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See detailBeta cell regeneration from pancreatic ducts in adult zebrafish
Manfroid, Isabelle ULg

Poster (2016, September 18)

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See detailObjective drowsiness monitoring to assess fitness for duty
François, Clémentine ULg; Hoyoux, Thomas ULg; Langohr, Thomas ULg et al

Poster (2016, September 16)

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See detailOn-farm Evolution of Genetic Diversity of Four Old Varieties of Phaseolus vulgaris L.
Caproni, Leonardo; Raggi, Lorenzo; Klaedtke, Stephanie ULg et al

Poster (2016, September 16)

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which ... [more ▼]

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which being made of different genotypes can be referred to as populations, evolved on-farm during three years of multiplication (from 2012 to 2014) in two different experimental sites (Brittany and Luxembourg) under organic farming conditions. The four populations (Flageolet Chevrier Vert, Rognon de Coq, Roi des Belges, Saint Esprit à Œil Rouge) were chosen among those grown by two small-scale organic seed farmers while the commercial variety (Calima) was included as control. In 2015 the initial and the evolved populations were grown in a common field and characterized with morpho-phenological traits. In the same year, young leaf tissues were collected from 94 samples per population (total of 470 samples) for DNA extraction and molecular characterization. Genotyping was carried out using 22 SSR loci and fluorescent PCR amplicons analysed on an ABI3130xl sequencer. Molecular data were then used for multivariate analysis, analysis of molecular variance and pair-wise Fst statistics calculation. Genotyping resulted in the production of about 20K data points. A total of 83 different alleles were identified in 16 polymorphic loci ranging from two (BMb293, BM156, BMd-44) to 18 (BMd-43). The first two axis of the Principal Coordinates analysis (PCoA) explained 76,13% of total diversity and clearly distinguished the five groups of populations. Analysis of Molecular Variance (AMOVA) showed that 76, 20 and 4% of total diversity is among populations, individuals and within individuals, respectively. Only for multiplications carried out in Luxemburg significant differentiation between the original populations and final populations were found in two old varieties (Flageolet Chevrier Vert and Rognon de Coq, Fst = 0.117 and 0.143, respectively, P≤0.001). The results showed that in Luxemburg farmer practices and pedo-climatic conditions significantly shaped the diversity of the two populations. This is confirmed by phenotypic observations, as these multiplications also significantly differed (p<0.05) for flowering date for all varieties except 'Roi des Belges'. [less ▲]

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See detailMetabolites from media supplemented with 3’-sialyllactose and fermented by bifidobacteria have an antivirulent effect against intestinal pathogens
Bondue, Pauline ULg

Poster (2016, September 16)

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides ... [more ▼]

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides (BMO) similar to HMO, which are mainly represented in colostrum by 3’-sialyllactose (3’SL) [2]. Bifidobacterium crudilactis, a species of bovine origin, encodes for β galactosidases and α-glucosidases and could therefore be able to metabolise those BMO [3; 4; 5]. In addition, fermentation products from bifidobacteria can produce antivirulent activity against intestinal pathogenic bacteria [6; 7]. This study focused on capacity of bifidobacteria to metabolise BMO, more particularly 3’SL, and on potential antivirulent effect of cell-free spent media (CFSM) against virulence gene expression of pathogenic bacteria. Material and methods B. bifidum BBA1 and B. crudilactis FR/62/B/3 isolated respectively from breastfed children feces and from cow raw milk cheese were grown on media supplemented with BMO or 3’SL, as sole source of carbon. The CFSM were harvested after centrifugation of cells culture, freeze-dried and concentrated 10 fold. Next, their effects were tested against virulence gene expression using ler and hilA promoter activity of luminescent constructs of Escherichia coli 0157:H7 ATCC 43888 and Salmonella Typhimurium SA 941256, respectively. The effect was confirmed on wild type strains of E. coli O157:H7 ATCC 43890 and S. Typhimurium ATCC 14028 using RT-qPCR. Results Both strains were able to grow in presence of whey or 3’SL, but B. crudilactis showed the best growth compared to B. bifidum. The highest cell concentrations were observed with media containing whey (8.9 ± 0.6 log cfu/ml and 8.1 ± 0.3 log cfu/ml, respectively). CFSM from fermented media supplemented with 3’SL resulted in under-expression of hilA and ler genes for the luminescent constructs and in under-expression of ler (ratios of -15.4 and -8.1) and qseA (ratios of -2.1 and -3.1) genes for the wild type strain of E. coli O157:H7. No effect was observed for the wild type strain of S. Typhimurium. Discussion B. crudilactis presented the best growth potential probably because its genome encodes the enzymatic machinery to use BMO (β galactosidases and α-glucosidases) [3; 4; 5]. The positive effect of media supplemented with milk products on growth of probiotics has been demonstrated previously [8]. CFSM obtained from media supplemented with 3’SL down-regulate several virulence genes of E. coli O157:H7 and potentially S. Typhimurium. This effect has been observed previously with CFSM obtained from fermentation of lactic acid bacteria or bifidobacteria, by production of antivirulent metabolites [2; 3]. BMO combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the exact nature of the active molecules. References 1. Garrido D. et al. (2013). Microbiology 159: 649-664. 2. Urashima T. et al. (2013). Biosci Biotechnol Biochem 77: 455-466. 3. Sela D. A. (2011). Int J Food Microbiol 149: 58-64. 4. Milani C. et al. (2014). Appl Environ Microbiol 80: 6290-6302. 5. Bondue P. & Delcenserie V. (2015). Korean J Food Sci Anim Resour 35: 1-9. 6. Medellin-Pena M. J. et al. (2007). Appl Environ Microbiol 73: 4259-4267. 7. Bayoumi M. A. & Griffiths M. W. (2012). Int J Food Microbiol 156: 255-263. 8. Champagne C. P. et al. (2014). Can J Microbiol 60: 287-295. [less ▲]

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See detailCHARACTERISATION OF THE STRUCTURAL, DYNAMIC AND AGGREGATION PROPERTIES OF THE W64R AMYLOIDOGENIC MUTANT OF LYSOZYME
Vettore, Nicola; Kumita, Janet; Moray, Joël ULg et al

Poster (2016, September 15)

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See detailDevelopment of a semiochemical-based control method against the walnut husk fly, Rhagoletis completa Cresson
Sarles, Landry ULg; Lognay, Georges ULg; Verhaeghe, Agnès et al

Poster (2016, September 15)

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See detailExtremophile plants as source of biopesticides against European damageable plant pathogens
Ben Kaab, Sofiène ULg; Parisi, Olivier ULg; De Clerck, Caroline ULg et al

Poster (2016, September 14)

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt ... [more ▼]

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt and drought) increase production and accumulation of reactive oxygen species (ROS). Consequently, extremophile plants have developed adaptive responses including the synthesis of specific bioactive molecules used for medical and nutritional purposes (Ksouri et al., 2012). In that context, the main objective of the present study was the identification of effective plant extracts and essential oils from extremophile plants against the most important plant pathogens in Europe (in term of loss, treatment necessity and/or cost). The study began with the selection of four endemic medicinal species suspected to be antimicrobial due to their wealth of phenolic and terpene compounds, such as flavonoids, phenolic acids, and coumarins (Ksouri et al., 2012). Each of the aerial plant materials was grounded and macerated with solvent (methanol or chloroform) for 24 h. The solvent was then eliminated along rotavapor. The yield of plant extract varied between 1.56 and 6.7%. Kinetics of growth of the 3 pathogens cultivable in liquid medium was determined before testing the impact of plant extracts and essential oils. Methanolic and chloroform plant extracts (EM1, EM2, EC1 and EC2) and essential oils (EO1, EO2) were compared for their antifungal potential. The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts was analyzed by GC/MS. Yield of essential oil varied between 0.7 and 1.2%. Therefore, antifungal activity of plant extracts and essential oils was evaluated using ELISA microplates with a blocked randomized design, as described previously (Parisi et al., 2013). The results obtained showed that EM2 at 7 mg/ml has a very high fungistatic activity against Fusarium culmorum, F. oxysporum and Penicillium italicum. It was characterized with a high amount of polyphenols, flavonoids and condensed tannins. Statistical analysis showed that the efficiency of methanol extracts significantly differed from those of the chloroform extracts. In addition, essential oils significantly reduced spores germination in a dose-dependent manner. Their fungistatic activity reached 100% at 6000 ppm. In conclusion, this work allowed us to open new perspectives on the application of extremophile plant extracts as novel biocontrol strategy against plant pathogens. [less ▲]

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See detailFirst come first served: “priority effect“ benefits Ambrosia artemisiifolia L. more than other ruderal Asteraceae species
Ortmans, William ULg; Mahy, Grégory ULg; Monty, Arnaud ULg

Poster (2016, September 14)

Ambrosia artemisiifolia L. (common ragweed, Asteraceae) is an invasive weed causing a health crisis in Europe, due to its highly allergenic pollen. In Western Europe the invaded range covers most of ... [more ▼]

Ambrosia artemisiifolia L. (common ragweed, Asteraceae) is an invasive weed causing a health crisis in Europe, due to its highly allergenic pollen. In Western Europe the invaded range covers most of central and southern France, and northern Italy. Northwards beyond the edge of this range, occurrence of casual population have been described for years, but these populations do not appear to become invasive, and the species does not seem to spread. This situation raises the following question: Has the invaded range reached a limit or will the species continue its invasion northwards? To answer this question, we followed two complementary approaches. First we set up an experimental garden in Belgium, 250 km north to the current invaded range, to see if the local climate allows the completion of the species reproduction cycle. Second, we performed an in situ measurement campaign in 12 population located beyond the edge, within the range but near the margin, and in the center of the invaded range. The aim of this campaign was to test whether the species had reduced plant performance towards range margins. The results showed that the species is able to establish populations with high growth rates in Belgium. Furthermore, the species expressed similar performance across the considered areas, even beyond the current invasion front. No evidence of processes constraining the invasion was found, which suggests a great potential for invasion north to the current invaded range. In this uncertain situation, awareness actions should be considered in the northern countries. [less ▲]

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See detailEffects of seed traits variation on seedling performance of the invasive weed, Ambrosia artemisiifolia
Monty, Arnaud ULg; Mahy, Grégory ULg; Ortmans, William ULg

Poster (2016, September 14)

Seedling performance can determine the survival of a juvenile plant and impact adult plant performance. Understanding the factors that may impact seedling performance is thus critical, especially for ... [more ▼]

Seedling performance can determine the survival of a juvenile plant and impact adult plant performance. Understanding the factors that may impact seedling performance is thus critical, especially for annuals, opportunists or invasive plant species. Seedling performance can vary among mothers or populations in response to environmental conditions or under the influence of seed traits. However, very few studies have investigated seed traits variations and their consequences on seedling performance. Specifically, the following questions have been addressed by this work: 1) How the seed traits of the invasive Ambrosia artemisiifolia L. vary among mothers and populations, as well as along the latitude; 2) How do seed traits influence seedling performance; 3) Is the influence on seedlings temperature dependent. With seeds from nine Western Europe ruderal populations, seed traits that can influence seedling development were measured. The seeds were sown into growth chambers with warmer or colder temperature treatments. During seedling growth, performance-related traits were measured. A high variability in seed traits was highlighted. Variation was determined by the mother identity and population, but not latitude. Together, the temperature, population and the identity of the mother had an effect on seedling performance. Seed traits had a relative impact on seedling performance, but this did not appear to be temperature dependent. Seedling performance exhibited a strong plastic response to the temperature, was shaped by the identity of the mother and the population, and was influenced by a number of seed traits. [less ▲]

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See detailPAI Annual Meeting
Ouhib, Farid ULg; Aqil, Abdelhafid ULg; Dirani, Ali et al

Poster (2016, September 12)

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See detailInvolvement of membrane remodelling induced by fatty acids in the regulation of the NLRP3 inflammasome activity in human macrophages
Gianfrancesco, Marco ULg; Dehairs, Jonas; Bloch, Katerzina et al

Poster (2016, September 12)

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See detailOrganocobalt complexes as source of radicals for the controlled polymerization of unconjugated monomers
Demarteau, Jérémy ULg; Cordella, Daniela ULg; Kermagoret, Anthony et al

Poster (2016, September 12)

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See detailSynthesis and characterizations of non-isocyanate polyurethane (NIPU) hydrogels
Gennen, Sandro ULg; Grignard, Bruno ULg; Thomassin, Jean-Michel ULg et al

Poster (2016, September 12)

Polyurethane (PU) is on of the most used polymers for the preparation of hydrogels due to its good biocompatibility, biodegradation and excellent mechanical properties. PU hydrogels are found in lot of ... [more ▼]

Polyurethane (PU) is on of the most used polymers for the preparation of hydrogels due to its good biocompatibility, biodegradation and excellent mechanical properties. PU hydrogels are found in lot of applications such as wound dressing, soft contact lenses, drug delivery and scaffolds for tissue engineering. Classicaly, PU is produced by a step-growth polymerization between diols and diisocyanates. However, in order to avoid the use of harmful isocyanates compounds and because of regulations which tend to ban the use of isocyanates, we developed hydrogels based on a non-isocyanate polyurethane (NIPU) chemistry by valorizing CO2-sourced cyclic carbonates and amines. Precisely, NIPU hydrogels were prepared by a solvent-free copolymerization between bifunctional hydrophilic polyethylene glycol cyclic carbonates and diamines in presence of a triamine as a crosslinker, followed by a water swelling of the obtained cross-linked gel. Parameters such as the cross-linking ratio and diamine’s nature were optimized. Different clay contents (cloiste 30B) as nanofiller were dispersed in the ideal cyclic carbonate/diamine/triamine formulation prior polymerization in order to reinforce the compression properties of NIPU hydrogels. Finaly, we were able to prepare NIPU hydrogels with water content up to 80 % and good compression properties using low clay content. [less ▲]

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See detailPolyphosphoester containing amphiphilic block copolymers as drug nanocarriers
Ergül, Zeynep ULg; Vanslambrouck, Stéphanie; Thiry, Justine ULg et al

Poster (2016, September 12)

The design of drug delivery systems often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, supramolecularly self-assembled ... [more ▼]

The design of drug delivery systems often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, supramolecularly self-assembled amphiphilic block copolymers into spherical micelles are appropriate carriers for poorly soluble drugs. In that framework, we have designed novel functional poly(ethylene oxide)-b-polyphosphoester amphiphilic block copolymers able to cross-linked under UV and degrade in response to a reduction of the pH from neutral conditions. Therefore, an unsaturated alkene side-chain was introduced on the cyclic phosphate monomer according to a one-step reaction followed by its organocatalyzed polymerization initiated by a poly(ethylene oxide) macroinitiator. After self-assembly into water, the micelles were cross-linked by UV irradiation. Then, these cross-linked micelles have been loaded by doxorubicin, i.e. a drug used in cancer therapy. We observed that the doxorubicin loading increased with the number of double bonds on the polyphosphate block of non-cross-linked micelles. This diblock amphiphilic copolymer bearing pendant unsaturations appears thus particularly promising candidate to build micellar drug delivery systems for intravenous injection. [less ▲]

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See detailc-Jun regulates altered pre-mRNA splicing in response to cisplatin
Deward, Adeline; Gabriel, Maude; Klinck, Roscoe et al

Poster (2016, September 11)

Genotoxic stress is a well-known inducer of pre-mRNa alternative splicing . In this work, we aim at identifying keys componants of the signaling cascade linking the DNA lesion to the splcing machiney.and ... [more ▼]

Genotoxic stress is a well-known inducer of pre-mRNa alternative splicing . In this work, we aim at identifying keys componants of the signaling cascade linking the DNA lesion to the splcing machiney.and thus gain better knowledge od the molecular mechanism controling large scale splcing decision in stress situation. Nous avons établi que C-Jun est important pour l'épissage alternatif de nombreux ARN pré-messagers. [less ▲]

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See detailSelf-defining memories and future projections as a means to facilitate reflection on life values in depressed adults: A preliminary study
Wagener, Aurélie ULg; Brouette, Béatrice ULg; Blairy, Sylvie ULg

Poster (2016, September 10)

Introduction. In the Brief Behavioral Activation Treatment for Depression, depressed patients are lead to a reflection on their personal values per life domains (e.g., family relationships, employment ... [more ▼]

Introduction. In the Brief Behavioral Activation Treatment for Depression, depressed patients are lead to a reflection on their personal values per life domains (e.g., family relationships, employment). The aim of this reflection is to tailor behavioral assignments through valued activities. This reflection is usually difficult for depressed patients. The current study hypothesized that exercises on autobiographical memories might act as a means to facilitate the reflection on personal values. In this perspective, self-defining memories (SDMs) and self-defining future projections (SDFPs) appear to be particularly relevant. SDMs and SDFPs concern reflections on the past and on the potential future central goals, values and conflicts of one’s life. SDMs and SDFPs are vivid and well-rehearsed autobiographical memories related to a meaning-making (i.e., a learned lesson about oneself, others or the world). The emotional valence of SDMs and SDFPs can either be positive, neutral or negative. Further, SDMs and SDFPs can consist in either specific events or categorical ones. Due to the relevance of these memories for the self, it is to be expected that recalling SDMs and imagine SDFPs will help individuals to have a clearer view of their personal values which in turn might facilitate the selection of valued activities. Further, it is hypothesized that these exercises might decrease depression and disturbances of psychological processes (i.e., rumination, self-concept clarity, environmental satisfaction). Therefore, this study aims were two-fold. First, it aimed at assessing the feasibility for depressed patients to recall SDMs and to imagine SDFPs per life domains. Second, it aimed at assessing the impact of these exercises on depression and psychological processes. Method. Nineteen depressed patients and 17 paired healthy controls (25-60 years) were recruited (data collection is still ongoing). This study comprises four meetings. During the first and the last meetings, participants completed self-reported assessments of depression and psychological processes. During the third and fourth meetings, participants were asked either to recall SDMs or to imagine SDFPs per life domains. SDMs and SDFPs were rated on specificity, meaning-making and emotional. The impact of these exercises was assessed on depression and psychological processes. Results. Preliminary results indicate that depressed patients are as able as healthy controls to recall SDMs and imagine SDFPs per life domains. Depressed patients recall less SDMs and SDFPs with meaning-making than healthy controls. Depressed patients report less SDMs and less SDFPs related to employment and less SDMs related to hobbies than healthy controls. Preliminary results show a decrease of depression in depressed patients only. Discussion. Even though depressed patients are as able as the healthy controls to recall SDMs and to imagine SDFPs, preliminary results highlight significant differences between depressed patients and healthy controls on SDMs and SDFPs. Preliminary results indicate that SDMs and SDFPs positively impact depression in depressed patients. Experimental and clinical implications of these findings will be discussed during the conference. [less ▲]

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See detailInvolvement of Ikbζ in glioblastomas and its potential implication in radioresistance
Dubois, Nadège; Willems, Marie; Kroonen, Jérôme et al

Poster (2016, September 09)

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See detailWhy a culture collection of Cyanobacteria?
Wilmotte, Annick ULg; Renard, Marine ULg; Simons, Véronique

Poster (2016, September 08)

The BCCM/ULC public collection is funded by the Belgian Science Policy Office since 2011 and an ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi ... [more ▼]

The BCCM/ULC public collection is funded by the Belgian Science Policy Office since 2011 and an ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. The collection aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths…) and make it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, and genomic make-up. It presently includes 226 cyanobacterial strains, with 120 being of (Sub) Antarctic origin (http://bccm.belspo.be/catalogues/ulc-catalogue-search). The morphological identification shows that the strains belong to the orders of Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. [less ▲]

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See detailCyanobacterial Diversity In Antarctic Aquatic Microbial Mats
Stelmach Pessi, Igor ULg; Lara, Yannick ULg; Durieu, Benoit ULg et al

Poster (2016, September 08)

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See detailMagnetic shielding above 1 T at 20 K with bulk, large grain YBCO tubes made by Buffer-aided Top Seeded Melt Growth.
Wera, Laurent ULg; Fagnard, Jean-François ULg; Kumar, Nambury Devendra et al

Poster (2016, September 07)

A superconducting tube can be used as an efficient magnetic shield. The best shielding performance levels are usually achieved when the tube is closed at one or both extremities. The purpose of the ... [more ▼]

A superconducting tube can be used as an efficient magnetic shield. The best shielding performance levels are usually achieved when the tube is closed at one or both extremities. The purpose of the present work is to study experimentally the shielding performance of YBCO tubes obtained by Buffer-aided Top Seeded Melt Growth fabrication process (BA-TSMG). This fabrication process enables the tube to be closed at one extremity by a cap containing the seed and there is no air gap between the cap and the tube. The shielding effectiveness is characterized by two parameters: (i) the shielding factor, defined as the ratio between the applied magnetic induction and the local magnetic induction measured inside the shield, and (ii) the threshold induction, i.e. the applied magnetic induction above which a given value of the shielding factor cannot be achieved. The magnetic shielding performances of tubes with different geometry are characterized at 77 K in liquid nitrogen. Further magnetic shielding measurements are carried out on one tube at various temperatures ranging from 20 K to 77 K using a Physical Property Measurement System (PPMS). The tubes are subjected to a quasi-static (“DC”) uniform magnetic field. A Hall probe measures the axial component of the local magnetic induction along the axis of the tube as a function of the applied magnetic induction. In order to investigate how the cap prevents the magnetic flux from penetrating inside the tube, we also characterize open tubes where the cap is removed and compare their properties to those of closed tubes. Magnetic shielding measurements show that the threshold induction increases by a factor of 9 as temperature decreases from 77 K to 20 K. Measurements also show that the presence of the cap improves the shielding performance at the closed extremity of the order of 1000 as it reduces the penetration through the open end. Near the closed extremity, a threshold induction of 1.5 T was reached at 20 K. To our knowledge this threshold induction is the best value reported so far at 20 K, and is comparable in magnitude to the record threshold inductions reported for bulk MgB2 and Bi-2212 materials at lower temperatures. These results give evidence that efficient magnetic shields can be obtained with this fabrication technique. [less ▲]

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See detailHow do droplets form?
Van Loo, Stéphanie ULg; Stoukatch, Serguei ULg; Kraft, Michael ULg et al

Poster (2016, September 07)

In microfluidics, flow focusing is widely used to produce water-in-oil droplets in microchannels at high frequency. We here report an experimental study of droplet formation in a microfluidic cross ... [more ▼]

In microfluidics, flow focusing is widely used to produce water-in-oil droplets in microchannels at high frequency. We here report an experimental study of droplet formation in a microfluidic cross-junction with a minimum number of geometrical parameters. We mostly focus on the squeezing regime, which is com- posed of two distinct steps : filling and pinching. The duration of each step (and corresponding volumes of each liquid phase) are analyzed. They vary according to both water and oil flow rates. These variations provide several insights about the fluid flows in both phases. We propose several scaling laws to relate the droplet volume and frequency to the flow rate of both phases. We also discuss the influence of surfactant and channel compliance on droplet formation. [less ▲]

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See detailAre Christmas tree plantations a suitable habitat for farmland birds?
Gailly, Robin ULg; Paquet, Jean-Yves; Titeux, Nicolas et al

Poster (2016, September 06)

Over the last decade, nonfood perennial crops have been increasingly prevalent in European farming systems. Here, vegetation structure and crop management greatly differ from conventional crops and may ... [more ▼]

Over the last decade, nonfood perennial crops have been increasingly prevalent in European farming systems. Here, vegetation structure and crop management greatly differ from conventional crops and may likely impact farmland birds. Impacts on birds have been examined for bioenergy agricultural systems, such as miscanthus plantations and short rotation willow coppice. In Europe, the extend of Christmas tree plantations (CTP) has recently increased dramatically in farmland but their impacts on farmland birds remains largely unknown. We examined the extent to which CTP in southern Belgium alter bird species assemblages compared to traditional farmland. The presence and abundance of birds were recorded twice during a breeding season in randomly selected sites located in farmland areas with and without CTP. Results show that the conversion of traditional farmland into CTP modifies bird species assemblages and locally increases bird diversity and density without inducing biotic homogenization. This observed pattern indicates that CTP might constitute an interesting alternative habitat for farmland birds. However bird presence or abundance may be confounding indicator of habitat quality because individuals may be attracted to low-quality habitats in human modified landscapes. The European Stonechat Saxicola rubicola was chosen as a model species to investigate the quality of CTP as a breeding habitat in more details. Habitat quality in CTP was assessed with measures of reproductive success and survival. These measures were compared with those collected in traditional farmland. Although vegetation structure, field management and nest placement are highly different between habitat types, our first results suggest that CTP are not of lesser quality than traditional farmland for the European Stonechat. [less ▲]

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See detailContribution of Cross-linking and Ion-mobility for the study of protein and complex structures
Baumans, France ULg; Grifnée, Elodie ULg; Hage, Christoph et al

Poster (2016, September 05)

The tridimensional structures of proteins and the mapping of protein-protein interactions are precious sources of information for the understanding of their function. Different techniques such as X-ray ... [more ▼]

The tridimensional structures of proteins and the mapping of protein-protein interactions are precious sources of information for the understanding of their function. Different techniques such as X-ray cristallography or nuclear magnetic resonance are usually used to achieve this goal. In the field of mass spectrometry, several tools were also developped. The one presented here is the chemical cross-linking in which two reactive residue side chains, spatially close, are linked thanks to a bifunctional chemical, called crosslinker. Ion-mobility coupled to mass spectrometry has also been investigated for the study of cross-linked products. The first results tend to show that cross-linkers allow to fix the shape of the protein in solution, leaving it intact when analysed in the gas phase. [less ▲]

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See detailFeeding ecology of Southern Ocean seastars inferred from stable isotopes ratios
Le Bourg, Baptiste ULg; Blanchard, Alice; Danis, Bruno et al

Poster (2016, September 05)

The Southern Ocean is currently subjected to strong and contrasted impacts of climate change. The Western Antarctic Peninsula is one of the most rapidly warming regions of the world, resulting in sea ice ... [more ▼]

The Southern Ocean is currently subjected to strong and contrasted impacts of climate change. The Western Antarctic Peninsula is one of the most rapidly warming regions of the world, resulting in sea ice cover decreases. Increasing seawater temperature and sea ice cover reduction in Western Antarctic Peninsula and associated regions will likely impact food web functioning through temperature-related changes in consumer physiology, modifications of benthic community structure (e.g. expansion of exogenous species such as predatory crabs), modifications of benthic-pelagic coupling intensity or disruption of benthic production. Asteroids (Echinoderms) are an important group of southern benthos. This group also has a great trophic variability and is potentially more resistant than other organisms to temperature changes (Peck et al. 2008). Consequently, they will be likely impacted by modifications in food webs functioning rather by direct warming and investigating their trophic ecology is necessary to infer how climate change will impact them. In this context, the aim of this study is to use stable isotopes ratios of C, N and S to infer sea stars trophic ecology. 16 species of sea stars spanning 10 different families sampled in multiple and contrasted habitats across Subantarctic (South Georgia, South Sandwich Islands, Falkland Islands) and Antarctic (South Shetland Islands, South Orkney Islands, Western Antarctic Peninsula) locations. In total, tegument samples from 213 specimens was analysed. Diversity and plasticity of asteroid diet along Southern Ocean coasts were explored through isotopic niche parametrisation (e.g. niche width and overlap between species and/or populations; Jackson et al. 2011). The data will also be used in a larger scale research project on the trophic ecology of Antarctic sea stars. This project will notably compare trophic resources supporting asteroid communities in Western Antarctic Peninsula, where sea ice cover is decreasing, and in Terre Adélie, where sea ice cover is increasing (Parkinson & Cavalieri 2012). Ultimately, this project will help understanding which ecological processes determine how an animal group copes with environmental modifications linked to climate change. [less ▲]

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See detailUnusually high sea ice cover influences resource use by benthic invertebrates in coastal Antarctica
Michel, Loïc ULg; Dubois, Philippe; Eleaume, Marc et al

Poster (2016, September 05)

Antarctica currently undergoes strong and contrasted impacts linked with climate change. While the West Antarctic Peninsula is one of the most rapidly warming regions in the world, resulting in sea ice ... [more ▼]

Antarctica currently undergoes strong and contrasted impacts linked with climate change. While the West Antarctic Peninsula is one of the most rapidly warming regions in the world, resulting in sea ice cover decrease, the sea ice cover of East Antarctica unexpectedly tends to increase, possibly in relation with changes in atmospheric circulation. Changes in sea ice cover are likely to influence benthic food web structure through modifications of benthic-pelagic coupling, disruption of benthic production and/or modifications of benthic community structure (i.e. resource availability for benthic consumers). Here, we studied shallow (0-20 m) benthic food web structure on the coasts of Petrels Island (Adélie Land, East Antarctica) during an event of unusually high spatial and temporal (two successive austral summers without seasonal break-up) sea ice cover. Using stable isotope ratios of C and N and the SIAR mixing model, we examined importance of 4 organic matter sources (benthic macroalgae, benthic biofilm, sympagic algae, suspended particulate organic matter) for nutrition of dominant primary consumers and omnivores. 14 invertebrate taxa including sessile and mobile polychaetes, gastropods, bivalves, sea stars, sea urchins and sea cucumbers were studied. Our results indicate that most benthic invertebrates predominantly relied on sympagic algae. Despite its very high abundance, trophic role of benthic biofilm seemed limited. However, interpretation of data was complicated by the peculiar ecophysiological features of Antarctic invertebrates, whose very low metabolic rates could be associated to low isotopic turnover and long time to reach isotopic equilibrium with their food items. Resource use by consumers from Adélie Land markedly differed from literature data about invertebrate diet in coastal Antarctica, suggesting 1) important influence of increased sea ice cover on benthic food web structure and 2) high spatial and/or temporal variation in the feeding habits of studied organisms, likely linked with a high degree of trophic plasticity. Our results provide insights about how Antarctic benthic consumers, which have evolved in an extremely stable environment, might adapt their feeding habits in response to sudden man-driven changes in environmental conditions and trophic resource availability. [less ▲]

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See detailIntegrated Project with Focus on Energy Transition and Circular Economy for Developing Engineering Students' Soft Skills
Léonard, Grégoire ULg; Pfennig, Andreas ULg; Toye, Dominique ULg et al

Poster (2016, September 05)

The present work reports the experience of an integrated project developed at the University of Liege for master students in chemical engineering. The goals are to promote the acquisition of soft skills ... [more ▼]

The present work reports the experience of an integrated project developed at the University of Liege for master students in chemical engineering. The goals are to promote the acquisition of soft skills and to consolidate technical knowledge by integrating and linking chemical engineering disciplines usually taught separately. A case study was selected to address some of the challenges related to energy transition: students had to design the energy system of a remote island and make it as energy independent and CO2-neutral as possible by 2030. The course of action during the academic year, the assessment of soft skills, and the tools offered to ease the mentoring and encourage the acquisition of soft skills are described. Not all implemented techniques performed equally well, and this project finally appeared to be a challenge for the teaching team as well. 1 Introduction and background Over the last few years, University authorities, industrial partners as well as national and international experts that evaluated the education quality of our Department (AEQES, CTI) strongly suggested that opportunities should be offered to students to increase their soft skills as part of their curriculum. Moreover, many developments in chemical engineering are related to energy transition and circular economy, which are both transdisciplinary to conventional lectures. In this paper, we present methods and mentoring tools developed to teach students technical and soft skills for multi-disciplinary topics. 2 Description of the integrated project Objectives and constraints were defined at the onset of the project for both technical and soft skills. The technical objective was to propose an energy system that would make Reunion Island as energy independent and CO2-neutral as possible by 2030. This idea originated in the challenge set by the Eurecha 2015 student contest[1], for which students had to design facilities for a sheikhdom: electricity, water recycling, production of fertilizers… In our case, Reunion Island (~850 000 inhabitants) was considered as a case study as it is remote, has large biomass resources and high potential for renewable energies. Besides the objectives mentioned above and in order to force students to look at chemical engineering processes, the treatment of wastewater was imposed as well as the use of a synthetic liquid fuel as energy carrier. The targeted soft skills included working in large groups of minimum 4 students, efficient communication of results in English - both written and oral -, ability to integrate knowledge from various disciplines, development of critical mind and demonstration of independent and creative thinking. 3 Course of actions A team of 8 professors and senior scientists mentored the project and contributed to its assessment. The 10-ECTS project was divided in two parts. In the fall semester, students made global energy balances to design the energy system that would fulfill the objectives. As a result, a Sankey diagram of the energy flows on Reunion Island by 2030 was produced to allow for an overview of the available Island’s resources and needs, as well as of processes that can make the link between resources and needs. In the spring semester, two processes identified in the first part, namely the synthesis of bio-ethanol and bio-methanol, were modelled in more details using commercial software. Different tools were used to encourage student initiatives and work: • The use of a shared on-line portfolio for students to gather their documents improved their internal communication, but this remained a marginal channel for communication with teachers • In the fall semester, students orally presented progress reports every two weeks. After a feedback to students, the teaching team met to discuss the achievements and set the objectives for the next two weeks. This was very positive for the communication inside the teaching team. However, presentations every fortnight implied a work overload for students that had to constantly focus on preparing the presentations. • From the beginning, students were strongly encouraged to reach out to field experts whose contacts were provided. However, they preferred to rely mostly on Internet as their main source of information and reached out only rarely for help and usually very late. • In the fall semester, students had to designate new team leaders in turn every fortnight. This was abandoned as it prevented the establishment of clear structures in the group, reducing its efficiency. • In the spring semester, work tables allowed students to work directly with the teacher specialized in their task. This was appreciated by students and teachers, and it needs to be further encouraged. • Help in the group organization and interactions was provided by the PSGO (psychology of groups and organizations). This also included videoscopy, i.e. filming the students during their presentations and analyzing the records with them. This help was appreciated by students. The assessment was based on technical results for 60%, and soft skills for 40%. The evaluation of technical skills was done partly by all teachers equally and partly by teachers whose expertise was the closest to the technical sub-tasks. For soft skills, efficient communication, creativity in the work and results and links with conventional lectures were assessed. Critical thinking was evaluated through the relevance of qualitative and quantitative results and discussions. Group work was assessed by the teachers as well as by students through mutual evaluation. 4 Conclusions and perspectives The integrated project gave students a first opportunity to improve their soft skills along with their technical knowledge. It also improved their communication skills and their fluency in English. The teaching team proposed different mentoring techniques to encourage efficient work, with varying results. Finally, as the assessment ignored soft skills improvements, it may be modified by evaluating soft skills all year long so both the final result and the observed improvements contribute to the grade. Reference Eurecha, The European Committee for the Use of Computers in Chemical Engineering Education, 2015. Announcement for student contest problem competition 2015. http://bari.upc.es/eurecha/. [less ▲]

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See detailBack on Track... in 3D
Marchal, Antoine ULg; Lejeune, Philippe ULg; De Bruyn, Nico

Poster (2016, September 04)

Ecological monitoring provides basic information on population status and distribution that is crucial for conservation, research and management strategies. Studies using tracks are controversial due to ... [more ▼]

Ecological monitoring provides basic information on population status and distribution that is crucial for conservation, research and management strategies. Studies using tracks are controversial due to past misuses tainted with subjectivity. Advances in photogrammetry literally add another dimension to the recording techniques, and geometric morphometrics provides an appropriate approach for the study of track shape variation. Tracks are back on track in conservation! [less ▲]

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See detailVisuo-spatial STM impairment link to a serial order component deficit in children with Velocardiofacial syndrome
Attout, Lucie ULg; Vossius, Line ULg; Noël, Marie-Pascale et al

Poster (2016, September 01)

Some previous studies showed that verbal short-term memory (STM) is better preserved than visuospatial STM in the velocardiofacial syndrome (VCFS), a neurodevelopmental disorder resulting from a 22q11.2 ... [more ▼]

Some previous studies showed that verbal short-term memory (STM) is better preserved than visuospatial STM in the velocardiofacial syndrome (VCFS), a neurodevelopmental disorder resulting from a 22q11.2 deletion. This deficit is generally assigned to a more global visuospatial skills deficit. However, for verbal STM, previous study suggested a specific deficit in the serial order component, processing considered as independent of modalities. In this study we proposed to explore the serial order component in visuospatial STM by contrasting simultaneous vs. sequential presentation of stimuli to maintain in children with VCFS, in order to see in what extent the deficit is global and resulting from visuospatial deficit or whether this deficit is specific to the serial order component. We tested a group of 27 children and adolescents with VCFS to a control group, matched on verbal IQ performance estimated. The VCFS group showed impaired performance on the sequential configuration but similar performance on the simultaneous condition. These results support the idea of an amodal serial order component in STM. The implication of serial order STM deficits on numerical cognition will be discussed. [less ▲]

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See detailTEACHING VETERINARY RADIOLOGY: DOES COMPARISON HELP?
Etienne, Anne-Laure ULg; Delguste, Catherine ULg; Busoni, Valeria ULg

Poster (2016, September 01)

Introduction/Purpose Comparison learning is an approach for learning complex visual tasks. As described in human medicine, by comparing radiographs with pathology and without pathology, veterinary ... [more ▼]

Introduction/Purpose Comparison learning is an approach for learning complex visual tasks. As described in human medicine, by comparing radiographs with pathology and without pathology, veterinary students could learn to discriminate relevant disease-related information to recognize the disease. We hypothesized that exposure to a training by side-by-side comparison of normal to abnormal radiographs would lead to higher visual diagnostic accuracy and possibly to a higher ability to describe the features of a known disease on canine thoracic radiographs. Material and methods Twenty veterinary students were randomly assigned to either a group that compared radiographs showing thoracic disease with normal images (group 1) or to a group that only studied abnormal radiographs (group 2). All students had their theoretical radiology teaching and 13 of them had also received the practical teaching of the study curriculum, evenly distributed between the 2 groups. Twenty-nine radiographs of small animal thorax were used. The procedure consisted in three experimenter-supervised phases: 1. training, 2. visual recognition test, 3. feature description test. In the training phase, each screen showed two radiographs with the name of the disease present in each. In the group 1 (pathology/normal condition), a radiograph of a patient and a normal image were shown next to each other. In the group 2 (pathology/pathology condition), two radiographs of patients with the same disease were shown next to each other. The 9 screens were presented twice, with the diseases in a different order for the first and second runs. A Mann-Whitney test was used to compare the success rates of groups of students. Results On visual recognition test, there was no statistical difference in visual diagnostic accuracy between groups. When students gave the wrong answer, they often diagnosed the item as another disease of similar distribution (diffuse or focal). Students who received the practical teaching and students of group 1 had a higher accuracy for normal thoracic radiographs. On feature description test, no significant effect of comparison learning was found, but focal diseases were better described than diffuse diseases with a significant difference between these. Discussion/Conclusions Results show that comparison with normal images did not help in recognizing or describing thoracic pathologies but helps to recognize normal images. [less ▲]

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See detailComputed tomography-guided injection of muscle-derived mesenchimal stem cells in the intervertebral disc of dogs affected by natural disc degeneration: clinical safety and intervertebral disc imaging assessment
Liotta, Annalisa Pia ULg; Sandersen, Charlotte ULg; Ceusters, Justine ULg et al

Poster (2016, September 01)

Introduction/Purpose: Pre-clinical randomized controlled animal trials have been conducted to evaluate the effects of mesenchymal stem cell (MSCs) transplantation on intervertebral disc (IVD) degeneration ... [more ▼]

Introduction/Purpose: Pre-clinical randomized controlled animal trials have been conducted to evaluate the effects of mesenchymal stem cell (MSCs) transplantation on intervertebral disc (IVD) degeneration. According to their histological results and to imaging assessment of the IVD, intradiscal injection of MSCs is effective, arresting or slowing IVDD process, and is associated with a low complication rate. Few of these studies have been conducted on canine artificially degenerated IVD, using bone marrow or adipose-derived MSCs. Therefore a systematic study on naturally degenerated IVD using MSCs obtained from autologous muscular tissue in dogs is still lacking. The aims of this study were to evaluate the clinical effects of intradiscal injection of muscle-derived MSCs and its effects on imaging features of the intervertebral disc. Methods: Eight experimental dogs were randomly included with the approval of the University’s Animal Care and Use Committee. The final inclusion criteria were the presence of naturally degenerated lumbosacral IVD detected on low-field magnetic resonance (MRI) images and the obtaining of 3 x 106 autologous muscle-derived MSCs. A computed tomography (CT) and MRI examination was performed before and 2 months after the procedure and 13 imaging parameters were assessed. Mesenchymal stem cells diluted in 0,2 ml of FRS Hypothermosol were injected in the lumbosacral IVD under CT-guidance. Clinical examinations were performed regularly during 1 month after the procedure. Results: Six dogs met the inclusion criteria. The remaining 2 dogs did not undergo intradiscal injections, but were used as control group. No major or minor complications were reported during the procedure. No abnormalities were noticed during the clinical examinations. No statistically significant variations of IVD imaging features were noticed before and after the injections. Discussion/Conclusions: Intradiscal injection of muscle-derived MSCs is clinically safe and it is not associated with any progression of the IVD degeneration, detected by CT or low-field MRI imaging. Further studies are needed to assess its efficacy as treatment for the canine natural IVD degeneration. [less ▲]

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See detailComputed tomographic findings of a far lateral lumbar disc extrusion in a dog
Rizza, Maïlis ULg; Bouvy, Bernard ULg; Shimizu, Naomi ULg et al

Poster (2016, September 01)

Introduction In human medicine, far lateral lumbar disc extrusion (FLLDE) represents 7 to 12% of all disc herniations and MRI is the method of choice for diagnosis. MRI findings of a FLLDE has been ... [more ▼]

Introduction In human medicine, far lateral lumbar disc extrusion (FLLDE) represents 7 to 12% of all disc herniations and MRI is the method of choice for diagnosis. MRI findings of a FLLDE has been reported in one dog and the aim of this case report is to describe computed tomographic (CT) findings of a FLLDE in a dog. Methods A six-year-old neutered female Beagle with a good general health status was presented with a month history of left hind limb pain with shivering of this limb. Clinical examination revealed a left hind proprioceptive deficit but no pain was elicited at palpation. Results A CT scan pre- and post- contrast studies of the lumbosacral spine and a myelographic-CT were performed. A 1.3x0.6x0.3 cm homogeneous hyperattenuating (+/- 350 HU) ovoid structure was observed at the left lateral aspect of L6-L7 intervertebral disc space. The L6-L7 intervertebral disc nucleus pulposus was calcified but the annulus fibrosus had a normal non-calcified attenuation and seemed intact apart from a very thin hyperattenuating line next to the ovoid structure. This change was well circumscribed by an even hyperattenuating rim (1000HU) mimicking a thin dense cortex and was in close proximity with the annulus fibrosus and the left transverse process of L7. Perineural fat was not observed and contrast enhancement was visualized at the level of the left sixth lumbar nerve root with impingement and thickening of this root. No compression of the spinal cord was observed at this level on the myeloCT. These findings were suggestive of a dystrophic mineralization or an osteochondromatosis. FLLDE was considered less likely because of the almost normal appearance of the annulus fibrosus. At surgery some mixed gelified calcified material consistent with disc material was removed at the level of the left L6-L7 nerve root tract and disc fenestration was performed. The histological analysis confirmed the presence of degenerated herniated vertebral disk. Discussion/Conclusions In human medicine, MRI and CT scan are the main diagnostic modalities employed for diagnosis of FLLDE. In veterinary medicine, CT is often used to assess spinal diseases because of its availability. To the authors’ knowledge, CT findings of a FLLDE have not been described previously in dogs. Moreover, the thin dense cortex appearance surrounding the disc material was surprising. In conclusion, FLLDE should be included in the differential diagnosis of a calcified ovoid structure lateral to the spine even if the annulus fibrosus appears normal and if this structure is in close relation with the vertebral transverse process. [less ▲]

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See detailRefractive and diffractive contribution of linear chromatic aberration (LCA) on depth-of-focus with trifocal intraocular lenses (IOLs)
Loicq, Jerôme ULg; Gatinel, Damien

Poster (2016, September)

Purpose: To investigate the refractive and diffractive contribution of LCA on depth of focus extension of trifocal IOLs in polychromatic light conditions Setting: University of Liège, Belgium; Fondation ... [more ▼]

Purpose: To investigate the refractive and diffractive contribution of LCA on depth of focus extension of trifocal IOLs in polychromatic light conditions Setting: University of Liège, Belgium; Fondation Ophtalmologique A. de Rothschild, Paris Methods: The LCAs associated with the three focal points of hydrophobic and hydrophilic diffractive FineVision trifocal IOLs (PhysIOL SA, Liège, Belgium), were simulated in an Arizona eye model and experimentally measured on an optical bench at 480, 546 and 650 nm. The effect of Abbe number and aperture on different IOL materials was also evaluated. Based on wavelength–dependent MTF through-focus curves and PSF properties, polychromatic behavior of the trifocal IOLs was assessed under mesopic and photopic conditions. Results: LCA amplitude and sign were different for each of the trifocal IOL focal points. The diffractive LCA for near and intermediate was independent of IOL material (GFree versus hydrophilic acrylic, 26%), while far vision LCA appeared to be controlled by the material Abbe number. Under polychromatic conditions, the LCA contributed to depth of focus extension with different types of lens material, providing maximal visual acuity under white light conditions at all distances. Conclusions: Diffractive trifocal IOLs show chromatic aberrations with an increase in depth of focus under polychromatic light. This effect likely contributes to the extended range of vision. [less ▲]

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See detailSIMULATION AND ANALYSIS OF RECEPTOR DYNAMICS IN A BMP REGULATORY NETWORK
Germain, Morgan ULg; Bolander, Johanna; Ji, Wei et al

Poster (2016, September)

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See detailPlant esterified oxylipins: structure – function relationship
Genva, Manon ULg; Andersson, Mats X.; Nasir, Mehmet Nail ULg et al

Poster (2016, September)

In the present work, high quantities of arabidopsides were extracted and purified from Arabidopsis thaliana L.

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See detailComparaison de deux méthodes statistiques explorant la relation entre régimes alimentaires et risques cardiovasculaires.
Sauvageot, Nicolas ULg; Leite, Sonia; Donneau, Anne-Françoise ULg et al

Poster (2016, September)

Introduction L’analyse en composantes principales (ACP) et la régression à rangs réduits (RRR) sont deux méthodes de réduction de dimension permettant de construire des variables reflétant des régimes ... [more ▼]

Introduction L’analyse en composantes principales (ACP) et la régression à rangs réduits (RRR) sont deux méthodes de réduction de dimension permettant de construire des variables reflétant des régimes alimentaires. Les régimes alimentaires obtenus par ACP sont associés aux habitudes alimentaires de la population étudiée tandis que ceux obtenus par RRR sont associés à des indicateurs d’intérêt. L’objectif de cette étude est de comparer les deux approches quant aux régimes alimentaires obtenus et à leurs associations avec les facteurs de risques cardiovasculaires (FRCV). Méthodologie Les données proviennent de l’étude interrégionale et transversale nutrition, environnement et santé cardiovasculaire (NESCAV) ayant pour but de décrire la santé cardiovasculaire de la grande région (Luxembourg, Lorraine et Wallonie). Les apports alimentaires ont été mesurés à l’aide d’un questionnaire de fréquence alimentaire et 2298 individus ont été retenus pour cette analyse. Les indicateurs d’intérêt retenus pour l’approche RRR sont les FRCV usuels (obésité, hypertension, diabète, dyslipidémie). Résultats Nous avons trouvé que les régimes alimentaires consommés par la population et ceux associés au FRCV étaient similaires. Les régimes riches en fruits, fruits secs et oléagineux, légumes, huile d’olive, graisse riche en oméga 6, thé, et ceux pauvres en aliments frits, viandes, charcuteries, soda, plats préparés, bières étaient associés à une diminution des FRCV. Les autres régimes caractérisés par des apports élevés en aliments frits, viandes, abats, bières, vins, apéritifs et liqueurs et de faibles apports en céréales, sucres et soda étaient associés à une augmentation des FRCV. Conclusions L’utilisation des deux méthodes est utile pour obtenir des informations permettant d’élaborer de nouvelles recommandations alimentaires. Les régimes alimentaires identifiés dans cette étude sont à la fois associés aux habitudes alimentaires de la population et aux FRCV. [less ▲]

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See detailElastocapillarity in Dock Beetle Adhesion
Gernay, Sophie-Marie ULg; Federle, Walter; Lambert, Pierre et al

Poster (2016, September)

Miniaturization is currently limited by our ability to manipulate micrometer-sized objects. Insect adhesion is an advantageous bio-inspired solution to this problem, although it is not fully understood ... [more ▼]

Miniaturization is currently limited by our ability to manipulate micrometer-sized objects. Insect adhesion is an advantageous bio-inspired solution to this problem, although it is not fully understood yet. Interference Reflection Microscopy images of the beetle Gastrophysa Viridula adhesive structures in contact with a smooth substrate were analyzed. The results were cross-linked with an analytical model of an elastic beam deflected by a liquid meniscus. By fitting the unknown model parameters to the experimental data, we obtained information about the elastic properties of the structures, the liquid volume in the bridge, and the resulting adhesion levels. [less ▲]

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See detailExperimental Assessment of The Influence of Interface Geometries on Structural Response
Dossogne, Tilàn ULg; Jerome, Trevor; Lancereau, Damien et al

Poster (2016, September)

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See detailChemical profiling of major components in natural waxes to elucidate their roles in liquid oil structuring
Diem Doan, Chi; Ming To, Chak; Lynen, Frederik et al

Poster (2016, September)

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See detailSingle-Molecule Force Spectroscopy on Synthetic Helical Nanoarchitectures
Devaux, Floriane ULg; Li, Xuesong; Huc, Ivan et al

Poster (2016, September)

Inspired by the many folded conformations of the molecular machineries in nature, chemists have been developing the syntheses of artificial folded molecular architectures, namely foldamers. The ... [more ▼]

Inspired by the many folded conformations of the molecular machineries in nature, chemists have been developing the syntheses of artificial folded molecular architectures, namely foldamers. The investigation of these molecules using AFM-based Single Molecule Force Spectrosocopy (SMFS) allows the elucidation of both mechanochemical properties and conformational dynamics on the unimolecular scale in solution. The stepwise synthesis of aromatic oligoamide-based foldamers was designed to produce well-defined helically-folded molecular architectures. A PEO tether was coupled to one end of the foldamer. SMFS pulling experiments on this system yielded specific and reproducible force-extension patterns characteristic of single foldamers. Those patterns were further analyzed to determine unfolding forces and dynamics as well as to propose mechanistics hypotheses of the unfolding process. Several helical foldamers presenting variable lengths were considered. The force values measured for those foldamers are higher than those previously measured in natural biopolymers showing a high stability under a load and a propensity for the development of emergent properties. In addition, the increased stability of these aromatic oligoamide foldamers was confirmed by the observation of almost instantaneous reversibility of the unfolding under load. [less ▲]

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See detailAn innovative non-animal chitosan hydrogel is able to restore the rheology of osteoarthritis synovial fluid ex vivo
Henrotin, Yves ULg; Rocasalbas, Guillem; Chausson, Mickaël et al

Poster (2016, September)

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See detailRadioscopy laboratory diagnostic of epizootic diseases in Belgium and European countries
Cargnel, Mickaël ULg; Roelandt, Sophie; Van der Stede, Yves et al

Poster (2016, September)

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See detailInvestigation of the coagulation system in canine idiopathic pulmonary fibrosis
Roels, Elodie ULg; Bauer, N.; Lecut, Ch. et al

Poster (2016, September)

Detailed reference viewed: 19 (1 ULg)
See detailThe BCCM/ULC collection to conserve and study the biodiversity of Polar cyanobacteria
Wilmotte, Annick ULg; Renard, Marine ULg; Lara, Yannick ULg et al

Poster (2016, September)

The BCCM/ULC public collection of Cyanobacteria has been funded since 2011 by the Belgian Science Policy Office. BCCM/ULC is currently holding 226 cyanobacterial strains, with 119 being of Antarctic ... [more ▼]

The BCCM/ULC public collection of Cyanobacteria has been funded since 2011 by the Belgian Science Policy Office. BCCM/ULC is currently holding 226 cyanobacterial strains, with 119 being of Antarctic origin (including 3 from the sub-Antarctic). The cyanobacteria constitute the bacterial phylum with the largest morphological diversity and their taxonomy is still a work in progress. In Polar Regions, Cyanobacteria represent key primary producers and are important drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build extensive benthic microbial mats in lakes and soil crusts in terrestrial biotopes. They have adapted to their environment, and may present interesting features to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other stresses. In this poster, we present the results of the 16S rRNA phylogenetic analysis for 76 Antarctic strains. This allows us to illustrate the diversity present in the collection, to detect lineages for which no genome has yet been sequenced, and to pinpoint taxonomic problems that should be addressed in a more comprehensive study. [less ▲]

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See detailIsolation of bacteriophages against Klebsiella pneumoniae and in vivo activity
Thiry, Damien ULg; Passet, Virginie; Dufour, Nicolas et al

Poster (2016, September)

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains ... [more ▼]

Klebsiella pneumoniae is a bacterial pathogen able to induce severe healthcare-associated or community-acquired infections in humans and animals. The constant emergence of antibiotic resistant strains reinforces the need to find alternatives to antibiotic treatments. The use of bacteriophages is a promising approach. The aim of this study was to isolate bacteriophages directed against K. pneumoniae strains and to test their efficacy in a murine model. Bacteriophages against five different K. pneumoniae (2 of capsular type K1 and K2 and 1 undetermined) were isolated and purified from waste water collected in Paris area. The morphology of plaques (zones of bacterial killing) was recorded and several of them were purified three times by successive replating. Phage titers were determined by serial dilutions on their respective hosts as well as on 18 other Klebsiella strains to identify their host range. Kinetics of bacterial lysis were monitored during 15h at 3 multiplicities of infection, in triplicates. For in vivo experiment, a total of 10 mice were inoculated with 200 µl of K. pneumoniae (4.6E+07 CFU) by oral gavage and the level of K. pneumoniae in fecal samples was monitored for 10 days. Five mice did not receive any treatment and 5 other mice received a cocktail of three bacteriophages (8E+07 PFU) at day 4 post-inoculation. A total of 54 bacteriophages were isolated and purified with titers ranging from 2E+5 to 3.6E+10 PFU/ml. The host range study showed that bacteriophages against K. pneumoniae have a specificity related to the capsular type of their bacterial host. Lysis kinetics of bacteria suggested that different phages were isolated. Despite difficulties with the murine intestinal model, evidence was obtained that bacteriophages are able to reduce intestinal carriage. Our results show that bacteriophages isolated against K. pneumoniae are specific for a given capsular type, although further studies are necessary to provide more details on this capsular specificity and its molecular determinants. To fully address the in vivo potential of phages, a reliable mouse model of intestinal carriage of K. pneumoniae strains needs to be established. [less ▲]

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See detailTargeted mutagenesis of orphan GPCRs of the SREB family
Laschet, Céline ULg; Dupuis, Nadine ULg; Soni, Arvind ULg et al

Poster (2016, September)

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See detailIdentification of virulotypes and serotypes of enteropathogenic (EPEC) and Shigatoxigenic (STEC) Escherichia coli from healthy cattle at slaughterhouses in Wallonia.
Takaki, Shino; Duprez, Jean-Noël ULg; Fakih, Ibrahim et al

Poster (2016, September)

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the ... [more ▼]

Escherichia coli producing the attachment-effacement (AE) lesion (EPEC) and/or Shiga toxins (STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the intestines of healthy cattle. Besides the O157:H7 serotype, which is the main serotype causing STEC outbreaks in the world EPEC and STEC can belong to dozens of O serogroups. Of them, 9 have been frequently identified worldwide: O5, O26, O103, O104, O111, O118, O121, O145 and O165. The aim of this study is to identify the virulotypes and the O serotypes of EPEC and STEC isolated from healthy cattle at slaughterhouses in Wallonia. A total of 245 faeces (216 <1year-old bulls, 25 cows and 4 heifers) were sampled between April and June 2014 in 2 slaughterhouses in Wallonia and grown overnight at 37°C in Lauryl sulfate Enterobacteriaceae selective broth. The enrichment broths were assayed with an stx1, stx2 (Shiga toxin) and eae (AE lesion) triplex PCR and positive broths were inoculated onto 4 agar media: McConkey’s, Chromagar ES, Chromagar ES with tellurite and Chromagar STEC. Up to ten colonies per plate were picked up, sub-cultured and tested by the colony hybridization assay with gene probes targeting the stx1, stx2 and eae genes. The triplex PCR was again performed on all probe-positive isolates. The PCR-positive E. coli were subsequently assayed with two pentaplex PCR targeting the specific genes coding for the ten O serogroups listed above. Of the 2563 sub-cultured isolates, 744 isolates (29%) from 62 animals (25%) tested positive with the colony hybridization assay. Of them, 687 isolates (92%) from 59 animals were positive with the triplex PCR and the results of both tests were in agreement for 617 isolates (83%). One to 29 isolates per animal were probe- and PCR-positive. The positive isolates grew on Chromagar STEC (379; 55%), on Chromagar ES with tellurite (189; 28%), on Chromagar ES (62; 9%) or on McConkey’s agar (57; 8%). The most frequent virulotypes were eae+ (EPEC: 372 isolates; 54%), eae+stx1+ (AE_STEC: 119 isolates; 17%) and stx2+ (STEC: 118 isolates; 17%). In some animals different virulotypes were identified. The serogrouping with the two pentaplex PCR is in progress. AE-STEC, EPEC and STEC are excreted by 25% of the healthy cattle at slaughterhouses in Wallonia and different virulotypes can be excreted by the same animal. Conversely the methodology followed gives no precise idea of the actual level of excretion since the hybridization and PCR were performed after enrichment in selective broth. Therefore multiple isolates belonging to the same virulotype might represent the same clone. Identification of the serogroups and comparison by Pulsed Field Gel Electrophoresis should help to clarify that point. Quantitative (q)PCR is today the best method to quantify bacterial excretion, but is more expensive. The results of the hybridization and PCR correspond to between 80 and 90%. Though the colony hybridization is still useful for large-scale surveillance it needs radioactive probes for highest sensitivity and is more time-consuming than PCR. Therefore the PCR should be the first routine choice if it can be automatized at large scale. Further steps are the confirmation of the PCR results of the 70 isolates with different hybridization and PCR results and the identification of the serogroups with the two pentaplex PCR and later with PCR for the other serogroups, to compare them with isolates from young diarrhoeic calves and from humans. [less ▲]

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See detailUse of NIR hyperspectral imaging to detect and quantify nodules on root system of associated crops
Eylenbosch, Damien ULg; Pierreux, Jérome ULg; Fernández Pierna, Juan Antonio et al

Poster (2016, September)

Winter wheat and peas cultivated in association and harvested when grains are dry show promising results in temperate areas. This association reach a good land equivalent ratio, reduce the nitrogen ... [more ▼]

Winter wheat and peas cultivated in association and harvested when grains are dry show promising results in temperate areas. This association reach a good land equivalent ratio, reduce the nitrogen fertilizer needs and obtain high quality harvest. These results are probably explained by the transfer of nitrogen from peas nodules to wheat roots. To test this hypothesis, quantification of nodules is required. Visual counting of nodules is time consuming and discrimination between the two kinds of roots is almost impossible. A new method is therefore needed. Near infrared hyperspectral imaging (NIR-HIS) was tested as a rapid method to quantify the amount of nodules and roots of each species in soil samples. [less ▲]

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See detailFunctional properties of extracellular polysaccharides produced by Enterobacter A47 grown on agro-food industry by-products
Antunes, Silvia; Freitas, Filomena; Alves, Vitor et al

Poster (2016, September)

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See detailSensitivity of the regional climate model ALARO-0 to land surface changes
Berckmans, Julie; Henrot, Alexandra-Jane ULg; Jacquemin, Ingrid ULg et al

Poster (2016, September)

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See detailDomestic tools, hafting, and the evolution of technology: The Upper Palaeolithic of Hohle Fels as a case study
Taipale, Noora ULg; Conard, Nicholas J.; Rots, Veerle ULg

Poster (2016, September)

Innovations relevant to human evolution often involve subsistence technology, which can affect the success of individual groups, and Homo sapiens in general. However, Palaeolithic technologies include ... [more ▼]

Innovations relevant to human evolution often involve subsistence technology, which can affect the success of individual groups, and Homo sapiens in general. However, Palaeolithic technologies include more than just hunting tools, and a proper understanding of hunter-gatherer ways of living requires knowledge of the organisation of diverse tasks and activities, including the manufacture and maintenance of tools and other equipment. One central aspect of technological evolution is the development of tool hafting [1, 2], which is not only restricted to hunting and gathering implements, but also affects so-called domestic tool categories. We present the results of an on-going project that focuses on hafting and use of stone tools in the Upper Palaeolithic through detailed functional analysis of selected assemblages from European key sites (Hohle Fels, Abri Pataud, Maisières-Canal), which have yielded rich lithic and organic assemblages from secure chronological contexts. Here the focus is on classic Upper Palaeolithic tool categories, such as endscrapers and burins, from the Gravettian and Magdalenian levels of the cave site Hohle Fels (Germany) [3, 4]. We suggest that domestic tools can offer a valuable source material, since for most of them, hafting is not a necessity as it is for spear and arrow tips. An increase in hafting implies an increase in time investment, which has implications for task organisation and specialisation. The Hohle Fels assemblage offers an interesting case study for temporal changes (or continuity) in the frequency and techniques of tool hafting. The projectile technology shows a clear shift from the Gravettian to the Magadalenian, marked by the introduction of a microlithic technology (backed bladelets). For other tool categories, the changes seem more subtle. Our goal is to characterise the tools used in manufacture and maintenance tasks, and to evaluate whether the Gravettian to Magdalenian transition witnesses changes in tool design and use that go beyond hunting equipment. The observed differences between tool classes and time periods are explained with a reference to details of tool use, such as the rate of edge wear development and stone tool exhaustion, as well as shifts in treatment of organic raw materials. The results suggest that domestic tools can aid in understanding long-term technological evolution, and create a baseline against which we can (re)assess the role of shifts observed in technologies that are more susceptible to morphological change, such as projectiles. References: [1] Rots, V., 2013. Insights into early Middle Palaeolithic tool use and hafting in Western Europe: The functional analysis of level IIa of the early Middle Palaeolithic site of Biache-Saint-Vaast (France). J. Archaeol. Sci. 40, 497–506. [2] Barham, L., 2013. From Hand to Handle: The First Industrial Revolution. Oxford University Press, Oxford. [3] Conard, N. J., Bolus, M., 2003. Radiocarbon dating the appearance of modern humans and timing of cultural innovations in Europe: New results and new challenges. J. Hum. Evol. 44, 331–371. [4] Taller, A., Bolus, M., Conard, N. J., 2014. The Magdalenian of Hohle Fels Cave and the Resettlement of the Swabian Jura after the LGM. In: Otte, M., Le Brun-Ricalens, F. (Eds.), Modes de contacts et de déplacements au Paléolithique eurasiatique: Actes du Colloque international de la commission 8 (Paléolithique supérieur) de l'UISPP, Université de Liège, 28–31 mai 2012. Centre National de Recherche Archéologique, Luxembourg. [less ▲]

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See detailA new method for identifying experimental and Palaeolithic hafting adhesives using GC×GC-HRTOFMS
Cnuts, Dries ULg; Perrault, Katelynn ULg; Dubois, Lena ULg et al

Poster (2016, September)

Hafting adhesives can be seen as an indication of the cognitive and technical capabilities of the manufacturers and therefore play a key role in the debate on human evolution [1], [2]. These adhesives are ... [more ▼]

Hafting adhesives can be seen as an indication of the cognitive and technical capabilities of the manufacturers and therefore play a key role in the debate on human evolution [1], [2]. These adhesives are mainly from plant origin (resins, gums or tar) and are often mixed with beeswax and other additives in order to make them less brittle. Archaeological evidence indicates that these adhesives were already in use in the Paleolithic from at least 120.000 years ago [3]. Discoveries for this period are however very rare and only become abundant from the Neolithic onwards [4]. Their longer exposure to biochemical alteration processes limits the chance of survival in the archaeological record. If they are present on Paleolithic stone tools, they appear often in such small quantities that they are challenging to identify by traditional gas chromatography – mass spectrometry (GC-MS) or even to remove them effectively from the stone tool. The destructive nature of traditional GC-MS analysis can damage these rare samples for other analyses. Our study aims to overcome this problem by using headspace solid phase microextraction (HS-SPME) for sample extraction and analysis by comprehensive two-dimensional gas chromatography –high-resolution time-of-flight mass spectrometry (GC×GC-HRTOFMS), which has the benefit of analyzing the volatile organic compound (VOC)s from the substance and it does not destroy the complete matrix of the adhesive. We present the results of a pilot study intended to examine the potential of this technique for analyzing Palaeolithic adhesives. The study involved (1) an examination of experimental compound adhesives (containing pine and spruce resin, acacia gum and birch tar; beeswax and additives like charcoal, flax or ochre), (2) a blind test on experimental samples to test the reliability of the method and to determine the minimal quantity necessary for analysis, and (3) the analysis of different Palaeolithic adhesives and of experimental samples of at least 15 years old. The analysis was done on extracted and non-extracted adhesives. A unique chromatographic fingerprint was obtained for all experimental adhesive samples. The VOC profile of these adhesives proved to be extremely complex and therefore benefitted significantly from multidimensional separation techniques. GC×GC-HRTOFMS provided an optimal chromatographic separation of adhesive components. HRTOFMS data was used in order to obtain high-resolution mass spectral data to contribute to compound identification. Our study demonstrates that GC×GC-HRTOFMS is a well suited method for identifying small quantities of compound adhesives with significant potential for Palaeolithic contexts. The additional sensitivity afforded by this technique in comparison to traditional GC-MS is a substantial benefit for these quantities. Furthermore, by only analyzing the VOCs of the adhesives, these rare archeological samples are not destroyed and can still be used for other types of analysis. [1] L. Wadley, ‘Compound-Adhesive Manufacture as a Behavioral Proxy for Complex Cognition in the Middle Stone Age’, Curr. Anthropol., vol. 51, no. s1, pp. S111–S119, Jun. 2010. [2] L. Barham, From Hand to Handle: The First Industrial Revolution. Oxford: Oxford University Press, 2013. [3] P. P. A. Mazza, F. Martini, B. Sala, M. Magi, M. P. Colombini, G. Giachi, F. Landucci, C. Lemorini, F. Modugno, and E. Ribechini, ‘A new Palaeolithic discovery: tar-hafted stone tools in a European Mid-Pleistocene bone-bearing bed’, J. Archaeol. Sci., vol. 33, no. 9, pp. 1310–1318, Sep. 2006. [4] M. Regert, ‘Investigating the history of prehistoric glues by gas chromatography-mass spectrometry.’, J. Sep. Sci., vol. 27, no. 3, pp. 244–54, Feb. 2004. [less ▲]

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See detailHow reliable are the models to study recent climate change ? A study of heat/cold waves and radiative fluxes trends over 1900-2010 using the model MAR in Belgium
Wyard, Coraline ULg; Fettweis, Xavier ULg; Scholzen, Chloé ULg

Poster (2016, August 29)

This research aims to assess the ability of the regional climate model MAR ("Modèle Atmosphérique Régional") to reconstruct the observed twentieth century climatology of extreme events and solar radiation ... [more ▼]

This research aims to assess the ability of the regional climate model MAR ("Modèle Atmosphérique Régional") to reconstruct the observed twentieth century climatology of extreme events and solar radiation in Belgium, as a necessary condition for reliable future projections. Simulations were performed by forcing MAR with several reanalyses: the ERA40/ERA-Interim, the ERA-20C and the NCEP/NCAR-v1. The results suggests that increasing air temperature would have generated decreasing relative humidity which would have lead to a decrease in cloudiness and an increase in solar downward radiation. This research illustrates the dependency between RCMs and their forcings. The forcing reanalyses can generate divergent trends while contrary to Global Climate Models (GCM), the reanalyses assimilate observations and are supposed to represent the same climate. [less ▲]

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See detailThe influence of background noise on vocal loading parameters in music theory teachers
Schiller, Isabel ULg; Morsomme, Dominique ULg; Sfez, Lou et al

Poster (2016, August 23)

Background: Music theory teachers, who teach rhythm, singing and other music-related skills and topics, depend greatly on a well-functioning voice. Unlike other schoolteachers, who primarily use their ... [more ▼]

Background: Music theory teachers, who teach rhythm, singing and other music-related skills and topics, depend greatly on a well-functioning voice. Unlike other schoolteachers, who primarily use their voice as a pedagogic tool, music theory teachers also use it as an instrument. Furthermore, they often engage in vocally demanding free-time activities requiring a singing voice. To date, few studies have specifically looked at the voice use of music theory teachers. This study aims (1) to measure the background noise level and the amount of vocal loading affecting this specific population, and (2) to describe the influence of background noise on vocal loading parameters. Methods: Thirteen French-speaking music theory teachers (9 females and 4 males) working in a music school were monitored for one workweek, using the VoxLog voice dosimeter (Sonvox). To investigate the professional and extra-professional environments, all subjects wore the dosimeter from early morning until the end of the day. The parameters analysed were background noise level, duration of phonation, sound pressure level (SPL) and fundamental frequency (F0) of voice. Results: Overall, the mean background noise level was 75.2 dB (SD=5.4). We measured higher background noise level at work (mean=78.2 dB, SD=5.8) than in the extra-professional environment (mean=72.2 dB, SD=5.2). As expected, a rise in background noise was accompanied by a significant rise in voice SPL in both males and females (r=.61, p<.001). A significant correlation between background noise and F0 was found in females (r=.41, p=.002), but not in males (r=.39, p=.055). Furthermore, our data exhibit a significant correlation between background noise and duration of phonation (r=.05, p<.001). Conclusion: Our data suggest, that in class, music theory teachers must cope with background noise levels that dramatically exceed the limit of 35 dB recommended by the WHO (Inserm, 2006). High background noise levels lead to an increase in voice SPL, a phenomenon known as the Lombard effect (Inserm, 2006). In female subjects, we also observed a rise in F0 further to high background noise. Like Ternström, Södersten, and Bohman’s (2002) study, our data indicate that high background noise levels increase the duration of phonation. In a noisy environment, subjects seem to prolong the voiced segments of speech to make themselves understood. In other words, high background noise levels result in higher vocal loading. In the long run, this may increase the risk of voice disorders such as hyperfunctional dysphonia or vocal fold pathologies consecutive to repeated microtrauma in music theory teachers. [less ▲]

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See detailOverhanging Constraints in Addivitive Manufacturing Using Two Different Tools
Bauduin, Simon ULg; Collet, Maxime ULg; Duysinx, Pierre ULg

Poster (2016, August 23)

Topology optimization is widely used as a design tool for advanced application in mechanical, aerospace and automotive industries. This technique offers an optimal distribution of a predetermined amount ... [more ▼]

Topology optimization is widely used as a design tool for advanced application in mechanical, aerospace and automotive industries. This technique offers an optimal distribution of a predetermined amount of material in a given design space. In the last years, a lot of efforts has been invested into the development of high performance methods such as homogenization, SIMP or BESO. However as the state of the art in manufacturing experiences evolution, a coupling between topology optimization and additive manufacturing is needed. Additive manufacturing has numerous advantages that fits the characteristics of topology optimized designs. It can manufacture highly complex design without high cost increase and furthermore continuous density material of the SIMP method could be manufactured by lattice structures. With all the opportunities given by the additive manufacturing the urge to bind the last one to topology optimization is heavily required. Specific constraints related to manufacturing issues have to be taken into account such as the need of supports structures to ensure a good heat evacuation during the manufacturing process, as well as to hold up overhanging section. Some researches have been done to try to include this constraint in the optimization problem such as Leary and al or Andrew T. Gaynor. However this work focuses on 2 different methods (projection scheme and mechanical approach) to tackle the overhanging problem and compare them . [less ▲]

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See detailA Comparison between four Techniques to Measure Cardiac Output
Pironet, Antoine ULg; Dauby, Pierre ULg; Chase, J. Geoffrey et al

Poster (2016, August 17)

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See detailCASTLE4D : The castle of Franchimont
Luczfalvy Jancsó, Andrea ULg; Hoffsummer, Patrick ULg; Billen, Roland ULg et al

Poster (2016, August 16)

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See detailTechniques et Méthodes nucléaires : Application de la Spectrométrie Gamma au Cameroun
Moyo N., Maurice; Kayo, Steve; Ngelem M., Eric J. et al

Poster (2016, August 04)

L’imaginaire populaire est de plus en plus pessimiste au sujet du mot nucléaire, utilisées selon eux, uniquement pour détruire, en cas de guerre. Une idée reçue, mais presque imposée par l'attitude des ... [more ▼]

L’imaginaire populaire est de plus en plus pessimiste au sujet du mot nucléaire, utilisées selon eux, uniquement pour détruire, en cas de guerre. Une idée reçue, mais presque imposée par l'attitude des pays développés qui donnent l'impression de recourir au nucléaire, le plus souvent, à des fins militaires. Pourtant, les applications du nucléaire sont utiles dans des domaines aussi variés que l'agriculture, la médecine, la détection des fuites dans les barrages, la surveillance de la qualité de l'air, des eaux, des sols, des matériaux de construction, des denrées alimentaires etc. Au Cameroun, l'utilisation des rayonnements ionisants (énergie nucléaire) est en pleine croissance. Ce, spécifiquement dans les domaines médical et industriel. La radioprotection est de plus en plus l’objet des recherches et des mesures au Cameroun. Des techniques d’analyse nucléaires sont également utilisées pour déterminer les risques radiologiques lies aux rayonnements naturels provenant de l’environnement. Dans ce cadre, l’unité de recherche « Physique Fondamentale » de l’université de Douala en collaboration avec l’ANRP (Agence Nationale de RadioProtection) utilise la spectrométrie gamma pour quantifier et qualifier l’exposition des camerounais aux rayonnements. Deux mémoires de master ont à cette occasion révéler le niveau d’exposition due aux rayonnements provenant du sol de l’Université de Douala d’une part et d’autre part l’exposition due aux matériaux de construction dans la ville de Douala. Les études sur les matériaux de construction (ciment utilisé dans la ville de Douala) ont permis d’observer que l’activité spécifique dans les échantillons de ciment varie d’un radionucléide à l’autre. L’estimation des paramètres radiologiques comme (l’activité du radium équivalent Raeq, l’indice du risque externe (Hex) et interne (Hin), le taux de dose absorbée dans l’air et la dose effective annuelle (AEDE) ont montré que l’utilisation comme matériaux de construction du ciment analysé était sans risque particulier. [less ▲]

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See detailCharacterization of Leptolyngbya and Phormidium diversity in Antarctic biotopes
Lara, Yannick ULg; Durieu, Benoit ULg; Borderie, Fabien et al

Poster (2016, August)

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See detailParcours migratoire et résilience : ressources et contraintes perçues par des réfugiés syriens et afghans
Vereshchagin, Anton ULg; Glowacz, Fabienne ULg

Poster (2016, August)

Les chiffres du Haut-Commissariat des Nations Unies pour les réfugiés indiquent une augmentation considérable du flux migratoire en Europe depuis 2010. Cette tendance s’explique notamment par une violence ... [more ▼]

Les chiffres du Haut-Commissariat des Nations Unies pour les réfugiés indiquent une augmentation considérable du flux migratoire en Europe depuis 2010. Cette tendance s’explique notamment par une violence persistante dans des pays tel que l’Afghanistan et l’évolution du conflit en Syrie. Dans le cadre d’une approche écosystémique, les contraintes perçues et rappelées par les sujets ayant « survécu » à la « traversée » migratoire et aux micro-traumatismes associés ont été mises en perspectives avec, les motivations liées à cette migration et les ressources perçues par les migrants. Notre étude de type exploratoire se base sur la rencontre avec 15 sujets âgés de 21 à 67 ans : 7 afghans et 8 syriens (13 hommes et 2 femmes) résidant dans des structures d'accueil pour demandeurs d'asile et hors structure, en Belgique. Des entretiens semi-structurés ont été menés en anglais et en russe et trois questionnaires ont été administrés : le locus de contrôle (1966), le sentiment d'auto-éfficacité (1997) et le soutien social perçu (1985). L'analyse des discours a mis en évidence différents types de motivations à la migration ne prédéterminant pas nécessairement la complexité du voyage qui apparait davantage dépendante des contextes situationnels, des opportunités et diverses contraintes. Par contre, les motivations semblent associées aux ressources perçues. Sur base de l’analyse du récit de la trajectoire, ainsi que des questionnaires, plusieurs facteurs de protection et de résilience ont été identifiés. Nous avons également proposé une nouvelle adaptation du modèle transthéorique de changement (DiClemente et Prochaska, 1982) au processus migratoire. [less ▲]

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See detailL’Hadrianus de Montserrat (P.Monts.Roca III, inv.162 → - 165 ↓) : une source nouvelle sur la Colonia Claudia Ara Agrippinensium ?
Berg, Tatiana ULg

Poster (2016, August)

Sixième partie du codex miscellaneus de Montserrat (P.Monts.Roca III, inv.162 → - 165 ↓ = MP3 2998.11 = LDAB 552, papyrus, 2e moitié IVe siècle), qui présente la particularité de contenir des textes à la ... [more ▼]

Sixième partie du codex miscellaneus de Montserrat (P.Monts.Roca III, inv.162 → - 165 ↓ = MP3 2998.11 = LDAB 552, papyrus, 2e moitié IVe siècle), qui présente la particularité de contenir des textes à la fois grecs et latins, profanes et chrétiens, tous écrits par la même main, le récit latin en prose Hadrianus relate notamment le passage de l’empereur Hadrien dans la ville de Cologne, dont il évoque des realia. Après les avoir répertoriées et avoir évalué leur authenticité, le poster met en évidence l’apport du texte à la connaissance de la Colonia Claudia Ara Agrippinensium et de son autel des Ubiens. [less ▲]

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See detailSarcoptic mange infection in red foxes (Vulpes vulpes) in Belgium
Volpe, Rosario ULg; Caron, Yannick ULg; Lesenfants, Christophe ULg et al

Poster (2016, August)

Eight cases of sarcoptic mange in foxes were reported during summer 2014 (n=3) and winter 2015 (n= 5) in Belgium. All animals came from the same restricted forest zones near urbanized areas and some of ... [more ▼]

Eight cases of sarcoptic mange in foxes were reported during summer 2014 (n=3) and winter 2015 (n= 5) in Belgium. All animals came from the same restricted forest zones near urbanized areas and some of them were discovered in private gardens (Forest District of Thuin, Hainaut, Belgium). Three of them were found dead, the others were shot for sanitary reasons. At necropsy, all of them presented large areas of alopecia and extensive skin lesions such as hyperkeratosis and suppurative lacerations. Animals presented poor body condition and some of them were severely emaciated (absence of visceral fat). Mange has profound influences on population since, if untreated, death follows in four to six months. In the present cases, cutaneous scrapings and histopathological examinations were systematically performed. Numerous parasites were observed in skin scrapings and marked dermo-epidermatitis with several parasites was observed in histopathological sections. These results suggest an outbreak of sarcoptic mange in red foxes in a limited area of the country. This is a first record for the country. Furthermore, investigations on lungs and digestive tracts performed on 3 of these foxes revealed they were also infected by parasites transmissible to pets and/or humans: Angiostrongylus vasorum was observed in the respiratory tract of one fox whereas Toxocara canis, Uncinaria stenocephala, Taenia spp. and Echinococcus multilocularis worms were detected in the digestive tract. In conclusion, urban foxes represent a source of parasites of public health and veterinary importance and foxes surveillance should be strengthened in these areas. [less ▲]

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See detailCyanobacterial diversity in soil crusts in the Sör Rondane Mountains
Namsaraev, Zorigto; Mano, Marie-José; Wilmotte, Annick ULg

Poster (2016, August)

Antarctica is the only continent that is dominated by microbial (cyanobacteria and algae) and lower plant (predominantly mosses and lichens) communities. Cyanobacteria are photosynthetic bacteria that ... [more ▼]

Antarctica is the only continent that is dominated by microbial (cyanobacteria and algae) and lower plant (predominantly mosses and lichens) communities. Cyanobacteria are photosynthetic bacteria that require solar light, liquid water, air and some mineral nutrients for growth. They serve as primary producers of organic matter in Antarctic ecosystems providing energy to other physiological groups of microorganisms and invertebrates. Cyanobacteria form macroscopically visible crusts or thin biofilms on the surface of soils and rocks, or occupy endolithic niches in Antarctic mountains. Mountains exposed above the ice sheet could have remained ice-free during glaciation maxima in Antarctica. They could serve as a refuge for terrestrial biodiversity and potential source for recolonization of surrounding habitats during glacier retreat. Cyanobacterial diversity in habitats located above 1 km a.s.l. was studied in several Antarctic locations. These include: the Vinson Massif in Ellsworth Mountains (2000-2500 m a.s.l.), Beacon (1176 m a.s.l.) and University Valleys (1700 m a.s.l.) in the "stable upland zone" of the McMurdo Dry Valleys (Southern Victoria Land) and the Sör Rondane Mountains (1370-1700 m a.s.l.) (Yergeau et al., 2007; Wood et al., 2008; Fernandez-Carazo et al., 2012). The goal of our work was to study cyanobacterial diversity of cyanobacteria in the Sör Rondane Mountains in the vicinity of Belgian Princess Elisabeth Station. Previous estimates of the diversity showed the presence of 10 morphotypes and 13 OTUs of cyanobacteria in 10 samples of biofilms and microbial crusts (Fernandez-Carazo et al., 2012). We performed a broader sampling and studied cyanobacterial diversity using DGGE with cyanospecific primers and microscopy. In 126 samples, we observed 15 morphotypes of cyanobacteria. 28 representative samples were selected for molecular analyses that revealed the presence of 28 OTUs (groups of 16S rRNA sequences sharing at least 97,5% sequence similarity). Comparison with other mountainous areas of Antarctica showed that the Sör Rondane Mountains harbor a significantly higher cyanobacterial diversity. Molecular analysis of the cyanobacterial diversity in Beacon Valley didn't show the presence of cyanobacteria (Wood et al., 2008), though a strain of Chroococcidiopsis sp. (CCMEE 134) was isolated from a sample collected there (Billi et al., 2011). A strain of Chroococcidiopsis sp. (CCMEE 171-A789-2) was also isolated from samples collected in University Valley (Cumbers & Rothschild, 2014). 5 OTUs of cyanobacteria were detected in samples collected in Ellsworth Mountains despite of the presumably harsher climate (78°31′S latitude compared to 77°49′S for Beacon Valley) and higher altitude (Yergeau et al., 2007). No reliable climate data are available for the discussed areas, except for the Sor Rondane Mountains. We propose that the higher diversity of cyanobacteria detected near the Princess Elisabeth Station could be explained by a more intensive sampling or by a more northern location of the area (72°0′S). [less ▲]

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See detailThe BCCM/ULC collection: a Biological Ressource Center to give access to the Antarctic cyanobacterial diversity
Wilmotte, Annick ULg; Renard, Marine ULg; Lara, Yannick ULg et al

Poster (2016, August)

On the Antarctic continent, Cyanobacteria represent the key primary producers and the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic ... [more ▼]

On the Antarctic continent, Cyanobacteria represent the key primary producers and the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts in terrestrial biotopes. They may present interesting features to survive freeze/thaw cycles, sea-sonally contrasted light intensities, high UV radiations, dessication and other stresses. The BCCM/ULC public collection funded by the Belgian Science Policy Office since 2011 aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.). It makes it available for researchers to study the taxonomy, evolu-tion, adaptations to harsh environmental conditions, and genomic make-up. It pres-ently includes 226 cyanobacterial strains, with 119 being of Antarctic origin (cata-logue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). The morphological identification shows that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. We present here the molecular datasets showing the diversity of the BCCM/ULC strains, studied on the basis of the 16S rRNA gene. A selection of strains was also characterized by sequencing of rpoC1, recA, and gyrA genes after amplification with newly designed primers. Our results mainly show that 25 OTUs included strains of Antarctic origin. Moreo-ver, strains identified as members of the genera Leptolyngbya or Phormidium ap-pear in several lineages. This supports the need to revise these polyphyletic genera with a simple filamentous morphology. A certain divergence of some Antarctic strains from related strains isolated from other regions can also be observed. It suggests that a portion of the Antarctic cyanobacterial flora may have evolved in-dependently from the cyanobacteria in other continents. [less ▲]

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See detailMicroalgae diversity along an Antarctic glacier forefield
Stelmach Pessi, Igor ULg; Rybalka, Natalia; Friedl, Thomas et al

Poster (2016, August)

Glacier retreat due to global warming has been observed in all the cryosphere [1], systematically exposing new terrestrial ecosystems that had previously been covered by ice. Primary succession, i.e. the ... [more ▼]

Glacier retreat due to global warming has been observed in all the cryosphere [1], systematically exposing new terrestrial ecosystems that had previously been covered by ice. Primary succession, i.e. the assembly of biological communities on newly exposed habitats and their change over time, can be studied along glacier forefields, where distance from the glacier terminus is used as a proxy for time since deglaciation [2]. The study of microbial succession is still at its infancy, but understanding the relationships between microbial communities and soil development will provide us with crucial knowledge on how they influence and respond to changes in environmental conditions. Here, we investigated the structure of microalgal communities along a deglaciation gradient in the forefield of Collins Glacier (Fildes Peninsula, King George Island, Maritime Antarctica). [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailProofing & Reduction Strategies Used by Emergency Residents to Manage Fatigue-related Risk
Berastegui, Pierre ULg; Ghuysen, Alexandre ULg; Nyssen, Anne-Sophie ULg

Poster (2016, July 29)

Fatigue and sleep loss are typically associated with psychomotor and cognitive impairment resulting in poorer task performance. Most of these studies are conducted in controlled settings and involve the ... [more ▼]

Fatigue and sleep loss are typically associated with psychomotor and cognitive impairment resulting in poorer task performance. Most of these studies are conducted in controlled settings and involve the completion of experimental tasks. Only a few field studies involving exhausted residents have been conducted over the past decade, and they yielded to contradictory results (Ellman et al., 2004). One of the key factors that could be involved in the non-linear relationship between fatigue and performance in specific work context reside in the mobilization of Fatigue Proofing Strategies. FPS are adaptive and protective risk-reduction behaviors that improve the resilience of a system of work (Dawson et al., 2012). In this study, we aimed to identify and classify proofing strategies mobilized by EMS residents using an inductive content analysis approach. EMS residents reported a range of strategies for reducing subjective level of sleepiness (reduction strategies, n=15) or managing its consequences (proofing strategies, n=17). Content analysis yielded to three sub-categories of proofing strategies: Behavioral Compensation (n=8), Error’s Opportunity Reduction (n=5) and Error’s Consequences Mitigation (n=4). Our results show that EMS residents use both types of strategies although none of the proofing strategies were part of their training program. Despite the current informal use, there is significant potential for implementation of more formal processes. [less ▲]

Detailed reference viewed: 49 (7 ULg)
Full Text
Peer Reviewed
See detailAharonov-Bohm oscillations of bosonic matter-wave beams in the presence of disorder and interaction
Chrétien, Renaud ULg; Dujardin, Julien; Petitjean, Cyril ULg et al

Poster (2016, July 27)

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that ... [more ▼]

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that is outcoupled from a magnetic trap into a 1D waveguide which is made of two semi-infinite leads that join a ring geometry exposed to a synthetic magnetic flux φ. We specifically investigate the effects both of a disorder potential and of a small atom-atom contact interaction strength on the AB oscillations. The main numerical tools that we use for this purpose are a mean-field Gross-Pitaevskii (GP) description and the truncated Wigner (tW) method. We find that a correlated disorder suppress the AB oscillations leaving thereby place to Aronov-Al’tshuler-Spivak (AAS) oscillations. The competition between disorder and interaction leads to a peak inversion at Φ = π, that is a signature of a coherent backscattering (CBS) peak inversion. This is confirmed by truncated Wigner simulations. [less ▲]

Detailed reference viewed: 32 (4 ULg)
See detailBiofilm fermentation for the production of insect pathogenic fungi
Bawin, Thomas ULg; Francis, Frédéric ULg; Delvigne, Frank ULg

Poster (2016, July 27)

Currently, integrated pest management that aims to reduce the use of synthetic insecticides by considering all appropriate alternative methods, is promoted. In that context, insect pathogenic fungi could ... [more ▼]

Currently, integrated pest management that aims to reduce the use of synthetic insecticides by considering all appropriate alternative methods, is promoted. In that context, insect pathogenic fungi could be developed as biopesticides in two ways: spores but also metabolites that are recognized as virulence factors. Targeting adequate fermentation method is an important requirement to obtain fungal products (i.e. spores, enzymes and metabolites) of biotechnological interest. The 'Microbial Processes and Interactions' laboratory has developed an innovative fermentation technology (referred to as ‘biofilm fermentation’) involving the growth of fungal biomass on inert supports that are immersed in a liquid nutrient medium. Confining fungal biomass on immersed inert structures provides a hybrid production system aiming to keep a solid-state physiology for sporulation and secretion of metabolites while controlling fermentation parameters. This research project proposes to transfer this technology to culture insect pathogenic fungi, such as Metarhizium anisopliae, for the production of spores and insecticidal metabolites. Development stages include the qualitative and quantitative characterization of its impact on insecticidal products as well as scale-up to industrial level. [less ▲]

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Peer Reviewed
See detailQuantitative characterization and calibration of salt water intrusion models with electrical resistivity tomography
Beaujean, Jean; Hermans, Thomas ULg; Vandenbohede, Alexander et al

Poster (2016, July 26)

Groundwater quality and coastal ecosystems in coastal areas are among the most vulnerable as they are threatened by excessive groundwater withdrawals, sea level rise and storm events potentially leading ... [more ▼]

Groundwater quality and coastal ecosystems in coastal areas are among the most vulnerable as they are threatened by excessive groundwater withdrawals, sea level rise and storm events potentially leading to salt water intrusions or infiltration into fresh water aquifers. The environmental protection and sustainable management of these groundwater resources often involves the development and calibration of a groundwater model subsequently used to forecast the total dissolved solid content (TDS). However, groundwater models are often built based on a limited number of sparse data due to borehole availability. Geophysical methods can provide spatially and temporally distributed data for hydrogeological modeling at relatively limited costs. In particular, electrical resistivity tomography (ERT) is very sensitive to the conductivity of pore water which is directly linked to the TDS content. The method is therefore well-suited for the monitoring of salt water intrusions. However, the inversion of ERT data involves a regularization process so that the resulting tomogram is only an estimate of the true resistivity distribution, suffering from smoothing and varying resolution. In many cases, the interpretation of ERT remains qualitative and skewed. In this contribution, we propose two different methods to improve the information content that can be extracted from ERT data. First, we show with a field example from Belgium how alternative regularization methods can be developed to integrate independent information into the inversion process of ERT. This enabled us to obtain a resistivity distribution much closer to the one observed in validation boreholes. Then, a site-specific petrophysical relationship is used to derive the TDS content of the aquifer from ERT tomograms. This can be directly used as input in the calibration process of a hydrogeological model. We also show how it is possible to counterbalance the effect of resolution loss with depth for surface ERT by filtering the results relative to their sensitivity. We show that this filtering is mandatory to use the ERT-derived information for calibrating a hydrogeological model. In a second example, we show how a fully coupled inversion approach can be used to directly invert geophysical data together with hydrogeological data for the calibration of hydrogeological models. At each iteration of the calibration, the simulated TDS content is transformed in a resistivity distribution using a parameterized petrophysical relationship and forward geophysical modeling yields the geophysical response. We show that this approach enables to better estimate the hydrogeological parameters of the simulated coastal aquifer than with an uncoupled approach if the conceptual model is sufficiently representative. With those two examples, we demonstrate the usefulness of ERT in the monitoring of salt water intrusions, both qualitatively to identify most vulnerable zones and quantitatively to estimate ERT-derived TDS contents or geophysical data and calibrate hydrogeological models. An innovative approach may consist in a conjunctive use of filtered geophysically-derived and geophysical data within the coupled hydrogeophysical inversion framework. Such an uncoupled-coupled approach based on a resolution threshold approach may offer a promising developing trend in hydrogeophysical inversion. [less ▲]

Detailed reference viewed: 77 (4 ULg)
See detailImpacts of entomopathogenic fungi on biology and behaviour of the invasive Brown Marmorated Stink Bug (Hemiptera, Pentatomidae)
Fingu Mabola, Junior Corneille ULg; Serteyn, Laurent ULg; Bawin, Thomas ULg et al

Poster (2016, July 25)

Halyomorpha halys St˚al (Hemiptera, Pentatomidae) is an invasive stink bug coming from Eastern Asia. Besides causing important yields losses in orchards, crop and vegetable fields, it overwinters inside ... [more ▼]

Halyomorpha halys St˚al (Hemiptera, Pentatomidae) is an invasive stink bug coming from Eastern Asia. Besides causing important yields losses in orchards, crop and vegetable fields, it overwinters inside houses as agglomerates of dozens. In Europe, this Brown Marmorated Stink Bug (BMSB) was accidentally introduced in Switzerland in 2007 and has been later observed in surrounding countries (France, Italy, Germany). Based on the current US situation, we can reasonably fear that BMSB will have colonized most of Europe countries in less than a decade. If we already discern a few fungus strains that are efficient against BMSB, very little is known about their actual impact on the insect itself. So we investigated the in- fluence of a fungal infection on insect development parameters and behaviour. Olfactometry was settled to investigate the dispersion and aggregation trends, while electropenetography was used to assess the feeding behaviour. Our results will be discussed to present changes according to healthy/fungus-infected status, in relation to potential biological control for that pest. [less ▲]

Detailed reference viewed: 57 (11 ULg)