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See detailLaterality patterns for Gestalts of language
Dumitru, Magdalena ULiege; Van Calster, Laurens ULiege; Bouffier, Marion ULiege et al

Poster (2017, September 07)

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See detailProtein-polysaccharide complexes, a tool for protein delivery in CaCO3 microparticles
Ramalapa, Bathabile ULiege; Crasson, Oscar ULiege; Vandevenne, Marylène ULiege et al

Poster (2017, September 06)

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions ... [more ▼]

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions between proteins and particles making the encapsulation of proteins highly dependent on the pH of reaction solutions and the isoelectric point of the protein.1 This poses a great limitation on the successful loading of proteins into microparticles. In this study, we explored the use of polysaccharide-protein interactions to strongly enhance the encapsulation of proteins in CaCO3 microparticles. EXPERIMENTAL METHODS Previously, Vandevenne and colleagues2 inserted a human chitin binding domain (ChBD) that has intrinsic affinity for hyaluronic acid (HA) into β-lactamase (BlaP). This generated chimeric protein, named BlaPChBD, was shown to be fully bifunctional. In this study this hybrid protein (BlapChBD) was associated to HA and successfully loaded into CaCO3 microparticles using super critical CO2 technology aided by the templating effect of HA on CaCO3. Furthermore, thrombin cleavage sites were engineered on both sides of the inserted ChBD in the chimeric BlaP so that release of the protein from the microparticles could be easily achieved by protease cleavage. The microparticles were characterised for size, surface charge, poly-morphism and protein loading and in-vitro release assays were performed. RESULTS AND DISCUSSION The presence of ChBD inserted into the β-lactamase increased the encapsulation of the protein by 6-fold when complexed with HA (Fig. 1). In addition, we also showed that the encapsulated BlaP remains stable during this process using kinetic reaction of β-lactam hydrolysis. Our data showed that vaterite CaCO3 microparticles of sizes ranging between 4 and 7 µm were produced. We were also able to demonstrate that thrombin cleavage increased the release of the protein from the microparticles within 36 hours from <25% to 87% (Fig. 2). In conclusion, the presence of ChBD successfully improved the encapsulation yield of the protein while retaining up to 81% of its activity. CONCLUSION Protein-polysaccharide complexation demonstrates an excellent approach for the delivery of sensitive biomacromolecules which can otherwise be complicated due to electrostatic hindrances. Future prospects include using the methods we have developed for encapsulation of therapeutic proteins and using calcium carbonate as a carrier and scaffold in bone regeneration for example. [less ▲]

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See detailEnvironmental impact of food waste treatment in an EcoCleaner, a portable accelerated composter
Groslambert, Sylvie ULiege; Léonard, Angélique ULiege; Finet, Sébastien

Poster (2017, September 06)

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially ... [more ▼]

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially during storage. Different solutions exist to manage this organic waste, for instance local and centralized composting, anaerobic digestion, or co-incineration with municipal waste. Except for local composting, theses methods need to collect and transport food waste to be processed, which mean transporting a lot of water. The Life Cycle in Practice (LCiP) (LIFE12 ENV/FR/001113) project helps SMEs to reduce the environmental impacts of their products and services across the entire life cycle. Within the frame of this project, the environmental impact of the Eco-Cleaner system is evaluated with the standardized life cycle assessment (LCA) methodology. Eco-Cleaner (EC) is a stand alone accelerated system that can transform food waste in valuable compost in only 24 hours. It is designed by Biowaste Recycling (http://biowasterecycling.com/). It can be declined in different capacities, from family size (about 1-2 kg/days of food waste) to large communities of 1300 place settings (up to 700 kg/days). EC technology is based on a permanent control of the ratio of humidity and temperature to optimize fermentation rate of food waste by a special consortium of more than thirty thermophilic bacteria. Waste reduction is about 90% and compost is sanitized to ensure the destruction of active parasites, insect eggs and germs. EC compost is dry (80-90% siccity level), and it has a quite high nitrogen content (about 3-4% on dry matter) and is rich in organic matter. Thanks to an activated carbon filter (replaced every two years), it is odourless and has no noxious gas emission. Since it's dry, this compost can be stored without further degradation, and its transport has a reduced environmental cost. The functional unit is the composting of one ton of food waste. The inventory includes the machine itself, its use, its refitting every twelve years, and its end of life. Refurbishing includes mainly the replacement of the motor, the heating resistance, the plastic parts, the seals, and only 5% of metals. Avoided chemical fertilizers consecutive to compost application are considered as system expansion, and it induces negative impact values in all categories. Electricity for use is the main impacting element in all categories (BE mix) but this impact can be reduced by the use of on-site photovoltaic panels. Abiotic depletion is highly impacted due to the metals the EC is made of (92% of total weight, principally steel), but a large improvement is achieved by the quadruplicating of the life span because of refurbishing, and the recycling of metals at the end of life. Specific process data are processed in SimaPro 8.1 software, using Ecoinvent 3.1 database for additional generic data, and analysed with the CML IA 3.03 method. [less ▲]

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See detailComparison of the electrochemical performance of Li4Ti5O12 spinel as negative electrodes for lithium-ion batteries prepared by sol gel and spray drying methods
Mahmoud, Abdelfattah ULiege; Piffet, Caroline ULiege; Berardo, Loris ULiege et al

Poster (2017, September 05)

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high ... [more ▼]

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high energy efficiency. Most difficult challenges of the development of promising rechargeable batteries concern the electrode materials. Li4Ti5O12 (LTO) is one the most promising anode materials for Li-ion batteries, as it demonstrates very stable cycling stability and excellent safety. Its high operating potential (~1.5 V) allows to avoid the formation of SEI during the first cycle. The three-dimensional structure offers LTO excellent reversibility due to the near zero volume strain during the Li+ ion intercalation and deintercalation cycling. The main objective of this study on LTO samples was to evidence the effect of synthesis method and thermal conditions on their structural, morphological and electrochemical properties [1, 2]. The results demonstrate the strong influence of the synthesis route (Sol-Gel and spray-drying methods) and the thermal treatment on the capacity, cyclability and rate capability of the LTO spinel in Li-half-cell and Li-ion full-cell (see Figure 1). References [1] A. Mahmoud, J. M. Amarilla, K. Lasri, I. Saadoune, Electrochim. Acta 93 (2013) 163-172. [2] A. Mahmoud, J. M. Amarilla, I. Saadoune, Electrochim. Acta 163 (2015) 213-222. [less ▲]

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See detailSurface solar radiation modelling over 1900-2014: comparison between the regional climate model MAR and reanalyses
Wyard, Coraline ULiege; Doutreloup, Sébastien ULiege; Belleflamme, Alexandre ULiege et al

Poster (2017, September 04)

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere ... [more ▼]

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere. However, it is difficult to build strong conclusions before the 1950s because of the observations scarcity. The evolution of the surface solar radiation has been reconstructed over 1900-2014 using the regional model MAR (« Modèle Atmosphérique Régional ») which has recently been chosen to be part of the EURO-CORDEX project, thanks to the CORDEX.be project. Simulations were performed at a horizontal resolution of 5 km over a domain of 600 x 550 km² covering Belgium. Boundary conditions were provided by four reanalysis products: ERA-interim (1979-2014) completed by the ERA40 (1958-1978), NCEP/NCAR-v1 (1948-2014), ERA-20C (1900-2010) and 20CRV2C (1900-2010). Surface solar radiation measurements from the Global Energy Balance Archive and cloud cover observations from Belgocontrol covering 1966-2007 were used for the evaluation of the MAR model and the forcing reanalyses. Results show that MAR produces much better results than the reanalyses. The driving reanalyses can generate divergent trends while they assimilate observations and are supposed to represent the same climate. [less ▲]

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See detailLCM of construction waste towards circular economy of buildings: VALDEM project
Roy, Aubin; Groslambert, Sylvie ULiege; Pasquet, Vanessa et al

Poster (2017, September 04)

From resource prospective, building and construction sector is responsible for more than third of global resource consumption, including 12% of the fresh water use and its generation of solid waste is ... [more ▼]

From resource prospective, building and construction sector is responsible for more than third of global resource consumption, including 12% of the fresh water use and its generation of solid waste is estimated to be 40% of the total waste volume. At European level, construction and demolition waste is the largest waste stream representing one third of all waste produced in EU. Therefore the resource efficiency and management is crucial in building construction. A very significant part of the Construction and Demolition Waste (CDW) is not recycled today. Also a very limited part is used as a recycled content in the construction and building products and materials. This lack is mainly due to heterogeneity and dispersion of waste flows decreasing efficiency and economic viability of recycling. To address this issue, VALDEM project (funded by Interreg FWVL European Fund) aims to overcome barriers to increase up-cycling applications. The project focuses its activities in North of France, Flanders and Walloon regions (Belgium), and stands out from usual approaches by its cross-border view of circular economy. VALDEM aims, on one hand, to optimize buildings end of life management by developing new deconstruction, sorting and recycling processes to produce uniform and accessible material flows. On the other hand, the project aims at increasing recycling and generating high quality secondary materials (concrete and other flows) to be used in future buildings within an up-cycling prospective. And finally it aims at validating the solutions from technical, scientific, economic and environmental point of view. The Environmental assessment, based on LCA, consists of identifying hotspots and key aspects to prioritize the efforts of different economic actors. As a first step, an LCA meta-analysis is conducted to provide an environmental picture for different potential activities within the scope of the project. As a next step, a comparative LCA is conducted to assess the environmental benefits and impacts of different solutions proposed in the framework of the project in a decision making context, and to limit the impact transfer and to generate the maximum value for all the stakeholders. Finally, results will be transferred to main actors (recycling operators, buildings contractors, product manufacturers …) in the three regions in order to consolidate future key aspects to eco-design a building in light of circular economy. [less ▲]

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See detailA Techno-Functional Study of the Aterian Technocomplex at Ifri n'Ammar
Tomasso, Sonja ULiege; Rots, Veerle ULiege

Poster (2017, September)

The tanged tools of the Aterian technocomplex have generally formed the central point in debates on early indications of hafting (Clark, 1970). However, in order to obtain a true understanding of the ... [more ▼]

The tanged tools of the Aterian technocomplex have generally formed the central point in debates on early indications of hafting (Clark, 1970). However, in order to obtain a true understanding of the specificity of this technocomplex, it is essential to study entire assemblages and understand the characteristics of and the relation between the tanged and non-tanged tool component, both on a technological and on a functional level. Until today no functional studies were yet undertaken on complete Aterian tool assemblages. We present the results of the technological and use-wear analysis of the non-tanged tool component of the Aterian assemblage of Ifri n’Ammar (Morocco) and focus on aspects of tool use and hafting. The rock shelter of Ifri n’Ammar, located in the eastern Moroccan Rif, is known for its rich stratified lithic assemblages, dating to 83 ± 6 ka to 130 ± 8 ka for the upper levels and to 145 ± 9 to 171 ± 12 ka for the lower levels (Richter et al., 2010). In the framework of the recently published dating results of other maghrebian sites (Dörschner et al., 2016), Ifri n’Ammar takes an important position within discussions on the chronological attribution of the Aterian: a large time span extended from MIS 6 to MIS 3 [3]. The sequence has provided a large diversity of tool morphologies which offer the possibility to contribute to the discussion about use and hafting of tanged and non-tanged tools. Aside from the tanged tools and foliates, the material from comparable Aterian assemblages can be defined by the presence of side scrapers, end-scrapers, denticulates, such as blades, bladelets or Levallois cores (Bouzouggar and Barton, 2012). Despite the frequent presence of post-depositional surface alterations, reliable functional interpretations could be identified on a large number of tools. Previous studies had already confirmed that the tanged tools were used while hafted for hunting and animal processing activities (Tomasso and Rots, 2017). Also a variety of tasks and gestures could be identified on the non-tanged tools based on a combination of diagnostic macro- and microscopic wear traces. Interestingly, also the non-tanged tool component shows evidence of hafting, indicating the existence of a variety in hafting techniques. The integration of the functional results on the non-tanged and tanged tool component allows addressing questions on tool variability, diversity in tool morphology and site function. We argue that the results highlight the importance of the site for improving our understanding of the North African MSA. [less ▲]

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See detailUltrasonic spray deposition of electrochromic oxide films
Maho, Anthony ULiege; Manceriu, Laura ULiege; Colson, Pierre ULiege et al

Poster (2017, September)

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See detailPlantes invasives dans les carrieres europeennes : quelle est la situation ?
Monty, Arnaud ULiege; Ortmans, William; Jorion, Alexis et al

Poster (2017, September)

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See detailSynthesis, characterization, and durability study of Pt-Co hollow nanoparticles deposited on carbon xerogel electrocatalysts for Proton Exchange Membrane Fuel Cells (PEMFC)
Zubiaur, Anthony ULiege; Asset, Tristan ULiege; Olu, Pierre-Yves et al

Poster (2017, September)

Proton exchange membrane fuel cell (PEMFC) catalysts are generally made of carbon black (CB)supported platinum-based nanoparticles. However, the properties of CBs, as described by RodríguezReinoso [1 ... [more ▼]

Proton exchange membrane fuel cell (PEMFC) catalysts are generally made of carbon black (CB)supported platinum-based nanoparticles. However, the properties of CBs, as described by RodríguezReinoso [1], are not optimal for electrocatalysis. A possible solution to the drawbacks of CBs is the use of synthetic nanostructured materials with a controllable and reproducible texture and with a pure, known and constant chemical composition; carbon xerogels (CX) exhibit such properties [2]. Recently, various highly dispersed CX-supported Pt nanoparticles catalysts (Pt/CX) have been synthesized via several methods, such as the strong electrostatic adsorption (SEA) [3] or the charge-enhanced dry impregnation (CEDI) [4]. However, the decrease of the Pt mass contained in the PEMFC electrodes, in particular at the cathode where the oxygen reduction reaction (ORR) is processed, remains a major challenge. Improving the ORR mass activity is currently best achieved by alloying Pt with 3d-transition metal atoms such as cobalt (Co). These alloys perform better than the ORR because the substitution of some Pt atoms by 3d-metal atoms with smaller radius leads to a modified Pt electronic structure [5]. The synthesis and durability of Pt-Co hollow particles/CX (Fig. 1) is currently being studied in our groups. The results indicate that the specific and mass activity of the bimetallic hollow particles dispersed on CX is ca. 10 times higher than that of pure Pt/CB. The first durability results show good stability of the hollow particles structure. Performances and accelerated stress tests (ASTs) in PEMFCs were finally performed. [less ▲]

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See detailCould indolic glucosinolates root profiling be correlated to Brassica napus L. cadmium stress tolerance?
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

Poster (2017, September)

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary ... [more ▼]

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary metabolites hugely produced within Brassicaceae family can interact in regards to cadmium stress as well. Sulfate primary metabolism plays an important role to Brassica napus L. cadmium stress tolerance and glucosinolates could serve as sulfate pool under such stress conditions. We describe here an in vitro experiment performed under sterile and controlled conditions showing the relationship between Brassica napus L. var. Es Astrid tolerance to a low concentration of cadmium and a decrease of a major indolic glucosinolates produced by the roots of this variety. Phenotyping results obtained from these cadmium stress conditions confirm also that Brassica napus L. remains a highly cadmium tolerant species probably due to glucosinolates root metabolism particularly. [less ▲]

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See detailDefeatist performance beliefs in a sample of individuals with persistent negative symptoms
Thonon, Bénédicte ULiege; Li, Xueying; Velligan, Dawn

Poster (2017, September)

Objective Negative symptoms of schizophrenia, in particular motivational deficits, have showed to be linked to dysfunctional attitudes, such as defeatist performance beliefs. This relationship has been ... [more ▼]

Objective Negative symptoms of schizophrenia, in particular motivational deficits, have showed to be linked to dysfunctional attitudes, such as defeatist performance beliefs. This relationship has been found in various types of samples but has not been investigated in samples of individuals presenting persistent negative symptoms (PNS). The present study aimed to assess the relationship between defeatist performance beliefs and negative symptoms in a sample of individuals diagnosed with schizophrenia and PNS. Another objective was to evaluate the link between change in defeatist beliefs and change in negative symptoms, before and after psychosocial intervention. Methods Fifty-one participants diagnosed with schizophrenia and PNS followed 9 months of Motivation and Engagement Training. They were assessed before and after the intervention using the Negative Symptom Assessment (NSA), the Brief Negative Symptom Scale (BNSS), the Clinical Assessment Interview for Negative Symptoms (CAINS), and de the Defeatist Performance Beliefs scale. Results At baseline, defeatist performance beliefs were not significantly associated with negative symptoms, except with the motivation factor of the BNSS, but only at the trend level. However, change in defeatist beliefs was moderately correlated with change in motivation, as measured with the BNSS, but not with the other measures. Conclusion These results underlie the necessity of broadening the targets of psychosocial interventions to factors related to negative symptoms other than dysfunctional attitudes. [less ▲]

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See detailA technological and functional examination of the Aurignacian end-scrapers from Grotta di Fumane
Aleo, Alessandro; Duches, Rossella; Rots, Veerle ULiege et al

Poster (2017, September)

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See detailDietary inulin supplementation promotes weight loss in obese individuals
Hiel, Sophie; Rodriguez, Julie; Gianfrancesco, Marco ULiege et al

Poster (2017, September)

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See detailNew filamentous mutant of Yarrowia lipolytica and its use in biofilm bioreactors
Vandermies, Marie ULiege; Kar, Tambi ULiege; Denies, Olivia ULiege et al

Poster (2017, September)

The non-conventional yeast Yarrowia lipolytica is widely investigated for its unusual metabolic properties. Among them is the ability of Y. lipolytica to adopt an ovoid or hyphal morphology according to ... [more ▼]

The non-conventional yeast Yarrowia lipolytica is widely investigated for its unusual metabolic properties. Among them is the ability of Y. lipolytica to adopt an ovoid or hyphal morphology according to environmental conditions. The mechanism of dimorphic transition involves numerous genes, which have been poorly documented to date. Here, we report on the isolation of a filamentous mutant from an insertion mutagenesis library, the subsequent identification of the mutated gene, and the use of this filamentous mutant in biofilm bioreactors. [less ▲]

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See detailUseful tools for genome editing in the non-conventional yeast Yarrowia lipolytica
Vandermies, Marie ULiege; Denies, Olivia ULiege; Nicaud, Jean-Marc et al

Poster (2017, September)

The non-conventional yeast Yarrowia lipolytica is increasingly used for the production of recombinant proteins or biomolecules with biotechnological or pharmaceutical applications. The development of such ... [more ▼]

The non-conventional yeast Yarrowia lipolytica is increasingly used for the production of recombinant proteins or biomolecules with biotechnological or pharmaceutical applications. The development of such a cell factory requires steps of genome editing that rely on selectable markers. The recently identified EYK1, encoding erythrulose kinase, can serve as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Besides, the cloning-free strategy developed here simplifies the construction of disruption cassettes for genome editing in Y. lipolytica. [less ▲]

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See detailPromoting "health literacy friendly" healthcare institutions
Henrard, Gilles ULiege

Poster (2017, September)

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See detailRecycling of silicon used in solar cells to prepare silicon-carbon composite powders as anode material in li-ion batteries
Eshraghi, Nicolas ULiege; Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege et al

Poster (2017, September)

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as ... [more ▼]

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as an electrode material for lithium or sodium batteries. The main technological challenge associated with the use of silicon in this type of application is to control the volume expansion during charge/discharge cycles. This problem could be solved through the synthesis of Silicon/carbon composites in which the size of the silicon particles and their dispersion must be controlled [1–4]. We develop a carbon matrix consisting of graphene or carbon nanotubes (CNT) that allow the incorporation of silicon particles coated with a carbon layer (Si@C/C). The process is divided in two main steps. In the first step, Si wafer pieces are ground to prepare Si powder and then a mixed suspension of Si and an organic carbon source (Acetic acid, Ascorbic acid or Lactose) is spray-dried followed by heat treatment to generate the coverage of silicon particles with carbon (Si@C). In the second step, aqueous suspension of Si@C and graphene/CNT is spray dried and heat treated to obtain the final composite structure. The morphology of composite materials is analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Electrochemical performance of Si@C/C composites are characterized by galvanostatic charge-discharge cycling and electrochemical impedance spectroscopy (EIS). [less ▲]

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See detailPreparation and characterization of Silicon-carbon composite powders using recycled Silicon from solar cells as anode material in Li-ion batteries
Eshraghi, Nicolas ULiege; Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege et al

Poster (2017, September)

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as ... [more ▼]

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as electrode material for Li-ion batteries. The main technological challenge associated with the use of silicon in this type of application is to control the volume expansion during charge/discharge cycles. This drawback could be avoided through the formation of Silicon/carbon composites in which the size of the silicon particles and their dispersion must be controlled. In this research, we develop a carbon matrix consisting of graphene or carbon nanotubes (CNT) that allow the incorporation of silicon particles coated with a carbon layer (Si@C/C). The process is divided in two main steps : I) the grinding of leached Si wafer pieces in order to extract pure Si powder and then a mixed aqueous suspension of this recycled Si and an organic carbon source (Acetic acid, Ascorbic acid or Lactose) is spray-dried followed by heat treatment to generate the coating of silicon particles with carbon (Si@C). Then, II) aqueous suspension of Si@C and graphene/CNT is spray dried and calcined to obtain the final composite structure (Si@C/C). The morphology of composite materials is analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Electrochemical performance of Si@C/C composites are characterized by galvanostatic charge-discharge cycling and electrochemical impedance spectroscopy (EIS). [less ▲]

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See detailFlowering roots: Insensitive Root Growth 1 contributes to photoperiod-induced root responses in Arabidopsis.
Orman-Ligeza, Beata ULiege; Detry, Nathalie ULiege; Tocquin, Pierre ULiege et al

Poster (2017, September)

The capacity to perceive and respond to seasonal changes of day length is essential for flowering plants. Under favourable photoperiod, a mobile stimulus synthesized in leaves moves to the shoot apex and ... [more ▼]

The capacity to perceive and respond to seasonal changes of day length is essential for flowering plants. Under favourable photoperiod, a mobile stimulus synthesized in leaves moves to the shoot apex and triggers the expression of genes required for the transition to flower initiation. Although transition from vegetative to reproductive state also encompasses a transcriptional response in roots, the internal signalling pathways and how root system architecture adjusts to this changing status remain elusive. Here we show in Arabidopsis that root growth rate increases upon a transfer to flowering-inductive long days while remaining constant under short days. To elucidate genetic components of this response, we performed a meta-analysis of available root-growth and flowering-related arrays and selected genes with overlapping transcriptional profiles for further analyses. Loss of function in a member of the basic leucine zipper transcription factor gene family, hereafter named Insensitive Root Growth-1 (IRG1), was found to suppress photoperiod-response of root growth with no defect in flowering time. We show that sucrose, but neither glucose nor mannitol in the growth medium under long days, is needed to trigger this response. In addition, extending the photoperiod with non-photosynthetic far red light had no effect on root growth of irg-1 mutant, alike wild type Col-0. The expression level of IRG1 in the roots remains low during the daytime and peaks late at night, suggesting that this gene is regulated by the clock’s evening loop. Taken together, our results suggest that IRG1 may be involved in sucrose-mediated stimulation of root growth during the night phase in Arabidopsis. The functional characterisation of IRG1 is currently underway. [less ▲]

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See detailTowards understanding the function of JOINTLESS gene in tomato inflorescence
Huerga Fernandez, Samuel ULiege; Gómez Roldán, Maria Victoria; Orman-Ligeza, Beata ULiege et al

Poster (2017, September)

The lack of fruit abscission is a trait of great agronomical value. In tomato, the jointless phenotype, referring to the lack of abscission zone (AZ) in the flower pedicel, has been obtained by two ... [more ▼]

The lack of fruit abscission is a trait of great agronomical value. In tomato, the jointless phenotype, referring to the lack of abscission zone (AZ) in the flower pedicel, has been obtained by two independent mutations, named jointless (j) and jointless-2 (j-2). The corresponding genes encode MADS-box transcription factors, as shown in 2000 for J (Mao et al. 2000) and very recently for J-2, known as SlMBP21 (Gomez-Roldan et al., 2017). Similar to the quartet model of MADS-box protein complexes regulating floral organ formation, J and J-2 interact with MADS-box partners, among which MACROCRALYX (MC), to regulate AZ formation (Liu et al. 2014). In addition to - or in connection with - AZ formation, J acts during the building of the inflorescence to regulate meristem fate. Indeed j mutants produce leafy inflorescences characterized by faster flower maturation and resumption of vegetative meristems (Périlleux et al. 2014). For these traits, j is epistatic to j-2. The involvement of J in the regulation of meristem fate is consistent with the roles of its closest homologs in Arabidopsis, AGAMOUS LIKE 24 (AGL24) and SHORT VEGETATIVE PHASE (SVP). Our goal is to identify J targets in order to unravel its multiple functions in the tomato inflorescence. [less ▲]

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See detailVINYLIC COPOLYMERS FOR PH-SENSITIVE LIPID NANOCAPSULES
Pautu, Vincent ULiege; Lepeltier, Elise; Debuigne, Antoine ULiege et al

Poster (2017, September)

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See detailUp-scalable spray-drying synthesis of Na2Ti3O7
Piffet, Caroline ULiege; Vertruyen, Bénédicte ULiege; Mahmoud, Abdelfattah ULiege et al

Poster (2017, September)

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See detailTargeted mutagenesis of orphan GPCRs of the SREB family
Laschet, Céline ULiege; Dupuis, Nadine ULiege; Geubelle, Pierre ULiege et al

Poster (2017, September)

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See detailLexical Diachronic Semantic Maps
Georgakopoulos, Athanasios ULiege; Polis, Stéphane ULiege

Poster (2017, September)

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See detailHeterotrophy compared to phototrophy for growth characteristics and pigment compositions in batch cultures of four green microalga
Le Thanh Tung, ULiege; Corato, Amélie ULiege; Gerards, Thomas ULiege et al

Poster (2017, September)

Growth curves of four species of green microalgae have been compared under photoautotrophy, heterotrophy and dim-light-assisted heterotrophy during batch cultivation in small-scale multicultivators ... [more ▼]

Growth curves of four species of green microalgae have been compared under photoautotrophy, heterotrophy and dim-light-assisted heterotrophy during batch cultivation in small-scale multicultivators equipped with automated optical density acquisition. The results showed that the most relevant biomass productivity parameter (based on Vmax) can be estimated after proper correction of growth curves. For photoautotrophic cultures, Vmax showed no clear relationship to µmax, the specific growth rate measured during exponential phase. Heterotrophic cultures, which are not limited by light penetration, can be characterized by their µmax values, which showed a broad range of values depending more on species than on carbon source (glucose or acetate). Weak light (defined as unable to sustain photoautotrohic growth) had only minor effects on heterotrophic growth in terms of biomass but, for some species, could be used to promote pigment accumulation up to levels comparable to those found under photoautotrophy, as revealed by HPLC analysis. [less ▲]

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See detailCharacterisation of a secondary carotenoid producer microalga of the genus Coelastrella
Corato, Amélie ULiege; Franck, Fabrice ULiege; Jacques, Philippe ULiege

Poster (2017, September)

Some green microalgae synthesize secondary carotenoids as protecting agents under stress. These pigments have high value as feed supplement for aquaculture and as health products. The most promising ... [more ▼]

Some green microalgae synthesize secondary carotenoids as protecting agents under stress. These pigments have high value as feed supplement for aquaculture and as health products. The most promising pigment is astaxanthin, because of its antioxidant, antitumoral and anti-inflamatory properties. The most used natural source of this pigment is the microalga Haematococcus pluvialis. However this species grows slowly and lacks robustness for easy cultivation. Therefore, other species are investigated for astaxanthin production. Here, we identified a locally isolated strain as Coelastrella sp. that is a secondary carotenoid producer. [1] A known typical feature of this genus, that we could observed in the strain by scanning electron microcopy, is the presence of meridional ribs. [2] We analyzed the culture conditions and concluded that this strain grows both autotrophically and heterotrophically and is able of fast change in pigment composition under controlled stress conditions. Thanks to HPLC analyses, we determined that the strain accumulates a variety of secondary carotenoids, among which: astaxanthin, cantaxanthin and echinenone. Unidentified compounds will be further analyzed by mass spectrometry. [less ▲]

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See detailBio-chemical Raman imaging using targeted-SERS
Verdin, Alexandre ULiege; Malherbe, Cédric ULiege; Eppe, Gauthier ULiege et al

Poster (2017, September)

Raman and SERS spectral imaging, which consists in reconstructing a Raman intensity profile over a 2D or 3D section of a given sample (in presence or not of a nanosubstrate), allow to visualise the ... [more ▼]

Raman and SERS spectral imaging, which consists in reconstructing a Raman intensity profile over a 2D or 3D section of a given sample (in presence or not of a nanosubstrate), allow to visualise the variation of the chemical composition associated with the micro-structure of the sample. In particular, targeted-SERS imaging also enables the specific imaging of various macromolecules targeted by SERS probes in complex biological samples such as cancerous cells. To illustrate the versatility of Raman imaging, we describe two applications that were investigated recently in our laboratory. First, 3D Raman imaging was used to investigate a microcavity in a matrix of gypsum, revealing fossilised carbonaceous compounds dispersed in a filamentous shape, highly suggesting the presence of fossilised bacteria. Secondly, SERS probes were synthesised to specifically target the FRα receptor of folic acid which are often over-expressed at the surface of cancerous cells. Confocal Raman imaging was used to study the localization of the probes after incubation with KB cells (oral cancer) to confirm the specific targeting of FRα membrane receptors (Fig. 1). Moreover, our SERS probes allowed us to distinguish two different cancerous cell lines (namely KB and PC-3), based on the degree of expression of the FRα receptor of these two lines (high for KB and low for PC-3). In the future, these SERS probes may be used for distinguishing between cancerous and healthy cells since healthy cells have a lower degree of expression of FRα. [less ▲]

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See detailIL-4Ra-dependent macrophage responses in the liver during murine schistosomiasis
Rolot, Marion ULiege; Dougall, Annette; Gillet, Laurent et al

Poster (2017, September)

During Schistosoma mansoni infection in mice, IL-4 receptor-dependent alternatively activated macrophages (aaMφ) response mediate host protection. Here, we used LysMcreIl4ra−/lox mice to determine whether ... [more ▼]

During Schistosoma mansoni infection in mice, IL-4 receptor-dependent alternatively activated macrophages (aaMφ) response mediate host protection. Here, we used LysMcreIl4ra−/lox mice to determine whether the absence of Mφ-specific IL-4 receptor signalling modifies the dynamics of Mφ responses in liver granulomas after S. mansoni infection. Liver inflammatory response was investigated after low-dose S. mansoni infection. We observed significantly increased total leukocyte numbers in the liver by week 8 in LysMcreIl4ra−/lox mice while mice survived the infection similarly to littermate controls. Over the course of infection, we observed that CD11blowMerTK+CD64+ resident Küpffer cells (KCs) were severely reducing in term of numbers independently of IL-4Rα signalling. While KCs lowered, Ly6Chi monocytes were recruited in the liver from 6 weeks pi, proliferate strongly at week 8 and acquire CD64 expression. The number of Ly6Chi recruited monocytes were significantly higher in LysMcreIl4ra−/lox mice than littermate controls. From 8 weeks pi, CD11blow KCs seemed to be replaced by CD11bhighMerTK+CD64+F4/80+ Mφ, probably derived from recruited Ly6Chi monocytes. At week 8 pi, expression of Ym1 and Relm-alpha was significantly reduced in hepatic CD11bhigh Mφ of LysMcreIl4ra−/lox mice compared to littermate controls. These results suggest that recruited monocytes differentiate into Mφ at the cost of resident KCs independently of IL-4Rα and validate the use of LysMcreIl4ra−/lox mice to study IL-4Rα-dependent activation of monocyte-derived granuloma Mφ during schistosomiasis. [less ▲]

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See detailAn active bacterial community linked to high chl-a concentrations in Antarctic winter-pack ice and evidence for the development of an anaerobic sea-ice bacterial community
Eronen-Rasimus; Luhtanen, A.-M.; Rintala, J.-M. et al

Poster (2017, September)

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See detailSynchronous fluorescence spectroscopy for detecting blood meal and blood products in feed
Abbas, Ouissam; Lecrenier, Marie-Caroline ULiege; Baeten, Vincent

Poster (2017, September)

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See detailThe acoustic correlates of hypnotic voice
Remacle, Angélique ULiege; Todovora, Tatiana; Zambra, Nurihan

Poster (2017, August 31)

Although the linguistic content of hypnotic suggestions has been investigated in various studies, the paralinguistic aspects – that is, the voice used by hypnotherapists – are little described. The few ... [more ▼]

Although the linguistic content of hypnotic suggestions has been investigated in various studies, the paralinguistic aspects – that is, the voice used by hypnotherapists – are little described. The few indications concerning vocal patterns that promote a state of hypnotic trance are limited to subjective descriptions based on clinical observations, as reported in textbooks (Grinder & Bandler, 1997; James, 2010). From an empirical point of view, voice characteristics have been assessed objectively with acoustic measures in the context of muscle relaxation (Knowlton & Larkin, 2006); however, to the best of our knowledge, such measures have not been applied to identify vocal indices of the voice used to induce hypnosis. The aim of this study is to investigate the existence of a voice pattern specific to hypnosis. To that end, 32 French-speaking therapists (18 women and 14 men) who practice hypnosis in a medical or psychological context were recorded while reading a single text in two conditions: with a normal voice and with a voice used for hypnotic induction. For each of these two conditions, the following acoustic analyses were conducted with Praat software: degree of voicing (in %), mean fundamental frequency (F0, in Hz), mean sound pressure level (SPL, in dB), and variability of F0 and SPL. Statistical analyses (Wilcoxon test) show that, compared to a normal voice, a hypnotic voice presents less voicing (p<.001), lower F0 and SPL (p<.001), less SPL variability (p=.003), and a trend toward less F0 variability (p=.056). These preliminary results allow us to provide objective acoustic correlates of a hypnotic voice, such as low frequency, low intensity, monotone and lack of voicing. These acoustic characteristics correspond to the subjective voice descriptions given in textbooks, such as quiet, gentle, reassuring, and warm, which tend to create a sense of intimacy and confidentiality. Regarding the lower degree of voicing in hypnotic voices, subsequent analyses will make it possible to test the hypothesis that the voice is breathy and whispery, at least at times, and that there are longer pauses than in a normal voice. Analyses of spontaneous speech during hypnosis sessions are currently under way to test the generalizability of the results presented here. References -Grinder, J., & Bandler, R. (1997). Patterns of the hypnotic techniques of Milton H. Erickson, M.D. Vol. I. Portland, OR: Metamorphous Press. -James, U. (2010). Clinical hypnosis textbook: A guide for practical intervention (2nd ed.). Oxford, UK, New York: Radcliffe Publishing Ltd. -Knowlton, G. E., & Larkin, K. T. (2006). The influence of voice volume, pitch, and speech rate on progressive relaxation training: Application of methods from speech pathology and audiology. Applied Psychophysiology and Biofeedback, 31(2), 173–185. doi:10.1007/s10484-006-9014-6 [less ▲]

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See detailFace description abilities predict line-up performance
Vanootighem, Valentine ULiege; Brédart, Serge ULiege; Dehon, Hedwige ULiege

Poster (2017, August 31)

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See detailThe Economic Development Pole (EDP) approach in Niger: the case of the Niger-IFAD Program
Andres, Ludovic ULiege; Populin, Martha; Bodé, Sambo et al

Poster (2017, August 29)

Since 2012, the Niger-IFAD Programme uses a new non-administrative geographical intervention unit, the Economic Development Pole (EDP). The EDP is described as a space concentrating economic activities ... [more ▼]

Since 2012, the Niger-IFAD Programme uses a new non-administrative geographical intervention unit, the Economic Development Pole (EDP). The EDP is described as a space concentrating economic activities linked to the production and commercialization of the main agro-pastoral products of the neighbouring production basins. This poster aims to describe and analyze the concept of EDP, to show its first results when put into practice and to analyze its risks. [less ▲]

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See detailMultiple Uses of a 3D Point Cloud: the Castle of Franchimont (Province of Liège, Belgium)
Luczfalvy Jancsó, Andrea ULiege; Jonlet, Benoît ULiege; Hallot, Pierre ULiege et al

Poster (2017, August 29)

This poster presents the identified obstacles, needs and selected solution for the study of the medieval castle of Franchimont, located in the province of Liège (Belgium). After taking into account the ... [more ▼]

This poster presents the identified obstacles, needs and selected solution for the study of the medieval castle of Franchimont, located in the province of Liège (Belgium). After taking into account the requirements from all the disciplines at work as well as the problems that would have to be tackled, the creation of a 3D point cloud was decided. This solution would be able to deal with the characteristics and needs of a research involving building archaeology and related fields. The decision was made in order to manage all of the available data and to provide a common working tool for every involved cultural heritage actor. [less ▲]

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See detailFragmentation index of DNA sperm in llama semen (Lama glama)
Cucho, Hernan; Ordonez, Cesar; Ampuero, Enrique et al

Poster (2017, August 25)

DNA fragmentation of spermatozoa is an important indicator of seminal quality and fertility. The quality of the genetic information contained in the DNA molecule transmitted by the spermatozoa is ... [more ▼]

DNA fragmentation of spermatozoa is an important indicator of seminal quality and fertility. The quality of the genetic information contained in the DNA molecule transmitted by the spermatozoa is important for the proper development of the embryo. In humans it has been shown that there is a significant correlation between sperm DNA damage and oocyte fertilization, embryo quality, development to blastocyst and embryo implantation. The objective of this study was to analyze the fragmentation of DNA sperm in llama semen collected by post-copulation vaginal aspiration. The collection of 4 llamas was performed on three occasions per animal. The volume, motility, concentration, vitality, sperm membrane functionality assessed with the Integrated Semen Analysis System - ISAS®. The DNA fragmentation index was tested with the Halomax system ®(Halotech company). Volume, motility, vitality and sperm concentration respectively were 1.3 ± 0.8 ml, 12.30 ± 2.02%; 64.69 ± 11.39% and 183.55 ± 28.48 x 106 spz/ml. The functionality of the sperm membrane was 64.95 ± 11.63% and sperm DNA fragmentation index 11.58 ± 13.25%. The values of these different parameters indicate the high quality of the collected semen. [less ▲]

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See detailCompositional gap at La Picada (CSVZ, Chile) results from critical cristallinity and compaction
Vander Auwera, Jacqueline ULiege; Namur, Olivier ULiege; Coumont, Valentin et al

Poster (2017, August 16)

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See detailMagma storage conditions and processes at Calbuco volcano (Central Southern Volcanic Zone, Chile)
Montalbano, Salvatrice ULiege; Namur, Olivier ULiege; Schiano, Pierre et al

Poster (2017, August 16)

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See detailTailoring the superconducting properties of niobium nanoconstrictions via electro-annealing
Lombardo, Joseph ULiege; Baumans, Xavier ULiege; Jelic, Zeljko ULiege et al

Poster (2017, August 10)

In this work we report the possibility to create weak links in nanostructured Nb thin films using electro-annealing. By using electron-assisted atom diffusion in Nb nanconstrictions, we were able to ... [more ▼]

In this work we report the possibility to create weak links in nanostructured Nb thin films using electro-annealing. By using electron-assisted atom diffusion in Nb nanconstrictions, we were able to locally change the material properties (superconducting critical temperature and normal resistance). When the critical temperature of the nanoconstriction is sufficiently decreased, the field dependence of the critical current exhibits a Fraunhofer-like pattern, confirming the formation of the weak link. Scanning electron microscopy images clearly reveals the structural change underwent by the constriction resulting from the effect of the high current density. Numerical simulations within the Ginzburg-Landau formalism are also presented. These findings represent the first evidence of weak link formation by electro-annealing and provide an easy method for the fabrication of Josephson junctions with tunable resistance and critical temperature in Nb thin films. [less ▲]

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See detailA novel approach towards manipulation of vortex matter in a superconductor with micromagnetic structures
Shaw, Gorky ULiege

Poster (2017, August 10)

Hard permanent magnetic materials offer a number of advantages over softer magnetic materials as sources of vortex pinning in a superconductor, like greater stability and larger magnetic field amplitudes ... [more ▼]

Hard permanent magnetic materials offer a number of advantages over softer magnetic materials as sources of vortex pinning in a superconductor, like greater stability and larger magnetic field amplitudes. Permanent micromagnet structures prepared using thermomagnetic patterning (TMP) present an interesting and so far unexplored option for controlled artificial pinning. We have investigated the vortex matter in superconductor/TMP micromagnet heterostructures (Nb-NdFeB) using quantitative Magneto-Optical Imaging (MOI). Comprehensive protocols have been developed for calibrating and converting Faraday rotation data acquired by MOI to magnetic field maps. These protocols reveal the comparatively weaker magnetic response of the superconductor from the background of larger fields associated with the magnetic layer in its vicinity. Further, TMP micromagnet structures have been imprinted in a Permalloy (Py) layer to obtain flexible magnetic landscapes for flux guidance in a Nb layer below it. Both smooth flux penetration and vortex avalanches in Nb are observed to be strongly influenced by the micromagnetic patterns. Our study offers new insights into the peculiarities of the vortex state in these superconductor-micromagnet heterostructures. [less ▲]

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See detailThe role of the tegument protein ORF9p and its phosphorylation status in VZV nuclear egress
Lebrun, Marielle ULiege; Blondeau, Caroline; Riva, Laura et al

Poster (2017, August 02)

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See detailAssessing quality of life using structural equation modeling
Dardenne, Nadia ULiege; Pétré, Benoît ULiege; Husson, Eddy ULiege et al

Poster (2017, August)

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See detailPrevalence of voice disorders among teachers at the beginning of their career
Schiller, Isabel ULiege

Poster (2017, August)

Due to the high vocal demands associated with their profession, teachers face an increased risk of developing voice disorders. Research suggests that up to 50 % of experienced teachers are affected. Even ... [more ▼]

Due to the high vocal demands associated with their profession, teachers face an increased risk of developing voice disorders. Research suggests that up to 50 % of experienced teachers are affected. Even student teachers, whose vocal load is still relatively low, report voice problems with a frequency of 20 % (Simberg, Laine, Sala, & Rönnemaa, 2000). Little is known about the prevalence of voice disorders among teachers at the very beginning of their career. In Germany, teachers must complete a two-year teaching practice as part of their professional training after they graduate from university, consisting of attending specialized courses and giving lessons. The aim of this symptom-based study was to determine the prevalence of voice problems among this population. A self-administered questionnaire was answered by 73 German teachers in teaching practice. The prevalence of a voice problem was defined based on the presence of two symptoms that persisted for a minimum of two weeks. Results revealed that 37 % of teachers had voice problems during their teaching practice. Among the most frequent symptoms were hoarseness, an urge to clear one’s throat and problems speaking at a loud voice. Considering that the participants had not even begun teaching full-time, the prevalence of reported voice problems is worrisome. It could be associated with their lacking experience in how to use their voice effectively. The findings highlight the necessity of early intervention programs focusing on vocal hygiene and effective voice use in classroom situations. [less ▲]

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See detailEvaluation of two intravaginal implants for the synchronization of estrus in Criollo cattle in Peru
Ordonez, Cesar; Ampuero, Enrique; Huanca, Nancy et al

Poster (2017, August)

The objective of this study was to compare the effect of two vaginal implants protocols on the follicular characteristics, the vaginal electrical resistance and the pregnancy rate of criollo cattle reared ... [more ▼]

The objective of this study was to compare the effect of two vaginal implants protocols on the follicular characteristics, the vaginal electrical resistance and the pregnancy rate of criollo cattle reared in altitude (3100 meters). All the cows (n=53) were selected after manual palpation and ultrasound examinations of their genital tract. All the cows had a body condition between 2.5 and 3.5 (range 1 to 5). On day 1 et for 7 days the first group of cows (n=23) was treated with 1,38 g of progesterone (Easi-breedTM CIDR® Zoetis) and the second group (n=30) with 1 g of progesterone (DIB system Agrihealth New Zealand). All the cows were treated at day 1 with 2 mg of estradiol benzoate (im), at day 9 with 2 ml of prostaglandin (im) and at day 10 with 1 mg of estradiol benzoate. A timed artificial insemination was realized 54 hours after the CIDR® or DIB removal. The pregnancy diagnosis was made by ultrasound 45 days later. Vaginal conductivity, diameter and perimeter of the follicle were measured at the time of insemination. There were no significant differences (p> 0.05) between both treatments in the electrical resistance of the vaginal mucus, diameter and perimeter of the preovulatory follicule. The averages of these variables respectively were 233 ± 36 ohms, 9.1 ± 1.9 mm and 24.6 ± 4.7 mm. Pregnancy rates of cows treated with DIB and CIDR respectively were 63.3 % and 69.6%. No significant statistical differences (p> 0.05) have been seen. In conclusion, both treatments showed similar ovarian response, electrical resistance and pregnancy rate in criollo cattle reared in altitude. [less ▲]

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See detailLe vocabulaire des archives des cités grecques : l’apport de l’épigraphie. Le cas des termes ἀποδέκτης et ἀποδοχεύς
Dolne, Laëtitia ULiege

Poster (2017, August)

Ce poster est issu d’un projet de thèse récemment débuté à l’Université de Liège et consacré à l’étude du vocabulaire technique des différents aspects de l’archivage dans les cités grecques antiques. Un ... [more ▼]

Ce poster est issu d’un projet de thèse récemment débuté à l’Université de Liège et consacré à l’étude du vocabulaire technique des différents aspects de l’archivage dans les cités grecques antiques. Un des objectifs du projet est de réaliser un lexique systématique, exhaustif et raisonné, fondé sur une analyse approfondie et contextualisée des sources littéraires, épigraphiques et papyrologiques grecques et latines de c. IIIe s. av. J.-C. à c. IIIe s. apr. J.-C. Une fois achevé, le lexique facilitera pour tous l’accès aux sources de l’activité archivistique ancienne et, de ce fait, sa compréhension et son étude dans un cadre plus global. En effet, la lecture de ces sources est entravée par la diversité et la technicité des termes qui y abondent et peuvent avoir, selon le contexte, mais aussi le lieu et l’époque, des acceptions différentes, et donc désigner des réalités distinctes. Le poster illustre cette problématique, ainsi que la méthodologie employée dans le projet, par l’exemple des termes ἀποδέκτης et ἀποδοχεύς. Alimenté principalement par la documentation épigraphique, le cas de figure choisi montre également l’important apport de cette discipline pour la réalisation d’un tel lexique. [less ▲]

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See detailCharacterising Industrial Sites' Flexibility with Reservoir Models
Cuvelier, Thibaut ULiege

Poster (2017, August)

Electro-intensive industrial sites are very dependent on electricity prices to remain competitive. Nevertheless, they can often tune their processes in order to decrease their electricity consumption ... [more ▼]

Electro-intensive industrial sites are very dependent on electricity prices to remain competitive. Nevertheless, they can often tune their processes in order to decrease their electricity consumption during the most critical periods, for example by using decision support systems based on mathematical modelling of their processes. Our goal is to estimate the flexibility potential of a complete site, not to tune each process very precisely. To this end, we propose a generic paradigm to help conceiving such models: reservoirs are the basic building block, which allows for great expressiveness while being close to the physics. More specifically, we do not need very precise models for our purposes, but ones that can be efficiently included in optimisation models. Our first results show that the obtained reservoir models can give sufficiently good approximations for metallurgical and other processes. [less ▲]

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See detailDesign and Synthesis of [18F]BPAM121, a PET-Probe Targeting AMPA-subtype Glutamatergic Receptors.
Manos-Turvey, Alexandra ULiege; Lemaire, Christian ULiege; Becker, Guillaume ULiege et al

Poster (2017, August)

AMPA receptors (AMPARs), one of three sub-groups of ionotropic glutamate receptors present in the central nervous system, are recognised for their involvement in long-term potentiation (LTP), and learning ... [more ▼]

AMPA receptors (AMPARs), one of three sub-groups of ionotropic glutamate receptors present in the central nervous system, are recognised for their involvement in long-term potentiation (LTP), and learning and memory processes. [1] They represent a valid cognitive enhancer target, particularly in the fight against Alzheimer’s disease (AD). [2,3] Benzothiadiazine 1,1-dioxides, such as BPAM121, have emerged as important allosteric modulators of AMPARs, working solely in the presence of the endogenous transmitter. [4] Synthesis of BPAM121 labelled with fluorine-18 was proposed, to investigate the utility of this molecule as a PET probe in vivo, and evaluate its potential as an AD diagnostic tool (Figure 1). [Figure 1. a) Structure of BPAM121, b) Established radiochemical synthesis of [18F]BPAM121.] This work documents the successful optimization of synthesis, purification and formulation of [18F]BPAM121 using an automated FASTlab (GE Healthcare) synthesizer. In particular, the influence of higher-level [18F]fluoride ion starting concentrations on final product formulation requirements is discussed. Initial results revealed [18F]BPAM121 successfully passes the blood brain barrier, and further biological studies are currently underway. References [1] S. F. Traynelis et al. Pharmacol. Rev. 2010, 62, 405-496. 
 [2] J. Keifer, Z. Zheng, Eur. J. Neurosci. 2010, 32, 269-277. 
 [3] L. Gao et al. J. Neurochem. 2016, 136, 620-636. 
 [4] P. Francotte et al. J. Med. Chem. 2010, 53, 1700-1711. 
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See detailSpatial and temporal variability of microbial soil quality indicators in two forest catchments
Carnol, Monique ULiege; Bosman, Bernard ULiege; Ratcliffe, Sophia ULiege

Poster (2017, August)

Appropriate indicators for assessing forest soil quality are needed to monitor changes in the frame of air pollution and climate change, assess impacts of forest management actions and consequently decide ... [more ▼]

Appropriate indicators for assessing forest soil quality are needed to monitor changes in the frame of air pollution and climate change, assess impacts of forest management actions and consequently decide on long-term management objectives. In addition to chemical indicators, such as for example soil organic carbon and pH, biological indicators inform on soil functions/processes and provide an early diagnosis of changes in the environment. The objectives of this work were to provide first baseline values for Belgian forest soils, to evaluate the sensitivity of selected indicators to changes in tree species on the same soil type, and to assess the temporal variability of the indicators. This work is part of a long-term study on element concentrations and fluxes in 2 small (each ca. 80 ha) forested watersheds (Waroneu and Robinette, east Belgium) in relation to forest management. Both catchments show spatial variability of tree species cover and drainage. In particular, Robinette was partially clear-cut in 1996 and replanted with deciduous tree species in 1998. Six sub-plots with a specific tree species/soil hydrology combination were defined in each catchment. Selected biological indicators (potential respiration, nitrogen mineralization, microbial biomass, metabolic diversity of bacteria) and two ecophysiological indices (microbial quotient and metabolic quotient) were measured in spring and autumn during 3 consecutive years. Our results suggest that (1) radar plots can be a useful tool to visualize the ranges of several indicators simultaneously (2) the biological indicators showed significant differences between the sub-plots, even though they are situated on the same geological substrate and in a limited geographical region, indicating high sensitivity to detect changes (3) Small scale differences detected raise the question of the appropriate spatial scale and classifying criteria to define baseline values (4) there were no marked seasonal differences, but for some indicators (N mineralization, potential respiration, microbial and metabolic quotients) the sampling moment induced a certain variability. [less ▲]

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See detailThermoelectric properties of heavily-doped Fe2YZ full-Heusler compounds
Lemal, Sébastien ULiege; Ricci, Fabio ULiege; Verstraete, Matthieu ULiege et al

Poster (2017, July 31)

Fe2YZ full-Heusler compounds were recently predicted to exhibit very large thermoelectric power factors [Phys. Rev. Lett. 114, 136601 (2015)]. Combining first-principles density functional theory ... [more ▼]

Fe2YZ full-Heusler compounds were recently predicted to exhibit very large thermoelectric power factors [Phys. Rev. Lett. 114, 136601 (2015)]. Combining first-principles density functional theory calculations within a hybrid functional approach and Boltzmann semi-classical transport theory, we investigate the limits of the rigid band doping approximation, and the properties of heavily n-type doped full-Heusler Fe2YZ1-xAx compounds. Using a supercell approach and including explicit dopant impurities, we recover in some cases giant thermoelectric power factors, as previously predicted within the rigid band approximation. In others the effect on the electronic structure is very different from rigid shifts. Interestingly, we find that some systems present a magnetic instability consistent with the Stoner model, and evolve towards a ferromagnetic half-metallic ground state, with a strongly modified power factor. Our results show the promise of the heavily-doped phases of the studied Fe2YZ1-xAx compounds for Seebeck and spin-dependent Seebeck applications. [less ▲]

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See detailThe Prediction-Focused Approach: An opportunity for hydrogeophysical data integration and interpretation in the critical zone
Hermans, Thomas ULiege; Nguyen, Frédéric ULiege; Klepikova, Maria et al

Poster (2017, July 27)

Two important challenges remain in hydrogeophysics: the inversion of geophysical data and their integration in quantitative subsurface models. Classical regularized inversion approaches suffer from ... [more ▼]

Two important challenges remain in hydrogeophysics: the inversion of geophysical data and their integration in quantitative subsurface models. Classical regularized inversion approaches suffer from spatially varying resolution and yield geologically unrealistic solutions, making their utilization for model calibration less consistent. Advanced techniques such as coupled inversion allow for a direct integration of geophysical data; but, they are difficult to apply in complex cases and remain computationally demanding to estimate uncertainty. We investigated a prediction-focused approach (PFA) to directly estimate subsurface physical properties relevant in the critical zone from geophysical data, circumventing the need for classic inversions. In PFA, we seek a direct relationship between the data and the subsurface variables we want to predict (the forecast). This relationship is obtained through a prior set of subsurface models for which both data and forecast are computed. A direct relationship can often be derived through dimension reduction techniques (Figure 1). For hydrogeophysical inversion, the considered forecast variable is the subsurface variable, such as the salinity or saturation for example. An ensemble of possible solutions is generated, allowing uncertainty quantification. For data integration, the forecast variable is the prediction we want to make with our subsurface models, such as the concentration of contaminant in a drinking water production well. Geophysical and hydrological data are combined to derive a direct relationship between data and forecast. We illustrate the methodology to predict the energy recovered in an ATES system considering the uncertainty related to spatial heterogeneity. With a global sensitivity analysis, we identify sensitive parameters for heat storage prediction and validate the use of a short term heat tracing experiment to generate informative data. We illustrate how PFA can be used to successfully derive the distribution of temperature in the aquifer from ERT during the heat tracing experiment. Then, we successfully integrate the geophysical data to predict heat storage in the aquifer using PFA. The result is a full quantification of the posterior distribution of the prediction conditioned to observed data in a relatively limited time budget. [less ▲]

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See detailBuilding flow and transport models with electrical resistivity tomography data
Gottschalk, Ian; Hermans, Thomas ULiege; Knight, Rosemary et al

Poster (2017, July 26)

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of ... [more ▼]

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of lithological heterogeneity at ARR sites often hinders attempts to predict where and how quickly infiltrating water will flow in the subsurface, which can adversely affect the quality and quantity of available water in the ARR site. In this study, we explored the use of electrical resistivity tomography (ERT) to assist in characterizing lithological heterogeneity at an ARR site, so as to incorporate it into a flow and contaminant transport model. In this case, we had non-collocated well core log data and ERT data from a full-scale ARR basin. We compared three independent methods for producing conditional lithology-resistivity probability distributions: 1) a search template to relate the nearest logged well lithologies with ERT resistivity panels, given search criteria; 2) a maximum likelihood estimation (MLE) to match bimodal normal distributions to the histogram of each ERT line; and 3) variogram-based lithology indicator simulations constrained to well data. Each approach leverages Bayes’ Rule to estimate lithology probability given electrical resistivity. The simplest approach (method 1) yields an erroneous conditional probability function where sand dominates the conditional probability at nearly all resistivities, due in part to the strong presence of sand in the wells nearest the ERT lines. The approaches using MLE and lithology simulations (methods 2 and 3) produce similar, more realistic lithofacies probability functions. The range of resistivities where clay and sand overlap differs between methods 2 and 3: ranging between 100 and 200 ohm-m for method 2, and between 30 and 50 ohm-m for the method 3. These differences affect the posterior lithology distributions in multiple point geostatistical (MPS) simulations, and in turn, predictions of flow from models which integrate these results. To test the models, we can compare measured breakthrough times of recharged water at the site to groundwater flow simulation results using the lithofacies models created by each method. The methods described here can inform the integration of non-collocated geophysical data into a variety of applications. [less ▲]

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See detailThe effect of initial water distribution and spatial resolution on the interpretation of ERT monitoring of water infiltration
Dumont, Gaël ULiege; Pilawski, Tamara ULiege; Robert, Tanguy et al

Poster (2017, July 25)

A better understanding of the water balance of a landfill is crucial for its management, as the waste water content is the main factor influencing the biodegradation process of organic waste. In order to ... [more ▼]

A better understanding of the water balance of a landfill is crucial for its management, as the waste water content is the main factor influencing the biodegradation process of organic waste. In order to investigate the ability of long electrical resistivity tomography (ERT) profiles to detect zones of high infiltration in a landfill cover layer, low resolution time lapse data were acquired during a rainfall event. Working at low resolution allows to cover large field areas but with the drawback of limiting quantitative interpretation. In this contribution, we use synthetic modeling to quantify the effect of the following issues commonly encountered when dealing with field scale ERT data: (i) the effect of low resolution on electrical resistivity changes interpretation, (ii) the effect of the original heterogeneous resistivity distribution on the observed relative resistivity changes, (iii) the need for temperature and pore fluid conductivity data in order to compute water content and absolute changes of water content, and (iv) the interpretation error commonly made while neglecting the dilution effect during fresh water infiltration. Firstly, due to the lack of spatial resolution, the regularized inversion process yields a smoothed distribution of resistivity changes that fail to detect small infiltration zones and yields an overestimation of the infiltration depth and an underestimation of the infiltrated volume in large infiltration areas. Secondly, the analysis of relative changes, as commonly used in literature, is not adequate when the background water content is highly heterogeneous. In such a case, relative changes reflect both the initial water content distribution and the observed changes. Thirdly, the computation of absolute water content changes better reflects the infiltration pattern, but requires spatially distributed temperature and pore fluid conductivity input data. Lastly, the dilution effect, if not considered, leads to an underestimation of the infiltrated volume. Taking into account these elements, we extracted the maximum amount of information from our field data without over-interpreting the results. This allowed the detection of larger infiltration areas possibly responsible for a large part of the annual water infiltration and landfill gas loss. [less ▲]

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See detailA panmictic Amazonian world? : Bryophytes testify
Ledent, Alice ULiege

Poster (2017, July 25)

Understanding connectivity over different spatial and temporal scales is fundamental for biodiversity conservation and management. The Amazonian rainforest, one of the most diverse biodiversity hotspots ... [more ▼]

Understanding connectivity over different spatial and temporal scales is fundamental for biodiversity conservation and management. The Amazonian rainforest, one of the most diverse biodiversity hotspots, has experienced dramatic range contractions and expansions due to Pleistocene climate oscillations, and its human-induced fragmentation has accelerated at an unparalleled pace in the course of the Anthropocene. In this context, epiphytes, with their relatively short life-cycles, offer an ideal model to investigate the impact of past and present fragmentation on patterns of genetic structure and diversity. Due to the necessity to switch from one host tree to another, or from one leaf to another, epiphytic bryophytes typically exhibit high dispersal syndromes. In line with such high dispersal capacities, recent metacommunity analyses have arisen the intriguing question that Amazonian epiphytic bryophyte communities are homogeneous across very large spatial scales, ultimately raising the notion that they might behave as a basin-wide panmictic population. Here, we implement fine-scale population genetic analyses to address the following questions:(i) Do Amazonian epiphytes exhibit population structure at regional (< 500 km) scale; (ii) If the hypothesis of a panmictic population is rejected, (iia) at which spatial scale does genetic structuring occur, and (iib) do neutral (isolation-by-distance) or ecological (isolation-by-ecology) processes shape patterns of genetic variation? We sampled exemplars of 15 epiphytic bryophyte species from two ecologically contrasted forest types (lowland rainforest and white-sand forest) in a 50,000 km2 area in the middle Rio Negro. Genome-wide genetic data were produced using Genotyping By Sequencing. To circumvent severe taxonomic issues in challenging groups, which, like the Calymperaceae, are dominant in the epiphytic flora, we first implemented species delimitation analyses to sort-out specimens taxonomically. We then described the fine-scale genetic structure of each species and performed isolation-by-distance analyses to detect significant spatial genetic structuring. We finally determined whether isolation-by-distance or ecological filtering contribute to the observed patterns of genetic variation. The study will provide key information on the populations dynamics of highly mobile species integral to the iconic Amazonian forest, which may further be employed to refine future conservation policies in the face of accelerating climate change and anthropogenic-mediated deforestation. [less ▲]

Detailed reference viewed: 21 (3 ULiège)
See detailDual-specificity-phosphatase 3 (DUSP3) deletion promotes liver inflammation and high fat diet-induced hepatocellular carcinoma
Jacques, Sophie ULiege; Arjomand, Arash ULiege; Vandereyken, Maud ULiege et al

Poster (2017, July 24)

Overweight and obesity are considered as risk factors for hepatocellular carcinoma (HCC) development. The mechanisms by which obesity promotes liver inflammation are however poorly understood. We recently ... [more ▼]

Overweight and obesity are considered as risk factors for hepatocellular carcinoma (HCC) development. The mechanisms by which obesity promotes liver inflammation are however poorly understood. We recently generated a full DUSP3 knockout (KO) mouse. The obtained mice were born normal with no spontaneous phenotype. However, while aging, DUSP3-KO mice became obese and developed hepatosteatosis. The phenotype was exacerbated under high fat diet (HFD). Furthermore, when treated with diethylnitrosamine (DEN) procarcinogen, DUSP3-KO mice developed HCC faster than WT littermates. The combination of DEN with HFD accelerated the onset of HCC development in these mice compared to WT mice. This was associated with increased systemic levels of several metabolites and with hyperphosphorylation of the insulin-like growth factor receptor I. [less ▲]

Detailed reference viewed: 24 (1 ULiège)
See detailFunctional and structural characterisation of the Arabidopsis thaliana HMA4 protein
Lekeux, Gilles ULiege; Laurent, Clémentine; Xiao, Zhiguang et al

Poster (2017, July 24)

Detailed reference viewed: 30 (1 ULiège)
Peer Reviewed
See detailItem purification versus adjustments for multiple comparisons in DIF detection
Drabinova, Adela; Martinkova, Patricia; Magis, David ULiege

Poster (2017, July 18)

Detailed reference viewed: 28 (0 ULiège)
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See detailA Novel Accelerometer-Based Method for Stride Length Estimation
Boutaayamou, Mohamed ULiege; Schwartz, Cédric ULiege; Denoël, Vincent ULiege et al

Poster (2017, July 14)

We demonstrate the feasibility of accurately and precisely estimating the left/right average stride length from measured heel/toe accelerations in the gait of healthy, old adults. Our approach relies on ... [more ▼]

We demonstrate the feasibility of accurately and precisely estimating the left/right average stride length from measured heel/toe accelerations in the gait of healthy, old adults. Our approach relies on (1) a novel method that uses only accelerometer data without the need of additional data from, e.g., gyroscopes and/or magnetometers, and on (2) the validation of the results using reference 3D optoelectronic system data. [less ▲]

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See detailNon-pathological aging of the executive functions: influence of the Val108/158Met nucleotide polymorphism of the COMT gene
Gilsoul, Jessica ULiege; Manard, Marine ULiege; Collette, Fabienne ULiege

Poster (2017, July 13)

Objectives. The aim of the study is to investigate the influence of the COMT Val108/158Met nucleotide polymorphism, associated with dopaminergic regulation, on executive functions in normal aging. Indeed ... [more ▼]

Objectives. The aim of the study is to investigate the influence of the COMT Val108/158Met nucleotide polymorphism, associated with dopaminergic regulation, on executive functions in normal aging. Indeed, according to the Dual-State Theory, Val/Val polymorphism of the COMT gene would favor better flexibility abilities, whereas Met/Met carriers would favor better stability abilities. These genotypic effects should be magnified in older people, as a decrease in cognitive resources increases the influence of individual genetic differences on cognitive performance. Method. We assessed the executive processes of inhibition, shifting, and updating – thanks to many different tasks in groups of young (n = 55) and aged participants (n = 45) carrying one of the three polymorphism of the COMT gene (Val/Val, Val/Met, Met/Met). T-tests between our two groups (Young versus Aged) but also a 6 (Group: Young-Met/Met, Young-Val/Met, Young- Val/Val, Old-Met/Met, Old-Val/Met, and Old-Val/Val) X 3 (Executive functioning: Inhibition, Shifting, and Updating) repeated measure ANOVA with Executive functioning as within-subject factor (p < .05) were carried out. Results. Young and old participants significantly differed on the three executive functions. The comparison of genotypic groups showed no significant differences in young or older participants respectively. Interestingly, the Old-Val/Val group showed a lower performance than the young one on inhibition tasks. Conclusion. As expected, we found an age effect on all the executive functions. The significant difference between the Young-Val/Val and the Old-Val/Val groups on inhibition suggests an age-related decrease in abilities requiring a stability component in individuals possessing a less favoring genotype for this component. [less ▲]

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See detailExtraction-column design for highly viscous systems
Quaresima, Maria Chiara ULiege; Pfennig, Andreas ULiege

Poster (2017, July 11)

The change from fossil-based feedstock to bio-based raw materials will lead to changes in the molecular structure of reagents used in the chemical industry. Bio-based raw materials are richer in oxygen ... [more ▼]

The change from fossil-based feedstock to bio-based raw materials will lead to changes in the molecular structure of reagents used in the chemical industry. Bio-based raw materials are richer in oxygen, leading to intermediates and products rich in oxygen as well. This will lead to lower vapor pressure and higher viscosity of the systems. Thus liquid-based separations like solvent extraction will increase in importance. Also separation-process design needs to be adapted to properly account for higher viscosities. With drop-based simulation, extraction-column performance can be predicted to better than 10% accuracy, being time and resources saving compared to pilot-plant experiments. In previous work, appropriate models describing all drop phenomena like sedimentation and mass transfer were combined in a simulation tool, called ReDrop (REpresentative DROPs). The main idea of ReDrop is to follow the behavior of a sufficiently large number of individual drops along their path through the column and solve the population balance accounting for the interconnected phenomena mentioned. The models implemented in ReDrop have been largely validated for low-viscous systems. In order to extend the capabilities of ReDrop and describe the entire viscosity range, models have to be validated for a variety of different material system with single-drop lab-scale experiments. Sedimentation velocity and mass transfer of a single drops have been studied with a single-drop cell, where a conical glass tube is used to levitate the drop by a counter flow of continuous phase. The single-drop results are the basis for validation and extending the applicability of existing models to higher viscosity, which are then introduced into ReDrop. [less ▲]

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See detailDetermining fine population structure using iterative pruning
Chaichoompu, Kridsadakorn ULiege; Yazew, Fentaw Abegaz; Tongsima, Sissades et al

Poster (2017, July 10)

SNP-based information is used in several existing clustering methods to detect shared genetic ancestry or to identify population substructure (Price et al. 2006, Raj et al. 2016). Here, we present an ... [more ▼]

SNP-based information is used in several existing clustering methods to detect shared genetic ancestry or to identify population substructure (Price et al. 2006, Raj et al. 2016). Here, we present an unsupervised clustering algorithm called the iterative pruning method to capture population structure (IPCAPS). Our method supports ordinal data which can be applied directly to SNP data to identify fine-level population structure and it is built on the iterative pruning Principal Component Analysis (ipPCA) algorithm (Intarapanich et al. 2009). The IPCAPS involves an iterative process using multiple splits based on multivariate Gaussian mixture modeling of principal components and Clustering EM estimation as in Lebret et al. (2015). In each iteration, rough clusters and outliers are also identified using our own method called RubikClust. The fixation index (FST) is known to measure a distance between populations and FST = 0.001 may be said to be genetically distinct among the European populations (Tian et al. 2008, Huckins et al. 2014). To observe fine-level population structure using FST, we examined simulated scenarios of one population, 500-8,000 individuals, 5,000-10,000 independent SNPs in HWE (Balding and Nichols 1995), with 100 replicates for each scenario. The simulated SNPs were encoded as additive coding and there was no missing genotype generated. We introduced negative control by subjecting individuals to be separated into two groups using kmeans. We observed that FST values of divided groups were lower than 0.0008, which can be defined as the minimum FST to detect fine-level population structure. To evaluate the performance of our method, we tested different simulated data sets of 2-3 populations, 250 individuals per population, 10,000 independent SNPs in HWE, and FST=[0.0008,0.005], with 100 replicates for each data set. For real-life data sets, we applied the IPCAPS to Thai (Wangkumhang et al. 2013) and HapMap populations. Our method showed that a population classification accuracy was superior to the ipPCA in simulated scenarios of extremely subtle structure (FST=[0.0009,0.005]). In case of the Thai population, results to detect fine-level structure were obtained as well as in case of the HapMap populations. We are convinced that the IPCAPS has a potential to detect fine-level structure and it will be important in molecular reclassification studies of patients once underlying population structure has been removed. [less ▲]

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See detailSTUDY OF ACTIVATED SLUDGE DEWATERING : USING BIOBASED COAGULANTS
Fraikin, Laurent ULiege; Clermont, Quentin; Salmon, Thierry ULiege et al

Poster (2017, July 06)

This paper focuses on the dewatering efficiency of activated sludge from waste water treatment plant using biobased coagulants in addition with classical flocculant and compares the results with those ... [more ▼]

This paper focuses on the dewatering efficiency of activated sludge from waste water treatment plant using biobased coagulants in addition with classical flocculant and compares the results with those obtained with a sole classical coagulant. For this work, a standard compression-expression cell is used and an experimental design is develop to highlight the effect of the biobased coagulants. [less ▲]

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See detailContinuous-flow preparation of γ-butyrolactone scaffolds from renewable fumaric and itaconic acids under photosensitized conditions
Gerardy, Romaric ULiege; Winter, Marc; Horn, Clemens R. et al

Poster (2017, July 06)

Detailed reference viewed: 6 (2 ULiège)
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Peer Reviewed
See detailComparative Proteomics Analysis Provides New Candidates for Zinc Homeostasis Regulation in Arabidopsis
Amini, Sahand ULiege; Arsova, Borjana ULiege; Scheepers, Maxime ULiege et al

Poster (2017, July 03)

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants ... [more ▼]

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants at high concentrations in soils. This calls for a better unravelling of Zn homeostasis regulation mechanisms, including sensing and signaling in plants. In order to fulfill this aim, we are testing for novel proteins involved in Zn homeostasis in the model plant Arabidopsis thaliana. First, quantitative proteomics was performed on root and shoot samples obtained upon Zn starvation and re-supply in different spatio-temporal conditions. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was also performed for those treatments to measure the Zn concentration in tissues. It showed very rapid Zn uptake in root upon re-supply. Moreover, quantitative expression studies of known players of Zn homeostasis confirmed our large-scale proteomic results, although for a few genes lack of correlation between transcript and protein regulation was observed. Using clustering, statistical and gene ontology analyses, we selected candidate genes for further studies. Among more than 5000 detected proteins in roots by shotgun proteomics, 75 genes were selected for targeted analyses. In general, our results show that comparative proteomics study can be useful to reveal new players in the Zn regulatory network in plants, which can lead to new Zn biofortification and phytoremediation strategies. [less ▲]

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See detailSimultaneous Determination of Free Amino Acid Content in Different Teas using Supercritical Fluid Chromatography Coupled with Single Quadrupole Mass Spectrometry
Huang, Yang ULiege; Fillet, Marianne ULiege; Crommen, Jacques ULiege et al

Poster (2017, July 02)

Tea (Camellia sinensis L.) is a complex mixture containing a wide range of biological activities and has been used as widely consumed beverages and natural medicine for over thousand years [1-2]. In this ... [more ▼]

Tea (Camellia sinensis L.) is a complex mixture containing a wide range of biological activities and has been used as widely consumed beverages and natural medicine for over thousand years [1-2]. In this study, a novel method for high-performance liquid chromatography with mass spectrometry (SFC-MS) has been developed to simultaneously determine the contents of 11 free amino acids in different types of teas (pu-erh tea, green tea, black tea and oolong tea). The separation conditions for the selected amino acids were carefully optimized such as the column type, temperature and backpressure, the type of additive. The best compromise for tested analytes in terms of chromatographic performance was obtained when water (5%) and trifluoroacetate acid (0.4%) were added to the supercritical carbon dioxide/methanol mobile phase. Finally, the developed SFC-MS method was successfully applied to the analysis of the 11 amino acids present in the teas and fully validated as well. The results indicated a good linearity (r ≥0.995), precision (RSD≤ 2.99%), stability (RSD≤ 2.88%) and accuracy (91.95%~107.99%). The limits of detection ranged from 1.42 to 14.69 ng/mL, respectively, while the limits of quantification were between 4.53 and 47.0 ng/mL. The content of the amino acids in six different tea samples were also determined and presented some difference basing on the fermentation processes. The proposed SFC-MS method showed a great potential in further investigations to differentiate tea varieties [less ▲]

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See detailCANINE GANGRENOUS MASTITIS : A CASE REPORT
Egyptien, Sophie ULiege; Lefebvre, Michaël ULiege; Guieu, Liz-Valéry ULiege et al

Poster (2017, July)

This report describes the treatments and discusses the cost effectiveness of a Negative Pressure Wound Therapy (NPWT) device on a gangrenous mastitis case. Mastitis is an inflammation of the mammary gland ... [more ▼]

This report describes the treatments and discusses the cost effectiveness of a Negative Pressure Wound Therapy (NPWT) device on a gangrenous mastitis case. Mastitis is an inflammation of the mammary gland found mainly in lactating females. Coliforms (Escherichia coli), Staphylococcus spp (Staphylococcus aureus) and, to a lesser extent, Streptococcus spp are the most commonly isolated organisms in bitches. The bitch can be presented because of local signs of inflammation, puppies failing to thrive or even severe septic shock. While more common in ruminants, gangrenous mastitis is rare in the bitch. It is mostly due to Staphylococcus aureus, a gram positive and catalase positive bacteria producing alpha hemolysin. This toxin binds to ADAM10, a transmembrane protein, forming pores in the cell membrane, causing an acute inflammation via intracellular calcium flow, leading to severe oedema, necrosis and gangrene. Loss of skin integrity also comes from the cleavage of E-cadherin by ADAM10 after its toxin-mediated activation. A 8.7kg, 3-year-old neutered female Sheltie farm dog was referred to the Veterinary Clinic of the University of Liège for suspicion of septic peritonitis after an ovariohysterectomy on 4 dead puppies 36 hours earlier. They died during long lasting dystocia. Amoxicillin-acid clavulanic and enrofloxacin antibiotherapy had been initiated during surgery. The dog was in decompensated septic shock. The surgical wound was not reactive and no sign of peritonitis was seen by abdominal ultrasonography. The right inguinal and the two most caudal left mammary glands were swollen, crackling with a patchy blue discoloration. An acute fulminant mastitis with gangrenous involvement and sepsis was diagnosed. Fluids and continuous rate infusion of norepinephrine and dobutamine were administered to control severe hypotension. Metronidazole was added since Clostridium spp could not be excluded. Debridement was started as soon as the arterial pressure was stabilised and the wound was disinfected with hydrogen peroxyde for the 3 first days. While hydrogen peroxyde is indicated for catalase negative infections, the already started antibiotherapy prevented bacterial identification and sensitivity determination. Alginate-honey patches replaced wet to dry bandages after 4 days. After 7 days of progressive debridement, the wound was 15cm long, 13cm wide and 1.2cm deep. A NPWT device was placed under anaesthesia after extensive debridement. It was kept in place for one week with one renewal after 2 days under slight sedation. The wound was then surgically closed. Cutaneous sutures were removed 10 days later as cicatrization was satisfactory. NPWT is indicated in open wound management, for infection control and for stimulating granulation tissue production prior to reconstruction. It increases contraction in deep, three dimensional wounds. Closure rate is significantly shorter with NPWT than with standard wet to dry bandages. Cost of NPWT is comparable to standard bandages but more comfortable for the patient. Standards bandages require daily changes, sometimes for several weeks and under repeated anaesthesias, which may be debilitating for the patient and expensive for the owner. Conversely, NPWT is changed only once and allows earlier surgical closure of the wound. The main complication is vacuum loss, which is surgeon’s experience dependant. [less ▲]

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See detailStable isotopes reveal effects of environmental changes on ecological niches of Iphimediidae amphipods
Michel, Loïc ULiege; d'Udekem d'Acoz, Cédric; Frederich, Bruno ULiege et al

Poster (2017, July)

When faced with environmental changes, organisms are expected to have some intrinsic ability to adapt through ecological plasticity. However, this process is still poorly understood in many Antarctic ... [more ▼]

When faced with environmental changes, organisms are expected to have some intrinsic ability to adapt through ecological plasticity. However, this process is still poorly understood in many Antarctic invertebrates. Here, we focused on Iphimediidae amphipods, as this widely distributed family shows important ecological diversity. In total, 248 amphipods (19 species) from two widely different zones (the West Antarctic Peninsula, or WAP, and Adélie Land, AL) were studied to elucidate how environment can influence ecological niche parameters. Ecological niches were explored using stable isotope ratios of carbon and nitrogen and the SIBER approach (Jackson et al., 2011). The isotopic niche of the whole amphipod assemblage was wider in WAP than in AL. This was true for both total (proxy of the whole range of resources exploited by animals) and the core (proxy of the most commonly used resources) isotopic niches. The ratio between total and core isotopic niches was smaller in WAP than in AL (4.13 vs. 5.74), suggesting that in WAP, animals commonly use a greater relative percentage of the resources to which they have access. Niche modelling at the specific level revealed that this trend was not found in all taxa. For example, niches of Gnathiphimedia sexdentata and Iphimediella microdentata were bigger in WAP than in AL, following the general pattern. On the other hand, niches of Echiniphimedia echinata and E. hodgsoni had the same width in both areas. Moreover, relative niche overlap between these two species was much higher in WAP (42%) than in AL (20%). Our results indicate that the widely different environmental conditions encountered by the animals in these two zones clearly influence their ecology. Overall, Iphimediidae amphipods tend to exploit more resources in WAP, i.e. in the zone where impacts of global change (temperature increase, sea ice cover decrease) are the strongest. Niche overlap between some closely related (i.e. congeneric) species was also more important in WAP. Ultimately, environmental changes in this region might reinforce these trends, which might lead to competition and perturb amphipod community structure. This research was funded by the Belgian Federal Science Policy Office (BELSPO) in the framework of the vERSO and RECTO projects. [less ▲]

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Peer Reviewed
See detailClinical outcome after tracheal resection and anastomosis for correction of a tracheal stenosis in a sport pony
Salciccia, Alexandra ULiege; Roose, Charlotte; Joostens, Zoé ULiege et al

Poster (2017, July)

Introduction: Tracheal stenosis, which refers to a narrowing of the tracheal lumen, is infrequent in horses. The literature is scarce about outcome after treatment of this pathology. Objectives: To report ... [more ▼]

Introduction: Tracheal stenosis, which refers to a narrowing of the tracheal lumen, is infrequent in horses. The literature is scarce about outcome after treatment of this pathology. Objectives: To report our experience using tracheal resection and anastomosis to treat a severe trauma-induced tracheal stenosis (< 1 cm diameter of lumen) involving 2 tracheal rings causing a loud respiratory noise at rest in a 14 y.o. event pony. Methods: The pony was trained to wear a martingale preoperatively. Before the surgery, a distal tracheotomy was performed for placement of the tracheal tube for maintenance of gaseous anaesthesia. Through a 35 cm ventral midline cervical incision, the trachea was exposed and separated from adjacent tissues. Stay sutures were placed in tracheal cartilage adjacent to the segment to be removed. The 2 tracheal rings involved in the stricture were subsequently removed. On both remaining segments of the trachea, the mucosa was turned back over the open end and sutured to the adventitia. The head was then flexed and tracheal ends were apposed using 5 stainless steel wires placed equidistantly around the trachea without mucosal penetration. The anastomosis was completed by a simple continuous suture of resorbable material on the adventitia. A closed suction drain was applied in the soft tissue before routine closure of the subcutaneous and cutaneous layers. The martingale was applied before the recovery from anaesthesia, which was uneventful. The tracheotomy tube and the suction drain were left in place for 48h. The martingale was removed after 3 weeks. The pony was discharged 1 month after surgery. Control endoscopies were performed during hospitalisation and at 7, 10, 17, 21 and 30 weeks and 1 year after surgery. Results: A moderate cicatrix recurred. The excessive tissue was injected with triamcinolone and then with 4% formaldehyde during the first endoscopies and remained stable thereafter. Some stainless steel cerclages broke and displaced slightly. This caused the formation of a self-resolving seroma caudally to the trachea. A moderate respiratory noise was still audible at low-intensity work, which was resumed 4 months postoperatively. However, work was stopped before achieving the preoperative level because the pony developed a lameness. Conclusions: Although a moderate tracheal cicatrix recurred after surgery, tracheal resection and anastomosis clearly improved the quality of life of this pony. [less ▲]

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See detailThe question of the methane increase through the retrieval of its isotopologues from FTIR ground-based observations
Bader, Whitney ULiege; Strong, Kimberley; Walker, Kaley

Poster (2017, July)

Methane (CH4) is the second most important greenhouse gas emitted by human activities in the Earth’s atmosphere. Although it is roughly 200 times less abundant than carbon dioxide, it is a 28 times more ... [more ▼]

Methane (CH4) is the second most important greenhouse gas emitted by human activities in the Earth’s atmosphere. Although it is roughly 200 times less abundant than carbon dioxide, it is a 28 times more potent greenhouse gas. Approximately one fifth of the changes in the Earth’s balance energy caused by human-linked greenhouse gases since the beginning of industrialization (~1750) is due to methane. Methane is emitted by both natural sources and human activities. Indeed, methane can be emitted to the atmosphere through coal mining, oil and gas exploitation, rice cultures, domestic ruminant animals, biomass burning, waste management, wetlands, termites, methane hydrates and ocean. Since the beginning of the industrialization, atmospheric methane concentrations have increased by 260% to reach 1824 pbb in 2013. From the 1980s until the beginning of the 1990s, atmospheric methane was significantly on the rise, then stabilized during 1999-2006 to rise again afterwards. To this day, the source or sink responsible of this latter increase remains unexplained. Through each emission process, heavy molecules of methane (with one additional neutron either on a carbon or on one hydrogen atom) are emitted along methane (12CH4). The main heavy molecules of methane, called isotopologues (13CH4 and CH3D), are respectively ~110 and ~60 000 times less abundant than methane. Despite their small abundances, they give crucial information on the concentration of methane in the atmosphere and its evolution. Indeed, both isotopologues are emitted with specific emission ratio depending on the emission sources. Determining isotopic ratio of atmospheric methane is therefore a unique tracer of its budget. While the non-monotonous trend of methane is subject of an extensive number of studies, to our knowledge, no study of the isotopic ratio of methane derived from ground-based solar observations has been published to date. Measurements of heavy methane from Fourier Transform InfraRed spectra recorded with state of the art spectrometers installed at Eureka [Arctic, Canada] and Toronto [Ontario, Canada] will help fill this gap. Indeed, the produced time series will ease data interpretation and contribute to a global view of the question of isotopologues. [less ▲]

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See detailBeta Cell Regeneration in Zebrafish : Investigating the Ductal Contribution
Bergemann, David ULiege; Massoz, Laura; Bourdouxhe, Jordane ULiege et al

Poster (2017, July)

Diabetes is characterized by the loss of insulin producing beta cells. Although different therapeutic strategies do exist, they lack precise and dynamic control of glycemia as carried out by endogenous ... [more ▼]

Diabetes is characterized by the loss of insulin producing beta cells. Although different therapeutic strategies do exist, they lack precise and dynamic control of glycemia as carried out by endogenous beta cells. One promising alternative is to replenish the pancreas with bona fide functional beta cells by triggering regeneration mechanisms. Previous studies have shown beta cell neogenesis originating from different pancreatic cell types (alpha, delta, acinar and ductal cells), depending on the used model. The ductal origin is supported by the fact that the embryonic duct epithelium gives rise to the endocrine lineage, and that in healthy and diabetic human adults, insulin positive cells could be found next to or in pancreatic ducts. Despite these observations, mammals show very limited regenerative capabilities, making it difficult to investigate those mechanisms. In contrast, zebrafish are extensively used for regeneration studies. The ability of adult zebrafish to regenerate its beta cells and restore normoglycemia after massive beta cell ablation has already been shown. We, and other groups, have previously shown that adult pancreatic duct cells act as progenitors, giving rise to beta cells in physiological and induced diabetic condition in vivo. To get insight into this process, we conducted comparative RNA-seq experiments on pancreatic duct cells from adult zebrafish. By this means, we identified regulated gene expression that can be linked to specific processes/pathways such as cell cycling and Notch. In order to confirm the involvement of identified candidate genes/pathways, we are setting up a screening method using chemical activators and inhibitors in old larvae, by looking at their ability to modify proliferation/differentiation of duct cells after beta cell ablation. In addition, we are also investigating the effect of mutations, generated by the CRISPR/Cas9 system within candidate genes, on the regeneration capabilities. Together, these strategies should provide new clues about regenerative processes triggered in duct cells that might be applied to overcome the poor regenerative capabilities of mammals. [less ▲]

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See detailImpact of endophytic entomopathogenic fungus on plant attractivity in aphid-virus-plant interactions
Fingu Mabola, Junior Corneille ULiege; Bosquée, Emilie ULiege; Boullis, Antoine ULiege et al

Poster (2017, July)

Endophytic entomopathogenic fungi (EEPF) are biological control agents able to live in their host plants in a symbiotic interaction and secrete secondary metabolites in the host plant tissues interacting ... [more ▼]

Endophytic entomopathogenic fungi (EEPF) are biological control agents able to live in their host plants in a symbiotic interaction and secrete secondary metabolites in the host plant tissues interacting with pests and phytopathogenic agents like plants virus. This is the case of Barley yellow dwarf virus (BYDV) transmitted by the Bird cherry oat aphid (Rhopalosiphum padi Linnaeus) and the Grain aphid (Sitobion avenae Fabricius). This virus is one of the most economically important phytoviruses affecting major world cereal crops such as wheat, rice and corn, causing significant yield losses. In this study, we investigated the influence of EEPF presence in the plant tissues on the vector’s orientation preference by considering the insect virus-infection status. We performed a choice test in Petri dishes and collection of volatile compounds emitted by plants. The impact of these interactions between plant-fungi-virus is finally discussed. [less ▲]

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See detailLanguage abilities in preterm-born children : Specific difficulties in phonological skills
Desmottes, Lise ULiege; Maillart, Christelle ULiege; VIELLEVOYE, Renaud ULiege et al

Poster (2017, July)

Children who were born prematurely (before 37 weeks’ gestation) are at greater risk for a range of impairments in cognitive, motor, social-emotional, and academic functioning (e.g., Bos et al., 2013 ... [more ▼]

Children who were born prematurely (before 37 weeks’ gestation) are at greater risk for a range of impairments in cognitive, motor, social-emotional, and academic functioning (e.g., Bos et al., 2013). Other developmental disorders, such as language delays and deficits, are also more common in preterm children (Van Noort et al., 2012). Because language function is essential in all kinds of social and academic life (Young et al., 2002), it is crucial to better identify and characterize language impairment in preterm-born children. Despite the large amount of research conducted on language abilities in preterm children, little is known about their phonological development (Barre et al., 2011). In the present study, we investigated differences in several phonological tasks between French-speaking premature children and in term-born control children. 30 children born at <35 weeks’ gestation (4 to 5 years old) and with low-birth weight (1215 g on mean) were recruited. These preterm children were matched to 30 children born at term on gender, chronological age and socio-economic level. All participants were administered five language tasks (phonemic discrimination, phonological judgment, pictures naming, pseudo-words repetition and phonological awareness) in order to precisely assess their phonological skills. Results indicated that preterm children presented poorer performance than control term-born children when they had to discriminate between different phonemes (phonemic discrimination abilities), to detect phonemic modifications (phonological judgment) and to recognize and identify syllabic segments (phonological awareness). Differences between both groups have also been observed in the quality of phonological representations (pictures naming task). However, both groups of children performed equally well in the pseudo-words repetition task. These findings revealed that preterm children score significantly lower compared with term-born children on several phonological tests, a language subdomain which is particularly important in the initial stages of language development and for reading. [less ▲]

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See detailLight Induced Super-Hydrophilicity in Niobium and Tantalum Oxides Thin Films
Henry, Théo ULiege; Bonsague, Fabio; Dubreuil, Olivier ULiege et al

Poster (2017, July)

Since its discovery at the end of the nineties by Wang et al.,[1] the interest for light-induced superhydrophilicity is ever growing. The application fields for materials displaying such a property are ... [more ▼]

Since its discovery at the end of the nineties by Wang et al.,[1] the interest for light-induced superhydrophilicity is ever growing. The application fields for materials displaying such a property are wide and numerous:[2] self-cleaning surfaces, anti-fogging, anti-bacterial, water and air purification, microfluidics…Nowadays, a research emphasis is on relatively low energy bandgap semiconductors, targeting a higher efficiency under solar irradiation or more precisely in the near UV and low-end of the visible spectral range. Here, however, we report UV-induced superhydrophilicity in metal oxides thin films showing a relatively high transparency in the UV. Reactive ion beam sputtering (IBS) was used to deposit niobium, tantalum and titanium oxides thin films with thickness ranging from 10 nm to 120 nm on fused silica substrates at room temperature. Contact angle measurements were performed for progressively increasing UV doses and also as function of elapsed time after the termination of the exposure in order to investigate the UV-induced hydrophilicity and recovery behavior of tantalum and niobium oxide thin films in comparison to that of titanium oxide deposited in analogous conditions. [less ▲]

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See detailAn overview of the quality of interactions in Belgian pre-kindergarten classrooms
Leroy, Sandrine ULiege; Desmottes, Lise ULiege; Bergeron-Morin, Lisandre ULiege et al

Poster (2017, July)

Quality of teacher-children interactions during early childhood plays a protective effect on language development, more particularly with the more vulnerable children (Dickinson, 2011). However, the ... [more ▼]

Quality of teacher-children interactions during early childhood plays a protective effect on language development, more particularly with the more vulnerable children (Dickinson, 2011). However, the quality of these interactions, especially those supporting language development, is not always optimal (Piasta et al., 2012). It is of great concern in Belgium, where there is a need to prevent, during early childhood, an educational gap that too often depends on the socio-economic status of a child’s family (Vandenbroeck, 2015). Supporting the quality of teacher-children interactions in kindergarten may be a way to preventively support language development and educational achievement. But to date, little is known about what children experience in Belgium kindergarten, regarding language development support. The objective of this study is to document the quality of teacher-children interactions, in the second year of nursery school (4-5 years old) in the French-speaking part of Belgium. Observations take place in 23 classes. The CLASS Pre-K (Classroom Assessment Scoring System®, Pianta et al., 2008) is used for measuring the quality of teacher-children interactions. Trained observers have assessed quality of interactions along three domains: Emotional Support, Classroom Organization and Instructional support including language modeling. Results have revealed a lower quality in instructional support while showing a medium-high quality in the emotional support and classroom organization. However, scores in the instructional support increase when teachers propose activities deliberately dedicated to language. A more detailed analysis of the observations in 23 classes, in relation with number of children in the classroom and type of proposed activities will be discussed. These results will be interpreted within the particular context of kindergarten in Belgium. The present study aims at enabling a more global reflection on the modalities that can better support language development in an educational context, more particularly with more vulnerable children. [less ▲]

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See detailLanguage and analogical reasoning in children with Specific Language Impairment: The effect of articulatory suppression
Krzemien, Magali ULiege; Thibaut, Jean-Pierre; Stoffels, Valentine ULiege et al

Poster (2017, July)

Analogical reasoning shares a mutual influence with language development: analogical reasoning is improved by labelling analogical items with words describing the relations that they contain. Conversely ... [more ▼]

Analogical reasoning shares a mutual influence with language development: analogical reasoning is improved by labelling analogical items with words describing the relations that they contain. Conversely, structural alignment, a core mechanism of analogical reasoning, allows the acquisition of novel words and the development of grammar (Gentner, 2010). Given those findings, some authors have taken interest in the analogical reasoning ability of children with language disorders, and specifically of children with Specific Language Impairment (SLI). Those children have worse performance than their age-matched peers without language disorders in linguistic or non-linguistic analogical tasks (Leroy, Maillart, & Parisse, 2014). Our aim here is to see if this weakness is due to their language disorders and if they use the same verbal strategies as their peers to solve an analogical task. To experience these assumptions, we use a perceptual analogical task following an A:B::C:D paradigm: participants have to find the relation between two geometric forms A and B and to apply it to the C term in order to find the D term among distractors. The distractors either share perceptual features with the C term, what creates a competition that shall be inhibited, or not. Moreover, children are faced with three interfering task conditions: one without any interfering task, one with an articulatory suppression secondary task and one with a tapping secondary task, which is used in order to measure the general dual task demands. Comparing the results of these conditions will allow us to evaluate the impact of language and verbal strategies on analogical reasoning in control and SLI children. [less ▲]

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See detailAntarctic sea ice trophic status
Van der Linden, Fanny ULiege; Moreau, Sébastien; Champenois, Willy ULiege et al

Poster (2017, July)

The sea ice ecosystem is characterized by steep gradients in temperature, salinity, light and nutrient availability. Despite these challenging environmental conditions, sea ice provides a dynamic habitat ... [more ▼]

The sea ice ecosystem is characterized by steep gradients in temperature, salinity, light and nutrient availability. Despite these challenging environmental conditions, sea ice provides a dynamic habitat for diverse communities of microorganisms. These communities include a wide variety of organisms from different taxonomic groups such as algae, bacteria, heterotrophic protists, fungi as well as viruses [Horner et al., 1992; Deming, 2010; Thomas and Dieckmann, 2010; Poulin et al., 2011]. In the frame of the YROSIAE project (Year-Round survey of Ocean-Sea-Ice-Atmosphere Exchanges), carried out at Cape Evans in McMurdo Sound (Antarctica) from Nov. 2011 to Dec. 2012, ice cores, seawater, and brine material were collected at regular time intervals. Physical properties (salinity, temperature, texture) and biogeochemical parameters (pCO2, dissolved inorganic carbon, total alkalinity, chlorophyll-a, macro-nutrients) were analysed. We will here particularly consider changes inused dissolved inorganic carbon (DIC) and chlorophyll-a (chl-a) , used as a proxiesy of net community production and autotrophic biomass, respectively. A high spatial and temporal variability in ice algal biomass and DIC evolution were observed. From spring, very high chl-a concentrations (>2400μg.L^(-1)) were observed at the bottom of the ice, a common feature of land fast ice in the McMurdo Sound. This suggests high primary production. However Strikingly, , at the same time, nutrients at the bottom of the ice increased significantly suggesting high heterotrophyremineralisation. In the middle of the ice column, evolution of DIC is was marked by a succession of autotrophic and heterotrophic phases. The overall increase of DIC suggests that the ice interior was rather heterotroph. Such sea ice system should expel CO2. Yet, strong under-saturation in CO2 and DIC depletion appeared at the ice surface, suggesting that sea ice was taking up CO2 from the atmosphere. On the whole, land fast sea ice in McMurdo Sound appears as a puzzling ecosystem. It is able to support elevated growth of autotrophic organisms at the bottom, but still appears to be heterotrophicin parallel to high remineralization, while the top of the ice appears to be rather heterotrophic but stilland able to pump CO2 from the atmosphere. [less ▲]

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See detailRepresentations about physical activity among firefighters. A study in Wallonie
Dubru, Gilles ULiege; Gribomont, Antoine; Cloes, Marc ULiege

Poster (2017, July)

Introduction To be a firefighter is a dangerous job. Many authors have found anxiety, stress, depression, sleep disorders, absenteeism and other deviant behaviors in this type of high-risk occupation ... [more ▼]

Introduction To be a firefighter is a dangerous job. Many authors have found anxiety, stress, depression, sleep disorders, absenteeism and other deviant behaviors in this type of high-risk occupation (Fullerton, Ursano & Wang, 2004 ; Carey, Al-Zaiti, Dean, Sessanna & Finnell, 2011 ; Elliot & Kuehl, 2007). Adopting an active lifestyle is therefore very important in reducing these health risks and thus maximizing the chances of staying healthy. It is for this reason that physical activity has an essential role for these people. According to this determining the place occupied by physical activity within the regional Walloon fire servicesseemed relevant. The first step should be to analyse the firefighters’ representations and lifestyle. Methods An online questionnaire (SurveyMonkey) was developed. It proposed a majority of closed-ended questions facing on age (38 ± 10.4 years), BMI (26.1 ± 3.7), gender (96.7% of men), rank, status (38.3% professional), PA representations and practices, well-being and self-esteem, job satisfaction, … We contacted one of the main firefighters association (FRCSPB) to reach a significant number of topics. 443 questionnaires were collected during a five weeks period. 394 were usable. 376 responses were needed to achieve a 95% confidence level with a 5% confidence interval based on all Belgian Firefighter. Results and discussion Results showed that firefighter showed positive representations about PA and were aware of the benefits of a regular practice on their health. Good stress management and cardiovascular endurance qualities were considerate as important in this profession. Nevertheless, subjects deplored a lack of maintenance of these qualities. The mean BMI of the subjects was above 26, indicating a slight overweight. Sedentary lifestyle, poor infrastructure, injuries due to lack of fitness allow us to suspect an insufficient training. Other factors were pointed out by the subjects (stress, absenteeism, deviant behavior…). Firefighters were generally satisfied with their job and considered that they have an acceptable quality of life. However lack of time, financial and human resources must not be a barrier to the practice of essential PA for those people who are directly related with danger. References Carey, M. G., Al-Zaiti, S. S., Dean, G. E., Sessanna, L., & Finnell, D. S. (2011). Sleep problems, depression, substance use, social bonding, and quality of live in professional firefighters. The international journal of occupational and environmental medicine, 53(8), 928-933. Doi: 10.1097/JOM.0b013e318225898f. Elliot, D. L., & Kuehl, K.S. (2007). The effects of sleep deprivation on firefighters and EMS responders: final report. International Association of fire Chiefs. Fullerton, C. S., Ursano, R.J.,& Wang, L. (2004). Acute stress disorder, posttraumatic stress disorder, and depression in disaster or rescue workers. The American Journal of Psychiatry, 161(8), 1370-76. [less ▲]

Detailed reference viewed: 37 (2 ULiège)