References of "Poster"
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See detailScan for selective sweeps associated with muscular devolpment in Belgian Blue beef cattle
Druet, Tom ULg; Ahariz, Naïma ULg; Cambisano, Nadine ULg et al

Poster (2013, February)

The Belgian Blue beef cattle is well known for its double muscling phenotype resulting from fixation of a deletion in the myostatin gene. Since this fixation, further intensive selection for muscular ... [more ▼]

The Belgian Blue beef cattle is well known for its double muscling phenotype resulting from fixation of a deletion in the myostatin gene. Since this fixation, further intensive selection for muscular development has been particularly succesful. This response to selection might be due to fixation of more genetic variants increasing muscular development. In the present study, we search for selective sweeps in the Belgian Blue genome which might result from the fixation of such variants. To that end we used data from 593 sires genotyped with the BovineHD SNP array. In addition, we used the Belgian Blue dual purpose and the Holstein breeds as controls. We first performed scans for regions of complete homozygosity resulting from fixation. Large fixed regions were found around major genes known to be fixed in the Belgian Blue cattle breed (MSTN, PLAG1 and MC1R) but no other regions of the same magnitude was found. Among the smaller identified regions, only few of them were unique to the Belgian Blue breed. Statistical tests based on long range haplotype information were also implemented, revealing large regions in the genome of reduced haplotype homozygosity specific to the Belgian Blue breed. Some of these regions corresponded to known major genes (MSTN, roan locus, PLAG1 or MC1R) while other regions were new. To determine whether these regions might be the result of selection for muscular development, we performed association studies for muscular development. None of the identified QTL matched with the regions of reduced haplotype homozygosity and the largest QTLs did not presented evidence of strong selective sweeps. These results suggest that the response to selection for muscular development in Belgian Blue beef cattle is probably the result of polygenic selection. [less ▲]

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See detailModeling the mutation of socio-ecosystems in Central Africa
Gillet, Pauline ULg; Vermeulen, Cédric ULg

Poster (2013, February)

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See detailPrevalentie van eenzaamheid bij 65-plussers in België
Vermeulen, Bram; Maggi, Patrick ULg; Delye, Samuel ULg et al

Poster (2013, February)

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See detailComparaison of the allergenicity of the zymogen and mature form of Der p 3.
Bouaziz, Ahlem ULg

Poster (2013, February)

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See detailDifferentiation of Boettcher's Cells During Postnatal Development of Rat Cochlea
Cloes, Marie ULg; Renson, Thomas; Johnen, Nicolas ULg et al

Poster (2013, January 28)

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See detailRole of Gamma Delta T cells in HPV-induced Cancer Progression
Van hede, Dorien ULg; Bastin, Renaud; Francis, Floriane et al

Poster (2013, January 28)

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See detailUnravelling the roles of lysine acetylation by Elp3 during inner ear development
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Laguesse, Sophie ULg et al

Poster (2013, January 28)

The inner ear is composed of the vestibular system that controls balance, and the cochlea, which is dedicated to hearing. In both parts of the inner ear, sensory epithelia comprise supporting cells ... [more ▼]

The inner ear is composed of the vestibular system that controls balance, and the cochlea, which is dedicated to hearing. In both parts of the inner ear, sensory epithelia comprise supporting cells surrounding the sensory hair cells. These cells bear at their apical surface a staircase-structured bundle, consisting of multiple rows of actin-based stereocilia and a single tubulin-based kinocilium. This hair bundle allows the transduction from mechanical stimuli, initiated by sound or gravitational changes, to electrical signals that will then be transmitted by neurons from the spiral ganglion (innervating hair cells of the cochlea) or the vestibular ganglion. The inner ear organogenesis requires a tightly regulated transcriptional program that can be affected by post-transcriptional and post-translational modifications among which lysine acetylation. Given the importance of acetylation homeostasis in controlling developmental processes, we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a member of the Elongator complex recently implicated in neurogenesis. To determine the role of Elp3 in the inner ear, we first determine the spatio-temporal pattern of ELp3 mRNA expression and showed that it was expressed in the entire early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea (the organ of Corti), in the spiral ganglion, in the stria vascularis and in the vestibule. To unravel in vivo functions of Elp3 in the inner ear, we have generated conditional knock-out mice (Elp3 cKO). We submitted these mice to a battery of vestibular testing (i.e. stereotyped circling ambulation, head bobbing, retropulsion, and absence of reaching response in the tail-hanging test) and found significant abnormalities. Besides, compared to wild-type mice, the auditory brain stem response of Elp3 cKO indicated that these mice are severely deaf. At the cellular level, we did not found any structural abnormalities nor cell patterning impairments that could explain deafness or balance dysfunction in Elp3 cKO mice. However, we detected some defaults in the planar orientation of their auditory hair cell bundle. In addition, the length of the kinocilium was significantly reduced both in vestibular and cochlear hair cells from Elp3 cKO mice compared with wild type littermates. We were also able to demonstrate an increased level of apoptosis in the Elp3 cKO spiral ganglion at E14.5 leading to a reduced number of fibers innervating the cochlear hair cells as well as a reduced number of their synaptic ribbons at P0 and P15. In conclusion, our results clearly showed a role of Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins acetylated by Elp3. [less ▲]

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See detailWhole organ culture in rotating bioreactor: the rat embryonic inner ear
Renauld, Justine ULg; Johnen, Nicolas ULg; Hubert, Pascale ULg et al

Poster (2013, January 28)

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is ... [more ▼]

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is composed by two types of cells: sensory hair cells and non-sensory supporting cells. All these cells are distributed according to a specific arrangement along the whole length of the cochlea. So far, the mammalian inner ear is very sensitive to damage, with no hair cell replacement or cell proliferation occurring in the cochlea. That is why understanding the mechanisms that regulate the mammalian cochlear development is important for pursuing strategies to induce sensory hair cells regeneration. Here, we present a technique of whole embryonic inner ear culture in rotating bioreactors. Besides, we compare two different culture media, DMEM and Neurobasal-A. Rat inner ears are sampled at the 16th embryonic day (E16) and grown in rotating bioreactors during 48h or six days. After 48h, semithin sections realized in the growing cochlea show the development of the ventral epithelium and ultrathin sections confirm the differentiation of the sensory hair cells. Using immunochemistry techniques on our material after 48h or six days in vitro, we show that all the cells of the organ of Corti are differentiating, whichever the culture medium used. Our preliminary results demonstrate that organ culture of the embryonic inner ear in rotating bioreactor is possible. Such a method provides an in vitro model for the investigation of developmental, regulatory, and differentiation processes that could be helpful in the understanding of the mechanisms underlying the development of the mammalian cochlea. [less ▲]

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See detailDifferentiation of Boettcher’s Cells during Postnatal Development of Rat Cochlea
Cloes, Marie; Renson, Thomas; Johnen, Nicolas et al

Poster (2013, January 28)

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See detailInteractomic map of the Ets factors family : Identification of unexpected functions in mRNA processing
Rambout, Xavier ULg; Simonis, Nicolas; Brohée, Sylvain et al

Poster (2013, January 28)

The Ets factors are a family of 27 transcription factors characterized by their unique DNA-binding domain. We aimed at building a protein-protein interaction (PPI) map (interactome) of the human Ets ... [more ▼]

The Ets factors are a family of 27 transcription factors characterized by their unique DNA-binding domain. We aimed at building a protein-protein interaction (PPI) map (interactome) of the human Ets factors in order to better define their roles and regulations in normal and oncogenic processes. The Ets interactome was built on a high-throughput yeast-two hybrid (Y2H) approach, and a literature and database curation. We identified 431 PPIs and 276 different protein partners. Clustering of the Ets interactome divided it into 24 functional subnetworks classified on their novelty index and their size. Cluster#1 was exclusively composed of newly identified interaction partners and was highly connected to the Erg subfamily of Ets factors. Gene ontology enrichment analysis revealed that it was associated to mRNA processing. In support of this result, we observed in HeLa cells that ERG and the components of cluster#1 localized in p-bodies and stress granules, physically linked cytoplasmic sites of mRNA degradation and silencing. Hence, we hypothesized that Erg proteins might have a role in post-transcriptional gene regulation and be involved in cellular mRNAs degradation. To test this hypothesis, we performed a MS2-based tethering assay and showed that the recruitment of ERG on a mRNA reporter promoted inhibition of its expression via a two-fold decrease of its half-life. ERG controls degradation of target mRNAs via different mechanisms including polysome stability, mRNA deadenylation, and p-bodies aggregation. A microarray-based appraoch identified 321 endogeneous genes whose mRNA decay rate was lowered in ERG silenced cells. Results point out the Nter domain of ERG as the predominant domain required for mRNA degradation. Importantly, oncogenic TET-Erg fusions described in AML and Ewing’s sarcoma exhibited diminished ability to degrade target mRNAs, concomitantly with the loss of the ERG Nter domain. This reinforces the important role of Erg proteins in mRNA degradation in cancer. [less ▲]

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See detailChemical probes and signaling pathways for the orphan GPCR GPR27
Dupuis, Nadine ULg; Gilissen, Julie ULg; Pirotte, Bernard ULg et al

Poster (2013, January 28)

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See detailCis-acting inhibition of MHC class I-restricted epitope presentation by Alcelaphine herpesvirus 1 genome maintenance protein
Sorel, Océane ULg; Myster, Françoise ULg; Vanderplasschen, Alain ULg et al

Poster (2013, January 18)

γ-Herpesviruses persist as latent episomes in actively dividing lymphocytes. Their consequent need to express a viral genome maintenance protein (GMP) during latency presents a potential immune target ... [more ▼]

γ-Herpesviruses persist as latent episomes in actively dividing lymphocytes. Their consequent need to express a viral genome maintenance protein (GMP) during latency presents a potential immune target. However, the GMPs from several γ-herpesviruses have evolved related strategies to limit their own MHC class I epitope presentation to cytotoxic T lymphocytes (CTLs). Alcelaphine herpesvirus 1 (AlHV-1) is a γ-herpesvirus that persists asymptomatically in its natural host, the wildebeest. However, AlHV-1 transmission to a large number of susceptible ruminants, including cattle, results in the development of a lethal lymphoproliferative disease named malignant catarrhal fever (MCF). We recently observed that the AlHV-1 GMP-homologue encoded by ORF73 is highly expressed during MCF and that the impairment of its expression renders AlHV-1 unable to induce MCF. With its 1300 aa, AlHV-1 ORF73 is the largest γ-herpesvirus GMP described to date and contains a large acidic internal repeat region that could be involved in the cis-acting CTL evasion mechanism. Here, we sought to determine the CTL evasion properties of AlHV-1 ORF73. We first performed bioinformatic analyses to characterize the protein domains. Then, we used an in vitro assay to demonstrate that ORF73 severely limits the presentation at the cell surface of an MHC class I-restricted epitope linked to ORF73 in cis. These results suggest that AlHV-1 has developed mechanisms to evade cytotoxic anti-viral response during latency. The exact mechanisms explaining the presentation defect remain to be deciphered as well as the role of the cis-acting CTL evasion mechanism of ORF73 in the pathogenesis of MCF. [less ▲]

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See detailNutrient and phytoplankton responses to external forcing in a Mediterranean coastal area unbiaised by terrestrial inputs and local activities (Calvi, Corsica)
Goffart, Anne ULg; Hecq, Jean-Henri ULg; Collignon, Amandine ULg et al

Poster (2013, January)

We present a synthesis of a long-term (1979-2011) high-resolution study performed in the oligotrophic Bay of Calvi (Corsica, northwestern Mediterranean). We explore and discuss the mechanisms controlling ... [more ▼]

We present a synthesis of a long-term (1979-2011) high-resolution study performed in the oligotrophic Bay of Calvi (Corsica, northwestern Mediterranean). We explore and discuss the mechanisms controlling the interannual variability of both nutrient and phytoplankton bloom dynamics. [less ▲]

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See detailI am (un)happy but I don't know why: Subliminal positive-self statements effects
Bustin, Gaëlle ULg; Weinberger, Joel

Poster (2013, January)

This study provides evidence that positive self-statements can increase mood among unhappy people if they are presented subliminally. In study 1, participants with low and high levels of dispositional ... [more ▼]

This study provides evidence that positive self-statements can increase mood among unhappy people if they are presented subliminally. In study 1, participants with low and high levels of dispositional happiness were presented subliminally with the two words I AM which were immediately paired with a positive word. Results revealed that being exposed to subliminal positive self-statements seems to provide a boost in mood for people with less happy dispositions. Surprisingly, opposite effects were found for participants who had joyful dispositions: exposure to subliminal positive self-statements tended to lower their mood. Study 2 confirmed these results with an implicit measure of mood. Such results suggest that subliminal messages can affect emotions and highlight the necessity of taking personality into account in unconscious cognition research. [less ▲]

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See detailValidation of a French Version of a New Anxiety Trait Scale for Children
Geurten, Marie ULg; Meulemans, Thierry ULg; Fresson, Megan ULg et al

Poster (2013)

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to ... [more ▼]

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to validate through Confirmatory Factor Analyses (CFA) the a priori three-factor structure of the French version of the anxiety trait scale for children which includes psychological (“tend to be worried about everything”), behavioral (“tend to be upset, nervous or grumbling”), and somatic symptoms (“headache complaints”) of anxiety. This scale was previously found to discriminate, with high sensitivity and specificity, children with anxiety from control group. A first CFA performed on 288 6-12 year-old children showed an acceptable fit (2/df =2.66; RMSEA=.07 and CFI=.94). A second three-factor model was constructed and showed a better fit with a new sample of 287 children (2/df =2.18; RMSEA=.06 and CFI=.96), with a lower ECVI value for the model 2. For this model, the Cronbach’s alpha for each of the subscales ranged from .71 to .86, which confirmed the good internal reliability of the scale. This study provides a new three-factor structure for this anxiety scale and proposes normative data for French-speaking children. [less ▲]

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See detailBrucella surveillance in stranded marine mammals from the North Sea
Alonso-Velasco, E.; Jauniaux, Thierry ULg; Michel, P. et al

Poster (2013)

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See detailL’agriculture périurbaine de la ville de Kinshasa et les revenus des maraîchers
Masiala Bode, Mabu; Lebailly, Philippe ULg

Poster (2013)

Ce poster vise à étudier la dynamique spatiale du site de Nzeza Nlandu, à déterminer les revenus tirés du maraîchage, à analyser le lien qui existe entre les revenus maraîchers et les superficies ... [more ▼]

Ce poster vise à étudier la dynamique spatiale du site de Nzeza Nlandu, à déterminer les revenus tirés du maraîchage, à analyser le lien qui existe entre les revenus maraîchers et les superficies emblavées et, à faire ressortir la contribution des revenus maraîchers dans les dépenses courantes des ménages exploitants. [less ▲]

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See detailEffects of diazoxide, benzothiadiazine and benzopyrane derivatives on mitochondrial proton and electron leaks of cardiomyocytes (H9C2 cell line).
Mouithys-Mickalad, Ange ULg; Ceusters, Justine ULg; Charef, M et al

Poster (2013)

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of ... [more ▼]

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of adenosine triphosphate (ATP) and reactive oxygen species (ROS). In many cancer cells those organelles become dysfunctional leading to a shift of energy metabolism from oxidative phosphorylation to active glycolysis and an increase of ROS generation. According to Warberg’ theory, cancer damage might occur at the mitochondrial level, affecting tiny structures within each cell implicated in the energy production through ATP. New insight is that mitochondria might be a good therapeutic target for metabolic syndromes, ischemia/reperfusion injury and organs transplantation. Therefore, search for novel molecules able to keep mitochondria functional are of relevant interest. Methodology: Cardiomyocytes (H9C2 cells) were from ATCC (USA) and grown till confluence. The basal cellular respiratory rate, proton and electron leaks as well as ATP production were measured with the High Resolution Oxygraphy (Oroboros, Austria). All compounds: diazoxide (DIAZ), diazoxide –related analogs (1: BPDZ-259, 2: BPDZ-444), and benzopyran derivatives (3: BPDZ-490, 4: BPDZ-711) were tested at final concentration of 10-5 M, except when specified and compared to control samples (cells with or without DMSO). Results and conclusion: The basal respiratory rate of H9C2 cells (5x106/mL) was changed depending on the chemical structure of the tested compounds: e.g. compound 3 strongly enhanced the routine respiration, while 4 displayed a marked lowering effect. In contrast, the addition of similar concentration of benzothiadiazin derivatives (1, 2) had no effect on routine respiration but also on the other respiratory parameters such as oligomycin-induced leak and ATP production. Similar profile was obtained with the reference molecule: diazoxide. Overall, our findings indicate that both diazoxide-like analogues (1 and 2) and diazoxide were without significant effect on basal respiration, ATP production, even on maximal respiration. Interestingly, two derivatives show opposite effects: compound 3 behaves as a uncoupling agent and the other one (4) exhibits a real lowering effect on respiration but that was reversible. The latter effect might be of interest if this kind of molecules could be used for further use as an agent for organ conservation during transplantation. Our results also demonstrate that diazoxide, a well-known Mito-KATP opener, did not exert its effect beside of clinical situation like ischemia/reperfusion injury. [less ▲]

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See detailThermal and structural behaviour of four industrial laurie fats
Anihouvi, Prudent; Blecker, Christophe ULg; Dombrée, Anne et al

Poster (2013)

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See detailCurrent net ecosystem exchange of CO2 in a young mixed forest: any heritage from the previous ecosystem?
Violette, Aurélie ULg; Heinesch, Bernard ULg; Erpicum, Michel ULg et al

Poster (2013)

For 15 years, networks of flux towers have been developed to determine accurate carbon balance with the eddy-covariance method and determine if forests are sink or source of carbon. However, for ... [more ▼]

For 15 years, networks of flux towers have been developed to determine accurate carbon balance with the eddy-covariance method and determine if forests are sink or source of carbon. However, for prediction of the evolution of carbon cycle and climate, major uncertainties remain on the ecosystem respiration (Reco, which includes the respiration of above ground part of trees, roots respiration and mineralization of the soil organic matter), the gross primary productivity (GPP) and their difference, the net ecosystem exchange (NEE) of forests. These uncertainties are consequences of spatial and inter-annual variability, driven by previous and current climatic conditions, as well as by the particular history of the site (management, diseases, etc.). In this study we focus on the carbon cycle in two mixed forests in the Belgian Ardennes. The first site, Vielsalm, is a mature stand mostly composed of beeches (Fagus sylvatica) and douglas fir (Pseudotsuga menziesii) from 80 to 100 years old. The second site, La Robinette, was covered before 1995 with spruces. After an important windfall and a clear cutting, the site was replanted, between 1995 and 2000, with spruces (Piceas abies) and deciduous species (mostly Betula pendula, Aulnus glutinosa and Salix aurita). The challenge here is to highlight how initial conditions can influence the current behavior of the carbon cycle in a growing stand compared to a mature one, where initial conditions are supposed to be forgotten. A modeling approach suits particularly well for sensitivity tests and estimation of the temporal lag between an event and the ecosystem response. We use the forest ecosystem model ASPECTS (Rasse et al., Ecological Modelling 141, 35-52, 2001). This model predicts long-term forest growth by calculating, over time, hourly NEE. It was developed and already validated on the Vielsalm forest. Modelling results are confronted to eddy-covariance data on both sites from 2006 to 2011. The main difference between both sites seems to rely on soil respiration, which is probably partly a heritage of the previous ecosystem at the young forest site. [less ▲]

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See detailModelling of localised gas pathways in long-term gas injection test
Gerard, Pierre; Collin, Frédéric ULg; Harrington, Jon et al

Poster (2013)

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See detailTypologie des exploitations agricoles familiales du territoire de Kipushi/RDC
Tshomba Kalumbu, John; Nkulu Mwine Fyama, Jules; Berti, Fabio ULg et al

Poster (2013)

Ce poster vise à établir la typologie structurelle et fonctionnelle des exploitations agricoles familiales du territoire de Kipushi et à identifier et analyser les facteurs de production susceptibles ... [more ▼]

Ce poster vise à établir la typologie structurelle et fonctionnelle des exploitations agricoles familiales du territoire de Kipushi et à identifier et analyser les facteurs de production susceptibles d’influencer le niveau de compétitivité de cette agriculture périurbaine. [less ▲]

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See detailExpansion of CD16+ CD56+ NK cells in vericyte® NK cell growth medium
Brohée, Laura ULg; Bastin, Renaud ULg; Wingert, S et al

Poster (2013)

Natural Killer (NK) cells play a key role in host resistance to virus and tumour. These cells are potent killers of virus infected and tumour cells via a direct recognition of the target by activation ... [more ▼]

Natural Killer (NK) cells play a key role in host resistance to virus and tumour. These cells are potent killers of virus infected and tumour cells via a direct recognition of the target by activation receptor such as NKG2D or by inducing Fcγ receptor (FcγRIII, CD16) mediated antibody dependent cellular cytotoxicity (ADCC). Current NK cell-based cancer immunotherapy aims to produce large amounts of functional NK cells, unfortunately most culture media used for NK cell expansion induced the downregulation of CD16 on NK cells. Here, we tested the impact of a new NK cell growth medium (Vericyte® from Medicyte) on CD16 expression. Sorted NK cells and peripheral blood mononuclear cells (PBMC) were cultivated in vericyte® NK cell growth medium and cells issued from these cultures were characterized in term of expansion and phenotype at several time points. After 5 days of culture, an expansion of both NK cells and PBMC was observed and maintained at least until day 20 of culture. In PBMC cultures, we observe only a small preferential NK cell growth since NK cells were around 5-10% at beginning of the culture and this percentage increased to 15% at the end of the culture. However, these cells showed a high proliferative potential when we started the culture with sorted NK cells (the proportion of contaminant cells remain low, under 5%). NK cells expressed CD56 and NKp46 and interestingly after a decreased expression of CD16 on the cell surface at day 3, this receptor was up regulated and most of the cells are CD56bright CD16bright from day 7 to day 12. According FACS FCS/SSC dot plot, NK cells acquired morphology of large activated lymphocytes and some of them expressed activation markers such CD25. Finally, these cells were able to kill efficiently tumour cell line K562. Thus our data show that vericyte® NK cell growth medium allows the expansion of functional CD16+CD56+ NK cells. Cytokine production and ADCC function are under investigation. [less ▲]

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See detailAvantages et inconvénients d’une forêt communautaire
Meunier, Quentin; Boldrini, Sylvie; Boukouendji, B. et al

Poster (2013)

Le poster présente de façon didactique les avantages et inconvénients pour une communauté villageoise de démarrer un processus de forêt communautaire au Gabon

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See detailThe neural correlates of recollection and familiarity during aging
Angel, Lucie; Bastin, Christine ULg; Genon, Sarah ULg et al

Poster (2013)

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See detailCharacterization of olefins from enzymatically interesterified palm oil
Gibon, Véronique; Maes, Jeroen; Dijckmans, Peggy et al

Poster (2013)

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See detailEnzymatic remediation of crude palm oil
Gibon, Véronique; Kodali, Sitharam; Maes, Jeroen et al

Poster (2013)

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See detailMaternal transition of prenatal undernutrition in the laying hen
Willems, Els; Wang, Yufeng; Koppenol, Astrid et al

Poster (2013)

Peer Reviewed
See detailA multiscale method for a robust detection of the default mode network.
Baquero, Katherine; Gómez, Francisco; Cifuentes, Christian et al

Poster (2013)

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See detailThe adventures of a little star
Nazé, Yaël ULg; Thonnard, Florine

Poster (2013)

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See detailExploring the sky
Nazé, Yaël ULg

Poster (2013)

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See detailThe Belgian sky
Nazé, Yaël ULg; Fontaine, Sébastien ULg

Poster (2013)

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See detailDifferentiation of Boettcher's cells during postnatal development of rat cochlea
Cloes, Marie ULg; Renson, Thomas; Johnen, Nicolas ULg et al

Poster (2013)

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See detailEffect of humic substances on in vitro rooting of alder (Alnus glutinosa (L.) Gaertn) and birch (Betula pendula Roth)
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Druart, Philippe et al

Poster (2013)

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic functionalities in any ecosystems involving soils, sediments, water and landfills. They are heterogeneous and complex carbon macromolecules. Our study aims to compare the effect of HS from différent sources on in vitro rooting of woody plants. [less ▲]

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See detailConstaining the wind collision region geometry in the WN+O binary V444 Cyg
Lomax, Jamie; Nazé, Yaël ULg; Hoffman, jennifer

Poster (2013)

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See detailCollection Efficiency and Design Requirements for Metallic Nanowire Networks in Solar Cells
Langley, Daniel ULg; Giusti, Gael; Nguyen, Ngoc Duy ULg et al

Poster (2013)

In using TCMs based on metallic nanowires it is important to determine the effect of nanowire geometry and spatial arrangement on the resulting network. To this end we have extensively simulated the ... [more ▼]

In using TCMs based on metallic nanowires it is important to determine the effect of nanowire geometry and spatial arrangement on the resulting network. To this end we have extensively simulated the effect of wire length and device size on the percolation properties of the network produced. We have performed Monte Carlo simulations of 2D conductive stick networks including for the first time stick lengths approximating nanowires which are produced experimentally. Each simulation is performed based on an average stick length but the actual lengths of the nanowires in the simulation are randomly generated with a normal distribution around the defined average length. The effects of density and length distribution on the percolation threshold are also explored. The results of such simulations are also employed to determine an elementary representative volume, which can be directly applied to a device design by allowing the determination of the nanowire density required to produce a conductive network associated with a characteristic length, such as diffusion length or pixel size. We also extend this work to the specific application of metallic nanowire networks as front electrodes in dye sensitized solar cells (DSSC), allowing a calculation of the collection efficiency as a function of network density. These calculations were based on the diffusion length of electrons generated within a DSSC and a spatial mapping of the collection efficiency function on the solar cell surface. [less ▲]

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See detailEvaluation of a new [18F] labeled tracer targeting synaptic vesicle protein 2C by ex vivo autoradiography and in vivo PET study in rat brain.
Warnock, Geoffrey; Aerts, Joël ULg; Mestdagh, Nathalie et al

Poster (2013)

Introduction The synaptic vesicle protein 2 (SV2) family is a group of integral membrane glycoproteins homologous to the major facilitator superfamily and could be involved in several neuronal diseasesa ... [more ▼]

Introduction The synaptic vesicle protein 2 (SV2) family is a group of integral membrane glycoproteins homologous to the major facilitator superfamily and could be involved in several neuronal diseasesa. The binding of the novel, no-carrier-added, [18F] labeled compound [18F]UCB-F to the SV2C isoform was evaluated in rat brain. Methods Radiochemistry No-carrier added [18F]UCB-F was obtained following the method shown in Fig. 1. The identity and purity of the tracer were evaluated by radioUPLC and chiral radioHPLC. Autoradiography Sprague Dawley rat brain sections were incubated at RT with buffered [18F]UCB-F solutions and exposed on film. Matching sections were stained with cresyl violet for structural identification. PET studies PET studies (Siemens Concorde Focus 120 µPET) were performed under isoflurane anesthesia. The tracer was injected as a bolus via the tail vein. After a 10-min transmission scan to correct for attenuation, dynamic emission data was recorded for a total of 60 min. The impact of P-glycoprotein (P-gp) activity on tracer uptake in the brain was evaluated using cyclosporine (50 mg/kg SC). Metabolite analysis During PET studies, arterial blood samples were taken for the measurement of tracer metabolites. Plasma was separated by centrifugation and proteins were acid-precipitated. Metabolites were detected using HPLC and confirmed by gamma counting. Results The tracer was obtained with a decay corrected yield of ±10%. Specific activity ranged from 10 GBq/µmol to 40 GBq/µmol. Ex vivo autoradiography showed that the binding of [18F]UCB-F to SV2C closely matched the expected distribution b (Fig.2). In vivo PET studies revealed that [18F]UCB-F briefly entered the brain, but exhibited extremely rapid washout. A large accumulation in the liver and intestines was observed. Metabolite analysis in the plasma revealed high protein binding and rapid metabolism. Inhibition of P-gp transport with cyclosporin had no clear effect on the rapid washout from the brain. Conclusions Despite a close match between [18F]UCB-F SV2C binding and the expected brain distribution, the pharmacokinetics in rat brain appear unfavorable for the use of this tracer to quantify SV2C in vivo. Acknowledgement / References a Lynch & al (2004) Proc. Natl. Acad. Sci. USA 101:9861 b Janz & Sudhof (1999) Neuroscience 94:1279 c The authors thank the Walloon Region and the FRNS Belgium for financial support. [less ▲]

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See detailChronic exercise differentially impacts perceptual or motor inhibition as a function of age: a cross-sectional study
Albinet, Cédric; Boucard, Geoffrey; Collette, Fabienne ULg et al

Poster (2013)

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See detailSelf-coverage of Leidenfrost droplets
Maquet, Laurent ULg; colinet, pierre; Moreau, Florian ULg et al

Poster (2013)

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See detailMicroRNA targeting of CoREST controls polarization of migrating cortical neurons.
Volvert, M. L.; Prevot, Pierre-Paul ULg; Pirotte, S. et al

Poster (2013)

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See detailErdheim-Chester Disease : A Monocentric Series Of 96 Patients
Haroche, Julien; Arnaud, Laurent; Cohen-Aubart, Fleur et al

Poster (2013)

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See detailDevelopmental changes of thyroid hormones in sheepshead minnow, Cyprinodon variegatus
Schnitzler, Joseph ULg; Mariavelle, Emeline ULg; Silvestre, Frédéric et al

Poster (2013)

The sheepshead minnow is widely used in ecotoxicological studies and such investigations have begun to focus on potential disruption of the thyroid axis. However, normal levels of thyroxin (T4) and 3,5,3’ ... [more ▼]

The sheepshead minnow is widely used in ecotoxicological studies and such investigations have begun to focus on potential disruption of the thyroid axis. However, normal levels of thyroxin (T4) and 3,5,3’-triiodothyronine (T3) and their developmental patterns are unknown. This study set out to determine the profiles of whole-body thyroxin (T4) and 3,5,3'-triiodothyronine (T3) levels during the development of sheepshead minnow from embryo to juvenile and adults. Couples of three females and two males were placed in breeding chambers designed for this experiment. More than 1000 eggs were collected and maintained in seawater. Embryos were selected under a dissection microscope and placed in incubation dishes (50 per dish) at 25°C. On day 8, embryos hatched and larvae were transferred to 1L beakers. For one week after hatching, larvae were fed on artemias and from 8 to 30 days post-hatch they were fed on flaked fish food. Embryos were sampled on day 0, 2, 4, 6 post-fertilization and larvae and juveniles were sampled every three days from day 0 to 28 days post-hatch. The pooled samples were taken from several incubation dishes and divided in three replicate batches of 30 individuals. Enzyme-linked immunoassay were used and validated for analysis of T4 and T3 after extraction from whole fish. At each sampling point 5 individuals were placed in formalin fixative for histology. Length and body mass were measured. Hatching success, gross in vivo observations, thyroid hormone levels and histology data will be determined and discussed in the framework to characterize the profiles of thyroid hormone levels during the development of sheepshead minnow from embryo to adult. This study establishes a baseline for thyroid hormones in sheepshead minnows, which will be vital for the understanding of thyroid hormone functions and in future studies of thyroid toxicants in this species. [less ▲]

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See detailApprovisionnement alimentaire de la ville de Bukavu : flux en provenance de l'intérieur du Sud-Kivu, du Rwanda et du Nord-Kivu
Vwima Ngezirabona, Stany ULg; Lebailly, Philippe ULg

Poster (2013)

Ce travail caractérise les flux principaux d’approvisionnements alimentaires de la ville de Bukavu en provenance de l’intérieur de la province du Sud-Kivu, du Nord-Kivu et du Rwanda issus du pointage des ... [more ▼]

Ce travail caractérise les flux principaux d’approvisionnements alimentaires de la ville de Bukavu en provenance de l’intérieur de la province du Sud-Kivu, du Nord-Kivu et du Rwanda issus du pointage des flux des produits alimentaires. [less ▲]

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See detailAltered mitochondrial oxidative phosphorylation capacity in horses suffering from polysaccharide storage myopathy
Tosi, Irène ULg; Art, Tatiana ULg; Cassart, Dominique ULg et al

Poster (2013)

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is ... [more ▼]

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is characterized by an accumulation of abnormal glycogen in myofibers due to a genetic defect in the skeletal muscle glycogen synthase (GYS1) enzyme. We hypothesized that the energetic production through the oxidative phosphorylation (OXPHOS) in muscular mitochondria might be impaired in type-1 PSSM-affected horses. Material and Methods: Eight horses with a history of exertional rhabdomyolysis were tested for the GYS1 mutation. Muscle biopsies were collected and used for histological analysis and high resolution respirometry (HRR). HRR values from 3 groups of horses (PSSM-positive horses, horses with a history of myopathy but negative to PSSM and healthy controls) were compared using a linear mixed model to take into account repeated (2-3 times) measurements made for each horse. Results: In four horses histology revealed an accumulation of abnormal glycogen in myofibers. A severe depression of the maximal OXPHOS capacity was observed by HRR in all horses with exertional rhabodmyolysis, with lower values in PSSM-positive cases. Conclusions: Our study shows a severe decreased OXPHOS capacity in PSSM-affected horses. PSSM is considered primarily a defect in glycogen synthesis but altered OXPHOS might play a central role in its pathogenesis. [less ▲]

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See detailPreserved automatic inhibition effect after 1 Hz repetitive transcranial magnetic stimulation over the supplementary motor area
D'Ostilio, Kevin ULg; CREMERS, Julien ULg; DELVAUX, Valérie ULg et al

Poster (2013)

Background: It is widely accepted that medial frontal regions are involved in voluntary action control. Indeed, Sumner et al. (2007) have recently suggested that one of the mechanisms through which the ... [more ▼]

Background: It is widely accepted that medial frontal regions are involved in voluntary action control. Indeed, Sumner et al. (2007) have recently suggested that one of the mechanisms through which the supplementary motor area (SMA) contributes to voluntary control is automatic and unconscious motor inhibition. In this study, they administered a visuo-motor subliminal masked prime task (Eimer & Schlaghecken, 2003) to two patients with micro-lesions of the SMA and demonstrated an absence of automatic and unconscious inhibition as evoked by masked prime stimuli. This finding has been supported by neuroimaging data (D'Ostilio et al., 2012). Here, the aim of our research was to corroborate this result by means of a “virtual lesion” approach. Methods: For this purpose, we examined the effects of 1 Hz rTMS (train of 20 min; stimulus intensity 120 % of resting motor threshold) over the SMA of ten healthy volunteers, previously localized by functional magnetic resonance imaging (fMRI), on reaction time (RT) performance in the subliminal masked prime task. The functional localizer experiment consisted of four blocks of sequential finger tapping and 15 s of rest after each block. Imaging data were analyzed with SPM 8 and then were imported into the Brainsight software version 2.1.5. With such system, we were able to navigate across the subjects’ brain. The peak voxel in the SMA for each subject (at a statistical threshold of p < 0.05 uncorrected) was used as a target point for the rTMS session. Results: The mean motor threshold was 50.9 % of maximal stimulator output (SD: ± 4.86 %). Wilcoxon tests showed a significant effect of compatibility on RTs (sham: Z = 2.7, p = 0.007; rTMS: Z = 2.8, p = 0.005) and accuracy rate (sham: Z = 2.5, p = 0.01; rTMS: Z = 2.1, p = 0.03), subjects being slower and making more errors in compatible trials (sham: 391.64 ± 52 ms, 87.3 % of accuracy; rTMS: 396.66 ± 37 ms, 86.3 % of accuracy) in comparison to incompatible trials (sham: 357.45 ± 36 ms, 92.5 % of accuracy; rTMS: 356.25 ± 28 ms, 92.7 % of accuracy), suggesting motor inhibition. However, this NCE was preserved after rTMS over the SMA (RTs: Z = 0.87, p = 0.39; accuracy rate: Z = 0.71, p = 0.47). Conclusions: We conclude that long trains of low intensity 1 Hz rTMS did not affect the modulation of RT by subliminal stimuli, suggesting that the SMA might not be mandatory for the implementation of this automatic process. The limitation of this study is relative to the neural efficacy argument because we are not sure that TMS was strong enough to disturb the redundant organizational processing in the SMA or that other regions were not able to compensate for the virtually lesioned area. [less ▲]

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See detailPhotoferrotrophy and Fe-cycling in a freshwater column
Llirós, M; Crowe, SA; García-Armisen, T et al

Poster (2013)

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See detailExpression of a protease of biotechnological interest cloned from C. d. collilineatus venom gland
Boldrini-Franca, Johaha; Rodrigues, RS; Santos-Silva, LK et al

Poster (2013)

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See detailAn intervention study on physical activity and cognitive functioning in people with Parkinson’s disease
Rouillard, Maud ULg; Audiffren, Michel; Albinet, Cédric et al

Poster (2013)

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See detailEtude de modes de production de charbon de bois sur l'axe Lubumbashi-Kasenga
Nge Okwe, A.; Ngoy Shutcha, M.; Nkulu Mwine Fyama, J. et al

Poster (2013)

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See detailCharacterization of structures with a complex disposition of YBCO coated conductors for magnetic shielding applications
Wera, Laurent ULg; Fagnard, Jean-François ULg; Levin, G. A. et al

Poster (2013)

An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free ... [more ▼]

An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free superconducting loop where persistent currents can flow and provide a strong attenuation of a magnetic field. Our previous works have shown that this assembly is able to shield an axial quasi static (“DC”) magnetic field and that the shielding performances depend on the aspect ratio and the number of layers. The purpose of the present work is to study experimentally the shielding efficiency of several structures with a more complex orientation and position of YBCO coated conductors. Our aim is to design a magnetic shield that would be able to shield a magnetic field directed at any angle with respect to the superconducting loops. Such a structure can be obtained by placing pairs of coated conductors sections along three orthogonal axes. All experiments are carried out at 77K. The structure is subjected to a quasi-static (“DC”) magnetic field. A Hall probe measures the three components of the local magnetic induction inside the assembly as a function of the applied magnetic induction. The shielding efficiency of the structure is characterized as a function of (i) the magnetic field amplitude, (ii) the position of the Hall probe along the three axes, and (iii) the angles between the applied magnetic field and each axis. The experimental results allow us to determine the shielding efficiency in the central part of the new 3-axes structure. Although the shielding efficiency is lowered with respect to that of the traditional 1-axis-coil geometry, measurements at different field orientations allow us to identify the role played by each of the pairs of coils in screening the external magnetic field. [less ▲]

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See detailPredictive Value of Squamo-Columnar Junction Markers and p16ink4 in Multi-Observer Classification of Cervical Precursor Lesions
Jimenez, C; Howitt, BE; Nucci, MR et al

Poster (2013)

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See detailMulti-Scale Modelisation of the Optical Second Harmonic Generation of Tyrosine-Containing Iturinic Antimicrobial Lipopeptides
Loison, Claire; Nasir, Mehmet Nail ULg; Benichou, Emmanuel et al

Poster (2013)

The optical Second Harmonic Generation (SHG) of Mycosubtilin Langmuir Layers at the air/water interface are modeled. In this tyrosine-containing lipopepeptide, the environment of the tyrosine residue is ... [more ▼]

The optical Second Harmonic Generation (SHG) of Mycosubtilin Langmuir Layers at the air/water interface are modeled. In this tyrosine-containing lipopepeptide, the environment of the tyrosine residue is critical in defining the SHG response. To propose reasonable the structure of Mycosubtilin aggregates at the air-water interface, molecular dynamics simulations (all-atom and coarse-grained) are performed. Molecular hyperpolarizability of the lipopeptide are obtained by Quantum Chemistry calculations. Finally, the SHG susceptibilities of the interface are calculated using a simple additive model. The molecular dynamics simulations suggest that lipopeptides aggregate at the interface into half-micelles, and that this phenomena is the origin of a constraint on Tyrosine orientation. In particular the C-OH bond of the Tyrosine residue has a preferential orientation along the interface normal, pointing towards the air. This inhomogneneous orientation distribution, associated with a dominant hyperpolarizability component along the C-OH bond of the Tyrosine, yields a characteristic SHG response. Comparison with experimental data gathered in our lab are proposed. [less ▲]

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See detailThe first Upper Paleolithic human remains from Belgium: Aurignacian, Gravettian and Magdalenian fossils at the “troisième caverne” of Goyet
Rougier, H.; Crevecoeur, I.; Beauval, C. et al

Poster (2013)

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See detailInduction of photosynthetic electron transfer upon anoxia in Chlamydomonas: role of hydrogenase activity and PSI-cyclic electron flow
Godaux, Damien ULg; Berne, Nicolas ULg; Remacle, Claire ULg et al

Poster (2013)

In Chlamydomonas reinhardtii, anoxic environment leads to the expression of various fermentative/anaerobic pathways. Among them, oxygen-sensitive hydrogenases catalyze the reduction of protons from ... [more ▼]

In Chlamydomonas reinhardtii, anoxic environment leads to the expression of various fermentative/anaerobic pathways. Among them, oxygen-sensitive hydrogenases catalyze the reduction of protons from reduced ferredoxin resulting in the production of molecular hydrogen. A possible role of chloroplast hydrogenase in the anaerobic induction of photosynthesis has been suggested forty years ago (Kessler, 1973) but never further explored. H2 evolution is a minor and transient phenomenon which is often considered as a safety mechanism to protect photosynthetic chain from overreduction (Melis and Happe, 2001; Hemschemeier et al., 2009). Recent data about hydrogen production in a pgrl1 (Proton Gradient Regulation like1) mutant with limited capacity for PSI-cyclic electron flow (CEF) also suggested a participation of CEF in photosynthesis reactivation after short dark-anoxic periods (Tolleter et al., 2011). Because H2 evolution is improved in pgrl1 mutant, authors came to the conclusion that H+ gradient generated by CEF strongly prevents electron supply to the hydrogenase and is thus a limitating factor for hydrogen production. The aim of our work is to further study the role of hydrogenase and CEF in the photosynthesis reactivation process after short (~1h) or long (>18h) dark-anoxic periods. We take advantage of the availability of hydrogenase-deficient mutants (hydEF, hydG) (Posewitz et al., 2005; Godaux et al., 2013) and above-mentioned CEF-deficient pgrl1 mutant. Light-induced photosynthetic electron transfer is studied by measuring hydrogen and oxygen evolution, as well as by following kinetics of chlorophyll fluorescence emission and P700 oxidoreduction. Firstly, we show that during the induction of photosynthesis after long dark-anoxic periods, there is a linear relationship between hydrogen evolution, PSI and PSII activities, meaning that an hydrogenase- dependent photosynthetic linear electron flow (LEF) mainly operates. Moreover, PSI and PSII photochemical yield are almost null in hydrogenase-deficient mutants. We conclude that hydrogenase is the main sink for photosynthetic electrons upon illumination after prolonged anoxia. Similarly, a linear correlation can be established between hydrogen evolution, hydrogenase expression/activity, and PSI or PSII photochemical yields upon adaptation to anoxia. In the next part of our work, we focus our attention on the role of PSI-CEF in the induction of photosynthesis upon anoxia. Combined measurements of PSI/PSII activities and O2/H2 evolution show that induction of photosynthesis is delayed in a Pgrl1-deficient strain. In absence of Pgrl1 protein, the H+ gradient is also lower and we thus propose that a lack of ATP is responsible for the delayed Calvin cycle reactivation, so that hydrogen production can be achieved for a longer time without inactivation of hydrogenase activity by evolved O2. These results are in good agreement with other results obtained by our group, demonstrating that state transition is a critical process for induction of photosynthesis in anoxia (Ghysels et al., accepted). In conclusion, a Pgrl1-dependent CEF seems to be in first importance to photosynthesis induction after one hour of dark-anaerobiosis adaptation, acting together with an hydrogenase dependant LEF to set favourable conditions for Calvin cycle activation. [less ▲]

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See detailDiagnosis of Pathogens responsible of pre-and post-harvest rice diseases in Madagascar
Razanakoto Mamiharisoa, Lea; De Clerck, Caroline ULg; Parisi, Olivier ULg et al

Poster (2013)

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See detailLe minerai de fer en Wallonie: une cartographie des gisements
Denayer, Julien ULg

Poster (2013)

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See detailSelf-coverage of Leidenfrost droplets
Maquet, Laurent ULg; colinet, pierre; Moreau, Florian ULg et al

Poster (2013)

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