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See detailDetection of eye movements specific to drowsiness and their relation with subjective assessment: A cognitive ergonomic approach.
Blavier, Adelaïde ULg; Montagnino, Cédric; Wertz, Jérôme ULg et al

Poster (2014, May 27)

Drowsiness is one of the major factors explaining accidents, particularly in traffic accidents but also in work situations with serious consequences (e.g. medicine). The drowsiness may be assessed by ... [more ▼]

Drowsiness is one of the major factors explaining accidents, particularly in traffic accidents but also in work situations with serious consequences (e.g. medicine). The drowsiness may be assessed by diverse measures that vary from physiological and unconscious data (e.g. EEG) to subjective and conscious evaluation. In their daily life, people are used to evaluate their drowsiness by subjective assessment and research observes a great inter-individual variation in this evaluation. Moreover, the subjective evaluation is dependent on the situation and the risk perceived by the person (e.g., new versus usual situations, simple versus complex environments, etc.). In this theoretical context, our purpose was to investigate the links between 1) objective performance (reaction time) measured by a psychomotor vigilance task (PVT), 2) data from eye movements and 3) subjective assessment of drowsiness (measured with Karolinska Sleepiness Scale, KSS). 12 persons, aged from 20 to 56, participated individually. They were asked to respect a 60% sleep deprivation during the night before the experiment and to not drink any energy drinks the day of the experiment. The experiment was conducted between 1 and 3 PM after a heavy lunch in order to increase the circadian effect. Moreover, the temperature of the room was 25° in order to increase drowsiness. Each participant was asked to perform 4 PVT. However, although 100% of participants performed the first two PVT, only 66% were able to perform the third PVT and only 33% of participants performed the fourth and last PVT. Our results showed an effect of time on objective performance, eye movements and subjective assessment of drowsiness in PVT 1 and 2: significant increase of reaction time, increase of eye closure and perclos, reduction of pupil diameter and increase of subjective drowsiness estimation (KSS). Only the frequency and duration of blinks remained constant across time. In PVT 3 and 4, data from eye movements and objective performance (reaction time) did not vary anymore across the time. Only the subjective estimation of drowsiness (KSS) continued to increase. Furthermore, subjective estimation of drowsiness was differently correlated with eye movements and objective performance across the PVT; it was significantly correlated with reaction time (PVT 1,2,3), blink frequency and duration (PVT 1,2,3), perclos (PVT 1,2,3,4), eye closure (PVT 2,3), pupil diameter (PVT 1,2,3,4). These results are discussed and integrated in an ergonomic approach in order to analyze the links between objective performance, eye movements and subjective assessment of drowsiness. [less ▲]

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See detailJuvenile sexual offenders: Can their language tell us how their mind works?
Courtain, Audrey; Glowacz, Fabienne ULg

Poster (2014, May 27)

A significant proportion of sexual delinquency being committed by juveniles, various fields of research have studied this topic. Still, little is known about the relation between the linguistic ability ... [more ▼]

A significant proportion of sexual delinquency being committed by juveniles, various fields of research have studied this topic. Still, little is known about the relation between the linguistic ability and the cognitive functioning of this specific youth. Rooted in scientific works which have explored indirectly the point, this poster attempt to question the quality of language as a mean to find out how their mentalization occurs, but also to question how their cognitive processes (e.g. empathy) influence their language. These hypothesis are to be tested soon with a clinical sample of 47 participants and with different measures (e.a. MACI, verbal IQ, IRI). Hence, the opportunity to evaluate, among others, empathy deficits and cognitive distortions through language can be considered a promising approach allowing new perspectives for prevention, assessment and treatment of juvenile sexual offenders. [less ▲]

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See detailCorrelation and integration of the Rorschach Comprehensive System and the TCI-R questionnaire
Rentmeister, Daniel; Blavier, Adelaïde ULg; Di Piazza, Laetitia ULg et al

Poster (2014, May 27)

In this study, a dual purpose is sought. First, we statistically compare the Rorschach test interpreted by the Comprehensive System from J.E. Exner and the Temperament and Character Inventory – Revised by ... [more ▼]

In this study, a dual purpose is sought. First, we statistically compare the Rorschach test interpreted by the Comprehensive System from J.E. Exner and the Temperament and Character Inventory – Revised by R. Cloninger to highlight their differences and similarities. Second, through clinical cases, we discuss the relevance of integrating the results obtained by these two tools to establish a comprehensive and consistent psychological profile of an individual. For the statistical comparison, a sample of 27 “healthy” subjects (M = 24.37 years, SD = 2.75) was first submitted to the Rorschach test. Then, immediately after, each subject completed the TCI-R. A correlation analysis (Pearson Rho) was done between various Index and a Bonferroni adjustment was made. For the case studies, 3 subjects were recruited. Anamneses were carried out and the Rorschach test and TCI-R administered. Our first results, which are to be taken with caution because of the small number of subjects in the sample, must be qualified and discussed. From 2448 statistical comparisons, we select the most relevant and discuss different correlation results. The case studies show a concordance in social relationships and impulsivity; and show that the information peculiar to each test allows us to complete the subject’s profile. Finally, it is by addressing two different epistemologies underlying these two tests that we can best explain the psychological differences highlighted in the same individuals. We conclude by suggesting that this difference in results must certainly be understood in an integrative logic and emphasizes the complementarity between these two tests. [less ▲]

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See detailInfluence of melodic context on the perception of vocal pitch accuracy
Beeken, Manon; Morsomme, Dominique ULg; Larrouy, Pauline ULg

Poster (2014, May 27)

In order to categorize sung performances as “in tune” or “out of tune”, one can measure the deviation between the performance and the musical notation. Depending on the study, a deviation of a semitone ... [more ▼]

In order to categorize sung performances as “in tune” or “out of tune”, one can measure the deviation between the performance and the musical notation. Depending on the study, a deviation of a semitone (100 cents) or a quartertone (50 cents) is considered as “out of tune”. However, these values are arbitrary and the current study aims to define perceptual thresholds of pitch accuracy in a melodic context. For this purpose, melodic sequences were manipulated, from “in tune” (deviation of 0 cent) to “out of tune” (10 to 80 cents, in 10 cents steps). In a 2x2x2 design we systematically varied the conditions of: melodies (ascending/descending target-interval), type of errors (interval or tonality deviation), and direction of the deviation (enlargement or compression). The sequences were presented to 30 non-musicians using the method of limits procedure, in a test/retest paradigm. For each condition, they were asked to specify whether the presented singing performances were “in tune” or “out of tune”. The results showed that participants were consistent in their evaluation between the test and the retest. The pitch accuracy threshold (M = 29 cents, ES = .75) was not influenced by the melody, the type of errors, and the direction of the deviation. This study highlights the ability of non-musicians to perceive small pitch deviations (less than a quartertone) in a melodic context. This finding elucidates on the concept of pitch accuracy and therefore yields the opportunity to revise objective tools for the evaluation of singer pitch accuracy. [less ▲]

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See detailEmpathy and social relationships in suicidal adolescents
Jadin, Aurore ULg; Vermaelen, Noémie; Blavier, Adelaïde ULg

Poster (2014, May 27)

Empathy and suicide among minor people are two research topics currently very explored. Psychologists often speak about suicide because it represents the second cause of mortality among 15-24-year-old ... [more ▼]

Empathy and suicide among minor people are two research topics currently very explored. Psychologists often speak about suicide because it represents the second cause of mortality among 15-24-year-old adolescents (Delvenne, 2005). Several studies have already analyzed the links between empathy skills and some psychopathology (behavioral disorders, hyperactivity and alcoholism) but no research before this one has developed empathic abilities in suicidal adolescents. The aims of the present study were respectively to examine suicidal adolescents’ empathy skills, interpersonal capacities and to investigate the quality of their social relationships. [less ▲]

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See detailMaintaining people with multiple sclerosis at work: Validation of a questionnaire identifying their professional difficulties
Blavier, Adelaïde ULg; Monisse, Aurélie; Berkail, Radia et al

Poster (2014, May 27)

The purpose of this paper is to present the construction and validation of a questionnaire in order to evaluate the specific work difficulties and compensation mechanisms developed by multiple sclerosis ... [more ▼]

The purpose of this paper is to present the construction and validation of a questionnaire in order to evaluate the specific work difficulties and compensation mechanisms developed by multiple sclerosis patients (MS). At the moment, only few very recent questionnaires (in English) are constructed in order to highlight the link between the patients’ cognitive deficits with their professional situation (Doogan & Playford, 2014; Honan et al., 2012; McFadden et al., 2012). This lack of accurate assessment is detrimental to the patients’ job retention. Our questionnaire was constructed from review of literature and interviews of patients and experts. In order to validate our questionnaire and to test its specificity to MS patients, three groups of participants answered the questionnaire: MS patients, diabetic patients (diabetes is also a chronic illness but without any cognitive deficits contrary to MS) and a control group. We controlled the effect of anxiety and depression and we conducted a factorial analysis that showed the existence of 3 factors: the ergonomic factor (that includes cognitive difficulties and resources and adaptation process), the description of work situation and the social support. The three groups of participants obtained similar results for the work description and the social support while the group of patients with MS obtained a higher score than the other 2 groups for the ergonomic factor. These results suggest that our questionnaire is specific to the MS population and to the difficulties they specifically meet in their work situations. These results highlight new research opportunities and specific ways to increase job retention in MS population. [less ▲]

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See detailSynthesis of a glucosamine labeled amphiphilic polymer for drug delivery application
Riva, Raphaël ULg; Boyère, Cédric; Debuigne, Antoine ULg et al

Poster (2014, May 27)

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See detailLong protocols to Rorschach Comprehensive System and a posteriori reduction of number of responses: What is the impact on the clinical interpretation ?
Dufour, Cristelle; Englebert, Jérôme ULg; Blavier, Adelaïde ULg

Poster (2014, May 27)

Our study was interested in the exceptionally long protocols, superior to 55 answers, supplied by five subjects in the test of Rorschach according to the method of the Comprehensive System. In order to ... [more ▼]

Our study was interested in the exceptionally long protocols, superior to 55 answers, supplied by five subjects in the test of Rorschach according to the method of the Comprehensive System. In order to gain understanding of which attitude to adopt in front of this kind of protocol, we made a a posteriori reduction of the number of answers by basing us on the recommendations of Exner (Exner, on 2001), then a comparative study between the clinical interpretation realized with all of the answers and with only the first five answers by board. We also replicate this methodology of a posteriori decrease of the number of answers with four answers by board according to the new recommendations of the system R-PAS of Meyer and al. (Meyer and al, on 2009). The sample consists of three raw coal subjects and two psychiatric subjects. Our analysis highlighted a stability of certain variables (EB, Lambda, EA for example) but a difference in the psychological profile of the subjects after reduction. We observed a clarification of the fundamental difficulties to the psychiatric subjects and rather a neutralization of these difficulties for the raw coal subjects, with particularly defence mechanisms which seem more adapted. In our sample, the consideration of all of the answers or only of the partial protocol thus brings a difference in the clinical interpretation ; as a consequence any reduction should be made with big caution. Our study allows to discuss current practices of the test of Rorschach, by looking for a compromise between the inherent constraints to the validity of the test and the questions of the clinical complexity decrease appropriate to any initiative of standardization. [less ▲]

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See detail[18F]UCB-H AS A BRAIN SV2A RADIOTRACER: A FIRST CLINICAL TRIAL
Bahri, Mohamed Ali ULg; Bastin, Christine ULg; Aerts, Joël ULg et al

Poster (2014, May 27)

[18F]UCB-H is a fluorine-18 radiolabelled PET imaging tracer with a high affinity for the synaptic vesicle protein 2A (SV2A). This protein, involved in vesicle trafficking and widely distributed in the ... [more ▼]

[18F]UCB-H is a fluorine-18 radiolabelled PET imaging tracer with a high affinity for the synaptic vesicle protein 2A (SV2A). This protein, involved in vesicle trafficking and widely distributed in the brain, represents the binding site and the primary mechanism of the antiepileptic drug levetiracetam. Levetiracetam has recently been suggested to reduce synaptic deficits in a mouse Alzheimer’s disease model and to improve cognition in patients with amnestic mild cognitive impairment, suggesting a possible role for this protein in synaptic integrity. The objective of this study was to investigate the cerebral distribution of [18F]UCB-H in healthy human volunteers. Dynamic PET imaging of the head of four healthy volunteers was performed over 100 minutes after injection of 170.4 ± 24.9 MBq of GMP produced [18F]UCB-H. The input function was acquired by arterial blood sampling during the dynamic PET acquisition. Blood data analysis showed a consistent tracer amount in whole blood and plasma indicating a very low degree of binding of the tracer to the red blood cells. Unchanged [18F]UCB-H fraction in plasma follows a bi-exponential behavioral decrease with a starting fraction of 92% of the injected amount of the tracer, measured at 3 min post injection. This fraction decreases to about 50% at 10 min post injection. The [18F]UCB-H PET data revealed a high and rapid uptake in the grey matter structures, matching the known ubiquitous distribution of SV2A in the brain. The kinetics of the tracer in the brain was characterized by an initial high uptake phase followed by rapid washout allowing the standard compartmental modeling (1-tissue compartment, 2-tissue compartment, and Logan graphical analysis). The three models gave consistent results. The two-tissue compartment model fitted the experimental data best and provided a total distribution volume of [18F]UCB-H in the brain greater than 7 mL/cm3 and a specific distribution volume around 3 mL/cm3. Our results indicate that [18F]UCB-H is a new radiotracer for brain SV2A proteins suitable for human studies. Further studies are warranted to assess SV2A modifications in neurological pathologies such as Alzheimer’s disease. [less ▲]

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See detailAn innovative lens surface functionalization to control Posterior Capsular Opacification
Huang, Yi-Shiang ULg

Poster (2014, May 26)

Cataract is the opacity of the lens, causing impairment of vision or even blindness and the surgery is still the only available treatment today. The intraocular lens (IOL) is a polymer implant designed to ... [more ▼]

Cataract is the opacity of the lens, causing impairment of vision or even blindness and the surgery is still the only available treatment today. The intraocular lens (IOL) is a polymer implant designed to replace the natural lens in the cataract surgery. However, the bio-inert materials could not satisfy the unmet need in the secondary cataract control. Posterior capsular opacification (PCO, or Secondary Cataract), characterized by a thick and cloudy layer of lens epithelial cells (LECs), is the most common postoperative complication. For the present study, a bioactive molecule is immobilized onto the conventional acrylic hydrophilic polymer pHEMA (Poly(2-hydroxyethyl methacrylate)) using oxygen plasma treatment followed by dip-coating deposition. The RGD peptide sequence, being well-known for its ability to promote cellular attachment by binding to integrin receptors, is designed to stimulate the adhesion of LECs on the IOL. The data have shown the peptide immobilized biomaterial not only exhibits similar optical and physical properties, but also reveals enhanced bio-logical properties in cell adhesion and cell morphology maintenance. By means of surface functionalization of IOL to stimulate LECs adhesion, the secondary cataract could be controlled. [less ▲]

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See detailMultilayered chitosan-based fibers for skin regenaration applications
Croisier, Florence ULg; Detrembleur, Christophe ULg; Jérôme, Christine ULg

Poster (2014, May 26)

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This ... [more ▼]

Chitosan is a natural polymer derived from the chitin of crustacean or mushroom shells, that intrinsically presents haemostatic, mucoadhesive, antimicrobial and immunostimulant properties. This polysaccharide has shown a great potential for biomedical and pharmaceutical applications, on account of its remarkable compatibility with physiological medium. Besides, it is degraded in a physiological environment into non-toxic products, which make chitosan an outstanding candidate for short- to medium-term applications. In this respect, nanometric fibers are highly interesting as their assembly mimics the skin extracellular matrix structure. Such nanofibrous materials can be prepared by electrospinning (ESP). This technique uses a high voltage to create an electrically charged jet of polymer solution or melt which leads to fibers formation. Depending on the polymer characteristics (a.o. molecular weight, solution viscosity and conductivity) and processing conditions (electric potential, distance between syringe-capillary and collection plate, concentration, flow rate), polymer fibers ranging from nanometers to a few microns in diameter can be obtained and subsequently used as potential scaffolds, a.o. to form a temporary, artificial extracellular matrix. In the present study, electrospinning technique was combined with layer-by-layer deposition method (LBL) - a well-known method for surface coating, based on electrostatic interactions - in order to prepare multilayered chitosan-based nanofibers. The antibacterial properties of the obtained material were then assessed, and the presence of a multilayered deposit was confirmed by several techniques. The multilayered chitosan-based nanofibers produced present great prospects for the preparation of new biomedical scaffolds - such as wound dressings that could improve skin regeneration. [less ▲]

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See detailA multi-step process for an alternative wheat bran biorefinery
Aguedo, Mario ULg; Fougnies, Christian; Richel, Aurore ULg

Poster (2014, May 21)

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See detailBioactive Intraocular Lens - A Strategy to Control Secondary Cataract
Huang, Yi-Shiang ULg

Poster (2014, May 21)

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See detailMeasurement of Trace levle Dechlorane Flame Retardants in Food and Feed by GC-MS/MS
L'Homme, Benjamin ULg; Calaprice, C; Brasseur, Catherine ULg et al

Poster (2014, May 21)

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See detailTrappist Beers: Exploring the Cloak of Secrecy by GC×GC-TOFMS
Allen, C; Loughnane, C; Stefanuto, Pierre-Hugues ULg et al

Poster (2014, May 21)

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See detailFrom nets to bottom traps: is exploitation of Norway lobsters a suitable option for Corsican common spiny lobster fishermen?
Patrissi, Michela; Astrou, Adèle; Pelaprat, Corinne et al

Poster (2014, May 20)

In Corsica (NW Mediterranean), most of the fishing activity is composed of small-scale artisanal fisheries, and takes place on the western coast. The common spiny lobster (Palinurus elephas) is the main ... [more ▼]

In Corsica (NW Mediterranean), most of the fishing activity is composed of small-scale artisanal fisheries, and takes place on the western coast. The common spiny lobster (Palinurus elephas) is the main target of Corsican netters. However, its populations have been declining since the 1950's, questioning the sustainability of this activity. We therefore tried to assess whether the fishing effort, currently mostly focused on common spiny lobsters, could be moved towards other commercially-interesting deep crustaceans, such as the Norway lobster (Nephrops norvegicus), through diversification of artisanal fishing practices. With the help of local fishermen, we set up Scottish traps for Norway lobsters at depths of 300 to 400 meters, on sandy and muddy bottoms of both eastern and western coasts. Despite several tests using different baits and soak times at various depths or seasons, catches on the western coast were low. On the other hand, on the eastern coast, experimentation showed interesting yields, and large mean size (i.e. high commercial value) for both sexes. While more studies are needed to confirm these results and improve knowledge of Norway lobster stocks, trap fishing of this species on eastern coast of Corsica could be a suitable alternative for diversification of artisanal fisheries. [less ▲]

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See detailEvaluation of the aphid and aphidophagous beneficials diversity in a pea and potato association
Bosquée, Emilie ULg; Chevalier Mendes Lopes, Thomas ULg; Serteyn, Laurent et al

Poster (2014, May 20)

Crop associations can have beneficial effects on the control of insect pests. This study was conducted in the Shandong province (China) to assess the impact of potatoes and peas association on the ... [more ▼]

Crop associations can have beneficial effects on the control of insect pests. This study was conducted in the Shandong province (China) to assess the impact of potatoes and peas association on the populations of aphids and aphidophagous beneficials. Observations on potato plants and trapping were performed. During the season, the degree of infestation didn’t differ significantly between the association and the pure stand. However, the aphidophagous beneficials were more abundant in the association comparing to the pure stand. Trapping results give a more general idea about the richness and diversity of natural enemies present in the environment. Aphidius gifuensis (Ashmead) and Aphelinus abdominalis Dalman are the most abundant parasitoids. Among predators, Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) are prevalent. Few hoverflies and lacewings were trapped. Results from the observations on plants do not support the resource concentration hypothesis. However, the enemy hypothesis was confirmed for the aphidophagous beneficials. Therefore, this association can have a beneficial effect on the control of aphid populations. [less ▲]

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See detailEffects of wheat/oilseed rape and wheat/pea associations on the diversity of aphids and aphidophagous beneficials
Chevalier Mendes Lopes, Thomas ULg; Bosquée, Emilie ULg; Honba, David et al

Poster (2014, May 20)

This study was conducted in the Shandong province (China) to better characterize the effect of wheat/oilseed rape and wheat/pea associations on the populations of aphids and aphidophagous beneficials. At ... [more ▼]

This study was conducted in the Shandong province (China) to better characterize the effect of wheat/oilseed rape and wheat/pea associations on the populations of aphids and aphidophagous beneficials. At specific times of the season, the observations on wheat tillers confirm the resource concentration and the enemy hypotheses. In fact, aphids were more abundant in the pure stand of wheat, while more ladybirds were observed in the associations. As for the trapping of aphidophagous beneficials, the parasitoid species Aphidius gifuensis (Ashmead) was prevalent. Among predators, Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) were the most abundant species. Few lacewings and hoverflies were trapped. This study shows a beneficial effect of crop associations on the control of aphid populations. [less ▲]

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See detailA methodology for elicitor screening of winter wheat infected by STB and FHB
Le Mire, Géraldine ULg; SIAH, Ali; Deleu, Magali ULg et al

Poster (2014, May 20)

Un doctorat au sein de Gembloux Agro Bio-Tech (Plateforme Agriculture Is Life) vise à développer une méthode à base d'éliciteurs bio-sourcés pour protéger le blé d'hivers contre la Septoriose (STB) et la ... [more ▼]

Un doctorat au sein de Gembloux Agro Bio-Tech (Plateforme Agriculture Is Life) vise à développer une méthode à base d'éliciteurs bio-sourcés pour protéger le blé d'hivers contre la Septoriose (STB) et la Fusariose (FHB). Dans ce cadre, la mise au point d'un protocole efficace d'infection du blé est élémentaire avant de pouvoir entamer les tests de screening d'éliciteurs. Nous présentons ici une méthode d'infection du blé par Septoria tritici et les résultats de ces tests de reproduction de symptômes. [less ▲]

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See detailFive Years of aphidophagous species sampling in belgian corn
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Durieux, Delphine ULg et al

Poster (2014, May 20)

The community of aphidophagous species present in agroecosystems is disturbed since the introduction of an exotic species the Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera ... [more ▼]

The community of aphidophagous species present in agroecosystems is disturbed since the introduction of an exotic species the Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae). In this intensive agricultural area, five aphids predator species are commonly observed: three coccinellids H. axyridis, Coccinella septempunctata, Propylea quatuordecimpunctata, one hoverfly Episyrphus balteatus, and one lacewing Chrysoperla carnea. This study focuses on the occurrence of the five most abundant aphid predators and their seasonal abundance in corn. The abundance of adults and larvae of these species was evaluated over a five-year period, from 2009 to 2013. The sampling method consisted in the counting of aphids and all developmental stages of aphidophages present in quadrats of 1m² from April to November. Densities of aphid predators changed during five years studies. Since 2011, H. axyridis was the most abundant aphids predators in corn. H. axyridis numbers were found to increase over the first four inventoried year, reaching in 2012 86% for adult stage (119,7±7,8 adults/100m2) and 76% for larvae stages (242,8±14,2 larvae/100m2) of the aphid predators, while in 2009 these ratios were 14% and 23% respectively. For C. septempunctata and P. quatuordecimpunctata the population densities decrease at the end of the five years period. Population densities of C. carnea and E. balteatus were variable during the sampling period but increased in 2013. Phenology of the five studied species presents similar curves following the aphid abundance. The most abundant observed aphids were metopolophium dirhodum, rhopalosiphum padi, rhopalosiphum maidis, sitobion avenae and sitobion fragariae. H. axyridis starts reproducing after the peak in aphid population, suggesting that H. axyridis is able to complete its development by feeding on alternative prey such as larvae and pupae of conspecific and heterospecific. H. axyridis is a bivoltine species but the second generation was stop by the corn harvesting. [less ▲]

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See detailNeoadjuvant chemotherapy in breast cancer patients induces expression of tumor suppressor miR-34a
FRERES, Pierre ULg

Poster (2014, May 19)

Circulating microRNAs (miRNAs) are extensively studied in cancer as biomarkers but little is known about the influence of anti-cancer drugs on their expression. In this presentation, we describe the ... [more ▼]

Circulating microRNAs (miRNAs) are extensively studied in cancer as biomarkers but little is known about the influence of anti-cancer drugs on their expression. In this presentation, we describe the modifications of circulating miRNAs profile under neoadjuvant chemotherapy (NAC) for breast cancer. [less ▲]

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See detailJob characteristics and work engagement: multiple-group analyses of flexibility practices
Travaglianti, Fabrice ULg; De Zanet, Fabrice ULg; Vandenberghe, Christian et al

Poster (2014, May 16)

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See detailÉvaluation des effets de la formation et de la supervision de services d'aide aux familles qui accompagnent des personnes atteintes d¿une maladie de type Alzheimer
Marquet, Manon ULg; Missotten, Pierre ULg; Charlot, Valentine ULg et al

Poster (2014, May 15)

Introduction : Il est nécessaire de développer des interventions visant à aider les professionnels qui accompagnent des personnes âgées (atteintes ou non de démence). Les programmes existants sont ... [more ▼]

Introduction : Il est nécessaire de développer des interventions visant à aider les professionnels qui accompagnent des personnes âgées (atteintes ou non de démence). Les programmes existants sont néanmoins essentiellement destinés aux aidants en milieu institutionnel. Objectif : Évaluer, au niveau de différents paramètres (âgisme, connaissances sur la maladie d’Alzheimer, sentiment de compétence, satisfaction professionnelle, burnout, stratégies de coping), l’efficacité d’une intervention destinée à des aidants professionnels à domicile. Méthodologie : 17 aides familiales ont bénéficié d’une formation et de supervisions visant à améliorer leurs connaissances sur les démences et à les aider à mettre en place des stratégies de résolution de problèmes face aux difficultés rencontrées dans leur pratique, et ceci, afin de favoriser une vision moins stigmatisante de la maladie d’Alzheimer. Différents auto-questionnaires ont été complétés par les participantes avant et après l’intervention. Résultats : Les analyses statistiques indiquent que l’intervention permet de diminuer l’âgisme des aidantes, d’améliorer leurs connaissances sur la maladie d’Alzheimer, leur sentiment de compétence et leur satisfaction vis-à-vis de leurs tâches professionnelles. Conclusion : L’intervention est efficace puisqu’elle atteint ses objectifs initiaux. Ces résultats encouragent non seulement la formation des aidants professionnels à domicile mais aussi l’évaluation de l’efficacité des interventions proposées, à la fois auprès des aidants professionnels, informels et des personnes accompagnées. En effet, sur base de la littérature [Avorn & Langer, 1982 ; Coudin & Alexopoulos, 2010], nous faisons l’hypothèse que l’amélioration des connaissances et la diminution de l’âgisme réduisent le risque d’induire des comportements de dépendance chez les personnes accompagnées. Ce constat est important compte tenu du rôle des aides familiales, à savoir aider les personnes âgées (atteintes ou non de démence) qui présentent des difficultés dans la réalisation des activités (instrumentales) de la vie quotidienne afin qu’elles restent le plus longtemps possible à leur domicile. [less ▲]

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See detailSite-specific sediment characteristics impact on metal bioavailability and bioaccumulation in the Polychaete Nereis virens
Pini, Jennifer; Richir, Jonathan ULg; Watson, Gordon

Poster (2014, May 15)

The king ragworm (Nereis virens) is a cosmopolitan species of soft sediment inter-tidal communities and is known to be impacted by various pollutants. More precisely, in many coastal locations, N. virens ... [more ▼]

The king ragworm (Nereis virens) is a cosmopolitan species of soft sediment inter-tidal communities and is known to be impacted by various pollutants. More precisely, in many coastal locations, N. virens is exposed to a range of metals including copper and zinc which are known to be highly toxic. However, the relationships between metal bioavailable concentrations in the sediment, the pore water and the tissues of N. virens have not yet been investigated in this is ecologically and commercially important species. Hence, to investigate these relationships, sediment, pore water and associated worms samples were collected from seven sites along the English Channel coast of the UK, chosen for their different pollution histories. The BCR three-step sequential extraction procedure was used to assess the bioavailable metal concentrations in the sediment, in conjunction to standard extraction techniques to determine the tissue and the pore water concentrations. All samples were measured by Atomic Absorption Spectroscopy (FAAS). Results from correlation analyses showed that site specificities and metal pollution histories along with sediment characteristics (organic content and particle size) were important factors regarding the bioavailability of copper and zinc to worms. Indeed, sediment organic content and grain size were positively correlated to metal bioavailable concentrations in the sediment. Strong correlations were found between copper bioavailability in the sediment and in the pore water and between zinc concentrations in the pore water and in N. virens. These results showed that zinc from the pore water was more readily bioavailable to N. virens than copper. However, our data suggested that N. virens accumulated metals regardless of the pollution history/level of the sites. In addition, our data showed that metal concentrations in N. virens were lower than those found in other closely related polychaetes such as N. diversicolor. This suggests that N. virens, unlike other polychaetes, is well adapted to highly contaminated sites by the possible use of specific metal regulation mechanisms, which would require further analysis. [less ▲]

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See detailImpact des stéréotypes liés au vieillissement et à la maladie d’Alzheimer sur le vécu des aidants familiaux
Bottard, Aude; Adam, Stéphane ULg; Gilles, Christian et al

Poster (2014, May 15)

INTRODUCTION Dans notre société, les personnes âgées atteintes de la maladie d’Alzheimer sont les cibles à la fois de stéréotypes liés à l’âge, mais aussi de stéréotypes liés à cette maladie (Sartorius ... [more ▼]

INTRODUCTION Dans notre société, les personnes âgées atteintes de la maladie d’Alzheimer sont les cibles à la fois de stéréotypes liés à l’âge, mais aussi de stéréotypes liés à cette maladie (Sartorius, 2003). Leur aidant familial est également souvent touché par l’image négative de la pathologie que les stéréotypes imputent à leur proche (Werner, 2008). Aussi, cette recherche a pour objectif de mieux connaître le point de vue d’aidants familiaux sur la maladie d’Alzheimer et le vieillissement, pour mieux appréhender les implications de ces représentations sur leur vécu. METHODE Un ensemble de questionnaires, dont des mesures de la vision du vieillissement (FSA-R ; Boudjemadi & Gana, 2009) et de la maladie d’Alzheimer (FS-ADS ; Werner et al., 2011), ont été administrés à 38 aidants familiaux dont le proche est atteint de la maladie d’Alzheimer ou d’une démence apparentée. RESULTATS Les analyses statistiques montrent que les individus qui présentent une vision négative du vieillissement sont ceux qui sont aidants depuis plus longtemps. Par ailleurs, les personnes qui ont un vécu négatif de la maladie (qui ont honte de leur proche ou qui sentent que les autres ont honte de lui), sont ceux qui se sentent moins compétents en tant qu’aidant. De même, le fait d’avoir peur de son proche, est associé à un épuisement physique et émotionnel, ainsi qu’à une humeur triste et anxieuse. CONCLUSION Les stéréotypes liés à l’âge et à la maladie péjorent plusieurs éléments du vécu de l’aidant (santé, sentiment de compétence, expérience en tant qu’aidant.). En conséquence, il importe d’encourager la déconstruction des stéréotypes et idées reçues véhiculés par la société, notamment par le biais de programmes d’aide aux aidants [less ▲]

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See detailQualitative and quantitative analyses of a pharmaceutical formulation produced by hot melt extrusion using Raman spectroscopy
Netchacovitch, Lauranne ULg; De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg et al

Poster (2014, May 15)

In the pharmaceutical industry, Hot Melt Extrusion (HME) is a recent technique used to integrate poor water soluble drugs in pharmaceutical formulations. Indeed, more and more active pharmaceutical ... [more ▼]

In the pharmaceutical industry, Hot Melt Extrusion (HME) is a recent technique used to integrate poor water soluble drugs in pharmaceutical formulations. Indeed, more and more active pharmaceutical ingredients (API) belong to the Biopharmaceutical Classification System (BCS) II and IV. Their integration in pharmaceutical solid forms is a big deal. HME processes increase the bioavailability and the solubility of those API by encompassing them in a polymeric carrier and by forming solid dispersions [1]. Moreover, in 2004, the FDA’s guidance initiative promoted the usefulness of Process Analytical Technology (PAT) tools when developing a manufacturing process. Vibrational spectroscopy is an appropriate PAT tool to analyze extrudates [2 – 3]. In this case, Raman spectroscopy, which belongs to vibrational spectroscopy, was used to analyze itraconazole extrudates qualitatively and quantitatively. During HME, the main objective is to develop solid dispersions by converting a crystalline API in an amorphous one, in order to improve its solubility and bioavailability [4]. According to Raman spectra, it is possible to identify the polymorphic form of the components in the extrudates by integrating or rationing the Raman bands that are characteristic of the API or by calculating the width at half intensity of some bands [5]. After determining the polymorphic form of the API, a quantitative method was developed in order to measure the ratio between the API and the polymer. Finally, chemical imaging was performed on extrudates to identify the distribution of the homogeneity of the API inside the polymer [6]. In conclusion, Raman spectroscopy is an appropriate tool to follow an extrusion process. By qualitative and quantitative analyses it is possible to determine the composition, the polymorphic form, the homogeneity, and the concentration of pharmaceutical matrices according to Raman fingerprint. References: [1] S. Shah et. al., Int J Pharm 453 (2013) 233 – 252. [2] L. Saerens et. al., Anal Chem 85 (2013) 5420 – 5429. [3] T. De Beer et. al., J Pharm Biomed Anal 48 (2008) 772 – 779. [4] A. Sarode et. al., Eur J Pharm Sci 48 (2013) 371 – 384. [5] E. Widjaja et. al., Eur J Pharm Sci 42 (2011) 45 – 54. [6] J. M. Amigo, Anal Bioanal Chem 398 (2010) 93 – 109. [less ▲]

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See detailDevelopment of an analytical method to detect simultaneously bisphenols using a multiplexed surface-enhanced Raman scattering quantitative approach
De Bleye, Charlotte ULg; Dumont, Elodie ULg; Sacre, Pierre-Yves ULg et al

Poster (2014, May 15)

Over the last decade, bisphenol A (BPA) attracted a lot of attention. This molecule, commonly used as a precursor to produce epoxy-resin and plastics, is an endocrine disruptor presenting an estrogenic ... [more ▼]

Over the last decade, bisphenol A (BPA) attracted a lot of attention. This molecule, commonly used as a precursor to produce epoxy-resin and plastics, is an endocrine disruptor presenting an estrogenic activity [1]. Despite its health toxicity, BPA is present in a broad variety of consumer goods released from plastic bottles and packaging for example. Since the discovery of its adverse health effect of BPA, the manufacturers tend to use structural analogues of BPA such as BPS, BPF and BPB to produce plastic materials [2]. However, the health safety of these molecules is still not demonstrated. Currently, bisphenols are actively researched and quantified using solid phase extraction and chromatography techniques which are time and solvents consuming. Therefore, it could be very interesting to quantify simultaneously bisphenols using a fast and “green” technique. Surface-enhanced Raman scattering (SERS) exalts dramatically the Raman scattering of molecules adsorbed or very closed to metallic surface enabling to detect very low amounts of analytes while keeping the structural information obtained from the spectrum which is very interesting to consider multiplexed analyses [3-4]. Moreover, SERS, which is a solvent free and fast acquisition technique, is an attractive tool in “Green Chemistry” [5]. In this context, the development of a multiplexed quantitative approach to detect bisphenol was considered. Silver nanoparticles (AgNps) were selected as SERS substrate and their functionalization was investigated taking into account the weak affinity of phenolic molecules for gold and silver surface [6]. Pyridine was selected as surface modifier and allowed to attract bisphenols around metallic surface thanks to hydrophobic interaction and hydrogen bonds [7]. Afterwards, the SERS samples preparation was optimized playing on the concentrations of pyridine and aggregating agent used to get the nanoparticles closer to each other which promotes the SERS effect. Tap water samples were spiked with different concentration of BPA from 5 ppb to 1000 ppb and analyzed using the optimized SERS sample preparation. A good linearity of the response was observed and a calibration curve with coefficient of determination (R2) of 0.9922 was obtained by plotting the intensity of a principal band of BPA versus the concentration. This last step was repeated using BPB as analyte and a calibration curve with a R2 of 0.9991 was obtained for the same range of concentration using a specific band intensity of BPB. Finally, tap water samples were spiked with different concentrations of BPA and BPB simultaneously and analyzed using SERS and it was possible to detect selectively the two molecules thanks to specific bands and a good linearity of the response was observed for both. To conclude, promising results were obtained which pave the way to “green” multiplexed quantitative analyses of very low concentrated analytes using SERS. References: [1] J.-H. Kang et al., Toxicology 226 (2006) 79-89 [2] C. Liao et al., J. Agric. Food Chem. 61 (2013) 4655-4662 [3] K. Kneipp et al., Chem. Rev. 99 (1999) 2957-2975. [4] R.F. Aroca et al., Adv.Colloid Interface Sci. 116 (2005) 45-61. [5] C. De Bleye et al., Talanta 116 (2013) 899-905. [6] X.X. Han et al., Anal. Chem., 83 (2011) 8582-8588. [7] B. San Vicente et al., Anal. Bioanal. Chem. 380 (2004) 115-122. [less ▲]

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See detailThe effects of flowering signals on root architecture in Arabidopsis
Mathieu, Laura ULg; Lobet, Guillaume ULg; Périlleux, Claire ULg

Poster (2014, May 15)

Roots are responsible for water and nutrient uptake and hence are critical to sustain the whole plant life cycle. This study aims at characterizing how root development is affected by flowering. We ... [more ▼]

Roots are responsible for water and nutrient uptake and hence are critical to sustain the whole plant life cycle. This study aims at characterizing how root development is affected by flowering. We therefore focused on the effects of systemic signals produced in the leaves at floral transition, namely the proteins FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF), on root growth and branching. These proteins act in the shoot apical meristem as potent promoters of flowering [1] but their effects in other parts of the plant are still unknown. [less ▲]

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See detailHow to build a biological linker dedicated to the engineering of novel drug delivery systems
Crasson, Oscar ULg; Galleni, Moreno ULg; Parente, Raffaella et al

Poster (2014, May 15)

Nowadays, chemical linkages are widely used in industry to coat bioactive molecules on biocompatible supports. However, some issues inherent to the linkage procedure remain. For example, chemical ... [more ▼]

Nowadays, chemical linkages are widely used in industry to coat bioactive molecules on biocompatible supports. However, some issues inherent to the linkage procedure remain. For example, chemical reactions often damage the structure and therefore the activity of the immobilized molecules. In this work, we propose the use of a human chitin binding domain (ChBD) to immobilized bioactive molecules on a polysaccharide-based surface. ChBD belongs to the human chitinase called chitotriosidase and was shown to interact specifically with chitin and chitooligomers. Using molecular biology and protein engineering, we have developped of a new biological tool based on the hybrid protein technology. In this technology, we can create chimeric proteins that have the ability to bind polysaccharidic supports thanks to their binding domain that can be covalently linked with any bioactive molecule that can confer prophylactic or therapeutic activities. In this study, we have successfully shown that we can use the chitin binding domain (ChBD) from the human chitotriosidase as a biological linker dedicated to the engineering of novel drug delivery systems containing chitin or other analogous polysaccharides. [less ▲]

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See detailDiscrimination of two geographically distinct populations of sea bass Dicentrarchus labrax, using stable isotopic signatures of mercury (δ202Hg, Δ199Hg)
Cransveld, Alice ULg; Amouroux, David; Koutrakis, Emmanuil et al

Poster (2014, May 15)

Despite the reduction of mercury (Hg) emissions in Europe in the last decades, Hg emissions are increasing worldwide and concentrations found in some marine predators remain high. This raises questions on ... [more ▼]

Despite the reduction of mercury (Hg) emissions in Europe in the last decades, Hg emissions are increasing worldwide and concentrations found in some marine predators remain high. This raises questions on mercury's biogeochemical cycle at both local and global scale. In the present work, we investigate the possibility to use mercury isotopic signature in fish as a tool to discriminate different polluted areas and potential pollution sources. Indeed, Hg can exhibit both mass-dependent (MDF) and mass-independent fractionation (MIF). While MDF may occur during biological cycling inter alia and could be used to understand bioaccumulation processes, MIF provides a unique fingerprint of specific chemical pathways, such as photochemical transformations. In this context, information provided by Hg isotopes would help to improve environmental management strategies. A preliminary set of four and ten juvenile common sea bass, Dicentrarchus labrax were collected from the North Sea and the Aegean Sea respectively. T-Hg was analysed by direct mercury analyser (DMA), speciation by gas chromatography inductively coupled plasma mass spectrometer (GC-ICP-MS) and Hg isotope analysis were performed using cold vapour generation with multicollector ICP-MS. Total Hg concentrations in all tissues were higher in individuals from the North Sea ( Hgtot muscle=1,14 ± 0,48 mg.kg-1 dw) than from Greece (0,60 ± 0,06 mg.kg-1 dw). Speciation analysis shows that MeHg is the predominant form of Hg in muscle (89% MeHg on average) but not in liver (51% MeHg on average). Isotopic mass dependent values (δ202Hg) values were always higher in muscle than in liver and related to Hg species distribution. For mass independent isotopic signature, sea bass from the Aegean Sea had a systematically higher Δ199Hg value than individuals from the North sea (e.g.: 0,56 ± 0,05‰ and 0,32 ± 0,06‰ respectively in muscle). While mass dependent isotopic signature probably reflects some internal Hg metabolism, mass independent isotopic signature seems definitely site dependent. Such isotopic discrimination might be in agreement with difference in both mercury sources and cycling in the North and Aegean Seas. These preliminary results indicate that Hg isotopes may thus help to discriminate fish from different areas. This promising outcome must be further confirmed by extending the number of individuals and locations to be investigated. [less ▲]

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See detailTest of humic substances on in vitro roots initiation using isolated leaves of woody species
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

Poster (2014, May 15)

Arise from the chemical and biological degradation of plant and animal residues and from the synthetic activities of microorganisms in the soil, humic substances (HS) are natural heterogeneous aromatic ... [more ▼]

Arise from the chemical and biological degradation of plant and animal residues and from the synthetic activities of microorganisms in the soil, humic substances (HS) are natural heterogeneous aromatic and organic compounds. These substances are chemically complex with no clearly defined chemical structure, although generalized models have been proposed and they can be divided into fractions of humic acids, fulvic acids and humins depending on their solubility in water as a function of the pH. The stimulation of plant growth and development by HS are the activities that have attracted the attention of many scientists. They influence plant productivity directly by the stimulation of biochemical and metabolic processes or indirectly through the modification of soil characteristics and microflora activities. All together, these properties mainly affect root architecture. By inducing root hairs proliferation, differentiating root cells and enhancing lateral root emergence, an increase of the total root biomass is observed. Experiments targeting the rooting stages in absence of interferences were conducted in vitro using HS extracted from landfill leachate and a stable commercial formulation (“Humifirst” from TRADECORP company: 12% humic acid and fulvic acid 3%) issued from leonardite. Shoots and leaves explants of silver birch (Betula pendula Roth) and black alder (Alnus glutinosa L. Gaertn) vitro-plants were treated with 10 ppm leachate HS and 100 ppm Humifirst HS for 5 days during the rooting induction/initiation phase. The treated explants were then transferred into elongation medium containing only nitrate calcium for 4 weeks. The results show that application of HS during the root induction/initiation phase did not significantly influence root growth of both species in comparison with control explants. [less ▲]

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See detailPerception of climate change and adaptation strategies in the drylands of West Africa : A review
De Longueville, Florence ULg; Ozer, Pierre ULg; Vigil, Sara ULg

Poster (2014, May 15)

The perception of climate change has become an exiting new field of research explored in recent years. This is a response from the international scientific community to the conclusions of the 4th report ... [more ▼]

The perception of climate change has become an exiting new field of research explored in recent years. This is a response from the international scientific community to the conclusions of the 4th report of the Intergovernmental Panel on Climate Change which had underlined serious deficiencies in this field, particularly in West Africa where population is largely dependent of rain-fed agriculture. Yet, a global survey of the literature since 2000 shows that most references on this topic were published over the last five years. [...] [less ▲]

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See detailMaize cold tolerance : in search of relevant parameters for discriminating genotypes
Riva-Roveda, Laetitia ULg; Escale, Brigitte; Giauffret, Catherine et al

Poster (2014, May 15)

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See detailCadmium and mercury sediment pollution along the British coastline of the Channel: A first spatio-temporal integrated approach
Richir, Jonathan ULg; Pini, Jennifer; Watson, Gordon

Poster (2014, May 15)

Cadmium (Cd) and Mercury (Hg) are 2 widespread trace metals occurring in both terrestrial and marine environments. They play no physiological role and are often toxic even at low concentrations and were ... [more ▼]

Cadmium (Cd) and Mercury (Hg) are 2 widespread trace metals occurring in both terrestrial and marine environments. They play no physiological role and are often toxic even at low concentrations and were therefore identified as priority hazardous substances under the European Water Framework Directive and included in the European List I of Dangerous Substances. Marine sediments are considered as the ultimate sink of non-degradable metals, where they can accumulate in considerable quantities. The UK coastline has been polluted with metals for decades or even centuries and, although many studies have monitored sediment metal loads in specific locations, there has been no recent systematic monitoring approach along the whole of the south coast. In addition, few reliable data exist on decadal temporal trends for most sediment pollutants. Focusing on Cd and Hg, we have investigated their spatial and temporal distribution in sediment samples collected from 42 sites along the English coast of the Channel from 1990 to 2010. From a temporal point of view, data analyses showed that mean Cd levels in sediments significantly decreased by 59 % during that period of time, from 0.78 to 0.32 mg kgDW-1 of sediments. This reflects a decrease globally to below the 0.70 mg kgDW-1 Cd Threshold Effect Level (TEL). Adverse effects caused by Cd on the biota should, therefore, be rare. Spatially, Cd displayed an east-to-west increase of its levels, with several western sites with Cd still above the TEL. Regarding Hg, sediment levels did not significantly change from 1990 to 2010, remaining approximately twice as high as the corresponding 0.13 mg kgDW-1 Hg TEL. Hg also displayed an east-to-west increase of its levels, whilst 55 % of monitored sites had a mean sediment Hg concentration in average 3.5 times higher than the TEL. Despite regulations, non-degradable metals such as Cd and Hg that have accumulated in sediments over considerable periods of time may consequently still pose threats to aquatic organisms, highlighting the need for continued extensive spatially and temporally integrated monitoring approaches. [less ▲]

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See detailIdentifying root-secreted proteases in Arabidopsis thaliana: an Activity-Based Protein Profiling approach.
Lallemand, Jérôme ULg; Périlleux, Claire ULg; Tocquin, Pierre ULg

Poster (2014, May 15)

Proteases are involved in many physiological processes during the whole life of the plant, such as embryonic development, defense against pathogens, nutrition or mycorrhiza creation. However, the ... [more ▼]

Proteases are involved in many physiological processes during the whole life of the plant, such as embryonic development, defense against pathogens, nutrition or mycorrhiza creation. However, the functions of many of the 800 proteases of Arabidopsis thaliana still remain unknown. Besides discovering new functions, studying proteases can also result in improving plant biotechnology. Indeed, plants can be used as hosts for recombinant protein production. Some proteins of interest require to be secreted in order to fold properly, but production yields are limited due to their degradation by endogenous extracellular proteases. The aim of our study is to identify active root-secreted proteases of Arabidopsis thaliana. Their activity was first analyzed by in vitro incubation with a target protein (BSA) at different values of pH and in the presence of proteases inhibitors. This analysis identified serine proteases as the major protease class involved in BSA degradation. Then, an Activity-Based Protein Profiling approach led to the labeling of two active serine proteases in the root-secreted sample. Finally, a further step towards the identification by mass spectrometry, based on affinity purification, was developed. [less ▲]

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See detailBelgian Grasshoppers: A Nutritious Food Source
Paul, Aman ULg; Frederich, Michel ULg; Blecker, Christophe ULg et al

Poster (2014, May 14)

Rapid urbanization and rising economies are creating shifts in the composition of global food demand, so it is necessary to explore new sources of food with better nutritional profile. Among the ... [more ▼]

Rapid urbanization and rising economies are creating shifts in the composition of global food demand, so it is necessary to explore new sources of food with better nutritional profile. Among the alternative food that exists are the grasshoppers, about 80 species of which are consumed worldwide. Grasshoppers are not only rich source of proteins and lipids but also some important minor component like vitamins and minerals. Edible species of grasshopper in Belgium were identified and attempts were made for the lab rearing of meadow grasshopper (Chorthippus parallelus). The lipids as well as protein contents of meadow grasshopper (Chorthippus parallelus) & long winged conehead (Conocephalus discolor) were investigated. The fatty acid compositions of these two species were determined by gas chromatography. Some of the physicochemical properties of the lipids extracted were also analyzed. These two grasshopper species could be really nutritious source of food. [less ▲]

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See detailAdaptation to beach erosion in the African coastal cities: from the procrastination by the Government to the forced migration of the most precarious population. The case of Cotonou, Benin
Ozer, Pierre ULg; De Longueville, Florence ULg; Hountondji, Yvon Carmen et al

Poster (2014, May 14)

This analysis shows that the current impacts of geomorphological changes result mainly from the sum of human activities. However, it is necessary to consider the risk of coastal erosion and forced ... [more ▼]

This analysis shows that the current impacts of geomorphological changes result mainly from the sum of human activities. However, it is necessary to consider the risk of coastal erosion and forced migration (of mostly poor people) in the coming decades with the amplification of sea level rise due to global warming. [less ▲]

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See detailImproving soil conservation through an adapted tillage: experience from the cultivation of potatoes in Bamiléké's hills of Cameroon
Djoukeng, Henri Grisseur ULg; Tankou, Christopher Mubeteneh; Degré, Aurore ULg

Poster (2014, May 14)

The western highlands of Cameroon are an area where the population density and growth rates are the highest in the country. In this region, the main activity is agriculture, and rivers are the main source ... [more ▼]

The western highlands of Cameroon are an area where the population density and growth rates are the highest in the country. In this region, the main activity is agriculture, and rivers are the main source of safe drinking water. Lacks of arable land and especially against very complex land tenure, farmers are increasingly exploiting the hills (9-30 % slope) for the production of vegetable crops, mainly potatoes. The methods of preparation of soil found there are ridging in the direction of the slope and the culture dish. These practices promote erosion by runoff, generally compromises the multiple functions of agriculture, and in particular the environmental function (soil degradation, pollution and silting up of rivers) and the production function (incomes). In order to ensure soil stability and maintain good water quality of rivers, we put it up a new way of preparing the ground: the tied ridging. To quantify the effectiveness of the latter, we conducted tests of erosion by runoff with Wischmeier’s plots on the most exploited slopes, namely 11% and 29%. The run-off water and sediments were collected per plot and per block after every rain. The first analyses show that there is a significant difference between the culture dish or ridging along the slope and tied ridging. The major constraint of this practice lies in the increase of about 17% of the labor. Tied ridging reported losses in average land 16% lower compared with the ridging in the direction of the slope and 22% compared with the culture dish, he also presented higher yields of 65 % compared with the culture dish and the ridging in the direction of the slope. Despite the hardship, the test results have convinced the participants and some curious farmers which have adopted the technology during the next growing season. [less ▲]

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See detailMicroplastics contamination in two planktivorous and commercial fish species
Collard, France ULg; Parmentier, Eric ULg; Das, Krishna ULg

Poster (2014, May 13)

Plastic pollution is a huge environmental concern and affects each marine ecosystem. Plastics are produced by millions of tonnes each year in the world and finally accumulate in oceans. They adsorb many ... [more ▼]

Plastic pollution is a huge environmental concern and affects each marine ecosystem. Plastics are produced by millions of tonnes each year in the world and finally accumulate in oceans. They adsorb many persistent organic pollutants, cause external and internal wounds and provoke blockage of the digestive tract of marine mammals, birds and turtles. Plastics can also threaten marine organisms of small size class in the same way by fragmenting in smaller parts that result in microplastics of less than five millimetres. These microplastics are of the same order of magnitude than plankton and can thus be ingested by filter-feeders, suspension-filters and planktivorous organisms such as fish. Few studies deal with microplastics ingestion by fish and even less by commercial fish species. The herring (Clupea harengus) and the sardine (Sardina pilchardus) were respectively the third and the eighth most caught fish species in the world in 2009. We focused our research on these two species which are of economic importance. We sampled around thirty individuals of each species in the Channel and in the North Sea in January 2013. The stomach contents were digested by sodium hypochlorite and then analyzed. Microplastics were characterized by size, colour and shape. The results of these analyses will highlight the need for studies about microplastics ingestion by planktivorous species. [less ▲]

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See detailFully implicit integration scheme for the GTN model
Guzmán Inostroza, Carlos Felipe ULg; Habraken, Anne ULg

Poster (2014, May 12)

Fully integration scheme for the GTN model, applied to some shear extensions. The model also comprises Hill type anisotropy and mixed (Isotropic/kinematic) hardening. The consistent tangent matrix is also ... [more ▼]

Fully integration scheme for the GTN model, applied to some shear extensions. The model also comprises Hill type anisotropy and mixed (Isotropic/kinematic) hardening. The consistent tangent matrix is also calculated. [less ▲]

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See detailModeling of a glass mineral wool process in view of Life Cycle Analysis
Gerbinet, Saïcha ULg; Léonard, Angélique ULg; Briard, Vincent

Poster (2014, May 12)

In line with the growing concern about the environmental impact of materials in the building sector, Knauf Insulation, a glass wool producer, is performing environmental impact assessment of its products ... [more ▼]

In line with the growing concern about the environmental impact of materials in the building sector, Knauf Insulation, a glass wool producer, is performing environmental impact assessment of its products through LCA. Knauf Insulation has several glass wool factories in Europe that produce various products, and for a specific market, the same product can be produced in several factories. As the plants that produce glass wool work with similar pathways, a generic model for LCA usable for every plant and every glass wool product has been designed. Moreover, combination of different factories is also possible. The general principle of glass wool production is the following: the raw materials, sand, limestone, soda ash, borax, sodium carbonate, as well as recycled off-cuts from the production process, are weighed and mixed. Knauf Insulation also uses a large amount of recycled glass (cullet). The mix is sent to a furnace at high temperature (1350°C). The melted material is then fiberized and the binder is added, a process called forming. Knauf Insulation uses a special binder with ECOSE Technology, a new and formaldehyde-free binder. The wool fibers are collected, by suction, on a conveyor belt, and the mattress then goes through the curing oven. For some product a facing is added. Finally the product is compressed and packed. The model, implemented in GaBi 6, is made as generic as possible by including, for each step, all the raw materials that can be used in one of the factories as well as all the energy sources. Parameters allow to define the amount of each raw material consumed, therefore the model can be adapted to any factory simply by setting these parameters accordingly. This also simplifies the data collection, since the template is the same for all the factories, it can be supported by data collection tools already existing. A part of the model is dedicated to weighting between factories, so a combination of factories can also be studied. The model can also be adapted to almost all Knauf Insulation products by using parameters where necessary: for example, several products have different binder contents, so a parameter defines the amount of binder. As some materials can be recycled between several parts of the process, special attention has been paid to recycling loops inside the model. The model is flexible enough to be used for Environmental Product Declaration (EPD) as well as for Eco-Design purposes. [less ▲]

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See detailModeling using the SWAT model of water flow and transport in suspension in the watershed of the valley of Wadi El-Hachem
Tadrist, Nassima ULg; DEBAUCHE, OLIVIER ULg; Degré, Aurore ULg et al

Poster (2014, May 12)

In the Maghreb, dams regularly suffer from excessive siltation due to erosion problems present in the catchment areas. The origins of this erosion are multiple: land use, deforestation, land affectation ... [more ▼]

In the Maghreb, dams regularly suffer from excessive siltation due to erosion problems present in the catchment areas. The origins of this erosion are multiple: land use, deforestation, land affectation , ... Hydrology coupled with geographic information systems allows using distributed and physically based models to predict the evolution of siltation of dams. The application of these models in Algeria will finally predict the impact of anti-erosion measures, land use patterns on the siltation of dams accurately estimating the amount of sediment produced by erosion runoff and the degree of filling. A methodology based on the map data (digital terrain model, map soil science, geology map and mapping of land use) and daily meteorological data (temperature, relative humidity, wind speed, solar radiation) is being development and testing of the dam Bourkourdane. The SWAT model is used to predict the amount of sediment accumulating in the dam, the water flow rate inbound, outbound, and the volumes of water and sediment stored in the dam. Adequate management of releases, coupled with better management of erosion upstream of the dam will extend the life of dams Algerians. Especially for Boukourdane, improved management of releases is paramount to improve groundwater recharge, fight against the intrusion of marine waters and prevent the accumulation of fine particles that reduce soil permeability. [less ▲]

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See detailTrace element bioaccumulation in rope-grown Mytilus galloprovincialis: knowledge update
Richir, Jonathan ULg; Gobert, Sylvie ULg

Poster (2014, May 12)

Numerous trace elements (TEs) can be considered as potential pollutants of the environment, their mining productions and industrial uses increasing worldwide. Their monitoring can be achieved through the ... [more ▼]

Numerous trace elements (TEs) can be considered as potential pollutants of the environment, their mining productions and industrial uses increasing worldwide. Their monitoring can be achieved through the use of bioindicator species, such as the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1819). That species has been widely used to monitor the chemical pollution of coastal ecosystems by Cr, Ni, Cu, Zn, Cd, Pb, As, Ag and V. Conversely, environmental levels of Be, Al, Fe, Mn, Co, Se, Mo, Sn, Sb and Bi have been little or not monitored so far in mussel watch programs. Bioaccumulation processes of these 19 TEs in rope-grown M. galloprovincialis purchased from a salt pond with good chemical water quality were thus investigated in the present study. Mussels efficiently accumulated the 19 studied TEs. Bioaccumulation processes were driven by numerous mutually dependent biological parameters such as the mussel size and flesh weight, the sex and the reproductive status and the body compartment considered. TE bioaccumulation was a power function of the mussel soft body dry weight; total contents linearly increased with the shell length. Small-size mussels overall concentrated more TEs, with a high interindividual variability, consequently influencing the modelling of their bioaccumulation in the whole rope population. Although a large range of rope-grown M. galloprovincialis sizes can be used for monitoring purposes, one will thus take care not to use extreme size individuals. The influence of gametogenesis in determining female body higher TE concentrations prior to spawning could not be neglected and varied depending on the element. TEs were preferentially accumulated in the hepatopancreas, except for Zn, Se, Cd and Mo, more concentrated in gills. Gametogenesis did not influence TE distribution between body compartments, but likely diluted their concentrations as a direct consequence of massive reproductive tissue production. So, results from the present study underlined the potential use of M. galloprovincialis in the biomonitoring of numerous little studied TEs and gave some insights into the decisive role played by some relevant biological parameters in bioaccumulation processes of the 19 investigated TEs in rope-grown mussels. [less ▲]

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See detailA clinical lab experience with an automated HIV Antigen/Antibody (Ag/Ab) combined assay
HUYNEN, Pascale ULg; TOUSSAINT, Françoise ULg; GERARD, Christiane ULg et al

Poster (2014, May 11)

OBJECTIVES: To describe the diagnostic performance of a new fourth-generation HIV Ag/Ab chemiluminescent immunoassay, available on the new LIAISON® XL analyser, in a clinical setting. METHODS: Through ... [more ▼]

OBJECTIVES: To describe the diagnostic performance of a new fourth-generation HIV Ag/Ab chemiluminescent immunoassay, available on the new LIAISON® XL analyser, in a clinical setting. METHODS: Through February 2012-October 2013, 12,438 samples of serum, received at our laboratory for screening for HIV infection were routinely tested with LIAISON® XL Murex HIV Ab/Ag assay (HIV-XL), which employs HIV-1, HIV-1 group O, and HIV-2 antigens and anti-p24 monoclonal antibodies in two coupled reagent cartridges, providing information of the overall Ab/Ag reactivity and detail of the specific reactivity for anti-HIV/HIV p24 antigen. Each serum with positive result or with negative result displaying a value close to the cut-off were sent to the regional AIDS-Reference Laboratory (RefLab) to perform confirmatory assays (PCR, Immunoblot). A previous verification of the HIV-XL demonstrated 100% sensitivity with a challenge panel of hundred positive sera provided by the RefLab. Performed external quality control was from United-Kingdom National External Quality Assessment Service (NEQAS). RESULTS: Out of the clinical samples, 12,312 non-reactive samples (including 6 negative results displaying a value close to the cut-off further confirmed true HIV negative), 64 Ab HIV reactive samples (all confirmed HIV-1 positive by immunoblot), including 4 samples reactive also for Ag HIV (confirmed positive by Ag assay/PCR), 42 Ab HIV reactive samples tested negative by immunoblot, and 20 Ag HIV reactive samples tested negative by the kit used for the Ag p24 detection in our HIV Reference Lab, have been found. All the 43 NEQAS specimens tested, 16 reactive and 27 non-reactive, were correctly classified. These results, considered all together, provide a calculated positive predictive value of 57.5% with an estimated specificity of 99.5% (with 95% confidence interval of 99.36-99.62%), and a calculated negative predictive value of 100% with an estimated sensitivity of 100.0% (with 95% confidence interval of 95.49-100%). CONCLUSIONS: In our experience HIV-XL showed excellent performance associated to all the advantages of a fully automated/random access instrument. [less ▲]

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See detailModeling information sharing in animal health surveillance with social network analysis
Delabouglise, Alexis; Dao Thi, Hiep; Nguyen Tien, Thanh et al

Poster (2014, May 08)

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See detailCOMPARISON AND MOLECULAR CHARACTERIZATION OF ANIMAL AND HUMAN CLOSTRIDIUM DIFFICILE STRAINS
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Korsak Koulagenko, Nicolas ULg et al

Poster (2014, May 07)

The main objective of this study was to characterize and compare animal and human C. difficile strains with respect to the PCR-ribotype and the antibiotic resistance. Multilocus sequence typing analysis ... [more ▼]

The main objective of this study was to characterize and compare animal and human C. difficile strains with respect to the PCR-ribotype and the antibiotic resistance. Multilocus sequence typing analysis (MLST) was performed in order to study clonal relationships of the isolates. [less ▲]

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See detailStudy of the microbial flora of steak tartare by metagenomic approach
Korsak Koulagenko, Nicolas ULg; Delhalle, Laurent ULg; Nezer, Carine et al

Poster (2014, May 06)

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See detailHas oxygen depletion an impact on nutrients and macrofauna in a highly dynamic macrophytodetritus accumulation?
Remy, François ULg; Mascart, Thibaud ULg; Dauby, Patrick ULg et al

Poster (2014, May 06)

Posidonia oceanica is an endemic Mediterranean highly productive seagrass. Depending on the ability of the primary consumers to digest it alive, a generally important part of its foliar primary production ... [more ▼]

Posidonia oceanica is an endemic Mediterranean highly productive seagrass. Depending on the ability of the primary consumers to digest it alive, a generally important part of its foliar primary production falls in autumn, to decay inside the meadow or to be exported to sand patches to form “exported litter accumulations”. These accumulations are highly dynamic depending on hydrodynamics and seafloor geomorphology. Literature says that low O2 conditions might occur inside litter accumulations, but the annual oxygen dynamics or its impact on the litter-associated macrofauna has never been measured. We focused on 2 exported litter accumulations in Calvi Bay (Corsica), during 2 years for a total of 8 seasons. For each season, we collected water samples (n=6) from 3 different strata: Water Column (WC), Water Just Above the litter (WJA) and Water Inside the litter (WI). Oxygen was measured for each replicate using a Winkler-based automated routine for oxygen concentration measurements on micro-volumes. At the same time, nutrients concentrations (PO4, NH4, and NO2+NO3) were measured in WC, WJA and WI, but also in the Interstitial Water (IW) using a spectrophotometric continuous flow analyzer (adapted for low nutrients level in an oligotrophic environment). In parallel, macrofaunal (size >500µm) samples (n=3-6) were also collected, counted and identified to the specific level. Our results show significant differences between O2 concentrations/saturation from WI and the two other strata. Significant differences were detected between seasons, sites and years for WI which is the only stratum where really low O2 conditions can be observed. Significant differences were also detected between seasons for both WC and WJA but no differences between sites and years. On the other hand no significant differences were detected between WC and WJA. A similar observation was made for the nutrients at the annual, seasonal and spatial level. Moreover differences are also observed between the nutrients themselves. Our data shows no correlation between WI O2 concentrations and saturation, and global macrofauna abundance or biodiversity. Results are more contrasting at an individual specific level for the 4 most dominant species. For two amphipod species, Gammarella fucicola (55% of the global abundance) and Gammarus aequicauda, no significant correlations were detected between their abundance and O2. For the leptostracan species, Nebalia strausi, a significant negative correlation with O2 concentration was detected. For the last amphipod species, Melita hergensis, a significant positive correlation was observed. Our analyses also show significant correlations between WI O2 concentration/saturation, and WI / IW nutrients concentration. To conclude, this work shows that WI is a very particular and dynamic environment considering O2 concentration and saturation. Low O2 conditions can be observed in WI but never in WC or WJA showing that internal processes and relations with the sediment determine the O2 dynamics in WI and showing a potential “barrier” effect between WI and WC. Moreover O2 dynamics and its consequences may play a role in the nutrients dynamics and cycles. It is clear that faunal responses to low O2 conditions are not identifiable at a global community level. At a specific level, we show a more complex situation: some species do not seem to be impacted by low O2 conditions, but some present a significant positive, or a significant negative response. This shows the existence and complexity of species-dependent low O2 tolerance/adaptation, and the importance of a specific level data analyses to detect responses of dominant litter associated macro- invertebrates to O2 concentration and saturation variations. [less ▲]

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See detailHypoxia in macrophytodetritus accumulation: Species specific harpacticoid copepod adaptation?
Mascart, Thibaud ULg; De Troch, Marleen; Gobert, Sylvie ULg et al

Poster (2014, May 05)

Mediterranean Posidonia oceanica seagrass meadows generate high primary production and support large biodiversity of associated fauna and flora. The majority of the foliar material falls on the ... [more ▼]

Mediterranean Posidonia oceanica seagrass meadows generate high primary production and support large biodiversity of associated fauna and flora. The majority of the foliar material falls on the unvegetated sea floor during the autumnal leaf senescence, fuelling the detrital food web. Whilst laying on the sea floor the freshly formed macrophytodetritus pile up into accumulations according to the local hydrodynamics and seafloor geomorphology. In these litter accumulations, harpacticoid copepods (Crustacea, Copepoda) are the main meiofaunal players (metazoans in the size range of 38µm – 1mm) and show a high specific diversity. They are primarily grazers, but their high specific diversity suggests that they occupy also a large variety of trophic niches. This large morphological and trophic diversity can partly be promoted by the complexity of the phytodetritus in seagrass accumulations. On the other hand, macrophytodetritus degradation and flux of reduced compounds from the sediments is responsible for oxygen consumption inside the accumulation of seagrass litter. Therefore, concentration of oxygen inside the accumulation is very variable and often under the concentration observed in the water column just above the litter. Frequently, oxygen levels reach very low values. The present study aims to link the oxygen variability inside the accumulation to the densities of the five most dominant harpacticoid copepods found living in the P. oceanica litter. Standardized samples were collected seasonally in two contrasting sites of the Calvi Bay (Corsica) during one year. Our results showed no correlation between the oxygen concentrations and harpacticoid community diversity or their total abundances. The five most dominant species showed divergent results, but none had a clear correlation with the oxygen concentration. This contrasts with observation done for sediment meiofaunal community where most harpacticoid copepods are sensitive to oxygen level and where nematodes often dominate the community. This could be explained by their high mobility and the patchiness and variability of the oxygen concentrations present in the accumulations. Harpacticoid copepods, whilst being sensitive to hypoxia and anoxia developed a strategy to live in this fast oxygen changing environment. To conclude, our results underline the importance of species-specific analysis of correlation data. Especially in complex and dynamic environments where a variety of potential trophic niches are present and species competition is very likely to occur. The overall abundance pattern and diversity of the copepod community showed no relation to the oxygen concentration while the most abundant copepod species did not responded to fluctuating oxygen concentrations. [less ▲]

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See detailCorrelation between the acoustic noise field measured in a Posidonia oceanica bed and the photosynthetic activity
Felisberto, P; Zabel, F; Rodriguez, O et al

Poster (2014, May 05)

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See detailRates of microbial sulfur oxidation in low oxygen environments
Crowe, SA; Canfield, DE; Sturm, A et al

Poster (2014, May 05)

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See detailAmmonia Oxidising Archaea in the OMZ of a freshwater African Lake
Lliros, M; Ingeoglu, O; Garcia-Armisen, T et al

Poster (2014, May 05)

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See detailAnaerobic methane oxidation in two tropical freshwater systems
Roland, Fleur ULg; Darchambeau, François ULg; Crowe, SA et al

Poster (2014, May 05)

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See detailHeroin-assisted treatment showed better efficacy than methadone
ANSSEAU, Marc ULg; Demaret, Isabelle ULg

Poster (2014, May 04)

Background: A fraction of patients receiving methadone treatment pursues their use of street heroin. In Switzerland, a new treatment with prescribed diacetylmorphine (pharmaceutical heroin) was developed ... [more ▼]

Background: A fraction of patients receiving methadone treatment pursues their use of street heroin. In Switzerland, a new treatment with prescribed diacetylmorphine (pharmaceutical heroin) was developed to help these heroin addicts resistant to methadone treatment to decrease their street heroin use. In this heroin-assisted treatment (HAT), diacetylmorphine is prescribed to severe heroin user and diacetylmorphine is administered by patients under the supervision of nurses in a specific centre. Six randomised controlled trials compared HAT to methadone treatment: in Switzerland, The Netherlands, Spain, Germany, Canada and United- Kingdom. HAT showed better efficacy than methadone. Patients used less street heroin, their health improved and their criminal behaviour decreased. A new trial assessed in Belgium the feasibility and efficacy of this treatment compared to methadone treatment. Methods: The TADAM (Treatment Assisted by Diacetylmorphine) was an open label randomised controlled trial developed on the Swiss model of HAT developed in 1994. Main inclusion criteria were 5 years of heroin addiction, (almost) daily use of street heroin and a previous attempt of methadone treatment. As in the Dutch experiment, patients could choose to inhale or to inject diacetylmorphine in the new HAT centre. HAT was stopped after 12 months and the best available treatment was offered to the patient. The research team assessed subjects every three months with standardised questionnaires (EuropASI, MAP-HSS, SCL-90-R) and questions on involvement in a criminal milieu. We completed our reported data with toxicological analysis and criminal proceedings. Results: 74 subjects were randomised in the trial: 36 in the experimental group and 38 in the control group. According to the primary efficacy criterion, the experimental group counted at least 30% more responders than the control group after 3 months (p<0.05), 6 months (p<0.05) and 9 months (p<0.01). At the 12 month assessment, the number of responders was still higher in the experimental group but the difference (11%) was no more significant (p=0.35). At the 12 month assessment, the condition of patients in the experimental group worsened compared to the 9 month assessment. This effect was not seen in the control group were patients could continue their methadone treatment after the 12 months. Conclusion: As in other countries, HAT is an effective treatment for severe heroin addicts resistant to methadone treatment. However, a predetermined duration of 12 month counteracts the efficacy of this treatment. [less ▲]

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See detailTropospheric jet stream as a source of traveling ionospheric disturbances observed by GPS
Wautelet, Gilles ULg; Warnant, René ULg

Poster (2014, May 02)

The integrity and the reliability of real-time precise positioning applications with Global Positioning System (GPS) are affected by the ionospheric variability with time and space. As a consequence ... [more ▼]

The integrity and the reliability of real-time precise positioning applications with Global Positioning System (GPS) are affected by the ionospheric variability with time and space. As a consequence, scientific community aims at describing, explaining and forecasting the occurrence and the amplitude of ionospheric irregularities observed by GPS. The use of the geometric-free combination of GPS dual frequency signals allows to retrieve the Total Electron Content (TEC) along the satellite-to-receiver path, which is the basic trans-ionospheric observable. Based on L1/L2 GPS phase measurements collected at a given station, the TEC high-frequency variability is isolated. A climatological study performed over 10 years in Western Europe shows that TEC irregularities are mostly observed daytime during quiet geomagnetic background in autumn and winter and correspond to classical Medium-Scale Traveling Ionospheric Disturbances (MSTIDs). The latter are generally understood as the ionospheric signature of Atmospheric Gravity Waves (AGWs), either generated in situ (solar terminator) or in the lower atmosphere and propagating upward. Because of its associated strong wind shears, the tropospheric jetstream, occurring mainly during autumn and winter months, constitutes an ideal candidate for AGW generation. This paper analyzes the spatial correlation between the presence of both MSTIDs and strong jetstream over Western Europe. This correlation is positive when the ionospheric pierce point of the satellite is located above regions of interest where wind shears are very large. In practice, we have selected regions for which wind speed is larger than 50 m/s. In addition, the propagation of AGWs up to the ionospheric layer is taken into account by assuming horizontal and vertical velocities of 200 and 50 m/s respectively. It comes that the region of interest of the correlation study is computed using an isotropic slant propagation of the AGW, which is supposed to be generated at a tropospheric level.Based on 30s GPS data collected over several stations in Belgium and on European Centre for Medium-Range Weather Forecasts (ECMWF) wind velocity maps, the correlation study covers a period ranging from January 2002 to December 2011. Preliminary results based on a limited number of cases show that large amplitude MSTIDs are generally observed during periods of strong wind speeds at an altitude corresponding to a pressure level of 250hPa (about 10 km). [less ▲]

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See detailEOF analysis of long-term reconstructed AVHRR Pathfinder SST in the South China Sea
Huynh, Thi Hong Ngu ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2014, May 02)

Sea surface temperature (SST) is one of the key variables often used to investigate ocean dynamics, ocean-atmosphere interaction, and climate change. For recent decades, the AVHRR Pathfinder SST, measured ... [more ▼]

Sea surface temperature (SST) is one of the key variables often used to investigate ocean dynamics, ocean-atmosphere interaction, and climate change. For recent decades, the AVHRR Pathfinder SST, measured by infrared sensors, has been widely used because of its high resolution and long time-series. The disadvantage of the AVHRR Pathfinder SST is high percentage of missing data due to cloud coverage. This becomes more serious in the South China Sea (SCS) because it is located in the tropical region, frequently covered by clouds. In this study, we used the Data INterpolating Empirical Orthogonal Functions (DINEOF) method to reconstruct daily night-time 4 km AVHRR Pathfinder SST spanning from 1989 to 2009 for the whole SCS. In order to better understand the spatial and temporal variability of the SCS SST, an EOF analysis of the reconstructed field is performed in association with surface wind. The first SST mode, accounting for 69% of the variance, presents the cooling (warming) of the basin due to the solar inclination through seasons, water exchange, topography, and monsoon-induced cyclonic circulation. The second SST mode, explaining 24.8% of the variance, shows the advection of cold and warm water from two opposite directions along the southwest-northeast diagonal of the basin. The second SST mode is affected by the atmospheric anticyclone (cyclone) located over the Philippine Sea. Comparing both SST modes with Nino3.0 index, it shows that the interannual variability of the SCS SST is influenced by the moderate and strong ENSO events with a lag of 5-6 months. Moreover, the analysis of the high-resolution reconstructed dataset reveals some oceanic features that could not be captured in previous EOF analyses. [less ▲]

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See detailEffect of metal nanoparticles encapsulated in porous silica on the biphenyl biodegradation by Rhodococcus erythropolis T902.1
Wannoussa, Wissal ULg; Hiligsmann, Serge ULg; Heinrichs, Benoît ULg et al

Poster (2014, May 01)

Biphenyl is stable and highly hydrophobic, thus having a low availability for degrading microorganisms, which need an aqueous environment for their growth. As a consequence, bioremediation processes are ... [more ▼]

Biphenyl is stable and highly hydrophobic, thus having a low availability for degrading microorganisms, which need an aqueous environment for their growth. As a consequence, bioremediation processes are very limited. However, a few studies showed that using a low metal concentration accelerates the biodegradation of pollutants (Yeom and Yoo1997). Nanoparticles are considered as a new generation of compounds to improve environmental remediation and biological processes (Beckers et al. 2013; Zhang 2003). This paper investigated the enhancement effect of nanometre-sized metallic Cu, Ag, Pd or Co, on the biphenyl biodegradation by Rhodococcus erythropolis T902.1. Have been synthesized by a sol–gel process (Lambert et al. 2004). These nanoparticles (NP) of about 2–3 nm were encapsulated in porous silica (SiO2) and were added at low concentration (10-4M) in the M284 minimal medium with 500 ppm biphenyl as source of carbon and energy. The cultures containing Pd or Co produced 30% more biphenyl degraded with a higher Rhodococcus growth than those without NP (positive control) or with silica particles only. On the contrary, the presence of 10-4 M Cu or Ag nanoparticles showed an inhibitory effect on bacterial growth and biphenyl degradation compared to the positive. [less ▲]

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See detailBias correction using data assimilation: Application on the Lorenz ’95 and NEMO-LIM models.
Canter, Martin ULg; Barth, Alexander ULg

Poster (2014, May 01)

Data assimilation has been used for decades in fields like engineering or signal processing to improve forecast models. Ensemble Kalman filters and other sequential data assimilation methods are examples ... [more ▼]

Data assimilation has been used for decades in fields like engineering or signal processing to improve forecast models. Ensemble Kalman filters and other sequential data assimilation methods are examples of developments which reduce the uncertainty of the model by taking observations into account. The widespread interest in addressing systematic forecast model errors only arose when the advances in modelling, data assimilation and computational power had reduced random errors to the point of commensurability with systematic errors, also known as bias. We present here a new method of bias correction using data assimilation. This method is based on the stochastic forcing of a model to correct bias. First, through a preliminary run, we estimate the bias of the model and its possible sources. Then, we establish a forcing term which is directly added inside the model’s equations. We create an ensemble of runs and consider the forcing term as a control variable during the assimilation of observations. We then use this analysed forcing term to correct the bias of the model. Since the forcing is added inside the model, it acts as a source term, unlike external forcings such as wind. This procedure has been developed and successfully tested with a twin experiment on a Lorenz 95 model. Indeed, we were able to estimate and recover an artificial bias that had been added into the model. This bias had a spatial structure and was constant through time. The mean and behaviour of the corrected model corresponded to those the reference model. It is currently being applied and tested on the sea ice ocean NEMO LIM model, which is used in the PredAntar project. NEMO LIM is a global and low resolution (2 degrees) coupled model (hydrodynamic model and sea ice model) with long time steps allowing simulations over several decades. Due to its low resolution, the model is subject to bias in area where strong currents are present. We aim at correcting this bias by using perturbed current fields from higher resolution models and randomly generated perturbations. [less ▲]

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See detailEVALUATION OF THE RAPID DETECTION OF ST-17 AND ST-1 GROUP B STREPTOCOCCI USING A MICROFLEX MALDI-TOF MS (BRUKER)
MEEX, Cécile ULg; SACHELI, Rosalie ULg; DESCY, Julie ULg et al

Poster (2014, May)

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to ... [more ▼]

Objectives Clearly associated to neonatal meningitis, Group B streptococci (GBS) classified as sequence type-17 (ST-17) are defined as the “highly virulent” clone amongst GBS. The aim of this study was to evaluate an easy and rapid method, recently described to detect ST-17 and ST-1 GBS, based on distinguishing peak-shifts present on the protein spectrum of these 2 sequence types, using a Microflex (Bruker) matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS). Methods This study was performed on 67 multi locus sequence typed (MLST) GBS originated from the Belgian and Czech National Reference Centers, including 18 ST-17 and 16 ST-1. After culture on blood agar, an ethanol/formic acid extraction was performed on each strain. Each extract was spotted once on a target plate, overlaid with 1 µl alpha-cyano-4-hydroxycinnamic acid matrix and further analysed by a Microflex MALDI-TOF MS. One spectrum per isolate was recorded, 240 laser shots being recorded for each spectrum. The spectra were further analysed using a Bruker prototype software, and 2 logarithmic values, one for ST-17 and one for ST-1, calculated from the intensities of the present and absent peaks, were obtained for each strain. If >0, this value indicated the presence of the specific sequence type. In a second step, the test was repeated on each strain with discordant result when compared with MLST. Results Compared with MLST method, the first analysis of the strains gave poor results, leading to very low sensitivities (77.8% for ST-17 and 50% for ST-1) but rather good specificities (85.7% for ST-17 and 98.0% for ST-1). After repeating the analysis on the strains with discordant result, sensitivity, 100% and 93.8%, and specificity, 87.8% and 98.0%, for ST-17 and ST-1 respectively were highly improved. Conclusion Since ST-17 and ST-1 GBS both show distinguishing peak-shifts on their protein spectrum, as described by Lartigue et al., the distinction of these 2 sequence types is now possible by MALDI-TOF MS. To our knowledge, this study is the first describing this application on a Microflex MS using a software to classify the strains. The observed results are promising but, given to the variability of the logarithmic value given by the software, the need to perform several measures on a same strain seems to be essential. After optimization of the analysis procedure, this rapid, easy and cheap method could be used to precociously detect ST-17 among GBS isolated from prenatal screenings, allowing a better follow up of the colonized mothers and a closer monitoring of their newborns. We would like to thank the Bruker Company which allowed us to evaluate the prototype software they have developed. [less ▲]

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See detailQuantitative multi-parameter mapping in parkinson’s disease: preliminary results
Rouillard, Maud ULg; D'Ostilio, Kevin ULg; Albinet, Cedric et al

Poster (2014, May)

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See detailEvent justice and social entity justice: A cross-lagged analysis
Marzucco, Laurence ULg; Hansez, Isabelle ULg

Poster (2014, May)

ABSTRACT This study aimed to examine the directionality of the relationship between event justice and social entity justice. Using two samples and a cross-lagged panel analysis with two measurement times ... [more ▼]

ABSTRACT This study aimed to examine the directionality of the relationship between event justice and social entity justice. Using two samples and a cross-lagged panel analysis with two measurement times, social entity justice was found to predict event justice (procedural, distributive, interpersonal and informational justice event), supporting the top-down process. PRESS PARAGRAPH The life of organizations is punctuated by a wide range of managerial decisions (e.g., hiring, performance appraisal or organizational change). Facing such events, employees evaluate the fairness of the situation experienced. However, little is known about how employees develop justice judgments. This study shows that employees’ fairness perceptions about their organization influence their interpretation of the fairness of subsequent events involving the organization. Building and fostering a climate of justice is therefore of primary importance for organizations, since global fairness perceptions about the organization may help employees to perceive a specific event, such as a managerial decision, as being fair. [less ▲]

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See detailOutplacement adequacy and benefits: The mediating role of retrospective justice
Marzucco, Laurence ULg; Hansez, Isabelle ULg

Poster (2014, May)

ABSTRACT We examined the relationships between perceived outplacement adequacy, retrospective justice and outplacement benefits for redundant employees using outplacement services. Based on an ... [more ▼]

ABSTRACT We examined the relationships between perceived outplacement adequacy, retrospective justice and outplacement benefits for redundant employees using outplacement services. Based on an outplacement experience perceived as adequate, victims formed retrospective justice perceptions about their former organization that in turn, impacted their negative emotions, well-being, future perspectives and job search. PRESS PARAGRAPH The increasing prevalence of organizational downsizing has been matched by growth in the provision of outplacement services over the last decade. Despite this rapid growth and ongoing need for outplacement services, little is yet known about the perceived adequacy and the benefits of these services for redundant employees using them. Our results indicated that an outplacement experience perceived as adequate for redundant employees retrospectively fosters their impressions of justice about the dismissing organization; this leads in turn to satisfactory benefits for them: reduction of negative emotions, enhancement of their perceived well-being, future perspectives, and job-seeking activities. [less ▲]

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See detailFemmes auteures d’homicide en détention : des liens d’attachement aux ruptures de liens
Glowacz, Fabienne ULg; Bernardello, Sandrine; Résimont, Isabelle

Poster (2014, May)

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See detailWork-home interference and well-being: A cross-lagged analysis.
Babic, Audrey ULg; Stinglhamber, Florence; Barbier, Marie ULg et al

Poster (2014, May)

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See detailLong-term evolution and seasonal modulation above Jungfraujoch (46.5°N, 8.0°E): Optimisation of the retrieval strategy, comparison with model and independant observations
Bader, Whitney ULg; Stavrakou, J; Muller, J-F et al

Poster (2014, May)

Methanol (CH3OH) is the second most abundant organic compound in the Earth’s atmosphere after methane. In this work, we present the first long-term time series of methanol total, lower tropospheric and ... [more ▼]

Methanol (CH3OH) is the second most abundant organic compound in the Earth’s atmosphere after methane. In this work, we present the first long-term time series of methanol total, lower tropospheric and upper tropospheric-lower stratospheric partial columns derived from the analysis of high resolution Fourier transform infrared solar spectra recorded at the Jungfraujoch station (46.5°N, 3580 m a.s.l.). The retrieval of methanol is very challenging due to strong absorptions of ozone in the region of the selected 8 band of CH3OH. Two wide spectral intervals have been defined and adjusted in order to maximize the information content. Methanol does not exhibit a significant trend over the 1995-2012 time period, but a strong seasonal modulation characterized by maximum values and variability in June-July, minimum columns in winter and a peak-to-peak amplitude of 130 %. In situ measurements performed at the Jungfraujoch and ACE-FTS occultations give similar results for the methanol seasonal variation. The total and lower tropospheric columns are also compared with IMAGESv2 model simulations. There is no systematic bias between the observations and IMAGESv2 but the model underestimates the peak-to-peak amplitude of the seasonal modulations. [less ▲]

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See detailDioxins and PCBs in eel and Chinese mitten crabs in the Rhine-Meuse estuary
van Hattum, B; Nijssen, P; Focant, Jean-François ULg et al

Poster (2014, May)

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See detailSEDIMENTS OXIDATION BY SEAGRASSES: INFLUENCE ON THE S CYCLE IN POSIDONIA OCEANICA (L.) DELILE INTERMATTES DYNAMIC
Abadie, Arnaud ULg; Lejeune, Pierre; Pergent, Gérard et al

Poster (2014, May)

The Mediterranean seagrass Posidonia oceanica forms meadows and develops a complex of rhizomes, roots and sediment which is called “matte”. P. oceanica meadows show discontinuity patterns in the form of ... [more ▼]

The Mediterranean seagrass Posidonia oceanica forms meadows and develops a complex of rhizomes, roots and sediment which is called “matte”. P. oceanica meadows show discontinuity patterns in the form of sand or dead matte (matte without living shoots) patches, called “intermattes”, which can have natural or anthropogenic origins. Mechanical processes (e. g. currents, anchoring) can initiate intermattes formation but their dynamic after creation seems to be linked with the sediment chemistry, especially with S cycle. P. oceanica plays an important role in controlling coastal belowground biogeochemistry, in particular by oxidizing sediments through the release of O2 by roots. This process allows creating more suitable condition for plant growth and colonization. The lack of H2S oxidation in SO4 2- can lead to limitation of the plant development or its regression. In order to investigate the effect of oxidation condition in sediments on intermattes dynamic and the neighboring meadow, we initiate, in December 2013, a study on six intermattes (three natural, three anthropogenic) at different depths in Calvi Bay, in Corsica (France). We hypothesize that redox potential and H2S concentration in sediments play an important role in the regression of P. oceanica meadows, particularly after a mechanical anthropic impact like anchoring. It also may be possible that two different kinds of processes are involved for each type of intermatte. Regular samplings throughout two years are planed with the aim of evaluating the seasonal variations of physicochemical parameters. [less ▲]

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See detailCorrelation between composition and cold stability of palm liquid fractions.
Danthine, Sabine ULg; Lefebure, Emilie ULg; Dijkmans, Peggy et al

Poster (2014, May)

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See detailL'enfant placé au carrefour de ses appartenances
Aubinet, Suzanne ULg

Poster (2014, May)

Michard (2009) nous dit de la loyauté qu’elle est l’armature de l’appartenance et à Neuburger de souligner qu’en plus d’être un socle sur lequel l’identité se structure, l’appartenance contient et limite ... [more ▼]

Michard (2009) nous dit de la loyauté qu’elle est l’armature de l’appartenance et à Neuburger de souligner qu’en plus d’être un socle sur lequel l’identité se structure, l’appartenance contient et limite les effets des relations (2003). Pour Onnis (2008) c’est dans la dialectique entre identité et appartenance que naît l’individualité qui, elle, se réalise dans la relation (Dolto, 1988). Plymackers (2006) insiste sur les notions d’espaces où se nouent ces relations mais aussi de temps où ces dernières se jouent. L’enfant, par des mouvements dialectiques d’allées et venues avec son/ses système(s) d’appartenances tisse pas à pas les prémices de son identité. Ses relations structurent et étayent ce processus et la temporalité constitue le squelette de celui-ci. Mais qu’en est-il de ces enfants placés, maltraités, déplacés ? Qu’en est-il si le conflit ou le clivage de loyauté entre famille(s) et institution(s) rend l’étayage de l’appartenance ardu ? Qu’en est-il si la discontinuité des espaces et des relations prédomine et si le processus d’individuation est suspendu par une séparation arbitraire dictée par un autrui qui « n’appartient pas » ? L’analyse clinique de cas d’enfants aux vécus chaotiques rythmés par de nombreux placements nous permet de décortiquer les particularités de ce processus complexifié par ces ruptures d’espaces, ces loyautés morcelées, ces appartenances diffuses et d’en cerner les impacts. [less ▲]

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See detailSeeking for causes of recent methane increase: comparison between GEOS-Chem tagged simulations and FTIR column measurements above Jungfraujoch
Bader, Whitney ULg; Bovy, Benoît ULg; Wecht, K et al

Poster (2014, May)

Atmospheric CH4 reached 260% of the pre-industrial level (~700 ppb) due to increased emissions from anthropogenic sources. Globally averaged CH4 reached a new high of 1819 ± 1 ppb in 2012, an increase of ... [more ▼]

Atmospheric CH4 reached 260% of the pre-industrial level (~700 ppb) due to increased emissions from anthropogenic sources. Globally averaged CH4 reached a new high of 1819 ± 1 ppb in 2012, an increase of 6 ppb with respect to the previous year (WMO, Greenhouse gas Bulletin N.9, 2013). CH4 above Jungfraujoch increases at 0.53±0.19%/year during the late 90s to stabilize and reach a non significant trend from 2000 to 2005. Since 2006, atmospheric methane has been continuously increasing with a rate of 0.19±0.05 %/year. The attribution of this increase to any CH4 source is difficult since the current network is insufficient to characterize emissions by region and source process, emphasizing the need for source-tagged model simulations as it should provide us information on processes causing the increase of atmospheric methane since 2005/2006. [less ▲]

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