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See detailMises en observation au départ d’un service d’urgences psychiatriques : Procédure, population et orientation.
Schmits, Emilie ULg; Evrard, Maude; Valassopoulou, Eftychia et al

Poster (2017, April 20)

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See detailViolence dans les relations amoureuses : le contexte conflictuel est-il adéquat pour son étude ?
Courtain, Audrey ULg; Glowacz, Fabienne ULg

Poster (2017, April 20)

La violence dans les relations amoureuses des adolescents et jeunes adultes est étudiée depuis un peu plus d’une trentaine d’années (Makepeace, 1981). Ces recherches ont souvent utilisé le questionnaire ... [more ▼]

La violence dans les relations amoureuses des adolescents et jeunes adultes est étudiée depuis un peu plus d’une trentaine d’années (Makepeace, 1981). Ces recherches ont souvent utilisé le questionnaire Conflict Tactics Scale (« CTS 1 », Straus, 1979 ; « CTS2 », Strauss et al., 1996) pour investiguer le phénomène, jusqu’au développement du questionnaire Conflict in Adolescent Dating Relationships Inventory (« CADRI », Wolfe et al., 2001) précisément élaboré pour la violence dans les relations amoureuses. Or, les consignes de passation du CADRI rencontrent celles du CTS en termes de contexte de conflit : « Les phrases suivantes te questionnent sur des situations qui ont pu t’arriver à toi et à ton/ta petit(e) copain/copine pendant que vous vous disputiez ». Le CADRI s’inscrit dans la continuité du CTS eu égard à la théorie du conflit qui leur est sous-jacente. Notre présente recherche entend investiguer comment cette consigne introductive influence les taux de violences agies et subies. En passation groupale, un questionnaire papier a été soumis à des étudiants (N>200, 16-23 ans) en Belgique francophone. Dans un premier temps, les participants ont répondu à une version modifiée du CADRI où la consigne introductive ne circonscrivait pas les items de violence à du conflit ; dans un second temps, ils ont répondu à la version-type délimitant les items de violence à un contexte de conflit. Pour ces deux versions, la perpétration et la victimisation sont étudiées sous cinq formes de violences : verbale/émotionnelle, physique, sexuelle, relationnelle, et par menace. Pour certaines formes de violences agies et subies, les résultats indiquent des différences de score de perpétration et de victimisation selon le type de test (CADRI en version modifiée vs. CADRI en version-type), tantôt pour les participants de sexe féminin, tantôt pour les participants de sexe masculin. Des interactions entre le type de test et le sexe des répondants sont également relevées. Cette étude permet d’interroger la (non) pertinence d’une consigne mentionnant le conflit pour étudier la violence dans les relations amoureuses, consigne ayant d’ailleurs des impacts différents selon le sexe du répondant. En ce sens, délimiter l’étude de la violence dans les relations amoureuses à un contexte de conflit nous met à risque de grandement sous-estimer les prévalences de perpétration et de victimisation, les violences hors-conflit n’étant pas prise en considération. Egalement, considérant les interactions entre type de test et sexe des répondants, il est important de désormais s’assurer que les différentes prévalences relevées pour chaque sexe ne sont plus des différences liées à l’éventuelle compréhension genrée de cette consigne. Enfin, prendre en compte la violence dans les relations amoureuses dans un contexte non-délimité à du conflit permettra d’élaborer de nouvelles stratégies de prévention et de sensibilisation incluant davantage de contextes. [less ▲]

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See detailEvaluation des traits psychopathiques dans une population de sujets schizophrènes : Les limites de la PCL-R
Englebert, Jérôme ULg; Matteucci, Marie; De Page, Louis et al

Poster (2017, April 19)

Notre étude vise à déterminer si la PCL-R (Psychopathy Check List- Revised), destinée à évaluer la psychopathie et utilisée dans le contexte médico-légal, est adaptée à des sujets diagnostiqués ... [more ▼]

Notre étude vise à déterminer si la PCL-R (Psychopathy Check List- Revised), destinée à évaluer la psychopathie et utilisée dans le contexte médico-légal, est adaptée à des sujets diagnostiqués schizophrènes. Notre hypothèse principale est que plusieurs items de l’échelle peuvent être côtés avec le même score et pourtant exprimer une manière d’être et d’agir différente si le sujet est réellement psychopathe ou s’il est schizophrène. Afin d’éprouver notre hypothèse, nous avons sélectionné sept patients présentant soit une schizophrénie, soit une personnalité psychopathiques, soit considérés comme potentiels « héboïdophrènes » (schizophrénie pseudo-psychopathique) à qui nous avons administré la PCL-R. À partir d’analyses qualitatives, nous avons ensuite établi une liste d’items de la PCL-R sensibles à la psychose. Nous observons que 45% des items se révèlent positivement ou négativement influencés par la psychose au-delà de tout trait psychopathique. Sémiologiquement, c’est la présence de la dynamique paranoïde de la schizophrénie qui influence principalement les scores attribués aux items de la PCL-R. Cette recherche suggère des hypothèses concernant l’interaction psychose-psychopathie et son rapport avec des actes de violence. La conclusion de l’étude est que la PCL-R connait des limitations importantes en ce qui concerne son application à une population de sujets schizophrènes. [less ▲]

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See detailTraits psychopathiques dans une population infra-clinique et traitement émotionnel
Englebert, Jérôme ULg; Bral, Laura; Dehon, Hedwige ULg et al

Poster (2017, April 19)

L’intérêt pour la psychopathie infra-clinique n’est pas neuf. Toutefois, les études sur le sujet sont encore peu nombreuses. Notre recherche a consisté à mettre à l’épreuve deux hypothèses. La première ... [more ▼]

L’intérêt pour la psychopathie infra-clinique n’est pas neuf. Toutefois, les études sur le sujet sont encore peu nombreuses. Notre recherche a consisté à mettre à l’épreuve deux hypothèses. La première, concerne la présence de « traits psychopathiques » dans la population tout-venant et la seconde tend à évaluer dans quelle mesure la présence de ces traits influence le traitement émotionnel. Pour tester ces hypothèses, nous avons créé une version « online » du SRP-III (Self Report Psychopathy). Aux soixante-quatre items initialement présents dans cette échelle nous avons ajouté dix-sept items afin d’être en mesure d’également coter la PCL-R (Psychopathy Check List- Revised). Deux groupes de chacun treize participants ont été créés sur base de leur score au SRP-III (un groupe « faibles caractéristiques psychopathique » et un groupe « fortes caractéristiques psychopathiques »). La seconde partie de l’étude consistait en une entrevue durant laquelle nous avons procédé à des mises en situation d’induction émotionnelle. Pour mesurer l’influence de cette induction, trois tâches cognitives étaient administrées aux sujets des deux groupes. La première hypothèse s’est vue confirmée à travers la récolte des données du questionnaire en ligne. Quant à la seconde hypothèse, les résultats statistiques n’indiquent aucune différence significative entre les groupes en ce qui concerne leur score aux tâches cognitives. Il est donc suggéré que la présence de traits psychopathiques n’influence pas le traitement émotionnel. Cette recherche confirme la présence de la psychopathie dans la population tout-venant et semble infirmer, parmi cette population, l’hypothèse d’un déficit du traitement émotionnel. [less ▲]

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See detailExploitation et instrumentation des mémoires
Nossent, Jérôme ULg

Poster (2017, April 03)

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See detailSaisir les reconfigurations des pratiques d'engagement de "publics" en science et technologie
Macq, Hadrien ULg

Poster (2017, April 03)

La gouvernance des sciences et des technologies se trouve actuellement dans une situation de tension entre deux dynamiques : un agenda néo-libéral promouvant l’innovation technologique, d’une part, et un ... [more ▼]

La gouvernance des sciences et des technologies se trouve actuellement dans une situation de tension entre deux dynamiques : un agenda néo-libéral promouvant l’innovation technologique, d’une part, et un mouvement de démocratisation et d’expérimentation collective, d’autre part. Cette recherche vise à analyser les effets de cette tension sur les différentes pratiques d’engagement de « publics » promues par les autorités publiques. Elle poursuit dès lors une double ambition : caractériser les formes émergentes d’engagement de « publics » et les comparer aux précédentes afin de saisir ce que ce changement de pratiques implique pour la gouvernance des sciences et des technologies. [less ▲]

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See detailDes complots partout? Epistémologie du discours conspirationniste
Debras, François ULg

Poster (2017, April 03)

Il n’existe pas d’un côté des « farfelus » qui voient des complots partout et de l’autre, des scientifiques rationnels qui considèrent que les complots sont des divagations. L’adhésion aux théories du ... [more ▼]

Il n’existe pas d’un côté des « farfelus » qui voient des complots partout et de l’autre, des scientifiques rationnels qui considèrent que les complots sont des divagations. L’adhésion aux théories du complot n’est pas un simple oui ou non. C’est un curseur qui indique que chacun se pose des questions et émet des doutes. De plus, l’histoire témoigne de l’existence de multiples complots bien réels que nous ne nions pas (incident de Mukden, incident du golf de Tonkin, affaire des couveuses au Koweit,…). Les théories du complot nous invitent à ne pas croire aveuglément tout ce que nous racontent les médias. Mais, à l’inverse, il ne faut pas non plus déceler des complots partout et analyser un monde « lu à l’envers » où tout ce qui est présenté comme vrai est faux et inversement. [less ▲]

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See detailStable GPR101 over-Expressing Cell Lines As an Invaluable Tool for Functional Studies, Ligand Screening, and the Identification of Deregulated Genes/Pathways in Patients with X-Linked Acrogigantism
Trivellin, Giampaolo; Janjic, Maria; Larco, Darwin et al

Poster (2017, April 02)

Background: GPR101 is an orphan G protein-coupled receptor (GPCR) that is duplicated in patients with X-linked acrogigantism (X-LAG) and over-expressed in their GH- and PRL-secreting tumors. GPR101 is a ... [more ▼]

Background: GPR101 is an orphan G protein-coupled receptor (GPCR) that is duplicated in patients with X-linked acrogigantism (X-LAG) and over-expressed in their GH- and PRL-secreting tumors. GPR101 is a constitutively active GPCR that strongly activates the cAMP pathway. To elucidate the mechanisms through which GPR101 causes GH over-secretion we generated HEK293 and GH/PRL-secreting (GH3) cells with stable GPR101 expression. Methods: Both cell lines were created via direct integration of a human GPR101-coding sequence into their genome. In HEK293 cells this was achieved by transient transfection of a GPR101-expressing plasmid, while GH3 were transduced with GPR101 lentiviral particles. Cells were selected with appropriate antibiotics and the surviving clones expanded. GPR101 expression was quantified by RT-qPCR and immunofluorescence/western blotting. Cell proliferation (MTT assay), cAMP levels (125I-labeled cAMP tracer), and calcium signaling (FURA 2 AM) were determined. RNA was extracted from both cell lines and subjected to RNA-seq. Differential gene expression between control and GPR101-expressing cells and pathway analysis was carried out with the Stirplate and MetaCore softwares, respectively. De-regulated genes were validated by RT-qPCR. Results: High GPR101 expression was achieved in both cell lines and confirmed at the mRNA and protein level. GPR101-expressing cells proliferated at different rates from the respective controls: GPR101-HEK293 cells were slow-dividing, while GPR101-GH3 divided faster. cAMP production was enhanced in GPR101-GH3 and accompained by increased excitability of cells. Differential expression analysis in HEK293 cells revealed several up-regulated and few down-regulated genes. Among the genes with high expression, several were linked to the cAMP pathway: CGA, PCK1, LINC00473 and PDE3A. Enrichment analysis ranked cytoskeleton remodeling and cell cycle regulation (inhibition of G1/S transition) as the most relevant pathways. In GH3 cells most of the genes with a significantly different expression encoded for membrane-localized proteins, among which were ion channels (Trpm8, Kcnj1), GPCRs (Trhr), and calcium sensors (Syt4, Anxa1). Biological processes associated with these genes are: vesicle transport and fusion, cytoskeleton organization, and energy homeostasis. Conclusions: These results show that the intrinsic activity of GPR101 strongly stimulates cAMP production and this in turn facilitates voltage-gated calcium influx. Changes in cAMP/calcium signaling are accompanied with faster/slower cell division depending on the cell type. Accordingly, several genes associated with these and related pathways are differentially expressed. The establishment of these cell lines will be of paramount importance to validate putative GPR101 ligands and to conduct functional studies. [less ▲]

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See detailSystematic flood modelling to support flood-proof urban design
Bruwier, Martin ULg; Mustafa, Ahmed; Aliaga, Daniel et al

Poster (2017, April)

Urban flood risk is influenced by many factors such as hydro-meteorological drivers, existing drainage systems as well as vulnerability of population and assets. The urban fabric itself has also a complex ... [more ▼]

Urban flood risk is influenced by many factors such as hydro-meteorological drivers, existing drainage systems as well as vulnerability of population and assets. The urban fabric itself has also a complex influence on inundation flows. In this research, we performed a systematic analysis on how various characteristics of urban patterns control inundation flow within the urban area and upstream of it. An urban generator tool was used to generate over 2,250 synthetic urban networks of 1 km². This tool is based on the procedural modelling presented by Parish and Müller (2001) which was adapted to generate a broader variety of urban networks. Nine input parameters were used to control the urban geometry. Three of them define the average length, orientation and curvature of the streets. Two orthogonal major roads, for which the width constitutes the fourth input parameter, work as constraints to generate the urban network. The width of secondary streets is given by the fifth input parameter. Each parcel generated by the street network based on a parcel mean area parameter can be either a park or a building parcel depending on the park ratio parameter. Three setback parameters constraint the exact location of the building whithin a building parcel. For each of synthetic urban network, detailed two-dimensional inundation maps were computed with a hydraulic model. The computational efficiency was enhanced by means of a porosity model. This enables the use of a coarser computational grid , while preserving information on the detailed geometry of the urban network (Sanders et al. 2008). These porosity parameters reflect not only the void fraction, which influences the storage capacity of the urban area, but also the influence of buildings on flow conveyance (dynamic effects). A sensitivity analysis was performed based on the inundation maps to highlight the respective impact of each input parameter characteristizing the urban networks. The findings of the study pinpoint which properties of urban networks have a major influence on urban inundation flow, enabling better informed flood-proof urban design. [less ▲]

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See detailBenthic hypoxia and early diagenesis in the Black Sea shelf sediments
Plante, Audrey ULg; Roevros, Nathalie; Capet, Arthur ULg et al

Poster (2017, April)

Marine waters of semi-enclosed seas are affected by a major environmental issue which is oxygen depletion in bottom waters. Deoxygenation is one of the most widespread man-induced consequences which can ... [more ▼]

Marine waters of semi-enclosed seas are affected by a major environmental issue which is oxygen depletion in bottom waters. Deoxygenation is one of the most widespread man-induced consequences which can be catastrophic for living species. Between 1970 and 1990, the benthic compartment of the Black Sea underwent modifications due to the occurrence and increase of hypoxia. Indeed, these changes might cause a deterioration of the structure and functioning of the ecosystems. Nowadays, some regions, such as the north-western shelf, are still affected seasonally by this phenomenon.Within the framework of the BENTHOX project, a biogeochemical study focusing on the early diagenesis is conducted in the Black Sea. It aims (1) to obtain a better understanding of the impact of benthic hypoxia on the diagenetic pathways, (2) to contribute to a new dataset of biogeochemical measurements in the sediments including porewaters. During a cruise (Emblas II – May 2016), on board the RV Mare Nigrum, sediment cores were taken at 4 stations on the Ukrainian shelf. Porewaters were extracted on board the ship using Rhizon technique under N2 atmosphere and will be analyzed for dissolved nutrients and major ions. In addition, sediments were sliced and will be determined for major solid phases and trace element contents. A multi-proxies (biological, sedimentological, mineralogical and geochemical) approach will be used to identify the hypoxic events and to reconstruct the history of bottom hypoxia. The results obtained will be presented and discussed with emphasis on the first outcomes and the major biogeochemical processes involved in the early diagenesis. [less ▲]

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See detailHST observations of Jupiter's UV aurora during Juno's orbits PJ03, PJ04 and PJ05
Grodent, Denis ULg; Gladstone, G Randall; Clarke, John T. et al

Poster (2017, April)

The intense ultraviolet auroral emissions of Jupiter are currently being monitored in the frame of a large Hubble Space Telescope (HST) program meant to support the NASA Juno prime mission. The present ... [more ▼]

The intense ultraviolet auroral emissions of Jupiter are currently being monitored in the frame of a large Hubble Space Telescope (HST) program meant to support the NASA Juno prime mission. The present study addresses the three first Juno orbits (PJ03, 04 and 05) during which HST obtained parallel observations. These three campaigns basically consist of a 2-week period bracketing the time of Juno’s closest approach of Jupiter (CA). At least one HST visit is scheduled every day during the week before and the week following CA. During the ∼12-hour period centered on CA and depending on observing constraints, several HST visits are programmed in order to obtain as many simultaneous observations with Juno-UVS as possible. In addition, at least one HST visit is obtained near Juno’s apojove, when UVS is continuously monitoring Jupiter’s global auroral power, without spatial resolution, for about 12 hours. We are using the Space Telescope Imaging Spectrograph (STIS) in time-tag mode in order to provide spatially resolved movies of Jupiter’s highly dynamic aurora with timescales ranging from seconds to several days. We discuss the preliminary exploitation of the HST data and present these results in such a way as to provide a global magnetospheric context for the different Juno instruments studying Jupiter’s magnetosphere, as well as for the numerous ground based and space based observatories participating to the Juno mission. [less ▲]

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See detailWork environment and work-to-family conflict: Mediating role of work investment.
Babic, Audrey ULg; Stinglhamber, Florence; Barbier, Marie ULg et al

Poster (2017, April)

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See detailTectonic, human and climate signal over the last 4000 years in the Lake Amik record (southern Turkey)
El Ouahabi, Meriam ULg; Hubert, Aurelia ULg; Vander Auwera, Jacqueline ULg et al

Poster (2017, April)

This study investigates the upper sediments infilling the central part of the Amik Basin in Southern Turkey. The Amik Basin is located in a tectonically active area: it is crossed by the Dead Sea Fault, a ... [more ▼]

This study investigates the upper sediments infilling the central part of the Amik Basin in Southern Turkey. The Amik Basin is located in a tectonically active area: it is crossed by the Dead Sea Fault, a major neotectonic structure in the Middle East extending from the Red Sea in the South to the East Anatolian Fault Zone in the North. Continuous human occupation is attested since 6000-7000 BC in the Amik Basin. The study focuses on the sedimentary record of the Lake Amik occupying the central part of the Basin. Our objective is to constrain major paleo-environmental changes over the last 4000 years. The lake has been drained and progressively dried up since the mid-50s. The absence of water column during the summer season allows to collect lacustrine samples along a 5 meter depth trench with a sampling resolution of 1 to 2 cm. Diverse complementary methods were applied to characterize the sedimentary record: i.e. magnetic susceptibility, grain size, organic and inorganic matter by loss-of-ignition, mineralogy by X-ray diffraction and core scanner X-ray fluorescence (XRF) geochemistry. The age of the record is constrained combining radionuclide and radiocarbon datings. Structural disturbances observed in the lacustrine sediments record are linked with major historical earthquakes from the 6th to the 9th century AD due to the Hasipasa Fault rupture. In addition to the tectonic influence, the sedimentary record clearly shows two periods indicating strong soil erosion in the lake catchment: (1) the most recent erosion phase occurs over the Roman period to Present; (2) the oldest one would have occurred during the Late Bronze period. Such changes are most probably related to change in land use. In term of climate influences, the mineralogical and geochemical results allow to evidence variations in chemical weathering conditions in the watershed and lake water level fluctuations, respectively. The clay mineral assemblages attest for significant pedogenesis transformations, especially during the Islamic/Ottoman period. Based on XRF results, an increase in potassium is attributed to a lake development phase during a wet phase An overflow of the Orontes River would be responsible for clay deposition. By contrast, increased calcium and strontium rather correspond to a low lacustrine level and a drier period. The Bronze and Iron/Hellenistic periods are both characterized by low lake level with limited contribution from the watershed. To conclude, our multiproxy study of the Lake Amik allows to decipher between tectonic, human and climate influences over the last 4000 years. Further step would be to compare the Amik record with other regional archives to evidence local and regional events. [less ▲]

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See detailSafety behaviors: the moderating role of trust and support
Laurent, Julie ULg; Chmiel, Nik; Hansez, Isabelle ULg

Poster (2017, April)

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See detailXenogeneic graft-versus-host disease : Impact of Th17 cells
Delens, Loïc ULg; SERVAIS, Sophie ULg; Vrancken, Louise ULg et al

Poster (2017, March 27)

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See detailAnosognosia for behavioral disorder is related to confidence for false self-related memories in Alzheimer’s disease
Mélon, Marlène; Collette, Fabienne ULg; Salmon, Eric ULg et al

Poster (2017, March 24)

Impaired self-awareness (i.e. anosognosia) and altered metacognitive monitoring in memory tasks have both been evidenced in AD. Furthermore, cognitive studies showed disrupted interaction between self and ... [more ▼]

Impaired self-awareness (i.e. anosognosia) and altered metacognitive monitoring in memory tasks have both been evidenced in AD. Furthermore, cognitive studies showed disrupted interaction between self and memory cognitive systems in AD, reducing cognitive advantages of self-related memories (i.e. decreasing self reference effect, SRE) in these patients. However, how anosognosia relates to impaired metacognitive monitoring in AD, particularly for self-related memories, is still an open question. To investigate this question, an ecological memory task based on face-name associations was administrated to 20 healthy older controls (HC) and 20 mild AD patients. The influence of self-reference at encoding was assessed on subsequent name recall and recollective experience, as well as on predictive (feeling-of-knowing, FOK) and postretrieval (judgments-of-confidence, JOC) metacognitive judgments on name recognition in all participants. Furthermore relationships between metacognitive outcomes and awareness of daily cognitive and behavioral impairment was examined in AD. Altered SRE was evidenced in name recall and recollective experience in AD. Patients also showed impaired FOK and JOC, as well as lower awareness of their daily functioning impairment (anosognosia). Nevertheless, differential effect of self on metacognitive judgment in AD and HC was only observed on JOC for false memories. Importantly, anosognosia for behavioral disorders was related to post-retrieval confidence for false recognition of self-related items in AD patients. Thus, anosognosia concerning behavioral disorder and altered post-retrieval monitoring of self-related memories appear to be related in AD. We hypothesized that impaired monitoring of personal false memories may lead AD patients to rely on distorted recent reality when providing judgment about their everyday behavioral functioning. [less ▲]

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See detailDepression is a heterogeneous condition: A cluster-analysis approach
Wagener, Aurélie ULg; Baeyens, Céline; Van der Linden, Martial ULg et al

Poster (2017, March 24)

The heterogeneity of depression (i.e., symptomatology profiles, treatment responsiveness) is more and more evidenced. From a clinical perspective, having a clearer overview of the symptoms’ heterogeneity ... [more ▼]

The heterogeneity of depression (i.e., symptomatology profiles, treatment responsiveness) is more and more evidenced. From a clinical perspective, having a clearer overview of the symptoms’ heterogeneity of depression will help (1) to deeper understand its underlined psychological processes and (2) to tailor clinical treatments (i.e., psychological interventions and/or antidepressant medications). In order to so, an effort to determine subtypes of depression has been developed through a cluster-analysis approach. Andreasen et al. (1980) identified three clusters of depressed patients based on the Schedule for Affective Disorders and Schizophrenia; Schacht et al. (2014) evidenced five clusters based on the Hamilton Depression Rating Scale (i.e., lack of insight, sleep/sexual/somatic, typical MDD, gastrointestinal/weight loss, mild MDD); Hybels et al. (2012) found three clusters of depressive patients based on the Center for Epidemiologic Studies-Depression Scale in community seniors; and Guidi et al. (2011) found two clusters of depressives in medically ill patients based on the Structured Clinical Interview for DSM-IV (i.e., depressed somatizers, irritable/anxious depression). The current study aims at replicating and extending these previous findings in adults presenting depressive symptoms through a cluster-analysis approach. Unlike previous studies, the highlighting of the clusters will be based on the most frequently used assessment of depression, the Beck Depression Inventory-Second Edition (BDI-II). Further, the definition of the clusters will be based on the presence of the depressive symptoms rather than on their severity because symptoms’ severity on the BDI-II can be misrepresentative of the phenomenology of depressive symptoms’ clusters. A sample of 619 adults from community and mental healthcare centers has been recruited. Inclusion criteria were as follows: being a French-speaking adult aged between 18 and 60 years and presenting at least five symptoms on the BDI-II, irrespective of their severity. Data grouping was achieved through a combination of hierarchical (Ward’s method with squared Euclidian distance measurement) and nonhierarchical procedures (K-means cluster analysis), as recommended by recent theoretical trends. A six-cluster solution was evidenced: heavy sleepers (high levels of tiredness, loss of energy and increase of sleep), cognitive depressives (high levels of pessimism, past failures, guilty feelings, self-dislike and worthlessness), affective-somatic depressives (high levels of affective symptoms - loss of interest and pleasure - and somatic symptoms - increase of appetite and sleep, irritability), mild depressives (mild levels of all symptoms), sleepless depressives (high levels of decrease of sleep and tiredness), typical depressives (high levels of all symptoms). Results evidenced the heterogeneity of depressive symptoms, as six different clusters of depressive adults have been found. Two of the clusters identified in the current study are similar to two clusters identified by Schacht et al. (2014) (sleepless depressives are similar to the cluster “sleep/sexual/somatic” because their profile are highly characterized by symptoms related to insomnia and tiredness; mild depressives are similar to the cluster “mild MDD/symptoms” because their profile are characterized by low to average levels on all symptoms). The take-home message of this study is that depression is a heterogeneous condition. Consequently, it is necessary to consider this heterogeneity in order to tailor the psychological intervention. [less ▲]

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See detailRecollection versus familiarity in normal aging and in mild cognitive impairment: Impact of test format
Simon, Jessica ULg; Gilsoul, Jessica ULg; SALMON, Eric ULg et al

Poster (2017, March 24)

If familiarity is better preserved than recollection in aging and in the early stage of Alzheimer's disease (Koen & Yonelinas, 2014), the experimental conditions promoting its use should be beneficial to ... [more ▼]

If familiarity is better preserved than recollection in aging and in the early stage of Alzheimer's disease (Koen & Yonelinas, 2014), the experimental conditions promoting its use should be beneficial to these populations. One condition influencing the relative contribution of recollection and familiarity during recognition is the format of the test. The aim of this study was to investigate the impact of test format on recollection and familiarity in normal aging and in MCI in two tasks where the level of performance is equalized. Seventy young participants, 70 younger-old (55-69 years old), 69 older-old (70-85 years old), and 13 MCIs were presented with forced-choice and yes/no visual recognition memory tasks with the Remember/Know/ Guess paradigm. Young participants had better recognition memory performance than younger-old, who performed better than older-old and MCIs. Recollection and familiarity declined progressively in healthy aging. In MCI, recollection was more affected than familiarity, but patients demonstrated a more liberal use of familiarity. Finally, test format did not influence strongly the results. Young people used recollection more often in the forced-choice task compared to the yes/no task. [less ▲]

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See detailCoarsening dynamics of a granular lattice gas
Opsomer, Eric ULg; Noirhomme, Martial ULg; Ludewig, François et al

Poster (2017, March 23)

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See detailRecognition memory is associated with hippocampal volume in aging population: new evidence from brain imaging study
Narbutas, Justinas ULg; Blanpain, Manon ULg; Van Egroo, Maxime ULg et al

Poster (2017, March 23)

Introduction. The hippocampus is crucial for episodic memory, especially for recollection and pattern separation (i.e., the ability to store similar episodes as distinct memory traces). Episodic memory ... [more ▼]

Introduction. The hippocampus is crucial for episodic memory, especially for recollection and pattern separation (i.e., the ability to store similar episodes as distinct memory traces). Episodic memory declines with aging and this has been associated with hippocampal dysfunction. The main objective of our study was to explore how performance on a recognition memory task designed to assess pattern separation is associated with hippocampal volume in aging population. Methods. Fourteen healthy late middle-aged participants (52-69 years-old) were evaluated on a Mnemonic Similarity Task (MST). In this task, participants study pictures and then have to discriminate between targets, similar lures, and unrelated foil objects. Recognition memory (RM) is assessed as the difference between hits and false alarms to unrelated foils, while pattern separation Bias metric (BPS) is the difference between the rate of ‘‘Similar’’ responses given to the lure items minus ‘‘Similar’’ responses given to the foils. Hippocampal volume was calculated using ASHS software, which uses T1-weighted and T2-weighted MRI to obtain optimal segmentation of hippocampal subfields. Results. Correlation analysis of preliminary data revealed that RM was significantly positively associated with the volume of the left subiculum and left perirhinal area 35, while there were no significant correlations in the right hemisphere. BPS was positively correlated with the volume of right CA2 region, but negatively associated with the volume of right CA3 region, what is more contradictory according to the current literature. No significant link was found between BPS and the volume of hippocampal subfields in the left hemisphere. Conclusion. In a middle-aged population, better visual recognition memory is associated with larger volume of the left subiculum and perirhinal area 35, two regions supporting representation of objects [less ▲]

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See detailRelating pessimistic memory predictions to Alzheimer’s disease brain structure
Genon, Sarah ULg; Simon, Jessica ULg; Bahri, Mohamed Ali ULg et al

Poster (2017, March 23)

Patients with Alzheimer’s disease (AD) show impairment of episodic memory and related metacognitive processes. The present study examined subjective metacognitive judgments preceding objective memory ... [more ▼]

Patients with Alzheimer’s disease (AD) show impairment of episodic memory and related metacognitive processes. The present study examined subjective metacognitive judgments preceding objective memory retrieval and investigated the neural correlates of pessimistic predictions for successfully retrieved memories in AD patients. AD patients and healthy older participants provided predictive judgements on their recognition performance before retrieval of famous (semantic) and recently learned (episodic) names. Correlations between grey matter volume (GMV) in T1 images and behavioural scores were examined with multivariate (PLS) and univariate (GLM) analyses in AD patients. AD patients showed a significant proportion of successful name recognition preceded by pessimistic prediction (Prediction_low_hits) in episodic memory. PLS revealed that behavioural pattern in AD patients was related with a mainly right lateralized pattern of GMV decrease including medial temporal lobe and posterior cingulate cortex, but also right ventrolateral prefrontal cortex (VLPFC). GLM further confirmed that pessimistic prediction negatively correlated with GMV in VLPFC. Thus, impaired monitoring processes (possibly influenced by inaccurate beliefs) allowing inferences about one’s own memory performance are primarily related to decrease GMV in VLPFC in AD patients. [less ▲]

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See detailImpact of the number of alternatives during a forced-choice recognition task on recollection and familiarity in normal aging
Simon, Jessica ULg; Gilsoul, Jessica ULg; Bastin, Christine ULg

Poster (2017, March 23)

Forced-choice recognition tasks are often used to evaluate recognition memory. However, no study have specifically investigate the impact of the number of alternatives on memory performance. Here, we ... [more ▼]

Forced-choice recognition tasks are often used to evaluate recognition memory. However, no study have specifically investigate the impact of the number of alternatives on memory performance. Here, we wanted to determine, on the one hand, if the number of alternatives - two or three - and on the other hand if the degree of similarity between targets and foils have an impact on recognition strategies. Moreover, we investigated how aging interacted with these variables. We recruited 20 young and 20 older participants. During the reconnaissance task, they had to choose, among two or three photographs of faces, the one that was presented previously. Some couples of targets and foils were more similar than others (sharing 60% of common characteristics versus 40%). For each selected item, the participants had to explain what guided their choice via verbal reports. We observed similar performance between the groups for the two-alternative recognition memory task, while young participants had better performance than the older participants in the three-alternative task. Young participants used more often recollection when the similarity between targets and foils was higher, unlike older participants whose rate of recollection was not influenced by target-foil similarity. Both groups used more often familiarity in the two-alternative task, but older participants demonstrated a more liberal bias. Finally, our participants used more often elimination strategies when the similarity is low or when they had to select one item out of three. [less ▲]

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See detailCatalytic functionalisation of starch and amylose using plant oils: towards polymeric materials and composites
Scholten, Philip ULg; Söyler, Zafer; Detrembleur, Christophe ULg et al

Poster (2017, March 19)

Fossil fuels have been the main source of starting materials for the chemical industry over the last century. Especially synthetic polymers rely on this feedstock and are indispensible for mankind’s ... [more ▼]

Fossil fuels have been the main source of starting materials for the chemical industry over the last century. Especially synthetic polymers rely on this feedstock and are indispensible for mankind’s technological advances and status quo. However, a shift to renewable and sustainable resources is needed, as the end of the fossil fuel era is in sight. Biomass presents one possible alternative to fossil fuels, as it consists of a large pool of different chemicals and is inexhaustible. Cellulose, and the closely related starch and amylose, are available cheaply from plants and have favourable properties, such as high strength, thermal stability and biodegradability. However, such poly(saccharides) have been difficult to use directly as materials because of the lack of thermoplasticity, low solubility and high hydrophilicity. Modifications are thus necessary to be able to exploit their advantageous properties. Recently, our group published the catalytic transesterification of maize starch with different plant oils to obtain fully renewable fatty acid starch esters in a sustainable fashion. Polymeric materials with glass transition temperatures (Tgs), soluble in common organic solvents and an increased hydrophobicity were obtained. Compatibilisation tests of the modified starch and amylose with low-density poly(ethylene) (LDPE) and cellulose are being investigated to obtain composites of commodity plastics with improved mechanical properties. [less ▲]

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See detailModulation of NMDA receptor mediated excitatory synaptic currents in dopamine neurons of the substantia nigra.
Destreel, Geoffrey ULg; Seutin, Vincent ULg; Engel, Dominique ULg

Poster (2017, March 17)

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in ... [more ▼]

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in target areas depends on the presynaptic AP firing pattern and is essential to modulate several aspects of behavior such as the control of movement. Bursting activity is mediated by excitatory afferents and specifically by the activation of NMDA receptors (NMDARs). However, the level of activation of NMDARs at these synapses during spontaneous synaptic activity is unknown. We assessed the occupancy of the glycine binding sites of the NMDAR by testing the effects of coagonists and by blocking the uptake of glycine on spontaneous excitatory postsynaptic currents (sEPSCs) in DA neurons of the SNc. Parasagittal midbrain slices (300 – 350 µm thickness) were cut from the brains 16- to 26- days old Wistar rats using a vibratome. Whole-cell recordings were made using warm (~35C°) standard artificial cerebrospinal fluid. sEPSCs were pharmacologically isolated and recorded at +40 and -70 mV. A large NMDAR-sEPSC component was present at +40 mV as revealed by the application of 50 µM D-AP5 (n=8, P < 0.001). At -70 mV, D-AP5 had no effect on the EPSC, as expected (n=7, P = 0.52). The mean amplitude of the average NMDAR sEPSC measured at +40 mV increased significantly from 8.6 ± 0.8 pA in control conditions to 10.9 ± 1.0 pA during bath application of 300 μM glycine (n=10, P < 0.001). We also investigated the effect of D-serine, the other coagonist at the NMDAR glycine site. The mean NMDAR EPSC amplitude measured at +40 mV showed a tendency toward larger values, from 6.8 ± 0.8 pA in control conditions to 8.8 ± 1.1 pA in the presence of 100 µM D-serine (n=4). Blockade of the glycine transporter-1 (GlyT1) significantly increased the mean amplitude of NMDAR sEPSCs measured at +40 mV from 7.1 ± 0.5 pA in control conditions to 10.5 ± 0.8 pA in the presence of 5 μM NFPS (n=8, P < 0.001). At -70 mV, NFPS had no effect on the AMPAR-sEPSC component, as expected (n=8, P = 0.23). In conclusion, our results indicate that the NMDAR glycine sites are not saturated by glycine and D-serine during sEPSCs in control conditions. [less ▲]

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See detailMechanisms of Igf2 inhibition in thymic epithelial cells infected with CVB4 E2
Michaux, Hélène ULg; Charlet-Renard, Chantal; Martens, Henri ULg et al

Poster (2017, March 12)

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See detailPolitiques Architecturales en Belgique
De Visscher, Lisa ULg

Poster (2017, March 10)

The procedures for tenders proposed by the different government architects or the Cellule Architecture in Flanders, Brussels and Wallonia have an important influence on the quality of the process of the ... [more ▼]

The procedures for tenders proposed by the different government architects or the Cellule Architecture in Flanders, Brussels and Wallonia have an important influence on the quality of the process of the project and therefore on its physical result. Comparison and analysis of the different existing procedures. [less ▲]

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See detailLong-range Rydberg-blockade entangling gate mediated by auxiliary atoms
Cesa, Alexandre ULg; Martin, John ULg

Poster (2017, March 09)

Arrays of qubits encoded in the ground state manifold of trapped neutral atoms appear as a promising platform for the realisation of a scalable quantum computer. Indeed, such physical qubits have a long ... [more ▼]

Arrays of qubits encoded in the ground state manifold of trapped neutral atoms appear as a promising platform for the realisation of a scalable quantum computer. Indeed, such physical qubits have a long coherence time and allow for high-fidelity single-qubit operations [1]. In such a platform, entangling two-qubit gates can be implemented by exploiting the Rydberg-blockade mechanism to produce a phase shift or a flip of the state of a target atom conditioned on the state of a control atom [2]. However, because dipole-dipole interactions fall off rapidly with the interatomic distance, such entangling gates based on Rydberg-blockade are impractical between distant qubits. In this work, we propose a protocol to implement long-range Rydberg-blockade gates (CZ or CNot) using auxillary non-coding atoms to transfer the Rydberg excitation from the control to the target qubit. The dependence of the fidelity on the number of auxillary atoms, the blockade strength and the decay rates of the Rydberg states are determined. When compared to a sequential application of nearest neighbours entangling gates, our protocol leads to a larger fidelity and a reduction of the overall gate duration (which scales linearly with the number of auxillary atoms). [1] M. Saffman, J. Phys. B: At. Mol. Opt. Phys. 49, 202001 (2016). [2] D. Jaksch, J. I. Cirac, P. Zoller, S. L. Rolston, R. Côté, and M. D. Lukin, Phys. Rev. Lett. 85, 2208 (2000). [less ▲]

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See detailAnticoherence measures for spin states
Baguette, Dorian ULg; Martin, John ULg

Poster (2017, March 09)

Among all possible spin states, spin-coherent states are the most classical because the spin expectation value in these states yields a vector of maximal norm pointing in a well defined direction. In ... [more ▼]

Among all possible spin states, spin-coherent states are the most classical because the spin expectation value in these states yields a vector of maximal norm pointing in a well defined direction. In contrast, anticoherent spin state to order t are such that <(J.n)^k> is independent of the unit vector n for k = 1, ..., t [1]. By construction, coherent and anticoherent spin states are at both ends of the spectrum of classicality. The aim of this work is to position all possible spin states on such a spectrum, that is to provide measures of anticoherence. To this aim, we introduce an axiomatic definition of anticoherence measures to any order t. In particular, we show that the total variance of a pure spin state, first introduced in [2] can be used to define a measure of anticoherence to order 1. We describe a systematic way of constructing anticoherence measures to any order that relies on the mapping between spin-j states and symmetric states of N = 2j spin-1/2. In particular, we exploit the fact that anticoherent spin states to order t have maximally mixed t-spin-1/2 reduced density matrices in the symmetric subspace [3]. [1] J. Zimba, Electron. J. Theor. Phys. 3, 143 (2006). [2] A. A. Klyachko, B. Öztop, and A. S. Shumovsky, Phys. Rev. A 75, 032315 (2007). [3] D. Baguette, T. Bastin, and J. Martin, Phys. Rev A 90, 032314 (2014). [less ▲]

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See detailUnravelling Potential Roles of HTLV-1 Tax and HBZ Proteins in mRNA Splicing Regulation
Vandermeulen, Charlotte ULg; Cherkaoui, Majid ULg; A. Calderwood, Michael et al

Poster (2017, March 08)

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See detailInvestigating non-coding viral transcripts in Bovine Leukemia Virus induced leukemia
Hahaut, Vincent ULg; Artesi, Maria ULg; Durkin, Keith ULg et al

Poster (2017, March 08)

Bovine Leukemia Virus (BLV) is a deltaretrovirus closely related to the Human T-cell leukemia virus-1 (HTLV-1). The natural host of BLV is cattle and much like the case of HTLV-1 in humans, about ~5% of ... [more ▼]

Bovine Leukemia Virus (BLV) is a deltaretrovirus closely related to the Human T-cell leukemia virus-1 (HTLV-1). The natural host of BLV is cattle and much like the case of HTLV-1 in humans, about ~5% of infected individuals develop leukemia/lymphoma following a long period of asymptomatic infection (~7 years in cattle, several decades in human). Experimental infection of sheep with BLV results in a reduced latency period (2 years on average), making for an attractive cancer model. A further advantage of the BLV system is that it is possible to infect sheep via injection of a cloned provirus, facilitating the mutation of specific parts of the viral genome to examine the function of viral products in vivo. Like HTLV-1, the BLV mRNAs/proteins are transcribed from the viral 5’ long terminal repeat (LTR), a region rich in regulatory elements. It was previously believed that the BLV provirus was transcriptionally silent in tumors, however we identified a cluster of five abundantly expressed non-canonical RNA polymerase III dependent microRNAs (miRNAs) encoded by BLV (Rosewick et al., PNAS 2013). In addition, using RNA sequencing we recently discovered viral antisense transcripts originating in the 3' Long Terminal Repeat (LTR) of the BLV provirus (Durkin et al., Retrovirology 2016) . While 5'LTR dependent transcription is absent in malignant cells, both the viral miRNAs and the antisense transcripts are expressed in all BLV induced leukemic and pre-leukemic samples examined to date, pointing to a vital role in the life cycle of the virus and a critical function in cellular transformation. [less ▲]

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See detailCatalytic reforming of gasification tars with bi- and trimetallic catalysts optimized with organosilane precursors
Claude, Vincent ULg; Lambert, Stéphanie ULg

Poster (2017, March 07)

Biomass gasification is interesting for the production of CO + H2 from wastes and catalysts are necessary for tars degradation. Catalysts were synthesized by the sol-gel process to develop γ-Al2O3 doped ... [more ▼]

Biomass gasification is interesting for the production of CO + H2 from wastes and catalysts are necessary for tars degradation. Catalysts were synthesized by the sol-gel process to develop γ-Al2O3 doped with 10%wt of nickel and 2 wt.% of a second dopant (Co, Cu, Fe, Mn, Mo). Before their adding in AlOOH sol, metallic dopants were complexed with (OCH3)3-Si-(CH2)3-NH-(CH2)2-NH2 (EDAS) to increase their dispersion by cogelation between EDAS and AlOOH clusters. These catalysts were tested at 650°C for toluene reforming. [less ▲]

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See detailCharacterizing exoplanetary atmospheres with a mid-infrared nulling spectrograph
Defrere, Denis ULg; Léger, Alain; Absil, Olivier ULg et al

Poster (2017, March 07)

The discovery of an increasing number of terrestrial planets around nearby stars marks the dawn of a new era in the exoplanet field: the characterization and understanding of their atmospheres. To make ... [more ▼]

The discovery of an increasing number of terrestrial planets around nearby stars marks the dawn of a new era in the exoplanet field: the characterization and understanding of their atmospheres. To make significant progress, it becomes clear that a large number of exoplanetary atmospheres have to be studied at various wavelengths. This is particularly relevant for identifying possible bio-signatures. In this poster, we present a concept of a space-based mid-infrared nulling spectrograph that can characterize a large number of exoplanetary atmospheres and provide key information on their size, surface temperature, and the presence of key molecules such as CO2, H2O, CH4 and O3. The proposed mission concept would be particularly suited to characterize Proxima Cen b. [less ▲]

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See detailThree-dimensional modelling of the Southern Bight of the North Sea: first results and perspectives
Ivanov, Evgeny ULg; Capet, Arthur ULg; Barth, Alexander ULg et al

Poster (2017, March 03)

The impact of offshore wind farm installation and dredging activities on the spatial distribution and dynamics of sediment grain size, biogeochemistry and biodiversity will be estimated in the Southern ... [more ▼]

The impact of offshore wind farm installation and dredging activities on the spatial distribution and dynamics of sediment grain size, biogeochemistry and biodiversity will be estimated in the Southern Bight of the North Sea (SBNS) with a focus on the Belgian Coastal Zone (BCZ) in the frame of the FaCE-It research project (Functional biodiversity in a Changing sedimentary Environment: Implications for biogeochemistry and food webs in a managerial setting). The three-dimensional hydrodynamical model ROMS-COAWST was implemented for simulation of the complex hydrodynamics of SBNS and sediment transport. The first level of nesting with the resolution of 1 km was used in the area of Belgian Economical Zone. In order to reach a fine resolution of 250 m in the BCZ, the second level of nesting will be used. Six-hourly ECMWF ERA-interim meteorological data was used to force the model at the sea-air boundary and the coarse resolution model results available from Copernicus Marine Environment Monitoring Service were used to force the model at the open boundaries. Tides and rivers were also considered. Next types of long-run simulations have been conducted: a 10-years climatological simulation and an interannual simulation over 2004-2013 in order to investigate the interannual dynamics. The model accuracy was evaluated through validation of its outputs against observed salinity, temperature and currents data (remote sensing and in-situ). Results validation of currents and temperature and salinity horizontal fields and vertical profiles against available satellite fields and in-situ data, i.e. from the project field campaign, is conducted and discussed. Application of the nested grid and its benefits for results accuracy is also presented. [less ▲]

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See detailPhotonic photoanode for macroporous perovskite solar cells
Baron, Damien ULg; Dewalque, Jennifer ULg; Henrist, Catherine ULg et al

Poster (2017, March 01)

Organic-inorganic metal halide perovskite solar cells (PSCs) have reached power conversion efficiencies over 20%. Two archetypal PSC architectures are reported in the literature: mesoporous and planar ... [more ▼]

Organic-inorganic metal halide perovskite solar cells (PSCs) have reached power conversion efficiencies over 20%. Two archetypal PSC architectures are reported in the literature: mesoporous and planar PSCs. In the former one, a mesoporous TiO2 scaffold is incorporated into the cell. Because sizes of the mesopores are typically small compared to wavelengths of visible light, the scaffold barely scatters light. In this work, we propose to periodically structure a porous TiO2 scaffold incorporating pores with diameters comparable to wavelengths of visible light thanks to the use of colloidal crystal templating fabrication method. The resulting TiO2 scaffold filled with perovskite will constitute an opal-like photonic crystal incorporated in the solar cell, which will strongly interact with light. Through Finite Difference Time Domain (FDTD) simulations, we demonstrate that the photonic crystal induces resonances that can be exploited to modulate light harvesting in the macroporous TiO2 layer. Sunlight absorption by the PSC will be presented and discussed with dependency of pore sizes and number of opal layers. [less ▲]

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See detailInvestigation of the copolymerization behavior of a cyclic carbonate with vinyl acetate using CMRP
Scholten, Philip ULg; Demarteau, Jérémy ULg; Debuigne, Antoine ULg et al

Poster (2017, March)

Ethylene is an unconjugated monomer difficult to copolymerise in a controlled fashion using common radical or coordination-insertion polymerisation. Cobalt-mediated radical polymerisation (CMRP) provides ... [more ▼]

Ethylene is an unconjugated monomer difficult to copolymerise in a controlled fashion using common radical or coordination-insertion polymerisation. Cobalt-mediated radical polymerisation (CMRP) provides a powerful means to radically copolymerise ethylene in a controlled fashion under mild conditions.[1][2] In addition, different copolymer structures, such as block or alternating, can be accessed. ACO2-based cyclic carbonate was chosen as a promising comonomer but conflicting reports on the copolymer structure have been reported.[3][4] To better understand the copolymerisation behaviour of this monomer, model copolymerisations with vinyl acetate were performed and are reported below with the aim of gaining insights into the copolymersiation behaviour, which will allow us to better tackle ethylene copolymerisations. [less ▲]

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See detailINVESTIGATION DE LA QUALITE D’ANTIBIOTIQUES A BASE D'AMOXICILLINE DANS LE CADRE DE LA SURVEILLANCE DU MARCHE OFFICIEL ET PERIPHERIQUE DE QUELQUES VILLES DE LA R.D. CONGO
Kalenda Tshilombo, Nicodème ULg; Ciza Hamuli, Patient ULg; Mavungu Landu, Don Jethro ULg et al

Poster (2017, March)

Drug counterfeiting is a sad and worrisome reality, especially in developing countries where quality control is not effective and sometimes not existing at all despite political will of governments. The ... [more ▼]

Drug counterfeiting is a sad and worrisome reality, especially in developing countries where quality control is not effective and sometimes not existing at all despite political will of governments. The consequences are harmful in particular for substandard medicines that pose more threats to populations in those countries due to their direct negative impact on patients such as failure of medical treatment including development of drug resistance and even death. Socio-economic consequences and negative reputation concerning the pharmaceutical industry are also observed. Unfortunately accurate detailed data on such medicines are not easy to obtain. Most of the time available data are often estimated from case reports or studies carried out in a specific area and during a defined period.Health authorities’ in the Democratic Republic of Congo are trying to identify this scourge by set up several strategies to fight against. One of them is built on the best knowledge of drugs from several horizons through the assessment of their quality to allow appropriate measurement. In this context, we have focused our study towards amoxicillin alone and/or combined with potassium clavulanate since it is one the very used medicines in pediatric medications. The formulations are powder for suspension. Two analytical methods were developed based on the USP monography, applying isocratic liquid chromatography. Prior to their application in routine, we evaluated the suitability of these methods through validation applying the accuracy profile of total error. Since it was planned to transfer the methods to DRC, several operating factors were taken into account namely operator, day and equipment. Interesting results were obtained in terms of trueness (relative biases below than 2.3%), precision (RSD of Intermediate precision below 2.8%), accuracy (beta-expectation tolerance intervals between -6.0% and 3.8%) for the concentration levels of interest. The latter were able to allow monitoring the quality of the two active ingredients here above in the 65 samples from Congolese market. They were collected in Kinshasa, Lubumbashi, Matadi and Kolwezi at official and non-official medicines distributors, in peripheral area. The dramatic results obtained confirm that substandard and counterfeit medicines remain a crucial problem on public health in low-income countries. Appropriate measures are really needed to set up the drug quality improvement. [less ▲]

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See detailAging and Recollection: a context story
Folville, Adrien ULg; Bastin, Christine ULg; Willems, Sylvie ULg

Poster (2017, March)

Although aging is related to decline in recollection as measured by so-called objective measures, older adults’ subjective experience of recollection remains sometimes stable. Such dissociation could ... [more ▼]

Although aging is related to decline in recollection as measured by so-called objective measures, older adults’ subjective experience of recollection remains sometimes stable. Such dissociation could suggest that younger and older adults use details with different diagnosticity to make subjective recollection judgments. However, the type of details that are reported as bases for recollective experiences by younger adults can also vary as a function of context. Here, we directly investigated age-related changes in recollection and familiarity in different memorability context. Participants studied one set of words in a medium level of processing (LOP) task, and another set of words with either a shallow or deep LOP task (i.e., low vs. high memorability context, respectively). At test, participants discriminated between old and new words and provided information about the basis of their recollective experiences. In both age groups, medium items received more recollection judgments in low (vs. high) memorability context. These recollections seem to be associated with internal information (thought, image, emotion). In contrast, external details (list source, appearance, list position) more often accompanied recollection of medium items in high (vs. low) memorability context. We discuss this effect in terms of Gruppuso et al.’s (1997) functional account. Like younger adults, what older adults deem to be an experience of remembering arises from the functional utility of the recollected information for accomplishing the task. In the low (vs. high) memorability context, the information recollected for medium items more easily met the functional definition of remembering established by participants during the test. [less ▲]

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See detail2 Years outcome of patients in unresponsive wakefulness syndrome/vegetative state and minimally conscious state
Cassol, Helena ULg; LEDOUX, Didier ULg; Thibaut, Aurore ULg et al

Poster (2017, March)

INTRODUCTION: Following severe acute brain damage, patients typically evolve from coma to an unresponsive wakefulness syndrome/vegetative state (UWS/VS; wakefulness without awareness) and later to a ... [more ▼]

INTRODUCTION: Following severe acute brain damage, patients typically evolve from coma to an unresponsive wakefulness syndrome/vegetative state (UWS/VS; wakefulness without awareness) and later to a minimally conscious state (MCS; fluctuating but consistent nonreflex behaviors). MCS is subcategorized in MCS+ (i.e., command following) and MCS- (i.e., visual pursuit, localization of noxious stimulation or contingent behaviours). Reliable and consistent interactive communication and/or functional use of objects indicate the next boundary – emergence from MCS (EMCS). To date, there is still no reliable predictive model of recovery from the UWS/VS and the MCS. A better understanding of patients' outcome would help in decisions regarding patients’ care and rehabilitation, as well as end-of-life decisions. METHODS: We collected demographic information, acute care history and longitudinal follow-up of patients in UWS/VS and MCS admitted in 15 expert centers in Belgium (via the Belgian Federal Public Service Health). Patients were evaluated at 1, 3, 6, 12 and 24 months post injury with the Coma Recovery Scale-Revised and the diagnosis was based on internationally accepted criteria of UWS/VS, MCS or EMCS. Results were considered significant at p<0.001. RESULTS: 24 months follow-up was available for 476 patients including 261 diagnosed in UWS/VS (88 traumatic, 173 non-traumatic) and 215 diagnosed in MCS (80 traumatic, 135 non-traumatic) one month after the injury. Patients who were in MCS one month after the insult were more likely to recover functional communication or object use after 24 months than patients in UWS/VS. Moreover, functional recovery occurred more often in MCS+ (79%) as compared to MCS- (29%), and mortality rate was more important in MCS- patients (68%) as compared to MCS+ (21%). Comparisons within UWS/VS and MCS groups based on etiology showed that traumatic patients had a better outcome at 24 months than non-traumatic patients. Among non-traumatic patients, no difference was found between anoxic patients and patients with other etiologies regarding functional recovery. CONCLUSION: Our study highlights that the outcome is significantly better for patients who are in MCS one month post-injury as compared to patients who remain in UWS/VS at that time. Concerning MCS patients, the outcome is significantly better for patients who are MCS+ one month post-injury as compared to patients who are MCS- at that time. This study also confirms that patients with traumatic etiology have better prognosis than patients with non-traumatic causes. [less ▲]

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See detailMassivement histologique : entre continuité et (r)évolution pédagogique. Lancement du premier Massive Open Online Course (MOOC) sur des travaux pratiques en Histologie à destination de nos étudiants mais aussi du grand public.
Multon, Sylvie ULg; Pesesse, Laurence ULg; Weatherspoon, Alodie ULg et al

Poster (2017, March)

L’acronyme anglophone MOOC désigne les « Massive Open Online Courses » que l’on pourrait traduire par « formation en ligne massive ouverte à tous ». Nés aux États-Unis avec un programme sur l’intelligence ... [more ▼]

L’acronyme anglophone MOOC désigne les « Massive Open Online Courses » que l’on pourrait traduire par « formation en ligne massive ouverte à tous ». Nés aux États-Unis avec un programme sur l’intelligence artificielle dispensé par l’université de Stanford, ces cours en ligne accessibles gratuitement font miroiter la possibilité d’une démocratisation maximale des savoirs. A ce titre, les MOOC bénéficient des innovations issues du Web 2.0 : ils tirent le meilleur de la rencontre entre les nouveaux usages induits par les réseaux sociaux et les apports de la pédagogique active. Ces cinq dernières années, nous avons dispensé les travaux pratiques en Histologie aux premières années d’étude de la faculté de Médecine de l’Université de Liège par une méthodologie hybride alliant les bénéfices d’un enseignement à distance et ceux de séances menées en face-à-face (Multon et al., 2015). Notre dispositif d’auto-apprentissage invite chaque année plus de 500 étudiants à parcourir plusieurs coupes d’organes digitalisées afin de découvrir les structures histologiques pointées par des balises interactives pour chaque grande famille de tissus. La mise en ligne d’un MOOC dédié aux travaux pratiques d’Histologie s’inscrit dans une suite logique de nos activités avec une volonté de donner accès à nos ressources numériques à un public plus large. « Découvrir les tissus fondamentaux du corps humain en explorant par vous même des lames histologiques au microscope », tel est le programme de notre MOOC intitulé « Introduction à l’histologie, exploration des tissus du corps humain ». Celui-ci a débuté en février 2017 et est hébergé sur la plate-forme FUN encadrée par le ministère de l’Enseignement supérieur et de la Recherche du ministère français (https://www.fun-mooc.fr/courses/ulg/108001/session01/about). Ce MOOC s’adresse à un public large : aux étudiants ou futurs étudiants dans le domaine médical, paramédical ou scientifique, aux enseignants, chercheurs, professionnels dans le secteur de la santé, décideurs dans le domaine de l’éducation ou de la santé ou simplement aux curieux désireux de comprendre à partir de quoi le corps humain est construit. Deux parcours pédagogiques permettant d’approfondir différemment la matière sont proposés pour correspondre au mieux aux objectifs ou motivations de chacun. Au travers de vidéos explicatives contenant des dessins réalisés sous vos yeux et des liens avec l’anatomie suivie d’activités interactives telles que la manipulation d’un microscope virtuel, de nombreux quiz et d’activités réalisées avec les pairs, les participants seront capables de reconnaître les différents types de cellules et tous les éléments qui les entourent afin de comprendre comment ils s’organisent entre eux pour former des tissus aux fonctions spécifiques. Une recherche est actuellement en cours sur l’étude des traces d’activités des participants au MOOC en fonction du parcours pédagogique choisi afin d’évaluer notamment l’impact des différentes composantes de l’expérience d’apprentissage. [less ▲]

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See detailNeural correlates of subsequent recollection and familiarity in ageing
François, Sarah ULg; Angel, Lucie; Salmon, Eric ULg et al

Poster (2017, March)

It is now commonly accepted that, in ageing, recollection processes are impaired and familiarity seems to be relatively preserved. In this study, we were interested in the differences between younger and ... [more ▼]

It is now commonly accepted that, in ageing, recollection processes are impaired and familiarity seems to be relatively preserved. In this study, we were interested in the differences between younger and older participants regarding cerebral activity during encoding depending on whether the item later elicited recollection or familiarity. Twenty young volunteers and 19 older volunteers were presented visual stimuli depicting objects. After a first fMRI session in which the items were encoded, they underwent a recognition memory task in which they judged whether items were Remembered, Familiar or New. Data were analysed using SPM8, with an event-related design comparing modifications in cerebral activity between the two subjects groups during encoding (1) for the items leading to recollection compared to those leading to familiarity, and (2) for the items associated with familiarity compared to those which were not recognized. Results showed that older adults displayed a heightened activity in regions of the default-mode network for subsequent recollection. As it does not appear to be deleterious, it could stem from a more frequent use of self-referential appraisal as an encoding strategy in older adults. Furthermore, they show supplementary contralateral activations in frontal and temporal regions, which could be indicative of dedifferentiation or attempted compensation. To conclude, it appears that despite relying less on elaborative encoding, older adults seem to be able to perform recollection using qualitatively different strategies than young adults. [less ▲]

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See detailProteomic differential distribution of 53BP1 in serrated and conventional adenomas validated by histological characterisation
QUESADA-CALVO, Florence ULg; Merli, Angela-Maria ULg; MASSOT, Charlotte ULg et al

Poster (2017, February 10)

INTRODUCTION: Sessile serrated adenoma/polyp (SSA/p) is a precancerous lesion, mostly located in the right side of the colon (cecum, ascending and transverse colon). The difficulty is to visualize this ... [more ▼]

INTRODUCTION: Sessile serrated adenoma/polyp (SSA/p) is a precancerous lesion, mostly located in the right side of the colon (cecum, ascending and transverse colon). The difficulty is to visualize this lesion during colonoscopy because of its subtle appearance. MATERIAL AND METHOD: We compared proteomes of serrated polyps (SSA/p) and conventional adenomas using residual human formalin fixed paraffin embedded (FFPE) samples. FFPE-FASP method was applied on samples before label free proteomic analysis. Immunohistochemistry (IHC) characterisation of one candidate marker was performed for tissue validation on an independent set of samples including: conventional adenomas (low and high-grade dysplasia), serrated polyps (hyperplastic polyps, SSA/p and traditional serrated adenoma) and finally normal colon (taken at the margin of colorectal cancer (CRC) or of diverticular disease). RESULTS: Proteomics provided 765 proteins (out of 5992 proteins identified) significantly discriminating conventional adenomas from serrated lesions. We selected 53BP1 (Tumor suppressor p53-binding protein 1) among these for IHC validation, because of its tumor suppressor gene function and role as a mediator of DNA damage checkpoint. 53BP1 appeared significantly up-regulated in proteomes of low and high grade adenomas compared to these of normal tissue and SSA/p. 53BP1 IHC signal was located in the nucleus and the percentage of positive nucleus decreased in serrated polyps, especially in crypts and in the border epithelium, confirming part of the proteomic results. CONCLUSION: This study highlights potential marker proteins, including 53BP1 from which IHC signal was strongly decreased in some serrated polyps. The loss of 53BP1 has been associated with tumour progression and poor prognosis, while little is currently known about its involvement in precancerous CRC lesions. 53BP1 decrease of expression in the nucleus and therefore possible loss of function in some epithelial cells could reflect important changes occurring during dysplasia to neoplasia progression in serrated lesions. [less ▲]

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See detailNon-visual effect of light on cognitive brain function: Impact of lens yellowing in aging
Daneault, Véronique; Dumont, Marie; Massé, Eric et al

Poster (2017, February 10)

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See detailLab Pay(S)age: a Laboratory of Observation as an activator for Landscape Awareness Process
Baldin, Elisa ULg; Occhiuto, Rita ULg

Poster (2017, February 09)

The notion of Landscape Observatory (ELC 2000), in the work of LabVTP at the Faculty of Architecture in the University of Liège (Wallonia-BE), has been linked with the concept of Landscape Laboratory (R ... [more ▼]

The notion of Landscape Observatory (ELC 2000), in the work of LabVTP at the Faculty of Architecture in the University of Liège (Wallonia-BE), has been linked with the concept of Landscape Laboratory (R. Gustavsson) in order to enhance and underline perceptive, experiential and active dimensions of sites, responding to the Universities’ missions required by the ELC: education and sensitization. Lab Pay(s)age, as a new typology of landscape laboratory, has developed methodological tools for landscape research, based on a multi-scale and diachronic reading of the territory, where natural and artificial traces are the complementary elements to be reinterpreted and relaunched in new participative scenarios. The Meuse valley, reshaped by deep transformations, is the main topic for researches, concerning specific morphological, typological and social characters. All along the river, the scars of industries mark the territory as a land in search of new cycle of life1. The regeneration of abandoned industrial sites, as a practice of territorial recycle, is part of sustainability policies in urban planning, based on the enhancement of local resources. The Observatory Lab Pay(s)age, is intended as a structure for site-specific study and continuous fieldwork, concerning both the evolutionary processes of natural structures and the involution of built environment. Starting from these materials, many different regeneration methodologies and temporalities have been imagined. Following the logic promoted by the ELC, the work has started by monitoring some specific areas and by contacting different local representatives with the aim of collecting data and experiences to construct a shared knowledge of the places. Then the project has become an on-going tool including all phases in the regeneration process: observation, analysis, interpretation and re-launch of new hypothesis are fundamental elements to increase the public knowledge about the cultural factors characterizing the landscape discipline. Thus, the notion of « observatory » is a strategic device, monitoring the evolution of places in time through actions of dialogue and awareness raising, in order to get citizens re-acquire a consciousness of their territory. The collected qualitative data, describe the peculiar features of the sites, based on the local actors experience and knowledge. These elements are the basis for new scenarios, used as progressive steps leading the recovery process. For this reason, the « observatory » is also a « laboratory », as the experimental projects accompany the transformation of the places through intermediate phases. Understanding and accepting mutability as a character of landscape is a preliminary step towards a co-action, where the project accords with natural dynamics. Lab Pay(s)age, according to the ELC principles, is using the earliest local answers as re-launch factors for consecutive projects, taking into account the territorial temporalities as priority in transformation process. The presentation concerns the ongoing projects and obtained reactions, such as the earliest re-naturalization programs, some selected sites for monitoring activities and Phd researches on new regeneration methods using vegetation as an active element in reclamation of industrial sites. 1 Occhiuto, R. 2016. Healing the scars of industry in Liège, in Scape n.15, 2016 [less ▲]

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See detailImportance of using Geographic Information System for extensive Middle Palaeolithic open air sites in northern France. The example of Caours (Somme, France) and Beauvais (Oise, France).
Moreau, Gwénaëlle ULg; Locht, Jean-Luc; Patou-Mathis, Marylène et al

Poster (2017, February 09)

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement ... [more ▼]

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement, raising complication in the study of its dynamics at a regional scale. Therefore, some aspects of Neanderthals behaviour are still mischaracterize: in most cases, the function of the site and its territorial management over the northern France have to be specified. The spatial analysis of open air sites from northern France will help us to answer those questions. However, for Middle Palaeolithic sites, we can't see systematically on the field the spatial organisation directly, we sometimes need modelization. Therefore, we started to build a spatial analysis protocol adapted to this site and applied to such sites. The sites of Caours (Somme, France) and Beauvais (Oise, France) are two open air sites that are exceptionally well preserved and displaying a large amount of faunal and lithic rests. Both are perfect candidate to apply and test the new protocol. First results proved that for each site the existence of a spatial organisation as remains concentration zones. Then, we characterize this areas – number, distribution. Finally, we were able to associate them to human activity areas like hearths, butchery or knapping areas. [less ▲]

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See detailPeptidoglycan fragments separation and identification by zwitterionic hydrophilic interaction chromatography and capillary electrophoresis coupled to mass spectrometry
Boulanger, Madeleine ULg; Raymackers, Alice ULg; Delvaux, Cédric ULg et al

Poster (2017, February 08)

Bacterial peptidoglycan-derived peptides and muropeptides are soluble unique fragments acting as messengers in diverse cell-signalling events. As the bacterial peptidoglycan wall is a major target of ... [more ▼]

Bacterial peptidoglycan-derived peptides and muropeptides are soluble unique fragments acting as messengers in diverse cell-signalling events. As the bacterial peptidoglycan wall is a major target of antibiotics, bacteria have developed specific resistance mechanisms based on the detection of such fragments. In addition, the muropeptides sensing is involved in the innate immune response toward bacterial invasion and is therefore of major importance in the eukaryotes self-defence functions. In Bacillus licheniformis 749/I, the peptidoglycan dipeptide m-A2pm-D-Glu triggers beta-lactam resistance via the induction of a beta-lactamase, BlaP. This induction process relies on a complex regulation system for which the nature and the concentration of peptidoglycan fragments leading to the formation of dipeptide moiety inside the cytoplasm are unknown. In this context, the development and the validation of a reliable method to identify and quantify those cytoplasmic fragments is of major interest. Conventionally, the peptidoglycan is first digested by mutanolysin in order to generate muropeptides which are subsequently analyzed by reversed-phase liquid chromatography (RP-LC, C18). However, this technique is not effective enough to separate the peptides that, as a result, are eluted in the flow through . In this work, we developed two novel analytical separation methods, namely capillary electrophoresis (CE) and zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) both coupled to mass spectrometry (MS), aiming at overcoming the drawbacks encountered in traditional separation techniques. Both methods show great results in the identification of peptidoglycan fragments in complex samples. CE analysis lead to muropeptides and peptides separation whereas ZIC-HILIC only retains peptides. Nevertheless, the latter has been optimized and validated for the cytoplasmic peptidoglycan peptides identification and quantification. Althogether, ZIC-HILIC-MS and CE-MS have proved to be powerful analytical tools for the identification and quantification of peptidoglycan fragments in complex matrix samples. Further optimizations are still ongoing for the analysis of muropeptides, which hopefully will lead to the identification and quantification of cytoplasmic peptidoglycan fragments composition during the Bacillus licheniformis 749/I BlaP beta-lactamase induction process. [less ▲]

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See detailComparison of two laboratory-scale ensiling methods to assess effect of reducing sugars
Herremans, Sophie ULg; Decruyenaere, Virginie; Beckers, Yves ULg et al

Poster (2017, February 07)

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See detailLoss of transfer RNA U34 modifying enzymes impairs hematopoietic stem and progenitor cell differentiation and function
Rosu, Adeline ULg; Bai, Qiang ULg; Ramery, Eve ULg et al

Poster (2017, February 03)

Hematopoietic stem and progenitor cells (HSPCs) require fine-tuned protein translation for their normal maintenance and function. Conserved modifications of the wobble uridine base (U34) in transfer RNAs ... [more ▼]

Hematopoietic stem and progenitor cells (HSPCs) require fine-tuned protein translation for their normal maintenance and function. Conserved modifications of the wobble uridine base (U34) in transfer RNAs catalyzed by the Elongator complex are required for optimal protein translation efficacy and fidelity, but their biological importance in mammalian stem and progenitor cells remains largely unexplored. Here, we studied the impact of loss of activity of the catalytic subunit Elp3 of Elongator on HSPC differentiation and function. Hematopoietic-cell-specific depletion of Elp3 in conditional knockout mice resulted in shortened lifespan associated with hematopoietic failure and lymphoma development. Elp3 deletion caused apoptosis of specific bone marrow multipotent progenitors and blocked differentiation of committed progenitors, resulting in blood and bone marrow pancytopenia. In contrast, Elp3-deficient hematopoietic stem cells (HSCs) expanded with age and did not exhaust throughout life, although they were defective in reconstituting hematopoiesis in competitive transplantation assays. Mechanistically, loss of Elp3 did not result in detectable alterations in global protein synthesis rates in any HSPC subset. Rather, Elp3-deficient HSPCs displayed enhanced activity of the stress integrator and apoptosis and cell cycle regulator p53. Thus, this study supports the notion that Elongator activity is required in distinct HSPC subsets to avoid aberrant p53 activation, which otherwise results in discrete loss of function phenotypes in HSCs and downstream progenitors. [less ▲]

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See detailModelling BMP-2 carrier device for bone tissue engineering application
Manhas, Varun ULg; Carlier, Aurelie; Geris, Liesbet ULg

Poster (2017, February 01)

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See detailUsing unsupervised clustering method and SNP-based information to identify fine-level population structure
Chaichoompu, Kridsadakorn ULg; Yazew, Fentaw Abegaz; Tongsima, Sissades et al

Poster (2017, February 01)

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See detailCellular stress and β-lactamase BlaP induction in Bacillus licheniformis
Dauvin, Marjorie ULg; Joris, Bernard ULg

Poster (2017, February 01)

In presence of β-lactam antibiotic in its environment, B. licheniformis produces the β-lactamase BlaP, an enzyme hydrolyzing β-lactam antibiotic, conferring on the bacteria phenotypic resistance. To ... [more ▼]

In presence of β-lactam antibiotic in its environment, B. licheniformis produces the β-lactamase BlaP, an enzyme hydrolyzing β-lactam antibiotic, conferring on the bacteria phenotypic resistance. To induce BlaP, two conditions must be fulfilled. The first one is the acylation of the membrane receptor BlaR1 by the antibiotic. The second one is a cellular stress due to the presence of the antibiotic which acylate PBP1. The nature of this signal remains unknown. In this study we postulate that the extracytoplasmic function sigma factors (ECFs) σM and σX act together with the stringent response as a secondary and redundant layer of stress upon which the BlaP induction pathway relies. [less ▲]

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See detailMyoferlin, a new autophagy player in pancreatic cancer cells
Rademaker, Gilles ULg; Hennequière, Vincent ULg; Peixoto, Paul et al

Poster (2017, February 01)

Despite intensive research, Pancreatic Ductal Adenocarcinoma (PDAC) remains one of the deadliest forms of cancer. Early-stage of the disease is clinically silent and the diagnosis of the disease is mostly ... [more ▼]

Despite intensive research, Pancreatic Ductal Adenocarcinoma (PDAC) remains one of the deadliest forms of cancer. Early-stage of the disease is clinically silent and the diagnosis of the disease is mostly made at an advanced stage. This late diagnosis contributes to one of the lowest 5-year survival rates (<5%). Today, PDAC are treated by surgery and/or adjuvant therapy, increasing only slightly the median survival of the patients. There is therefore an urgent need to develop new effective therapies for PDAC patients. PDAC are characterized by a high autophagic activity involved in its chemoresistance. Recently, key regulatory proteins controlling the metabolic reprogramming of PDAC cells were identified. By governing both autophagic flux and lysosomal catabolism, these proteins support the efficient processing of cargo from autophagy, providing PDAC cells with access to critical sources of nutrients. Interestingly, the high autophagy level in PDAC correlates with a poor patient outcome. Myoferlin, a member of the ferlin family overexpressed at protein level in different cancer types including PDAC, is a transmembrane protein able to bound to phospholipids and described to play an important function in membrane fusion. This characteristic invited us to investigate whether myoferlin could participate to autophagy, a process involving membrane fusion. Panc-1 cell line was used as a model of PDAC basal autophagy. Myoferlin expression was silenced using interfering RNA technology. Autophagosome abundance was evaluated by LC3-II western-blot and flow cytometry. Results indicated a significant increase in autophagosome abundance 48 h after myoferlin-silencing. This increase could arise from an increase of autophagy initiation or from an inhibition of autolysosome degradation. Using autophagy inhibitors, autophagic flux was evaluated by LC3-II and p62 western-blot after myoferlin-silencing. Results suggested a blockade in the autophagic process, impairing termination and autophagosome degradation by lysosome activity. Knowing the affinity of myoferlin for phospholipids, we wonder if this protein could interact with the phosphatidylethanolamine-conjugated LC3-II protein. Proximity-ligation assay suggested a close interaction between myoferlin and LC3. These results evoke an unexplored and undescribed role for myoferlin in autophagy. Understanding the involvement of myoferlin in this rediscovered biological process could give new clues in the development of new therapeutic strategy. [less ▲]

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See detailCrosstalk between VEGF and BMP9 pathway. Implications in HHT disease
Pollenus, Thomas ULg

Poster (2017, February 01)

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See detailIdentification of proteins discriminating inflammation induced dysplasia from simple inflammation in ulcerative colitis by laser capture microdissection and label free proteomics – a pilot study
Merli, Angela-Maria ULg; QUESADA-CALVO, Florence ULg; MASSOT, Charlotte ULg et al

Poster (2017, February 01)

Chronic colonic inflammation in ulcerative colitis (UC) may induce dysplasia, which can itself progress and transform into neoplasia. Diagnosis of dysplasia in UC remains difficult particularly when ... [more ▼]

Chronic colonic inflammation in ulcerative colitis (UC) may induce dysplasia, which can itself progress and transform into neoplasia. Diagnosis of dysplasia in UC remains difficult particularly when tissue inflammation is present. The aim of this retrospective pilot study was to highlight proteins specifically associated with inflammation induced dysplasia in UC. We performed a pilot experiment on 15 Formalin-Fixed, Paraffin-Embedded (FFPE) samples isolated from 5 cases of UC patients with a Polypoïd Pedunculated dysplasia (UC-PP). We compared the proteomes of the UC-PP, the inflammatory (UC-I) and the normal (UC-NL) tissues of each patient. We performed Laser Capture Microdissection (LCM) in order to collect only epithelial cells, avoiding inflammatory infiltrating ones. Label free proteomic analysis using a 2D-nanoUPLC coupled with a hybrid Quadrupole-Orbitrap was applied, as well as differential analysis on the paired samples. Immunohistochemistry (IHC) characterisation of one of the selected proteins of interest was used for validation. Out of 985 quantified proteins, 7 were found significantly more abundant in UC-PP compared to UC-I tissues, with 6 being only detected in UC-PP using proteomics. One of these is Solute Carrier Family 12 member 2 (SLC12A2), also known as Na-K-2Cl co-transporter 1 (NKCC1), a protein involved in ionic balance, in T-cell migration promotion and in some features involved in cancer development like proliferation, migration or invasion. IHC results obtained were in correlation with proteomic results and showed that SLC12A2 was more abundant in UC-PP tissue than in UC-I and UC-NL tissues, with a signal clearly delimiting the dysplastic region from the surrounding inflammatory tissue. This pilot experiment shows a different proteomic profile in inflammation-associated dysplasia and simple inflammation. This should be replicated using other types of dysplasia in IBD. SLC12A2 could be a potential biomarker of inflammation-associated dysplasia. [less ▲]

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See detailImproved Blood Glucose Forecasting Models using Changes in Insulin Sensitivity in Intensive Care Patients
Uyttendaele, Vincent ULg; Dickson, Jennifer; Shaw, Geoff et al

Poster (2017, February 01)

Introduction: Hyperglycaemia, hypoglycaemia and glycaemic variability are associated with worsened outcomes and increased mortality in intensive care units. Glycaemic control (GC) using insulin therapy ... [more ▼]

Introduction: Hyperglycaemia, hypoglycaemia and glycaemic variability are associated with worsened outcomes and increased mortality in intensive care units. Glycaemic control (GC) using insulin therapy has shown improved outcomes, but have been proven difficult to repeat or achieve safely. STAR (Stochastic TARgeted) is a model-based glycaemic control protocol using a stochastic model to forecast distributions of likely future changes in insulin sensitivity (SI) based on its current value. This can be used to determine likely future blood glucose (BG) levels for a given intervention, enabling the most optimal dose selection that best overlaps a clinically defined BG target band. This study presents a novel 3D model capable to predict likely future distribution of SI using both current SI and its prior variability (%ΔSI). Methods: Metabolic data from 3 clinical ICU cohorts totalling 819 episodes and 68629 hours of treatment under STAR and SPRINT protocols are used in this study. Data triplets (%ΔSIn, SIn, SIn+1) are created and binned together in a range of %ΔSI = [-100%, 200%] and SIn = [1.0e-7, 2.1e-3] in bin sizes of %ΔSI = 10% and SIn = 0.5e-4. The 5th, 50th, and 95th percentile of SIn+1 are determined for each bin where data density is high enough (>100 triplets) and compared to the previous stochastic model. The predictive power of the two models are compared by computing median [IQR] per-patient percentage prediction of SI within the 5th-95th and 25th-75th percentile ranges of model predictions. Results: Results show the previous model is over-conservative for ~77% of the data, mainly where %ΔSI is within an absolute 25% change. The percentage change in the 90% CI width in this region is reduced by ~25-40%. Conversely, non-conservative regions are also identified, with 90% CI width increased up to ~80%. Predictive power is similar for both model (60.3% [47.8%, 71.5%] vs. 51.2 [42.9%, 59.2%] within 25th-75th and 93.6% [85.7%, 97.3%] vs. 90.7% [84.4%, 94.6%] within 5th-95th range). Conclusions: The new 3D model achieved similar predictive power as the previous model by reducing the 5th-95th percentile prediction range for 77% of the data, predominantly where SI is stable. If the conservatism of the previous model reduces risk of hypoglycaemia, it also inhibits the controller’s ability to reduce BG to the normal range by safely using more aggressive dosing. The 3D new model thus better characterises patient-specific response to insulin, and allows more optimal dosing, increasing performance and safety. [less ▲]

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See detailA multiscale model of the human cardiovascular system can account for the Frank-Starling effect on vascular filling therapy
Kosta, Sarah ULg; Pironet, Antoine; Negroni, Jorge et al

Poster (2017, February 01)

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See detailZinc, manganese and iron interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2017, February 01)

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See detailEvaluating the specificity of [18F] UCB-H for the isoform SV2A, compared with isoforms SV2B and SV2C
Serrano Navacerrada, Maria Elisa ULg; Becker, Guillaume ULg; Bahri, Mohamed Ali ULg et al

Poster (2017, February 01)

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important in normal and pathological process, like the ... [more ▼]

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important in normal and pathological process, like the epilepsy (1, 2). [18F]UCB-H was developed like a tool to study the role of this isoform with neuroimaging techniques (3, 4). The objective of this study was to evaluate its specificity to this isoform comparing with the others, through a competition assay in rats with ex-vivo autoradiography and mPET imaging. Methods: Forty male Sprague-Dawley were used in ex-vivo autoradiography experiments (N=20) and in microPET imaging (N=20). Animals were pre-treated 30 minutes before the injection of [18F]UCB-H with a dose IP either of vehicle, Keppra (SV2A ligand), UCB068 (SV2B ligand) or UCB054 (SV2C ligand). Ex-vivo autoradiography was carried out 5 minutes after radiotracer injection while mPET images were acquiring with a dynamic scanner of 1 hour. Data were expressed in Standard Uptake Value and then, the area under the curve was calculated for the total process. Results: In ex-vivo autoradiography, ANOVA of two-ways showed statistical significant differences in brain uptake of [18F]UCB-H among the groups pretreated with Keppra or the ligand for SV2B and the control group. Regarding mPET data, statistical significant differences were found between the group injected with keppra and the rest of groups. Conclusion: Even if a considerable affinity between the ligands UCB068 and UCB054, and the receptor for the isoform SV2A exists, it is only detected during the first 5 minutes (ex-vivo technique), being certainly due to a nonspecific binding. This binding is not strong enough to show a direct competition with the radiotracer during a mPET acquisition. These results allow us to conclude that [18F]UCB-H is a suitable radiotracer for the imaging of the isoform SV2A in vivo, allowing us the clinical study about the molecular base of a disease with a high population impact, like the epilepsy. [less ▲]

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See detailMegaloolithid dinosaur eggs: scrambled parataxonomy and nesting strategies
Jentgen, Benjamin ULg; Stein, Koen; Fischer, Valentin ULg

Poster (2017, February)

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See detailCharacterization of the low-mass companion HD 142527 B
Christiaens, Valentin ULg; Casassus, Simon; Absil, Olivier ULg et al

Poster (2017, February)

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See detailNew data on the Mesozoic radiation of chelonioids
Scavezzoni, Isaure ULg; Fischer, Valentin ULg

Poster (2017, February)

"Turtles" (Testudines) form a successful group of reptiles with several terrestrial, marine and fresh-water species. Their peculiar and somewhat constrained morphology (i. e. : carapace incorporating ribs ... [more ▼]

"Turtles" (Testudines) form a successful group of reptiles with several terrestrial, marine and fresh-water species. Their peculiar and somewhat constrained morphology (i. e. : carapace incorporating ribs, curved limbs, anapsid skull exempt of temporal fenestrae) and ecology has often obscured their relationships and, hence, their evolutionary history, notably in marine turtles (chelonioids). Modern chelonioids are divided in two clades (i. e. : shoft-shelled turtles and hard-shelled turtles) supported by distinct morphological and embryological characters. Their origin is traced back up to the Cretaceous, along with a series of extinct forms, many of which being collectively known as Protostegidae. Fossil evidence show that at least five clades of marine turtles were roaming the seas at the end of the Cretaceous. In fact, chelonioids appeared during the first stages of the Early Cretaceous and quickly exploded to reach a high level of disparity at the lowermost part of the late Cretaceous. Therefore, the Mesozoic radiation of chelonioids must have happened during the "middle" Cretaceous (especially the Aptian-Albian interval). However this radiation is poorly understood as the phylogenetic relationships of marine turtles are not resolved yet. Bringing new data may help resolve these issues, and it is the exact reason why the genus Rhinochelys is being investigated. [less ▲]

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See detailEcological intensification of fish production : Fertilization strategies in Africa fish farms
Mafwila Kinkela, Patrick ULg; Kambashi Mutiaka, Bienvenu ULg; Rollin, Xavier et al

Poster (2017, February)

Ponds fertilization has been found as strategies of intensification fish production for farm in Africa were commercial feeds are not available and ingredients for formulate completed feed are cost ... [more ▼]

Ponds fertilization has been found as strategies of intensification fish production for farm in Africa were commercial feeds are not available and ingredients for formulate completed feed are cost. However, farmers are not safe to any danger because the efficiency of these strategies is not always known owing to the associated risk of eutrophication. An experience was making to assess how various fertilization practices would impact fish growth and physicochemical parameters of water in the pond. [less ▲]

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See detailThe Bright and Dark Sides of elliptical galaxies
Biernaux, Judith ULg

Poster (2017, January 29)

Detailed reference viewed: 25 (4 ULg)