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See detailTesting the impact of stratigraphic uncertainty on spectral analysis of sedimentary time series
Martinez, Mathieu; Kotov, Sergey; De Vleeschouwer, David et al

Poster (2015, April)

Spectral analysis has become a key tool for identifying the imprint of astronomical forcing on sedimentary records. In a next step, the identified cycles often contribute to the construction of a precise ... [more ▼]

Spectral analysis has become a key tool for identifying the imprint of astronomical forcing on sedimentary records. In a next step, the identified cycles often contribute to the construction of a precise Geological Time Scale and to an in-depth understanding of past climate changes. Most of spectral analyses (Fast Fourier Transforms, the Multi-Taper Method. . . ) require a constant sample step. Unfortunately, an equally spaced geological data series is, in practice, nearly impossible to obtain from field sedimentary series. Usually, there is a 10% uncertainty on the field measurements of the stratigraphic thickness within sedimentary series. Hence, important uncertainties exist on the actual position of each sample. Another source of uncertainty are errors in a time-space model. In this study, we explore the impact that the stratigraphic uncertainty on the sample position has on the result of spectral analyses. To simulate this uncertainty, we developed a model based on the Monte Carlo randomisation of the distance between each successive point. In this way, the stratigraphic order of the data points is not affected after imp lementating this model. The application of this model to a theoretical sinusoid series and to several real sedimentary series shows that uncertainties in the actual position of samples can highly reduce the spectral powers of the frequencies ranging from the Nyquist Frequency up to 1/10 of the Nyquist Frequency. We the demonstrate that the precise reconstruction of the Milankovitch cycles in the sedimentary record requires a higher sampling density than previously suggested with, at least, 10 samples per thinnest cycle to be detected, i.e. 10 samples per precession cycle [less ▲]

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See detailTillage as a tool to manage crop residue : impact on sugar beet production
Hiel, Marie-Pierre ULg; Degrune, Florine ULg; Parvin, Nargish ULg et al

Poster (2015, April)

Crop residues and plant cover represent a pool of organic matter that can be used either to restore organic matter in soils, and therefore maintain soil fertility, or that can be valorized outside of the ... [more ▼]

Crop residues and plant cover represent a pool of organic matter that can be used either to restore organic matter in soils, and therefore maintain soil fertility, or that can be valorized outside of the field (e.g. energy production). However, it is crucial that the exportation of residues is not done to the detriment of the system sustainability. Three long term experiments have been settled in the loamy region in Belgium. All of them are designed to study the effect of residues management by several tillage systems (conventional plowing versus reduced tillage) on the whole soil-water-plant system. SOLRESIDUS is a field experiment where we study the impact of crop residue management while in SOLCOUVERT and SOLCOUVERT-BIS, we study the impact of cover crop management. SOLRESIDUS was started in 2008. In this field, four contrasted crop residues managements are tested in order to contrast as much as possible the responses from the soil-water plant system. Two practices characterize the four modalities: soil tillage (ploughing at 25 cm depth or reduce tillage at 10 cm max) and residue management (exportation or restitution). SOLCOUVERT and SOLCOUVERT-BIS were started in 2012 and 2013 respectively. In those fields cover crop management is also diverse: destruction of the cover crop by winter ploughing, spring ploughing, strip tillage (with a chemical destruction if needed) or shallow tillage (with a decompaction before cover crop sowing). Although although the overall project aims at studying the impact of management on the whole soil-water-plant system, here we will only present the results concerning crop production (sugar beet) in SOLCOUVERT experiments. The presented data will include germination rate, crop development (biomass quantification and BBCH stages) weeds population, disease occurrence, pest occurrences, nitrogen uptake by plants, quality and quantity of harvested products.   [less ▲]

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See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Hubert, Sandrine ULg; Bruns, Carson et al

Poster (2015, April)

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See detailSoil microbial community composition changes according to the tillage practice and plant development stage
Degrune, Florine ULg; Dufrêne, Marc ULg; Taminiau, Bernard ULg et al

Poster (2015, April)

Agricultural practices have a strong impact on soil bacterial and fungal community composition. Furthermore, microbial community composition can change with the stage of plant development. We are ... [more ▼]

Agricultural practices have a strong impact on soil bacterial and fungal community composition. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these effects in relation to changes induced by agriculture (conventional and reduced tillage) and plant stage (germination and flowering) in soil conditions. Here, instead of examining this impact at a high taxonomic level such as phylum and/or class, thus missing potentially relevant information from lower levels, we propose an original method: exploiting the available sequence information at the lowest taxonomic level attainable for each operational taxonomic unit. Results show that some microbial communities were impacted only by the tillage practice , while others were impacted only by the stage of plant. Changes in microbial community composition could be due to the soil conditions induced by the soil practice and the stage of plant. [less ▲]

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See detailCombining tree-based and dynamical systems for the inference of gene regulatory networks
Huynh-Thu, Vân Anh ULg; Sanguinetti, Guido

Poster (2015, April)

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See detailDecrease of the Black Sea Oxygen Inventory through the second half of the XXth century
capet, arthur; stanev, Emil; Grégoire, Marilaure ULg

Poster (2015, April)

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See detailInfusion of third-party mesenchymal stromal cells after liver transplantation: a phase 1, open-label, clinical study
DETRY, Olivier ULg; VANDERMEULEN, Morgan ULg; DELBOUILLE, Marie-Hélène ULg et al

Poster (2015, March 27)

Transplanted patients have to deal with numerous side effects of life-long dependence on immunosuppressive drugs. Paradoxically these drugs fail to prevent acute and/or chronic rejection in many cases ... [more ▼]

Transplanted patients have to deal with numerous side effects of life-long dependence on immunosuppressive drugs. Paradoxically these drugs fail to prevent acute and/or chronic rejection in many cases. Mesenchymal stromal cells (MSC) are multipotent and self-renewing bone marrow progenitors that have been shown both in vitro and in vivo as capable of (i) immunomodulation, (ii) anti-inflammation in case of ischemia/reperfusion injury, and (ii) stimulation of tissue repair. MSC could therefore be very interesting in organ recipients to limit chronic graft damage and to allow tolerance. This study aimed to be the first clinical evaluation of the safety and tolerability of MSC infusion after liver transplantation in a prospective, controlled, phase I study. Clinical grade MSCs were locally collected from the bone marrow of unrelated healthy donors. They were cultured in a GMP-compliant lab, underwent extensive quality controls and were frozen for storage in a MSC bank. When needed for patient treatment, MSC were thawed and intravenously injected into patients. 10 liver transplant recipients under standard immunosuppression (TAC-MMF-low dose steroids until day 30) received 1.5-3x106/kg MSC on post- operative day 3 ± 2. These patients were prospectively compared to a group of 10 control (MSC-) liver recipients. Primary endpoints were MSC infusion toxicity, and incidence of cancer and opportunistic infections at month 6. Secondary endpoints were patient and graft survivals and rejection at month 6, as well as the effects of MSC on recipients’ immune function and on immunohistology of at month 6 graft biopsies. No MSC infusional toxicity was observed. Both groups were comparable in terms of donor and recipient characteristics. There was no difference in primary end-points between control and MSC groups. No patient developed de novo cancer. There was no statistical difference in patient and graft survivals or in rejection rates. There was no graft rejection in the MSC group. Month-6 graft biopsies were not different according to Banff and fibrosis scores. This phase I study showed excellent tolerability and safety of a single infusion of third-party MSC after liver transplantation. There were no graft safety issues and no excess of immunosuppression after MSC injection. Further analyses of consequences of MSC injection on the immune profile are needed. The possibility of avoiding calcineurin-inhibitors with repeated MSC injections as main immunosuppressive therapy and/of tolerance induction by MSC infusion should be investigated by further studies. [less ▲]

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See detailApplications of stable isotopes in environmental studies at the University of Liege
Sturaro, Nicolas ULg; Borges, Alberto ULg; Das, Krishna ULg et al

Poster (2015, March 26)

Measurement and use of stable isotope ratios have a long history at the University of Liege (Belgium). Since at least 30 years, applications of stable isotopes in marine ecosystems have been developed ... [more ▼]

Measurement and use of stable isotope ratios have a long history at the University of Liege (Belgium). Since at least 30 years, applications of stable isotopes in marine ecosystems have been developed within the Laboratory of Oceanology and, more recently, within the Chemical Oceanography Unit. In the Laboratory of Oceanology, one research axis is the measurement of stable isotope composition (C, N, S) in organic matter to delineate trophic web structure and to study animal diet, their trophic niches and their alteration by human activities. This methodology has been successively applied worldwide in different habitats and ecosystems (marine, freshwater, terrestrial) in temperate and tropical areas. Mediterranean food web and fish trophic ecology have received a particular attention. Coupling between trophic ecology and ecotoxicology is another area of investigation. This has been applied mainly to marine vertebrates and freshwater ecosystems. Stable isotope labelling is also used in our laboratory to study and quantify various ecological processes such as inorganic nitrogen incorporation and trophic transfers. The laboratory facilities, renewed in 2012 and managed by Dr. Gilles Lepoint, are composed of an elemental analyser (EA, vario MICRO cube, Elementar) and a gas chromatography (GC, Agilent) coupled to an isotope ratio mass spectrometer (IRMS, Isoprime 100). The GC is also equipped with a quadrupole mass spectrometer. In 2014, the Chemical Oceanography Unit, headed by Dr. Alberto Borges, has acquired and implemented an off-axis cavity ring-down spectroscopy (CRDS) for the measurements of δ15Nα, δ15Nβ, δ18O of N2O. This enables characterization of the N2O origin in a variety of aquatic environments including groundwater in Wallonia, rivers and lakes in Wallonia and Africa, coastal environments (Scheldt estuary, Lake Grevelingen, North Sea), Mediterranean seagrass beds, and Antarctic and Arctic sea-ice. [less ▲]

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See detailUse of C, N and S stable isotope ratios to highlight resource segregation among hermit crabs from tropical seagrass meadows
Michel, Loïc ULg; Frederich, Bruno ULg; Lavitra, Thierry et al

Poster (2015, March 26)

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local ... [more ▼]

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local populations. Meadows however undergo multiple threats linked to human activities (increased nutrient input, overfishing, invertebrate overharvesting, etc.). It is currently hard to assess how seagrass meadows could respond to anthropogenic impacts due to poor knowledge of their functional ecology. In an effort to unravel trophic interactions ruling the food webs associated to seagrass beds of the Toliara Great Reef (SW Madagascar), we studied resource segregation between two common Diogenidae hermit crabs (Dardanus scutellatus and Ciliopagurus tricolor) using stable isotope ratios. C, N and S stable isotope ratios of bulk muscle tissue were measured via CF-EA-IRMS (Elementar Vario MicroCube EA coupled to an Isoprime 100 MS). Interspecific differences were noted in isotopic composition of carbon (δ13C = -12.22 ± 1.73 ‰ for D. scutellatus, δ13C = -14.55 ± 0.73 ‰ for C. tricolor), nitrogen (δ15N = 4.73 ± 0.53 ‰ for D. scutellatus, δ15N = 5.20 ± 0.61 ‰ for C. tricolor) and sulfur (δ34S = 14.08 ± 2.32 ‰ for D. scutellatus, δ34S = 16.73 ± 1.49 ‰ for C. tricolor), suggesting that the two species do not feed on the same items. In addition, SIBER (Stable Isotope Bayesian Ellipses in R) modeling based on C and N data clearly showed that no overlap was present in the core isotopic niches of the two species. It also indicated that the isotopic niche of D. scutellatus was greater than the one of C. tricolor, implying that the former feeds on a greater number of items than the latter. While hermit crabs are generally considered as omnivorous species, this study highlighted differences in the foraging ecology of D. scutellatus and C. tricolor. These differences could help to limit competition for food between these two species, and facilitate their coexistence in Malagasy seagrass beds. [less ▲]

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See detailEvaluation of pitch accuracy in solfeggio examinations: What about non-musical variables?
Larrouy, Pauline ULg

Poster (2015, March 24)

Background and aims In experimental settings, 81% of the variance of judges’ rating of pitch accuracy is explained by musical variables (precision of pitch intervals and respect of tonality) (Larrouy ... [more ▼]

Background and aims In experimental settings, 81% of the variance of judges’ rating of pitch accuracy is explained by musical variables (precision of pitch intervals and respect of tonality) (Larrouy-Maestri, Lévêque, Schön, Giovanni, & Morsonne, 2013). In ecological settings, non-musical variables influence judgments of music performances (i.e., McPherson & Thompson, 1998; Platz & Kopiez, 2012). This study aims to better understand the evaluation of pitch accuracy in the context of formative and summative solfeggio examinations at tertiary music level, for which live performances are evaluated. Method Twenty-one participants of conservatory were asked to learn simple melodies during solfeggio classes. They were evaluated two times (formative and summative examinations) by 3 judges. Each performance was also objectively analyzed regarding pitch accuracy (number of contour errors, precision of pitch intervals and respect of the tonal centre) with a computer-assisted method (Larrouy-Maestri & Morsomme, 2014). Results The 3 judges provided strongly and significantly correlated ratings. The musical criteria objectively analyzed explained 56% of the variance of the jury’s rating when the examination purpose was formative (p < .001) and 31% of the variance when the purpose was summative (p = .009). Interestingly, the predictive musical criteria differed depending on the examination’s purpose. In addition, the variance explained by our statistical model increased (from 56% to 67% and from 31% to 46%) when taking into account non-musical variables such as the gender of the music students. Conclusions Besides the educational perspectives, the proposed method appears interesting for examining the influence of non-musical variables on the pitch accuracy assessment in ecological contexts. [less ▲]

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See detailModelling of large-scale in situ ventilation test in clayey rock
Pardoen, Benoît ULg; Collin, Frédéric ULg; Talandier, Jean

Poster (2015, March 23)

see attached file

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See detailNiche modeling within and between species in the genus Erythrophleum : intergating phylogenetic data and environmental niche models to explore speciation mechanisms
Gorel, Anaïs ULg

Poster (2015, March 21)

In this study we investigated the climatic niche between and within closely related tropical tree species of the Erythrophleum genus (Fabaceae, Caesalpinioideae): E. ivorense, E. suaveolens and E ... [more ▼]

In this study we investigated the climatic niche between and within closely related tropical tree species of the Erythrophleum genus (Fabaceae, Caesalpinioideae): E. ivorense, E. suaveolens and E. africanum. Two major hypotheses, the refuge theory and the ecological gradient hypothesis, have been developed to explain the current distribution of tree clades across tropical Africa. To identify the speciation mechanisms, we used a combination of geographic data and environmental factors to quantify the degree of niche conservatism (or divergence). We used two sets of distribution data for the purpose of this study. Species distribution data for the whole of tropical Africa was gathered from herbarium records. Distribution data of the two sister species E. ivorense and E. suaveolens assigned to genetic cluster were available for the lowland tropical forests of western and central Africa. Using a Species Distribution Model approach based on MaxEnt algorithm we tested for the environmental differences between species and genetic clusters within species. At species level, the climatic niche significantly differed and only slightly overlapped, suggesting a parapatric speciation along a climatic gradient. Within the two sister species, the niche of the parapatric central African genetic clusters suggests mostly a secondary contact following the recolonization from different forest refugia. [less ▲]

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See detailImproving knowledge on Forest elephant’s ecophysiology (Loxodonta africana cyclotis) for better wildlife conservation
Ngama, Steeve ULg; Vermeulen, Cédric ULg; Bindelle, Jérôme ULg

Poster (2015, March 21)

Conservation of large wildlife species is currently a major issue in Africa. Protected areas dedicated for biodiversity conservation unfortunately do not suffice and conservation practices must be ... [more ▼]

Conservation of large wildlife species is currently a major issue in Africa. Protected areas dedicated for biodiversity conservation unfortunately do not suffice and conservation practices must be extended to man-used areas. But in those areas conservation actions are limited because of conflicts between human and wildlife especially due to damages on crops. The worst crop raiders known are elephants because they can destroy the yearly harvest of a field in a single visit. This threatens not only people livelihoods but also elephants themselves when angry farmers retaliate by shooting or trapping them. After decades of investigations crop raiding drivers related to elephants’ ecophysiology remain largely unknown. A pilot study was conducted between July and November 2014 in Monts de Cristal National Park (Gabon, central Africa) to have a first view on environmental drivers to crop raiding. While the presence of some fruiting trees around crop fields lead to more damages, high slopes discouraged elephants. In further experiments, the link between the nutritive value of raided plants and the animal’s physiological requirements and status will be assessed through hormones and parasites measurements; while tracking of individual elephants’ movements using DNA analyses in feces will be done. [less ▲]

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See detailNineteenth century human history explains the dominance of light-demanding tree species in Central African moist forests
Morin, Julie ULg; Fayolle, Adeline ULg; Favier, Charly et al

Poster (2015, March 21)

The canopy of central African moist forests is dominated by light-demanding trees. Most of these species show a distribution of diameters that indicates a regeneration shortage. Here we show through the ... [more ▼]

The canopy of central African moist forests is dominated by light-demanding trees. Most of these species show a distribution of diameters that indicates a regeneration shortage. Here we show through the combined analysis of botanical, palaeoecological, archaeological and historical data that most of these trees are not older than ca. 180 years. This age corresponds to the early 19th century (around 1830) when the slave-raiding, the interethnic wars and the colonization of the inlands by the Europeans disturbed the human spatial occupancy. After 1885, the spatial clumping of people and villages along the main communication axes induced less itinerancy in the forest. We believe that former activities such as shifting cultivation created scattered openings in the canopy, large enough to allow light-demanding trees to establish. Nowadays, common logging operations do not create openings sufficiently large for the regeneration of these high value timber species. Our findings emphasize the need to include considerations about the history of human spatial occupancy and activities to understand forest dynamics. We need silvicultural guidelines adapted to the autecology of the species. Population enforcements (e.g. enrichment) will be needed to ensure the sustainability of timber yields in forests dominated by long-lived light-demanding trees. [less ▲]

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See detailThe role of tree species diversity in drought resistance of oak and beech sapling
Rahman, Md Masudur ULg; Verheyen, Kris; Carnol, Monique ULg

Poster (2015, March 21)

Drier condition during the growing season have been predicted in the future. It has been suggested that diverse forest could maintain productivity and provide better ecosystem services under stress ... [more ▼]

Drier condition during the growing season have been predicted in the future. It has been suggested that diverse forest could maintain productivity and provide better ecosystem services under stress condition such as drought. However, those studies focused mainly on mature forest and little known about young forest. Oak and beech are the important species in European forestry, and may face a strong challenge in the future. Drought effects on young ( ̴5yr) oak and beech saplings in monoculture and mixed with other species are not known. Moreover, single studies evaluating both above- and below-ground ecosystem response to drought are scarce. A two-year manipulative field experiment has been planned to answer the following questions. (i) Can species mixtures improve oak and beech sapling performances under drought conditions? (ii) What are the mechanisms underlying ecosystem functioning and sapling performance in mixed species stands subjected to drought? A 3m × 3m rainout shelter will be placed only in growing season in Zedelgem sites of FORBIO experimental platform (http://www.treedivbelgium.ugent.be/pl_forbio.html). Tree diversity vary from 1 to 4 species and about 50% of precipitation will be taken off. Both aboveground sapling performance and belowground microbial properties and biogeochemical processes will be investigated. We will present the design of the experimental tree species diversity plantation of Zedelgem site, the setting of the drought experiment and planned analysis [less ▲]

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See detailInternational Classification of Primary Care in a cross-lingual terminology portal
Jamoulle, Marc ULg; Pizzanelli, Miguel; Grosjean, Julien et al

Poster (2015, March 20)

International Classification of Primary Care in a cross-lingual terminology portal M. Jamoulle1, M. Pizzanelli2, J. Grosjean 3 , G. Kerdelhué 3, SJ. Darmoni 3&4 1 Department of General practice, Liege ... [more ▼]

International Classification of Primary Care in a cross-lingual terminology portal M. Jamoulle1, M. Pizzanelli2, J. Grosjean 3 , G. Kerdelhué 3, SJ. Darmoni 3&4 1 Department of General practice, Liege University, Belgium 2 Unidad docente asistencial rural de Florida, Uruguay 3 Deparment of Biomedical Informatics, Rouen University Hospital, Normandy & TIBS, LITIS EA 4108, France 4 LIMICS, INSERM, U1142, Paris, France. ICPC is available in the HeTOP cross-lingual terminology portal (URL: www.hetop.eu) [1] in 19 languages, mostly European (e.g. Spanish, Portuguese) but also in Japanese or Mandarin. The HeTOP interface has been also translated in 10 languages, including by Wonca colleagues (in Turkish, Vietnamese and Romanian). This is very important for non-English speakers to access a health Web site in his/her native language. Thanks to a partial manual mapping between ICPC2 and MeSH, it is now also possible to query PubMed from 20% of ICPC2 codes. This task is time consuming as in lot of cases, the mapping is 1 to N (one ICPC code generates several MeSH terms; e.g. the ICPC term "diverticular disease" is mapped to two MeSH terms " diverticulosis, colonic" and " diverticulitis". HeTOP currently contains 56 health terminologies and ontologies (only 17 are included in UMLS as most of them are French terminologies), 1,951,834 concepts, 6,636,000 terms, 8,023,181 relations and 1,340,855 relations. Overall, HeTOP contains 108 millions of SPARQL triplets. These figures underlines the possible application of the so called "health big data". Overall, the number of distinct UMLS concepts with at least one French translation in UMLS (MeSH, MedDRA, WHOART, ICPC) is 45,405 vs. 317,539 in HeTOP. 1. Grosjean, J; Merabti, T; Griffon, N; Dahamna, B & Darmoni, SJ. Teaching medicine with a terminology/ontology portal. Stud Health Technol Inform 2012:180;949-53. [less ▲]

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See detail18F-FMT: a reliable PET tracer for in vivo evaluation of dopaminergic dysfunction in Parkinson’s Disease rat model.
Becker, Guillaume ULg; Bahri, Mohamed Ali ULg; Michel, Anne et al

Poster (2015, March 18)

Objectives: Rat models of Parkinson’s disease (PD), such as lesioned rats with 6-hydroxydopamine (6-OHDA), are useful for studying dopamine (DA)-related functions. 6-18F-fluoro-m-tyrosine (6-18F-FMT) is ... [more ▼]

Objectives: Rat models of Parkinson’s disease (PD), such as lesioned rats with 6-hydroxydopamine (6-OHDA), are useful for studying dopamine (DA)-related functions. 6-18F-fluoro-m-tyrosine (6-18F-FMT) is an effective PET tracer to evaluate of DA terminals integrity and L-aromatic amino acid decarboxylase (AAAD) metabolic pathway. However, there are currently no available quantitative PET studies using 18F-FMT in 6-OHDA lesioned rats. In this context, we investigated the feasibility of in vivo PET study using 18F-FMT on 6-OHDA PD’s model. Methods: 10 µg of 6-OHDA were injected into the right medial forebrain bundle (MFB) of male Sprague-Dawley rats (n=8). As control, sham-treated rats (n=8) were injected with vehicle only but otherwise treated identically. Striatal DA presynaptic activity was assessed by dynamic 18F-FMT-PET. Structural T2-weighted brain images were acquired on a 9.4T MRI and were used for co-registration. After normalization on a MRI template, kinetic analysis was performed by “Patlak Reference” model, using PMOD software. Results: Striatal accumulation of 18F-FMT was observed in rats pretreated with benserazide, a peripheral AAAD inhibitor. As consequence of the 6-OHDA-lesion, significant decrease of 18F-FMT accumulation was recorded in the striatum ipsilateral to the lesion. Lesioned rats had dramatically reduced uptake constant Ki in the ipsilateral striatum compared to the contralateral striatum (p<0.001) and to the ipsilateral striatum of sham-treated rats (p<0.005). The Ki ratio (Ipsi./Contra.) was equivalent to 94% in the sham group and dropped to 41% in the lesioned group. Conclusions: 18F-FMT PET enables us to quantify loss of DA presynaptic function in unilaterally 6-OHDA lesioned rats. These results encourage us to pursue further investigations in a longitudinal way and to monitor the progression of the dopaminergic dysfunction in more moderate and gradual preclinical PD models. [less ▲]

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See detailCould salt-soluble proteins denaturation index (Promatest) of corn grain help to predict in vitro digestibility of dry matter?
Odjo, Djosse Psijus Sylvanus ULg; Abédi, Fidèle; Bukamba, Célestin et al

Poster (2015, March 18)

The Promatest is a quick and reliable test which allows the assessment of the loss of solubility of salt-soluble proteins. It was used as a reference method for the assessment of the thermal shock ... [more ▼]

The Promatest is a quick and reliable test which allows the assessment of the loss of solubility of salt-soluble proteins. It was used as a reference method for the assessment of the thermal shock suffered by grain and is frequently used by the starch industry to monitor starch quality. There is some evidence that protein solubility is associated with energy utilization. It was then believed that the Promatest could be used to predict the nutritional quality of corn grain This study aims to determine if the Promatest could be used to predict the in vitro digestibility of dry matter of corn grain. Almost 80 samples of corn grain dried at different temperature were analyzed for their salt-soluble protein content. The in vitro digestibility of these corn samples was determined with three different in vitro methods which chiefly differ from each other in the time, the buffer and the amount of enzymes used. Results show that there is a strong correlation between the three methods the in vitro digestibility of dry matter determination (r=0.7627, 0.767 and 0.9324). The promatest index seems to be inversely correlated with in vitro digestibility of samples (r = -0.5343, -0.6861 and -0.5921). However, analysis of plots displayed for the promatest index and all in vitro digestibility measures show that Promatest index values of some samples with close in vitro dry matter digestibility can drastically vary suggesting that others factors such as drying temperature and moisture content may be involved. [less ▲]

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See detailElectronic nose Technology for reactor state and biogas quality assessment in anaerobic digestion
Adam, Gilles ULg; Lemaigre, Sébastien; Goux, Xavier et al

Poster (2015, March 17)

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See detailSyntheses of nanogels of ionici liquids via controlled radical cross-linking copolymerization
Weiss-Maurin, Mathilde ULg; Taton, Daniel; Detrembleur, Christophe ULg

Poster (2015, March 17)

Cobalt-Mediated Radical Polymerization (CMRP) is one of the most efficient techniques to form sequenced copolymers between monomers of very different reactivities(e.g. vinyl acetate and acrylonitrile ... [more ▼]

Cobalt-Mediated Radical Polymerization (CMRP) is one of the most efficient techniques to form sequenced copolymers between monomers of very different reactivities(e.g. vinyl acetate and acrylonitrile). CMRP is used here to synthesize nanogels of vinylic ionic liquid monomers using different concentrations of cross-linking agent (here, divinyl imidazolium). The aim is to determine the difference in structure of the nanogels, in order to use them for the preparation of star-like and/or core-shell structures. [less ▲]

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See detailFood Compounds from Meadow Grasshoppers
Paul, Aman ULg; Frederich, Michel ULg; Uyttenbroeck, Roel ULg et al

Poster (2015, March 15)

Eating insects as food, particularly grasshopper is practiced in many cultures throughout the world. Meadow grasshopper (Chorthippus parallelus) is commonly found in Europe and some part of Asia. It is ... [more ▼]

Eating insects as food, particularly grasshopper is practiced in many cultures throughout the world. Meadow grasshopper (Chorthippus parallelus) is commonly found in Europe and some part of Asia. It is already known that grasshoppers of Chorthippus species are consumed as food in countries such as Thailand. With the aim of evaluating the nutritional potential of this insect species the proximate nutritional composition of grasshoppers caught from the local fields was realized. Besides this, the fatty acid profile of extracted lipids, amino acid profile and mineral composition of the insect was also revealed. Results suggest that meadow grasshopper is an excellent source of protein and essential amino acids. Lipids extracted from the grasshopper have an interesting fatty acid composition. Also the grasshopper contains some minerals that are important for body. With such protein content, amino acid profile, fatty acid profile of the lipids and mineral content this grasshopper species could present an interesting alternate to conventional protein sources. [less ▲]

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See detailPhysicochemical properties and thermal behaviour of African wild mango (Irvingia gabonensis) seed oil.
Yamoneka, Juste; Malumba Kamba, Paul ULg; Blecker, Christophe ULg et al

Poster (2015, March 15)

Plants belonging Dacryodes and lrvingia genus have great economic importance in Central and West Africa. lrvingia gabonensis (IG) produces a seed rich in fats, traditionally used as a soup thickener ... [more ▼]

Plants belonging Dacryodes and lrvingia genus have great economic importance in Central and West Africa. lrvingia gabonensis (IG) produces a seed rich in fats, traditionally used as a soup thickener. Fruit of Dacryodes edulis (DS) contains a pulp how are also rich in fat. Its seed constitute a big waste problem in towns and village remains not exploitable by local people however that may be valued as a resource for various components. Some study shows that this seed contented a proportion of oils that would be an alternative in the substitution of diesel relevant to their physicochemical properties. Despite the potential of two different oils, theirs scientific data still lack. In this work, thermo-physical behaviour of those two oils is compared in the aim to improving knowledge about properties of those oils that will promote their valorisation in industry scale. The result of this investigation showed that IGO have a highest proportion of oil content with 72.38% compared to DSO (14.64%). IGO is rich in myristic (49.8%) and lauric fatty acid (37%) but DSO sample was found to possess a higher proportion of linoleic acid (35.13%), palmitic (32.67%), oleic (17.70%) but lower proportion of stearic (9.97%). SFC of IGO was the highest at all temperatures. IGO is solid at room temperature with a complete melting at 40°C but DSO is liquid at room temperature. The polymorphism profile of IGO and DSO shows the 13' form stable during heating and a transition to liquid state without passage at β form. [less ▲]

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See detailEtude morphologique de l'effet de l'irradiation gamma sur les amidons de blé, de maïs et de pomme de terre
Atrous, Hager; Ben Bettaïeb, Nasreddine; Danthine, Sabine ULg et al

Poster (2015, March 15)

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See detailCaractérisation physico-chimique et structurale de la poudre et des ulvanes de l'algue ulva lactuca
Guidara, Mariem; Yaich, Hela; Garna, Haikel et al

Poster (2015, March 15)

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See detailDéveloppement d'un modèle mathématique d'identification des différents tissus externes de son de blé dur et tendre (Triticum durum et Triticum aestivum) basé sur l'étude histologique macroscopique et microscopique et la composition biochimique des fractions
Ben Amouda, Mohamed Salah; Roiseux, Olivier; Olive, Gilles et al

Poster (2015, March 15)

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Le blé sert depuis longtemps de nourriture de base à l’homme pour donner, après mouture, à la farine dans le cas des blés ... [more ▼]

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Le blé sert depuis longtemps de nourriture de base à l’homme pour donner, après mouture, à la farine dans le cas des blés tendres, ou à la semoule dans le cas des blés durs. Le produit obtenu correspond à la majorité de l’albumen. Le son de blé est quant à lui un coproduit de cette transformation. C’est un empilement de différents tissus : péricarpe externe et interne, testa, épiderme et aleurone. Actuellement, la valorisation du son de blé reste faible malgré son potentiel nutritionnel. Or ce potentiel permettrait un développement d’additifs alimentaires à haute valeur ajoutée si un fractionnement correct suivi d’une analyse histochimique complète des factions obtenues pouvait être réalisés. Parmi les méthodes de fractionnement, note choix s’est porté sur le debranning qui se base sur le retrait séquentiel des différentes couches extérieures du blé (voir la communication « Mise au point du fractionnement par debranning des tissus périphériques du blé tendre (Triticum aestivum) et du blé dur (Triticum durum) ». Afin de quantifier l’efficacité du fractionnement, l’investigation de la répartition des tissus au sein des fractions produites a été réalisée par l’étude macroscopique (visuelle) et microscopique (coloration histologique spécifique) des grains et des fractions. Un modèle mathématique qui permet l’appréciation de la distribution des couches dans les différentes fractions a également été développé sur base des bio-marqueurs et de la composition biochimique des fractions. L’étude visuelle macroscopique montre que les grains dans tous les cas attaqués de façon hétérogène et ce en raison de la géométrie particulière des grains de blé. L’étude histologique microscopique montre que, malgré cela, certaines fractions sont enrichies en tissus particuliers. Donc, quelque soit la variété étudiée, les fractions obtenues par debranning présenteront toujours une hétérogénéité de composition tissulaire. Après étude du profil nutritionnel (analyse composition chimique), la variété Karim semble la plus intéressante, car elle est riche en protéines, lipides et minéraux. Au niveau des fractions, pour toutes les variétés, la fraction F1 présente le meilleur potentiel. [less ▲]

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See detailAnhydrous Milk Fat enrichment with 13C-­triacylglycerol tracers: effects on thermal and structural behavior.
Danthine, Sabine ULg; Vors, Cécile; Blecker, Christophe ULg et al

Poster (2015, March 15)

Dietary lipids are incorporated in food products under different types of structures, e.g., as dispersed lipid droplets (in oil -in -water emulsions, like creams) or as a continuous lipid phase (in water ... [more ▼]

Dietary lipids are incorporated in food products under different types of structures, e.g., as dispersed lipid droplets (in oil -in -water emulsions, like creams) or as a continuous lipid phase (in water -in -oil emulsions, like butter for example). The crystallization, melting behavior and polymorphic stability of fats are determined by the behavior of the TAGs they contain. In clinical studies, there is a need to add some 13C TAGs as tracers to the ingested fats in order to track their metabolic fate. However, this procedure could modify the physicochemical properties of the fat. The present study was conducted in the framework of a clinical trial aiming at highlighting the effect of the physical structure of a fat (droplets in 0/W emulsion or bulk) in a meal on the absorption, chylomicron transport and further metabolic handling of dietary fatty acids (1). We therefore monitored the thermal and polymorphic behavior of anhydrous milk fat (AMF) enriched in tracers (a mixture of tripalmitin, triolein and tricaprylin; at 2 different concentrations: 1.5 and 5.7 wt%) using DSC and XRD and further compared it to the native AMF. The addition of 13C TAGs modified the AMF melting profile, especially at high concentration. The enriched AMF was completely melted at around 37°C, i.e. close to the body temperature. However, under some conditions, the AMF enriched in high 13C TAGs concentration remained crystallized at 37°C. Similar trends were observed in both systems (bulk vs emulsified). Moreover, AMF polymorphic behavior was also modified upon tracer addition. While only β’ form was observed in the native AMF, the 13 -form was detected in the AMF containing high 13C TAGs concentration. Importantly, low concentration of tracers should not have high impact on human digestive physiology. However more attention should be paid to physicochemical structure when high concentrations are added. (1) Vors et al. 2013. Modulating absorption and postprandial handling of dietary fatty acids by structuring fat in the meal: a randomized cross -over clinical trial. Am J Clin Nutr, 97(1): 23-36. [less ▲]

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See detailThermal and structural behavior of two lauric fats compared to AMF in bulk and oil-in-water emulsion states
Petrut, Raul Flaviu ULg; Anihouvi, Prudent Placide; Blecker, Christophe ULg et al

Poster (2015, March 15)

In a previous study the thermal and polymorphic behavior of four different industrial lauric fats which are sold under the same commercial description, was compared (1). According to the findings the four ... [more ▼]

In a previous study the thermal and polymorphic behavior of four different industrial lauric fats which are sold under the same commercial description, was compared (1). According to the findings the four fats were split in two groups based on the similarities found at polymorphic level. In the present paper two of these industrial lauric fats (F1 and F2, one from each group) were incorporated into oil -in -water emulsions. The objective of this study was to point out the differences in the crystallization and polymorphic behavior between bulk and emulsified fat. Moreover anhydrous milk fat (AMF) was used in the same way for comparison. For that purpose, the fats were investigated for their thermal and polymorphic behavior by differential scanning calorimetry (DSC) and X-ray diffraction (XRD), both in bulk and emulsified state. The DSC and X -Ray investigations made possible the observation of the differences in the crystallization behavior and kinetics that occurred for those fats in the bulk and emulsified state. 1. Anihouvi, P.P., C. Blecker, A. Dombree, S. Danthine, Comparative Study of Thermal and Structural Behavior of Four Industrial Lauric Fats, Food Bioprocess Technol. 6:3381-3391 (2013) [less ▲]

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See detailMise au point du fractionnement par debranning des tissus périphériques du blé tendre (Triticum aestivum) et du blé dur (Triticum Durum)
Roiseux, Olivier; Ben Hamouda, Mohamed Salah; Olive, Gilles et al

Poster (2015, March 15)

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Depuis longtemps, le blé est broyé pour donner soit la farine dans le cas des blés tendres, soit de la semoule dans le cas ... [more ▼]

Le blé est constitué de trois régions : l’albumen, les enveloppes et le germe. Depuis longtemps, le blé est broyé pour donner soit la farine dans le cas des blés tendres, soit de la semoule dans le cas des blés durs, ceci correspond en fait à la majorité de l’albumen. Le son de blé est un coproduit de cette transformation et consiste en un empilement de différents tissus : péricarpe externe et interne, testa, épiderme et aleurone. A l’heure actuelle, la valorisation du son de blé est limitée malgré son potentiel nutritionnel. Un fractionnement spécifique de ces tissus, conduisant à la production de fractions ciblées à haute valeur ajoutée, permettrait de mieux exploiter ce potentiel. Parmi les méthodes de fractionnement par voie sèche, le debranning permet le retrait séquentiel des différentes couches extérieures du blé mettant en jeu l’abrasion contre une surface abrasive et la friction des grains entre eux. L’étude a porté sur deux variétés de blé tendre (Julius et Sahara) et deux de blé dur (Karim et Razzek). En raison de différences physiques comme la dureté ou la taille et la forme des grains, des cycles de traitement adéquats pour chacun des variétés dans le but de mettre en évidence leurs différences comportementales. Les quatre fractions (F1 ; F2 ; F3 ; F4) obtenues ont alors été analysées statistiquement (moyenne, coefficient de variation, minimum et maximum, intervalles de confiance) afin de juger de la conformité de ces fractions en termes de pourcentage massique par rapport au but désiré. Les moyennes et intervalles de confiance montrent que les fractions développées sont conformes aux couches histologiques d’un point de vue massique, vis à vis des valeurs « objectifs » fixées par rapport à la littérature scientifique. [less ▲]

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See detailTECHNICAL CHALLENGES IN CHOLESTEROL REMOVAL FROM DAIRY PRODUCTS
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, March 15)

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane ... [more ▼]

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane. It has tripartite structure consisting of a monolayer facing the inner triacylglycerol core and an external bilayer. Apart from giving the nutritive value, milk fat globule membrane also contributes towards the techno-functional properties of the dairy products. The milk fat globule membrane is composed of both polar lipids and proteins. Cholesterol is also present in milk fat globule membrane and accounts for about 2% of milk fat globule membrane. Increased consumer awareness has created a perceived demand for low cholesterol products. The World Health Organization and American Heart Association has also recommended that consumers reduce their consumption of saturated fatty acids and cholesterol to lower the risk of coronary heart disease. Therefore, many researchers have developed different methods for reducing cholesterol in dairy and food products. These methods include physical process (vacuum steam distillation, short path molecular distillation and supercritical fluid extraction), biological process (cholesterol reductase and cholesterol oxidase), complexation method (adsorption with saponin, digitonin and cyclodextrin) and chemical process. Most of these methods are very efficient in process of cholesterol removal. However, most of these methods have various drawbacks for application in dairy industry like, formation of toxic oxidation products, denaturation of proteins, loss of low molecular weight triglycerides, loss of volatile components, loss of flavour compounds etc. This leads to loss of nutritive value of dairy products. Thus, the objectives of cholesterol removal from milk and milk products should be, firstly focusing on the maximum cholesterol removal, secondly maintaining the nutritional quality comparable to the control and thirdly the process of cholesterol removal should not compromise the techno functional properties of the dairy products. [less ▲]

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See detailPhysicochemical properties and thermal behaviour of African wild mango (Irvingia gabonensis) seed oil.
Yamoneka, Juste; Malumba Kamba, Paul ULg; Blecker, Christophe ULg et al

Poster (2015, March 15)

African wild mango (lrvingia gabonensis) is now recognized for its numerous food and medicinal uses. This plant produces seeds rich in fat, which is traditionally used as a soup thickener. in the present ... [more ▼]

African wild mango (lrvingia gabonensis) is now recognized for its numerous food and medicinal uses. This plant produces seeds rich in fat, which is traditionally used as a soup thickener. in the present study, the proximate composition of seeds from lrvingia gabonensis is reported. The oil from the seeds was then solvent extracted; its fatty acid (FA) and triacylglycerol (TG) profiles were determined. Besides this chemical characterization, the melting and crystallization behaviour of the extracted fat was studied by pNMR, DSC and X-ray diffraction. The result of this investigation showed that the seeds from lrvingia gabonensis (IG) represent an important source of lipids (69-75%), rich in myristic (49.8%) and lauric acid (37%). This fat can be classified among "lauric fats". IG oil presents a good oxidative stability due to its low content in unsaturated fatty acid. The melting profiles of IG oil indicates that a high amount of fat remains solid at temperatures up to 30°C; the complete melting was detected at around 40C. The most stable polymorph of this fat is the β'1 -form. [less ▲]

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See detailThe living of the green microalga Chlamydomonas reinhardtii in day/night cycles
Willamme, Rémi ULg; Alsafra, Zouheir; Alsafra, Rameshkumar et al

Poster (2015, March 13)

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See detailConcomitant nodal involvement by Langerhans Cell Histiocytosis and Hodgkin Lymphoma
Geurten, Claire ULg; THIRY, Albert ULg; Jamblin, Paul et al

Poster (2015, March 12)

Introduction : Langerhans cell histiocytosis is defined as a clonal neoplastic proliferation of myeloid dendritic cells that upon activation migrate from the mucosal to lymph nodes. Definitive diagnosis ... [more ▼]

Introduction : Langerhans cell histiocytosis is defined as a clonal neoplastic proliferation of myeloid dendritic cells that upon activation migrate from the mucosal to lymph nodes. Definitive diagnosis is made by anatomo-pathological and immunohistochemical analysis. Langerhans cell histiocytosis is rarely, yet not exceptionally, found coexisting with other malignant neoplasms, suggesting it might arise in reaction to the cytokinic secretion of malignant cells. Case : We report the case of a 10-year-old female presenting with an isolated laterocervical lymphadenopathy and a mild general condition alteration tracing back to two months earlier. Nodal biopsy was performed and revealed concomitant involvement by Langerhans cell histiocytosis and Hodgkin lymphoma. Treatment of lymphoma led to the disappearance of the whole symptomatology. Discussion : Literature beholds reports of 30 cases of the simultaneous occurence of Hodgkin lymphoma with Langerhans cell histiocytosis, which is more than fortuitous regarding the low incidence of both diseases. A common etiology could explain such an association, but it might also be possible that background inflammatory cells of Hodgkin lymphoma stimulate the proliferation of Langerhans cells, making it a reactive process when occurring simultaneously with other neoplasms. Clinicians should thus be aware of the possibility of this association and carefully exclude any other life-threatening malignant proliferation when confronted to apparently isolated Langerhans cell histiocytosis. [less ▲]

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See detailGenetic Control of Flowering Time in Arabidopsis: an Interactive Database
Bouché, Frédéric ULg; Lobet, Guillaume ULg; Tocquin, Pierre ULg et al

Poster (2015, March 12)

The transition to flowering is an essential step of the plant life cycle that is tightly controlled by both endogenous and environmental cues. Its regulation is extremely complex and involves hundreds of ... [more ▼]

The transition to flowering is an essential step of the plant life cycle that is tightly controlled by both endogenous and environmental cues. Its regulation is extremely complex and involves hundreds of genes that are part of highly interconnected pathways. Our knowledge of the molecular mechanisms governing the floral induction of Arabidopsis thaliana increases quickly and a significant number of reviews are published every year on this topic. However, most of them focus on a single pathway without highlighting the interconnections existing between them. Furthermore, those reviews become rapidly outdated, since our comprehension of the genetic control of flowering time evolves continuously. Hence, we believe that the current landscape of flowering time research in Arabidopsis misses an exhaustive repository of the genes involved in the control of flowering and their regulatory pathways. Here, we present a new interactive resource built around a curated database of the flowering time genes that brings together multiple pieces of information such as their function, the flowering time phenotype of mutants and overexpressing lines, the related key publications, etc. Our website thus gives access to a curated and exhaustive list of the genes involved in the regulation of flowering time in Arabidopsis as well as the regulatory pathways controlling their expression. Because of its flexibility, the database is highly dynamic and will be periodically updated with the future breakthroughs in this domain. [less ▲]

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See detail“Rhizoponics”: a novel hydroponic rhizotron for root system analyses on mature Arabidopsis thaliana plants
Mathieu, Laura ULg; Lobet, Guillaume ULg; Tocquin, Pierre ULg et al

Poster (2015, March 10)

Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system ... [more ▼]

Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system architecture therefore remains a challenge. In the model plant Arabidopsis thaliana, in vitro culture remains the easiest and preferred method to study root development, which limits the analyses to young seedlings. We present here an innovative design of hydroponic rhizotron (rhizoponics) designed for the root system analysis of adult plants of Arabidopsis thaliana. [less ▲]

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See detailA tale of two anomalies. A paternal duplication and a maternal deletion of 15q13
BULK, Saskia ULg; Decortis, Thierry ULg; Rondia, G et al

Poster (2015, March 06)

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See detailA new case of microdeletion 14q32.3
Uwineza, Annette ULg; BULK, Saskia ULg; CABERG, Jean-Hubert ULg et al

Poster (2015, March 06)

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See detailQuaternary prevention, a GP/FM construct based on doctor-patient relationships. From Wonca world Hong Kong 1995 to Wonca APR Taipei 2015
Jamoulle, Marc ULg; Roland, Michel; Tsoi, Gene

Poster (2015, March 04)

Aims Encounter in General Practice/Family Medicine is a meeting point between illness and disease. Looking at patients and doctors beliefs and attitudes, one can define four fields of activity describing ... [more ▼]

Aims Encounter in General Practice/Family Medicine is a meeting point between illness and disease. Looking at patients and doctors beliefs and attitudes, one can define four fields of activity describing the major working areas in GP/FM. Considering clinical prevention as the management of processes over a length of time through the lens of the patient doctor relationships, one can define four main prevention domains. Methods Using a double entry table, we show how the definitions already published in the Wonca Glossary of general practice fit perfectly to the fields I, II and III of prevention and how the fourth definition was developed and edited. Results The story of the construction of the Quaternary prevention is presented as well as its spread worldwide, through new media, as a core value for many young general practitioners. “Action taken to identify a patient or a population at risk of overmedicalisation, to protect them from invasive medical interventions and provide for them care procedures which are scientifically and medically acceptable ” Born in 1986, presented for the first time in Wonca Hong Kong 1995, developed in Durham USA in 1999 the concept has been edited in 2003 in the Wonca dictionary of general practice . Quaternary prevention has now reached the international community of GPs and is considered as a core value by many Wonca representatives Discussion This approach enables us to clarify the concepts of Primary, Secondary and Tertiary prevention while defining a new one: Quaternary prevention.The latter encompasses the consequences of the encounter between the anxiety of the patient and the uncertainty of the doctor and gives insight into the propensity of this kind of meeting to distil sickness, thus creating false positive with its cohort of avoidable human, social and economic costs and suffering. Quaternary prevention (P4), an answer of family doctors facing overmedicalization aims to protect the patient or population against the danger of medicine. See www.ph3c.org/P4. (Poster in Chinese and English) [less ▲]

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See detailOil content, triglycerides profiles and fatty acid composition of almond kernels of some varieties cultivated in eastern Morocco
Houmy, Nadia ULg; Elamrani, Ahmed; Sindic, Marianne ULg et al

Poster (2015, March 03)

Almond tree "Prunus dulcis" is a fruit tree that belongs to the Rosaceae family. It’s a drought tolerant crop, thanks to ability of these trees to endure high water deficits. This physiological propriety ... [more ▼]

Almond tree "Prunus dulcis" is a fruit tree that belongs to the Rosaceae family. It’s a drought tolerant crop, thanks to ability of these trees to endure high water deficits. This physiological propriety of almond trees is a characteristic that prove its efficiency for valorization of semi-arid areas which are widespread in Morocco. According to Agriculture ministry reports, most of almond plantations are located in mountain areas in the Rif and foothills the high Atlas and in the southern areas in arid or semi-arid climate, but Semi-intensive plantations, conducted using modern techniques exist in regions of Fez, Meknes and Marrakech. The commercial production of Morocco is about 97000 tons in kernels and it’s marketed only locally. In the oriental region, the almond growing area is estimated to 9% of total area of almond cultivation in Morocco. Furthermore, an important new plantation program is implemented by DRA1-Oriental with collaboration of CTB2. The objective of this study is a contribution to strengthen competitiveness of the almond sector in the easthern region of Morocco, and focuses on the physico-chemical characterization of almond oils of some local varieties. Simples of 5 almond varieties (Marcona, Ferragnes-Ferraduel, Fournat and Beldi) were analysed. Results showed that yield of oil from almonds crushed mechanically vary between 46 (Fournat) and 60 % (Marcona) of kernels fresh mass. The fatty acid profile was carried out using GC-FID chromatography analysis, that shows a dominance of oleic acid (C18:1) for all analyzed almond oils; C18:1 contents range between a minimum of 63.54 % for Fournat and a maximum of 72.87 % for Ferragnes-Ferraduel. The O/L ratios range between 2.50 (Fournat) and 4.085 (Ferragnes-Ferraduel) and iodine values varied between 98,42 g I2/100g (Ferragnes-Ferraduel) and 103,89 g I2/100g (Fournat). Chromatographic analysis of triglycerides was carried out by HPLC,and results show tha the analyzed almond oils are characterized by dominance of trioleylglycerol (OOO) that contents range between a minimum of 31.48 % for Fournat’s oil and 43.82% for Ferragnes-Ferraduel’s oil. [less ▲]

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See detailELICITOR SCREENING TO PROTECT WINTER WHEAT AGAINST ZYMOSEPTORIA TRITICI BLOTCH
Le Mire, Géraldine ULg; Siah, Ali; Jijakli, Haissam ULg

Poster (2015, March)

Plant protection strategies are strongly focused today on the development of alternative methods in order to complete or replace conventional chemical inputs. Elicitors of biological origin are ... [more ▼]

Plant protection strategies are strongly focused today on the development of alternative methods in order to complete or replace conventional chemical inputs. Elicitors of biological origin are increasingly considered as a promising tool as biocontrol agents. They offer the possibility, through the induction of plant natural defences, to preventively protect plants against a large spectra of diseases. Multiple elicitors have already been identified since their first discovery in the late 70s, but few research has been focused on crop pathogens although such diseases can strongly impact both yield and grain quality. We describe hereby a method of elicitor screening to protect wheat against Zymoseptoria tritici. Focus is made on the importance of a good disease infection protocol in order to achieve screening tests. [less ▲]

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See detailCharacterization of Venom Peptides using Microfluidic Separation Techniques coupled to Mass Spectrometry (LC-MS and CE-MS)
Degueldre, Michel ULg; Delvaux, Cédric ULg; Far, Johann ULg et al

Poster (2015, March)

More than the half of the principal sub-kingdoms of the animal world contains species that produce venom whose purposes are to immobilize, kill and pre-digest the preys. These venoms represent an ... [more ▼]

More than the half of the principal sub-kingdoms of the animal world contains species that produce venom whose purposes are to immobilize, kill and pre-digest the preys. These venoms represent an exceptionally rich source of various biologically active peptides, both in their structures and their effects, which are more and more useful for human being1. Yet, the total characterization of such complex samples require advanced analytical techniques mainly due to the complexity of the sample (hundreds of compounds), the limited quantities usually available and the presence of numerous PTMs, especially disulfide bridges and specific folding. Here we present a method that combines LC and CE separation techniques coupled to mass spectrometry (MS) to characterize the peptide composition of the snake venom Naja atra. The characterization will not only focus on the toxin sequencing (LC-MS and LC-MS/MS), but will also aim at analyzing the folding of the toxins (CE-MS). To this end, native and reduced/alkylated toxins will be analyzed by both techniques. Final result targets the determination of the global hydrophobic pattern and native tridimensional folding of these strongly reticulated peptides. (1) Richard J. Lewis & Maria L. Garcia, Therapeutic potential of venom peptides. Nature Reviews Drug Discovery 2003, 2, 790-802. [less ▲]

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See detailElongator promotes breast cancer metastasis
Delaunay, Sylvain ULg; Rapino, Francesca ULg; Heukamp, Lukas et al

Poster (2015, March)

Elongator is a protein complex (Elp1-6) involved in diverse cellular processes, such as protein acetylation and tRNA modification and whose function is essential for cell migration and neuronal ... [more ▼]

Elongator is a protein complex (Elp1-6) involved in diverse cellular processes, such as protein acetylation and tRNA modification and whose function is essential for cell migration and neuronal differentiation. Although it is well established that tumor development involves modifications of acetylation-deacetylation dynamics, as well as changes in protein translation, the role of Elongator in tumor initiation and invasion remains to be investigated in vivo. We generated a mouse model in which the Elp3 gene, encoding the catalytic subunit of the complex, is conditionally inactivated in the mammary gland epithelium by using the MMTV-CRE transgenic mouse. The role of Elp3 in tumor development and metastasis formation is then assessed in the PyMT model of invasive breast cancer. [less ▲]

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See detailHow snow affects air-sea ice CO2 fluxes ?
Delille, Bruno ULg; Kotovitch, Marie ULg; Van Der Linden, Fanny ULg et al

Poster (2015, March)

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See detailEtude GEROX : Evaluation du statut de stress oxydant
CHRISTELBACH, SOPHIE; RICOUR, Céline ULg; Dardenne, Nadia ULg et al

Poster (2015, March)

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See detailAlignments of quasar axes with large-scale structures
Hutsemekers, Damien ULg; Braibant, Lorraine ULg; Pelgrims, Vincent ULg et al

Poster (2015, March)

Based on measurements of optical linear polarization of quasars belonging to large groups at redshift z ~ 1.3, we found that quasar spin axes are likely parallel to their host large-scale structures ... [more ▼]

Based on measurements of optical linear polarization of quasars belonging to large groups at redshift z ~ 1.3, we found that quasar spin axes are likely parallel to their host large-scale structures (Hutsemékers et al. 2014). These observations can constrain models of the coevolution of AGN, galaxies and large-scale structures. [less ▲]

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See detailTowards a yeast reference interactome
Desbuleux, Alice ULg

Poster (2015, March)

Despite an increasing number of interactomic datasets already available for model organisms and humans, many aspects remain contradictory, debatable or unclear due to the lack of complete high-quality ... [more ▼]

Despite an increasing number of interactomic datasets already available for model organisms and humans, many aspects remain contradictory, debatable or unclear due to the lack of complete high-quality networks. It has, for instance, been proposed that macromolecule connectivity in interactome maps reflects functional importance, functional relatedness, pleiotropy, implication in diseases, and other important biological characteristics. The most notorious example of such relationships concerns so-called essential genes believed to correspond to highly connected hubs that are critical to network integrity. Such claims have led to debate in the literature because connectivity could also be explained by bias and uneven coverage of the interactome space. To provide fresh insight into these questions, we produced a new, systematic interactome map for S. cerevisiae, organism for which a plethora of systematic interactomic and functional data is available. This alternative view of the interactome network was generated by modifying our screening pipeline based on our empirically-controlled framework. Using a new high-quality ORFeome collection and a new assay version, we systematically performed three replicate yeast-two hybrid screens. This produced a map of 1,200 protein-protein interactions, which, while of similar size as previously published interactome maps, covers the entire proteome without bias. These interactions were subsequently successfully validated using an orthogonal protein complementation assay based on a split Gaussia princeps luciferase. The latest analyses of this new map and progress towards generating a first Yeast Reference Interactome map will be presented. [less ▲]

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See detailCan the exploration of left space be induced implicitly in unilateral neglect?
Wansard, Murielle ULg; Bartolomeo, Paolo; Vanderaspoilden, Valérie et al

Poster (2015, March)

The purpose of the present study was to explore the ability of neglect patients to detect and exploit the predictive value of a cue to respond more quickly and accurately to targets on their ... [more ▼]

The purpose of the present study was to explore the ability of neglect patients to detect and exploit the predictive value of a cue to respond more quickly and accurately to targets on their contralesional side in a Posner spatial cueing task. The majority of the cues (i.e. 80%) were invalid, indicating that the target would appear on the opposite side, although patients were not informed of this bias. Our results demonstrate that some neglect patients were able to extract the cue’s predictability and use it to orient faster toward the left. This cueing effect was present even in patients who were subsequently unable to describe the predictive character of the cues, and thus was not modulated by reportable awareness of the cue-target relation. [less ▲]

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See detailPhotosynthesis-irradiance response curves revealed active sympagic communities in the Weddell Sea Winter, 2013
Rintala, J.-M.; Luhtanen, A.-M.; Enberg, S. et al

Poster (2015, March)

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See detailA two-surface viscoplastic model for saturated clays
Hong, P.Y.; Cui, Y.J.; Pereira, J.M. et al

Poster (2015, March)

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See detailMeasurements of air-ice CO2 fluxes over artificial sea ice emphasize the role of bubbles in gas transport
Kotovitch, Marie ULg; Moreau, Sébastion; Zhou, Jiayun et al

Poster (2015, March)

Detailed reference viewed: 29 (3 ULg)
See detailThe role of sea ice in the carbon cycle of Polar Seas: 1D to 3D modelling
Moreau, S.; Vancoppenolle, M.; Delille, Bruno ULg et al

Poster (2015, March)

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See detailStrain localization modelling around an underground gallery in Mol with an analysis of the contact pressure on the lining
Salehnia, Fatemeh ULg; Dizier, Arnaud; Li, Xiang Ling et al

Poster (2015, March)

Detailed reference viewed: 31 (5 ULg)
Peer Reviewed
See detailEffects of transcranial magnetic stimulation coil orientation and pulse width on short-latency afferent inhibition
Hannah, Ricci; D'Ostilio, Kevin ULg; Goetz, Stefan et al

Poster (2015, March)

Detailed reference viewed: 40 (3 ULg)
See detailBlue sky and green bugs – How physical parameters and algal speciation influence DMSP and DMS profiles in Antarctic winter sea ice
Uhlig, C.; Rintala, J.-M.; Tison, J.-L. et al

Poster (2015, March)

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailDifferential membrane marker expression in adult rodent bone marrow mesenchymal and neural crest stem cells.
Wislet, Sabine ULg; Coste, Cécile ULg; Neirinckx, Virginie ULg et al

Poster (2015, March)

Bone marrow stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy. With regards to recent findings ... [more ▼]

Bone marrow stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy. With regards to recent findings, adult mesenchymal stem cells (MSC) are commonly assimilated to neural crest stem cells (NCSC), both isolated from adult bone marrow. The objective of this study was therefore to highlight significant differences for membrane markers between those two cell types. Using the minimal criteria for defining multipotent mesenchymal stromal cells as previously described by The International Society for Cellular Therapy, we were quite surprised that no significant difference could discriminate MSC from NCSC. To define new markers, we first performed a microarray comparison. Based on those results, we validated selected targets by RT-PCR, then by immunocytochemistry. In parallel, we observed that NCSC had the unique property (compared to MSC) to grow as spheres, which could also be used as a purification protocol for NCSC from adult bone marrow. Altogether, we demonstrated that P75NTR was the most significant discriminating marker between MSC and NCSC, isolated from mouse adult bone marrow, which could be used as selecting marker in an enrichment protocol. Sphere formation could then be used as a purification protocol for NCSC. [less ▲]

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See detailHydro-mechanical simulation in a deep excavation in Boom Clay
Hong, P.Y.; Cui, Y.J.; Pereira, J.M. et al

Poster (2015, March)

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See detailIsolation of cultivable viruses from Antarctic wintertime sea ice
Luhtanen, A.-M.; Bamford, D.; De Jong, J. et al

Poster (2015, March)

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Peer Reviewed
See detailCortical responses to transcranial magnetic stimulation during Non-Rapid Eye Movement Sleep
Gosseries, Olivia ULg; Jaakko, Nieminen; Siclari, Francesca et al

Poster (2015, March)

Transcranial magnetic stimulation coupled to high-density electroencephalography (TMS-EEG) allows for directly and non-invasively stimulating the brain and recording the subsequent cortical response ... [more ▼]

Transcranial magnetic stimulation coupled to high-density electroencephalography (TMS-EEG) allows for directly and non-invasively stimulating the brain and recording the subsequent cortical response. Previous TMS-EEG studies have shown clear-cut differences between conscious and unconscious conditions. When subjects are unconscious, as in non-rapid eye movement (NREM) sleep early in the night (stage N3), anesthesia or coma, TMS typically triggers a stereotypical and local slow-wave response. When they are conscious, as in normal wakefulness and rapid eye movement sleep (REM) sleep, brain responses to TMS are long-lasting, widespread, complex, and differentiated. In this study, we performed TMS-EEG during NREM sleep in stage 2 (N2) and N3 on 7 healthy participants. Brain activity was recorded using a 60-channel TMS-compatible EEG and single-pulse TMS was applied (up to 285 pulses per session) on the superior parietal cortex. After each TMS session (5 to 15 sessions per night), subjects were awakened to ask for a dream report. TMS-EEG responses were analyzed using the global mean field amplitude (GMFA), the perturbational complexity index (PCI), as well as time-frequency measures. Our results show that the TMS-EEG response during N3 is larger and slower than during N2 sleep. The GMFA is higher and PCI is lower in N3 as compared to N2 sleep. Moreover, N3 recordings showed activation at lower frequency bands after TMS as compared to N2 sleep. Additionally, TMS-EEG responses vary depending on whether subjects do or do not report a dream. TMS-EEG might provide valuable information for characterizing neurophysiological fluctuations and levels of consciousness within NREM sleep. [less ▲]

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See detailImaging air filled porosity in sea ice cover: Implication for sea ice permeability and gas exchange at the ice-atmosphere interface
Crabeck, O,; Galley, R,; Else, B, et al

Poster (2015, March)

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See detailInfluences of blubber composition and profile in the assessment of POPs levels in free-ranging cetaceans
Pinzone, Marianna ULg; Budzinski, Hélène; Tasciotti, Aurelie et al

Poster (2015, February 28)

Investigating the food and feeding ecology of free-ranging cetaceans has always been very challenging. Still now, mass stranding events represent almost the only opportunity to collect valid information ... [more ▼]

Investigating the food and feeding ecology of free-ranging cetaceans has always been very challenging. Still now, mass stranding events represent almost the only opportunity to collect valid information on these large and elusive animals. Biopsy darting is a non-lethal tissue sampling technique which permits the collection of tissues from living and healthy individuals. However, important discussions exist about how efficient this method is in chemical analyses where the percentage lipid content of the tissue is of great importance. Biopsies of skin and blubber were conducted on 49 long-finned pilot whales (Globicephala melas), 61 sperm whales (Physeter macrocephalus) and 70 fin whales (Balaenoptera physalus) in the North Western Mediterranean Sea (NWMS) from 2006 to 2013. Lipid content, δ13C, δ15N values and ΣPCBs were analysed and compared with previous studies conducted on stranded and biopsied individuals. Lipids extraction was operated via ASE; δ13C, δ15N values and POPs levels were assessed through IR-MS and GC-MS respectively. δ15N values were 12.2±1.3‰ for sperm whales, 10.5±0.7‰ for pilot whales and 7.7±0.8‰ in fin whales, positioning sperm whales at higher trophic levels. δ13C instead was similar and amounted to −17.3±0.4‰, −17.8±0.3‰ and −18.7±0.4‰ respectively. Pilot whales presented the highest concentrations of ΣPCBs (38666 ± 25731ng.g-1 lw) followed by sperm whales (22849 ± 15566ng.g-1 lw) and fin whales (5721±5180ng.g-1 lw). Lipids percentage differed significantly between species. Sperm whales showed the lowest lipid content with an average of 12±9%, whereas for long-finned pilot whales it was 22±21% and for fin whales 31±14%. Lipid content of the two odontocetes varied between years of sampling, whilst for fin whales remained similar. The PCBs concentrations, especially in sperm whales, were in discordance with previous studies conducted in the same area and our δ13C, δ15N values. We hypothesized that (1) the extreme low lipid content found in the blubber, (2) the extraction procedure and (3) the biopsies technique, could explain such different pattern. Several papers demonstrated how POPs concentrations in cetaceans blubber are strongly influenced by its thickness, stratification and lipid profile. Therefore, the particular characteristics of blubber composition of deep—diving income breeders such as sperm whales, may not allow an efficient representation of POPs concentrations through the use of biopsies. [less ▲]

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See detailTeaching and Training TA
Delvenne, Pierre ULg; Rosskamp, Benedikt ULg; Bütschi, Danielle et al

Poster (2015, February 26)

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See detailLe logiciel PegOpera : un outil pour la gestion intégrée des eaux de surface
Grard, Aline ULg; Everbecq, Etienne ULg; Graindorge, Caroline ULg et al

Poster (2015, February 24)

Le logiciel PegOpera inclut le modèle Pegase (Planification Et Gestion de l’ASsainissement des Eaux) et une interface graphique conviviale. PegOpera est un outil personnalisé utilisé par les gestionnaires ... [more ▼]

Le logiciel PegOpera inclut le modèle Pegase (Planification Et Gestion de l’ASsainissement des Eaux) et une interface graphique conviviale. PegOpera est un outil personnalisé utilisé par les gestionnaires de l’eau et opérationnel dans les champs d’applications de la Gestion Intégrée des Ressources en Eau (assainissement, DCE, NQE, …). L'ANPE s'est doté de cet outil d'aide à la décision afin de mieux remplir ses missions de gestion de la qualité des eaux de surface du bassin versant de la Medjerda. [less ▲]

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See detailPlankton diversity and metabolism in the Congo River during high waters (December 2013) and low waters (June 2014)
Darchambeau, François ULg; Descy, JP; Leporcq, B et al

Poster (2015, February 22)

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Peer Reviewed
See detailAdoption by gay and lesbian parents: a cross-nationale study
Messina, Roberta ULg; D'Amore, Salvatore ULg; Green, Robert-J

Poster (2015, February 13)

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See detailRisk of emergence of a hyperpathogenic bovine leukemia virus by mutation of a single envelope N-linked glycosylation site
De Brogniez, Alix ULg; Bouzar, Amel-Baya; Jacques, Jean-Rock ULg et al

Poster (2015, February 11)

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is ... [more ▼]

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is exemplified by the bovine leukemia virus (BLV) system in which lymphoproliferative disorders develop in ruminants after latency periods of several years. Infection of sheep and cattle with BLV is a model system for the related human T-lymphotropic virus type 1 (HTLV-1) responsible for Adult T-cell Leukemia (ATL). - Aims : The goal of this work is to investigate the role of N-glycans of the viral envelope protein during viral replication and pathogenesis. - Methods and results : Using glycosylation inhibitors and lectins, we showed that N-glycosylation is involved in viral infection (i.e. cell-to-cell fusion). Using reverse genetics of an infectious molecular provirus, we next demonstrated that a particular N-linked envelope glycosylation site (N230) limits viral replication and pathogenicity in vitro and in vivo. We have thus generated a viral mutant that is more pathogenic than the wild type strain. - Conclusions : To our knowledge, this is the first time that a hyperpathogenic BLV has been identified. This unexpected observation has important consequences in terms of disease control and managing. Indeed, during evolution, pathogens and their hosts should achieve an equilibrium allowing the coexistence of the two species. Occurrence of this particular mutation may thus represent a potential threat associated with emergence of hyperpathogenic BLV strains and possibly of new variants of the related HTLV-1. [less ▲]

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, February 11)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailCheckpoints modulation by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein : towards new therapeutic approaches
Carpentier, Alexandre ULg; Barez, Pierre-Yves ULg; Boxus, Mathieu et al

Poster (2015, February 11)

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. We are interested in the mechanisms of cell transformation by HTLV-1 and more particularly in the interplay between the viral Tax oncoprotein and the DNA damage response (DDR). We demonstrate that transient expression of Tax results in DNA damage, cell cycle arrest and activation of the ATM-Chk2-p53 axis of the DDR. In fibroblasts, cell cycle arrest occurs at the G1 and G2 phases depending on the p53 background. Despite Tax expression hampers cell cycle progression, neither pro-apoptotic nor pro-senescent effects are observed. In contrast, HTLV-1 infected lymphocytes proliferate continuously and appear to be adapted to the checkpoints. This mechanism allows infected lymphocytes to proliferate despite the presence of genomic lesions. Those cells might thus rely on effective DNA repair mechanisms. Indeed, we show that Tax expressing cells activate the error free repair mechanism homologous recombination (HR). Inhibition of ATM, involved in DDR and DNA repair by HR, impedes Tax-mediated cellular transformation. Depending on these observations, we propose a novel therapeutic approach based on the principle of synthetic lethality. [less ▲]

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See detailThe phosphorylation of RelA on Ser547 does not modulate NF-kB activation after TNFa treatment like after a genotoxic stress
Trussart, Charlotte ULg; Orban, Tanguy ULg; Di Valentin, Emmanuel ULg et al

Poster (2015, February 06)

NF-kB controls the expression of hundred of genes involved in inflammatory and innate responses, proliferation, survival, cancer initiation and progression. Numerous post-translational modifications of ... [more ▼]

NF-kB controls the expression of hundred of genes involved in inflammatory and innate responses, proliferation, survival, cancer initiation and progression. Numerous post-translational modifications of p65 modulating NF-kB transcriptional activity are known. We identified Ser547 as a new site of p65 phosphorylation targeted by ATM kinase, which coordinates the “DNA Damage Response” pathway in the event of DNA double-strand breaks. We demonstrated that the phosphorylation of Ser547 regulates the transcription of a sub-set of NF-κB dependent genes after genotoxic stress by modifying HDAC1 recruitment(1). Presently, we are investigating the role of this specific phosphorylation in an inflammatory context. We observe that the mutations of p65 (S547A or S547D) also affect the transcriptional potential of the NF-κB in a promoter specific manner after an exposition to TNFα and H2O2. The study of the molecular mechanism of this regulation after TNFα and H2O2 exposition are both in progress. [less ▲]

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See detailDaily intake and bacteriological quality of meat consumed in the households of Kigali city (Rwanda)
Niyonzima, Eugène; Ongol, Martin Patrick; Kimonyo, Anasthase et al

Poster (2015, February 05)

Meat is worldwidely known to be a nutrient rich food. It provides valuable amounts of proteins,vitamins such as retinol and vitamin B12 and minerals namely iron, selenium and zinc with an increased ... [more ▼]

Meat is worldwidely known to be a nutrient rich food. It provides valuable amounts of proteins,vitamins such as retinol and vitamin B12 and minerals namely iron, selenium and zinc with an increased bioavailability than found in other dietary sources [1]. Along the production chain, meat can get contaminated by a wide range of spoilage and/or pathogenic microorganisms from the farm, slaughtering environment and distribution. The actual number of foodborne infections attributable to meat is difficult to assess accurately, principally because only a small proportion of illness cases is officially reported. However, by using outbreak data published internationally, Greig and Ravel [2] reported that 12.7 % of reported foodborne outbreaks were attributable to beef while 10.5 and 4.6 % were associated with chicken and pork, respectively. According to the same authors, Salmonella spp. and pathogenic E.coli, respectively, were identified as the causal agents in 32.9 and 34.6 % of foodborne outbreaks of bacterial origin attributable to beef. The objective of this study was to determine the meat consumption pattern in different socio-conomical categories of the population of Kigali city and to assess the bacteriological quality of the consumed meat. [less ▲]

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See detailSite-specific height-diameter allometry of Central African moist forests
Loubota Panzou, Grâce Jopaul ULg; Doucet, Jean-Louis ULg; Fayolle, Adeline ULg

Poster (2015, February 04)

In this study we aimed to identify the variation in height-diameter allometry between forest types and among species in Central African moist forests. We also examined the consequences on biomass ... [more ▼]

In this study we aimed to identify the variation in height-diameter allometry between forest types and among species in Central African moist forests. We also examined the consequences on biomass estimation. Two forest sites in southern Cameroon with contrasting levels of deciduousness. Height and diameter were measured for a total of 521 trees belonging to 12 timber species over a large range of diameter, 10-240 cm for the Ma’an site and 11-182 cm for the Mindourou site. Non-destructive height measurements were calibrated with destructive measurements for a total of 60 trees, 30 in each site. Commercial forest inventory data (n=7253 0.5ha plots) were gathered for the Ma’an (n=34 samples and 2101 plots) and Mindourou (n=117 samples and 5152 plots) sites. A total of ten allometric models (including asymptotic and non-asymptotic models) were fitted to the height-diameter data at species (n=12) and site (n=2) level. Biomass estimates were computed based on forest inventory data and general allometric models using both site-specific and published height-diameter equations. Given the strong correlation between the non-destructive and destructive height measurements we had confidence in using the non-destructive height measurements to establish site- and species-specific height-diameter allometric equations. The height measurements performed over a wide range of diameters, 10-240 cm, tended to support an asymptotic shape (and most often the Michaelis Menten model) for the height-diameter allometry either at species and site level. We identified a significant difference in height-diameter allometry between the two study sites. For a given diameter, trees tended to be taller in the more semi-deciduous Mindourou site than in the more evergreen Ma’an site, with a maximum height of 39.5 and 46.5 m, respectively. The two sites significantly differed in stand structure and biomass. This difference is due to the variation in height-diameter allometry. Height-diameter allometry strongly varies between sites and site-specific height-diameter allometric equations should be developed to further improve the estimation of biomass and carbon stock contained in tropical forests. [less ▲]

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See detailPrévalence du virus du SDRP dans les élevages porcins
Czaplicki, Guy; Thilmant, Pierre; Van Der Stede, Yves et al

Poster (2015, February)

Porcine reproductive and respiratory syndrome (PRRS) causes major economic losses in pig farms. In different parts of the world, there is growing consensus that PRRS virus (PRRSv) eradication should be ... [more ▼]

Porcine reproductive and respiratory syndrome (PRRS) causes major economic losses in pig farms. In different parts of the world, there is growing consensus that PRRS virus (PRRSv) eradication should be performed on a regional basis, particularly in low density areas of pig production. The objective of the present study was to evaluate the apparent prevalence of PRRSv in Wallonia, a low density area of pig production (0.05 pig farms/km²). A phone survey was addressed to 173 of the 276 Walloon breeding pig owners. One hundred and thirty‐two farmers answered the questionnaire and serological tests were performed in 113 herds. If no vaccination was carried out on sows or on piglets, an Elisa test was performed on 10 breeding and/or 5 feeder pig serum samples collected between November 2011 and December 2012. An individual result was considered negative if the s/p ratio was <0.4; a herd was considered positive if an s/p ratio ≥0.4 was obtained by at least one of its pigs. Globally, at herd level and at animal level, the apparent prevalences were respectively 48% (IC95%: 39 – 57%) and 32 % (IC 95%: 29 – 35%). The present study has shown that half the Walloon breeding pig farms are serologically positive for PRRSv. Such prevalence in an area of low pig production supports the project for a regional PRRS control program. [less ▲]

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See detailRole of the progesterone receptor in the development of sexual behavior in female mice
Desroziers, Elodie ULg; Brock, Olivier; Baum, M.J. et al

Poster (2015, February)

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See detailSingle-Molecule Force Spectroscopy on Synthetic Helical Nanoarchitectures
Devaux, Floriane ULg; Duwez, Anne-Sophie ULg; Huc, Ivan et al

Poster (2015, February)

Foldamers are artificial folded molecular architectures inspired by the structures and functions of natural biopolymers. Folding is the process selected by nature to control the conformation of its ... [more ▼]

Foldamers are artificial folded molecular architectures inspired by the structures and functions of natural biopolymers. Folding is the process selected by nature to control the conformation of its molecular machinery to carry out chemical functions and mechanical tasks, such as en-zyme catalysis, duplication in nucleic acids, force generation,... During the last decade of research on foldamers [1], synthetic oligomers able to adopt well-defined and predictable folded conformations, such as helices, have been proposed. Recent progress has shown that stepwise chemical synthesis and molecular design based on aromatic oligoamide backbones enable to produce large helically folded molecular architectures. The shape and stiffness of the backbone, local conformational preferences, specific interactions between distant monomers in sequences, as well as the action of external parameters such as the solvent or the presence of ions, can be combine to induce folding tendency. A remarkable aspect of these architectures is that they can give rise to folded patterns that have no in natural counterparts biopolymer structures. For instance, helices whose diameter varies along the se-quence, helices possessing a handedness inversion centre, herringbone helices have been reported. The objective of the project is to synthesize various helical nanorchitectures based on an oli-goamide aromatic backbone and to obtain a detailed picture of their dynamical conformation in solution, as well as, their mechanochemical properties, by AFM-based single molecule force spectroscopy. It is worth mentioning that an important sub-objective of this project is to probe intramolecular interactions in small synthetic molecules with the AFM. Indeed, whereas single-molecule force spectroscopy on macromolecules (proteins and synthetic polymers) is widely exploited[2], implementing single-molecule force spectroscopy on small molecules, such as the foldamers proposed here, remains a major challenge[3]. [1] For a review, see G. Guichard and I. Huc, Chem. Commun. 2011, 47, 5933–5941. [2]E. M. Puchner, H. E.Gaub, Curr. Opin. Struct. Biol. 2009, 19, 605–614. [3]P. Lussis, A.-S. Duwez, Nature Nanotech. 2011, 6, 553-557 [less ▲]

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See detailScanning the genome for QTL affecting the recombination process in the male and female cattle germline
Kadri, Naveen Kumar ULg; Harland, Chad ULg; Coppieters, Wouter ULg et al

Poster (2015, February)

We herein study genetic recombination in three dairy cattle population from France, New-Zealand and The Netherlands. We apply a new phasing algorithm extracting familial information suited for large half ... [more ▼]

We herein study genetic recombination in three dairy cattle population from France, New-Zealand and The Netherlands. We apply a new phasing algorithm extracting familial information suited for large half-sib families to reconstruct haplotypes and detect cross-overs. The software is robust to genotyping errors and map errors (genome builts still contain errors for non-model organisms). We identify more than 2,000,000 cross-over events in sperm cells transmitted by 2942 sires to 94,049 offspring, and more than 500,000 cross-over events in oocytes transmitted by 10,943 cows to 23,850 offspring. The estimated number of cross-overs per gamete and its accuracy were influenced by the family structure (number of offsprings, parents and grand-parents genotyped). The average number of cross-overs in males (24.0) was larger than in females (21.8), even after correction for family structure. In males, recombination rates were higher closer to telomeres whereas in females, recombination rates dropped at both centromeres and telomeres (probably as a result of lower informativity). The heritability of the global recombination rate was close to 0.20 in males and to 0.10 in females and the genetic correlation was ~0.70, indicating that common genes are influencing both traits. Genome-wide association studies clearly confirmed QTL located close to REC8 and RNF212 in males. The QTL associated to REC8 was also detected in females and there was a positive correlation between QTL effects in males and females. The QTL associated to REC8 accounted for ~10% of the genetic variance in both males and females. [less ▲]

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See detailNew insights into the fossil record of the mangrove palm Nypa: a taphonomic study from the Iberian Peninsula
Moreno-Dominguez, Rafael; Cascales-Miñana, Borja ULg; Ferrer, Javier et al

Poster (2015, February)

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See detailLordose et/ou xyphose chez le porc : mise à l’épreuve de l’hypothèse héréditaire
Laitat, Martine ULg; Veillat, Emilie; Van Cauwenberge, Henry et al

Poster (2015, February)

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity ... [more ▼]

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity. Pathomorphologically, it may be comparable with Scheuermann’s kyphosis in man and so constitutes a spontaneous model for this humane kyphosis of the thoracic or thoracolumbar spine. In pigs, this condition may decrease the value of carcasses, making deboning efforts challenging. Three major and non‐exclusive hypotheses formulated to explain these back deformations are nutrition, intrauterine viral infection and inherited predisposition. The objective of the present study was to test the latter and, if possible, to identify a locus (some loci) associated with the affection. Forty‐eight pigs were included in this case‐control study. Based on a clinical examination and/or on a measure of the degree of spinal deformity, 25 pigs classified as affected were compared to 23 pigs considered as normal. A whole genome Single Nucleotide Polymorphism (SNP) analysis was performed using a 50,000 SNP array. DNA from forty‐seven samples (tail tissue or blood) was extracted while one sample was eliminated because of its poor quality. After applying quality controls, 40 pigs and 57,838 SNPs (on a total of 62,163) remained for further analysis. One SNP (ASGA0090747) located on Sus scrofa chromosome SSC8 crossed the genome‐wide significant threshold and is thus suspected of being associated with the lordosis and/or kyphosis phenotype. These results seem to confirm the hereditary hypothesis. Further investigations are however needed to confirm the suspected association. [less ▲]

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