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See detailRole for PARs and thrombin-GPIbα interaction in thrombin induced procoagulant platelet formation
Bourcy, Morgane ULiege; Wang, Samuel; Pasalic, Leonardo et al

Poster (2017, December 11)

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See detailTrophic ecology of icefishes (Notothenioidei, Perciformes) in a context of climate change
Peignot, Quentin; Frederich, Bruno ULiege; Lepoint, Gilles ULiege et al

Poster (2017, November 23)

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to ... [more ▼]

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to adapt their ecological habits to their new life conditions. Currently, biological traits of many Antarctic species are still unknown and we need such information to assess their capacity to adapt to natural and anthropogenic perturbations. Notothenioidei (Perciformes), or icefishes, are endemic to the Southern Ocean and they are an important trophic component of these marine ecosystems. Here we used stable isotopes ratios of carbon (δ13C) and nitrogen (δ15N) of two well represented genera of icefishes (Trematomus and Lepidonotothen) sampled in 2002/2004 and/or in 2015/2016 to better understand their resources partitioning and the influence of environmental changes on their trophic ecology. Isotopic niches (which are a proxy of the realized ecological niches) were modeled using the SIBER (Stable Isotope Bayesian Ellipses in R) R-package. Our results revealed a great variation in isotopic compositions among icefishes, suggesting that they exploit a wide array of resources. The overlap between the isotopic niches of Trematomus and Lepidonotothen genera in 2002/2004 highlights a possible past competition for resources. Moreover, temporal evolution of niches suggests taxon-specific ecological plasticity in response to variation in environmental parameters and/or in prey availability. An increase in resources partitioning between the two genera was observed over the studied period. In the future, it would be interesting to use mixing models including isotopic composition of potential preys to identify possible changes in their trophic ecology. [less ▲]

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See detailMorphological diversification of the endemic Antarctic fishes Trematominae (Notothenioidei, Teleostei)
Frederich, Bruno ULiege; Heindler, Franz Maximilian; Christiansen, Henrik et al

Poster (2017, November 23)

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with ... [more ▼]

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with emphasis on the tempo of diversification, Antarctic notothenioids represent one of the very few examples of adaptive radiation in marine fishes. Time-calibrated phylogenies suggest that the diversification of most speciose notothenioid lineages occured between 20 and 15 Ma. The subfamily Trematominae is one of those diverse subclades showing a large range of ecological niches. In the present study, we aim to describe the evolutionary history of Trematomus species. By combining a consensus time-tree and a geometric morphometric dataset, we first illustrate their pattern of cephalic shape diversification in a phylomorphospace and we infer the morphology of their common ancestor. We also explore whether the cephalic shape data shows a phylogenetic signal, which is defined as the statistical dependence among species trait values due to their phylogenetic relatedness. The combination of phylogenetic signal test and exploration of the phylomorphospace allows us to assess whether Trematomus rapidly diverged in various adaptive zones as expected under a classic scenario of adaptive radiation. [less ▲]

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See detailEffects of fluid preservation on sea star stable isotope compositions: How useful can museum collections be for trophic ecology studies?
Le Bourg, Baptiste ULiege; Lepoint, Gilles ULiege; Michel, Loïc

Poster (2017, November 23)

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or ... [more ▼]

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or frozen before subsequent analysis. However, museum samples, or samples not initially collected for SIA are often fixed in preservative solutions. These preservation methods may alter stable isotope ratios in animal tissues, and those effects should be quantified. Here, we investigated long-term effects (one year) of four preservation methods (freezing, alcohol, formaldehyde, drying) on δ13C and δ15N values of sea stars. We show that only formaldehyde preservation significantly affects δ13C values. However, after being altered, the mean δ13C value remained stable over time, suggesting that a correction factor may be used regardless of preservation time. δ15N values change across time for all methods except freezing but this may be the result of a higher intra-individual variability of δ15N values. The influence of preservation methods on estimation of isotopic niches size (parameterised as standard ellipse area) was also investigated. Bayesian estimations of standard ellipse areas (SEAB) of preserved samples were not different from those of fresh samples except for the samples preserved 6 months in alcohol. These preliminary results suggest that samples stored during one year in preservative fluids may be used for SIA in order to investigate the trophic ecology of sea stars. Future results will also help to determine whether the findings can be generalised to sea stars preserved for a longer time, such as those in museum collections. [less ▲]

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See detailA forward model for electrical conduction in soil-root continuum: a virtual rhizotron study
Rao, Sathyanarayan; Meunier, Félicien; Ehosioke, Solomon ULiege et al

Poster (2017, November 23)

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than ... [more ▼]

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than soil. We suggest that ERT data taken in agricultural fields is impacted by plant roots and might contaminate estimates of soil water content based on bare soil petrophysical relations. To understand how do roots impact electrical current flow and thus ERT data, a numerical electrical model was coupled with a mechanistic maize-soil water flow model. All the maize roots with a radius larger than 0.05 cm were explicitly accounted for in the finite element mesh and associated to their specific electrical properties. Root growth and water uptake processes continuously affected the EC contrast between soil and root. We demonstrated that high contrats between root and soil EC lead to errors in the estimation of soil water content, which could be disminished by using an appropriate biopedophysical correction term. The effective EC (bulk properties) of the medium computed using simulated plate electrodes at rhizotron boundaries reveal directional anisotropy induced by root processes and is more pronounced in sand medium when compared to loam. The percentage change in bulk EC due to change in direction (𝐸𝐸𝐸𝐸ℎ𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜 .vs. 𝐸𝐸𝐸𝐸𝑣𝑣𝑣𝑣𝑣 𝑣 𝑣𝑣𝑣𝑣𝑣 𝑣 ) starts at ~30 % in sand and ~3 % in loam when root is young and increases upto ~500% in sand and ~20% in loam at day 22 when root is three weeks old. Directions in which there is more anisotropy contains more information on the root processes and hence they can be used as prior information for ERT injection scheme to retrieve better information. [less ▲]

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See detailFirm Performance and Multi-Scale Territorial Resources in Wallonia: Towards a Model using Business Accounting?
Wilmotte, Pierre-François ULiege; Halleux, Jean-Marie ULiege

Poster (2017, November 17)

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness ... [more ▼]

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness of the (Walloon, Belgian or European) firms. For instance, in Wallonia, one of the key action of the Marshall Plan (i.e. the main regional strategy in the domain of economic development) aims to “mobilize the territory” (in French, « mobilisation du territoire à destination du développement économique »). In this perspective, our objective is to quantify and analyse the territorial resources that impact the performance of Walloon or Belgian firms, with the final aim to advice the policy makers and to improve the related public policies. This topic is related to questions such as: Does a better accessibility of economic estates improve the performance of firms? Are cultural amenities (in order to attract talents) and land prices (in order to reduce their costs) strategic competitive advantages for firms? The poster describes some methodological issues of (in general) the PhD research and (particularly) one part of the research, concerning a multivariate statistics analysis of business accounting. [less ▲]

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See detailRapid changes in preoptic estradiol concentration during male sexual behavior
de Bournonville, Marie-Pierre ULiege; de Bournonville, Catherine; Ball, Gregory et al

Poster (2017, November 11)

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis ... [more ▼]

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis (neuroestrogens) are critical for the regulation of different functions including the control of male sexual behavior. Classically, E2 acts through effects initiated in the nucleus to regulate male sexual function. Along with these long-term effects, E2 also acts rapidly (within minutes) via membrane-initiated events. These effects are thought to depend on short-term variations in the local production of estrogens, through rapid fluctuations of the enzymatic activity of brain aromatase. In Japanese quail, rapid modulations of brain aromatase activity (AA) have been reported after sexual interactions or exposure to an acute stress. These changes take place mainly in the medial preoptic nucleus (POM), a sexually differentiated structure that plays a key role in the control of male sexual behavior and where aromatase is densely expressed. Yet, it has recently been shown that, in the short term, AA does not always reflect local E2 concentration. This study was designed to determine by in vivo microdialysis whether local E2 concentrations fluctuate during sexual interactions and test whether these changes parallel the decrease in AA observed ex vivo after copulation. We first conducted a series of experiments to validate the microdialysis and E2 assay. When dialysis probes were placed in successive baths containing known increasing amounts of E2, proportional changes in E2 concentration were measured in the dialysate. Moreover, a rise in E2 concentration was detected after in vivo retrodialysis of testosterone only if the probe was located within the POM and, after a peripheral injection of E2, a sharp rise of E2 was detected regardless of the probe location. Together these results show that in vivo microdialysis is a valid method to assess endogenous fluctuations of brain E2 concentrations in behaving animals. Two independent experiments then identified a rise in E2 concentrations in POM during sexual interactions. This increase occurred within 10 min after the initiation of the sexual interaction and was specific to the POM as there was no increase in E2 concentrations in males that had their cannula outside of this area. Together these data confirm that rapid changes in AA measured ex vivo cannot be considered as a reliable proxy for E2 concentrations. The discrepancies could originate either from the different time resolution related to the two techniques or from differences in the microenvironment in which aromatase functions in vivo and during ex vivo assays. [less ▲]

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See detailRaman Chemical Imaging in Kidney Stone Analysis
Castiglione, Vincent ULiege; Sacre, Pierre-Yves ULiege; CAVALIER, Etienne ULiege et al

Poster (2017, November 02)

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of ... [more ▼]

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of two-dimensions maps of the constituents' distribution in samples. We aimed at determining the use of RCI in urinary stone analysis. Methods: Twelve calculi were analyzed by RCI using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Prior to the analysis, samples were sliced and milled in order to detect the nucleus of the stones and having a smooth surface. RCI was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy, the gold standard method for the determination of urolithiasis composition. Results: RCI succeeded in identifying all the chemical components contained in each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, whitlockite and ammonium urate. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones, allowing the detection of very small structures like Randall's plaques. Moreover, RCI provided the distribution of components within the stones. The nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the center of one sample. Conclusion: RCI showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. In addition, RCI is nondestructive enabling the storage of samples. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, which makes it more suitable for research studies rather than routine analysis. [less ▲]

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See detailEssay of Siganus sutor aquaculture in Madagascar: for promising alternative activity for traditional fishermen
Ravelohasina, Helga; Rasolofonirina, R.; Mahafina, J. et al

Poster (2017, November)

Detailed reference viewed: 16 (1 ULiège)
See detailAnycast on the Move - A First Look at Mobile Anycast Performance
Wassermann, Sarah ULiege; Rula, John P.; Bustamante, Fabian

Poster (2017, November)

Service providers rely on replication to improve service performance and reliability, placing instances in multiple locations and redirecting clients to nearby copies. Anycast is a common mechanism used ... [more ▼]

Service providers rely on replication to improve service performance and reliability, placing instances in multiple locations and redirecting clients to nearby copies. Anycast is a common mechanism used for redirecting clients in a variety of domains from naming to CDNs and video streaming. IP anycast offers a method for making a service IP address available to a routing system from several locations at once, and clients' requests are directed based on BGP routing policies. For operators, IP anycast offers an economic, scalable, and simple approach to replicated services; BGP provides considerable robustness, adapting to changes in service and network availability. For clients, however, the mapping can be suboptimal, unstable, and seemingly chaotic, as routing policies have not only technical motivations, and routing changes can silently shift traffic from one site to another with a consequent loss of shared state and potential performance impact. Given its wide deployment and interesting tradeoffs, IP anycast has been the focus of much recent measurement work. All prior studies have, nevertheless, focused on wired networks despite the growing dominance of mobile Internet. Today, the number of mobile subscriptions is over 7.4 billion, and users spend over 2x times more hours browsing on their smartphones than on any other device, with the corresponding increase on cellular traffic. We present early results on the first study of anycast performance for mobile users. Our evaluation focuses on two distinct anycast services, K-and F-Root, each providing part the DNS Root zone. Both services are widely replicated with publicly available site locations and unicast IP addresses that allow us to evaluate the relative performance of anycast routing to its "optimal" (in terms of unicast) site location. We collected active measurements from geographically distributed clients on both cellular and WiFi networks from September 2016 until April 2017, using the Aqualab’s ALICE engine [1]. In each experiment clients launched ping and traceroute measurements towards the root servers’ anycast addresses, as well as to five chosen unicast addresses determined to be the closest to the client in terms of geographic distance, at an hourly rate. Clients also recorded their geographic location, anonymized to a 10 km² area. Our findings show that mobile clients are routed to suboptimal replicas in terms of geographical distance, more frequently while on a cellular connection than on WiFi, with a significant impact on perceived service performance. The phenomenon seems to be more pronounced for K-Root than for F-Root. A possible explanation for the long distances would be that our cellular clients are simply far away from all the available replicas. However, our investigations demonstrate that this is not necessarily the case. Finally, we start to explore the root causes for anycast anomalies in cellular networks. We reveal three classes of anomalies: distant client packet gateways, poor anycast routing within Tier-1 networks, and improper routing out of cellular networks. [1] http://aqualab.cs.northwestern.edu/projects/261-alice [less ▲]

Detailed reference viewed: 36 (5 ULiège)
See detailBIGMOMAL - Big Data Analytics for Mobile Malware Detection
Wassermann, Sarah ULiege; Casas, Pedro

Poster (2017, November)

Mobile malware is on the rise. Due to their popularity, smartphones represent an attractive target for cybercriminals, especially regarding unauthorized access to private user data; smartphones ... [more ▼]

Mobile malware is on the rise. Due to their popularity, smartphones represent an attractive target for cybercriminals, especially regarding unauthorized access to private user data; smartphones incorporate a lot of sensitive information about users, even more than a personal computer. Indeed, besides personal information such as documents, accounts, passwords, contacts, etc., smartphone sensors centralize other sensitive data such as user location, physical activities, etc. In this paper, we study the problem of malware detection in smartphones, using supervised machine learning models and big data analytics frameworks. Using a publicly available dataset for smartphone data analysis (the SherLock data collection, see http://bigdata.ise.bgu.ac.il/sherlock/), we train and benchmark different supervised machine learning models to detect malware apps activity.The Sherlock data collection is a crowdsourcing-based smartphone dataset in which hundreds of features from many different "sensors" or vantage points within the device are monitored, using a tailored smartphone agent. The collection is done during a long-term - 2 years (2015/16), field trial on 50 smartphones used as primary device for 50 different participants. The monitoring agent collects a wide variety of network, software and sensor data at a high sample rate (as low as 5 seconds); in addition, participant devices include a sandbox-like smartphone agent which runs controlled malware apps, perpetrating attacks on the user's device (such as contacts theft, spyware, phishing, etc.), while creating labels for the SherLock dataset. The complete labeled dataset contains more than 10 billion data records, with a total of about 4 TB of data. We additionally complement the labels for malicious apps which might have been installed by participants by analyzing the installed apps' hashes in Virus Total (https://www.virustotal.com), a well-known multi antivirus online scanning system. From the complete dataset, we keep two specific feature categories: all those features related to the network traffic generated by the apps, and all those features corresponding to the footprint of the app on the CPU and internal running processes (e.g., statistics on CPUs, memory usage, linux-level processes information, etc.). The rationale is that some malware activity would be more visible at the network traffic level, whereas some others would be better identified at the local processes level. Using this dataset, we train different machine learning models (e.g., decision trees, neural networks, SVMs, etc.) and verify their accuracy to automatically spot out malicious apps running on the users’ devices. We also apply feature selection strategies to improve results and reduce computational times. Given the size of the dataset, we rely on big data platforms (such as Spark) to perform the analysis, complementing the machine learning based analysis with scikit-learn like pipelines. We evaluate three different concepts, including (i) overall model performance (using multi-fold cross validation on the complete dataset), (ii) generalization of the learned models across different users (train in N-1 users, and test in the remaining user), and (iii) detection accuracy drift along time (train during first month/week, test the resulting model in the subsequent months/weeks). Initial results are very promising, especially regarding overall model performance for decision tree based models. [less ▲]

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See detailCoalescence in Highly Viscous System
Leleu, David ULiege; Pfennig, Andreas ULiege; Bruns, Nicole

Poster (2017, November)

study of the coalescence in liquid-liquid dispersion by comparing batch settling cell experiment with a numerical tool, based on ReDrop concept.

Detailed reference viewed: 7 (1 ULiège)
Peer Reviewed
See detailENTOFÔR project -From waste to resource
Lecrenier, Marie-Caroline ULiege; Fumière, Olivier; Berntssen, M.H.G. et al

Poster (2017, November)

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See detailCarbon and nutrients sources and transformation in the River Sabaki, Kenya
Tamooh, FT; Shawlet, C; Borges, Alberto ULiege et al

Poster (2017, October 30)

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See detailUpscaling winter wheat above-ground biomass measurements using multispectral imagery and 3D data from unmanned aerial vehicle
Michez, Adrien ULiege; Bauwens, Sébastien ULiege; Heinesch, Bernard ULiege et al

Poster (2017, October 20)

Field measurements in the ICOS program are spatially limited whereas the monitored gas fluxes may have a large footprint. Aerial remote sensing has the advantage to monitor large areas. The main goal of ... [more ▼]

Field measurements in the ICOS program are spatially limited whereas the monitored gas fluxes may have a large footprint. Aerial remote sensing has the advantage to monitor large areas. The main goal of our research was to test the potential of unmanned aerial vehicles (UAV) to upscale parameters monitored through the ICOS program. In this study, we specifically focus on above-ground biomass (AGB) monitoring in a winter wheat crop. We used a octocopter drone (X frame type) to acquire a time series over the crop growing season (8 flights from the 14th of February 2017 to the 7th of July 2017) of multispectral imagery covering the ICOS candidate station of Lonzée (Wallonia, Belgium) and the surrounding field crop areas (ca. 0.25 km² per flight). The multispectral camera provides spectral information on the green (550 +/- 50 nm), red (660 +/- 50 nm), near infrared (735 +/- 50 nm) and red-edge (790 +/- 10 nm) wavelengths bands. The UAV also brought an off-the-shelf high resolution (20 Mpx) RGB camera to derive accurate 3D data. We performed a photogrammetric 3D reconstruction of the acquired imagery for every flight survey. The images provided by the RGB sensor (Sony RX100) were used to produce a high spatial resolution Digital Surface Model (0.05 m) and the images acquired by the multispectral sensor were used to derive reflectance maps (0.1 m) in the four wavelengths bands. The four reflectance layers were combined to produce two straight-forward vegetation indices (Normalized Difference Vegetation Index and Green NDVI). The photogrammetric DSM’s were combined to a LiDAR Digital Terrain Model (public database, survey in winter 2013) to produce Crop Height Models (CHM) of the study area. We used multiple linear regressions modelling in order to predict the AGB of the field crop monitored by the ICOS station of Lonzée with UAV imagery. AGB=a+b*GNDVI+c*NDVI+d *CHM The field crop data were provided by the ICOS program and by field research conducted in experimental field crops close to the flux tower. The field sampling consisted in destructive samples of the crop which were weighted after drying. For each field sample, an associated area was computed based on the outdistance sowing and the number of sampled crops in order to compute an AGB per area unit (t / Ha). Each AGB field estimation was associated to the closest flight date to build a multi date model presenting good performances (r² = 0.85, RMSE = 2.3 t/Ha). We used the same modelling approach to adjust a single date model to derive a predicted AGB map for the 7th of July. The performance of the single date model is lower but still highlights the biomass variation within the crop (r² = 0.71, RMSE = 1.9 t/Ha). The predicted AGB map displays a high spatial heterogeneity with some spatial patterns. Locally low AGB values are found along two old pedestrian whereas higher AGB values can be associated to areas which were sprayed twice (in-between two tractor tracks). Our results highlight the potential of UAV multispectral imagery to monitor the AGB variation within the footprint of the flux tower and highlight the need for repeated field sampling with a precise geolocation to improve the matching between the flight and the field surveys. [less ▲]

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See detailPhosphorous from Sludge: Extraction Expertise at ULiège
Pfennig, Andreas ULiege; Shariff, Zaheer Ahmed ULiege

Poster (2017, October 18)

In the PhosForYou project, the PASCH process, formerly designed to recover phosphorous from sewage-sludge incineration ash, is extended to start from dried sewage sludge directly. One of the major steps ... [more ▼]

In the PhosForYou project, the PASCH process, formerly designed to recover phosphorous from sewage-sludge incineration ash, is extended to start from dried sewage sludge directly. One of the major steps in this process is the reactive extraction of heavy metals from a leach liquor, followed by fractional precipitation. The process is realized on pilot-plant scale and tested in bypass at waste-water treatment plants. The process param-eters will be optimized to maximize process efficiency depending on the local situation with respect to impurities and water content. The product is a phosphate, which can directly be used in fertilizer formulations. [less ▲]

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Peer Reviewed
See detailQuality assessment of marketed eggs in Hanoi (Vietnam)
Moula, Nassim ULiege; Luc, Do Duc; Bo, H. X. et al

Poster (2017, October 13)

Poultry farming is the first sector of livestock that has been industrialized. The poultry sector plays an important role in the GDP of Vietnam. It is the second largest livestock after the pig livestock ... [more ▼]

Poultry farming is the first sector of livestock that has been industrialized. The poultry sector plays an important role in the GDP of Vietnam. It is the second largest livestock after the pig livestock. The production and consumption of eggs in Vietnam is estimated as 5.64 billion and 102.6 eggs per person per year respectively. The aim of this study was to assess the quality of eggs in relation to the chicken breed and different marketing channels in Hanoi, Vietnam. In total, 431 chicken eggs were purchased from four different marketing channels in Hanoi, including households (89 eggs), supermarkets (152 eggs), public markets (130 eggs) and small grocery stores (50 eggs). Out of the 431 above-mentioned eggs, 119 eggs came from the commercial chicken breeds and 312 eggs from traditional local breeds. The quality of eggs for a consumer is represented by its cleanliness, nutritional quality, freshness, and price. A series of measurements is carried out just after their purchase. According to the breed and the marketing channel, highly significant differences (P <0.05) were found in the freshness of the eggs (Haugh units), egg prices, eggs weight, egg shell, the white and the yolk of the egg. Though the chicken breed and marketing channels do not significantly affect (P> 0.05) the freshness of the eggs, however, they have a significant effect (P <0.05) on the size of the eggs marketed in Hanoi. The results of this study show an important diversity in marketing channels and marketed eggs in Hanoi. [less ▲]

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See detailInventory of Beekeeping in the Algerian north (Tizi-ouzou and Bejaia)
Yahi, Krimou; Touazi, Leghel; Kaidi, Rachid et al

Poster (2017, October 13)

The objective of this study is to make an inventory of the honey production in the counties of Bejaia and Tizi-Ouzou in northeastern Algeria. To achieve this, a survey was carried out on 31 beekeepers (14 ... [more ▼]

The objective of this study is to make an inventory of the honey production in the counties of Bejaia and Tizi-Ouzou in northeastern Algeria. To achieve this, a survey was carried out on 31 beekeepers (14 in Bejaia and 17 in Tizi-Ouzou). The survey shows a social, cultural and religious role for 61.29% of the beekeepers. The beekeeping is primarily held by men (93.55%). It is the basic source of income for 64.52% of the respondents. The average age of the beekeepers is 42.90years (Min-Max: 28-67 years, Median: 40.5 years). The main products of the hive are honey (100%), swarms (16.13%), royal jelly (9.68%) and propolis (9.68%). The average honey production per hive is 7.70kg (Min-Max: 0.4-15kg hives, Median: 10kg). The average number of hives per beekeeper is 42.20 hives (Min-Max: 3-300 hives, Median: 17.50 hives). The average selling price of one kg of honey is 4000.00 DA / kg (Min-Max: 2000-5000DA; Median: 4000DA). The phenotype of the bee reported by 74.19% of the surveys is of small size with a long body and dark pigmentation corresponding to the breed “Apis mellifera intermissa”. Two apiculturists described another phenotype corresponding to the “Apis mellifera major”. The factors behind the motivations for beekeeping are consumption of honey (100%), income generation (90.32%), hobby (58.06%) and conservation of biodiversity (22.58%). The multiple constraints associated with several diseases, notably Varroase (mentioned by 80.65% of beekeepers), cause difficulties for the breeders. Thus they cannot profit maximum from beekeeping. Other constraints which were reported are; forest fires (35.48%), wasps (32.26%), absence of beekeeping professionals or technicians (29.03%), harsh and cold winters with snow (19.35%), high density of hives in the region (16.13%) and uncontrolled spreading of pesticides and crop protection products at farms (12.90%). The economic situation of the Algerian beekeepers can be optimized by improving the production potential of the local bees. [less ▲]

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See detailComparison of the effects of Opuntia ficus-indica powder on growth performance and serum parameters of the Broiler Chicken in Algeria
Moula, Nassim ULiege; Humbel, Maïlis ULiege; Leterrier, Mélanie ULiege et al

Poster (2017, October 13)

In Algeria, the ingredients used in the broiler feed are exclusively imported from abroad, which affects negatively the production cost of the chicken meat and its sale price in the market at national ... [more ▼]

In Algeria, the ingredients used in the broiler feed are exclusively imported from abroad, which affects negatively the production cost of the chicken meat and its sale price in the market at national level. Because of the wide diversity in soil and climate, Algeria has a substantial number of plants which can be used in animal feed. This work is part of the valuation of the feed potential of the barbaric fig tree, widely present in the Algerian rural landscape, for the broiler chicken. The Opuntia ficus-indica is known for its edible fruits and for use of its fleshy leaves or “racquets” as fodder especially during periods of drought. It is also used to control the water and wind erosion as well as for the protection and improvement of soil fertility in arid and semi-arid regions. The aim of this study was to measure the effects of Opuntia ficus-indica powder on growth performance, serum composition and carcass yield of broiler chickens. The experiments were performed in a private poultry farm in the Chemini region (Wilaya de Bejaia). In this study, 120 Ross-308 day-old male chicks were monitored. They were divided in 3 groups (Group 1, 2 and 3) according to the specific diet (4 x 10 chicks / group). The group 1 was offered commercial feed. The group 2 and group 3 were offered the same commercial feed as group 1 but 5% and 10% of the commercial feed was replaced by the Opuntia ficus-indica powder respectively. The Opuntia ficus-indica powder did not show any negative effect (p>0.05) on the final body-weight, average daily gain and carcass yield. However, it decreased (p<0.05) the biochemical parameters -blood concentration- (plasma glucose, uremia, cholesterol and triglycerides). In conclusion, the Opuntia ficus-indica powder has the potential to be used in poultry feed to reduce the cost of broiler feed in Algeria [less ▲]

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See detailClinical and haematological alterations in foot and mouth disease virus naturally-infected domestic water buffaloes in Vietnam
Dam Van Phai, ULiege; BUI TRAN ANH, Dao, ; Desmecht, Daniel ULiege

Poster (2017, October 13)

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at ... [more ▼]

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at observing clinical indicators, physiological parameters, haematological and blood chemistry values of 30 buffaloes naturally infected with FMD virus (FMDV). The study was undertaken from January 2015 to February 2016 in Hanoi, Vietnam. Results show that buffaloes infected with FMDV displayed significantly higher fever, increased respiratory rate and increased heart frequency compared to healthy animals. Moreover, FMDV-infected animals always showed high fever and vesicular stomatitis, often showed edging nail congestive inflammation and, sometimes only, blisters on the nipple. Haematological indicators showed that FMDV-infected buffaloes were dehydrated (increased total protein). Besides, infected animals also underwent anaemia as mean corpuscular haemoglobin and mean corpuscular haemoglobin concentrations were plummeted. Total white blood cell count plummeted too, especially the number and proportion of neutrophils. Meanwhile, the number and proportion of lymphocytes sharply increased compared to healthy animals. Infected buffaloes also displayed signs of liver and heart damages as judged by an increase in concentration of AST and ALT, while there was not renal damage since creatinine concentration remained stable [less ▲]

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See detailElectrode materials for Li/Na-ion batteries: Improving electrochemical performance through carbon addition during synthesis
Mahmoud, Abdelfattah ULiege; Karegeya, Claude; Eshraghi, Nicolas ULiege et al

Poster (2017, October 12)

Lithium-ion batteries have been widely applied as a power source for portable and stationary energy storage systems. Na-ion batteries are considered to be an alternative to Li-ion batteries owing to the ... [more ▼]

Lithium-ion batteries have been widely applied as a power source for portable and stationary energy storage systems. Na-ion batteries are considered to be an alternative to Li-ion batteries owing to the natural abundance of sodium. New electrode materials are required to increase the energy density of Li/Na-ion batteries. In this study, we show that the addition of the carbon sources during the synthesis leads to control the particles size and morphology and improve their conductivity properties that enhance the electrochemical performance [1-5]. In order to study the effect of the carbon on the structural, morphological and electrochemical properties of the prepared materials by a spray-drying [1-3] or hydrothermal methods [4, 5]. The crystal and local structure were analyzed by XRD and Mössbauer spectroscopy. The morphological properties were characterized by SEM and TEM. The carbon content was determined by TG/TDA and carbon analyzer. The electrochemical properties were studied by impedance spectroscopy and galvanostatic cycling in lithium cells. Finally, the reaction mechanism during cycling was investigated using operando XRD technique. 1- A. Mahmoud, S. Caes, M. Brisbois, R.P. Hermann, L. Berardo, A. Schrijnemakers, C. Malherbe, G. Eppe, R. Cloots, B. Vertruyen, F. Boschini, Spray-drying as a tool to disperse conductive carbon inside Na2FePO4F particles by addition of carbon black or carbon nanotubes to the precursor solution, J. Solid State Electrochem. (2017) 1–10. 2- N. Eshraghi, S. Caes, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Sodium vanadium (III) fluorophosphate/carbon nanotubes composite (NVPF/CNT) prepared by spray-drying: good electrochemical performance thanks to well-dispersed CNT network within NVPF particles, Electrochim. Acta, 228 (2017) 319–324. 3- M. Brisbois, S. Caes, M-T. Sougrati, B. Vertruyen, A. Schrijnemakers, R. Cloots, N. Eshraghi, R-P. Hermann, A. Mahmoud, F. Boschini, Na2FePO4 F/multi-walled carbon nanotubes for lithium-ion batteries: Operando Mössbauer study of spray-dried composites, Solar Energy Materials & Solar Cells 148 (2016) 67-72. 4- C. Karegeya, A. Mahmoud, B. Vertruyen, F. Hatert, R.P. Hermann, R. Cloots, F. Boschini, One-step hydrothermal synthesis and electrochemical performance of sodium-manganese-iron phosphate as cathode material for Li-ion batteries, J. Solid State Chem.253 (2017) 389–397. 5- C. Karegeya, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Hydrothermal synthesis in presence of carbon black: Particle-size reduction of iron hydroxyl phosphate hydrate for Li-ion battery, Electrochimica Acta. Electrochim. Acta 250 (2017) 49–58. [less ▲]

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See detailIntramolecular continuous-flow strategy towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULiege; Winter, Marc; Vizza, Alessandra et al

Poster (2017, October 12)

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See detailRelationship between brain activity and ocular movements during wakefulness and drowsiness
François, Clémentine; Wertz, Jérôme; Verly, Jacques ULiege

Poster (2017, October 11)

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See detailPhysico-chemical Approach for Characterising Probiotics: example of High Concentrated Multistrain-Based Formulation
Razafindralambo, Hary ULiege; Delvigne, Frank ULiege; Blecker, Christophe ULiege

Poster (2017, October 09)

Probiotics are receiving today unprecedented growing interests in Europe, Asia, and in the rest of the world. Their applications are not only limited to food and health sectors, but cover also agriculture ... [more ▼]

Probiotics are receiving today unprecedented growing interests in Europe, Asia, and in the rest of the world. Their applications are not only limited to food and health sectors, but cover also agriculture and aquaculture areas. The quality of probiotic products depends on many factors such as, the properties of each individual strain and its proportion in mixed products, the viable probiotic dose, and other selective ingredients like prebiotics and protectant agents incorporated into the formulation. Several strategies are employed for ensuring high product qualities, which can be controlled by different methods and techniques. To date, the physico-chemical approach for characterizing and controlling probiotic qualities and performances appears very attractive, but less exploited. It particularly consists in characterizing probiotic products in terms of thermal, surface and colloidal properties, which could be correlated to probiotic viability and functionalities. In this communication, we report the efficiency of such an approach when applied to a high concentrated multistrain-based formulation which has been shown a metabolic variability impacting on the inflammatory response, depending on the production site. [less ▲]

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See detailThe LNQ25 and ELN PVT Metrics Exhibit a Good Sensitivity to Sleep Deprivation and are Independent from the Subject
Latour, Philippe ULiege; Van Droogenbroeck, Marc ULiege

Poster (2017, October 09)

Introduction Performance of people undergoing critical tasks (like driving) may be impaired completely by the lowering of their vigilance level, due to sleep deprivation for instance. This reduction of ... [more ▼]

Introduction Performance of people undergoing critical tasks (like driving) may be impaired completely by the lowering of their vigilance level, due to sleep deprivation for instance. This reduction of performance may be measured by metrics computed from the reaction times (RT) recorded during a 10min Psychomotor Vigilance Test (PVT). Here, we analyze and compare the sensitivity to sleep deprivation and the subject dependent variability of the PVT metrics performance, with a special emphasis on the time interval sizes. Materials and Methods Individuals (22 volunteers; 11 males, 11 females, mean 22.2y., range 19-34 years) follow an uninterrupted 28h sleep deprivation standard PVT protocol during which they achieved two groups of three PVT sessions (in different conditions). The first PVT of each group is in Non-SDP condition (9h30 and 10h30 Day 1) and the second and third PVT of each group are in SDP condition (2h30, 3h30, 10h30 and 11h30 day 2). The subjects fill a sleep journal during the week before the experiment. We checked that they had a normal sleep-wake cycle with no sleep deprivation, jet-lag or shift work and no medication. During the PVT of the first group, the subjects were wearing the glasses of the Phasya’s Drowsimeter. We compute the usual PVT metrics; meanRT, meanRS (Reaction Speed) and LN500 (500ms lapses number). We also compute LNQ25 (adaptive lapses number computed with a subject dependent threshold) and ELN (Expected Lapse Number, computed from a subject-dependent estimation of the lapse probability). Results We use the “Effect Size” (ES, ratio of the mean by the standard deviation of the difference of metrics in the SDP and Non-SDP conditions) to assess the sensitivity to sleep deprivation. For the 10min (resp. 1min, 3min) interval, the ES of LNQ25 and ELN are respectively 1.38 (resp. 0.95, 1.22) and 1.35 (resp. 0.85, 1.14), the ES of meanRS, meanRT and LN500 are 1.23 (resp. 0.91, 1.09), 0.81 (resp. 0.54, 0.68) and 0.85 (resp. 0.63, 0.77). We classify the intervals on which metrics are computed as SDP or non-SDP. We use a fixed threshold for the metrics, independent of the subject. In the ROC curves, the TPR (for a FPR of 10%) assesses the quality of the classification, and then also the subject independence. For the 10min (resp. 1min, 3min) interval, the TPR of LNQ25 and ELN are respectively 0.86 (resp. 0.56, 0.75) and 0.83 (resp. 0.58, 0.75), the TPR of meanRS, meanRT and LN500 are 0.42 (resp. 0.38, 0.41), 0.40 (resp. 0.39, 0.40) and 0.42 (resp. 0.24, 0.30). Conclusions We demonstrate that LNQ25 and ELN enable a quite good classification of the SDP condition for time intervals greater than or equal to 3min, independently of the subject. On the other hand, these metrics provide also a good sensitivity to sleep deprivation. They outperform the usual metrics for both criteria. For time intervals below 3min, the performances degrade first progressively and then much more quickly below 1min. [less ▲]

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See detailRuminal function influenced by diet parameters in dairy herds with milk fat drop syndrome in Belgium
Knapp, Emilie ULiege; Sartelet, Arnaud ULiege; Malniece, Aija et al

Poster (2017, October 06)

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See detailAbdominal steatonecrosis in a twelve-year old Highland bull.
Casalta, Hélène ULiege; Bayrou, Calixte ULiege; Denis, Marine ULiege et al

Poster (2017, October 05)

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See detailAtypical polypoid leiomyosarcoma in an ouessant ewe.
Bayrou, Calixte ULiege; Casalta, Hélène ULiege; Cassart, Dominique ULiege et al

Poster (2017, October 05)

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See detailQuantitative profiling of endogenous polar metabolites from low volumes of blood samples
Kok, Miranda ULiege; Fillet, Marianne ULiege

Poster (2017, October 05)

The etiology of many diseases is not yet completely understood. The involved biological processes might be resolved using a metabolomics approach, because metabolomics provides unique challenging ... [more ▼]

The etiology of many diseases is not yet completely understood. The involved biological processes might be resolved using a metabolomics approach, because metabolomics provides unique challenging opportunities to correlate the metabolome with a physiological or pathophysiological status and provides a vision on the relationships between genes, gene expression, environment and lifestyle. Here, we present the development of two ultra-high performance liquid chromatography (UHPLC) methods coupled to mass spectrometry (MS) for the separation and quantitation of polar metabolites in blood samples. A reversed-phase UHPLC-MS/MS method has been developed to quantify anionic energetic metabolites, whereas hydrophilic interaction chromatography (HILIC)-MS/MS has been used to determine amino acids. Two sample pretreatment procedures have been developed for an optimal recovery of the respective metabolites from whole blood samples. One method involved the precipitation of proteins with acetonitrile and acids. In addition, volumetric absorptive microsampling has been used for the sample preparation. Small and accurate quantities of biological fluids (10 or 20 µL) can be collected with this sampling technique, which is of great interest for volume-limited samples or serial collection of samples. The developed methods have been validated and will be applied to determine differences in metabolite concentrations between plasma samples from patients and controls. This can lead to a breakthrough in the understanding of diseases and can open new therapeutic perspectives. [less ▲]

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See detailOntogenetic and phylogenetic simplification during white stripe evolution in anemonefish
Salis, Pauline; Roux, Natacha; Soulat, Olivier et al

Poster (2017, October 04)

Coral reef fishes provide classical examples of complex colour patterns exhibiting a huge diversity. Most of these species display spots, stripes, repeated lines, eyespots, grids, etc. This diversity in ... [more ▼]

Coral reef fishes provide classical examples of complex colour patterns exhibiting a huge diversity. Most of these species display spots, stripes, repeated lines, eyespots, grids, etc. This diversity in colour patterns might serve for species recognition, camouflage, mimicry and/or warning. To date, work have mainly been focused on the link between colour patterns, ecology and behavior, that is the ultimate role of these patterns. However, the underlying development and cellular mechanisms controlling these patterns and their evolution, that is their proximal mechanisms, are still largely unknown. To address this question, we are using a well-known coral-reef fish models, anemonefishes (Amphiprion and the monotypic Premnas). This tribe (Amphiprionini) within the Pomacentridae is composed of 30 species that display a relatively simple colour pattern made of 0-3 white stripes that are well visible on a yellow to red, brown or even black body background. This simple colour pattern offers a unique opportunity to better delineate the pattern and processes allowing the diversification of such diversity. Here, we focus on the vertical white stripes present in most species of Amphiprion. We first map their striped patterns on the anemonefish evolutionary tree and reconstruct the ancestral state. Our results provide evidences that the diversification in colour pattern in anemonefish results from successive losses of stripes during evolution. Then, by an ontogenetic study, we show that larvae stripes always appear from rostral to caudal. Interestingly, larvae of some species such as A. frenatus have surplus stripes (with a maximum of three stripes) which disappear caudo-rostrally during the juvenile phase to acquire their adult color pattern. The reduction of stripes number over ontogeny totally matches the sequences of stripe losses across evolution. This demonstrates that the diversification in colour pattern among anemonefish lineages results from changes in developmental processes. Finally, assuming that the number of stripes may be related to the species ecology, we further determined the links between the number of stripes and ecomorphological traits. Together, this innovative study allows to understand how developmental processes are shaping the diversification of color pattern of anemonefishes and how it may be related with ecomorphological traits evolution. [less ▲]

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See detailValidité et utilité d'un test d'orientation dans le secteur de la santé en Fédération Wallonie Bruxelles
Detroz, Pascal ULiege; Monseur, Christian ULiege; Romainville, Marc et al

Poster (2017, October 04)

La Fédération Wallonie Bruxelles de Belgique n’avait pas, jusqu’en 2016, de Filtres à l’entrée des études de Médecine. Elle avait par contre un test diagnostique, formatif, obligatoire, mais non ... [more ▼]

La Fédération Wallonie Bruxelles de Belgique n’avait pas, jusqu’en 2016, de Filtres à l’entrée des études de Médecine. Elle avait par contre un test diagnostique, formatif, obligatoire, mais non contraignant (il n'avait pas d'effet sur l'avenir des étudiants). Ce test se présentait sous la forme d’un questionnaire à choix multiple (QCM) évaluant plusieurs matières : la chimie, la physique, la biologie, les mathématiques, le français et l’anglais. Il se déroulait en même temps sur le site des cinq universités concernées par les études dans le domaine de la santé de la FWB. Les questions du test sont identiques dans ces différentes universités. Il a été organisé entre 2013 et 2016 , deux fois par année en juillet et septembre. L’enjeu était de permettre aux étudiants de mesurer leur compétence à l’entrée des études pour se rassurer (pour les plus fort d’entre eux), pour s’améliorer à travers des activités de remédiation, ou éventuellement pour renoncer (pour les pl us faible d’entre eux). La présente étude a pour objet d’évaluer l’efficacité de ce test diagnostic. Concrètement, les objectifs de l'étude décrite étaient de/d’ : 1. Analyser la qualité psychométrique du test d'orientation. 2. Établir le lien entre les données biographiques de l’étudiant et les résultats au test d'orientation. 4. Établir le lien entre les résultats au test d'orientation et l’inscription réelle en faculté de médecine.(l'effet de dissuasion du test) 3. Établir le lien entre le test d'orientation et la réussite globale et par matière à la fin de la première année. Nos conclusions générales sont les suivantes : Les tests d’orientation présentent une bonne qualité psychométrique. C’est toutefois plus vrai pour les disciplines scientifiques que pour l’anglais et le français. Le test vise bien les aptitudes spécifiques et les compétences prérequises pour entreprendre des études supérieures dans le secteur de la santé. Les candidats proviennent d’un milieu socio-économique élevé et présentent un parcours scolaire au dessus de la moyenne. Ces variables expliquent, chez les étudiants diplômés du secondaire en Belgique, plus de 30% de la variance du score de sciences au test d'orientation (score factorisé à partir des scores de maths, bio, chimie et physique) et plus de 10% du score de langue (score factorisé à partir des scores de français et d’anglais) à ce même test. Ces mêmes variables de présage expliquent aussi en partie le résultat en fin de premier bachelier (22% de variance expliquée pour les étudiants diplômés en Belgique, 34% pour les étudiants diplômés en France). Les résultats aux tests d’orientation, quant à eux, expliquent ensemble 29% de la variance aux résultats de bac1 pour les étudiants diplômés en Belgique et 17% pour ceux diplômés en France. Le test de sciences s’avère beaucoup plus prédictif que le test de langues Les test dissuade peu les étudiants les plus faibles de s’inscrire. Ces résultats seront présentés lors de la communication [less ▲]

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See detailNew low electron flux facility in the 0 to 3.5 MeV range for the study of induced signal in JUICE instruments: UVS and MAJIS measurements
Carapelle, Alain ULiege; Grodent, Denis ULiege; Langevin, Yves et al

Poster (2017, October)

We designed and built a new test facility to investigate signal induced by electrons in the 0-3.5 MeV in the JUICE UVS and MAJIS instruments. The facility uses radioisotopes sources to produce low flux of ... [more ▼]

We designed and built a new test facility to investigate signal induced by electrons in the 0-3.5 MeV in the JUICE UVS and MAJIS instruments. The facility uses radioisotopes sources to produce low flux of electrons (< 6000 electrons/cm².s). We present the facility, its capabilities and the results of measurements on UVS and MAJIS. [less ▲]

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See detailPrevalence and characteristics of group B streptococcus colonization in HIV-infected pregnant women in Belgium
DAUBY, Nicolas; ADLER, Catherine; Y MIENDJE DEYI, Véronique et al

Poster (2017, October)

Objectives: High incidence of GBS sepsis has been reported in HIV-exposed but uninfected (HEU) infants in both developed and developing countries, particularly late-onset diseases. We aimed determining ... [more ▼]

Objectives: High incidence of GBS sepsis has been reported in HIV-exposed but uninfected (HEU) infants in both developed and developing countries, particularly late-onset diseases. We aimed determining the prevalence, the characteristics and the risk factors of GBS carriage in HIV-infected and HIV uninfected pregnant women (PW). Methods : Between 1/01/2011 and 31/12/2013, HIV-infected (n=132) and uninfected (n=123) PW had recto-vaginal swabs for GBS detection performed at 35-37 weeks of gestation and at deliver Demographic, obstetrical and medical data related to HIV-infection were prospectively collected. Serotyping of GBS strains was performed on a limited number of randomly selected samples (26 from HIV-infected and 13 from uninfected PW). Results : The overall prevalence of GBS carriage was not statistically different between HIV-infected and uninfected PW (31% vs 24,4% respectively). Age, nadir CD4 cell count, CD4 cell count at delivery and detectable viral load at delivery were not associated with GBS carriage rate in HIV-infected PW. A distinct pattern of GBS serotype was found in HIV-infected PW who were predomina colonized by serotype III (12/26) while HIV-uninfected PW were mostly colonized by serotype Ia (8/13) (p<0,05). Conclusions: As previously reported in other countries, HIV-infected PW do not have significantly higher rate of GBS colonization. However, our results suggest that HIV-infected PW are more lik be colonized with serotype III strains, that is the main serotype associated with late-onset neonatal sepsis. Ongoing research aims at characterizing the clonal features of the isolated strains [less ▲]

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See detailLifetime of 5 non-commercial boluses evaluated in dairy cows by X Rays and plasmatic trace elements concentrations
Guyot, Hugues ULiege; Cheleux, Gaël ULiege; Wajda-Dubos, Jean-Pierre et al

Poster (2017, October)

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See detailPrevalence and capsular-polysaccharide type distribution of colonizing group B streptococci (GBS) isolated from recto-vaginal samples in pregnant women in Hanoï, Vietnam
MEEX, Cécile ULiege; DEVEY, Anaïs ULiege; PHAM HONG, Nhung et al

Poster (2017, October)

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of ... [more ▼]

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of GBS colonization among pregnant women in Hanoï and to characterize the capsular-polysaccharide (CPS) type of the isolated strains. Methods: For a 2-months period in 2015, 888 recto-vaginal swabs were collected in Bach-Mai-Hospital from pregnant women at 35-37 weeks’ gestation and were cultured for detection of GBS. Strains were stored and transferred to the Belgian NRC for further characterization. CPS-typing was performed by both latex agglutination and PCR (Poyart, 2007; Kong, 2008). Results: Among the 888 swabs, 111 were positive for GBS, that is a prevalence of colonization of 12.5%. A total of 90 strains were available for typing: 91,11% could be serotyped by latex agglutination and all the strains, including the 8 phenotypically non-typable strains, were successfully genotyped. CPS type V was the most prevalent (36.7%) followed by CPS types Ib (25.6%), III (21.1%), VI and VII (8.9% and 4.4%). CPS type II was found twice and serotype Ia was found once. CPS types IV, VIII and IX weren’t present in this population. Conclusion: With predominance of types V, Ib and III, this distribution of CPS-types of GBS colonizing pregnant women in Hanoï, Vietnam, differs from distributions described in Europe and in o Asian countries. This study provides useful information for the development of a universal vaccine that could contribute to improve the prevention of neonatal GBS infections. [less ▲]

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See detailEvolution of rate and genotypes of resistance to macrolide/lincosamide among invasive Group B Streptococcus (GBS): Development of a multiplex PCR tool for simultaneous detection of ErmB, ErmTr, MefA and LsaC resistance genes.
SACHELI, Rosalie ULiege; DESCY, Julie ULiege; MEEX, Cécile ULiege et al

Poster (2017, October)

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance ... [more ▼]

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance, ermB, ermTR, mefA and one gene for clindamycin-resistance lsaC. AdhP gene amplification was used as control for GBS identification. All(219) GBS isolates from invasive infections in newborns and adults received by the Belgian National Reference Center for GBS in 2015, and control strains were tested for erythromycin/clindamycin susceptibility (disk-diffusion/broth- microdilution) and for detection of resistance genes. Results: PCR products demonstrated the expected respective sizes. The method has been validated successfully according to ISO15189 analytical requirements. Of the 219 isolates, 67(30,67%) w resistant to erythromycin and/or clindamycin: 44/67(65,78%) showed a constitutive-MLS phenotype and 10/67(14,9%) the inducible-MLS phenotype. Among the constitutive-MLS strains, 73% harboured ErmB gene, 13% ErmTR, 7% ErmB+mefA and 7% ermB together with LsaC gene. The inducible-MLS strains harboured mostly ErmTr gene (89%) and the others the ErmB gene. Among the 10/67(14,9%) GBS strains with an M-phenotype (isolated resistance to erythromycin), the MefA gene was exclusively detected. Among the 3(4,48%) strains showing an isolated resistance to clindamycin (L-phenotype), the LsaC gene was detected. Conclusion: The developed multiplex PCR is able to detect simultaneously four genes involved in MLS resistance in GBS. In 2015, 30,6% of the invasive GBS strains isolated in Belgium were resist to macrolides and/or lincosamides. The emergence of the L-phenotype in GBS described since 2010, justifies the relevance to also detect LsaC gene together with ErmB, ErmTr and MefA. [less ▲]

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See detailUpdate of the characteristics of Group B Streptococci (GBS) colonizing pregnant women in Belgium: capsular-type distribution, pili characterization, antimicrobial susceptibility profile and Multiple Locus Sequence Types.
SACHELI, Rosalie ULiege; MEEX, Cécile ULiege; DESCY, Julie ULiege et al

Poster (2017, October)

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national ... [more ▼]

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national survey among pregnant woman. For each strain, determination of capsular-polysaccharide type agglutination and PCR, of pili-type by PCR and of antimicrobial susceptibility by disk-diffusion, broth-microdilution and detection of resistant genes by PCR. For serotype III strains, determination sequence-type by Multiple-Locus Sequence-Typing (MLST). Results: Serotype III was the most prevalent (28.5%) followed by serotypes V, Ia, II, IV and Ib (20.4%, 19.9%, 17.8%, 7%, 5.4%). Serotypes VI, VII and IX were found each once. All strains remained susceptible to penicillin (MICs: 0.03-0.125 mg/L) and other beta-lactams tested; 28.7% were resistant to erythromycin and 26.7% to clindamycin. With regards to pili, all 387 strains harboured one the PI-2 variants alone or in combination and 70.3% contained PI-1. The 110 serotype III isolates were resolved into 18 STs. The most common were ST-17 (35.5%) followed by ST-19 (30%) and ST- ST-27, ST-23 (<=5%). Conclusion: Among GBS from colonized pregnant women in Belgium: capsular-type and pili distributions, and MLST profile among type III strains were quite similar to reported data from Europ and USA during the last decade. As showed in this study, penicillin remains the first line drug of choice. On the contrary, resistance rates against macrolides/lincosamide, has reached a plateau since a decade, but it is noteworthy to notify the emergence of strains with isolated resistance to clindamycine. [less ▲]

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See detailIntramolecular Continuous-Flow Strategy Towards Methylphenidate (Ritalin) Hydrochloride
Gerardy, Romaric ULiege; Winter, Marc; Vizza, Alessandra et al

Poster (2017, October)

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See detailStratospheric circulation in the WACCM chemistry-climate model: mean age of air against observations and CTM
Minganti, Daniele ULiege

Poster (2017, October)

The mean age of air is a classical diagnostic of the transit time from the troposphere to the various regions of the stratosphere, providing insights on the strength and structure of the Brewer-Dobson ... [more ▼]

The mean age of air is a classical diagnostic of the transit time from the troposphere to the various regions of the stratosphere, providing insights on the strength and structure of the Brewer-Dobson Circulation (BDC), the polar vortex and the irreversible mixing in the mid-latitudes. We apply this diagnostic to the WACCM chemistry-climate model for the 1985-2014 period. A comparison is presented between unconstrained simulations (FR-WACCM) with and without the representation of the QBO, simulations nudged to the MERRA-2 reanalysis (SD-WACCM), in-situ measurements and a chemistry-transport model (CTM). The results are different depending on the simulation, with SD-WACCM resulting closer to the observations w.r.t. FR-WACCM. In the FR-WACCM the QBO plays a role leading to older age of air. The time evolution over the considered period is slightly decreasing, while observations show a positive (not significant) trend. Given those results, further studies have to be carried out investigating the multi-decadal trends of mean age of air and involving actual tracers. [less ▲]

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See detailPsychological Predictors and Consequences of Hearing Health Behaviors in Older Adults
Marquet, Manon ULiege; Chasteen, Alison L; Plaks, Jason E et al

Poster (2017, September 22)

Introduction: Hearing loss is a common chronic condition affecting older adults in Western populations [1, 2]. Even if older adults could benefit from hearing aid use, they are reluctant to wear them [1 ... [more ▼]

Introduction: Hearing loss is a common chronic condition affecting older adults in Western populations [1, 2]. Even if older adults could benefit from hearing aid use, they are reluctant to wear them [1]. The purpose of the present study was twofold: a) to examine whether older adults’ implicit theories regarding the modifiability of abilities in general would predict their hearing health behaviors; and (b) to assess consequences of these behaviors on subjective aging. Methods: One hundred and sixty-three older adults (Mean age = 68.62, SD = 5.40) completed, among other questionnaires, questions assessing their implicit theories of ability (i.e., seeing general abilities as fixed vs. improvable with effort [3]) and their subjective age (i.e., felt age, do age, look age, and interest age [4]). They also reported their perceptions of the benefits of various hearing health behaviors for slowing the effects of aging (i.e., getting their hearing tested and using hearing aids) as well as their intentions to engage in those behaviors. Results: We found that the more older people considered that abilities are alterable, the more they perceived hearing health behaviors as being beneficial, which in turn increased their intentions to engage in these behaviors. Also, the greater their intentions, the younger subjective age they reported. Key conclusions: These relationships between implicit theories, hearing health behaviors, and subjective age are particularly important to take into account. Indeed, neglecting hearing problems and experiencing an older subjective age may have detrimental health-related outcomes over time [5, 6]. 1. Feder, K., Michaud, D., Ramage-Morin, P., McNamee, J., & Beauregard, Y. (2015). Prevalence of hearing loss among Canadians aged 20 to 79: Audiometric results from the 2012/2013 Canadian Health Measures Survey. Health Reports, 26(7), 18-25. 2. Lin, F. R., Thorpe, R., Gordon-Salant, S., & Ferrucci, L. (2011). Hearing loss prevalence and risk factors among older adults in the United States. The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 66(5), 582-590. doi: doi:10.1093/gerona/glr002 3. Dweck, C. S. (1999). Self-theories: Their role in motivation, personality, and development. Philadephia, PA: Psychology Press. 4. Kastenbaum, R., Derbin, V., Sabatini, P., & Artt, S. (1972). “The ages of me”: Toward personal and interpersonal definitions of functional aging. The International Journal of Aging and Human Development, 3(2), 197-211. 5. Bainbridge, K. E., & Wallhagen, M. I. (2014). Hearing loss in an aging American population: Extent, impact, and management. Annual Review of Public Health, 35, 139-152. doi: 10.1146/annurev-publhealth-032013-182510 6. Kotter-Grühn, D., Kornadt, A. E., & Stephan, Y. (2015). Looking beyond chronological age: Current knowledge and future directions in the study of subjective age. Gerontology, 62(1), 86-93. doi: 10.1159/000438671 [less ▲]

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See detailMyoferlin controls mitochondrial structure in pancreatic ductal adenocarcinoma, and affects tumor aggressiveness
Rademaker, Gilles ULiege; Hennequière, Vincent ULiege; Brohée, Laura ULiege et al

Poster (2017, September 22)

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer, and the third leading cause of cancer related death. Therapeutic options remain very limited and are still based on ... [more ▼]

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer, and the third leading cause of cancer related death. Therapeutic options remain very limited and are still based on classical chemotherapies. Cell fraction can survive to the chemotherapy and is responsible for tumor relapse. It appears that these cells rely on OXPHOS for survival. Myoferlin, a membrane protein involved in cell fusion was recently shown by our laboratory to be overexpressed in pancreatic cancer. In the present study, we discovered that myoferlin was more expressed in cell lines undergoing oxidative phosphorylation (OXPHOS) than in glycolytic cell lines. In the former cell lines, we showed that myoferlin silencing reduced OXPHOS activity and forced cells to switch to glycolysis. The decrease in OXPHOS activity is associated with mitochondrial network disorganization. Dynamin-related protein (DRP)-1 phosphorylation led us to suggest mitochondrial fission, reducing cell proliferation, ATP production and inducing autophagy and ROS accumulation. To confirm the clinical importance of myoferlin in PDAC, we showed that low myoferlin expression was significantly correlated to high overall survival. Myoferlin staining of PDAC sections was negatively correlated with several 18FDG PET indices indicating that glycolytic lesions had less myoferlin. As the mitochondrial function is demonstrated to enhance the cell resistance to the treatment, the metabolic switch forced by myoferlin silencing could open up a new perspective in the development of therapeutic strategies. [less ▲]

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See detailTowards the development of a dual mode fluorescence/SERS dopamine aptasensor
Dumont, Elodie ULiege; De Bleye, Charlotte ULiege; Cailletaud, Johan ULiege et al

Poster (2017, September 21)

This work was dedicated to the development of a dual mode fluorescence and surface-enhanced Raman scattering (SERS) dopamine aptasensor. The preparation of the aptasensor was first optimised. Thereafter ... [more ▼]

This work was dedicated to the development of a dual mode fluorescence and surface-enhanced Raman scattering (SERS) dopamine aptasensor. The preparation of the aptasensor was first optimised. Thereafter, calibration curves were realised by means of fluorescence quenching and SERS. Finally, the specificity of both methods was tested. [less ▲]

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See detailAgeism and dementia stigma among healthcare professionals
Schroyen, Sarah ULiege; Adam, Stéphane ULiege; Missotten, Pierre ULiege

Poster (2017, September 21)

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See detailEffect of solution treatment on the fracture mechanisms of AlSi10Mg parts produced by Selective Laser Melting
Delahaye, Jocelyn ULiege; Mertens, Anne ULiege; Dedry, Olivier ULiege et al

Poster (2017, September 21)

Al alloy AlSi10Mg processed by Selective Laser Melting (SLM) displays high hardness and tensile strength due to its very fine eutectic microstructure [1]. However, as-built material exhibits low ductility ... [more ▼]

Al alloy AlSi10Mg processed by Selective Laser Melting (SLM) displays high hardness and tensile strength due to its very fine eutectic microstructure [1]. However, as-built material exhibits low ductility which is detrimental for applications where a high resilience is needed. To overcome this issue, solution heat treatment can be applied to soften and ductilize the material [2]. The very fine eutectic microstructure then turns into a dispersion of coarse globular Si precipitates. The distribution of Si precipitates seems to play a role in the fracture mechanism in as-built as well as solutionized tempers, but this effect is not yet fully understood [1,3]. Moreover, the lack of quantitative data makes it difficult to link precisely the fracture mechanisms to specific microstructural features. This study aims at investigating the role of the Si phase in the rupture mechanism, for both as-built and solutionized samples. It will focus more particularly on the heterogeneity of the distribution of Si precipitates where cracks are more likely to occur. Al cells size, along with the volume fraction and distribution of Si precipitates have been quantified by SEM image analysis. Al lattice parameter and Si crystallite size have been determined by X-ray diffraction/Rietveld analysis. For the image analysis, two types of specific zones have been observed i.e. the core and boundary of melt pools and Al grains, at different locations for representativity. These quantitative data have been compared with typical features observed on the fractured surface of tensile specimen. 1. D.-K. Kim et al. J. Alloys Compd. 686 (2016) 281. 2. W. Li et al., Mat. Sci. & Eng. A. 663 (2016) 116, 3. N.T. Aboulkhair et al. Mat. Sci. & Eng. A. 667 (2016) 139. [less ▲]

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See detailBreed fast, die young - Demography of a Neotropical fossorial frog
Székely, Diana ULiege; Székely, Paul; Stănescu, Florina et al

Poster (2017, September 19)

Information regarding age- and size-related parameters is of paramount importance both to understanding the life-history of populations, and for designing effective conservation strategies; however, such ... [more ▼]

Information regarding age- and size-related parameters is of paramount importance both to understanding the life-history of populations, and for designing effective conservation strategies; however, such base-line data lacks for many species, especially for those inhabiting tropical habitats. Using skeletochronology, we provide the first detailed information regarding the demography of the Pacific horned frog, Ceratophrys stolzmanni, a vulnerable, fossorial amphibian, that lives in the Tumbesian dry-forests of Ecuador. The species shows an intense growth rate during the first activity season, both before and after metamorphosis. Sexual maturity is achieved by both sexes before the first year of life, after which growth rates abruptly decrease. Females are on average larger, but not older, than males, mean age being two years in both sexes. The rapid sexual maturation is associated with a short life-span, the observed maximum longevity being three years in males and four years in females. The short reproductive life-span, which offers few mating opportunities, might have important implications for the conservation of this species, that can be especially at risk in the case of prolonged droughts expected to affect its habitat. [less ▲]

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See detailChallenges in the determination of amyloid oligomeric species by two electrophoretic techniques
Napp, Aurore ULiege; Houbart, Virginie ULiege; Demelenne, Alice ULiege et al

Poster (2017, September 18)

Parkinson’s disease is a frequent degenerative disorder, and for the moment the diagnosis is mainly clinical. When the first symptoms appear, loss of more than 70% of the dopaminergic cells already ... [more ▼]

Parkinson’s disease is a frequent degenerative disorder, and for the moment the diagnosis is mainly clinical. When the first symptoms appear, loss of more than 70% of the dopaminergic cells already occurred. Knowing that, it is of high interest to have one (or more) reliable biomarker(s) at our disposal to diagnose Parkinson before the first symptoms appear. Alpha-synuclein (aSyn) is a protein physiologically expressed at high level by neuronal cells, under a monomeric form. This protein would play a critical role in the development of the disease because under certain conditions, aSyn is capable of self-assembly to form fibrils like those found in Lewy bodies. Other intermediate soluble forms like dimers and oligomers are also formed. As these forms seems to be the toxic species, they are the center of many attentions. The quantification of each form would be a great help, but for the moment only the total forms (of monomeric or oligomeric) can be quantified. In this study, aSyn oligomers were generated after optimization of incubation conditions (pH, temperature, agitation, …). Then, different approaches were investigated to detect and follow the different species formed during the aggregation. We analyzed the oligomers by capillary gel electrophoresis (CGE) and SDS-PAGE. We found that capillary gel electrophoresis is a promising automated technique to analyze aSyn oligomers, due to the fact that it separates the aggregates according to their size, like the SDS-PAGE, but with more advantages. To gain sensitivity and selectivity by CGE, we used a laser-induced fluorescence detector. As aSyn do not have a native fluorescence, we derivatized it. After careful screening and optimization of various derivatization reagents, we could quantify with high sensitivity aSyn oligomers by CGE-LIF. We realized different calibration curves, and we had promising results that will allow us to quantify the different aSyn oligomeric forms in biological fluids. [less ▲]

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See detailA SPRAY DRYING METHOD FOR THE PREPARATION OF Na2FePO4F/CB AND Na2FePO4F/CNT COMPOSITES CATHODE FOR LITHIUM-ION BATTERIES
Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege; Brisbois, Magali et al

Poster (2017, September 18)

Fluorophosphates are considered among the most interesting series of cathode material for Li/Na-ion batteries1. Na2FePO4F (space group Pbcn), with its layered structure and two-dimensional pathways for ... [more ▼]

Fluorophosphates are considered among the most interesting series of cathode material for Li/Na-ion batteries1. Na2FePO4F (space group Pbcn), with its layered structure and two-dimensional pathways for facile Na+/Li+ transport, exhibits minimal structural changes upon reduction/oxidation. The average working voltage is 3.3 V vs. Li/Li+. Intercalation/deintercalation results in a volume change of only 3.7%. However, one of the key drawbacks of Na2FePO4F electrodes is their low intrinsic electronic conductivity. In order to study the effect of the carbon black and carbon nanotubes on the electrochemical performance of Na2FePO4F cathode material for lithium-ion batteries, Na2FePO4F, Na2FePO4F/CB and Na2FePO4F/CNT were prepared by a spray-drying method with different ratios of CB and CNT (10 and 20%). The crystal and local structure were analyzed by XRD and Mössbauer spectroscopy. The electrochemical properties were studied by galvanostatic cycling in lithium cells. The electrochemical performance is markedly better in the case of Na2FePO4F/CNT (20 wt%), with specific capacities of about 100 mAh/g (Na2FePO4F/CNT) at C/4 rate2 vs. 50 mAh/g for Na2FePO4F/CB. The characterization of Na2FePO4F/CB particles by electron microscopy revealed a carbon-poor surface and a good carbon dispersion for Na2FePO4F/CNT particles attributed to better diffusion of carbon nanotubes in the droplets during drying. References : 1-N. Eshraghi, S. Caes, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Electrochim. Acta, 228 (2017) 319–324. 2-M. Brisbois, S. Caes, M-T. Sougrati, B. Vertruyen, A. Schrijnemakers, R. Cloots, N. Eshraghi, R-P. Hermann, A. Mahmoud, F. Boschini, Solar Energy Materials & Solar Cells 148 (2015) 67-72. [less ▲]

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See detailSharing good practices in physical education to promote physical activity, wellness and health
Bonni, Jérémy ULiege; Cloes, Marc ULiege

Poster (2017, September 15)

The Wallonia-Brussels Federation is about to overhaul its education system, which will directly affect teacher training. Under the “Pact for Excellence in Education”, a group of experts – the Consortium ... [more ▼]

The Wallonia-Brussels Federation is about to overhaul its education system, which will directly affect teacher training. Under the “Pact for Excellence in Education”, a group of experts – the Consortium “Physical Activities, Well-Being and Health” – drawn from the 12 French-speaking institutions training PE teachers (universities and colleges) has been mandated by the Ministry of Education to design devices and tools aiming at implementing the new common core curriculum that is under construction. Good practices have been identified then described in information sheets common to the different consortiums of the PEE and listed in a table that cross-references the 6 dimensions of physical activity at school (Snyers et al., 2014) and the 20 objects of teaching-learning in health education (Turcotte et al., 2011). These good practices are meant to be shared later within a community of practice. This paper provides a description of one of these information sheets in order to illustrate the methodology applied in this project. [less ▲]

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See detailMusic in the Operating Room
XHAUFLAIRE, Gaël ULiege; Albert, Louisa; Gengoux, Monique

Poster (2017, September 15)

Listening to music during surgery has a subjective benefit to patients under local anesthesia. Its setting up during quiet routine oculoplastic surgery is easy and inexpensive. When subject to some ... [more ▼]

Listening to music during surgery has a subjective benefit to patients under local anesthesia. Its setting up during quiet routine oculoplastic surgery is easy and inexpensive. When subject to some elementary precautions, there is no increased risk. [less ▲]

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See detailExecutive functions in normal aging: the mediating effect of processing speed and attentional system
Gilsoul, Jessica ULiege; Simon, Jessica ULiege; Collette, Fabienne ULiege

Poster (2017, September 14)

Objective The normal aging is associated with decrease in executive functioning efficiency. However, the source of these cognitive difficulties could reside in some factors that are not executive per se ... [more ▼]

Objective The normal aging is associated with decrease in executive functioning efficiency. However, the source of these cognitive difficulties could reside in some factors that are not executive per se. Therefore, this present study is aimed at determining whether speed of processing and attentional variables would mediate the effect of age on the executive functioning. Participants and Methods Young (n = 104) and old (n = 63) participants were enrolled and carried out a large battery made of 21 executive (inhibition, shifting, working memory updating, and double task coordination), attentional (phasic alertness, selective attention, and sustained attention), and processing speed tasks. Based on Baron & Kenny’s recommendations (1986), mediation models of the age effect on the executive functioning were carried out with processing speed and attentional system taken as mediators. Results Selective attention significantly mediated the effect of age on working memory updating [􀇻R2 = 7%, p < .001]. Moreover, processing speed significantly mediated the effect of age on shifting [􀇻R2 = 7%, p < .001], working memory updating [􀇻R2 = 5%, p < .001], and double task coordination [􀇻R2 = 14%, p < .001]. Conclusions The mediating effect of age on most of the executive functions by measures of processing speed is in agreement with the literature showing a major influence of the slowdown of the processing speed on cognition (Salthouse, 1992; 2000; 2005). The age effect on inhibition did not seem to be mediated neither by the attentional variables nor the speed variables, which is in accordance with Zacks & Hasher’s hypothesis (1988). According to these authors, inhibition would be the first cognitive function to decline with age. [less ▲]

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See detailToward discovery of novel key genes for pancreatic beta cell regeneration in zebrafish
Carril Pardo, Claudio Andrès ULiege; Bergemann, David ULiege; Massoz, Laura ULiege et al

Poster (2017, September 14)

Diabetes is becoming a leading health issue worldwide. It occurs under insulin resistance/deficiency and when insulin-producing pancreatic beta-cell mass is dramatically reduced. Besides therapeutic ... [more ▼]

Diabetes is becoming a leading health issue worldwide. It occurs under insulin resistance/deficiency and when insulin-producing pancreatic beta-cell mass is dramatically reduced. Besides therapeutic strategies to preserve beta-cell mass and function and improve insulin treatments, beta-cell replacement constitutes a promising alternative to replenish the pancreas with functional beta-cells. Several observations of pancreatic cell plasticity has led to the hope that triggering beta-cell regeneration within the pancreas could be harnessed in future therapies. Still, mammals show limited regenerative capabilities, making difficult the study of these mechanisms. In contrast, zebrafish is extensively used for regeneration studies notably of beta-cells. We recently showed that the adult zebrafish ducts display characteristics of embryonic pancreatic progenitors that can give rise to beta-cells in physiological and induced diabetic condition*, and we determined the transcriptomic profile of zebrafish pancreatic ducts during beta-cell regeneration. To identify new genes crucial for beta-cell regeneration we plan to investigate the role of about 10 selected candidate genes by analyzing invalidating mutations created by CRISPR/Cas9. Their effect on beta-cell regeneration will be studied in 7-20 old larvae which we have established as experimental platform for functional studies. This project should help decipher the molecular mechanism of beta-cell regeneration. [less ▲]

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See detailFrom Metabolomics Study of Age-Related Macular Degeneration (AMD) to the Development of New Pyruvate Dehydrogenase Kinase Inhibitors (PDK)
Arslan, Deniz ULiege; Schoumacher, Matthieu ULiege; Pirotte, Bernard ULiege et al

Poster (2017, September 14)

Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population of industrialized countries. This blindness results from the deterioration of the macula, a small part of ... [more ▼]

Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population of industrialized countries. This blindness results from the deterioration of the macula, a small part of the retina specialized for the high-acuity vision. Exudative AMD, called “wet”, is characterized by the formation of new blood vessels growing under the retina according to a process named choroidal neovascularization (CNV). Currently, the aetiology and pathogenesis of AMD remain unclear. Nevertheless, a recent metabolomics study performed on the serum of “wet” AMD patients and on a CNV murine model, that mimics the effect of “wet” AMD, have demonstrated that lactate level is clearly involved in the severity of the pathology as well as the relationship between lactate, CNV and AMD [1]. According to this result, we suggest a new therapeutic approach of AMD based on the normalization of blood lactate level. The modulation of the lactate plasma concentration by treatment of the animals with synthetic compounds and more specifically Pyruvate Dehydrogenase Kinase (PDK) inhibitors significantly decrease the CNV. PDK and its four isoforms (PDK1-4) regulate the activity of the pyruvate dehydrogenase complex (PDH), a mitochondrial enzyme that plays a major role in the metabolic pathway of glucose, by reversible phosphorylation. Starting from these results, development of new PDK inhibitors could open the way to innovative treatment opportunities in AMD disease. Different analogues of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide (fig.1) have been already synthetized and pharmacological evaluation is currently in progress. According to the results obtained, various pharmacomodulations will be investigated. [less ▲]

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See detailUsing IPCAPS to identify fine-scale population structure
Chaichoompu, Kridsadakorn ULiege; Fentaw Abegaz, Yazew ULiege; Tongsima, Sissades et al

Poster (2017, September 09)

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See detailExploring insulin resistance in migraine: a population-based study
Streel, Sylvie ULiege; Donneau, Anne-Françoise ULiege; Schoenen, Jean ULiege et al

Poster (2017, September 09)

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See detailOptimization of hydroxyapatite synthesis via sol-gel process for bone reconstruction application
Tilkin, Rémi ULiege; Regibeau, Nicolas ULiege; Grandfils, Christian ULiege et al

Poster (2017, September 07)

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently ... [more ▼]

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently based on the realization of temporary porous matrices, also called "scaffolds". Scaffolds are highly porous matrices notably designed to structure the development of cells, but also to guarantee the function of the implant during the regeneration process. Several materials have been proposed for the conception of scaffold. These have to meet strict criteria regarding biocompatibility, degradability, mechanical and surface properties. As a result of their biomimetism, bioceramics, like hydroxyapatite (Ca5(PO4)3(OH)), have been widely developed during the past few years for bone reconstruction. The aim of this study is the optimization of the synthesis of hydroxyapatite by sol-gel process to be used in the conception of scaffold for bone reconstruction application. In this optic, powder obtained from two synthesis processes (wet precipitation and sol-gel process) were compared with commercial hydroxyapatite. For wet precipitation process, calcium nitrate and phosphoric acid were used as reagent. For sol-gel process, calcium acetate was used as the source of calcium and triethylphosphate as the source of phosphate. Reagents were mixed and the solution was aged. The powder was then dried and sintered. Finally, particles were washed in HCl to remove CaO and then dried. Those new materials were characterized, particularly in terms of chemical composition (XRD, FTIR), crystallinity (XRD), morphology (SEM, TEM), size (TEM, DLS) and Ca/P ratio (EDX). [less ▲]

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See detailAdvanced oxidative treatment for waste water contamined by pharmaceutical products
Belet, Artium ULiege; Vreuls, Christelle; Gillard, Nathalie et al

Poster (2017, September 07)

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See detailValidation of a TEOS based sol-gel coating for the protection of carbon fibres against oxidation regarding their use in metal matrix composites
Montrieux, Henri-Michel ULiege; Lecomte-Beckers, Jacqueline ULiege; Halleux, Jacques et al

Poster (2017, September 07)

The use of sol-gel coating on graphite substrates has sucessfuly been done. In particular, in the field of metal matrix composites, some authors state that the use and cure of TEOS sol-gel on carbon based ... [more ▼]

The use of sol-gel coating on graphite substrates has sucessfuly been done. In particular, in the field of metal matrix composites, some authors state that the use and cure of TEOS sol-gel on carbon based fibres may improve the liquid magnesium infiltration. The described mechanism is the lowering of the liquid/solid contact angle under some specific conditions of time and temperature by the occurrence of a metal-oxide exothermal chemical reaction. Furthermore the application of silica based sol-gels on carbon fibres in order to control the medium to high temperature oxidation of fibres preforms is less investigated. The primary objective is to understand more deeply the oxidation of some uncoated fibres by the comparison of ESEM observations to TGA/ATD curves. The secondary objective is to formulate and coat a TEOS based sol-gel on T300 carbon fibres and to evaluate the new oxidation behaviour. The ternary objective is to measure in a simple way the gain of oxidation resistance of some coated fibres. [less ▲]

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See detailCatalytic reforming of biomass gasification tars with bimetallic catalysts
Wolfs, Cédric ULiege; Claude, Vincent; Lambert, Stéphanie ULiege

Poster (2017, September 07)

The thermochemical method called “biomass gasification” is generating emphatic interest for the production of bio-Syngas (CO+H2) since this process presents the advantage of being renewable without ... [more ▼]

The thermochemical method called “biomass gasification” is generating emphatic interest for the production of bio-Syngas (CO+H2) since this process presents the advantage of being renewable without emitting CO2. However, in practical applications, there are still some technical problems due to high concentration of tars in the outlet gas, which can condensate and clog the pipes. Previous studies have highlighted the fact that the tar elimination via catalytic reforming seem to be the more practical and economical solution. Reforming catalysts present the following properties : a tailored mesoporous texture for the support and the high dispersion of active sites like metallic nanoparticles (Ni, Pd, Fe,…) [1]. In this work, Ni based alumina catalysts and doped with a second metal (Co, Cu, Fe, Mn, Mo) are synthesized by an aqueous sol-gel process. [less ▲]

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See detailLaterality patterns for Gestalts of language
Dumitru, Magdalena ULiege; Van Calster, Laurens ULiege; Bouffier, Marion ULiege et al

Poster (2017, September 07)

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See detailProtein-polysaccharide complexes, a tool for protein delivery in CaCO3 microparticles
Ramalapa, Bathabile ULiege; Crasson, Oscar ULiege; Vandevenne, Marylène ULiege et al

Poster (2017, September 06)

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions ... [more ▼]

INTRODUCTION The controlled delivery of proteins within calcium carbonate (CaCO3) particles is currently widely investigated. The success of these carriers has been driven by the ionic interactions between proteins and particles making the encapsulation of proteins highly dependent on the pH of reaction solutions and the isoelectric point of the protein.1 This poses a great limitation on the successful loading of proteins into microparticles. In this study, we explored the use of polysaccharide-protein interactions to strongly enhance the encapsulation of proteins in CaCO3 microparticles. EXPERIMENTAL METHODS Previously, Vandevenne and colleagues2 inserted a human chitin binding domain (ChBD) that has intrinsic affinity for hyaluronic acid (HA) into β-lactamase (BlaP). This generated chimeric protein, named BlaPChBD, was shown to be fully bifunctional. In this study this hybrid protein (BlapChBD) was associated to HA and successfully loaded into CaCO3 microparticles using super critical CO2 technology aided by the templating effect of HA on CaCO3. Furthermore, thrombin cleavage sites were engineered on both sides of the inserted ChBD in the chimeric BlaP so that release of the protein from the microparticles could be easily achieved by protease cleavage. The microparticles were characterised for size, surface charge, poly-morphism and protein loading and in-vitro release assays were performed. RESULTS AND DISCUSSION The presence of ChBD inserted into the β-lactamase increased the encapsulation of the protein by 6-fold when complexed with HA (Fig. 1). In addition, we also showed that the encapsulated BlaP remains stable during this process using kinetic reaction of β-lactam hydrolysis. Our data showed that vaterite CaCO3 microparticles of sizes ranging between 4 and 7 µm were produced. We were also able to demonstrate that thrombin cleavage increased the release of the protein from the microparticles within 36 hours from <25% to 87% (Fig. 2). In conclusion, the presence of ChBD successfully improved the encapsulation yield of the protein while retaining up to 81% of its activity. CONCLUSION Protein-polysaccharide complexation demonstrates an excellent approach for the delivery of sensitive biomacromolecules which can otherwise be complicated due to electrostatic hindrances. Future prospects include using the methods we have developed for encapsulation of therapeutic proteins and using calcium carbonate as a carrier and scaffold in bone regeneration for example. [less ▲]

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See detailEnvironmental impact of food waste treatment in an EcoCleaner, a portable accelerated composter
Groslambert, Sylvie ULiege; Léonard, Angélique ULiege; Finet, Sébastien

Poster (2017, September 06)

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially ... [more ▼]

Canteen and catering food wastes represent a challenge in waste management since they contain a large part of water and can be subject to biological processes during the waste management chain, especially during storage. Different solutions exist to manage this organic waste, for instance local and centralized composting, anaerobic digestion, or co-incineration with municipal waste. Except for local composting, theses methods need to collect and transport food waste to be processed, which mean transporting a lot of water. The Life Cycle in Practice (LCiP) (LIFE12 ENV/FR/001113) project helps SMEs to reduce the environmental impacts of their products and services across the entire life cycle. Within the frame of this project, the environmental impact of the Eco-Cleaner system is evaluated with the standardized life cycle assessment (LCA) methodology. Eco-Cleaner (EC) is a stand alone accelerated system that can transform food waste in valuable compost in only 24 hours. It is designed by Biowaste Recycling (http://biowasterecycling.com/). It can be declined in different capacities, from family size (about 1-2 kg/days of food waste) to large communities of 1300 place settings (up to 700 kg/days). EC technology is based on a permanent control of the ratio of humidity and temperature to optimize fermentation rate of food waste by a special consortium of more than thirty thermophilic bacteria. Waste reduction is about 90% and compost is sanitized to ensure the destruction of active parasites, insect eggs and germs. EC compost is dry (80-90% siccity level), and it has a quite high nitrogen content (about 3-4% on dry matter) and is rich in organic matter. Thanks to an activated carbon filter (replaced every two years), it is odourless and has no noxious gas emission. Since it's dry, this compost can be stored without further degradation, and its transport has a reduced environmental cost. The functional unit is the composting of one ton of food waste. The inventory includes the machine itself, its use, its refitting every twelve years, and its end of life. Refurbishing includes mainly the replacement of the motor, the heating resistance, the plastic parts, the seals, and only 5% of metals. Avoided chemical fertilizers consecutive to compost application are considered as system expansion, and it induces negative impact values in all categories. Electricity for use is the main impacting element in all categories (BE mix) but this impact can be reduced by the use of on-site photovoltaic panels. Abiotic depletion is highly impacted due to the metals the EC is made of (92% of total weight, principally steel), but a large improvement is achieved by the quadruplicating of the life span because of refurbishing, and the recycling of metals at the end of life. Specific process data are processed in SimaPro 8.1 software, using Ecoinvent 3.1 database for additional generic data, and analysed with the CML IA 3.03 method. [less ▲]

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See detailComparison of the electrochemical performance of Li4Ti5O12 spinel as negative electrodes for lithium-ion batteries prepared by sol gel and spray drying methods
Mahmoud, Abdelfattah ULiege; Piffet, Caroline ULiege; Berardo, Loris ULiege et al

Poster (2017, September 05)

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high ... [more ▼]

Energy is considered as the lifeblood of modern society. Rechargeable batteries are the most promising to meet the human needs concerning the energy storage thanks their high energy density and high energy efficiency. Most difficult challenges of the development of promising rechargeable batteries concern the electrode materials. Li4Ti5O12 (LTO) is one the most promising anode materials for Li-ion batteries, as it demonstrates very stable cycling stability and excellent safety. Its high operating potential (~1.5 V) allows to avoid the formation of SEI during the first cycle. The three-dimensional structure offers LTO excellent reversibility due to the near zero volume strain during the Li+ ion intercalation and deintercalation cycling. The main objective of this study on LTO samples was to evidence the effect of synthesis method and thermal conditions on their structural, morphological and electrochemical properties [1, 2]. The results demonstrate the strong influence of the synthesis route (Sol-Gel and spray-drying methods) and the thermal treatment on the capacity, cyclability and rate capability of the LTO spinel in Li-half-cell and Li-ion full-cell (see Figure 1). References [1] A. Mahmoud, J. M. Amarilla, K. Lasri, I. Saadoune, Electrochim. Acta 93 (2013) 163-172. [2] A. Mahmoud, J. M. Amarilla, I. Saadoune, Electrochim. Acta 163 (2015) 213-222. [less ▲]

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See detailSurface solar radiation modelling over 1900-2014: comparison between the regional climate model MAR and reanalyses
Wyard, Coraline ULiege; Doutreloup, Sébastien ULiege; Belleflamme, Alexandre ULiege et al

Poster (2017, September 04)

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere ... [more ▼]

Many studies show that the surface solar radiation has underwent large variations over the second half of the 20th century as a result of variations in cloud cover and aerosol loading in the atmosphere. However, it is difficult to build strong conclusions before the 1950s because of the observations scarcity. The evolution of the surface solar radiation has been reconstructed over 1900-2014 using the regional model MAR (« Modèle Atmosphérique Régional ») which has recently been chosen to be part of the EURO-CORDEX project, thanks to the CORDEX.be project. Simulations were performed at a horizontal resolution of 5 km over a domain of 600 x 550 km² covering Belgium. Boundary conditions were provided by four reanalysis products: ERA-interim (1979-2014) completed by the ERA40 (1958-1978), NCEP/NCAR-v1 (1948-2014), ERA-20C (1900-2010) and 20CRV2C (1900-2010). Surface solar radiation measurements from the Global Energy Balance Archive and cloud cover observations from Belgocontrol covering 1966-2007 were used for the evaluation of the MAR model and the forcing reanalyses. Results show that MAR produces much better results than the reanalyses. The driving reanalyses can generate divergent trends while they assimilate observations and are supposed to represent the same climate. [less ▲]

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See detailLCM of construction waste towards circular economy of buildings: VALDEM project
Roy, Aubin; Groslambert, Sylvie ULiege; Pasquet, Vanessa et al

Poster (2017, September 04)

From resource prospective, building and construction sector is responsible for more than third of global resource consumption, including 12% of the fresh water use and its generation of solid waste is ... [more ▼]

From resource prospective, building and construction sector is responsible for more than third of global resource consumption, including 12% of the fresh water use and its generation of solid waste is estimated to be 40% of the total waste volume. At European level, construction and demolition waste is the largest waste stream representing one third of all waste produced in EU. Therefore the resource efficiency and management is crucial in building construction. A very significant part of the Construction and Demolition Waste (CDW) is not recycled today. Also a very limited part is used as a recycled content in the construction and building products and materials. This lack is mainly due to heterogeneity and dispersion of waste flows decreasing efficiency and economic viability of recycling. To address this issue, VALDEM project (funded by Interreg FWVL European Fund) aims to overcome barriers to increase up-cycling applications. The project focuses its activities in North of France, Flanders and Walloon regions (Belgium), and stands out from usual approaches by its cross-border view of circular economy. VALDEM aims, on one hand, to optimize buildings end of life management by developing new deconstruction, sorting and recycling processes to produce uniform and accessible material flows. On the other hand, the project aims at increasing recycling and generating high quality secondary materials (concrete and other flows) to be used in future buildings within an up-cycling prospective. And finally it aims at validating the solutions from technical, scientific, economic and environmental point of view. The Environmental assessment, based on LCA, consists of identifying hotspots and key aspects to prioritize the efforts of different economic actors. As a first step, an LCA meta-analysis is conducted to provide an environmental picture for different potential activities within the scope of the project. As a next step, a comparative LCA is conducted to assess the environmental benefits and impacts of different solutions proposed in the framework of the project in a decision making context, and to limit the impact transfer and to generate the maximum value for all the stakeholders. Finally, results will be transferred to main actors (recycling operators, buildings contractors, product manufacturers …) in the three regions in order to consolidate future key aspects to eco-design a building in light of circular economy. [less ▲]

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See detailA Techno-Functional Study of the Aterian Technocomplex at Ifri n'Ammar
Tomasso, Sonja ULiege; Rots, Veerle ULiege

Poster (2017, September)

The tanged tools of the Aterian technocomplex have generally formed the central point in debates on early indications of hafting (Clark, 1970). However, in order to obtain a true understanding of the ... [more ▼]

The tanged tools of the Aterian technocomplex have generally formed the central point in debates on early indications of hafting (Clark, 1970). However, in order to obtain a true understanding of the specificity of this technocomplex, it is essential to study entire assemblages and understand the characteristics of and the relation between the tanged and non-tanged tool component, both on a technological and on a functional level. Until today no functional studies were yet undertaken on complete Aterian tool assemblages. We present the results of the technological and use-wear analysis of the non-tanged tool component of the Aterian assemblage of Ifri n’Ammar (Morocco) and focus on aspects of tool use and hafting. The rock shelter of Ifri n’Ammar, located in the eastern Moroccan Rif, is known for its rich stratified lithic assemblages, dating to 83 ± 6 ka to 130 ± 8 ka for the upper levels and to 145 ± 9 to 171 ± 12 ka for the lower levels (Richter et al., 2010). In the framework of the recently published dating results of other maghrebian sites (Dörschner et al., 2016), Ifri n’Ammar takes an important position within discussions on the chronological attribution of the Aterian: a large time span extended from MIS 6 to MIS 3 [3]. The sequence has provided a large diversity of tool morphologies which offer the possibility to contribute to the discussion about use and hafting of tanged and non-tanged tools. Aside from the tanged tools and foliates, the material from comparable Aterian assemblages can be defined by the presence of side scrapers, end-scrapers, denticulates, such as blades, bladelets or Levallois cores (Bouzouggar and Barton, 2012). Despite the frequent presence of post-depositional surface alterations, reliable functional interpretations could be identified on a large number of tools. Previous studies had already confirmed that the tanged tools were used while hafted for hunting and animal processing activities (Tomasso and Rots, 2017). Also a variety of tasks and gestures could be identified on the non-tanged tools based on a combination of diagnostic macro- and microscopic wear traces. Interestingly, also the non-tanged tool component shows evidence of hafting, indicating the existence of a variety in hafting techniques. The integration of the functional results on the non-tanged and tanged tool component allows addressing questions on tool variability, diversity in tool morphology and site function. We argue that the results highlight the importance of the site for improving our understanding of the North African MSA. [less ▲]

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See detailUltrasonic spray deposition of electrochromic oxide films
Maho, Anthony ULiege; Manceriu, Laura ULiege; Colson, Pierre ULiege et al

Poster (2017, September)

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See detailPlantes invasives dans les carrieres europeennes : quelle est la situation ?
Monty, Arnaud ULiege; Ortmans, William; Jorion, Alexis et al

Poster (2017, September)

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See detailSynthesis, characterization, and durability study of Pt-Co hollow nanoparticles deposited on carbon xerogel electrocatalysts for Proton Exchange Membrane Fuel Cells (PEMFC)
Zubiaur, Anthony ULiege; Asset, Tristan ULiege; Olu, Pierre-Yves et al

Poster (2017, September)

Proton exchange membrane fuel cell (PEMFC) catalysts are generally made of carbon black (CB)supported platinum-based nanoparticles. However, the properties of CBs, as described by RodríguezReinoso [1 ... [more ▼]

Proton exchange membrane fuel cell (PEMFC) catalysts are generally made of carbon black (CB)supported platinum-based nanoparticles. However, the properties of CBs, as described by RodríguezReinoso [1], are not optimal for electrocatalysis. A possible solution to the drawbacks of CBs is the use of synthetic nanostructured materials with a controllable and reproducible texture and with a pure, known and constant chemical composition; carbon xerogels (CX) exhibit such properties [2]. Recently, various highly dispersed CX-supported Pt nanoparticles catalysts (Pt/CX) have been synthesized via several methods, such as the strong electrostatic adsorption (SEA) [3] or the charge-enhanced dry impregnation (CEDI) [4]. However, the decrease of the Pt mass contained in the PEMFC electrodes, in particular at the cathode where the oxygen reduction reaction (ORR) is processed, remains a major challenge. Improving the ORR mass activity is currently best achieved by alloying Pt with 3d-transition metal atoms such as cobalt (Co). These alloys perform better than the ORR because the substitution of some Pt atoms by 3d-metal atoms with smaller radius leads to a modified Pt electronic structure [5]. The synthesis and durability of Pt-Co hollow particles/CX (Fig. 1) is currently being studied in our groups. The results indicate that the specific and mass activity of the bimetallic hollow particles dispersed on CX is ca. 10 times higher than that of pure Pt/CB. The first durability results show good stability of the hollow particles structure. Performances and accelerated stress tests (ASTs) in PEMFCs were finally performed. [less ▲]

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See detailCould indolic glucosinolates root profiling be correlated to Brassica napus L. cadmium stress tolerance?
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

Poster (2017, September)

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary ... [more ▼]

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary metabolites hugely produced within Brassicaceae family can interact in regards to cadmium stress as well. Sulfate primary metabolism plays an important role to Brassica napus L. cadmium stress tolerance and glucosinolates could serve as sulfate pool under such stress conditions. We describe here an in vitro experiment performed under sterile and controlled conditions showing the relationship between Brassica napus L. var. Es Astrid tolerance to a low concentration of cadmium and a decrease of a major indolic glucosinolates produced by the roots of this variety. Phenotyping results obtained from these cadmium stress conditions confirm also that Brassica napus L. remains a highly cadmium tolerant species probably due to glucosinolates root metabolism particularly. [less ▲]

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See detailDefeatist performance beliefs in a sample of individuals with persistent negative symptoms
Thonon, Bénédicte ULiege; Li, Xueying; Velligan, Dawn

Poster (2017, September)

Objective Negative symptoms of schizophrenia, in particular motivational deficits, have showed to be linked to dysfunctional attitudes, such as defeatist performance beliefs. This relationship has been ... [more ▼]

Objective Negative symptoms of schizophrenia, in particular motivational deficits, have showed to be linked to dysfunctional attitudes, such as defeatist performance beliefs. This relationship has been found in various types of samples but has not been investigated in samples of individuals presenting persistent negative symptoms (PNS). The present study aimed to assess the relationship between defeatist performance beliefs and negative symptoms in a sample of individuals diagnosed with schizophrenia and PNS. Another objective was to evaluate the link between change in defeatist beliefs and change in negative symptoms, before and after psychosocial intervention. Methods Fifty-one participants diagnosed with schizophrenia and PNS followed 9 months of Motivation and Engagement Training. They were assessed before and after the intervention using the Negative Symptom Assessment (NSA), the Brief Negative Symptom Scale (BNSS), the Clinical Assessment Interview for Negative Symptoms (CAINS), and de the Defeatist Performance Beliefs scale. Results At baseline, defeatist performance beliefs were not significantly associated with negative symptoms, except with the motivation factor of the BNSS, but only at the trend level. However, change in defeatist beliefs was moderately correlated with change in motivation, as measured with the BNSS, but not with the other measures. Conclusion These results underlie the necessity of broadening the targets of psychosocial interventions to factors related to negative symptoms other than dysfunctional attitudes. [less ▲]

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See detailA technological and functional examination of the Aurignacian end-scrapers from Grotta di Fumane
Aleo, Alessandro; Duches, Rossella; Rots, Veerle ULiege et al

Poster (2017, September)

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See detailDietary inulin supplementation promotes weight loss in obese individuals
Hiel, Sophie; Rodriguez, Julie; Gianfrancesco, Marco ULiege et al

Poster (2017, September)

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See detailNew filamentous mutant of Yarrowia lipolytica and its use in biofilm bioreactors
Vandermies, Marie ULiege; Kar, Tambi ULiege; Denies, Olivia ULiege et al

Poster (2017, September)

The non-conventional yeast Yarrowia lipolytica is widely investigated for its unusual metabolic properties. Among them is the ability of Y. lipolytica to adopt an ovoid or hyphal morphology according to ... [more ▼]

The non-conventional yeast Yarrowia lipolytica is widely investigated for its unusual metabolic properties. Among them is the ability of Y. lipolytica to adopt an ovoid or hyphal morphology according to environmental conditions. The mechanism of dimorphic transition involves numerous genes, which have been poorly documented to date. Here, we report on the isolation of a filamentous mutant from an insertion mutagenesis library, the subsequent identification of the mutated gene, and the use of this filamentous mutant in biofilm bioreactors. [less ▲]

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See detailUseful tools for genome editing in the non-conventional yeast Yarrowia lipolytica
Vandermies, Marie ULiege; Denies, Olivia ULiege; Nicaud, Jean-Marc et al

Poster (2017, September)

The non-conventional yeast Yarrowia lipolytica is increasingly used for the production of recombinant proteins or biomolecules with biotechnological or pharmaceutical applications. The development of such ... [more ▼]

The non-conventional yeast Yarrowia lipolytica is increasingly used for the production of recombinant proteins or biomolecules with biotechnological or pharmaceutical applications. The development of such a cell factory requires steps of genome editing that rely on selectable markers. The recently identified EYK1, encoding erythrulose kinase, can serve as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Besides, the cloning-free strategy developed here simplifies the construction of disruption cassettes for genome editing in Y. lipolytica. [less ▲]

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See detailPromoting "health literacy friendly" healthcare institutions
Henrard, Gilles ULiege

Poster (2017, September)

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See detailRecycling of silicon used in solar cells to prepare silicon-carbon composite powders as anode material in li-ion batteries
Eshraghi, Nicolas ULiege; Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege et al

Poster (2017, September)

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as ... [more ▼]

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as an electrode material for lithium or sodium batteries. The main technological challenge associated with the use of silicon in this type of application is to control the volume expansion during charge/discharge cycles. This problem could be solved through the synthesis of Silicon/carbon composites in which the size of the silicon particles and their dispersion must be controlled [1–4]. We develop a carbon matrix consisting of graphene or carbon nanotubes (CNT) that allow the incorporation of silicon particles coated with a carbon layer (Si@C/C). The process is divided in two main steps. In the first step, Si wafer pieces are ground to prepare Si powder and then a mixed suspension of Si and an organic carbon source (Acetic acid, Ascorbic acid or Lactose) is spray-dried followed by heat treatment to generate the coverage of silicon particles with carbon (Si@C). In the second step, aqueous suspension of Si@C and graphene/CNT is spray dried and heat treated to obtain the final composite structure. The morphology of composite materials is analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Electrochemical performance of Si@C/C composites are characterized by galvanostatic charge-discharge cycling and electrochemical impedance spectroscopy (EIS). [less ▲]

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See detailPreparation and characterization of Silicon-carbon composite powders using recycled Silicon from solar cells as anode material in Li-ion batteries
Eshraghi, Nicolas ULiege; Mahmoud, Abdelfattah ULiege; Berardo, Loris ULiege et al

Poster (2017, September)

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as ... [more ▼]

Currently, silicon (Si) coming from the recycling of solar cells is a non-valued fraction. The principal aim of this work is the development of silicon recovered during the dismantling of solar panels as electrode material for Li-ion batteries. The main technological challenge associated with the use of silicon in this type of application is to control the volume expansion during charge/discharge cycles. This drawback could be avoided through the formation of Silicon/carbon composites in which the size of the silicon particles and their dispersion must be controlled. In this research, we develop a carbon matrix consisting of graphene or carbon nanotubes (CNT) that allow the incorporation of silicon particles coated with a carbon layer (Si@C/C). The process is divided in two main steps : I) the grinding of leached Si wafer pieces in order to extract pure Si powder and then a mixed aqueous suspension of this recycled Si and an organic carbon source (Acetic acid, Ascorbic acid or Lactose) is spray-dried followed by heat treatment to generate the coating of silicon particles with carbon (Si@C). Then, II) aqueous suspension of Si@C and graphene/CNT is spray dried and calcined to obtain the final composite structure (Si@C/C). The morphology of composite materials is analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Electrochemical performance of Si@C/C composites are characterized by galvanostatic charge-discharge cycling and electrochemical impedance spectroscopy (EIS). [less ▲]

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See detailFlowering roots: Insensitive Root Growth 1 contributes to photoperiod-induced root responses in Arabidopsis.
Orman-Ligeza, Beata ULiege; Detry, Nathalie ULiege; Tocquin, Pierre ULiege et al

Poster (2017, September)

The capacity to perceive and respond to seasonal changes of day length is essential for flowering plants. Under favourable photoperiod, a mobile stimulus synthesized in leaves moves to the shoot apex and ... [more ▼]

The capacity to perceive and respond to seasonal changes of day length is essential for flowering plants. Under favourable photoperiod, a mobile stimulus synthesized in leaves moves to the shoot apex and triggers the expression of genes required for the transition to flower initiation. Although transition from vegetative to reproductive state also encompasses a transcriptional response in roots, the internal signalling pathways and how root system architecture adjusts to this changing status remain elusive. Here we show in Arabidopsis that root growth rate increases upon a transfer to flowering-inductive long days while remaining constant under short days. To elucidate genetic components of this response, we performed a meta-analysis of available root-growth and flowering-related arrays and selected genes with overlapping transcriptional profiles for further analyses. Loss of function in a member of the basic leucine zipper transcription factor gene family, hereafter named Insensitive Root Growth-1 (IRG1), was found to suppress photoperiod-response of root growth with no defect in flowering time. We show that sucrose, but neither glucose nor mannitol in the growth medium under long days, is needed to trigger this response. In addition, extending the photoperiod with non-photosynthetic far red light had no effect on root growth of irg-1 mutant, alike wild type Col-0. The expression level of IRG1 in the roots remains low during the daytime and peaks late at night, suggesting that this gene is regulated by the clock’s evening loop. Taken together, our results suggest that IRG1 may be involved in sucrose-mediated stimulation of root growth during the night phase in Arabidopsis. The functional characterisation of IRG1 is currently underway. [less ▲]

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See detailTowards understanding the function of JOINTLESS gene in tomato inflorescence
Huerga Fernandez, Samuel ULiege; Gómez Roldán, Maria Victoria; Orman-Ligeza, Beata ULiege et al

Poster (2017, September)

The lack of fruit abscission is a trait of great agronomical value. In tomato, the jointless phenotype, referring to the lack of abscission zone (AZ) in the flower pedicel, has been obtained by two ... [more ▼]

The lack of fruit abscission is a trait of great agronomical value. In tomato, the jointless phenotype, referring to the lack of abscission zone (AZ) in the flower pedicel, has been obtained by two independent mutations, named jointless (j) and jointless-2 (j-2). The corresponding genes encode MADS-box transcription factors, as shown in 2000 for J (Mao et al. 2000) and very recently for J-2, known as SlMBP21 (Gomez-Roldan et al., 2017). Similar to the quartet model of MADS-box protein complexes regulating floral organ formation, J and J-2 interact with MADS-box partners, among which MACROCRALYX (MC), to regulate AZ formation (Liu et al. 2014). In addition to - or in connection with - AZ formation, J acts during the building of the inflorescence to regulate meristem fate. Indeed j mutants produce leafy inflorescences characterized by faster flower maturation and resumption of vegetative meristems (Périlleux et al. 2014). For these traits, j is epistatic to j-2. The involvement of J in the regulation of meristem fate is consistent with the roles of its closest homologs in Arabidopsis, AGAMOUS LIKE 24 (AGL24) and SHORT VEGETATIVE PHASE (SVP). Our goal is to identify J targets in order to unravel its multiple functions in the tomato inflorescence. [less ▲]

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See detailVINYLIC COPOLYMERS FOR PH-SENSITIVE LIPID NANOCAPSULES
Pautu, Vincent ULiege; Lepeltier, Elise; Debuigne, Antoine ULiege et al

Poster (2017, September)

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See detailUp-scalable spray-drying synthesis of Na2Ti3O7
Piffet, Caroline ULiege; Vertruyen, Bénédicte ULiege; Mahmoud, Abdelfattah ULiege et al

Poster (2017, September)

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See detailTargeted mutagenesis of orphan GPCRs of the SREB family
Laschet, Céline ULiege; Dupuis, Nadine ULiege; Geubelle, Pierre ULiege et al

Poster (2017, September)

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See detailLexical Diachronic Semantic Maps
Georgakopoulos, Athanasios ULiege; Polis, Stéphane ULiege

Poster (2017, September)

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