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See detailRing-opening polymerization of cyclic phosphoesters
Vanslambrouck, Stéphanie ULg; Clement, Benoît ULg; Jérôme, Christine ULg et al

Poster (2012, November 16)

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See detailYttrium orthoferrite thin films... a great challenge
Stevens, Frédéric ULg

Poster (2012, November 16)

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See detailTherapeutic compounds in garlic oil: production and evolution after preparation
DETHIER, Bérénice ULg; Fauconnier, Marie-Laure ULg

Poster (2012, November 15)

Allyl sulfides, the main constituents of garlic oil, have been studied in the past decades for their effects against cancer. Since cancers are not all healed, ways to prevent it, especially with natural ... [more ▼]

Allyl sulfides, the main constituents of garlic oil, have been studied in the past decades for their effects against cancer. Since cancers are not all healed, ways to prevent it, especially with natural products that could be included in food preparation, are more than welcome. The synthesis and separation of allyl sulfides can provide larger amount of allyl sulfide than the extraction in order to make biological tests. Furthermore, the improvement of the synthesis conditions helps the understanding of the formation of allyl sulfide while garlic is processed. Finally, the study of the products allows a deeper understanding of the conversion between the allyl sulfides. A parallel can then be done with the conservation conditions of garlic-based products. The first part of this work describes the synthesis optimization using the design of experiment. The reaction of allyl bromide and sodium disulfide was carried under different conditions: the reaction duration, the heating (in microwave oven or in oil bath, at different temperature), the presence of catalyst, and the agitation were assessed. In the second part of the study, extractions are performed on garlic. The composition of the extracts is followed during time under the conditions established in the first part. The results give an optimized way to synthesize allyl sulfides, as much as an idea of the reactions happening on them in garlic preparations under various conditions. [less ▲]

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See detailUpwelling, filament and eddies in the Canary Current upwelling system during the CAIBEX cruise (Summer 2009)
Troupin, Charles ULg; Sangrà, Pablo; Arístegui, Javier et al

Poster (2012, November 15)

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See detailLa signification dans la représentation graphique du projet d'opération urbaine
Boutemadja, Abdelkader ULg

Poster (2012, November 15)

La signification dans la représentation graphique du projet d'opération urbaine

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See detailInfluence of cutting height on dry matter production and crude protein content of four Panicum maximum ecotypes in Benin (West Africa)
Adjolohoun, Sébastien; Bindelle, Jérôme ULg; Adandédjan, Claude et al

Poster (2012, November 15)

A field study was conducted under rainfall conditions (1200 mm/year) to determine the effects of different cutting heights (5, 10, 15, 20 cm) on dry matter production and fodder quality of four Panicum ... [more ▼]

A field study was conducted under rainfall conditions (1200 mm/year) to determine the effects of different cutting heights (5, 10, 15, 20 cm) on dry matter production and fodder quality of four Panicum maximum local ecotypes (HHLLLW, MHMLLW, lHSLNL, lHLLLW) differing in their morphology. Ecotype MHMLLW appeared more suitable and the best management is to harvest at 15 cm height. It produced 6054 kg DMyear-1 containing 8.05% CP. [less ▲]

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See detailNegative wind anomalies generated a diminution of productivity of in the North Atlantic in 2010
Troupin, Charles ULg; Machín, Francisco

Poster (2012, November 14)

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See detailUtilisation des termites comme source de microorganismes dans la filière de production du bioéthanol de seconde génération
Tarayre, Cédric ULg; Bauwens, Julien ULg; Brasseur, Catherine ULg et al

Poster (2012, November 14)

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres ... [more ▼]

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres fermentescibles libérés suite à cette hydrolyse sont utilisables dans le cadre de la production du bioéthanol de seconde génération. [less ▲]

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See detailViandes bovines à longue durée de conservation conditionnées sous vide : isolement et caractérisation de souches de Carnobacterium
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Baptista Rodrigues, Ana Lucia ULg et al

Poster (2012, November 13)

The lactic acid bacteria Carnobacterium divergens and Carnobacterium maltaromaticum are often associated to meat and meat products and may be used as a protective culture, improving the microbial ... [more ▼]

The lactic acid bacteria Carnobacterium divergens and Carnobacterium maltaromaticum are often associated to meat and meat products and may be used as a protective culture, improving the microbial stability and the safety of these products. In this context, the aim of this study was to isolate and characterize Carnobacterium from long shelf-life vacuum-packed beef. LAB counts after culture at +22°C remained below 2.0 log UFC/cm², even at the end of shelf life. On the other hand, the ecosystem evaluation performed by metagenomics revealed the predominance of Carnobacterium and Lactobacillus on the samples. After spreading of a peptone water suspension obtained from the samples on PCA, pure isolates were collected and identified by API 50 CHL galleries. Seventy-eight % of isolates were C. maltaromaticum, 3 % C. divergens and 19 % could not be identified. The next step of this work will consist in performing a genotypic and functional characterization of these Carnobacterium isolates. [less ▲]

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See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Poster (2012, November 13)

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See detailHybrid Lens for Solar Concentration: Optimization of the Multilayer Diffractive Lens
Languy, Fabian ULg; Habraken, Serge ULg

Poster (2012, November 12)

Manufacture process degrades the ideal shape of multilayer diffractive lens due to draft angle, half radius tool and slope error. We show some shape improvements using an extended scalar theory.

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See detailCan satellites help organic crop certification?
Denis, Antoine ULg; Desclee, Baudouin; Migdall, Silke et al

Poster (2012, November 08)

Organic agriculture, while producing healthy food and contributing to protect the environment, needs to be certified in order to meet the consumers confidence. The objective of this study was to ... [more ▼]

Organic agriculture, while producing healthy food and contributing to protect the environment, needs to be certified in order to meet the consumers confidence. The objective of this study was to investigate how earth observation techniques could enhance the crop certification process and in particular the possibility to discriminate organic and conventional fields. These different crop management methods results in crop biophysical differences which are supposed to be observable by earth observation techniques. A set of satellites with varying spatial and spectral resolution was used to compute discriminant indicators. Under the best conditions the results show a complete discrimination between organic and conventional crops. [less ▲]

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See detailCerebral aspergillosis in immunocompromised patient successfully treated with voriconazole
HUWART, Aline ULg; RADERMACHER, Jean; HENROTEAUX, Adrienne ULg et al

Poster (2012, November 08)

Cerebral aspergillosis is a severe invasive mycosis occurring in immunocompromised patients. This pathology is associated with a high rate of mortality and is a current complication of pulmonary invasive ... [more ▼]

Cerebral aspergillosis is a severe invasive mycosis occurring in immunocompromised patients. This pathology is associated with a high rate of mortality and is a current complication of pulmonary invasive aspergillosis. We report the case of a 44-year-old immunocompromised male with a recent history of oropharyngeal carcinoma. At his admission the patient presented with fever and confusion. Imaging revealed the presence of a cerebral abscess combined with lung infiltrates. During hospitalization and despite a broad-spectrum antibiotic regimen his condition worsened. A thin needle aspiration of the abscess was performed for diagnosis purpose. Histological examination of the tissue showed septate and branched hyphae with 45° angles suggestive of Aspergillus. A real-time PCR specific for the detection of Aspergillus sp. was carried out and confirmed the fungal etiology of the abscess. Rare colonies of A. fumigatus were isolated a few days later. The diagnosis of invasive pulmonary aspergillosis complicated by a cerebral dissemination was confirmed. Antifungal treatment based on voriconazole 4 mg/kg q12h was introduced and the dosage was successfully increased up to 5 mg/kg q12h by drug monitoring. This case highlights the usefulness of the Aspergillus PCR for the rapid identification of hyphae in tissue biopsies (or in the event of negative culture), and the importance of therapeutic drug monitoring in treatment by voriconazole. [less ▲]

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See detailEpuration catalytique de biogaz
Claude, Vincent ULg; Toye, Dominique ULg; Heinrichs, Benoît ULg et al

Poster (2012, November 08)

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See detailUse of Life Cycle Assesment to determine the environmental impact of thermochemical conversion routes of lignocellulosic biomass: state of the art.
Gerbinet, Saïcha ULg; Léonard, Angélique ULg

Poster (2012, November 07)

Abstract: The biomass is a promising way to substitute fossil fuels. Lignocellulosic biomass valorisation is part of second generation technologies. They are interesting in that they imply less ... [more ▼]

Abstract: The biomass is a promising way to substitute fossil fuels. Lignocellulosic biomass valorisation is part of second generation technologies. They are interesting in that they imply less competition with food crops for land and water, and they allow for the whole plant to be processed. Moreover, lignocellulose is abundant in cheap and non-food materials extracted from plants such as wood and energy crops. The thermo-chemical route is being considered more extensively, especially the gasification process. This process converts carbonaceous biomass into combustible gases (CO, H2, CO2, CH4, and impurities) called syngas in the presence of a suitable oxidant. The syngas can be converted into a large range of products, such as diesel, via a Fischer-Tropsch process, or methanol, used for producing DME (dimethyl ether), both of which can serve as fuels in traditional motors. Syngas can also be used to produce ethylene and propylene, two building blocks for the chemical industry. Production of these four compounds is specifically investigated. In order to insure that, under the principle of sustainability, the use of lignocellulosic biomass is a viable alternative, its environmental impact must be accurately quantified. The Life Cycle Assessment (LCA) methodology will be used in this regard for the gasification process. The gasification technology will be described, and a state of the art in LCA of the gasification process will be presented. Finally, the need for new research will be established. [less ▲]

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See detailPerformance Evaluation of the GE eXplore CT 120 Micro-CT for Various Scanning Protocols
Bahri, Mohamed Ali ULg; Bretin, Florian ULg; Warnock, Geoffrey ULg et al

Poster (2012, November 03)

The aim of this study was to evaluate the performance of the General Electric (GE) eXplore CT 120 micro-CT using the same methodology and image quality assurance vmCT phantom developed for the GE eXplore ... [more ▼]

The aim of this study was to evaluate the performance of the General Electric (GE) eXplore CT 120 micro-CT using the same methodology and image quality assurance vmCT phantom developed for the GE eXplore Ultra. In addition, Quality assurance in Radiology and Medicine (QRM) low contrast and bar pattern phantoms were used. The phantoms were imaged using the six protocols regularly used in our laboratory (Fast scan 220 (P1) or 360 (P2): 70 kV, 32 mA, 220 or 360 views; Soft tissue fast scan (P3): 70 kV, 50 mA, 220 views, Soft tissue step & shoot (P4): 80 kV, 32 mA, 220 views; Low Noise (P5): 100 kV, 50 mA, 720 views and In Vivo Bone scan (P6): 100 kV, 50 mA, 360 views). Data were reconstructed with an isotropic voxel size of 100 µm (50 µm when protocol detector-binning was reduced to 2x2). The MTF obtained with the slanted edge and coil methods agreed very well. A 10% modulation transfer function (MTF) was observed in the range 3.6-4.8 mm-1 (P1&2 = 4.2; P3&4 = 4.8; P5 = 3.6 and P6 = 3.8), corresponding to 95-138 µm resolutions. The smallest bars visually observed on the QRM pattern phantom image were 100 µm. The geometric accuracy was better than 0.1%. A highly linear (R2 > 0.999) relationship between measured and expected CT numbers for both the CT number accuracy and linearity sections of the phantom was observed with a voltage dependent slope. A cupping effect was observed on the uniform slices. This effect was clearly highlighted by the uniformity-to-noise ratio (P1 = 0.58, P2&3&4 = 0.75, P5 = 1.35 and P6 = 2.74) especially for the low-noise protocols P5 and P6. The best low contrast discrimination was observed for P2 and P5 protocols. In conclusion the eXplore CT 120 achieved a resolution in the range 95-138 µm. It was found to be linear and geometrically accurate. The major difference between the protocols was the noise level which limits the detectability of low contrasts. [less ▲]

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See detailMODELING OF A GLASS WOOL PROCESS IN VIEW OF LIFE CYCLE ASSESSMENT (L.C.A.)
Gerbinet, Saïcha ULg; Renzoni, Roberto ULg; Briard, Vincent et al

Poster (2012, November)

Taking into account the environmental aspects in the building sector has become unavoidable. In France, environmental and sanitary statements for building products (“Fiches de Déclarations ... [more ▼]

Taking into account the environmental aspects in the building sector has become unavoidable. In France, environmental and sanitary statements for building products (“Fiches de Déclarations Environnementales et Sanitaires” (FDE&S)) have been developed. The environmental part of the statement is based on Life Cycle Assessment. So, KnaufInsulation, glass wool producer for the French market, has started to evaluated the environmental impacts of it process in view of FDE&S realization. The process has been modeling in GaBi with industrial data. Adjustable parameters have been introduced to allow to study the environmental impacts of almost all the KnaufInsulation products. So the FDE&S can be easily done for the different products. This model is also used for eco-conception. The LCA results allow to show the life cycle leaks. More, in modifying the model, the impact of a change in the process on its environmental performances will be highlighted. So relevant improvement will be brought out. The model and the mains results as the eco-conception strategy will be presented. The interest of making a modeling based on the step and process of the life cycle of a product or a product range will be clearly understood. [less ▲]

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See detailVariable Renewable Energy integration and the balancing challenge: Flexibility assessment in Belgium
Quoilin, Sylvain ULg; Dewallef, Pierre ULg; Verbruggen, Aviel

Poster (2012, November)

The world is facing a historical increase in energy demand and energy consumption. Variable Renewable Energy (VRE) sources are considered as a solution to both environmental issues and energy demand, even ... [more ▼]

The world is facing a historical increase in energy demand and energy consumption. Variable Renewable Energy (VRE) sources are considered as a solution to both environmental issues and energy demand, even though they present high variability and low predictability. Variability and uncertainty are familiar aspects of all power systems. However, the increase of the share of renewable sources leads to new needs in terms of flexible resources. These resources can be provided by four different means: 1. Dispatchable power plants (i.e. with ramp up and ramp down capabilities) 2. Storage systems, mainly in the form of pumping stations. 3. Grid interconnections between countries 4. Demand side management. The present work focuses on the Belgian transmission grid. In order to assess its flexibility, both the technical flexible resource available and the flexibility requirement are evaluated in the considered area and the Present Variable Renewable Energy penetration Potential is calculated. [less ▲]

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See detailESR spectroscopy for the study of an inflammation-induced AKI cellular model
Quoilin, Caroline ULg; Mouithys-Mickalad, Ange ULg; Gallez, Bernard et al

Poster (2012, November)

The kidney is faced to an impairment of oxygen extraction during sepsis which is well-known to be a risk factor for the development of acute kidney injury (AKI). Recent research activities in the ... [more ▼]

The kidney is faced to an impairment of oxygen extraction during sepsis which is well-known to be a risk factor for the development of acute kidney injury (AKI). Recent research activities in the mechanisms involved in the development of AKI in sepsis emphasize the central role of hemodynamic and inflammatory events. More particularly, two mechanisms are suggested to explain the inability of the injured kidney to extract oxygen: tissue hypoxia and cellular energetic metabolism dysfunction. Our working hypothesis of the pathophysiology of AKI is based on cellular respiratory dysfunction due to the inflammatory response inherent to sepsis. To study the mechanism of oxygen regulation in inflammation-induced acute kidney injury, we investigate the effects of a bacterial endotoxin (lipopolysaccharide, LPS) on the basal respiration of proximal tubular epithelial cells (HK-2) by ESR oximetry. This method has shown that HK-2 cells exhibit a decreased oxygen consumption rate when treated with LPS. Surprisingly, this cellular respiration alteration persists even after the stress factor was removed. We suggested that this irreversible decrease in renal oxygen consumption after LPS challenge is related to a pathologic metabolic down-regulation such as a lack of oxygen utilization by cells. This decrease was accompanied by increased nitric oxide (NO) production as measured by a spin trapping technique using ESR spectroscopy. This method is based on the trapping of NO by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+) forming a water-soluble NO-FeMGD complex detected by ESR. Since inducible NO synthase (iNOS) has been shown to play an important role in sepsis-induced AKI, the iNOS inhibitor L-NMMA (L-NG-monomethyl Arginine citrate) was tested in this in vitro model. L-NMMA blocked NO generation and permitted the HK-2 cells to recover a normal cellular respiration. Overall, ESR spectroscopy and the model of HK-2 cells exposed to LPS displays some key features of inflammation-induced acute kidney injury. [less ▲]

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See detailWhat is the best use of sugar crops? Environmental assessment of two applications : biofuels vs. bioproducts
Belboom, Sandra ULg; Léonard, Angélique ULg

Poster (2012, November)

Agricultural crops became through years a possibility to increase European energy independence. Brazil has taken this opportunity since the seventies by using sugar cane bioethanol as vehicle fuel. The ... [more ▼]

Agricultural crops became through years a possibility to increase European energy independence. Brazil has taken this opportunity since the seventies by using sugar cane bioethanol as vehicle fuel. The development of biofuels production is more recent in Europe. Due to temperate climates, bioethanol production is mostly based on wheat and sugar beet, this latter being considered as the ‘equivalent’ sugar crop to sugar cane for Europe. Biofuel is the most common application of bioethanol but its transformation into bioethylene through a dehydration step can be an alternative as already found in Brazil. This paper will consider both potential uses and compare them using Life Cycle Assessment methodology. Common boundaries of the systems comprise the cultivation step for both crops, i.e. sugar cane and sugar beet, with all associated energetic and fertilizer consumptions, the transportation step from field to the industrial plant, the sugar crops transformation into hydrate bioethanol and the by-products valorisation. For the biofuel scenario, a dehydration step using molecular sieve is added to get anhydrous bioethanol. For the bioethylene scenario, an industrial dehydration step is added. Direct comparison between both scenarios is not possible due to different products uses. The comparison was then performed for both scenarios between the bio-based product and its fossil equivalent. ReCiPe 2008 method was used to get the environmental impacts. As expected, the impact of bio-based products in climate change and fossil fuel depletion categories decreases compared to the fossil counterparts. For other categories, difference is less significant and results are often better for fossil products. Land use change category was implemented to assess its importance. Depending on assumptions, the greenhouse gas emissions from crop implementation on a natural land can counteract the previous mentioned benefits. This study shows the importance of assumptions, especially in the agricultural field, on the obtained results. [less ▲]

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See detailMorbidity and Mortality of the "Late Preterm" Infants (Gestational Age : 34-366/7 Weeks)
MITSIAKOS, Georgios; GKIOUGKI, Evangelia ULg; TSEPIS, Konstantinos et al

Poster (2012, November)

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See detailCO2 CAPTURE in POWER PLANTS: Process Simulation and Solvent Degradation
Léonard, Grégoire ULg; Lepaumier, Hélène; Thielens, Marie-Laure et al

Poster (2012, November)

Presentation of the research themes studied at the University of Liège in the field of CO2 capture

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See detailTraitement orthodontique chez le patient adulte assisté par piezocision : Illustration d'un cas clinique
CHARAVET, Carole ULg; LECLOUX, Geoffrey ULg; LAMBERT, France ULg et al

Poster (2012, November)

La durée des traitements orthodontiques chez l’adulte reste une barrière pour certains d’entre eux. La littérature scientifique récente qui décrit la technique de piezocision impliquant des corticotomies ... [more ▼]

La durée des traitements orthodontiques chez l’adulte reste une barrière pour certains d’entre eux. La littérature scientifique récente qui décrit la technique de piezocision impliquant des corticotomies à minima semble être intéressante pour traiter ce type de patient. Cette communication décrit le cas clinique d’une patiente de 20 ans présentant une classe I bilatérale, un encombrement des fronts avec les canines supérieures en vestibulomésioinfraclusion et une ligne médiane inférieure déviée à droite. Une planification multidisciplinaire est indispensable pour traiter ce genre de cas. La technique chirurgicale nécessite une anesthésie locale. Des incisions verticales à minima sont réalisées en vestibulaire, de part et d’autre des dents à déplacer. Ensuite, par cette petite incision, une corticotomie inter-radiculaire de 5mm de long est réalisée à l’aide d’un piezotome. Il n’est pas nécessaire de suturer. Un appareil fixe multiattache est posé 1 semaine avant la procédure chirurgicale et le patient est convoqué tous les 15 jours activé. Dans ce cas, un appareil fixe bimaxillaire auto-ligaturant a été placé pour une friction minimale. Les suites post-opératoires pour le patient ont été minimes et la durée totale du traitement orthodontique a été de 7 mois. Le traitement dans sa globalité semble donc avoir été plus rapide qu’un traitement classique. Cependant, même si des études animales semblent confirmer le sentiment clinique décrit dans ce cas, des études cliniques contrôlées randomisées sont nécessaires pour valider cette approche de traitement. [less ▲]

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See detailSocio-ecological study of recreational boating in a Mediterranean marine protected area
Pérez-Perera, Amanda; Panzalis, Pier; Navone, Augusto et al

Poster (2012, November)

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See detailEvolution of the theoretical power spectrum of solar-like oscillations along the ascending phase on the red giant branch.
Grosjean, Mathieu ULg; Montalban Iglesias, Josefa ULg; Samadi, Reza et al

Poster (2012, November)

CoRoT and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. An important question comes from the observation of mixed ... [more ▼]

CoRoT and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. An important question comes from the observation of mixed modes : When during the star’s ascension on the RGB are mixed-modes more likely to be detectable ? We follow the evolution of a star on the RGB and investigate the effect of its ascension on theoretical power spectrum. Equilibrium models (computed with the code ATON) represent four different stages of a star on the RGB. The mass of the star (1.5M") is in the typical mass range of stars observed by CoRoT and Kepler. We used a non-radial non-adiabatic code to compute the theoretical solar-like oscillations of these models. An important output of these calculations is the theoretical lifetimes of the modes. Then we computed the oscillation amplitudes through a stochastic excitation model. These computations allow us to draw theoretical power spectrum and discuss the possibility to observe mixed-modes at different evolutionary stages on the RGB. We found that structure modifications in a star ascending the RGB have an important impact on theoretical power spectrum of solar-like oscillations. Efficiencies of trapping and lifetimes of mixed modes are indeed strongly affected by this evolution. [less ▲]

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See detailDevelopment of Highly Active doped Titania Photocatalysts by Aqueous Sol-Gel Processing
Malengreaux, Charline ULg; Douven, Sigrid ULg; Poelman, Dirk et al

Poster (2012, November)

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See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULg; Waroux, Olivier ULg; Cloquette, Karine et al

Poster (2012, November)

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS (expression rate: 90-99%). RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. Colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. [less ▲]

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See detailIdentification modale de systèmes variant dans le temps à partir de réponses simulées sur un exemple d'éoliennes
Bertha, Mathieu ULg; Golinval, Jean-Claude ULg

Poster (2012, November)

Wind turbines are good examples of time-varying systems as their modal properties depend on their instantaneous configuration. To catch the variations of modal parameters in time-varying systems ... [more ▼]

Wind turbines are good examples of time-varying systems as their modal properties depend on their instantaneous configuration. To catch the variations of modal parameters in time-varying systems, classical identification methods have to be adapted to the non-stationary nature of the recorded signals. In this paper, it is proposed to study the dynamic behavior of an offshore five-megawatt wind turbine. First, a numerical model of the wind turbine is created to serve as reference. Then, the time-varying behavior of the system is evaluated by simulating a large number of possible configurations. To this purpose, time responses are generated from the numerical model submitted to different environmental conditions. The wind is considered as the main non-measured external excitation force on the structure and the responses are recorded at several locations to simulate a real measurement process. Special care is brought to the accessibility of the measurement locations and to the limited number of available sensors in practice. Using these simulated measurements, output-only identification methods are used to extract varying dynamic properties of the structure. The final objective of this work is to pave the way to online condition monitoring of wind turbines. [less ▲]

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See detailInvestigation of eighteenth-century Prussian blue pigments by PDF analysis
Samain, Louise ULg; Martinetto, Pauline; Bordet, Pierre

Poster (2012, October 30)

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely ... [more ▼]

Prussian blue, a hydrated iron(III) hexacyanoferrate(II) complex, is a synthetic pigment discovered in Berlin in 1704. Because of both its highly intense color and its low cost, Prussian blue was widely used as a pigment in paintings until the 1970's. The early preparative methods were rapidly recognized as a contributory factor in the fading of the pigment [1], a fading already known by the mid-eighteenth century. The eighteenth-century methods are based on the calcination of dried blood to produce a potassium hexacyanoferrate complex, which is the first of two essential reactants for synthesizing Prussian blue. The second reactant is an iron salt. We successfully reproduced two typical eighteenth-century empirical recipes [2]. The resulting pigments were of variable color quality, ranging from intense blue to blue-gray or blue-green, and exhibit broadened or inexistent Bragg peaks. High-energy powder X-ray diffraction experiments were performed at the ID11 beamline at ESRF, Grenoble, France. The pair distribution function (PDF) of the pure Prussian blue pigments was refined with a three-phase model, in order to take into account the vacancy distribution in the unit cell of Prussian blue. In certain ancient Prussian blues, the PDF analysis revealed the presence of nanocrystalline ferrihydrite, Fe10O14(OH)2, and also identified the presence of alumina hydrate, Al10O14(OH)2, with a particle size of ca. 15 Å. Paint layers prepared from these ancient pigments subjected to accelerated ageing showed a tendency to turn green, a tendency that was often reported in eighteenth- and nineteenth-century books. The presence of particles of hydrous iron(III) oxides was also observed in a genuine Prussian blue sample obtained from an eighteenth-century polychrome sculpture. [1] Kirby, J.; Saunders, D. The National Gallery Technical Bulletin 2004, 25, 73. [2] Dossie, R. The Handmaid to the Arts; Nourse, J.: London, 1758; Le Pileur d'Apligny, M. Traité des couleurs matérielles et de la manière de colorer relativement aux différents arts et métiers; Saugrain et Lamy: Paris, 1779. [less ▲]

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See detailDoes processing speed protect from age-related decline in cognitive control ?
Manard, Marine ULg; Carabin, Delphine; Collette, Fabienne ULg

Poster (2012, October 27)

Age-related difficulties have been reported on proactive control whereas reactive control seems to remain intact (Braver, Gray, & Burgess, 2007; Braver, 2012). This study investigated the potential ... [more ▼]

Age-related difficulties have been reported on proactive control whereas reactive control seems to remain intact (Braver, Gray, & Burgess, 2007; Braver, 2012). This study investigated the potential influence of speed of processing abilities on the age-related decline in proactive control. We used a working memory recognition paradigm involving proactive or reactive cognitive control by manipulating the interference level across items. 80 young adults (18-29 years old) and 80 healthy older adults (60-89 years old) were included. The main results revealed significant effects of age on sensitivity to interference. As expected, reactive control performance remained intact with aging (similar interference effect in the two groups). In contrast, we observed a larger interference effect in the proactive condition in aging. Finally, when the groups are matched according to their processing speed (assessed by the Code task of the WAIS III, with both younger and older adults having a score comprised between 60 and 93), the effect of age on sensitivity to interference disappeared. In other words, when younger and older adults had similar speed of processing abilities, no age-related proactive control decline was observed. In conclusion, beyond the fact that this study confirms the selective age-related decline in proactive control, it also indicates that speed of processing, a measure considered as reflecting the integrity of cognitive functioning during aging (Salthouse, 1996), influences the efficiency of proactive control in that population. [less ▲]

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See detailMemories of Near-Death experiences are they memories of imagined events?
Thonnard, Marie ULg; Charland-Verville, Vanessa ULg; Brédart, Serge ULg et al

Poster (2012, October 27)

Background: The phenomenon of Near-Death Experiences (NDEs) has always intrigued but is still not fully explained despite numerous theories and studies. Since reports of NDEs are proposed to be imagined ... [more ▼]

Background: The phenomenon of Near-Death Experiences (NDEs) has always intrigued but is still not fully explained despite numerous theories and studies. Since reports of NDEs are proposed to be imagined events (French, 2001), and since memories of imagined events have, on average, fewer phenomenological characteristics than real event memories (e.g. Johnson et al., 1988), we here compared phenomenological characteristics of NDEs reports with memories of imagined and real events. Methods: We included 3 groups of coma survivors (8 patients with NDE as defined by the Greyson NDE scale – the “NDE memory group”- , 6 patients without NDE but with memory of their coma – the “coma memory group” – and 7 patients without memories of their coma – the “no memory group”) and a group of 18 age-matched healthy volunteers. Five memories were assessed using Memory Characteristics Questionnaire (MCQ – Johnson et al., 1988): target memory (NDE for NDE memory group, coma memory for coma memory group, and first childhood memory for no memory and control groups), old and recent real event memories and old and recent imagined event memories. Results: In NDE group, NDE memories showd more characteristics than memories of imagined and real events (p<0.02). These memories contain more self-referential and emotional information and have better clarity than memories of coma (all p<0.02). Conclusion: The present study showed that NDE memories contain more characteristics than real event memories and coma memories. Thus, they cannot be considered as classic imagined event memories. On the contrary, their physiological origins could lead them to be really perceived although not lived in the reality. Further work is needed to better understand this phenomenon [less ▲]

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See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULg; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, October 27)

Detailed reference viewed: 7 (1 ULg)
See detailComputation of photoelectron spectra and evidences of SAMO in C60 and C70
Mignolet, Benoît ULg

Poster (2012, October 26)

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See detailCambios ambientales del último milenio en sedimentos lacustres del Lago Thompson, Patagonia Chilena Norte
Sacré, Vincent ULg; Fagel, Nathalie ULg; Leclercq, Louis ULg et al

Poster (2012, October 25)

Este estudio se enfoca en un análisis multiproxy de un testigo sedimentario del Lago Thompson (area 1 km2; 751 masl ; 45°38’S 72°47’W) obtenido en 2008 (LT08-E, 130 cm), con el objetivo de documentar la ... [more ▼]

Este estudio se enfoca en un análisis multiproxy de un testigo sedimentario del Lago Thompson (area 1 km2; 751 masl ; 45°38’S 72°47’W) obtenido en 2008 (LT08-E, 130 cm), con el objetivo de documentar la variabilidad climática durante el último milenio en la Patagonia Chilena Norte y su impacto sobre el ambiente. El modelo de datación se basó en cinco fechados de 14C. Los resultados de 210Pb y 137 Cs no permitieron estimar la tasa de sedimentación para la parte superficial del núcleo. El lago Thompson esta caracterizado por una pequeña cuenca de suaves pendientes y sus sedimentos están dominados por limo arcilloso y partículas amorfas (principalmente diatomeas). Esto último es sustentado por la relación atómica de carbono/nitrógeno (C/N) que indica el origen fitoplanctónico del sedimento (8-10). Los perfiles de rayos –X, susceptibilidad magnética y LOI 550ºC evidencian dos depósitos volcánicos en 1410-1440 y 1780-1790 AD. Los perfiles de C/N y sílice biogénica (SiB) sugieren una alta productividad durante un periodo equivalente a la Pequeña Edad del Hielo (1650-1850 AD), con una máxima intensidad durante ~1800 AD. Desde 1850 AD hasta la actualidad, los perfiles de LOI 550ºC y SiB evidencian una reciente eutrofización y aumento del nivel del lago (asociado con aumento de temperaturas y humedad). Además, los perfiles de SiB presentan una correlación inversa con las temperaturas reconstruidas por anillos de árboles en la Patagonia. El contenido de diatomeas es dominado por especies planctónicas (Aulacoseira sp. y Cyclotella sp.), mientras que taxa bentónicos no muestran una buena diversificación en comparación a otros lagos de la Patagonia. La abundancia de diatomeas muestra respuestas específicas a los depósitos volcánicos con una disminución de Aulacoseira sp.. La relación planctónica/bentónica permite identificar dos periodos de alto y bajo nivel del lago coincidiendo con fases húmedas y secas de la Pequeña Edad de Hielo (1200-1650 y 1650-1850 AD). La realización de análisis sedimentológicos, geoquímicos y de microfósiles permitiría una mejor caracterización de los modelos de depositación del sedimento (impregnación del núcleo sedimentario) y de la interacción entre depósitos volcánicos y diatomeas. Agradecimientos a Fondecyt Nº1070508, FNRS 1360 2007-2010 y ULg CFRA 1060 2009-2010. [less ▲]

Detailed reference viewed: 32 (9 ULg)
See detailLes femmes chefs d'entreprises au Maroc
Salman, Noura ULg; El Abboubi, Manal; Henda, Sana

Poster (2012, October 24)

Cette étude qualitative descriptive de type exploratoire a pour but d’étudier la situation des femmes chefs d’entreprises au Maroc, à travers différents indicateurs: leur profil, les caractéristiques de ... [more ▼]

Cette étude qualitative descriptive de type exploratoire a pour but d’étudier la situation des femmes chefs d’entreprises au Maroc, à travers différents indicateurs: leur profil, les caractéristiques de leurs entreprises, leur style de gestion, leur choix de secteur, leur mode de financement ainsi que leur implication dans les réseaux professionnels. De même, d’autres éléments ont été analysés notamment au niveau des difficultés rencontrées en tant que femmes actives au sein d’une société caractérisée par certains facteurs contextuels spécifiques. Ces difficultés peuvent constituer un handicap sérieux face au développement de l’entrepreneuriat féminin au Maroc. Des entrevues semi-dirigées ont été réalisées auprès de 20 femmes chefs d’entreprises actives dans divers secteurs d’activité. Les résultats montrent que les femmes entrepreneures marocaines ont leur particularité. Celle-ci est souvent engendrée par des facteurs socioculturels liés à la société marocaine. [less ▲]

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See detailTo heal or not to heal: a multiscale model of the influence of oxygen during bone fracture healing.
Carlier, Aurélie ULg; Geris, Liesbet ULg; Van Oosterwyck, Hans

Poster (2012, October 24)

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See detailLa Régénération Naturell e Assistée, un outil efficace pour endiguer la savanisation des forêts galeries du plateau Batéké en RD Congo ?
Peltier, Régis; Perroches, Adrien; Marquant, Baptiste et al

Poster (2012, October 24)

L’agriculture itinérante sur brûlis et l’extraction du bois énergie sont les principales causes de dégradation des forêts d’Afrique Centrale. La Régénération Naturelle Assistée (RNA) des jeunes arbres ... [more ▼]

L’agriculture itinérante sur brûlis et l’extraction du bois énergie sont les principales causes de dégradation des forêts d’Afrique Centrale. La Régénération Naturelle Assistée (RNA) des jeunes arbres dans les parcelles cultivées, un outil de gestion appliqué avec succès depuis plus de vingt ans au Sahel (Larwanou et al, 2006), a été expérimentée en zone tropicale humide avec des villageois de République Démocratique du Congo (RDC) par le projet européen Makala, depuis 2010. La RNA pourrait constituer une solution viable pour lutter contre la savanisation des dernières forêts galeries du plateau Batéké, tout en pérennisant les productions agricoles et de charbon. [less ▲]

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See detailHR managers views and practices in SME's regarding return to work of sick listed employees
Mairiaux, Philippe ULg; Lequeux, Sarah; Lambreghts, C et al

Poster (2012, October 24)

Background Little is known about the return to work (RTW) policies implemented in small and medium size enterprises (SME) which have limited resources to define and apply such policies. This study aimed ... [more ▼]

Background Little is known about the return to work (RTW) policies implemented in small and medium size enterprises (SME) which have limited resources to define and apply such policies. This study aimed at assessing the existing return to work practices in SME’s and exploring their view regarding the help they could receive from their occupational health service. Methods HR managers of 46 SME’s (23 in Flanders, 23 in Wallonia) employing 100 to 200 employees were contacted for an interview. The manager was asked to fill in a written questionnaire and to comment his/her answers in an open discussion with the researcher. The questionnaire involved three parts: 1°) describing existing rules or procedures when returning to work after sick leave, 2) assessing the manager knowledge of the RTW legal regulations within the occupational health care system, 3) assessing the occupational health physician (OP) involvement in RTW and the enterprise expectations for the future. Results Participation to the survey was accepted in 38 SME’s. In slightly more than half of them a sick leave related policy has been defined. While almost all SME’s have a well-defined procedure for the RTW examination by the OP, only 22 (out of 38) have defined procedures for maintaining a contact with the worker during the sick leave period, only 17 are informing their employees about the possibility to meet the OP during that period, and only 6 are systematically organising a worker-supervisor meeting when the worker comes back at work. Knowledge about RTW regulations is rather poor: 10 managers wrongly think that the OP may check the sick leave medical validity; only 14 managers know that the employer must inform the OP about any sick leave longer than 4 weeks; less than half of the managers have a good knowledge of the pre-return to work visit. Various expectations have been put forward as regard the role of the OH service: knowledge transfer about the regulations, coaching of the supervisors, training managers in carrying RTW talks with the worker, suggesting procedures, etc. Conclusions The lack of knowledge about RTW regulations underlines the need for information campaigns focused on the employers. The participating RH managers are awaiting a more proactive role from their OH service. [less ▲]

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See detailAdapting the Sherbrooke model to the Belgian situation
Mortelmans, Katrien; Verjans, M; Mairiaux, Philippe ULg

Poster (2012, October 23)

In Canada and the Netherlands, randomized controlled trials proved that the Sherbrooke model is an effective method to induce sustainable work resumption for patients off work over one month due to back ... [more ▼]

In Canada and the Netherlands, randomized controlled trials proved that the Sherbrooke model is an effective method to induce sustainable work resumption for patients off work over one month due to back problems. The model offered an individually tailored, workplace oriented, step-by-step multidisciplinary return to work strategy. Prior to implementing the model in Belgium, this study aimed at adapting it to the Belgian social security system and labour market needs. A qualitative study was conducted in 2011, with the support of the European Social Fund: discussion groups were organised with sickness absence benefit insurers; employers and workers unions; physicians, ergonomists, psychologists and nurses involved in disability management; and academic experts including the international group of trainees and mentors of the Strategic Training Program in Work Disability Prevention. The meetings aimed at collecting ideas and proposals for model adaptation. For the final model adaptation, the research team relied on a multidisciplinary expert group. To answer company’s requirements a tool was developed making co-workers co-responsible for the worker’s successful work resumption trajectory. To enhance the workers’ own responsibility in the return to work process, a patient return to work diary was designed. To take into account the rising trend in mental health reasons for prolonged sickness absence, the model was opened up to patients irrespectively of the cause of their sickness absence. Belgian employers having limited financial incentives for return to work initiatives when sickness absence periods exceed one month, it was decided to a) implement the model in sectors having difficulties finding suitable personnel due to the labour market scarcity, and b) ask participating companies to commit to fulfil 85% of multidisciplinary work resumption advices and to report in detail on non-followed advices. The Sherbrooke model was mirrored among the Belgian situation and adapted to Belgium’s specific needs. In 2012, the adapted model will be evaluated in a pilot study including five companies employing about 8000 workers in the Antwerp region of Belgium. [less ▲]

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See detailOPTIMIZATION OF HUMAN ES CELLS (hESCs) CRYOPRESERVATION
Connan, Delphine ULg; Ectors, Fabien ULg; Grobet, Luc ULg et al

Poster (2012, October 22)

Detailed reference viewed: 30 (13 ULg)
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See detailCharacterization and transfection experiments of polyplexes targeting HDAC7
Frère, Antoine ULg; Kawalec, Michal; Collard, Laurence ULg et al

Poster (2012, October 22)

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See detailCaractérisation du mouton Koundoum du Niger : description morphobiométrique et détermination d'une formule barymétrique
Hamadou, Issa ULg; Marichatou, Hamani; Moula, Nassim ULg et al

Poster (2012, October 20)

La race Koundoum, un des rares moutons à laine du Sahel, est victime des croisements avec d’autres races plus productives et de la dégradation de son biotope originel, le fleuve Niger. Cette race possède ... [more ▼]

La race Koundoum, un des rares moutons à laine du Sahel, est victime des croisements avec d’autres races plus productives et de la dégradation de son biotope originel, le fleuve Niger. Cette race possède des qualités intéressantes d'adaptation au milieu et de production de laine, qui justifient la mise en œuvre d’un programme de conservation de ce patrimoine génétique original. Dans cette optique, notre étude vise à la caractérisation de la race Koundoum afin d’établir les fondements d’un plan pour sa conservation. Il a ainsi été procédé à la caractérisation morpho-biométrique de moutons de race Koundoum auprès de 26 éleveurs, principalement de la commune de Kourteye. Au total, l’étude a porté sur 147 sujets (45 mâles, 102 femelles), dont 56 de moins d’un an. La couleur de robe noire domine (62,6%), suivie de la couleur blanche (27,9%). Les pendeloques sont présentes chez 40,0% des mâles et 26,5% des femelles. Presque tous les béliers portent des cornes (95,6%) contre 3,9% des brebis. Le périmètre thoracique a été retenu pour l’estimation d’une équation barymétrique en raison de sa forte corrélation avec le poids (R2 = 0,88). Des formules de prédiction du poids ont pu être proposées selon le sexe et l’âge. En dépit du manque de contrôle des croisements par la majorité des éleveurs inclus dans l’étude, l’échantillon a révélé des caractéristiques relativement homogènes. La détermination d’une formule barymétrique pourra venir en soutien d’une sélection incluant un objectif de croissance. [less ▲]

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See detailQuality characteristics of marketed eggs in Basse Kabylie (Algeria)
Moula, Nassim ULg; Philippe, François-Xavier ULg; Ait Kaki, Asma ULg et al

Poster (2012, October 19)

Quality variations of retailed eggs are widely reported. This study aims at assessing the quality of eggs according to the marketing channel in the department of Bejaia (Algeria). In spring and summer ... [more ▼]

Quality variations of retailed eggs are widely reported. This study aims at assessing the quality of eggs according to the marketing channel in the department of Bejaia (Algeria). In spring and summer 2012, a total of 3330 eggs were bought in 30 stores divided into 3 categories: 10 supermarkets (1146 eggs), 10 public markets (1048 eggs), and 10 shops (1136 eggs). The eggs weights differed significantly with the marketing channel with 58.9±0.14, 61.2±0.13 and 62.8±0.13 g for public markets, shops and supermarkets, respectively (p<0.001). Although the shell thickness was similar for all marketing channels, the proportion of damaged eggs was higher in public markets (9.0%), intermediate in shops (7.3%) and lower in supermarkets (5.7%; p<0.05). The yolk/albumen ratio was significantly higher for eggs from supermarkets (48.1%) compared to the other channels (around 47.4%; p<0.05). The freshness of the eggs, measured by the Haugh method, was lower in public markets (74.3 units), intermediate in shops (77.6 units) and higher in supermarkets (79.9 units; p<0.05). The price of eggs, expressed in Algerian Dinar (AD) per kg, was significantly lower in public markets (124 AD/kg) compared to the two other channels (around 131 AD/kg; p<0.05). One can conclude that egg quality in Basse Kabylie differs significantly between marketing channels with higher quality observed in supermarkets. The lower quality of eggs in public markets is associated with lower price. Eggs from shops present an intermediate quality. A one-year study would allow studying both the potential seasonal effect and compare intrinsic variability across marketing channels. [less ▲]

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See detailEncephalitozoonosis in a pet dwarf rabbit.
Caron, Yannick ULg; Abos, Romain ULg

Poster (2012, October 19)

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See detailCritères d’appréciation des reproducteurs en systèmes d'élevage traditionnel du zébu Azawak dans les zones d’Abalak, Filingué et Niamey (Niger)
Siddo, Seyni ULg; Moula, Nassim ULg; Marichatou, Hamani et al

Poster (2012, October 19)

Au Niger, le zébu Azawak fait l’objet d’un programme de sélection au sein de la Station de Toukounous, créée en 1954. Si de substantiels progrès en termes de production individuelle ont été atteints, ce ... [more ▼]

Au Niger, le zébu Azawak fait l’objet d’un programme de sélection au sein de la Station de Toukounous, créée en 1954. Si de substantiels progrès en termes de production individuelle ont été atteints, ce matériel génétique nécessite d’être diffusé à travers le pays. Cette étude caractérise les critères d'appréciation des géniteurs Azawak auprès de 120 éleveurs d’Azawak non-améliorés au sein de trois contextes d’élevage différents: pastoral (Abalak, n=38), agricole (Filingué, n=54) et périurbain (Niamey, n=28). Près de 65,5% des éleveurs interrogés apprécient l’Azawak pour sa production laitière, sa valeur socioculturelle, l’esthétique de la robe, la facilité d'engraissement et sa docilité pour la traction. Les motifs mentionnés par les 34,5% d'éleveurs affirmant ne pas apprécier le zébu Azawak amélioré sont son exigence en compléments alimentaires et en soins vétérinaires et son inadaptation à la transhumance. Les critères majeurs du choix du géniteur sont la longueur de la queue (60,0%), la grosseur du fourreau (58,2%), la performance laitière des descendants (55,0%), ainsi que leur bonne conformation (48,3%) et leur docilité (46,7%). La réforme des mâles par l’éleveur est dictée par le comportement d'errance (67,7%), l'indocilité (51,9%) et la mauvaise conformation des descendants (42,6%). Les déterminants de l’appréciation des reproducteurs par les éleveurs sont le fondement des objectifs et critères de sélection. L’inadéquation entre les deux résulte en une absence de diffusion du matériel amélioré. La compréhension de ces contraintes à la diffusion permettra leur prise en compte au niveau du programme de sélection ou des autres services d’élevage. [less ▲]

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See detailClinical Assessment of alfaxolone in rabbits
Tutunaru, Alexandru-Cosmin ULg; Sonea, Alexandru; Drion, Pierre ULg et al

Poster (2012, October 19)

Detailed reference viewed: 13 (1 ULg)
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See detailEvaluation of the non-genetic factors of the racing performances of Arabian horses in Algeria
Tennah, Safia ULg; Kafidi, Nacerredine; Antoine-Moussiaux, Nicolas ULg et al

Poster (2012, October 19)

Selective breeding of Arabian horses in Algeria is not yet well developped. No accurate estimation of the breeding values of racing horses is carried out untill now. The estimation is based on one or ... [more ▼]

Selective breeding of Arabian horses in Algeria is not yet well developped. No accurate estimation of the breeding values of racing horses is carried out untill now. The estimation is based on one or several measurable traits, linked to race performance. To allow for an accurate estimation, it is important to determine the part of the phenotypic variability that is due to non-genetic factors (environmental). This first study estimates phenotypic correlations between the recorded traits in order to deduce to what extent the different measures bring different information on animal performance. Then it estimates the influence on performance of non-genetic factors such as sex, age, year of performance and the interactions between these various factors. From 1995 to 2007, the data related to flat racing was collected. The General Linear Model was used to identify and quantify the non-genetic factors affecting racing performances. Three traits were used: two earnings traits (the logarithm of annual virtual earnings: LAEV and the logarithm of average annual virtual earnings per start: LAEV/S), and one ranking trait (the ranking transformed and normalised: PERF). Significant high positive correlations was observed between the three traits (p<0.001), which thus give account of similar informative aptitudes. The effects of sex, age, year as well as the interactions between age and sex and between age and year turned out to be significant for the three traits (p<0.05). These results indicate the need to adjust the earnings and ranks traits in order to use them as criteria of selection of racing Arabian horses. [less ▲]

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See detailInvestigation of the effect of exercise on the innate immunity in horses
Frellstedt, Linda ULg; Gosset, Philippe; Desmet, Christophe ULg et al

Poster (2012, October 19)

Detailed reference viewed: 34 (14 ULg)
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See detailEffect of body weight losson pulmonary function assessed by 6-minute walk test and arterial blood gases in obese dogs
Manens, Jeff; Ricci, Rebecca; Damoiseaux et al

Poster (2012, October 19)

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See detailValidation of a method for measuring the colour and determining the proportions of myoglobin redox forms on beef
Didimo Imazaki, Pedro Henrique ULg; Salmon, Olivier; Tahiri, Assia ULg et al

Poster (2012, October 19)

The aim of this study was to validate the use of the spectrophotometer Minolta CM-600d for measuring the colour and the proportions of different myoglobin redox forms (oxymyoglobin, deoxymyoglobin, and ... [more ▼]

The aim of this study was to validate the use of the spectrophotometer Minolta CM-600d for measuring the colour and the proportions of different myoglobin redox forms (oxymyoglobin, deoxymyoglobin, and metmyoglobin) on the surface of meat. One vacuum-packaged (VP) striploin was supplied by a Belgian food wholesaler. It was cut in 3 cm thick steaks, repacked under vacuum and stored at −0.5 °C until analyses. The measurement of colour in the C.I.E. L*a*b* space and the determination of oxymyoglobin, deoxymyoglobin, and metmyoglobin were performed on VP and modified atmosphere-packed (70 % O2/30 % CO2 for 24 h) samples (n = 10). Results obtained were compared to two reference methods (colour measurement using a chromameter Minolta CR-400 and spectrophotometric determination of different myoglobin redox forms in aqueous meat extracts) by F-test for precision and t-test for accuracy. Statistic significance level was established at 5 %. The two colour measurement methods presented the same precision, when considering VP samples only, and different accuracies, probably because of the different detectors and observation angles used by both devices. The two methods for determining the different myoglobin forms presented also the same precision but different accuracies, probably due to the fact that oxygenation is favoured during some steps of the reference method (e.g. extraction, filtration). In conclusion, the results for colour measurement obtained by both devices cannot be compared. It is necessary to compare both methods for determining oxymyoglobin, deoxymyoglobin, and metmyoglobin in complete anaerobic conditions in order to eliminate the oxygenation bias. [less ▲]

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See detailCulicoides (Diptera : Ceratopogonidae) : important vectors of cattle diseases. Control assays in Belgium
Smeets, François ULg; Robert, Nancy; Simonon, Grégory et al

Poster (2012, October 19)

Detailed reference viewed: 33 (14 ULg)
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See detailSelection criteria as used by owners-breeders of racehorses in Algeria
Tennah, Safia ULg; Farnir, Frédéric ULg; Kafidi, Nacereddine et al

Poster (2012, October 19)

Due to positive externalities and the need to work on a large scale, animal breeding is usually carried out by breeders associations or states. In Algeria, racehorse breeding is in the hands of ... [more ▼]

Due to positive externalities and the need to work on a large scale, animal breeding is usually carried out by breeders associations or states. In Algeria, racehorse breeding is in the hands of unorganized breeders-owners. Through a survey conducted with 461 owners-breeders between 2009 and 2011, this study investigates their perceptions, objectives and practices regarding selective breeding. Racehorse breeding is a full-time professional activity for a third of interviewees. The holdings are small-sized with 77% owning one or two mares. The regular practice of insemination could categorize breeders according to their professionalization (38.4% professional vs. 61.6% occasional breeders). Seniority in the sector was also used to classify breeders, considering as “young” the breeders under 10 years experience (38.8%) and as “senior” those above 10 years (61.2%). More than professionalization, seniority shows a significant impact on practices and objectives. Thus, seniority influences breed choice (young breeders tend to specialize while senior own both Arabian and Thoroughbreds; p<0.001), age at first foaling (sooner among senior breeders; p<0.01), information sources considered for selecting stallions (senior use more diversified sources; p<0.01), the importance granted to the price of insemination (greater for the young breeders; p<0.001), the importance granted to the ranking compared to earnings (the ranking being more important to young breeders, p<0.001), and the priority given to breeding (young breeders give rather priority to a buy-race-resell activity; p<0.001). Finally, racehorse breeding in Algeria is poorly professionalized, the only financial goal being cost coverage. Despite inappropriate practices, an interest for selection is noticed. [less ▲]

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See detailSemen quality of stress negative Piétrain and Duroc boars in the tropics: the case of Vietnam
Do Duc, Luc ULg; Binh, Dang Vu; Leroy, Pascal ULg et al

Poster (2012, October 19)

This study was carried out in Dong Hiep pig farm, North of Vietnam in order to evaluate the semen quality of stress negative Piétrain and Duroc boars. A total of 722 ejaculates from 13 homozygous (CC), 7 ... [more ▼]

This study was carried out in Dong Hiep pig farm, North of Vietnam in order to evaluate the semen quality of stress negative Piétrain and Duroc boars. A total of 722 ejaculates from 13 homozygous (CC), 7 heterozygous (CT) stress negative Piétrain and 10 Duroc CC boars was collected between 2008 and 2012. The sperm quality was assessed on each ejaculate using ejaculate volume (VOL), spermatozoa motility (MO), sperm concentration (CO) and total number of spermatozoids (NT). Genetic type of boars, season, year and (season x year) as well as (genetic type x year) interactions were included in the model as fixed factors. The results show that the semen quality was influenced by all studied effects (p<0.05) except VOL for season (p=0.45) and season x year (p=0.55), and CO for genetic type (p=0.93). VOL and NT (291.74ml and 103.37×109spz) of Piétrain CC were higher than those (241.40ml and 84.58×109spz) of Piétrain CT and (228.05ml and 77.15×109spz) of Duroc (p<0.001) although the values of the 3 genetic groups are in the range of normal semen. MO, CO and NT tend to be higher in cold than in hot season (p<0.001). These results suggest that semen from Piétrain and Duroc boars could be used in tropical climatic conditions (particularly Piétrain CC) and that the semen quality could be improved through reduction of heat stress. [less ▲]

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See detailCCL2 as a serum biomarker of idiopathic pulmonary fibrosis in dogs
Krafft, Emilie ULg; Roels, Elodie ULg; Heikkilä, H.P. et al

Poster (2012, October 19)

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See detailCardiac ultrasound in canine emergencies with a systemic inflammatory response syndrome
Gommeren, Kris ULg; Desmas, Isabelle; Garcia, Alexandra et al

Poster (2012, October 19)

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See detailCaractérisation des performances d’élevage et de production de deux variétés de la race Ardennaise
Moula, Nassim ULg; Michaux, Charles ULg; Philippe, François-Xavier ULg et al

Poster (2012, October 19)

La poule Ardennaise est une race emblématique de la biodiversité avicole belge. Dans un contexte mondial favorable à la conservation des races locales d’animaux domestiques, cette étude est consacrée à la ... [more ▼]

La poule Ardennaise est une race emblématique de la biodiversité avicole belge. Dans un contexte mondial favorable à la conservation des races locales d’animaux domestiques, cette étude est consacrée à la comparaison de deux variétés de la race Ardennaise (Noire dorée et Noire Argentée). La comparaison est réalisée par le biais de trois études: (1) caractérisation morpho-biométrique des deux variétés; (2) caractérisation de la croissance et de la qualité de la carcasse et de la viande; (3) suivi du taux de ponte pendant 52 semaines et l’étude de la qualité des œufs à 30, 45, 60 et 75 semaines d’âge. Des différences significatives ont été enregistrées au niveau des poids corporels, du grand diamètre du tarse, de la longueur du tarse et de la taille de la crête des deux variétés. La variété de la poule Ardennaise n’influence pas significativement les caractères quantitatifs et qualitatifs de production de viande (rendement, poids après abattage, pH, couleur de la viande). Elle influence cependant significativement le poids de l’œuf entier, le poids du blanc, le pourcentage du blanc, le pourcentage du jaune, le rapport jaune/blanc et le pH du blanc (p<0,05). Pour plusieurs caractères morphologiques, d’engraissement et de ponte, les deux variétés ne présentent aucune différence significative. Il serait intéressant de compléter cette étude par une analyse moléculaire permettant de préciser le degré de similitude génétique entre les deux variétés et éventuellement avec les autres variétés de la race. [less ▲]

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See detailRessources génétiques animales en Kabylie (Algérie)
Moula, Nassim ULg; Iguer-Ouada, Mokrane; Touazi, Leghel et al

Poster (2012, October 19)

La Kabylie est une région côtière montagneuse du nord-est algérien. Ce poster présente les principales espèces et races élevées dans cette région. Les ovins sont représentés par la race Tazegzawt ... [more ▼]

La Kabylie est une région côtière montagneuse du nord-est algérien. Ce poster présente les principales espèces et races élevées dans cette région. Les ovins sont représentés par la race Tazegzawt, répertoriée récemment. Elle est reconnaissable à ses tâches noires à reflets bleuâtres, son nom kabyle signifiant bleu. Son poids peut dépasser 30 kg à 6 mois. La population locale de chèvres n’a pas fait l’objet de descriptions. Toutefois, la chèvre locale est adaptée aux massifs montagneux. Elle est de petite taille avec un poids vif d’environ 25 kg. Elle présente de longs poils et différentes couleurs de robe. La race bovine locale est issue de l’adaptation de la race brune de l’Atlas à un climat tempéré. Elle possède un petit gabarit (de 250 à 300 kg) et une robe grise ou allant du fauve brunâtre au rouge-brun. Par l’industrialisation de la production de volailles, les poules locales ont subi une érosion génétique sévère. Elles présentent ainsi une grande variabilité phénotypique et sont de taille moyenne (autour de 1,5kg). Le lapin local connaît une situation similaire et présente un poids de 2 à 3 kg. Il existe une tradition apicole importante en Kabylie. Les types d’abeilles exploitées ne sont toutefois pas encore caractérisés. Toutes les races citées ici sont menacées par le croisement ou l’abandon. Leur caractérisation génétique et zootechnique est nécessaire pour l’amélioration des systèmes de production familiaux, plus particulièrement en régions montagneuses, et la conservation de la biodiversité. [less ▲]

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See detailVers un système de prédiction du rendement en temps réel
Dumont, Benjamin ULg; Ferrandis Vallterra, Salvador ULg; Leemans, Vincent ULg et al

Poster (2012, October 16)

L'objectif de cette recherche est le développement d'un outil capable de prédire les rendements d'une culture de blé en temps réel, au fur et à mesure que la saison avance. Pour atteindre cet objectif ... [more ▼]

L'objectif de cette recherche est le développement d'un outil capable de prédire les rendements d'une culture de blé en temps réel, au fur et à mesure que la saison avance. Pour atteindre cet objectif, nous avons développé une méthodologie qui repose sur l'adjonction des éléments suivants : (i) le modèle de culture STICS (INRA, France), (ii) un réseau de capteurs sans fil pour le monitoring des variables agro-environnementales (éKo system, The Crossbow technologies, USA) et (iii) une base de données météorologiques. [less ▲]

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See detailSingle-cell activity and local field potentials in the ventral tegmental area of awake, freely moving rats
Stanislav, Koulchitsky; Dethier, Julie ULg; Bullinger, Eric et al

Poster (2012, October 16)

The ventral tegmental area (VTA) is known to be involved in reward analysis and detection of salience of events. It contains dopaminergic (DA), GABAergic and perhaps other neurons. Much is known about the ... [more ▼]

The ventral tegmental area (VTA) is known to be involved in reward analysis and detection of salience of events. It contains dopaminergic (DA), GABAergic and perhaps other neurons. Much is known about the activity of DA neurons in anaesthetized animals and brain slices. However, there is a clear lack of data on their dynamic regulation in awake animals, although their reactivity to drugs of abuse, for example, is dramatically different in these different conditions (Koulchitsky et al., 2012). Moreover, little is known about the network activity of the VTA. <br /><br />Using a telemetric recording system and 8-microelectrode-arrays, spanning most of the extent of the VTA, we observed both single cell activity and local field potentials (LFPs), as recently described by others (Fujisawa and Buzsáki, 2011). Neurons were considered as being DA when their firing rate was decreased by more than 50% by an i.p. injection of 100 µg/kg of quinpirole. <br /><br />The firing rate of individual DA neurons was very variable over time, as was the spectrum of LFPs. In many cases, sharp changes in the firing rate/bursting of some DA neurons correlated with an increase in the amplitude of the theta band (~5-8 Hz). They were also coherent to the locomotor activity of the animals. However, the extent to which the firing rate of individual neurons within a rat correlated with the increased theta rhythm was very variable, suggesting that the functional connectivity of different DA neurons at a given moment is quite heterogenous. We are currently attempting to use our recordings to infer a “functional topography” of the VTA. [less ▲]

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See detailDetermination of the molecular players of adaptation to anti-angiogenic therapy in breast cancer by quantitative proteomic and high molecular MALDI Imaging.
Cimino, Jonathan ULg; Sounni, Nor Eddine ULg; Calligaris, David ULg et al

Poster (2012, October 13)

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive ... [more ▼]

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive pre-clinical researches on angiogenesis and finally the approval of VEGF-neutralizing antibodies (bevacizumab) and VEGF receptor tyrosine kinase inhibitors (RTKs:Sunitinib). The Sunitinib has been used clinically in patients with breast cancer refractory to other therapeutic agents. Unfortunately, like the cytotoxic therapies, these drugs do not produce lasting effects and resistance to treatment appeared clinically. Questions have emerged about the failure of anti-angiogenic therapy in clinic and the limitations of predictive preclinical models, and also about the molecular assessment of all stages of tumor adaptation and me<x>tastatic disease. To this end, we applied quantitative proteomics and imaging mass spectrometry tools to visualize and study the profiles of proteins and small molecules associated with tumor treated or not with Sunitinib using a novel preclinical model of breast carcinoma cells. In this project, we first developed a reproducible model of resistance to Sunitinib of human triple negative breast cancer MDA-MB-231 cells expressing luciferase gene. Cells were subcutaneously injected into mice RAG1-/- and divided into four experimental groups including, control mice treated with vehicle or Sunitinib for 30 days and sacrificed 1 days after treatment withdrawal or when tumor reached a volume of 300 mm3. In the second step. Tumors were analyzed using a nanoAcquity UPLC Synapt TM HDMS TM G1 (Waters, Manchester,UK) and Mass Spectrometry Imaging. For quantitative proteomic analyses of tumors, a bioinformatics analysis was used with the Protein lynx global server 2.2.5 software. Imaging mass spectrometry was performed on tissue sections of tumors and organs subsequently colonized by me<x>tastases. Matrix sublimation was used to coat tumor sections (14 µm-tick) with 1.5 Diaminonaphthalene for lipids analysis and Sinapinic acid for entire proteins analysis. Ion cartographies were recorded with a Solarix 9.4T FTMS instrument for lipids and with an Ultraflex II TOF-TOF instrument for entire proteins (Bruker Daltonics, Germany) with a spatial resolution of 100 µm. Global protemic revealed different protein profiles between tumor treated or not with Sunitinib. The Mass Spectrometry Imaging detected differences in intensity and location of some proteins and lipids are also associated with some histological features including inflammatory, necrotic and angiogenic areas. Bioinformatics analysis will be applied to ensure the integration of all data in order to provide the basis for identifying molecular pathways activated during the acquisition of refractoriness to drug treatments. [less ▲]

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See detailSynthesis and physico-chemical studies of glycine betaine derivatives
Laurent, Pascal ULg; NSimba Zakanda, Francis; Mvumbi Lelo, Georges et al

Poster (2012, October 11)

Design of new surfactants is of considerable interest in order to obtain materials with specific physico-chemical properties for targeted applications. Among them, quaternary ammonium surfactants are ... [more ▼]

Design of new surfactants is of considerable interest in order to obtain materials with specific physico-chemical properties for targeted applications. Among them, quaternary ammonium surfactants are widely employed in pharmaceutical and cosmetic industries. Unfortunately, as a consequence of their widespread use and strong resistance to biodegradation, those chemical surfactants may persist in wastewater treatment systems at relatively high concentrations and can cause a disturbance of the ecological equilibrium. In this context, glycine betaine based surfactants are of increasing interest today thanks to their higher biodegradability and low environmental impact. For example, alkylbetaines and alkylamidobetaines are produced on the industrial scale and are used as amphoteric surfactants less irritating compared to sodium dodecylsulfate (SDS) in cosmetics. In this study, new surface-active agents based on glycine betaine and issued from the green chemistry are synthesized. Very simple synthetic methodologies led to different betaine derivatives (esters, thioesters, amides). Their physico-chemical properties are investigated in order to give insights about the key parameters to take into account for the formulation of betaine derived compound preparations for cosmetic or pharmaceutical purposes. [less ▲]

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See detailDetection and Quantification of Efficiency and Quality of Gait Impairment in Multiple Sclerosis through Foot Path Analysis
PHAN BA, Remy ULg; Pierard, Sébastien ULg; MOONEN, Gustave ULg et al

Poster (2012, October 11)

Introduction: Walking speed is generally considered as the best outcome measure in trials for people with multiple sclerosis (pMS). We recently designed a device based on range laser scanner capable to ... [more ▼]

Introduction: Walking speed is generally considered as the best outcome measure in trials for people with multiple sclerosis (pMS). We recently designed a device based on range laser scanner capable to track feet paths of walking subjects. Our purpose was to explore gait descriptors of pMS and compare them with those of healthy volunteers (HV). Methods: Fourty-four pMS (considered as moderatly or highly disabled according to a cut-off EDSS value of 3.0) and 28 HV performed 4 walking tasks along 2 trajectories in 3 walking modes. Twenty-six gait descriptors crudely dichotomized in « efficiency» and « quality » of gait were compared in the 2 populations using unpaired t-tests. Results: (i) apart from an older age in pMS, the two populations were comparable, (ii) efficiency of gait descriptors including walking speed distinguished HV from pMS, and pMS with moderate from pMS with high disability, (iii) quality of gait descriptors were also significantly altered in pMS, including in walking tasks where their walking speed was comparable to that of HV. Conclusions: RLS technology can distinguish pMS from HV according to (i) more efficiency of gait descriptors than the sole walking speed and (ii) quality of gait descriptors, including in subjects with a « normal » walking speed. [less ▲]

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See detailEvaluation de la recharge des eaux souterraines en Région wallonne : apport de la modélisation EPICgrid et applications
Sohier, Catherine ULg; Grandry, Maud; Gailliez, Sébastien et al

Poster (2012, October 10)

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See detailLe logiciel PegOpera : un outil pour la gestion intégrée des eaux de surface
Grard, Aline ULg; Everbecq, Etienne ULg; MAGERMANS, POL ULg et al

Poster (2012, October 10)

Le logiciel PegOpera inclut la version 5 du modèle Pegase (Planification Et Gestion de l’ASsainissement des Eaux) et une nouvelle interface graphique. PegOpera est un outil personnalisé utilisé par les ... [more ▼]

Le logiciel PegOpera inclut la version 5 du modèle Pegase (Planification Et Gestion de l’ASsainissement des Eaux) et une nouvelle interface graphique. PegOpera est un outil personnalisé utilisé par les gestionnaires de l’eau et opérationnel dans les champs d’applications de la Gestion Intégrée des Ressources en Eau (assainissement, DCE, NQE, …). [less ▲]

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See detailImplementation of structured metal packing for the design of biofilm reactor : analysis by high energy X-ray tomography and application to the production of lipopeptides by Bacillus subtilis
Zune, Quentin ULg; Soyeurt, Delphine; Ongena, Marc ULg et al

Poster (2012, October 08)

1. Whereas multi-species biofilm reactors are commonly used for treatments of water and gas effluents, new strategies are arising for the development of mono-species biofilm reactors in order to produce ... [more ▼]

1. Whereas multi-species biofilm reactors are commonly used for treatments of water and gas effluents, new strategies are arising for the development of mono-species biofilm reactors in order to produce high added value molecules. Thus, it is required to design new bioreactors able to promote the growth of the biomass on the form of a biofilm and to identify the key physico-chemical parameters involved in order to optimize the bioprocess. 2. Aim of this study was to investigate a pilot-scale biofilm reactor comprising a metal structured packing promoting growth of Bacillus subtilis as a biofilm for the production of lipopeptides, high added value compounds with high surface active properties. 3. In this work, the mechanical stirring system of a 20L stirred tank bioreactor has been removed and replaced by a metal structured packing positioned in the headspace of the vessel above a liquid phase. The culture medium is continuously recirculated on the packing thanks to a peristaltic pump and air supply is performed just above the liquid phase under the packing. High energy X-ray tomography was used to estimate non-invasively the biofilm distribution inside the packing and permitted to define parameters that affect scale-up. Performances of the biofilm reactor were compared with a submerged culture in a stirred tank reactor in terms of lipopeptides production. 4. After 72 hours of fermentation, 94 % of the total biomass adheres onto the metal packing on the form of a biofilm. The colonization of this latter has been visualized non-invasively by X-ray tomography directly inside the packing and shows a conical repartition of the biofilm mass (about 25% of the total volume of the packing) as well as the presence of clogging. However, unlike the submerged culture, no foam formation appeared during fermentation and surfactin yield reaches 345,4 ± 32,8 mg / L for the biofilm reactor against 277,3 ± 34,4 mg / L in the stirred tank reactor. 5. In conclusion, this experimental setting leads to a major technological progress avoiding foam formation and increasing surfactin production. Nevertheless, significant improvements are required at the level of the biofilm distribution in thin layers inside the packing in order to increase mass transfer and lipopeptides recoveries. Further investigations will be devoted to the optimization of the physico-chemical parameters involved in biofilm distribution. [less ▲]

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See detailLaboratory and field preliminary tests of Metarhizium anisopliae formulated with neem oil (Suneem) against Anopheles gambiae sl adult emergence
Seye, Fawrou; NDIONE, Raymond; Touré, Mamour et al

Poster (2012, October 08)

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See detailA computationally efficient algorithm for the provision of a day-ahead modulation service by a load aggregator
Mathieu, Sébastien ULg; Karangelos, Efthymios ULg; Louveaux, Quentin ULg et al

Poster (2012, October 08)

We study a decision making problem faced by an aggregator willing to offer a load modulation service to a Transmission System Operator (TSO). In particular, we concentrate on a day-ahead service ... [more ▼]

We study a decision making problem faced by an aggregator willing to offer a load modulation service to a Transmission System Operator (TSO). In particular, we concentrate on a day-ahead service consisting of a load modulation option, which can be called by the TSO once per day. The option specifies the maximum amplitude of a potential modification on the demand of the loads within a certain time interval. We consider the specific case where the loads can be modeled by a generic tank model whose inflow depends on the power consumed by the load and outflow is assumed to be known the day before for every market period. The level of the reservoir at the beginning of the market day is also assumed to be known. We show that, under these assumptions, the problem of maximizing the amplitude of the load modulation service can be formulated as a mixed integer linear programming problem (MILP). In order to solve this problem in a computationally efficient manner we introduce a novel heuristic-method. We test this method on a set of problems and demonstrate that our approach is orders of magnitude faster than CPLEX - a state-of-the-art software for solving MILP problems - without considerably compromising the solution accuracy. [less ▲]

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See detailDiversity and breeding sites of mosquitoes (Diptera, Culicidae) potentially vectors of arboviruses in Belgian equestrian farms
Boukraa, Slimane ULg; De la Grandière de Noronha Cotta, Maria Ana ULg; Bawin, Thomas ULg et al

Poster (2012, October 08)

This study aims to determine the potential importance of the livestock farms, especially equestrian, to welcome and favor the proliferation of certain species of mosquito responsible for transmission of ... [more ▼]

This study aims to determine the potential importance of the livestock farms, especially equestrian, to welcome and favor the proliferation of certain species of mosquito responsible for transmission of arboviruses. The study of biodiversity of Culicidae in the horse farms in Belgium is carried out on species sampled at 64 biotopes in six stations study. Five surveys were realized during 2011 (June, July, August and October) and one in 2012 (June). The morphotaxonomic and molecular study of mosquitoes collected showed the presence of ten species: Culisata annulata Schrank, 1776; Anopheles claviger s.s. Meigen, 1804; An. maculipennis s.s. Meigen, 1818; An. messae Falleroni, 1926; Culex pipiens molestus Forskal, 1775; Cx. pipiens pipiens Linné, 1758; Cx. torrentium Martini, 1925; Cx. hortensis hortensis Ficalbi, 1889; Cx. territans Walker, 1856 and Coquillettidia richardii Ficalbi, 1889. Among the 24893 individuals examined in 2011, Cx. pipiens sl and Cx. torrentium represent 68.00% and 22.38% respectively of total harvest. These last species with Cs. annulata, are dominants and ubiquitous in all the horse farms visited. The species of the genus Anopheles have strong ecological requirements and are therefore associated with some special habitats; other species however have a strong ability to adapt and therefore attend a wide variety of biotopes (Cx. pipiens, Cx. torrentium and Cs. annulata). At the horse farms, water troughs and ponds are the most favorable habitats for larval development of Culicidae. The species potentially vectors of arboviruses that can cause problems in epidemiological equestrian farms are Cx. pipiens sl, Cx. torrentium and Cs. annulata. [less ▲]

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See detailA unicellular mechanism to switch a network behavior from tonic activity to synchronous oscillations
Dethier, Julie ULg; Drion, Guillaume; Franci, Alessio et al

Poster (2012, October 08)

Parkinson’s disease (PD) is a neurodegenerative disorder affecting the basal ganglia (BG), a set of small subcortical nervous system nuclei. The hallmark of the disease is a dopaminergic denervation of ... [more ▼]

Parkinson’s disease (PD) is a neurodegenerative disorder affecting the basal ganglia (BG), a set of small subcortical nervous system nuclei. The hallmark of the disease is a dopaminergic denervation of the input stage of the BG, altering information patterns along movement-related ganglia-mediated pathways in the brain, inducing therefore movement disorders such as tremor at rest, bradykinesia, akinesia, and rigidity. It is still unclear how dopamine depletion causes those motor symptoms. Experimental studies have shown that abnormally synchronized oscillatory activities- rhythmic bursting activity at the neurocellular level and beta frequency band oscillations at the network level-emerge in PD at multiple levels of the BG-cortical loops and are correlated with motor symptoms. We propose a computational model of the BG using a novel unicellular mechanism to explain the induction of bursting activity and beta band oscillations in the network. We show how a single change in the dopaminergic level at the input stage of the BG can switch the model from its physiological state to the pathological state. This computational model also proposes a simple mechanism for high-frequency deep brain stimulations. [less ▲]

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See detailImplementation of structured metal packing for the design of biofilm reactor : analysis by high energy X-ray tomography and application to the production of lipopeptides by Bacillus subtilis
Zune, Quentin; Soyeurt, Delphine; Ongena, Marc ULg et al

Poster (2012, October 08)

1. Whereas multi-species biofilm reactors are commonly used for treatments of water and gas effluents, new strategies are arising for the development of mono-species biofilm reactors in order to produce ... [more ▼]

1. Whereas multi-species biofilm reactors are commonly used for treatments of water and gas effluents, new strategies are arising for the development of mono-species biofilm reactors in order to produce high added value molecules. Thus, it is required to design new bioreactors able to promote the growth of the biomass on the form of a biofilm and to identify the key physico-chemical parameters involved in order to optimize the bioprocess. 2. Aim of this study was to investigate a pilot-scale biofilm reactor comprising a metal structured packing promoting growth of Bacillus subtilis as a biofilm for the production of lipopeptides, high added value compounds with high surface active properties. 3. In this work, the mechanical stirring system of a 20L stirred tank bioreactor has been removed and replaced by a metal structured packing positioned in the headspace of the vessel above a liquid phase. The culture medium is continuously recirculated on the packing thanks to a peristaltic pump and air supply is performed just above the liquid phase under the packing. High energy X-ray tomography was used to estimate non-invasively the biofilm distribution inside the packing and permitted to define parameters that affect scale-up. Performances of the biofilm reactor were compared with a submerged culture in a stirred tank reactor in terms of lipopeptides production. 4. After 72 hours of fermentation, 94 % of the total biomass adheres onto the metal packing on the form of a biofilm. The colonization of this latter has been visualized non-invasively by X-ray tomography directly inside the packing and shows a conical repartition of the biofilm mass (about 25% of the total volume of the packing) as well as the presence of clogging. However, unlike the submerged culture, no foam formation appeared during fermentation and surfactin yield reaches 345,4 ± 32,8 mg / L for the biofilm reactor against 277,3 ± 34,4 mg / L in the stirred tank reactor. 5. In conclusion, this experimental setting leads to a major technological progress avoiding foam formation and increasing surfactin production. Nevertheless, significant improvements are required at the level of the biofilm distribution in thin layers inside the packing in order to increase mass transfer and lipopeptides recoveries. Further investigations will be devoted to the optimization of the physico-chemical parameters involved in biofilm distribution. [less ▲]

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See detailLaboratory and field preliminary tests of Metarhizium anisopliae formulated with neem oil (Suneem) against Anopheles gambiae sl adult emergence
Seye, Fawrou; Ndione, Raymond; Touré, Mamour et al

Poster (2012, October 08)

Metarhizium anisopliae have shown great potential for the control of malaria vectors. However, their ability to control aquatic stages of anopheline vectors with conidial formulation is need. In ... [more ▼]

Metarhizium anisopliae have shown great potential for the control of malaria vectors. However, their ability to control aquatic stages of anopheline vectors with conidial formulation is need. In laboratory condition (25°C and 76%RH), we formulated M. anisopliae with emulsifian neem oil (Suneem 1%) before application on An. gambiae larvae at 4, 6, 8, 10 and 12 % (v/v) to determine the LD90. We applied in semi-field environment, the LD90 of the formulation into artificial vats on the Anopheles gambiae sl larvae collected from many breeding sites at dry and rain seasons. In laboratory condition, the LD 50 was 4.4 x 10^6 spores/ml and the LD90 was not obtained 24 after exposure. The probite line equation was Y=1.61 x – 0.55 and R²= 0.9793. The LD 50 was 3.1 x 10^6 and the LD90 was 5.3 X 10^6 spores/ml 48 h after exposure. The probite line equation was then Y= 1.69 x + 1.79 and R²= 0.9757. Microscope magnifying revealed also the fungal attack via cuticle and mycelia germination one dead larvae and pupae. In semi-field environment, treatment revealed that, at 5.3 x 10^6 spores/ml, the formulation has a great emergence inhibition of mosquito adult formation. No significant difference was observed between dry and rain season application of M. anisopliae on the larvae. Therefore, a combination of M. anisopliae with Suneem may provide a more sustainable management strategy for malaria vectors control at the larval stages. [less ▲]

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See detailSmall satellite activities at the University of Liège
Salvador, Lucas ULg; Rochus, Pierre ULg

Poster (2012, October 01)

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See detailEvidence for a partial epithelial-mesenchymal transition in rat auditory organ development
Johnen, Nicolas ULg; Francart, Marie-Emilie; Thelen, Nicolas ULg et al

Poster (2012, October 01)

An epithelial-mesenchymal transition is a biological process that allows a polarized epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype. During ... [more ▼]

An epithelial-mesenchymal transition is a biological process that allows a polarized epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype. During this process, epithelial cells loosen cell-cell adhesion, module their polarity and rearrange their cytoskeleton: intermediate filaments typically switch from cytokeratin to vimentin. They also enhance their motility capacity. The epithelial-mesenchymal transition plays key roles in the formation of the body plan and in the differentiation of multiple tissues and organs but it is also involved in tissue repair, tissue homeostasis, fibrosis, and carcinoma progression. Until now, epithelial-mesenchymal transition has been rarely mentioned in the inner ear organogenesis. In chick, epithelial-mesenchymal transition has been reported as a possible mechanism of semicircular canal morphogenesis. More recently, an in vitro study has also indicated that sensory epithelial cells from mouse utricle can undergo an epithelial-mesenchymal transition to become cells expressing features of prosensory cells. By contrast, epithelial-mesenchymal transition has never been observed during auditory organ morphogenesis. The auditory organ, the organ of Corti, is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. Theses cells are supported by supporting cells. Based on their morphology and physiology, at least four types of supporting cells can be identified in the organ of Corti: inner and outer pillar cells, phalangeal cell and Deiter’s cells. The inner pillar cells and outer pillar cells combine to form the tunnel of Corti, a fluid filled triangular space that separates the single row of inner hair cells from the first row of outer hair cells. The Nuel spaces are another interval in the organ of Corti that is situated between the outer pillar cells and the different rows of outer hair cells and Deiters cells. To determine whether an epithelial-mesenchymal transition may play a role in the morphogenesis of the auditory organ, we studied the spatial localization of several epithelial-mesenchymal transition markers, the cell-cell adhesion molecules and intermediate filament cytoskeletal proteins, in epithelium of the dorsal cochlea during development of the rat organ of Corti from 18th embryonic day until 25th postnatal day. We examined by confocal microscopy immunolabelings on cryosections of whole cochleae with antibodies anti-cytokeratins as well as with antibodies anti-vimentin, anti-E-cadherin and anti-beta-catenin.Our results showed a partial loss of E-cadherin and beta-catenin between supporting cells at P8 and P12, respectively, and a temporary appearance of vimentin in pillar cells and Deiters between P8 and P10. Our results show a local loss of adhesion between supporting cells of the OC from P8, an increase expression of cytokeratins in supporting cells around P10 and a temporary appearance of vimentin in supporting cells at P8-10. These observations suggest that a partial epithelial-mesenchymal transition might be involved in the remodeling of the Corti organ during the postnatal stages of development in rat. [less ▲]

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