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See detailOptoacoustic specific detection of prostate cancer using functionalized gold nanorods
Schol, Daureen ULg; Fleron, Maximilien ULg; Greisch, Jean-François et al

Poster (2008, March 12)

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See detailSIGHTING FREQUENCY AND PHOTO-IDENTIFICATION OF BOTTLENOSE DOLPHINS (TURSIOPS TRUNCATUS) ALONG THE COAST OF BAHÍA SAN ANTONIO, PATAGONIA, ARGENTINA
Holsbeek, Ludo; Cammareri, Alejandro; Failla, Mauricio et al

Poster (2008, March 10)

The sighting frequency (SF), site fidelity, home-range and group composition of bottlenose dolphins (Tursiops truncatus) were assessed during a photo-identification study in 2006-2007. This study aims to ... [more ▼]

The sighting frequency (SF), site fidelity, home-range and group composition of bottlenose dolphins (Tursiops truncatus) were assessed during a photo-identification study in 2006-2007. This study aims to provide first data on this unknown austral bottlenose dolphin population to answer to their increasing conservation needs. Data and pictures were collected during 132 surveys with an average observation effort of 2.8h (SD=1.6) per survey. All clear pictures were analysed using the automatic identification systems FinEx and FinMatch (EuroPhlukes Initiative). The SF was obtained dividing the number of sightings by the amount of effort. Site-fidelity was estimated using the Capture Mark Recapture method and the degree of residency by the re-identification frequency following Culloch (2004). In total, 377.4h were spent searching for bottlenose dolphins of which 57.3h were spent in the presence of 126 dolphin groups divided in 105 sightings. The SF was 0.28/h with and average duration of 43.2min/sighting (SD=0.76). A total of 43 dolphins were classified into an identification catalogue. These recognizable dolphins were re-identified up to 10 days with 44% (n=43) showing a degree of residency (resighting frequency (RF)¿4). The average group size was 6.1 (SD=7.9) ranging from one to fifty animals. Group size increased significantly with the presence of calves and with the distance from the coast. Nevertheless, all dolphins were seen in inshore waters ¿20m deep. The most frequent group formation was loose (34.5%), variable (29.8%), disperse (20.2%) and tight (15.5%). At least 6 identified dolphins, including one mother with her calf, were photographed in the mouth of the Rio Negro 250km from the study area indicating that their home-range is far from limited to Bahia de San Antonio. Data indicate that it concerns a resident but yet unknown population of bottlenose dolphins with a high commercial potential and an urgent need of conservation measurements. [less ▲]

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See detailExtraction of ferulic acid, a natural antioxidant, for potential agro-industrial applications.
Barberousse, Hélène; Roiseux, Olivier; Deroanne, Claude et al

Poster (2008, March 05)

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See detailA structured expert judgement study on Salmonella spp. in pork: analyses of different weighting schemes.
Boone, Idesbald; Van der Stede, Y.; Bollaerts, K. et al

Poster (2008, March)

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See detailNorovirus bovins isolés en Belgique en 2007 et investigation de leur potentiel zoonotique par l’étude des interactions virus-celllules
Mauroy, Axel ULg; Scipioni, Alexandra; Mathijs, Elisabeth et al

Poster (2008, March)

Appartenant à la famille des Caliciviridae, les norovirus sont des virus non enveloppés. Leur génome est composé d’un ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Trois cadres ... [more ▼]

Appartenant à la famille des Caliciviridae, les norovirus sont des virus non enveloppés. Leur génome est composé d’un ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Trois cadres ouverts de lecture (ORFs) y sont décrits et l’ORF 2 code pour l’unique protéine composant leur capside. Les norovirus infectent l’homme et les animaux (bovins, porcins, murins). Chez l’homme, ils sont des agents majeurs de gastroentérite sporadique ou épidémique d’origine souvent alimentaire. Chez le bovin, ils seraient également les agents d’entérite bénigne bien qu’à l’heure actuelle aucune épidémie à norovirus n’ait été décrite chez cette espèce. La voie d’infection des norovirus est habituellement oro-fécale, ils sont très résistants dans l’environnement et une infection peut survenir même avec une très faible dose infectieuse. Les norovirus humains et animaux sont relativement proches génétiquement et coexistent parfois de manière très étroite dans nos pays d’Europe du nord. Il est donc logique d’envisager le risque zoonotique lié aux norovirus animaux et plus particulièrement celui lié aux norovirus bovins. Ces derniers sont considérés comme endémiques dans des pays proches de la Belgique et une forte séroprévalence apparente a été montrée dans ce pays. Ce travail avait pour but l’étude moléculaire des souches de norovirus bovins ayant circulé au cours de l’année 2007. L’investigation préliminaire de leur potentiel zoonotique a également été étudiée au travers des interactions virus-cellules. Une banque d’échantillons de matières fécales bovines en provenance d’un laboratoire d’analyse et de diagnostic vétérinaire installé en Région Wallonne (ARSIA) a été constituée tout au long de l’année. Un diagnostic rapide par RT PCR a été effectué pour détecter des séquences de norovirus bovins des deux génotypes décrits actuellement. Les couples d’amorces utilisés, JV12-13, BEC et CBECu s’hybridaient dans les régions codant pour la polymérase virale et dans le début de l’ORF2, régions assez conservées. Ces séquences ont été analysées comparativement à celles isolées dans les années précédentes. Parallèlement, des pseudoparticules d’une souche de norovirus bovin (B309) et d’une souche de norovirus humain (HV) ont été produites comme décrit précédemment avec de légères modifications. Leur concentration protéique a été obtenue par BCA et des lapins ont été immunisés avec ces antigènes. Trois injections de 25 µg d’antigènes dilués dans du PBS et complétées avec les adjuvants complet et incomplet de Freund ont été réalisées. La production d’un sérum hyperimmun dirigé contre la protéine de capside des norovirus a été contrôlée par ELISA. Les pseudoparticules ont ensuite été utilisées pour des études de liaisons sur différents types cellulaires dont les Caco2, cellules connues pour exprimer à leur surface des oligosaccharides proches de ceux des systèmes ABO et de Lewis, ces oligosaccharides étant impliqués comme récepteurs cellulaires pour de nombreux norovirus humains. L’attachement des pseudoparticules a été mis en évidence par immunofluorescence indirecte en utilisant les sérums polyclonaux et un anticorps secondaire anti-lapin couplé à l’Alexa fluor 488. Des séquences de norovirus bovins ont pu être identifiées dans les prélèvements de matières fécales bovines tout au long de la période de constitution de la banque, ces séquences étant proches de celles des norovirus bovins de génotype 2. Une prévalence apparente dans les cheptels bovins de Wallonie a put être déterminée. Au cours de premiers tests réalisés, si les pseudoparticules de HV se sont liées aux cellules Caco2, aucun attachement des pseudoparticules du norovirus bovin à ces mêmes cellules n’a pu être démontré. Nous avons donc montré que les norovirus sont largement répandus en Belgique et leur diagnostic tout au long de la période d’échantillonnage prouve un certain caractère endémique de ces virus en Belgique ; cette constatation rejoignant celles en provenance de pays proches (Grande Bretagne, Allemagne) et de séroprévalence. De ce fait, ils pourraient constituer un risque zoonotique, risque qui pourrait être cependant pondéré par les études préliminaires d’interaction virus-cellules. Des études plus approfondies ont besoin d’être conduites à ce sujet. [less ▲]

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See detailFrom Bouncing to boxing
Terwagne, Denis ULg; Gilet, Tristan ULg; Vandewalle, Nicolas ULg et al

Poster (2008, March)

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See detailOptimization of the Production of the Amyloidogenic Variants of Human Lysozyme
Menzer, Linda ULg; Tocquin, Pierre ULg; Dony, Nicolas et al

Poster (2008, February 16)

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See detailREconstruction of COLOUR scenes
Sirjacobs, Damien ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2008, February 12)

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See detailTalking while walking in multiple sclerosis: Increased difficulty of walking abilities, deficit of working memory, or both?
DELRUE, Gaël ULg; Gryglewicz, Céline; Leonard, Yves et al

Poster (2008, February 02)

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See detailInventaire des dégâts d'écorcement en peuplements résineux
Gheysen, Thibaut ULg; Lejeune, Philippe ULg; Arnal, Delphine et al

Poster (2008, January 25)

Une gestion des populations de cervidés en harmonie avec la forêt et les multiples fonctions qu’elle est aujourd’hui amenée à remplir nécessite notamment de pouvoir apprécier de manière objective et ... [more ▼]

Une gestion des populations de cervidés en harmonie avec la forêt et les multiples fonctions qu’elle est aujourd’hui amenée à remplir nécessite notamment de pouvoir apprécier de manière objective et précise la pression exercée par la grande faune sur les zones davantage réservées à la production ligneuse. Les recensements par observations directes constituent souvent la principale source d’informations à partir desquelles il est habituel d’estimer les populations de cervidés et d’établir les plans de gestion de ces populations. Ces méthodes présentent cependant le double désavantage d’être coûteuses en moyens humains et de produire des résultats dont la précision et l’objectivité laissent parfois à désirer. Aussi, certains auteurs préconisent-ils de remplacer ou, à tout le moins, de compléter ces recensements par une observation des dégâts frais affectant la végétation ligneuse. [less ▲]

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See detailPolar lipids analysis from the MFGM by SPE and HPLC-ELSD
Bodson, Pascal ULg; Danthine, Sabine ULg; Blecker, Christophe ULg et al

Poster (2008, January 23)

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See detailOptiVal: Vers de nouveaux outils de gestion pour les éleveurs laitiers wallons, Prédire la réussite à l’insémination
Laloux, Laurent; Bastin, Catherine ULg; Glorieux, Géry et al

Poster (2008, January 23)

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See detailLa membrane du globule gras du lait (MFGM) : une composition et une structure originale.
Bodson, Pascal; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

Poster (2008, January 23)

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See detailEffects of intravenous lidocaïne in healthy adult horses.
Amory, Hélène ULg; Lopez Arevaldo, Diégo; Maes, An et al

Poster (2008, January)

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See detailSoil physical fertility: thesis project for water-soil-plant model improvement
Beckers, Eléonore ULg; Degre, Aurore ULg

Poster (2008, January)

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic ... [more ▼]

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic conductivity…is often ignored. The present thesis project purposes the development of a physically-based model linking agricultural practices and changes on soil physical properties. [less ▲]

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See detailAgonistic sounds in the skunk clownfish (Amphiprion akallopisos) : spectral and temporal basis for fish size assessment and individual recognition
Colleye, Orphal ULg; Frederich, Bruno ULg; Vandewalle, Pierre ULg et al

Poster (2008)

Clownfishes (i.e. Amphiprion akallopisos) are territorial fishes that use sound production to defend their anemone territory. They live in social groups within which there is a size-based dominance ... [more ▼]

Clownfishes (i.e. Amphiprion akallopisos) are territorial fishes that use sound production to defend their anemone territory. They live in social groups within which there is a size-based dominance hierarchy. In such a system, agonistic interactions are usual and play an important role in social organization by maintaining size differences between individuals adjacent in rank. Sounds are known to be used in agonistic interactions; they could therefore be associated with aggressive display within the group hierarchy. In the present study, we compared the sonic characteristics between individuals of different sizes and of different sexual status (non-breeder, male and female). Sound analysis revealed that dominant frequency and pulse length are highly correlated with fish size, and are not related to sex. No information related to size can be extracted from the pulse period, interpulse duration and number of pulses per train. Evidence is provided that dominant frequency and pulse length are morphologically determined signals related to fish size. In that case, smaller individuals produce shorter duration and higher frequency sounds than larger individuals. Both sonic features are signals conveying information related to the size of the emitter. This finding could be of significant importance, and might help to understand one of the mechanisms that carry the clue for individual recognition within the group hierarchy. [less ▲]

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See detailDevelopment of Procedural Learning in Children : The “Toy Task”
Lejeune, Caroline ULg; Meulemans, Thierry ULg

Poster (2008)

The authors generally agree that procedural learning abilities play an important role in the child development and that they are present early in childhood. However, few studies have been carried out in ... [more ▼]

The authors generally agree that procedural learning abilities play an important role in the child development and that they are present early in childhood. However, few studies have been carried out in this area because of the difficulty to develop tests specifically designed for the evaluation of these capacities in young children. In this study, we presented a new task aimed to evaluate visuo-motor skill learning in the young child. The purpose of the present study was to explore whether procedural learning abilities are present in the same extent in 4-year as 7- and 10-year-old children. Our sample is composed of 60 children divided into 3 age-groups. The task is composed of 5 blocks of 12 trials during which each subject has to use a reversed mouse of a computer in order to “catch” different toys appearing on the screen; 2 blocks of 12 trials was administered after a 15 minutes delay. Our results show an important difference between the groups for the first block; on the other hand, for the next blocks, the results show an absence of age-related difference between participants in our perceptive-motor skill learning task, confirming the idea according to which procedural learning abilities are efficient early in development (the difference observed during the first block being probably due to the intervention of executive mechanisms). [less ▲]

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See detailDINEOF univariate reconstruction of missing satellite data from the North Sea Belcolour-1 database.
Sirjacobs, Damien ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2008)

The Belcolour-1 database holds more than 4 years of uniformly resampled MERIS chlorophyll (CHL), total suspended matter (TSM), MODIS-AQUA CHL, TSM and sea surface temperature (SST) over the North Sea. A ... [more ▼]

The Belcolour-1 database holds more than 4 years of uniformly resampled MERIS chlorophyll (CHL), total suspended matter (TSM), MODIS-AQUA CHL, TSM and sea surface temperature (SST) over the North Sea. A first step of the RECOLOUR* project consists in the univariate reconstruction of missing data with the DINEOF method (Data Interpolating Empirical Orthogonal Functions). In particular, the DINEOF treatment of MERIS CHL and TSM images available for the year 2003 allowed an efficient synthesis of the coherent modes of variability existing at the scale of the whole North Sea. For both parameters, 4 modes were retained by general cross validation as an optimum for the reconstruction of missing data. For CHL, the first spatial mode shows the high influence of coastal nutrients outputs (mainly continental estuaries and diffused coastal sources) and the lower concentration in the well stratified central and northern part of the North Sea compared to the southern bight and the eastern English Channel. The spatial trends described by the first mode are permanent features during the year, although slightly enhanced during the summer and reduced during winter. The second spatial mode correspond to the main algal blooming events (spring and autumn) with increased concentrations in the whole southern bight of the north sea, around the Isle of Wight and in frontal alike structure north-west from Denmark. The third Eofs describes well the dynamics of an early phytoplankton bloom occurring in march along the Norwegian coast, where a strong stratification induced by an output of cold water from Baltic Sea provides good light conditions to phytoplankton. Concerning TSM, the first spatial mode shows the dominant influence of large estuaries and of resuspension from shallow coastal sedimental plains. The patterns suggest a general transport of sediments from south-east England up to the northern Dutch coastal waters, as a clear distinction between the stratified northern part and the well mixed and charged southern and German bights. Although these trends are permanent during the year, the range of the spatial variations are slightly reduced during the summer, following the reduction of resuspension, of total sediment outputed by rivers and of advection along continental coasts. The second mode shows a clear seasonal signal. The winter influence of the second spatial mode can be understood as general sediment enrichment due to higher resuspension, but a clear influence of intense winter terrestrial water outflows leading to lower sediment concentration in the plumes then in the surrounding waters. This is clear for the Elbe river discharge, the whole natural part of the Wadden Sea and the Seine river plume. The Scheelde and Thames rather seems to be just neutralizing the seasonal TSM resuspension signal. The Rhine river discharge seems to make exception as no influence is detected in the second spatial mode. During summer, the contribution of the second EOF is reversed with a general reduction of suspended matter concentration in most part of the area but some local sediment enrichment at specific river discharges. Original MERIS CHL and TSM images were filled and reconstructions were produced at a daily interval based on a linear interpolation of the temporal modes. From this, weekly averages could be calculated at stations such as the turbidity maximum of the Scheelde river plume, showing the onset of the spring bloom co-occurring with a period characterised both by the TSM seasonal reduction and by important TSM temporal variability. [less ▲]

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See detailUse of the daily differencing approach to evaluate uncertainties affecting eddy covariance measurements
Laffineur, Quentin ULg; Aubinet, Marc ULg

Poster (2008)

The eddy covariance technique is recognised to be the most adapted micrometeorological method to study the exchange processes between terrestrial ecosystems and the atmosphere. Like all other methods, it ... [more ▼]

The eddy covariance technique is recognised to be the most adapted micrometeorological method to study the exchange processes between terrestrial ecosystems and the atmosphere. Like all other methods, it is submitted to systematic and random measurement errors. A thorough analysis of these errors is necessary in order to set the limits of validity of the method and to quantify the uncertainty that affects net carbon exchange computed with this method. In this presentation, we’ll concentrate on the random errors using the dailydifferencing approach (DDA) developed by Hollinger and Richardson (2005). The interest of this approach is that it requests only single tower measurements and is thus applicable to all flux tower sites. In this approach, uncertainties are estimated by comparing flux values taken at two successive days at the same hour and under similar meteorological conditions. The analysis may be applied to sensible heat, latent heat and CO2 flux densities. It was applied here to the eddy-covariance data from the Vielsalm mixed forest site (10 years of data) and from the Lonzée agricultural site (4 years of data). Both sites are situated in Belgium and are part of the Carboeurope IP network. The study is developed in the frame of the European IMECC project. The absolute and relative random error was quantified for both sites. Their daily evolution and their dependencies on different climate conditions (magnitude of the flux, PPFD, Rnet, wind velocity, wind direction, clarity index) were analysed. For both sites, the absolute random error increases linearly with the absolute value of flux. This is the principal factor controlling the random error. More particularly the response of the random error to wind velocity was analysed. For the CO2 flux, the absolute random error decreases with increasing wind speed. This effect is more important for the agricultural site than for the forest site. The behaviour of the relative random error with wind speed is more contrasted: it generally decreases with increasing wind speed at low velocities but, for some directions may increase with wind speed at high velocities. In addition, the random error was found very dependent on wind direction at the forest site probably as a result of site heterogeneity. [less ▲]

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See detailNew insights in auditory organ development: the inner pillar cell goes it own way
Thelen, Nicolas; Malgrange, B; Thiry, Marc ULg

Poster (2008)

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See detailDosage des pepsinogènes bovins A et C par RIA
Terroni, D.; Melo de Sousa, Noelita ULg; Michiels, J. A. et al

Poster (2008)

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See detailMechanisms of ATM regulation by TGF-beta
Paupert, Jenny ULg; Barcellos-Hoff, Mary-Helen

Poster (2008)

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See detailHeterogeneity of zooxanthellae density in the coral Acropora globiceps around Moorea (French Polynesia)
Ladrière, Ophélie ULg; van Lierde, Elodie; Penin, Lucie et al

Poster (2008)

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts ... [more ▼]

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts). Heterogeneity of coral bleaching at different observation scales (within a colony, among neighbouring colonies of the same species or on a wider scale) remains largely misunderstood. The present work explores intracolonial, spatial and temporal variations of zooxanthellae density in the coral Acropora globiceps Dana 1846, over a period of three months on the forereef of Moorea, French Polynesia. In our study, intracolonial zooxanthellae densities did not vary significantly. However, zooxanthellae densities differed slightly between inner and outer branches but this trend was not significant at 6, 12 and 18 meters depth. On a wider scale, zooxanthellae densities also vary spatially : a positive correlation was observed between depth and symbiont density (density increases when light intensity decreases, so according to depth). Moreover, the location of colonies exposed to different hydrodynamical conditions was not a parameter controling the concentration of zooxanthellae. Finally, the temporal variation of zooxanthellae densities did not show significant variations even if it decreased slightly over the study period. The results of this study highlight the importance of accounting for variations within and among colonies to determine zooxanthellae densities and to assess the evolution of zooxanthellae populations. [less ▲]

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See detailModel of Irrigation Development for Floodplain of the Tonle Sap Great Lake
someth, Paradis; Kubo, n; Ly, Sarann ULg et al

Poster (2008)

Rice is a staple crop in Cambodia. To meet the large requirement of rice consumption due to rapid increased population and to boost the economy, the rice production in this country needs to be enhanced ... [more ▼]

Rice is a staple crop in Cambodia. To meet the large requirement of rice consumption due to rapid increased population and to boost the economy, the rice production in this country needs to be enhanced. The Tonle Sap Great Lake of Cambodia has huge floodplain suitable for large amount for rice production and potential water resources for rice-based farming system. Due to large variation of water level of the lake, some areas around the lake are flooded in wet season and shortage of water in dry season. Studies to exploit the flood and the water rsources of the lake and its tributaries have not been well conducted yet. The West Baray and Batheay irrigation systems are located in the floodplain of the Tonle Sap Great Lake and the Mekong River. In this research, the irrigation systems are studied as model sites for future development of the floodplain of the Tonle Sap Great Lake. [less ▲]

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See detailRole of thiamine triphosphate and adenosine thiamine triphosphate in Escherichia coli
Gigliobianco, Tiziana ULg

Poster (2008)

Thiamine triphosphate (ThTP) is found in all organisms studied from bacteria to mammals. In E. coli, ThTP is accumulated as a result of amino acid starvation in the presence of a suitable carbon source ... [more ▼]

Thiamine triphosphate (ThTP) is found in all organisms studied from bacteria to mammals. In E. coli, ThTP is accumulated as a result of amino acid starvation in the presence of a suitable carbon source such as glucose, pyruvate and lactate. On the other hand, adenosine thiamine triphosphate (AThTP), that was recently discovered in E. coli, accumulates in the absence of a carbon source. While AThTP is synthesized in E. coli according to the reaction ThDP + ADP (ATP) ↔ AThTP + Pi (PPi) by a soluble high molecular mass nucleotidyl transferase, the mechanism of synthesis of ThTP remains unknown. Though both bacterial and mammalian adenylate kinases are able to catalyze ThTP synthesis at a very low rate in vitro, this reaction is not responsible for ThTP synthesis in vivo. AThTP is synthesized in E. coli incubated in minimal medium devoid of any carbon source. The addition of glucose leads to the immediate disappearance of AThTP and the appearance of ThTP. ThTP production is strongly impaired in the presence of protonophores, even when a high intracellular concentration of ATP is maintained through glycolysis. Our results suggest that ThTP accumulation requires a high protonmotive force rather than a high intracellular ATP concentration. On the other hand, AThTP is synthesized under conditions of limited energy availability, either in the absence of a carbon source or in the presence of a carbon source and metabolic inhibitors or uncouplers. Interestingly, ThTP and AThTP never accumulate simultaneously in high amounts. Our results suggest that in E. coli both compounds might therefore act as specific “alarmones”, responding to different conditions of cellular stress. [less ▲]

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See detailOptimization of a drug-cyclodextrin complexation reaction by a static supercritical carbon dioxide process
Brion, Michael; Nizet, Dominique; Evrard, Brigitte ULg

Poster (2008)

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See detailInter-annual variability of the carbon dioxide oceanic sink south of Tasmania
Borges, Alberto ULg; Tilbrook, B.; Metzl, N. et al

Poster (2008)

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See detailCesàrolite, a possible member of the birnessite group of minerals
Perchiazzi, Natale; Hatert, Frédéric ULg; Merlino, Stefano et al

Poster (2008)

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See detailEffects of light on cerebral activity
Vandewalle, Gilles ULg; Dijk, Derk-Jan; Phillips, Christophe ULg et al

Poster (2008)

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See detailE. coli HU dimers dynamics studied by NMR
Augustyniak, R.; Damblon, Christian ULg; Castaing, B.

Poster (2008)

HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 ... [more ▼]

HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 (α and β sharing 70% of sequence identity) and one heterodimer (αβ). Dimeric forms relative abundance varies during cell growth and in response to environmental changes, suggesting that each dimer plays different physiological roles. Unlike other HU proteins which melt through a single step (N2<=>2D), E. coli dimers melt according to a two-step mechanism (N2<=>I2<=>2D). The native dimer, N2, melts partially into a dimeric intermediate, I2, which in turn yields the unfolded monomers, D. Circular Dichroism studies indicate that the intermediate, I2, corresponds to an HU dimer having partly lost its a-helices. Here we compared dynamic properties and structural features of E. Coli HU dimers at different temperatures in order to determine the secondary strutural elements still present in the I2 intermediate. Isotopically labelled proteins (15N, 13C) have been used to perform backbone assignment for all dimeric forms using sequential triple-resonance experiments. 15N-HSQC at different temperatures (288-323K) were recorded .Intensity variations and chemical shift perturbations allow the identification of the secondary element lost in the intermediate I2 confirming the high dynamics of arms responsible for protein-DNA interactions and the thermal stability of HTH motif in all dimeric forms. [less ▲]

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See detailMechanisms of ATM regulation by TGF-beta
Paupert, Jenny ULg; Barcellos-Hoff, Mary-Helen

Poster (2008)

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See detailPotential implication of host/symbiont recognition mechanisms in coral bleaching
Vidal-Dupiol, Jérémie; Mitta, Guillaume; Roger, Emmanuel et al

Poster (2008)

Bleaching in corals can be attributed to loss of endosymbiotic zooxanthellae and/or loss of photosynthetic pigments within zooxanthellae. This major disturbance of the reef ecosystem is principally ... [more ▼]

Bleaching in corals can be attributed to loss of endosymbiotic zooxanthellae and/or loss of photosynthetic pigments within zooxanthellae. This major disturbance of the reef ecosystem is principally induced by increases in water temperature. Since the beginning of the 80ís and the onset of global climate change, this phenomenon has been occurring at increasing rates and scales, and with increasing severity. In this study, we focused on finding early regulated genes involved in bleaching. In aquaria, one set of Pocillopora damicornis nubbins was subjected to a gradual seawater temperature increase from 28°C to 32°C over 15 days, and a second control set remained at constant temperature (28°C). Bleaching was monitored by measuring zooxanthellae density. The mRNA differentially expressed between the stressed state (sampled just before the onset of bleaching) and the non stressed state (control) were isolated from the nubbins by Suppression Subtractive Hybridization. The corresponding cDNA were sequenced and confronted to sequence databases to obtain gene similarities. Finally, transcription rates of the most interesting genes were conducted by Q-PCR. Two particularly interesting candidate genes showed an important decrease in their transcription rates following thermal stress and before zooxanthellae loss. These two genes show similarities with genes involved in host/symbiont and host/parasite models. The implication of these molecular actors suggests a possible role of recognition mechanisms between the host and its symbiont, in the breakdown of the symbiosis during the bleaching phenomenon. Experiments such as RACE-PCR, in situ hybridization and immunohistochemistry are currently underway to confirm our hypotheses. [less ▲]

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See detailInfluence of nitrogen fertilizer treatments on soft wheat starch characteristics
Massaux, Carine; Sindic, Marianne ULg; Lenartz, Jonathan et al

Poster (2008)

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See detailRegulation of HIV late phase transcription by CTIP2
Cherrier, Thomas ULg; Rohr, Olivier

Poster (2008)

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See detailCompositional protein analysis of HDL by SELDI-TOF MS during experimental endotoxemia
Levels, Johannes HM; Marée, Raphaël ULg; Geurts, Pierre ULg et al

Poster (2008)

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See detailAphid – ant mutualism : How do aphids focus ant foraging ?
Verheggen, François ULg; Diez, Lise; Detrain, Claire et al

Poster (2008)

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See detailImpacts and uncertainties of upscaling of remote sensing data validation for a semi-arid woodland.
Hufkens, K; Bogaert, Jan ULg; Dong, Q H et al

Poster (2008)

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See detailFunctional properties of phospholipids and proteins from rapeseed as native tensioactives
Vaca Medina, Guadalupe; Mouloungui, Zéphirin; Deleu, Magali ULg et al

Poster (2008)

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See detailFra-1 and its associated transcriptome are central determinants of human breast cancer metastasis
Desmet, Christophe ULg; Prieur, A; Reyal, F et al

Poster (2008)

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See detailOperational monitoring of multi-qubit entanglement classes via tuning of local operations
Bastin, Thierry ULg; Thiel, C.; von Zanthier, J. et al

Poster (2008)

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See detailContrastief taalonderzoek geïntegreerd aanpakken: hoe en waarom?
Rasier, Laurent ULg; Hiligsmann, Philippe; Baelen, Mélanie et al

Poster (2008)

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See detailOperational monitoring of multi-qubit entanglement classes with trapped ions
Bastin, Thierry ULg; Thiel, C.; von Zanthier, J. et al

Poster (2008)

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See detailMechanism of sound production in Oreochromis niloticus
longrie, Nicolas; van Wassemberg, Sam; Mauguit, Quentin et al

Poster (2008)

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See detailSwitching entanglement classes via local operations
Bastin, Thierry ULg; Thiel, C.; von Zanthier, J. et al

Poster (2008)

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See detailProtein synthesis and folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2008)

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See detailProgress towards new atomic species laser cooling
Krins, Stéphanie ULg; Saint-Georges, P.; Bastin, Thierry ULg

Poster (2008)

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See detailDecreased Thymosin Beta 4 Expression Results in Poor Prognosis and Decreased Survival in Multiple Myeloma
CAERS, Jo ULg; Hose, Dirk; Kuipers, Ine et al

Poster (2008)

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See detailTowards an iron magneto-optical trap
Krins, Stéphanie ULg; Saint-Georges, P.; Bastin, Thierry ULg

Poster (2008)

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See detailThe TNF receptor signalling network: Modular modelling and cell-type specific analysis
Waldherr, S; Doszczak, M; Schliemann, Monica ULg et al

Poster (2008)

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See detailGeneration of symmetric Dicke states
Thiel, C.; von Zanthier, J.; Bastin, Thierry ULg et al

Poster (2008)

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See detailPhosphorylation of huntingtin by cyclin dependent kinase 5.
Godin, Juliette ULg; Anne, Sandrine; Humbert, Sandrine

Poster (2008)

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See detailProtein synthesis and folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2008)

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See detailLaser cooling of new atomic species - scheme of operation
Saint-Georges, P.; Krins, Stéphanie ULg; Bastin, Thierry ULg

Poster (2008)

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See detailHETEROGENEITY OF ZOOXANTHELLAE DENSITY IN THE CORAL ACROPORA GLOBICEPS AROUND MOOREA (FRENCH POLYNESIA)
Ladrière, Ophélie ULg; van Lierde, Elodie; Penin, Lucie et al

Poster (2008)

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts ... [more ▼]

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts). Heterogeneity of coral bleaching at different observation scales (within a colony, among neighbouring colonies of the same species or on a wider scale) remains largely misunderstood. The present work explores intracolonial, spatial and temporal variations of zooxanthellae density in the coral Acropora globiceps Dana 1846, over a period of three months on the forereef of Moorea, French Polynesia. In our study, intracolonial zooxanthellae densities did not vary significantly. However, zooxanthellae densities differed slightly between inner and outer branches but this trend was not significant at 6, 12 and 18 meters depth. On a wider scale, zooxanthellae densities also vary spatially : a positive correlation was observed between depth and symbiont density (density increases when light intensity decreases, so according to depth). Moreover, the location of colonies exposed to different hydrodynamical conditions was not a parameter controling the concentration of zooxanthellae. Finally, the temporal variation of zooxanthellae densities did not show significant variations even if it decreased slightly over the study period. The results of this study highlight the importance of accounting for variations within and among colonies to determine zooxanthellae densities and to assess the evolution of zooxanthellae populations. [less ▲]

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See detailInteraction-free Generation of Angular Momentum Eigenstates in Remote Qubits
Thiel, C.; Maser, A.; Bastin, Thierry ULg et al

Poster (2008)

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See detailSox10 is not necessary for auditory neurons survival
Breuskin, I; Bodson, M; Thelen, Nicolas ULg et al

Poster (2008)

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See detailA Critical Study of Primate Reintroduction Projects in the Mesoamerican Region.
Martin, Sarah ULg; Huynen, Marie-Claude ULg; Libois, Roland ULg

Poster (2008)

Very little is known about primate reintroduction projects in Mesoamerica. The aim of this study was to focus on existing projects concerning primate re-introductions, conservation-introduction ... [more ▼]

Very little is known about primate reintroduction projects in Mesoamerica. The aim of this study was to focus on existing projects concerning primate re-introductions, conservation-introduction, translocation, as well as on reinforcement-supplementation of parallel initiatives. In order to create a database, a survey was sent to a panel of scientists in Mexico, Belize, Guatemala, Honduras, El Salvador, Nicaragua, Costa Rica and Panama, and we visited five projects in Costa Rica from March to August 2006. A total of 19 projects were found: Costa Rica hosted the higher number (7) while Salvador and Nicaragua did not host any. Fourteen out of those 19 projects concerned confiscated animals. Six of the 7 Costa Rican projects used confiscated primates; the other ones came from wild populations or were born in captivity. Alouatta palliata and Ateles geoffroyi are the most common species subject to reintroduction effort in the Mesoamerican region. A wide range of factors seem to influence reintroduction success such as: the project’s budget, the release site, the mode of transportation, the caging setting, the support from local communities, the presence of environmental education programs, but also the age of primates, etc. Each project seems to work as a completely separated entity, with its own definition of reintroduction success and its own policy. There’s no communication between the projects, no matter if they are based in the same country or work on the same species. A reintroduction guideline for this region should be established as well as a regional network coordinating the information. [less ▲]

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See detailPhospholipids quantification in vegetable oil degumming residues by HPLC-ELSD
Pierart, Céline ULg; Danthine, Sabine ULg; Wathelet, B et al

Poster (2008)

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See detailHistology and ultrastructure of the elastic spring apparatus in Synodontis eupterus
Fabri, Grégory; Montchowui, E; Vandewalle, Pierre ULg et al

Poster (2008)

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See detailOptimisation of blocked designs in fMRI studies
Maus, Bärbel ULg; van Breukelen, G. J. P.; Goebel, R. et al

Poster (2008)

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See detailThe effects of cognitive remediation therapy on AM deficits in schizophrenia patients
Boulanger, Marie ULg; Collet, Denis; Cornet, Lidvine et al

Poster (2008)

Schizophrenia is accompanied by deficit in autobiographical memories (AM). These impairments are correlated to difficulties in imagining specific events that might happen to them in the future ... [more ▼]

Schizophrenia is accompanied by deficit in autobiographical memories (AM). These impairments are correlated to difficulties in imagining specific events that might happen to them in the future (D’Argembeau, Raffard et Van-der-Linden, 2008). Previous study suggested that the specific autobiographical memory (past and future) may be improved by an AM remediation therapy (Blairy et al., accepted). The aim of the present study was to compare performances from individuals involved in an AM remediation intervention to individuals involved in the cognitive remediation therapy program from Brenner (IPT) (Pomini, Neis, Brenner, Hodel et Roder, 1998). The results showed that the participants tend to recall more specific events after therapies, confounded pass and future events (t(7) = 3.28 ; p = .013). These results would be promising for future research. [less ▲]

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See detailBioethanol subproducts as a basis of plant biorefinery
Destain, Jacqueline ULg; Laurent, Pascal ULg; Ndoye, Bassirou et al

Poster (2008)

The philosophy of our research is built on the behaviour of non-sugar compounds from biomass during ethanolic fermentation. Saccharomyces cerevisiae, industrially used to bioconvert sugar into ethanol ... [more ▼]

The philosophy of our research is built on the behaviour of non-sugar compounds from biomass during ethanolic fermentation. Saccharomyces cerevisiae, industrially used to bioconvert sugar into ethanol under anaerobic conditions, assimilates some compounds present in the fermentable juice (proteins, amino acids,...). But others compounds are not used by the yeast. They leave in the vinasse after ethanol distillation and are thus concentrated by the process. Our purpose is to discuss the behaviour of betaine, saponins and raffinose in sugar juices (raw juice, thin juice, thick juice) and in vinasse after ethanolic fermentation. An attention will be given to some high added value products (like betaine) and the potentialities to use fermentation as a tool of purification in white chemistry. [less ▲]

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See detailSecreted subtilisins of Microsporum canis are involved in adhesion of arthroconidia to feline corneocytes
Mathy, Anne ULg; Tabart, Jérémy; Mignon, Bernard ULg et al

Poster (2008)

Microsporum canis is a pathogenic fungus that causes a superficial skin infection called dermatophytosis mainly in cats, dogs and humans. Like other dermatophytoses, the physiopathology of this dermatosis ... [more ▼]

Microsporum canis is a pathogenic fungus that causes a superficial skin infection called dermatophytosis mainly in cats, dogs and humans. Like other dermatophytoses, the physiopathology of this dermatosis remains largely unknown. From a fungal perspective, the infection process can be divided in three steps: adhesion of M. canis arthroconidia to corneocytes, conidial germination, and fungal invasion of the keratin network. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. However, several previously characterized secreted fungal endoproteases like subtilisins (Sub), including the keratinolytic protease Sub3, are secreted in vivo and could be involved in the first pathogenic steps. The objective of this study were (1) to develop an in vitro model to study M. canis adherence to feline corneocytes and (2) to assess whether the Sub are involved in fungal adhesion. An arthroconidial suspension was spread over the surface of reconstituted feline epidermis (RFE). Co-cultures were incubated for varying lengths of time and adherent conidia were labelled using Calcofluor white and counted. In subsequent assays arthroconidia were exposed to the serine protease inhibitor chymostatin or a mixture of two anti-Sub3 monoclonal antibodies (Mabs) one hour prior to the adherence assay. In our model, adherence of M. canis arthroconidia to RFE is time-dependent, beginning within two hours and still increasing after six hours. Chymostatin and Mabs inhibit M. canis adherence to RFE by 53 and 23 % respectively, which suggests that subtilisins and particularly Sub3, are fungal virulence factors involved in the adherence process. [less ▲]

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