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See detailA novel protocol for the design of artificial (β/α)8-barrel proteins
Martina, Cristina ULg; Figueroa Yévenes, Maximiliano ULg; Moretti, Rocco et al

Poster (2016)

The design of protein de novo is an emerging field in biochemistry, where artificial proteins are first designed in silico and then validated experimentally. This research, which rests mainly on our ... [more ▼]

The design of protein de novo is an emerging field in biochemistry, where artificial proteins are first designed in silico and then validated experimentally. This research, which rests mainly on our current understanding of protein structure, function, folding, stability and solubility, contributes to expand our knowledge of proteins in general. Our group has a long tradition in the design of artificial (β/α)8 -barrel proteins (called Octarellins). This fold is extremely interesting because it is widespread in nature (10% of the known proteins contain this fold) and in catalysis (it is present in 5/6 classes of enzyme). Here we present a protocol to design de novo (β/α)8-barrels with the more recent and best performing tools: Rosetta and Modeller (modelling softwares), and GROMACS (molecular dynamic simulations). First, 4000 artificial backbone structures were created with the use of modelling packages Rosetta and Modeller. 54 out of them were selected as targets for the following steps of sequence design and energy minimization (10 cycles), in order to find the best sequence to fit each target. More than 10000 different artificial sequences were created. Selection steps were performed in order to reduce the number of candidates for each target and the best ones were subjected to molecular dynamic simulation. Among this, 5 models were finally chosen for gene synthesis and experimental validation, and are currently being tested for expression in E. coli and preliminary purification. [less ▲]

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See detailTowards robust blood biomarkers for residual feed intake.
Schroyen, Martine ULg; Feye, Kristina M; Nguyen, Yet et al

Poster (2016)

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See detailThe Fractal Nature of Mars Topography Analyzed via the Wavelet Leaders Method
Kleyntssens, Thomas ULg; Deliège, Adrien ULg; Nicolay, Samuel ULg

Poster (2016)

This work studies the scaling properties of Mars topography based on Mars Orbiter Laser Altimeter (MOLA) data through the wavelet leaders method (WLM). This approach shows a scale break at 15 km. At small ... [more ▼]

This work studies the scaling properties of Mars topography based on Mars Orbiter Laser Altimeter (MOLA) data through the wavelet leaders method (WLM). This approach shows a scale break at 15 km. At small scales, these topographic profiles display a monofractal behavior while a multifractal nature is observed at large scales. The scaling exponents are greater at small scales. They also seem to be influenced by latitude and may indicate a slight anisotropy in topography. [less ▲]

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See detailHost Interleukin 6 and Indoleamine 2,3 Dioxygenase Regulate Inflammation in the Brain during Graft Versus Host Disease
Belle, Ludovic ULg; Koester, Erin; Hansen, Emily et al

Poster (2016)

Graft versus host disease (GVHD) commonly induces pathological damage in peripheral target organs such as the skin, liver and gastrointestinal tract leading to well characterized organ-specific clinical ... [more ▼]

Graft versus host disease (GVHD) commonly induces pathological damage in peripheral target organs such as the skin, liver and gastrointestinal tract leading to well characterized organ-specific clinical manifestations. A number of studies, however, have shown that patients with GVHD can also have behavioral and mood alterations that can affect overall cognitive function and lead to significant impairments in quality of life. The extent to which GVHD contributes to cognitive dysfunction and induces inflammation within the central nervous system (CNS), however, has not been critically examined. To address this question, we conducted studies using two well-defined murine GVHD models [C57BL/6(H-2b)→Balb/c (H-2d) and B10.BR(H-2k)→B6 (H-2b)]. We observed that there was a significant increase in the number of donor-derived CD4+ and CD8+ T cells in the brains of GVHD recipients early (days 7 -14) and late (day 42) post transplantation compared to BM controls. Histological studies revealed activated microglial cells and CD3+ T cell infiltration in the periventricular regions of brains in GVHD recipients that were not present in BM animals. Real time q-PCR analysis also demonstrated significant increases in IFN-γ, TNF-α, and IL-6 mRNA expression indicative of a proinflammatory state. Notably, GVHD animals exhibited behavioral changes in the forced swim test and elevated plus maze which are validated assays of stress coping and anxiety, respectively. Since IL-6, in particular, plays a pivotal role in GVHD pathogenesis in murine models and humans, we examined whether blockade of IL-6 signaling altered neuroinflammation. Animals treated with an anti-IL-6R antibody had a significant reduction in the number of donor-derived CD4+ and CD8+T cells in the brain compared to isotype control-treated mice. Anti-IL-6R treatment of GVHD mice also resulted in significant reductions in IFN-γ, TNF-α, and IL-6 mRNA and normalized behavior in the forced swim test, indicative of a decreased inflammatory response. Since IL-6 is produced by a wide variety of cells, including microglial and T cells, both donor and recipient cells have the potential to modulate GVHD severity within the CNS. To define whether donor or host IL-6 production was most critical for inducing neuroinflammation, experiments were conducted employing IL-6-/- mice as either donors or recipients. Whereas the absence of IL-6 in donor-derived cells had no impact on the degree of inflammation within the CNS, recipient animals that lacked IL-6 had a significant decrease in the number of donor-derived T cells which accumulated in the brain as well as a marked reduction in inflammatory cytokines, indicating that host IL-6 production was critical. To define the downstream pathways of IL-6-mediated CNS inflammation, we examined the role of indoleamine 2,3-dioxygenase (IDO) since IL-6 has been shown to upregulate IDO-1 expression under inflammatory conditions. We observed that IDO-1 mRNA levels were significantly increased in the brains of GVHD animals, and that blockade of IL-6 signaling resulted in a marked decrease in IDO mRNA levels. Additionally, transplantation studies using IDO-/- mice revealed that host, but not donor, IDO production was required for maximal inflammatory effects. Serotoninergic projections to the prefrontal cortex (PFC), in particular, are sensitive to inflammation and contribute to stress coping behavior. Therefore, to further interrogate this pathway, we performed quantitative mass spectrometry of brain extracts from the PFC. We found that tryptophan and 5HT concentrations were not different between BM and GVHD groups. However, there was an increase in the IDO product, kynurenic acid, in GVHD recipients consistent with an increase in brain IDO expression. To provide additional support for the premise that IL-6 effects were mediated through the IDO pathway, recipient mice were treated with either 1-methyltryptophan (1-MT), a completive inhibitor of IDO, or a vehicle control. GVHD mice treated with 1-MT had decreased accumulation of T cells in the brain and normal behavior in the forced swim test, demonstrating that inhibition of IDO abrogated CNS inflammation and behavioral changes in the presence of intact IL-6 signaling. In summary, these studies demonstrate that host IL-6 and IDO regulate inflammation and adversely impact behavioral function within the brain during GVHD through the tryptophan metabolic pathway. [less ▲]

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See detailIdentification d’indicateurs de la transition forestière en lien avec les impacts de la déforestation sur les socio-écosystèmes en Afrique centrale
Gillet, Pauline ULg; Vermeulen, Cédric ULg

Poster (2015, December 26)

La théorie de la transition forestière lie l’évolution du couvert forestier au temps (Mather, 1992). Dans la première phase, le couvert forestier est très dense. Il diminue ensuite avec l’augmentation de ... [more ▼]

La théorie de la transition forestière lie l’évolution du couvert forestier au temps (Mather, 1992). Dans la première phase, le couvert forestier est très dense. Il diminue ensuite avec l’augmentation de la densité de population qui pratique la défriche pour la production agricole et utilise le bois pour répondre aux besoins en énergie et en bois d’œuvre. Dans un troisième temps, l’économie se spécialise, l’agriculture s’intensifie et est pratiquée sur les terres les plus productives et le rôle de l’écosystème forestier dans l’économie diminue. L’abandon des terres les moins productives permet la recolonisation forestière visible sur la courbe de transition. La présente étude tente d’identifier et de proposer des indicateurs d’occupation spatiale permettant, sur base d’images de télédétection, de prévoir la position réelle d’un site sur la courbe de transition forestière. Ensuite, l’étude des socio-écosystèmes en lien avec la transition forestière permet de démontrer l’impact de la diminution du couvert forestier sur le fonctionnement des sociétés humaines. Nous nous sommes penchés sur différents aspects caractérisant les activités des populations locales dans trois sites d’étude situés sur un gradient de déforestation en Afrique centrale tels que la composition de repas et l’origine des aliments. On observe que la déforestation induit une diminution des composants issus des activités fortement liées à l’écosystème telles que la chasse, la pêche ou la collecte au profit de l’agriculture et de l’élevage. [less ▲]

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See detailOrganocatalytic coupling of carbon dioxide with epoxides: the unexpected booster effect of fluoroalcohols
Grignard, Bruno ULg; Gennen, Sandro ULg; Alves, Margot ULg et al

Poster (2015, December 19)

Valorising CO2 as a C1 feedstock for producing added value building blocks is seducing as it is a free and in exhaustive waste resulting from human activity. Carbon dioxide is a thermodynamically and ... [more ▼]

Valorising CO2 as a C1 feedstock for producing added value building blocks is seducing as it is a free and in exhaustive waste resulting from human activity. Carbon dioxide is a thermodynamically and kinetically stable molecule that can be converted into cyclic carbonates by coupling with epoxides. Cyclic carbonates are valuable products that find applications as solvents, electrolytes or as monomers for polyurethanes synthesis. Although the CO2/epoxide coupling reaction has been extensively studied, the development of organocatalysts that are highly efficient under mild experimental conditions still remains a challenge. Onium salts are the most common catalysts that show reasonable catalytic activity at high pressure (> 100 bars) and high temperature (> 100°C) only. Fortunately, the efficiency of these organocatalysts can be improved by addition of appropriate hydrogen bond donors activators (HBD). In this talk, we will report the development of a new highly efficient catalytic platform consisting in an onium halide salt combined with HBD activators for the fast and solvent-free synthesis of cyclic carbonates by coupling CO2 with epoxides. The cocatalytic effect of series of HBDs will be demonstrated by detailed online kinetics studies under pressure using Raman or IR spectroscopy. We will show that our new organocatalytic platform facilitates the fast conversion of epoxy groups into cyclic carbonates under mild experimental conditions, and can be easily implemented to the modification of epoxidized vegetable oils. The synergistic effects between HBDs and onium salt will be highlighted by a detailed mechanistic study of the reaction through DFT calculations. [less ▲]

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See detailHeat tracer and solute tests in an alluvial aquifer: field experiment and inverse modelling
Dassargues, Alain ULg; Klepikova, Maria; Jamin, Pierre ULg et al

Poster (2015, December 18)

Using heat as an active tracer in different types of aquifers is a topic of increasing interest. In this study, we investigate the potential interest of using heat tracer tests for characterization of a ... [more ▼]

Using heat as an active tracer in different types of aquifers is a topic of increasing interest. In this study, we investigate the potential interest of using heat tracer tests for characterization of a shallow alluvial aquifer. A thermal tracer test was conducted in the alluvial aquifer of the Meuse River, Belgium. The tracing experiment consisted in simultaneously injecting heated water and a dye tracer in a piezometer and monitoring the evolution of groundwater temperature and tracer concentration in the recovery well and in monitoring wells. To get insights in the 3D characteristics of the heat transport mechanisms the space-filling arrangement of observation wells was used. The breakthrough curves measured in the recovery well showed that heat transfer in the alluvial aquifer is slower and more dispersive than solute transport. Recovery is very low for heat while in the same time it is measured as relatively high for the solute tracer. This is due to the fact that heat diffusion is larger than molecular diffusion, implying that exchange between groundwater and the porous medium matrix is far more significant for heat than for solute tracers. Temperature breakthrough curves in other piezometers are contrasted with what would be expected in an ideal layered aquifer. They reveal strongly unequal lateral and vertical components of the transport mechanisms. The observed complex behavior of the heat plume was explained by the groundwater flow gradient on the site and heterogeneities of hydraulic conductivity field. By using numerical model of heat and flow coupled with pilot points inverse approach main preferential paths were characterized. [less ▲]

Detailed reference viewed: 40 (5 ULg)
See detailNon-viral vectors for gene delivery: cationic polymers
Dréan, Mathilde; Debuigne, Antoine ULg; Rieger, Jutta ULg et al

Poster (2015, December 17)

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See detailQUANTIFYING CHEMICAL REACTIONS BY USING MIXING ANALYSIS IN GROUNDWATER-RIVER INTERFACE
Jurado Elices, Anna ULg; Vázquez-Suñé, Enric; Carrera, Jesus et al

Poster (2015, December 16)

This work is motivated by a sound understanding of the chemical processes that affect the organic pollutants in an urban aquifer. Urban aquifers may suffer pollution from different recharge sources such ... [more ▼]

This work is motivated by a sound understanding of the chemical processes that affect the organic pollutants in an urban aquifer. Urban aquifers may suffer pollution from different recharge sources such as leakage from sewer and septic systems, seepage from rivers, seawater intrusion, and losses from water supply network. As a result, a wide range of organic pollutants are found in urban aquifers (Fig. 1). Since these pollutants reach groundwater environment, their occurrence depends on simultaneous transport and biogeochemical processes. However, the quantification of these processes is not an easy task. The objective of this work is to propose an approach to quantify the chemical processes that occurs when river water infiltrates an aquifer. [less ▲]

Detailed reference viewed: 29 (6 ULg)
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See detailFirst Analysis of Martian Nightglow Seasonal Variations Observed by MAVEN's Imaging Ultraviolet Spectrograph
Stiepen, Arnaud ULg; IUVS team

Poster (2015, December 15)

We report results from a study of nitric oxide nightglow over the northern hemisphere of Mars during winter and southern hemisphere during equinox. We present observations of the nitric oxide δ and γ band ... [more ▼]

We report results from a study of nitric oxide nightglow over the northern hemisphere of Mars during winter and southern hemisphere during equinox. We present observations of the nitric oxide δ and γ band emissions between 190 and 270 nm by the Imaging UltraViolet Spectrograph on the MAVEN spacecraft. The emission reveals recombination of N and O atoms dissociated on the dayside of Mars, and is known to trace upper atmosphere hemispheric circulation patterns. We extend previous studies to higher latitudes, finding brighter emissions closer to the poles as predicted from global circulation models. Statistically significant variability suggests variation of the fluxes of N and O carried from the dayside thermosphere, as well as possible variations of the wind pattern in the nightside thermosphere and mesosphere. [less ▲]

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See detailSpatial and Temporal Variations in the Partial Pressure and Emission of CO2 and CH4 in and Amazon Floodplain Lake
Forsberg, BR; Amaral, JH; Barbosa, P et al

Poster (2015, December 14)

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See detailINVESTIGATING MODEL DEFICIENCIES IN THE GLOBAL BUDGET OF ETHANE
Tzompa-Sosa, Z. A.; Keller, C. A.; Turner, A. J. et al

Poster (2015, December 14)

Detailed reference viewed: 35 (11 ULg)
See detailModeling Pacific Northwest carbon and water cycling using CARAIB Dynamic Vegetation Model
Kim, John B; Dury, Marie ULg; Still, Christopher J et al

Poster (2015, December 14)

While uncertainties remain regarding projected temperature and precipitation changes, climate warming is already affecting ecosystems in the Pacific Northwest (PNW). Decrease in ecosystem productivity as ... [more ▼]

While uncertainties remain regarding projected temperature and precipitation changes, climate warming is already affecting ecosystems in the Pacific Northwest (PNW). Decrease in ecosystem productivity as well as increase in mortality of some plant species induced by drought and disturbance have been reported. Here, we applied the process-based dynamic vegetation model CARAIB to PNW to simulate the response of water and carbon cycling to current and future climate change projections. The vegetation model has already been successfully applied to Europe to simulate plant physiological response to climate change. We calibrated CARAIB to PNW using global Plant Functional Types. For calibration, the model is driven with the gridded surface meteorological dataset UIdaho MACA METDATA with 1/24-degree (~4-km) resolution at a daily time step for the period 1979-2014. The model ability to reproduce the current spatial and temporal variations of carbon stocks and fluxes was evaluated using a variety of available datasets, including eddy covariance and satellite observations. We focused particularly on past severe drought and fire episodes. Then, we simulated future conditions using the UIdaho MACAv2-METDATA dataset, which includes downscaled CMIP5 projections from 28 GCMs for RCP4.5 and RCP8.5. We evaluated the future ecosystem carbon balance resulting from changes in drought frequency as well as in fire risk. We also simulated future productivity and drought-induced mortality of several key PNW tree species. [less ▲]

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See detailEvaluating the performance of short-term heat storage in alluvial aquifer with 4D electrical resistivity tomography and hydrological monitoring
Robert, Tanguy; Paulus, Claire; Bolly, Pierre-Yves et al

Poster (2015, December 14)

In the context of energy demand side management (DSM), energy storage solutions are needed to stock energy during high production periods and recover energy during high demand periods. Among currently ... [more ▼]

In the context of energy demand side management (DSM), energy storage solutions are needed to stock energy during high production periods and recover energy during high demand periods. Among currently studied solutions, storing energy in the subsurface through heat pumps and/or exchangers (thermal energy storage) is relatively simple with low investment costs. However, the design and functioning of such systems have strong interconnections with the geology of the site which may be complex and heterogeneous, making predictions difficult. In this context, local temperature measurements are necessary but not sufficient to model heat flow and transport in the subsurface. Electrical resistivity tomography (ERT) provides spatially distributed information on the temperature distribution in the subsurface. In this study, we monitored, with 4D ERT combined with multiple hydrological measurements in available wells, a short-term heat storage experiment in a confined alluvial aquifer. We injected heated water (ΔT=30K) during 6 hours with a rate of 3 m³/h, stored during 3 days, and then we pumped it back to estimate the energy balance. We collected ERT data sets using 9 parallel profiles of 21 electrodes and cross-lines measurements. Inversion results clearly show the ability of ERT to delimit the thermal plume growth during injection, the diffusion and decrease of temperature during storage, and the decrease in size after pumping. Quantitative interpretation of ERT is difficult at this stage due to strong spatial variations of the total dissolved solid content in the aquifer, due to historical chloride contamination of the site. Energy balance shows that up to 75% of the energy can be easily recovered with an adapted strategy in the context of DSM. Short-term heat storage in alluvial aquifer is efficient and ERT is a valuable tool to image and estimate the temperature distribution in the subsurface. [less ▲]

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See detailAnalyse de la répartition spatiale des restes fauniques du site éémien de Caours. Apport des systèmes d'information géographique.
Moreau, Gwénaëlle ULg

Poster (2015, December 12)

The Palaeolithic deposit of Caours, located in the Somme, is the only Eemian site in Northern Europe in interglacial context. This site show an incredible conservation of faunal remains spread out over a ... [more ▼]

The Palaeolithic deposit of Caours, located in the Somme, is the only Eemian site in Northern Europe in interglacial context. This site show an incredible conservation of faunal remains spread out over a large area and a recurrence Human occupation. That gave us a large amount of data about this period of Neanderthal history. We now questioning about the choice of this locality and the spatial organisation of Neanderthal. Then, spatial analysis using the Geographic Information System (GIS) appear to being a useful tool to answer it. We show the consequent availability of water, prey and tool raw material. Neanderthalians was opportunistic hunters of cervidae. There has also eat a heavier prey as aurochs and rhino by trapping or scavenging. Then Neanderthal of Caours butchering with tool made in-situ eating the meat and the marrow. [less ▲]

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See detailThe ICOS Vielsalm Terrestrial Observatory: long term greehouse gas flux measurements
Hurdebise, Quentin ULg; De Ligne, Anne ULg

Poster (2015, December 10)

Presentation of the Vielsalm ICOS Terrestrial Observatory: site management, data available, data access, flux temporal dynamic and flux relation with climate variables.

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See detailEvolution récente du trait de côte dans le Golfe de Guinée: Exemple du Togo et du Bénin (2000-2015)
Ozer, Pierre ULg; Hountondji, Yvon-Carmen; de Longueville, Florence

Poster (2015, December 10)

L’interface terre-mer est un environnement extrêmement fragile. De par le monde, les littoraux sont menacés par une multitude de facteurs parfois naturels et le plus souvent anthropiques. Ainsi, aux ... [more ▼]

L’interface terre-mer est un environnement extrêmement fragile. De par le monde, les littoraux sont menacés par une multitude de facteurs parfois naturels et le plus souvent anthropiques. Ainsi, aux perturbations occasionnées aux zones côtières par les infrastructures portuaires, les barrages, les prélèvements de sédiments à même la plage ou encore l’extension urbaine, s’ajoutent les conséquences des changements climatiques, dont l’élévation du niveau des océans. Nous proposons dans cet article d’évaluer la récente évolution du trait de côte au Togo et au Bénin où plusieurs études localisées démontrent que les plages sableuses connaissent des modifications (accumulation ou érosion) majeures ces dernières décennies. Dans le but d’avoir une vision globale de la dynamique côtière sur les 152 kilomètres de plages de ces deux pays d’Afrique de l’Ouest, nous utilisons les images à haute définition spatiale disponibles en open access sur Google Earth. Dans cette étude exploratoire, nous analysons le trait de côte par sections de 1 kilomètre entre 2002 et 2015 en estimant systématiquement l’évolution moyenne en mètre par an. Globalement, il appert que si certaines sections connaissent une accumulation, c’est uniquement dû au fait que celles-ci se trouvent en amont d’un obstacle majeur, comme la jetée d’un port. Les sections relativement stables sont uniquement liées à la présence de mesures de protection, comme des épis. Partout ailleurs, les littoraux subissent un processus érosif pouvant être impressionnant avec des reculs moyens pouvant dépasser les 10 mètres par an. [less ▲]

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See detailImpact of shoreline changes on population in Cotonou, Benin
de Longueville, Florence; Hountondji, Yvon Carmen; Gemenne, François ULg et al

Poster (2015, December 10)

The coast of Cotonou (Benin) is affected by an intensive coastal erosion for several decades, mainly due to unsuitable port infrastructures and human activities. In the future, the process will very ... [more ▼]

The coast of Cotonou (Benin) is affected by an intensive coastal erosion for several decades, mainly due to unsuitable port infrastructures and human activities. In the future, the process will very likely be worsen by sea level rise as a consequence of global warming. In this study, we use a combination of very high resolution satellite images from Google Earth recorded in 2002, 2011, 2013 and 2014 and carry out field missions in September 2012, September 2013, July 2014 and February 2015 to assess the retreat of the coastline and to understand the dynamic of population in the risk area. Multi-temporal analyses of satellite images show that nearly 93 hectares of land (including 40 hectares off) were progressively eroded by the sea between 2002 and 2014 on the stretch of the first eight kilometers at the east of the Safiato groyne. This corresponds to an average coastline’s retreat of 115 meters in 13 years, with variations from 38 to 145 meters, depending on the location. Thanks to the comparison between the images of 2002 and 2014, we assess that around 765 houses disappeared by the encroachment of the sea over the distance of 8 km to the East of the Safiato groyne, of which 60 villas and about 705 makeshift houses (informal settlements). Well-off population leave the risk area when their houses are threatened and go inland, fishermen prefer to stay in the risk area to be close to their activities and precarious population have no financial and social capitals to migrate, they are trapped in the risk area. Other precarious population coming from somewhere else settle in the coastal area and grow the at-risk population. The use of intermediate images and the results of field works confirm a rapid process of settlement/destruction of makeshift houses in the coastal area. Poor people carry out simply short displacements in the risk area. There is an urgent need to secure these populations. Update and respect of urban planning regulations, good governance, cooperation with other countries and involvement of local communities are all factors that are expected to reach this objective. [less ▲]

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See detailNitrous oxide fluxes by croplands: Towards a better understanding of emission mechanisms and dynamics
Lognoul, Margaux ULg; Broux, François ULg; SALERNO, Giovanni ULg

Poster (2015, December 10)

Nitrous oxide is a greenhouse gas that can be emitted by crops. Our poster summarizes the emission mechanisms and implied variables and presents preliminary results obtained with eddy covariance on winter ... [more ▼]

Nitrous oxide is a greenhouse gas that can be emitted by crops. Our poster summarizes the emission mechanisms and implied variables and presents preliminary results obtained with eddy covariance on winter wheat and automated dynamic closed chambers on maize. [less ▲]

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See detailDesign of new delivery systems for therapeutic proteins based on calcium carbonate microspheres
Ramalapa, Bathabile ULg; Crasson, Oscar ULg; Vandevenne, Marylène ULg et al

Poster (2015, December 10)

There exists a constant need for delivery systems that are biocompatible, offer bioactives protection from premature degradation and allow for targeted delivery and controlled release. Calcium carbonate ... [more ▼]

There exists a constant need for delivery systems that are biocompatible, offer bioactives protection from premature degradation and allow for targeted delivery and controlled release. Calcium carbonate (CaCO3) is one such system that has gained great favour for employment in the biomedical field due to possibilities of controlling size, morphology and crystalline forms of particles by tuning the synthesis conditions. CaCO3 has demonstrated ability to increase safety, stability and overall efficiency of protein therapeutics. The aim of the present work was to assess the significance of polysaccharide-protein complexes in enhancing the encapsulation of proteins in CaCO3 microspheres. A Chitin Binding Domain (ChBD), reported to have affinity for hyaluronic acid, was inserted on β-lactamase enzyme to develop a chimeric protein. The chimeric protein retained the activity of the enzyme and the binding properties and was encapsulated in CaCO3 microspheres by a super critical CO2 (ScCO2) process using hyaluronic acid as a templating agent. The particles were characterised in terms of size, zeta potential, morphology and protein loading. The results obtained confirmed the affinity of the ChBD to hyaluronic acid towards the production of stable, vaterite microparticles. Protein assays demonstrated that the ChBD enhanced the encapsulation of protein by up to 10 fold. Confocal images also suggested high encapsulation of the chimeric protein compared to native protein. Thus the production of polysaccharide-protein complexes seems effective in enhancing the encapsulation of proteins in CaCO3 microparticles using the ScCO2 process. Moreover this method will further be used to enhance encapsulation of therapeutic proteins such as growth factors for bone and cartilage regeneration. [less ▲]

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See detailAnalytical developments to assess the crashworthiness of an offshore wind turbine jacket impacted by a ship
Pire, Timothée ULg; Le Sourne, Hervé; Buldgen, Loïc ULg et al

Poster (2015, December 10)

As the number of offshore wind farms is constantly increasing, impacts between ships and wind turbines is becoming a major concern. Nowadays, design offices use finite elements simulations to assess the ... [more ▼]

As the number of offshore wind farms is constantly increasing, impacts between ships and wind turbines is becoming a major concern. Nowadays, design offices use finite elements simulations to assess the crashworthiness of offshore supporting structures, which give accurate results but is time demanding. There is therefore a need for a faster method, especially during the pre-design stage. The purpose of this research is to develop analytical formulations, based on the so-called continuous elements method in order to compute the resistance of offshore wind turbine jackets when submitted to an impact. [less ▲]

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See detailCarte des risques de la baie de Tipaza (Algérie)
Nouri, Myriem ULg; Ozer, André ULg

Poster (2015, December 10)

Le littoral algérien a toujours été un territoire propice à l’établissement humain. En l’espace de 45 années, il a subi une urbanisation intense qui a développé une vulnérabilité multiple face aux ... [more ▼]

Le littoral algérien a toujours été un territoire propice à l’établissement humain. En l’espace de 45 années, il a subi une urbanisation intense qui a développé une vulnérabilité multiple face aux phénomènes naturels extrêmes. La zone d’étude, appelée dans ce travail « Baie de Tipaza », est l’un des espaces littoraux les plus évolutifs de l’aire métropolitaine d’Alger. Cette évolution lui a valu le titre de zone à très haute vulnérabilité dans l’espace algérien en termes de catastrophes naturelles. L’étude effectuée est une étude rétrospective, multiple et de constat. Son objectif est de présenter une carte des risques côtiers majeurs dans la zone-type. Les critères de jugement se basent sur des faits enregistrés et datés avec une étude géolocalisée grâce à des outils de télédétection et de système d’information géographique (SIG). Nous avons identifié trois (03) risques majeurs exerçant leur impact sur le territoire en étude : le risque sismique, les inondations et l’érosion du littoral. L’aléa sismique reste le plus dommageable car notre zone d’étude appartient au grand système actif de failles délimitant les plaques africaine et eurasiatique. Les séismes au large peuvent aussi causer des tsunamis. En ce qui concerne les inondations, l’irrégularité des pluies et leur soudaineté engendrent un caractère torrentiel au réseau hydrologique par des inondations de type « flash flood ». Quant à l’évolution littorale, la côte de Tipaza est globalement en recul permanent avec des variations entre -1.00 et - 2.70 m/an. Ces trois phénomènes exercent leur action sur un terrain de plus en plus urbanisé à très forte densité où 1.8% de la population algérienne est établie, avec un taux d’accroissement entre 1998 et 2008 de 2.1%. Cette pression démographique très importante se matérialise à travers des extensions urbaines non contrôlées et une évolution dirigée vers le littoral. La comparaison entre les images CORONA de 1967 et WORLDVIEW de 2010 montre une densification intense et continue de cette partie du littoral. Les résultats cités en amont, nous montrent l’ampleur de la vulnérabilité de la baie. Le risque sismique est permanent, il exerce un impact direct sur les constructions et induit des dégâts matériels et sociaux immédiats d’où la classification du site en « Zone III ». Les inondations se sont souvent produites suite à des événements ordinaires et saisonniers n’ayant rien de remarquables, mais accentuées essentiellement par l’urbanisation anarchique qui favorise une perméabilisation non contrôlée des sols, par la défaillance des réseaux d’assainissement et le gonflement des oueds dû à l’accumulation de décombres et de détritus surtout à leurs embouchures. Quant à l’érosion de la bande littorale, elle est causée par l’interruption du transit sédimentaire causée à l’amont par la présence de barrages qui bloquent les alluvions et par une surexploitation locale des sédiments de plages pour l’industrie du bâtiment. En outre, une urbanisation trop proche de la côte perturbe l’équilibre fragile du littoral. Ce travail limité à la baie de Tipaza a pour but de cartographier les données disponibles, il fait partie d’une étude de vulnérabilité de la zone urbanisée ou à urbanisation future de la bande côtière algérienne. Cette carte des risques permettra d’émettre des recommandations et des plans de gestion et sera un outil d’aide à la décision. [less ▲]

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See detailLa traduction de l’humour dans la poésie orale peule du Cameroun
Oumarou Mal Mazou, Rachid ULg

Poster (2015, December 09)

Le poster présente le projet de thèse en cours de finalisation à l'Université de Liège

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See detailOn the LZ distance for dereplicating redundant prokaryotic genomes
Léonard, Raphaël ULg; Baurain, Denis ULg; Kerff, Frédéric ULg et al

Poster (2015, December 07)

The fast-growing number of available prokaryotic genomes, along with their uneven taxonomic distribution, is a prob- lem when trying to assemble broadly sampled genome sets for phylogenomics and ... [more ▼]

The fast-growing number of available prokaryotic genomes, along with their uneven taxonomic distribution, is a prob- lem when trying to assemble broadly sampled genome sets for phylogenomics and comparative genomics. Indeed, most of the new genomes belong to the same subset of hyper-sampled phyla, such as Proteobacteria and Firmicutes, or even to single species, such as Escherichia coli (almost 2000 genomes as of Sept 2015), while the continuous flow of newly discovered phyla prompts for regular updates. This situation makes it difficult to maintain sets of representative genomes combining lesser known phyla, for which only few species are available, and sound subsets of highly abundant phyla. An automated straightforward method is required but none are publicly available. The LZ distance, in conjunction with the quality of the annotations, can be used to create an automated approach for selecting a subset of represen- tative genomes without redundancy. We are planning to release this tool on a website that will be made publicly available. [less ▲]

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See detailFunctional characterization of FRD3,  a citrate transporter, in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit; Spielmann, Julien ULg et al

Poster (2015, December 03)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

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See detailHuman Adult Bone Marrow and Adipose Tissue Harbor Stem Cells with Neural Crest Characteristics.
Coste, Cécile ULg; Neirinckx, Virginie; Rogister, Bernard ULg et al

Poster (2015, December 03)

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See detailAFM study of the penicillin receptor BlaR1 of Bacillus licheniformis
Mescola, Andrea ULg; Dauvin, Marjorie ULg

Poster (2015, December 03)

In presence of β-lactamines, some strains of B. licheniformis secret a β-lactamase that hydrolyses the antibiotic, making the bacteria resistant to penicillin. Staphylococcus aureus possesses a very ... [more ▼]

In presence of β-lactamines, some strains of B. licheniformis secret a β-lactamase that hydrolyses the antibiotic, making the bacteria resistant to penicillin. Staphylococcus aureus possesses a very similar system, allowing the expression of a β-lactamase in response to the presence of antibiotic outside the cell . In both strains, cells are able to sense the antibiotic in the medium through a membrane penicillin receptor BlaR1. Up to now, the 3D structure addressing the receptor in its entire form is not available, mainly due to low production yields and degradation. Here we show production, purification and reconstitution of the entire membrane protein in a lipid bilayer and a first AFM study of intra-molecular forces involved in the activation process of BlaR1. [less ▲]

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See detailCompensatory Metabolism Promotes Cancer Cell Adaptation to HDAC5 Silencing
Hendrick, Elodie ULg; Peixoto, Paul; Polese, Catherine et al

Poster (2015, December 03)

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See detailVariability in almond oil chemical traits from traditional cultivars from eastern Morocco
Houmy, Nadia; Belhaj, K.; Abid, M. et al

Poster (2015, December 03)

In Morocco, cultivation of almond tree (Prunus amygdalus L.) constitutes the second most important plantation of fruit trees after olive growing. It is mostly cultivated in two regions, « Taza, Al ... [more ▼]

In Morocco, cultivation of almond tree (Prunus amygdalus L.) constitutes the second most important plantation of fruit trees after olive growing. It is mostly cultivated in two regions, « Taza, Al Houceima Taounate » in the north and « Souss Massa Draa » in the south. Almond genetic resources (Marcoma, Fournat, Ferragnes/Ferraduel and Beldi), cultivated in eastern Morocco were studied during two consecutive crop years in order to evaluate variations in kernel oil yield, fatty acid profiles, oleic /linoleic (O/L) ratio and almond oils oxydative stability (OSI,evaluated by rancimat tests) in comparison to monovarietal olive oils. Almond kernel total oil (AO), Oleic acid (C18:1), Linoleic acid (C18:2), O/L-ratio, and tocopherol contents range between: 48 - 62% for kernel total oil; 65- 77.5% for C18:1; 17- 25% for C18:2; 2.5-4 for O/L ratio and 370 - 675 μg/g oil for tocopherols, respectively. We conclude that the genotype is the main variability source for all these chemical traits of AOs. Results obtained from Ferragnes/Ferraduel may be of interest for almond breeding focused to improve kernel oil yield and fatty acid profile. Besides, tocopherols contents of AOs seem to be the most important contributor for their stability to oxidation, even though compared to monovarietal olive oils, stability of AOs were very low and OSI value range between 20-27 hours. This fragility of AOs is due to their high content of unsaturated fatty acid which not allows their use for cooking or storage for long period. However, almond oils could have many applications in the food industry as in cosmetic [less ▲]

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See detailNo favorable effect of reduced tillage on microbial communities in a silty loam soil (Belgium)
Degrune, Florine ULg; Theodorakopoulos, Nicolas ULg; Dufrêne, Marc ULg et al

Poster (2015, December 01)

To date, only a few studies have applied metagenomics to investigate the influence of different tillage regimes and types of crop residue management on soil microbial communities. These studies were ... [more ▼]

To date, only a few studies have applied metagenomics to investigate the influence of different tillage regimes and types of crop residue management on soil microbial communities. These studies were conducted under specific climates on soils characterized by particular land-use histories. A very different ecological context is to be found in certain areas of Western Europe, such as central Belgium, whose loess-derived soils are among the most fertile in the world and have long been used for intensive agriculture. Specific objectives were to determine diversity levels and changes in microbial community composition under different combinations of tillage regime (conventional vs. reduced) and crop residue fate (residue removal R- vs. residues left R+ on the field). As reduced tillage results in two contrasting zones (the first centimeters of soil are mixed each year, while the soil below remains unperturbed), we chose to perform the analysis at two depths: 0 to 5 cm and 15 to 20 cm. [less ▲]

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See detailProtein structure modeling using backbone chemical shifts
Wanko Nembot, Alexis Marius ULg; Damblon, Christian ULg

Poster (2015, December 01)

The knowledge of the tridimensional structure of a protein is essential to study its interactions and understand its mode of action. The Purpose of our work is to quickly and easily determine the ... [more ▼]

The knowledge of the tridimensional structure of a protein is essential to study its interactions and understand its mode of action. The Purpose of our work is to quickly and easily determine the structure of proteins using the backbone chemical shifts. Backbone chemical shifts data are NMR parameters that can be rapidly, easily and accurately measured. This parameter is very sensitive to the conformation of amino acids and is used to deduct the secondary structure (TALOS, RCI,...). We therefore plan to use backbone chemical shifts as constraints on dihedral angles to quickly and easily determine protein structure. Several « de novo » methods like CS-Rosetta , CS23D et CHESHIRE have been recently developed in this purpose. We will use proteins of different sizes for which, the structure (X-ray or NMR structure) and chemical shifts backbone are available for testing the three softwares. Knowing that each of these softwares predicted a large number of low energy models on the one hand, and that the deployment and use of these tools constitutes obstacles for users who are not experts in computer science on the other hand, our goal will be to develop a platform that can easily compare these three methods based on quality of the structure produced. [less ▲]

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See detailStudy of the Martian cold oxygen corona from the O I 130.4 nm by IUVS/MAVEN
Chaufray; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

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See detailRetrieval of Mars' Upper Atmospheric Composition using Dayglow Observations by IUVS on MAVEN
Evans; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

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See detailCarbon substrate utilization and microbial biomass in European forest soils are related to tree species diversity
Carnol, Monique ULg; Baeten, Lander; Bosman, Bernard ULg et al

Poster (2015, December)

Tree species influence biogeochemical cycling through element deposition (throughfall, litterfall), root decomposition and exudates, and through their influence on the microbial activities in the soil ... [more ▼]

Tree species influence biogeochemical cycling through element deposition (throughfall, litterfall), root decomposition and exudates, and through their influence on the microbial activities in the soil. Yet, the effect of mixing tree species on soil functioning is unclear, in particular concerning the microbial diversity and activity in soils. Here we synthesize results from the Exploratory Platform of the FunDivEUROPE project (http://www.fundiveurope.eu/). This network of 209 comparative plots covering tree diversity levels of 1 to 5 species was established in existing mature forests in 6 European regions. These six focal regions represent a gradient of major European forest types from boreal to Mediterranean forests. The aims of this study were to determine the soil microbial biomass and metabolic diversity of soil bacteria for these 6 European forest regions, presenting each a tree species richness gradient and to analyse the impact of tree species richness and the role of other controlling factors. We analysed the relation between tree species diversity, the proportion of coniferous tree species and soil factors (pH, soil organic carbon, water soluble carbon and nitrogen) and the carbon substrate utilisation pattern of soil bacteria (BIOLOG Ecoplate), soil microbial biomass (fumigation-extraction), hot water carbon and nitrogen in the forest floor and the upper mineral soil horizon (linear mixed models, GLM for multivariate abundance data, discriminant correspondence analysis). Mean values of microbial biomass carbon ranged from 3264 (Italy) to 8717 (Finland) mg kg-1 in the forest floor and from 465 (Italy) to 3748 (Finland) mg kg-1 in the mineral soil. Statistical models predicted microbial biomass to increase in both soil layers by 7-8% with each step increase in tree diversity. Increased proportion of conifers was linked to a decrease in the number of carbon substrates used by soil bacteria. The types of carbon sources used were dependent on region, proportion of conifers, soil pH and water-soluble carbon and nitrogen. [less ▲]

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See detailProbing the Martian Atmosphere with MAVEN/IUVS Stellar Occultations
Gröller; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

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See detailMars Ozone Mapping with MAVEN IUVS
Lefèvre; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

Detailed reference viewed: 14 (0 ULg)
See detailThe Two Types of Aurora on Mars as Observed by MAVEN's Imaging UltraViolet Spectrograph
Schneider; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

Detailed reference viewed: 31 (2 ULg)
See detailThe Hot Oxygen Corona of Mars: Observations by MAVEN IUVS
Deighan; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

Detailed reference viewed: 18 (2 ULg)
See detailMAVEN/IUVS Apoapse Observations of the Martian FUV Dayglow
Correira; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

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See detailTides in the Martian Atmosphere as Observed by MAVEN IUVS
Lo; IUVS team; Stiepen, Arnaud ULg

Poster (2015, December)

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See detailEFHC1/Myoclonin-1 modulates the post-translational modification of microtubules
Medard, Laurie ULg; Godin, Juliette; Coumans, Bernard ULg et al

Poster (2015, December)

Rationale: Juvenile myoclonic epilepsies (JME) are one of the most common forms of genetic generalized epilepsy. Genetic studies have shown that heterozygous mutations in EFHC1/Myoclonin1 are responsible ... [more ▼]

Rationale: Juvenile myoclonic epilepsies (JME) are one of the most common forms of genetic generalized epilepsy. Genetic studies have shown that heterozygous mutations in EFHC1/Myoclonin1 are responsible for 3-22% of JME cases worldwide. The Myoclonin1 protein contains three DM10 domains of unknown function and an EF-hand domain. We have previously demonstrated that Myoclonin1 is a microtubule-associated protein involved in cell division and radial migration during neocortex development. In cells, this protein co-localized with specific structures rich in microtubules (MTs) such as the centrosome, the poles of the mitotic spindle or the motile cilia but not with cytoplasmic MTs. This suggests post-translational modifications (PTM) of MTs may be important for the interaction between Myoclonin1 and MTs Methods: We co-expressed the different enzymes catalyzing PTM of MTs with Myoclonin1 in U2OS cell line, and then performed immunocytochemistry and western blot analysis. We next performed pulldown and luciferase complementation assays to test protein interaction Results: With one of these enzymes, we observed a strong increase in PTM in the presence of Myoclonin- 1.Interestingly, the effect is observed even when a DM10 domain alone is co-expressed with the enzyme, suggesting for the first time a role for this domain. This suggests that Myoclonin1 may interact with and modulate the activity of this enzyme. By using luciferase complementation assay and pull down experiments, we could demonstrate that both proteins interact. Conclusions: Our data suggest Myoclonin-1 modulates specific PTM of MTs. This is of prime importance for microtubule dynamic and notably for neuroblast precursor migration during neocortex development. This could be the mechanism that explains why pathological forms of myoclonin-1 may affect brain development. [less ▲]

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See detailSpatial variations in the concentrations of mercury and persistent organic pollutants in free-ranging bottlenose dolphins from South Florida
Das, Krishna ULg; Damseaux, France ULg; Heithaus, M.R. et al

Poster (2015, December)

The bottlenose dolphin (Tursiops truncatus) is an abundant top predator found in nearshore waters of South Florida including heavily developed and remote coastal waters. The objective of this study was to ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is an abundant top predator found in nearshore waters of South Florida including heavily developed and remote coastal waters. The objective of this study was to quantify total mercury (T-Hg) and persistent organic pollutant (POPs: NDL-PCBs, PBDEs, DDT, HCH, HCB, PCDD/Fs and DL-PCBs) levels in bottlenose dolphins found off the highly populated island of Key West (n = 27) and from the undeveloped Florida Coastal Everglades (FCE; n = 20). T-Hg and POPs were analyzed in skin and blubber tissues, respectively, using a Direct Mercury analyzer (for T-Hg), GC-ECD (POPs) and GC-HRMS (DLCs). The 7 ICES PCBs were the main compounds found in bottlenose dolphins from Key West (8229 ng.g-1 lipids) and the FCE (2289 ng.g-1 lipids), while the concentrations of PCDD/Fs remained low (Key West: 104 pg.g-1 lipids, FCE: 102 pg.g-1 lipids). POP concentrations were higher in individuals from Key West compared to those from the FCE. However, POP concentrations in Key West dolphins were lower than those from other locations in Florida and around the world. Unlike organic pollutants, T-Hg concentrations were significantly higher in FCE dolphins (Key West: 2941 ng.g-1 dw versus, FCE: 9314 ng.g-1 dw), with the highest concentrations reported from the southeastern US. PCB concentrations remained under previously suggested threshold for adverse health effects (including immunosuppression) in marine mammals of 17 000 ng/kg lipid. To conclude, sources of T-Hg and POPs differed between Key West and the FCE as reflected by their concentrations in skin and blubber of free-ranging bottlenose dolphins highlighting their role as sentinels of their environment. [less ▲]

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See detailCardio-pulmonary mechanics and minimal modelling in critical care
de Bournonville, Sébastien; Pironet, Antoine ULg; Desaive, Thomas ULg et al

Poster (2015, November 26)

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See detailForward Stagewise Additive Modeling for Computer Aided Diagnosis (CAD) Systems
Wehenkel, Marie ULg

Poster (2015, November 26)

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Peer Reviewed
See detailMiombo Woodlands : an endangered forest ecosystem in periurban areas of the southeastern cities of Democratic Republic of Congo
Hick, Aurélie ULg; Kizila Wimana, Pacifique; Tooth, Martin et al

Poster (2015, November 26)

Detailed reference viewed: 30 (1 ULg)
Peer Reviewed
See detailSleep deprivation affects brain global cortical responsiveness
Gaggioni, Giulia ULg; Ly, Julien; Chellappa, Sarah et al

Poster (2015, November 26)

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See detailBiomass and carbon stocks of tropical African forests: synthesis and perspectives
Loubota Panzou, Grâce Jopaul ULg; Doucet, Jean-Louis ULg; Loumeto, Jean-Joël et al

Poster (2015, November 26)

Quantifying the biomass and carbon stocks contained in tropical forests has become an international priority for the implementation of the REDD+ mechanism. Forest biomass is estimated through three ... [more ▼]

Quantifying the biomass and carbon stocks contained in tropical forests has become an international priority for the implementation of the REDD+ mechanism. Forest biomass is estimated through three successive levels: the tree, the stand and the region level. This study reviews the state of the art on the estimation of biomass and carbon stocks contained in tropical African forests. This review highlights that only few allometric equations, equations used for estimating biomass of the tree from non-destructive measurements (diameter, height), have been established for tropical African forests. At the stand level, this synthesis highlights the spatial and temporal variations in biomass between forests types in Central and Eastern Africa. While biomass recovery after disturbance (logging for instance) is rather quick, there is still a lot of uncertainity on the spatial variation in biomass, and there is no consensus on a regional biomass map. The quality of biomass mapping in tropical Africa stronly depends on the various sensors used (optical, RADAR or LIDAR), the allometric equation used to convert forest inventory data and sampling design. Based on the lack of precision of available allometric equations and forest inventory data a large spatial scale, many uncertainties persist on estimating the biomass and carbon stocks contained in the African tropical forests. It is important to develop reference sites (both allometry and forest inventory) to provide accurate biomass estimates for an effective implementation of the REDD+. [less ▲]

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See detailUne approche pour l'amélioration de la qualité au CHU de Liège.
LASRI, Saadia ULg; BASSLEER, Bernard ULg

Poster (2015, November 24)

Présentation du concept de déclaration informatisée d'événement indésirable.

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Peer Reviewed
See detailTrajet de soins et patient traceur pour la culture de qualité des soins et de sécurité des patients au CNRF.
BASSLEER, Bernard ULg; Jeunehomme, Laura; Guillaume, Daniel et al

Poster (2015, November 24)

Exposé méthodologique pour augmenter la culture organisationnelle de la qualité des soins dans un contexte d'accréditation hospitalière.

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See detailMultimedia-enhanced learning productions - A typology based on instructional use and needed resources
Van de Poël, Jean-François ULg; Martin, Pierre; Verpoorten, Dominique ULg

Poster (2015, November 24)

This poster introduces a typology of educational multimedia objects stemming from an analysis of 400 audiovisual items produced by IFRES-eCampus, the staff development unit of the University of Liège ... [more ▼]

This poster introduces a typology of educational multimedia objects stemming from an analysis of 400 audiovisual items produced by IFRES-eCampus, the staff development unit of the University of Liège (Belgium). The classification of the multimedia productions is based on their instructional use and is enriched by additional qualifications in terms of time investment by the faculty and the Teaching and Learning center. [less ▲]

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See detailDémarche participative des patients et Comité de patients au CHU de Liège.
BASSLEER, Bernard ULg; BOULANGER, Jean-Marie ULg; DOPPAGNE, Caroline ULg

Poster (2015, November 24)

Des éléments tels que l’évolution socio-économique, l’évolution des soins, la généralisation de l’accès à l’information pour le patient, les attentes des patients, la parution de différents articles de ... [more ▼]

Des éléments tels que l’évolution socio-économique, l’évolution des soins, la généralisation de l’accès à l’information pour le patient, les attentes des patients, la parution de différents articles de loi (en particulier la loi sur les droits du patient et certaines recommandations de l’OMS) amènent une évolution dans le rapport entre les soignants et les patients. La médecine évolue peu à peu vers un modèle de médecine participative. [less ▲]

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See detailUNRAVELLING THE ROLES OF LYSINE ACETYLATION BY ELP3 DURING INNER EAR DEVELOPMENT
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Freeman, Stephen ULg et al

Poster (2015, November 23)

We planned to investigate the role of Elp3 acetyl-transferase, a member of the Elongator complex, in inner ear formation. We first analysed the spatio-temporal pattern of Elp3 mRNA expression and showed ... [more ▼]

We planned to investigate the role of Elp3 acetyl-transferase, a member of the Elongator complex, in inner ear formation. We first analysed the spatio-temporal pattern of Elp3 mRNA expression and showed that it was expressed in the entire early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea, in the spiral ganglion and in the vestibule. To unravel functions of Elp3, we used conditional knock-out mice in which Elp3 gene is deleted from early otocyst (Elp3cKO). We submitted these mice to a battery of vestibular testing and found significant abnormalities. Besides, the auditory brain stem response of Elp3cKO indicated that these mice are severely deaf. We were also able to demonstrate an increased level of apoptosis in the Elp3cKO spiral ganglion leading to a reduced number of neurons and fibers innervating the sensory cells as well as a reduced number of their synaptic ribbons. Moreover, the remaining spiral ganglion neurons extend processes showing clearly defects regarding sensory cell innervation. In conclusion, our results clearly show a role for Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins that are targeted for acetylation by Elp3. [less ▲]

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See detailEffects of Plant Growth-Promoting Rhizobacteria on Wheat Growth under Greenhouse and Field Conditions in combination with different nitrogen fertilizer levels
Nguyen, Minh ULg; Ongena, Marc ULg; Colinet, Gilles ULg et al

Poster (2015, November 23)

Many Plant Growth-Promoting Rhizobacteria (PGPR) are able to enhance root growth, mineral availability, and nutrient use efficiency of crops. The aim of this project is to screen commercially available ... [more ▼]

Many Plant Growth-Promoting Rhizobacteria (PGPR) are able to enhance root growth, mineral availability, and nutrient use efficiency of crops. The aim of this project is to screen commercially available PGPR formulations and lab strains to increase wheat growth and yield in combination with an optimized nitrogen (N) fertilizer application scheme. This could lead to a significant reduction of N fertilizer application without affecting the subsequent grain yields. The screened products collection includes (1) Mix1 (a mix of Azospirillum sp., Azorhizobium sp., and Azoarcus sp.), (2) Mix2 (a mix of Mix1 complemented with two strains of phosphorus-solubilizing Bacillus sp.), (3) Bacillus amyloliquefaciens a, (4) B. subtilis, and (5) B. amyloliquefaciens b. These products were screened under greenhouse and field conditions in 2014 by using spring and winter wheat varieties, respectively. Under greenhouse conditions, there was a significant increase in root dry weight and in root per shoot ratio of plants inoculated with Mix1. Under field conditions, the interaction between PGPR inoculation and different N fertilizer doses was assessed. The grain yield was negatively impacted by low N fertilizer applications. Under such conditions, the inoculation of the wheat rhizosphere with B. subtilis increased the grain yield by 15% relative to the water control. However, in the field trial, the variability between plot replicates was high and lead to non-significant results. Based on these results, modified screening strategies for PGPR selection were set up for the next trials. [less ▲]

Detailed reference viewed: 149 (5 ULg)
See detailPegylated Polyplexes Based On HDAC5 siRNA And Aliphatic Polycarbonate Polymers For An Anticancer Therapy
Frère, Antoine ULg; Baroni, Alexandra; Peulen, Olivier ULg et al

Poster (2015, November 23)

Detailed reference viewed: 20 (10 ULg)
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See detailLIFE CYCLE ASSESSMENT OF ANTHROPIC WATER
Groslambert, Sylvie ULg; Léonard, Angélique ULg

Poster (2015, November 19)

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be ... [more ▼]

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be based on a continuous, objective and strict evaluation of the evolution of the state of environmental components (air, water, soil, biodiversity, etc.), the pressures to which they are subjected, the resultant impacts and the measures taken to improve living conditions within our Region. Within this framework, the General Operational Direction of Agriculture, Natural Resources and Environment (DGARNE) realises reports on the state of the environment in Wallonia, in close collaboration with universities and research centres. A chapter is dedicated to the efficient use of water resources, including a life cycle assessment of anthropic water and rain water harvesting. [less ▲]

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See detailPhénologie des hêtres dans une forêt ardennaise: comparaison de méthodes de suivi et relations avec les flux de CO2
Hurdebise, Quentin ULg; Vincke, Caroline; De Ligne, Anne ULg et al

Poster (2015, November 18)

Bien caractériser la phénologie des essences forestières dans un contexte climatique changeant est indispensable. Dans cette optique, confronter les différentes sources d’informations phénologiques ... [more ▼]

Bien caractériser la phénologie des essences forestières dans un contexte climatique changeant est indispensable. Dans cette optique, confronter les différentes sources d’informations phénologiques (relevé de terrain, modélisation, capteurs de rayonnement, caméra phénologique, satellite, eddy covariance…) est une démarche essentielle. Les différences et la complémentarité de ces sources d’informations ont été mises en évidence en utilisant les données provenant d’une forêt mixte de l’Est de la Belgique, principalement composée de hêtres. Par ailleurs, ces informations ont été utilisées pour étudier l’influence de la phénologie des hêtres sur les échanges annuels de CO2 de ces derniers. [less ▲]

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See detailComment améliorer la réussite à l'examen d'anatomie en 1ère année de bachelier en médecine? Etude de l'impact d'une activité intégrée dans un dispositif hybride de formation
Ernst, Denise ULg; SEIDEL, Laurence ULg; Defaweux, Valérie ULg et al

Poster (2015, November 17)

The course of “Introduction to Human Anatomy” is part of the curriculum delivered to the undergraduated students (Freshman year) at the Faculty of Medecine of the University of Liege. The course is taught ... [more ▼]

The course of “Introduction to Human Anatomy” is part of the curriculum delivered to the undergraduated students (Freshman year) at the Faculty of Medecine of the University of Liege. The course is taught in a blending learning mode combining traditional intructor-led and e-learning activities. The MCQ 2 test is one of the three components of the final written exam (which consists of 3 MCQ tests). This test requires students to recognize and identify the organ’s orientation in cross-sections represented in different spatial views. In our study, we tried to evaluate how the online training exercises provided by the instructors team for that particular ability was appreciated by students and if students benefited from this type of online activities to improve their examination results for MCQ 2 test. Further in our research, we asked student about their perceptions of coherence between the aims, methods and evaluation of our entire system. Then this perception has been compared with the overall score of the exam to look for the present of correlation elements. We observed that the MCQ 2 test results are significantly better for students who practiced the new online exercises and for students having seen the value of this activity. The perception of consistency or coherence between the objectives, methods and assessment is not correlated to the overall score of the student. As conclusion, we can declare that the new learning scenario proposed positively enriches the learning environment for students. It can be easily scaled up for important groups and accessed at any time by users. It focusses on specific objectives, is directly linked to the examination system of the course (MCQ 2) and also allows developing a competence expertise related to their future medical profession. [less ▲]

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See detailImpacts of Plant Growth-Promoting Rhizobacteria-based Biostimulants on Wheat Growth under Greenhouse and Field Conditions
Nguyen, Minh ULg; Ongena, Marc ULg; Colinet, Gilles ULg et al

Poster (2015, November 16)

Plant Growth-Promoting Rhizobacteria (PGPR) are one of the main biostimulant classes due to their capacity of stimulating root growth and enhancing soil mineral availability, hence increasing nutrient use ... [more ▼]

Plant Growth-Promoting Rhizobacteria (PGPR) are one of the main biostimulant classes due to their capacity of stimulating root growth and enhancing soil mineral availability, hence increasing nutrient use efficiency in crops. The aim of this study is to screen commercially PGPR-containing products to enhance wheat growth and yield in combination with an optimized nitrogen (N) fertilizer application scheme. This could lead to a significant reduction of N fertilizer application without affecting the subsequent grain yields. The screened products collection includes (1) Mix1 (a mix of Azospirillum sp., Azorhizobium sp., and Azoarcus sp.), (2) Mix2 (a mix of Mix1 complemented with two strains of phosphorus-solubilizing Bacillus sp.), (3) Bacillus amyloliquefaciens a, (4) B. subtilis, and (5) B. amyloliquefaciens b. These biostimulants were screened under greenhouse and field conditions in 2014 by using spring and winter wheat varieties respectively. There was a significant increase in root dry weight and in root per shoot ratio of plants inoculated with Mix1. Under field conditions, the interaction between PGPR inoculation and N fertilizer application was assessed. The grain yield was negatively impacted by low N fertilizer applications. Under such conditions, the inoculation of the wheat rhizosphere with Bacillus subtilis increased the grain yield by 15% relative to the water control. However, in the field trial, the variability between plot replicates was high and lead to non-significant results. Based on those results, modified screening strategies for PGPR selection were set up for the 2015 trials to reduce field variability and possibly achieve higher yield increases. [less ▲]

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Peer Reviewed
See detailEstetrol : a new natural estrogen providing a safe therapeutic window for the treatment of menopause
Gérard, Céline ULg; Gallez, Anne ULg; Silva, Elisabete et al

Poster (2015, November 14)

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would ... [more ▼]

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would provide useful advances for patient care. Estetrol (E4) is a liver friendly promising candidate for HRT. In preclinical and/or clinical studies, E4 has been effective against the main symptoms of menopause from a starting dose of 0.3 mg/kg/day. The aim of this study was to define the impact of E4 on mammary gland and breast cancer development. Our preclinical data show that E4 is less efficient than estradiol (E2) to induce mammary gland growth. Treatment with several concentrations of E4 has shown that E4 did not increase tumor development, when it is used at 0.3 mg/kg/day. However, at 3 mg/kg/day, E4 increased tumor growth similarly to E2 (0.08 mg/kg/day). E4 presents also some anti-estrogenic effects on mammary gland and antitumor activity on breast cancer by decreasing the strong proliferative effect of E2. While ERα is the predominant receptor mediating its effects, the dual weak-estrogenic/anti-estrogenic feature of E4 results from differential signaling pathways activation. Both nuclear and rapid extra-nuclear signaling pathways are necessary for a complete estrogenic effect of E4. However, the antitumor action of E4 is not due to a capacity to antagonize E2-induced nuclear activity. In conclusion, our results support that E4, if it is used in strictly controlled clinical applications, could have no or only limited impact on breast and breast cancer. [less ▲]

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See detailStructure of the Q-Codes semantic knowledge base in General Practice/Family Medicine
Jamoulle, Marc ULg; Grosjean, Julien; Darmoni, Stefan

Poster (2015, November 13)

Aim ; Coding abstracts of communications of GPs during congress allows more efficient exchange of information through a specific indexing process. Coding with Core Content Classification in General ... [more ▼]

Aim ; Coding abstracts of communications of GPs during congress allows more efficient exchange of information through a specific indexing process. Coding with Core Content Classification in General Practice/Family Medicine (3CGP/FM), an association of ICPC and of a new tool called Q-Codes means identifying the main concepts the writer is willing to share in his/her contribution. This is called ‘text classification’ or ‘topic list building’ in computer science and ‘qualitative data analysis’ in cognitive science. The GP is dealing with issues concerning the patient and with issues concerning the management of the job. Symptoms, diagnosis and process are what we will call clinical activities. General management as well as ethical or quality related issues are up to what we will call non-clinical or managerial activities. Clinical activities will be identified in the abstracts by the use of the International Classification of Primary Care second edition, electronic, version 5 (ICPC-2 e ver5). Managerial activities will be identified in the abstracts by the use of the Q-Codes, version 2.1, 2015 which we are explaining now in detail. Method ; www.hetop.eu is the European cross-lingual portal of health terminologies for indexing and searching Web resources. The portal uses OWL 2 Web Ontology Language. We have developed the terminological content of 183 items called Q-codes using a graphic interface, populating the knowledge base with relevant information about the concepts studied. These concepts are linked to: (i) professional terminologies such as MeSH, (ii) lay terminologies such as Babelnet and DBpedia, and (iii) top bibliographic free full text citations of the domain GP/FM. Results ; One hundred and eighty-three Q-Codes have been designed and 177 extensive terminological records are available online for now for beta testers. The whole process is described on http://docpatient.net/3CGP. The ongoing version of the Q-codes is available here in 6 languages http://docpatient.net/3CGP/Q-Codes_ver_2_2.pdf This poster present a general overview of the terminological development, showing the relations of the Q-Codes QT3, Quality Assurance [less ▲]

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See detailFROM METABOLOMICS STUDY OF AGE RELATED MACULAR DEGENERATION (AMD) TO THE DEVELOPMENT OF NEW PDK INHIBITORS
Arslan, Deniz ULg; Schoumacher, Matthieu ULg; Pirotte, Bernard ULg et al

Poster (2015, November 12)

Metabolomics is one of the most recent technologies in the Omics sciences defined as “the comprehensive characterization of small molecules (called metabolites) in different biological samples.” This ... [more ▼]

Metabolomics is one of the most recent technologies in the Omics sciences defined as “the comprehensive characterization of small molecules (called metabolites) in different biological samples.” This methodology can be applied in many areas, such as biomarker discovery, clinical studies, drug efficacy and toxicity evaluation, diagnostic tools, quality control or drug discovery. Its capability to extract biochemical information associated with a cellular or biological system makes this technique a powerful tool for Medicinal Chemistry. In this work, we present a 1H NMR metabolomics study applied to therapeutic target discovery. Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population of industrialized countries. This blindness results from the deterioration of the macula, a small part of the retina specialized for the high-acuity vision. Exudative AMD, called “wet”, is characterized by the formation of new blood vessels growing under the retina according to a process named choroidal neovascularization (CNV). Currently, the aetiology and pathogenesis of AMD remain unclear. Nevertheless, a recent metabolomics study performed on the serum of “wet” AMD patients and on a CNV murine model, that mimics the effect of “wet” AMD, have demonstrated that lactate level is clearly involved in the severity of the pathology as well as the relationship between lactate, CNV and AMD. According to this result, we suggest a new therapeutic approach of AMD based on the normalization of blood lactate level. The modulation of the lactate plasma concentration by treatment of the animals with synthetic compounds and more specifically Pyruvate Dehydrogenase Kinase (PDK) inhibitors significantly decrease the CNV. Starting from these results, development of new PDK inhibitors could open the way to innovative treatment opportunities in AMD disease. [less ▲]

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See detailIntégrer les acquis de la recherche en didactique dans la formation des enseignants en sciences.
Daro, Sabine; Graftiau, Marie-Christine; Hindryckx, Marie-Noëlle ULg

Poster (2015, November 10)

Comment rendre accessible les écrits des recherches en didactique en formation d'enseignants? Les formateurs d'enseignants utilisent-ils les écrits de la recherche? A quelles occasions? Si non, pourquoi ... [more ▼]

Comment rendre accessible les écrits des recherches en didactique en formation d'enseignants? Les formateurs d'enseignants utilisent-ils les écrits de la recherche? A quelles occasions? Si non, pourquoi? Résultats d'une enquête. [less ▲]

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See detailConduire un projet techno-pédagogique avec trois universités francophones belges
Vincke, Grégoire ULg; Marée, Raphaël ULg; Wehenkel, Louis ULg et al

Poster (2015, November 10)

L’imagerie numérique devient omniprésente dans beaucoup de métiers, dont ceux de la médecine. Sa montée en puissance questionne l’université quant à la meilleure manière d’entraîner les étudiants à lire ... [more ▼]

L’imagerie numérique devient omniprésente dans beaucoup de métiers, dont ceux de la médecine. Sa montée en puissance questionne l’université quant à la meilleure manière d’entraîner les étudiants à lire, interpréter, comparer, contextualiser et discuter ces ressources inédites. A l’université, ces compétences s’acquièrent principalement, aux premiers stades de la formation, dans les cours d’histologie. Dans ce contexte, l’IFRES a initié un projet wallon (Appel Germaine Tillion / Convention n°1318185 http://histoweb.ifres.ulg.ac.be ) dont l’objectif est la transformation de la plate-forme logicielle wallonne Cytomine – http://cytomine.be une solution Web existante d'échange, de visualisation, d'annotation et d’analyse collaborative semi-automatique d'images numérisées – en un système d’activités pédagogiques tirant parti des initiatives partielles existant chez les partenaires et des évolutions techniques les plus récentes. Le projet HistoWeb travaille à cette transformation en s’appuyant, au plan conceptuel, sur l’exploration de la notion d’« écologie d’apprentissage ». Le projet compte parmi ses partenaires 3 universités (ULB, UNamur, Liège) et exige donc de chercher un équilibre entre des développements techno-pédagogiques susceptibles de les intéresser tous et des développements spécifiques et en prise avec les écologies d’apprentissage propres à chaque institution. Le poster présente les manières choisies par l’IFRES pour coordonner l'ensemble du projet, travaillant tantôt individuellement tantôt collectivement avec les différents partenaires. Le poster aura un mot par ailleurs sur la collaboration entre pédagogues et développeurs informatiques. En effet, l’IFRES, sur toute la durée du projet est appelé à travailler avec une équipe de recherche (Systmod) dépourvue de familiarité avec les leviers de l’enseignement et de l’apprentissage. Il évoquera aussi les collaborations espérées d’un passage en Open source. Le poster pourra apporter des éclairages aux questions : - qu’entend-on précisément par collaboration lorsqu’il s’agit de mener de la « recherche » dans un contexte académique ? Il sera ici question de cet équilibre entre convergence et différentiation. - Qui est susceptible de se développer professionnellement ? Les bénéfices tirés du projet par les uns et les autres au niveau pédagogique seront ici évoqués. [less ▲]

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See detailCollaborer avec des enseignants-témoins en formation pédagogique d’adultes – Finalités, difficultés et plus-values
Leduc, Laurent ULg; Detroz, Pascal ULg; Verpoorten, Dominique ULg

Poster (2015, November 10)

En prélude à une étude empirique, ce poster problématise la décision pédagogique de faire intervenir, au cours d’une formation, des enseignants pour témoigner devant d’autres enseignants de leur pratique ... [more ▼]

En prélude à une étude empirique, ce poster problématise la décision pédagogique de faire intervenir, au cours d’une formation, des enseignants pour témoigner devant d’autres enseignants de leur pratique. Le poster recourt au QQOQCP (qui-quoi-où-quand-comment-pourquoi), une méthode qui allie simplicité et souci de complétude dans le balisage initial d’un phénomène. Sans remettre en cause la richesse des témoignages par des enseignants dans un contexte de formation, ni la satisfaction que beaucoup de participants estiment en retirer, le poster plaide pour une approche raisonnée des témoignages en soulignant certaines tensions et ambiguïtés qui peuvent marquer ces derniers. [less ▲]

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