Subcellular localization of the Bovine Leukemia Virus (BLV) R3 and G4 accessory proteins in BLV producing cells.

Poster (2004)

The 16 kDa N-terminal fragment of the human prolactin. Design and production of minimal peptides retaining antiangiogenic and antitumoral properties

Poster (2004)

Régulation du Métabolisme des Chondrocytes par l'Oncostatine M et l'Interleukine 6

Poster (2004)

Novel surgical technique for the treatment of female stress urinary incontinence: Transobturator vaginal tape inside-out

Poster (2004)

Introduction and Objective: To describe a new, simple surgical technique for the treatment of female stress urinary incontinence (SUI) and to evaluate its feasibility. Methods: We have developed a novel ... [more ▼]

Introduction and Objective: To describe a new, simple surgical technique for the treatment of female stress urinary incontinence (SUI) and to evaluate its feasibility. Methods: We have developed a novel surgical treatment of SUI, the transobturator inside-out tension-free urethral suspension, which uses specifically designed surgical tools: a pair of stainless steel helical passers, two plastic tubes with a pointed distal end and one guide. Using these instruments, a synthetic tape is passed from underneath the urethra, through the obturator foramens, towards the thighs, without entering the pelvic region at any time during the procedure. The tubes bear a lateral opening, which allows the insertion of the helical passer into its lumen. The proximal end of each tube is attached to a non-absorbable synthetic tape. The guide acts as a shoe-horn to ease the introduction of the tubes assembled onto the helical passers from the perineal space through the obturator foramen. After perforation of the obturator membrane, a rotational movement of the helical passer around the upper part of ischio-pubic ramus allows the exit of the pointed tip of the tube and, further, of the tape at the level of the thigh on either side. The tape is positioned without tension under the junction between mid and distal urethra. Results: The procedure was carried out in 210 consecutive patients (mean age = 62 years) using the same operative protocol in all case subjects, independently of the patient’s size and weight. Mean operative time was 14 min (range = 6 to 20) in case of isolated SUI treatment. No bladder or urethra injury and no vascular (hematoma or bleeding) or neurological complication were observed. Conclusions: The results of this study indicate that our novel transobturator inside-out surgical technique for treating SUI is feasible, accurate, and quick. This technique avoids damage to the urethra and bladder and, therefore, makes cystoscopy not necessary. Further prospective studies are currently ongoing to determine the efficacy of our new surgical approach for treating SUI. [less ▲]

Study of surfactin penetration into lipid monolayers by using AFM

Poster (2004)

Recombinant antibody an its application in plant pathogen diagnosis

Poster (2004)

Genetic variability of Viola calaminaria, an endemic metallophyte.

Poster (2004)

Comparison between co-crystallisation properties of POP and OPP

Poster (2004)

Extremophiles, early Earth biosphere and exobiology

Poster (2004)

Mathematical modelling applied to caspase activation reveals a requirement for additional control

Poster (2004)

Environmental effects on the chemistry of the echinoderm skeleton

Poster (2004)

Perfluorochemicals in harbour porpoises

Poster (2004)

Contribution to the development of a biological control method against crown rot banana disease

Poster (2004)

The impact of resistograph on tree decay

Poster (2004)

Nanoscaleorganization of mixed fengycin/lipid monolayers

Poster (2004)

Trace metal variations in tissues of harbour seals (Phoca vitulina) stranded along Southern North Sea coasts

Poster (2004)

During the last decade, recurrent epizootics have affected harbour seal (Phoca vitulina) populations in the North Sea. The trace metal levels of the harbour seal in the Southern North Sea are poorly known ... [more ▼]

During the last decade, recurrent epizootics have affected harbour seal (Phoca vitulina) populations in the North Sea. The trace metal levels of the harbour seal in the Southern North Sea are poorly known. In this study, trace metal concentrations (Zn, Cu, Fe, Cd, Hg and Se) were measured in tissues (liver, kidney and muscle) of 72 harbour seals collected along the coasts of France, Belgium and Netherlands between 1994 and 2004. Hg levels were significantly higher in seals from the Southern North Sea than in seals from Norwegian waters (1) probably due to the amount of anthropogenic inputs in the North Sea. Hepatic Hg was positively correlated to hepatic Se, both increasing with the length of the seals. Such a relationship reflects an age accumulation process coupled to a detoxication mechanism involving antagonism between Hg and Se in the liver. Increasing Zn and Hg hepatic levels were observed with degrading body condition of the harbour seals, which is reflected by decreasing blubber thickness and high hepatic to total body mass ratio. Body condition was particularly poor in seals which died from infectious diseases. These observations tend to indicate a global redistribution of trace metals from muscle and blubber to liver, as a result of protein and lipid catabolism linked to disease and starvation. [less ▲]

In situ generated ruthenium N-heterocyclic carbene catalysts for atom transfer radical reactions

Poster (2004)

Ultrastructural study of the nucleolus during oogenesis in the oviparous teleost fish, Barbus barbus (L.).

Poster (2004)

Synthèse de la (+/-)-hyperaspine, alcaloïde défensif de la coccinelle Hyperaspis campestris

Poster (2004)

Dans la nature, les coccinelles ont peu de prédateurs. En effet, la plupart de ces insectes sont défendus chimiquement. Plusieurs espèces de coccinelles ont été étudiées jusqu'a présent et il a été ... [more ▼]

Dans la nature, les coccinelles ont peu de prédateurs. En effet, la plupart de ces insectes sont défendus chimiquement. Plusieurs espèces de coccinelles ont été étudiées jusqu'a présent et il a été démontré que la protection dont jouissent les coccinelles est due a la présence d'alcaloïdes répulsifs et parfois toxiques dans leur hemolymphe. Récemment, l’étude de la coccinelle Hyperaspis campestris a permis de mettre en évidence l’hyperaspine, un nouvel alcaloïde de type 3-oxaquinolizidinique. Afin de déterminer la configuration absolue de cet alcaloïde et afin d'en évaluer les activités biologiques et pharmacologiques, nous nous sommes proposés de synthétiser cette molécule. Notre schéma de synthèse se base sur une double séquence oxydation anodique - substitution nucléophile au départ de 4-pipéridinone protégée. L'énone obtenue est ensuite engagée dans une addition de Michael diastéréosélective pour former la piperidin-4-one-2,6-trans disubstituée, précurseur de l’hyperaspine. [less ▲]

Use of accuracy profiles as efficient decision tool for the validation of quantitative analytical methods

Poster (2004)

Automated determination of oxytetracycline in plasma of healthy pigs by at-line coupling of solid phase extraction to liquid chromatography

Poster (2004)

Intérêt du profil d'exactitude dans le cadre de la validation des méthodes analytiques

Poster (2004)

New fully automated method for the LC-MS/MS determination of cyproterone acetate in human plasma using restricted access material for sample clean-up

Poster (2004)

Determination of salbutamol enantiomers in human urine using anionic cyclo-dextrins in non-aqueous capillary electrophoresis

Poster (2004)

Validation d’une méthode de dosage des énantiomères du salbutamol dans l’urine par électrophorèse capillaire en milieu non aqueux en utilisant une cyclodextrine anionique comme sélecteur chiral

Poster (2004)

Impacts of plant invasions on ecosystem processes in Belgium

Poster (2004)

Diversité génétique de la oca (Oxalis tuberosa Mol.) en conservation ex situ

Poster (2004)

Exploration des processus inhibiteurs dans le vieillissement normal

Poster (2003, December 05)

Identificación y caracterización de un nuevo elemento regulatorio que involucra al factor transcripcional E2F4 en la región U5 del LTR del virus de la leucosis bovina

Poster (2003, December 01)

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E ... [more ▼]

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E. Cánepa2, K. Trono1 1Instituto de Virología, CICVyA. INTA – Castelar. 2Cátedra de Biologia Molecular, Facultad de Ciencias Exactas, UBA. Buenos Aires, Argentina. El genoma del virus de la leucosis bovina (VLB), como todo retrovirus, presenta en ambos extremos zonas denominadas Long Terminal Repeats (LTRs) de importancia en la regulación de la transcripción. Estas regiones están organizadas en tres zonas llamadas U3, R y U5. La región U3 ha sido ampliamente estudiada y se sabe que contiene los principales sitios que regulan la transcripción viral. Sin embargo, poco se sabe de las regiones R y U5 aunque ha quedado demostrado que ambas zonas contienen asimismo secuencias estimulatorias. Con el objetivo de identificar y caracterizar nuevos elementos regulatorios, en este trabajo se ha llevado a cabo el estudio de secuencias de las regiones R y U5 del LTR 5´ de BLV. El análisis de patrones de reconocimiento de secuencias consenso para factores de transcripción celulares en estas regiones, reveló la presencia de 2 posibles elementos de unión para E2F, ambos en la zona U5, denominados E2F-A y E2F-B, respectivamente. Para analizar la capacidad de unión a factores celulares de las secuencias LTRs se llevaron a cabo ensayos de cambio en la movilidad electroforética (EMSA) y supershift utilizando extractos celulares obtenidos a partir de linfocitos bovinos y empleando los oligonucleótidos U51 y U52 como sondas, donde mapean los elementos E2FA y E2FB, respectivamente. Se observó retardo por la formación de complejos ribo-proteicos en ambos casos pero solo en el caso del complejo U51-E2F pudo ser identificado el factor E2F-4 utilizando anticuerpos específicos. En resumen, estos resultados permiten identificar un nuevo sitio para el factor de transcripción E2F-4 localizado en la región U5 del LTR 5´ de BLV. Este motivo constituye un nuevo elemento regulatorio involucrado en la actividad transcripcional de genes virales y celulares dirigida por los LTRs y podría jugar un rol importante en la replicación viral. [less ▲]

Distintas variantes naturales del BLV presentan patrones diferenciales de unión a factores celulares

Poster (2003, December 01)

DISTINTAS VARIANTES NATURALES DEL BLV PRESENTAN PATRONES DIFERENCIALES DE UNIÓN A FACTORES CELULARES. S. Rodriguez1, C. Varone2, M. Mozgovoj1, C. Gómez1, M.J. DusSantos1, A. Wigdorovitz1, E. Cánepa2, K ... [more ▼]

DISTINTAS VARIANTES NATURALES DEL BLV PRESENTAN PATRONES DIFERENCIALES DE UNIÓN A FACTORES CELULARES. S. Rodriguez1, C. Varone2, M. Mozgovoj1, C. Gómez1, M.J. DusSantos1, A. Wigdorovitz1, E. Cánepa2, K. Trono1 1Instituto de Virología, CICVyA, INTA – Castelar. 2Cátedra de Biologia Molecular, Facultad de Ciencias Exactas, UBA. Buenos Aires, Argentina. La relación entre el genotipo y las formas de manifestación patogénica está demostrada para ciertos Retrovirus, y las zonas regulatorias Long Terminal Repeats (LTR) han sido descriptas, entre otros factores, como portadoras de determinantes de patogenia. En el caso del Virus de la Leucosis Bovina (VLB), estudios previos de secuencias llevados a cabo en nuestro laboratorio sobre las zona R-U5 de las regiones genéticas provirales LTR aisladas de 36 animales infectados naturalmente y con diferentes formas de evolución revelaron la presencia de 4 cambios fijos y característicos de provirus aislados de linfosarcoma (LS)(100%), 1 en la región R y 3 en U5. Estas modificaciones están ausentes en la mayoría (16/22) de los provirus aislados de formas asintomáticas (AL/PL). La alta asociación entre los cambios de nucleótidos y las formas de patogenia demostró la existencia de una secuencia de nucleótidos consenso y propia de los aislados de LS en la región U5 del LTR del genoma proviral de VLB. Con el objetivo de analizar si existe una capacidad diferencial de reconocimiento de factores de regulación de la transcripción celular en la célula hospedadora de las secuencias LTRs de aislamientos de distintas formas de patogenia, se utilizaron ensayos de cambio en la movilidad electroforética (EMSA). La evaluación conjunta de los resultados demuestra que existirían diferencias específicas de unión de la región U5 a proteínas del complejo de regulación de la transcripción celular denominadas E2F en relación con las diferentes presentaciones de esta patología. Estos hallazgos aportan datos en relación con la caracterización de esta co-variación de nucleótidos como marcadores de patogenia del VLB, así como también representan un adelanto en el conocimiento de la patobiología del BLV. [less ▲]

Identification of specific biomarkers related to the effects of pollutants on the immune system of marine mammals

Poster (2003, December)

Antithrombotic activity of BM-573, a novel thromboxane modulator, in rat arterial thrombosis model, pig myocardial infarction model and its effect on bleeding time

Poster (2003, December)

BM-613, a new thromboxane A2 antagonist, is characterized by a preferential activity on platelet thromboxane A2 receptors

Poster (2003, November 22)

Effects of recently developed 6-substituted 3-bromophenyl 2-oxo-2H-1-benzopyran-3-carboxylate derivatives on HT1080 cell invasion in vitro

Poster (2003, November 22)

La voie transobturatrice de dedans en dehors : considérations anatomiques.

Poster (2003, November 19)

Typologie floristique des cours d’eau luxembourgeois

Poster (2003, November 08)

Étude rétrospective des causes médicales d’intolérance à l’effort chez le cheval.

Poster (2003, November)

Design and evaluation of new reinforced hydrogel membranes for biomaterial purposes

Poster (2003, October 23)

The skin compatibility of new cyclodextrin derivatives: the phospholipidyl-cyclodextrins

Poster (2003, October)

The scavenging crustacean guild in the Antarctic shelf and deep-sea communities: composition, distribution and eco-functional role

Poster (2003, October)

Application of X-ray microtomography to follow cracks development during convective drying of deformable materials

Poster (2003, September)

Effect of the inclusion time of extruded linseed supplementation before slaughter on n-3 fatty acids enrichment of chicken meat.

Poster (2003, September)

Blending of low erucic rapeseed oil, hydrogenated low erucic rapeseed oil and anhydrous milk fat in the preparation of shortenings

Poster (2003, September)

Universal Automated Extraction and Clean-up System for Dioxin and PCB analysis in Food and Feed

Poster (2003, September)

Isolation of bacteriocin-producing lactic acid bacteria from fermented fish product in Senegal.

Poster (2003, August)

Relationship between structure and biological properties of different lipopeptides produced by B. Subtilis.

Poster (2003, August)

2-oxepane-1,5-dione: a precursor of a novel class of versatile semicrystalline biodegradable (co)-polyesters

Poster (2003, July 01)

2-oxepane-1,5-dione (OPD) was synthesized by Baeyer-Villiger oxidation of 1,4-cyclohexanedione and ring-opening polymerized by 1-phenyl-2-propanol / tin octoate with formation of a semicrystalline ... [more ▼]

2-oxepane-1,5-dione (OPD) was synthesized by Baeyer-Villiger oxidation of 1,4-cyclohexanedione and ring-opening polymerized by 1-phenyl-2-propanol / tin octoate with formation of a semicrystalline polyester of high melting temperature (Tm: 147°C). A crystallographic study showed that the polyester is orthorombic in the P212121 space with two molecules extended along the c-axis as is the case for poly(ε-caprolactone) (PCL). Dibutyltin dimethoxide and 2,2-dibutyl-2-stanna-1,3-dioxepane (DSDOP) proved to be efficient initiators for the random copolymerization of ε-caprolactone (εCL) and OPD. Random distribution of the comonomer units was confirmed by DSC, 1H and 13C NMR analyses. A single Tm was observed, which varies regularly and monotonically with the OPD content as result of a cocrystallisation phenomenon. The single glass transition temperature (Tg) obeys the Fox equation. It is noteworthy that a copolymer containing 30 mol % OPD exhibits a Tm of 90°C. It is stable up to 140°C under nitrogen for one hour, such that processing by extrusion is quite possible. The OPD decomposition is maximum at 223°C for a heating rate of 10°C/min. The mechanism of thermal degradation was investigated and cleared up. The ketone increases the copolymer hydrophilicity and thus sensitivity to hydrolysis. The mechanical properties are quite reminiscent of PCL of comparable molecular weight. Quite interestingly, OPD imparts sensitivity to photo-cross-linking to this novel class of materials. [less ▲]

Comparative phylogeography of Apodemus sylaticus (Rodentia, Muridae) and one of its diret and specific endoparasite, Heligmosomoides polygyrus (Nematoda, Heligmosomoidea) in the western Palearctic region

Poster (2003, July)

Radiosynthèses d'antagonistes des récepteurs 5-HT1A.

Poster (2003, June 13)

Mise au point de sondes pour l'étude du système sérotoninergique par tomographie à émission de positons (TEP)

Poster (2003, June 13)

La quantification de la vitesse de biosynthèse de la sérotonine ainsi que l'étude in vivo chez l'homme sont, du fait de l'implication cruciale des neurones sérotoninergiques dans de nombreuses fonctions ... [more ▼]

La quantification de la vitesse de biosynthèse de la sérotonine ainsi que l'étude in vivo chez l'homme sont, du fait de l'implication cruciale des neurones sérotoninergiques dans de nombreuses fonctions et pathologies cérébrales, des domaines d'études fondamentaux pour lesquels la TEP constitue un outil de choix. Ces différentes expérimentations peuvent être conduites suivant deux optiques: (i) emploi d'un traceur capable de suivre la chaîne métabolique du tryptophane conduisant à la sérotonine tout en évitant les autres voies métaboliques. (ii) utilisation d'un inhibiteur de la tryptophane hydroxylase (TPOH), enzyme limitante du processus. [less ▲]