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See detailLandslide characterization from space in the Rift flanks west of Lake Kivu (DRC)
Monsieurs, Elise ULg

Poster (2016, December 13)

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See detailSolar electrons observed in the magnetosphere
Shi, Quanqi; Wang, linghua; Zong, Qiugang et al

Poster (2016, December 12)

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See detailSmart Cities. Ingénierie des usages et co-conception : un nouveau cadre pour le développement durable des bâtiments et villes intelligents.
Schelings, Clémentine ULg; Elsen, Catherine ULg

Poster (2016, December 12)

Ce projet de recherche aborde le défi de l'opposition des utilisateurs finaux à certains des concepts clés de la ville intelligente (Smart City), et plus spécifiquement leur faible niveau d'engagement vis ... [more ▼]

Ce projet de recherche aborde le défi de l'opposition des utilisateurs finaux à certains des concepts clés de la ville intelligente (Smart City), et plus spécifiquement leur faible niveau d'engagement vis-à-vis du développement de leurs bâtiments intelligents. L'acceptation des utilisateurs finaux ayant une influence déterminante sur le taux d'échec / de réussite du projet Smart City en tant que tel, cette recherche se fonde sur l'expertise des concepteurs de la Smart City (architectes, ingénieurs architectes; ingénieurs en construction, ingénieurs en développement urbain et environnement, ...) et suggère qu'une partie de la solution relève de l'interaction que ces acteurs peuvent avoir avec les utilisateurs finaux au cours des phases préliminaires du processus de construction d'un projet. La recherche vise à développer des cadres théoriques dédiés (i) à l'ingénierie des usages et (ii) au processus de co-conception, de manière à outiller les concepteurs avec des modèles théoriques, des méthodes de travail et des outils adaptés pour (i) la capture et l'analyse des comportements des utilisateurs finaux et (ii) l'investissement actif des utilisateurs finaux au sein des processus de conception de bâtiments intelligents et de leurs modèles d'occupation intelligente. [less ▲]

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See detailRenverser la machine: de la technique comme outil d’exploitation à la technique comme moyen de régénération environnementale: l’approche paysagère comme préalable à la réhabilitation des sites industriels désaffectés dans la Province de Liège.
Baldin, Elisa ULg

Poster (2016, December 12)

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en ... [more ▼]

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en mettant à l’épreuve le principe de « recyclage » architectural et paysager. Le site Espérance Longdoz, à Seraing, représente, en tant qu’objet de mon travail de fin d’études, un cas spécifique mais indicatif d’un phénomène territoriale, qui concerne la Vallée de la Meuse. Le projet d’aménagement urbain, où le re-dessin de l’espace se fonde sur une re-interprétation des traces existantes, ouvre à une réflexion sur la complémentarité des éléments artificiels et des éléments naturels dans le projet de régénération urbaine. L’approche paysagère intégrée est proposée comme outil pour activer des nouveaux cycles de vie, selon une dynamique écologique fondée sur la continuité à trouver entre ressource et déchet: une alternative à l’abandon et à la disparition des lieux qui ont construit l’histoire sociale et l’identité du territoire. Lectures et observations des mutations morphologiques, complétées par des analyses comparatives entre expériences locales et européennes similaires concernant des réalisations, projets en cours et acteurs engagés, fondent une réflexion critique centrée sur le renversement du sens de la machine: autrefois celle qui a exploité et bouleversé les équilibres de la terre et aujourd’hui à l’opposé, celle qui se développe et transforme les milieux à travers temporalités et actions naturelles. La thèse explore les technologies soft, de phyto-dépollution ou autre procédés de transformation naturelle, à réinsérer dans le processus de mutation de nos milieux de vie comme un système de structures d’assainissement environnemental et comme un ensemble de stratégies de reconstitution d’un paysage productif urbain/périurbain, capables de réactiver des moyens soutenables aptes à « renverser la machine » d’exploitation industrielle. [less ▲]

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See detailFACILITER L'ADHÉRENCE AU TRAITEMENT IMMUNOSUPPRESSEUR CHEZ LES TRANSPLANTÉS DU REIN- TEST D'UN SYSTEME DE COACHING PAR SMARTPHONE
Saint-Remy, Annie ULg; Spiroux, Marie; WEEKERS, Laurent ULg et al

Poster (2016, December 08)

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM ... [more ▼]

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM. Krzesinski Introduction: Nonadherence to immunosuppressants is a determining cause of graft loss. The present study tested during 1 month, the usefulness and the effectiveness of a coaching system using smartphone application (the Transplant Smartcoach®) on adherence in kidney transplant recipients. Methodology: The sample included 51 patients (28m/23w) transplanted for 1 year at least, mean age 52±12 years, mean graft survival 3.7±1 years. Each patient had a smartphone configured with its detailed treatment. Using the smartphone application, patient had to notify daily the intake of each tablet into a 2-hour window. If no notification was performed 1 hour later than the scheduled time of intake, the patient was contacted by a nurse to remind him to take medications. Results: 90 % of patients were treated with Tacrolimus and 10 % with cyclosporine, associated with mycophenolic acid (41 %) or mycophenolate mofetil (49 %), 41 % had corticosteroids. Whatever was the immunosuppressant, a perfect adherence (medications taken at the scheduled time) was observed on average in 53 % of the morning monthly intakes. There was no difference in adherence rates between the morning intakes of Advagraf ® (once/day) and Prograft® (2 times/day), a decrease of perfect adherence was identified with the evening intake of Prograft® (53 % vs 44%; P=0.07) and the one of Myfortic® (P=0.03) with consequently an increased frequency of nurse’s recalls. Adherence was lower in younger patients and in those still working. The complexity of treatment (many drugs intake/day) and the respect for time intervals between drug intakes were the major barriers to adherence. When compared to the one measured before using the Smartcoach, the variability (coefficient of variation, %) of the Tacrolimus trough level decreased by 32.6 % (P=0.027) in the 3 to 6 months following the test. Conclusion: patients appreciated the ease and usefulness of the coaching system with smartphone application to help medication adherence. Coupled with therapeutic education of the patients, that tool deserves to be used notably in newly transplanted patients and when a worrying decrease in adherence is observed to help them in the management of a rigorous adherence which should contribute to graft survival. [less ▲]

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See detailXenogeneic graft-versus-host disease: Impact of Th17 cells.
Delens, Loïc ULg; SERVAIS, Sophie ULg; Vrancken, Louise et al

Poster (2016, December 08)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULg; Mendoza Gallego, Carlos ULg; Lismont, Marjorie ULg et al

Poster (2016, December 08)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 2 min while the same reaction in batch took 2h. [less ▲]

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See detailImplication of Importin-8 in mouse brain development
Nganou, Gerry ULg; Coumans, Bernard ULg; De Nijs, Laurence et al

Poster (2016, December 06)

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles ... [more ▼]

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles (like mitotic spindle formation, primary cilium transport). In situ hybridization performed in mouse brains slices shows that IPO8 is already expressed at E14. Moreover, inhibition of its action (IPO8) in embryonic mouse brain leads to impairment of neuroblast migration to upper cortical layers. IPO8 could then be at the origin of some pathologies with neuronal migration deficit. [less ▲]

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See detailOviposition deterring effect of Ocimum basilicum L. (Lamiaceae) on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)
Yarou, Boni Barthélémy ULg; Bawin, Thomas ULg; Boullis, Antoine ULg et al

Poster (2016, December 02)

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic ... [more ▼]

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and producers’ health, there is a need for alternatives. In this study, we assess the oviposition deterring effect of O. basilicum using dual-choice behavioral assays performed in flight tunnels. Two modalities were tested, i.e. (1) a tomato plant associated either with an O. basilicum plant versus a tomato plant associated with a plastic pot only filled with potting soil, and (2) a tomato plant with basil essential oil (EO) formulated in paraffin oil (PO) versus a tomato plant with PO only. A 1 ml cylindrical polyethylene plug loaded with 100 µl of solution (formulated EO or PO) was placed on each plant as a diffuser. Forty-eight hours after the release of unsexed adult individuals randomly sampled from the rearing in the central area of the tunnel, we found that plants and EOs reduced T. absoluta oviposition behavior on a nearby located tomato plant. GC-MS analyses showed that the major constituents include estragol (73.8%), linalool (8.6%), β-elemene (2.9%) in O. basilicum essential oils and E-α-bergamotene (38.9%), methyl eugenol (26.1 %), E-β-ocimene (17.7 % ) in O. basilicum VOCs collected with solid-phase micro-extraction method. These results suggest a valuable potential of O. basilicum and associated essential oils as component of an integrated management strategy against the tomato leafminer. [less ▲]

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See detailLe profil en fibres alimentaires de légume modifie leur fermentation intestinale et la production d’acides gras à chaine courte dans modèle in vitro humain
Kalala Bolokango, Gaetan ULg; Kambashi Mutiaka, Bienvenu ULg; Njeumen, Patrick et al

Poster (2016, December 01)

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des ... [more ▼]

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des changements dépendent du type de fibres.Les légumes sont des sources privilégiées de DF, certaines espèces et variétés fournissant davantage de DF solubles (SDF), d’autres étant plus riches en DF insolubles (IDF). Contrairement aux SDF, les IDF sont souvent considérées comme peu fermentescibles, ne contribuant que faiblement à une modulation de l’écophysiologie intestinale. En opposition à cette hypothèse, pour explorer si l’impact potentiel sur la santé intestinale des légumes varie suivant leur nature, des légumes présentant des teneurs et des profils en DF différents ont été comparés dans un modèle in vitro de fermentation colique. [less ▲]

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See detailGeneralized Pascal triangles and binomial coefficients of words
Stipulanti, Manon ULg

Poster (2016, December 01)

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word ... [more ▼]

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word. Similarly to the Sierpinski gasket that can be built as the limit set, for the Hausdorff distance, of a convergent sequence of normalized compact blocks extracted from Pascal triangle modulo 2, we describe and study the first properties of the subset of [0, 1] × [0, 1] associated with this extended Pascal triangle modulo a prime p. From the extended Pascal triangle obtained when p is equal to 2, we derive a sequence of which we study the regularity and the asymptotic behavior of the summatory function. Inspired from this regularity, we extend our results to another famous numeration systems, namely the Zeckendorff numeration system. [less ▲]

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See detailImportin-8 mutations could cause Jeavons Syndrome, CAE and JME by altering early neuroblast migration
Nganou, Gerry ULg; Tanaka, Miyabi; Coumans, Bernard ULg et al

Poster (2016, December)

Jeavons syndrome (JS) is an uncommon form of juvenile epilepsy. In some family affected by JS, the gene of importin-8 (IPO8) a member of karyopherin superfamily of proteins, has been found mutated ... [more ▼]

Jeavons syndrome (JS) is an uncommon form of juvenile epilepsy. In some family affected by JS, the gene of importin-8 (IPO8) a member of karyopherin superfamily of proteins, has been found mutated. Karyopherin are known to regulate nucleo-cytoplasmic transport of many proteins. IPO8, a member of the β-karyopherin sub-family, is reported to control the transport Ago-2, c-Jun and Smad-4 for example, three proteins important for brain development. Here, we show that IPO8 is well expressed in mouse brain at embryonic stage. Moreover inhibition of IPO8 mRNA by shRNA in-utéro-electroporation (IUE), impairs early neuroblast migration as weel as IUE overexpression of mutated form of human IPO8. So, abnormal brain development due to IPO8 mutations could be at the origin of Jeavons Syndrome, Chilhood abscence evolving (CAE) and/or juvenile myoclonic epilepsy (JME). [less ▲]

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See detailNEW INJECTABLE LIPOSOME AND DRUG-IN-CYCLODEXTRIN-IN LIPOSOME SYSTEMS ENCAPSULATING ESTETROL FOR THE TREATMENT OF ISCHEMIA DISEASES IN PREMATURE BABIES
Palazzo, Claudio ULg; Laloy, Julie; Delvigne, Anne-Sophie et al

Poster (2016, December)

Purpose: In 2010, almost 15 million of babies in the world are prematurely borned, 11.1 % of the total amount of alive children. Despite the better neonatology techniques, the number of preterm babies ... [more ▼]

Purpose: In 2010, almost 15 million of babies in the world are prematurely borned, 11.1 % of the total amount of alive children. Despite the better neonatology techniques, the number of preterm babies with motor, vision, hearing or mental deficiencies is still constant along the last twenty years. Moreover, no efficacy treatment is available to the present day. The estetrol (E4) has an important role in the brain development and protection. The aim of this study is to develop new injectable liposome and drug-in-cyclodextrin-in-liposome (DCL) formulations, encapsulating E4 in order to enhance its crossing through the blood-brain barrier (BBB). Methods: Cyclodextrins (CD) were used to increase E4 aqueous solubility. Liposome and DCL (E4-CD complex) formulations were prepared by thin-film hydration technique. The formulations were physicochemical characterized. LDH and MTS tests on endothelial, neuronal and BBB model cells were performed in vitro on the liposome formulation. Hemocompatibility of the formulations was evaluated on red blood cells, platelet aggregation and coagulation. BBB passage tests were performed using human BBB cell line (hCMEC/D3). Results: E4-CD complexes proportionally increased the solubility of the hormone. Liposomes and DCL encapsulating E4 were prepared. All the formulations had average particle size below 150 nm, polydispersity index below 0.10 and ζ potential around + 30 mV. The encapsulation efficacy for liposomes was between 3% and 10% while those of DCL are between 15% and 35%. Moreover, the formulations are capable to release 80 % (liposome) and 90 % (DCL) of encapsulated E4 after 3 h at 37°C. The effect of liposome and DCL formulations on cell viability and integrity was evaluated. The results showed no toxic effects on all the tested cell lines. Hemocompatibility tests showed no hemolysis, platelet aggregation or effects on coagulation, confirming the possibility of the formulations to be intravenously administrated. BBB passage tests highlighted the capability of the formulations to pass the BBB and reach the brain. Conclusions: New non-toxic, hemocompatible liposome and DCL formulations encapsulating E4 were prepared. The formulations are promising drug delivery system to target estrogens to the brain, due to their physiochemical characteristics. [less ▲]

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See detailDevelopment and comparison of liposomes and nanocapsules as injectable nanocarriers for poorly aqueous soluble drugs
Karim, Reatul ULg; Palazzo, Claudio ULg; Laloy, Julie et al

Poster (2016, December)

About 90% of drugs in development phase have poor aqueous solubility. Liposomes and nanocapsules are promising approaches that enable parenteral administration of these drugs with possibilities of site ... [more ▼]

About 90% of drugs in development phase have poor aqueous solubility. Liposomes and nanocapsules are promising approaches that enable parenteral administration of these drugs with possibilities of site specific delivery. The objective of the study was to develop different liposomes and lipid nanocapsules entrapping a hydrophobic model molecule (apigenin (AG)), and to characterize and compare them as potential injectable nanocarriers (NCs) for drugs with low aqueous solubility. [less ▲]

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See detailFirst Hubble Space Telescope Movies of Jupiter’s Ultraviolet Aurora During the NASA Juno Prime Mission
Grodent, Denis ULg; Gladstone, G. Randall; Clarke, John T. et al

Poster (2016, December)

The primary goal of this HST campaign is to complement Juno-UVS (Ultraviolet Spectrograph) observations. This complementarity is four-fold as HST observes Jupiter’s aurora when: 1) Juno-UVS is turned off ... [more ▼]

The primary goal of this HST campaign is to complement Juno-UVS (Ultraviolet Spectrograph) observations. This complementarity is four-fold as HST observes Jupiter’s aurora when: 1) Juno-UVS is turned off, that is about 98% of Juno’s 14-day orbit, and Juno’s in situ instruments are in operation. 2) Juno-UVS is operating, but observes the opposite hemisphere of Jupiter. 3) UVS is on in the same hemisphere, but too close to Jupiter to have a global, contextual, view of the aurora and/or UVS is affected by the noise induced by Jupiter’s radiation belts. 4) Juno is too far from Jupiter to get a detailed view of the aurora. In addition, HST will observe the auroral and airglow emissions of the Galilean moons Io, Ganymede and Europa, when UVS is measuring their auroral footprints in Jupiter’s ionosphere. During this campaign, HST is obtaining 45-min STIS time-tag images -movies- of both hemispheres of Jupiter and STIS/COS spectra of Jupiter's moons. These observations are taking place during 4 sequences of Juno's orbit (Figure: typical orbit in magnetic coordinates): 1) Perijove segment: a 6-hour sequence bracketing the time of Juno's closest approach of Jupiter. 2) Crossing segments: few hours periods during which Juno is crossing the magnetic equator of Jupiter and in situ instruments are observing the plasma sheet particles. 3) Perijove +/- 1 Jovian rotation (or more), to provide a context for the auroral activity before and after perijove. 4) Apojove segment: a 12-hour period bracketing the time when Juno is farthest from Jupiter and Juno-UVS is continuously monitoring the global auroral UV power of Jupiter. During Juno orbit PJ5, between 28 Nov. and 07 Dec. 2016, HST obtains 9 STIS movies: 3 movies of the northern aurora near perijove, 1 movie (north) one Jovian rotation before and 2 movies (south- north) one and two Jovian rotations after perijove, 2 movies (north) during two close CS crossings, and 1 movie near apojove. These movies will be commented during this presentation. [less ▲]

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Peer Reviewed
See detailMultianalytical Study of “Le Sorcier d’hiva Oa” by Gauguin
Defeyt, Catherine ULg; Remon, Regine; Strivay, David ULg

Poster (2016, December)

Although there is abundant literature on the worldwide famous French painter, Paul Gauguin, very little is known concerning his painting techniques and materials. The present research aims to throw a new ... [more ▼]

Although there is abundant literature on the worldwide famous French painter, Paul Gauguin, very little is known concerning his painting techniques and materials. The present research aims to throw a new light on Gauguin’s œuvre through the in depth study of Le Sorcier d’ Hiva Oa, painted by Gauguin during his stay in French Polynesia in 1902, by means of non-invasive imaging and analytical techniques. While the infrared reflectography examination of the painting revealed how the artist achieved his final composition, the results obtained by Raman spectroscopy and X ray fluorescence spectroscopy in punctual and mapping modes allowed identifying the different pigments he combined to obtain the bright color shades characterizing this artwork. [less ▲]

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See detailEtude de l’évolution de la pathologie arthrosique et des propriétés mécaniques du cartilage sur un modèle d’arthrose spontanée chez le cobaye Dunkin-Hartley
Legrand, Catherine ULg

Poster (2016, December)

Dans les modèles animaux, la gravité des lésions du cartilage est appréciée grâce à des scores histologiques évaluant la structure, le contenu en protéoglycane, l’intégrité de la tidemark, la cellularité ... [more ▼]

Dans les modèles animaux, la gravité des lésions du cartilage est appréciée grâce à des scores histologiques évaluant la structure, le contenu en protéoglycane, l’intégrité de la tidemark, la cellularité, ainsi que les ostéophytes. Parallèlement à ces analyses histologiques, nous avons étudié les propriétés mécaniques du cartilage à différents stades d’évolution de la maladie chez le cobaye Dunkin-Hartley. Nous avons également corrélé la sévérité des lésions histologiques avec les propriétés mécaniques du cartilage. [less ▲]

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See detailProperties of Jupiter’s auroral acceleration region inferred with HST-STIS spectral images
Ray, Licia C.; Arridge, Christopher S.; Gustin, Jacques et al

Poster (2016, December)

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. Precipitating auroral electrons are part of a current system which transports angular momentum from the planetary ... [more ▼]

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. Precipitating auroral electrons are part of a current system which transports angular momentum from the planetary atmosphere to sub-corotating magnetospheric plasma. The magnitude of the currents and hence precipitating energy flux, are sensitive to the characteristics of the high-latitude magnetosphere, in particular the location of the auroral acceleration region (AAR) and the density and temperature of the high-latitude electron population. We use HST STIS observations of Jupiter’s aurora (Gustin et al. [2016]) to infer the location of the AAR and the properties of the precipitating auroral electrons. To do this, we determine the energy of the precipitating electrons and incident energy flux for the two distinct regions within the main aurora and within flare regions. The resulting relationships between energy flux and electron precipitation energy for the main auroral emission are then compared to the theoretical relationship derived by Lundin & Sandahl [1978], in order to derive the location of the AAR and the temperatures and densities of the electrons at the top of the AAR prior to acceleration. We find that that each emission region is best reproduced using a multiple auroral acceleration regions with different properties, rather than a single auroral acceleration region with a varying potential drop strength. [less ▲]

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See detailComment mesurer correctement la PA chez les patients hémodialysés?
VANDERWECKENE, Pauline ULg; SAINT-REMY, Annie ULg; KRZESINSKI, Jean-Marie ULg

Poster (2016, December)

Objectifs : Le contrôle de la pression artérielle (PA) chez le patient hémodialysé (HD) est un enjeu majeur en raison des résultats controversés sur les risques cardiovasculaires qui y sont associés ... [more ▼]

Objectifs : Le contrôle de la pression artérielle (PA) chez le patient hémodialysé (HD) est un enjeu majeur en raison des résultats controversés sur les risques cardiovasculaires qui y sont associés. Cependant, la mesure de la PA dans cette population est difficile à appréhender au vu de sa grande variabilité. L’exactitude des mesures péridialytiques (enregistrées dans l’unité d’HD pendant la séance) est souvent remise en question. L’objectif de notre étude est d’établir la contribution et la concordance entre deux techniques de mesure ambulatoire pour détecter la PA non contrôlée chez ces patients HD. Il s’agit du monitoring ambulatoire interdialytique de 44h (MAPA) et de l’automesure au domicile (HBPM) pendant une durée variable de 3, 5 et 7 jours. Méthodologie : 43 patients hémodialysés chroniques (H=28, F = 15), avec une moyenne de 68,3±13 ans ont réalisé une MAPA de 44h (Spacelabs 90207), de la fin d’une séance d’HD au début de la suivante, immédiatement suivie par une automesure de 7 jours (Omron M6). Les mesures péridialytiques étaient la moyenne des PA pré-dialyse ou post-dialyse enregistrées sur 2 semaines (6 séances d’HD). L’hypertension était définie par une PA égale ou supérieure à 140/90 mmHg pour la PA pré-HD, 130/80 mmHg pour la PA post-HD, 130/80 mmHg pour la MAPA de 44 h et 135/85 mmHg pour l’HBPM. Résultats : Nous avons constaté de bonnes corrélations entre la MAPA et l’HBPM pour le diagnostic de l’hypertension chez le patient HD. Comparées aux PA péridialytiques, les 2 techniques ambulatoires ont permis d’identifier la même proportion d’hypertension masquée et d’hypertension de la blouse blanche (25%). La plus grande précision de diagnostic sur le status tensionnel est retrouvée pour les plus longues périodes d’enregistrement (44h pour la MAPA et 7 jours pour l’HBPM). Les plus courtes périodes étaient mieux tolérées par les patients mais moins précises pour détecter l’hypertension. L’automesure était plus appréciée que la MAPA de 44h. Conclusion : La MAPA et l’automesure ont permis d’identifier un quart de patients avec un phénotype particulier d’hypertension, qui n’aurait pas été classé correctement en se fiant uniquement aux mesures péridialytiques. L’automesure devrait être recommandée en première ligne chez le patient HD (au moins une semaine/mois). Cependant, la MAPA fournit des informations uniques comme la PA nocturne et pourrait être proposée, idéalement une fois par an, chez tous les patients. [less ▲]

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See detailIntramolecular continuous-flow strategy towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULg; Winter, Marc; Vizza, Alessandra et al

Poster (2016, December)

threo-Methylphenidate hydrochloride is the most widely prescribed drug to treat attention deficit hyperactivity disorder (ADHD). Herein we present the batch-to-flow translation of a carbene-based ... [more ▼]

threo-Methylphenidate hydrochloride is the most widely prescribed drug to treat attention deficit hyperactivity disorder (ADHD). Herein we present the batch-to-flow translation of a carbene-based intramolecular strategy for the diastereoselective preparation of this active pharmaceutical ingredient. The process relies on the thermal generation and decomposition of a hazardous diazospecies, its thermal intramolecular C-H insertion reaction yielding an intermediate β-lactam, and its subsequent acidic methanolysis, giving enriched threo-methylphenidate hydrochloride in 70% isolated yield. The automated microfluidic setup telescopes 2 chemical transformations, 1 intermediate in-line purification and sustains a daily productivity of 20.4 g/day of methylphenidate hydrochloride. The preparation of the β-lactam is implemented on mesoscale in a pilot-scale continuous-flow reactor, providing 4.25 kg of this key intermediate per day. [less ▲]

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See detailUn hydrogel de chitosan d’origine non-animale restaure la fonction rhéologique du fluide synovial arthrosique : étude ex vivo
Henrotin, Yves ULg; Rocasalbas, Guillem; Chausson, Mickaël et al

Poster (2016, December)

Detailed reference viewed: 31 (1 ULg)
See detailContinuous Monitoring of Jupiter's Aurora and Io Plasma Torus with the Hisaki Satellite during Joint Observing Campaign with Juno
Kimura, Tomoki; Yamazaki, Atsushi; Murakami, Go et al

Poster (2016, December)

The Hisaki satellite is the first space telescope that is dedicated to observations of our solar system bodies. Hisaki has been continuously monitoring the atmosphere and magnetosphere of the solar system ... [more ▼]

The Hisaki satellite is the first space telescope that is dedicated to observations of our solar system bodies. Hisaki has been continuously monitoring the atmosphere and magnetosphere of the solar system bodies with the extreme ultraviolet (EUV) spectroscope EXCEED since the launch in September 2013. Dynamics on timescales from 10s minutes to a few years were discovered in the atmosphere and magnetosphere by the continuous monitoring. Large joint observing campaigns for Jupiter by the Juno spacecraft in coordination with Hisaki, the Hubble Space Telescope (HST), and other facilities started in mid-2016. Before Jupiter Orbit Insertion, Juno made the in-situ solar wind measurements while Hisaki was simultaneously monitoring Jupiter's aurora and Io plasma torus. On Day of Year 142, Hisaki discovered that the EUV auroral emission power rapidly increased by a factor of ~3 but returned to a nominal level within one planetary rotation. The transient aurora was followed by brightening of the torus sulfur and oxygen ions that is proxy of the hot electron populations in the torus. At the same time, the relatively weak solar wind shock signatures were found in the JUNO/JEDI energetic particle data (Clark et al.). The transient variability is compared to the morphology observed by HST (Nichols et al.). Hisaki is going to monitor Jupiter in collaboration with the large observing program of HST (Grodent et al.) that will make ~150 exposures from this November. [less ▲]

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See detailMobility of 137Cs, 10Be and 210Pb in soil as a function of the soil texture, organic matter content and pH
de Tombeur, Félix ULg; Cornu, Sophie; Bourlès, Didier et al

Poster (2016, November 30)

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ... [more ▼]

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ecosystem services. In order to better constrain and quantify these processes, a kinetic quantification of the involved transfers based on vertical profiles of 210Pb (xs), 10Be and 137Cs has been developed for Luvisols in which soluble transfers could be neglected. The range of applicability of this type of approach was nevertheless not tested. In this work, we analyzed 210Pb (xs), 10Be and 137Cs in soils exhibiting gradients of organic matter, pH and < 2 μm fraction (Podzol, Andosol Ferralsol, Leptosol). Acidic soils and large organic matter contents were considered. The depth distribution of the studied isotopes was then interpreted as a function of the organic matter content, the soil pH and its < 2 μm fraction. This demonstrates that 10Be and 137Cs losses occur under soluble form in the Podzol, especially under forest, more acid, for the latter. Soluble 10Be transfers were also identified in the Andosol when they were considered as negligible in the Leptosol. For 210Pb (xs), soluble transfers are also probable for soils with pH less than 5.5 (Andosol and Ferralsol) and strong affinity for organic matter has been demonstrated. Thus, under acidic conditions and low < 2 μm fraction, soluble transfer cannot neglected anymore. [less ▲]

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See detailThe influence of specific training on the plyometric profile
Jidovtseff, Boris ULg; Harris, Nigel; Carpent, Nicolas et al

Poster (2016, November 30)

Sprinting, jumping and change of direction involve the stretch-shorten cycle (SSC) and therefore plyometric training is used to improve these factors. It has been demonstrated that the jumping strategy ... [more ▼]

Sprinting, jumping and change of direction involve the stretch-shorten cycle (SSC) and therefore plyometric training is used to improve these factors. It has been demonstrated that the jumping strategy utilized can influence the biomechanical profile of the exercise and subsequent adaptation. For example, in some cases, it is important to jump as high as possible while in other cases it is important to reduce the ground contact time. Our hypothesis is that the choice of plyometric exercise has to be matched to the desired biomechanical adaptation. Hence we investigated the influence of 8 weeks of different programs on the plyometric profile of recreational athletes. In each of five groups, very specific exercises were selected according to the training objective: ground contact time group (CT, n=9); vertical jump height group (JH, n=9); CT and JH combination group (CT-JH, n=11); JH + strength training group (JH-S, n=9); and control group (CO, n=8). The plyometric profile performed prior to and post training included measures of jump height, contact time, stiffness and reactivity at different bounding intensities. The results demonstrated that JH and JH-S programs were more effective for improving jump height performance (+7-9%; p<0.005) compared to insignificant jump height changes in the CT and CO groups. CT-JH was the most effective on the reactivity index (+14%, p<0.005) although significant increases (+8%, p<0.05) were also observed in CT, JH and JH-S groups. CT was the only group to significantly decrease short contact time (-5%, p<0.05). ANOVA analysis revealed significant groups*session effect for jump height (p<0.01) and reactivity (p<0.005) but not for short contact time. The present study confirms that the principle of specificity is fundamental in plyometric training so the exercise selection should be developed cognizant of intended adaptations. Jump height and reactivity appear to be differentially affected by specific training practices. [less ▲]

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See detailForce-Velocity-Power Profiles in Elite Young Soccer Players
Miny, Kevin ULg; Jidovtseff, Boris ULg

Poster (2016, November 30)

The main purpose of this research was to assess the isoinertial Force-Velocity-Power (F-V-P) profile in young elite soccer players. Furthermore, this research aims to compare force, velocity and power ... [more ▼]

The main purpose of this research was to assess the isoinertial Force-Velocity-Power (F-V-P) profile in young elite soccer players. Furthermore, this research aims to compare force, velocity and power qualities among different age categories (under (U) 17, U19 & U21). The ultimate objective is to better understand the relationships between neuromuscular system and functional performance. [less ▲]

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See detailInvolvement of Ikbζ in glioblastomas and its potential implication in radioresistance
Dubois, Nadège ULg; Willems, Marie; Kroonen, Jérôme et al

Poster (2016, November 29)

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See detailViral glycoprotein gp150 promotes sexual transmission of Murid Herpesvirus-4
Zeippen, Caroline ULg; Javaux, Justine ULg; Xiao, Xue ULg et al

Poster (2016, November 28)

Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of ... [more ▼]

Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of immune response. Understanding these strategies is likely to be important to control infection. Interestingly, we recently observed that Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus infecting laboratory mice, could be sexually transmitted between mice. This model offers therefore the opportunity to understand the mechanisms underlying natural transmission. Some of these mechanisms could rely on the glycoprotein 150 (gp150), which could limit virus neutralization and promote the release of infectious particles from cells. In this study, we tested therefore the importance of gp150 in the context of MuHV-4 sexual transmission. Briefly, female mice were infected with WT or gp150- strains expressing luciferase. They were imaged with an in vivo imaging system to follow infection. When lytic replication was observed in the genital tract, infected females were mated with naïve males to compare the capacity of transmission of the two strains. Our results show that, while the gp150- strain has no deficit in reaching and replicating in the female genital tract, it displays a major deficit of sexual transmission in comparison with WT virions. Interestingly, this deficit appears to reflect a deficit of virions release from vaginal epithelial cells. Altogether, our results show that, while gp150 is not required for efficient dissemination and maintenance of MuHV-4 within its host, it is essential for efficient transmission, by promoting the releasing of infectious particles from the mucosal cells. [less ▲]

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See detailInterfacial and foaming properties of five commercial dairy products and two types of proteose-peptone fractions
Ripollés, D.; Parrón, J.A.; Petrut, R. et al

Poster (2016, November 28)

Functional properties of surfactants contained in dairy products, like emulsifying, foaming and wetting properties, result from their capacity to reduce the surface tension by adsorption and formation of ... [more ▼]

Functional properties of surfactants contained in dairy products, like emulsifying, foaming and wetting properties, result from their capacity to reduce the surface tension by adsorption and formation of cohesive films at the interface. A wide variety of techniques have been developed to determine surface tension, but those that give dynamic measurements, rather than equilibrium measurements of surface tension, are more suitable to study functional properties. Furthermore, the surface activity of surfactants depends on the mechanical properties of the films that are formed at the interface, which are strongly influenced by the intramolecular interactions established. The aim of this study was to investigate the kinetics of adsorption, mechanical properties of monolayer films and foaming properties of five different commercial dairy products (whey protein concentrate (WPC), buttermilk, butter serum and two skimmed milks) and two proteose-peptone fractions. [less ▲]

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See detailL'activation comportementale est-elle pertinente pour tous les adultes dépressifs ? Une analyse en clusters
Wagener, Aurélie ULg; Baeyens, Céline; Van der Linden, Martial ULg et al

Poster (2016, November 26)

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression ... [more ▼]

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression soulignent l’importance de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes dans le maintien de la dépression. Cette étude poursuit deux objectifs : (1) investiguer l’hétérogénéité de la depression via une analyse en clusters, (2) investiguer les valeurs prédictives de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes sur la dépression en fonction du cluster d’appartenance. METHODE : Des adultes tout venant et/ou consultant des centres de santé mentale ont été recrutés (âge entre 18 et 60 ans). Ils ont complété l’Inventaire de Dépression de Beck – Seconde Edition, l’Echelle d’Activation Comportementale pour la Dépression – Version courte, et l’Inventaire des événements plaisants. RESULTATS : Une solution en six clusters a été mise en évidence : “gros dormeurs/heavy sleepers”, “dépressifs cognitifs/cognitive depressives”, “dépressifs affectifs-somatiques/affective-somatic depressives”, “dépressifs légers/mild depressives”, “dépressifs dormant peu/sleepless depressives”, “dépressifs typiques/typical depressives”. Des relations significatives entre inactivité, fréquence d’activités plaisantes et dépression ont seulement été observées chez les “dépressifs cognitifs/cognitive depressives”, les “dépressifs légers/mild depressives” et les “dépressifs typiques/typical depressives”. DISCUSSION : Les résultats confirment l’hétérogénéité de la dépression puisque six clusters ont été mis en évidence. Par ailleurs, l’activation comportementale ne semble pertinente que pour trois de ces clusters dans lesquels les postulats du modèle comportemental de la dépression sont confirmés (“dépressifs cognitifs/cognitive depressives”, “dépressifs légers/mild depressives”, “dépressifs typiques/typical depressives”). [less ▲]

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See detailLa Perspective Temporelle Equilibrée: Validation d’un questionnaire et relation avec la régulation émotionnelle.
Barsics, Catherine ULg; Rebetez, Marie My Lien; Rochat, Lucien et al

Poster (2016, November 26)

La Perspective Temporelle Equilibrée fait référence à une disposition individuelle caractérisée par le fait de se projeter mentalement dans le futur et dans le passé de manière positive et fréquente ... [more ▼]

La Perspective Temporelle Equilibrée fait référence à une disposition individuelle caractérisée par le fait de se projeter mentalement dans le futur et dans le passé de manière positive et fréquente. L’objectif de notre étude était de valider la version française d’une échelle, initialement conçue en anglais, évaluant cette orientation temporelle : la “Balanced Time Perspective Scale” (Webster, 2011). Une étude en ligne a permis de recueillir les données de 622 participants francophones issus de la population générale. Les résultats indiquent que la version française réplique la structure en deux facteurs du questionnaire original et plaident en faveur d’une bonne validité de construit. De surcroît, les résultats montrent qu’une tendance importante à se projeter mentalement dans le futur et dans le passé de manière positive et fréquente est associée à la réévaluation cognitive, une stratégie de régulation émotionnelle. En somme, ce questionnaire permet d’évaluer la perspective temporelle, qui est ici envisagée en tant qu’importante ressource pour le self, et ce, en étroite relation avec les capacités de régulation émotionnelle. [less ▲]

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See detailAN IN-SILICO MODEL OF BMP-2 CARRIER DEVICE FOR BONE TISSUE ENGINEERING APPLICATION
Manhas, Varun ULg; Carlier, Aurelie; Geris, Liesbet ULg

Poster (2016, November 25)

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See detailEVALUATING THE SPECIFICITY OF [18F]UCB-H FOR THE ISOFORM SV2A, COMPARED WITH ISOFORMS SV2B AND SV2C
Serrano Navacerrada, Maria Elisa ULg; Aerts, Joël ULg; Bahri, Mohamed Ali ULg et al

Poster (2016, November 18)

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important in normal and pathological process, like the ... [more ▼]

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important in normal and pathological process, like the epilepsy (1, 2). [18F]UCB-H was developed like a tool to study the role of this isoform with neuroimaging techniques (3, 4). The objective of this study was to evaluate its specificity to this isoform comparing with the others, through a competition assay in rats with ex-vivo autoradiography and mPET imaging. Methods: Forty male Sprague-Dawley were used in ex-vivo autoradiography experiments (N=20) and in microPET imaging (N=20). Animals were pre-treated 30 minutes before the injection of [18F]UCB-H with a dose IP either of vehicle, Keppra (SV2A ligand), UCB068 (SV2B ligand) or UCB054 (SV2C ligand). Ex-vivo autoradiography was carried out 5 minutes after radiotracer injection while mPET images were acquiring with a dynamic scanner of 1 hour. Data were expressed in Standard Uptake Value and then, the area under the curve was calculated for the total process. Results: In ex-vivo autoradiography, ANOVA of two-ways showed statistical significant differences in brain uptake of [18F]UCB-H among the groups pretreated with Keppra or the ligand for SV2B and the control group. Regarding mPET data, statistical significant differences were found between the group injected with keppra and the rest of groups. Conclusion: Even if a considerable affinity between the ligands UCB068 and UCB054, and the receptor for the isoform SV2A exists, it is only detected during the first 5 minutes (ex-vivo technique), being certainly due to a nonspecific binding. This binding is not strong enough to show a direct competition with the radiotracer during a mPET acquisition. These results allow us to conclude that [18F]UCB-H is a suitable radiotracer for the imaging of the isoform SV2A in vivo, allowing us the clinical study about the molecular base of a disease with a high population impact, like the epilepsy. [less ▲]

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See detailEducation, Complexity of Work, and Incident Cognitive Impairment in Puerto Rican Older Adults
Andel, Ross; Luisa, Ana; Small, Brent et al

Poster (2016, November 18)

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See detailCrosstalk between VEGF and BMP9 pathway. Implications in HHT disease
Pollenus, Thomas ULg

Poster (2016, November 18)

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See detailLimited impact of imatinib in a murine model of sclerodermic chronic graft-versus-host disease
Belle, Ludovic; Fransolet, Gilles ULg; SOMJA, Joan ULg et al

Poster (2016, November 17)

Background: Graft-versus-host disease (GVHD) remains one of the major complications following haematopoietic stem cell transplantation (HSCT). Approximately 15% of the patients with chronic GVHD develop ... [more ▼]

Background: Graft-versus-host disease (GVHD) remains one of the major complications following haematopoietic stem cell transplantation (HSCT). Approximately 15% of the patients with chronic GVHD develop the sclerodermatous form of the disease characterized by multiple organ fibrosis and loss of skin elasticity. Several studies have shown the potential benefits of imatinib, a tyrosine kinase inhibitor, as a treatment of fibrosis in cGVHD due to its ability to inhibit simultaneously PDGF-R and c-Abl pathways which are both involved in fibrosis mechanisms. This work investigates the possible benefits of imatinib on fibrosis in a murine model of sclerodermatous chronic GVHD (sclcGVHD). Methods: Lethally irradiated Balb/cJ mice (7 Gy TBI) were injected i.v. with 10.106 bone marrow cells and 70.106 splenocytes from B10.D2 donnor mice. Mice were then treated with sterile water or imatinib (150 mg/kg/day) by oral gavage from day +7 to day +52 following transplantation. GVHD severity was assessed three times/week with a scoring system encompassing 5 criteria (mice posture, weight loss, activity, hair loss, skin integrity ; 0-1-2 point(s)/criteria). Results: Our results show that imatinib failed to prevent/improve GVHD with a similar evolution of the GVHD severity with no differences between groups. Histological analyses indicate a significant reduction of the phosphorylation level of the PDGR receptor (p = 0,033) and a trend to a decreased level of phosphorylated c-Abl (p = 0,1854). In vivo cell proliferation assay with CFSE were also performed and showed a reduced proliferation of T cells and subsets (CD4, CD8 and Tregs) in spleen, lymph nodes, bone marrow and blood after imatinib treatment. Finally, FACS analyses performed on days +21 and +35 after transplantation did not show any differences in the absolute T-cell counts. Conclusion: Although we have observed a decreased phosphorylation level of PDGR receptor and less proliferation of T cells and subsets in vivo, imatinib failed to alleviate scl-cGVHD in a murine model of severe scl-cGVHD. [less ▲]

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See detailHot-Melt Extrusion as a Continuous Manufacturing Process to Form Ternary Cyclodextrin Inclusion Complexes
Thiry, Justine ULg; Krier, Fabrice; Ratwatte, Shenelka et al

Poster (2016, November 16)

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See detailPolyphenol content and antioxidant capacity in various commercial juices
Matute Matute, Alexis Fernando ULg; Tabart, Jessica; CHERAMY-BIEN, Jean-Paul ULg et al

Poster (2016, November 16)

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free ... [more ▼]

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free radicals promoting positive effects on human health due to their antioxidant capacity. In this study we evaluated the content of polyphenols from several commercial biological (n = 6) and conventional juices (n = 6 ) (orange, blackcurrant, apple, grapes, tomato, carrot, pomegranate, lemon, grapefruit juices) and we determined the antioxidant effect by inhibition of the superoxide anion production measured by chemiluminescence technology. Conventional juices (orange, apple, tomato, pomegranate, lemon, grapefruit juices) showed more total polyphenolics (+ 32%), more flavan-3-ols (+ 67%) and more flavonols (+ 42%) than the same biological juices. The presence of anthocyanins was reported in juices made with blackcurrant, pomegranate and grapes only, with values above 50 µg/mL, regardless of their nature. Surprisingly, chemiluminescence results showed an inhibition in superoxide anion production by 51% for biological juices vs 23% in conventional ones. The correlation between total polyphenol content and antioxidant capacity of all the juices was r = 0.83 (r2 = 0.7). The fact that there is less polyphenols and higher antioxidant activity in bio juices could be related to the concentration and the type of polyphenol present. [less ▲]

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See detailZinc, iron and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2016, November 16)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). [less ▲]

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See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, November 16)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells. We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood. : We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells. [less ▲]

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See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULg; Centonze, Prescilia ULg; Comblain, Fanny ULg et al

Poster (2016, November 16)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

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See detailDevelopment of a new method for arabidopsides extraction and purification
Genva, Manon ULg; Andersson, Mats X; Nasir, Mehmet Nail ULg et al

Poster (2016, November 16)

In the present work, a new method for arabidopsides extraction and purification was developed.

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See detailAnalysis of the interactions of fatty acyds hydroperoxides with biomimetic membranes by complementary biophysical approaches
Deboever, Estelle ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2016, November 16)

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants ... [more ▼]

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants which are able to induce the natural resistance of plants. Also, they have proved to be excellent candidates for biological control. In this context, the lipoxygenase pathway leads to the formation of fatty acid degradation products, called oxylipins, which appear to be crucial agents in plant defence mechanisms2,3. Moreover, with their broad spectrum of action and their possible inducibility, oxylipins appear to be promising candidates for their use as elicitors4. This work focuses on two hydroperoxy-derived oxylipins, the 13(S)-hydroperoxy-octadecadienoic acid (13-HPOD) and the 13(S)-hydroperoxy-octadecatrienoic acid (13-HPOT). The study of the interaction of such compounds with representative plant plasma membrane lipids is essential to understand plant resistance mechanisms. Several in silico and experimental techniques of biophysics showed that acyl-hydroperoxides have significant adsorption capacity and a strong affinity for model membranes. They may also penetrate biological membrane but no permeabilisation effect was observed in this work. Slight conformational differences seem to have a significant impact on their ability to interact with plant plasma membranes. Based on these results, further investigation of the interactions of fatty acids hydroperoxides, even more on the 9-forms, with plant plasma membranes and eventually in the presence of phytopathogenic species, would allow a better understanding of the innate immunity and, on the longer term, could lead to the development of new elicitors with biological mechanisms potentially independent of membrane protein receptors. [less ▲]

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See detailThe BCCM/ULC collection to conserve the biodiversity and study the secondary metabolites of Polar cyanobacteria
Lara, Yannick ULg; Durieu, Benoit ULg; Renard, Marine ULg et al

Poster (2016, November 16)

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in ... [more ▼]

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts. Their success in these harsh cold conditions can probably be explained by particular adaptations to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other environmental stresses. The BCCM/ULC public collection is funded by the Belgian Science Policy Office since 2011. It has obtained the ISO9001 certification for deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. This collection aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths,….) and make it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, pigments, and genomic make-up. It presently includes 226 cyanobacterial strains, of which 119 are of Antarctic origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). As shown by morphological identification, the strains belong to five orders (Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales). The 16S rRNA and ITS sequences of the strains are being characterized. The first 85 Antarctic strains already studied are distributed into 25 Operational Taxonomic Units (OTUs = groups of sequences with > 97,5% 16S rRNA similarity), and thus, represent a quite large diversity. Moreover, strains identified as members of the genera Leptolyngbya or Phormidium appear in several lineages. This supports the idea that there is a need to revise the taxonomy of these polyphyletic genera with a simple filamentous morphology. To better understand the functioning, metabolism and adaptative strategies of cyanobacteria in the extreme Antarctic environment, the genome sequencing of 11 strains has been started. Pair-read data from illumina MiSeq runs were obtained and submitted to a bioinformatic pipeline dedicated to the assembly of genomes and search of sequences involved in the biosynthesis of secondary metabolites. Gene cluster prediction analysis allowed to characterize 20 clusters of NRPS, PKS and hybrid NRPS-PKS from 2 to 66kb. Surprisingly, none of the characterized operons had previously been described in the literature. [less ▲]

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See detailA crucial role for cdk1 in postnatal hippocampal neurogenesis
Marlier, Quentin ULg

Poster (2016, November 15)

Cdk1 seems to be involved in the proliferation and differentiation of adult neural stem cells found in the adult dentate gyrus

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See detailLa promotion des énergies renouvelables : une réponse durable à la problématique énergétique au Tchad
Abdelhamid, Issa Hassane ULg

Poster (2016, November 14)

La promotion des énergies renouvelables est un thème d’actualité de par le monde. Chaque pays essaie d’apporter des solutions à son bilan énergétique compte tenu de ses paramètres dits de 3E (Énergie ... [more ▼]

La promotion des énergies renouvelables est un thème d’actualité de par le monde. Chaque pays essaie d’apporter des solutions à son bilan énergétique compte tenu de ses paramètres dits de 3E (Énergie-Économie-Environnement). Cette note de recherche privilégie une approche scientifique de l’énergie et du développement durable en l’appliquant au contexte tchadien.Le but assigné à ce poster est de mettre en exergue l'ampleur du patrimoine naturel tchadien en énergies renouvelables (biomasse, éolien, solaire, etc.) en le conciliant avec la nouvelle technologie afin de répondre aux besoins énergétiques des ménages n'ayant pas accès à l'énergie électrique au Tchad... [less ▲]

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See detailElektrodynamische Fragmentierung von Elektroschrott
Iseli, Cédric; Streicher-Porte, Martin; Martino, Rémi ULg et al

Poster (2016, November 11)

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden ... [more ▼]

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden kann. Verschweisste Kompaktgeräte können zum Beispiel so weit geöffnet werden, dass die ge-wünschte Komponente freigelegt und entfernt werden kann. Leiterplatten haben die Eigenschaft, dass bei kontinuierlich steigendem Energieeintrag zuerst die aufgebrachten Komponenten abgetrennt werden, bevor die Cu-Bahnen freigelegt werden. Bei noch höherem Energieeintrag wird die Leiterplatte schlussendlich zerkleinert und vollständig zer-stört. Durch diese Entfrachtung der Leiterplatte kann ein metallreiches Konzentrat hergestellt werden. [less ▲]

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See detailEndocrine disruptors and parasitism: which effects on the sex behaviour of the amphipod Gammarus pulex ?
Fivet, Adeline; Horion, Sibylle; Thomé, Jean-Pierre ULg et al

Poster (2016, November 10)

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See detailA Model of Endogenous Insulin Secretion During Exercise
Thomas, Felicity Louise ULg

Poster (2016, November 10)

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See detailBulk high-temperature superconducting hollow cylinders used for magnetic shielding: effect of an initial trapped field on the shielding performances.
Wera, Laurent ULg; Fagnard, Jean-François ULg; Hogan, Kevin ULg et al

Poster (2016, November 09)

Bulk High-Temperature Superconductors (HTS) can be used as efficient passive magnetic shields with performances exceeding those of conventional ferromagnetic materials. The most common geometry for a ... [more ▼]

Bulk High-Temperature Superconductors (HTS) can be used as efficient passive magnetic shields with performances exceeding those of conventional ferromagnetic materials. The most common geometry for a superconducting shield is a bulk hollow cylinder. In this work, we investigate the magnetic shielding properties when the tube is not in the virgin state. Such a situation happens in practice when the tube has been initially subjected to a first magnetic field. The induced superconducting currents that are flowing in the tube generate a trapped magnetic moment which may deteriorate the shielding of a second field. Here we study experimentally how an initial trapped moment affects the shielding of a magnetic field, when they are both perpendicular to each other. In particular, we focus on the effect of an axial trapped moment on the transverse shielding performances and on the effect of a transverse trapped field on the axial or the transverse shielding performances. Finally, we show how the pristine state of the tube can be restored when applying an external field of adequate amplitude. The consequence for practical applications is that it is possible to recover shielding properties similar to those obtained in the virgin state without heating up the shield above its critical temperature TC. [less ▲]

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See detailSevere deficiency of the somatotrope GHRH/GH/IGF-1 axis induces a dramatic susceptibility to Streptococcus pneumoniae infection.
Farhat, Khalil ULg; Bodart, Gwennaëlle ULg; Desmet, Christophe ULg et al

Poster (2016, November 07)

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation ... [more ▼]

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation (Alba & Salvatori, Endocrinology 2004;145:4134). In basal conditions, the immunological phenotype of Ghrh-/- mice is not markedly disturbed except for a decrease in B cells and an increase in generation of thymic (t) Treg cells (submitted for publication). These data prompted us to investigate immune responses of Ghrh-/- mice using vaccination and infection by S. pneumoniae as models since the response to both stimuli rely on the innate immune system and B cells. Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH differently impacts on B-1, marginal zone B-2 or innate B-1 B cells. Furthermore, after intranasal instillation of a non-lethal dose of serotype 1 S. pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility reflected by bacteremia 24h after infection and a survival limit of 72 h, compared to WT C57BL/6 mice that need only 24h to clear infection. The possible impact of GH deficiency on components of the innate immune system that play an important role in defense of the respiratory tract against pneumococcal infection is under current investigation. (*Equal first and last authors. KF is supported by a research grant from the Lebanese Government; GB is Research Assistant, CD is Research Associate, and VG is Research Director at the NFSR of Belgium). [less ▲]

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See detailPeople moved and will move again
Gemenne, François ULg; de Longueville, Florence; De Bruyckere, Luka ULg et al

Poster (2016, November 04)

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See detailDiagnostic value of serologic tests and seroprevalence of Borreliosis in horses living in southern Belgium
Cerri, Simona ULg; Meersschaert, C; Houben, Rosa et al

Poster (2016, November 04)

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See detailRecent evolution of the coastline in the Gulf of Guinea. Example of Togo and Benin (2000-2015)
Ozer, Pierre ULg; Hountondji, Yvon-Carmen; de Longueville, Florence

Poster (2016, November 04)

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See detailRisk management in Ivory Coast : Case Study of Population Evictions in Port-Bouët, Abidjan
Comoe, Raymond; Ozer, Pierre ULg

Poster (2016, November 04)

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See detail« Cap-Haïtien » or « how to ‘construct’ a flood risk in a decade »
Gracius, Gracia Joseph; Ozer, Pierre ULg

Poster (2016, November 04)

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See detailImpact of shoreline changes on population in Cotonou, Benin
de Longueville, Florence; Hountondji, Yvon-Carmen; Gemenne, François ULg et al

Poster (2016, November 04)

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See detailTerminologia Multilíngue de Clínica General / Medicina de Família e Comunidade
Jamoulle, Marc ULg; Treuherz, Arthur; Gomes, Luis Filipe et al

Poster (2016, November 02)

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently ... [more ▼]

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently addressed by GPs when they meet in congresses. A taxonomy has been build. A multilingual terminological record has been developed for each of the 182 items identified. This work is freely available online on www.hetop.eu/Q (free access after inscription) and is supported by the Rouen (France) biomedical informatics department of the University of Rouen (France). All the data are available in the Web Ontology language (OWL-2), an ontology-ready format fit for future use in the semantic web. The product, user guide and e-learning are available on http://3cgp.docpatient.net/ The Q-codes base is aimed at several uses: • Online repository of knowledge specific to GP/FM in several languages • Online PubMed linked bibliographic system easy to use for training in GP/FM • Resource for online e-learning • Resource for analysis of content of congresses in GP/FM in joint usage with ICPC-2 and indexation of gray literature • Research area : o automatic or semi-automatic congresses indexation system o linking GP/FM to the web of data and the linked data initiative The multilingual terminology in GP/FM Out of the online database we are proud to present you the • General Practice / Family Medicine Multilingual Terminology – English version • Terminologia Multilíngue de Clínica Geral / Medicina de Família e Comunidade - Versão em Português • Terminología multilingüe de la medicina general y de familia- Versión española • Terminologie multilingue de médecine générale et de famille – Version française • Een meertalige terminologie in de huisartsgeneeskunde - Nederlandse versie • Từ điển thuật ngữ đa ngôn ngữ về y học tổng quát và y học gia đình – (Vietnamese) • -Codes and definitions will be also available online in Korean and Turkish Those 6 books (60 pages) are readable / downloadable freely online on http://3cgp.docpatient.net/ and contain an explanation of the elaboration process of the taxonomy, a tabular list of the Q-Codes, the list of their definitions and an index. [less ▲]

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See detailRobustness of spatial autocorrelation tests
Ernst, Marie ULg

Poster (2016, November)

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis ... [more ▼]

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis over the classical one. Indeed, the analysis of spatial data is only meaningful if the spatial components bring information. Several measures of spatial autocorrelation are defined in the literature. Moran’s index, Geary’s ratio and Getis-Ord statistic are the most used statistics. Tests based on these measures have been developed in the literature using asymptotic and permutation results. They are used in practice in many fields, for instance in geography, economics, biogeosciences, medicine, ... However, these tests should be cautiously applied because they are not robust. A single contaminated observation can significantly modify their results. This poster is composed of two parts. Firstly, the robustness of already available tools for measuring spatial autocorrelation will be analysed. Secondly, alternative methods will be proposed to robustly test the spatial autocorrelation. [less ▲]

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See detailThe closure of arteriovenous fistula is associated with a significant acceleration of eGFR decline in kidney transplant recipients
Jouret, François ULg; DELANAYE, Pierre ULg; VANDERWECKENE, Pauline ULg et al

Poster (2016, November)

Background The creation of arteriovenous fistula (AVF) may retard chronic kidney disease progression in the general population. Conversely, there is limited literature regarding the impact of AVF closure ... [more ▼]

Background The creation of arteriovenous fistula (AVF) may retard chronic kidney disease progression in the general population. Conversely, there is limited literature regarding the impact of AVF closure on renal function in kidney transplant recipients (KTR). Methods All KTR were retrospectively identified from 01/01/2007 to 31/12/2013, and grouped into: (0) no AVF; (1) closed AVF; and (2) left open AVF. Glomerular filtration rate was estimated (eGFR) upon MDRD and FAS equations. Linear mixed models calculated the slope and intercept of eGFR decline versus time, starting at 3 months post transplantation (Tx). Comparative analyses of eGFR slopes were performed among groups, as well as before vs after AVF closure in group 1. For the latter, time was balanced before vs after AVF closure, with at least 10 observations per patient. Results The cohort included 285 KTR (Table 1), with a median follow-up of 1750 days [1665; 2028]. Focusing on group 1, AVF closure occurred after a mean time of 653 ± 441 days post Tx, with a thrombosis/ligation ratio of 19/95. Balanced study periods before vs after AVF closure lasted 15.7 and 14.9 months, respectively. No difference was found between corresponding intercepts (p, 0.11). By contrast, eGFR slopes were significantly different before (0.043 ml/min/year) versus after (-0.176 ml/min/year) AVF closure (p, 0.0115). Similar observations were obtained using FAS equation Conclusion A significant acceleration of eGFR decline is observed over the 15 months following the closure of functioning AVF in KTR. [less ▲]

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See detailLife cycle assessment of on-site accelerated food waste composter
Groslambert, Sylvie ULg; Léonard, Angélique ULg; Finet, Sébastien

Poster (2016, November)

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See detailComparisons of estimated GFR and Cockcroft Gault equations: calibration against relative risk for all-cause mortality.
Warnock, DG; van den Brand, JAJG; DELANAYE, Pierre ULg et al

Poster (2016, November)

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See detailGlomerular filtration rate in healthy living potential kidney donors: a meta-analysis.
Pottel, H; Hoste; DELANAYE, Pierre ULg

Poster (2016, November)

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See detailL’âgisme chez les étudiants en médecine
Corvol, Aline; Missotten, Pierre ULg; Gentric, Armelle et al

Poster (2016, November)

Introduction: Des stéréotypes négatifs liés à l’avancée en âge sont fréquemment observés en population générale. Des études ont montré que ces stéréotypes sont plus puissants que ceux liés à l’origine ... [more ▼]

Introduction: Des stéréotypes négatifs liés à l’avancée en âge sont fréquemment observés en population générale. Des études ont montré que ces stéréotypes sont plus puissants que ceux liés à l’origine ethnique, au sexe ou à l’orientation sexuelle. Lorsqu’ils sont présents chez les soignants, ces stéréotypes peuvent entrainer des discriminations et limiter l’accès aux soins des personnes âgées (Schroyen et al., 2016). Il a en outre été démontré qu’un comportement d’aide injustifiée pouvait favoriser la perte d’autonomie chez une personne âgée. L’objectif de cette étude est d’évaluer la force des stéréotypes âgistes chez les étudiants en médecine et de rechercher les facteurs qui y sont associés. Méthodes: Un questionnaire écrit informatisé a été proposé aux étudiants en médecine des facultés de Rennes, Brest et Liège, de la 2ème à la 6ème année. Il associe trois mesures : l’Adaptation française de Fraboni Scale of Ageism, une tâche de fluence verbale à propos du vieillissement et de la jeunesse, et une mesure de la stigmatisation liée à l’âge par estimation de pourcentages. Résultats: Des données ont été obtenues pour 570 étudiants à Rennes et 485 à Liège; le recueil de données se poursuit à Brest. Les premières analyses confirment que l’âgisme est largement répandu chez les étudiants en médecine, avec des résultats globalement similaires à Rennes et Liège. Les étudiants de sexe masculins, ceux qui ont peu de contact avec des personnes âgées, ou qui ont des contacts jugés de mauvaise qualité, sont plus susceptibles d’exprimer des stéréotypes âgistes. Le fait d’avoir fait un stage dans un service de gériatrie ou d’avoir bénéficié d’un enseignement de la gériatrie ne semble pas influencer significativement ces stéréotypes. Conclusion: L’enseignement de la gériatrie aux étudiants en médecine doit prendre en compte la fréquence des stéréotypes âgistes. Nos résultats étant soumis à un biais de désirabilité sociale, des études portant sur les stéréotypes implicites (inconscients) seraient utiles. Une meilleure connaissance de l'âgisme et de ses déterminants chez les étudiants en médecine est nécessaire à l'élaboration d'approches pédagogiques susceptibles de limiter les conséquences de ces stéréotypes sur la qualité des soins aux personnes âgées [less ▲]

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See detailBlood meal and blood products detection using Synchronous fluorescence spectroscopy
Lecrenier, Marie-Caroline ULg; Abbas, Ouissam; Taira, Aurélien et al

Poster (2016, November)

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both ... [more ▼]

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both authorised in aqua feed, whereas only porcine blood products are allowed to be used in feed intended for other non-ruminants. Besides official methods (light microscopy and PCR), complementary methods are developed in order to refine the by-products identification. By-products derived from blood are one of these products for which additional information are needed. Indeed, these prohibited materials sometimes cannot be distinguished from those authorised (e.g. milk powder) using official methods. The aim of this work was to develop a fast and easy method to detect blood meal and blood products. This study was based on the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). To achieve this goal, preliminary tests were carried out on reference material (hemoglobin and albumin purchased from Sigma Aldrich) in order to determine appropriate conditions (solvent) and parameters (offset values) for hemoglobin detection. Selected settings were then applied to analyse protein extracts of commercial feed material derived from blood. The results obtained on blood meal and blood products (hemoglobin powder and plasma powder) showed fluorescence spectral bands that characterise hemoglobin. In order to determine whether the method was fit for the purpose, real commercial compound feeds known to contain or to be free from blood products or blood meal were analysed. The Principal Component Analysis (PCA) applied to these spectra showed that it was possible to discriminate samples containing hemoglobin from those that do not contain. This result confirmed that SFS is a promising screening method for the detection of hemoglobin in animal feed. [less ▲]

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See detailPreoptic glutamate and estradiol release during male sexual behavior
de Bournonville, Catherine ULg; de Bournonville, Marie-Pierre ULg; Aourz, Najat et al

Poster (2016, November)

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male ... [more ▼]

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male Japanese quail have also identified following exposure to a receptive female a rapid decrease in the activity of brain aromatase (AA) the enzyme responsible for the conversion of androgens into estrogens. These effects occur mainly within the medial preoptic nucleus (POM), a sexually dimorphic structure of the preoptic area that plays a key role in the activation of male sexual behavior and contains the highest AA in the brain. In vitro studies demonstrated that AA can be rapidly inhibited by calcium-dependent phosphorylations of the enzyme triggered by the activation of AMPA and kainate receptors. We confirmed here this rapid effect of glutamate on AA by injecting kainate in the POM of anesthetized males and measuring AA in the tissue after brain collection. AA in POM was inhibited in the kainate-injected hemisphere compared to the control hemisphere injected with vehicle. In a second experiment, we showed by in vivo microdialysis that glutamate is released in POM during copulation. These results thus suggest that glutamate controls dynamic changes of AA that occur in the brain during sexual interactions. To confirm that the decrease in AA leads to an actual reduction of local estradiol concentration, we quantified via microdialysis and radioimmunoassay changes in estradiol concentration in the male POM during sexual interactions with a female. Surprisingly, a dramatic elevation of estradiol was observed during copulation. Estradiol has been shown to enhance acutely male sexual motivation, therefore the function of its increase in the POM could be to maintain motivation during the entire sexual encounter. The decrease of AA observed ex vivo after copulation would then reflect a compensatory mechanism to restore baseline pre-copulatory conditions. Importantly, these results highlight that although long-term changes in AA are often used as a proxy for local estradiol concentrations, these two measures can show major short-term discrepancies possibly reflecting variations in estrogen turnover. [less ▲]

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See detailSpeech style manipulations in dysarthria in French-speaking children: Acoustic findings.
Ancelle, Josephine; Moya Gale, G.; Hetrick, B et al

Poster (2016, November)

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See detailSpecific detection of blood derived products in animal feed using UPLC-MS/MS
Lecrenier, Marie-Caroline ULg; Dieu, Marc; Veys, Pascal et al

Poster (2016, November)

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly ... [more ▼]

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly regulated. Blood meal and blood products (hemoglobin powder and plasma powder) of bovine origin are totally prohibited. Blood meal and blood products of porcine origin are authorised in aquafeed, whereas only blood products are allowed to be used in feed intended for other non-ruminant. The detection of the type of protein and the species of origin is therefore crucial to ensure feed safety. With the current official methods for the detection of PAPs, light microscopy and PCR, it is not always possible to specifically identify this type of protein source. The objective of our work was to set-up a routine mass spectrometry method for a qualitative detection of blood meal and hemoglobin powder of bovine and porcine origin in feed. The method was based on the detection of species-specific peptides biomarkers identified in a previous study by a non-targeted approach. All biomarkers were peptides of α and β hemoglobin subunits. Proteins were extracted using a TCA/acetone protein precipitation protocol followed by purification with a 2-D Clean-Up Kit (GE Healthcare, USA). After an in-solution trypsin digestion step, analyses were performed by liquid chromatography (Acquity system, Waters, UK) coupled with a triple quadrupole mass spectrometer (Xevo TQS, Waters, UK) with electrospray ionisation. The acquisition and processing of data was carried out by MassLynx software (v. 4.1, Waters) and multiple reaction monitoring (MRM) design was made using the open-source software Skyline (https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view). Reference hemoglobin powder was used to select MRM transitions for each peptide biomarkers and to optimise their collision energy. Commercial feed material and compound feed containing or free from blood derived products were then analysed. Selected transitions were present in materials or feed known to contained blood derived product of the same origin and absent in the others. Artificially contaminated feed with various contamination levels were also analysed in order to evaluate the influence of matrix composition and to experimentally determine the limit of detection. A first estimation of the LOD was around 0.05 % w/w which was below the LOD imposed by the EC for animal proteins detection method. [less ▲]

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See detailThe ‘cyanobiome’ of Svalbard, High Arctic
Stelmach Pessi, Igor ULg; Laughinghouse, H Dail; Velazquez, David et al

Poster (2016, October 28)

Over the last decades, the Arctic has experienced a warming trend that is nearly twice as high as the global average, a phenomenon known as ‘Arctic amplification’. The impact of warmer temperatures on ... [more ▼]

Over the last decades, the Arctic has experienced a warming trend that is nearly twice as high as the global average, a phenomenon known as ‘Arctic amplification’. The impact of warmer temperatures on Arctic ecosystems is still unclear. Cyanobacteria are the key primary producers in freshwater and terrestrial Arctic ecosystems, where they are the driver for numerous ecological functions. For a better understanding of the impacts of climate change on Arctic ecosystems, baseline knowledge on cyanobacterial diversity and distribution is crucial. Here we investigate, for the first time, the biogeographic patterns of cyanobacterial communities across Svalbard, using 454 pyrosequencing of partial 16S rRNA gene sequences. Samples were taken from distinct ecosystems and biogeographic zones. We also compare the studied communities with similar Antarctic communities. [less ▲]

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See detailThe BCCM/ULC collection to conserve the biodiversity and explore the applied potential of Polar cyanobacteria
Becker, Pierre; SZTERNFELD, P; ANDJELKOVIC, M et al

Poster (2016, October 28)

In the Polar Regions, Cyanobacteria represent key primary producers and are the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial ... [more ▼]

In the Polar Regions, Cyanobacteria represent key primary producers and are the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts in terrestrial biotopes. They may present interesting features to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other stresses. The BCCM/ULC public collection funded by the Belgian Science Policy Office since 2011 aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths…). It makes it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, and genomic make-up. It presently includes 226 cyanobacterial strains, with 119 being of Antarctic origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). An ISO 9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. The morphological identification shows that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. The 16S rRNA and ITS sequences of the strains are being characterized. Our results show that the Antarctic strains are positioned into 25 OTUs (sequences with > 97,5% 16S rRNA similarity), and thus, represent a quite large diversity. In addition, cyanobacteria are known to produce a wide range of secondary metabolites (e.g. alkaloids, cyclic and linear peptides, polyketides) with bioactive potential. Among these bioactive metabolites, some display antibiotic, anticancer or antifungal effects. In collaboration with the BCCM/IHEM collection of biomedical fungi, a screening of cyanobacterial strains from BCCM/ULC was performed in order to discover potential new antifungal drugs. The analysis of a first set of methanol extracts from 15 different strains put in evidence the antifungal activity of a Phormidium priestleyi isolate. The latter remains active up to 0.5% (v/v) of fungal culture and was able to inhibit the growth of various fungal species among Candida, Cryptococcus, Aspergillus, and Penicillium. The raw extract was subjected to HPLC and a fraction containing the active molecule was obtained. This molecule appeared to be a thermostable hydrophobic compound. Moreover, in vitro toxicological analyses suggest that the compound has a general cytotoxic effect that could be inhibited by the mammalian metabolism. Further analyses are needed to identify the molecule and to determine if it could be a candidate for a new antifungal drug. In summary, the BCCM/ULC public collection serves as a Biological Resource Centre to conserve ex situ and document the biodiversity of polar cyanobacteria, as well as a repository for discovery of novel bioactive compounds. [less ▲]

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See detailSilurian palynomorphs from the Precordillera basin, western Argentina: biostratigraphy and diversity trends
Garcia Muro, V.J.; Rubinstein, C.V.; Steemans, Philippe ULg

Poster (2016, October 27)

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See detailSpecific interests in Autism with versus without speech onset delay : the importance of perceptually versus thematically organized interests
Chiodo, Liliane ULg; Eusèbe, Sandrine; Majerus, Steve ULg et al

Poster (2016, October 25)

To explore whether autistic adults with versus without speech delay also differ in the perceptual vs thematic nature of their specific interests.

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See detailA benchmark for urban flood modelling
Arrault, Anaïs; Finaud-Guyot, Pascal; Archambeau, Pierre ULg et al

Poster (2016, October 25)

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See detailThermochemical conversion of sugar industry by-products to biofuel
Nicodème, Thibault ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Poster (2016, October 24)

Poster concernant la conversion des co-produits de l'industrie sucrière en biocarburants via la voie thermochimique. Description des procédés tels que la gazéification, Fischer-Trospch, la pyrolyse.

Detailed reference viewed: 29 (4 ULg)