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See detailEOF analysis of long-term reconstructed AVHRR Pathfinder SST in the South China Sea
Huynh, Thi Hong Ngu ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2014, May 02)

Sea surface temperature (SST) is one of the key variables often used to investigate ocean dynamics, ocean-atmosphere interaction, and climate change. For recent decades, the AVHRR Pathfinder SST, measured ... [more ▼]

Sea surface temperature (SST) is one of the key variables often used to investigate ocean dynamics, ocean-atmosphere interaction, and climate change. For recent decades, the AVHRR Pathfinder SST, measured by infrared sensors, has been widely used because of its high resolution and long time-series. The disadvantage of the AVHRR Pathfinder SST is high percentage of missing data due to cloud coverage. This becomes more serious in the South China Sea (SCS) because it is located in the tropical region, frequently covered by clouds. In this study, we used the Data INterpolating Empirical Orthogonal Functions (DINEOF) method to reconstruct daily night-time 4 km AVHRR Pathfinder SST spanning from 1989 to 2009 for the whole SCS. In order to better understand the spatial and temporal variability of the SCS SST, an EOF analysis of the reconstructed field is performed in association with surface wind. The first SST mode, accounting for 69% of the variance, presents the cooling (warming) of the basin due to the solar inclination through seasons, water exchange, topography, and monsoon-induced cyclonic circulation. The second SST mode, explaining 24.8% of the variance, shows the advection of cold and warm water from two opposite directions along the southwest-northeast diagonal of the basin. The second SST mode is affected by the atmospheric anticyclone (cyclone) located over the Philippine Sea. Comparing both SST modes with Nino3.0 index, it shows that the interannual variability of the SCS SST is influenced by the moderate and strong ENSO events with a lag of 5-6 months. Moreover, the analysis of the high-resolution reconstructed dataset reveals some oceanic features that could not be captured in previous EOF analyses. [less ▲]

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See detailEffect of metal nanoparticles encapsulated in porous silica on the biphenyl biodegradation by Rhodococcus erythropolis T902.1
Wannoussa, Wissal ULg; Hiligsmann, Serge ULg; Heinrichs, Benoît ULg et al

Poster (2014, May 01)

Biphenyl is stable and highly hydrophobic, thus having a low availability for degrading microorganisms, which need an aqueous environment for their growth. As a consequence, bioremediation processes are ... [more ▼]

Biphenyl is stable and highly hydrophobic, thus having a low availability for degrading microorganisms, which need an aqueous environment for their growth. As a consequence, bioremediation processes are very limited. However, a few studies showed that using a low metal concentration accelerates the biodegradation of pollutants (Yeom and Yoo1997). Nanoparticles are considered as a new generation of compounds to improve environmental remediation and biological processes (Beckers et al. 2013; Zhang 2003). This paper investigated the enhancement effect of nanometre-sized metallic Cu, Ag, Pd or Co, on the biphenyl biodegradation by Rhodococcus erythropolis T902.1. Have been synthesized by a sol–gel process (Lambert et al. 2004). These nanoparticles (NP) of about 2–3 nm were encapsulated in porous silica (SiO2) and were added at low concentration (10-4M) in the M284 minimal medium with 500 ppm biphenyl as source of carbon and energy. The cultures containing Pd or Co produced 30% more biphenyl degraded with a higher Rhodococcus growth than those without NP (positive control) or with silica particles only. On the contrary, the presence of 10-4 M Cu or Ag nanoparticles showed an inhibitory effect on bacterial growth and biphenyl degradation compared to the positive. [less ▲]

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See detailBias correction using data assimilation: Application on the Lorenz ’95 and NEMO-LIM models.
Canter, Martin ULg; Barth, Alexander ULg

Poster (2014, May 01)

Data assimilation has been used for decades in fields like engineering or signal processing to improve forecast models. Ensemble Kalman filters and other sequential data assimilation methods are examples ... [more ▼]

Data assimilation has been used for decades in fields like engineering or signal processing to improve forecast models. Ensemble Kalman filters and other sequential data assimilation methods are examples of developments which reduce the uncertainty of the model by taking observations into account. The widespread interest in addressing systematic forecast model errors only arose when the advances in modelling, data assimilation and computational power had reduced random errors to the point of commensurability with systematic errors, also known as bias. We present here a new method of bias correction using data assimilation. This method is based on the stochastic forcing of a model to correct bias. First, through a preliminary run, we estimate the bias of the model and its possible sources. Then, we establish a forcing term which is directly added inside the model’s equations. We create an ensemble of runs and consider the forcing term as a control variable during the assimilation of observations. We then use this analysed forcing term to correct the bias of the model. Since the forcing is added inside the model, it acts as a source term, unlike external forcings such as wind. This procedure has been developed and successfully tested with a twin experiment on a Lorenz 95 model. Indeed, we were able to estimate and recover an artificial bias that had been added into the model. This bias had a spatial structure and was constant through time. The mean and behaviour of the corrected model corresponded to those the reference model. It is currently being applied and tested on the sea ice ocean NEMO LIM model, which is used in the PredAntar project. NEMO LIM is a global and low resolution (2 degrees) coupled model (hydrodynamic model and sea ice model) with long time steps allowing simulations over several decades. Due to its low resolution, the model is subject to bias in area where strong currents are present. We aim at correcting this bias by using perturbed current fields from higher resolution models and randomly generated perturbations. [less ▲]

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See detailEffects of two PBDE congeners on the moulting enzymes of the freshwater amphipod Gammarus pulex.
Gismondi, Eric ULg; Thomé, Jean-Pierre ULg

Poster (2014, May)

Polybrominated diphenyl ethers (PBDEs) constitute a class of chemical compounds included to the composition of usual products such as plastics, textiles or electrical equipment, due to their flame ... [more ▼]

Polybrominated diphenyl ethers (PBDEs) constitute a class of chemical compounds included to the composition of usual products such as plastics, textiles or electrical equipment, due to their flame retardants properties. Since 2004, PBDEs were banned in Europe and listed as Priority Substances within the European Union Water Frame Work Directive. Unfortunately, the release of PBDEs always occurs in ecosystems due to their presence in products currently in use and new products manufactured using recycled PBDE-containing material. However, only few studies have investigated their impacts on freshwater invertebrates. This work aimed to study the effects of BDE-47 and BDE-99 congeners on the chitobiase and chitinolytic enzymes activities of the freshwater amphipod Gammarus pulex, according to gender, PBDE concentration and time of exposure. In addition, the bioaccumulation of BDE-47 and BDE-99 were measured. The results revealed that there was a dose-response relationship for the PBDE accumulation in G. pulex, whatever the gender and the PBDE congeners. Female G. pulex bioaccumulated more PBDE than males, and especially BDE-99 which was more accumulated than BDE-47. Moreover, PBDE exposures for 96hrs have caused the inhibition of chitobiase and chitinolytic enzymes activities. This study not only indicate the importance of taking into account various confounding factors (gender, congeners, concentration) to understand the PBDE effects, but underline also disruptions of enzymes activities involved in the molting process. These disturbances suggest effects on the gammarid development and reproduction, and consequently on the gammarid population. [less ▲]

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See detailBenfotiamine increases cell proliferation in adult mouse hippocampus
Vignisse, Julie ULg; Caron, Nicolas; Malgrange, Brigitte ULg et al

Poster (2014, May)

Because of the rising prevalence of Alzheimer’s disease (AD), new strategies to slow, halt and prevent the neurodegenerative process are urgently needed. Recently, it has been shown that chronic ... [more ▼]

Because of the rising prevalence of Alzheimer’s disease (AD), new strategies to slow, halt and prevent the neurodegenerative process are urgently needed. Recently, it has been shown that chronic administration of benfotiamine, a thiamine (vitamin B1) - precursor, in a mouse model of AD (APP/PS1 mice) significantly reduced key features of this disease, namely, memory impairment, β-amyloid accumulation and tau hyperphosphorylation [1]. Pyrithiamine, an antimetabolite of thiamine, administered to the same transgenic model has the opposite effects on Alzheimer’s features [2]. However, mechanisms underlying these effects are still unknown. As decreased neurogenesis and consequent cognitive decline have been linked to Alzheimer’s disease [3], we hypothesized that beneficial effects of benfotiamine could be mediated by increased hippocampal neurogenesis. Therefore, we investigated whether benfotiamine (200 mg/kg/day) or thiamine (200 mg/kg/day) orally administered to 3-month old C57BL wild-type mice for 20 days could affect hippocampal neurogenesis. For that purpose, mice were sacrificed 2 h, 24 h and 3 weeks after BrdU injection and immunohistochemistry was performed on brains in order to study cell proliferation, cell cycle exit, neuron survival and integration. Our results show that benfotiamine, but not thiamine treatment, increased both the number of proliferative progenitors (Ki67+ cells) and the number of immature neurons (BrdU+ cells). The difference between thiamine and benfotiamine can be explained by the fact that the latter has a much better bioavailability than thiamine. Indeed, after administration of benfotiamine, blood thiamine concentrations can increase by a factor 100 or more [4]. Further studies will be needed to explain how increased circulating thiamine concentrations can affect neurogenesis. References: 1. Pan X., Gong N., Zhao J., Yu Z., Gu F., Chen J., Sun X., Zhao L., Yu M., Xu Z., Dong W., Qin Y., Fei G., Zhong C., Xu T. L., (2010) Brain 133, 1342-1351. 2. Zhao J., Sun X., Yu Z., Pan X., Gu F., Chen J., Dong W., Zhao L., Zhong C. (2010) Neurotox Res 19, 575-583. 3. Lazarov O. and Marr R.A., (2013) Front. Aging Neurosci. 5, 43. 4. Volvert M.L., Seyen S., Piette M., Evrard B., Gangolf M., Plumier J.C., Bettendorff L. (2008) BMC Pharmacol 8, 10. [less ▲]

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See detailEvent justice and social entity justice: A cross-lagged analysis
Marzucco, Laurence ULg; Hansez, Isabelle ULg

Poster (2014, May)

ABSTRACT This study aimed to examine the directionality of the relationship between event justice and social entity justice. Using two samples and a cross-lagged panel analysis with two measurement times ... [more ▼]

ABSTRACT This study aimed to examine the directionality of the relationship between event justice and social entity justice. Using two samples and a cross-lagged panel analysis with two measurement times, social entity justice was found to predict event justice (procedural, distributive, interpersonal and informational justice event), supporting the top-down process. PRESS PARAGRAPH The life of organizations is punctuated by a wide range of managerial decisions (e.g., hiring, performance appraisal or organizational change). Facing such events, employees evaluate the fairness of the situation experienced. However, little is known about how employees develop justice judgments. This study shows that employees’ fairness perceptions about their organization influence their interpretation of the fairness of subsequent events involving the organization. Building and fostering a climate of justice is therefore of primary importance for organizations, since global fairness perceptions about the organization may help employees to perceive a specific event, such as a managerial decision, as being fair. [less ▲]

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See detailEVALUATION OF THE RAPID DETECTION OF ST-17 AND ST-1 GROUP B STREPTOCOCCI USING A MICROFLEX MALDI-TOF MS (BRUKER)
MEEX, Cécile ULg; SACHELI, Rosalie ULg; DESCY, Julie ULg et al

Poster (2014, May)

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to ... [more ▼]

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to evaluate an easy and rapid method, recently described to detect ST-17 and ST-1 GBS, based on distinguishing peak-shifts present on the protein spectrum of these 2 sequence types, using a Microflex (Bruker) matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS). Methods This study was performed on 67 multi locus sequence typed (MLST) GBS originated from the Belgian and Czech National Reference Centers, including 18 ST-17 and 16 ST-1. After culture on blood agar, an ethanol/formic acid extraction was performed on each strain. Each extract was spotted once on a target plate, overlaid with 1 µl alpha-cyano-4-hydroxycinnamic acid matrix and further analysed by a Microflex MALDI-TOF MS. One spectrum per isolate was recorded, 240 laser shots being recorded for each spectrum. The spectra were further analysed using a Bruker prototype software, and 2 logarithmic values, one for ST-17 and one for ST-1, calculated from the intensities of the present and absent peaks, were obtained for each strain. If >0, this value indicated the presence of the specific sequence type. In a second step, the test was repeated on each strain with discordant result when compared with MLST. Results Compared with MLST method, the first analysis of the strains gave poor results, leading to very low sensitivities (77.8% for ST-17 and 50% for ST-1) but rather good specificities (85.7% for ST-17 and 98.0% for ST-1). After repeating the analysis on the strains with discordant result, sensitivity, 100% and 93.8%, and specificity, 87.8% and 98.0%, for ST-17 and ST-1 respectively were highly improved. Conclusion Since ST-17 and ST-1 GBS both show distinguishing peak-shifts on their protein spectrum, as described by Lartigue et al., the distinction of these 2 sequence types is now possible by MALDI-TOF MS. To our knowledge, this study is the first describing this application on a Microflex MS using a software to classify the strains. The observed results are promising but, given to the variability of the logarithmic value given by the software, the need to perform several measures on a same strain seems to be essential. After optimization of the analysis procedure, this rapid, easy and cheap method could be used to precociously detect ST-17 among GBS isolated from prenatal screenings, allowing a better follow up of the colonized mothers and a closer monitoring of their newborns. We would like to thank the Bruker Company which allowed us to evaluate the prototype software they have developed. [less ▲]

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See detailGrowth Factors-Induced Angiogenesis Requires uPAR on Endothelial Cells
Paques, Cécile ULg; Herkenne, Stéphanie ULg; Pollenus, Thomas et al

Poster (2014, May)

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See detailOutplacement adequacy and benefits: The mediating role of retrospective justice
Marzucco, Laurence ULg; Hansez, Isabelle ULg

Poster (2014, May)

ABSTRACT We examined the relationships between perceived outplacement adequacy, retrospective justice and outplacement benefits for redundant employees using outplacement services. Based on an ... [more ▼]

ABSTRACT We examined the relationships between perceived outplacement adequacy, retrospective justice and outplacement benefits for redundant employees using outplacement services. Based on an outplacement experience perceived as adequate, victims formed retrospective justice perceptions about their former organization that in turn, impacted their negative emotions, well-being, future perspectives and job search. PRESS PARAGRAPH The increasing prevalence of organizational downsizing has been matched by growth in the provision of outplacement services over the last decade. Despite this rapid growth and ongoing need for outplacement services, little is yet known about the perceived adequacy and the benefits of these services for redundant employees using them. Our results indicated that an outplacement experience perceived as adequate for redundant employees retrospectively fosters their impressions of justice about the dismissing organization; this leads in turn to satisfactory benefits for them: reduction of negative emotions, enhancement of their perceived well-being, future perspectives, and job-seeking activities. [less ▲]

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See detailFemmes auteures d’homicide en détention : des liens d’attachement aux ruptures de liens
Glowacz, Fabienne ULg; Bernardello, Sandrine; Résimont, Isabelle

Poster (2014, May)

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See detailWork-home interference and well-being: A cross-lagged analysis.
Babic, Audrey ULg; Stinglhamber, Florence; Barbier, Marie ULg et al

Poster (2014, May)

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See detail25KDa-Thiamine triphosphatase is essential for spermatozoid development in mice
Kohn, Grégory ULg; Ectors, Fabien ULg; Lakaye, Bernard ULg et al

Poster (2014, May)

For many years, our laboratory has been interested in thiamine triphosphate (ThTP), a vitamin B1 derivative whose metabolism and physiological role remain unclear. Regarding its production, we have shown ... [more ▼]

For many years, our laboratory has been interested in thiamine triphosphate (ThTP), a vitamin B1 derivative whose metabolism and physiological role remain unclear. Regarding its production, we have shown that in E. coli and in rat brain mitochondria, ThTP is synthesized from thiamine diphosphate and inorganic phosphate through a chemiosmotic mechanism involving the FoF1- ATP synthase [1, 2]. In mammalian cells, its concentration is maintained at a low level through hydrolysis by a very specific cytosolic 25-kDa thiamine triphosphatase (ThTPase) [3]. In order to gain insight in the role of ThTP and ThTPase in mammalian tissues, we decided to generate a mouse strain invalidated in 25kDa-ThTPase with the hope that these mice will accumulate ThTP in their tissues. We obtained genetically modified embryonic stem (ES) cells from the Knockout Mouse Project (KOMP) repository. In those cells, one of the 25kDa-ThTPase alleles was replaced by a construction containing the lacZ and the neomycin resistance genes. Those ES cells were microinjected in blastocysts and the chimeric blastocysts were injected in a mouse uterus to generate chimerae. However, when we bred those mice with wild type mice, the construction was never transmitted to the pups. To explain this result, we selected those chimerae that presented a sex-reversal. In those mice, all the spermatozoids derive from the injected embryonic stem cells, so that half of the spermatozoids are expected to harbor the construction. However, after qPCR analysis, we observed that the spermatozoids with the construction were outnumbered by a factor of thousand. These results strongly suggest that the 25kDa-ThTPase is required for spermatozoid development. 1. Gangolf, M., Wins, P., Thiry, M., El Moualij, B. & Bettendorff, L. (2010) J. Biol. Chem. 285, 583-94. 2. Gigliobianco, T., Gangolf, M., Lakaye, B., Pirson, B., von Ballmoos, C., Wins, P. & Bettendorff, L. (2013) Scientific reports. 3, 1071. 3. Lakaye, B., Makarchikov, A. F., Antunes, A. F., Zorzi, W., Coumans, B., De Pauw, E., Wins, P., Grisar, T. & Bettendorff, L. (2002) J. Biol. Chem. 277, 13771-7. [less ▲]

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See detailThiamine and benfotiamine improve memory, decrease depressive-like behavior and reduce brain expression of GSK3 beta in mice
Markova, N; Shevtsova, E; Vignisse, Julie ULg et al

Poster (2014, May)

An increasing body of evidence suggests an implication of altered thiamine metabolism in mental illnesses, including depression and Alzheimer’s disease. Here, we examined the effects of a two-week ... [more ▼]

An increasing body of evidence suggests an implication of altered thiamine metabolism in mental illnesses, including depression and Alzheimer’s disease. Here, we examined the effects of a two-week treatment with thiamine (200 mg/kg/day) or benfotiamine (200 mg/kg/day, a synthetic thiamine precursor of high bioavailability, on learning of C57 mice in step down avoidance (SDA) and fear conditioning (FC) tests and depressive-like behavior in the modified forced swimming test (modFST). In addition, we investigated the effects of these drugs on brain gene expression of Glycogene Synthase Kinase 3beta (GSK3b), a regulatory factor that is involved in both above-mentioned mental conditions. Benfotiamine, but not thiamine significantly increased contextual learning in C57 mice in the step-down paradigm and acquisition of fear conditioning during weak training. Both compounds have improved the extinction of contextual fear conditioning memory and did not alter the acquisition of fear conditioning during strong training. Also, we found that both treatments reduced depressive-like behavior in mice, as shown by diminished duration of floating behavior in the modified forced swim test. With the modified forced swimming test applied here, an additional swimming session was carried out 120 hours after the first trial and resulted in increased mRNA levels of GSK3b in the prefrontal cortex, which correlated with duration of floating in tested mice. Thiamine and benfotiamine reduced GSK3b expression in the prefrontal cortex and the hippocampus that was more pronounced in the animals with low floating, in case of the measurements made in the prefrontal cortex. Together, these data suggest pro-cognitive and antidepressant-like effect of benfotiamine that are partly shared by thiamine and can be mediated by their inhibitory effect on the GSK3b [less ▲]

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See detailL'enfant placé au carrefour de ses appartenances
Aubinet, Suzanne ULg

Poster (2014, May)

Michard (2009) nous dit de la loyauté qu’elle est l’armature de l’appartenance et à Neuburger de souligner qu’en plus d’être un socle sur lequel l’identité se structure, l’appartenance contient et limite ... [more ▼]

Michard (2009) nous dit de la loyauté qu’elle est l’armature de l’appartenance et à Neuburger de souligner qu’en plus d’être un socle sur lequel l’identité se structure, l’appartenance contient et limite les effets des relations (2003). Pour Onnis (2008) c’est dans la dialectique entre identité et appartenance que naît l’individualité qui, elle, se réalise dans la relation (Dolto, 1988). Plymackers (2006) insiste sur les notions d’espaces où se nouent ces relations mais aussi de temps où ces dernières se jouent. L’enfant, par des mouvements dialectiques d’allées et venues avec son/ses système(s) d’appartenances tisse pas à pas les prémices de son identité. Ses relations structurent et étayent ce processus et la temporalité constitue le squelette de celui-ci. Mais qu’en est-il de ces enfants placés, maltraités, déplacés ? Qu’en est-il si le conflit ou le clivage de loyauté entre famille(s) et institution(s) rend l’étayage de l’appartenance ardu ? Qu’en est-il si la discontinuité des espaces et des relations prédomine et si le processus d’individuation est suspendu par une séparation arbitraire dictée par un autrui qui « n’appartient pas » ? L’analyse clinique de cas d’enfants aux vécus chaotiques rythmés par de nombreux placements nous permet de décortiquer les particularités de ce processus complexifié par ces ruptures d’espaces, ces loyautés morcelées, ces appartenances diffuses et d’en cerner les impacts. [less ▲]

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See detailSEDIMENTS OXIDATION BY SEAGRASSES: INFLUENCE ON THE S CYCLE IN POSIDONIA OCEANICA (L.) DELILE INTERMATTES DYNAMIC
Abadie, Arnaud ULg; Lejeune, Pierre; Pergent, Gérard et al

Poster (2014, May)

The Mediterranean seagrass Posidonia oceanica forms meadows and develops a complex of rhizomes, roots and sediment which is called “matte”. P. oceanica meadows show discontinuity patterns in the form of ... [more ▼]

The Mediterranean seagrass Posidonia oceanica forms meadows and develops a complex of rhizomes, roots and sediment which is called “matte”. P. oceanica meadows show discontinuity patterns in the form of sand or dead matte (matte without living shoots) patches, called “intermattes”, which can have natural or anthropogenic origins. Mechanical processes (e. g. currents, anchoring) can initiate intermattes formation but their dynamic after creation seems to be linked with the sediment chemistry, especially with S cycle. P. oceanica plays an important role in controlling coastal belowground biogeochemistry, in particular by oxidizing sediments through the release of O2 by roots. This process allows creating more suitable condition for plant growth and colonization. The lack of H2S oxidation in SO4 2- can lead to limitation of the plant development or its regression. In order to investigate the effect of oxidation condition in sediments on intermattes dynamic and the neighboring meadow, we initiate, in December 2013, a study on six intermattes (three natural, three anthropogenic) at different depths in Calvi Bay, in Corsica (France). We hypothesize that redox potential and H2S concentration in sediments play an important role in the regression of P. oceanica meadows, particularly after a mechanical anthropic impact like anchoring. It also may be possible that two different kinds of processes are involved for each type of intermatte. Regular samplings throughout two years are planed with the aim of evaluating the seasonal variations of physicochemical parameters. [less ▲]

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See detailSeeking for causes of recent methane increase: comparison between GEOS-Chem tagged simulations and FTIR column measurements above Jungfraujoch
Bader, Whitney ULg; Bovy, Benoît ULg; Wecht, K et al

Poster (2014, May)

Atmospheric CH4 reached 260% of the pre-industrial level (~700 ppb) due to increased emissions from anthropogenic sources. Globally averaged CH4 reached a new high of 1819 ± 1 ppb in 2012, an increase of ... [more ▼]

Atmospheric CH4 reached 260% of the pre-industrial level (~700 ppb) due to increased emissions from anthropogenic sources. Globally averaged CH4 reached a new high of 1819 ± 1 ppb in 2012, an increase of 6 ppb with respect to the previous year (WMO, Greenhouse gas Bulletin N.9, 2013). CH4 above Jungfraujoch increases at 0.53±0.19%/year during the late 90s to stabilize and reach a non significant trend from 2000 to 2005. Since 2006, atmospheric methane has been continuously increasing with a rate of 0.19±0.05 %/year. The attribution of this increase to any CH4 source is difficult since the current network is insufficient to characterize emissions by region and source process, emphasizing the need for source-tagged model simulations as it should provide us information on processes causing the increase of atmospheric methane since 2005/2006. [less ▲]

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See detailQuantitative multi-parameter mapping in parkinson’s disease: preliminary results
Rouillard, Maud ULg; D'Ostilio, Kevin ULg; Albinet, Cedric et al

Poster (2014, May)

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See detailEpidemiology of VAP and VAC
LAYIOS, Nathalie ULg; DAMAS, Pierre ULg

Poster (2014, May)

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See detailDioxins and PCBs in eel and Chinese mitten crabs in the Rhine-Meuse estuary
van Hattum, B; Nijssen, P; Focant, Jean-François ULg et al

Poster (2014, May)

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See detailLong-term evolution and seasonal modulation above Jungfraujoch (46.5°N, 8.0°E): Optimisation of the retrieval strategy, comparison with model and independant observations
Bader, Whitney ULg; Stavrakou, J; Muller, J-F et al

Poster (2014, May)

Methanol (CH3OH) is the second most abundant organic compound in the Earth’s atmosphere after methane. In this work, we present the first long-term time series of methanol total, lower tropospheric and ... [more ▼]

Methanol (CH3OH) is the second most abundant organic compound in the Earth’s atmosphere after methane. In this work, we present the first long-term time series of methanol total, lower tropospheric and upper tropospheric-lower stratospheric partial columns derived from the analysis of high resolution Fourier transform infrared solar spectra recorded at the Jungfraujoch station (46.5°N, 3580 m a.s.l.). The retrieval of methanol is very challenging due to strong absorptions of ozone in the region of the selected 8 band of CH3OH. Two wide spectral intervals have been defined and adjusted in order to maximize the information content. Methanol does not exhibit a significant trend over the 1995-2012 time period, but a strong seasonal modulation characterized by maximum values and variability in June-July, minimum columns in winter and a peak-to-peak amplitude of 130 %. In situ measurements performed at the Jungfraujoch and ACE-FTS occultations give similar results for the methanol seasonal variation. The total and lower tropospheric columns are also compared with IMAGESv2 model simulations. There is no systematic bias between the observations and IMAGESv2 but the model underestimates the peak-to-peak amplitude of the seasonal modulations. [less ▲]

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See detailCorrelation between composition and cold stability of palm liquid fractions.
Danthine, Sabine ULg; Lefebure, Emilie ULg; Dijkmans, Peggy et al

Poster (2014, May)

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See detailA new analysis of the optical polarisation alignments of quasars
Pelgrims, Vincent ULg

Poster (2014, April 30)

Introduction of a new statistical method dedicated to the analysis of polarisation orientations and presentation of some results of its application to the sample of optical polarisation measurements of ... [more ▼]

Introduction of a new statistical method dedicated to the analysis of polarisation orientations and presentation of some results of its application to the sample of optical polarisation measurements of 355 quasars. [less ▲]

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See detailTRAPPIST detection of the light from a bloated hot Jupiter at the edge of tidal disruption
Delrez, Laetitia ULg; Gillon, Michaël ULg; Lendl, Monika ULg et al

Poster (2014, April 30)

Abstract : We present here the discovery by the WASP-­South survey, in close collaboration with the Euler and TRAPPIST telescopes, of the transiting planet WASP-­121b as well as the measurement of its ... [more ▼]

Abstract : We present here the discovery by the WASP-­South survey, in close collaboration with the Euler and TRAPPIST telescopes, of the transiting planet WASP-­121b as well as the measurement of its thermal emission at 0.9 microns. WASP-­121b is a very inflated (1.76 RJup) Jupiter-­mass (1.02 MJup) planet that transits every 1.27 days a bright F6V star. It is remarkable as its orbital radius is only ~10% larger than its Roche limit, suggesting that it might experience mass loss through Roche-­lobe overflow. Thanks to its large size and extreme irradiation (~7 10^9 erg s-1 cm-­2), it was predicted to display a thermal emission of ~0.1% of the stellar flux in the near-­infrared. Using the TRAPPIST robotic telescope, we could detect this thermal emission signal at ~5 sigma in the z'-­band. This measurement, a first for a ground-­based 60cm telescope, allows to place preliminary constraints on the atmospheric properties of this very special hot Jupiter. [less ▲]

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See detailEpigenetic regulation of macrophage polarisation
Hamaïdia, Malik ULg; Cosse, Jean-Philippe ULg; Willems, Luc ULg

Poster (2014, April 29)

There are two main macrophage subsets based on their cytokine pattern and phenotype: classical (or M1) and alternative (or M2). M1 macrophages promote anti-tumor immunity by supporting polarization of CD4 ... [more ▼]

There are two main macrophage subsets based on their cytokine pattern and phenotype: classical (or M1) and alternative (or M2). M1 macrophages promote anti-tumor immunity by supporting polarization of CD4 T-lymphocytes into anti-tumor Th1 and Th17. On the other hand, tumor associated macrophages (TAMs) that are close to M2 promote survival and proliferation of tumor cells. Evidence indicates that macrophage polarization is mediated by a transcriptional program that is influenced by epigenetic modifications. We investigated the effect of different epigenetic inhibitors on polarization of human macrophages. After isolation of human peripheral blood mononuclear cells, macrophages were polarized into M1 (using LPS+IFN-gamma) or M2 (with IL4) in presence or absence of inhibitors. Flow cytometry analyzes showed that epigenetic modulation affects CD206 expression on M2 macrophages, dextran-FITC phagocytosis and proliferation of allogeneic T-lymphocytes. Epigenetic inhibitors thus affect polarisation into M2 and may be useful to improve immunotherapy of cancer. [less ▲]

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See detailVadose zone studies at an industrial contaminated site: the vadose zone monitoring system and cross-hole geophysics
Fernandez de Vera, Natalia ULg; Beaujean, Jean ULg; Jamin, Pierre ULg et al

Poster (2014, April 29)

Poster presented at the European Geoscience Union General Assembly 2014. In this poster, the installation of the vadose zone experimental set up is presented along with first results

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See detailLD-based haplotype encoding scheme with iterative pruning principal component analysis (ipPCA) to retrieve population substructures
Chaichoompu, Kridsadakorn ULg; Fouladi, Ramouna ULg; Wangkumhang, Pongsakorn et al

Poster (2014, April 29)

Objective To identify and differentiate between subpopulations using a rich set of genetic markers, as using reduced sets of genetic markers for these purposes can become challenging, especially when ... [more ▼]

Objective To identify and differentiate between subpopulations using a rich set of genetic markers, as using reduced sets of genetic markers for these purposes can become challenging, especially when similar geographic regions are involved or when spurious patterns are likely to exist. Method Single Nucleotide Polymorphisms (SNPs) are commonly used to capture variations between populations and often genome-wide SNP data are pruned based on linkage disequilibrium (LD) patterns. Notably, haplotype composition and the pattern of LD between markers may vary between larger populations but may also play a role within more confined geographic regions. Indeed, knowledge about haplotypes in unrelated individuals can reveal useful information about genetic ancestry. Here, we use iterative pruning principal component analysis (ipPCA) [1] to identify and characterize subpopulations in an unsupervised way. As input data, either pruned genome-wide SNP data are used (using PLINK 1.9 with the "indep-pairwise" option, window size = 100k, r2 < 0.25) or multilocus haplotype information derived from the genome-wide SNP panel (using BEAGLE 3.3.2 to infer haplotype). These approaches are applied to real-life data from 992 Thai individuals [2]. Result Preliminary results indicate that ipPCA applied to pruned SNP data or ipPCA that explicitly uses multilocus information (haplotypes) give complementary information about population substructure for geographically confined populations such as the Thai samples in this study. Both methods address different aspects of population structure. Detailed simulation studies are needed to identify the optimal scenarios for haplotype-based ipPCA. Conclusion In this work, we propose to combine an LD-based haplotype encoding scheme with the ipPCA machinery to retrieve fine population substructures. Despite the complexities that are associated with haplotype inference, added value can be obtained when the LD structure between SNPs is exploited in the search for relevant population strata. References 1. Intarapanich, A., et al., Iterative pruning PCA improves resolution of highly structured populations. BMC Bioinformatics, 2009. 10: p. 382. 2. Wangkumhang, P., et al., Insight into the peopling of Mainland Southeast Asia from Thai population genetic structure. PLoS One, 2013. 8(11): p. e79522. [less ▲]

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See detailApplication of multi-scale variography for inferring the spatial variability of the hydraulic conductivity of a sandy aquifer
Rogiers, Bart; Vienken, Thomas; Gedeon, M et al

Poster (2014, April 28)

In the framework of the disposal of short-lived low- and intermediate-level radioactive waste in a near-surface disposal facility in Dessel (Belgium), extensive characterization of the hydraulic ... [more ▼]

In the framework of the disposal of short-lived low- and intermediate-level radioactive waste in a near-surface disposal facility in Dessel (Belgium), extensive characterization of the hydraulic conductivity (K) in the shallow Neogene aquifer has been performed at a regional scale. In the last few years the small-scale heterogeneity has been additionally characterized by outcrop analogue, hydraulic direct push, and borehole core air permeameter studies. The gathered data now include a) more than 350 hydraulic conductivity measurements on samples from 8 cored boreholes, mostly reaching depths of 50 m and data at 2 m intervals, b) more than 5000 air permeability measurements on the same borehole cores, c) more than 250 cone penetration tests (CPTs) with depths down to 40 m and data at 2 cm intervals, d) over 100 dissipation tests performed during the CPT campaigns, e) 17 direct push injections loggings, 6 hydraulic profiling tool logs, and 6 direct push slug tests, f) several hundreds of air permeability measurements on outcrop analogues of the aquifer sediments, and g) numerous grain size analyses. The current study aims to quantify the heterogeneity of K from the centimetre- to the kilometre-scale and to check the compatibility of the spatial variability revealed by the different datasets. This is achieved through gathering all K values (either direct measurements, calibrated relative K values, or K estimates from secondary data), and the use of variography to quantify spatial variability in terms of two-points geostatistics. The results are discussed, and the main differences between the different data sources are explained. In a final step, different multi-scale variogram models are proposed for capturing the main characteristics of multi-scale variability within the shallow Neogene aquifer in Belgium. [less ▲]

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See detailCharcoal records reveal past occurrences of perturbations in the forests of the Kisangani region(RDC): vegetation history of the semi-deciduous rainforest
Tshibamba Mukendi, John; Hubau, Wannes; Morin-Rivat, Julie ULg et al

Poster (2014, April 28)

Introduction. Past disturbances have modified the structure and the floristic composition of Central African rainforests. These perturbations represent a driving force for forest dynamics and they are ... [more ▼]

Introduction. Past disturbances have modified the structure and the floristic composition of Central African rainforests. These perturbations represent a driving force for forest dynamics and they are presumably at the origin of present-day forest mosaics. Fire is a prominent forest disturbance, leaving behind charcoal as a witness of past forest dynamics. The question arises whether quantification, dating and botanical identification of ancient charcoal fragments found in soil layers (pedoanthracology) allows a detailed reconstruction of forest history, including the possible occurrence of past disturbances. Material & methods. We organized pedoanthracological excavations in 6 regrowth sites and 48 sites of primary forests of Yangambi, Yoko, Masako and Kole in the Kisangani (RDC). We performed a detailed sampling in different vegetation types of a semi -deciduous rainforest (Yoko). Charcoal sampling was conducted in pit intervals of 10 cm. The charcoal was quantified whereby pottery fragments were also registered. A selection of charcoal fragments has been dated through AMS 14C measurement. Floristic identifications were conducted using. former protocols based on wood anatomy, which is largely preserved after charcoalification. Results. Charcoal was found in most pit intervals. The anthracomass in the soil of regrowth forests (secondary forests) is much higher than in the primary forest: 27,59 mg/kg for secondary forests et 2,53 mg/kg for primary forests. The specific soil anthracomass of the primary forest of the Yoko reserve is higher (7,7 mg/kg) than in Yangambi (1,9 mg/kg) , Masako (1,7 mg/kg) and Kole (0,8 mg/kg). No systematic differences have been found between soil charcoal content of the different forest type representing different forest histories. Gilbertiodendron dewevrei (De Wild.) J. Leonard forests showed surprisingly a higher concentration of soil charcoal. Discussion. Forest disturbances in the Kisangani region appear to be more recent than those in the Mayombe forest in Western RDC ( 3000-2000 calBP (Hubau, 2013)) and those of the Cameroon forest (2300-1300 calBP) (Morin-Rivat, J et al., 2014). Stratified charcoal conserved in the soil is a useful indicator of past forest disturbances. [less ▲]

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See detailAnaerobic methane oxidation in two tropical freshwater systems
Roland, Fleur ULg; Darchambeau, François ULg; Crowe, SA et al

Poster (2014, April 27)

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See detailDrivers on carbon dioxide emissions from the Scheldt river basin
Gypens, N; Passy, P; Garnier, J et al

Poster (2014, April 27)

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See detailProduction of dissolved organic matter by phytoplankton and its uptake by heterotrophic prokaryotes in large tropical lakes
Morana, C; Sarmento, H; Descy, J-P et al

Poster (2014, April 27)

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See detailTemporal variability and spatial distribution of suspended matter and organic C pool in the Zambezi River
Teodoru, C; Bouillon, S; Borges, Alberto ULg et al

Poster (2014, April 27)

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See detailEutrophication counteracts ocean acidification effects on DMS emissions
Gypens, N; Borges, Alberto ULg

Poster (2014, April 27)

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See detailInorganic and organic carbon spatial variability in the Congo River during high waters (December 2013)
Borges, Alberto ULg; Bouillon, S; Teodoru, C et al

Poster (2014, April 27)

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See detailCarbon cycling in a large, meromictic tropical lake (Lake Kivu, East Africa): insights from seasonal monitoring of biogeochemical depth profiles
Morana, C; Darchambeau, F; Muvundja, F et al

Poster (2014, April 27)

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See detailCirculating microRNAs as diagnostic markers in breast cancer patients and their deregulation under neoadjuvant chemotherapy
FRERES, Pierre ULg

Poster (2014, April 24)

Breast cancer is the leading cause of death by cancer among women and there is an urgent need to improve its diagnosis and prognosis. MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression ... [more ▼]

Breast cancer is the leading cause of death by cancer among women and there is an urgent need to improve its diagnosis and prognosis. MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression and many have been implicated in breast cancer. In this article, we focus on circulating miRNAs as biomarkers for breast cancers and we describe the deregulation of their expression during neoadjuvant chemotherapy (NAC). [less ▲]

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See detailSpatio-Temporal Analysis of Equatorial Ionospheric Scintillations in the Frame of Absolute GNSS Positioning Algorithms
Lonchay, Matthieu ULg; Cornet, Yves ULg; Aquino, Marcio et al

Poster (2014, April 23)

The ionosphere has always been a major limitation for GNSS positioning applications. Free electrons in the ionosphere perturb the propagation of GNSS radio signals involving both refraction and ... [more ▼]

The ionosphere has always been a major limitation for GNSS positioning applications. Free electrons in the ionosphere perturb the propagation of GNSS radio signals involving both refraction and diffraction effects. The ionospheric refraction mainly results in a modification of the propagation speed of the GNSS electromagnetic signals, inducing an error (propagation delay or phase advance depending on the observable) in GNSS measurements. In the frame of absolute positioning techniques, single-frequency algorithms usually exploit an ionospheric model to mitigate the ionospheric error while dual-frequency algorithms, such as the well-known Precise Point Positioning (PPP), take the benefit of the availability of two frequencies and the fact that the ionosphere is a dispersive medium to construct an ionosphere-free mathematical model. But these two strategies are not able to counteract the effect of the ionospheric diffraction which is due to small-scale irregularities in the free electron density. By scattering GNSS signals, these irregularities generate rapid fluctuations (scintillations) in the amplitude and phase of GNSS signals with critical consequences for GNSS applications: cycle slips, signal power fading, receiver loss of lock and poor resulting satellite geometry. The goal of our research is to develop a strategy to mitigate the effect of ionospheric scintillations on absolute GNSS positioning techniques, in particular the SPP (Standard Point Positioning) and the PPP (Precise Point Positioning). The strategy is based on the adjustment of the stochastic model. In order to construct the stochastic model (diagonal and non-diagonal elements) and study the correlation between observables, we adopted a “spatial” and an “empirical” approach. The spatial approach consists in a study of the spatial autocorrelation existing in scintillations effects on GNSS signals. The spatial autocorrelation is detected by using specific spatial analysis techniques applied on data from a network of ISMR (Ionospheric Scintillation Monitoring Receiver) stations located at equatorial and polar latitudes, where scintillations effects are most severe. The knowledge of how scintillation effects are spatially correlated is helpful for determining a coherent stochastic model. The empirical approach does not take into account the phenomenon spatiality and the locations of the measurements but only the observation data. Its objective is to determine the statistical correlation which exists between GNSS measurements during a scintillation event by using a moving filter applied on GNSS observation and scintillation data. The spatial approach exploits data and data locations while the empirical approach is based only the data itself. [less ▲]

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See detailStudies of the Domains II and III of Bacillus subtilis PBP4a in relation with the protein localization
Vanden Broeck, Arnaud ULg; Van Der Heiden, Edwige ULg; Sauvage, Eric ULg et al

Poster (2014, April 23)

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them have been ... [more ▼]

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them have been mutated in glutamine residues (Q). Both proteins (WT and Mut4KQ PBP4a) have been produced without signal peptide in E. coli and their sub-cellular localizations determined by measuring the DD-carboxypeptidase activities in the different compartments (cytoplasmic vs membrane attached proteins). In order to detect a possible influence of the PBP4a domain III in the localization of the protein, its encoding sequence has been cloned into pET-28b-BlaP, a vector allowing the production of WT BlaP β-lactamase or BlaP/DIII chimeric protein (with domain III inserted in a permissive loop of BlaP). The nitrocefin hydrolysis activities of BlaP or BlaP/DIII have been measured in the different cellular compartments. [less ▲]

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See detailRéaction immédiate à l'alcool
LIBON, Florence ULg; DEZFOULIAN, Bita ULg; NIKKELS, Arjen ULg

Poster (2014, April 17)

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See detailPhylogenetic study of Aedes albopictus and Aedes koreicus (Diptera, Culicidae) origin, two invasive mosquito species in Belgium
Raharimalala, Fara Nantenaina ULg; Boukraa, Slimane ULg; Bawin, Thomas ULg et al

Poster (2014, April 10)

The main ways of the introduction of invasive mosquito species in Europe were greatly facilitated by the importation of used tires and plants from the South of China (Dracaena sanderiana, called "Lucky ... [more ▼]

The main ways of the introduction of invasive mosquito species in Europe were greatly facilitated by the importation of used tires and plants from the South of China (Dracaena sanderiana, called "Lucky bamboo"), which are a very good breeding sites of these mosquitoes (www.eidatlantique.eu). For Belgium, several species of exotic mosquitoes have already been listed such as Ochlerotatus japonicus japonicus, Aedes albopictus and A. koreicus. We have captured one male of A. albopictus in Belgium in July 2013 (Boukraa et al, 2013), and teams from other entomologist have been able to find several individuals in the months that have followed our discovery (personal communication). However, his presence was reported first in 2000 (Schaffner et al. 2004) but until 2012, no individuals have been found (Versteirt et al., 2013). We also could find larvae of A. koreicus in abundance. These discovery suggest that these two invasive species are being established in Belgium. Methods: Adult mosquitoes were collected by CO2-baited traps Mosquito Magnet Liberty Plus (MMLP) and immature stages by the dipping method from 8 sites of Belgium. Each individual was then determined morphologically. For A. albopictus and A. koreicus, reconfirmation by molecular method was performed with COI and ND5 mitochondrial primers and sequences were then aligned with those of mosquitoes available in databases by using BioEdit and Multialn softwares. The two gene sequences were concatenated to improve the reliability of the phylogenetic analysis and were carried out by using the Seaview software based on maximum likelihood (ML) methods. Trees were then constructed with the general time reversible (GTR) model, and branch supports were estimated by bootstrapping with 1000 replicates. Result: The aim of this work will focus on the study of the probable origin of these two species of Aedes, which are for the moment safe yet, but that might become dangerous in the event of massive outbreak. The knowledge of the probably origin of the two invasive mosquitoes allow take more protection against their way to enter in the country. [less ▲]

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See detailLo sviluppo socio-emotivo dei bambini adottati da coppie omosessuali in Europa
Messina, Roberta ULg; D'Amore, Salvatore ULg; J-Green, Robert

Poster (2014, April 10)

L’adozione da parte di coppie omosessuali, legale in 9 paesi europei, provoca molte reazioni nell’opinione pubblica. Il dibattito oppone il “diritto al bambino”, reclamato dagli omosessuali, al “diritto ... [more ▼]

L’adozione da parte di coppie omosessuali, legale in 9 paesi europei, provoca molte reazioni nell’opinione pubblica. Il dibattito oppone il “diritto al bambino”, reclamato dagli omosessuali, al “diritto del bambino” di crescere al sicuro da ogni forma di stigmatizzazione. La presente ricerca, pionieristica nel contesto europeo, mira ad approfondire lo sviluppo socio-emotivo dei bambini adottati da coppie dello stesso sesso. L’obiettivo è rispondere all’interrogativo soggiacente al dibattito sociale: “inserire un bambino, già segnato da un abbandono, in una famiglia che rappresenta un nuovo “cantiere sociale” può comportare delle ripercussioni sul suo benessere psicologico?”. Recruteremo circa 200 famiglie adottive, metà omogenitoriali e metà eterogenitoriali; per ogni famiglia, i dati saranno raccolti tramite tre fonti di informazione: i genitori, il bambino e un suo insegnante. La ricerca, condotta inizialmente in Olanda, Belgio, Spagna e Inghilterra, sarà poi diffusa negli altri paesi europei. [less ▲]

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See detailSame-sex parenting is legal in Belgium but some heterosexuals support it and others don't: why?
Scali, Thérèse ULg; D'Amore, Salvatore ULg; Green, Robert-Jay

Poster (2014, April 10)

Gays and Lesbians represent between 7 and 10% of the Belgian population, this means 1 million of citizens. Notwithstanding the large number of civil rights granted to homosexuals and the anti ... [more ▼]

Gays and Lesbians represent between 7 and 10% of the Belgian population, this means 1 million of citizens. Notwithstanding the large number of civil rights granted to homosexuals and the anti-discrimination laws, the issue of social acceptance of same-sex couples and families remains. However, the attitude of the population (support vs. rejection and discrimination) and the marginalization of a minority influence not only their quality of life but also their couple and parenting skills and the development of their children (Armesto, 2002; Goldberg, 2010). Results show that attitudes towards same-sex parenting can be explained by four categories of arguments: arguments regarding children well-beeing, religious and political arguments, contact and experiential arguments and traditional arguments. [less ▲]

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See detailHabitat use of a population of bottlenose dolphins, Tursiops truncatus gephyreus, analyzed by means of Kernel Density Estimation (KDE) method
Cransveld, Alice ULg; Denoël, Mathieu ULg; Das, Krishna ULg et al

Poster (2014, April 09)

The San Antonio Bay (SAB), in Patagonia, Argentina, harbors a resident population of bottlenose dolphins (Tursiops truncatus gephyreus). It seems a privileged area to give birth and nurse calves. In the ... [more ▼]

The San Antonio Bay (SAB), in Patagonia, Argentina, harbors a resident population of bottlenose dolphins (Tursiops truncatus gephyreus). It seems a privileged area to give birth and nurse calves. In the context of declining populations worldwide and more particularly in South America, preserving the SAB population takes a considerable significance. Yet the SAB is facing human population growth and touristic development, which represent potential threats for the dolphin population, especially dolphin-watching activities. In this context, the aim of this study was to understand the bottlenose dolphin’s habitat use within the bay, and to consider how this information could be used in prospective management strategies. Particularly, we aimed at using the Kernel Density Estimation (KDE) method to map the intensity of space use for essential behavioural patterns. To this end, we collected behavioural information on dolphins during 25 boat-based surveys in the bay in 2011. The habitat use of the bay was heterogeneous: some areas were more intensely used than others. Dolphins spent most of their time traveling and diving. Variables associated to resting behaviours, e.g. school size and depth, indicated that the SAB would be a safer place compared to other known residency areas, confirming its suitability for conservation purposes. KDE analyses showed that behaviours are not evenly distributed inside the bay. Bottlenose dolphins being more sensitive to anthropogenic disturbances while engaged in resting or socializing behaviours, it is crucial to locate these behaviours. In SAB, the KDE shows that resting and socializing areas are located in the Northern part of the bay, indicating that it should constitute a priority protected area in potential future management strategies. Furthermore, our results show that the KDE method is an appropriate and advantageous tool when determining critical habitats, worth being more widely used. [less ▲]

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See detailProbing the conformational changes during desolvation of ions using orthogonal mobility methods (CE-IMS)
Far, Johann ULg; Kune, Christopher ULg; Delvaux, Cédric ULg et al

Poster (2014, April 08)

The transfer of ions from the solution to the gas phase is a critical step to produce « native species ». Coming from a highly solvating medium, ionic species will tend to find a new equilibrium ... [more ▼]

The transfer of ions from the solution to the gas phase is a critical step to produce « native species ». Coming from a highly solvating medium, ionic species will tend to find a new equilibrium conformation in the gas phase. The pathway to reach the thermodynamically stable conformation involves crossing potential barriers of different heights. When these barriers are too high compared to the internal energy of the ions, it will result in “partial memories” (as structural preservation) of the conformation in solution. In order to evaluate the effect of the solvent evaporation and of the various collision processes encountered by the ions in the mass spectrometer. The strategy consists in comparing in a single experiment the shape of the ions in solution and in the gas phase. Data are obtained by coupling capillary electrophoresis with Ion Mobility Mass Spectrometry. Drift times in solution and in the gas phase are directly compared. Deviations from their correlation points out changes in folding upon desolvatation. Preliminary results show that among peptides issued from tryptic digest of BSA some of them clearly change their conformation during desolvatation. This work intends to evaluate the extent of conformational “memory” of the ions of different nature for best experimental condition allowing “native mass spectrometry”. [less ▲]

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See detailMultitasking capacities in persons diagnosed with schizophrenia: A preliminary examination of their neurocognitive underpinnings and ability to predict real world functioning
Laloyaux, Julien ULg; Van der Linden, Martial ULg; Levaux, Marie-Noëlle ULg et al

Poster (2014, April 08)

Background: Difficulties in everyday life activities are core features of persons diagnosed with schizophrenia. Moreover, patients seem to demonstrate particular difficulties during complex and ... [more ▼]

Background: Difficulties in everyday life activities are core features of persons diagnosed with schizophrenia. Moreover, patients seem to demonstrate particular difficulties during complex and multitasking activities, such as cooking a meal (Semkovska et al., 2004). Multitasking refers to activities where the person has to: carry out and alternate between different tasks that vary in terms of priority, difficulty and duration; define the tasks’ targets; and where the person is faced with unexpected problems during the realization of these tasks (Burgess, 2000). However, at present, patients’ multitasking capacities have not been adequately examined in the literature due to an absence of suitable assessment strategies. We thus recently developed a computerized real-life activity task designed to take into account the complex and multitasking nature of certain everyday life activities where participants are required to prepare a room for a meeting – the Computerized Meeting Preparation Task (CMPT) Methods: Twenty-one individuals diagnosed with schizophrenia and 20 matched healthy controls completed the CMPT. During the CMPT, participants found themselves in a virtual room that they had to prepare for a meeting while respecting a list of instructions (the placement of the guests, the needed objects, the desired drinks, etc.). Patients were also evaluated with an extensive cognitive battery (assessing executive functions, attention, processing speed and memory), measures of symptomatology and real world functioning. To examine the ecological validity of the CMPT, 14 others patients were recruited and were given the computerized version and a real version of the meeting preparation task. Results: Results demonstrated that performance on the CMPT significantly differentiated patients and healthy controls for the total time to complete task, planning efficiency, and the respect of the instructions. Moreover, these variables were significantly correlated with executive functioning (i.e. cognitive flexibility and planning), suggesting the major implication of these cognitive processes in multitasking activities. Performance on the CMPT also significantly predicted up to 50% of real world functioning. Finally, performances on the computerized version and the real version of the meeting preparation task were highly correlated, suggesting good ecological validity. Discussion: In this study, we created a novel task involving the multitasking nature of real world activities. The results demonstrated that this approach provides a good indication of the real world functioning in patients diagnosed with schizophrenia. Moreover, results suggest a particular implication of executive functioning in multitasking activities. These findings suggest the importance of evaluating multitasking capacities in patients diagnosed with schizophrenia in order to predict real world functioning. [less ▲]

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See detailEfficacité des interven8ons langagières basées sur la guidance parentale : qu’en est- il pour les popula8ons de faible statut socio-économique (SSE)?
Maillart, Christelle ULg; Martinez Perez, Trecy ULg; Jidovtseff, Boris ULg et al

Poster (2014, April 07)

Le développement langagier de l’enfant est fortement influencé par le statut socio-économique (SSE). Dès le plus jeune âge, les enfants issus de milieux défavorisés présentent un retard langagier ... [more ▼]

Le développement langagier de l’enfant est fortement influencé par le statut socio-économique (SSE). Dès le plus jeune âge, les enfants issus de milieux défavorisés présentent un retard langagier objectivable (Hoff, 2012). La qualité et la quantité du langage adressé à l’enfant sont des variables médiatrices dans l’impact du SSE (Hoff, 2003). Une méta-analyse a mis en évidence l’efficacité des interventions langagières ciblées sur l’enrichissement du langage adressé à l’enfant (Robert & Kaiser, 2011). Toutefois, les études d’intervention ont principalement porté sur des enfants issus de SSE moyen : il est difficile d’en généraliser la portée aux populations plus défavorisées. Cette étude randomisée contrôlée évalue l’efficacité d’une intervention ciblée sur le langage adressé à l’enfant dans des populations de faible SSE. Participants Quatre-vingt-seize enfants âgés de 18 à 30 mois et de faible SSE ont été recrutés au sein de 10 consultations enfants organisées par l’Office de la Naissance et de l’Enfance, en Belgique. Dans 5 de ces 10 consultations, une intervention portant sur la stimulation langagière a été proposée. Dans les 5 autres, une intervention en psychomotricité a été mise en place. Les deux interventions se déroulent en groupe (une dizaine d’enfants et leur parent) et sont appariées en termes de fréquence (1 heure par semaine) et de durée (15 semaines). Une évaluation individuelle a été proposée avant l’intervention (T1), après l’intervention (T2) et 6 mois après la fin de l’intervention (T3). Chaque testing évaluait les développements cognitif, langagier et psychomoteur de l’enfant et comportait une interaction filmée parent-enfant en situation de jeu afin d’analyser le langage adressé à l’enfant et les comportements parentaux. Principaux résultats Les résultats mettent en évidence l’efficacité et la spécificité de l’intervention langagière proposée, tant au niveau du langage des enfants que des modifications du comportement parental. [less ▲]

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See detailDevelopmental Invariance in Implicit Sequence Learning
Lejeune, Caroline ULg; Wansard, Murielle ULg; Meulemans, Thierry ULg

Poster (2014, April 05)

This study was intended to test the age invariance hypothesis on implicit learning abilities using the serial reaction time paradigm and focusing on the comparison of second-order conditional (SOC ... [more ▼]

This study was intended to test the age invariance hypothesis on implicit learning abilities using the serial reaction time paradigm and focusing on the comparison of second-order conditional (SOC) sequences of two different lengths (8 and 12 elements). A total of 128 participants from 4 age groups (4 years, 7 years, 10 years, and adults) were tested. The results showed significant and similar learning effects in 4-, 7-, and 10-year-old children, as well as adults. The learning effect was more pronounced for the 8-element sequence than for the 12-element sequence for all age groups, suggesting that the shorter sequence was better learned than the longer one. In addition, the degree of explicit sequence awareness was comparable between age groups and sequence lengths. These results, showing that 4-year-old children are able to learn 8- and 12-element-long SOC sequences as well as adults, provide further support for the hypothesis that implicit learning abilities are developmentally invariant. [less ▲]

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See detailNo simian Plasmodium detected in populations living in the equatorial rainy forest of the Democratic Republic of Congo
Mvumbi makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

Poster (2014, April 03)

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four ... [more ▼]

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four species of Plasmodium (P. falciparum, P. vivax, P. malariae and P. ovale) were responsible for the disease in humans. But quite recently, a fifth species, Plasmodium Knowlesi, has been identified as naturally infecting humans. Indeed, known for decades as naturally parasitizing the monkey Macaca fascicularis, P. knowlesi has long been confused, in terms of its evolutionary stage, with P. malariae or P. falciparum, which it resembles morphologically and it was not possible to properly differentiate them until the advent of molecular biology. To date, P. Knowlesi has only been identified in Southeast Asia and a similar phenomenon of natural transmission of simian plasmodium to humans has not been reported elsewhere. We therefore conducted this study to investigate the possible transmission of simian plasmodium to humans in populations living near the rainforest of the Democratic Republic of Congo (DRC) where several species of primates lives. Methods & Materials Three villages (Wenji-Secli, Bongonde, and Bolenge) in the Province of Ecuador (North-eastern DRC) were selected because of their geographical location. Blood samples spotted on filter paper were collected from 100 people randomly taken in each village. Two successive RT- PCR were performed. A first one using a single probe able to diagnose all plasmodium spp. and a second using four species-specific probes for the diagnosis of the four conventional human plasmodium species. Positivity in the first RT- PCR with negativity in the second RT- PCR would suggest the presence of plasmodium species other than the four conventional. Results P. falciparum was correctly identified in 44.6 % of samples. No other species of human plasmodium or not has been identified. Conclusion This preliminary study did not detect the presence of simian plasmodium in human populations living in the rainforest of the DRC. Studies with larger samples and with more advanced techniques should still be conducted. Keywords: Malaria, simian plasmodium, DR Congo [less ▲]

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See detailBiological control of aphids in wheat and vegetable crops : a multi-approach case study in Shandong province (China)
Chevalier Mendes Lopes, Thomas ULg; Bosquée, Emilie ULg; Chen, Julian et al

Poster (2014, April 02)

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches ... [more ▼]

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches were tested: (1) wheat/oilseed rape and wheat/pea associations, (2) potatoes/peas association, and (3) E-β-farnesene (aphid alarm pheromone) releasers in squashes under plastic tunnels. Aphids and aphidophagous beneficials were monitored by observations on plants. Wheat associations and E-β-farnesene reduced aphid infestations compared with control treatments, while no effect was observed in the potatoes/peas association. Moreover, aphidophagous beneficials were significantly more abundant in the three kinds of associations compared with control treatments. Few individuals were observed on squashes. Additionally, adult aphidophagous beneficials were monitored using yellow pan traps. The parasitoid species Aphidius gifuensis (Ashmead) was prevalent, while Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) were the most abundant species among predators. Globally, wheat associations were more efficient to reduce aphid infestations and attract aphidophagous beneficials to promote biological control. The trapping of natural enemies allowed to assess the diversity and abundance of aphidophagous species in this region. This study shows a beneficial effect of crop associations and E-β-farnesene on the control of aphid populations and contributes to assess the potentialities of these alternative methods to the use of insecticides. [less ▲]

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See detailA pheromone-based formulation against phytophagous pests
Fassotte, Bérénice ULg; Vandereycken, Axel ULg; Heuskin, Stéphanie ULg et al

Poster (2014, April 02)

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or ... [more ▼]

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or indirect biocide activity is one promising alternative option. Our researches focus on the identification of appropriate formulations releasing volatile organic compounds that are attractive for natural enemies of insect pests. However, the elaboration of slow-release devices that ensure stable and controlled release of active volatile compounds is quite challenging. Here, we developed a formulation based on E-β-farnesene and (–)-β-caryophyllene, these two semiochemicals having strong attractive potential on aphid natural enemies including ladybeetles and hoverflies. Both compounds were encapsulated together in alginate gel beads. The blend efficiency is currently being evaluated through laboratory and field assays. [less ▲]

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See detailStudy of sex ratio and morphotypes of the Multicoloured Asian Ladybird, Harmonia axyridis Pallas in Belgian corn
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Barsics, Fanny ULg et al

Poster (2014, April 02)

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian ... [more ▼]

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian Ladybird, which is considered as an invasive species in Europe since its introduction in the 80’s. Harmonia axyridis has four major morphotypes: 2 melanic forms, conspicua and spectabilis; and 2 non-melanic, succinea and axyridis. In Belgium, only the three first are observed. Literature reports variations in morphotype frequencies, across native and introduced strains. These variations seem to be linked to climate (geographical and seasonal variation), with non-melanic forms being the most abundant in hot and arid climate. This study focused on the variation of morphotype abundance in H. axyridis according to season, field and gender. Adult ladybirds were caught from mid-July to mid-October 2012 into 5 corn fields. The proportions of observed morphotypes were compared according to the sampling season. The abundance of morphotypes was not significantly different through the fields; the observed ratios were 81.6%, 15% and 3.4% for succinea, spectabilis and conspicua respectively. Succinea was the most observed morphotype (χ2=755.8; P<0.001). The total number of collected males and females was the same with ratios of 48.2% and 51.8%: (χ2=0.93; P=0.33). The sex ratio was also not significantly different for each morphotype. There was no evolution of melanic and non-melanic form ratio according to the season, with 25.6% of melanic form in summer and 20.6% in autumn (χ2=1.22; P=0.27). Our results seem to show that morphotype adaptation according to the climate is not observed for Belgian ladybirds. We discuss these results according to all environmental data available. [less ▲]

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See detailConservatoire entomologique de Gembloux Agro-Bio Tech: les coccinelles de Wallonie et de Bruxelles de 2001 à 2009
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Durieux, Delphine ULg et al

Poster (2014, April 02)

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009 ... [more ▼]

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009. Cette collection est essentiellement constituée par les récoltes des étudiants de première Bachelier de Gembloux Agro-Bio Tech. A travers ce recensement, nous avons étudié l’évolution des espèces récoltées au sein de cette famille en termes d’effectif relatif et de richesse spécifique. Notre étude s’est focalisée sur les données issues de Wallonie et de la Région de Bruxelles-Capitale, trop peu de collectes ayant été réalisées en Flandre. Parmi les individus identifiés, 27 espèces ont été observées, dont 21 appartiennent à la sous-famille des Coccinellinae, 2 à celle des Epilachninae et 4 à celle des Chilocorinae. La plupart des espèces sont communes à la Belgique. Cependant, Harmonia axyridis (Pallas 1773), espèce originaire du sud-est de l’Asie, a été introduite sur le territoire belge en 1997. Cette coccinelle exotique fait son apparition dans les récoltes de 2002 et son effectif ne cesse d’augmenter au fil des années prospectées. En parallèle à cette augmentation, une diminution de la richesse spécifique, ainsi que de l’effectif relatif d’Adalia bipunctata (L. 1758), de Propylea quatuordecimpunctata (L. 1758) et de Psyllobora vigintiduopunctata (L. 1758), est observée. [less ▲]

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See detailWill top-down interactions be affacted by climate change?
Boullis, Antoine ULg; Verheggen, François ULg; Francis, Frédéric ULg

Poster (2014, April 02)

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb ... [more ▼]

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb in industrialized areas. According to forecasting models, these concentrations are expected to double by the end of the 21st century. To date, impacts of elevated greenhouse gases have been extensively studied in plant-insect interactions, whereas interactions between herbivores and their natural enemies have been neglected. Because most interactions between herbivorous insects and their natural enemies are orchestrated by natural molecules produced as semiochemicals, we hypothesize that modification in atmospheric gas composition will alter chemical communication in insects, and affect the performance of natural enemies to locate their prey. In this project, we shall attempt to analyze the effect of increase in greenhouse gas concentrations on insect communication, particularly in top-down interactions. The study model will consist in the interactions between the pea aphids (Acyrthosiphon pisum (Harris)) and their natural enemies Harmonia axyridis (Pallas) and Episyrphus balteatus (DeGeer). Under elevated CO2 and/or O3 concentrations, we will quantify the production of several aphid semiochemicals that are currently used as kairomones by natural enemies, including the aphid alarm pheromone E-β-farnesene and honeydew volatile compounds. Olfactometry assays will then be set up to examine the ability of natural enemies to locate their prey in the predicted atmospheric conditions of the end of the 21st century. Finally, using an Ecotron structure to be built in Gembloux Agro-Bio Tech, we will simulate a future ecosystem with the possibility to control additional biotic and abiotic parameters. [less ▲]

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See detailNeutralizing properties of peptides derived from CXCR4 extracellular loops towards CXCL12 bidnig and HIV-1 infection
Szpakowska, Martyna ULg; Fievez, Virginie; Counson, Manuel et al

Poster (2014, April)

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See detailTRAPPIST monitoring of comet C/2012 F6 (Lemmon)
Opitom, Cyrielle ULg; Jehin, Emmanuel ULg; Manfroid, Jean ULg et al

Poster (2014, April)

C/2012 F6 (Lemmon) is a long period comet discovered by the Mount Lemmon Survey on 2012 March 23 at 5 AU from the sun. C/2012 F6 (Lemmon) reached perihelion on March 23, 2013 at 0.73 AU from the sun. In ... [more ▼]

C/2012 F6 (Lemmon) is a long period comet discovered by the Mount Lemmon Survey on 2012 March 23 at 5 AU from the sun. C/2012 F6 (Lemmon) reached perihelion on March 23, 2013 at 0.73 AU from the sun. In December 2012 the comet was unexpectedly bright, allowing us to make an extensive monitoring during several months with both broadband and narrowband filters to follow the evolution of the comet chemical composition. The monitoring was made with TRAPPIST robotic telescope installed at La Silla observatory [1]. TRAPPIST is a 60-cm telescope dedicated to the study of exoplanets and small bodies in the solar system. The telescope is equipped with a 2Kx2K FLI Proline CCD camera very sensitive in the blue and the red. A set of narrowband cometary filters designed by the NASA for the Hale-Bopp Observing Campaign [2] is permanently mounted on the telescope along with classic Johnson-Cousins B, V, Rc, and Ic filters. We observed the comet from December 11, 2012 to March 4, 2013 (pre-perihelion) and from April 29, 2013 to June 11, 2013 (post-perihelion). At least 2 or 3 observing runs per week were programmed during this period. We collected 1358 images on 52 nights. In January and February the comet visibility allowed us to make several long runs and to detect the comet rotational variability. From the comet images in narrowband filters we studied the gaseous coma chemical composition and activity by deriving OH, NH, CN, C2 and C3 production rates using a classical Haser model [3]. The production and properties of the dust component were studied through the observation of C/2012 F6 (Lemmon) with narrowband continuum filters at 344.2 nm (UC), 444.9 nm (BC), 525.7 nm (GC) and 713.0 nm (RC). We used A(θ)fρ [4] parameter as a proxy for the dust production. [less ▲]

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See detailEcomorphology of the vertebral column: preliminary study
Gillet, Amandine; Ninane, Catherine; Zaeytydt, Esther et al

Poster (2014, April)

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See detailEpigenetic mechanisms underlying long-term programming effects of prenatal protein undernutrition by albumen removal in the chicken
Willems, E.; Guerrero-Bosagna, C.; Buyse, J. et al

Poster (2014, April)

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See detailvCCL2, the third agonist ligand for the -arrestin-biased chemokine receptor CXCR7
Szpakowska, Martyna ULg; Derj, Anouar ULg; Counson, Manuel et al

Poster (2014, April)

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See detailThe Neural Correlates of Re-cancellation Behaviors in Unilateral Neglect: A Neuropsychological Approach
Wansard, Murielle ULg; Bastin, Christine ULg; Meulemans, Thierry ULg

Poster (2014, April)

The present study focused on re-cancellation behaviors in unilateral neglect (i.e., the tendency to search repeatedly items located on the right side in visual search tasks), and used a neuropsychological ... [more ▼]

The present study focused on re-cancellation behaviors in unilateral neglect (i.e., the tendency to search repeatedly items located on the right side in visual search tasks), and used a neuropsychological approach to identify the cerebral correlates of this deficit. Fourteen patients suffering from left neglect and 14 elderly age-matched controls performed a cancellation task without visual feedback. Neglect patients cancelled fewer targets than controls, and re-cancelled an abnormally high number of targets. Lesion maps were used to compare the location of brain damage in neglect patients with the highest versus the lowest percentage of re-cancellations. Anatomical data revealed that the right insula is commonly damaged in 5 out of 6 patients with the highest re-cancellation percentage, but is spared in the subgroup of patients with the lowest re-cancellation percentage. These results suggest that damage to the right insula may contribute to pathological visual search in spatial neglect, possibly by reducing interaction between the ventral and dorsal attention network (the latter being more directly involved in spatial processes). [less ▲]

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See detailIn vitro culture of seal muscle-derived satellite cells
Freichels, Astrid ULg; Baise, Etienne ULg; Jauniaux, Thierry ULg et al

Poster (2014, April)

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See detailPolyplexes Targeting Angiogenesis in Cancer
Frère, Antoine ULg; Peixoto, Paul ULg; Kawalec, Michal et al

Poster (2014, April)

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See detailFLUSHING PORCINE DCD LIVERS WITH CYCLO-DEXTRIN COMPLEXED CURCUMIN DOES NOT REDUCE ISCHEMIA REPERFUSION INJURY
MEURISSE, Nicolas ULg; PARKINNEN, J; CEULEMANS, L et al

Poster (2014, April)

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See detailChemical analyses of the seeds from Prunella vulgaris: A chemotaxonomic approach
Paul, Aman ULg; Frederich, Michel ULg; Cieckiewicz, Ewa ULg et al

Poster (2014, April)

Common self-heal (Prunella vulgaris) plants are traditionally sown along the border of crops to enhance the biodiversity. Besides enhancing the biodiversity, they can also be a source of interesting ... [more ▼]

Common self-heal (Prunella vulgaris) plants are traditionally sown along the border of crops to enhance the biodiversity. Besides enhancing the biodiversity, they can also be a source of interesting compounds which could be important for food, pharmaceutical and cosmetic industries. The seeds of Common Yarrow were investigated for proteins, fatty acid compositions and polyphenolic compounds. The protein content was analyzed according to Dumas method, the extraction of oil was done using a cold extraction technique employing 2:1 chloroform/methanol as solvent, the fatty acid composition was determined using the gas chromatography and the amount of polyphenolic compounds were estimated using the method as described in European Pharmacopoeia, 8th edition. Common self-heal seeds can be of great commercial importance. [less ▲]

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See detailEvaluation of the relationships between characteristics of the vertebral column of different cetaceans and their ecology: A preliminary study.
Gillet, Amandine ULg; Ninane, Catherine ULg; Remy, François ULg et al

Poster (2014, April)

Ecomorphology is the study of the relationships between functional design and the environment. One of its aims is to understand how the environmental factors can constraint the performance of an organism ... [more ▼]

Ecomorphology is the study of the relationships between functional design and the environment. One of its aims is to understand how the environmental factors can constraint the performance of an organism or act on its phenotype. Different studies have already showed in different cetaceans that the number and shape of vertebrae can reflect the stiffness of the body and consequently can impact their swimming mode. The aim of this preliminary study is to establish relationships between characteristics of the vertebral column of different cetaceans and their ecology. To this purpose, we have studied meristic and morphometric data on the vertebrae (centrum length, height and width, neural and haemal spine height and the transverse process length) of different species of mysticetes and odontocetes coming from the Aquarium-Museum of Liège and Royal Institute of Natural Sciences of Bruxelles. Preliminary results showed the distinction of three morphotypes: firstly, the active, cruising, fast swimmers with rigid body, secondly, the maneuverers, slow swimmers with flexible body and thirdly, the steady swimmers. [less ▲]

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See detailA geochemical and petrological study of the Late Cretaceous banatites from the Apuseni Mountains, Romania
Vander Auwera, Jacqueline ULg; Berza, Tudor; Dupont, Alain et al

Poster (2014, April)

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See detailEARLY ALLOGRAFT DYSFUNCTION DECREASES PATIENT SURVIVAL AFTER LIVER TRANSPLANTATION
MEURISSE, Nicolas ULg; FIEUWS, S; JOCHMANS, I et al

Poster (2014, April)

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See detailModelling the Congo basin ecosystems with a dynamic vegetation model
Dury, Marie ULg; Hambuckers, Alain ULg; Trolliet, Franck ULg et al

Poster (2014, April)

The scarcity of field observations in some parts of the world makes difficult a deep understanding of some ecosystems such as humid tropical forests in Central Africa. Therefore, modelling tools are ... [more ▼]

The scarcity of field observations in some parts of the world makes difficult a deep understanding of some ecosystems such as humid tropical forests in Central Africa. Therefore, modelling tools are interesting alternatives to study those regions even if the lack of data often prevents sharp calibration and validation of the model projections. Dynamic vegetation models (DVMs) are process-based models that simulate shifts in potential vegetation and its associated biogeochemical and hydrological cycles in response to climate. Initially run at the global scale, DVMs can be run at any spatial scale provided that climate and soil data are available. In the framework of the BIOSERF project (“Sustainability of tropical forest biodiversity and services under climate and human pressure”), we use and adapt the CARAIB dynamic vegetation model (Dury et al., iForest - Biogeosciences and Forestry, 4:82-99, 2011) to study the Congo basin vegetation dynamics. The field campaigns have notably allowed the refinement of the vegetation representation from plant functional types (PFTs) to individual species through the collection of parameters such as the specific leaf area or the leaf C:N ratio of common tropical tree species and the location of their present-day occurrences from literature and available database. Here, we test the model ability to reproduce the present spatial and temporal variations of carbon stocks (e.g. biomass, soil carbon) and fluxes (e.g. gross and net primary productivities (GPP and NPP), net ecosystem production (NEP)) as well as the observed distribution of the studied species over the Congo basin. In the lack of abundant and long-term measurements, we compare model results with time series of remote sensing products (e.g. vegetation leaf area index (LAI), GPP and NPP). Several sensitivity tests are presented: we assess consecutively the impacts of the level at which the vegetation is simulated (PFTs or species), the spatial resolution and the initial land cover (potential or human-induced). First, we show simulations over the whole Congo basin at a 0.5◦ spatial resolution. Then, we present high-resolution simulations (1 km) carried out over different areas of the Congo basin, notably the DRC part of the WWF Lake Tele – Lake Tumba Landscape. Studied in the BIOSERF project, this area is characterized by a forest-savannah mosaic but also by swamp and flooded forest. In addition, forward transient projections of the model driven with the outputs of about thirty global cli- mate models (GCMs) from the new Coupled Model Intercomparison Project Phase 5 (CMIP5) will permit to outline the likely response of carbon pools to changing climate over the Congo basin during the 21th century. [less ▲]

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See detailMassive Depletion of Bovine Leukemia Virus Proviral Clones Located in Genomic Transcriptionally Active Sites During Primary Infection
Gillet, Nicolas ULg; geronimo, gutierrez; rodriguez, sabrina et al

Poster (2014, April)

Deltaretroviruses such as human T-lymphotropic virus type 1 (HTLV-1) and bovine leukemia virus (BLV) induce a persistent infection generally asymptomatic but can also lead to leukemia or lymphoma. These ... [more ▼]

Deltaretroviruses such as human T-lymphotropic virus type 1 (HTLV-1) and bovine leukemia virus (BLV) induce a persistent infection generally asymptomatic but can also lead to leukemia or lymphoma. These viruses replicate by infecting new lymphocytes (i.e. the infectious cycle) or via clonal expansion of the infected cells (mitotic cycle). The relative importance of these two cycles in viral replication varies during infection. The majority of infected clones are created early before the onset of an efficient immune response. Later on, the main replication route is mitotic expansion of pre-existing infected clones. Due to the paucity of available samples and for ethical reasons, only scarce data is available on early infection by HTLV-1. Therefore, we addressed this question in a comparative BLV model. We used high-throughput sequencing to map and quantify the insertion sites of the provirus in order to monitor the clonality of the BLV-infected cells population (i.e. the number of distinct clones and abundance of each clone). We found that BLV propagation shifts from cell neoinfection to clonal proliferation in about 2 months from inoculation. Initially, BLV proviral integration significantly favors transcribed regions of the genome. Negative selection then eliminates 97% of the clones detected at seroconversion and disfavors BLV-infected cells carrying a provirus located close to a promoter or a gene. Nevertheless, among the surviving proviruses, clone abundance positively correlates with proximity of the provirus to a transcribed region. Two opposite forces thus operate during primary infection and dictate the fate of long term clonal composition: (1) initial integration inside genes or promoters and (2) host negative selection disfavoring proviruses located next to transcribed regions. The result of this initial response will contribute to the proviral load set point value as clonal abundance will benefit from carrying a provirus in transcribed regions. [less ▲]

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See detailWeb-based application for Data INterpolation Empirical Orthogonal Functions (DINEOF) analysis
Tomazic, Igor ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2014, April)

DINEOF (Data INterpolating Empirical Orthogonal Functions) is a powerful tool based on EOF decomposition developed at the University of Liege/GHER for the reconstruction of missing data in satellite ... [more ▼]

DINEOF (Data INterpolating Empirical Orthogonal Functions) is a powerful tool based on EOF decomposition developed at the University of Liege/GHER for the reconstruction of missing data in satellite datasets, as well as for the reduction of noise and detection of outliers. DINEOF is openly available as a series of Fortran routines to be compiled by the user, and as binaries (that can be run directly without any compilation) both for Windows and Linux platforms. In order to facilitate the use of DINEOF and increase the number of interested users, we developed a web-based interface for DINEOF with the necessary parameters available to run high-quality DINEOF analysis. This includes choosing variable within selected dataset, defining a domain, time range, filtering criteria based on available variables in the dataset (e.g. quality flag, satellite zenith angle …) and defining necessary DINEOF parameters. Results, including reconstructed data and calculated EOF modes will be disseminated in NetCDF format using OpenDAP and WMS server allowing easy visualisation and analysis. First, we will include several satellite datasets of sea surface temperature and chlorophyll concentration obtained from MyOcean data centre and already remapped to the regular grid (L3C). Later, based on user’s request, we plan to extend number of datasets available for reconstruction. [less ▲]

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