2b, while the PI-2b gene alone was very rare. The most prominent result was that a majority of isolates from NI carried the PI-1+2b gene pattern, while the most common pattern among PW was PI-1+2a. Most of analyzed strains express at least one pilus on their surface. Conclusions All isolates contained at least one gene coding for pili. When present pili 2a and 2b were highly surface exposed. [less ▲]

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See detailTissue Proteomics of Epithelial Ovarian Cancers.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, September)

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See detailMALDI Mass Spectrometry Imaging for the Screening of Epithelial Ovarian Cancer Biomarkers.
Boyon, Charlotte; Longuespée, Rémi ULg; Kerdraon, Olivier et al

Poster (2011, September)

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See detailSedimentology of a continuous Givetian-Frasnian carbonate succession in Sauerland (Germany) and MS comparison with the time-equivalent ones of Ardennes (Belgium) and Moravia (Czech Republic)
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Boulvain, Frédéric ULg et al

Poster (2011, September)

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick ... [more ▼]

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick) covers a well constraint stratigraphic interval starting at the base of the Givetian (Stritzke 1991;Aboussalam et al., 2003) and according to our new datations ending within the Lower Famennian. The Middle-Upper Devonian shelf-edge within the Rheinisches Schiefergebirge can be traced from the supposed position along the southern rim of the Dinant Syncline and the Eifel Synclines, northwards along a line connecting the southern margin of the Devonian reefal outcrops of Attendorn and Brilon (Krebs 1967, 1974). The depositional setting of the investigated section corresponds to complex slope and basinal environments where reworked material from the proximal Brilon platform (located to the north) and basin deposits coexist. Thus, this section allows to follow the evolution of the Givetian-Frasnian Brilon (e.g., Machel 1990; Stritze 1990, 1991) platform in a deeper setting. Petrographic analysis of more than 300 thin-sections leads to the identification of 9 microfacies which are integrated into a palaeoenvironmental model. Microfacies curve evolution shows two main trends. A shallowing upward trend ending within a typical proximal slope setting (dismantling of the platform) followed by a deepening upward trend which is characterized by several meter of pelagic mudstone within the upper part of the studied section. Magnetic susceptibility variations in sedimentary rocks, have commonly been interpreted as related to variations of detritic inputs through climatic or sea level changes (Crick et al., 1994). The magnetic susceptibility (MS) study of more than 330 samples from this long-time fore-reef carbonated succession is an opportunity to better constrain our sedimentological interpretations. To do so, we propose a comparison between general MS trends and some parameters such as microfacies and relative sea level fluctuations interpreted on the basis of the sedimentological study. The relatively long stratigraphic interval covered by the Burgberg section offers a good opportunity to compare our data with the time equivalent Devonian sections of the Ardennes (Belgium) and Moravian karst area (Czech Republic) (Boulvain et al., 2010). And thus to test the magnetic susceptibility tool for long distance correlation between stratigraphically well constraint sections. [less ▲]

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See detailAssessing the impacts of technical uncertainty on coupled surface/subsurface flow model predictions using a complex synthetic case
Wildemeersch, Samuel ULg; Goderniaux, Pascal ULg; Orban, Philippe ULg et al

Poster (2011, September)

According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful ... [more ▼]

According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful management tools in this context since they can predict system responses to future stresses. However, numerical modelling at such a scale faces specific issues linked to (1) the representation of the geological and hydrogeological complexity, (2) the uneven level of characterisation knowledge, (3) the representativity of measured parameters and variables in the field, and (4) the CPU time needed for solving the numerical problem. Assumptions and simplifications made for dealing with these issues can lead to a series of models differing by their complexity and by the reliability of their predictions. Consequently, modellers have to find a compromise between complexity and reliability. The main objective of this research is to estimate the impacts of technical uncertainty, which is the uncertainty related to the numerical implementation, on groundwater flow model predictions. To reach that objective, the methodology consists in comparing reference predictions (hydraulic heads and flow rates) of a complex and close to reality synthetic case with the predictions provided by a series of simplified models (coarse spatial discretisation, coarse time discretisation, simplified law in the unsaturated zone). The synthetic case reflects the main characteristics found in groundwater bodies of South Belgium (Condroz region of Wallonia), characterised by a succession of limestone synclines and sandstone anticlines. The numerical model is developed with the fully-integrated surface/subsurface flow and transport code HydroGeoSphere using a mesh refined along the surface water network (153027 nodes and 269872 elements). A 5-year reference transient simulation, with daily stress factors is performed. The simulated hydraulic heads and flow rates constitute the reference observations and predictions for the comparison with the simplified models. The simplified models tested differ by their horizontal (500 m vs. 1000 m element size) and vertical (8 layers vs. 3 layers) spatial discretisations, their time discretisation (daily vs. monthly stress factors), and the type of constitutive law used for simulating the unsaturated flow (linear vs. van Genuchten). The models are run with the same parameter values than those used in the reference model to evaluate the deterioration in model predictions due to technical uncertainty. Additionally, some of the models are calibrated with the inverse modelling code PEST to distinguish how far a model calibration can possibly compensate for technical uncertainty. Then, predictions from each simplified model are compared with the reference predictions of the synthetic case. Then, the simplified models are ranked using several model performance criteria. Results of this research provide guidelines for the numerical implementation of groundwater flow models at the water body scale with respect to specific groundwater management objectives. [less ▲]

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See detailImpurity fingerprints for the identification of counterfeit medicines - a feasibility study
Sacré, Pierre-Yves ULg; Deconinck, Eric; Daszykowski, Michal et al

Poster (2011, September)

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See detailMolecular histology for epithelial ovarian cancers biomarker hunting: new issues for biology and pharmacology.
Longuespée, Rémi ULg; Boyon, Charlotte; Kerdraon, Olivier et al

Poster (2011, September)

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See detailEnzymatic Interesterificationof Palm oil and Fractions: A Calorimetric Study
Danthine, Sabine ULg; De Clercq, Nathalie; Lefebure, Emilie ULg et al

Poster (2011, September)

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See detailTime and space matter when monitoring faecal pollution : searching for efficient sampling designs in relation to hydrological regimes.
Cauchie, Henry-Michel ULg; Burnet, Jean-Baptiste; Ogorzaly, Leslie et al

Poster (2011, September)

Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event ... [more ▼]

Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event. Therefore, classical monitoring schemes based on low, fixed sampling frequency turned out to inefficiently estimate the microbial water quality. Several studies have therefore been carried out on important drinking water resources (springs and surface reservoir) in order to understand the link between the faecal contamination events and the hydrology and the hydrology of these waterbodies. Physico-chemical and hydrological variables that could serve as relevant indicators for triggering selective sampling were also investigated. [less ▲]

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See detailEnzymatic interesterification of palm oil and fractions: monitoring the degree of interesterification using different methods.
De Clercq, Nathalie; Danthine, Sabine ULg; Nguyen, Mai et al

Poster (2011, September)

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See detailGroßer Lebensmittel-assoziierter Ausbruch durch rekombinante Noroviren GII.g/GII.1 in einem süddeutschen Krankenhaus
Hoffman, Dieter; Mauroy, Axel ULg; Protzer, Ulrike et al

Poster (2011, September)

a) Einleitung Noroviren sind derzeit die häufigsten Gastroenteritiserreger. Durch ihre niedriger infektiöse Dosis und Umweltstabilität verursachen sie häufig Ausbrüche in Krankenhäusern und anderen ... [more ▼]

a) Einleitung Noroviren sind derzeit die häufigsten Gastroenteritiserreger. Durch ihre niedriger infektiöse Dosis und Umweltstabilität verursachen sie häufig Ausbrüche in Krankenhäusern und anderen Gemeinschaftseinrichtungen. Wir berichten über den größten Norovirusausbruch in einem Münchner Krankenhaus der vergangenen Jahre mit über hundert infizierten Patienten und insgesamt 28 betroffenen Mitarbeitern aus verschiedenen Bereichen. b) Material/Methoden Wir wiesen Noroviren GII durch real time Amplifikation eines 94 bp am 3´Ende des Nichtstruktur Polyprotein nach. Sieben positive Proben, aus verschiedenen Bereichen des Klinikums einschließlich zweier Außenstellen, sowie Proben von 2 Küchenmitarbeitern wurden sequenziert. Dabei untersuchten wir 1110 bp, bestehend aus Polymerase- und Capsidsequenzen. Das Ausbruchsmanagement bestand aus einer task force, die neben dem ärztlichen Direktor aus Ärzten, Pflegekräften, Hygieniker und Virologen Apotheke, Einkauf, und Küchenbereich bestand. Zu den Maßnahmen gehörten unter anderem Abfrage aller betroffenen Stationen mit mindestens täglicher Aktualisierung der line-list, Umstellen desinfizierender Maßnahmen, sehr eng kontrolliertes Patientenmanagement (Verlegungen nur nach Rücksprache mit der Hygiene), Begehung aller betroffenen Stationen und der Küche, Abfrage der Nahrungsmittelaufnahme durch Fragebögen, Fortbildung des Personals und enge Kooperation mit den zuständigen Behörden. c) Ergebnisse Am 15.06. traten bei Patienten erstmalig Übelkeit und Erbrechen auf, am Folgetag wurde bei den ersten Patienten Noroviren in Stuhlproben nachgewiesen. Die letzte Neuinfektion wurde am 23.06. registriert, so dass der Ausbruch bereits am 04.07. in Absprache mit dem RGU München für beendet erklärt wurde. Insgesamt wurde Norovirus RNA bei 116 Patienten auf 34 Stationen sowie 28 Mitarbeitern nachgewiesen. Zweiundzwanzig Mitarbeiter waren in der direkten Patientenversorgung tätig, eine Mitarbeiterin arbeitete im Laborbereich. Fünf der Mitarbeiter waren im Küchenbereich beschäftigt und sind damit die wahrscheinlichste Infektionsquelle. Sie gaben keine bzw. nur wenig ausgeprägte Symptome an, mit denen sie weiterarbeiteten. In den bisher untersuchten Lebensmittelrückstellproben konnten keine Noroviren detektiert werden. Alle untersuchten Sequenzen entsprachen einem rekombinanten GII.g/GII.I Stamm. Die ähnlichsten publizierten Sequenzen unterscheiden sich nur durch 17 Nukleinsäure-, und 3 Aminosäureaustausche d) Schlussfolgerungen Wir beschreiben hier den größten Norovirenausbruch in Münchener Kliniken der letzten Jahre. Er breitete sich rasch aus und trat außerhalb der üblichen Norovirussaison auf. Durch schnelle Einleitung strikter Hygienemaßnahmen und einer engen Überwachung der betroffenen Stationen fanden wir nach 10 Tagen keine Neuinfektionen und konnten den Ausbruch zeitnah für beendet erkären. Erstmalig wiesen wir den rekombinanten Stamm GII.g/II.1 in unserem Universitätsklinikum nach. Fehlende oder niedrige Immunität der exponierten Personen könnte die initial rasche Ausbreitung begünstig haben. Zusammenfassend zeigen unsere Daten, dass bei Gastroenteritisfällen auch in den Sommermonaten mit Noroviren gerechnet werden muss. Diese können auch von oligo- oder asymptomatischem Personal ausgehen. Schnelle Erregerdiagnose und entsprechende Hygienemaßnahmen sind wesentlich in der Bekämpfung der Infektion. [less ▲]

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See detailEffect of Deferoxamine Mesylate on Freezability of Blood Supplemented Canine Semen
Van Den Berghe, Femke ULg; Rijsselaere, Tom; Van Soom, Ann et al

Poster (2011, September)

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See detailTetanus following castration in a buck
Lamain, Guillaume ULg; Guyot, Hugues ULg

Poster (2011, September)

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See detailSuccessful reduction of cholelithiasis in a Holstein cow
Lamain, Guillaume ULg; Frisee, Vincent ULg; Ramery, Eve ULg et al

Poster (2011, September)

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See detailTail amputation in a Belgian blue bull
Lamain, Guillaume ULg; Sartelet, Arnaud ULg; Touati, Kamal ULg

Poster (2011, September)

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See detailEstimation du débit de filtration glomérulaire en transplantation rénale: étude multicentrique d'évaluation de la performance de la cystatine C
Masson, I.; Maillard, N.; Jaafar, A. et al

Poster (2011, September)

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See detailA new longirostrine ichthyosaur (Reptilia) from the Toarcian of France broadens the ecological diversity of the genus Temnodontosaurus
Martin, Jeremy; Fischer, Valentin ULg; Vincent, Peggy et al

Poster (2011, September)

The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids ... [more ▼]

The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids were occupying the lower trophic levels. We describe here an almost complete skeleton of this successful genus from the middle Toarcian (Lower Jurassic) of the Beaujolais foothills near Lyon, France, and assign it to a new species of Temnodontosaurus. This specimen exhibits cranial peculiarities such as a thin, elongated, and likely edentulous rostrum, as well as a reduced quadrate. Such morphological combination indicates dietary preferences that markedly differ from other species referred to as Temnodontosaurus. Despite a conservative postcranial skeleton, we propose that Temnodontosaurus is one of the most ecologically diverse genera of ichthyosaurs, including apex predators, small and soft prey longirostrine hunters, and generalized forms. Ammonites collected along the described specimen indicate that the new species is younger (bifrons ammonite zone) than most known Toarcian ichthyosaurs and therefore slightly postdates the severe environmental changes and marine invertebrate extinctions that occur during the Toarcian Oceanic Anoxic Event. The present study hence raises the question whether the speciation of Temnodontosaurus towards a new ecological niche, may have been a consequence of the post-crisis marine ecosystem reorganization. [less ▲]

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See detailDevelopment of a quantitative risk assessment for cheese made from raw goat milk contaminated by Listeria monocytogenes
Delhalle, Laurent ULg; Ellouze, Mariem; Clinquart, Antoine ULg et al

Poster (2011, September)

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal ... [more ▼]

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal Agency for the Safety of the Food Chain in 2005. The source of the contamination was related to a shedder goat, excreting 2.6 log cfu (colonies forming units) L. monocytogenes / ml without any clinical symptom. On the basis of the collected data, a quantitative microbial risk assessment model was developed covering the production chain from the milking of goats until the consumed products. Predictive microbiology models were used to simulate the growth of L. monocytogenes during the process of cheeses made from goat raw milk. The modular exposure assessment model showed a significant growth of L. monocytogenes during chilling and storage of the milk collected the day before the cheese production (increase of 1.7 log cfu/ml for the median) and during the step of starter and rennet adjunction to milk (increase of 0.8 log cfu/ml for the median). The median estimated final result (in the fresh cheese) was equal to 3.5 log cfu/g. The model estimates (expressed as median final result issued from the exposure assessment) were realistic compared to the number of L. monocytogenes measured in the fresh cheese (3.6 log cfu/g) reported during the cheese contamination period. The average number of expected cases of human listeriosis was between 0 and 1 for a high-risk sub-population and 0 for a low-risk healthy sub-population. Scenario analysis was finally performed to identify the most significant factors and aid in developing priorities for risk mitigation. Thus, by using quantitative risk assessment and predictive microbiology models, this study provided valuable information to identify and to control critical steps in a local production chain of goat cheese made from raw milk. [less ▲]

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See detailSeagrass response to in situ trace element contaminations
Richir, Jonathan ULg; Luy, Nicolas; Lepoint, Gilles ULg et al

Poster (2011, September)

The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of ... [more ▼]

The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of knowledge regarding to that seagrass contamination and decontamination kinetics. For the first time, Posidonia were experimentally in situ contaminated in 2009, at 10m depth in Calvi Bay (Corsica), by a mix of 15 TE (Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Ag, Cd, Pb and Bi). Trace element concentrations were measured by DRC-ICP-MS in leaves, rhizomes and in overall shoots sampled at regular time intervals. P. oceanica immediately accumulated pollutants from the beginning of experiments; once contaminations ended, TE concentrations came back close to their original levels within 2 weeks, or at least showed a clear decrease. Posidonia leaves exhibited different uptake kinetics for many elements (Cr, Cu, Zn, Ag, Cd, Pb and Bi): the younger growing leaves incorporated chemicals more rapidly than the older ones. Rhizomes did not show any clear trend, except for Cu, Zn and Bi. These results demonstrate that Posidonia is a very sensitive sentinel to immediately delineate punctual pollutions similar to what might be measured in contaminated Mediterranean waters. The very good response of Posidonia leaves to pollutant short term expositions suggests their routine use in regularly scheduled monitoring programs. Nevertheless, to by-pass Posidonia leaves deciduous character and their capability to detoxify rapidly, long term accumulation recordings also necessitate below-ground tissues analyses. [less ▲]

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See detailKinetics and diffusional limitations in nanostructured heterogeneous catalyst with controlled pore texture
Pirard, Sophie ULg; Hermans, Sophie; Devillers, Michel et al

Poster (2011, September)

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See detailPerinatal depression in adolescent mothers : a public health concern
Noirhomme-Renard, Florence ULg; Tancredi, Annalisa; Gosset, Christiane ULg

Poster (2011, September)

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See detailEffeti comportamentali dell'endosulfan su girini di Rana dalmatina
Lavorato, Manuela; Bernabé, I.; Tripepi, S. et al

Poster (2011, September)

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See detailAnt-aphid mutualism - A question of microorganisms?
Fischer, Christophe ULg; Patris, Geoffrey; Duriaux, Adrien ULg et al

Poster (2011, September)

It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection ... [more ▼]

It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of view, this phenomenon has been well studied, but its chemistry and semiochemical mechanisms are still largely unknown. This study aims to identify semiochemicals involved in the establishment of this relation and their sources, using both chemical and behavioral methods. Bioassays revealed that the greatest part of ant attraction toward aphid colonies is due to honeydew volatile compounds; enabling ant scouts to find more quickly aphid colonies and distantly recognize myrmecophilous species. Many of those VOCs seeming to have microbial origins, the main honeydew microorganisms have been isolated and their roles in VOCs production and ant attraction have been investigated. [less ▲]

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See detailJUDE: A Far-UV Imager for JUICE
Grodent, Denis ULg; Bunce, Emma; Bannister, Nigel et al

Poster (2011, August 31)

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See detailWhen MIR spectrometry helps to promote a local and vulnerable breed
Colinet, Frédéric ULg; Dehareng, Frédéric; Dardenne, Pierre et al

Poster (2011, August 31)

The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of ... [more ▼]

The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of calving). Due to its dual purpose type, income generated by both milk and meat is more stable and more flexible in responding to market fluctuations. Registered DP-BB cows are milk recorded (one of the conditions for them to be registered as DP-BB and therefore to get AEM subsidies). Since near 4 years, during routine milk recording, nearly all mid-infrared (MIR) spectra generated at the milk labs and the information of test-day records were collected in a database. Calibration equations using the MIR spectrometry were developed permitting the prediction of several bovine milk components (e.g., fatty acids (FA)). Their application on the MIR spectral database would allow comparing milk composition from 920 DP-BB and 52,497 Holstein cows (selected cows had a proportion of Holstein or DP-BB in their breed composition of at least 90%). On average, each cows had 6 test-day records with MIR spectra in the database. MIR predictions were analyzed using GLM procedure with 5 fixed effects (breed, herd, lactation number, month of test-day recording and lactation stage); values presented are lsmeans (± s.e.) of the breed. Although milk and fat yields were lower for DP-BB, their FA proportions in fat were different from Holstein. Indeed, there are 66.0% (0.2) and 67.6% (0.0) of saturated FA in fat of milk for DP-BB and Holstein, respectively. Furthermore, the DP-BB milk fat was richer in omega-9 (20.7% (0.2) vs. 19.6% (0.0) for DP-BB and Holstein, respectively). Use of MIR predictions may help stakeholders to promote milk and future dairy products from DP-BB. [less ▲]

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See detailIntegration of foreign breeding values for stallions into the Belgian genetic evaluation for jumping horses
Vandenplas, Jérémie ULg; Janssens, Steven; Gengler, Nicolas ULg

Poster (2011, August 30)

The aim of this study was to test the integration of foreign estimated breeding values (EBV) for stallions into the Belgian genetic evaluation for jumping horses. Belgian breeders import horses from ... [more ▼]

The aim of this study was to test the integration of foreign estimated breeding values (EBV) for stallions into the Belgian genetic evaluation for jumping horses. Belgian breeders import horses from neighbouring countries for which foreign information is needed as prior to estimate a more accurate EBV. The Belgian model is a bivariate repeatability BLUP animal model. For the year 2009, pedigree and data files contained 101 382 horses and 712 212 performances. 98 French and 67 Dutch stallions were selected and their foreign EBVs were converted into Belgian national trait. Associated reliabilities were also estimated. A Bayesian approach (Legarra et al., 2007) was applied to integrate this prior information into the Belgian evaluation. It led to a slight modification of the average EBV and of the standard deviation for the whole population. It also led to a new Belgian ranking of the foreign stallions more similar to foreign rankings. However, the adequacy of the Belgian model was not damaged. With regards to prediction ability, Bayesian evaluations using conversion equation estimated by Weighted Least Squares procedure predicted the best traditional EBVs of the year 2009 for the French stallions. For the Dutch ones, it were the evaluations associated to the conversion equation based on Wilmink et al. (1986). For both countries, Bayesian evaluations using conversion equation based on Goddard (1985) improved the most the stability of EBVs. Finally, integration of French and Dutch information improved reliabilities of the Bayesian EBVs of at least 5% and 2% for French and Dutch stallions, respectively. These results confirm the interest to integrate foreign information into the Belgian evaluation for jumping horses. [less ▲]

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See detailGender's best friend effect on depression in adolescence
Boulard, Aurore ULg; Born, Michel ULg

Poster (2011, August 26)

The development of intimacy during the adolescence is considered by several authors as a central developmental task (Sharabany et al., 2008, Erber & Erber, 2001) and can be seen as a predictor of any ... [more ▼]

The development of intimacy during the adolescence is considered by several authors as a central developmental task (Sharabany et al., 2008, Erber & Erber, 2001) and can be seen as a predictor of any psychosocial adjustment. The purpose of this study is to examine the relationship between peer attachment, parent attachment and depressive mood in adolescence. [less ▲]

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See detailPotential effects of blood contaminants on immune responses in harbour seals (Phoca vitulina)
Dupont, Aurélie ULg; Weijs, Liesbeth; Siebert, Ursula et al

Poster (2011, August 23)

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See detailDetection of aggregative behaviour in binary choice experiments
Brostaux, Yves ULg

Poster (2011, August 22)

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments ... [more ▼]

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments. Analysis of these experiments reveals specific characteristics (unequal counts between repetitions, dependence of individual choices) that make the traditional adjustment tests fail. We demonstrate that the use of generalized linear models can circumvent these pitfalls and deliver a reliable diagnosis on the social behaviour of the studied invertebrates. The strength of this behaviour can then be evaluated through the use of common correlation models. [less ▲]

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See detailUse of Chrysoperla carnea larvae for biological control of immature stages of Leptinotarsa decemlineata
Sablon, Ludovic ULg; Lejeune, Renaud ULg; Haubruge, Eric ULg et al

Poster (2011, August 13)

The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is an important economic pest of potato which populations are effectively controlled by insecticides. However, development of resistance ... [more ▼]

The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is an important economic pest of potato which populations are effectively controlled by insecticides. However, development of resistance along with the poor image of unnaturally management methods pushes researchers to develop alternative "greener" methods. Chrysoperla carnea is a generalist predator whose larvae are very voracious on any types of soft-bodied insects. In laboratory assays, we demonstrated a good predatory potential of lacewing larvae against eggs, first and second CPB larval instars. The third lacewing larval instar showed an important consumption of CPB immature stages. Indeed, when looking at the daily consumption, we found that prey consumption by the third larval instar was 3-fold higher compared to the two first instars. Partial or total consumption of prey was also numbered. Different proportions of partial/total consumption were found and these depend on the lacewing larval stage. This study provides new perspectives for possible use of C. carnea as a biological agent to control CPB. Nevertheless, additional work has to be conducted under semi-natural and field to completely evaluate the lacewing potential as a CPB predator. [less ▲]

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See detailInfluence of temperature on conservability of chilled vacuum packed beef from different origins
Didimo Imazaki, Pedro Henrique ULg; Maréchal, Aline; Nezer, Carine ULg et al

Poster (2011, August 07)

The objective of this experiment was to study the conservability of chilled vacuum-packed meat depending on storage temperature (–1 °C vs. +4 °C) during the last third of their shelf life. Physicochemical ... [more ▼]

The objective of this experiment was to study the conservability of chilled vacuum-packed meat depending on storage temperature (–1 °C vs. +4 °C) during the last third of their shelf life. Physicochemical parameters (pH and colour) and microbiological growth (total aerobic bacteria, lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp. and Brochothrix thermosphacta) of Longissimus dorsi samples from different origins (United Kingdom and Ireland, Australia and Brazil) were measured at: i) 2/3 of their shelf life and ii) the end of their shelf life. Sample bacteria population growing on MRS was identified by API 50 CHL strips. Unlike Irish and British samples, pH of some Australian and Brazilian samples decreased during conservation. The colour of the samples remained stable and it did not seem to be influenced by temperature. All samples conserved at –1 °C presented a satisfactory microbiological quality at the end of their shelf life (British and Irish meat = 35~45 days; Australian meat = 140 days and Brazilian meat = 120 days). On Australian and Brazilian samples, temperature did not influence total aerobic bacteria growth, but conservation at +4 °C favoured lactic acid bacteria and Enterobacteriaceae growth. API 50 CHL strip identifications revealed the presence of bacteria like Lactobacillus brevis, Carnobacterium maltaromaticum and Lactobacillus fermentum, which occur naturally in fresh meat and are known for their bioprotective effect against other microorganisms. Further analyses are being carried out using molecular methods in order to study the initial bacteria population diversity and it evolution during storage. [less ▲]

Detailed reference viewed: 227 (16 ULg)
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See detailNew method for establishing a network of operational warning of Septoria leaf blotch disease in winter wheat
El Jarroudi, Moussa ULg; Giraud, Frédéric; Defosse, Philippe et al

Poster (2011, August 06)

Detailed reference viewed: 16 (0 ULg)
Peer Reviewed
See detailRegional-based typology of the main fungal diseases affecting winter wheat.
El Jarroudi, Moussa ULg; Giraud, Frédéric; Delfosse, Philippe et al

Poster (2011, August 06)

Detailed reference viewed: 15 (0 ULg)
Peer Reviewed
See detailGenome-wide epistasis screening for asthma associated traits
Gusareva, Elena ULg; Huyghe, Jeroen; Van Steen, Kristel ULg

Poster (2011, August 01)

Genome-wide association (GWA) studies of asthma and associated traits have identified numerous genes. A substantial portion of the heritability of these traits remains unexplained. Some variants, not ... [more ▼]

Genome-wide association (GWA) studies of asthma and associated traits have identified numerous genes. A substantial portion of the heritability of these traits remains unexplained. Some variants, not detectable via main effects GWA study may manifest themselves only in interaction with other variants. To search for interacting genes involved in regulation of asthma associated traits (total IgE, eosinophils, FEV1, FVC, FEV1/FVC) we performed GWA epistasis screening in two family groups of asthma patients:CAMP (Childhood Asthma Management Program:814 cases and 467 trios) and CARE (Childhood Asthma Research and Education:796 cases and 338 trios) [dbGaP accession number phs000166.v1.p1.c1]. Individuals were genotyped with the Aymetrix 6.0 array. After quality control 574922 and 575010 SNPs in CAMP and CARE respectively, were tested with FBAT. No main effects genome-wide significant associations were found. We prioritized candidate pairs of SNPs for MB-MDR epistasis screening using Biofilter leading to 7632 SNPs for CAMP and 7603 SNPs for CARE. The most significant pair-wise interaction was identified between SNPs from loci 7p21.1 and 12q23.3 influencing eosinophil level in asthmatics. [less ▲]

Detailed reference viewed: 65 (8 ULg)
See detailEvidence of a verbal overshadowing effect in children
Vanootighem, Valentine ULg; Brédart, Serge ULg; Dehon, Hedwige ULg

Poster (2011, August 01)

The report of verbal descriptions of a culprit by adult witnesses may impair their later identification ability, a phenomenon known as the “verbal overshadowing effect (VO)” (Schooler & Englster-Schooler ... [more ▼]

The report of verbal descriptions of a culprit by adult witnesses may impair their later identification ability, a phenomenon known as the “verbal overshadowing effect (VO)” (Schooler & Englster-Schooler, 1990). In spite of a large body of literature on the suggestibility of children testimony, only one study has examined whether descriptions also impaired children’s identification abilities in a single group of children (8-9 years old) and no evidence of VO was found (Memon & Rose, 2002). However, some procedural details were not controlled in this experiment and the absence of a control adult group did not allow determining whether the procedure used was able to induce a VO effect. Hence, 2 experiments were conducted on several groups of children (7-8, 10-11, 13-14 years old) and adults to determine the influence of development on the VO effect. Overall, a VO effect on face identification was found in both experiments. The quality and influence of descriptors across the ages were also examined. [less ▲]

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See detailVHHs as model proteins to investigate amyloid fibril formation: effect of seeding and cross-seeding on the stability of fibrils
Chavignon, Chloé ULg; Pardon, Els; Wyns, Lode et al

Poster (2011, August)

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidoses are known so far, amongst ... [more ▼]

The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidoses are known so far, amongst which Alzheimer's disease, type II diabetes and immunoglobulin amyloidosis [1]. Although the mechanism of amyloid fibril formation at the molecular level is not yet completely understood, it has been shown that the capacity to form amyloid fibrils in vitro is an intrinsic property of all polypeptide chains [1]. The choice of model proteins to investigate the aggregation process in vitro is therefore not restrained to proteins involved in amyloidoses but can be settled on a wide variety of proteins. In this study, we have chosen to investigate the mechanism of amyloid fibril formation by two variable domains of camelid heavy-chain antibodies (referred to as VHHs or nanobodies), cAb-HuL6 and cAb-BcII10, for which variants with mutations located at the disulfide bond [3,4] and the CDRs [3] are available. Characterisation of the aggregating properties of these mutants will allow the investigation of the impact of these structural elements on the process of fibril formation. In order to determine conditions in which cAb-HuL6 and cAb-BcII10 are more susceptible to form amyloid fibrils, heat-induced unfolding experiments at several pHs have been monitored by intrinsic fluorescence and circular dichroism. Then, aggregation experiments have been performed in the selected conditions and the presence of amyloid fibrils has been acknowledged by thioflavineT fluorescence experiments and electron microscopy. We will discuss the kinetics of aggregation obtained in the absence and the presence of seeding/cross-seeding and the stability of the formed fibrils. [1] Chiti and Dobson, Annu. Rev. Biochem., 75, 2006, 333-366 ; [2] Dumoulin et al., Protein Sci., 11, 2002, 500-515 ; [3] Saerens et al., J. Mol. Biol., 352, 2005, 597-607 ; [4] Saerens et al., J. Mol. Biol., 377, 2008, 478-488. [less ▲]

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See detailAssessing IT Innovations in US State Governments: a research framework
Markus, M. Lynne; Bui, Quang Neo; Jacobson, Dax D. et al

Poster (2011, August)

Detailed reference viewed: 41 (10 ULg)
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See detailExopolysaccharides production by selected bacteria
Valepyn, Emmanuel ULg; Paquot, Michel ULg; Nys, Joël

Poster (2011, August)

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See detailEffet de la technique vocale sur la justesse en voix chantée
Larrouy, Pauline ULg; Morsomme, Dominique ULg

Poster (2011, August)

Selon Dalla Bella et al. (2007, 2009), la population générale est capable de chanter juste une chanson populaire lorsque le tempo de la production est lent. Le chant est une activité universelle, présente ... [more ▼]

Selon Dalla Bella et al. (2007, 2009), la population générale est capable de chanter juste une chanson populaire lorsque le tempo de la production est lent. Le chant est une activité universelle, présente dès le plus jeune âge, mais reste une tâche complexe mobilisant des habiletés physiques, cognitives et physiologiques. En travaillant leur voix, les chanteurs professionnels, tels des instrumentistes, renforcent ces habiletés et sont capables de modifier la qualité de leur production vocale. Dans cette étude, nous examinons l’effet de cette expertise vocale et plus particulièrement de la technique “lyrique” enseignée aux chanteurs d’opéra sur certaines caractéristiques telles que la hauteur absolue et le tempo. En ce qui concerne la justesse, les techniques d’analyse acoustique mises au point nous permettent d’observer les erreurs possibles en production vocale (Schön et al., 2004) dans un contexte mélodique. Nous avons constitué un groupe contrôle de 63 non chanteuses et un groupe expérimental de 15 chanteuses professionnelles. Les sujets ont chanté « Happy Birthday ». Les chanteuses professionnelles l’ont chanté une première fois sans technique vocale particulière (ST) et une seconde fois avec une technique vocale lyrique (AT). La partie stable de chaque voyelle a été moyennée (AudioSculpt) et analysée (OpenMusic) afin d’estimer la hauteur absolue et relative de chaque note. Les résultats montrent des différences significatives entre les groupes pour le tempo (F(2,88)=16,61, p<.001) et la hauteur absolue (F(2,88)=165,63, p<.001). Chez les non chanteuses, le nombre d’erreur diminue avec le tempo (r=.321, p=01). Chez les chanteuses professionnelles AT, nous observons le profil inverse (r=-.662, p=01) mais pas de corrélation significative chez les chanteuses professionnelles ST. Les premiers résultats confirment l’effet de la technique vocale tant sur le type d’erreur que sur l’importance de ces erreurs en contexte mélodique. Bien que nous attendions une bonne performance chez les chanteuses professionnelles AT, les résultats ne vont pas dans ce sens. Cela conduit à discuter le type d’analyse à exécuter et le choix des programmes pour le jugement de la justesse en voix chantée avec technique vocale. [less ▲]

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See detailPromoter analysis of the three HMA4 copies in the zinc hyperaccumulator Arabidopsis halleri
Nouet, Cécile ULg; Cebula, Justyna; Motte, Patrick ULg et al

Poster (2011, August)

Detailed reference viewed: 92 (7 ULg)
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See detailMonitoring and Measuring the biomass of the floating macrophytes by numerical image processing: Case duckweeds (Lemna minor) in Waste Stablisation Ponds
Tangou Tabou, Thierry ULg; Musibono Eyul"Anki, Dieudonné; Vasel, Jean-Luc ULg

Poster (2011, August)

In hot countries temperature conditions allow the use of various WSP technologies, including floating macrophytes. We studied duckweeds and they have been used for industrial or domestic waste water ... [more ▼]

In hot countries temperature conditions allow the use of various WSP technologies, including floating macrophytes. We studied duckweeds and they have been used for industrial or domestic waste water treatment. However, the vegetable carpet formed on the water surface must be managed in order to maintain a good efficiency. The duckweeds must be harvested regularly. Otherwise when the biomass is getting too large there is some overlap, mortality increases, and dead cells sink and degrade at the bottom creating a new pollution. In addition, a hazardous harvest of the lenses can involve the reduction in the capacity of purification and also support the development of algae. We tried to monitor the biomass and the environmental parameters of the system (luminosity, temperature, pH, nitrogen, phosphorus, alkalinity, COD…) as well. Indeed, the duckweed biomass measurements were carried out by many researchers (Köner and Vermaat, 1998; Caicédo et al., 2000; Rhamani and Sternberg, 1999; Edwards et al., 1992;…) according mainly to two methods, : the measurement of fresh or dry weight. It appears that these two methods present disadvantages especially for rather long experiments. If the last one is more reliable but destructive, the first on the other hand is less destructive but less accurate and not very reproducible. In the present work, we considered numerical image processing by adapted soft wares, namely: ACDsee® and Image Pro-Plus®. The purpose of this study is thus to establish the surface-biomass relationship for a possible comparison of the resulting biomasses by image and weighing [less ▲]

Detailed reference viewed: 31 (5 ULg)
See detailExpression of the metal homeostasis gene FRD3 in two Arabidopsis species
Charlier, Jean-Benoît ULg; Polese, Catherine ULg; Krämer, Ute et al

Poster (2011, August)

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See detailEndotoxin-induced alterations in renal oxygen consumption: an ESR oximetry study
Quoilin, Caroline ULg; Grammenos, Angeliki ULg; Gallez, Bernard et al

Poster (2011, August)

The kidney, one of the most injured organs in critically ill patients, is faced with unique challenges for molecular oxygen regulation. Recent research activities in the pathophysiological mechanism of ... [more ▼]

The kidney, one of the most injured organs in critically ill patients, is faced with unique challenges for molecular oxygen regulation. Recent research activities in the pathophysiological mechanism of acute renal injury (ARI) emphasize the central role of hemodynamic and inflammatory events in septic shock. More particularly, two mechanisms have been postulated to explain the inability of the injured kidney to extract oxygen: tissue hypoxia and cellular energetic metabolism dysfunction. The present investigation was carried out to characterize the effects of bacterial endotoxin on the oxygen consumption of human tubular proximal cell line (PTC) by using the very sensitive electron spin resonance oximetry method. Oxygen consumption was shown to decrease quite markedly in cells treated with lipopolysaccharide (LPS) from 16.52 ± 2.51 (n=6) in the control group to: 12.94 ± 2.62 (n=3) in the short incubation time group (6h) and 10.86 ± 2.20 (n=3) in the long incubation time group (18h). This decrease in oxygen consumption in renal cells after LPS challenge may be in relation with a metabolic down-regulation. Renal energetic are deranged in sepsis not just because O2 delivery is impaired but perhaps also because the ability of cells to utilize available O2 is compromised. [less ▲]

Detailed reference viewed: 84 (20 ULg)
See detailC FIBERS - MG MATRIX COMPOSITES PRODUCED BY SQUEEZE CASTING AND FRICTION STIR PROCESSING: Microstructure & Mechanical Behaviour
Mertens, Anne ULg; Simar, Aude; Delannay, Francis

Poster (2011, August)

Mg matrix composites appear as suitable competitors to Al alloys, as they allow for a significant weight reduction while exhibiting potentially comparable mechanical properties. A large variety of ... [more ▼]

Mg matrix composites appear as suitable competitors to Al alloys, as they allow for a significant weight reduction while exhibiting potentially comparable mechanical properties. A large variety of processing routes have already been considered for their production, including 'liquid state' processes such as squeeze casting. However these techniques necessitate a very careful control, simultaneously, of the solidification process, the wetting behaviour and the possible interfacial reactions between the reinforcements and the molten metal. And this is more particularly true in the case of Mg matrix composites with carbon fibers reinforcements, as Mg alloys are known to exhibit a poor wetting behaviour on carbon substrates. As a consequence, 'solid state' processing routes such as friction stir processing, that has been developed more recently, provide promising alternatives for the production of C-Mg composites. In the present work, Mg-Al-Zn alloys have been reinforced with carbon fibers using squeeze casting and friction stir processing. The microstructures and mechanical behaviour of composites produced by both processes have then been characterised and compared to those of non-reinforced samples, with a particular attention to the damage mechanisms prevailing in the composite materials. As a result, both processes have been compared in terms of the properties of their final products and hence their ability to produce sound composites. [less ▲]

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See detailAttraction of wireworms towards root-emitted volatile organic compounds of barley
Barsics, Fanny ULg; Latine, Rémi ULg; Haubruge, Eric ULg et al

Poster (2011, August)

Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for ... [more ▼]

Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for environmental issues gave birth to new needs in terms of management. In order to enhance integrated management of this pest, we work on the chemical ecology of Agriotes sordidus Illiger. We focus on its interaction with barley roots, mediated by volatile organic compounds (VOCs). Our experimentations are based on behavioural responses of wireworms in olfactometric devices and their statistical relevance [less ▲]

Detailed reference viewed: 49 (12 ULg)
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See detailHost location by the parasitoid, Nasonia vitripennis Walker (Hymenoptera : Pteromalidae)
Frederickx, Christine ULg; Durieux, Delphine ULg; Verheggen, François ULg et al

Poster (2011, July 24)

Successful reproduction for parasitoids encompasses a series of behavioral steps commonly defined as host-habitat location, host location, host acceptance and host regulation. Successful host location ... [more ▼]

Successful reproduction for parasitoids encompasses a series of behavioral steps commonly defined as host-habitat location, host location, host acceptance and host regulation. Successful host location, where resources are patchily distributed within the environment, is dependent on the information value of stimuli used in the host location process. Chemical cues produced by either the host itself, products derived from the host play an important role in host location. This study investigated the role of odorant cues used during host location by the generalist parasitoid, Nasonia vitripennis Walker. N vitripennis is a common parasitoid of Dipteran pupae found in association with decaying carrion. The biological activity of eight of the volatile molecules constituting the odour of pupae were tested on the searching behavior of parasitoid females through chemoecological approache: olfactometry bioassays. [less ▲]

Detailed reference viewed: 74 (15 ULg)
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See detailMorphosyntactic disorders as a consequence of categorization difficulty in children with specific language impairment
Leroy, Sandrine ULg; Parisse, Christophe; Duquet, Adèle et al

Poster (2011, July 21)

Detailed reference viewed: 79 (11 ULg)
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See detailGlycine receptor activation influences early cortical development
Avila Macaya, Ariel Salvatore ULg; Nguyen, Laurent ULg

Poster (2011, July 14)

The strychnine-sensitive glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily. In the adult, the GlyR is known to mediate fast inhibitory neurotransmission in the spinal cord ... [more ▼]

The strychnine-sensitive glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily. In the adult, the GlyR is known to mediate fast inhibitory neurotransmission in the spinal cord and in the brainstem. The GlyR has also been described in the embryonic cortex after embryonic day 19 (E19) (Flint et al., 1998) where it could participate in developmental processes, but its presence at earlier stages has not been documented. Since other neurotransmitter systems, i.e. GABA and its receptors, are known to be potent signals that control corticogenesis (Nguyen et al., 2001; Ik-Tsen et al., 2007), we wondered if glycine and its GlyR could also fulfill such a function. In this study, we analyze GlyR expression and its physiological function in the early development of the cortex using in vitro cultures of embryonic day 13 slices, patch-clamp and immunocytochemistry. [less ▲]

Detailed reference viewed: 32 (4 ULg)
See detailThe multiple spots of the Ganymede footprint
Bonfond, Bertrand ULg; Hess, S.; Grodent, Denis ULg et al

Poster (2011, July 11)

Detailed reference viewed: 18 (8 ULg)
See detailThe Ultraviolet Spectrograph (UVS) on Juno
Gladstone, G. R.; Persyn, S.; Eterno, J. et al

Poster (2011, July 11)

Detailed reference viewed: 37 (5 ULg)
See detailAuroral signatures of injections in the magnetosphere of Saturn
Radioti, Aikaterini ULg; Roussos, E.; Grodent, Denis ULg et al

Poster (2011, July 11)

Detailed reference viewed: 24 (14 ULg)
See detailCassini UVIS Observations of Varying Auroral Emissions on Saturn's Night Side
Pryor, W.; Esposito, L.; Jouchoux, A. et al

Poster (2011, July 11)

Detailed reference viewed: 13 (3 ULg)
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See detailEstimating daily yield and content of major fatty acids from single milking: First approach
Arnould, Valérie ULg; Froidmont, Eric; Nguyen, Nam et al

Poster (2011, July 11)

Detailed reference viewed: 36 (18 ULg)
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See detailOxylipins in plant defense against aphids
Avila, Carlos; Arevalo-Soliz, Milenka; Fauconnier, Marie-Laure ULg et al

Poster (2011, July 11)

Detailed reference viewed: 46 (8 ULg)
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See detailAphid - phytovirus interactions: investigation of virus binding mechanisms in insect vectors by lectin use and proteomic approach
Yattara, Almouner Ag Alhamis ULg; Véronique Genin, Eric Haubruge; Francis, Frédéric ULg

Poster (2011, July 11)

Aphids are well known for their role in virus transmission to host plant. In some cases, the virus is transmitted from plant to plant simply attached to the cuticule of the mouthparts or the forget. For ... [more ▼]

Aphids are well known for their role in virus transmission to host plant. In some cases, the virus is transmitted from plant to plant simply attached to the cuticule of the mouthparts or the forget. For other, circulative virus transmission based on virion internalization through the aphid gut followed by transfer to salivary glands and finally to next plant during aphid feeding is required. In both situations, presence of receptor components through the digestive tract of the aphids is needed for virus binding and further transmission to next plants even if not localized at the same place. In order to investigate the specific binding of virus on particular aphid receptors, two aphid-virus models were selected to be tested using several lectins showing differential sugar binding specificities. Virus transmission efficacy assays with Myzus persicae and potato virus but also Acyrthosiphon pisum and pea enation mosaic virus were performed using a range of lectins to assess the potential competition of lectins and virus. Some interesting lectins were found to reduce the virus transmission with a 2 fold factor showing potential use of lectin in virus spread control. The aphids were also investigated by a proteomic approach using a two Dimension-Differential in Gel Electrophoresis (2D-Dige) coupled with mass spectrometry to determine the aphid proteins involved in virus transmissions. Head or digestive tubes of aphids were collected and investigated for non persistant or persistant virus models respectively. Differential abilities of aphids to transmit the selected virus models are discussed in relation with lectin affinity specificity and investigated aphid proteins found to be involved in vector-virus interactions. The application of lectin as potential way to reduce virus transmission by aphids will also be developed. [less ▲]

Detailed reference viewed: 86 (14 ULg)
See detailModel of the Jovian magnetic field topology constrained by the Io auroral emissions
Hess, S.; Bonfond, Bertrand ULg; Grodent, Denis ULg et al

Poster (2011, July 11)

Detailed reference viewed: 4 (1 ULg)
See detailVariability of methane in the epilimnion of Lake Kivu
Borges, Alberto ULg; Abril, Gwenael; Delille, Bruno ULg et al

Poster (2011, July 11)

Detailed reference viewed: 14 (1 ULg)
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See detailEvaluating radical conservation futures: strategies for assessing the potential for Eurasian lynx in the Benelux countries
Bouyer, Yaëlle ULg; Poncin, Pascal ULg; Beudels-Jamar, Roseline et al

Poster (2011, July 08)

Tolerance of anthropic environment has allowed large carnivores to recolonize and to be reintroduced within Europe in the last forty years. This indicates that, even in areas where the return of large ... [more ▼]

Tolerance of anthropic environment has allowed large carnivores to recolonize and to be reintroduced within Europe in the last forty years. This indicates that, even in areas where the return of large carnivores seems unlikely, their presence and establishment is possible, even in highly modified environments. In the current context of the return of large carnivores in Europe, there is a need for scientific conservation tools to assess the full potential and consequences of the return of large carnivores in Europe, and in particular to evaluate potential impact on game and livestock. Today, the return of the Eurasian Lynx (Lynx lynx) in areas where it has been absent for long periods is becoming increasingly probable, and this requires further investigations. Three main lines of research will be particularly important: the use of habitat by Eurasian Lynx, the effect of habitat on predation behavior, and potential sources of conflict with human populations. The research project will address fine scale habitat use, the effect of prey‘s spatial variation on Lynx predation behavior, the use of different statistical methods to estimate Lynx‘s viability in various habitats and landscape, and a review of current conflicts throughout Europe. Results should allow better understanding of the Lynx redeployment potentials in lowland Western Europe [less ▲]

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See detailProduction de cartes généralisées à partir de la Carte Numérique des Sols de Wallonie
Legrain, Xavier ULg; Demarcin, Pierre ULg; Mokadem, Abdel Illah et al

Poster (2011, July 07)

La Belgique est parmi les premiers et rares pays à s’être dotée, entre 1947 et 1991, d’une carte des sols à grande échelle (1/20 000) sur l’ensemble de son territoire. Entretemps, le développement actuel ... [more ▼]

La Belgique est parmi les premiers et rares pays à s’être dotée, entre 1947 et 1991, d’une carte des sols à grande échelle (1/20 000) sur l’ensemble de son territoire. Entretemps, le développement actuel des applications numériques d’une part, une prise de conscience accrue de l’importance de préserver les sols d’autre part, ont amené la Région wallonne à entreprendre la digitalisation de la partie wallonne de la Carte des Sols de la Belgique, pour aboutir à la production d’une couche numérique cohérente sur l’ensemble du territoire wallon, accompagnée d’une légende unique et exhaustive : la Carte Numérique des Sols de Wallonie (CNSW). Sa très grande précision, tant spatiale que sémantique, en fait un outil d’analyse puissant, mais également particulièrement complexe à manipuler. Partant du constat qu’une grande partie du territoire peut être couverte à partir d’un nombre limité d’unités cartographiques parmi les 6 000 existantes dans la CNSW, une carte des Principaux Types de Sols de Wallonie à l’échelle du 1/250 000 en a été dérivée, offrant ainsi une vision d’ensemble et une perspective d’intégration européenne. La légende fut conçue à partir de regroupements logiques des sols sur base de trois des quatre critères majeurs des unités cartographiques de la CNSW, à savoir la texture, le drainage naturel et la nature de la charge caillouteuse lorsqu’elle est présente. Adoptant des principes de dominance par l’utilisation des termes « principalement » ou « quasi-exclusivement », elle ne peut néanmoins être qualifiée de carte d’associations de sols du fait des restrictions de regroupement adoptées. Outre le rattachement de chaque plage cartographique à une des classes ainsi définies, un processus de généralisation cartographique a été effectué dans le but d’une meilleure lisibilité à l’échelle du 1/250 000. Cette carte fut utilisée comme support à la sectorisation du territoire wallon en 24 Districts de l’Espace Rural, sur base d’une analyse à caractère géomorphopédologique. Ces Districts sont à leur tour utilisés pour la réalisation de cartes des Principaux Types de Sols à l’échelle du 1/100 000, sur le même principe que la carte à l’échelle du 1/250 000. Ces diverses cartes généralisées permettent d’envisager la confrontation de la CNSW avec plusieurs gisements de données d’analyse disponibles, selon différentes échelles. En tant qu’outil d’intégration, de structuration et de cadrage de l’information pédologique, elles doivent devenir de plus en plus le fondement de Systèmes Régionaux d’Information sur les Sols et leurs propriétés. [less ▲]

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See detailInfluence of thermoelectric coupling on pacemaker activity generated by mechano-electric feedback in a one-dimensional ring-shaped model of cardiac fiber
Collet, Arnaud ULg; Desaive, Thomas ULg; Pierard, Luc ULg et al

Poster (2011, July 05)

Recently, the influence of thermal processes on electrophysiology has clearly been underlined, using a FitzHugh–Nagumo-type (FHN-type) model. When the temperature is raised, the action potential duration ... [more ▼]

Recently, the influence of thermal processes on electrophysiology has clearly been underlined, using a FitzHugh–Nagumo-type (FHN-type) model. When the temperature is raised, the action potential duration (APD) has been shown to shorten, while the action potential (AP) amplitude decreases, and the conduction velocity increases. In the present study, we investigate the effects of thermoelectric coupling on mechano-electric feedback (MEF), and more specifically, on pacemaker activity generated by MEF. To investigate these effects, thermoelectric coupling is introduced in a one-dimensional ring-shaped electromechanical model of cardiac fiber, which takes into account excitation-contraction coupling (ECC), as well as MEF. [less ▲]

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See detailEvaluation of the pedogenesis effects on the fractionation of metallic trace elements in solid wastes from industrial activities
Pineux, Nathalie ULg; Liénard, Amandine ULg; Cloots, Rudi ULg et al

Poster (2011, July 04)

This work aims at contributing to a better knowledge of the reactivity of metal-bearing phases to evaluate the risks of metal trace elements (MTE) transfers in soil-plant-water system. The high proportion ... [more ▼]

This work aims at contributing to a better knowledge of the reactivity of metal-bearing phases to evaluate the risks of metal trace elements (MTE) transfers in soil-plant-water system. The high proportion of the MTE on this type of sites seems to be mobilized only by particularly aggressive conditions but unstable bearing mineral phases have also been observed in smaller proportions. [less ▲]

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See detailA Methodology for Sampling Design dedicated to the Evaluation of Effects of Soil Type and Land Use on the Fate of Metallic Trace Elements in Contaminated Soils
Liénard, Amandine ULg; Bock, Laurent ULg; Colinet, Gilles ULg

Poster (2011, July 04)

Contaminated soils may present a risk to human health and the environment. The aim of this study is to evaluate the fate of metallic trace elements (Cd, Pb and Zn) brought to soils from atmospheric ... [more ▼]

Contaminated soils may present a risk to human health and the environment. The aim of this study is to evaluate the fate of metallic trace elements (Cd, Pb and Zn) brought to soils from atmospheric fallouts according to soil type and land use. We have developed a sampling soil strategy in order to both map soil contaminations and assess the effects of soils and land use on the mobility of MTE. [less ▲]

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See detailSimulating the Holocene re-colonization of Europe by tree species using dynamic vegetation models
Dury, Marie ULg; Lehsten, Dörte; Warnant, Pierre et al

Poster (2011, July)

Pollen and macro-fossil data collected from various localities in Europe provide the opportunity to reconstruct the speed and the routes of the post-glacial spread of European tree species. Moving from a ... [more ▼]

Pollen and macro-fossil data collected from various localities in Europe provide the opportunity to reconstruct the speed and the routes of the post-glacial spread of European tree species. Moving from a limited number of refugia at the end of the glacial period, tree species have progressively re-colonized the continent through the Holocene at seemingly species-specific migration rates. However, the relative roles of climatic fluctuations, dispersal capacities of individual species, and inter-specific competition in controlling these rates remains controversial. Here, we investigate these different aspects with two dynamic vegetation models (DVM), LPJ-GUESS and CARAIB. Transient runs of both models were performed over the Holocene, using HadCM3 GCM-reconstructed climate. Large-scale species migration at 0.5°x0.5° is represented in these models using migration rates derived from a small-scale cellular automaton, CATS. Individual tree species migration rates were pre-calculated with CATS every 1000 years over each grid cell used by the DVMs in the climatic conditions reconstructed by the GCM. In the DVMs, these migration speeds were influenced by the response to competition from other species. The DVMs were used to study the migration of one species, from its 10 kyr BP refugia, within a landscape defined by a set of other species for which no dispersal limitations are assumed. Here, we illustrate the results obtained for three wind-dispersed tree species: Abies alba, Picea abies, Fagus sylvatica and compare them to their past distributions reconstructed from pollen and macro-fossil data. [less ▲]

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See detailPhonological representations in children with SLI : a study of French vowels.
Maillart, Christelle ULg; Cazeneuve, Julia; MacLeod, Andrea

Poster (2011, July)

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See detailIncidental non linguistic regularities learning in Children with
Gabriel, Audrey ULg; Meulemans, Thierry ULg; Parisse, Christophe et al

Poster (2011, July)

Recent studies on specific language impairment (SLI) have suggested that language deficits could be partly explained by the Procedural Deficit hypothesis (PDH; Ullman & Pierpont, 2005). Tomblin et al ... [more ▼]

Recent studies on specific language impairment (SLI) have suggested that language deficits could be partly explained by the Procedural Deficit hypothesis (PDH; Ullman & Pierpont, 2005). Tomblin et al. (2007) and Lum et al. (2009; 2011) obtained data supporting this interpretation with the serial reaction time (SRT) task, as well as Evans et al. (2009) and Plante et al. (2002) with artificial grammar tasks. Recently, Gabriel et al. (2011) obtained contrasting results, showing that children with SLI were able to detect non linguistic regularities during a SRT task. The aim of this study was to assess the PDH by using a non-linguistic artificial grammar learning tasks in order to mimic real conditions of language acquisition. Twenty-three children with SLI and their typically developing (TD) peers are compared on a task in which the incidental learning sequence was presented through visual shapes via a laptop.These results confirm our previous study (Gabriel et al., 2011) by showing that children with SLI detect the rules in non-linguistic conditions. • So, contrary to results of previous studies (Evans et al., 2009; Lum et al., 2009; 2011; Plante et al., 2002; Tomblin et al., 2007), this study does not confirm the PDH in children with SLI, or at least suggests that, if present, the deficit of the procedural system in SLI is not going beyond the language system. [less ▲]

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See detailEffects of long term soil organic matter restitution mode on soil heterotrophic respiration and soil biological properties.
Buysse, Pauline ULg; Carnol, Monique ULg; Malchair, Sandrine ULg et al

Poster (2011, July)

Soil heterotrophic respiration (SHR) is the process by which CO2 is released during organic matter decomposition. It is generally expected that SHR can act as a positive feedback to global warming ... [more ▼]

Soil heterotrophic respiration (SHR) is the process by which CO2 is released during organic matter decomposition. It is generally expected that SHR can act as a positive feedback to global warming, therefore leading to more CO2 release into the atmosphere. It is thus important to better understand this process. Particularly, agricultural soils may behave as important CO2 sources that are strongly influenced by soil and crop management (e.g. organic matter restitution modes, hereafter “OM-RM”). The present study aimed at determining if, after more than 50 years of application of different OM-RM, (1) significant differences of SHR fluxes can be observed between treatments, (2) SHR responses to temperature and soil moisture content can be affected by the OM-RM and (3) the experimental design is suitable to assess potential differences between treatments. The experimental field is situated in Liroux, near Gembloux in Belgium. At that site, a long term experiment with different OM-RM runs from 1959 onwards. For the present study, three contrasted treatments were considered: (1) exportation of all residues after harvest, (2) addition of manure once every three to four years and (3) restitution of residues after harvest. SHR flux measurements were carried out manually on fourteen occasions from 2 April to 30 July 2010, using a dynamic closed chamber system. Temperature and soil moisture content at 5 cm depth were also measured manually. Results showed that after more than 50 years of OM-RM application, no significant differences could be observed between the three treatments in terms of SHR fluxes and SHR responses to temperature or soil moisture, while the soil organic carbon content did vary significantly between them. The sensitivity to temperature was quite low in all treatments, with a mean Q10 value of 1,36. Besides, SHR fluxes were seen to be more responsive to increases in soil water content than to absolute soil moisture content values. Indeed, when soil moisture content increased between two consecutive measurement dates, the ratio of the corresponding SHR fluxes was larger than 1. Particularly dry conditions in 2010 may actually have caused the fluxes to be very low, making the assessment of differences between treatments more difficult. Moreover, soil dryness is likely to be responsible for the SHR flux increases after rain events, as caused by re-solubilization of organic compounds. Also, an important spatial variability was observed, which may have obscured the assessment of potential differences between treatments. Further investigations will consist in performing a new flux measurement campaign in 2011 that will take the spatial variability issue into account, and in monitoring microbial and soil properties in the different treatments, such as microbial biomass, metabolic activity and labile carbon. [less ▲]

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See detailDigital Collaborative Studio : 4 years of practice
Safin, Stéphane ULg; Kubicki, Sylvain; Bignon, Jean-Claude et al

Poster (2011, July)

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See detailReproducibility and repeatability of upper limb landmarks palpation for junior operators
Schwartz, Cédric ULg; Fedrigo, Tatiana ULg; Bruls, Olivier ULg et al

Poster (2011, July)

In human motion analysis, bone motions are usually expressed relatively to anatomical reference frames. The anatomical reference frames are constructed thanks to the localization of bony landmarks during ... [more ▼]

In human motion analysis, bone motions are usually expressed relatively to anatomical reference frames. The anatomical reference frames are constructed thanks to the localization of bony landmarks during a static phase prior to the acquisitions. These landmarks are identified by means of palpation. Accurate comparison between subjects and studies implies good reproducibility and repeatability of the palpation process. However, all investigators don’t have a long experience in palpation. In this paper, the reproducibility and repeatability of palpation for junior investigators were measured. Results show worse reproducibility and repeatability than what is usually expected. These errors have particularly an influence on the definition of the reference frames of the arm. This study therefore emphasizes on the need of a specific training of operators working in a motion lab. [less ▲]

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See detailInvestigation on the Role of the Viral Semaphorin encoded by the A3 Gene of Alcelaphine Herpesvirus 1 in the Induction of Malignant Catarrhal Fever
Myster, Françoise ULg; Palmeira, Leonor ULg; Vanderplasschen, Alain ULg et al

Poster (2011, July)

Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried by wildebeest asymptomatically. AlHV-1 is however responsible for the development of malignant catarrhal fever (MCF) when cross-species ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV-1) is a gammaherpesvirus carried by wildebeest asymptomatically. AlHV-1 is however responsible for the development of malignant catarrhal fever (MCF) when cross-species transmitted to a variety of ruminant susceptible species. Wildebeest-derived (WD)-MCF is a fatal lymphoproliferative and degenerative disease of ruminants. Experimentally, WD-MCF can be reproduced in rabbits. A3 ORF encodes a putative semaphorin homolog protein, named AlHV-sema. Semaphorins are secreted and membrane-associated proteins characterized by a conserved ‘Sema’ domain. Initially identified as guidance factors assisting axons pathfinding during neural development, semaphorins have been shown over the last decade to have significant functions in various processes of immunoregulation. Phylogenetic analyses revealed that AlHV-sema and mammals Sema7A have a common ancestor and that AlHV-Sema has evolved independently of other viral semaphorins. Further bioinformatics analyses demonstrated that AlHV-Sema and cellular Sema7A share a highly similar tridimensional structure. In order to investigate the role of AlHV-Sema in WD-MCF induction, we used the AlHV-1 BAC clone and produced a strain deleted for A3 and a revertant strain. The strain deleted for A3 replicated comparably to the wild-type parental strain in vitro. In vivo, rabbits infected with the strain deleted for A3 developed WD-MCF with a small but significant delay compared to those infected with the parental and revertant strains. Deletion of A3 did not affect the increase of viral genomic charge over time in peripheral blood and in lymph nodes at time of death and the major histopathological lesions were present in all groups. Though infection with wild-type and revertant strains resulted in the inversion of CD8 over CD4 ratio and increased IFN- production in lymphoid tissues at time of death, both parameters were significantly reduced after infection with the A3 deleted strain. Together, these results suggest that AlHV-Sema play a role in the host response to AlHV-1 infection. [less ▲]

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See detailDo we need to standardize extraction procedures for community level physiological profiling?
Carnol, Monique ULg; Bosman, Bernard ULg; Malchair, Sandrine ULg

Poster (2011, July)

Microorganisms are essential regulators of soil functioning, as they are involved in many crucial processes such as organic matter decomposition, nutrient cycling, soil structure and fertility. Currently ... [more ▼]

Microorganisms are essential regulators of soil functioning, as they are involved in many crucial processes such as organic matter decomposition, nutrient cycling, soil structure and fertility. Currently, there is a growing interest in functional diversity, such as the number and type of substrates used for energy metabolism (CLPP-community level physiological profiling). Such metabolic diversity of heterotroph soil bacteria is frequently investigated through Biolog Ecoplates, containing 31 of the most useful carbon sources for the soil community. The metabolic diversity of soil bacteria might be an interesting biological indicator of soil quality, and also a useful tool for investigating the link between land use change, climate warming, soil carbon, microbial diversity and activity. Methods related to Biolog-CLPP reported in the literature differ in the suspension medium and extraction method, the type and density of inoculums, the inoculation procedures and conditions of incubations. For example, various combinations of extraction methods and suspension media are being used for the first bacterial extraction step. Despite such methodological differences, Biolog-CLPP data are often compared across studies. The development of a standardised method for Biolog-CLPP is however essential improving the relevance and significance of results across studies. In this work, we investigated the influence of extraction procedures on microbial extraction efficiency for further use in CLPP. The microbial extraction efficiency was tested by plate counts for a total of twelve combinations of three suspension media and four extraction methods. The experiment was performed on four soils differing in organic matter content. The aims of this study were to: • Synthesize extraction procedures used for Biolog-CLPP • Measure the effect of extraction procedures on microbial extraction efficiency (plate counts) in four soil types • Investigate a possible interaction between the suspension media and the extraction method used • Evaluate whether a standardized extraction procedure can be recommended across soil types [less ▲]

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See detailDevelopment of non invasive genetic identification methods for the Pyrenean desman (Galemys pyrenaicus) and first study of its genetic structure in France
Gillet, François ULg; Cabria Garrido, Maria Teresa; Némoz, Mélanie et al

Poster (2011, July)

The Pyrenean desman (Galemys pyrenaicus) is probably one of the most threatened European mammal species. This small insectivorous and semi-aquatic species is endemic to the Pyrenean Mountains and of the ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is probably one of the most threatened European mammal species. This small insectivorous and semi-aquatic species is endemic to the Pyrenean Mountains and of the north of the Iberic Peninsula. Many biologic aspects of this species are currently suffering from a major lack of information, particularly those concerning its genetics. Therefore the implementation of conservative efforts for the Pyrenean desman remains extremely difficult. In order to improve the knowledge of this vulnerable species and notably, to better understand its distribution area, the first aim of our research was to develop non invasive genetic identification methods based on faeces. The second aim was to have a first look at the phylogeographic structure of the Pyrenean desman. The identification methods were developed on the basis of the sequencing of a small mitochondrial DNA (cyt b) fragment as well as a RFLP method. These approaches led to the identification of the Pyrenean desman and to the differentiation of the latter from two other species living in the same type of habitat, the white-throated dipper (Cinclus cinclus) and the water shrew (Neomys fodiens). Two haplotypes were found in the studied Pyrenean populations but no phylogeographic structure could be established. A dozen of microsatellites markers were also developed during this work and 5 of these were found to be polymorphic. The results of this preliminary work tend to show a low genetic diversity for the Pyrenean desman but this result needs to be confirmed in the future with a more extended and complete study. [less ▲]

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See detailTime trends of blood leucocytes, neutrophils and plasmatic myeloperoxidase in the perioperative period of horses undergoing colic surgery.
Salciccia, Alexandra ULg; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

Poster (2011, July)

Background: Despite the recent advances in this area, colic remains a major cause of morbidity and death in horses. Neutrophilic activation and degranulation may play a key role in the postoperative ... [more ▼]

Background: Despite the recent advances in this area, colic remains a major cause of morbidity and death in horses. Neutrophilic activation and degranulation may play a key role in the postoperative complications. Activated neutrophils release enzymes like proteases and myeloperoxidase (MPO). MPO concentrations in plasma and tissue are considered as a marker of neutrophil activation. (McConnico et al. 1999; Hoy et al. 2002). When freed in the tissue, active MPO is able to oxidize, nitrate and chlorate most organic molecules (Klebanoff 2005). Objectives: The aim of this study was 1) to determine the time trends of blood leukocyte and neutrophil counts as well as of plasmatic MPO concentrations in the perioperative period of horses undergoing colic surgery and 2) to relate these time trends to the location of the pathology, the severity of postoperative complications and to the outcome of the patients. Methods: Fifty two horses undergoing colic surgery at the Equine Teaching Hospital of the University of Liege were included in this study. The location of the predominant lesion of the intestine, the severity of the postoperative complications and the outcome were recorded for each horse. Total leukocyte and neutrophil counts were performed in all of the horses while plasmatic myeloperoxidase levels were determined in 16 of them. The blood samplings were realized before and during the surgery (after correction of the intestinal lesion), during the recovery and every 4 hours during the first 4 days (from day 0 until day 4) and then every 12 hours until day 6 (150th hour after the first blood sampling) or until euthanasia. Hematologic analyses were performed at the time of sampling by use of the Medonic CA 530 (Menarini, Zaventem, Belgium). The blood was then centrifuged and the plasma was aliquoted and frozen at -20° C until assayed. MPO was assayed with a specific enzyme-linked immunosorbent assay (Equine MPO-ELISA kit, BiopTis, Liège, Belgium). A mixed model was used to analyze the time trends of leukocytes, neutrophils and MPO. All computations were done with the SAS (Statistical Analysis System) procedure Proc Mixed, with Satterthwaite degrees of freedom. The significance level was set at p=0.05. Results: The main pathology was found in the large intestine in 30 horses (58%) and the small intestine in 22 horses (42%). Forty horses (77%) survived to discharge from the clinic. Twelve horses were euthanized during the postoperative period. Their survival time varied from 0.5 day to 20 days with a mean of 7.8 days. Twenty-two horses (42%) showed none or mild complications, 12 horses (23%) showed moderate complications and 18 horses (35%) suffered from severe complications. Time trends for leukocytes and neutrophils were similar to each other (p= 0.7205) and significantly different (p< 0.0001) from the MPO time trend, which increased during the first hours, while the neutrophil time trend decreased immediately after the admission. The time trend of neutrophils was higher in large intestinal than in small intestinal pathologies and the time trend of MPO was lower in large intestinal than in small intestinal pathologies. The time trends of neutrophils were significantly different between the degrees of complications (no/mild vs moderate vs severe). For the first part of the curve, the more severe the complication, the lower is the time trend. The time trend of MPO was lower in survivors. The time trend of neutrophils in survivors was higher during the first 4 days thereafter it becomes lower than in non survivors. Conclusions: These results confirm that neutrophil counts and MPO levels undergo timely changes and that they are related to the severity of the inflammatory reaction in surgical colic cases. Knowing the kinetics of these parameters is an essential step to further determine cut-off values (with a larger group of horses) for the prognosis of horses after colic surgery. References Hoy, A., Leininger-Muller, B., Kutter, D., Siest, G. and Visvikis, S. (2002) Growing significance of myeloperoxidase in non-infectious diseases. Clin. Chem. Lab. Med. 40, 2-8. Klebanoff, S.J. (2005) Myeloperoxidase: friend and foe. J. Leukoc. Biol. 77, 598-625. McConnico, R.S., Weinstock, D., Poston, M.E. and Roberts, M.C. (1999) Myeloperoxidase activity of the large intestine in an equine model of acute colitis. Am. J. Vet. Res. 60, 807-813. [less ▲]

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See detailEvaluation of the effect of general anaesthesia on ultrasonographic images of the small intestine in horses.
Salciccia, Alexandra ULg; Gougnard, Alexandra ULg; de la Rebière de Pouyade, Geoffroy ULg et al

Poster (2011, July)

ultrasonography is currently more and more used in equine acute abdominal disease as well as in the follow-up of surgical colic patients because of its sensibility for the detection of small intestinal ... [more ▼]

ultrasonography is currently more and more used in equine acute abdominal disease as well as in the follow-up of surgical colic patients because of its sensibility for the detection of small intestinal distension. General anaesthesia is known to diminish gastrointestinal motility even if there are no clinical signs associated with it. The aim of this study was to evaluate the effect of general anaesthesia on transabdominal ultrasonographic images of the small intestine in horses undergoing routine non abdominal surgeries. The ultrasonographic exams were performed in 19 horses before and after the general anaesthesia (immediately after the recovery and then 2h, 12h and 24h after it). Each ultrasonographic exam focused on the duodenum, the jejunum and the presence of peritoneal fluid. For the jejunum, 5 locations were used: on the ventral midline just caudally to the sternum, on the left and right cranial ventral parts of the abdomen, on the left and right inguinal regions. Other parameters such as the gut sounds and the postoperative fecal output were also recorded. Anova and Chi-square tests were used for the statistical analysis. No horse showed colic signs. No significant difference was found between the pre and post anaesthetic period considering the maximal diameter of the duodenum, the maximal diameter of the jejunum on the 5 locations and the peritoneal fluid. The contractions of the duodenum were increased at the recovery compared to before the anaesthesia (p= 0,0299). The small intestine was most visible at the recovery (57,5%) and then at 2 hours after it (38,3%). It seemed that the ventral midline just caudally to the sternum and the right and left inguinal regions were the best locations to observe the jejunum (with a mean of respectively 44,6%, 39,8% and 38,5% of visualization of the jejunum).The gut sounds were very significantly decreased at the recovery (p < 0,0001) and at 2h after it (p = 0,0006). The postoperative fecal output was not decreased. In conclusion, even if general anaesthesia seems to reduce temporarily the intestinal activity (decreased gut sounds in the early post anaesthetic period), it does not cause significant distension of the small intestine (almost the small intestinal diameters were in the normal range). If an increased diameter of small intestine is observed by ultrasonography after surgery it should therefore be attributed to a pathological process and not to the anaesthesia. [less ▲]

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See detailStress negative Piétrain boars and their hybrids - Growth performances and semen quality
Do Duc, Luc ULg; Ha Xuan, Bo; Farnir, Frédéric ULg et al

Poster (2011, July)

A total of 15 boars from 3 genetic groups were used for this study, including 5 Piétrain boars (purebred Piétrain), 5 ♀Duroc × ♂Piétrain (½ Piétrain) and 5 ♀(Piétrain × Duroc) × ♂Duroc (¼ Piétrain) in ... [more ▼]

A total of 15 boars from 3 genetic groups were used for this study, including 5 Piétrain boars (purebred Piétrain), 5 ♀Duroc × ♂Piétrain (½ Piétrain) and 5 ♀(Piétrain × Duroc) × ♂Duroc (¼ Piétrain) in order to evaluate the growth performance and sperm quality of stress negative Piétrain boars and their hybrids. Testing period started at an average age of 60 days and ended at an average age of 225 days. The growth performances were weights at starting and finishing periods, average daily gain, backfat thickness, longissimus depth and lean content. The sperm quality was assessed using ejaculate volume, spermatozoon motility, sperm concentration, total number of spermatozoon in the ejaculate, rate of abnormal spermatozoon and pH of sperm. All these measurements were used to compare the genetic groups. The results showed that: The average daily gain of stress negative Piétrain boar purebred was not significantly different from Piétrain hybrids while the lean content was higher. The ejaculate volume and the spermatozoon motility of stress negative Piétrain purebred boars were higher than Piétrain hybrids but the sperm concentration and the total number of spermatozoon in the ejaculate were lower. [less ▲]

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