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See detailResearch of New Enzyme Producing Strains in the Gut of the Termite Reticulitermes santonensis
Tarayre, Cédric ULg; Bauwens, Julien ULg; Mattéotti, Christel et al

Poster (2013, June)

Termites contain a complex microflora inside of their guts. Inferior termites contain bacteria, mycetes and protists that interact to degrade vegetable components. These strains act as consortia to break ... [more ▼]

Termites contain a complex microflora inside of their guts. Inferior termites contain bacteria, mycetes and protists that interact to degrade vegetable components. These strains act as consortia to break natural materials by secreting various enzymes. Our aim was the isolation and cultivation of microorganisms in order to produce new enzymes that can be further used in green chemistry. Termites were fed with different diets: pinewood, microcristalline cellulose (added with lignin or not), α-cellulose (added with lignin or not) and birchwood xylan. Then, dissections were realized to isolate interesting strains. All the microorganisms were subjected to enzyme assays. That technique allowed us to isolate and to cultivate various strains of bacteria, molds and protists. Three strains of bacteria, two strains of molds and one strain of protist were isolated and displayed different enzymatic activities. The bacteria Bacillus subtilis strain ABGx, Bacillus sp. strain CTGx and Chryseobacterium sp. strain CTGx displayed amylase, cellulase and xylanase activities. The molds Trichoderma virens strain CTGx and Sarocladium kiliense strain CTGx were also able to produce those enzymes. However, the protist Poterioochromonas sp. was found to produce only amylase. In conlusion, the termite gut is a complex culivation medium that provides a habitat for many microorganisms that show interesting enzymatic activities. [less ▲]

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See detailTowards the improvement of a rhizosecretion-based recombinant protein production system: Developing protease-depleted lines of Arabidopsis thaliana.
Lallemand, Jérôme ULg; Désiron, Carole ULg; Périlleux, Claire ULg et al

Poster (2013, June)

Besides traditional production systems, such as bacteria, yeasts and mammal cells, plants can now be used to produce eukaryotic recombinant proteins. Their advantages as hosts for protein production ... [more ▼]

Besides traditional production systems, such as bacteria, yeasts and mammal cells, plants can now be used to produce eukaryotic recombinant proteins. Their advantages as hosts for protein production include correct post-translational modifications, low cost of maintenance and no risk of contamination by human pathogens. Targeting heterologous proteins to the extracellular space is required for the correct folding of complex proteins and makes harvesting and purification easier. However, the quantity and the quality of recombinant proteins have been proved to be reduced by the action of endogenous co-secreted proteases. In this study, we aimed at identifying active root-secreted (rhizosecreted) proteases in the model plant Arabidopsis thaliana. Their activity was assayed by in vitro degradation of a target protein (Bovine Serum Albumine, BSA) in a range of pH. The protease classes involved in BSA degradation were evaluated by inhibitor-based assays that revealed serine proteases as the major class involved in this degradation in any tested conditions. As a first step towards identification, and subsequent silencing, of the most active members of this class, rhizosecreted proteases are being analyzed by the “Activity-Based Protein Profiling” approach. [less ▲]

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See detailThermal adaptation of the ribosomal chaperone trigger factor
Godin, Amandine ULg; Schmidpeter, Phillip; Schmid, Franz et al

Poster (2013, June)

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See detailROBOT-ASSISTED SURGERY: THE MULTICENTER BELGIAN EXPERIENCE
GOFFIN, Frédéric ULg; Traen, koen; TIMMERMANS, Marie ULg et al

Poster (2013, June)

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See detailTo Be Afraid or to Be Proud? The Impact of Emotional Valence and Response Efficacy on Persuasion in Anti-Drink Driving Advertising
Hazee, Simon ULg

Poster (2013, June)

Social marketers traditionally use negative emotions although they can be ineffective on persuasion. However, few social marketers use positive emotions while commercial marketers use them extensively ... [more ▼]

Social marketers traditionally use negative emotions although they can be ineffective on persuasion. However, few social marketers use positive emotions while commercial marketers use them extensively. Accordingly, this study aims to understand the impact of positive versus negative emotional appeals on persuasion of young adults in a road safety context through a 2 (pride versus fear) × 2 (low versus high response efficacy) factorial design. ANCOVA results show the significant role of both types of emotions on persuasion, regardless the level of response efficacy. Surprisingly, “fear” is more persuasive than “pride”. Hence, social marketers should consider using emotions when building future models and communication campaigns targeting young adults. Future research should investigate under which conditions ‘pride’ can be the most persuasive. [less ▲]

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See detailChange in viability of Acetobacter senegalensis cells during gluconic acid fermentation at high temperature
Zarmehrkhorshid, Raziyeh ULg; Shafiei, Rasoul ULg; Thonart, Philippe ULg

Poster (2013, June)

Introduction: Gluconic acid (GA) is a multifunctional carbonic acid with versatile applications in food, beverage and pharmaceutical industries. Although the production of GA and its derivative dating ... [more ▼]

Introduction: Gluconic acid (GA) is a multifunctional carbonic acid with versatile applications in food, beverage and pharmaceutical industries. Although the production of GA and its derivative dating backs decades, but use of this acid and its derivatives due to high prices is currently restricted. Using a thermotolerant bacterium in production of this acid at high temperature can provide a new option for industrially cost effective production. However, fermentation productivity may be negatively affected by factors (such as high temperature) leading to loss of cell viability. Objectives: In this study, the ability of a thermotolerant bacterium, Acetobacter senegalensis, in gluconic acid production at high temperature and its survival responses to some factors including temperature and carbon sources were evaluated. Materials and Method: Different batch fermentation processes were carried out at 38 °C, and then cell viability (total dehydrogenase activity) and culturability were assessed using flow cytometry and plate counting techniques, respectively. Results: A. senegalensis oxidized 95 g/L of glucose to gluconic acid at 38 °C. In exponential growth phase, cells were less subjected to damages; but upon transition of cells to stationary phase, cell viability and culturability reduced. Consequently, due to the lack of dehydrogenase activity the specific rate of glucose consumption and gluconic acid production decreased dramatically. High temperature (38 °C), oxidation of high amount of glucose and accumulation of inhibitory compounds (possibly gluconic acid) were dominant inducers leading cells into a viable but non-culturable state (VBNC) during the course of stationary phase. In contrast, presence of ethanol accompanied with glucose, and low incubation temperature assisted in resuscitation of senescent cells of stationary phase. Conclusions: A. senegalensis is able to produce gluconic acid at 38 °C. But, due to entrance of cells into VBNC state during stationary phase, the performance of batch fermentation is adversely affected. [less ▲]

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See detailThe hidden half of flowering
Bouché, Frédéric ULg; Mistiaen, Kevin ULg; D'Aloia, Maria ULg et al

Poster (2013, June)

Flowering is one of the most important developmental steps in plant life cycle and is therefore tightly controlled by environmental cues. The involvement of the aerial part of the plant in the molecular ... [more ▼]

Flowering is one of the most important developmental steps in plant life cycle and is therefore tightly controlled by environmental cues. The involvement of the aerial part of the plant in the molecular mechanisms leading to floral transition is well documented while participation of the roots received less attention. Nevertheless, the induction of flowering by photoperiod is known to involve systemic signals that move in phloem sap towards sinks, throughout the plants, including the roots. Transcriptomic analysis of roots tissues during the floral induction of flowering by a single long day of in Arabidopsis thaliana by a single long day allowed us to identify a large number of differentially expressed genes. How mutations We subsequently selected in some candidate genes affect plant development - including root architecture and flowering time - is being to analyze their flowering timefurther analyzed. Further analysis of those genes will permit us to unravel their role in the flowering induction process. [less ▲]

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See detailBrain metabolic dysfunction in Capgras syndrome during Alzheimer’s disease: a positron emission tomography study
Jedidi, Haroun ULg; Daury, Noémy; Cappa, Rémi et al

Poster (2013, June)

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See detailMaladie hémolytique néonatale modérée due à un anti‐RH46
MONFORT, Mélanie ULg

Poster (2013, June)

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See detailUnravelling the roles of lysine acetylation by Elp3 during inner ear development
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Laguesse, Sophie ULg et al

Poster (2013, May 31)

Given the importance of acetylation homeostasis in controlling developmental processes, we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a ... [more ▼]

Given the importance of acetylation homeostasis in controlling developmental processes, we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a member of the Elongator complex recently implicated in neurogenesis. We first analysed the spatio-temporal pattern of ELp3 mRNA expression and showed that it was expressed in the early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea, the spiral ganglion, the stria vascularis and the vestibule. To unravel functions of Elp3 in the inner ear, we used conditional knock-out mice in which Elp3 gene is deleted from early otocyst (Elp3 cKO). We submitted these mice to a battery of vestibular testing and found significant abnormalities. Besides, the auditory brain stem response of Elp3 cKO indicated that these mice are severely deaf. At the cellular level, we detected some defaults in the planar orientation of the auditory hair cell bundle. In addition, the length of the kinocilium was significantly reduced both in vestibular and cochlear hair cells from Elp3 cKO mice. We were also able to demonstrate an increased level of apoptosis in the Elp3 cKO spiral ganglion at E14.5 leading to a reduced number of fibers innervating the cochlear hair cells as well as a reduced number of their synaptic ribbons. In conclusion, our results clearly show a role for Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins that are targeted for acetylation by Elp3. [less ▲]

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See detailIn vitro and in vivo Characterization of Adult Bone Marrow Neural Crest Stem Cells
Coste, Cécile ULg; Neirinckx, Virginie ULg; Manguette, Jérôme et al

Poster (2013, May 31)

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See detailNovel phenolic glycolipids: antioxidant activity and effect on membrane models
Sainvitu, Pauline ULg; Nasir, Mehmet Nail ULg; Draguet, Florian et al

Poster (2013, May 30)

Aromatic glycolipids are of both medical as well as pharmaceutical interest. Antimicrobial, antiviraland antiinflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B ... [more ▼]

Aromatic glycolipids are of both medical as well as pharmaceutical interest. Antimicrobial, antiviraland antiinflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as regards to their aromatic group.Theireffect on cell viability when an oxidative stress is induced was tested. In parallel, their interaction with cell models (liposomes) was studied through membrane fusion and permeability experiments. [less ▲]

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See detailUne méthode d’implantation de tours pour favoriser leur insertion dans le tissu urbain
Saint-Pierre, Claire ULg; Becue, Vincent; Diab, Youssef et al

Poster (2013, May 30)

La question de la verticalité est récemment revenue parmi les préoccupations et débats d'experts de la ville. Les tours continuent de faire l'objet de controverses mais la volonté de les utiliser comme ... [more ▼]

La question de la verticalité est récemment revenue parmi les préoccupations et débats d'experts de la ville. Les tours continuent de faire l'objet de controverses mais la volonté de les utiliser comme outil de développement urbain durable demeure. En France, le processus décisionnel de la construction des tours est linéaire et prend en considération relativement tard, les critères nécessaires à une bonne qualité d'insertion urbaine. L'article propose une méthode d'implantation de tours plus globale, permettant d'évaluer l'insertion urbaine d'une tour par rapport à son environnement ainsi que d'accompagner les acteurs impliqués dans leur prise de décision. [less ▲]

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See detailOsmotic dehydration of pomegranate seeds (PUNICA GRANATUM L.)
Bchir, Brahim ULg; Besbes, Souhail; Attia, Hamadi et al

Poster (2013, May 30)

Osmotic dehydration of pomegranate seeds was carried out at different temperatures (30, 40, 50°C) in a 55°Brix solution of sucrose, glucose, and mixture sucrose & glucose (50:50 wt/wt). The most ... [more ▼]

Osmotic dehydration of pomegranate seeds was carried out at different temperatures (30, 40, 50°C) in a 55°Brix solution of sucrose, glucose, and mixture sucrose & glucose (50:50 wt/wt). The most significant changes of water loss and solids gain took place during the first 20 min of dewatering. During this period, seeds water loss was estimated to 46% in sucrose, 37% in glucose and 41% in mix glucose/sucrose solution. The increase of temperature favoured the increase of water loss, weight reduction, solids gain and effective diffusivity. Differential scanning calorimetry data provided complementary information on the mobility changes of water and solute in osmodehydrated pomegranate seeds. The ratio between % frozen water and % unfreezable water decreased from 5 to 0.5 during the process. That involving the presence of very tightly bound water to the sample, which is very difficult to eliminate with this process. It also appeared that glass transition temperature depends on the types of sugar. [less ▲]

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See detailINFUSION OF THIRD-PARTY MESENCHYMAL STEM CELLS (MSC) AFTER KIDNEY AND LIVER TRANSPLANTATION: A PHASE I-II, OPEN-LABEL, CLINICAL STUDY (EudraCT 2011-001822-81 & NCT01429038)
DETRY, Olivier ULg; DELBOUILLE, Marie-Hélène ULg; LECHANTEUR, Chantal ULg et al

Poster (2013, May 30)

MSC cells have demonstrated significant immunosuppressive effects in various in vivo and in vitro studies. This study aims to be the first evaluation of the safety and tolerability of third party MSC ... [more ▼]

MSC cells have demonstrated significant immunosuppressive effects in various in vivo and in vitro studies. This study aims to be the first evaluation of the safety and tolerability of third party MSC infusion after cadaveric kidney and liver transplantation in a prospective phase I-II study, taking advantage of our centre expertise and experience in MSC use in graft-versus-host disease (GVHD) after bone marrow transplantation and using an already functioning GMP-compliant laboratory producing clinical-grade MSC. Secondary end-points will help to evaluate the immunosuppressive potential of MSC after organ transplantation, and the opportunity to develop larger randomised, controlled, phase III trials. After successful transplantation, 10 liver and 10 kidney transplant recipients under standard immunosuppression (tacrolimus, MMF, steroids) will receive an intravenous infusion of 1.5-3x106/kg of third-party MSC on post-operative day 3±2. These patients will be prospectively compared to 10 liver and 10 kidney recipients who meet the inclusion criteria but deny MSC infusion. Safety will be assessed by recording side effects, including opportunistic infections and cancers. Immunosuppressive potential will be evaluated by rejection episode rates, by graft/patient survivals, by immunohistology of 3-months kidney and 6-month liver graft biopsies and by in vitro evaluation of the immunity profile of the recipients. In a second step, reduction (kidney) and progressive weaning (liver) of immunosuppression will be attempted in recipients who received MSC. This ongoing study is supported by research grants from the CHU of Liège, University of Liège, and by the Senior Clinical Research Grant from ESOT. The first patients were included and treated in early 2012, and final results expected in late 2013. [less ▲]

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See detailFunctional and physicochemical properties of starches isolated from pearl millet landraces grown in hyperarid ecosystem.
Boudries, Nadia; Sindic, Marianne ULg; Belhaneche-Bensamra, Naima et al

Poster (2013, May 29)

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See detailNegative valence affects false working memories too
Dehon, Hedwige ULg; Martial, Charlotte ULg

Poster (2013, May 28)

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See detailInteraction between short-term and long-term memory in the musical domain: the impact of musical knowledge and musical expertise
Gorin, Simon ULg; Majerus, Steve ULg

Poster (2013, May 28)

While verbal short-term memory (STM) has received considerable research interest, STM for music has been given considerably less attention. The aim of this study is to show that STM for musical stimuli is ... [more ▼]

While verbal short-term memory (STM) has received considerable research interest, STM for music has been given considerably less attention. The aim of this study is to show that STM for musical stimuli is grounded in LTM, as has been shown for verbal STM. Interactions between LTM and musical STM were studied by exploring the impact of musical knowledge and musical expertise on STM performance. The role of musical knowledge was investigated by an implicit musical learning task, where participants were incidentally exposed to a sequence of tones whose succession was governed by an artificial musical grammar; after exposure, a musical STM task was presented where participants had to reproduce tone sequences of increasing length, half of the sequences being legal (obeying to the artificial musical grammar of the incidental learning task). The role of musical expertise was explored by administering the same task to two participant groups: adults with no musical training and adult musicians. For the role of newly acquired musical knowledge, the non-musician participants showed a significant advantage for reproducing legal musical sequences, showing that they had incidentally learned new musical knowledge and that this knowledge supports STM performance. The musicians did not present an incidental musical learning effect in STM recall, but overall outperformed the non-musicians for reproducing both legal and illegal tone sequences, showing an overall effect of musical expertise. This study is the first to document STM-LTM interactions in the musical domain, and this for both new and existing musical knowledge. [less ▲]

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See detailCombination of actor's play training and assertiveness program to reduce difficulties in self-affirmation: A preliminary study.
Wagener, Aurélie ULg; Delvaux, Muriel ULg; Sarto, Danielle et al

Poster (2013, May 28)

BACKGROUND. Difficulties in assertiveness are often reported by individuals suffering from various psychological problems involving anxiety, depression. Cognitive and behavioral therapeutic procedures ... [more ▼]

BACKGROUND. Difficulties in assertiveness are often reported by individuals suffering from various psychological problems involving anxiety, depression. Cognitive and behavioral therapeutic procedures have already proven their effectiveness in helping these patients. The aim of our study was to test the efficacy of new therapeutic procedure, inspired by the third wave of behavioral and cognitive psychotherapies. METHOD. Our study used an actor’s play training combined with an assertiveness program which comprised ten sessions: five of which were animated by an actress and five by a psychologist-psychotherapist. Each actor play’s session was given alternatively with a psychoeducation’s session. Twenty subjects participated in this program. They fulfilled these pre and post-test assessments: social self-efficacy questionnaire, self-affirmation scales, self-esteem scale, communication scale, anxiety and depression scales. Repeated measures’ ANOVAs have been used to compare pre and post-test results. RESULTS. Results showed statistically significant improvements on each dependent variable, except for the Communication Scale. The higher effects appeared for Beck Depression Inventory and Social Self-efficacy Questionnaire which presented an effect size of 0,74 and 0,56, respectively. Scores on STAI-A and B and on Self-affirmation Scale were over the effect size’s threshold. However, these improvements don’t attain control group’s scores reported in the scientific literature. DISCUSSION. The results highlighted that our program is effective in reducing difficulties in assertiveness. [less ▲]

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See detailThe experience of chronic illness and psychopathology across the life stories: The case of hemophilic boys, obese adolescents and depressive hospitalized adolescents.
Jadin, Aurore ULg; Boulard, Aurore ULg

Poster (2013, May 28)

During an individual semi-structured interview, based on Mc Adams’ wor ks, each teenager’s life story was collected. Different measures were taken. CESD was used to identified depre ssed adolescents among ... [more ▼]

During an individual semi-structured interview, based on Mc Adams’ wor ks, each teenager’s life story was collected. Different measures were taken. CESD was used to identified depre ssed adolescents among sample. In all, we interviewed 66 adolescents. Each personal narrative was then retr anscribed and encoded. Finally, discursive analysis and textual data analysis softwares were used to examine the data base [less ▲]

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See detailPsychosocial needs and perception of inequity: How spouses react to the cancer of their partner?
Wagener, Aurélie ULg; Bragard, Isabelle ULg; Jonius, Bénédicte et al

Poster (2013, May 28)

Background. Several studies have highlighted the importance of psychosocial needs in cancer patients’ spouses. Our study’s aim was to reduce these spouses’ needs, using a combination of two ... [more ▼]

Background. Several studies have highlighted the importance of psychosocial needs in cancer patients’ spouses. Our study’s aim was to reduce these spouses’ needs, using a combination of two psychotherapeutic methods: 1. Psychosocial needs’ organization into a hierarchy; 2. Problem-solving method. We also assessed perception of inequity, which is a new concept used in psycho-oncology. This perception focusses on two feelings that spouses might experience in their relationships: firstly, the feeling of overbenefit and underinvestment; secondly, the feeling of underbenefit and overinvestment. Method. A longitudinal design with three assessments (T0,T1,T2) and two groups (experimental, control) was employed. Spouses fulfilled questionnaires: socio-demographic questionnaire, Psychosocial Needs Inventory, Perception of Inequity, Hospital Anxiety and Depression Scale. At T0, all participants organised their psychosocial needs into a hierarchy. Experimental group practiced problem-solving method before control group. Indeed, experimental group practiced it between T0 and T1 while control group practiced it only between T1 and T2. Results. Thirty-seven spouses participated: N experimental group=19; N control group=18. Whenever the combination was given, repeated measures’ ANOVAs highlighted a significant decrease in unsatisfied psychosocial needs when participants have received both methods. Nevertheless, our results did not replicate previous findings according to which spouses experience a higher feeling of overinvestment and underbenefit than feeling of underinvestment and overbenefit. Discussion. Our results underline the interest of proposing psychotherapeutic methods to cancer patients’ spouses to reduce unsatisfied psychosocial needs. [less ▲]

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See detailCharacterization of the zebrafish beta-cell transcriptome by RNA-seq
Manfroid, Isabelle ULg; Tarifeno, Estefania; Voz, Marianne ULg et al

Poster (2013, May 27)

The loss of pancreatic insulin-producing cells (beta-cells) is a hallmark of diabetes and more knowledge is needed to find new treatments. Thus, it is crucial to identify novel regulatory genes ... [more ▼]

The loss of pancreatic insulin-producing cells (beta-cells) is a hallmark of diabetes and more knowledge is needed to find new treatments. Thus, it is crucial to identify novel regulatory genes specifically expressed in this pancreatic cell subtype. In the present study, the main pancreatic islet was dissected from transgenic Tg(insulin:GFP) adult zebrafish and beta-cells were selectively recovered by FACS with 98% of purity. Illumina RNA-seq was used to sequence the transcriptome. 20 millions of sequenced reads (paired-end) were obtained, aligned on the zebrafish genome and assembled into transcripts (Tophat/Cufflinks softwares). The zebrafish beta-cells transcriptome includes all known regulatory genes involved in beta-cell differentiation such as pdx1, mnx1, pax6b, neuroD, isl1, insm1, as well as Hopx and Hdac9 genes, both recently identified in human beta-cells. In contrast, the alpha-cell specific transcription factor arx and the acinar marker ptf1a were not detected, confirming the high purity of our beta-cell preparation. Interestingly, many miRNAs were detected, such as dre-mir-375 and dre-mir-7, as well as several lncRNA recently described at embryonic stages. We are currently applying the same approach to the Tg(somatostatin:GFP) and Tg(glucagon:GFP) transgenic lines in to characterize the transcriptome of delta- and alpha-cells. The comparison of these different data will allow us to identify coding and non-coding genes specifically expressed in the different endocrine subtype cells, paving the way for further functional studies. [less ▲]

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See detailEnergy spectrum and point spread function comparison of pin-hole and parallel-hole collimators for 90Y bremsstrahlung imaging
Walrand, Stephan; Hesse, Michel; Seret, Alain ULg et al

Poster (2013, May 25)

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See detailLesion size effect on variability in PET quantification in multicenter trials
Guiot, Thomas; Vanderlinden, Bruno; Seret, Alain ULg et al

Poster (2013, May 25)

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See detailDevelopment of a quantitative approach to measure phospholipids in dried drops by Raman spectroscopy
Malherbe, Cédric ULg; Jadoul, Laure ULg; Gilbert, Bernard ULg et al

Poster (2013, May 24)

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass ... [more ▼]

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass spectrometry enables the structural analysis of PL in complex biological media but imaging mass spectrometry by MALDI-MS is rather limited for quantification purposes. Complementarily, Raman spectroscopy as a non invasive and non destructive method is a potential candidate to quantify and visualise the spatial distribution of the PL by molecular imaging. Unfortunately, the lack of specific chemical function in PL, compared to others biomolecules, limits the use of Raman spectroscopy in the identification process of those PL in complex biological samples. The results presented here belong to a first study of the application of the Raman analyses on dried residues of PL and mice brain tissue performed in the lab. [less ▲]

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See detailThe most non-classical symmetric states of an N-qubit system
Baguette, Dorian ULg; Martin, John ULg

Poster (2013, May 23)

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See detailInfluence of dipole-dipole interactions on the superradiant pulse
Damanet, François ULg; Martin, John ULg

Poster (2013, May 23)

Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and ... [more ▼]

Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and single photon cooperative emission [2]. The dissipative dynamics of the atoms is known to depend dramatically on the ratio between the typical inter-atomic distance and the atomic transition wavelength, notably because of dipole-dipole interactions [3]. In this work, we study the effects of these interactions on superradiance as in [4] by solving numerically the corresponding master equation. In particular, by averaging over many realizations of the randomly distributed atomic positions, we show that the decay of the radiated energy pulse height with the intensity of the dipolar coupling follows a power law. [1] E. Ackermans, A. Gero & R. Kaiser, Phys. Rev. Lett. 101, 103602 (2008). [2] R. Friedberg & J. T. Manassah, J. Phys. B 43, 035501 (2010). [3] M. Gross & S. Haroche, Physics reports 93, 301-396 (1982). [4] B. Coffey & R. Friedberg, Phys. Rev. A 17, 1033 (1978). [less ▲]

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See detailAssessment of a 3S rotary atomizer
Ouled Taleb Salah, Sofiene ULg; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

Poster (2013, May 23)

Spinning disc sprayers were first introduced to control cotton pests and diseases. These atomizers were recognized for their production of a uniform droplet size spectrum than conventional nozzles. They ... [more ▼]

Spinning disc sprayers were first introduced to control cotton pests and diseases. These atomizers were recognized for their production of a uniform droplet size spectrum than conventional nozzles. They have proved to be the most successful way of delivering pesticides in the form of Controlled Droplet Application (CDA) at very low volume application. But they were almost abandoned since the years of 1990 for high and medium volume application. Their use was found inefficient in arable crops such as cereals due to the use of inappropriate application rate and problem of penetration of spray in cereal canopies. However, these spray generators may be the best solution when the spray was to be targeted to a small weed with hydrophobic leaf surface where adhesion in the impact is essential for treatment efficiency while minimizing drift and splash thanks to reduced droplet span. In this study, aiming to maximize the control of black grass in cereals, characterization of the droplet size spectra was performed to predict the trajectory droplet and estimate the number that will hit the targeted surface. So, a CDA Micromax Ltd rotary atomizer 3S was operated at different rotation speeds (2000, 3500 and 5000 rpm), flow rates and pressures. A camera X stream -3S which allows the acquisition images in PIV mode, connected to a led lightening set at double mode exposure, were placed in front of the edge of the disc on a spray bench. Images were treated through a laboratory developed Matlab code for Particle Tracking Velocimetry Sizing. Droplets speeds according to diameters were extracted. Droplets cumulative volume according to diameters was also obtained. As the flow rate rose, the volume median diameter (VMD) increased. On the other hand, the VMD decreased as the rotation speed increased. Satellites droplets were observed when the atomizer was operating at a disc speed of 2000 rpm. For each flow rate corresponding to a drop size VMD, the number of droplets/cm2 that will affect pesticide coverage was calculated. Thus, the number of droplets that will effectively adhere depend on their impact at the moment of their arrival to the surface target. [less ▲]

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See detailPrevalence of PRRS virus in Wallonia (Belgium)
Czaplicki, Guy; Thilmant, Pierre ULg; Hooyberghs, Josef et al

Poster (2013, May 22)

Porcine reproductive and respiratory syndrome (PRRS) causes major economical losses in pig farms. In different parts of the world, there is a growing consensus that PRRS virus (PRRSv) eradication should ... [more ▼]

Porcine reproductive and respiratory syndrome (PRRS) causes major economical losses in pig farms. In different parts of the world, there is a growing consensus that PRRS virus (PRRSv) eradication should be performed on a regional basis, particularly in low density areas of pig production. The objective of the present study was to evaluate the apparent prevalence of PRRSv in Wallonia, a low density area of pig production (0.05 pig farms/km²). At this stage of the study, a phone survey has been addressed to 61/276 Walloon breeding pig owners. Fifty-eight answered to the questionnaire: 51 are responsible of a farrow-to-finish farm, 6 of a farrowing farm and 1 of a PRRS-free boar station. In 35% of the tested farms, sows are vaccinated with a modified live virus (MLV) PRRS vaccine (n=11) or with a killed PRRS vaccine (n=9). In two farms, both sows and piglets are vaccinated with a MLV PRRS vaccine. Replacement gilts are purchased in 33% farms. Among the 38 farms raising replacement gilts, 39% purchase boar(s). Boar semen is purchased in 86% of the farms: 56% purchase only boar semen; 30% purchase both semen and boar(s) and 14% of farms only purchase boar(s). In 22/57 tested farms, clinical signs evocative of PRRSv infection were observed over the last 10 years. In 30 farms a PRRS diagnostic test was performed in the past with a positive result in 17. When the pig owner did agree and if no vaccination was carried out on sows or on piglets, a serological Elisa test was performed in 10 breeding pigs and/or 5 feeder pigs >70 kg (or in all available sera if a smaller number of pigs was present). Globally, at herd level and at animal level, the apparent prevalence were respectively 35.2% (95% CI: 23.0-47.4%) and 23.4% (95% CI: 20.1-26.6%). [less ▲]

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See detailA clinical case of congenital tremors in piglets without evidence of PCV-1 and PCV-2
wavreille; Simon, Fanny (Co-first author); Servais, Vincent et al

Poster (2013, May 22)

Congenital tremor (CT) is a disease of newborn pigs characterized by spontaneous clonic contractions of one or more groups of voluntary muscles. Besides suspected or confirmed etiologies of CT such as ... [more ▼]

Congenital tremor (CT) is a disease of newborn pigs characterized by spontaneous clonic contractions of one or more groups of voluntary muscles. Besides suspected or confirmed etiologies of CT such as classical swine fever virus, pseudorabies virus, Japanese encephalomyelitis virus, hereditary disorders in Landrace or Saddleback pigs, organophosphorus poisoning etc., porcine circovirus (PCV) has been described as a potential cause of CT. The type AII seems to be the most common form of CT. Although a potential association between PCV1 or PCV2 and CT-AII has been observed, about 50% CT cases described up till now are caused by unknown reasons. In a PCV-seropositive 108-sow, farrow-to-finish Belgian pig farm breeding hyperprolific Landrace, 42 litters with shaking piglet(s) were reported since June 2006. On March 2012, piglets born from four sows of a 27 sow batch demonstrated CT. After exclusion of main etiologies of CT from these CT-affected piglets, it was hypothesized that PCV1 or PCV2 could be the reason. Necropsies (n=8) and histopathology (n=3) were performed and no evidence of macroscopic or microscopic lesions were seen in cerebrum, cerebellum and spinal cord. Pre-suckled and post-suckled (after 3 days of colostrum uptake) serum samples were also collected from 9 piglets to determine PCV1- and PCV2-specific Ab titres by an immuno-peroxidase monolayer assay (IPMA). No PCV-specific Ab titres were observed in pre-suckled serum samples (≤40), whereas IPMA Ab titres of ≥640 were observed in post-suckled serum samples. Both PCV1 and PCV2 could not be isolated (<101.7 TCID50/g tissue) from 4 tested piglets (in heart, brain and lungs). The present results do not support the hypothesis that PCV1 or PCV2 are linked to CT in newborn piglets. [less ▲]

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See detailApplication of three entomopathogenic fungi for aphid control
Bawin, Thomas ULg; Seye, Fawrou; Boukraa, Slimane ULg et al

Poster (2013, May 21)

Aphids (Homoptera: Aphididae) are sap-sucking insect pests that feed on several plants of agronomical importance. Chemical pesticide application is the most commonly used method for aphid control. Due to ... [more ▼]

Aphids (Homoptera: Aphididae) are sap-sucking insect pests that feed on several plants of agronomical importance. Chemical pesticide application is the most commonly used method for aphid control. Due to rapid developments of resistance to pesticides and environmental pollution, integrated pest management, including biological control was promoted. In this context, entomopathogenic fungi are valuable tools for potential aphid control since various fungal strains are already commercially available. The present study aims to evaluate and compare the insecticidal activity of two Metarhizium and one Aspergillus strains against Acyrthosiphon pisum aphid. Fungi were cultivated on wheat bran media in flasks up to sporulation. The solid media were washed with aqueous solutions containing 0.05% Tween 80 before filtration. The content of conidia was determined by haemocytometer. Doses ranging from 10^3 to 10^7 conidia/ml were then applied on young plants with 20 adult parthenogenetic aphids. Batches were incubated at a 16L/8D photoperiod, 25±2°C and 75-80% relative humidity. Adult mortality was assessed in order to determine LD50, LD90 and LT50 values, and the number of nymphs produced was daily recorded. Five days after treatment, mortality rates ranged from 30 to 91% depending on the fungal strain and tested dose. Corresponding LD50 and LD90 values were 1.23 x 10^3 and 1.34 x 10^7, 3.67 x 10^3 and 9.71 x 10^7, 4.95 x 10^2 and 5.65 x 10^7 conidia/ml for Metarhizium sp., Metarhizium anisopliae and Aspergillus clavatus respectively. At the higher dose, the LT50 were reached within 2, 4 and 3 days respectively, whereas the LT50 were never reached in the controls. By contrast, the intrinsic growth rates were significantly different from uninfected aphids only in the case of A. clavatus with 10^6 and 10^7 conidia/ml doses five days after exposure. In conclusion, these fungal isolates induced A. pisum mortality with a similar impact and A. clavatus infection appeared to alter the adults’ fitness. This suggests that these fungi may be candidates for aphid control. Further investigations should be made in order to assess their host range specificity. Toxic metabolites leading to death have to be identified and their safety towards non-target organisms confirmed. Finally, their persistence in the environment as well as the compatibility with over means of aphid control must be verified in a view of a broad integrated pest management. [less ▲]

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See detailEndothelium-specific expression of the microRNA miR-146a by using the RCAS system
Fontaine, Marie ULg; Halkein, Julie; Tabruyn, Sébastien et al

Poster (2013, May 17)

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See detailMacroporous polyhipes from CO2-in-water emulsion templates stabilized by new sugar-based surfactants
Boyère, Cédric ULg; Favrelle, Audrey; Léonard, Alexandre F. et al

Poster (2013, May 16)

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See detailDust historical record in ombrotrophic peat: The case study of a NW European bog
Allan, Mohammed ULg; Fagel, Nathalie ULg; Le Roux, Gael et al

Poster (2013, May 16)

Ombrotrophic peatlands are unique environmental archives of natural and anthropogenic atmospheric dust deposition because their surface layers are exclusively fed by atmospheric inputs. Lead (Pb) isotopes ... [more ▼]

Ombrotrophic peatlands are unique environmental archives of natural and anthropogenic atmospheric dust deposition because their surface layers are exclusively fed by atmospheric inputs. Lead (Pb) isotopes have been often used to evidence any anthropogenic influence in peat, but they only record trace metal pollution. Another radiogenic isotope, the Neodymium (Nd), could be also use to discriminate the sources of dust in peat bogs. Dust fluxes were investigated over the last 2500 years in the Misten peat bog in Eastern Belgium. Our aims were to use Nd isotope signature to decipher between local and distal dust supplies, the Pb isotopes to trace the antropogenic influences. To address these aims we analyzed REE and lithogenic element analyses, as well as the Nd and Pb isotopes, using HR-ICP-MS and MC-ICP-MS, respectively in peat layers dated by 210Pb and 14C. Changes in REE concentration variations in peat samples are correlated with Ti, Al, and Zr that are lithogenic conservative elements, suggesting that REE are immobile in the studied peat bogs and can be used as tracers of dust deposition. Dust fluxes show pronounced increase at BC300, AD600, 1000AD, 1200AD and from 1700AD, recording either influence of human activities (regional erosion due to forest clearing and soil cultivation activities) or local and regional climate changes. Lead enrichments reveal strong human impact over the last 300 years. Lead isotope signatures are consistent with local and regional contamination by coal combustion and smelting activities. Neodymium isotopes allow to identify three periods characterised by dominant-distal sources (at 320AD, 1000 AD and 1700AD). Those periods are consistent with local wetter intervals as indicated by lower humification degree. Local erosion prevails durier drier (higher humification) intervals (-100AD, 600AD). On a global scale more distal supplies are driven during colder periods, in particular Oort and Maunder minima, confirming the hypothesis that cold climate conditions favor distal supplies. Combining geochemical elementary content and isotope data in ombrotrophic peat allows to decipher between dust flux changes related to human and climate forcing. [less ▲]

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See detailLight-induced Hetero-Diels Alder cycloaddition as a new coupling method to biomolecule radiolabeling
Dammicco, Sylvestre ULg; Luxen, André ULg; Thonon, David et al

Poster (2013, May 16)

The formation of a C-18F bond requires hard conditions which is problematic for the biomolecule radiolabelling. The alternative method which has been developed since a few decades consists in ... [more ▼]

The formation of a C-18F bond requires hard conditions which is problematic for the biomolecule radiolabelling. The alternative method which has been developed since a few decades consists in incorporating the 18F on a prosthetic group and coupling it to the biomolecule. The copper (I)-catalysed 1,2,3-triazole formation involving azides and terminal alkynes is a powerful and rapid method of coupling but present the inconvenient of the employment of cytotoxic reagents. The photoclick conjugation is a promising alternative with no need of catalyst[1]. Recently, a light-induced hetero-Diels Alder cycloaddition involving a 3-(hydroxymethyl)-2-naphthol derivative and an electron-rich olefin has been developed[2]. This reaction seems well adapted for the fast conjugation of radionuclides to biomolecules. Herein we report the synthesis of a [18F]fluoronaphtoquinone derivative as prosthetic group and its reaction with vinyl ethers. [less ▲]

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See detailDesign and development of new polyamides by ring-opening polymerization for high performance composite materials
Tunc, Deniz; Carlotti, Stéphane; Lecomte, Philippe ULg et al

Poster (2013, May 16)

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See detailBiophysical characterization of the interaction of novel aromatic glycolipid surfactants with membrane models.
Sainvitu, Pauline ULg; Nasir, Mehmet Nail ULg; Draguet, Florian et al

Poster (2013, May 15)

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic ... [more ▼]

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as a function of their aromatic group. Their interaction with membranes was studied with monolayer models and was predicted by a computational approach. The relationships between these data and their cytotoxicity and antioxidant properties evaluated on cell cultures are discussed. [less ▲]

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See detailMICROSTRUCTURAL AND PHYSICOCHEMICAL CHARACTERIZATION OF PALYGORSKITE CLAYS FROM MARRAKECH HIGH ATLAS, MOROCCO
El Boudour El Idrissi, Hicham ULg; Knidiri, Abdelouahab; Daoudi, Lahcen et al

Poster (2013, May 09)

The studied palygorskite clay mineral has been collected from several localities of Marrakech High Atlas Tertiary series. These series consist of alternation of marl, dolomite, limestone, gypsum and ... [more ▼]

The studied palygorskite clay mineral has been collected from several localities of Marrakech High Atlas Tertiary series. These series consist of alternation of marl, dolomite, limestone, gypsum and phosphatic beds. Palygorskite currently constitutes more than 90% of the clay fraction of these facies. The aim of this study is to characterise the structure and the physicochemical properties of these fibrous clays. Several technics were performed to characterise these clay sediments: X-ray diffraction (XRD) patterns to study its crystal structure. XRF analysis was performed to study the chemical compositions. Differential thermogravimetric analyses (DTA) were used to study the weight loss due to dehydration and dehydroxylation. The microstructure of these fibrous clays is determined by transmission and scanning electron microscop (TEM/SEM). Physical properties were characterised using the plasticity index of Casagrande, the test for shear strength and the oedometer test. The parameter concerning the adsorption capacity of methylene blue is also determined. The palygorskite clays of the Marrakech High Atlas consist of a silty clay of uniform appearance, greyish in color, and very low organic plastic. The thickness of deposit varies from several meters to several hundred meters. SEM and TEM observations show that palygorskite clay minerals display bundle of long fibres wish occurs as interwoven fibrous mats forming the laminae and as coatings and pore-filling, and pore-bridging cements in dolomite. These fibres are characterized by Mg rich chemical compositions, high crystalline structure, high specific surface area and excellent sorptive capacity. XRD show reflections (110) at 10.5 A, associated with all harmonics. The ATD curves show three endothermic peaks (100-200, 250-300 and 400-500 °C) corresponding to different dehydration process. A fourth exotherm peak often preceded by an endothermic reaction (800-900 °C) reflet the crystallization of new mineral phases including mullite. Palygorskite rich samples exhibit a high plasticity index of about 70. The swelling index generally increases with increasing content of palygorskite. In addition, Palygorskite rich samples exhibit high cohesion and a low friction angle. However, the density of the samples decreases with the increase of the content of palygorskite. [less ▲]

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See detailLES GISEMENTS D'ARGILES DE LA REGION DE MARRAKECH, MAROC
El Boudour El Idrissi, Hicham ULg; Daoudi, Lahcen; Fagel, Nathalie ULg

Poster (2013, May 08)

L'argile est une matière première abondante à la surface de la Terre. En raison de ces propriétés physico-chimiques, elle possède une grande variété d'utilisations. Le Maroc est l'un des plus grands ... [more ▼]

L'argile est une matière première abondante à la surface de la Terre. En raison de ces propriétés physico-chimiques, elle possède une grande variété d'utilisations. Le Maroc est l'un des plus grands consommateurs au monde d'argiles, cependant, le domaine majoritaire d’utilisation d’argiles est celui de la céramique. Dans la région de Marrakech, les argiles sont surtout utilisées dans les domaines de basse classe tels que la céramique traditionnelle et la poterie. Jusqu’à présent très peu d’études sont effectués pour valoriser ces argiles. C’est dans cet objectif que s’inscrit l’étude que nous menons sur les argiles du Haut Atlas de Marrakech ; le présent travail a pour objectif de dresser un inventaire des principaux gisements d’argiles de cette région. Les séries du Primaire (en particulier le Viséen) de la région de Marrakech ne sont pas assez altérées pour exploiter les argiles, tel est le cas de la région de Benhmed qui approvisionne les usines céramiques de la région de Settat. Dans les formations triasiques, malgré l'épaisseur et l'apparence argileuse des séries, seuls quelques gisements d'argiles y sont exploités pour la poterie. Les faciès triasiques, riches en illite, sont en effet silteux et assez pauvres en argiles à l'exception de quelques niveaux très minces. Ces argiles sont utilisées au niveau d’Ourika pour la fabrication d’ustensiles rustiques, pots de terre et objets ornementaux. Ces argiles peuvent également constituées des matières premières pour l’élaboration de briques et de substrats pour le revêtement des murs et des sols. Les argiles des formations jurassiques et crétacées sont assez riches en carbonates, ce qui ne convient pas à leur utilisation dans l’industrie céramique. Les séries du Paléocène et de l’Eocène de toute la région sont riches en argiles fibreuses non exploitées, surtout la palygorskite (argile avec de grandes propriétés physiques), ce qui donne des perspective d’utilisation dans des différents domaines industriels. Le Mioplicoène largement développé au pied du Haut Atlas est surtout connu par sa richesse en smectite, plus particulièrement dans la région de Tassaout. En ce qui concerne les séries quaternaires, certains niveaux d’alluvions sont exploités dans la région pour l’industrie céramique (site potier de Tamsloht, Briqueterie Menara,…). Dans la région de Bouchane, le sol quaternaire est considéré comme la seule source d’argiles et d’ustensiles dans cette zone. Enfin des dépôts actuels sont également exploités (limons des oueds Tensift et N’fis, canal la Rocade) pour la fabrication de briques de murailles ou additionnés à d’autres argiles pour augmenter la consistance des pâtes céramiques. [less ▲]

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See detailThe influence of biogeochemical processes on the pH dynamics in the seasonally hypoxic saline Lake Grevelingen, The Netherlands
Hagens, M; Slomp, C; Meysman, F et al

Poster (2013, May 07)

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See detailOverview of the geophysical data derived from long-term FTIR monitoring activities at the Jungfraujoch NDACC site (46.5ºN) and the PYGCHEM project
Mahieu, Emmanuel ULg; Bovy, Benoît ULg; Bader, Whitney ULg et al

Poster (2013, May 07)

We present an overview of the geophysical data deduced from long-term monitoring activities conducted at the Jungfraujoch station by the University of Liège. Typical results and trend investigations are ... [more ▼]

We present an overview of the geophysical data deduced from long-term monitoring activities conducted at the Jungfraujoch station by the University of Liège. Typical results and trend investigations are presented for hydrogen chloride (HCl) and carbonyl sulfide (OCS). We further display and briefly describe time series for new target gases, namely methanol (CH3OH) and HCFC-142b. We also show some preliminary results for ammonia (NH3) and peroxyacetyl nitrate (PAN). Finally, we present the PyGChem project, a Python interface to the GEOS-Chem model currently under development at ULg. [less ▲]

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See detailAnatase TiO2 colloidal nanocrystal-based architectures: models to assess the role of porosity and NCs morphology in Li battery electrode
Krins, Natacha ULg; Buonsanti, Raffaella; Kim, Chunjoong et al

Poster (2013, May 03)

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See detailLuminescence dating of the Palaeolithic site of Kulbulak (Uzbekistan) using IR50 and pIRIR290 signals from K-feldspar
Vandenberghe, Dimitri; Van Nieuland, J.; De Dapper, Morgan et al

Poster (2013, May)

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See detailEtude tribologique sur des lubrifants solides à haute température
Legros, Arnaud ULg; Zaïdi, Hamid; Diny, Mouad et al

Poster (2013, May)

Actuellement, environ 2/3 de l’énergie libérée par la combustion de l’essence est rejetée sous forme de chaleur, soit dans les gaz d’échappement, soit dans le liquide de refroidissement. Un cycle de ... [more ▼]

Actuellement, environ 2/3 de l’énergie libérée par la combustion de l’essence est rejetée sous forme de chaleur, soit dans les gaz d’échappement, soit dans le liquide de refroidissement. Un cycle de Rankine peut être utilisé afin de valoriser la chaleur des gaz d’échappement. Cependant, aucune machine de détente commerciale n’est adaptée aux conditions de récupération d’énergie sur gaz d’échappement. La tribologie, plus particulièrement celle des lubrifiants solides à haute température, peut intervenir afin d’étudier les couples de matériaux adéquats pour réaliser les joints de la machine de détente du cycle. Cela permet de réduire les pertes globales de la machine et d’en améliorer le rendement. [less ▲]

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See detailYouth leaders’ representations about the role of youth organizations in physical activity promotion
Cloes, Marc ULg; Wilkin, Sophie

Poster (2013, May)

Purpose. Multisectorial actions are needed to fight against sedentariness. Youth organizations (YO) gather thousands of children and adolescents each weekend. Most of them propose to develop all ... [more ▼]

Purpose. Multisectorial actions are needed to fight against sedentariness. Youth organizations (YO) gather thousands of children and adolescents each weekend. Most of them propose to develop all dimensions of the individuals and to contribute to the education of the youth. Unfortunately, very few studies are available to confirm if this is the case. The purpose of this study was to determine if the youth leaders consider that they play a role in physical activity (PA) promotion. Methods. A questionnaire was developed and tested by a pilot study. It comprised four parts (Introduction, Descriptive variables; Youth leader’s representations about PA; Youth leader’s representations about the role of the YO in PA promotion). Most of the questions were Lickert scales. Answers of 232 youth leaders were analysed. They were belonging to all youth movements of the Liege area. Results. Youth leaders tended to support the idea that YO have to play a role in PA promotion: 57.3% totally agree on the fact that YO allows youth to be physically active; 40.1% consider that it is effectively the case. On the other hand, only 1.3% believes that their group attend to sports during YO’s activities and 4.7% that youth use what they learn about PA in YO in their everyday life. Conclusions This study emphasized that YO should be more involved as an determining actor in the overall PA promotion and that youth leaders should be more systematically informed and prepared about the potential role that they could play. [less ▲]

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See detailThermal adaptation of the ribosomal chaperone trigger factor
Godin, Amandine ULg; Schmidpeter, Phillip; Schmid, Franz et al

Poster (2013, May)

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See detailBiogeochemistry and carbon mass balance of a coccolithophore bloom in the northern Bay of Biscay (June 2006)
Harlay, Jérôme ULg; Chou, Lei; Van Oostende, Nicolas et al

Poster (2013, May)

Primary production (PP), calcification (CAL), bacterial production (BP) and dark community respiration (DCR) were measured along with a set of various biogeochemical variables, in early June 2006, at ... [more ▼]

Primary production (PP), calcification (CAL), bacterial production (BP) and dark community respiration (DCR) were measured along with a set of various biogeochemical variables, in early June 2006, at several stations at the shelf break of the northern Bay of Biscay. The cruise was carried out after the main spring diatom bloom that, based on the analysis of a time-series of remotely sensed chlorophyll-a (Chl-a), peaked in mid-April. Remotely sensed sea surface temperature (SST) indicated the occurrence of enhanced vertical mixing (due to internal tides) at the continental slope, while adjacent waters on the continental shelf were stratified, as confirmed by vertical profiles of temperature acquired during the cruise. The surface layer of the stratified water masses (on the continental shelf) was depleted of inorganic nutrients. Dissolved silicate (DSi) levels probably did not allow significant diatom development. We hypothesize that mixing at the continental slope allowed the injection of inorganic nutrients that triggered the blooming of mixed phytoplanktonic communities dominated by coccolithophores (Emiliania huxleyi) that were favoured with regards to diatoms due to the low DSi levels. Based on this conceptual frame, we used an indicator of vertical stratification to classify the different sampled stations, and to reconstruct the possible evolution of the bloom from the onset at the continental slope (triggered by vertical mixing) through its development as the water mass was advected on-shelf and stratified. We also established a carbon mass balance at each station by integrating in the photic layer PP, CAL and DCR. This allowed computation at each station of the contribution of PP, CAL and DCR to CO2 fluxes in the photic layer, and how they changed from one station to another along the sequence of bloom development (as traced by the stratification indicator). This also showed a shift from net autotrophy to net heterotrophy as the water mass aged (stratified), and suggested the importance of extracellular production of carbon to sustain the bacterial demand in the photic and aphotic layers. [less ▲]

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See detailQuality controls of no-carrier-added aromatic amino acids such as FDOPA and FTYR produced at curie level
Libert, Lionel ULg; Lemaire, Christian ULg; Giacomelli, Fabrice ULg et al

Poster (2013, May)

Aromatic fluoro amino acids such as 2-[18F]fluoro-L-tyrosine (FTYR) and 6-[18F]fluoro-L-DOPA (FDOPA) are useful radiopharmaceuticals for oncologic studies and evaluation of the presynaptic dopaminergic ... [more ▼]

Aromatic fluoro amino acids such as 2-[18F]fluoro-L-tyrosine (FTYR) and 6-[18F]fluoro-L-DOPA (FDOPA) are useful radiopharmaceuticals for oncologic studies and evaluation of the presynaptic dopaminergic function using positron emission tomography. Recently, a no-carrier-added (nca) enantioselective synthesis of these compounds, based on an multistep PTC approach was automated in a FASTlabTM module from GE . From 185 GBq of [18F]fluoride and after 1 hour of synthesis, more than 37 GBq of FTYR or FDOPA are available . This automated production yields enough doses for many PET studies. A monograph for FDOPA prepared by electrophilic substitution exists , but it is not adapted to the nca nucleophilic synthesis of FDOPA and FTYR, as in this case specific activity, by products and possible impurities are different. A complete quality control (QC) has then be developed in accordance with the guidelines of the European Pharmacopeia (Eur. Ph.). [less ▲]

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See detailHuman papillomavirus entry into NK cells requires CD16 expression and triggers cytotoxic activity and cytokine secretion
Renoux, Virginie; Bisig, Bettina; Langers, Inge ULg et al

Poster (2013, May)

Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV ... [more ▼]

Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV-infected women clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has evaluated the direct interaction between HPVs and NK cells, a key player in host resistance to viruses and tumors. We demonstrated an NK-cell infiltration in HPV- associated preneoplastic cervical lesions. Since HPVs cannot grow in vitro, virus-like particles (VLPs) were used as a model for studying the NK-cell response against the virus. Interestingly, NK cells displayed higher cytotoxic activity and cytokine production (TNF-a and IFN-g) in the presence of HPV-VLPs. Using flow cytometry and microscopy, we observed that NK-cell stimulation was linked to rapid VLP entry into these cells by macropinocytosis. Using CD16+ and CD16- NK-cell lines and a CD16-blocking antibody, we demonstrated that CD16 is necessary for HPV–VLP internalization, as well as for degranulation and cytokine production. Thus, we show for the first time that NK cells interact with HPVs and can participate in the immune response against HPV-induced lesions. [less ▲]

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See detailAnalysis of mainstream tobacco smoke by SPME-GC×GC-TOFMS
Brokl, Michal ULg; Bishop, L; Wright, C et al

Poster (2013, May)

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See detailInvestigation on human cadaveric VOC by TD-GCxGC-TOFMS
Stefanuto, Pierre-Hugues ULg; Stadler, Sonja; Pesesse, Romain et al

Poster (2013, May)

The study of the ‘smell of death’ is a challenging task. Analytical chemists who try to understand human decomposition are facing very complex mixtures of analytes present at various levels. For the last ... [more ▼]

The study of the ‘smell of death’ is a challenging task. Analytical chemists who try to understand human decomposition are facing very complex mixtures of analytes present at various levels. For the last few decades, investigations have been conducted to better learn the process of body decomposition by mean of the measurement of the Volatile Organic Compounds (VOCs) released during decay1. However, the chemical profile of the decomposition odor is still far from being elucidated. Indeed, the complexity of the VOC mixtures makes this profiling difficult to be carried out by a classical gas chromatography mass spectrometry (GC-MS) approach. In previous studies, we developed direct-sampling based approaches for cadaveric VOC analysis from grave soils and decaying bodies by mean of thermal desorption (TD) coupled to comprehensive two-dimensional GC coupled to time-of-flight MS (GC×GC-TOFMS)2,3,4. They were based on the use of human analogs (Sus domesticus L. carcasses). For the present study, we investigated the VOC profile of early stage decomposition of human bodies. We analyzed samples collected during different trials organized during different seasons in a body farm located in Texas. Samples included environmental controls, pig carcasses, and human bodies (protected or not from scavenger insects). The data processing was performed in the light of identifying possible seasonal and species variations. Both peak capacity enhancement and spectral deconvolution helped to characterize VOC mixtures and improve comparisons of profiles. Acknowledgements: Human samples were collected during the project “Development and validation of standard operating procedures for measuring microbial populations for estimating a post-mortem interval” Grant Award No. 2010-DN-BX-K243. The authors of that grant are acknowledged for giving us the opportunity to access cadavers for sampling. 1. Vass, A.A. et al. Decompositional odor analysis database. J Forensic Sci (2004) 49, 1–10. 2. Brasseur C. et al. Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the forensic study of cadaveric volatile organic compounds released in soil by buried decaying pig carcasses. J Chromatogr A (2012) 1255, 163–170. 3. Dekeirsschieter, J. et al. Enhanced characterization of the smell of death by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS). PLoS ONE (2012) 7, e39005. 4. Stadler, S. Characterization of volatile organic compounds from human analogue decomposition using thermal desorption coupled to comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. Anal Chem (2013) 85, 998–1005. [less ▲]

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See detailTowards a Standards-Based Cloud Service Manager
Ghrab, Amine; Skhiri, Sabri; Koener, Hervé et al

Poster (2013, May)

Migrating services to the cloud brings all the benefits of elasticity, scalability and cost-cutting. However, migrating services among different cloud infrastructures or outside of the cloud is not an ... [more ▼]

Migrating services to the cloud brings all the benefits of elasticity, scalability and cost-cutting. However, migrating services among different cloud infrastructures or outside of the cloud is not an obvious task. In addition, distributing services among multiple cloud providers, or on a hybrid installation requires a custom implementation effort that must be repeated at each infrastructure change. This situation raises the lock-in problem and discourages cloud adoption. Cloud computing open standards were designed to face this situation and to bring interoperability and portability to cloud environments. However, they target isolated resources, and do not take into account the notion of complete services. In this paper, we introduce an extension to OCCI, a cloud computing open standard, in order to support complete service definition and management automation. We support this proposal with an open-source framework for service management through compliant cloud infrastructures. [less ▲]

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See detailUser-friendly method for GCxGC optimization
Stefanuto, Pierre-Hugues ULg; Dimandja, Jean-Marie; Focant, Jean-François ULg

Poster (2013, May)

Almost 30 years are gone since the first paper about multidimensional GC was published by John B. Phillips 1. After several years of developing process, Comprehensive Two Dimensional Gas Chromatography ... [more ▼]

Almost 30 years are gone since the first paper about multidimensional GC was published by John B. Phillips 1. After several years of developing process, Comprehensive Two Dimensional Gas Chromatography systems are in the commercialization step of its live time. During this period, the advantages of GC×GC, regarding to classical D1 system, were clearly demonstrated 2. However, the new parameters involve in a GC×GC method are still not completely understand and people don’t use it at the maximum of its capacity. According to the literature more and more group are using GC×GC for different kind of applications. In most of these researches, GC×GC was used for its separation power. Unfortunately, the orthogonality and the column set are most of the time poorly optimized. To help people in this important step, we develop an user-friendly method to choose the best column combination and the best separation parameters for a particular application. All these developments were based on different mix of standard call the Century mix and the Dimandja mix. These are the descendants of the Phillips mix create in 2003 by J. Dimandja 3. These mixtures contain homologous series of compounds representing the polarity and the volatility range commonly sees in GC×GC. This method is based on the Retention Index obtained in a classical GC analysis and projected in the 2D space. Using this projection method, we identified four major types of orthogonality based on the peak dispersion obtained. We name those: Normal, Reverse, Hybrid and Transpose orthogonality. To characterize these observations, we developed the Orthogonality Index. Going back to the mathematical definition of orthogonality, this factor is the angle formed between the alkane line and the aromatic hydrocarbon line in the chromatographic space. Using this predictive tool, people should be able to choose the best column set and to optimize easily the separation parameters. 1. Phillips, J.B., Luu, D., Pawliszyn, J.B. & Carle, G.C. Multiplex gas chromatography by thermal modulation of a fused silica capillary column. Anal. Chem. 57, 2779–2787 (1985). 2. Dimandja, J.-M.D. Comprehensive 2-D GC provides high-performance separations in terms of selectivity, sensitivity, speed, and structure. Anal. Chem. 76, 167A–174A (2004). 3. Dimandja, J., Clouden, G. & Colón, I. Standardized test mixture for the characterization of comprehensive two-dimensional gas chromatography columns: the Phillips mix. J Chromatogr a 1019, 261–272 (2003). [less ▲]

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See detailThe contribution of Multidimensional IRT for modeling applicants performance on a SJT.
Lothe, Benoit ULg; Monseur, Christian ULg; Hansez, Isabelle ULg

Poster (2013, May)

Situational judgment tests (SJTs) are testing methods often used in Personnel Selection to appraise different constructs (Whetzel & McDaniel, 2009). In the framework of an overall career progression ... [more ▼]

Situational judgment tests (SJTs) are testing methods often used in Personnel Selection to appraise different constructs (Whetzel & McDaniel, 2009). In the framework of an overall career progression program in a Public Administration, SJTs were developed to assess several competencies relevant to both task (e.g. planning, managing, etc.) and contextual performance (e.g. helping behaviour, organizational loyalty, etc.). Each competency is a combination of KSAOs needed to perform efficiently in a specific context (Campion & al., 2012; Le Boterf 2008). Given the lack of internal consistency of SJT and the dichotomous nature of the data, we decide to use Multidimensional Item Response Theory (MIRT) model to examine the issue of construct validity. Based on Hartig & Höhler (2009), we use MIRT compensatory model to estimate the latent covariance structures of the SJT. Different model are tested and compared depending on the number of dimension involved, the nature of the items and latent variables relation (between or within-item multidimensionality) and the number of model parameter (one or two parameter model). The results and findings lead us to discuss some implications (gaining accuracy, modeling performance in complex task, etc.) and limitations (dealing with complex loading structure, etc.) of using IRT, over and above classical test theory, in the specific case of SJTs applicants performance. The originality of this communication also lies in the way we addressed SJTs reliability and construct validity issues with a measurement based model that differs from the one usually used in the field. [less ▲]

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See detailWhat are the barriers encountered by the physiotherapists in their role of physical activity promoters?
Cloes, Marc ULg; Fabre, Florian

Poster (2013, May)

Purpose. The fight against sedentariness needs multisectorial actions. Being experts in movement and exercise, physiotherapists (PT) consider themselves as ideally placed to promote, guide and prescribe ... [more ▼]

Purpose. The fight against sedentariness needs multisectorial actions. Being experts in movement and exercise, physiotherapists (PT) consider themselves as ideally placed to promote, guide and prescribe physical activity (PA). The purpose of this study was to identify the barriers that limit their PA promoting mission as well as proposals of solutions. Methods. Fifteen PT attended to a 3 hours meeting during which researchers applied the nominal group technique. After a short introduction summarizing basic notions about PA, subjects were invited to describe the problems that PTs encountered when they want to promote PA. In a second step, they selected 8 categories of barriers to be discussed. During the last part of the meeting, subgroups had to imagine solutions to deal with selected problems. Oral reports were shared. After the meeting, a written report was prepared by the researchers and validated by the subjects. Results. Out of the 13 identified categories, subject selected the following: Patients’ lack of awareness of PA’s interest for healthy living; Patients’ poor self perceptions and misrepresentations about PA; Patients’ difficulties to change their behaviour; PTs’ lack of knowledge about PA; PTs’ lack of informational resources; Patients’ lack of social support; Patients’ lack of facilities; PTs’ lack of interaction with other actors. The synthesis of the solutions was combined with written resources in a 19 pages brochure. Conclusions. This study pointed out that PTs are able to propose a plurality of ideas and approaches through a constructive group work. Such approach will be proposed in inservice education. [less ▲]

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See detailSelection of a spatial hypothesis in geographic profiling using graph theory
Trotta, Marie ULg

Poster (2013, May)

Geographic profiling is based on the spatial hypothesis of a distance decay effect around the offender’s anchor point and can be very useful for delineating prior search areas for individual ... [more ▼]

Geographic profiling is based on the spatial hypothesis of a distance decay effect around the offender’s anchor point and can be very useful for delineating prior search areas for individual investigations such as DNA testing. However, this spatial hypothesis fails to model a significant part of Belgian serial sexual offenders. In previous study, a complementary hypothesis, the minimisation of the variance (MOV) for the distances between the offender’s anchor point and the crime site, exploiting recent literature observation, is proposed to model unexplained behaviours. A systematic comparison of the distance decay and the MOV hypotheses show that their overall capacities to provide an effective geoprofile are similar, but their performances may differ, depending on the geometric pattern of crimes. Here, we use graph theory to describe the favourable patterns for the MOV, in order to select which spatial hypothesis is best suited for a new investigation. While star graphs represent patterns for which both hypotheses could be validated, wheels with preferred directions are only explained by the MOV hypothesis. Real patterns on the road network are a-posteriori evaluated thanks to the generation of the shortest path between the best solution of the MOV and all the crime locations thanks to the Dijkstra algorithm. [less ▲]

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See detailNON-ANTHOCYANIN POLYPHENOLS QUANTIFICATION IN EUTERPE OLERACEA FRUITS BY A UHPLC−LTQ-ORBITRAP MS METHOD
Dias, Aecio; Rozet, Eric ULg; Chataigné, G et al

Poster (2013, May)

High antioxidant and anti-inflammatory activities have been observed from non-anthocyanin polyphenols of E. oleracea fruits [1-2]. The aim of this work was to quantify major non-anthocyanin polyphenols by ... [more ▼]

High antioxidant and anti-inflammatory activities have been observed from non-anthocyanin polyphenols of E. oleracea fruits [1-2]. The aim of this work was to quantify major non-anthocyanin polyphenols by an accurate UHPLC−LTQ-Orbitrap MS method. Fruits were harvested in Pará state (Brazil), processed to pulp and lyophilised. 0.5g of dry pulp powder was defatted by sonication with petroleum ether. The residue was then extracted five times with 5mL MeOH each time for 30 min (optimized conditions giving recovery rates > 90%). The extract was evaporated to dryness with a RapidVap® evaporator at 35°C. Solubilization of the dried extract was realised using 40% MeOH. For the UHPLC quantification, a HSS C18 column (1.8µm) was used with a gradient elution of MeOH and H2O both with 0.1% HCOOH and the ionisation source (ESI) was operated in NI mode. 26 compounds were identified, among them 7 identified for the first time in this fruit. Total error and accuracy profiles were used as validation criteria. Calibration in the matrix was found to be more accurate than calibration without matrix. Trueness, repeatability, intermediate precision, selectivity, response function, linearity and LOD/LOQ for 12 non-anthocyanin phenolic compounds were evaluated and the quantification method validated. [1] J. Kang et al., Food Chem. 122 (2010) 610–617. [2] J. Kang et al., Food Chem. 128 (2011) 152–157. [less ▲]

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See detailStudy on the sensitivity of three oat varieties to the saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae)
Censier, Florence ULg; Chavalle, Sandrine; San Martin y Gomez, Gilles et al

Poster (2013, May)

Saddle gall midge, Haplodiplosis marginata (von Roser) is a univoltine pest of cereals which occurs in Europe. The larvae overwinter in the soil. During the spring, the new emerged females lay eggs on the ... [more ▼]

Saddle gall midge, Haplodiplosis marginata (von Roser) is a univoltine pest of cereals which occurs in Europe. The larvae overwinter in the soil. During the spring, the new emerged females lay eggs on the leaves of cereals and grasses. After hatching, larvae migrate under the leaf sheath, where they develop at the expense of the plant. As a reaction, stems induce saddle-shaped galls of 5 to 10 mm long. Numerous galls can lead to stem breaks and important yield losses when they are numerous. After 40 years without any reporting, large populations of H. marginata and important damage have been observed since 2010 in wheat crops in Belgium, especially in the Flemish Polders where clay soils and intensive farming of cereals favour heavy infestations. According to some research conducted in the 60s during the last outbreak, oat (Avena sativa L.) is known to be one of the less attractive hosts to the saddle gall midge. Our study was thus performed in order to assess the host sensitivity of three oat varieties currently grown in Belgium (Evita, Effektiv and Freddy). Therefore, oat varieties were sown on infested ground in two separate enclosures in a glasshouse. In the first enclosure, only the three oat varieties were grown ; in the second one, these three oat varieties were grown together with two varieties of spring wheat (Granny and KWS Chamsin). Two parameters were measured: the percentage of leaves with laid eggs, and the number of galls per stem. The percentage of leaves with eggs showed that the infestation is significantly lower on oats when they are in presence of wheat. The infestation was also significantly higher on wheat than on oat, which means oat is a much less favoured host plant than spring wheat for laying. Oat varieties were significantly different regarding the number of galls per stem, but with very little damage compared to wheat. The Freddy variety even seemed to be completely resistant to saddle gall midge, as no galls were observed although there were a similar percentage of leaves with eggs for the three oat varieties. Cropping oat could thus contribute to reduce or even to eliminate infestations of H. marginata. [less ▲]

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See detailWhat is proposed to overweight and obese youth in a sample of fitness centres?
Cloes, Marc ULg; LEBRETHON, Marie-Christine ULg; Fortemps, Grégory

Poster (2013, May)

Purpose. Overweight and obesity take a growing place in the public health preoccupations as the proportion of people over the normal BMI limit increases dramatically year after year. This evolution could ... [more ▼]

Purpose. Overweight and obesity take a growing place in the public health preoccupations as the proportion of people over the normal BMI limit increases dramatically year after year. This evolution could have devastating consequences on the economy for years and possibly generations to come. Youth physical activity is pointed out as a priority. Fitness centres are possible partners of health services. This study aimed to know if overweight/obese youth could be welcomed into a sample of fitness centres. Methods. A sample of 13 fitness centres of the Liege area was visited. Fourteen interviews with the managers have been conducted. Specific questions were asked about what is proposed to overweight and obese people and specifically to youth group. Content analysis has been done from verbatim transcripts. Results. Most of the subjects consider that overweight/obese youth could encounter some difficulties to adhere to a fitness centre. Unfounded embarrassment about the look of others is underlined by 7 out of the managers. The real intrinsic motivation of the youth would be a key factor as well as real difficulties to use the equipment. Few fitness centres currently propose specific activities but 7 would be ready to collaborate with a medical unit in order to propose specific activities. Two decided to stop such initiatives according to the cost and lack of perseverance of the youth. Conclusions. This study showed that fitness centres managers would be interested to implement initiatives to offer specific services to overweight/obese youth but it would require collaboration with health services. [less ▲]

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See detailKnowledge about physical activity among older adults: Short term effects of a neighbourhood environment internet-based intervention
Mouton, Alexandre ULg; Rompen, Jérôme ULg; Cloes, Marc ULg

Poster (2013, May)

Purpose. Despite the numerous health benefits, population physical activity levels are low and decline with age. Few studies exposed that a better knowledge about physical activity could encourage people ... [more ▼]

Purpose. Despite the numerous health benefits, population physical activity levels are low and decline with age. Few studies exposed that a better knowledge about physical activity could encourage people to become physically active, which constitute a critical health challenge for older people. Moreover, neighbourhood environment internet-based interventions are recommended to promote physical activity. Therefore, the main purpose of this study was to explore the effects of a neighbourhood environment internet-based intervention on knowledge about physical activity among older adults. Methods. At baseline, 87 older adults (60.69 ± 7.85 years) from the same district were assigned to an intervention (n = 41) or a control (n = 46) group. The two months intervention was composed of a comprehensive physical activity promotion website, with an emphasis on neighbourhood environment contents, and a monthly personalized email based on the stage of change theory. Physical activity knowledge was assessed by questionnaires at baseline, after one and two month of the intervention, and after one month follow-up. Results. After the intervention, knowledge of the physical activity recommendations significantly improved in the intervention group (p = 0.032), while the control group remained unchanged. Besides, knowledge about environmental opportunities for physical activity increased slightly only in the intervention group. Conclusions. These findings suggest that a neighbourhood environment internet-based intervention can result in modest improvements of the physical activity knowledge in older adults. Further research should focus on alternative ways to improve the knowledge about local opportunities for physical activity and its relationship with long term effects on behaviour change. [less ▲]

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