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See detailDisentangling soil from plant methanol exchanges in a maize field: a first step
Bachy, Aurélie ULiege; Mozaffar, Ahsan ULiege; Aubinet, Marc ULiege et al

Poster (2016, June)

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both ... [more ▼]

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both components). It bases on the following observation: methanol exchanges on bare soil (measured when maize was at germination stage) were as important as when plants were fast growing (and thereby, when the highest methanol plant methanol emissions were expected), and this under similar weather conditions. The goal of this poster is thus to understand why emissions were similar at these two periods. First, it addresses the question of the actual contribution of maize plants in methanol exchanges, by comparing up-scaled methanol exchanges measured on maize at leaf-scale (Mozaffar A.) to those measured at ecosystem-scale. Then, it investigates methanol exchanges mechanisms in order to evaluate how did soil methanol emissions evolve along the maize growing season. At the end of this poster, the hypothesis of decreasing soil methanol emissions along the maize growing season is proposed. [less ▲]

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See detailBeyond stuttering : Speech disfluencies in normally fluent, French-speaking children at age four
Leclercq, Anne-Lise ULiege; Suaire, Pauline; Moyse, Astrid

Poster (2016, June)

The aim of the present study is to establish normative data concerning the speech disfluencies existing in normally fluent children speaking French at age 4, an age at which stuttering has begun in 95% of ... [more ▼]

The aim of the present study is to establish normative data concerning the speech disfluencies existing in normally fluent children speaking French at age 4, an age at which stuttering has begun in 95% of children who stutter (Yairi & Ambrose, 2013). [less ▲]

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See detailSeasonal variation in human COGNITIVE brain responses
Meyer, Christelle; Muto, Vincenzo ULiege; Jaspar, Mathieu ULiege et al

Poster (2016, June)

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See detailCan serum progesterone be used to decide altrenogest supplementation discontinuation in problem pregnancies in mares?
Parrilla Hernandez, Sonia ULiege; Delahaut, Philippe; Ponthier, Jérôme ULiege et al

Poster (2016, June)

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation ... [more ▼]

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation. Timing when it can safely be stopped is frequently uncertain and therefore, supplementation is often continued until 100-120 days, when the placental secretion of progestagens has taken over. The aim of this study was to determine if altrenogest interfered with progesterone assay and if timing of altrenogest administration discontinuation could be based on the blood concentration of P4. Data were obtained from 7 healthy standard breed mares in seasonal anoestrus and in dioestrus. Mares were considered anoestrus when no follicles larger than 15 mm in diameter or corpus luteum (CL) were found at 2 consequent ultrasound examinations 7 days apart. They were confirmed dioestrus based on an observed ovulation and the presence of a CL 2 days after. Mares were treated with 0.044 mg/kg of altrenogest (Regumate®, Intervet, Boxmeer, Netherlands) orally once per day during 12 days beginning at any time for the anestrus group and at day 2 after ovulation for the dioestrus group. Blood was collected at day 0, 6, 12, 18 right before altrenogest administration and the serum was stored at -20°C until analysis. P4 concentration was obtained by radioimmunoassay and altrenogest by liquid chromatography-mass spectrometry. Differences in P4 and in altrenogest concentrations between different sampling days were determined by Friedman non parametric test. Concentrations of altrenogest before treatment were almost not detectable (negative control). At day 6, they were significantly higher (p<0.05), and were back to basal, almost not detectable values at day 18, in both groups. P4 concentration was basal with values < 0.4 ng/ml all through the experiment in the anoestrus group. In the dioestrus group, P4 concentration was variable from one mare to another, but it was significantly higher in mid-dioestrus (day 6) as compared to the time of the impending next oestrus (day12). No cross-reaction between P4 and altrenogest assays was observed as illustrated by the fact that concentration of P4 during anoestrus remained basal with or without supplementation. Despite the treatment, the variation of P4 over time was as expected in both groups. Altrenogest was no longer detectable 6 days after the last administration. Further studies with larger numbers and sampling in dioestrus to compare P4 levels in treated and untreated mares to investigate the potential effect of altrenogest on endogenous P4 production by the CL are needed. However, our experiment shows that P4 levels assayed by RIA can be trusted to evaluate if the secondary CL’s have become functional and can thus maintain the pregnancy while oral altrenogest supplementation is discontinued. [less ▲]

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See detailImportance de l’utilisation du Système d’Information Géographique pour les vastes sites de plein air du Paléolithique moyen en France septentrionale. L’Exemple de Caours (Somme, France) et Beauvais (Oise, France).
Moreau, Gwénaëlle ULiege; Locht, Jean-Luc

Poster (2016, June)

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement ... [more ▼]

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement, raising complication in the study of its dynamics at a regional scale. Therefore, some aspects of Neanderthals behaviour are still mischaracterize: in most cases, the function of the site and its territorial management over the northern France have to be specified. The spatial analysis of open air sites from northern France will help us to answer those questions. However, for Middle Palaeolithic sites, we can’t see systematically on the field the spatial organisation directly, we sometimes need modelization. Therefore, we started to build a spatial analysis protocol adapted to this site and applied to such sites. The sites of Caours (Somme, France) and Beauvais (Oise, France) are two open air sites that are exceptionally well preserved and displaying a large amount of faunal and lithic rests. Both are perfect candidate to apply and test the new protocol. First results proved that for each site the existence of a spatial organisation as remains concentration zones. Then, we characterize this areas – number, distribution. Finally, we were able to associate them to human activity areas like hearths, butchery or knapping areas. [less ▲]

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See detailAssessment of fine scale population genetic diversity and regeneration in Congo basin logged forests
Evrard, Quentin ULiege; Daïnou, Kasso ULiege; Hardy, Olivier et al

Poster (2016, June)

Background In the Congo Basin most of the light-demanding timber tree species display a deficit of natural regeneration which is a major handicap for sustainable production and certification. Whilst the ... [more ▼]

Background In the Congo Basin most of the light-demanding timber tree species display a deficit of natural regeneration which is a major handicap for sustainable production and certification. Whilst the majority of scientists investigate abiotic and biotic factors explaining that pattern, we hypothesize that tree population density or individual spatial isolation may also affect the tree fitness through inbreeding. In this study, we integrate ecological and genetic approaches to characterize the regeneration potential of a set of priority timber species by (i) estimating pollen dispersal distances at various tree population densities, and (ii) evaluating the impact of increasing spatial isolation on mating characteristics and tree fitness. The ultimate goal is the proposal of minimum population density that prevents inbreeding consequences. Method This ongoing study focuses on 10 timber species (Pericopsis elata, Milicia excelsa, Baillonella toxisperma, Entandrophragma cylindricum, E. utile, E. angolense, E. candollei, Afzelia bipindensis, Erythrophleum suaveloens, Terminalia superba). The data collection was carried out in the logging concession granted to Pallisco in Cameroon. We established two 400-ha plots, where all individuals (DBH > 10 cm) of the target species were inventoried and mapped. A sample of leave or cambium was collected for each of these individuals, as well as for seedlings to characterize patterns of gene flow using genetic tools (nuclear microsatellites). Dispersal agents were identified by direct observations and camera traps. Germination success was characterized in nursery for seeds collected on trees under an increasing isolation gradient. Results Main dispersal agents (wind, bat, rodent) and predators (rodent) were identified for all the species. The gene flow and germination data is still being analyzed and the main results will be presented in the poster. Conclusion Our data will allow characterizing the reproductive biology of a set of important timber species from the Congo basin. These information will strengthen sustainable forest management and the application of certification by adjusting harvesting norms through the use of scientifically-relevant data. In particular, we will tentatively define a maximum distance to be maintained between two adults to allow a qualitative reproduction. [less ▲]

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See detailChronology of in vitro maturation of donkey (Equus asinus) oocytes collected by ovum pick up
Douet, Cécile; Goudet, Ghylène; Kaabouba-Escourier, Aurore et al

Poster (2016, June)

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in ... [more ▼]

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in Italy, the Zamorano-Leonés donkey in Spain and the Normand donkey in France. Their preservation includes the creation of a Genome Resource Bank. Embryos cryopreservation allows the preservation of genetics from both male and female and is the fastest method to restore a breed. However, embryo production in vivo is limited in equids. In vitro production of embryos would allow the production of several embryos per cycle, but no technique has yet been developed in the donkey for in vitro production of embryos. In that context, ovum pick up (OPU) would come as a very helpful tool both for embryo production and research purposes. Our objective was to develop OPU in jennies and to analyze the chronology of in vitro maturation of donkey oocytes. Donkey cumulus-oocyte complexes (COCs) were collected by OPU from 5 to 25mm follicles in adult cyclic jennies. During OPU, jennies were injected detomidine, butorphanol, and butylscopolamine for sedation, analgesia and antispasmodia. COCs were in vitro matured in TCM199 supplemented with fetal calf serum and epidermal growth factor for 24, 30, 34 or 38 hours in groups of 10 to 30 in an atmosphere of 5% CO2 in air at 38.5°C. Five OPU sessions were performed, with 3 to 6 females per session. We collected a total of 92 COCs out of 193 follicles (48%) with an average of 4.2 COCs per jenny. The recovery rate per follicle increased along the OPU sessions: 34%, 43%, 52%, 47% and 56%, probably due to the increasing skill of the staff. Despite a very large experience with OPU in horses, all staff members required some time to adjust to the specificities of the jenny vs mare, in particular to the differences in holding the ovary. All COCs were expanded after over 24 hours in vitro maturation (IVM). At collection, jennies COCs contained a germinal vesicle. After 24 hours IVM, COCs contained either a germinal vesicle or condensed chromatin. Metaphase 1 oocytes were observed after 30 hours IVM and 45% were in metaphase 2 after 34 hours IVM. Half of the COCs were degenerating after 38 hours IVM. In conclusion, we established for the first time conditions for ovum pick up in jennies and the chronology of in vitro maturation of donkey oocytes. We showed that in vitro maturation of donkey oocytes can produce 44% of metaphase 2 oocytes after 34 hours in culture while most equine oocytes reach metaphase 2 after 30 hours in culture. [less ▲]

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See detailDesign and evaluation of a high-density energy storage route with CO2 re-use, water electrolysis and methanol synthesis
Léonard, Grégoire ULiege; Giulini, Davide; Villarreal-Singer, Diego

Poster (2016, June)

In the context of the Energy Transition, electricity storage ranging from seconds to seasons is needed to increase the integration of variable renewables sources. The power-tofuel process uses a liquid ... [more ▼]

In the context of the Energy Transition, electricity storage ranging from seconds to seasons is needed to increase the integration of variable renewables sources. The power-tofuel process uses a liquid energy vector with high energy density for long-term energy storage. In the present work, we simulate the power-to-methanol process in Aspen Plus. Then, we use heat integration to increase the conversion efficiency from 40.1 to 53.0 %, evidencing large improvement potential thanks to process integration. Further work includes experimental design and development of control strategies. [less ▲]

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See detailIntegrated microfluidic device for gold nanoparticles synthesis
Emonds-Alt, Gauthier ULiege; Malherbe, Cédric ULiege; Monbaliu, Jean-Christophe ULiege et al

Poster (2016, May 31)

In order to perform the analysis of organic pollutant in trace by Raman spectroscopy, it is necessary to develop SERS substrates, such as noble metal nanoparticles (NPs). The synthesis of gold or silver ... [more ▼]

In order to perform the analysis of organic pollutant in trace by Raman spectroscopy, it is necessary to develop SERS substrates, such as noble metal nanoparticles (NPs). The synthesis of gold or silver NPs is used for analytical applications. It is therefore important to precisely control the synthesis of these SERS substrates to achieve reproducible measurements. Microfluidics is a very attractive technology for the control of the synthesis parameters such as temperature, reaction time and mixing conditions which affect both the size and the morphology of NPs. The synthesis of gold or silver NPs in microreactor is a technique we are already developing in the laboratory. We develop a method for the continuous synthesis of gold and silver NPs in a segmented regime capillary microreactor. The residence time and temperature have a significant effect on the size of the NPs, enabling to prepare different sizes of NPs using the same microreactor and the same precursor solutions. [less ▲]

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See detailGC×GC-TOFMS for forensic blood VOC profiling
Dubois, Lena ULiege; Perrault, Katelynn ULiege; Stefanuto, Pierre-Hugues ULiege et al

Poster (2016, May 30)

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See detailGC×GC-HRTOFMS to better understand cloak-and-dagger activities of Madagascar Mantella frogs
Stefanuto, Pierre-Hugues ULiege; Brasseur, Catherine; Cecchini, Harisoa et al

Poster (2016, May 30)

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See detailPeak dispersion evaluation and optimization in GC×GC
Stefanuto, Pierre-Hugues ULiege; Dimandja, Jean-Marie; Focant, Jean-François ULiege

Poster (2016, May 30)

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See detailEstetrol, a natural SERM exhibiting combined estrogenic and anti-estrogenic properties on mammary gland and breast cancer
Gallez, Anne ULiege; Gérard, Céline ULiege; Blacher, Silvia ULiege et al

Poster (2016, May 30)

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would ... [more ▼]

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would provide useful advances for patient care. Estetrol (E4) is a liver friendly promising candidate for HRT. In preclinical and/or clinical studies, E4 has been effective against the main symptoms of menopause from a starting dose of 0.3 mg/kg/day. The aim of this study was to define the impact of E4 on mammary gland and breast cancer development. Our preclinical data show that E4 is less efficient than estradiol (E2) to induce mammary gland growth. Treatment with several concentrations of E4 has shown that E4 did not increase tumor development, when it is used at 0.3 mg/kg/day. However, at 3 mg/kg/day, E4 increased tumor growth similarly to E2 (0.08 mg/kg/day). E4 presents also some anti-estrogenic effects on mammary gland and antitumor activity on breast cancer by decreasing the strong proliferative effect of E2. While ERα is the predominant receptor mediating its effects, the dual weak-estrogenic/anti-estrogenic feature of E4 results from differential signaling pathways activation. Both nuclear and rapid extra-nuclear signaling pathways are necessary for a complete estrogenic effect of E4. However, the antitumor action of E4 is not due to a capacity to antagonize E2-induced nuclear activity. In conclusion, our results support that E4, if it is used in strictly controlled clinical applications, could have no or only limited impact on breast and breast cancer. [less ▲]

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See detailAtaxie optique: cas E.M.
DEMOULIN, Valentine ULiege; Departz, Marie-Pierre; MAQUET, Pierre ULiege

Poster (2016, May 28)

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See detailSubstrats cérébraux du contrôle proactif et réactif dans le vieillissement non pathologique
Manard, Marine ULiege; François, Sarah ULiege; Salmon, Eric ULiege et al

Poster (2016, May 27)

Les recherches sur le contrôle cognitif proposent l’apparition d’un déclin lié à l’âge dans les capacités de contrôle proactif (forme anticipatoire de contrôle comportemental) alors que le contrôle ... [more ▼]

Les recherches sur le contrôle cognitif proposent l’apparition d’un déclin lié à l’âge dans les capacités de contrôle proactif (forme anticipatoire de contrôle comportemental) alors que le contrôle réactif (forme réactionnelle à la détection d’un conflit) semble rester intact [1]. Ces deux formes de contrôle ont été associées à des aires cérébrales différentes [2], cependant peu d’études ont étudié les effets de l’âge sur les régions cérébrales sous-tendant chacun de ces deux types de contrôle. Pour ce faire, une version adaptée de la tâche de Stroop a été administrée à 16 jeunes (18-30 ans) et 16 adultes (60-75 ans) en IRM fonctionnelle. Cette version de la tâche variait la quantité d’items interférents, permettant la création de trois contextes (Mostly Congruent « MC », Mostly Incongruent « MI » et Neutre) favorisant respectivement la mise en place des deux types de contrôle (réactif = congruent / proactif = incongruent). Les analyses ont été réalisées avec SPM8 (p<.001 non-corrigé). Les résultats comportementaux révèlent un traitement plus rapide de l’interférence dans le contexte « MI » que « MC » pour les jeunes adultes uniquement. Au niveau cérébral, le traitement de l’interférence en MI semble associé à une diminution d’activité (surtout à droite) dans les régions frontales et temporales chez les volontaires âgés par rapport aux plus jeunes, alors qu’en MC, les âgés montrent une augmentation d’activité bilatérale au niveau frontal. Ainsi, les résultats comportementaux montrent que les adultes âgés semblent avoir plus de difficultés à implémenter un contrôle proactif (associé à une diminution d’activité) que les jeunes dans les régions sous-tendant la performance à cette tâche. Cependant, le recrutement supplémentaire de régions frontales observé lors du contexte MC postulé pour favoriser le contrôle réactif, suggère la présence de mécanismes de compensation. Pour conclure, le vieillissement semble affecter de manière différentielle l’activité des régions cérébrales sous-tendant les différents types de contrôle. [less ▲]

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See detailGestalt Reasoning Promotes Extension Errors.
Dumitru, Magdalena ULiege; Joergensen, Gitte

Poster (2016, May 27)

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See detailEpithelial Rab guanine nucleotide exchange factor 1 (Rabgef1) deficiency increases susceptibility to DSS-induced intestinal inflammation in mice
El Abbas, Sophie ULiege; Beguin, C; Mesnil, Claire ULiege et al

Poster (2016, May 27)

Rab guanine nucleotide exchange factor (GEF)1 (Rabgef1) is a guanine nucleotide exchange factor for the endocytic GTPase Rab5, and also exhibits E3 ubiquitin ligase activity in vitro. In vivo functions of ... [more ▼]

Rab guanine nucleotide exchange factor (GEF)1 (Rabgef1) is a guanine nucleotide exchange factor for the endocytic GTPase Rab5, and also exhibits E3 ubiquitin ligase activity in vitro. In vivo functions of Rabgef1 remain largely unknown, but Rabgef1 is critical for health, as globally Rabgef1-deficient mice exhibit perinatal mortality and those surviving to adulthood spontaneously develop severe skin inflammation. In the mouse gut, we found that Rabgef1 is highly expressed in intestinal epithelial cells (IECs). We performed conditional deletion of Rabgef1 using the cre-lox system to obtain mice lacking Rabgef1 specifically in IECs (Rabgef1IEC-KO). These mice did not develop spontaneous intestinal abnormalities but showed an increased susceptibility to inflammation in dextran sodium sulfate (DSS)-induced colitis model. Indeed, compared to littermate controls, mice lacking Rabgef1 in IECs exhibited shorter and highly inflamed colons and higher inflammatory scores in histopathological examination of colons, suggesting that Rabgef1 expression regulates IEC function and is critical in limiting DSS induced inflammation and damage. In addition, we also showed that mRNA expression of pro-inflammatory cytokines, such as Il1b and Tnfa, was upregulated in IECs isolated from Rabgef1IEC-KO mice compared to the ones isolated from littermate controls. Taken together, these results suggest that Rabgef1 acts as a regulator of intestinal homeostasis, and that dysregulated Rabgef1 expression could contribute to intestinal barrier dysfunction in inflammatory conditions of the gut. [less ▲]

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See detailSingle molecule force spectroscopy on helical foldamers
Devaux, Floriane ULiege; Li, Xuesong; Huc, Ivan et al

Poster (2016, May 27)

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See detailDESIGN OF HIGH-AFFINITY LIGANDS FOR THE BENZOTHIADIAZINE ALLOSTERIC BINDING SITE OF THE AMPA RECEPTORS
Drapier, Thomas ULiege; Geubelle, Pierre ULiege; Bouckaert, Charlotte et al

Poster (2016, May 26)

L-glutamic acid is the major excitatory neurotransmitter in the brain. It exerts its effects through metabotropic and ionotropic receptors. Among the latter, three subtypes have been identified: NMDA ... [more ▼]

L-glutamic acid is the major excitatory neurotransmitter in the brain. It exerts its effects through metabotropic and ionotropic receptors. Among the latter, three subtypes have been identified: NMDA, AMPA and KA receptors. It is now well established that a deficit in glutamatergic signaling may be responsible for neurological disorders such as mild cognitive impairment, schizophrenia, depression, and ADHD. Enhancement of the signal through positive allosteric modulators of AMPA receptors might be a therapeutic issue for these diseases. These compounds are expected to exert a fine tuning of the signal. Since they require the presence of the endogenous ligand to be active, they are expected to induce less toxicity than agonists. In this context, based on the structure of known allosteric modulators of AMPA receptors such as cyclothiazide and IDRA 21, the Laboratory of Medicinal Chemistry (University of Liège) has developed a series of 1,2,4-benzothiadiazine 1,1-dioxides with high potency as AMPA receptor potentiators. Crystallographic data obtained by the Department of Drug Design and Pharmacology (University of Copenhagen) highlighted those potentiators bind to two contiguous sites at the dimer interface of the ligand binding domain (LBD) of the AMPA receptor1,2. Based on these data, we may expect that the synthesis of dimeric molecules could lead to further improvement in affinity and activity. This assumption was reinforced by docking experiments conducted with virtual examples of dimeric compounds on the GluA2-LBD (collaboration with NAMEDIC). The present work is thus focusing on the preparation of a family of dimeric benzothiadiazine dioxides. Moreover, in collaboration with GIGA-Molecular Pharmacology, we are developing a pharmacological in vitro assay based on the measurement of Ca2+ inflow through a fluorimetric method. This medium-throughput screening will enable the characterization of our new compounds and the validation of our working hypothesis. [less ▲]

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See detailHéboïdophrenia: The hazardous interplay of psychosis and psychopathy
De Page, Louis; Englebert, Jérôme ULiege

Poster (2016, May 24)

Introduction The diagnostic entity of heboidophrenia has been consigned to oblivion despite its clinical, prognostic, criminogenic value. At the core of this diagnosis lies specifics interactions between ... [more ▼]

Introduction The diagnostic entity of heboidophrenia has been consigned to oblivion despite its clinical, prognostic, criminogenic value. At the core of this diagnosis lies specifics interactions between psychosis and psychopathy. The diagnosis was first coined by Kalhbaum in 1890, was revived by Anglo-Saxon literature in the first half of the 20th century, and by French psychiatrists in the late 90’. Method We integrated literary sources, clinical experience and an own pilot study as a stepping-stone for a more in-depth research. Cases (n=20) were selected out of samples of Mentally Disordered Offenders in Belgian Medium and High Risk Forensic Facilities. Files, records and therapist’s experiences were quantitatively analysed iteratively to cull subtypes from each other. We here present preliminary qualitative results, more indepth qualitative and quantitative results will be produced. Results The diagnosis subsumes more than a mere DSM-IV-TR Axis I & II comorbidity, it can be seen from a developmental perspective, or from a structural perspective. Both vantage points suggest an interplay between the (paranoid) psychotic process and the antisocial behaviour, where both trigger and induce each other. At this point, we found evidence for three subtypes modes of interplay; 1) mood instability patterns induced either psychopathic or psychotic phenotypic presentation, 2) according to personality organization and its defensive functioning (were psychopathy defends against psychosis), and 3) phenotypic expression of both components (psychopathy and psychosis) linearly co-dependent. Discussion Despite its incremental value, little is known about the therapeutic prospects of this diagnosis. Current results indicate that these patients do question competency to answer for their deeds in a peculiar manner, relapse more often, reoffend more often, do evade treatment opportunities (if they have any), act out more often (in both antisocial and psychotic ways), induce a particular negative counter-transference in psychiatric teams, etc. Because of these particularities and their versatile phenotype presentation, heboidophrenia patients cannot simply be assimilated to the “high risk & high care” population, but need special supervision and clinical insight. In this research, we aim(ed) at discerning semiologic signs in order to support the diagnostic process. [less ▲]

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See detailAbnormal bodily experiences in schizophrenia: a phenomenological study
lancellotti, Elisa; Englebert, Jérôme ULiege

Poster (2016, May 24)

Our study focuses on Abnormal Bodily Experiences [ABE] in people with schizophrenia. We have created a specific questionnaire for this study – based on the study of Stanghellini et al. (2012) and the ... [more ▼]

Our study focuses on Abnormal Bodily Experiences [ABE] in people with schizophrenia. We have created a specific questionnaire for this study – based on the study of Stanghellini et al. (2012) and the third dimension of the EASE scale (body experiences) (Parnas et al. 2005). Our research follows an analytical method called “empirical-phenomenological”. Our population is made up of 50 schizophrenic subjects. The average age of subjects was 37 years. There are no exclusion criteria regarding gender or control sample. Analyzes are qualitative. We observed that ABE are very present symptoms in people with schizophrenia. Indeed, in our sample, only one patient was asymptomatic. We also observed that all categories and subcategories were present in our sample. These results should help to refine and clarify the diagnosis of schizophrenia and open up significant opportunities for psychotherapeutic dimension. [less ▲]

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See detailA phenomenological approach of anorexia nervosa
Minguet, Eugénie; Englebert, Jérôme ULiege; Helinski, Adam et al

Poster (2016, May 24)

Introduction: from a phenomenological perspective, anorexic female patients seem to experience difficulties regarding the subjective feeling of their own body (embodiment) and their personal identity ... [more ▼]

Introduction: from a phenomenological perspective, anorexic female patients seem to experience difficulties regarding the subjective feeling of their own body (embodiment) and their personal identity, which lead to an intersubjectivity impairment within social interactions. Following these findings, this study investigates the “body-for-others” concept (Sartre, 1943) and altruistic hypotheses from the ethologist Albert Demaret (1973) in a sample of anorexic female patients. Two main hypotheses are tested: anorexic patients are expected to define themselves mostly through the gaze of others and as more altruistic than control participants. Method: this study included 67 anorexic female patients (based on the DSM-IV criteria) and 246 control female participants. Three self-reported questionnaires were used: the Eating Disorder Inventory: EDI-II of Garner (French version), the Identity and Eating Disorders questionnaire: IDEA of Stanghellini (French version translated and adapted by us) to assess the “body-for-others” concept and 17 complementary items for the altruistic dimension. Mann-Whitney non-parametric tests were used to compare the two groups. Results: results show that anorexic patients obtain higher scores than the control group for the three questionnaires (p<0,001 for all measurements). Discussion: the IDEA questionnaire enriches the knowledge relative to the body-self relationship and the first-person perspective, which is essential for a phenomenological approach. Furthermore, the promising results concerning the altruistic dimension in anorexia nervosa should be evaluated in future research. Nevertheless, a better validity of the items assessing the altruistic dimension is needed. [less ▲]

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See detailModelling of the β-lactamase BlaP induction in Bacillus licheniformis
Dauvin, Marjorie ULiege; Raymackers, Alice ULiege; Delvigne, Frank ULiege et al

Poster (2016, May 24)

In bacteria, the production of a β-lactamase, an hydrolase specific to β-lactam antibiotics, may be constitutive or inducible. In Bacillus licheniformis 749/I the presence of a β-lactam in the external ... [more ▼]

In bacteria, the production of a β-lactamase, an hydrolase specific to β-lactam antibiotics, may be constitutive or inducible. In Bacillus licheniformis 749/I the presence of a β-lactam in the external media is detected by a protein relay producing an intracellular signal which leads to the induction of BlaP β-lactamase expression. The blaP gene is included in a divergeon along with blaI, coding for a cytoplasmic repressor, and blaR1, coding for a penicillin membrane receptor. Both, the acylation of the extracellular domain of BlaR1 by a β-lactam together with cellular stress due to the presence of the antibiotic outside the cell generate a dipeptide (coactivator) resulting from the peptidoglycan turnover that destabilizes BlaI repressor-DNA complex, leading to the expression of β-lactam resistance. [less ▲]

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See detailWould you care for some integrated care in your fragmented health system? A participatory action research to improve integration between levels of care in a Belgian urban setting.
Belche, Jean ULiege; Duchesnes, Christiane ULiege; Darras, Christian et al

Poster (2016, May 24)

Integration between levels of care is not facilitated by the Belgian health system. Indeed, patients have uninhibited access to every level of care, there is no gatekeeping system, and no structural ... [more ▼]

Integration between levels of care is not facilitated by the Belgian health system. Indeed, patients have uninhibited access to every level of care, there is no gatekeeping system, and no structural coordination between levels of care. Meanwhile, on one hand, the occurrence of more complex care situations in the ambulatory setting is enhancing the need for coordination while on the other hand, hospitals face financial constraints to provide care in the community. The aim of the research was to organize coordination between levels of care at the local level, in an urban setting. We used the “Local Health System” model (LHS) that aims at integrating hospital and primary care activities for a defined population at the local level. We chose the participatory action research (PAR) methodology and its spiral plan-do-study-act cycles, to ensure the participation and implementation of results by the targeted actors (hospital and local general practitioners (GP) organizations). [less ▲]

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See detailLife cycle assessment of hemp concret blocks
Groslambert, Sylvie ULiege; de Mahieu, Jean-Baptiste; Léonard, Angélique ULiege

Poster (2016, May 24)

Buildings notably contribute to global environmental negative impacts due to consumption of both embodied energy and natural resources as well as various emissions during their whole life cycle. It is ... [more ▼]

Buildings notably contribute to global environmental negative impacts due to consumption of both embodied energy and natural resources as well as various emissions during their whole life cycle. It is therefore necessary to develop practices to reduce these impacts, mainly by reducing the part of non-renewable resource in material as well as by ensuring the lowest energy consumption possible during their lifetime. New developments in natural fibres and their use in insulating materials can lead to significant improvement in building environmental impact. For this purpose, assessment of environmental performance is needed to support both the design and the production of (new) fibre based insulation solutions. In this context, the Life Cycle in Practice (LCiP) project helps SMEs to reduce the environmental impacts of their products and services across the entire life cycle. Within the frame of this project, Isohemp (BE) hemp concrete block impact is evaluated in a cradle-to-gate LCA. Functional unit is a pallet of hemp concrete blocks ready for shipping. It represents about 1.3 m³ of blocks. Hemp blocks are made by pressing a mix of hemp shives, hydraulic and hydrated limes, and water. Long term carbon storage due to lime carbonation induces a large benefit in Climate Change (CC) category. The balance of the CC indicator for hemp cultivation is also favorable due to carbon dioxide uptake by the photosynthesis occurring during the plant growth. Life Cycle Assessment of hemp concrete blocks ready to ship has also highlights some improvements that can easily be made at the packaging level in order to lower the global environmental impact and increase the sustainability of this insulation material. Data are processed in SimaPro 8 software, with Ecoinvent 3 and ELCD 3 databases, and analyzed with the CML IA method. This method is compliant with the indicators required by EN15804 standard in order to communicate on the environmental performance of Isohemp blocks. [less ▲]

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See detailPeptidoglycan fragments separation by CE/LC-MS
Boulanger, Madeleine ULiege; Delvaux, Cédric ULiege; Far, Johann ULiege et al

Poster (2016, May 24)

In Bacillus licheniformis 749/I, the induction of BlaP beta-lactamase relies on a complex regulation system. During this process, the intracellular repressor BlaI is inactivated by a dipeptide coming from ... [more ▼]

In Bacillus licheniformis 749/I, the induction of BlaP beta-lactamase relies on a complex regulation system. During this process, the intracellular repressor BlaI is inactivated by a dipeptide coming from the peptidoglycan (PG) degradation via an “AND Gate” regulation. This regulation involves the cellular stress induced by the beta-lactam, the membrane receptor BlaR1 and the PG turnover. Briefly, the induction occurs when the extracellular domain of BlaR1 is acylated by the antibiotic which leads to a reorganization of the transmembrane segments and the receptor autocleavage. Simultaneously, the beta-lactam partially inhibits the penicillin-binding protein 1 (PBP1), triggering increased PG turnover and accumulation of PG fragments. Some of these fragments could enter in the cytoplasm and undergo enzymatic degradations which lead to the formation of the pro co-activator (tripeptide L-Ala-D-Glu-m-A2pm). This pro co-activator generates the co-activator, the dipeptide D-Glu-m-A2pm. Nowadays the nature and the concentration of PG fragments inside the cytoplasm are unknown. Therefore, the development of different analytical methods is required in order to identify those cytoplasmic fragments. In this poster, three different ways to separate PG fragments are discussed. [less ▲]

Detailed reference viewed: 89 (9 ULiège)
Peer Reviewed
See detailIntimate partner violence: Perseverance vs Dropout from treatment programs targeting male batterers
Di Piazza, Laetitia ULiege; Kowal, Cécile; Hodiaumont, Fabienne et al

Poster (2016, May 24)

Introduction: The Dropout phenomenon has been studied extensively in general psychotherapy and in more specific domains. Therapeutic programs for male batterers across Europe, United States or Canada also ... [more ▼]

Introduction: The Dropout phenomenon has been studied extensively in general psychotherapy and in more specific domains. Therapeutic programs for male batterers across Europe, United States or Canada also have their share of problems in their attempts to maintain users in treatment. The purpose of this study is to identify potential intrapsychic variables associated with dropout and completion of this kind of treatment. Method: Fifty one male offenders enrolled in a group treatment for domestic violence took part in the study (23 who abandoned and 28 who completed). Before the start of therapy, they were surveyed using questionnaires and structured clinical interview to collect sociodemographic data and to assess specific psychological variables, namely emotional distress (BDI), impulsive behavior (BIS-11), early relationship with their parents (PBI), life events (LEDS) and alexithymia (TAS-20), the inability to experience and express subjective emotions. Results: Correlational analysis showed that age, paternal parenting behaviors and the number of significant events reported in the past six months are the only variables correlated with treatment dropout. The younger the participants, the most likely they were to dropout of the group therapy. Moreover, men who completed treatment reported fewer difficulties in their life and less autonomy support by their father than men who prematurely terminated their therapy. Discussion: The present findings pose a serious challenge for all actors involved in specifics treatment programs. Indeed, if all individuals who committed violent acts have the same intrapsychic variables, how do we predict, especially retain and motivate, the users likely to dropout? [less ▲]

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See detailMaternal sense of competence in a context of precariousness
Halin, Stéphanie ULiege; Glowacz, Fabienne ULiege

Poster (2016, May 24)

The exercise of parenthood is threatened by the precarious context in which parents and children live. Precariousness is defined by the lack or the absence of basic securities, such as housing, employment ... [more ▼]

The exercise of parenthood is threatened by the precarious context in which parents and children live. Precariousness is defined by the lack or the absence of basic securities, such as housing, employment, financial, emotional security, etc. In such a context, the emotional availability of a parent can be reduced, so that the quality of parent-child interactions decreases and multiple dimensions of the child development are impacted. Therefore, policies of prevention and intervention have been developed in order to overcome the difficulties experienced by parents from birth and, sometimes, from pregnancy. However, little is known about the parenting sense of competence of mother living in such a precarious context, and the possible mediating effect of perceived social support. In this study, we investigate parenting sense of competence in a sample of 38 adult mothers raising alone their children in a context of poverty. Subjects completed the French versions of the “Parenting Sense of Competence Scale (P.S.O.C.)” and the “Social Provisions Scale”, and a socio-demographic questionnaire. Results indicate that, in average, mothers report a high parenting sense of competence (μ=69.18), characterized by a high level of self-efficacy (μ=32.76) and an average level of satisfaction (μ=36.42). Mothers also report low perceived social support (T=41, percentile 21). Results also indicate a significant negative correlate between self-efficacy and the need to feel useful and necessary, while the satisfaction dimension is positively correlated with all the perceived social support dimensions, except from the “need to feel useful” dimension. In conclusion, mothers raising alone their children in a context of poverty report high levels of parenting sense of competence. However, withdrawal and isolation are associated with poorer parental satisfaction. This suggests that the social dimension of poverty is a significant aspect to consider in parenting support policies. [less ▲]

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See detailIncarceration and parenting : is parenting a desistance factor?
Puglia, Rosa ULiege; Servaes, Anne; Halin, Stéphanie ULiege et al

Poster (2016, May 24)

Incarceration leads to a break between an individual and one’s social environment, inducing significant losses of identity (Touraut, 2014). Tough delinquent identity remains prominent, during their ... [more ▼]

Incarceration leads to a break between an individual and one’s social environment, inducing significant losses of identity (Touraut, 2014). Tough delinquent identity remains prominent, during their incarceration, some inmates invest their role of father. Investing his role of father and thus his father’s identity allows the inmate to break away with delinquent’s identity (Tripp, 2009; Walker, 2009). Moreover, research on desistance underlines that turning point like job, marriage or parenthood supports the transition from criminal life to conventional life (Laub et Sampson, 2003; Lebel, Burnett, Maruna & Bushway, 2008). Indeed, becoming parent is a stage in life cycle that can shape one’s lifestyle. From qualitative researches conducted with father inmates since at least 6 months, we have identified the link between investment in parenting and desistance. The hypothesis is that investment in parenting has a positive impact on father’s prison experience, parenting sense of competence and it will support desistance process. A semi-structured interview and several questionnaires assessing relational and social life have been used in this context (WHOQOLbrief, EPS, ESUL, educational attitudes, QAECEP). First results highlight that parenting during incarceration can be difficult. Some inmates try to keep it with visits between inmate fathers and their children. These visits are influenced by different factors and have a positive impact on prison experience. Indeed, with these visits, inmates maintain a link with their children. That is a source of support and hope during incarceration. That allows investment in one father’s identity. [less ▲]

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See detailFe2O3 nanoparticle-functionalized N-doped carbon with interconnected, hierarchical porous structures as high-performance electrode for lithium ion batteries
Alkarmo, Walid ULiege; Ouhib, Farid ULiege; Aqil, Abdelhafid ULiege et al

Poster (2016, May 23)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−O2 batteries, Li−S batteries, supercapacitors, and fuel cells. A hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. Integrated as a lithium ion battery anode, NDC@Fe2O3 exhibited high reversible capacity of 930 mA h/g over 200 cycles. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

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See detailSynthesis of novel poly(vinylamine)-based copolymers with controlled compositions
Dréan; Guégan, Philippe; Detrembleur, Christophe ULiege et al

Poster (2016, May 23)

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful ... [more ▼]

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful in many fields such as coatings , water purification and gas membrane separation. The properties of such polymers strongly depend on the nature of the amines and can thus be adjusted by incorporating different types of amines along the backbone. In this perspective, we developed a strategy for synthesizing poly(vinylamine)-based (co)polymers containing primary and secondary amines as well as imidazole moieties in predictable proportions. First, radical (co)polymerizations of N-vinylacetamide, N-methylvinylacetamide and vinylimidazole were performed followed by deprotection of the amine functions via acidic hydrolysis of the pendant amides. We determined the reactivity ratios of each comonomer pairs and developed the corresponding Skeist’s model, allowing the prediction of the copolymer compositions and distributions. Following this straightforward approach, novel amine-containing copolymers with predictable and precise compositions were made available and should contribute in the future to the development of the above-mentioned applications. [less ▲]

Detailed reference viewed: 45 (3 ULiège)
See detailPolyphosphoester containing amphiphilic block copolymers as drug nanocarriers
Yilmaz-Ergül, Zeynep ULiege; Vanslambrouck, Stéphanie; Thiry, Justine ULiege et al

Poster (2016, May 23)

The design of drug delivery systems (DDS) often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, poly(ethylene oxide)-b ... [more ▼]

The design of drug delivery systems (DDS) often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, poly(ethylene oxide)-b-polyphosphoester amphiphilic block copolymers are known to self-assemble into polymer micelles when placed in water are appropriate drug carriers. In this work, an unsaturated alkene side-chain was introduced on the cyclic phosphate monomer according to a one-step reaction followed by its organocatalyzed polymerization initiated by a poly(ethylene oxide) macroinitiator. After self-assembly into water, the micelles were cross-linked by UV irradiation. Then, these cross-linked micelles have been loaded by doxorubicin, which is a drug to use in cancer therapy. We observed that the doxorubicin loading increased with the number of double bonds on the polyphosphate block of non-cross-linked micelles. This diblock amphiphilic copolymer bearing pendant unsaturations appears thus particularly promising candidate to build micellar drug delivery systems for intravenous injection. [less ▲]

Detailed reference viewed: 66 (5 ULiège)
See detailSynthesis of CO2-sourced hydrogels by using the non-isocyanate polyurethane (NIPU) chemistry
Gennen, Sandro ULiege; Grignard, Bruno ULiege; Thomassin, Jean-Michel ULiege et al

Poster (2016, May 23)

Polyurethane (PUs) is one of the most important polymers and finds applications as elastomers, coatings, adhesives and sealants for automotive or construction. PU is also a material of choice in the ... [more ▼]

Polyurethane (PUs) is one of the most important polymers and finds applications as elastomers, coatings, adhesives and sealants for automotive or construction. PU is also a material of choice in the biomedical domain due to its good biocompatibility, biodegradation and mechanical properties. Especially, PUs hydrogels have been developed in the last years for biomedical applications such as soft contact lenses, wound dressing, drug delivery systems and scaffolds for tissue engineering. Traditionally, PUs are synthesized by a step-growth polymerization between diols and diisocyanates. Because of toxicity issues and a possible interdiction of isocyanates, we focused on developing new PU hydrogels using a non-isocyanate route (Figure 1). The polyurethanes formed by this route are called NIPU (for Non-Isocyanate PolyUrethane). Firstly, chemically cross-linked NIPU gels were synthesized by solvent-free polycondensation between a hydrophilic CO2-sourced polyethyleneglycol bi-cyclic carbonate and a diamine in the presence of a crosslinker. Then, NIPU gels were swelled in water till water equilibrium before characterization of their mechanical properties by compression tests. The influence of the cross-linking ratios (diamine/crosslinker ratio) and diamine structure on the swelling and the compression properties were studied. To reinforce the compression properties of NIPU hydrogel (increase in stress at break, strain at break and compression modulus), a nanofiller was dispersed in the cyclic carbonate/diamine/crosslinker formulation prior to polymerization. For the first time, nanocomposite NIPU hydrogels with high water contents (up to 80%) and good compression properties have been prepared by using low clay content. [less ▲]

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See detailTuning morphology and surface properties of emulsion-templated porous polymers by controlled radical polymerization
Mathieu, Kevin ULiege; Jérôme, Christine ULiege; Debuigne, Antoine ULiege

Poster (2016, May 23)

Macroporous polymer monoliths with interconnected structures have attracted considerable interests in the last decades and sustain many applications in the fields of supported catalysis, chromatography ... [more ▼]

Macroporous polymer monoliths with interconnected structures have attracted considerable interests in the last decades and sustain many applications in the fields of supported catalysis, chromatography, microfluidic, water purification, membrane separation, tissue engineering, to name but a few. A very popular and straightforward synthetic approach for such open-cell polymers is the High Internal Phase Emulsion (HIPE) polymerization method. The latter consists in three steps: (i) stabilization of an emulsion in which an internal phase with a volume fraction higher than 74% is dispersed within a minor continuous phase containing a polymer network precursor, (ii) curing of the polymer phase by polymerization or crosslinking reactions, (iii) removal of the internal phase to release the porosity. The surface properties and morphology of the polyHIPEs are important features that must be controlled and adjusted for each applications. In this context, we prepared by controlled radical polymerization a series of well-defined amphiphilic copolymers having different hydrophilic-lipophilic balance (HLB) and chain-ends, used it as stabilizers for the emulsion-templated polymerizations and demonstrated the crucial effect of the structure of the macromolecular surfactant on the morphology of the polyHIPEs. Finally, the physical and chemical anchoring of the macromolecular surfactants at the surface pores were also considered for tuning the surface properties of the porous monoliths. [less ▲]

Detailed reference viewed: 41 (3 ULiège)
See detailSynthesis of novel well-defined poly(vinylamine)-based copolymers by controlled polymerization
Dréan, Mathilde; Guégan, Philippe; Detrembleur, Christophe ULiege et al

Poster (2016, May 23)

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful ... [more ▼]

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful in many fields such as coatings , water purification and gas membrane separation. The properties of such polymers strongly depend on the nature of the amines and can thus be adjusted by incorporating different types of amines along the backbone. In this perspective, we developed a strategy for synthesizing poly(vinylamine)-based (co)polymers containing primary and secondary amines as well as imidazole moieties in predictable proportions. First, radical (co)polymerizations of N-vinylacetamide, N-methylvinylacetamide and vinylimidazole were performed followed by deprotection of the amine functions via acidic hydrolysis of the pendant amides. We determined the reactivity ratios of each comonomer pairs and developed the corresponding Skeist’s model, allowing the prediction of the copolymer compositions and distributions. Following this straightforward approach, novel amine-containing copolymers with predictable and precise compositions were made available and should contribute in the future to the development of the above-mentioned applications. [less ▲]

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See detailA novel unsupervised clustering approach with multiple data types to reveal fine-level structure
Chaichoompu, Kridsadakorn ULiege; Tongsima, Sissades; Shaw, Philip James et al

Poster (2016, May 21)

Introduction: Several methods exist to identify population substructure that is due to shared genetic ancestry or regional proximity. These may be SNP-based or haplotype-based (Price et al. 2006, Lawson ... [more ▼]

Introduction: Several methods exist to identify population substructure that is due to shared genetic ancestry or regional proximity. These may be SNP-based or haplotype-based (Price et al. 2006, Lawson et al. 2012). Here, we present a flexible unsupervised clustering approach that is built on the ipPCA machinery (Intarapanich et al. 2009). Methods: Our method supports both numeric and categorical data, and can be applied to panels of SNPs and/or haplotypes, or gene-based integrative summaries (Fouladi et al. 2015). Unlike ipPCA, our method involves an iterative process using binary and ternary splits based on multivariate Gaussian mixture modeling of PCs and Clustering EM (CEM) estimation as in (Lebret et al. 2015). To assess performance, we considered different simulated scenarios of FST=[0.0005,0.006], 5,000-20,000 independent SNPs in HWE, 500-8,000 individuals, and 2-4 populations (Balding and Nichols 1995), with 100 replicates for each scenario. SNPs were treated as categorical or continuous (including ancestry-corrected SNPs). Haplotype-based runs used HapMap 3 data: CHB-JPT (FST=0.007) and CEU-TSI (FST=0.004). Result and Conclusion: In simulated scenarios of extremely subtle structure (FST=[0.0009,0.002]), a population classification accuracy of 92.56% or greater was obtained, which was superior to ipPCA. Promising results to detect fine structure were also obtained in case of the HapMap populations. We believe that the ability of our approach to detect subtle structure, including outlier individuals, will be important in molecular reclassification studies of patients from whom underlying population patterns have been removed. Grants: KC and KVS acknowledge FNRS, AS acknowledges ANR, ST acknowledges NSTDA, and PJS acknowledges TRF. [less ▲]

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See detailStudy of microbial phenotypic heterogeneity under bioprocess conditions using « single-cell » techniques
Delepierre, Anissa ULiege; Brognaux, Alison ULiege; Baert, Jonathan ULiege et al

Poster (2016, May 20)

Presentation of single-cell techniques in order to characterize microbial phenotypic heterogeneity: fluorescent labelling, biosensors, automated flow cytometry combined to proteomics approach.

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See detailStudy of the antioxidant action of morphine on the peroxidase cycle of MPO and HRP
Hoebeke, Maryse ULiege; Nyssen, Pauline ULiege; Franck, Thierry ULiege et al

Poster (2016, May 20)

Besides its analgesic action, morphine presents antioxidants properties and therefore an interest as inhibitor of MPO. Moreover, it has been proved that morphine can inhibit the ROS production and the PMN ... [more ▼]

Besides its analgesic action, morphine presents antioxidants properties and therefore an interest as inhibitor of MPO. Moreover, it has been proved that morphine can inhibit the ROS production and the PMN degranulation. Therefore, it seems like morphine has a direct action on MPO activities but the exact mechanism of action is still to be elucidated. The aim of the study is to investigate the potential antioxidant activity of morphine on the peroxidation cycle of MPO, in comparison to another peroxidase, Horseradish Peroxidase (HRP). [less ▲]

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See detailHuman in the forest: Long-term interactions, lifestyle and ecosystem services in Central Africa
Gillet, Pauline ULiege; Lhoest, Simon ULiege; Morin-Rivat, Julie et al

Poster (2016, May 20)

Detailed reference viewed: 39 (13 ULiège)
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See detailCharacterization of the impact of tillage on the root development and distribution of crop residues of a winter wheat crop by the use of NIR hyperspectral imaging
Fraipont, Guillaume ULiege; Eylenbosch, Damien ULiege; Fernández Pierna, Juan Antonio et al

Poster (2016, May 20)

The objectives of the study were to show the impact of tillage and crop residues management on the distribution of crop residues and root system of a winter wheat crop in the 0-30 cm soil profile.This ... [more ▼]

The objectives of the study were to show the impact of tillage and crop residues management on the distribution of crop residues and root system of a winter wheat crop in the 0-30 cm soil profile.This poster shows the results obtained during one crop year (2011-2012). This study is allowed by a rapid and innovative method of root system quantification based on the near infrared hyperspectral Imaging. [less ▲]

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See detailRole of Varicella Zoster virus ORF9p in the secondary egress : importance of its interaction with the cellular Adaptin Protein-1.
Lebrun, Marielle ULiege; riva, laura; lambert, julien et al

Poster (2016, May 20)

Detailed reference viewed: 52 (8 ULiège)
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See detailFarms For Future: building tomorrow's agriculture
Pécheur, Emilie ULiege; Maebe, Laura ULiege; Boeraeve, Fanny ULiege et al

Poster (2016, May 20)

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See detailExpérimentation d’un outil internet destiné à analyser la place accordée à l'activité physique au sein des écoles primaires
Cloes, Marc ULiege; Dell'Amico, Vincent; Delfosse, Catherine ULiege et al

Poster (2016, May 19)

L’école représente un des piliers essentiels de la promotion de l’activité physique mais il apparaît que nombre d’établissements ne sont pas conscients des actions qu’ils pourraient aisément mettre en ... [more ▼]

L’école représente un des piliers essentiels de la promotion de l’activité physique mais il apparaît que nombre d’établissements ne sont pas conscients des actions qu’ils pourraient aisément mettre en place dans cette perspective. Nous avons conçu un site internet présentant notamment des exemples d’initiatives de promotion d’activité physique et des recommandations pour la santé. Huit écoles primaires en ont reçu une copie et, pendant une période de 5 semaines, ont été invitées à y publier un minimum de 12 initiatives propres. Des contacts réguliers ont été établis entre les chercheurs et les praticiens. L’objet de cette recherche consiste à analyser l’exploitation du site par ces derniers. A la fin de l’étude, 4 écoles déclarent vouloir continuer à exploiter les données du site l’année suivante et 4 ont l’intention de s’inspirer des actions échangées à l’issue de l’expérience. Sept établissements estiment qu’il serait judicieux d’utiliser leur site comme un moyen de propagande. [less ▲]

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See detailImproving pose estimation by building dedicated datasets and using orientation
Azrour, Samir ULiege; Pierard, Sébastien ULiege; Van Droogenbroeck, Marc ULiege

Poster (2016, May 19)

Markerless pose estimation systems are useful for various applications including human- computer interaction, activity recognition, security, gait analysis, and computer-assisted medical interventions ... [more ▼]

Markerless pose estimation systems are useful for various applications including human- computer interaction, activity recognition, security, gait analysis, and computer-assisted medical interventions. They have attracted much interest since the release of low-cost depth cameras such as Microsoft’s Kinect camera. Shotton et al. and Girshick et al. pioneered tractable methods that infer a full-body pose reconstruction in real-time. Despite this technological breakthrough, the accuracy of human pose estimation from single depth images remains insufficient for some applications. Our work aims at building a simulation environment to create images databases suited for any camera position and improving the mainstream machine learning-based pose estimation algorithms. [less ▲]

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Peer Reviewed
See detailThe effect of one year of second-language immersion school program on cognitive development
Gillet, Sophie ULiege; Barbu, Cristina ULiege; Nkiani, Bwetol et al

Poster (2016, May 19)

Detailed reference viewed: 82 (7 ULiège)
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See detailChaotic Bohmian trajectories for stationary states
Cesa, Alexandre ULiege; Martin, John ULiege; Struyve, Ward

Poster (2016, May 18)

We study the possibility of chaos for the Bohmian dynamics when the wave function is stationary. Examples of stationary wave functions are given for which there is chaos, as demonstrated by numerical ... [more ▼]

We study the possibility of chaos for the Bohmian dynamics when the wave function is stationary. Examples of stationary wave functions are given for which there is chaos, as demonstrated by numerical computations, for one particle moving in 3 spatial dimensions and for two and three entangled particles in 2 dimensions. What is important for the amount of chaos is the overall complexity of the wave function. Some simple measures that partly capture the complexity of the wave function are considered: the participation ratio and different measures of entanglement. We find that these measures often tend to correlate to the amount of chaos. However, the correlation is not perfect, because the measures do not depend on the intrinsic complexity of the states of a given basis. [less ▲]

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See detailAharonov-Bohm oscillations of bosonic matter-wave beams in the presence of disorder and interaction
Chrétien, Renaud ULiege; Dujardin, Julien; Petitjean, Cyril ULiege et al

Poster (2016, May 18)

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that ... [more ▼]

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that is outcoupled from a magnetic trap into a 1D waveguide which is made of two semi-infinite leads that join a ring geometry exposed to a synthetic magnetic flux φ. We specifically investigate the effects both of a disorder potential and of a small atom-atom contact interaction strength on the AB oscillations. The main numerical tools that we use for this purpose are a mean-field Gross-Pitaevskii (GP) description and the truncated Wigner (tW) method. We find that a correlated disorder suppress the AB oscillations leaving thereby place to weaker amplitude, half period oscillations on transmission, namely the Aronov-Al’tshuler-Spivak (AAS) oscillations. The competition between disorder and interaction leads to a flip of the transmission at the AB flux φ = π. This flip could be a possible preliminary signature of an inversion of the coherent backscattering (CBS) peak. Our study paves the way to an analytical description of the inversion of that peak. [less ▲]

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See detailL'ordinateur dans la littérature allemande au tournant du millénaire
Dupont, Bruno ULiege

Poster (2016, May 18)

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See detailDevelopment of a hydrogen maser in the TE111 mode
Van Der Beken, Emeline ULiege; Léonard, Daniel; Counet, Arnaud et al

Poster (2016, May 18)

We present the recent progress in the development of a hydrogen maser in the unusual TE111 mode. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design ... [more ▼]

We present the recent progress in the development of a hydrogen maser in the unusual TE111 mode. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design hydrogen masers with significant reduced dimensions which represents a huge benefit for space applications and in particular for the global positioning system. We present in details the different parts of our model : cavity with a thin Teflon sheet, magnetic shielding, temperature control of the maser... [less ▲]

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See detailSuper- and subradiance from indistinguishable atoms with quantized motion
Damanet, François ULiege; Braun, Daniel; Martin, John ULiege

Poster (2016, May 18)

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See detailAnticoherence of spin states with point-group symmetries
Baguette, Dorian ULiege; Damanet, François ULiege; Giraud, Olivier et al

Poster (2016, May 18)

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See detailCharacterization and biocontrol properties of Lactuca sativa rhizosphere microbiota in an aquaponic system
Stouvenakers, Gilles ULiege; Massart, Sébastien ULiege; Jijakli, Haissam ULiege

Poster (2016, May 17)

Aquaponics is an integrated recirculated system that combines aquaculture and hydroponic plant production. The dissolved nutrients generated by the fish rearing after bacterial activities are used by the ... [more ▼]

Aquaponics is an integrated recirculated system that combines aquaculture and hydroponic plant production. The dissolved nutrients generated by the fish rearing after bacterial activities are used by the plants for their growth. This uptake reduces the accumulation of some molecules which allow a longer water recirculation. When comparing growth conditions between aquaponics and hydroponics, we observed much lower concentration of several key nutrients in aquaponics. Nevertheless, several experimentations report similar or better yield in aquaponics compared with hydroponics. One explanation of this phenomenon could be linked to microbial actions. Nowadays, microorganisms related to aquaculture and hydroponics are well characterized but very little is known about the microbiota associated with aquaponic system. The first aim of this study is to characterize the rhizosphere microbiota (metagenomics) of lettuces growing in an aquaponics system. A NGS approach targeting the 16S rDNA will be carried out to evaluate the bacterial composition and diversity. The second aim is to assess the resistance of aquaponics lettuces following a pest introduction. Yields, symptoms and microbial changes due to the pest will be recorded and analysed to determine if aquaponics systems provide a better plant protection than hydroponics. Depending on the results, the final steps will be to identify the optimal conditions for the management of the potential beneficial microbiota or isolate a beneficial microorganism. [less ▲]

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See detailInsights gained from metagenomic sequencing of apple fruit surface (cv. Pinova)
Angeli, Dario; Massart, Sébastien ULiege; Sare, Abdoul Razack ULiege et al

Poster (2016, May 17)

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly ... [more ▼]

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly studied so far. They holistic study was an unattainable objective due to the absence of techniques to survey microbial communities and their evolution. The recent developments in high-throughput sequencing (HTS) have now enabled the holistic analysis of the microbiota genomes. A pioneering assay has been carried out to get insight into the microbiota of apple surface through metagenome sequencing. Apple from Pinova cultivar have been sampled in orchard and the microbiota isolated. After DNA extraction, the HTS assay generated 14.5 Gbases which were assembled in 133,888 contigs. These contigs provided useful information on taxonomic composition of the microbiota. A total of 1398 bacterial species and 397 fungal species have been identified. Among them, 22 species corresponded to known apple pathogens (Penicillium expansum, Penicillium spp, Botrytis cinerea, Monilinia fructicola, Nectria haematococca, Glomerella cingulate, G. graminicola, Alternaria alternate, Alternaria brassicicola, A. citri, A. longipes, A. solani, A. tenuissima, Fusarium solani, F. oxysporum, F. culmorum, F. lateritium, Mucor circinelloides, M. hiemalis, F. racemosus, Venturia inaequalis, Cladosporium cladosporioides). In addition, several contigs were assigned to species or genus of known BCA strains like Pichia canadensis, Bacillus sp., Debaryomyces hansenii, Aureobasidium pullulans and Pantoea vagans. These results will be discussed. In any case, these results underline a very diverse microbial community whose role needs to be characterized. In this line, the functional analysis (gene function determination) of the contigs is ongoing. [less ▲]

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See detailCan tropical basil be integrated in vegetable crop pest management?
Yarou, Boni Barthélémy ULiege; Verheggen, François ULiege; Heuskin, Stéphanie ULiege et al

Poster (2016, May 17)

To address human and environmental health issues related to the use of synthetic pesticides, it is important to explore other ecological pest management approaches. Within this context, a study was ... [more ▼]

To address human and environmental health issues related to the use of synthetic pesticides, it is important to explore other ecological pest management approaches. Within this context, a study was conducted to evaluate the toxic and repellent effect of Ocimum gratissimum L. (Lamiaceae) on Myzus persicae S. (Aphididae). The toxicity of O. gratissimum essential oil (EO) was evaluated on M. persicae comparing 3 concentrations (0.001%; 0.01% and 0.1%) to a control (15% sucrose). Mortality rate and fecundity were assessed for each concentration. The observations were made daily, for 4 days, on 12 replicates per treatment. To test the repellent effect of O. gratissimum plants, 2 treatments were compared: a group of 8 Amaranthus cruentus L. (Amaranthaceae) plants with 1 plant of O. gratissimum in the middle and a group of 9 A. cruentus plants (control). The distribution of aphids was analyzed 12 days after the middle plant infestation (20 aphids per plant) for each treatment (6 replicates per treatment). O. gratissimum EO was found toxic from a concentration of 0.01%, with a mortality rate of 34% versus 12% for the control ; The fecundity felt from 30 larvae after 4 days (control) to 15 larvae for the 3 doses of the EO. The analysis with the generalized linear mixed model with Poisson error distribution followed by Turkey test (5 %) showed that EO is significantly more toxic than the control, both for mortality and fecundity (p <0.001). In association test, the population of M. persicae (15 aphids per plant) was significantly (p<0,001) lower when A. cruentus plants was associated with O. gratissimum plant than with the control (22 aphids per plant). Furthermore, the population increases gradually as one moves away from the infestation point in association test whereas it decreases in the control. With regards to these results, it appears that O. gratissimum has biocide effects on M. persicae. This plant may be used in an integrated pest management strategy in the production of vegetable to reduce the use of synthetic pesticides and avoid chemicals residues. [less ▲]

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See detailVortex Image Processing (VIP) package for high-contrast direct imaging
Gómez González, Carlos ULiege; Absil, Olivier ULiege; Wertz, Olivier ULiege et al

Poster (2016, May 16)

VIP is a Python instrument-agnostic toolbox featuring a flexible framework for reproducible and robust data reduction. VIP currently supports three high-contrast imaging observational techniques: angular ... [more ▼]

VIP is a Python instrument-agnostic toolbox featuring a flexible framework for reproducible and robust data reduction. VIP currently supports three high-contrast imaging observational techniques: angular, reference-star and multi-spectral differential imaging. The code can be downloaded from our git repository on Github: http://github.com/vortex-exoplanet/VIP [less ▲]

Detailed reference viewed: 57 (8 ULiège)
See detailCharacterization of FRD3, a zinc and iron homeostasis actor, in Arabidopsis
Scheepers, Maxime ULiege; Charlier, Jean-Benoit; Spielmann, Julien ULiege et al

Poster (2016, May 13)

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See detailRestricted interests in autism with versus without speech onset delay : the importance of perceptually versus thematically organized interests
Chiodo, Liliane ULiege; Majerus, Steve ULiege; Eusèbe, Sandrine et al

Poster (2016, May 13)

Objectives : To explore whether autistic adults with vs without speech delay also differ in the perceptual vs thematic nature of their restricted interests.

Detailed reference viewed: 39 (8 ULiège)
See detailTailoring γ-Al2O3 supports with specific silicon precursors
Claude, Vincent ULiege; Vilaseca, Miriam; Heinrichs, Benoît ULiege et al

Poster (2016, May 13)

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See detailSingle cell analysis of Escherichia coli outer membrane porin composition in response to nutrient depletion
Delepierre, Anissa ULiege; Brognaux, Alison ULiege; Bauwens, Julien ULiege et al

Poster (2016, May 12)

Characterization of outer membrane integrity into isoclonal population of Escherichia coli using fluorescent probe specific to cell viability: propidium iodide (PI) staining; combined to FACS cytometry ... [more ▼]

Characterization of outer membrane integrity into isoclonal population of Escherichia coli using fluorescent probe specific to cell viability: propidium iodide (PI) staining; combined to FACS cytometry and proteomic studies of sorted subpopulations. The results tend to reveal two distincts cellular strategies: Cells positively probed by PI offer a high outer membrane protein (OMP) content, indicating nutrient competence in response to substrate limitative conditions. Unprobed cells, characterized by a low OMPs rate, could suffer from growth arrest and develop stress responses (Crp, Cra, RpoS,RpoN, RpoH-dependent). [less ▲]

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See detailMolecular detection of single nucleotide polymorphisms on whole blood without DNA extraction
Detemmerman, L.; BOEMER, François ULiege; SEGERS, Karin ULiege et al

Poster (2016, May 12)

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See detailSpatial Analysis of the Landslide Risk in the Cameroon Volcanic Line (CVL)
Mukenga, Williams ULiege; Havenith, Hans-Balder ULiege; Medjo Eko, Robert et al

Poster (2016, May 12)

Landslides are recognized as important geomorphologic process due to the role they play in the development of hill slopes in mountainous regions, and to related socio-economic consequences. There are many ... [more ▼]

Landslides are recognized as important geomorphologic process due to the role they play in the development of hill slopes in mountainous regions, and to related socio-economic consequences. There are many causes of landslides and their distribution varies with the changing conditioning factors. Slope stability depends on a number of causative factors and the knowledge of these variables can help to predict the type of landslide expected in the future. In this study, past landslide activity and triggering factors will be used to assess mass movement hazards along the Cameroon Volcanic Line (CVL). Spatial aspects will be studied in terms of landslide susceptibility maps that are important for development planning and disaster management. The current synthesis of landslide susceptibility map at regional scale is based on a literature review to inventory passed mass movement impacts, on the detection of landslide features from Google Earth imagery, combined with spatial analysis using Geographical Information Systems (GIS) and remote sensing. One of the most critical stages in landslide susceptibility mapping is the selection of landslide pre-conditioning, conditioning and triggering factors and weighting of the selected causative factors in accordance to their influence on slope stability. Google earth helps for delineating past (but recent) and present landslide activity whereas GIS is suitable when deriving static factors (slope aspect and surface curvature) and time-dependent factors (annual precipitation and changing groundwater table level) that are needed to produce landslide susceptibility maps. Landslide susceptibility mapping is based on the assumption that future landslides will occur under similar circumstances as past and present mass movements. Different methods, including the weight of evidence methods, are used for landslide susceptibility mapping along the Cameroon Volcanic line, as the area is known to be highly susceptible to landslide occurrences. [less ▲]

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See detailPRESENCIA, COMPORTAMINETO Y RIESGO AMIBIENTAL DE LOS PRODUCTOS DE CUIDADO PERSONAL (PCPs) EN LA INTERFAZ RÍO-ACUÍFERO
Serra-Roig, M. Pau; Jurado Elices, Anna ULiege; Diaz-Cruz, M. Silvia et al

Poster (2016, May 12)

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See detailOzone concentration and CO2 flux monitoring in a belgian forest
Hurdebise, Quentin ULiege; Bergmans, Benjamin; Aubinet, Marc ULiege

Poster (2016, May 11)

Temperate forests, as other ecosystems and oceans, mitigate the accumulation in the atmosphere of CO2, the main responsible for the current climate change. It is, therefore, a necessity to understand how ... [more ▼]

Temperate forests, as other ecosystems and oceans, mitigate the accumulation in the atmosphere of CO2, the main responsible for the current climate change. It is, therefore, a necessity to understand how these forests react and will react in a changing environment. That requires long-term monitoring of the environment. Tropospheric ozone is one parameter to monitor as it affects the forest by inducing oxidative stress once inside the leaves. Using 16 years of continuous data from an air quality monitoring station managed by the ISSeP (Institut Scientifique de Service Public) located in a mixed forest in east Belgium, we identified ozone concentration extreme events and we investigated their occurrence frequency as well as their relation with climatic variables and air components. As variables are subjected to daily, yearly and inter-annual dynamics, anomalies were used to detect extreme events. To obtain anomalies, we removed the previously mentioned dynamics from half-hourly raw data. Daily and yearly dynamics were removed first and explained 40% of the variability observed in half-hourly ozone concentrations. Then, the inter-annual dynamic was obtained by computing the yearly mean of the residuals, it accounted for 2% of the variability observed. A significant (p<0.01) decreasing trend was observed for these residuals. Determining if that trend is site specific or regionally observed can be done by applying the same analysis to the other sites from the network managed by the ISSeP. No significant trend in the yearly frequency and intensity of extreme events was observed. We used a stepwise regression to identify the variables that explain these extreme events. Significant relations were found between ozone concentration and climatic variables (vapor pressure deficit, temperature, radiation and wind direction) as well as other air components concentration (NO2, NO, NOX, CO). The next step will be to estimate the forest capacity to uptake ozone in order to predict ozone-induced damage and compared it to the CO2 uptake and H2O emissions measured in an ICOS site, the Vielsalm Terrestrial Observatory, located in the same forest at less than 300 meters from the air quality monitoring station. [less ▲]

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See detailAccurate measurement of radius evolution as a function of direction in 3D images
Plougonven, Erwan ULiege; Hubert, Julien ULiege; Collin, Frédéric ULiege et al

Poster (2016, May 11)

Very often the reason for using of X-Ray tomography in a research project is to measure the evolution of some geometrical characteristic. The sought characteristic may be explained in simple words like ... [more ▼]

Very often the reason for using of X-Ray tomography in a research project is to measure the evolution of some geometrical characteristic. The sought characteristic may be explained in simple words like size, shape, or distance, but in practice measures based on these notions can have a complex implementation. We present a simple problem, measuring the evolution of radius in a cylindrical sample (a common shape for sample preparation for tomography) and how this value differs according the direction in which we look, when such evolution is known to be anisotropic. The solution is not unique, but we show that a naïve manual approach are not accurate enough, and how even a simple geometric notion such as radius needs a thorough definition in relation to its applicability to 3D image analysis. We extend the argument to how a good understanding of the notions and algorithms used in the quantification of geometrical characteristics can directly affect the pertinence and representativity of the results. [less ▲]

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See detailAssociation of Targeted metagenomic analysis and classical microbiology for Clostridium difficile detection and microbial ecosystem mapping of surfaces hands and foodstuffs in a meat processing plant
Rodriguez Diaz, Cristina ULiege; Oukbir, Meriem; Taminiau, Bernard ULiege et al

Poster (2016, May 11)

INTRODUCTION Zoonoses are infectious that can be transmitted between animals and humans through direct contact, close proximity or the environment. Since domestic and food animals frequently test positive ... [more ▼]

INTRODUCTION Zoonoses are infectious that can be transmitted between animals and humans through direct contact, close proximity or the environment. Since domestic and food animals frequently test positive for the bacterium, it seems plausible that C. difficile could be zoonotic. PURPOSE This study aimed to determine the prevalence and the epidemiology of C. difficile in cattle farms and the possible spread of the bacterium among animals and farmers. METHODS A total of 176 faecal samples of cattle were collected from 5 different Belgian farms. A stool sample of each farmer was also requested. Detection of C. difficile was performed by classical culture on C. difficile selective medium (cycloserine cefoxitin fructose cholate). Isolates were characterised by PCR-ribotyping and Genotype Cdiff test (Hain Lifescience), which allows the detection of all toxin genes, mutations in gyrA gene and the deletion in the regulator gene tcdC. RESULTS C. difficile was detected in 14/178 (7.9%) animal samples. Isolates were grouped into five different types, including PCR-ribotype 015. All of them were identified as toxigenic. In contrast, none of the 5 farmers studied were positive for the bacterium. SIGNIFICANCE Results obtained indicate that PCR-ribotypes commonly isolated from hospitalised patients are also present in cattle, indicating an animal reservoir. However, a zoonotic transmission could be not demonstrated in the farms studied. [less ▲]

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