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See detailThermal and structural behavior of two lauric fats compared to AMF in bulk and oil-in-water emulsion states
Petrut, Raul Flaviu ULg; Anihouvi, Prudent Placide; Blecker, Christophe ULg et al

Poster (2015, March 15)

In a previous study the thermal and polymorphic behavior of four different industrial lauric fats which are sold under the same commercial description, was compared (1). According to the findings the four ... [more ▼]

In a previous study the thermal and polymorphic behavior of four different industrial lauric fats which are sold under the same commercial description, was compared (1). According to the findings the four fats were split in two groups based on the similarities found at polymorphic level. In the present paper two of these industrial lauric fats (F1 and F2, one from each group) were incorporated into oil -in -water emulsions. The objective of this study was to point out the differences in the crystallization and polymorphic behavior between bulk and emulsified fat. Moreover anhydrous milk fat (AMF) was used in the same way for comparison. For that purpose, the fats were investigated for their thermal and polymorphic behavior by differential scanning calorimetry (DSC) and X-ray diffraction (XRD), both in bulk and emulsified state. The DSC and X -Ray investigations made possible the observation of the differences in the crystallization behavior and kinetics that occurred for those fats in the bulk and emulsified state. 1. Anihouvi, P.P., C. Blecker, A. Dombree, S. Danthine, Comparative Study of Thermal and Structural Behavior of Four Industrial Lauric Fats, Food Bioprocess Technol. 6:3381-3391 (2013) [less ▲]

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See detailPhysicochemical properties and thermal behaviour of African wild mango (Irvingia gabonensis) seed oil.
Yamoneka, Juste; Malumba Kamba, Paul ULg; Blecker, Christophe ULg et al

Poster (2015, March 15)

Plants belonging Dacryodes and lrvingia genus have great economic importance in Central and West Africa. lrvingia gabonensis (IG) produces a seed rich in fats, traditionally used as a soup thickener ... [more ▼]

Plants belonging Dacryodes and lrvingia genus have great economic importance in Central and West Africa. lrvingia gabonensis (IG) produces a seed rich in fats, traditionally used as a soup thickener. Fruit of Dacryodes edulis (DS) contains a pulp how are also rich in fat. Its seed constitute a big waste problem in towns and village remains not exploitable by local people however that may be valued as a resource for various components. Some study shows that this seed contented a proportion of oils that would be an alternative in the substitution of diesel relevant to their physicochemical properties. Despite the potential of two different oils, theirs scientific data still lack. In this work, thermo-physical behaviour of those two oils is compared in the aim to improving knowledge about properties of those oils that will promote their valorisation in industry scale. The result of this investigation showed that IGO have a highest proportion of oil content with 72.38% compared to DSO (14.64%). IGO is rich in myristic (49.8%) and lauric fatty acid (37%) but DSO sample was found to possess a higher proportion of linoleic acid (35.13%), palmitic (32.67%), oleic (17.70%) but lower proportion of stearic (9.97%). SFC of IGO was the highest at all temperatures. IGO is solid at room temperature with a complete melting at 40°C but DSO is liquid at room temperature. The polymorphism profile of IGO and DSO shows the 13' form stable during heating and a transition to liquid state without passage at β form. [less ▲]

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See detailPhysicochemical properties and thermal behaviour of African wild mango (Irvingia gabonensis) seed oil.
Yamoneka, Juste; Malumba Kamba, Paul ULg; Blecker, Christophe ULg et al

Poster (2015, March 15)

African wild mango (lrvingia gabonensis) is now recognized for its numerous food and medicinal uses. This plant produces seeds rich in fat, which is traditionally used as a soup thickener. in the present ... [more ▼]

African wild mango (lrvingia gabonensis) is now recognized for its numerous food and medicinal uses. This plant produces seeds rich in fat, which is traditionally used as a soup thickener. in the present study, the proximate composition of seeds from lrvingia gabonensis is reported. The oil from the seeds was then solvent extracted; its fatty acid (FA) and triacylglycerol (TG) profiles were determined. Besides this chemical characterization, the melting and crystallization behaviour of the extracted fat was studied by pNMR, DSC and X-ray diffraction. The result of this investigation showed that the seeds from lrvingia gabonensis (IG) represent an important source of lipids (69-75%), rich in myristic (49.8%) and lauric acid (37%). This fat can be classified among "lauric fats". IG oil presents a good oxidative stability due to its low content in unsaturated fatty acid. The melting profiles of IG oil indicates that a high amount of fat remains solid at temperatures up to 30°C; the complete melting was detected at around 40C. The most stable polymorph of this fat is the β'1 -form. [less ▲]

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See detailMise au point du fractionnement par debranning des tissus périphériques du blé tendre (Triticum aestivum) et du blé dur (Triticum Durum)
Roiseux, Olivier; Ben Hamouda, Mohamed Salah; Olive, Gilles et al

Poster (2015, March 15)

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Depuis longtemps, le blé est broyé pour donner soit la farine dans le cas des blés tendres, soit de la semoule dans le cas ... [more ▼]

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Depuis longtemps, le blé est broyé pour donner soit la farine dans le cas des blés tendres, soit de la semoule dans le cas des blés durs, ceci correspond en fait à la majorité de l’albumen. Le son de blé est un coproduit de cette transformation et consiste en un empilement de différents tissus : péricarpe externe et interne, testa, épiderme et aleurone. A l’heure actuelle, la valorisation du son de blé est limitée malgré son potentiel nutritionnel. Un fractionnement spécifique de ces tissus, conduisant à la production de fractions ciblées à haute valeur ajoutée, permettrait de mieux exploiter ce potentiel. Parmi les méthodes de fractionnement par voie sèche, le debranning permet le retrait séquentiel des différentes couches extérieures du blé mettant en jeu l’abrasion contre une surface abrasive et la friction des grains entre eux. L’étude a porté sur deux variétés de blé tendre (Julius et Sahara) et deux de blé dur (Karim et Razzek). En raison de différences physiques comme la dureté ou la taille et la forme des grains, des cycles de traitement adéquats pour chacun des variétés dans le but de mettre en évidence leurs différences comportementales. Les quatre fractions (F1 ; F2 ; F3 ; F4) obtenues ont alors été analysées statistiquement (moyenne, coefficient de variation, minimum et maximum, intervalles de confiance) afin de juger de la conformité de ces fractions en termes de pourcentage massique par rapport au but désiré. Les moyennes et intervalles de confiance montrent que les fractions développées sont conformes aux couches histologiques d’un point de vue massique, vis à vis des valeurs « objectifs » fixées par rapport à la littérature scientifique. [less ▲]

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See detailAnhydrous Milk Fat enrichment with 13C-­triacylglycerol tracers: effects on thermal and structural behavior.
Danthine, Sabine ULg; Vors, Cécile; Blecker, Christophe ULg et al

Poster (2015, March 15)

Dietary lipids are incorporated in food products under different types of structures, e.g., as dispersed lipid droplets (in oil -in -water emulsions, like creams) or as a continuous lipid phase (in water ... [more ▼]

Dietary lipids are incorporated in food products under different types of structures, e.g., as dispersed lipid droplets (in oil -in -water emulsions, like creams) or as a continuous lipid phase (in water -in -oil emulsions, like butter for example). The crystallization, melting behavior and polymorphic stability of fats are determined by the behavior of the TAGs they contain. In clinical studies, there is a need to add some 13C TAGs as tracers to the ingested fats in order to track their metabolic fate. However, this procedure could modify the physicochemical properties of the fat. The present study was conducted in the framework of a clinical trial aiming at highlighting the effect of the physical structure of a fat (droplets in 0/W emulsion or bulk) in a meal on the absorption, chylomicron transport and further metabolic handling of dietary fatty acids (1). We therefore monitored the thermal and polymorphic behavior of anhydrous milk fat (AMF) enriched in tracers (a mixture of tripalmitin, triolein and tricaprylin; at 2 different concentrations: 1.5 and 5.7 wt%) using DSC and XRD and further compared it to the native AMF. The addition of 13C TAGs modified the AMF melting profile, especially at high concentration. The enriched AMF was completely melted at around 37°C, i.e. close to the body temperature. However, under some conditions, the AMF enriched in high 13C TAGs concentration remained crystallized at 37°C. Similar trends were observed in both systems (bulk vs emulsified). Moreover, AMF polymorphic behavior was also modified upon tracer addition. While only β’ form was observed in the native AMF, the 13 -form was detected in the AMF containing high 13C TAGs concentration. Importantly, low concentration of tracers should not have high impact on human digestive physiology. However more attention should be paid to physicochemical structure when high concentrations are added. (1) Vors et al. 2013. Modulating absorption and postprandial handling of dietary fatty acids by structuring fat in the meal: a randomized cross -over clinical trial. Am J Clin Nutr, 97(1): 23-36. [less ▲]

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See detailFood Compounds from Meadow Grasshoppers
Paul, Aman ULg; Frederich, Michel ULg; Uyttenbroeck, Roel ULg et al

Poster (2015, March 15)

Eating insects as food, particularly grasshopper is practiced in many cultures throughout the world. Meadow grasshopper (Chorthippus parallelus) is commonly found in Europe and some part of Asia. It is ... [more ▼]

Eating insects as food, particularly grasshopper is practiced in many cultures throughout the world. Meadow grasshopper (Chorthippus parallelus) is commonly found in Europe and some part of Asia. It is already known that grasshoppers of Chorthippus species are consumed as food in countries such as Thailand. With the aim of evaluating the nutritional potential of this insect species the proximate nutritional composition of grasshoppers caught from the local fields was realized. Besides this, the fatty acid profile of extracted lipids, amino acid profile and mineral composition of the insect was also revealed. Results suggest that meadow grasshopper is an excellent source of protein and essential amino acids. Lipids extracted from the grasshopper have an interesting fatty acid composition. Also the grasshopper contains some minerals that are important for body. With such protein content, amino acid profile, fatty acid profile of the lipids and mineral content this grasshopper species could present an interesting alternate to conventional protein sources. [less ▲]

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See detailCaractérisation physico-chimique et structurale de la poudre et des ulvanes de l'algue ulva lactuca
Guidara, Mariem; Yaich, Hela; Garna, Haikel et al

Poster (2015, March 15)

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See detailGenetic Control of Flowering Time in Arabidopsis: an Interactive Database
Bouché, Frédéric ULg; Lobet, Guillaume ULg; Tocquin, Pierre ULg et al

Poster (2015, March 12)

The transition to flowering is an essential step of the plant life cycle that is tightly controlled by both endogenous and environmental cues. Its regulation is extremely complex and involves hundreds of ... [more ▼]

The transition to flowering is an essential step of the plant life cycle that is tightly controlled by both endogenous and environmental cues. Its regulation is extremely complex and involves hundreds of genes that are part of highly interconnected pathways. Our knowledge of the molecular mechanisms governing the floral induction of Arabidopsis thaliana increases quickly and a significant number of reviews are published every year on this topic. However, most of them focus on a single pathway without highlighting the interconnections existing between them. Furthermore, those reviews become rapidly outdated, since our comprehension of the genetic control of flowering time evolves continuously. Hence, we believe that the current landscape of flowering time research in Arabidopsis misses an exhaustive repository of the genes involved in the control of flowering and their regulatory pathways. Here, we present a new interactive resource built around a curated database of the flowering time genes that brings together multiple pieces of information such as their function, the flowering time phenotype of mutants and overexpressing lines, the related key publications, etc. Our website thus gives access to a curated and exhaustive list of the genes involved in the regulation of flowering time in Arabidopsis as well as the regulatory pathways controlling their expression. Because of its flexibility, the database is highly dynamic and will be periodically updated with the future breakthroughs in this domain. [less ▲]

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See detailConcomitant nodal involvement by Langerhans Cell Histiocytosis and Hodgkin Lymphoma
Geurten, Claire ULg; THIRY, Albert ULg; Jamblin, Paul et al

Poster (2015, March 12)

Introduction : Langerhans cell histiocytosis is defined as a clonal neoplastic proliferation of myeloid dendritic cells that upon activation migrate from the mucosal to lymph nodes. Definitive diagnosis ... [more ▼]

Introduction : Langerhans cell histiocytosis is defined as a clonal neoplastic proliferation of myeloid dendritic cells that upon activation migrate from the mucosal to lymph nodes. Definitive diagnosis is made by anatomo-pathological and immunohistochemical analysis. Langerhans cell histiocytosis is rarely, yet not exceptionally, found coexisting with other malignant neoplasms, suggesting it might arise in reaction to the cytokinic secretion of malignant cells. Case : We report the case of a 10-year-old female presenting with an isolated laterocervical lymphadenopathy and a mild general condition alteration tracing back to two months earlier. Nodal biopsy was performed and revealed concomitant involvement by Langerhans cell histiocytosis and Hodgkin lymphoma. Treatment of lymphoma led to the disappearance of the whole symptomatology. Discussion : Literature beholds reports of 30 cases of the simultaneous occurence of Hodgkin lymphoma with Langerhans cell histiocytosis, which is more than fortuitous regarding the low incidence of both diseases. A common etiology could explain such an association, but it might also be possible that background inflammatory cells of Hodgkin lymphoma stimulate the proliferation of Langerhans cells, making it a reactive process when occurring simultaneously with other neoplasms. Clinicians should thus be aware of the possibility of this association and carefully exclude any other life-threatening malignant proliferation when confronted to apparently isolated Langerhans cell histiocytosis. [less ▲]

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See detailCerebellar hemorrhage : a rare condition in the term infant
MERINDOL, Ninon; BROUX, Isabelle ULg; DECORTIS, Thierry et al

Poster (2015, March 12)

Cerebellar hemorrhage is a rare condition in full-term newborns. Early diagnosis based on the identification of risk factors, particular clinical signs and correct medical imaging is primordial to ... [more ▼]

Cerebellar hemorrhage is a rare condition in full-term newborns. Early diagnosis based on the identification of risk factors, particular clinical signs and correct medical imaging is primordial to optimize the immediate treatment and to assess the long term prognosis. [less ▲]

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See detail“Rhizoponics”: a novel hydroponic rhizotron for root system analyses on mature Arabidopsis thaliana plants
Mathieu, Laura ULg; Lobet, Guillaume ULg; Tocquin, Pierre ULg et al

Poster (2015, March 10)

Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system ... [more ▼]

Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system architecture therefore remains a challenge. In the model plant Arabidopsis thaliana, in vitro culture remains the easiest and preferred method to study root development, which limits the analyses to young seedlings. We present here an innovative design of hydroponic rhizotron (rhizoponics) designed for the root system analysis of adult plants of Arabidopsis thaliana. [less ▲]

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See detailA new case of microdeletion 14q32.3
Uwineza, Annette ULg; BULK, Saskia ULg; CABERG, Jean-Hubert ULg et al

Poster (2015, March 06)

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See detailA tale of two anomalies. A paternal duplication and a maternal deletion of 15q13
BULK, Saskia ULg; Decortis, Thierry ULg; Rondia, G et al

Poster (2015, March 06)

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See detailTowards a yeast reference interactome
Desbuleux, Alice ULg

Poster (2015, March)

Despite an increasing number of interactomic datasets already available for model organisms and humans, many aspects remain contradictory, debatable or unclear due to the lack of complete high-quality ... [more ▼]

Despite an increasing number of interactomic datasets already available for model organisms and humans, many aspects remain contradictory, debatable or unclear due to the lack of complete high-quality networks. It has, for instance, been proposed that macromolecule connectivity in interactome maps reflects functional importance, functional relatedness, pleiotropy, implication in diseases, and other important biological characteristics. The most notorious example of such relationships concerns so-called essential genes believed to correspond to highly connected hubs that are critical to network integrity. Such claims have led to debate in the literature because connectivity could also be explained by bias and uneven coverage of the interactome space. To provide fresh insight into these questions, we produced a new, systematic interactome map for S. cerevisiae, organism for which a plethora of systematic interactomic and functional data is available. This alternative view of the interactome network was generated by modifying our screening pipeline based on our empirically-controlled framework. Using a new high-quality ORFeome collection and a new assay version, we systematically performed three replicate yeast-two hybrid screens. This produced a map of 1,200 protein-protein interactions, which, while of similar size as previously published interactome maps, covers the entire proteome without bias. These interactions were subsequently successfully validated using an orthogonal protein complementation assay based on a split Gaussia princeps luciferase. The latest analyses of this new map and progress towards generating a first Yeast Reference Interactome map will be presented. [less ▲]

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See detailCortical responses to transcranial magnetic stimulation during Non-Rapid Eye Movement Sleep
Gosseries, Olivia ULg; Jaakko, Nieminen; Siclari, Francesca et al

Poster (2015, March)

Transcranial magnetic stimulation coupled to high-density electroencephalography (TMS-EEG) allows for directly and non-invasively stimulating the brain and recording the subsequent cortical response ... [more ▼]

Transcranial magnetic stimulation coupled to high-density electroencephalography (TMS-EEG) allows for directly and non-invasively stimulating the brain and recording the subsequent cortical response. Previous TMS-EEG studies have shown clear-cut differences between conscious and unconscious conditions. When subjects are unconscious, as in non-rapid eye movement (NREM) sleep early in the night (stage N3), anesthesia or coma, TMS typically triggers a stereotypical and local slow-wave response. When they are conscious, as in normal wakefulness and rapid eye movement sleep (REM) sleep, brain responses to TMS are long-lasting, widespread, complex, and differentiated. In this study, we performed TMS-EEG during NREM sleep in stage 2 (N2) and N3 on 7 healthy participants. Brain activity was recorded using a 60-channel TMS-compatible EEG and single-pulse TMS was applied (up to 285 pulses per session) on the superior parietal cortex. After each TMS session (5 to 15 sessions per night), subjects were awakened to ask for a dream report. TMS-EEG responses were analyzed using the global mean field amplitude (GMFA), the perturbational complexity index (PCI), as well as time-frequency measures. Our results show that the TMS-EEG response during N3 is larger and slower than during N2 sleep. The GMFA is higher and PCI is lower in N3 as compared to N2 sleep. Moreover, N3 recordings showed activation at lower frequency bands after TMS as compared to N2 sleep. Additionally, TMS-EEG responses vary depending on whether subjects do or do not report a dream. TMS-EEG might provide valuable information for characterizing neurophysiological fluctuations and levels of consciousness within NREM sleep. [less ▲]

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See detailEtude GEROX : Evaluation du statut de stress oxydant
CHRISTELBACH, SOPHIE; RICOUR, Céline ULg; Dardenne, Nadia ULg et al

Poster (2015, March)

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See detailCan the exploration of left space be induced implicitly in unilateral neglect?
Wansard, Murielle ULg; Bartolomeo, Paolo; Vanderaspoilden, Valérie et al

Poster (2015, March)

The purpose of the present study was to explore the ability of neglect patients to detect and exploit the predictive value of a cue to respond more quickly and accurately to targets on their ... [more ▼]

The purpose of the present study was to explore the ability of neglect patients to detect and exploit the predictive value of a cue to respond more quickly and accurately to targets on their contralesional side in a Posner spatial cueing task. The majority of the cues (i.e. 80%) were invalid, indicating that the target would appear on the opposite side, although patients were not informed of this bias. Our results demonstrate that some neglect patients were able to extract the cue’s predictability and use it to orient faster toward the left. This cueing effect was present even in patients who were subsequently unable to describe the predictive character of the cues, and thus was not modulated by reportable awareness of the cue-target relation. [less ▲]

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See detailAutoimmune thyroid diseases in early childhood three case reports
GUFFINS, A; HARVENGT, Julie ULg; LUYCKX, F et al

Poster (2015, March)

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See detailIsolation of cultivable viruses from Antarctic wintertime sea ice
Luhtanen, A.-M.; Bamford, D.; De Jong, J. et al

Poster (2015, March)

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See detailELICITOR SCREENING TO PROTECT WINTER WHEAT AGAINST ZYMOSEPTORIA TRITICI BLOTCH
Le Mire, Géraldine ULg; Siah, Ali; Jijakli, Haissam ULg

Poster (2015, March)

Plant protection strategies are strongly focused today on the development of alternative methods in order to complete or replace conventional chemical inputs. Elicitors of biological origin are ... [more ▼]

Plant protection strategies are strongly focused today on the development of alternative methods in order to complete or replace conventional chemical inputs. Elicitors of biological origin are increasingly considered as a promising tool as biocontrol agents. They offer the possibility, through the induction of plant natural defences, to preventively protect plants against a large spectra of diseases. Multiple elicitors have already been identified since their first discovery in the late 70s, but few research has been focused on crop pathogens although such diseases can strongly impact both yield and grain quality. We describe hereby a method of elicitor screening to protect wheat against Zymoseptoria tritici. Focus is made on the importance of a good disease infection protocol in order to achieve screening tests. [less ▲]

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See detailCharacterization of Venom Peptides using Microfluidic Separation Techniques coupled to Mass Spectrometry (LC-MS and CE-MS)
Degueldre, Michel ULg; Delvaux, Cédric ULg; Far, Johann ULg et al

Poster (2015, March)

More than the half of the principal sub-kingdoms of the animal world contains species that produce venom whose purposes are to immobilize, kill and pre-digest the preys. These venoms represent an ... [more ▼]

More than the half of the principal sub-kingdoms of the animal world contains species that produce venom whose purposes are to immobilize, kill and pre-digest the preys. These venoms represent an exceptionally rich source of various biologically active peptides, both in their structures and their effects, which are more and more useful for human being1. Yet, the total characterization of such complex samples require advanced analytical techniques mainly due to the complexity of the sample (hundreds of compounds), the limited quantities usually available and the presence of numerous PTMs, especially disulfide bridges and specific folding. Here we present a method that combines LC and CE separation techniques coupled to mass spectrometry (MS) to characterize the peptide composition of the snake venom Naja atra. The characterization will not only focus on the toxin sequencing (LC-MS and LC-MS/MS), but will also aim at analyzing the folding of the toxins (CE-MS). To this end, native and reduced/alkylated toxins will be analyzed by both techniques. Final result targets the determination of the global hydrophobic pattern and native tridimensional folding of these strongly reticulated peptides. (1) Richard J. Lewis & Maria L. Garcia, Therapeutic potential of venom peptides. Nature Reviews Drug Discovery 2003, 2, 790-802. [less ▲]

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See detailStrain localization modelling around an underground gallery in Mol with an analysis of the contact pressure on the lining
Salehnia, Fatemeh ULg; Dizier, Arnaud; Li, Xiang Ling et al

Poster (2015, March)

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See detailPhotosynthesis-irradiance response curves revealed active sympagic communities in the Weddell Sea Winter, 2013
Rintala, J.-M.; Luhtanen, A.-M.; Enberg, S. et al

Poster (2015, March)

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See detailDifferential membrane marker expression in adult rodent bone marrow mesenchymal and neural crest stem cells.
Wislet, Sabine ULg; Coste, Cécile ULg; Neirinckx, Virginie ULg et al

Poster (2015, March)

Bone marrow stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy. With regards to recent findings ... [more ▼]

Bone marrow stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy. With regards to recent findings, adult mesenchymal stem cells (MSC) are commonly assimilated to neural crest stem cells (NCSC), both isolated from adult bone marrow. The objective of this study was therefore to highlight significant differences for membrane markers between those two cell types. Using the minimal criteria for defining multipotent mesenchymal stromal cells as previously described by The International Society for Cellular Therapy, we were quite surprised that no significant difference could discriminate MSC from NCSC. To define new markers, we first performed a microarray comparison. Based on those results, we validated selected targets by RT-PCR, then by immunocytochemistry. In parallel, we observed that NCSC had the unique property (compared to MSC) to grow as spheres, which could also be used as a purification protocol for NCSC from adult bone marrow. Altogether, we demonstrated that P75NTR was the most significant discriminating marker between MSC and NCSC, isolated from mouse adult bone marrow, which could be used as selecting marker in an enrichment protocol. Sphere formation could then be used as a purification protocol for NCSC. [less ▲]

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See detailAlignments of quasar axes with large-scale structures
Hutsemekers, Damien ULg; Braibant, Lorraine ULg; Pelgrims, Vincent ULg et al

Poster (2015, March)

Based on measurements of optical linear polarization of quasars belonging to large groups at redshift z ~ 1.3, we found that quasar spin axes are likely parallel to their host large-scale structures ... [more ▼]

Based on measurements of optical linear polarization of quasars belonging to large groups at redshift z ~ 1.3, we found that quasar spin axes are likely parallel to their host large-scale structures (Hutsemékers et al. 2014). These observations can constrain models of the coevolution of AGN, galaxies and large-scale structures. [less ▲]

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See detailEffects of transcranial magnetic stimulation coil orientation and pulse width on short-latency afferent inhibition
Hannah, Ricci; D'Ostilio, Kevin ULg; Goetz, Stefan et al

Poster (2015, March)

Detailed reference viewed: 11 (2 ULg)
See detailImaging air filled porosity in sea ice cover: Implication for sea ice permeability and gas exchange at the ice-atmosphere interface
Crabeck, O,; Galley, R,; Else, B, et al

Poster (2015, March)

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See detailHow snow affects air-sea ice CO2 fluxes ?
Delille, Bruno ULg; Kotovitch, Marie ULg; Van Der Linden, Fanny ULg et al

Poster (2015, March)

Detailed reference viewed: 14 (2 ULg)
See detailBlue sky and green bugs – How physical parameters and algal speciation influence DMSP and DMS profiles in Antarctic winter sea ice
Uhlig, C.; Rintala, J.-M.; Tison, J.-L. et al

Poster (2015, March)

Detailed reference viewed: 10 (0 ULg)
See detailThe role of sea ice in the carbon cycle of Polar Seas: 1D to 3D modelling
Moreau, S.; Vancoppenolle, M.; Delille, Bruno ULg et al

Poster (2015, March)

Detailed reference viewed: 18 (3 ULg)
See detailMeasurements of air-ice CO2 fluxes over artificial sea ice emphasize the role of bubbles in gas transport
Kotovitch, Marie ULg; Moreau, Sébastion; Zhou, Jiayun et al

Poster (2015, March)

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See detailTeaching and Training TA
Delvenne, Pierre ULg; Rosskamp, Benedikt ULg; Bütschi, Danielle et al

Poster (2015, February 26)

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See detailAdoption by gay and lesbian parents: a cross-nationale study
Messina, Roberta ULg; D'Amore, Salvatore ULg; Green, Robert-J

Poster (2015, February 13)

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See detailRisk of emergence of a hyperpathogenic bovine leukemia virus by mutation of a single envelope N-linked glycosylation site
De Brogniez, Alix ULg; Bouzar, Amel-Baya; Jacques, Jean-Rock ULg et al

Poster (2015, February 11)

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is ... [more ▼]

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is exemplified by the bovine leukemia virus (BLV) system in which lymphoproliferative disorders develop in ruminants after latency periods of several years. Infection of sheep and cattle with BLV is a model system for the related human T-lymphotropic virus type 1 (HTLV-1) responsible for Adult T-cell Leukemia (ATL). - Aims : The goal of this work is to investigate the role of N-glycans of the viral envelope protein during viral replication and pathogenesis. - Methods and results : Using glycosylation inhibitors and lectins, we showed that N-glycosylation is involved in viral infection (i.e. cell-to-cell fusion). Using reverse genetics of an infectious molecular provirus, we next demonstrated that a particular N-linked envelope glycosylation site (N230) limits viral replication and pathogenicity in vitro and in vivo. We have thus generated a viral mutant that is more pathogenic than the wild type strain. - Conclusions : To our knowledge, this is the first time that a hyperpathogenic BLV has been identified. This unexpected observation has important consequences in terms of disease control and managing. Indeed, during evolution, pathogens and their hosts should achieve an equilibrium allowing the coexistence of the two species. Occurrence of this particular mutation may thus represent a potential threat associated with emergence of hyperpathogenic BLV strains and possibly of new variants of the related HTLV-1. [less ▲]

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See detailCheckpoints modulation by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein : towards new therapeutic approaches
Carpentier, Alexandre ULg; Barez, Pierre-Yves ULg; Boxus, Mathieu et al

Poster (2015, February 11)

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. We are interested in the mechanisms of cell transformation by HTLV-1 and more particularly in the interplay between the viral Tax oncoprotein and the DNA damage response (DDR). We demonstrate that transient expression of Tax results in DNA damage, cell cycle arrest and activation of the ATM-Chk2-p53 axis of the DDR. In fibroblasts, cell cycle arrest occurs at the G1 and G2 phases depending on the p53 background. Despite Tax expression hampers cell cycle progression, neither pro-apoptotic nor pro-senescent effects are observed. In contrast, HTLV-1 infected lymphocytes proliferate continuously and appear to be adapted to the checkpoints. This mechanism allows infected lymphocytes to proliferate despite the presence of genomic lesions. Those cells might thus rely on effective DNA repair mechanisms. Indeed, we show that Tax expressing cells activate the error free repair mechanism homologous recombination (HR). Inhibition of ATM, involved in DDR and DNA repair by HR, impedes Tax-mediated cellular transformation. Depending on these observations, we propose a novel therapeutic approach based on the principle of synthetic lethality. [less ▲]

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, February 11)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailThe phosphorylation of RelA on Ser547 does not modulate NF-kB activation after TNFa treatment like after a genotoxic stress
Trussart, Charlotte ULg; Orban, Tanguy ULg; Di Valentin, Emmanuel ULg et al

Poster (2015, February 06)

NF-kB controls the expression of hundred of genes involved in inflammatory and innate responses, proliferation, survival, cancer initiation and progression. Numerous post-translational modifications of ... [more ▼]

NF-kB controls the expression of hundred of genes involved in inflammatory and innate responses, proliferation, survival, cancer initiation and progression. Numerous post-translational modifications of p65 modulating NF-kB transcriptional activity are known. We identified Ser547 as a new site of p65 phosphorylation targeted by ATM kinase, which coordinates the “DNA Damage Response” pathway in the event of DNA double-strand breaks. We demonstrated that the phosphorylation of Ser547 regulates the transcription of a sub-set of NF-κB dependent genes after genotoxic stress by modifying HDAC1 recruitment(1). Presently, we are investigating the role of this specific phosphorylation in an inflammatory context. We observe that the mutations of p65 (S547A or S547D) also affect the transcriptional potential of the NF-κB in a promoter specific manner after an exposition to TNFα and H2O2. The study of the molecular mechanism of this regulation after TNFα and H2O2 exposition are both in progress. [less ▲]

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See detailDaily intake and bacteriological quality of meat consumed in the households of Kigali city (Rwanda)
Niyonzima, Eugène; Ongol, Martin Patrick; Kimonyo, Anasthase et al

Poster (2015, February 05)

Meat is worldwidely known to be a nutrient rich food. It provides valuable amounts of proteins,vitamins such as retinol and vitamin B12 and minerals namely iron, selenium and zinc with an increased ... [more ▼]

Meat is worldwidely known to be a nutrient rich food. It provides valuable amounts of proteins,vitamins such as retinol and vitamin B12 and minerals namely iron, selenium and zinc with an increased bioavailability than found in other dietary sources [1]. Along the production chain, meat can get contaminated by a wide range of spoilage and/or pathogenic microorganisms from the farm, slaughtering environment and distribution. The actual number of foodborne infections attributable to meat is difficult to assess accurately, principally because only a small proportion of illness cases is officially reported. However, by using outbreak data published internationally, Greig and Ravel [2] reported that 12.7 % of reported foodborne outbreaks were attributable to beef while 10.5 and 4.6 % were associated with chicken and pork, respectively. According to the same authors, Salmonella spp. and pathogenic E.coli, respectively, were identified as the causal agents in 32.9 and 34.6 % of foodborne outbreaks of bacterial origin attributable to beef. The objective of this study was to determine the meat consumption pattern in different socio-conomical categories of the population of Kigali city and to assess the bacteriological quality of the consumed meat. [less ▲]

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See detailSite-specific height-diameter allometry of Central African moist forests
Loubota Panzou, Grâce Jopaul ULg; Doucet, Jean-Louis ULg; Fayolle, Adeline ULg

Poster (2015, February 04)

In this study we aimed to identify the variation in height-diameter allometry between forest types and among species in Central African moist forests. We also examined the consequences on biomass ... [more ▼]

In this study we aimed to identify the variation in height-diameter allometry between forest types and among species in Central African moist forests. We also examined the consequences on biomass estimation. Two forest sites in southern Cameroon with contrasting levels of deciduousness. Height and diameter were measured for a total of 521 trees belonging to 12 timber species over a large range of diameter, 10-240 cm for the Ma’an site and 11-182 cm for the Mindourou site. Non-destructive height measurements were calibrated with destructive measurements for a total of 60 trees, 30 in each site. Commercial forest inventory data (n=7253 0.5ha plots) were gathered for the Ma’an (n=34 samples and 2101 plots) and Mindourou (n=117 samples and 5152 plots) sites. A total of ten allometric models (including asymptotic and non-asymptotic models) were fitted to the height-diameter data at species (n=12) and site (n=2) level. Biomass estimates were computed based on forest inventory data and general allometric models using both site-specific and published height-diameter equations. Given the strong correlation between the non-destructive and destructive height measurements we had confidence in using the non-destructive height measurements to establish site- and species-specific height-diameter allometric equations. The height measurements performed over a wide range of diameters, 10-240 cm, tended to support an asymptotic shape (and most often the Michaelis Menten model) for the height-diameter allometry either at species and site level. We identified a significant difference in height-diameter allometry between the two study sites. For a given diameter, trees tended to be taller in the more semi-deciduous Mindourou site than in the more evergreen Ma’an site, with a maximum height of 39.5 and 46.5 m, respectively. The two sites significantly differed in stand structure and biomass. This difference is due to the variation in height-diameter allometry. Height-diameter allometry strongly varies between sites and site-specific height-diameter allometric equations should be developed to further improve the estimation of biomass and carbon stock contained in tropical forests. [less ▲]

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See detailSingle-Molecule Force Spectroscopy on Synthetic Helical Nanoarchitectures
Devaux, Floriane ULg; Duwez, Anne-Sophie ULg; Huc, Ivan et al

Poster (2015, February)

Foldamers are artificial folded molecular architectures inspired by the structures and functions of natural biopolymers. Folding is the process selected by nature to control the conformation of its ... [more ▼]

Foldamers are artificial folded molecular architectures inspired by the structures and functions of natural biopolymers. Folding is the process selected by nature to control the conformation of its molecular machinery to carry out chemical functions and mechanical tasks, such as en-zyme catalysis, duplication in nucleic acids, force generation,... During the last decade of research on foldamers [1], synthetic oligomers able to adopt well-defined and predictable folded conformations, such as helices, have been proposed. Recent progress has shown that stepwise chemical synthesis and molecular design based on aromatic oligoamide backbones enable to produce large helically folded molecular architectures. The shape and stiffness of the backbone, local conformational preferences, specific interactions between distant monomers in sequences, as well as the action of external parameters such as the solvent or the presence of ions, can be combine to induce folding tendency. A remarkable aspect of these architectures is that they can give rise to folded patterns that have no in natural counterparts biopolymer structures. For instance, helices whose diameter varies along the se-quence, helices possessing a handedness inversion centre, herringbone helices have been reported. The objective of the project is to synthesize various helical nanorchitectures based on an oli-goamide aromatic backbone and to obtain a detailed picture of their dynamical conformation in solution, as well as, their mechanochemical properties, by AFM-based single molecule force spectroscopy. It is worth mentioning that an important sub-objective of this project is to probe intramolecular interactions in small synthetic molecules with the AFM. Indeed, whereas single-molecule force spectroscopy on macromolecules (proteins and synthetic polymers) is widely exploited[2], implementing single-molecule force spectroscopy on small molecules, such as the foldamers proposed here, remains a major challenge[3]. [1] For a review, see G. Guichard and I. Huc, Chem. Commun. 2011, 47, 5933–5941. [2]E. M. Puchner, H. E.Gaub, Curr. Opin. Struct. Biol. 2009, 19, 605–614. [3]P. Lussis, A.-S. Duwez, Nature Nanotech. 2011, 6, 553-557 [less ▲]

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See detailScanning the genome for QTL affecting the recombination process in the male and female cattle germline
Kadri, Naveen Kumar ULg; Harland, Chad ULg; Coppieters, Wouter ULg et al

Poster (2015, February)

We herein study genetic recombination in three dairy cattle population from France, New-Zealand and The Netherlands. We apply a new phasing algorithm extracting familial information suited for large half ... [more ▼]

We herein study genetic recombination in three dairy cattle population from France, New-Zealand and The Netherlands. We apply a new phasing algorithm extracting familial information suited for large half-sib families to reconstruct haplotypes and detect cross-overs. The software is robust to genotyping errors and map errors (genome builts still contain errors for non-model organisms). We identify more than 2,000,000 cross-over events in sperm cells transmitted by 2942 sires to 94,049 offspring, and more than 500,000 cross-over events in oocytes transmitted by 10,943 cows to 23,850 offspring. The estimated number of cross-overs per gamete and its accuracy were influenced by the family structure (number of offsprings, parents and grand-parents genotyped). The average number of cross-overs in males (24.0) was larger than in females (21.8), even after correction for family structure. In males, recombination rates were higher closer to telomeres whereas in females, recombination rates dropped at both centromeres and telomeres (probably as a result of lower informativity). The heritability of the global recombination rate was close to 0.20 in males and to 0.10 in females and the genetic correlation was ~0.70, indicating that common genes are influencing both traits. Genome-wide association studies clearly confirmed QTL located close to REC8 and RNF212 in males. The QTL associated to REC8 was also detected in females and there was a positive correlation between QTL effects in males and females. The QTL associated to REC8 accounted for ~10% of the genetic variance in both males and females. [less ▲]

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See detailMultiplicity editing in long-range heteronuclear correlation NMR experiments: Application to natural products
Sauri, Josep; Sistare, E.; Frederich, Michel ULg et al

Poster (2015, February)

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See detailRole of the progesterone receptor in the development of sexual behavior in female mice
Desroziers, Elodie ULg; Brock, Olivier; Baum, M.J. et al

Poster (2015, February)

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See detailLordose et/ou xyphose chez le porc : mise à l’épreuve de l’hypothèse héréditaire
Laitat, Martine ULg; Veillat, Emilie; Van Cauwenberge, Henry et al

Poster (2015, February)

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity ... [more ▼]

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity. Pathomorphologically, it may be comparable with Scheuermann’s kyphosis in man and so constitutes a spontaneous model for this humane kyphosis of the thoracic or thoracolumbar spine. In pigs, this condition may decrease the value of carcasses, making deboning efforts challenging. Three major and non‐exclusive hypotheses formulated to explain these back deformations are nutrition, intrauterine viral infection and inherited predisposition. The objective of the present study was to test the latter and, if possible, to identify a locus (some loci) associated with the affection. Forty‐eight pigs were included in this case‐control study. Based on a clinical examination and/or on a measure of the degree of spinal deformity, 25 pigs classified as affected were compared to 23 pigs considered as normal. A whole genome Single Nucleotide Polymorphism (SNP) analysis was performed using a 50,000 SNP array. DNA from forty‐seven samples (tail tissue or blood) was extracted while one sample was eliminated because of its poor quality. After applying quality controls, 40 pigs and 57,838 SNPs (on a total of 62,163) remained for further analysis. One SNP (ASGA0090747) located on Sus scrofa chromosome SSC8 crossed the genome‐wide significant threshold and is thus suspected of being associated with the lordosis and/or kyphosis phenotype. These results seem to confirm the hereditary hypothesis. Further investigations are however needed to confirm the suspected association. [less ▲]

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See detailPrévalence du virus du SDRP dans les élevages porcins
Czaplicki, Guy; Thilmant, Pierre; Van Der Stede, Yves et al

Poster (2015, February)

Porcine reproductive and respiratory syndrome (PRRS) causes major economic losses in pig farms. In different parts of the world, there is growing consensus that PRRS virus (PRRSv) eradication should be ... [more ▼]

Porcine reproductive and respiratory syndrome (PRRS) causes major economic losses in pig farms. In different parts of the world, there is growing consensus that PRRS virus (PRRSv) eradication should be performed on a regional basis, particularly in low density areas of pig production. The objective of the present study was to evaluate the apparent prevalence of PRRSv in Wallonia, a low density area of pig production (0.05 pig farms/km²). A phone survey was addressed to 173 of the 276 Walloon breeding pig owners. One hundred and thirty‐two farmers answered the questionnaire and serological tests were performed in 113 herds. If no vaccination was carried out on sows or on piglets, an Elisa test was performed on 10 breeding and/or 5 feeder pig serum samples collected between November 2011 and December 2012. An individual result was considered negative if the s/p ratio was <0.4; a herd was considered positive if an s/p ratio ≥0.4 was obtained by at least one of its pigs. Globally, at herd level and at animal level, the apparent prevalences were respectively 48% (IC95%: 39 – 57%) and 32 % (IC 95%: 29 – 35%). The present study has shown that half the Walloon breeding pig farms are serologically positive for PRRSv. Such prevalence in an area of low pig production supports the project for a regional PRRS control program. [less ▲]

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, January 31)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailMyoferlin Regulates Endosomal Trafficking and Tunes Cancer Cell Metabolism
Blomme, Arnaud; Costanza, Brunella; Thiry, Marc ULg et al

Poster (2015, January 31)

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See detailStudy of durability and the physico-mechanical properties of thermally modified wood
Ninane, Maxime; Jourez, Benoît ULg; Hebert, Jacques ULg

Poster (2015, January 30)

Heat-treated wood is a credible alternative for certain kinds of tropical timber. It is also an alternative for certain polluting wood preservatives. This study aims to determine the changes in physico ... [more ▼]

Heat-treated wood is a credible alternative for certain kinds of tropical timber. It is also an alternative for certain polluting wood preservatives. This study aims to determine the changes in physico-mechanical properties and durability of wood that is subjected to heat treatment. For this, we studied five species (oak, ash, natural beech and a variant known as steamed beech, poplar, Douglas-fir). For each species, we had fifteen reference samples and fifteen corresponding treated samples. The results show a decrease in the hygroscopicity and an increase in dimensional stability of heat-treated wood in relation to the degradation of hemicelluloses. The mechanical properties are influence variously, because heat-treated wood is stiffer but also more brittle. The modulus of elasticity increases slightly after treatment. Side hardness and compressive fracture of heat-treated wood may increase up to 30%. On the other hand, the modulus of rupture, the ability to slit and impact may decrease by 50%, 60% and 70%. The durability of heat-treated wood against wood-destroying fungi increases. However, within each species, the durability increase varies greatly. Additional tests conducted to understand this variability, suggests that the heat distribution is not homogeneous within the processing unit. Moreover, our work suggests that durability is related to the fixed carbon content and that this path would be interesting to explore in the future. [less ▲]

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See detailInfluence of cover crop management on sugar beet production
Hiel, Marie-Pierre ULg; Bodson, Bernard ULg

Poster (2015, January 30)

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See detailBiochemical methane potential of residues of three banana varieties from Cameroon : fresh and dry peduncles
Awedem wobiwo, Florent; Happi Emaga, Thomas; Fokou, Elie et al

Poster (2015, January 30)

In Cameroon , the ratio of energy cost to standard of living for both electric and fuel energy is higher than in USA and EU. The local energy needs could be met by valorizing local wastes in an ... [more ▼]

In Cameroon , the ratio of energy cost to standard of living for both electric and fuel energy is higher than in USA and EU. The local energy needs could be met by valorizing local wastes in an environmentally and economically sustainable way. Banana and plantain plants produce significant quantities of post-harvest biomass wastes such as bulbs, pseudo stems, leaf sheaths, petioles–midribs, leaf blades, peduncles, rachis and blossoms which can be converted to methane-rich biogas by anaerobic digestion (AD). AD is a natural process of degradation of organic materials. [less ▲]

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See detailUtility of removing cholesterol from an industrial by-product- Buttermilk powder
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, January 30)

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered ... [more ▼]

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered invaluable for many years. When cream is churned to butter the stable oil in water emulsion is destabilized and milk fat globule membrane (MFGM) is disrupted which is released in buttermilk. Thus, over the last two decades it has gained considerable attention due to its specific composition (proteins and polar lipids) attributed towards the MFGM. In account to this composition of buttermilk powder, it finds application in food industry as a natural stabilizing and emulsifying agent. It also finds application as a source of total solids and is commercially being added upto 10% in many food products. However, it has limitation in such applications due to the presence of cholesterol (approx. 80mg/100g). Cholesterol is also an integral part of MFGM and is released into buttermilk with MFGM. The most suitable techniques to remove cholesterol from buttermilk powder are using β-cyclodextrin and cholesterol oxidase enzyme. To valorize this abundantly produced industrial by-product of butter industry and increase its application in health food industry, as well as extend its application in pharmaceutical industry and preparation of liposomes, it is important to remove cholesterol from buttermilk. [less ▲]

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See detailLinking bees and flowers: mutualistic interaction networks to study ecosystem functioning
Uyttenbroeck, Roel ULg; Monty, Arnaud ULg

Poster (2015, January 30)

In recent years, much attention is going to pollinators, as they are important for the increasing food production while being threatened by agricultural intensification and other environmental drivers ... [more ▼]

In recent years, much attention is going to pollinators, as they are important for the increasing food production while being threatened by agricultural intensification and other environmental drivers. While honeybees (Apis mellifera L.) are generalist according to their flower preferences, wild pollinators’ flower preferences can go from generalist to very specialist. The same goes for plant species, which can be generalist or specialist in the pollinator species they need to get pollinated. The combination of a set of generalist and specialist plant species with a pollinator community consisting of generalists and specialists in a certain habitat, results in an interaction network between pollinators and the visited plant flowers. Studying this mutualistic interaction network is a time-consuming method, but can provide valuable data to calculate indicators of ecosystem functioning. One of the important conclusions that came already out of studies applying this method is that pollination systems are often more generalized than thought based on pollination syndromes. This poster will explore the method of studying mutualistic interaction networks with a focus on the sampling protocol, the indicators that can be derived from the data and their meaning. [less ▲]

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See detailInteractions of natural rhamnolpids produced by Pseudomonas aeruginosa with plant model membranes
Polo Lozano, Damien ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2015, January 30)

It is well known that chemical pesticides have harmful effects on human health and environment. In this context, the interest for alternative products such as biopesticides is increasing. Among them ... [more ▼]

It is well known that chemical pesticides have harmful effects on human health and environment. In this context, the interest for alternative products such as biopesticides is increasing. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various chain lengths linked through a glycosidic bond to one or two rhamnose moieties. The fatty acids are linked together through an ester bond. These molecules have shown several biological activities including plant defense stimulation. It has been suggested that this elicitor activity could be related to an interaction of rhamnolipids with the lipid bilayer of the plant plasma membrane (PPM) and lead to its destabilization, which can activate the plant defense signaling pathways. In this context, interactions of two rhamnolipids (Rha-C10-C10 and Rha-Rha-C10-C10) with biomimetic membranes of PPM such as Langmuir monolayers and multilayers were investigated using biophysical and in silico approaches. [less ▲]

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See detailWildflower strips for crop protection: What do we know ? What should we know ?
Hatt, Séverin ULg; Uyttenbroeck, Roel ULg; Bodson, Bernard ULg et al

Poster (2015, January 30)

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of ... [more ▼]

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of studies looking at the efficiency of WFS to control pests are not unanimous. Indeed, the enhancement of pest control seems to depend on (1) the ability of flowers to attract the natural enemies at the right moment and (2) the capacity of natural enemies to migrate into the adjacent crops to attack pests. Therefore, constituting appropriate flower mixes may be an essential lever to enhance the efficiency of pest control. In this context, using functional diversity is promising. To our knowledge, few studies have tested the impact of the functional diversity of a flower mix on insect abundance and diversity and the control of pests. Through this contribution, the insect diversity and abundance found to be associated with the different kinds of WFS and management applied will be discussed, as well as the further research needed. [less ▲]

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See detailElicitation of the defence mechanisms at plant case of Cucumis melo
Ydjedd, Siham; Kati, D.E.; Fauconnier, Marie-Laure ULg

Poster (2015, January 30)

The judicious use of elicitors could reduce the amount of pesticide necessary to protect crops and preservation of the environment. This strategy frequently called "stimulation of natural defenses ... [more ▼]

The judicious use of elicitors could reduce the amount of pesticide necessary to protect crops and preservation of the environment. This strategy frequently called "stimulation of natural defenses" arouses more and more interest in the phytosanitary field. [less ▲]

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See detailLangmuir film balance- A technique to study the interaction of β-cyclodextrin with cholesterol in milk fat globule membrane monolayers
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, January 30)

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane ... [more ▼]

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane (MFGM). It has tripartite structure consisting of a monolayer facing the inner triacylglycerol core and an external bilayer. This MFGM accounts for 2-6% of fat globule. The MFGM is composed of both polar lipids and proteins. Cholesterol is also present in MFGM and accounts for about 2% of MFGM. Langmuir film balance is a technique to study monolayers and is a useful tool to examine the effect of cholesterol removal on surface properties of MFGM. β-cyclodextrin has no surface activity of its own and is thus a useful tool to study the effect of cholesterol desorption on surface properties in membrane. The removal of monolayer cholesterol to the sub-phase is proportional to the area decrease of the monolayer at a constant surface pressure. Amount of cholesterol removed can also be calculated as a function of mean molecular area at a given pressure, time and temperature. Using the monolayer technique, it is possible to study the interactions between β-cyclodextrin and MFGM film as well as effect of cholesterol removal in altering the surface properties MFGM. [less ▲]

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See detailAzacytidine prevents experimental sclerodermic chronic graft-versus-host disease
Fransolet, Gilles ULg; Ehx, Grégory ULg; SOMJA, Joan ULg et al

Poster (2015, January 30)

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell ... [more ▼]

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell transplantation, 60% of the patients experience chronic GVHD while approximately 15% of them develop a sclerodermic form of chronic GVHD characterized by multiple organ fibrosis and loss of skin elasticity. Regulatory T cells (Tregs) play a pivotal role in the pathology of chronic GVHD by inhibiting alloreactive conventional T cells. Several studies have shown the hypomethylating agent Azacytidine (Aza) can demethylate the master transcription factor of Treg (Forkhead box protein 3 factor, FoxP3), thus promoting Treg differentiation of conventional T cells. This work investigates the impact of Aza in a classical murine model of sclerodermic chronic GVHD (B10.D2  BALB/cJ). Methods: Lethally irradiated BALB/cJ recipient mice were injected with 107 bone marrow cells + 7.107splenocytes from B10.D2 donor mice. Recipients were treated with subcutaneous injections of Aza at the dose of 0,5 or 2 mg/kg every two days from day 10 to 30 following transplantation. Mice GVHD was evaluated with five criteria (weight loss, activity, fibrosis, hair loss and mice posture; 0-1-2 points/criteria). Mice were sacrificed at a score of 8/10 (or > 20% weight loss). Results: Mice treated with Aza 0.5 mg/kg (n = 14) or 2 mg/kg (n = 17) had significant lower clinical scores compared to control ones (n = 15) after treatment. FACS analysis showed a higher proportion of Treg among CD4+ T cells in the blood of Aza 2 mg/kg mice than in control mice (P = 0.047), as well as a higher percentage of Tregs expressing the KI67 proliferative marker on the same day (P = 0.0005). Finally, analyses of the cellular blood components with Cell-dyn demonstrated that Aza 2 mg/kg treated mice were significantly lymphopenic as compared to control mice (P = 0.05). Conclusion : Aza prevented sclerodermic GVHD in this classical murine model of chronic GVHD. [less ▲]

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See detailInvestigating the Effects of Plant Root Exudates on PAHs Bioavailability to Soil Microorganisms in Contaminated Brownfields : Research Methodology.
Davin, Marie ULg; Lognay, Georges ULg; Fauconnier, Marie-Laure ULg et al

Poster (2015, January 30)

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs ... [more ▼]

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs) represent 17% of treated pollutants in Wallonia (Aldric et al., 2011). Current remediation techniques are rather expensive and technically demanding (Megharaj et al., 2011). Based on the observation that PAHs soil content decreases in the presence of plants (Cheema et al., 2010), the PhD aims at developing alternative PAHs remediation techniques in brownfields. It is articulated around three research axes. The first axis focusses on plant exudates and how they may improve PAHs bioavailability to soil microorganisms and enhance their degradation. This will be investigated by (i) characterizing several contaminated soils (physico-chemical parameters) and PAH content and factors of bioavailability, (ii) selecting a plant model and collecting root exudates, and (iii) evaluating the effects of exudates on PAHs bioavailability. The objective of the second axis is to evaluate the effects of plant exudates on PAHs degrading microorganisms by (i) comparing PAHs biodegradation in the presence/absence of exudates and (ii) assessing the potential toxic effects of exudate compounds on the microbial communities. The aim of the third axis is to study plant-pollutants interactions by (i) establishing the plant tolerance to several contamination levels and (ii) following PAHs bioavailability when facing real exudation rates, on the field. [less ▲]

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See detailNitrous oxide flux measurement with a closed chamber system : data treatment
Regaert, Donat ULg; Moureaux, Christine ULg; Heinesch, Bernard ULg et al

Poster (2015, January 30)

Nitrous oxide flux estimation from concentration measurements with a closed chamber system. Statistical data treatment to sort between relevant/irrelevant fluxes.

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See detail[18F]UCB-H as a new PET radiotracer for Synaptic vesicle protein 2A: A first clinical trial
Bahri, Mohamed Ali ULg; Stifkens, Mathieu; Bastin, Christine ULg et al

Poster (2015, January 27)

SV2A is widely distributed in the brain and has been demonstrated to be involved in vesicle trafficking. The critical role of SV2A in proper nervous system function is shown, e.g., by the fact that it is ... [more ▼]

SV2A is widely distributed in the brain and has been demonstrated to be involved in vesicle trafficking. The critical role of SV2A in proper nervous system function is shown, e.g., by the fact that it is a binding site and the primary mechanism of levetiracetam. Levetiracetam is an antiepileptic drug which has recently been suggested to reduce synaptic deficits in a mouse model for Alzheimer’s disease. We here aimed to investigate the cerebral distribution of [18F]UCB-H, which has a high affinity with the SV2A. Dynamic PET data of the head of 4 healthy volunteers were acquired over 100 minutes after injection of 170.4 ± 24.9 MBq of GMP produced [18F]UCB-H. The arterial input function (IF) was obtained by blood sampling. The IF was also derived from the dynamic data using the correlation coefficient method. Blood data revealed a consistent amount of [18F]UCB-H in whole blood and plasma indicating a very low degree of binding of the tracer to the red blood cells. The image-derived arterial IFs were showed to be very similar to the measured ones with a peak-ratio around 0.91 and an area-under-curve ratio about 0.98. The [18F]UCB-H PET data showed a high and rapid uptake in the grey matter structures, matching the known ubiquitous distribution of the SV2A in the brain. The kinetics of the tracer in the brain was characterized by an initial high uptake phase followed by rapid washout allowing the standard compartmental modeling (1-tissue, 2-tissue, and Logan Plot). The three models gave similar results with both the measured and image-derived IFs. The total distribution volume of the tracer in the brain was greater than 7 mL/cm3. Our results suggest that [18F]UCB-H is a good candidate as radiotracer for brain SV2A proteins and could be used for human studies. Image-derived IF showed to be useful for quantitative studies without the need to the arterial blood sampling. SV2A modifications may consequently be assessed in neurological pathologies such as Alzheimer’s disease. [less ▲]

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See detailLocalization of rDNA transcription sites within reptilian nucleoli
Bartholomé, Odile ULg; Franck, Claire; Thiry, Marc ULg

Poster (2015, January 27)

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See detailExploration of volumetric cerebral changes, with de micro-MRi, due to psychomotor exercise in mice
Moës, Florian ULg; Plenevaux, Alain ULg; Becker, Guillaume ULg et al

Poster (2015, January 27)

It's well know that exercise is good for health .In addition exercise has postive effects on cognition ,neurodegenerative disease and on mood. Some studies show that exercise has effect on brain so the ... [more ▼]

It's well know that exercise is good for health .In addition exercise has postive effects on cognition ,neurodegenerative disease and on mood. Some studies show that exercise has effect on brain so the aim of this study is to see if there are volumetric changes due to exercise or not. [less ▲]

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See detailDéveloppement de nouveaux marqueurs neuroradiologiques de la maladie de Parkinson par reconnaissance de motifs
Himri, Khadidja ULg; Depierreux, Frédérique ULg; GARRAUX, Gaëtan ULg

Poster (2015, January 27)

Background and objectives: Automatic classification of Parkinson’s disease (PD) versus healthy controls (HC) based on structural MRI has so far focused on unimodal approaches. However, this method is ... [more ▼]

Background and objectives: Automatic classification of Parkinson’s disease (PD) versus healthy controls (HC) based on structural MRI has so far focused on unimodal approaches. However, this method is subject to a poor temporal and spatial resolution leading to low classification accuracy. To overcome this limitation we propose to integrate different modalities by generating a single decision function based on a multi-kernel method, exploiting the complementary information it offers. We predict that the integration of multiple modalities produces greater classification enhancement. Materials and methods: 3Tesla MRI was acquired in 42 patients with PD and 42 age and gender matched healthy controls. We relied on Unified Parkinson’s Disease Rating Scale (UPDRS) for evaluating the clinical status. We used structural and quantitative maps of T1, T2*, proton density (PD), magnetization transfer (MT), Multi-parameter (MT magnetization transfer, proton density (A), Iron Deposit (R2 *), mixing water content, iron, and the fraction of macromolecules tissues (R1) at 1 × 1 × 1 mm3 resolution. We identified cortical and subcortical brain regions (cortex, putamen, globus pallidus, substantia nigra), and cortical grey matter. We applied existing classification algorithms in the field of neuroscience using a classification algorithm based on Support Vector Machines (SVMs) [1], executed using the Pattern Recognition for Neuroimaging Toolbox (PRoNTo) [2]. The processes of classification was the following, data were mean centered and leave one subject out cross-validation was performed, making the test set independent from the training set. Analyses were restricted to voxels where all subjects had non-zero values. Statistical significance of the classifications was tested using permutation testing (1000 permutations) with random assignment of group class to the input image. Subsequently, we combined different modalities (MT, A, R1, R2) and identified the combination giving the highest sensitivity and sensibility in PD classification. As classifier we used support vector machines that are inspired by statistical learning theory Vladimir Vapnik and Multiple Kernel Learning approach, introduced by Lanckriet [3],[4]. Our approach can be seen as an analogue of MKL with SVMs. Conclusion & Future work: Identification of brain areas with affected intensity in the Parkinson’s group compared to Healthy Controls in single modalities using pronto is helpful. However, the subsequent multi-kernel approach utilizes unimodal information in a combined fashion so that emergent information is obtained, transcending effectiveness unimodal approaches. In conclusion, our findings suggest that combining different imaging modalities and different regions of interest increase classification accuracy significantly. These results are promising for objective diagnosis in medical practice. [less ▲]

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See detailMyoferlin regulates endosomal trafficking and tunes cancer cell metabolism
Blomme, Arnaud; Costanza, Brunella; Thiry, Marc ULg et al

Poster (2015, January 27)

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See detailMyoferlin: an indispensable component in VEGR-A secretion by pancreatic cancer cell
Fahmy, Karim; Gonzalez, Arnaud; Arafad, Mohamed et al

Poster (2015, January 27)

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See detailEffects of α-synuclein levels on cerebral synaptic function: Validation of a novel PET radioligand for the early diagnosis of Parkinson’s disease
Tarragon Cros, Ernesto ULg; Ferrara, André ULg; Tirelli, Ezio ULg et al

Poster (2015, January 27)

Background In Parkinson’s disease, converging evidence supports a pathogenic role for excessive α–synuclein accumulation in synaptic terminals that may propagate back to the soma of vulnerable nerve cells ... [more ▼]

Background In Parkinson’s disease, converging evidence supports a pathogenic role for excessive α–synuclein accumulation in synaptic terminals that may propagate back to the soma of vulnerable nerve cells such as neurons in the substantia nigra pars compacta. The resulting loss of dopaminergic terminals in the striatum can be demonstrated in vivo using 18F-Dopa-PET (positron emission tomography). However, there’s currently no validated biomarker of the progressive synaptic dysfunction in other vulnerable areas such as the cerebral cortex. Goal In this longitudinal study, we will test the hypothesis that the loss of synaptic terminals in a mouse model of excessive α–synuclein accumulation can be demonstrated in vivo before the occurrence of behavioural disturbances using 18F-UCB-H, a new PET biomarker developed at CRC. We will also test if this new imaging modality is sensitive enough to study the effect of a disease modifying therapy such as chronic physical exercise. Methods We will use microPET for the in vivo quantification of 18F-UCB-H brain uptake in 16 wild type animals and 16 transgenic (Tg) mice overexpressing human α–syn under the mThy1 promotor every 2 months. Data will be validated against post-mortem analyses after the last PET study. Predictions We predict decreased tracer uptake over time in the basal ganglia and cerebral cortex in Tg mice as compared with WT animals. Also, we predict a relationship between 18F-UCB-H uptake levels in basal ganglia and cerebral cortex and progressive alterations in both motor and cognitive functions, respectively. Further, we also expect that chronic exercise will slow down both motor and cognitive disturbances, as well as the rate of 18F-UCB-H brain uptake decreases. Conclusion If 18F-UCB-H PET proves to be a valid biomarker for the early detection of α–synuclein accumulation in the pre-clinical model of PD, the methods will tested on human clinical populations. [less ▲]

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See detailAccumulation of methylglyoxal, a glycolysis by-product modulates YAP1 transcription co-factor localization and activity in human breast cancer cells through HSP90 modification
Nokin, Marie-Julie; Durieux, Florence; Peixoto, Paul et al

Poster (2015, January 27)

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, January 27)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailBacterial DNA mimetics activate platelets and promote thrombosis via CLEC-2
Delierneux, Céline; Hego, Alexandre; Lecut, Christelle et al

Poster (2015, January 27)

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See detailHuman cortical excitability depends on time awake and circadian phase
Gaggioni, Giulia ULg; Ly, Julien; Chellappa, Sarah Laxhmi ULg et al

Poster (2015, January 27)

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See detailLa personnalité Borderline à travers le test de Rorschach
Rommes, Jennifer; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailÉtude du Typus mélancholicus au sein d’une population de dépressifs unipoalires
ramackers, Amélia; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailÉtude du récit de vie de l’adulte à haut potentiel
Mormont, Elodie; Gauthier, Jean-Marie ULg; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailThe Mediation Service, what interest for emergencies?
PIAZZA, Justine ULg; BRASSEUR, Edmond ULg; DOPPAGNE, Caroline ULg et al

Poster (2015, January 17)

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