Microorganisms in Karsts: a case study in St Anne cave, BelgiumCarnol, Monique ; Willems, Luc ; Malchair, Sandrine ![]() Poster (2011, September 30) Despite the importance of microorganisms as geochemical agents over geological times, their extended metabolic diversity and their essential role in element cycles (i.e. mineral dissolution, precipitation ... [more ▼] Despite the importance of microorganisms as geochemical agents over geological times, their extended metabolic diversity and their essential role in element cycles (i.e. mineral dissolution, precipitation, oxido-reduction processes), microbial community composition and processes as well as their ecological role in karst environments are poorly known. While little was published on cave-dwelling microorganisms until the early 1990s, it is now recognized that microorganisms may mediate many important mineral transformations, originally considered to be inorganic in nature. Indeed, recent evidence (Northup & Lavoie, 2001) proved the implication of microorganisms in karstification through precipitation and dissolution processes, resulting in the deposition of carbonate speleothems, silicates, iron or manganese oxides, sulphur compounds and nitrates and in the breakdown of limestone walls. In this poster, we review some potential processes and signs of microbial activity in caves. We present results of a study on the microbial diversity in the ‘St Anne’ cave, Belgium. We focused on ammonia-oxidizing bacteria (AOB), which are responsible for the first, acidifying step of the nitrification process. Chemical composition of the water, numbers of cultivable bacteria (free and particle-associated bacteria) and the diversity of AOB were studied in waters and sediments of the ‘Chawresse’ (underground river in St Anne), on the cave wall and in the soil aboveground. The use of molecular techniques, based on direct ADN extractions, provide more detailed information on the microbial diversity of an environment, as culture-based techniques retrieve only about 1% of bacterial species present in the environment. Bacterial counts showed that most cultivable bacteria were associated with suspended particles and that their numbers decreased underground. Molecular analyses revealed the presence of AOB in the karst system. Comparison of aboveground and belowground diversity also indicated the possibility of a specific endokarst AOB community. Further research perspectives will be discussed. <br /> <br /> <br />Northup, D.E. and Lavoie, K.H. 2001. Geomicrobiology of caves: A review. Geomicrobiology Journal, 18(3):199-220. [less ▲] Detailed reference viewed: 49 (10 ULg) L'entrepreneuriat féminin au Maroc: Quelles sont les variables contextuelles qui influencent l'entrepreneuriat?Salman, Noura ; ; Poster (2011, September 30) L’objectif principal de cet article est d’explorer les fondements de l’entrepreneuriat féminin au Maroc et de présenter les modèles existants. Ce qui nous amène à poser les questions suivantes : « Quels ... [more ▼] L’objectif principal de cet article est d’explorer les fondements de l’entrepreneuriat féminin au Maroc et de présenter les modèles existants. Ce qui nous amène à poser les questions suivantes : « Quels sont les variables contextuelles et socio-économiques qui influencent l’entreprenariat au Maroc ? » et « qui sont ses femmes qui ont choisi d’entreprendre au féminin, en bravant courageusement les différents obstacles socio-culturelles qui caractérisent la société marocaine? ». Présenté ainsi, notre recherche entend dresser un état de l’art des différentes variables pouvant expliquer les composantes de l’entrepreneuriat féminin au Maroc et de conceptualiser ses mécanismes opérationnels afin de proposer des pistes de réflexions adaptées au contexte marocain et applicables par les femmes entrepreneures. [less ▲] Detailed reference viewed: 11 (0 ULg) Traçabilité du bois et place des Forêts Communautaires camerounaises au sein du processus FLEGT; Doucet, Jean-Louis ; et alPoster (2011, September 29) Detailed reference viewed: 93 (18 ULg) nanostructured carbon materials by surfactant-templating of resorcinol-formaldehyde gels: towards a control of porosity for energy storage applicationsLéonard, Alexandre ; Pirard, Jean-Paul ; Job, Nathalie ![]() Poster (2011, September 26) Detailed reference viewed: 14 (5 ULg) Evidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV)Rodriguez, Sabrina ; ; Poster (2011, September 26) Evidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV) Sabrina M. Rodríguez1*, Karina Trono2, Leandro R. Jones3 1 Molecular ... [more ▼] Evidencia de determinantes de patogenia en las regiones Long Terminal Repeats (LTRs) del genoma del virus de la leucemia bovina (BLV) Sabrina M. Rodríguez1*, Karina Trono2, Leandro R. Jones3 1 Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA), University of Liège (ULg), Belgium. 2 Instituto de Virología, CICVyA, Instituto Nacional de Tecnología Agropecuaria INTA-Castelar, CC 25 (1712), Castelar. 3 División de Biología Molecular, Estación de Fotobiología Playa Unión, CC 15, Rawson, Chubut 9103, Argentina. *E-mail: sabrina.rodriguez@ulg.ac.be El resultado de la infección por el virus de la leucemia bovina (BLV) es diverso. La mayoría de los animales infectados resultan portadores asintomáticos (AL) y cerca del 30% desarrolla una condición benigna denominada linfocitosis persistente (LP). La forma tumoral o linfosarcoma (LS) afecta a un 1-5% de los bovinos infectados. Las bases genético-moleculares del desarrollo de las distintas formas clínicas son desconocidas. Las Repeticiones Terminales Largas (Long Terminal Repeats, LTR) del genoma viral constituyen determinantes genéticos de patogenia en el caso de otros retrovirus. Sin embargo, esta posibilidad no ha sido evaluada para el BLV. Los análisis para probar la correlación entre los caracteres clínicos y genotípicos entre especies deben ser corregidos incluyendo la filogenia del grupo. De otra manera, la historia evolutiva compartida puede comprometer la independencia estadística del análisis. Sobre estas bases, el objetivo de este trabajo consistió en estudiar la influencia de las variaciones genéticas de las regiones regulatorias LTR del BLV en el desarrollo de las diferentes formas clínicas de la infección mediante métodos comparativos filogenéticos, cladísticos y probabilísticos. Con este fin, se secuenció la región 5´LTR de 40 provirus obtenidos a partir de bovinos naturalmente infectados con BLV que presentaron las diferentes formas clínicas (AL, PL, LS). Fueron identificadas siete posiciones polimórficas que mostraron una asociación aparente con la presentación clínica. Una reconstrucción de la filogenia del grupo fue realizada a partir de las secuencias de la región env obtenidas para 28 de los 40 provirus estudiados en este trabajo. En conjunto, los análisis comparativos cladísticos y probabilísticos basados en el alineamiento empírico de las secuencias y la filogenia del grupo sugieren que las posiciones 41 y 56 del 5´LTR podrían estar correlacionadas con la presentación clínica. Los análisis probabilísticos indicaron además una asociación con la patogénesis viral para las posiciones 373, 450, 494 y 505, aunque los soportes estadísticos correspondientes fueron menores en comparación con los soportes obtenidos para las posiciones 41 y 56. Estas observaciones indican que las regiones LTR del BLV podrían constituir determinantes de patogenia. [less ▲] Detailed reference viewed: 8 (2 ULg) Microstructure characterisation of nanocomposite polymeric foams by X-ray microtomographyPlougonven, Erwan ; Marchot, Pierre ; Detrembleur, Christophe et alPoster (2011, September 21) Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon ... [more ▼] Recent advances in microstructured materials have given rise to many new types of composites that exhibit original and interesting physical properties. For example, a nanocomposite made of carbon nanotubes inside a polymer matrix shows exceptional electromagnetic interference shielding effectiveness when foamed. However, the effective properties of such materials strongly depend on the shape and topology of the microstructural cells. An accurate method for investigating the cellular microstructure is X-ray microtomography (XRµT), for it is non-destructive, and it provides 3D geometric information. Although it cannot be used to observe nanofiller dispersion, it has a strong potential for cell structure characterization. In order to reduce the need for trial and error for tailoring these materials, our objective is to characterize, using XRµT, two different types of foaming procedures, namely supercritical CO2 batch foaming and freeze drying. As the resolution is limited compared to cell size, we have developed a novel statistical method based on 3D autocorrelation to determine characteristic length and examine anisotropy. We present results for these two types of foams and show the limitations of this method. [less ▲] Detailed reference viewed: 43 (9 ULg) Biocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on appleKwasiborski, Anthony ; ; Lepoivre, Philippe et alPoster (2011, September 21) The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to ... [more ▼] The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to apply biological control methods. Pichia anomala strain K was previously identified as an efficient biocontrol agent of the main apple pathogens, Botrytis cinerea and Penicillum expansum. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A cDNA-AFLP and gene disruption study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala strain Kh6 (a haploid form of P. anomala strain K displaying the same biocontrol properties). However, these studies suggested also implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing the triple interaction, host/pathogen/antagonist and the proteomic tool allowing to study the ultime expression of the genome without a priori. One 50mm wound per apple were covered by a membrane and inoculated by a P. anomala suspension then by B. cinerea or not. Samples were collected during the exponential and stationary phase to identify the early and later responses to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced by the presence of B. cinerea in exponential and stationary phases were identified by MALDI-ToF. One hundred five and sixty spots of proteins were influenced by the presence of B. cinerea in exponential and stationary phases respectively. In exponential phase, P. anomala Kh6 in absence of B. cinerea uses mainly the glycolysis pathway, whereas in presence of pathogen, it orientates its energetic metabolism to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new orientation, P. anomala Kh6 probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea and prevents the use of nutrients by the pathogen. In stationary phase, no differences in the P. anomala Kh6 energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala Kh6 seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate. [less ▲] Detailed reference viewed: 32 (0 ULg) A robustness study to investigate the performance of parametric and non-parametric tests used in Model-Based Multifactor Dimensionality Reduction Epistasis Detection.Mahachie John, Jestinah ; Gusareva, Elena ; Van Lishout, François et alPoster (2011, September 19) Model-Based Multifactor Dimensionality Reduction (MB-MDR) is data mining technique to identify gene-gene interactions among 1000nds of SNPs in a fast way, without making assumptions about the mode of ... [more ▼] Model-Based Multifactor Dimensionality Reduction (MB-MDR) is data mining technique to identify gene-gene interactions among 1000nds of SNPs in a fast way, without making assumptions about the mode of genetic interactions. By construction, one of the implementations of MB-MDR involves testing one multi-locus genotype cell versus the remaining cells, hereby creating two imbalanced groups for trait distribution comparison. To date, for continuous traits, we have adopted a standard F-test to compare these groups. When normality assumption or homoscedasticity no longer hold, highly inflated results are to be expected. The power and type I error control of MB-MDR under these assumptions has been thoroughly investigated in Mahachie John et al [1]. The aim of this study is to assess, through simulations, the effects of ANOVA model violations on the performance of Model-Based Multifactor Dimensionality Reduction (MB-MDR). We quantify their effect on MB-MDR using default options, but at the same time introduce alternative options with increased performance. The better handling of imbalanced data using robust approaches [2] within a MB-MDR context is exemplified on real data for asthma-related phenotypes. 1. EJHG (2011), Early view 2. David Freedman, Statistical Models: Theory and Practice, Cambridge University Press (2000), ISBN 978-0521671057 [less ▲] Detailed reference viewed: 17 (6 ULg) Comparison Of Different Methods For Detecting Gene-Gene Interactions In Case-Control DataCattaert, Tom ; ; Gusareva, Elena et alPoster (2011, September 19) It is generally believed that epistasis makes an important contribution to the genetic architecture of complex disease, and numerous statistical and bioinformatics methods have been developed to detect it ... [more ▼] It is generally believed that epistasis makes an important contribution to the genetic architecture of complex disease, and numerous statistical and bioinformatics methods have been developed to detect it. We compare several state-of-the-art epistasis detection methods in terms of empirical power, type-I error control, and CPU time. The methods compared include Model-Based Multifactor Dimensionality Reduction (MB-MDR) [1, 2], BOolean Operation-based Screening and Testing (BOOST) [3], EPIBLASTER [4], Random Jungle (RJ) [5], Logistic Regression and PLINK. Our comparative study is based on an extensive simulation study using different two-locus models, exhibiting both main effects and epistasis [3]. In these simulations, 100 SNPs are generated, no LD between them. All genotypes are assumed to be in Hardy-Weinberg equilibrium. Furthermore, 2 disease-associated SNPs are selected, with MAFs set to 0.1, 0.2 and 0.4. The MAFs of the non-disease associated SNPs are uniformly distributed on [0.05, 0.5]. In order to achieve high accuracy in empirical power estimation, all simulation settings involve 1000 replicates. All methods are applied to WTCCC Crohn's Disease data. [1] Calle, M.L. et al. (2008), Tech. Rep. No. 24, Dep. of Systems Biology, Univ. de Vic [2] Cattaert, T. et al. (2011), Ann. Hum. Gen. 75, 78-89 [3] Wan, X. et al. (2010), Am. J. Hum. Gen. 87, 325-340 [4] Kam-Thong, T. et al. (2011), Eur. J. Hum. Gen. 19, 465-471 [5] Schwartz, D.F. et al. (2010), Bioinf. 26, 1752-1758 [less ▲] Detailed reference viewed: 26 (1 ULg) Parameter estimation using slug tests : application to a heat injection and storage experiment; ; et al Poster (2011, September 19) Detailed reference viewed: 26 (10 ULg) Genome-wide epistasis screening for Crohns’ diseaseGusareva, Elena ; Van Steen, Kristel ![]() Poster (2011, September 19) Genome-wide association (GWA) studies of Crohn's disease have identified numerous genes. However, a substantial portion of the heritability of this disease remains unexplained. Some gene variants, not ... [more ▼] Genome-wide association (GWA) studies of Crohn's disease have identified numerous genes. However, a substantial portion of the heritability of this disease remains unexplained. Some gene variants, not detectable via main effects GWA study, may manifest themselves only in interaction with other variants. To search for interacting genes involved in the regulation of Crohn's disease, we performed GWA epistasis screening in a large human cohort (1851 cases/2938 controls) belonging to the Wellcome Trust Case Control Consortium (WTCCC). All subjects were genotyped with the GeneChip 500K Mapping Array Set (Affymetrix chip). SNPs that passed our quality control (359,479 SNPs) were processed in Biofilter (a software package that looks for candidate epistatic genes contributing to disease risk) giving rise to 14,185 SNPs. Subsequent MB-MDR epistasis screening discovered four pairs of interacting SNPs on chromosome 4q35.1 and eight pairs on chromosome 11q23.2. The identified pairs of SNPs were confirmed with synergy-based measures. Notably, despite their mapping to the same genomic regions, the interacting SNPs were not in LD (r^2 < 0.5). Our findings support the idea of close chromosomal localization of two pairs of interacting genes that are involved in development of Crohn's disease. [less ▲] Detailed reference viewed: 28 (5 ULg) Influence of the processing parameters on the physicochemical properties of puff pastry margarinesLefebure, Emilie ; ; Brostaux, Yves et alPoster (2011, September 18) Margarinespresentdifferentphysicalpropertieswithrespecttotheirpurposedapplication.Forexample,aspecialtypeoffatisrequiredforpreparationofpuffpastry.Puffpastrymargarines(PPMs)havetobeveryfirmandplastic ... [more ▼] Margarinespresentdifferentphysicalpropertieswithrespecttotheirpurposedapplication.Forexample,aspecialtypeoffatisrequiredforpreparationofpuffpastry.Puffpastrymargarines(PPMs)havetobeveryfirmandplastic.Therequiredphysicalpropertiesarethusobtainedbyprocessingsuitablyselectedblendsoffatsandoils.Thisstudyfocusesontheinfluenceofprocessingparametersonphysicochemicalpropertiesofpuffpastrymargarine.Forthispurpose,twoselectedfatblendswereconsidered(bothpalm-based):onewaslow-trans(0.5%)andtheotherwastrans-free. [less ▲] Detailed reference viewed: 29 (12 ULg) Influence of the nitrogen source and of the mitochondrial alternative oxidase (AOX) on the response to sulphur deficiency in Chlamydomonas reinhardtiide Marchin, Thomas ; Ghysels, Bart ; Franck, Fabrice ![]() Poster (2011, September 18) Detailed reference viewed: 13 (2 ULg) Establishment of Parathyroid hormone reference range on 10 different assay kits: impact of the recruitment of the populationCAVALIER, Etienne ; Poster (2011, September 18) Detailed reference viewed: 14 (1 ULg) Insertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtiiGodaux, Damien ; ; Cardol, Pierre et alPoster (2011, September 18) The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases ... [more ▼] The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization. [less ▲] Detailed reference viewed: 27 (9 ULg) Automated Measurement of Total 25OH Vitamin D in the IDS iSYS SystemCAVALIER, Etienne ; BEKAERT, Anne-Catherine ; CARLISI, Ignazia et alPoster (2011, September 17) Detailed reference viewed: 20 (3 ULg) Macropinocytosis of human papillomaviruses in natural killer cells via CD16 induces cytotoxic granule exocytosis and cytokine secretionRenoux, Virginie ; Langers, Inge ; et alPoster (2011, September 13) Detailed reference viewed: 20 (5 ULg) Rapidly solidified microstructure of 3D parts fabricated by selective laser melting (SLM) - Examples of stainless steel 316L and titanium Ti-6Al-4VContrepois, Quentin ; Lecomte-Beckers, Jacqueline ![]() Poster (2011, September 12) Laser additive manufacturing process capable of producing fully dense metallic parts direct from 3D CAD know a fast development. Major concerns are made to achieve the best accuracy of the final geometry ... [more ▼] Laser additive manufacturing process capable of producing fully dense metallic parts direct from 3D CAD know a fast development. Major concerns are made to achieve the best accuracy of the final geometry and the reduction of the residual stresses but metallurgical aspects are also essential. The process can be described as a succession of very small welds. A key in the optimization of the mechanical properties is the understanding of the specific solidification mechanisms. Microstructures of two alloys were studied by metallographic examination and EBSD analysis. [less ▲] Detailed reference viewed: 28 (3 ULg) Nanostructured carbon materials by surfactant-templating of resorcinol-formaldehyde gels: towards a control of porosityLéonard, Alexandre ; Pirard, Jean-Paul ; Job, Nathalie ![]() Poster (2011, September 12) Detailed reference viewed: 18 (4 ULg) DOES INCREASING LEVEL OF CONDENSED DISTILLERS SOLUBLES (PROTIWANZE®) SUPPLEMENTATION AFFECT MILK PRODUCTION IN DAIRY CATTLE ?Lessire, Françoise ![]() Poster (2011, September 08) INTRODUCTION The objective of this study was to evaluate whether introducing the condensed distillers solubles Protiwanze® (PW) at different concentrations in dairy cows diet induces changes in milk ... [more ▼] INTRODUCTION The objective of this study was to evaluate whether introducing the condensed distillers solubles Protiwanze® (PW) at different concentrations in dairy cows diet induces changes in milk production. MATERIAL AND METHODS Three herds (72 Holstein and 25 Brown Swiss, DIM = 101 ± 57; daily milk production = 34.90 ± 8.40 L) received successively two RTM differing in PW content for 4 weeks, including a 7-day transition: 0% and 10% on a dry matter basis for Herd 1 and 10% and 15% for Herd 2 and 3. Both concentrations were distributed alternatively and repeated twice, each cow being its own control. Rations were formulated to meet energy and nitrogen requirements. During each testing period, animals were scored (Body Condition, Rumen Fill, Fecal Consistency, Undigested Fraction according to Zaaier (2001)) and their milk production and composition were measured by the Dairy Herd Improvement. RESULTS AND CONCLUSION Increasing the level of PW did not significantly change milk production in any Herd: Values were for Herd 1 at PW0%: 33.08 ± 0.76 L, at 10%: 31.66 ± 0.68 L, for Herd 2 at PW10%: 32.85 ± 0.93 L and at 15%: 32.37 ± 0.89, for Herd 3 at PW10%: 28.71 ± 1.21 L and at 15%: 31.80 ± 1.16. In Herd 1, milk urea decreased from 243.80 ± 4.55 mg/L for PW0% to 218.05 ± 4.03 mg/L for PW10%, (p<0.01), while fat percentage increased from 3.38% ± 0.09 (PW0%) to 3.77% ± 0.08 (PW10%) (p<0.01). Concerning scores, unconclusive changes were observed. Although increasing level of PW in dairy cows diet did not change milk production or composition, it is profitable since PW is 30-40% cheaper than other protein supplements like soya and canola meals. Moreover, it allowed to lower milk cost price by 1,5 eurocent per liter, when used at higher concentration. [less ▲] Detailed reference viewed: 13 (0 ULg) Does feeding dairy cattle with different levels of condensed distillers solubles (Protiwanze®) increase the risk of Sara?Lessire, Françoise ; Rollin, Frédéric ![]() Poster (2011, September 08) INTRODUCTION The objective of this study was to evaluate the effects of different levels of Protiwanze® (PW) supplementation, a highly acid (pH = 3.8 ± 0.8) and fermentescible CDS, on ruminal function of ... [more ▼] INTRODUCTION The objective of this study was to evaluate the effects of different levels of Protiwanze® (PW) supplementation, a highly acid (pH = 3.8 ± 0.8) and fermentescible CDS, on ruminal function of dairy cows. MATERIAL AND METHODS PW supplementation was tested in 5 dairy herds (144 cows, DIM: 96 ± 61, daily milk production: 34.69 ± 8.22 L). In Herd 1, TMR was supplemented with 0% or 10% of PW on a dry matter basis for 4 weeks including a 7-day transition period. Each concentration was distributed twice during 2 periods alternatively with the other one, each cow being its own control. PW concentrations were 10 and 15% in Herd 2, 3 and 4. During every period, milk production was measured by the Dairy Herd Improvement and ruminal fluid sampled by a stomach tube (Ruminator®) on 5 cows. Samples were assessed for pH (portable pH meter), redox potential (Methylene blue test) and protozoa (optical microscopy). RESULTS AND CONCLUSION Ruminal pH values ranged between 5.94 and 7.74. Even when a correction factor of 0.5 was applied to take into account possible saliva contamination, only 8 pH samples pleaded for SARA although protozoa and methylene blue tests were within norms and cows clinically normal. No significant correlation between pH value, milk production and fat content could be demonstrated. Ruminal pH did neither significantly differ between the different levels of PW supplementation. In conclusion, in this study, PW could be used in dairy cows TMR at a level as high as 15% without increasing the risk of SARA. [less ▲] Detailed reference viewed: 13 (2 ULg) Effect of steam explosion pre-treatment on enzymatic saccharification of lignocellulosic materialMeyer, Laurence ; Jacquet, Nicolas ; Vanderghem, Caroline et alPoster (2011, September 08) Taking into account the sharp rise in prices and the depletion of resources of petroleum, an alternative to fossil resources is needed. A probable alternative is the use of lignocellulosic raw material to ... [more ▼] Taking into account the sharp rise in prices and the depletion of resources of petroleum, an alternative to fossil resources is needed. A probable alternative is the use of lignocellulosic raw material to produce biofuels. The “first generation” biofuels are highly controversial because of the use of food plant material. The aim of the “second generation” biofuels is to take lignocellulosic non-food plant material as raw material. Lignocellulosic biomass has a very complex structure made of linkages between lignins, cellulose and hemicelluloses. The saccharification of these lignocellulosic materials requires the fractionation of its constituents. Research has lead to many lignocellulosic biomass fractionation pre-treatments. This study particularly focuses on the steam explosion pretreatment followed by an enzymatic saccharification. Steam explosion is a thermomechanical process which allows the breakdown of the lignocellulosic material structure by the combined action of steam heating, hydrolysis induced by the organic acids formed during the process and shear stress resulting from the pressure rough drop. This treatment leads to modification of the physical parameters such as water retention capacity, cristallinity rate of the cellulosic fraction, hydrolysis of the hemicellulosic fraction and rearrangement in the lignin structure. Such modifications are supposed to make cellulose enzymatic hydrolysis from complex lignocellulosic material easier. In order to verify this hypothesis, different lignocellulosic raw materials have been pre-treated by steam explosion. These materials were sugar beet pulp, corn straw and miscanthus. In order to check the effect of steam explosion pre-treatment on cellulose, a microcrystalline cellulose was also treated. Steam explosion was performed at a vapor pressure of 18 bars and with a retention time of 2 minutes. The steam exploded lignocellulosic materials and the untreated one were submitted to a hydrolysis with a mixture of enzymes composed of cellulases and cellobiase activities during 24 hours. The quantification of glucose in the hydrolysates at different times was performed by HPAEC-PAD. Rate of cellulose converted into glucose were better with steam exploded raw material showing that steam explosion allows improvement of lignocellulosic material for enzymatic saccharification. [less ▲] Detailed reference viewed: 52 (16 ULg) MICRO-H2 – Microbiological production of hydrogen: study of microalgal and bacterial processesCalusinska, Magdalena ; Joris, Bernard ; Wilmotte, Annick et alPoster (2011, September 07) The project MICRO-H2 aims to study and exploit the microbial (bacterial and algal) production of hydrogen (H2). In addition to building a competence centre around the H2 production by microorganisms and ... [more ▼] The project MICRO-H2 aims to study and exploit the microbial (bacterial and algal) production of hydrogen (H2). In addition to building a competence centre around the H2 production by microorganisms and the molecular monitoring of the processes, this project tries to address two main socio-economic issues. First, transport and many economic activities will be based on hydrogen energy in the near future. Secondly, many researches and technology developments deal with renewable resources. Therefore, a new integrated technology for a sustainable development should be promoted. Photofermentation and dark-fermentation are the most promising ways to produce biohydrogen. The main advantage of the first process is the complete conversion of substrate, if any, to hydrogen. However, present H2-production rates by microalgae remain low. Therefore, a better understanding of the microalgal hydrogen metabolism and rate improvements by genetic engineering are needed. On the other hand, dark-fermentation achieves at present far higher H2-production rates, but improvements are expected through monitoring and optimisation of bacterial diversity and activity. The objectives about bacterial H2 production were to increase knowledge, stability potentialities and investigation skills about the consortia of bacteria involved in bioreactors treating wastewater rich in carbohydrates to produce biohydrogen. The project focused mainly on the study of the potentialities of different consortia, with a focus on Clostridium strains. Concerning the microalgal production of H2, the objectives were to increase knowledge on the metabolic interactions that determine H2 evolution at the cellular level and to produce new strains with increased ability for H2 production in the two-stage process. [less ▲] Detailed reference viewed: 31 (7 ULg) Mastitis risk indicators assessed through a germ specific epidemiological model in southern BelgiumTheron, Léonard ; ; et alPoster (2011, September 07) Bovine mastitis is the most prevalent pathology in dairy production; in order to identify herd related risk indicators, our study focused on germ-specific approach to refine known risk indicators between ... [more ▼] Bovine mastitis is the most prevalent pathology in dairy production; in order to identify herd related risk indicators, our study focused on germ-specific approach to refine known risk indicators between environmental or contagious epidemiologic models. We gathered clinical mastitis during 3 months in 21 walloon dairy farms representing 1630 Holstein cows (mean: 67; SD: 18). Farmers were interrogated by a questionnaire about their main practices and basic mastitis knowledge questions. Quarters and severity were recorded, sampled for bacteriology at day 0 and day 21 during 3 months. Individual cell counts were also recorded. Risk analysis included Odds ratio calculation (OR) and multivariate regression. The average prevalence of mastitis on the study was 3.25% (min 0.3%, max : 11.2%, SD : 2.8%) which is coherent with previous epidemiological studies in Wallonia. 124 quarters were sampled and non-negative bacteriology shown 45% of strictly environmental pathogens, 22% of strictly contagious pathogens and 33% of mix epidemiological model pathogens. Overall risk assessment revealed that cubicles free-stall herds shown mastitis lower incidence (OR = 0.49; IC95 [0.34-0.72]). Surprisingly, post-dipping appeared as an overall positive risk indicator for mastitis prevalence (OR =2.13; IC95 [2,13-3,39]). The germ specific approach revealed that Post-dipping was present in low incidence herds with contagious models (OR=0.29; IC95 [0.12-0.71]) and that the type of pathogen model is an interaction in post-dipping risk analysis (Breslow-Day test p<0.0001), whereas housing had no interaction with the model (p=0.33). In this way of categorizing variables, one is able to identify overall risk indicators or germ-specific model. This can lead to more accurate advices in farm consulting. [less ▲] Detailed reference viewed: 31 (3 ULg) POLLEN DISPERSAL ABILITIES OF ALS TARGET-SITE RESISTANT BLACK-GRASS (ALOPECURUS MYOSUROIDES HUDS.)Maréchal, Pierre-Yves ; ; Vancutsem, Françoise et alPoster (2011, September 05) Target-Site Resistant (TSR) black-grass individuals were introduced in a field, at different growing stages to be synchronised with autochthonous population. Patches of susceptible black-grass were ... [more ▼] Target-Site Resistant (TSR) black-grass individuals were introduced in a field, at different growing stages to be synchronised with autochthonous population. Patches of susceptible black-grass were created by setting protective canvas on few areas (from 0,5 up to 32m²), during herbicide spraying and they were removed afterwards. Those patches were disposed along rays starting from the introduction point at distances from 2 to 128 meters. In those patches, local black-grass grew regularly and was able to receive some pollen from the resistant individuals, located in the centered emitting area. The herbicide field treatment (mesosulfuron+iodosulfuron : 15+3 g.a.i.ha-1) was efficient, confirming that the local population is susceptible to ALS inhibitors, was preceded by another herbicide treatment targeting only broad-leaves weeds, performed on the entire field, including patches, such as the rest of cultural practices such as fertilization, and fungicide and insecticide treatment. All seeds were harvested in each patches separately and tested in glasshouse. Seed samples were sprayed with sulfometuron, herbicide active ingredient known not to be affected by non-target site resistance, in a way to detect the percentage of resistant black-grass that can be engendered after the introduction of TSR individuals and to determine the distance resistant pollen can cover. DNA analyses were performed on surviving plants to confirm the presence of the mutation. [less ▲] Detailed reference viewed: 15 (0 ULg) Glucose-responsive layer-by-layer microcapsules as self-regulated insulin delivery systemAlaimo, David ; Detrembleur, Christophe ; et alPoster (2011, September 03) Detailed reference viewed: 16 (1 ULg) Disulfide bridges, new prospect in drug delivery systems?Cajot, Sébastien ; ; Schol, Daureen et alPoster (2011, September 03) Detailed reference viewed: 18 (5 ULg) Smart block copolymers for biomedical applicationsSibret, Pierre ; ; De Pauw, Marie-Claire et alPoster (2011, September 03) Stimuli-responsive polymers are polymers that respond with rapid changes to external stimuli such as pH, temperature, light or ionic strength. Responses to the stimuli may manifest themselves as changes ... [more ▼] Stimuli-responsive polymers are polymers that respond with rapid changes to external stimuli such as pH, temperature, light or ionic strength. Responses to the stimuli may manifest themselves as changes in solubility, shape or surface characteristics. They can also lead to the fomation of micelles or a sol-gel transition. These materials are very intersesting for different biomedical applications such as drug delivery systems, tissue engineering or sensors. In this work, we focused on two separate systems: on the one hand, micelles and, on the other hand, iron oxide nanoparticles. These nanoparticles are generally synthesized in a one-step process by alkaline coprecipitation of iron (II) and iron (III) precursors in aqueous solutions (Massart process). However, iron oxide nanoparticle suspensions produced by Massart process are not stable enough in physiological conditions to be used as is. A stabiliser coating is needed to avoid aggregation and consequent precipitation of the colloids in body fluids. For this coating, the polymer blocks chosen are: the poly(ethylene oxide) (PEO), the poly(acrylic acid) (PAA) and the poly(N-isopropyl acrylamide) (PNIPAM). The high flexibility and hydrophilicity of PEO chains make it an outstanding candidate for confering stealthiness to micelles and nanoparticles in order to avoid their rapid removal from the body by the opsonization process. The PAA is the pH-responsive block and the anchoring block. The PNIPAM is the thermoresponsive block with a thermal transition close to 37°C (99°F). Triblock copolymer was synthesized by a Reversible Addition Fragmentation Transfer Polymerization (RAFT) process combining poly(acrylic acid) PAA, poly(N-isopropylacrylamide) and poly(ethylene oxide) or poly[acrylate methoxy poly(ethylene oxide)]. This triblock copolymer was used alone to form micelles and with iron oxide to make magnetic stabilized nanoparticles. The behaviour of micelles and coated nanoparticles was investigated in different conditions by a combination of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential measurements. Moreover, PAA-b-PNIPAM-b-PAMPEO nanofibers were obtained using electrospinning technique. These nanofibers present interesting prospects in the field of biomaterials and biomedical applications as they mimic the extracellular matrix of the skin. [less ▲] Detailed reference viewed: 18 (1 ULg) Voice Impact of a Prolonged Reading Task at Two Intensity Levels: Subjective Self-RatingsRemacle, Angélique ; FINCK, Camille ; et alPoster (2011, September 01) Objectives: The objectives of this study are to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading, using subjective self-ratings. Methods: Fifty ... [more ▼] Objectives: The objectives of this study are to evaluate the impact on voice of both duration and intensity level of 2 hours of continuous oral reading, using subjective self-ratings. Methods: Fifty normophonic women undergo two sessions of voice loading in which the required intensity level of voice varied: 60-65 dB(A) for the first session, and 70-75 dB(A) for the second session. Voice intensity level is constantly controlled with a Digital Sound Level Meter at a distance of 40cm from the mouth. Subjects’ voices are orally loaded by reading a novel of their choice for 2 hours in a quiet room (background level < 30dBA). The effects of loading are assessed with regard to self-ratings of voice obtained before and every 30 minutes during the loading tasks. The following parameters are evaluated using a 100-mm horizontal visual analogue scale: voice quality, phonation effort, vocal fatigue and laryngeal discomfort. A repeated-measure analysis of variance (ANOVA Duration X Intensity Level) where the subjects are used as their own controls is carried out to compare data obtained at different times of the reading sessions. Results and conclusion: There is a significant effect of the duration (p < 0.0001) for all the subjective self-ratings, but no differences between the two intensity levels of reading. Feeling of phonation effort, vocal fatigue and laryngeal discomfort significantly increase during prolonged reading, whereas self-rating of voice quality decrease. Subjects do not report more complaints in the 70-75 dB session than in the 60-65 dB one. To conclude, subjects’ self-ratings of voice significantly worsens throughout the reading task as a duration effect, but there is no intensity level effect. [less ▲] Detailed reference viewed: 14 (2 ULg) Automated synthesis of [18F] FBEM for labeling of thiol containing compoundsParis, Jérôme ; Thonon, David ; Kaisin, Geoffroy et alPoster (2011, September 01) [18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the ... [more ▼] [18F]FBEM, i.e. N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide, is a useful synthon employed for the specific radiolabeling of thiol containing compounds, including peptides and proteins. The aim of the present work was to develop a fast, reproducible and fully automated synthesis of this compound in order to improve its availabilty as well as for obvious radioprotection matters. [less ▲] Detailed reference viewed: 52 (13 ULg) Direct conversion of alcohol into azide : Drastically shortening the radiosynthesis of 4-azidomethyl-[18F]fluorobenzeneKaisin, Geoffroy ; Paris, Jérôme ; Flagothier, Jessica et alPoster (2011, September 01) Detailed reference viewed: 36 (12 ULg) Is there a link between an unstable identity and autobiographical memory in schizophrenia ?Blairy, Sylvie ; Dethier, Marie ; Boulanger, Marie ![]() Poster (2011, September) Detailed reference viewed: 45 (2 ULg) Tetanus following castration in a buckLamain, Guillaume ; Guyot, Hugues ![]() Poster (2011, September) Detailed reference viewed: 20 (5 ULg) A method for evaluating weaknesses and critical steps in the Radiation Treatment Process through Precursor Events reportingLENAERTS, Eric ; DELGAUDINE, Marie ; COUCKE, Philippe ![]() Poster (2011, September) objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains ... [more ▼] objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains, functional Basic Responsibilities and levels of Severity of precursor events [less ▲] Detailed reference viewed: 3 (1 ULg) Microbiological and chemical characterisation of St Anne cave, BelgiumCarnol, Monique ; Malchair, Sandrine ![]() Poster (2011, September) In Belgium, most drinking water is provided by calcareous karst aquifers. Chemical and microbiological characterisation of these systems focalises mainly on the transfer of pollutants and microbial ... [more ▼] In Belgium, most drinking water is provided by calcareous karst aquifers. Chemical and microbiological characterisation of these systems focalises mainly on the transfer of pollutants and microbial contaminants, major sources of sanitary risks. These studies are generally based on bacterial cultures, representing however only 1% of bacterial species present in the environment. Molecular techniques allow the study of the global microbial diversity of an environment, as they are based on direct ADN extractions, without previous culturing steps. The objective of this research was the study of the microbial diversity in the ‘St Anne’ cave, Belgium. Chemical composition of the water, cultivable bacteria and the diversity of ammonia-oxydizing bacteria (AOB) were studied in waters and sediments of the ‘Chawresse’ (underground river in St Anne), on the cave’s wall and in soils aboveground. Bacterial counts revealed that most cultivable bacteria were associated with suspended particles and that their numbers decreased underground. Molecular analyses revealed the presence of AOB in the karst system. AOB are responsible for the first, acidifying step of the nitrification process. Further studies will specify and quantify their activity in this karst system. [less ▲] Detailed reference viewed: 56 (11 ULg) Optimized deposition of photocatalytic TiO2 thin films produced by a non-aqueous sol-gel methodMalengreaux, Charline ; ; Pirard, Sophie et alPoster (2011, September) Detailed reference viewed: 32 (11 ULg) Assessing the impacts of technical uncertainty on coupled surface/subsurface flow model predictions using a complex synthetic caseWildemeersch, Samuel ; Goderniaux, Pascal ; Orban, Philippe et alPoster (2011, September) According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful ... [more ▼] According to the EU Water Framework Directive, Member States have to manage surface water and groundwater at the water body scale and in an integrated way. Flow and transport models constitute useful management tools in this context since they can predict system responses to future stresses. However, numerical modelling at such a scale faces specific issues linked to (1) the representation of the geological and hydrogeological complexity, (2) the uneven level of characterisation knowledge, (3) the representativity of measured parameters and variables in the field, and (4) the CPU time needed for solving the numerical problem. Assumptions and simplifications made for dealing with these issues can lead to a series of models differing by their complexity and by the reliability of their predictions. Consequently, modellers have to find a compromise between complexity and reliability. The main objective of this research is to estimate the impacts of technical uncertainty, which is the uncertainty related to the numerical implementation, on groundwater flow model predictions. To reach that objective, the methodology consists in comparing reference predictions (hydraulic heads and flow rates) of a complex and close to reality synthetic case with the predictions provided by a series of simplified models (coarse spatial discretisation, coarse time discretisation, simplified law in the unsaturated zone). The synthetic case reflects the main characteristics found in groundwater bodies of South Belgium (Condroz region of Wallonia), characterised by a succession of limestone synclines and sandstone anticlines. The numerical model is developed with the fully-integrated surface/subsurface flow and transport code HydroGeoSphere using a mesh refined along the surface water network (153027 nodes and 269872 elements). A 5-year reference transient simulation, with daily stress factors is performed. The simulated hydraulic heads and flow rates constitute the reference observations and predictions for the comparison with the simplified models. The simplified models tested differ by their horizontal (500 m vs. 1000 m element size) and vertical (8 layers vs. 3 layers) spatial discretisations, their time discretisation (daily vs. monthly stress factors), and the type of constitutive law used for simulating the unsaturated flow (linear vs. van Genuchten). The models are run with the same parameter values than those used in the reference model to evaluate the deterioration in model predictions due to technical uncertainty. Additionally, some of the models are calibrated with the inverse modelling code PEST to distinguish how far a model calibration can possibly compensate for technical uncertainty. Then, predictions from each simplified model are compared with the reference predictions of the synthetic case. Then, the simplified models are ranked using several model performance criteria. Results of this research provide guidelines for the numerical implementation of groundwater flow models at the water body scale with respect to specific groundwater management objectives. [less ▲] Detailed reference viewed: 32 (13 ULg) NEONATAL INVASIVE GROUP B STREPTOCOCCAL (GBS) INFECTIONS IN EUROPEMELIN, Pierrette ; ; et alPoster (2011, September) Objectives: To describe clinical characteristics and capsular type of GBS isolates responsible of invasive infections in infants from Belgium, Bulgaria, Czech-Republic, Denmark, Germany, Italy, Spain and ... [more ▼] Objectives: To describe clinical characteristics and capsular type of GBS isolates responsible of invasive infections in infants from Belgium, Bulgaria, Czech-Republic, Denmark, Germany, Italy, Spain and United Kingdom, representing one of the main objectives of the DEVANI (DEsign of a Vaccine Against Neonatal Infections) project. Methods: Surveillance of invasive GBS infections in infants was performed from mid-2008 through December 2010. For each case, a standardized case report form was filled. Samples from cases were processed using local procedures. GBS isolates were characterised in national central labs using standardised type-specific (Ia, Ib-IX) latex agglutination and molecular typing methods. Results: Data on 188 infants with invasive infection were analysed: 144 (60.6%) early onset diseases (EOD) and 74 (39.4%) late onset diseases (LOD). In EOD, mean/median ages at onset were 14/0 hours and the male:female ratio was 1.25. The predominant manifestation at onset was respiratory distress (42% cases); 83% cases were associated with sepsis/bacteremia, 15% with pneumonia and 6% with meningitis. Late-prenatal screening cultures were obtained from 51% of cases’ mothers and only half of these were positive for GBS. Non-elective C-section, intrapartum fever and rupture of membrane (>18h) were more frequent in EO-cases’ mothers versus healthy babies’ GBS-positive mothers. The major serotypes were III (43%), V (21%) and Ia (18%). In LOD, mean/median ages at onset were 42/34 days and the male:female ratio was 0.9. The predominant characteristic at onset was fever (62% cases); 70% cases were associated with sepsis and 30% with meningitis. Very rare manifestations were osteomyelitis and cellulitis. Serotype III was highly predominant (80.6%) followed mainly by Ia (12.5%). Death rates were 4.7/1.5% in EOD/LOD. Conclusions: Clinical presentations were associated with age at onset of infection. Serotype III predominated in neonatal infections. Prenatal screening was not universal neither sensitive. Study funded through the European Commission Seventh Framework. [less ▲] Detailed reference viewed: 2 (0 ULg) Tail amputation in a Belgian blue bullLamain, Guillaume ; Sartelet, Arnaud ; Touati, Kamal ![]() Poster (2011, September) Detailed reference viewed: 91 (31 ULg) Structural and morphological Control of TiO2 nanoparticles by selective adsorption of organic moleculesDufour, Fabien ; ; et alPoster (2011, September) Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde ... [more ▼] Semiconducting nanocrystals with tailored shapes have been widely investigated in the past decades because of their many shape-dependant properties. Anatase, a metastable phase of the titanium dioxyde (TiO2), is one of the most interesting material in many applications, such as photocatalysis, photovoltaics, photo/electrochromics or sensors. [1] In addition to an influence of the nanoparticles size, recent works on the anatase phase demonstrated the effect of the expressed surfaces nature on the nanoparticles photoreactivity. [2] Sol-gel method has been used to obtain a wide range of anatase nanoparticles sizes and morphologies by the control of concentrations, ions in solution, solution acidity and aging parameters. [3] In order to avoid the brookite phase often obtained with the anatase phase, microwave heating was efficiently used. Furthermore, conventional heating method has been compared with hydrothermal and microwave activated synthesis. The precise morphological control has been achieved by selective adsorption of organic molecules during synthesis: different shapes of anatase nanoparticles have been synthesized. [4,5]. The influences of synthesis parameters and the organic molecules nature on the nanoparticles structure and morphology have been analysed with various techniques, such as XRD and HRTEM. [less ▲] Detailed reference viewed: 32 (4 ULg) Successful reduction of cholelithiasis in a Holstein cowLamain, Guillaume ; Frisee, Vincent ; Ramery, Eve et alPoster (2011, September) Detailed reference viewed: 20 (5 ULg) Origine de peuplements forestiers tropicaux – Cas du tali (Erythrophleum suaveolens) et de l’assamela (Pericopsis elata) au sud-est du CamerounBourland, Nils ; ; Guion, Hélène et alPoster (2011, September) Detailed reference viewed: 45 (20 ULg) A new longirostrine ichthyosaur (Reptilia) from the Toarcian of France broadens the ecological diversity of the genus Temnodontosaurus; Fischer, Valentin ; et alPoster (2011, September) The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids ... [more ▼] The ichthyosaur genus Temnodontosaurus has always been viewed as a top predator of the Early Jurassic marine environments, while other contemporaneous ichthyosaurs such as leptonectids and stenopterygiids were occupying the lower trophic levels. We describe here an almost complete skeleton of this successful genus from the middle Toarcian (Lower Jurassic) of the Beaujolais foothills near Lyon, France, and assign it to a new species of Temnodontosaurus. This specimen exhibits cranial peculiarities such as a thin, elongated, and likely edentulous rostrum, as well as a reduced quadrate. Such morphological combination indicates dietary preferences that markedly differ from other species referred to as Temnodontosaurus. Despite a conservative postcranial skeleton, we propose that Temnodontosaurus is one of the most ecologically diverse genera of ichthyosaurs, including apex predators, small and soft prey longirostrine hunters, and generalized forms. Ammonites collected along the described specimen indicate that the new species is younger (bifrons ammonite zone) than most known Toarcian ichthyosaurs and therefore slightly postdates the severe environmental changes and marine invertebrate extinctions that occur during the Toarcian Oceanic Anoxic Event. The present study hence raises the question whether the speciation of Temnodontosaurus towards a new ecological niche, may have been a consequence of the post-crisis marine ecosystem reorganization. [less ▲] Detailed reference viewed: 91 (2 ULg) BUILDING THE QUALITY INTO THE POLYMERIC SOLID DOSAGE FORM MANUFACTURING LINE - CRITICAL QUALITY ATTRIBUTES EVALUATION WITH NIR AND RAMAN PAT TOOLS; Mantanus, Jérôme ; Rozet, Eric et alPoster (2011, September) Detailed reference viewed: 19 (6 ULg) Perinatal depression in adolescent mothers : a public health concernNoirhomme-Renard, Florence ; ; Gosset, Christiane ![]() Poster (2011, September) Detailed reference viewed: 48 (4 ULg) Is the seagrass Posidonia oceanica a reservoir of fecal indicator bacteria in the Mediterranean SeaPolain, Stéphanie ; ; Lepoint, Gilles et alPoster (2011, September) Detailed reference viewed: 24 (4 ULg) Impurity fingerprints for the identification of counterfeit medicines - a feasibility studySacré, Pierre-Yves ; ; et alPoster (2011, September) Detailed reference viewed: 11 (0 ULg) Ant-aphid mutualism - A question of microorganisms?Fischer, Christophe ; ; Duriaux, Adrien et alPoster (2011, September) It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection ... [more ▼] It is now long known that some ant and aphid species can present a mutualistic relationship, ants using aphid honeydew as sugar source and in exchange providing the aphid colony cleaning and protection. From a behavioral point of view, this phenomenon has been well studied, but its chemistry and semiochemical mechanisms are still largely unknown. This study aims to identify semiochemicals involved in the establishment of this relation and their sources, using both chemical and behavioral methods. Bioassays revealed that the greatest part of ant attraction toward aphid colonies is due to honeydew volatile compounds; enabling ant scouts to find more quickly aphid colonies and distantly recognize myrmecophilous species. Many of those VOCs seeming to have microbial origins, the main honeydew microorganisms have been isolated and their roles in VOCs production and ant attraction have been investigated. [less ▲] Detailed reference viewed: 43 (17 ULg) Development of a quantitative risk assessment for cheese made from raw goat milk contaminated by Listeria monocytogenesDelhalle, Laurent ; ; Clinquart, Antoine et alPoster (2011, September) A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal ... [more ▼] A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal Agency for the Safety of the Food Chain in 2005. The source of the contamination was related to a shedder goat, excreting 2.6 log cfu (colonies forming units) L. monocytogenes / ml without any clinical symptom. On the basis of the collected data, a quantitative microbial risk assessment model was developed covering the production chain from the milking of goats until the consumed products. Predictive microbiology models were used to simulate the growth of L. monocytogenes during the process of cheeses made from goat raw milk. The modular exposure assessment model showed a significant growth of L. monocytogenes during chilling and storage of the milk collected the day before the cheese production (increase of 1.7 log cfu/ml for the median) and during the step of starter and rennet adjunction to milk (increase of 0.8 log cfu/ml for the median). The median estimated final result (in the fresh cheese) was equal to 3.5 log cfu/g. The model estimates (expressed as median final result issued from the exposure assessment) were realistic compared to the number of L. monocytogenes measured in the fresh cheese (3.6 log cfu/g) reported during the cheese contamination period. The average number of expected cases of human listeriosis was between 0 and 1 for a high-risk sub-population and 0 for a low-risk healthy sub-population. Scenario analysis was finally performed to identify the most significant factors and aid in developing priorities for risk mitigation. Thus, by using quantitative risk assessment and predictive microbiology models, this study provided valuable information to identify and to control critical steps in a local production chain of goat cheese made from raw milk. [less ▲] Detailed reference viewed: 17 (3 ULg) Enzymatic interesterification of palm oil and fractions: monitoring the degree of interesterification using different methods.; Danthine, Sabine ; et alPoster (2011, September) Detailed reference viewed: 6 (0 ULg) Evaluation of calcareous grasslands restoration success: density and reproductive success of specialist species.Harzé, Mélanie ; Piqueray, Julien ; Mahy, Grégory et alPoster (2011, September) Detailed reference viewed: 25 (14 ULg) Time and space matter when monitoring faecal pollution : searching for efficient sampling designs in relation to hydrological regimes.; ; et al Poster (2011, September) Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event ... [more ▼] Luxembourg (2586 km2) is a rural country with rather small urban areas. Except for one main river basin, the contamination of surface waters and groundwater by faecal material is an episodic event. Therefore, classical monitoring schemes based on low, fixed sampling frequency turned out to inefficiently estimate the microbial water quality. Several studies have therefore been carried out on important drinking water resources (springs and surface reservoir) in order to understand the link between the faecal contamination events and the hydrology and the hydrology of these waterbodies. Physico-chemical and hydrological variables that could serve as relevant indicators for triggering selective sampling were also investigated. [less ▲] Detailed reference viewed: 3 (1 ULg) Physicochemical properties of fat blends based on anhydrous milk fat and palm oil.Danthine, Sabine ![]() Poster (2011, September) Detailed reference viewed: 1 (0 ULg) Effect of Deferoxamine Mesylate on Freezability of Blood Supplemented Canine SemenVan Den Berghe, Femke ; ; et alPoster (2011, September) Detailed reference viewed: 41 (17 ULg) Pili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project); ; et al Poster (2011, September) Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI ... [more ▼] Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI (Design of a Vaccine Against Neonatal Infection) project. Methods GBS isolates from pregnant women (PW) and cases of newborn infection (NI) were collected in 8 European countries (Belgium, Bulgaria, Czech Republic, Denmark, Germany, Italy, Spain, United Kingdom) during 2009/10 under the auspices of DEVANI. Total no. of strains examined was 1078 and 192 from PW and NI, respectively. Isolates were screened by multiplex PCR and FACS analysis to evaluate respectively gene presence and surface-exposure of pili. Results The most common gene patterns found were PI-2a alone, PI 1+2a and PI 1+2b, while the PI-2b gene alone was very rare. The most prominent result was that a majority of isolates from NI carried the PI-1+2b gene pattern, while the most common pattern among PW was PI-1+2a. Most of analyzed strains express at least one pilus on their surface. Conclusions All isolates contained at least one gene coding for pili. When present pili 2a and 2b were highly surface exposed. [less ▲] Detailed reference viewed: 15 (0 ULg) Systematic expression profiling of Hox genes in the central nervous system of adult mouse; ; Coste, Cécile et alPoster (2011, September) Detailed reference viewed: 12 (0 ULg) GENERIC MEKC METHOD FOR THE SEPARATION OF 15 ANTIMALARIAL DRUGSLamalle, Caroline ; Marini Djang'Eing'A, Roland ; Debrus, Benjamin et alPoster (2011, September) Detailed reference viewed: 56 (18 ULg) Enzymatic Interesterificationof Palm oil and Fractions: A Calorimetric StudyDanthine, Sabine ; ; Lefebure, Emilie et alPoster (2011, September) Detailed reference viewed: 12 (5 ULg) Genetic diversity and population structure of the common dormouse, Muscardinus avellanarius, in Flanders region (Belgium)Mouton, Alice ; ; Michaux, Johan ![]() Poster (2011, September) Detailed reference viewed: 7 (0 ULg) Sedimentology of a continuous Givetian-Frasnian carbonate succession in Sauerland (Germany) and MS comparison with the time-equivalent ones of Ardennes (Belgium) and Moravia (Czech Republic)Pas, Damien ; Da Silva, Anne-Christine ; Boulvain, Frédéric et alPoster (2011, September) This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick ... [more ▼] This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg's quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick) covers a well constraint stratigraphic interval starting at the base of the Givetian (Stritzke 1991;Aboussalam et al., 2003) and according to our new datations ending within the Lower Famennian. The Middle-Upper Devonian shelf-edge within the Rheinisches Schiefergebirge can be traced from the supposed position along the southern rim of the Dinant Syncline and the Eifel Synclines, northwards along a line connecting the southern margin of the Devonian reefal outcrops of Attendorn and Brilon (Krebs 1967, 1974). The depositional setting of the investigated section corresponds to complex slope and basinal environments where reworked material from the proximal Brilon platform (located to the north) and basin deposits coexist. Thus, this section allows to follow the evolution of the Givetian-Frasnian Brilon (e.g., Machel 1990; Stritze 1990, 1991) platform in a deeper setting. Petrographic analysis of more than 300 thin-sections leads to the identification of 9 microfacies which are integrated into a palaeoenvironmental model. Microfacies curve evolution shows two main trends. A shallowing upward trend ending within a typical proximal slope setting (dismantling of the platform) followed by a deepening upward trend which is characterized by several meter of pelagic mudstone within the upper part of the studied section. Magnetic susceptibility variations in sedimentary rocks, have commonly been interpreted as related to variations of detritic inputs through climatic or sea level changes (Crick et al., 1994). The magnetic susceptibility (MS) study of more than 330 samples from this long-time fore-reef carbonated succession is an opportunity to better constrain our sedimentological interpretations. To do so, we propose a comparison between general MS trends and some parameters such as microfacies and relative sea level fluctuations interpreted on the basis of the sedimentological study. The relatively long stratigraphic interval covered by the Burgberg section offers a good opportunity to compare our data with the time equivalent Devonian sections of the Ardennes (Belgium) and Moravian karst area (Czech Republic) (Boulvain et al., 2010). And thus to test the magnetic susceptibility tool for long distance correlation between stratigraphically well constraint sections. [less ▲] Detailed reference viewed: 18 (5 ULg) Computed tomography diagnosis of pituitary abscess in a dairy cowGuyot, Hugues ; Michiels, Marie-Pierre ; Dahmani, Abdelkadir et alPoster (2011, September) Detailed reference viewed: 44 (21 ULg) Enhancement of in-situ Transformation of M2C Eutectics Carbides during High Temperature Heat Treatment on a HSS and a Semi-HSS GradesTchuindjang, Jérôme Tchoufack ; ; Lecomte-Beckers, Jacqueline ![]() Poster (2011, September) Alloys of the complex system Fe-Si-C-Cr-C-X, where X is a strong carbide forming-element are well known to exhibit interesting mechanical properties, including wear and abrasion resistances. Such a ... [more ▼] Alloys of the complex system Fe-Si-C-Cr-C-X, where X is a strong carbide forming-element are well known to exhibit interesting mechanical properties, including wear and abrasion resistances. Such a tribological behavior is mainly due to the presence of carbides especially those obtained during the solidification route and that are known as primary or eutectic carbides. It may therefore be interesting to determine the relative stability of primary carbides when considering thermal and thermomechanical treatments performed at a temperature high enough to allow either the homogenization of the matrix or the occurrence of a desired grain size. This thermal stage is often required to produce tailored microstructures that can lead to improved mechanical properties. In this work a series of thermal treatments performed on samples originated form casting foundry parts were done. Raw materials are both HSS and semi-HSS grades used in application where wear resistance is needed. Thermo- Calc® (TC) simulations and Differential Thermal Analysis (DTA) were performed to determine the crystallization behavior and the subsequent solid state transformations of the studied alloys respectively in equilibrium and in non equilibrium conditions. Light and Scanning Electron Microscopies were done together with hardness measurements in order to enhance metallurgical features of the heat treated samples. Image analysis yielded the determination of carbides volume fractions. It appears from microstructural analyses and carbides quantification that Mo-rich eutectic carbides undergo in situ phase transformations during heat treatments. In fact Mo-rich M2C carbides transform themselves into MC, M6C and M3C, through a so-called budding phenomenon. Such a phenomenon is the evidence of a preferential migration of some atoms that escape from the parent M2C carbide to diffuse further away from their initial site with increasing time and temperature. The stable or metastable nature of eutectic carbides is also discussed from DTA and TC results, as M2C carbides found in both as-conditions and DTA samples were not predicted by equilibrium conditions. [less ▲] Detailed reference viewed: 27 (6 ULg) Kinetics and diffusional limitations in nanostructured heterogeneous catalyst with controlled pore texturePirard, Sophie ; ; et alPoster (2011, September) Detailed reference viewed: 3 (0 ULg) Effeti comportamentali dell'endosulfan su girini di Rana dalmatina; ; et al Poster (2011, September) Detailed reference viewed: 7 (0 ULg) A BAYESIAN PREDICTIVE PROBABILITY CRITERION TO ASSESS ANALYTICAL METHODS VALIDITYRozet, Eric ; Marini Djang'Eing'A, Roland ; Lebrun, Pierre et alPoster (2011, September) Detailed reference viewed: 56 (16 ULg) Seagrass response to in situ trace element contaminationsRichir, Jonathan ; ; Lepoint, Gilles et alPoster (2011, September) The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of ... [more ▼] The Mediterranean seagrass Posidonia oceanica (L.) Delile has been widely used since the mid-seventies as bioindicator to monitor trace element (TE) coastal pollution. However, there is a lack of knowledge regarding to that seagrass contamination and decontamination kinetics. For the first time, Posidonia were experimentally in situ contaminated in 2009, at 10m depth in Calvi Bay (Corsica), by a mix of 15 TE (Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Ag, Cd, Pb and Bi). Trace element concentrations were measured by DRC-ICP-MS in leaves, rhizomes and in overall shoots sampled at regular time intervals. P. oceanica immediately accumulated pollutants from the beginning of experiments; once contaminations ended, TE concentrations came back close to their original levels within 2 weeks, or at least showed a clear decrease. Posidonia leaves exhibited different uptake kinetics for many elements (Cr, Cu, Zn, Ag, Cd, Pb and Bi): the younger growing leaves incorporated chemicals more rapidly than the older ones. Rhizomes did not show any clear trend, except for Cu, Zn and Bi. These results demonstrate that Posidonia is a very sensitive sentinel to immediately delineate punctual pollutions similar to what might be measured in contaminated Mediterranean waters. The very good response of Posidonia leaves to pollutant short term expositions suggests their routine use in regularly scheduled monitoring programs. Nevertheless, to by-pass Posidonia leaves deciduous character and their capability to detoxify rapidly, long term accumulation recordings also necessitate below-ground tissues analyses. [less ▲] Detailed reference viewed: 45 (4 ULg) Influence of palm oil enzymatic interesterificationon physicochemical properties of ternary fat blends involving anhydrous milk fatLefebure, Emilie ; ; Danthine, Sabine ![]() Poster (2011, September) Detailed reference viewed: 17 (6 ULg) When MIR spectrometry helps to promote a local and vulnerable breedColinet, Frédéric ; ; et alPoster (2011, August 31) The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of ... [more ▼] The dual purpose Belgian Blue breed (DP-BB) is a vulnerable breed rooted in the tradition of the Walloon Region of Belgium. DP-BB has interesting features (e.g. robustness, good longevity and ease of calving). Due to its dual purpose type, income generated by both milk and meat is more stable and more flexible in responding to market fluctuations. Registered DP-BB cows are milk recorded (one of the conditions for them to be registered as DP-BB and therefore to get AEM subsidies). Since near 4 years, during routine milk recording, nearly all mid-infrared (MIR) spectra generated at the milk labs and the information of test-day records were collected in a database. Calibration equations using the MIR spectrometry were developed permitting the prediction of several bovine milk components (e.g., fatty acids (FA)). Their application on the MIR spectral database would allow comparing milk composition from 920 DP-BB and 52,497 Holstein cows (selected cows had a proportion of Holstein or DP-BB in their breed composition of at least 90%). On average, each cows had 6 test-day records with MIR spectra in the database. MIR predictions were analyzed using GLM procedure with 5 fixed effects (breed, herd, lactation number, month of test-day recording and lactation stage); values presented are lsmeans (± s.e.) of the breed. Although milk and fat yields were lower for DP-BB, their FA proportions in fat were different from Holstein. Indeed, there are 66.0% (0.2) and 67.6% (0.0) of saturated FA in fat of milk for DP-BB and Holstein, respectively. Furthermore, the DP-BB milk fat was richer in omega-9 (20.7% (0.2) vs. 19.6% (0.0) for DP-BB and Holstein, respectively). Use of MIR predictions may help stakeholders to promote milk and future dairy products from DP-BB. [less ▲] Detailed reference viewed: 22 (11 ULg) JUDE: A Far-UV Imager for JUICEGrodent, Denis ; ; et alPoster (2011, August 31) Detailed reference viewed: 30 (4 ULg) Integration of foreign breeding values for stallions into the Belgian genetic evaluation for jumping horsesVandenplas, Jérémie ; ; Gengler, Nicolas ![]() Poster (2011, August 30) The aim of this study was to test the integration of foreign estimated breeding values (EBV) for stallions into the Belgian genetic evaluation for jumping horses. Belgian breeders import horses from ... [more ▼] The aim of this study was to test the integration of foreign estimated breeding values (EBV) for stallions into the Belgian genetic evaluation for jumping horses. Belgian breeders import horses from neighbouring countries for which foreign information is needed as prior to estimate a more accurate EBV. The Belgian model is a bivariate repeatability BLUP animal model. For the year 2009, pedigree and data files contained 101 382 horses and 712 212 performances. 98 French and 67 Dutch stallions were selected and their foreign EBVs were converted into Belgian national trait. Associated reliabilities were also estimated. A Bayesian approach (Legarra et al., 2007) was applied to integrate this prior information into the Belgian evaluation. It led to a slight modification of the average EBV and of the standard deviation for the whole population. It also led to a new Belgian ranking of the foreign stallions more similar to foreign rankings. However, the adequacy of the Belgian model was not damaged. With regards to prediction ability, Bayesian evaluations using conversion equation estimated by Weighted Least Squares procedure predicted the best traditional EBVs of the year 2009 for the French stallions. For the Dutch ones, it were the evaluations associated to the conversion equation based on Wilmink et al. (1986). For both countries, Bayesian evaluations using conversion equation based on Goddard (1985) improved the most the stability of EBVs. Finally, integration of French and Dutch information improved reliabilities of the Bayesian EBVs of at least 5% and 2% for French and Dutch stallions, respectively. These results confirm the interest to integrate foreign information into the Belgian evaluation for jumping horses. [less ▲] Detailed reference viewed: 35 (9 ULg) Gender's best friend effect on depression in adolescenceBoulard, Aurore ; Born, Michel ![]() Poster (2011, August 26) The development of intimacy during the adolescence is considered by several authors as a central developmental task (Sharabany et al., 2008, Erber & Erber, 2001) and can be seen as a predictor of any ... [more ▼] The development of intimacy during the adolescence is considered by several authors as a central developmental task (Sharabany et al., 2008, Erber & Erber, 2001) and can be seen as a predictor of any psychosocial adjustment. The purpose of this study is to examine the relationship between peer attachment, parent attachment and depressive mood in adolescence. [less ▲] Detailed reference viewed: 24 (3 ULg) Stereocontrol of attosecond time-scale electron dynamics in ABCU using ultrafast laser pulses: a computational study.Mignolet, Benoît ; Remacle, Françoise ![]() Poster (2011, August 23) Detailed reference viewed: 10 (0 ULg) Potential effects of blood contaminants on immune responses in harbour seals (Phoca vitulina)Dupont, Aurélie ; ; et alPoster (2011, August 23) Detailed reference viewed: 29 (3 ULg) Detection of aggregative behaviour in binary choice experimentsBrostaux, Yves ![]() Poster (2011, August 22) Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments ... [more ▼] Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments. Analysis of these experiments reveals specific characteristics (unequal counts between repetitions, dependence of individual choices) that make the traditional adjustment tests fail. We demonstrate that the use of generalized linear models can circumvent these pitfalls and deliver a reliable diagnosis on the social behaviour of the studied invertebrates. The strength of this behaviour can then be evaluated through the use of common correlation models. [less ▲] Detailed reference viewed: 18 (8 ULg) Study of the interactions between the late stage proteins of Escherichia coli divisome assembly.Dony, Nicolas ; Brasseur, Robert ; Joris, Bernard ![]() Poster (2011, August 16) Detailed reference viewed: 33 (4 ULg) Use of Chrysoperla carnea larvae for biological control of immature stages of Leptinotarsa decemlineataSablon, Ludovic ; Lejeune, Renaud ; Haubruge, Eric et alPoster (2011, August 13) The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is an important economic pest of potato which populations are effectively controlled by insecticides. However, development of resistance ... [more ▼] The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is an important economic pest of potato which populations are effectively controlled by insecticides. However, development of resistance along with the poor image of unnaturally management methods pushes researchers to develop alternative "greener" methods. Chrysoperla carnea is a generalist predator whose larvae are very voracious on any types of soft-bodied insects. In laboratory assays, we demonstrated a good predatory potential of lacewing larvae against eggs, first and second CPB larval instars. The third lacewing larval instar showed an important consumption of CPB immature stages. Indeed, when looking at the daily consumption, we found that prey consumption by the third larval instar was 3-fold higher compared to the two first instars. Partial or total consumption of prey was also numbered. Different proportions of partial/total consumption were found and these depend on the lacewing larval stage. This study provides new perspectives for possible use of C. carnea as a biological agent to control CPB. Nevertheless, additional work has to be conducted under semi-natural and field to completely evaluate the lacewing potential as a CPB predator. [less ▲] Detailed reference viewed: 35 (8 ULg) Influence of temperature on conservability of chilled vacuum packed beef from different originsDidimo Imazaki, Pedro Henrique ; ; Nezer, Carine et alPoster (2011, August 07) The objective of this experiment was to study the conservability of chilled vacuum-packed meat depending on storage temperature (–1 °C vs. +4 °C) during the last third of their shelf life. Physicochemical ... [more ▼] The objective of this experiment was to study the conservability of chilled vacuum-packed meat depending on storage temperature (–1 °C vs. +4 °C) during the last third of their shelf life. Physicochemical parameters (pH and colour) and microbiological growth (total aerobic bacteria, lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp. and Brochothrix thermosphacta) of Longissimus dorsi samples from different origins (United Kingdom and Ireland, Australia and Brazil) were measured at: i) 2/3 of their shelf life and ii) the end of their shelf life. Sample bacteria population growing on MRS was identified by API 50 CHL strips. Unlike Irish and British samples, pH of some Australian and Brazilian samples decreased during conservation. The colour of the samples remained stable and it did not seem to be influenced by temperature. All samples conserved at –1 °C presented a satisfactory microbiological quality at the end of their shelf life (British and Irish meat = 35~45 days; Australian meat = 140 days and Brazilian meat = 120 days). On Australian and Brazilian samples, temperature did not influence total aerobic bacteria growth, but conservation at +4 °C favoured lactic acid bacteria and Enterobacteriaceae growth. API 50 CHL strip identifications revealed the presence of bacteria like Lactobacillus brevis, Carnobacterium maltaromaticum and Lactobacillus fermentum, which occur naturally in fresh meat and are known for their bioprotective effect against other microorganisms. Further analyses are being carried out using molecular methods in order to study the initial bacteria population diversity and it evolution during storage. [less ▲] Detailed reference viewed: 38 (13 ULg) Genome-wide epistasis screening for asthma associated traitsGusareva, Elena ; ; Van Steen, Kristel ![]() Poster (2011, August 01) Genome-wide association (GWA) studies of asthma and associated traits have identified numerous genes. A substantial portion of the heritability of these traits remains unexplained. Some variants, not ... [more ▼] Genome-wide association (GWA) studies of asthma and associated traits have identified numerous genes. A substantial portion of the heritability of these traits remains unexplained. Some variants, not detectable via main effects GWA study may manifest themselves only in interaction with other variants. To search for interacting genes involved in regulation of asthma associated traits (total IgE, eosinophils, FEV1, FVC, FEV1/FVC) we performed GWA epistasis screening in two family groups of asthma patients:CAMP (Childhood Asthma Management Program:814 cases and 467 trios) and CARE (Childhood Asthma Research and Education:796 cases and 338 trios) [dbGaP accession number phs000166.v1.p1.c1]. Individuals were genotyped with the Aymetrix 6.0 array. After quality control 574922 and 575010 SNPs in CAMP and CARE respectively, were tested with FBAT. No main effects genome-wide significant associations were found. We prioritized candidate pairs of SNPs for MB-MDR epistasis screening using Biofilter leading to 7632 SNPs for CAMP and 7603 SNPs for CARE. The most significant pair-wise interaction was identified between SNPs from loci 7p21.1 and 12q23.3 influencing eosinophil level in asthmatics. [less ▲] Detailed reference viewed: 46 (8 ULg) Ion heating in travelling-wave based ion mobility spectrometerMorsa, Denis ; De Pauw, Edwin ; Gabelica, Valérie ![]() Poster (2011, August 01) Detailed reference viewed: 2 (0 ULg) Evidence of a verbal overshadowing effect in childrenVanootighem, Valentine ; Brédart, Serge ; Dehon, Hedwige ![]() Poster (2011, August 01) The report of verbal descriptions of a culprit by adult witnesses may impair their later identification ability, a phenomenon known as the “verbal overshadowing effect (VO)” (Schooler & Englster-Schooler ... [more ▼] The report of verbal descriptions of a culprit by adult witnesses may impair their later identification ability, a phenomenon known as the “verbal overshadowing effect (VO)” (Schooler & Englster-Schooler, 1990). In spite of a large body of literature on the suggestibility of children testimony, only one study has examined whether descriptions also impaired children’s identification abilities in a single group of children (8-9 years old) and no evidence of VO was found (Memon & Rose, 2002). However, some procedural details were not controlled in this experiment and the absence of a control adult group did not allow determining whether the procedure used was able to induce a VO effect. Hence, 2 experiments were conducted on several groups of children (7-8, 10-11, 13-14 years old) and adults to determine the influence of development on the VO effect. Overall, a VO effect on face identification was found in both experiments. The quality and influence of descriptors across the ages were also examined. [less ▲] Detailed reference viewed: 41 (5 ULg) VHHs as model proteins to investigate amyloid fibril formation: effect of seeding and cross-seeding on the stability of fibrilsChavignon, Chloé ; ; et alPoster (2011, August) The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidoses are known so far, amongst ... [more ▼] The term "amyloidosis" covers a group of diseases associated with the deposition of protein aggregates organized into amyloid fibrils in different organs. About forty amyloidoses are known so far, amongst which Alzheimer's disease, type II diabetes and immunoglobulin amyloidosis [1]. Although the mechanism of amyloid fibril formation at the molecular level is not yet completely understood, it has been shown that the capacity to form amyloid fibrils in vitro is an intrinsic property of all polypeptide chains [1]. The choice of model proteins to investigate the aggregation process in vitro is therefore not restrained to proteins involved in amyloidoses but can be settled on a wide variety of proteins. In this study, we have chosen to investigate the mechanism of amyloid fibril formation by two variable domains of camelid heavy-chain antibodies (referred to as VHHs or nanobodies), cAb-HuL6 and cAb-BcII10, for which variants with mutations located at the disulfide bond [3,4] and the CDRs [3] are available. Characterisation of the aggregating properties of these mutants will allow the investigation of the impact of these structural elements on the process of fibril formation. In order to determine conditions in which cAb-HuL6 and cAb-BcII10 are more susceptible to form amyloid fibrils, heat-induced unfolding experiments at several pHs have been monitored by intrinsic fluorescence and circular dichroism. Then, aggregation experiments have been performed in the selected conditions and the presence of amyloid fibrils has been acknowledged by thioflavineT fluorescence experiments and electron microscopy. We will discuss the kinetics of aggregation obtained in the absence and the presence of seeding/cross-seeding and the stability of the formed fibrils. [1] Chiti and Dobson, Annu. Rev. Biochem., 75, 2006, 333-366 ; [2] Dumoulin et al., Protein Sci., 11, 2002, 500-515 ; [3] Saerens et al., J. Mol. Biol., 352, 2005, 597-607 ; [4] Saerens et al., J. Mol. Biol., 377, 2008, 478-488. [less ▲] Detailed reference viewed: 20 (2 ULg) Endotoxin-induced alterations in renal oxygen consumption: an ESR oximetry studyQuoilin, Caroline ; Grammenos, Angeliki ; et alPoster (2011, August) The kidney, one of the most injured organs in critically ill patients, is faced with unique challenges for molecular oxygen regulation. Recent research activities in the pathophysiological mechanism of ... [more ▼] The kidney, one of the most injured organs in critically ill patients, is faced with unique challenges for molecular oxygen regulation. Recent research activities in the pathophysiological mechanism of acute renal injury (ARI) emphasize the central role of hemodynamic and inflammatory events in septic shock. More particularly, two mechanisms have been postulated to explain the inability of the injured kidney to extract oxygen: tissue hypoxia and cellular energetic metabolism dysfunction. The present investigation was carried out to characterize the effects of bacterial endotoxin on the oxygen consumption of human tubular proximal cell line (PTC) by using the very sensitive electron spin resonance oximetry method. Oxygen consumption was shown to decrease quite markedly in cells treated with lipopolysaccharide (LPS) from 16.52 ± 2.51 (n=6) in the control group to: 12.94 ± 2.62 (n=3) in the short incubation time group (6h) and 10.86 ± 2.20 (n=3) in the long incubation time group (18h). This decrease in oxygen consumption in renal cells after LPS challenge may be in relation with a metabolic down-regulation. Renal energetic are deranged in sepsis not just because O2 delivery is impaired but perhaps also because the ability of cells to utilize available O2 is compromised. [less ▲] Detailed reference viewed: 47 (18 ULg) Assessing IT Innovations in US State Governments: a research framework; ; et al Poster (2011, August) Detailed reference viewed: 15 (2 ULg) Effet de la technique vocale sur la justesse en voix chantéeLarrouy, Pauline ; Morsomme, Dominique ![]() Poster (2011, August) Selon Dalla Bella et al. (2007, 2009), la population générale est capable de chanter juste une chanson populaire lorsque le tempo de la production est lent. Le chant est une activité universelle, présente ... [more ▼] Selon Dalla Bella et al. (2007, 2009), la population générale est capable de chanter juste une chanson populaire lorsque le tempo de la production est lent. Le chant est une activité universelle, présente dès le plus jeune âge, mais reste une tâche complexe mobilisant des habiletés physiques, cognitives et physiologiques. En travaillant leur voix, les chanteurs professionnels, tels des instrumentistes, renforcent ces habiletés et sont capables de modifier la qualité de leur production vocale. Dans cette étude, nous examinons l’effet de cette expertise vocale et plus particulièrement de la technique “lyrique” enseignée aux chanteurs d’opéra sur certaines caractéristiques telles que la hauteur absolue et le tempo. En ce qui concerne la justesse, les techniques d’analyse acoustique mises au point nous permettent d’observer les erreurs possibles en production vocale (Schön et al., 2004) dans un contexte mélodique. Nous avons constitué un groupe contrôle de 63 non chanteuses et un groupe expérimental de 15 chanteuses professionnelles. Les sujets ont chanté « Happy Birthday ». Les chanteuses professionnelles l’ont chanté une première fois sans technique vocale particulière (ST) et une seconde fois avec une technique vocale lyrique (AT). La partie stable de chaque voyelle a été moyennée (AudioSculpt) et analysée (OpenMusic) afin d’estimer la hauteur absolue et relative de chaque note. Les résultats montrent des différences significatives entre les groupes pour le tempo (F(2,88)=16,61, p<.001) et la hauteur absolue (F(2,88)=165,63, p<.001). Chez les non chanteuses, le nombre d’erreur diminue avec le tempo (r=.321, p=01). Chez les chanteuses professionnelles AT, nous observons le profil inverse (r=-.662, p=01) mais pas de corrélation significative chez les chanteuses professionnelles ST. Les premiers résultats confirment l’effet de la technique vocale tant sur le type d’erreur que sur l’importance de ces erreurs en contexte mélodique. Bien que nous attendions une bonne performance chez les chanteuses professionnelles AT, les résultats ne vont pas dans ce sens. Cela conduit à discuter le type d’analyse à exécuter et le choix des programmes pour le jugement de la justesse en voix chantée avec technique vocale. [less ▲] Detailed reference viewed: 79 (20 ULg) Promoter analysis of the three HMA4 copies in the zinc hyperaccumulator Arabidopsis halleriNouet, Cécile ; ; Motte, Patrick et alPoster (2011, August) Detailed reference viewed: 73 (7 ULg) Expression of the metal homeostasis gene FRD3 in two Arabidopsis speciesCharlier, Jean-Benoît ; Polese, Catherine ; et alPoster (2011, August) Detailed reference viewed: 22 (13 ULg) C FIBERS - MG MATRIX COMPOSITES PRODUCED BY SQUEEZE CASTING AND FRICTION STIR PROCESSING: Microstructure & Mechanical BehaviourMertens, Anne ; ; Poster (2011, August) Mg matrix composites appear as suitable competitors to Al alloys, as they allow for a significant weight reduction while exhibiting potentially comparable mechanical properties. A large variety of ... [more ▼] Mg matrix composites appear as suitable competitors to Al alloys, as they allow for a significant weight reduction while exhibiting potentially comparable mechanical properties. A large variety of processing routes have already been considered for their production, including 'liquid state' processes such as squeeze casting. However these techniques necessitate a very careful control, simultaneously, of the solidification process, the wetting behaviour and the possible interfacial reactions between the reinforcements and the molten metal. And this is more particularly true in the case of Mg matrix composites with carbon fibers reinforcements, as Mg alloys are known to exhibit a poor wetting behaviour on carbon substrates. As a consequence, 'solid state' processing routes such as friction stir processing, that has been developed more recently, provide promising alternatives for the production of C-Mg composites. In the present work, Mg-Al-Zn alloys have been reinforced with carbon fibers using squeeze casting and friction stir processing. The microstructures and mechanical behaviour of composites produced by both processes have then been characterised and compared to those of non-reinforced samples, with a particular attention to the damage mechanisms prevailing in the composite materials. As a result, both processes have been compared in terms of the properties of their final products and hence their ability to produce sound composites. [less ▲] Detailed reference viewed: 45 (5 ULg) Attraction of wireworms towards root-emitted volatile organic compounds of barleyBarsics, Fanny ; Latine, Rémi ; Haubruge, Eric et alPoster (2011, August) Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for ... [more ▼] Wireworms (Coleoptera: Elateridae) are soil dwelling pests of many crops. Aldrin and lindane used to provide satisfactory treatment levels of infested lands. Their withdrawal from the market for environmental issues gave birth to new needs in terms of management. In order to enhance integrated management of this pest, we work on the chemical ecology of Agriotes sordidus Illiger. We focus on its interaction with barley roots, mediated by volatile organic compounds (VOCs). Our experimentations are based on behavioural responses of wireworms in olfactometric devices and their statistical relevance [less ▲] Detailed reference viewed: 30 (12 ULg) Impact of the use of the Manufacturer's published reference range for PTH vs. the reference range established in the Laboratory for the classification of the haemodialyzed patients with the KDIGO Guidelines.CAVALIER, Etienne ; DELANAYE, Pierre ; Vranken, Laura et alPoster (2011, July 28) Detailed reference viewed: 22 (0 ULg) New insights on the stability of the (1-84) PTH as determined with an automated 3rd generation PTH assay.CAVALIER, Etienne ; CARLISI, Ignazia ; BEKAERT, Anne-Catherine et alPoster (2011, July 28) Detailed reference viewed: 21 (0 ULg) Analytical validation of Accu-check Inform II meters and strips before their use in the wards: practical experience of the CHU de Liège.CAVALIER, Etienne ; PONCIN, Joseph ; MUSSO, Giuseppe et alPoster (2011, July 27) Detailed reference viewed: 18 (4 ULg) Host location by the parasitoid, Nasonia vitripennis Walker (Hymenoptera : Pteromalidae)Frederickx, Christine ; Durieux, Delphine ; Verheggen, François et alPoster (2011, July 24) Successful reproduction for parasitoids encompasses a series of behavioral steps commonly defined as host-habitat location, host location, host acceptance and host regulation. Successful host location ... [more ▼] Successful reproduction for parasitoids encompasses a series of behavioral steps commonly defined as host-habitat location, host location, host acceptance and host regulation. Successful host location, where resources are patchily distributed within the environment, is dependent on the information value of stimuli used in the host location process. Chemical cues produced by either the host itself, products derived from the host play an important role in host location. This study investigated the role of odorant cues used during host location by the generalist parasitoid, Nasonia vitripennis Walker. N vitripennis is a common parasitoid of Dipteran pupae found in association with decaying carrion. The biological activity of eight of the volatile molecules constituting the odour of pupae were tested on the searching behavior of parasitoid females through chemoecological approache: olfactometry bioassays. [less ▲] Detailed reference viewed: 49 (13 ULg) Morphosyntactic disorders as a consequence of categorization difficulty in children with specific language impairmentLeroy, Sandrine ; ; et alPoster (2011, July 21) Detailed reference viewed: 41 (5 ULg) Glycine receptor activation influences early cortical developmentAvila Macaya, Ariel Salvatore ; Nguyen, Laurent ![]() Poster (2011, July 14) The strychnine-sensitive glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily. In the adult, the GlyR is known to mediate fast inhibitory neurotransmission in the spinal cord ... [more ▼] The strychnine-sensitive glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily. In the adult, the GlyR is known to mediate fast inhibitory neurotransmission in the spinal cord and in the brainstem. The GlyR has also been described in the embryonic cortex after embryonic day 19 (E19) (Flint et al., 1998) where it could participate in developmental processes, but its presence at earlier stages has not been documented. Since other neurotransmitter systems, i.e. GABA and its receptors, are known to be potent signals that control corticogenesis (Nguyen et al., 2001; Ik-Tsen et al., 2007), we wondered if glycine and its GlyR could also fulfill such a function. In this study, we analyze GlyR expression and its physiological function in the early development of the cortex using in vitro cultures of embryonic day 13 slices, patch-clamp and immunocytochemistry. [less ▲] Detailed reference viewed: 10 (3 ULg) Model of the Jovian magnetic field topology constrained by the Io auroral emissions; Bonfond, Bertrand ; Grodent, Denis et alPoster (2011, July 11) Detailed reference viewed: 1 (1 ULg) Cassini UVIS Observations of Varying Auroral Emissions on Saturn's Night Side; ; et al Poster (2011, July 11) Detailed reference viewed: 8 (3 ULg) Aphid - phytovirus interactions: investigation of virus binding mechanisms in insect vectors by lectin use and proteomic approachYattara, Almouner Ag Alhamis ; ; Francis, Frédéric ![]() Poster (2011, July 11) Aphids are well known for their role in virus transmission to host plant. In some cases, the virus is transmitted from plant to plant simply attached to the cuticule of the mouthparts or the forget. For ... [more ▼] Aphids are well known for their role in virus transmission to host plant. In some cases, the virus is transmitted from plant to plant simply attached to the cuticule of the mouthparts or the forget. For other, circulative virus transmission based on virion internalization through the aphid gut followed by transfer to salivary glands and finally to next plant during aphid feeding is required. In both situations, presence of receptor components through the digestive tract of the aphids is needed for virus binding and further transmission to next plants even if not localized at the same place. In order to investigate the specific binding of virus on particular aphid receptors, two aphid-virus models were selected to be tested using several lectins showing differential sugar binding specificities. Virus transmission efficacy assays with Myzus persicae and potato virus but also Acyrthosiphon pisum and pea enation mosaic virus were performed using a range of lectins to assess the potential competition of lectins and virus. Some interesting lectins were found to reduce the virus transmission with a 2 fold factor showing potential use of lectin in virus spread control. The aphids were also investigated by a proteomic approach using a two Dimension-Differential in Gel Electrophoresis (2D-Dige) coupled with mass spectrometry to determine the aphid proteins involved in virus transmissions. Head or digestive tubes of aphids were collected and investigated for non persistant or persistant virus models respectively. Differential abilities of aphids to transmit the selected virus models are discussed in relation with lectin affinity specificity and investigated aphid proteins found to be involved in vector-virus interactions. The application of lectin as potential way to reduce virus transmission by aphids will also be developed. 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