References of "Poster"
     in
Bookmark and Share    
Peer Reviewed
See detailPoster session 6: Saturday 6 December 2014, 08:30-12:30Location: Poster area.
Henri, C.; DULGHERU, Raluca Elena ULg; Magne, J. et al

Poster (2014, December)

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailRecherche de sensations, sexualité et pornographie à l’adolescence
Puglia, Rosa ULg; Glowacz, Fabienne ULg

Poster (2014, December)

Introduction : L’adolescence est une période tumultueuse caractérisée par des transformations d’ordre physique, psychologique, cognitive et sociale ainsi que par des conduites exploratoires et de ... [more ▼]

Introduction : L’adolescence est une période tumultueuse caractérisée par des transformations d’ordre physique, psychologique, cognitive et sociale ainsi que par des conduites exploratoires et de recherche de sensations dans lesquelles la consommation de pornographie peut prendre part. L’exposition à la pornographie durant l’adolescence peut s’inscrire dans ce processus développemental avec le risque d’influencer les conduites sexuelles du jeune. Cette thématique peut, dès lors, susciter de nombreuses questions. Méthodologie : Notre recherche, réalisée sur une population d’adolescents âgé entre 15 et 19 ans (N=319), vise à analyser les conduites de consommation de pornographie (motivations, représentations et comportements) en lien avec la recherche de sensations, une dimension prégnante à l’adolescence. Plus particulièrement, nous avons analysé les motivations de ces jeunes à visionner des images et films à caractère pornographique et ce qui les amène à arrêter. Nous nous sommes, également, interrogés quant à l’influence de la consommation pornographique au niveau du développement psycho-sexuel et des pratiques sexuelles de ces jeunes. Pour ce faire, nous avons administré à ces jeunes un questionnaire comprenant, en plus des items se rapportant à la sexualité et la pornographie, plusieurs tests évaluant des dimensions du fonctionnement psychologique et social (test de solitude, recherche de sensation, relations aux pairs, délinquance auto-révélée,... ). Résultats : Nos résultats indiquent qu’une majorité des adolescents ont déjà visionné de la pornographie. L’intérêt pour ce média ainsi que son usage augmente avec l’âge, atteint son apogée vers 16-17 ans puis diminue. Cette tendance curvilinéaire se retrouve également dans les conduites exploratoires telles que les conduites déviantes et la recherche de sensations, ce qui nous amène à envisager une désistance spontanée de la consommation de pornographie en fin d’adolescence. Conclusions : Ces résultats montrent l’importance de comprendre la consommation de pornographie dans un processus développemental et nous amène à envisager une désistance spontanée de cette conduite en fin d’adolescence. [less ▲]

Detailed reference viewed: 38 (4 ULg)
See detailPerceptions culturelles dans un contexte de néoplasme pédiatrique: Considérations transculturelles entre l’Occident et le Japon
Vander Haegen, Marie ULg; Roskam, Isabelle

Poster (2014, November 29)

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et ... [more ▼]

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et moraux sur base des repères culturels alloués depuis des décennies. Investiguer les balises culturelles et l’appréhension sociétale du néoplasme pédiatrique reste peu développé dans la littérature. Ce champ « anthropo-clinique » prend initialement son essor dans la clinique oncologique adulte où des résultats significatifs montrent un lien entre la culture et la perception du cancer. OBJECTIFS Les objectifs de cette étude sont multiples: (a) étudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant, (b) analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale, et (c) mettre en exergue les diverses implications cliniques pour les professionnels de la santé. METHODE Une revue de littérature est conduite afin de dégager les tendances culturelles dans la politique sanitaire, le service hospitalier, la relation médecin-malade, le concept de douleur, l’environnement familial et la perception de la mort au Japon et en Occident. Le choix s’est porté sur ce pays asiatique par la présence de certains repères culturels intéressants dans le domaine de l’oncologie pédiatrique. RESULTATS (a) Etudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant Les correspondances effectuées ont permis d’entrevoir la situation onco-pédiatrique comme relevant de faits biologiques et de faits culturels. Les paramètres culturels interviennent au sein des nombreuses conceptions et représentations sociales de la maladie cancéreuse d’un enfant. L’ensemble de ces perceptions sociales étant notamment influencées par les valeurs et les truismes véhiculés dans la culture. (b) Analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale. Eléments de similitudes : Il a été noté l’universalité des mécanismes pathogéniques du cancer chez l’enfant et le développement de politiques sanitaires dans la lutte contre le cancer. Eléments de dissimilitudes : A. Le Japon Le système familial japonais et son organisation des soins en cancérologie de l’enfant varient et se teintent de nombreuses coutumes shintoïstes et bouddhistes. Il a été identifié le chaos régnant dans ses familles où des attitudes désengagées existent. L’organisation hospitalière japonaise est décrite comme carencée en réseaux de soutien psychologique pour la mère, très souvent seule face à la maladie de son enfant. Des risques majeurs de dépression, de traits anxieux et d’un stress post-traumatique sont observés. Le médecin japonais dispose d’un pouvoir de décision médicale et sociale, se manifestant notamment par un degré de variabilité dans la communication diagnostique et pronostique envers la famille et l’école d’origine. La situation de soin est variable avec l’inclusion en premier lieu d’une pharmacopée naturelle afin de traiter le cancer et ses effets iatrogènes. Enfin, il subsiste une inhibition du partage social des émotions où, des attitudes de retenue et de silence chez les parents se manifestent durant tout le processus thérapeutique mais aussi en cas de décès de l’enfant. B. L’Occident Les pays occidentaux présentent une appréhension sociale et médicale singulière du cancer pédiatrique. La variabilité observée concerne l’organisation hospitalière et la communication médicale. Le praticien occidental établit une communication médicale ouverte avec l’enfant et sa famille et ce, dès le départ du processus thérapeutique. Le contexte curatif est caractérisé par une forte médication d’opioïdes afin de pallier aux nombreuses douleurs et effets des traitements. L’aménagement hospitalier permet la création de réseaux de soutien psychologique pour l’enfant et sa famille et l’établissement d’activités ludiques.(c) Mettre en exergue les diverses implications cliniques pour les professionnels de la santé L’intention de cette étude est de sensibiliser les professionnels de la santé à la pléiade de facteurs psycho-sociaux intervenant dans un contexte de maladie néoplasique. Éléments qui doivent requérir d’une bienveillance clinique où tant l’enfant traité que son entourage familial, doivent être entendus dans toute leur spécificité d’être humain. DISCUSSION Une stratégie d’action intéressante concernerait une grille de lecture culturelle du patient et de sa famille. Cette grille peut offrir un entendement de la diversité culturelle en psycho-oncologie et peut permettre au professionnel de réajuster finement son travail clinique dans un contexte quotidien de multiculturalité. Cette grille de lecture doit rester simple et proposer un petit nombre de « repères culturels » (items culturels) pour professionnels, leurs permettant d’ajuster certains paramètres cliniques (communication médicale, gestion des émotions, rapport à la mort, etc.). Cette considération ethnologique du patient doit supposer au préalable une formation de compétences dans le domaine de la culture et de la santé/maladie. [less ▲]

Detailed reference viewed: 53 (3 ULg)
Full Text
Peer Reviewed
See detailDélires et Hallucinations : Echelle transdiagnostique d’auto-évaluation.
Beaupain, Margaux; Thirion, Julie; Wagener, Aurélie ULg et al

Poster (2014, November 29)

Introduction : Les délires et les hallucinations sont des symptômes majeurs présents chez 80% des personnes souffrant d’un trouble psychotique (e.g., bipolaire, schizophrène). Cependant, ces phénomènes ... [more ▼]

Introduction : Les délires et les hallucinations sont des symptômes majeurs présents chez 80% des personnes souffrant d’un trouble psychotique (e.g., bipolaire, schizophrène). Cependant, ces phénomènes sont également présents dans d’autres troubles psychiatriques (e.g., troubles anxieux, dépression) et chez 8% des individus tout venants (Linscott & Van Os, 2012) suggérant l’importance de les évaluer. D’après la littérature, les populations cliniques et non-cliniques se distinguent d’avantage à travers différentes dimensions telles que la détresse, la conviction et le contenu que par la fréquence (Lincoln, 2007). Cependant, les évaluations existantes se centrent principalement sur la fréquence des phénomènes, ce qui laisse beaucoup d’informations cliniques de côté. De plus, elles ne sont que rarement basées sur une approche en continuum permettant d’appréhender la complexité et l’hétérogénéité des phénomènes (allant des perceptions aberrantes jusqu’aux délires et hallucinations). Enfin, ce sont souvent des hétéroévaluations qui sont chronophages et requièrent du personnel qualifié alors que certaines études suggèrent une bonne validité des autoévaluations de ces phénomènes (Se Hyun et al., 2010). Afin de pallier ces limites, nous avons développé une échelle transdiagnostique d’auto-évaluation des idées délirantes et hallucinations en se basant sur une approche en continuum. Méthode : Une revue de la littérature sur les caractéristiques et outils d’évaluations des idées délirantes et des hallucinations a été réalisée. Vingt-quatre items parmi les plus discriminants ont été sélectionnés et adaptés. Pour chaque item, huit dimensions ont été sélectionnées : fréquence, détresse, interférence, conviction, état de conscience, contenu positif VS négatif, agréabilité et attribution interne VS externe. Les individus répondent d’abord à la fréquence sur une échelle de type Likert en 5 points (« Oui, ça m’arrive souvent » à « Non, ça ne m’est jamais arrivé »). Si la réponse est positive, les sept autres dimensions sont évaluées via une échelle visuelle analogique de 0 à 10. Perspectives: L’échelle est actuellement en cours de validation. Elle sera pré-testée auprès d’étudiants avant d’être validée auprès de populations cliniques et non-cliniques. A long terme, cette échelle transdiagnostique d’auto-évaluation pourrait être utilisée en clinique comme un outil favorisant une meilleure prise en charge de ces symptômes et comme un nouvel outil de recherche. [less ▲]

Detailed reference viewed: 45 (9 ULg)
Full Text
See detailTracking stressed blood volume during vascular filling experiments
Pironet, Antoine ULg; Dauby, Pierre ULg; Chase, J. Geoffrey et al

Poster (2014, November 28)

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac ... [more ▼]

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac output after fluid infusion. [less ▲]

Detailed reference viewed: 20 (4 ULg)
Full Text
See detailFunctional analysis of FRD3 in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Motte, Patrick ULg et al

Poster (2014, November 28)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

Detailed reference viewed: 44 (13 ULg)
Full Text
See detailEstimating Ventricular Stroke Work from Aortic Pressure Waveform
Kamoi, Shun; Pretty, Christopher; Chiew, Yeong Shiong et al

Poster (2014, November 28)

Detailed reference viewed: 21 (2 ULg)
Full Text
See detailRole of the polyQ length and non-polyQ regions during the aggregation process into amyloid fibrils of model polyQ proteins
Huynen, Céline ULg; Willet, Nicolas ULg; Buell, Alexander K et al

Poster (2014, November 26)

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated ... [more ▼]

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated proteins. This polyQ expansion is thought to be the major determinant in the development of neurotoxicity, triggering protein aggregation into amyloid fibrils. A large body of evidence however suggests that non-polyQ regions modulate the aggregation process triggered by polyQ expansions. The interplay between the polyQ tract and non-polyQ regions is complex and still not fully understood. In order to better understand it, we previously designed and characterized model polyQ proteins made of the beta-lactamase BlaP with 23, 30, 55 and 79Q inserted at position 197 or 216. Our first results had indicated that our model is relevant to study polyQ aggregation since it recapitulates the aggregation properties of polyQ disease-associated proteins: there is a Q-threshold for the spontaneous formation of amyloid fibrils in solution, and above the threshold, the longer the polyQ, the faster the aggregation. Moreover, the structure of BlaP and the position of insertion of the polyQ tract influence their aggregation properties in solution. This work aims to better understand, at the molecular level, (i) the precise role of the polyQ length (23, 30, 55, 61, 67, 73 and 79Q), (ii) the conformation of the host protein (native or unfolded BlaP), (iii) the location of the polyQ tract within BlaP (197 or 216), (iv) the flexibility of the polyQ flanking sequences, and (v) the origin of constraints applied by BlaP to the inserted polyQ tract (at its N- or C-terminal end) on the structural, thermodynamic and aggregation properties of BlaP-polyQ chimeras, using a wide range of biophysical techniques (e.g., spectroscopy methods, quartz crystal microbalance, atomic force microscopy and dynamic light scattering). The effect on the aggregation properties will be determined on the spontaneous aggregation into amyloid fibrils in solution, and on the nucleation and on the elongation steps of amyloid fibril formation. For this purpose, new chimeras containing 61, 67 and 73Q at position 197, or 55Q inserted at position 197 in between two different protease’s cleavage sites, that are relatively flexible, will be moreover created. Our results first demonstrate that the spontaneous aggregation into amyloid fibrils in solution is correlated to the polyQ length with an exponential growth function, and that the elongation rate is linearly correlated to the polyQ length, independently of the protein context (i.e., conformation of BlaP, and/or location of the polyQ tract, and/or polyQ peptides inserted or not within BlaP). However, the location of the polyQ tract inside BlaP, and/or its conformational state, and/or the flexibility of polyQ flanking sequences, and/or the origin of constraints applied to the polyQ tract drastically influence the ability of a polyQ tract to trigger the nucleation and/or the elongation step of amyloid fibrils (variation in the Q-threshold and in the absolute rate of both steps). Altogether, our results suggest that non-polyQ regions constitute an additional potential therapeutic target, more specific than drugs targeting the polyQ sequence, to interfere with the nucleation and/or the elongation of amyloid fibrils, associated to neurotoxicity. A possible drug could be constituted by a ligand specific to non-polyQ regions of disease-associated proteins, which further increases the constraints applied to the polyQ expansion to prevent the disease onset and/or progression. [less ▲]

Detailed reference viewed: 22 (4 ULg)
Full Text
See detailUse of 13C-NMR in structural elucidation of polysaccharides: case of locust bean gum
Gillet, Sébastien ULg; Aguedo, Mario ULg; Blecker, Christophe ULg et al

Poster (2014, November 25)

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic ... [more ▼]

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic structure is the same for all galactomannans (Fig. 2). However, when locust bean gum is extracted at different temperatures, the generated fractions exhibit different properties in aqueous solution (viscosity, viscoelasticity, gel formation, thermohydrolysis resistance, etc.). This means that there are differences within the fine structure of the polymers (although the basic structure is the same). Analysis of [13C]-NMR spectra of galactomannans, in combination with other techniques, can provide capital information about fine structural elucidation of the polymers. The method specifies the distribution of lateral galactosyls along the main chain of mannans. Two fractions extracted from locust bean gum at 25 and 80 °C (respectively GM25 and GM80) were comparatively studied by [13C]-NMR. Mannosyls/Galactosyls (M/G) ratios can be determined by considering the intensities of C-1 mannose and galactose signals in [13C]-NMR spectra. This method provides results relatively close to those obtained by GC-MS analysis. Spectra also showed that resonance from C4 of D-mannose residues were split, in evident dependence upon the nearest-neighbor probabilities (“diad frequencies”) of D-galactosyl groups along the mannan chains (Fig. 2). Diad frequencies were obtained by integrating C4(Man) peak areas. F11, F21/F12 and F22 gave respectively the di-, mono- or non-substituted mannose pairs proportions. High percentages of F11 and F22 therefore indicate a more non-homogeneous distribution of lateral galactosyls along the polysaccharide backbone as observed for GM80. The percentages of total lateral substituents obtained by C4(Man) peak analysis [F11 + (F21 or F12)/2] were fairly well correlated with M/G ratios. Splitting of the C-6 substituted D-mannose resonance provides, therefore the basis for determining the next-nearest-neighbor probabilities (triad frequencies) (Fig. 2). However, the spectrum is often not sufficiently resolved to accurately quantify and interpret the results. [less ▲]

Detailed reference viewed: 24 (7 ULg)
Full Text
See detailImpact of steam explosion treatment on chemical configuration of Tall Fescue lignin : structural elucidation using NMR spectroscopy
Maniet, Guillaume ULg; Jacquet, Nicolas ULg; Gillet, Sébastien ULg et al

Poster (2014, November 24)

In the economic and energy context of our society, it is universally recognized that alternatives to petrochemicals products must be found. To overcome this problem, renewable lignocellulosic biomass ... [more ▼]

In the economic and energy context of our society, it is universally recognized that alternatives to petrochemicals products must be found. To overcome this problem, renewable lignocellulosic biomass could be used to produce high value products. To achieve this objective, pretreatment processes are required to allow the breakdown of lignocellulosic structure and increase accessibility of the material. In this way, steam explosion is a thermo-mechano-chemical pretreatment which allows the opening of lignocellulosic material structural components and includes modifications of the physical properties of the material, hydrolysis of hemicellulosic components and modification of the chemical structure of lignin [1]. This study is focused on the impact of various steam explosion treatments on the chemical configuration of tall fescue lignin. NMR analyses perform on the Festuca L. pretreated samples show variations of links with treatment intensity. Observations show double phenomen :re-polymerization and depolymerization of the lignin structure during steam explosion process [2]. In parallel, HPSEC analyses show modifications in the molecular weight of the lignin obtained after the steam explosion treatment. [less ▲]

Detailed reference viewed: 17 (8 ULg)
Full Text
See detailIn vitro study toward the endocrine activity and the genotoxic potential of migration products from plastic baby bottles
Simon, Coraline ULg; Onghena, M.; Covaci, A. et al

Poster (2014, November 20)

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, widely used in a variety of common products including digital media (e.g ... [more ▼]

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, widely used in a variety of common products including digital media (e.g., CDs, DVDs), electrical and electronic equipment, automobiles, sports safety equipment, reusable food and drink containers , as well as baby bottle. During the last decades, in several studies, the migration of BPA is documented to be a well-known source of food contamination. Some studies have shown that BPA, which is able to mimic the action of and hormone and to disrupt normal endocrine function, may be associated to several health problems and diseases. Recently, the European Union took a series of measures, including a ban for the import and sale PC baby bottles to reduce BPA exposure to infants. Plastic alternatives to polycarbonate have massively appeared on Belgium market. Although there are several studies on BPA migration from polycarbonate into foodstuff under a variety of conditions, there is a small amount of information about consequences on human health of the potential exposure to chemicals migrating from PC alternatives, including bottles commonly labelled “free BPA”. In a recent opinion (No. 8697, 11.03.2010), the Belgium Superior Health Council's issued its concern regarding the currently used alternatives to PC. Furthermore, they asked to investigate the possible risks associated with the use of these alternatives. In order to evaluate the safety of these alternatives, the genotoxicity and the activity on several receptors of chemical compounds migrating from PC alternatives, identified by Simoneau & al, 2012 , were evaluated using reporter gene assays. Receptor agonistic and antagonistic activities of 39 pure compounds were measured. After the first screening, certain substances clearly showed an activity on several receptors such as BPA, and Bisphenol S, while only a few substances showed no reaction on the different receptor. None of the 39 components was genotoxic as identified in the Vitotox test. However, further experiments will be performed to characterize their activity and confirm the result for the genotoxicity. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Peer Reviewed
See detailMultiqubit symmetric states with maximally mixed one-qubit reductions
Baguette, Dorian ULg; Bastin, Thierry ULg; Martin, John ULg

Poster (2014, November 18)

We present a comprehensive study on the remarquable properties shared by maximally entangled symmetric states of arbitrary numbers of qubits in the sense of the maximal mixedness of the one-qubit reduced ... [more ▼]

We present a comprehensive study on the remarquable properties shared by maximally entangled symmetric states of arbitrary numbers of qubits in the sense of the maximal mixedness of the one-qubit reduced density operator. Such states are of great interest in quantum information as they maximize several measures of entanglement, such as Meyer-Wallach entropy [1] and any entanglement monotone based on linear homogenous positive functions of pure state within their SLOCC classes of states [2, 3]. When they exist, they are unique up to local unitaries within their SLOCC classes [3, 4]. They play a specific role in the determination of the local unitary equivalence of multiqubit states [5]. Moreover, they are maximally fragile (in the sense that they are the states which are the most sensitive to noise) and have therefore been proposed as ideal candidates for ultrasensitive sensors [6]. They appear in the litterature under various names : maximally entangled states [6], 1-uniform states [7], normal forms [3, 4] and nongeneric states [5]. We present a general criterion to easily identify whether given symmetric states are maximally entangled or not [9]. We show that these maximally entangled symmetric (MES) states are the only symmetric states for which the expectation value of the associated collective spin S of the system vanishes, which coincides with the definition of anticoherence to order one of spin states. This definition also coincides with the cancellation of the dipole moment of the Husimi function of the state. We then generalize these properties and show that a state is anticoherent to order t, <(S.n)^k> is independent of n for k = 1, . . . , t, where n is a unit vector, iff it has maximally mixed t-qubit reductions or iff all moments up to order 2t of its Husimi function vanish. We also establish the equivalence between anticoherent states to order t and unpolarized light states to order t [8], thereby encompassing various state characterizations under the same banner [9, 10]. We provide a nonexistence criterion allowing us to know immediately whether SLOCC classes of symmetric states can contain MES states or not. We show in particular that the symmetric Dicke state SLOCC classes never contain such MES states, with the only exception of the balanced Dicke state class for even numbers of qubits. We analyze the 4-qubit system exhaustively and identify and characterize all MES states of this system as well as the only 4-qubit state anticoherent to order 2. Finally, we analyze the entanglement content of MES states with respect to the geometric [11] and barycentric [12] measures of entanglement. [1] D. A. Meyer and N. R. Wallach, J. Math. Phys. 43, 4273 (2002). [2] Classes of states equivalent through stochastic local operations with classical communication. [3] F. Verstraete, J. Dehaene, and B. De Moor, Phys. Rev. A 68, 012103 (2003). [4] G. Gour and N. Wallach, N. J. Phys. 13, 073013 (2011). [5] B. Kraus, Phys. Rev. Lett. 104, 020504 (2010). [6] N. Gisin and H. Bechmann-Pasquinucci, Phys. Lett. A 246, 1 (1998). [7] A. J. Scott, Phys. Rev. A 69, 052330 (2004). [8] L. L. Sánchez-Soto, A. B. Klimov, P. de la Hoz, and G. Leuchs J. Phys. B : At. Mol. Opt. Phys. 46, 104011 (2013). [9] D. Baguette, T. Bastin, and J. Martin, Phys. Rev. A 90, 032314 (2014). [10] O. Giraud, D. Braun, D. Baguette, T. Bastin, and J. Martin, arXiv :1409.1106. [11] T.-C. Wei and P. M. Goldbart, Phys. Rev. A 68, 042307 (2003). [12] W. Ganczarek, M. Kus, and K. Zyczkowski, Phys. Rev. A 85, 032314 (2012). [less ▲]

Detailed reference viewed: 14 (3 ULg)
Full Text
See detailPRESPHOTO – a project to improve the preservation of cyanobacteria and diatom cultures
Deprez, Karolien; Vanormelingen, Pieter; Crahay, Charlotte ULg et al

Poster (2014, November 18)

The availability of biological material of guaranteed identity and quality in Biological Resource Centers is considered fundamental for scientific research and R&D, but depends heavily on adequate ... [more ▼]

The availability of biological material of guaranteed identity and quality in Biological Resource Centers is considered fundamental for scientific research and R&D, but depends heavily on adequate preservation methods. We present a new BRAIN-BE project on improving the preservation of two groups of photosynthetic microorganisms, cyanobacteria and diatoms, in two collections of the Belgian Co-ordinated Collections of Micro-organisms (BCCM). First, we will improve the cultivation success of diatoms from different habitats by testing different culture media. For organisms that keep resisting cultivation, we will develop DNA extraction and amplification, as well as morphological investigation based on single cells. Secondly, we will design and validate improved cryopreservation protocols for both diatoms and cyanobacteria, since cryopreservation is now the preferred method for the long-term storage of microalgal cultures. For that, the two-step cryopreservation method will be tested using several cryoprotectants and strains in different growth stages. Moreover, this will be compared with the encapsulation/dehydration method. For cyanobacteria, viability tests will be performed with vital dyes whereas diatoms’ survival will be assessed by PAM fluorometry. In addition, genome resequencing will be applied to determine the impact of the cryopreservation protocol(s) on genomic stability. Finally, a genomic DNA bank will be constructed and validated. This is highly complementary to preservation as living strains, given that some users require only genomic DNA and it may not be feasible to preserve the global microalgal species diversity as living cultures. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailGNSS observational bias in the frame of ionospheric studies
Wautelet, Gilles ULg; Warnant, René ULg

Poster (2014, November 17)

Detailed reference viewed: 23 (7 ULg)
See detailGlutamate controls brain estrogen synthesis during sexual interactions
de Bournonville, Catherine ULg; Aourz, Najat; Van Eeckhaut, Ann et al

Poster (2014, November 17)

Besides their long-lasting effects mediated by a modulation of gene transcription, brain-derived estrogens can rapidly regulate (within minutes) reproductive behaviors. In vitro, the activity of aromatase ... [more ▼]

Besides their long-lasting effects mediated by a modulation of gene transcription, brain-derived estrogens can rapidly regulate (within minutes) reproductive behaviors. In vitro, the activity of aromatase (AA), the enzyme responsible for the conversion of androgens into estrogens, is also regulated on a similar short time-scale, via phosphorylation of the enzyme resulting from changes in neuronal activity or glutamate release. Acute changes in AA have been documented ex vivo in specific brain regions following exposure to social or stressful stimuli but the mechanism underlying these regulations is not known. To investigate whether glutamate is implicated in these rapid changes in AA, male quail received a unilateral injection of kainate in the medial preoptic nucleus (POM). The left and right preoptic areas were collected 20 min later and assayed separately by the tritiated water technique for AA. As shown previously in preoptic explants maintained in vitro, AA was downregulated in the kainate-injected hemisphere as compared to the non-injected side. To determine whether the decline in AA detected in the POM after a sexual interaction could be mediated by an increased release of glutamate in this region, extracellular glutamate concentration was measured by in vivo microdialysis with a probe implanted in the POM of sexually mature males. Dialysate was collected every 3 minutes over three periods of 15 min when the male was (1) alone, (2) allowed to freely copulate with a female and (3) alone again. A transient rise in extracellular glutamate concentration was observed specifically and immediately after the expression of cloacal contact movements, when semen is transferred to the female. Glutamate returned to a basal level after the female was removed. Together, these results indicate that the mechanism of acute regulation of aromatase activity by glutamate identified in vitro is potentially responsible for the acute regulation of the enzyme observed in vivo following copulation. As rapid changes in brain estrogen synthesis and its actions are apparently related to the control of sexual motivation rather than sexual performance, follow up experiments should now determine whether the release of glutamate in the POM occurs in parallel with an increase in motivation or follows the termination of the copulatory sequence. [less ▲]

Detailed reference viewed: 44 (2 ULg)
Full Text
See detailSpecific properties of bone marrow mesenchymal and neural crest-derived stem cells: Relevance in spinal cord injury therapy.
Neirinckx, Virginie ULg; Agirman, Gulistan ULg; Marquet, Alice ULg et al

Poster (2014, November 17)

Spinal cord injury (SCI) treatment represents a critical issue in clinical research and patient care. Stem cell-based replacement therapies have already been proposed worldwide, especially studying stem ... [more ▼]

Spinal cord injury (SCI) treatment represents a critical issue in clinical research and patient care. Stem cell-based replacement therapies have already been proposed worldwide, especially studying stem cells from the adult bone marrow stroma. Previous studies focusing on those cells did not specifically consider their intrinsic embryonic heterogeneity, thus intermingling different stem cells subpopulations to treat experimental SCI or even injured patients. In this study, we decided to compare adult bone marrow neural crest-derived stem cells (NCSC) and mesenchymal stem cells (MSC), and highlight which of their specific properties could be relevant in therapeutic perspectives. In that purpose, we compared NCSC and MSC isolated from adult mouse bone marrow. We then compared the effects that both cell types could exert once grafted inside an injured spinal cord. Cells were injected into the spinal cord of mice that right after a spinal cord contusion at the T11-12 spinal level. Our results indicate that both MSC and NCSC-injected mice recovered locomotion abilities faster than control mice (as assessed by BMS scoring). Additionally, we observed that after 28 days post-injury, the lesion volume tended to decrease in mice that received cell graft compared to control group. Interestingly, it appeared that MSC seemed to be able to modulate inflammation inside the lesion, more than NCSC. Indeed, MSC-graft increased early neutrophil and macrophage recruitment in the bloodstream and inside the spinal cord, and increased the number of arginase-1-expressing cells remaining in the spinal cord after 28 days. In parallel, we compared the secretome of both NCSC and MSC, and noticed some interesting differences: MSC secreted several chemokines reflecting possible immunomodulating properties, while NCSC secreted products might be able to enhance neurite outgrowth. Indeed, preliminary data showed that NCSC induced neuritogenesis on primary neurons in vitro. Altogether, those results should help to improve and optimize cell-based therapies parameters and/or to define precise and efficient pharmacological treatments for SCI patients. [less ▲]

Detailed reference viewed: 71 (25 ULg)
Full Text
Peer Reviewed
See detailContrasting the role of Ih and ICaT currents in post-inhibitory rebound mechanisms in reciprocal-inhibitory networks
Dethier, Julie ULg; Drion, Guillaume ULg; Sepulchre, Rodolphe

Poster (2014, November 16)

Models with reciprocal inhibition are ubiquitous in the literature. For instance, common rhythmic motor behaviors produced by central pattern generators (CPGs) involve half-center oscillators, which ... [more ▼]

Models with reciprocal inhibition are ubiquitous in the literature. For instance, common rhythmic motor behaviors produced by central pattern generators (CPGs) involve half-center oscillators, which consist of two inhibitory neurons that are not endogenous oscillators, but produce rhythmic outputs when reciprocally connected (Marder & Calabrese 1996). Models of thalamocortical spindle oscillations also suggest that the rhythm originates from the thalamic reticular nucleus, which consists in interacting inhibitory nonoscillatory neurons (Wang & Rinzel 1992). [less ▲]

Detailed reference viewed: 40 (12 ULg)
Full Text
Peer Reviewed
See detailEffect of “diagnosis threat” in clinical setting
Fresson, Megan ULg; Dardenne, Benoît ULg; Meulemans, Thierry ULg

Poster (2014, November 14)

Objective. When reminded of their neurological history, mild traumatic brain injured (TBI) students underperform on neuropsychological tests (Suhr & Gunstad, 2002). To date, this “diagnosis threat” (DT ... [more ▼]

Objective. When reminded of their neurological history, mild traumatic brain injured (TBI) students underperform on neuropsychological tests (Suhr & Gunstad, 2002). To date, this “diagnosis threat” (DT) phenomenon has mainly been studied with a non-clinical and high-functioning population (university students). The aim of this study was twofold: to study this phenomenon with neurological patients and to examine the mechanisms responsible for underperformance. Method. Patients (18-55 years-old) who had sustained a TBI or a stroke were recruited from ambulatory and hospitalized cares, and then assigned to one of three conditions : Patients attention was drawn on (1) their neurological disease and the neuropsychological components of the upcoming tasks (DT group) ; (2) their intact sensory capacities and the sensorial components of the tasks (Neutral group); or (3) their better cognitive abilities compared to Alzheimer disease patients (Stereotype boost group). After these instructions, patients carried out cognitive tasks and completed questionnaires. Results. Preliminary analyses (n=18) showed that, on the z-score of executive functioning, the DT group performed worse than both the neutral group (p=.03) and the stereotype boost group (p=.05), but did not differ for the attentional and memory scores. Instructions also had an impact on cognitive self-efficacy, with the neutral group demonstrating greater score than the negative one (p=.08). Furthermore, the self-efficacy score tended to correlate with the score of executive functioning (r=.37). Conclusions. Results show that the DT phenomenon has an impact on cognitive performances in clinical setting, at least on executive functions, which are usually demonstrated to be the most sensitive to stereotype effects. [less ▲]

Detailed reference viewed: 31 (2 ULg)
Full Text
See detailEconomic efficiency of milk production farms in Hanoi Suburbs, Vietnam
Le Dinh, Khan; Phan Dang, Thang ULg; Lebailly, Philippe ULg et al

Poster (2014, November 14)

Dairy production has presented in Vietnam since 50 years, but these dairy farms really developed from 2001. In the period 2001 – 2010, the average growth rate of dairy herds was up to 30% per year ... [more ▼]

Dairy production has presented in Vietnam since 50 years, but these dairy farms really developed from 2001. In the period 2001 – 2010, the average growth rate of dairy herds was up to 30% per year. However, fresh milk production satisfies about 20-25% of milk consumption. The rural areas of Hanoi Province are large for dairy production. This province builds the 7 agro-ecological sub-regions for the development of dairy farming, in which Ba Vi District is a most of sub-regions for dairy production development. But the average size is about 2.5 cows per farm. This research aims to better understand how the factors in milk sector adapt to current economic conditions in Hanoi suburbs through analyzing farming systems. These studies were conducted from January to August 2012 on 43 dairy farms with different production scales at Ba Vi District to characteristic of dairy farming systems. The data were collected by interviewing and keeping records at the farms that have milk production. The results showed that three dairy production systems in these zones: (1) Dairy small farming system has from 1 to 3 cows per farm; (2) The medium farming system has from 4 to 5 cows per farm; and (3) The dairy intensive farming system has more 5 cows per farm. The economic activities were really diversified with landless per household farm such as rice production, gardens, grass production and sylviculture. Land resources per farm of intensive farming system were largest including 9,239 m² for garden around the household; 2,574 m² for rice land; 563 m² of grass area and 3,325 m² for sylviculture. While the small farming system has the lowest potential land, each household has only 2,815 m² of garden; 1,795 m² of rice land; 2,095 m² of grazing land. The medium farming system has about 3,150 m² of garden; 2,220 m² of rice land and 3,891 m² of grazing land. Milk production cycle was quite long with intensive farming system, up to 324 days comparing with 299 days per cycle with medium farming system and to 306 days per cycle with small-scale farmers. This difference was not statistically signification (P> 0.05). Average milk production yield per cow was high with medium farming system (16 kg per cow per day) in comparison with 15 kg per day at intensive and small farming systems (P> 0.05). Milk production yield has been improved in the past years with crossbreed cows such as Holstein-Friesian 50% and 75%. But this productivity was still considered to be lower than the milk production yield with dairy cows raised in Moc Chau District under Son La Province with 20.5 kg per cow per day and 6,250 kg of milk per cow per cycle). In addition, in the period from 2006 to 2012, fresh milk prices at farm gate have continued to rise for the farmers, while feed prices increased in the period from 2006 to 2010 and these feed prices were stable between 2011 and 2012. In the period of 2006 to 2012, fresh milk prices at farm gate increased from 0.17 USD per kg in 2006 to 0.60 USD per kg in 2012 and feed prices were from 0.15 USD per kg in 2006 increasing to 0.39 USD per kg in 2012. The net incomes were calculated per a cow per year reached 1,274 USD with the small-scale farming households to 1,476 USD with medium farming system. The net income per kg of fresh milk at farm gate obtained from 0.28 USD with small farming system to 0.31 USD with medium farming system. The net household income was high from dairy production, from 2,437 USD per farm per year with small farming system to 5,487 USD per farm per year with intensive farming system. The net labor family income gained from 997 USD per active per year with small farming system to 2,757 USD per active per year with intensive farming system. Thus, dairy production farms in recent years in Hanoi Suburbs provide very high economic efficiency for farmers in comparison with other agricultural activities at the farms. This explains why the rate of dairy family farming has grown very fast in the studied zones in the period from 2009 to 2012. This trend will continue to grow in the future. This research was also showed that the price of fresh milk plays a crucial role for profitable at farm level. But medium farming system with the size from 4 to 5 dairy cows per farm is more profitable with current economic conditions at farms. [less ▲]

Detailed reference viewed: 72 (1 ULg)
Full Text
Peer Reviewed
See detailWhen food cultures meet. Ethnographic analysis of belongings and positionings.
Mescoli, Elsa ULg

Poster (2014, November 13)

The displacement of human beings is always accompanied by the movement of things and practices which travel with them. This is an obvious statement that indeed leads to interesting studies on the material ... [more ▼]

The displacement of human beings is always accompanied by the movement of things and practices which travel with them. This is an obvious statement that indeed leads to interesting studies on the material culture involved by people’s mobility. Within this culture are food habits, objects of numerous scholars’ researches often focusing on culinary changes (or resistances) linked to migration paths. Far for being the only issue at stake when studying migrant’s food practices – moreover frequently assumed as originally static prior to migration –, this matter is likely to overshadow other significant dynamics. Such as those which occur when supposedly different food cultures meet, thus leading to the mobilization of belongings by groups who want to take a specific position toward one another. My paper is aimed at analysing this encounter. The assumption is that it shapes a space which corresponds to a tertium quid, that is a dimension where not only the boundaries of food cultures fade, proving of an original indeterminacy and interchangeability of practices, but also where individuals constantly change their reciprocal position depending on the perspective of the gaze upon them. To show these dynamics, I will present the ethnographic data stemmed from my doctoral research about the culinary practices of a group of Moroccan migrant women living in Milan hinterland. I will support my presentation with visual materials collected during an eighteen month fieldwork which included observations and interviews inside homes as well as in public events such as an Arab cooking class. [less ▲]

Detailed reference viewed: 23 (3 ULg)
Peer Reviewed
See detailIs there a global procedural learning deficit in children with Specific Language Impairment ?
Desmottes, Lise ULg; Maillart, Christelle ULg; Lejoly, Kelly ULg et al

Poster (2014, November 13)

The study of procedural learning abilities in children with Specific Language Impairment (Procedural Deficit Hypothesis, PDH; Ullman & Pierpont, 2005) remains a relatively unexplored field of research ... [more ▼]

The study of procedural learning abilities in children with Specific Language Impairment (Procedural Deficit Hypothesis, PDH; Ullman & Pierpont, 2005) remains a relatively unexplored field of research. Since most evidence comes from studies using tasks which involve learning of sequenced patterns, research using other procedural learning paradigms (like motor adaptation tasks) is needed to further evaluate the PDH in children with Specific Language Impairment (SLI). Therefore, in this study, we examined the ability of children with and without SLI to learn, consolidate and generalize a mirror-tracing task, a paradigm that does not involve sequence learning and had never been used in SLI. Children with SLI and typical developing (TD) matched children participated in the study. Children with SLI were included if they scored below -1.25 SD of the expected normative performance in at least 2 language areas. Both groups had to trace ten 5-pointed stars seen only in mirror-reversed view in two learning sessions separated by a one-week delay. The transfer phase consisted in tracing a new figure. The time required to complete the tracing, and the number of errors committed were recorded. Full results will be presented and discussed during the presentation of the paper. [less ▲]

Detailed reference viewed: 61 (3 ULg)
Full Text
See detailInfluence de la vitesse de croissance sur le classement de lames de bardage de Douglas wallon (Belgique)
Pollet, Caroline; Henin, Jean-Marc; Hebert, Jacques ULg et al

Poster (2014, November 12)

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a high throughput de novo sequencing platform for peptidic toxins combining proteomics and transcriptomics
Degueldre, Michel ULg; Verdenaud, Marion; Zuniga, Sheila et al

Poster (2014, November 07)

Detailed reference viewed: 20 (2 ULg)
See detailUse of lipid probes as matrices for MALDI Imaging applications
Longuespée, Rémi ULg; Alberts, Deborah ULg; Pottier, Charles et al

Poster (2014, November 07)

Detailed reference viewed: 19 (5 ULg)
Full Text
See detailComparison of Life cycle Impact Assessment methods in a case of crop in Northern France
Merchan, Angel ULg; Combelles, Agathe

Poster (2014, November 05)

Detailed reference viewed: 11 (2 ULg)
Full Text
See detailDisentangling the sources of phenotypic variation in Ambrosia artemisiifolia L.: the role of seed traits
Ortmans, William ULg; Monty, Arnaud ULg; Mahy, Grégory ULg

Poster (2014, November 03)

When invading new environments, a plant invader may express new phenotypes as a result of different ecological and genetic processes. It includes phenotypic plasticity, local adaptation, environmental ... [more ▼]

When invading new environments, a plant invader may express new phenotypes as a result of different ecological and genetic processes. It includes phenotypic plasticity, local adaptation, environmental maternal effects, and genetic drift. The quantification of each of these factors is crucial in the study of biological invasions. Ambrosia artemisiifolia L. invasion success is strongly linked to seed characteristics (dispersal by human activities, long-lived soil seed bank, etc.). Known as an opportunist and a colonizer, the species is often limited by the competition from other plants. In the early stages of development, the seedlings can be quickly outcompeted and a rapid growth is therefore a major advantage. First, this study aims to analyze the seed traits variation, and to detect an impact of these traits on the early development of the seedling (environmental maternal effect). Second, we aimed to quantify the respective role of phenotypic plasticity, environmental maternal effect, local adaptation and genetic drift on seedlings phenotype. Variability of seeds from 3 geographical zones (Belgium – Centre of France – South of France) was assessed. We measured the seed variation in mass, length, width, circularity, and pigmentation. Seeds were disposed in growth chamber under two temperature treatments. After two months, we compared seedling phenotypic variation in germination time, height, aboveground biomass, belowground biomass, early competitive performance, and the final leaf area. We found a high variability of seed traits. Seeds were varying significantly among zones, populations, and parents, with more than 30% of the variation attributable to the mother plant identity. The main sources of seedling phenotypic variation appeared to be phenotypic plasticity and environmental maternal effect. No genetic differentiation was detected in this study. Seed mass was positively correlated to seedling biomass, early competitive performance, and the final leaf area. The relevance of traits reflecting environmental maternal effect is discussed. Phenotypic plasticity and seed characteristics appear to play a major role in the invasion success. [less ▲]

Detailed reference viewed: 29 (6 ULg)
Full Text
Peer Reviewed
See detailTh17 cells in melanoma microenvironment
Weatherspoon, Alodie ULg; Multon, Sylvie ULg; Defaweux, Valérie ULg et al

Poster (2014, November)

Detailed reference viewed: 29 (9 ULg)
Full Text
See detailHighly sophisticated compound droplets on fiber arrays
Weyer, Floriane ULg; Lismont, Marjorie ULg; Dreesen, Laurent ULg et al

Poster (2014, November)

The poster shows a drop of oil containing four soapy water droplets of four different colors : red, orange, green and blue. This sophisticated object is created thanks to a fiber array. Fiber networks ... [more ▼]

The poster shows a drop of oil containing four soapy water droplets of four different colors : red, orange, green and blue. This sophisticated object is created thanks to a fiber array. Fiber networks allow processes such as droplet motion, fragmentation, trapping, releasing, mixing and encapsulation. Therefore, by using a vertical fiber decorated with successive nodes, compound droplets can be created. A soapy water droplet, with a specific color, is placed at each intersection. Then, a silicon oil droplet glides along the vertical fiber, encapsulates and collects the water droplets at each node leading to the formation of a compound droplet with four different components. [less ▲]

Detailed reference viewed: 40 (8 ULg)
See detailTreg/Th17 balance during murine embryo implantation and pregnancy
Polese, Barbara ULg; Gridelet, Virginie ULg; Araklioti, Eleni et al

Poster (2014, November)

Detailed reference viewed: 34 (9 ULg)
Full Text
Peer Reviewed
See detailIon mobility-mass spectrometry to perform structural classifications of disulfide-bridged-peptides
Massonnet, Philippe ULg; Upert, Gregory; Morsa, Denis ULg et al

Poster (2014, November)

Detailed reference viewed: 52 (25 ULg)
Full Text
See detailImpact de la phéromone d’alarme sur l’efficacité de la transmission de virus
Bosquée, Emilie ULg; Lin, Fang Jing; Liu, Ying Jie et al

Poster (2014, November)

Detailed reference viewed: 5 (1 ULg)
Peer Reviewed
See detailTMS can selectively activate and condition two different sets of excitatory synaptic inputs to corticospinal neurons in humans
Sommer, Martin; D'Ostilio, Kevin ULg; Cioccia, Matteo et al

Poster (2014, November)

Background: Current protocols or repetitive transcranial magnetic stimulation (rTMS) induce mixed facilitatory and inhibitory effects. More selective, quasi-monophasic high-frequency stimulators now ... [more ▼]

Background: Current protocols or repetitive transcranial magnetic stimulation (rTMS) induce mixed facilitatory and inhibitory effects. More selective, quasi-monophasic high-frequency stimulators now become available. We sought to investigate the impact of current direction and pulse width on intermittent theta burst stimulation (iTBS) effects on human motor cortex excitability. Also, we estimated strength-duration time constants from motor threshold and input-output (IO) curves for PA and AP orientations. Methods: We stimulated the dominant hand representation of the motor cortex in 15 healthy subjects, using “unidirectional biphasic” pulses generated by a controllable TMS machine (cTMS-3, Rogue Resolutions Ltd., Cardiff, UK), connected to a standard figure-8 coil. iTBS was applied conventionally, using 20 sequences of 2 seconds iTBS (10 bursts at 5 Hz burst repetition frequency, each burst consisting of 3 pulses of 80 % AMT intensity repeated at 50 Hz frequency). In separate sessions pulses differing in current direction and shape were applied: a) posterio-anterior (PA) current direction in the brain, 75 μs (iTBS_PA75). b) AP current direction, 45 μs (iTBS_AP45). Before and for 30 minutes after iTBS, we monitored the modulation of motor evoked potential (MEP) amplitude from the dominant first dorsal interosseus using conventional, monophasic, suprathreshold pulses generated by a Magstim 2002 stimulator, inducing PA currents in the brain, at 0.2 Hz frequency. In an additional study on ten healthy subjects, we investigated the effect the two coil orientations with three different pulse widths (30, 60 and 120 μs) on the IO curve and the latency of the motor evoked potentials (MEPs). Results: iTBS_AP45 yielded a pronounced and slightly delayed inhibition of MEP amplitude in all but one subjects, it was unrelated to the MEP latency differences. iTBS_PA75 had a variable and inconsistent effect that was in part related to the latency differenceAP-LM , in that long latency differences were correlated with the induction of inhibition rather than facilitation. We found a longer time constant for AP than PA orientation. MEP latencies yielded an interaction between pulse width and orientation, due mainly to longer onset latencies following AP stimuli of short duration. Conclusions: Current direction influences the outcome of iTBS, with a preference for AP currents. PA and AP stimuli activate the axons of neurones with different time constants. Those activated by AP pulses excite corticospinal outputs with a longer latency than those activated by PA pulses. AP pulses of short duration recruit long latency inputs most selectively. [less ▲]

Detailed reference viewed: 84 (4 ULg)
Full Text
See detailDistribution and robustness of a distance-based multivariate coefficient of variation
Aerts, Stéphanie ULg

Poster (2014, November)

When one wants to compare the homogeneity of a characteristic in several popula- tions that have di erent means, the advocated statistic is the univariate coe cient of variation. However, in the ... [more ▼]

When one wants to compare the homogeneity of a characteristic in several popula- tions that have di erent means, the advocated statistic is the univariate coe cient of variation. However, in the multivariate setting, comparing marginal coe cients may be inconclusive. Therefore, several extensions that summarize multivariate relative dispersion in one single in- dex have been proposed in the literature (see Albert & Zhang, 2010, for a review). In this poster, focus is on a particular extension, due to Voinov & Nikulin (1996), based on the Mahalanobis distance between the mean and the origin of the design space. Some arguments are outlined for justifying this choice. Then, properties of its sample version under elliptical symmetry are discussed. Under normality, this estimator is shown to be biased at nite samples. In order to overcome this drawback, two bias corrections are proposed. Moreover, the empirical estimator also su ers from a lack of robustness, which is illustrated by means of in uence functions. A robust counterpart based on the Minimum Covariance Determinant estimator is advocated. [less ▲]

Detailed reference viewed: 29 (8 ULg)
Full Text
Peer Reviewed
See detailImpact of the dialysis membrane on the Vitamin D metabolims markers
CAVALIER, Etienne ULg; DUBOIS, Bernard ULg; Urena, Pablo et al

Poster (2014, November)

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailSystematic Analysis of two cystatin C assays using samples of 2057 older adults - The Berlin initiative study
Ebert, natalie; DELANAYE, Pierre ULg; Martus, Peter et al

Poster (2014, November)

Detailed reference viewed: 15 (2 ULg)
Peer Reviewed
See detailAmmonia oxidizing bacteria community structure and richness under coniferous/deciduous tree species at three temperate forest sites
Malchair, Sandrine ULg

Poster (2014, November)

Ammonia Oxidizing Bacteria community structure and richness under coniferous/deciduous tree species at three temperate forest sites Malchair S. and Carnol M. Laboratory of Plant and Microbial Ecology ... [more ▼]

Ammonia Oxidizing Bacteria community structure and richness under coniferous/deciduous tree species at three temperate forest sites Malchair S. and Carnol M. Laboratory of Plant and Microbial Ecology Department Biology, Ecology and Evolution University of Liege, Belgium Introduction: Despite the crucial roles of soil microorganisms in ecosystem processes, numerous uncertainties subsist on the relationship between soil microbial diversity and function. Furthermore, the link between aboveground and belowground diversity remains also unclear. In European forests, at the beginning of the 19th century, plantations of Norway spruce and Scots pine for timber production were substantially increased. However, concerns were expressed with regard to the ecological risks posed by coniferous monocultures. The conversion of these monocultures into broadleaved or mixed stands has been suggested as a solution. Nevertheless, belowground effects of such a change in the dominant tree species is largely unknown, although bacteria regulate many soil processes and some groups, like ammonia oxidizing bacteria (AOB) are highly sensitive to environmental stresses. Objectives: The aims of this study were to investigate (i) AOB community structure and richness under several tree species, (ii) microbial/environmental factors influencing AOB diversity, (iii) the relationship between AOB diversity and the nitrification process. Materials and methods: Forest floor (Of, Oh) was sampled under Norway spruce, Douglas fir, European beech and sessile oak at three temperate forest sites. AOB diversity (community structure and richness) was assessed by PCR-DGGE and sequencing. Samples were analysed for microbial (net N mineralization, potential nitrification, basal respiration, microbial biomass, microbial or metabolic quotient) and environmental parameters (pH, total nitrogen, extractable ammonium, organic matter content and exchangeable cations). Results: AOB sequences retrieved in this study were related to different uncultured strains from soils, related to both Nitrosospira-like and Nitrosomonas-like sequences. AOB community structure and tree species effects on AOB diversity were site-specific. AOB community structure was influenced by environmental/microbial parameters, i.e. net N mineralization or organic matter content, regulating ammonium availability. AOB richness was not related to nitrification but a very weak correlation between potential nitrification and AOB community structure was observed. Conclusions: At larger spatial scales, site specific characteristics may be more important that tree species in determining AOB richness and community structure. However, within sites, tree species influence AOB diversity. The absence of a clear relationship between AOB diversity and nitrification points to a possibly role of AOB abundance, phenotypic plasticity or the implication of ammonia oxidizing archaea in this process. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailAnalysing spatiotemporal changes in sediment contamination
Richir, Jonathan ULg; Pini, Jennifer; White, Shannon et al

Poster (2014, November)

Coastal environments are subjected to anthropogenic threats, of which pollution by trace elements (TEs). They remain chemicals of concern because of their toxicity, their ability to be concentrated in ... [more ▼]

Coastal environments are subjected to anthropogenic threats, of which pollution by trace elements (TEs). They remain chemicals of concern because of their toxicity, their ability to be concentrated in biota and their persistence in sediment. Aware of these threats, monitoring agencies perform large environmental surveys. However, the databases generated often remain underexploited, even though they represent an important source of information for scientists. As a case study, we focused on the highly industrialized and developed coastal area of the Solent, south coast of the UK, which is also a European Marine site with protected habitats and species. To date, no scientific-based spatiotemporal trend has been published regarding its pollution in TEs. But mining of existing databases requested from the Environmental Agency and the Marine Environment Monitoring and Assessment National database showed that sediment from 32 sites were/are indeed monitored for that purpose, covering a 22 years period (1992-2013). Temporal trend analysis showed that sediment contamination significantly decreased during that time interval (45 % decrease in median). However, all TEs still show concentrations above Sediment Quality Guidelines. Important spatial variability is also present, presumably linked to the distribution of pollutant anthropogenic sources. Taken together, these data have been used to provide stakeholders with relevant scientific based tools, i.e. GIS maps of the contamination in TEs in the Solent. This regional-level assessment enables local stakeholders to create bridges between environmental scientists and local authorities and valorizes the existing databases, for future governance at the regional and national scales. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
See detailRobust detection of local outliers in multivariate spatial data
Ernst, Marie ULg

Poster (2014, November)

Multivariate spatial data are geographical locations on which non spatial variables are measured. Such data may contain two types of outliers: global and/or local. Focus is here on local outlier whose ... [more ▼]

Multivariate spatial data are geographical locations on which non spatial variables are measured. Such data may contain two types of outliers: global and/or local. Focus is here on local outlier whose attribute values lie far from the values taken by its neighbors. This poster has three main objectives. The first is to review some local detection techniques that seem to perform well in practice. Secondly, an adaptation to one of these is suggested to further develop its local characteristic. Then simulations based on Matérn model are reported and discussed in order to compare in an objective way the different detection techniques. [less ▲]

Detailed reference viewed: 10 (5 ULg)
Full Text
Peer Reviewed
See detailEtude de la forme d'arcade dentaire après nivellement en technique Damon.
LEWY, Stéphanie; LIMME, Michel ULg; BRUWIER, Annick ULg

Poster (2014, November)

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailMagnetospheric modes and magnetic reconnection.
Hubert, Benoît ULg; Milan, S.E.; Cowley, S.W.H.

Poster (2014, November)

We combine imaging of the proton aurora from the SI12-IMAGE instrument with measurement of the ionospheric convection from the SuperDARN radar network to analyze the cycle of magnetic flux opening and ... [more ▼]

We combine imaging of the proton aurora from the SI12-IMAGE instrument with measurement of the ionospheric convection from the SuperDARN radar network to analyze the cycle of magnetic flux opening and closure of the Earth magnetosphere. Interaction between the solar wind and the Earth geomagnetic environment causes a reconfiguration of the magnetic field that connects the interplanetary magnetic field (IMF) to the geomagnetic field. This reconnection process produces open magnetic field lines (i.e. field lines of the magnetosphere that close through the interplanetary medium) that are dragged to the magnetotail by the solar wind flow, where they eventually reconnect again, back to a closed topology. The SI12 imaging of the Doppler-shifted Lyman-α emission of the proton aurora is used to estimate the location of the boundary separating open and closed field lines at ionospheric altitude. We then estimate the open magnetic flux of the Earth magnetosphere, encircled by this boundary. The rate of reconnection causing a variation of the open magnetic flux can be expressed as a voltage in application of Faraday’s law. This voltage is measured along the open/closed field line boundary determined from the imaging data. The electric field associated with the voltage has two origins: motion of the boundary and the ionospheric field. We use the ionospheric electric field deduced from ionospheric convection measurement from the SuperDARN to estimate the reconnection voltage at the magnetopause (flux opening) and in the magnetotail (flux closure) accounting for the motion of the open/closed field line boundary determined from the SI12 images. The method is applied during substorms, steady geomagnetic convection intervals, sawtooth events and geomagnetic storms. These different intervals are characterized by different values of open flux and reconnection rates, as a result of different coupling between the solar wind and the geomagnetic environment. We interpret these differences as different dynamic modes of the magnetospheric system. Shock-induced flux closure events are also presented, as an exceptional situation that differs from the modes presented above. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailDifferent activities of chemokines CXCL12 and vCCL2 and peptides derived from their N-terminus towards the receptors CXCR7/ACKR3 and CXCR4
Szpakowska, Martyna ULg; Gauthier, Pierre-Arnaud; Derj, Anouar et al

Poster (2014, November)

Detailed reference viewed: 15 (1 ULg)
Full Text
See detailMicrowave-assisted extraction of pectin from unused pumpkin biomass
Košťálová, Zuzana; Aguedo, Mario ULg; Hromádková, Zdenka

Poster (2014, November)

Detailed reference viewed: 20 (0 ULg)
Full Text
See detailComputergestützte Förderung der mündlichen Leistung im Fremdsprachenunterricht - Die @lter Fremdsprachkurse an der Universität Lüttich (ULg), Belgien
Thonard, Audrey ULg; Jérôme, Françoise ULg; Badir, Yasmine ULg et al

Poster (2014, October 30)

The poster présents the implementation of voice tools in the service of foreign language learning at the University of Liège. Oral skills (comprehension and production) are trained online in autonomy and ... [more ▼]

The poster présents the implementation of voice tools in the service of foreign language learning at the University of Liège. Oral skills (comprehension and production) are trained online in autonomy and with instructors' interventions. The setting also provides facilities for oral feedbacks. [less ▲]

Detailed reference viewed: 19 (4 ULg)
See detailRecyclage des briques cassées pour améliorer les propriétés céramiques des argiles plastiques (Meknès, Maroc)
El Ouahabi, Meriam ULg; Daoudi, Lahcen; El Boudour El Idrissi, Hicham ULg et al

Poster (2014, October 27)

Dans le Bassin de Saïs (nord ouest du Maroc), les argiles du Miocène de la région de Meknès sont utilisées dans l’industrie céramique locale pour la fabrication des briques. Ces argiles riches en smectite ... [more ▼]

Dans le Bassin de Saïs (nord ouest du Maroc), les argiles du Miocène de la région de Meknès sont utilisées dans l’industrie céramique locale pour la fabrication des briques. Ces argiles riches en smectite et en carbonates, ont une granulométrie très fine, marquée par la quasi absence de la fraction sableuse. Ces propriétés les rendent très plastiques, ce qui engendre des taux de retrait importants au séchage et à la cuisson et des problèmes de qualité du produit fini qui s’en suivent (fissurations, déformations et casses pendant les processus de séchage et de cuisson). Dans le but de palier à ce problème et d’améliorer la qualité du produit fini, nous proposons au cours de cette étude de recycler les déchets de briques cassées. Afin d'atteindre ces objectifs, 4 formulations ont été réalisées à base d’argiles plastiques, riches en smectite et des casses de briques broyées. Des quantités de 5, 10, 15 et 20% de briques broyées ont été ajoutées à la pâte destinée à la confection des briques. Des analyses minéralogiques par diffraction des rayons X (DRX), chimiques par spectrométrie de fluorescence (XRF), granulométrique par granulométrie laser, ainsi que les limites d’Atterberg ont été effectuées. Les résultats minéralogiques montrent une diminution de la teneur en carbonates (calcite) et en argile totale (principalement smectite), lors des ajouts successifs de briques broyées. La fraction sableuse augmente progressivement, jusqu’à atteindre 30% pour une formulation avec 20% de casses de briques. Cette granulométrie plus grossière diminue la plasticité de la pâte et permet d’éviter les déformations et les fissures pendant le processus du séchage. De plus, l’ajout des casses de briques dilue les carbonates, ce qui permet d’améliorer la qualité du produit cuit. Ces résultats indiquent que le recyclage des déchets de briques, présente un intérêt à la fois économique et écologique. Des essais de comportement au séchage et à la cuisson ainsi que des essais mécaniques complèteront cette étude. [less ▲]

Detailed reference viewed: 83 (20 ULg)
Full Text
See detailReformage catalytique du toluène
Claude, Vincent ULg; Lambert, Stéphanie ULg; Courson, Claire

Poster (2014, October 23)

This poster summarizes results obtained for the catalytic reforming of tar in the oultlet gas of a biomass gasifier. All samples were synthesized with aluminum nitrate and an organosilane (EDAS) in order ... [more ▼]

This poster summarizes results obtained for the catalytic reforming of tar in the oultlet gas of a biomass gasifier. All samples were synthesized with aluminum nitrate and an organosilane (EDAS) in order to enhance the metallic dispersion and surface area. Three different compositions have been studied (Al2O3-SiO2; Al2O3-SiO2+2%wt Ni; Al2O3-SiO2+10%wt Fe). The catalytic tests highlight the fact that the sample containing 2%Ni wt is the more appropriate in our conditions. [less ▲]

Detailed reference viewed: 19 (5 ULg)
Peer Reviewed
See detailAnalysis of the interactions of natural elicitor rhamnolipids with plant plasma membranes by in silico methods
Polo Lozano, Damien ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2014, October 20)

Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various ... [more ▼]

Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various chain lengths linked through a glycosidic bond to one or two rhamnose moieties. The fatty acids are linked together through an ester bond. These molecules have shown several biological activities including plant defense stimulation. It has be suggested that this elicitor activity could be related to an interaction of rhamnolipids with the lipid bilayer of the plant plasma membrane (PPM) and lead to its destabilization, which can activate the plant defense signaling pathways. In this context, interactions of two rhamnolipids (Rha-C10-C10 and Rha-Rha-C10-C10) with membrane models and lipidic constituents of the PPM were investigated using in silico approaches. Most probable chemical structures of the rhamnolipids were determined using the STRUCTURE TREE procedure according to the molecule potential energy. The ability of these rhamnolipid structures to insert within the PPM was assessed using IMPALA simulations. IMPALA uses a membrane model in which phospholipids molecules are implicitly modeled by an empirical function and the membrane properties are modeled by energetic restraints. The ability of each rhamnolipid structure to form an assembly with several PPM constituents (phospholipid (PLPC), sterols (Sitosterol, Stigmasterol, Campesterol) and sphingolipids (GIPC, Glucosylceramide)) was calculated using the HYPERMATRIX procedure, which calculate and minimize the energies of interaction between all molecules of the complex until the lowest energy structure is reached. [less ▲]

Detailed reference viewed: 56 (5 ULg)
Peer Reviewed
See detailSurfactin interaction with model plant plasma membrane
Luzuriaga Loaiza, Walter ULg

Poster (2014, October 20)

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailCharacterization of collagen fibrils after equine suspensory ligament injury: an ultrastructural and biochemical approach
Shikh Al Sook, Mohamad Khir ULg; Gabriel, Annick ULg; Salouci, Moustafa et al

Poster (2014, October 19)

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril ... [more ▼]

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to the fibril morphology, as well as the collagen content and types. The purpose of this study was to evaluate, using biochemical and ultrastructural approaches, the alterations in collagen fibrils after injury. Eight Warmblood horses with visible signs of injury in only one forelimb SL were selected and specimens were examined by transmission electron microscope (TEM). Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin. TEM revealed abnormal organization as well as alterations in the diameter and shape of fibrils after SL injury. The bands corresponding to types I, III and V collagen were assessed by densitometry after sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric analysis indicated that the proportions of type III and type V collagen were significantly higher (P <0.001) in damaged tissues compared to normal tissues with a mean increase of 20.9 and 17.3% respectively. Concurrently, a significant decrease (P <0.001) in type I collagen within damaged tissues was recorded with a mean decrease of 15.2%. These alterations could be the hallmark of a decrease in the tissue quality and mechanical properties of the ligament. This provides new insight for subsequent research on tissue regeneration that may lead to the development of future treatment strategies for SL injury. [less ▲]

Detailed reference viewed: 226 (63 ULg)
Full Text
See detailRole of non-polyQ regions on the aggregation process by polyQ proteins into amyloid fibrils
Huynen, Céline ULg; Willet, Nicolas ULg; Buell, Alexander K et al

Poster (2014, October 18)

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated ... [more ▼]

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated proteins. This polyQ expansion is thought to be the major determinant in the development of neurotoxicity, triggering protein aggregation into amyloid fibrils. A large body of evidence however indicates that non-polyQ regions modulate the aggregation process triggered by polyQ expansions. The interplay between the polyQ tract and non-polyQ regions is complex and still not fully understood. In order to better understand it, we previously designed and characterized model polyQ proteins made of the beta-lactamase BlaP and a 23, 30, 55 or 79Q tract inserted in position 197 or 216. These chimeras recapitulate the aggregation properties of polyQ disease-associated proteins: there is a Q-threshold for the formation of amyloid fibrils, and above the threshold, the longer the polyQ, the faster the aggregation. Moreover, the structure of BlaP (native or unfolded) and the position of insertion of the polyQ tract (197 versus 216) influence their aggregation properties. In this work, (i) we discuss the role of the conformation of the host protein, BlaP, and of the location of the polyQ within BlaP on the different phases of amyloid fibril formation, the nucleation and elongation steps, using mainly quartz crystal microbalance (QCM), atomic force microscopy (AFM). Our results highlight a linear dependence of the polyQ length on the elongation rate whatever the insertion site and the conformation of BlaP. These two parameters however drastically influence the ability of a polyQ tract to trigger the nucleation and the elongation steps of amyloid fibril formation. (ii) Finally, we investigate the precise aggregation threshold and the modulating role of the N- and C-terminal polyQ flanking sequences in position 197 of BlaP by creating and characterizing new chimeras containing intermediate length polyQ tracts in position 197, or polyQ tracts inserted between two cleavage sites in position 197, respectively. We observe that the propensity to trigger the full process of amyloid fibril formation and its rate seems to be largely dependent on the polyQ length and on the polyQ flanking sequences. Altogether our results contribute to identify the important species and elements (polyQ or non-polyQ regions, monomers, oligomers or fibrils) during the aggregation process into amyloid fibrils to interfere with the latter associated with neurotoxicity. [less ▲]

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailDetermination of COBALT in patients with metal hip prosthesis
MISTRETTA, Virginie ULg; Charlier, Corinne ULg

Poster (2014, October 18)

Detailed reference viewed: 17 (2 ULg)
Full Text
Peer Reviewed
See detailCase report : Rhabdomyolysis following acute alcohol intoxication
HALENG, Jeanine ULg; CAVALIER, Etienne ULg

Poster (2014, October 18)

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailEffet de l'expertise musicale sur la perception de la justesse vocale
Gosselin, Laura; Morsomme, Dominique ULg; Larrouy, Pauline ULg

Poster (2014, October 17)

Detailed reference viewed: 21 (1 ULg)
Full Text
See detailEvaluation of morphological and functional characteristics of Carnobacterium maltaromaticum isolated from vacuum-packaged beef with long shelf life
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Ndedi Ekolo, François et al

Poster (2014, October 17)

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or ... [more ▼]

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or genera of bacteria. The presence of certain lactic acid bacteria adapted to a low temperature in fresh meat could extend the shelf life and improve the microbial stability and safety of this product. The aim of this study was to perform a morphological and functional characterization of a C. maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged beef with very long shelf life. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). Among the studied conditions, a temperature of +12 °C and an atmosphere poor in oxygen were optimal for the growth of C. maltaromaticum. Vacuum packing is therefore suitable for this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this isolate will be further pursued by a genotypic characterization. Special attention will be taken to study its bioprotective properties. [less ▲]

Detailed reference viewed: 16 (5 ULg)
Full Text
See detailImpact of aging technique and muscle on oxidative stability of beef packaged under high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Thimister, Jacqueline ULg et al

Poster (2014, October 17)

Two common approaches for beef aging are wet-aging and carcass-aging. Wet-aging refers to meat aged in a sealed vacuum package at refrigerated temperatures, while carcass aged at controlled temperatures ... [more ▼]

Two common approaches for beef aging are wet-aging and carcass-aging. Wet-aging refers to meat aged in a sealed vacuum package at refrigerated temperatures, while carcass aged at controlled temperatures and humidity is defined as carcass-aging. Carcass-aging is an ancient process used nowadays to produce beef characterized by its superior quality. The meat conservability is influenced by its sensitivity to oxidative process which can vary from one muscle to another. The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on colour and lipid stability of beef packaged in high-oxygen atmosphere. After a seven-day wet- or carcass-aging step, longissimus dorsi and rectus femoris muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. At different times, part of these samples was repackaged under modified atmosphere – 70 % O2:30 % CO2 –, and stored during 7 days at +4 °C in order to simulate retail conditions. The following parameters were evaluated: colour (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS), antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase), alpha-tocopherol and fat content. The sensitivity of high-oxygen atmosphere repacked meat cuts to oxidation was influenced by the aging technique (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. Oxidation stability could be associated with muscle catalase activity, and no association could be established with the alpha-tocopherol content. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailMélancolie et dépression : étude phénoménologique
Ramackers, Amélia; Englebert, Jérôme ULg

Poster (2014, October 17)

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailAlveolar macrophages hyporeactivity act in the extreme susceptibility of DBA/2J to Influenza A infection.
Casanova Bustos, Tomas Ronaldo ULg; Garigliany, Mutien-Marie ULg; Desmecht, Daniel ULg

Poster (2014, October 17)

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining ... [more ▼]

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining this difference, mainly by genetic approaches using Recombinant Inbred Lines between those two strains. Several candidate-genes have been proposed, but it was not possible to determine their importance. To help to define the factors involved in the susceptibility of DBA/2J mice to influenza infection, we adopted a phenotypic approach to identify the critical steps of the infection process accounting for this extreme susceptibility. Overall, the data presented here support the role of a dysfunction of alveolar macrophages, to influenza infection in the higher susceptibility of DBA/2J mice to this virus. [less ▲]

Detailed reference viewed: 15 (4 ULg)
See detailIn vitro study of coinfection/superinfection parameters which can influence recombination events in noroviruses
Di Felice, Elisabetta; Ceci, Chiara; Toffoli, Barbara et al

Poster (2014, October 17)

Noroviruses (NoVs) are non-enveloped, single-stranded, positive-sense RNA viruses. They are important causes of acute non-bacterial gastroenteritis in humans worldwide but their study is currently yet ... [more ▼]

Noroviruses (NoVs) are non-enveloped, single-stranded, positive-sense RNA viruses. They are important causes of acute non-bacterial gastroenteritis in humans worldwide but their study is currently yet hampered by the lack of a cell culture system. NoVs genetically evolve by both point mutations and recombination and the murine norovirus (MuNoV) is considered as the best model for human NoVs. The aim of this study was to develop an experimental model based on the MuNoV in order to investigate coinfection/superinfection parameters that could impact recombination events. Monolayers of RAW264.7 cells were coinfected or superinfected with two MuNoV strains (CW1 and WU20) using different multiplicity of infection (0.1/1; 1/1 and 10/1 for CW1 and Wu20 , respectively) and time delays (0h; 0.5h; 1h; 2h; 4h; 8h; 12h and 24h) for infection. Supernatants were collected at 24 and 48 hours post-infection. Genomic copies of both viruses were first quantified by RT-QPCR. Then, viruses from the supernatants were plaque purified (36 clones per condition) and their recombinant status was checked by a real-time PCR discriminating method using primers targeting both extremity of the MuNoV genome. Results of quantitative and plaque picking assays are compared. Together, the results confirm that recombination does not frequently occur, at least in vitro and raise the issue on why these events are however so usual with in silico detection methods. The data also showed that superinfection exclusion seems to be triggered from 4h post infection with the first MuNoV. The mechanisms of the later should be still studied. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailEffect of bovine colostrum on growth and survival in Red kids during the first year of life.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

Poster (2014, October 17)

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted ... [more ▼]

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted in Secondary Goat Breeding Center of Maradi (SGBCM). Forty newborn kids, regardless of sexe, were divided randomly into two groups: a "T-Control " group receiving only breast milk per feeding (n = 20) and a "C-Colostrum" group having the same diet but supplemented with bovine colostrum thawed (50 ml the first day of life, then 25 ml in 2 meals/ day between the ages of 2 and 15d, n = 20). The results indicate that supplementation with bovine colostrum in the first 15 days of life, increases the growth of kids until weaning (P<0.001), modifie some barymetric changes some settings improves health status and reduces the mortality rate. To our knowledge, in the studied environment, this work is original and seems to bring new knowledges likely to have practical applications in areas with farming conditions quite difficult. [less ▲]

Detailed reference viewed: 38 (8 ULg)
See detailVascular endothelial growth factor: a blood biomarker in canine idiopathic pulmonary fibrosis.
Roels, Elodie ULg; Krafft, Emilie ULg; Laurila, HP et al

Poster (2014, October 17)

Detailed reference viewed: 22 (2 ULg)
See detailFitness evolution of a recombinant murine norovirus during serial passages in cell culture
Oliveira-Filho, Edmilson; Di Felice, Elisabetta; Toffoli, Barbara et al

Poster (2014, October 17)

Human norovirus (NoV) infections are among the most important causes of gastroenteritis in both children and adults and often occur as outbreaks which may be foodborne. Recombination can dramatically ... [more ▼]

Human norovirus (NoV) infections are among the most important causes of gastroenteritis in both children and adults and often occur as outbreaks which may be foodborne. Recombination can dramatically change virulence properties of the viruses and has been often evidenced in silico for different NoV strains. Recently, after in vitro coinfection of RAW264.7 cells with parental murine norovirus (MuNoV) strains CW1 and Wu20, we obtained a recombinant Wu20/CW1 strain (Mathijs et al., 2010). This recombinant strain showed reduced plaque size compared to the parental strains. The aim of the study was to observe and molecularly characterize the natural genetic evolution of the recombinant MuNoV strain across in vitro replications. The recombinant strain was serially replicated in vitro (up to 14 passages). Viral plaque diameters of early and late progenies were compared with the Image software. A significant difference was shown between them with the Mann and Whitney non parametric statistical test. The average size of plaques increased from the earlier to the later progenies (from 0.1 mm2 to around 0.5 mm2). Molecular investigations are currently performed in order to specify in which genetic region mutations occur and whether or not this could explain fitness modifications during in vitro evolution. In addition two other parameters of in vitro virulence modification will be investigated (i) virus production and (ii) one step growth kinetics. The data should provide interesting information about genetic evolution in the genus Norovirus, especially regarding recombination events and explain how a recombinant strain, first disadvantaged compared to its parental strains, could regain fitness by genetic evolution. Mathijs, E., Muylkens, B., Mauroy, A., Ziant, D., Delwiche, T., Thiry, E., 2010. Experimental evidence of recombination in murine noroviruses. J Gen Virol 91, 2723-2733. [less ▲]

Detailed reference viewed: 6 (0 ULg)
See detailPhenotyping Belgian Blue cattle for their susceptibility to psoroptic mange
Abos, Romain ULg; Coussé, Annelies; Sarre, Charlotte et al

Poster (2014, October 17)

Detailed reference viewed: 20 (1 ULg)
Full Text
See detailThe Effect of Urtica dioica Supplements on Egg Quality
Touazi, Leghel; Moula, Nassim ULg

Poster (2014, October 17)

Eggs are a rich source of lipids and proteins. Because of their low production cost, they represent a cheap source of animal proteins and lipids. recently, in Algeria, consumers’ interest are oriented of ... [more ▼]

Eggs are a rich source of lipids and proteins. Because of their low production cost, they represent a cheap source of animal proteins and lipids. recently, in Algeria, consumers’ interest are oriented of healthiness and dietetic values food. The purpose of this study was to investigate the effects a dietary supplement of Urtica dioica on laying hen's performance and egg quality. The study was conducted from may to june 2014 in the area of Chemini (Algeria). Sixty, 25-wk-old Lohmann Brown layers were randomly divided into 3 groups with 20 hens in each group. Group 1 was the control group fed a standard commercial diet based on corn and soybean, whereas the groups 2 and 3 were fed the control diet supplemented with 1% and 1.5% of nettle respectively. The hens were housed in cages in the same local and fed ad libitum. Fifty five eggs were sampled 4 times in each group from 25 to 27 weeks. A total of 220 eggs were examined. A series of measurements were carried out on each egg, namely egg weight, form index (egg shape), yolk to albumen ratio (Y:A), Yolk color, egg shell thickness, Haugh’s units. Egg weight, yolk color and Haugh Units (HU) were affected by age. Significant differences (P<0.05) were recorded between feeding type in total egg weight, yolk color and Haugh Units. The egg from group 2 proved to have a higher egg weight (57.67g; group 1: 55.41g; group 3: 56.70g; P<0.05), (33.4%), intermediate Yolk color (8.53; group 1: 9.03 ; group 3: 9.38; P<0.05) and HU (79.81; group 1: 80.85; group 3: 76.92). The results showed that the use of 1% and 1.5% of Urtica dioica, had positive effects on the color of yolk of laying hens. Using different levels of Urtica dioica could change the characteristics of egg and could meet the preferences of consumers for the intense color of the yolk. [less ▲]

Detailed reference viewed: 39 (11 ULg)
Full Text
See detailLocal goat in Kabylie (Chemini and Bouzeguene)
Moula, Nassim ULg; Michaux, Charles ULg; Philippe, François-Xavier ULg et al

Poster (2014, October 17)

The livestock sector plays a dynamic role in the economic development of rural areas in Algeria. Due to its adaptation to harsh environments, goat breeding is practiced in many areas of the country. To ... [more ▼]

The livestock sector plays a dynamic role in the economic development of rural areas in Algeria. Due to its adaptation to harsh environments, goat breeding is practiced in many areas of the country. To the north, it is confined to mountainous areas, but the bulk of the workforce is left in the steppe and semi-desert areas. The goat population in Algeria reached 3.8 million heads, ranking second after sheep. The present work contributes to a better characterisation of local goat in Kabylie for production and reproduction traits. The study was conducted in the regions of Chemini (Bejaia province) and Bouzeguene (Tizi-Ouzou province). A survey was conducted in 29 herds. The results revealed that the overall mean of goats per households was 6.69 ± 2.97. The purposes of keeping goat in Chemini and Bouzguene was private consumption of milk (82.76%) and meat (68.97%) as well as for commercial exchange (62.07%). The Kabyle goat is small (male: 62.23 cm and female: 58.41 cm) with long hair. Its ears are drooping, its convex profile has a slightly pronounced nasal split and its coat colour ranges from dark brown to black. However, the crossbreeding with exotic breeds (mainly with Saanen goat), controlled or uncontrolled, increased the frequency of white coat. The average age at puberty in males was 6.93 ± 1.04 and 7.38 ± 0.94 months in females. The reported age at first kidding and kidding interval were 13.03 ± 0.87 months and 7.83 ± 1.29 months, respectively. [less ▲]

Detailed reference viewed: 38 (13 ULg)
See detailEffect of biocides on murine norovirus and feline calicivirus, surrogates of human norovirus, in suspension, glove and stainless steel disc tests
Zonta, William ULg; Mauroy, Axel ULg; Thiry, Etienne ULg

Poster (2014, October 17)

Human noroviruses (HuNoV) are one of the major agents of human gastroenteritis and transmission occurs mainly by the faecal-oral route. The purpose of this work was to test biocide products on surrogate ... [more ▼]

Human noroviruses (HuNoV) are one of the major agents of human gastroenteritis and transmission occurs mainly by the faecal-oral route. The purpose of this work was to test biocide products on surrogate viruses of HuNoV in order to get information on the residual viral infectivity and on the integrity of viral genomes after biocide treatment in various conditions. Murine norovirus (MNN) and feline calicivirus (FCV) have been chosen as HuNoV surrogates because presenting comparable structure and physico-chemical properties. Two biocide products have been chosen, Ethanol (70%) and Kenocid 2100® (Peracetic acid and hydrogen peroxide) and used in 3 different conditions (in suspension, on gloves and on stainless steel discs). The biocide product was tested according to Afnor norm EN 14476. The reduction of viral titre was inferred and RNA extraction followed by a 1 step RT-qPCR was performed. If biocides products tested are able to show a 4 log reduction of the viral infectious titre, they are considered as effective. Efficacy against HuNoV can be extrapolated from these results. MNV is sensitive to Ethanol and Kenocid 2100® with and without any effect on genomic copy numbers respectively. FCV is sensitive to Kenocid 2100® with an effect on genomic copy numbers but is resistant to EtOH. The absence of effect of Kenocid 2100® on genomic copy numbers of MNV indicates this biocide product does not interfere directly on the viral genome. It may likely act on the viral structure, the capsid for example. This project is financed by Federal Public Service, Health, Food Chain Safety and Environment, in Belgium (RT 10/6 TRAVIFOOD). [less ▲]

Detailed reference viewed: 12 (0 ULg)
Full Text
See detailThe effect of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler
Moula, Nassim ULg; Philippe, François-Xavier ULg; Michaux, Charles ULg et al

Poster (2014, October 17)

The objective of this study was to evaluate the effects of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler chicken. The study was ... [more ▼]

The objective of this study was to evaluate the effects of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler chicken. The study was conducted from May to June 2014 in the area of Chemini (Algeria). In a completely randomized design, a total of 100 one-day-old broilers (industrial strain) were divided into 2 groups and 2 repetitions with 25 chickens each. Group 1 was the control group fed a standard commercial diet based on corn and soybean purchased on the local market, whereas the birds in group 2 were fed the nettle diet (control diet with addition of 2% of nettle). The animals were housed inside in experimental pens and fed ad libitum. The results showed that nettle inclusion significantly promotes growth performance of broilers at 42 days of age (1644.8 ± 45.5 vs. 1565.1 ± 45.5g; P<0.05). The broilers fed the nettle diet had higher thigh yield (26.0 ± 0.3 vs. 25.2 ± 0.3%; P<0.05) but lower abdominal fat yield (4.39 ± 0.16 vs. 3.90 ± 0.16%; P<0.05). No significant effect of the diet was observed for carcass yield (around 71.1%) and breast percentage (around 32.40%). The overall mortality was not significantly different (P>0.05) for any of the dietary regimens (around 20%). Feed conversion ratio of the two groups were very close (around 2.17). This experiment showed that dietary inclusion of Urtica dioica has positive effects on growth performance and carcass quality of broilers. [less ▲]

Detailed reference viewed: 32 (9 ULg)
Full Text
Peer Reviewed
See detailL'intolérance environnementale idiopathique attribuée aux champs électromagnétiques (IEI-EMF)
Demaret, Isabelle ULg

Poster (2014, October 16)

Depuis le début des années '80, des chercheurs étudient les plaintes des personnes se disant hypersensibles aux champs électromagnétiques (EMF), sous les normes recommandées. Les recherches n'ont ... [more ▼]

Depuis le début des années '80, des chercheurs étudient les plaintes des personnes se disant hypersensibles aux champs électromagnétiques (EMF), sous les normes recommandées. Les recherches n'ont cependant pas démontré que ces personnes étaient capables de détecter les EMF ni qu'elles présentaient des changements physiologiques en étant exposées aux EMF . L'OMS a regroupé ces plaintes sous le nom d'Intolérance Environnementale Idiopathique attribuée aux Champs Electromagnétiques (IEI-EMF). Au niveau médical, l'IEI-EMF est classée parmi les syndromes fonctionnels somatiques comme d'autres syndromes sans bases physiologiques démontrées. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
See detailQuantitative Texture Analysis of melt-cast processed Bi-2212 superconductors
Dellicour, Aline ULg; Vertruyen, Bénédicte ULg; Chateigner, Daniel et al

Poster (2014, October 15)

Bi2Sr2CaCu2O8+δ HTSC superconductor is characterized by a very strong normal-state resistivity anisotropy, with ρc/ρab typically above 104. The aim of this study is to use Quantitative Texture Analysis ... [more ▼]

Bi2Sr2CaCu2O8+δ HTSC superconductor is characterized by a very strong normal-state resistivity anisotropy, with ρc/ρab typically above 104. The aim of this study is to use Quantitative Texture Analysis from neutron diffraction measurements to estimate the orientation effect on the macroscopic resistivity (ρM) in melt-cast bulk Bi2Sr2CaCu2O8+δ superconductors. Our approach uses the geometric mean (GMA) [1] of the single crystal resistivity tensor (ρSC) weighted by the Orientation Distribution Function (ODF) to quantitatively estimate the macroscopic resistivity tensor of the samples. The ODF is obtained from neutron Combined Analysis [2], using the E-WIMV algorithm of the MAUD software. The GMA applies to the rank-two resistivity tensor of the orthorhombic space group considered tetragonal due to the small difference of a- and b-axes of the phase, with only two independent tensor components. We relate a relatively good agreement between measured and calculated macroscopic resistivity ratios. [less ▲]

Detailed reference viewed: 14 (2 ULg)
See detailIncrease in northern hemisphere stratospheric hydrogen chloride over recent years
Mahieu, Emmanuel ULg; Chipperfield, MP; Notholt, J et al

Poster (2014, October 07)

Detailed reference viewed: 15 (2 ULg)
Full Text
Peer Reviewed
See detailSolar irradiance modelling over Belgium using Regional Climate Models within the frame of a day-ahead photovoltaic production forecasting system
Beaumet, Julien ULg; Doutreloup, Sébastien ULg; Fettweis, Xavier ULg et al

Poster (2014, October 06)

WRF-ARW and MAR climate models performances for the modelling of solar irradiances over Belgium are evaluated using in-situ measurements at Sart-Tilman and Daussoulx. Different WRF-ARW settings are tested ... [more ▼]

WRF-ARW and MAR climate models performances for the modelling of solar irradiances over Belgium are evaluated using in-situ measurements at Sart-Tilman and Daussoulx. Different WRF-ARW settings are tested. Sigmoid model proposed by Ruis-Ariaz etal. (2010) is used to decompose solar irradiance into direct and diffuse fraction. The performance of this model using measured and modelled global irradiances is also evaluated. [less ▲]

Detailed reference viewed: 78 (10 ULg)
Full Text
Peer Reviewed
See detailPotential Proteomic Biomarkers Associated To Mucosal Healing In Crohn’s Disease
MEUWIS, Marie-Alice ULg; Baiwir, Dominique ULg; Mazzucchelli, Gabriel ULg et al

Poster (2014, October 06)

Introduction and objectives: In Crohn's disease (CD), there is a discrepancy between clinical activity of the disease (symptoms) and intestinal healing. However absence of tissue healing is associated ... [more ▼]

Introduction and objectives: In Crohn's disease (CD), there is a discrepancy between clinical activity of the disease (symptoms) and intestinal healing. However absence of tissue healing is associated with the risk of relapse and tissue damage progression. Endoscopy is costly and invasive. Hence biomarkers correlating with intestinal healing could improve disease management. We aimed to identify potential biomarkers associated to CD mucosal healing by a shotgun proteomics label free study. Methods: We used the STORI clinical trial cohort (n=103) aiming at identifying markers associated to relapse prediction after Infliximab treatment withdrawals. We used serum samples of patients in clinical remission, grouped according to the degree of intestinal healing seen at endoscopy. We performed depletion of the 20 most abundant plasma proteins on each serum pools and ran a proteomics label free differential analysis using 2D-nanoUPLC-MSE HDMS Synapt G1 for data acquisition and Protein Lynks Global Server vs 2.4 for data analysis (Waters, Corp., Milford, USA). Results and Discussion: We obtained potential biomarkers and designed a multiplexed -selected reaction monitoring (SRM) method for validation of these candidates in each individual patient. The method may also be tested in an independent set of IBD patients with and without mucosal healing. Conclusions: This research strategy and results of SRM validation of potential biomarkers associated to mucosal healing in this cohort of CD patients as well as the tests done on other CD patients, may provide new opportunities for CD follow-up tests development. [less ▲]

Detailed reference viewed: 53 (11 ULg)
Full Text
See detailNEAR-DEATH EXPERIENCES IN PATIENTS WITH LOCKED-IN SYNDROME
Charland-Verville, Vanessa ULg; Lugo Ramirez, Zulay del Rosario ULg; Jourdan, Jean-Pierre et al

Poster (2014, October 04)

Near-Death Experiences (NDEs) are classically associated with positive emotions like peacefulness, happiness and joy [1-3]. To date, few negative NDEs reports have been documented [4]. Although NDEs ... [more ▼]

Near-Death Experiences (NDEs) are classically associated with positive emotions like peacefulness, happiness and joy [1-3]. To date, few negative NDEs reports have been documented [4]. Although NDEs classically arise in the context of an acute severe brain damage, their associated memories are reported as being phenomenologically very rich and detailed [5]. To date, no satisfactory explanatory model exits to fully account for the rich phenomenology of NDEs following a severe acute brain injury [6]. Neurobiological hypotheses include cerebral hypoxia [7, 8] and temporal lobe dysfunctions [9] to account for some of the features occurring during NDEs. However, it has been recently shown that anoxic/hypoxic, traumatic and other supratentorial brain lesions do not seem to influence the content of a NDE when assessed with a standardized tool (i.e., Greyson NDE scale; [1]). Due to their particular brain lesion (i.e., pontine brainstem), locked-in syndrome (LIS) patients provide a unique opportunity to further investigate the neural correlates of NDEs. We here aimed at retrospectively characterizing the content of NDEs in patients with LIS having suffered from an acute brainstem lesion (cerebrovascular accident (CVA) or trauma; i.e., “LIS NDEs”) and to compare these experiences to those collected in a cohort of matched NDE experiencers after coma with supratentorial lesions (CVA or trauma; i.e., “classical NDEs”). [less ▲]

Detailed reference viewed: 94 (0 ULg)
Full Text
Peer Reviewed
See detailA propos de deux cas de manipulation de patients dans le cadre de recherches cliniques
Jamoulle, Marc ULg

Poster (2014, October)

L’exercice de la prévention quaternaire, soit veiller à ne pas nuire au patient, est mis ici à contribution dans deux cas de recherches cliniques. Deux patients ont vécu dans le même service de ... [more ▼]

L’exercice de la prévention quaternaire, soit veiller à ne pas nuire au patient, est mis ici à contribution dans deux cas de recherches cliniques. Deux patients ont vécu dans le même service de cardiologie une tentative de manipulation lors de recherches, l’une pharmacologique, l’autre de traitement invasif. Le questionnement des patients a été discutés au cours de la consultation de médecine générale. Les deux patients ont chacun relu et compris la portée de leur témoignage publié ci-dessous et marquent leur accord formel à leur publication. [less ▲]

Detailed reference viewed: 83 (0 ULg)
Full Text
See detailDesigning large-scale CO2 capture units with assessment of solvent degradation
Léonard, Grégoire ULg; Crosset, Cyril; Dumont, Marie-Noëlle ULg et al

Poster (2014, October)

Solvent degradation is a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. In the present work, we refine a ... [more ▼]

Solvent degradation is a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. In the present work, we refine a previous kinetic model for describing solvent oxidative and thermal degradation based on experimental results. The CO2 capture process is then modeled in Aspen Plus with assessment of solvent degradation. As a result, this work provides a useful tool for the identification of optimal operating conditions that minimize both the energy and environmental impacts of the process. [less ▲]

Detailed reference viewed: 33 (2 ULg)