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See detailRisk of emergence of a hyperpathogenic bovine leukemia virus by mutation of a single envelope N-linked glycosylation site
De Brogniez, Alix ULg; Bouzar, Amel-Baya; Jacques, Jean-Rock ULg et al

Poster (2015, February 11)

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is ... [more ▼]

- Introduction : Pathogens have co-evolved with their host to ensure efficient replication and transmission without inducing excessive pathogenicity that would indirectly impair their persistence. This is exemplified by the bovine leukemia virus (BLV) system in which lymphoproliferative disorders develop in ruminants after latency periods of several years. Infection of sheep and cattle with BLV is a model system for the related human T-lymphotropic virus type 1 (HTLV-1) responsible for Adult T-cell Leukemia (ATL). - Aims : The goal of this work is to investigate the role of N-glycans of the viral envelope protein during viral replication and pathogenesis. - Methods and results : Using glycosylation inhibitors and lectins, we showed that N-glycosylation is involved in viral infection (i.e. cell-to-cell fusion). Using reverse genetics of an infectious molecular provirus, we next demonstrated that a particular N-linked envelope glycosylation site (N230) limits viral replication and pathogenicity in vitro and in vivo. We have thus generated a viral mutant that is more pathogenic than the wild type strain. - Conclusions : To our knowledge, this is the first time that a hyperpathogenic BLV has been identified. This unexpected observation has important consequences in terms of disease control and managing. Indeed, during evolution, pathogens and their hosts should achieve an equilibrium allowing the coexistence of the two species. Occurrence of this particular mutation may thus represent a potential threat associated with emergence of hyperpathogenic BLV strains and possibly of new variants of the related HTLV-1. [less ▲]

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See detailÉtude du Typus mélancholicus au sein d’une population de dépressifs unipoalires
ramackers, Amélia; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailÉtude du récit de vie de l’adulte à haut potentiel
Mormont, Elodie; Gauthier, Jean-Marie ULg; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailLa personnalité Borderline à travers le test de Rorschach
Rommes, Jennifer; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailAn original solver for spillway flow
Goffin, Louis ULg; Erpicum, Sébastien ULg; Dewals, Benjamin ULg et al

Poster (2015)

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See detail"Gérer ses émotions et agir : Pleine conscience et ACT" : Etude préliminaire de l'efficacité d'un groupe thérapeutique
Wagener, Aurélie ULg; Dierickx, Christophe; Herbeuval, Sandrine et al

Poster (2014, December 16)

Introduction : L’apprentissage de la gestion des émotions est une demande très fréquente en psychothérapie. Les techniques issues des programmes de pleine conscience et des thérapies d’acceptation et ... [more ▼]

Introduction : L’apprentissage de la gestion des émotions est une demande très fréquente en psychothérapie. Les techniques issues des programmes de pleine conscience et des thérapies d’acceptation et d’engagement ont notamment pour objectif de guider les clients dans cet apprentissage. Afin de répondre à cette demande, nous avons mis en place un groupe « Gérer ses émotions et agir : pleine conscience et ACT » et mené une recherche-action afin d’en tester l’efficacité. Méthodologie : Le cycle thérapeutique comportait 3 séances (3x3 heures) sur un délai de 6 semaines et 2 temps d’évaluation (avant la première séance [T0] et après la dernière [T1]). Les évaluations adminis-trées aux différents temps concernaient les données socio-démographiques, l’humeur, le niveau de pleine conscience, la flexibilité psychologique, le niveau d’engagement dans des activités, la mo-tivation et le sentiment d’auto-efficacité. Résultats : Cinq adultes (M âge = 37,46; ET = 7,96) ont participé à ce premier cycle. Les analyses prélimi-naires indiquent un effet significatif du facteur temps (N = 5). Ainsi, une augmentation du niveau d’engagement dans des activités (F(1) = 20,07, p = 0,01), de la motivation (F(1) = 15,00, p = 0,02) et du sentiment d’auto-efficacité (F(1) = 17,48, p = 0,02) est observée entre le T0 et le T1. Conclusion : Les résultats préliminaires semblent indiquer que les participants au groupe thérapeutique tirent des bénéfices de la participation à ce cycle thérapeutique. La récolte des données et la constitution d’un groupe contrôle se poursuivent. Les résultats seront plus longuement discutés lors du congrès. [less ▲]

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See detailAnxiété et dépression chez l'enfant parentifié. Mieux identifier pour mieux intervenir.
Haxhe, Stéphanie ULg; Stassart, Martine ULg; D'Amore, Salvatore ULg

Poster (2014, December 16)

La parentification reste un phénomème mal connu. Pourtant, les conséquences pour l'enfant sont à prendre au sérieux.

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See detailRuzagayura, la grande famine du Rwanda moderne
Singiza, Dantès ULg

Poster (2014, December 16)

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See detailVariability of Jupiter’s Main Auroral Emission in Response to Magnetospheric Hot Plasma Injections
Badman, Sarah; Bonfond, Bertrand ULg; Fujimoto, Masaki et al

Poster (2014, December 16)

We present observations of Jupiter’s FUV aurora acquired by the Hubble Space Telescope during a two-week interval in January 2014. The variability of the main auroral emission was studied using ... [more ▼]

We present observations of Jupiter’s FUV aurora acquired by the Hubble Space Telescope during a two-week interval in January 2014. The variability of the main auroral emission was studied using latitudinal profiles of intensity. The main oval intensity was found to be reduced when bright patches of diffuse emission were present at lower latitudes. These low latitude emissions are interpreted as the signatures of hot plasma injections from the outer magnetosphere, a process which has previously been related to interchange between the flux tubes from the outer magnetosphere and outward-moving flux tubes loaded with iogenic plasma. The main emission was also observed to broaden and shift in latitude, and occasionally display a double peak structure. These observations are interpreted with reference to the expected changes in auroral field-aligned currents associated with the replacement of the radially-stretched, mass-loaded flux tubes in the middle magnetosphere by more dipolar flux tubes containing rarefied hot plasma. [less ▲]

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See detailWhen microplastic is not microplastic: ingestion of artificial cellulose fibers by macrofauna living in seagrass macrophytodetritus
Collard, France ULg; Remy, François ULg; Gilbert, Bernard ULg et al

Poster (2014, December 12)

Vagile macroinvertebrates associated with Posidonia oceanica exported litter were sampled in August 2011, November 2011 and March 2012 in the Calvi Bay (Corsica), near the STARESO oceanographic station ... [more ▼]

Vagile macroinvertebrates associated with Posidonia oceanica exported litter were sampled in August 2011, November 2011 and March 2012 in the Calvi Bay (Corsica), near the STARESO oceanographic station. Contents of digestive tracts were analyzed and fibers of various sizes and colors were found. Fibers were found in 27.6% of the digestive tracts in the nine dominant species. No correlation was found between number of fibers and taxonomic or trophic level. There were no seasonal or spatial preferences and thus we hypothesize that the organisms ingest these fibers randomly throughout the year. Analyses performed with a Raman spectroscope showed that these fibers were composed of cellulose associated with a coloring agent following the fiber color. Red fibers were dyed with the Direct Red 28, blue fibers were dyed with Direct Blue 22. Analyses by a scanning electron microscope (SEM) showed that cellulose fibers had the particular morphology of artificial cellulose fibers called: viscose. Our SEM analyses were compared to literature. This comparison assessed that fibers found in digestive tracts were made of viscose. In a first approach, viscose fibers looked like microplastic fibers because of their color and shape. However, it appeared that these fibers were made of artificial cellulose which is very different than plastic in terms of impacts and fate in the organisms. This study highlights the importance of physico-chemical analyses such as Raman spectroscopy and SEM to certainly identify the composition of particles ingested by organisms. From an ecological point of view, the red coloring agent is known to be carcinogenic in mammals and fish. Consequently, this pollution could provoke an environmental problem for the P. oceanica litter vagile macrofauna. [less ▲]

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See detailA morphospace for the marine angelfishes (Pomacanthidae): patterns of diversity
Frederich, Bruno ULg

Poster (2014, December 12)

The Pomacanthidae (marine angelfishes) is an iconic reef fish family of about 88 species. They have a circum-global distribution on tropical to warm-temperate reefs. Marine angelfishes occupy a diverse ... [more ▼]

The Pomacanthidae (marine angelfishes) is an iconic reef fish family of about 88 species. They have a circum-global distribution on tropical to warm-temperate reefs. Marine angelfishes occupy a diverse range of trophic niches, ranging from spongivory and algivory to zooplanktivory. Previous morpho-functional analysis of this family mainly focused on the head region and gut morphology in a limited number of species. However a morphological study including a large sample of angelfish representatives is currently lacking. Here, I explore the diversity of body morphology in this reef fish group. I collected x-ray images of 228 museum specimens from 71 species and I used landmark-based geometric morphometrics to quantify the overall body shape variation within this family. In a test for interspecific allometry, the linear regression of shape variables onto size (LogTL; TL = total length) was significant (P < 0.001). However, the percentage of explained variance in this model (23%) showed no strong relation between size and body shape. Main shape variation across species was explored using a principal component analysis on shape variables. The two main axes (PC1 and PC2) explained 74% of the total variance of the dataset. Pomacanthus spp., which mainly feed on fixed invertebrates (sponges and tunicates), have deep body with high and angular cephalic profile (lowest values along PC1). Conversely, the zooplanktivorous Genicanthus spp., those have a more pelagic lifestyle, show a slender body with shorter anal fin (highest values along PC1). The pigmy angelfishes from the genus Centropyge, which group different diets (omnivory or algivory), show a more robust and rectangular body shape (high scores along PC2). This work provides the row data for future studies dealing with the mode of phenotypic diversification of Pomacanthidae during evolution. [less ▲]

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See detailRapid morphological change of barbels (Cyprinidae) after the dry-up of Sahara
Brahimi, Amina; Tarai, Nacer; Libois, Roland ULg

Poster (2014, December 12)

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See detailStable isotope ratios reveal trophic niche partitioning among hermit crabs from tropical polyspecific seagrass meadows
Michel, Loïc ULg; Frederich, Bruno ULg; Lavitra, Thierry et al

Poster (2014, December 12)

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local ... [more ▼]

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local populations. Meadows however undergo multiple threats linked to human activities (increased nutrient input, overfishing, invertebrate overharvesting, etc.). It is currently hard to assess how seagrass meadows could respond to anthropogenic impacts due to poor knowledge of their functional ecology. In an effort to unravel trophic interactions ruling the food webs associated to seagrass beds of the Toliara Great Reef (SW Madagascar), we studied resource segregation between two common Diogenidae hermit crabs (Dardanus scutellatus and Ciliopagurus tricolor) using stable isotope ratios. Interspecific differences were noted in isotopic composition of carbon (δ13C = -12.22 ± 1.73 ‰ for D. scutellatus, δ13C = -14.55 ± 0.73 ‰ for C. tricolor), nitrogen (δ15N = 4.73 ± 0.53 ‰ for D. scutellatus, δ15N = 5.20 ± 0.61 ‰ for C. tricolor) and sulfur (δ34S = 14.08 ± 2.32 ‰ for D. scutellatus, δ34S = 16.73 ± 1.49 ‰ for C. tricolor), suggesting that the two species do not feed on the same items. In addition, SIBER (Stable Isotope Bayesian Ellipses in R) modeling based on C and N data clearly showed that no overlap was present in the core isotopic niches of the two species. It also indicated that the isotopic niche of D. scutellatus was greater than the one of C. tricolor, implying that the former feeds on a greater number of items than the latter. While hermit crabs are generally considered as omnivorous species, this study highlighted differences in the foraging ecology of D. scutellatus and C. tricolor. These differences could help to limit competition for food between these two species, and facilitate their coexistence in Malagasy seagrass beds. [less ▲]

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See detailSound production in piranhas and relatives: preliminary results
Melotte, Geoffrey ULg; Michel, Christian ULg; Parmentier, Eric ULg

Poster (2014, December 12)

Acoustic communication plays an important role in the life of many teleost species where it is mainly involved in agonistic and/or courtship behaviour(s). Despite the large number of species in the family ... [more ▼]

Acoustic communication plays an important role in the life of many teleost species where it is mainly involved in agonistic and/or courtship behaviour(s). Despite the large number of species in the family Serrasalmidae (92 species), sound production has been described only in some of them, particularly in the genera Serrasalmus and Pygocentrus. The aim of this study is to investigate the sound producing abilities of different Serrasalmidae species and to understand the corresponding mechanisms. Two herbivorous species of Serrasalmidae, Piaractus brachypomus and Metynnis lippincottianus, produce sounds composed of a single pulse. The mechanism involved in these species is not yet understood. In contrast, the calls emitted by several species of Serrasalmus and Pygocentrus are harmonic sounds composed of several pulses without inter-pulse interval. Their mechanism results from the forced vibration of the swimbladder following the contraction of sonic muscles that are attached to tendons surrounding ventrally the bladder. Another species, Pygopristis denticulata, is able to produce two types of sounds. The first sound consists of several pulses with irregular pulse period and is likely produced by a sonic muscle inserting on the skull and on the rostral part of the swimbladder. The second sound is multi-pulsed and, contrary to all other sounds described here, possesses a high dominant frequency suggesting a mechanism that does not involve the swimbladder and that remains to be determined. According to these results, Serrasalmidae seems to contain many species able to produce sounds by means of different mechanisms. [less ▲]

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See detailL'Aurignacien de la grotte Yafteh et son contexte
Otte, Marcel ULg; Flas, Damien ULg; Zwyns, Nicolas et al

Poster (2014, December 06)

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See detailDetecting microbial patterns in relation to soil agricultural practices and the plant development stage
Degrune, Florine ULg; Dufrêne, Marc ULg; Taminiau, Bernard ULg et al

Poster (2014, December 02)

Agricultural practices have a strong impact on soil bacteria and fungi. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these ... [more ▼]

Agricultural practices have a strong impact on soil bacteria and fungi. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these effects in relation to changes induced by agriculture and plant stage in soil conditions. Some bacteria are influenced only by the plant stage, which induces changes in soil humidity, pH, nitrates, and carbon. We would thus expect these bacteria to be highly sensitive to these parameters. Other bacteria are affected only by the tillage practice applied. Further study is needed to identify the soil parameters responsible for this effect. The plant stage also has a great impact on fungal community composition. [less ▲]

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See detailMid-winter freeze experiment in the Arctic Ocean: Norwegian Young sea ICE cruise (N-ICE2015)
Nomura, Daiki; Granskog, Mats A.; Fransson, Agneta et al

Poster (2014, December 02)

In mid-January 2015, RV Lance will freeze into the ice north of Svalbard, Arctic Ocean at around 83.25°N 30°E, and passively drift with the ice as part of the Norwegian Young sea ICE cruise (N-ICE2015 ... [more ▼]

In mid-January 2015, RV Lance will freeze into the ice north of Svalbard, Arctic Ocean at around 83.25°N 30°E, and passively drift with the ice as part of the Norwegian Young sea ICE cruise (N-ICE2015). Judging from historic sea ice drift trajectories, it is likely that RV Lance will drift in a SW direction and the ship will probably be freed from the ice in mid spring after about two to three months of drift. Thereafter, RV Lance will return to her starting position and start a new drift. Under all circumstances, the ice drift project will end in late June 2015. Throughout the cruise the focus will be on the interaction of the atmosphere-ice-ocean system and the response of the marine ecosystem to the thinner ice regime. The overall goal of the project team is to improve our understanding of the role of the younger ice pack in the Arctic on greenhouse gas fluxes and to ultimately assess whether the Arctic Ocean is a sink or source of greenhouse gases. We plan to conduct long-term synchronous observations of Arctic snow and sea ice biogeochemistry and physics and fluxes of carbon dioxide, methane, nitrous oxide and bromoform. This work targets at filling a crucial gap in our understanding of the role of Arctic sea ice in the climate system. This is done by conducting state of the art observations on Arctic sea ice in the polar night, when observations are basically non-existent. Further we are focusing on the new thinner ice regime, which is even less documented. We aim to understand how the thinner sea ice in the Arctic basin contributes (i) to important greenhouse gas exchange between the atmosphere and ocean and (ii) to aerosol formation, that contribute to the radiative balance of the planet. This work will increase direct collaboration between Japanese and European scientists in the Arctic, and combines complimentary expertise and experience from several international partners to carry out the interdisciplinary work proposed. [less ▲]

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See detailMOTOR CONTROL AND LOW BACK PAIN IN ELITE SOCCER PLAYERS
GROSDENT, Stéphanie ULg; Demoulin, Christophe ULg; Namurois, Guy et al

Poster (2014, December)

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See detailPoster session 6: Saturday 6 December 2014, 08:30-12:30Location: Poster area.
Henri, C.; DULGHERU, Raluca Elena ULg; Magne, J. et al

Poster (2014, December)

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See detailStigmergy as a mechanism to produce collective vortex behaviours: a study case in shoveler duck
Delcourt, Johann ULg; bode, W. Nikolaï

Poster (2014, December)

Ant mill, caterpillar circle, bat donut, bacteria vortex, duck swirl and fish torus are different names for rotating circular formations of animals, where individuals turn around a common centre. Even if ... [more ▼]

Ant mill, caterpillar circle, bat donut, bacteria vortex, duck swirl and fish torus are different names for rotating circular formations of animals, where individuals turn around a common centre. Even if the ubiquity of this behavioural phenomenon might have suggested common causes or fundamental underlying principles across contexts, a variety of proximate mechanisms can give rise to vortex behaviours. Here, we investigate if stigmergic process (mechanism of self-organisation wit hout direct communication or interaction between individuals) is able to produce different collective behaviours, notably collective vortices. We present an individual-based simulation model for the movement of populations in a resource landscape that allows us to vary the strength of the interactions mentioned above. The key assumption and novelty of our model is that individuals can cause the release of additional nutrients, as well as consuming them. Our model produces clear predictions. For example, we expect more tortuous individual movement paths and higher levels of aggregation in populations occupying homogeneous environments where individual movement makes more nutrients available. We also show how observed movement dynamics could change when local nutrient sources are depleted or when the population density increases. Our predictions are testable and qualitatively reproduce the different feeding behaviours observed in filter-feeding ducks (Anas clypeata), for example. We suggest that considering two-way interactions between feeding individuals and resource landscapes could help to explain fine-scale movement dynamics. [less ▲]

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See detailPoster session 1: Wednesday 3 December 2014, 09:00-16:00Location: Poster area.
Romano, G.; D'ancona, G.; Pilato, G. et al

Poster (2014, December)

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See detailEcological diversity of damselfishes (Pomacentridae) from Moorea
Gajdzik, Laura ULg; Parmentier, Eric ULg; Lepoint, Gilles ULg et al

Poster (2014, December)

The Pomacentridae (damselfishes) represents the 3rd most species-rich coral reef fish family, including 394 species spread worldwide. Recent studies suggested that damselfishes iteratively evolve along ... [more ▼]

The Pomacentridae (damselfishes) represents the 3rd most species-rich coral reef fish family, including 394 species spread worldwide. Recent studies suggested that damselfishes iteratively evolve along three main trophic guilds: “the pelagic feeders” feeding mainly on zooplankton, “the benthic feeders” grazing filamentous algae or biting coral polyps, and an “intermediate” group with species feeding on zooplankton, small benthic invertebrates and algae in variable proportions. Comparing the ecological diversity of damselfish communities among various geographical regions could assess such an iterative radiation. Within this context, the present study is a first attempt exploring the trophic diversity of damselfish community at Moorea Island (French Polynesia). The relationship between the trophic guilds, their habitat diversity and their behavior is evaluated. Habitat and behaviour of 16 damselfish species were described during transects along which different kinds of data were collected. For examples, the location within the reef (e.g. fringing or barrier reef), the depth, the substrate over which fishes are living or the solitary/gregarious habits were recorded. Diet of individuals was investigated by means of stomach content and stable isotopes analyses. Correlations between the dietary preferences and ecological data (habitat and behaviour) are given and the ecological diversity among trophic groups is compared. [less ▲]

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See detailTrace element kinetics in caged Mytilus galloprovincialis
Richir, Jonathan ULg; Lepoint, Gilles ULg; Donnay, Annick et al

Poster (2014, December)

Trace elements (TEs) remain contaminants of concern because of their persistence, ability to concentrate in organisms and toxicity. The Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 is a ... [more ▼]

Trace elements (TEs) remain contaminants of concern because of their persistence, ability to concentrate in organisms and toxicity. The Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 is a relevant bioindicator of TE coastal contamination. However, little research has studied the combined influence of environmental condition changes and physiological processes on their kinetics in that species. Caged M. galloprovincialis were thus immerged in 2 contrasted pristine Corsican (France) coastal environments, the semi-enclosed Diane salty pond and the open Calvi Bay, from February to June 2011. Mussels were regularly sampled to study the kinetics of 19 TEs in their flesh; dissolved and particulate TEs were also monitored. The primary production and the water physico-chemical variables were measured, and meteorological data were purchased from Météo-France. TE kinetics in mussels differed between sites. Mussel spawning, a temperature and saline-induced physiological process that occurred about 10 days later in the Diane pond, was followed by a short time increase of TE levels in the mussel flesh. Mussel contamination also evolved according to changes of their respective environmental TE levels. Raining events temporary led, in the Diane pond, to the water enrichment with TEs, nutrients and detrital material, to peaks of primary production and to the increase of TE concentrations in the mussel flesh. This step by step evolution of TE levels in the environment and mussels was afterwards followed by a rapid return to initial conditions. In the open Calvi Bay, these fast and balanced kinetics were not so obvious, because of the rapid dilution of environmental constrain effects in the Bay. Mussels are often used as bioindicator in estuaries and coastal enclosed meadows with rapidly changing environmental conditions. In such conditions, the influence of the environment on TE kinetics in mussels must be considered, in addition to physiological processes, when monitoring the TE coastal contamination. [less ▲]

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See detailThe influence of bioavailable copper and zinc concentrations on metallothionein levels, DNA damage and gene expression in the polychaete Nereis (Alitta) virens (M. Sars, 1835)
Pini, Jennifer; Richir, Jonathan ULg; Watson, Gordon

Poster (2014, December)

Nereis (Alitta) virens is an ecologically and commercially important polychaete of intertidal soft sediment and an ideal species to investigate long term effects of metals. Using a spike approach, worms ... [more ▼]

Nereis (Alitta) virens is an ecologically and commercially important polychaete of intertidal soft sediment and an ideal species to investigate long term effects of metals. Using a spike approach, worms (1-3 g) were incubated for nine months in sediments spiked at environmentally relevant concentrations of copper, zinc and copper & zinc together: low (copper: 70 mg kg-1, zinc: 200 mg kg-1), medium (copper 120 mg kg-1, zinc: 270 mg kg-1) and high (copper 575 mg kg-1, zinc: 1160 mg kg-1) concentrations. These concentrations were based on an extensive sampling regime of sediment, pore water and worms from seven sites with different levels of contamination across the UK. Worms were fed and maintained under ambient conditions in a flow-through seawater system and sampled at 3, 6 and 9 months. Using BCR sequential extraction, bioavailable metal concentrations in the sediment were assessed in addition to pore water and tissues metal concentrations. The induction of metallothionein (MT) activity, especially at month 6, revealed the detoxification potential of N. virens under metal stress conditions. Significant correlations were obtained between copper bioavailable concentrations in the sediment and MT levels at month 3 and between zinc bioavailable concentrations in the sediment and MT levels at month 6. The highest DNA damage was recorded at month 3 for high copper & zinc combined treatment with 36.44%. Significant correlations were obtained between sediment bioavailable metal concentrations and DNA damage. In addition, the study of metal induced gene expression will reveal for the first time metal regulation process in the polychaete N. virens. This study showed that (1) not only high copper was toxic to N. virens but high copper & zinc combined treatment was the most toxic to the worms and (2) MT and DNA damage were sensitive and reliable endpoints used to evaluate copper and zinc toxicity. [less ▲]

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See detailPoster session 3: Thursday 4 December 2014, 14:00-18:00Location: Poster area.
Gabriels, C.; LANCELLOTTI, Patrizio ULg; Van De Bruaene, A. et al

Poster (2014, December)

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See detailPoster session 4: Friday 5 December 2014, 08:30-12:30Location: Poster area.
Uejima, T.; Itatani, K.; Nakatani, S. et al

Poster (2014, December)

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See detailWood digestion in lower termites: multidisciplinary approaches based on differential feeding
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, December)

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical ... [more ▼]

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical environments found in lower termites paunch are potentially the key point to explain the efficiency of ligno-cellulose digestion. Contribution of advancing technologies accelerates the progress of our knowledge in this field. Here, we present multiple approaches combining old and recent techniques used to highlight the effect of ligno-cellulosic compounds on termite gut and the role of populations from the symbiotic microbial community. Termites Reticulitermes flavipes (Kollar) submitted to various artificial diets showed variations in flagellates populations profile and enzymatic activities. Differential protein expression was investigated using 2D-DIGE MALDI-TOF-TOF and 2D-LC-MS/MS using high resolution orbitrap analyzer. Results from both proteomic experiments tend to support each-other and bring complementary points of view. The gel-free analysis resulted in highly contrasted identification of enzymes involved in ligno-cellulose digestion and metabolism. Finally, differential feeding experiments leaded to in vivo selection of different symbiotic communities. These communities were characterized following some metabolism assays and allowed the cultivation of diverse microbial consortia using media closely related to the respective artificial diets. This work provides relevant data on termite and associated microbial community response to alimentary diets. [less ▲]

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See detailEffect of tree species mixture on earthworm communities on a continental scale
De Wandeler, Hans; Baeten, Lander; Carnol, Monique ULg et al

Poster (2014, December)

The belowground food web represents a major part of associated biodiversity in forest ecosystems, and plays a significant role in the ecosystem processes of litter decomposition and nutrient turnover ... [more ▼]

The belowground food web represents a major part of associated biodiversity in forest ecosystems, and plays a significant role in the ecosystem processes of litter decomposition and nutrient turnover. Past research has demonstrated overwhelming evidence of strong tree species identity effects on earthworm communities. It has been proposed that increased plant community diversity would be beneficial to the abundance and diversity of the belowground food web, but effects of tree species diversity on earthworm communities have seldom been reported, and are inconclusive. In this study at continental scale we evaluated whether tree species diversity positively affects earthworm biomass and diversity. For this purpose the FunDivEUROPE Exploratory Platform was used with 209 plots in 6 regions well spread over Europe with a low within-region site variability, but a within-region tree species diversity gradient from monocultures to 3 or 4 species plots. In every plot earthworms were sampled using a combined method of mustard extraction and hand sorting of litter and a soil monolith. Data are being analysed with multivariate tools and mixed effects models. First results suggest only limited influence of tree diversity on the biomass of earthworm communities at continental scale. Tree diversity effects are weak, context specific and interacting with tree identity. In nutrient poor soils we found a negative tree diversity effect on earthworm biomass when deciduous monocultures are enriched with coniferous species, while in rich soils we found a positive tree diversity effect which could be related with the food security this provides to the earthworm community. [less ▲]

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See detailImpact of shading on meiofauna in a Posidonia oceanica meadow
Pete, Dorothée ULg; Gobert, Sylvie ULg

Poster (2014, December)

Posidonia oceanica meadow is an endemic ecosystem of the Mediterranean coasts. A known threat to this ecosystem is aquaculture. In zones of intensive fish production, P. oceanica meadow tends to be less ... [more ▼]

Posidonia oceanica meadow is an endemic ecosystem of the Mediterranean coasts. A known threat to this ecosystem is aquaculture. In zones of intensive fish production, P. oceanica meadow tends to be less healthy or to disappear .One of the reasons for this is a decrease in the light that reaches the leaves (direct shading, increase of water turbidity or of epiphytic algae density). Unfortunately, when the meadow begins to die, it is often too late to act. So, people are trying to find indicators that react early to this kind of perturbations. In this framework, this study focuses on the impact of shading (without nutrient enrichment) on the meiofauna living in the surface sediment of a P. oceanica meadow. An in situ shading experiment was led from the end of May to the end of August 2009, at a depth of 10 m, in a reference P. oceanica meadow. Three shading nets were put in the meadow to reach a light extinction of 50%. A control site was also defined. The first two centimetres of sampled sediment cores were studied. After three months of shading, the total abundance of meiofauna at the shading site was lower than at the beginning of the experiment, while it stayed around the same level at the control site. This difference is mainly due to a decrease in the total number of foraminiferans, nematods, gnathostomulids, copepods and bivalves. However, no significant difference in diversity was observed. At the end of this experiment, it appeared that, contrarily to what is mostly said in the literature, the direct organic enrichment that occurs at fish farms is not the only reason to the modification of the meiofauna communities of the ecosystem. The shading by itself has also an effect. [less ▲]

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See detailEvidence of a fine-scale genetic structure for the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees
Gillet, François ULg; Cabria Garrido, Maria Teresa; Blanc, Frédéric et al

Poster (2014, December)

The Pyrenean desman (Galemys pyrenaicus) is a small semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in mountain streams of cold and well ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is a small semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in mountain streams of cold and well-oxygenated flowing waters (Nores et al. 2007). This species is currently considered as vulnerable in the IUCN Red List (Fernandes et al. 2008) and has been suffering from habitat loss and fragmentation for decades, inevitably impacting its distribution. The ecology and biology of this species are poorly known, notably because of its elusive behavior and its primarily nocturnal activity (Stone 1987, Bertrand 1994). Its distribution area is even not definitively established. Furthermore, a recent genetic study, based on mitochondrial and intronic sequences (Igea et al. 2013), showed that the genetic variability of the Pyrenean desman is very low in the Pyrenees. In this study we investigated the potential existence of a genetic structure and gene flow at a smaller scale using 24 polymorphic microsatellites loci. As the Pyrenean desman is a very elusive species, we completed our sample collection of tissues with faeces samples coming from the French distribution area of this species. Doing so, we successfully identify 70 individuals out of 355 faeces samples. Bayesian analyses revealed a cryptic genetic structure in our data set. Three clusters were evidenced (one western, one central and one eastern) and gene flow appears to be limited between these clusters (min. Fst value of 0.2). [less ▲]

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See detailPoster session 5: Friday 5 December 2014, 14:00-18:00Location: Poster area.
Henri, C.; DULGHERU, Raluca Elena ULg; Magne, J. et al

Poster (2014, December)

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See detailClub 35 Poster session 2: Thursday 4 December 2014, 08:30-18:00Location: Poster area.
Voilliot, D.; Magne, Jm; DULGHERU, Raluca Elena ULg et al

Poster (2014, December)

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See detailPerceptions culturelles dans un contexte de néoplasme pédiatrique: Considérations transculturelles entre l’Occident et le Japon
Vander Haegen, Marie ULg; Roskam, Isabelle

Poster (2014, November 29)

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et ... [more ▼]

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et moraux sur base des repères culturels alloués depuis des décennies. Investiguer les balises culturelles et l’appréhension sociétale du néoplasme pédiatrique reste peu développé dans la littérature. Ce champ « anthropo-clinique » prend initialement son essor dans la clinique oncologique adulte où des résultats significatifs montrent un lien entre la culture et la perception du cancer. OBJECTIFS Les objectifs de cette étude sont multiples: (a) étudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant, (b) analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale, et (c) mettre en exergue les diverses implications cliniques pour les professionnels de la santé. METHODE Une revue de littérature est conduite afin de dégager les tendances culturelles dans la politique sanitaire, le service hospitalier, la relation médecin-malade, le concept de douleur, l’environnement familial et la perception de la mort au Japon et en Occident. Le choix s’est porté sur ce pays asiatique par la présence de certains repères culturels intéressants dans le domaine de l’oncologie pédiatrique. RESULTATS (a) Etudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant Les correspondances effectuées ont permis d’entrevoir la situation onco-pédiatrique comme relevant de faits biologiques et de faits culturels. Les paramètres culturels interviennent au sein des nombreuses conceptions et représentations sociales de la maladie cancéreuse d’un enfant. L’ensemble de ces perceptions sociales étant notamment influencées par les valeurs et les truismes véhiculés dans la culture. (b) Analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale. Eléments de similitudes : Il a été noté l’universalité des mécanismes pathogéniques du cancer chez l’enfant et le développement de politiques sanitaires dans la lutte contre le cancer. Eléments de dissimilitudes : A. Le Japon Le système familial japonais et son organisation des soins en cancérologie de l’enfant varient et se teintent de nombreuses coutumes shintoïstes et bouddhistes. Il a été identifié le chaos régnant dans ses familles où des attitudes désengagées existent. L’organisation hospitalière japonaise est décrite comme carencée en réseaux de soutien psychologique pour la mère, très souvent seule face à la maladie de son enfant. Des risques majeurs de dépression, de traits anxieux et d’un stress post-traumatique sont observés. Le médecin japonais dispose d’un pouvoir de décision médicale et sociale, se manifestant notamment par un degré de variabilité dans la communication diagnostique et pronostique envers la famille et l’école d’origine. La situation de soin est variable avec l’inclusion en premier lieu d’une pharmacopée naturelle afin de traiter le cancer et ses effets iatrogènes. Enfin, il subsiste une inhibition du partage social des émotions où, des attitudes de retenue et de silence chez les parents se manifestent durant tout le processus thérapeutique mais aussi en cas de décès de l’enfant. B. L’Occident Les pays occidentaux présentent une appréhension sociale et médicale singulière du cancer pédiatrique. La variabilité observée concerne l’organisation hospitalière et la communication médicale. Le praticien occidental établit une communication médicale ouverte avec l’enfant et sa famille et ce, dès le départ du processus thérapeutique. Le contexte curatif est caractérisé par une forte médication d’opioïdes afin de pallier aux nombreuses douleurs et effets des traitements. L’aménagement hospitalier permet la création de réseaux de soutien psychologique pour l’enfant et sa famille et l’établissement d’activités ludiques.(c) Mettre en exergue les diverses implications cliniques pour les professionnels de la santé L’intention de cette étude est de sensibiliser les professionnels de la santé à la pléiade de facteurs psycho-sociaux intervenant dans un contexte de maladie néoplasique. Éléments qui doivent requérir d’une bienveillance clinique où tant l’enfant traité que son entourage familial, doivent être entendus dans toute leur spécificité d’être humain. DISCUSSION Une stratégie d’action intéressante concernerait une grille de lecture culturelle du patient et de sa famille. Cette grille peut offrir un entendement de la diversité culturelle en psycho-oncologie et peut permettre au professionnel de réajuster finement son travail clinique dans un contexte quotidien de multiculturalité. Cette grille de lecture doit rester simple et proposer un petit nombre de « repères culturels » (items culturels) pour professionnels, leurs permettant d’ajuster certains paramètres cliniques (communication médicale, gestion des émotions, rapport à la mort, etc.). Cette considération ethnologique du patient doit supposer au préalable une formation de compétences dans le domaine de la culture et de la santé/maladie. [less ▲]

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See detailEstimating Ventricular Stroke Work from Aortic Pressure Waveform
Kamoi, Shun; Pretty, Christopher; Chiew, Yeong Shiong et al

Poster (2014, November 28)

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See detailTracking stressed blood volume during vascular filling experiments
Pironet, Antoine ULg; Dauby, Pierre ULg; Chase, J. Geoffrey et al

Poster (2014, November 28)

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac ... [more ▼]

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac output after fluid infusion. [less ▲]

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See detailFunctional analysis of FRD3 in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Motte, Patrick ULg et al

Poster (2014, November 28)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

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See detailRole of the polyQ length and non-polyQ regions during the aggregation process into amyloid fibrils of model polyQ proteins
Huynen, Céline ULg; Willet, Nicolas ULg; Buell, Alexander K et al

Poster (2014, November 26)

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated ... [more ▼]

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated proteins. This polyQ expansion is thought to be the major determinant in the development of neurotoxicity, triggering protein aggregation into amyloid fibrils. A large body of evidence however suggests that non-polyQ regions modulate the aggregation process triggered by polyQ expansions. The interplay between the polyQ tract and non-polyQ regions is complex and still not fully understood. In order to better understand it, we previously designed and characterized model polyQ proteins made of the beta-lactamase BlaP with 23, 30, 55 and 79Q inserted at position 197 or 216. Our first results had indicated that our model is relevant to study polyQ aggregation since it recapitulates the aggregation properties of polyQ disease-associated proteins: there is a Q-threshold for the spontaneous formation of amyloid fibrils in solution, and above the threshold, the longer the polyQ, the faster the aggregation. Moreover, the structure of BlaP and the position of insertion of the polyQ tract influence their aggregation properties in solution. This work aims to better understand, at the molecular level, (i) the precise role of the polyQ length (23, 30, 55, 61, 67, 73 and 79Q), (ii) the conformation of the host protein (native or unfolded BlaP), (iii) the location of the polyQ tract within BlaP (197 or 216), (iv) the flexibility of the polyQ flanking sequences, and (v) the origin of constraints applied by BlaP to the inserted polyQ tract (at its N- or C-terminal end) on the structural, thermodynamic and aggregation properties of BlaP-polyQ chimeras, using a wide range of biophysical techniques (e.g., spectroscopy methods, quartz crystal microbalance, atomic force microscopy and dynamic light scattering). The effect on the aggregation properties will be determined on the spontaneous aggregation into amyloid fibrils in solution, and on the nucleation and on the elongation steps of amyloid fibril formation. For this purpose, new chimeras containing 61, 67 and 73Q at position 197, or 55Q inserted at position 197 in between two different protease’s cleavage sites, that are relatively flexible, will be moreover created. Our results first demonstrate that the spontaneous aggregation into amyloid fibrils in solution is correlated to the polyQ length with an exponential growth function, and that the elongation rate is linearly correlated to the polyQ length, independently of the protein context (i.e., conformation of BlaP, and/or location of the polyQ tract, and/or polyQ peptides inserted or not within BlaP). However, the location of the polyQ tract inside BlaP, and/or its conformational state, and/or the flexibility of polyQ flanking sequences, and/or the origin of constraints applied to the polyQ tract drastically influence the ability of a polyQ tract to trigger the nucleation and/or the elongation step of amyloid fibrils (variation in the Q-threshold and in the absolute rate of both steps). Altogether, our results suggest that non-polyQ regions constitute an additional potential therapeutic target, more specific than drugs targeting the polyQ sequence, to interfere with the nucleation and/or the elongation of amyloid fibrils, associated to neurotoxicity. A possible drug could be constituted by a ligand specific to non-polyQ regions of disease-associated proteins, which further increases the constraints applied to the polyQ expansion to prevent the disease onset and/or progression. [less ▲]

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See detailUse of 13C-NMR in structural elucidation of polysaccharides: case of locust bean gum
Gillet, Sébastien ULg; Aguedo, Mario ULg; Blecker, Christophe ULg et al

Poster (2014, November 25)

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic ... [more ▼]

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic structure is the same for all galactomannans (Fig. 2). However, when locust bean gum is extracted at different temperatures, the generated fractions exhibit different properties in aqueous solution (viscosity, viscoelasticity, gel formation, thermohydrolysis resistance, etc.). This means that there are differences within the fine structure of the polymers (although the basic structure is the same). Analysis of [13C]-NMR spectra of galactomannans, in combination with other techniques, can provide capital information about fine structural elucidation of the polymers. The method specifies the distribution of lateral galactosyls along the main chain of mannans. Two fractions extracted from locust bean gum at 25 and 80 °C (respectively GM25 and GM80) were comparatively studied by [13C]-NMR. Mannosyls/Galactosyls (M/G) ratios can be determined by considering the intensities of C-1 mannose and galactose signals in [13C]-NMR spectra. This method provides results relatively close to those obtained by GC-MS analysis. Spectra also showed that resonance from C4 of D-mannose residues were split, in evident dependence upon the nearest-neighbor probabilities (“diad frequencies”) of D-galactosyl groups along the mannan chains (Fig. 2). Diad frequencies were obtained by integrating C4(Man) peak areas. F11, F21/F12 and F22 gave respectively the di-, mono- or non-substituted mannose pairs proportions. High percentages of F11 and F22 therefore indicate a more non-homogeneous distribution of lateral galactosyls along the polysaccharide backbone as observed for GM80. The percentages of total lateral substituents obtained by C4(Man) peak analysis [F11 + (F21 or F12)/2] were fairly well correlated with M/G ratios. Splitting of the C-6 substituted D-mannose resonance provides, therefore the basis for determining the next-nearest-neighbor probabilities (triad frequencies) (Fig. 2). However, the spectrum is often not sufficiently resolved to accurately quantify and interpret the results. [less ▲]

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See detailImpact of steam explosion treatment on chemical configuration of Tall Fescue lignin : structural elucidation using NMR spectroscopy
Maniet, Guillaume ULg; Jacquet, Nicolas ULg; Gillet, Sébastien ULg et al

Poster (2014, November 24)

In the economic and energy context of our society, it is universally recognized that alternatives to petrochemicals products must be found. To overcome this problem, renewable lignocellulosic biomass ... [more ▼]

In the economic and energy context of our society, it is universally recognized that alternatives to petrochemicals products must be found. To overcome this problem, renewable lignocellulosic biomass could be used to produce high value products. To achieve this objective, pretreatment processes are required to allow the breakdown of lignocellulosic structure and increase accessibility of the material. In this way, steam explosion is a thermo-mechano-chemical pretreatment which allows the opening of lignocellulosic material structural components and includes modifications of the physical properties of the material, hydrolysis of hemicellulosic components and modification of the chemical structure of lignin [1]. This study is focused on the impact of various steam explosion treatments on the chemical configuration of tall fescue lignin. NMR analyses perform on the Festuca L. pretreated samples show variations of links with treatment intensity. Observations show double phenomen :re-polymerization and depolymerization of the lignin structure during steam explosion process [2]. In parallel, HPSEC analyses show modifications in the molecular weight of the lignin obtained after the steam explosion treatment. [less ▲]

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See detailGNSS observational bias in the frame of ionospheric studies
Wautelet, Gilles ULg; Warnant, René ULg

Poster (2014, November 17)

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See detailSpecific properties of bone marrow mesenchymal and neural crest-derived stem cells: Relevance in spinal cord injury therapy.
Neirinckx, Virginie ULg; Agirman, Gulistan ULg; Marquet, Alice ULg et al

Poster (2014, November 17)

Spinal cord injury (SCI) treatment represents a critical issue in clinical research and patient care. Stem cell-based replacement therapies have already been proposed worldwide, especially studying stem ... [more ▼]

Spinal cord injury (SCI) treatment represents a critical issue in clinical research and patient care. Stem cell-based replacement therapies have already been proposed worldwide, especially studying stem cells from the adult bone marrow stroma. Previous studies focusing on those cells did not specifically consider their intrinsic embryonic heterogeneity, thus intermingling different stem cells subpopulations to treat experimental SCI or even injured patients. In this study, we decided to compare adult bone marrow neural crest-derived stem cells (NCSC) and mesenchymal stem cells (MSC), and highlight which of their specific properties could be relevant in therapeutic perspectives. In that purpose, we compared NCSC and MSC isolated from adult mouse bone marrow. We then compared the effects that both cell types could exert once grafted inside an injured spinal cord. Cells were injected into the spinal cord of mice that right after a spinal cord contusion at the T11-12 spinal level. Our results indicate that both MSC and NCSC-injected mice recovered locomotion abilities faster than control mice (as assessed by BMS scoring). Additionally, we observed that after 28 days post-injury, the lesion volume tended to decrease in mice that received cell graft compared to control group. Interestingly, it appeared that MSC seemed to be able to modulate inflammation inside the lesion, more than NCSC. Indeed, MSC-graft increased early neutrophil and macrophage recruitment in the bloodstream and inside the spinal cord, and increased the number of arginase-1-expressing cells remaining in the spinal cord after 28 days. In parallel, we compared the secretome of both NCSC and MSC, and noticed some interesting differences: MSC secreted several chemokines reflecting possible immunomodulating properties, while NCSC secreted products might be able to enhance neurite outgrowth. Indeed, preliminary data showed that NCSC induced neuritogenesis on primary neurons in vitro. Altogether, those results should help to improve and optimize cell-based therapies parameters and/or to define precise and efficient pharmacological treatments for SCI patients. [less ▲]

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See detailGlutamate controls brain estrogen synthesis during sexual interactions
de Bournonville, Catherine ULg; Aourz, Najat; Van Eeckhaut, Ann et al

Poster (2014, November 17)

Besides their long-lasting effects mediated by a modulation of gene transcription, brain-derived estrogens can rapidly regulate (within minutes) reproductive behaviors. In vitro, the activity of aromatase ... [more ▼]

Besides their long-lasting effects mediated by a modulation of gene transcription, brain-derived estrogens can rapidly regulate (within minutes) reproductive behaviors. In vitro, the activity of aromatase (AA), the enzyme responsible for the conversion of androgens into estrogens, is also regulated on a similar short time-scale, via phosphorylation of the enzyme resulting from changes in neuronal activity or glutamate release. Acute changes in AA have been documented ex vivo in specific brain regions following exposure to social or stressful stimuli but the mechanism underlying these regulations is not known. To investigate whether glutamate is implicated in these rapid changes in AA, male quail received a unilateral injection of kainate in the medial preoptic nucleus (POM). The left and right preoptic areas were collected 20 min later and assayed separately by the tritiated water technique for AA. As shown previously in preoptic explants maintained in vitro, AA was downregulated in the kainate-injected hemisphere as compared to the non-injected side. To determine whether the decline in AA detected in the POM after a sexual interaction could be mediated by an increased release of glutamate in this region, extracellular glutamate concentration was measured by in vivo microdialysis with a probe implanted in the POM of sexually mature males. Dialysate was collected every 3 minutes over three periods of 15 min when the male was (1) alone, (2) allowed to freely copulate with a female and (3) alone again. A transient rise in extracellular glutamate concentration was observed specifically and immediately after the expression of cloacal contact movements, when semen is transferred to the female. Glutamate returned to a basal level after the female was removed. Together, these results indicate that the mechanism of acute regulation of aromatase activity by glutamate identified in vitro is potentially responsible for the acute regulation of the enzyme observed in vivo following copulation. As rapid changes in brain estrogen synthesis and its actions are apparently related to the control of sexual motivation rather than sexual performance, follow up experiments should now determine whether the release of glutamate in the POM occurs in parallel with an increase in motivation or follows the termination of the copulatory sequence. [less ▲]

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See detailContrasting the role of Ih and ICaT currents in post-inhibitory rebound mechanisms in reciprocal-inhibitory networks
Dethier, Julie ULg; Drion, Guillaume ULg; Sepulchre, Rodolphe

Poster (2014, November 16)

Models with reciprocal inhibition are ubiquitous in the literature. For instance, common rhythmic motor behaviors produced by central pattern generators (CPGs) involve half-center oscillators, which ... [more ▼]

Models with reciprocal inhibition are ubiquitous in the literature. For instance, common rhythmic motor behaviors produced by central pattern generators (CPGs) involve half-center oscillators, which consist of two inhibitory neurons that are not endogenous oscillators, but produce rhythmic outputs when reciprocally connected (Marder & Calabrese 1996). Models of thalamocortical spindle oscillations also suggest that the rhythm originates from the thalamic reticular nucleus, which consists in interacting inhibitory nonoscillatory neurons (Wang & Rinzel 1992). [less ▲]

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See detailEffect of “diagnosis threat” in clinical setting
Fresson, Megan ULg; Dardenne, Benoît ULg; Meulemans, Thierry ULg

Poster (2014, November 14)

Objective. When reminded of their neurological history, mild traumatic brain injured (TBI) students underperform on neuropsychological tests (Suhr & Gunstad, 2002). To date, this “diagnosis threat” (DT ... [more ▼]

Objective. When reminded of their neurological history, mild traumatic brain injured (TBI) students underperform on neuropsychological tests (Suhr & Gunstad, 2002). To date, this “diagnosis threat” (DT) phenomenon has mainly been studied with a non-clinical and high-functioning population (university students). The aim of this study was twofold: to study this phenomenon with neurological patients and to examine the mechanisms responsible for underperformance. Method. Patients (18-55 years-old) who had sustained a TBI or a stroke were recruited from ambulatory and hospitalized cares, and then assigned to one of three conditions : Patients attention was drawn on (1) their neurological disease and the neuropsychological components of the upcoming tasks (DT group) ; (2) their intact sensory capacities and the sensorial components of the tasks (Neutral group); or (3) their better cognitive abilities compared to Alzheimer disease patients (Stereotype boost group). After these instructions, patients carried out cognitive tasks and completed questionnaires. Results. Preliminary analyses (n=18) showed that, on the z-score of executive functioning, the DT group performed worse than both the neutral group (p=.03) and the stereotype boost group (p=.05), but did not differ for the attentional and memory scores. Instructions also had an impact on cognitive self-efficacy, with the neutral group demonstrating greater score than the negative one (p=.08). Furthermore, the self-efficacy score tended to correlate with the score of executive functioning (r=.37). Conclusions. Results show that the DT phenomenon has an impact on cognitive performances in clinical setting, at least on executive functions, which are usually demonstrated to be the most sensitive to stereotype effects. [less ▲]

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See detailEconomic efficiency of milk production farms in Hanoi Suburbs, Vietnam
Le Dinh, Khan; Phan Dang, Thang ULg; Lebailly, Philippe ULg et al

Poster (2014, November 14)

Dairy production has presented in Vietnam since 50 years, but these dairy farms really developed from 2001. In the period 2001 – 2010, the average growth rate of dairy herds was up to 30% per year ... [more ▼]

Dairy production has presented in Vietnam since 50 years, but these dairy farms really developed from 2001. In the period 2001 – 2010, the average growth rate of dairy herds was up to 30% per year. However, fresh milk production satisfies about 20-25% of milk consumption. The rural areas of Hanoi Province are large for dairy production. This province builds the 7 agro-ecological sub-regions for the development of dairy farming, in which Ba Vi District is a most of sub-regions for dairy production development. But the average size is about 2.5 cows per farm. This research aims to better understand how the factors in milk sector adapt to current economic conditions in Hanoi suburbs through analyzing farming systems. These studies were conducted from January to August 2012 on 43 dairy farms with different production scales at Ba Vi District to characteristic of dairy farming systems. The data were collected by interviewing and keeping records at the farms that have milk production. The results showed that three dairy production systems in these zones: (1) Dairy small farming system has from 1 to 3 cows per farm; (2) The medium farming system has from 4 to 5 cows per farm; and (3) The dairy intensive farming system has more 5 cows per farm. The economic activities were really diversified with landless per household farm such as rice production, gardens, grass production and sylviculture. Land resources per farm of intensive farming system were largest including 9,239 m² for garden around the household; 2,574 m² for rice land; 563 m² of grass area and 3,325 m² for sylviculture. While the small farming system has the lowest potential land, each household has only 2,815 m² of garden; 1,795 m² of rice land; 2,095 m² of grazing land. The medium farming system has about 3,150 m² of garden; 2,220 m² of rice land and 3,891 m² of grazing land. Milk production cycle was quite long with intensive farming system, up to 324 days comparing with 299 days per cycle with medium farming system and to 306 days per cycle with small-scale farmers. This difference was not statistically signification (P> 0.05). Average milk production yield per cow was high with medium farming system (16 kg per cow per day) in comparison with 15 kg per day at intensive and small farming systems (P> 0.05). Milk production yield has been improved in the past years with crossbreed cows such as Holstein-Friesian 50% and 75%. But this productivity was still considered to be lower than the milk production yield with dairy cows raised in Moc Chau District under Son La Province with 20.5 kg per cow per day and 6,250 kg of milk per cow per cycle). In addition, in the period from 2006 to 2012, fresh milk prices at farm gate have continued to rise for the farmers, while feed prices increased in the period from 2006 to 2010 and these feed prices were stable between 2011 and 2012. In the period of 2006 to 2012, fresh milk prices at farm gate increased from 0.17 USD per kg in 2006 to 0.60 USD per kg in 2012 and feed prices were from 0.15 USD per kg in 2006 increasing to 0.39 USD per kg in 2012. The net incomes were calculated per a cow per year reached 1,274 USD with the small-scale farming households to 1,476 USD with medium farming system. The net income per kg of fresh milk at farm gate obtained from 0.28 USD with small farming system to 0.31 USD with medium farming system. The net household income was high from dairy production, from 2,437 USD per farm per year with small farming system to 5,487 USD per farm per year with intensive farming system. The net labor family income gained from 997 USD per active per year with small farming system to 2,757 USD per active per year with intensive farming system. Thus, dairy production farms in recent years in Hanoi Suburbs provide very high economic efficiency for farmers in comparison with other agricultural activities at the farms. This explains why the rate of dairy family farming has grown very fast in the studied zones in the period from 2009 to 2012. This trend will continue to grow in the future. This research was also showed that the price of fresh milk plays a crucial role for profitable at farm level. But medium farming system with the size from 4 to 5 dairy cows per farm is more profitable with current economic conditions at farms. [less ▲]

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See detailIs there a global procedural learning deficit in children with Specific Language Impairment ?
Desmottes, Lise ULg; Maillart, Christelle ULg; Lejoly, Kelly ULg et al

Poster (2014, November 13)

The study of procedural learning abilities in children with Specific Language Impairment (Procedural Deficit Hypothesis, PDH; Ullman & Pierpont, 2005) remains a relatively unexplored field of research ... [more ▼]

The study of procedural learning abilities in children with Specific Language Impairment (Procedural Deficit Hypothesis, PDH; Ullman & Pierpont, 2005) remains a relatively unexplored field of research. Since most evidence comes from studies using tasks which involve learning of sequenced patterns, research using other procedural learning paradigms (like motor adaptation tasks) is needed to further evaluate the PDH in children with Specific Language Impairment (SLI). Therefore, in this study, we examined the ability of children with and without SLI to learn, consolidate and generalize a mirror-tracing task, a paradigm that does not involve sequence learning and had never been used in SLI. Children with SLI and typical developing (TD) matched children participated in the study. Children with SLI were included if they scored below -1.25 SD of the expected normative performance in at least 2 language areas. Both groups had to trace ten 5-pointed stars seen only in mirror-reversed view in two learning sessions separated by a one-week delay. The transfer phase consisted in tracing a new figure. The time required to complete the tracing, and the number of errors committed were recorded. Full results will be presented and discussed during the presentation of the paper. [less ▲]

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See detailWhen food cultures meet. Ethnographic analysis of belongings and positionings.
Mescoli, Elsa ULg

Poster (2014, November 13)

The displacement of human beings is always accompanied by the movement of things and practices which travel with them. This is an obvious statement that indeed leads to interesting studies on the material ... [more ▼]

The displacement of human beings is always accompanied by the movement of things and practices which travel with them. This is an obvious statement that indeed leads to interesting studies on the material culture involved by people’s mobility. Within this culture are food habits, objects of numerous scholars’ researches often focusing on culinary changes (or resistances) linked to migration paths. Far for being the only issue at stake when studying migrant’s food practices – moreover frequently assumed as originally static prior to migration –, this matter is likely to overshadow other significant dynamics. Such as those which occur when supposedly different food cultures meet, thus leading to the mobilization of belongings by groups who want to take a specific position toward one another. My paper is aimed at analysing this encounter. The assumption is that it shapes a space which corresponds to a tertium quid, that is a dimension where not only the boundaries of food cultures fade, proving of an original indeterminacy and interchangeability of practices, but also where individuals constantly change their reciprocal position depending on the perspective of the gaze upon them. To show these dynamics, I will present the ethnographic data stemmed from my doctoral research about the culinary practices of a group of Moroccan migrant women living in Milan hinterland. I will support my presentation with visual materials collected during an eighteen month fieldwork which included observations and interviews inside homes as well as in public events such as an Arab cooking class. [less ▲]

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See detailInfluence de la vitesse de croissance sur le classement de lames de bardage de Douglas wallon (Belgique)
Pollet, Caroline; Henin, Jean-Marc; Hebert, Jacques ULg et al

Poster (2014, November 12)

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See detailDevelopment of a high throughput de novo sequencing platform for peptidic toxins combining proteomics and transcriptomics
Degueldre, Michel ULg; Verdenaud, Marion; Zuniga, Sheila et al

Poster (2014, November 07)

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See detailUse of lipid probes as matrices for MALDI Imaging applications
Longuespée, Rémi ULg; Alberts, Deborah ULg; Pottier, Charles et al

Poster (2014, November 07)

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See detailTh17 cells in melanoma microenvironment
Weatherspoon, Alodie ULg; Multon, Sylvie ULg; Defaweux, Valérie ULg et al

Poster (2014, November)

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See detailIon mobility-mass spectrometry to perform structural classifications of disulfide-bridged-peptides
Massonnet, Philippe ULg; Upert, Gregory; Morsa, Denis ULg et al

Poster (2014, November)

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See detailTreg/Th17 balance during murine embryo implantation and pregnancy
Polese, Barbara ULg; Gridelet, Virginie ULg; Araklioti, Eleni et al

Poster (2014, November)

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See detailSystematic Analysis of two cystatin C assays using samples of 2057 older adults - The Berlin initiative study
Ebert, natalie; DELANAYE, Pierre ULg; Martus, Peter et al

Poster (2014, November)

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See detailMicrowave-assisted extraction of pectin from unused pumpkin biomass
Košťálová, Zuzana; Aguedo, Mario ULg; Hromádková, Zdenka

Poster (2014, November)

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See detailDistribution and robustness of a distance-based multivariate coefficient of variation
Aerts, Stéphanie ULg

Poster (2014, November)

When one wants to compare the homogeneity of a characteristic in several popula- tions that have di erent means, the advocated statistic is the univariate coe cient of variation. However, in the ... [more ▼]

When one wants to compare the homogeneity of a characteristic in several popula- tions that have di erent means, the advocated statistic is the univariate coe cient of variation. However, in the multivariate setting, comparing marginal coe cients may be inconclusive. Therefore, several extensions that summarize multivariate relative dispersion in one single in- dex have been proposed in the literature (see Albert & Zhang, 2010, for a review). In this poster, focus is on a particular extension, due to Voinov & Nikulin (1996), based on the Mahalanobis distance between the mean and the origin of the design space. Some arguments are outlined for justifying this choice. Then, properties of its sample version under elliptical symmetry are discussed. Under normality, this estimator is shown to be biased at nite samples. In order to overcome this drawback, two bias corrections are proposed. Moreover, the empirical estimator also su ers from a lack of robustness, which is illustrated by means of in uence functions. A robust counterpart based on the Minimum Covariance Determinant estimator is advocated. [less ▲]

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See detailAnalysing spatiotemporal changes in sediment contamination
Richir, Jonathan ULg; Pini, Jennifer; White, Shannon et al

Poster (2014, November)

Coastal environments are subjected to anthropogenic threats, of which pollution by trace elements (TEs). They remain chemicals of concern because of their toxicity, their ability to be concentrated in ... [more ▼]

Coastal environments are subjected to anthropogenic threats, of which pollution by trace elements (TEs). They remain chemicals of concern because of their toxicity, their ability to be concentrated in biota and their persistence in sediment. Aware of these threats, monitoring agencies perform large environmental surveys. However, the databases generated often remain underexploited, even though they represent an important source of information for scientists. As a case study, we focused on the highly industrialized and developed coastal area of the Solent, south coast of the UK, which is also a European Marine site with protected habitats and species. To date, no scientific-based spatiotemporal trend has been published regarding its pollution in TEs. But mining of existing databases requested from the Environmental Agency and the Marine Environment Monitoring and Assessment National database showed that sediment from 32 sites were/are indeed monitored for that purpose, covering a 22 years period (1992-2013). Temporal trend analysis showed that sediment contamination significantly decreased during that time interval (45 % decrease in median). However, all TEs still show concentrations above Sediment Quality Guidelines. Important spatial variability is also present, presumably linked to the distribution of pollutant anthropogenic sources. Taken together, these data have been used to provide stakeholders with relevant scientific based tools, i.e. GIS maps of the contamination in TEs in the Solent. This regional-level assessment enables local stakeholders to create bridges between environmental scientists and local authorities and valorizes the existing databases, for future governance at the regional and national scales. [less ▲]

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See detailTMS can selectively activate and condition two different sets of excitatory synaptic inputs to corticospinal neurons in humans
Sommer, Martin; D'Ostilio, Kevin ULg; Cioccia, Matteo et al

Poster (2014, November)

Background: Current protocols or repetitive transcranial magnetic stimulation (rTMS) induce mixed facilitatory and inhibitory effects. More selective, quasi-monophasic high-frequency stimulators now ... [more ▼]

Background: Current protocols or repetitive transcranial magnetic stimulation (rTMS) induce mixed facilitatory and inhibitory effects. More selective, quasi-monophasic high-frequency stimulators now become available. We sought to investigate the impact of current direction and pulse width on intermittent theta burst stimulation (iTBS) effects on human motor cortex excitability. Also, we estimated strength-duration time constants from motor threshold and input-output (IO) curves for PA and AP orientations. Methods: We stimulated the dominant hand representation of the motor cortex in 15 healthy subjects, using “unidirectional biphasic” pulses generated by a controllable TMS machine (cTMS-3, Rogue Resolutions Ltd., Cardiff, UK), connected to a standard figure-8 coil. iTBS was applied conventionally, using 20 sequences of 2 seconds iTBS (10 bursts at 5 Hz burst repetition frequency, each burst consisting of 3 pulses of 80 % AMT intensity repeated at 50 Hz frequency). In separate sessions pulses differing in current direction and shape were applied: a) posterio-anterior (PA) current direction in the brain, 75 μs (iTBS_PA75). b) AP current direction, 45 μs (iTBS_AP45). Before and for 30 minutes after iTBS, we monitored the modulation of motor evoked potential (MEP) amplitude from the dominant first dorsal interosseus using conventional, monophasic, suprathreshold pulses generated by a Magstim 2002 stimulator, inducing PA currents in the brain, at 0.2 Hz frequency. In an additional study on ten healthy subjects, we investigated the effect the two coil orientations with three different pulse widths (30, 60 and 120 μs) on the IO curve and the latency of the motor evoked potentials (MEPs). Results: iTBS_AP45 yielded a pronounced and slightly delayed inhibition of MEP amplitude in all but one subjects, it was unrelated to the MEP latency differences. iTBS_PA75 had a variable and inconsistent effect that was in part related to the latency differenceAP-LM , in that long latency differences were correlated with the induction of inhibition rather than facilitation. We found a longer time constant for AP than PA orientation. MEP latencies yielded an interaction between pulse width and orientation, due mainly to longer onset latencies following AP stimuli of short duration. Conclusions: Current direction influences the outcome of iTBS, with a preference for AP currents. PA and AP stimuli activate the axons of neurones with different time constants. Those activated by AP pulses excite corticospinal outputs with a longer latency than those activated by PA pulses. AP pulses of short duration recruit long latency inputs most selectively. [less ▲]

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See detailHighly sophisticated compound droplets on fiber arrays
Weyer, Floriane ULg; Lismont, Marjorie ULg; Dreesen, Laurent ULg et al

Poster (2014, November)

The poster shows a drop of oil containing four soapy water droplets of four different colors : red, orange, green and blue. This sophisticated object is created thanks to a fiber array. Fiber networks ... [more ▼]

The poster shows a drop of oil containing four soapy water droplets of four different colors : red, orange, green and blue. This sophisticated object is created thanks to a fiber array. Fiber networks allow processes such as droplet motion, fragmentation, trapping, releasing, mixing and encapsulation. Therefore, by using a vertical fiber decorated with successive nodes, compound droplets can be created. A soapy water droplet, with a specific color, is placed at each intersection. Then, a silicon oil droplet glides along the vertical fiber, encapsulates and collects the water droplets at each node leading to the formation of a compound droplet with four different components. [less ▲]

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See detailImpact of the dialysis membrane on the Vitamin D metabolims markers
CAVALIER, Etienne ULg; DUBOIS, Bernard ULg; Urena, Pablo et al

Poster (2014, November)

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See detailRecyclage des briques cassées pour améliorer les propriétés céramiques des argiles plastiques (Meknès, Maroc)
El Ouahabi, Meriam ULg; Daoudi, Lahcen; El Boudour El Idrissi, Hicham ULg et al

Poster (2014, October 27)

Dans le Bassin de Saïs (nord ouest du Maroc), les argiles du Miocène de la région de Meknès sont utilisées dans l’industrie céramique locale pour la fabrication des briques. Ces argiles riches en smectite ... [more ▼]

Dans le Bassin de Saïs (nord ouest du Maroc), les argiles du Miocène de la région de Meknès sont utilisées dans l’industrie céramique locale pour la fabrication des briques. Ces argiles riches en smectite et en carbonates, ont une granulométrie très fine, marquée par la quasi absence de la fraction sableuse. Ces propriétés les rendent très plastiques, ce qui engendre des taux de retrait importants au séchage et à la cuisson et des problèmes de qualité du produit fini qui s’en suivent (fissurations, déformations et casses pendant les processus de séchage et de cuisson). Dans le but de palier à ce problème et d’améliorer la qualité du produit fini, nous proposons au cours de cette étude de recycler les déchets de briques cassées. Afin d'atteindre ces objectifs, 4 formulations ont été réalisées à base d’argiles plastiques, riches en smectite et des casses de briques broyées. Des quantités de 5, 10, 15 et 20% de briques broyées ont été ajoutées à la pâte destinée à la confection des briques. Des analyses minéralogiques par diffraction des rayons X (DRX), chimiques par spectrométrie de fluorescence (XRF), granulométrique par granulométrie laser, ainsi que les limites d’Atterberg ont été effectuées. Les résultats minéralogiques montrent une diminution de la teneur en carbonates (calcite) et en argile totale (principalement smectite), lors des ajouts successifs de briques broyées. La fraction sableuse augmente progressivement, jusqu’à atteindre 30% pour une formulation avec 20% de casses de briques. Cette granulométrie plus grossière diminue la plasticité de la pâte et permet d’éviter les déformations et les fissures pendant le processus du séchage. De plus, l’ajout des casses de briques dilue les carbonates, ce qui permet d’améliorer la qualité du produit cuit. Ces résultats indiquent que le recyclage des déchets de briques, présente un intérêt à la fois économique et écologique. Des essais de comportement au séchage et à la cuisson ainsi que des essais mécaniques complèteront cette étude. [less ▲]

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See detailReformage catalytique du toluène
Claude, Vincent ULg; Lambert, Stéphanie ULg; Courson, Claire

Poster (2014, October 23)

This poster summarizes results obtained for the catalytic reforming of tar in the oultlet gas of a biomass gasifier. All samples were synthesized with aluminum nitrate and an organosilane (EDAS) in order ... [more ▼]

This poster summarizes results obtained for the catalytic reforming of tar in the oultlet gas of a biomass gasifier. All samples were synthesized with aluminum nitrate and an organosilane (EDAS) in order to enhance the metallic dispersion and surface area. Three different compositions have been studied (Al2O3-SiO2; Al2O3-SiO2+2%wt Ni; Al2O3-SiO2+10%wt Fe). The catalytic tests highlight the fact that the sample containing 2%Ni wt is the more appropriate in our conditions. [less ▲]

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See detailAnalysis of the interactions of natural elicitor rhamnolipids with plant plasma membranes by in silico methods
Polo Lozano, Damien ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2014, October 20)

Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various ... [more ▼]

Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various chain lengths linked through a glycosidic bond to one or two rhamnose moieties. The fatty acids are linked together through an ester bond. These molecules have shown several biological activities including plant defense stimulation. It has be suggested that this elicitor activity could be related to an interaction of rhamnolipids with the lipid bilayer of the plant plasma membrane (PPM) and lead to its destabilization, which can activate the plant defense signaling pathways. In this context, interactions of two rhamnolipids (Rha-C10-C10 and Rha-Rha-C10-C10) with membrane models and lipidic constituents of the PPM were investigated using in silico approaches. Most probable chemical structures of the rhamnolipids were determined using the STRUCTURE TREE procedure according to the molecule potential energy. The ability of these rhamnolipid structures to insert within the PPM was assessed using IMPALA simulations. IMPALA uses a membrane model in which phospholipids molecules are implicitly modeled by an empirical function and the membrane properties are modeled by energetic restraints. The ability of each rhamnolipid structure to form an assembly with several PPM constituents (phospholipid (PLPC), sterols (Sitosterol, Stigmasterol, Campesterol) and sphingolipids (GIPC, Glucosylceramide)) was calculated using the HYPERMATRIX procedure, which calculate and minimize the energies of interaction between all molecules of the complex until the lowest energy structure is reached. [less ▲]

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See detailRole of non-polyQ regions on the aggregation process by polyQ proteins into amyloid fibrils
Huynen, Céline ULg; Willet, Nicolas ULg; Buell, Alexander K et al

Poster (2014, October 18)

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated ... [more ▼]

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated proteins. This polyQ expansion is thought to be the major determinant in the development of neurotoxicity, triggering protein aggregation into amyloid fibrils. A large body of evidence however indicates that non-polyQ regions modulate the aggregation process triggered by polyQ expansions. The interplay between the polyQ tract and non-polyQ regions is complex and still not fully understood. In order to better understand it, we previously designed and characterized model polyQ proteins made of the beta-lactamase BlaP and a 23, 30, 55 or 79Q tract inserted in position 197 or 216. These chimeras recapitulate the aggregation properties of polyQ disease-associated proteins: there is a Q-threshold for the formation of amyloid fibrils, and above the threshold, the longer the polyQ, the faster the aggregation. Moreover, the structure of BlaP (native or unfolded) and the position of insertion of the polyQ tract (197 versus 216) influence their aggregation properties. In this work, (i) we discuss the role of the conformation of the host protein, BlaP, and of the location of the polyQ within BlaP on the different phases of amyloid fibril formation, the nucleation and elongation steps, using mainly quartz crystal microbalance (QCM), atomic force microscopy (AFM). Our results highlight a linear dependence of the polyQ length on the elongation rate whatever the insertion site and the conformation of BlaP. These two parameters however drastically influence the ability of a polyQ tract to trigger the nucleation and the elongation steps of amyloid fibril formation. (ii) Finally, we investigate the precise aggregation threshold and the modulating role of the N- and C-terminal polyQ flanking sequences in position 197 of BlaP by creating and characterizing new chimeras containing intermediate length polyQ tracts in position 197, or polyQ tracts inserted between two cleavage sites in position 197, respectively. We observe that the propensity to trigger the full process of amyloid fibril formation and its rate seems to be largely dependent on the polyQ length and on the polyQ flanking sequences. Altogether our results contribute to identify the important species and elements (polyQ or non-polyQ regions, monomers, oligomers or fibrils) during the aggregation process into amyloid fibrils to interfere with the latter associated with neurotoxicity. [less ▲]

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See detailEffect of bovine colostrum on growth and survival in Red kids during the first year of life.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

Poster (2014, October 17)

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted ... [more ▼]

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted in Secondary Goat Breeding Center of Maradi (SGBCM). Forty newborn kids, regardless of sexe, were divided randomly into two groups: a "T-Control " group receiving only breast milk per feeding (n = 20) and a "C-Colostrum" group having the same diet but supplemented with bovine colostrum thawed (50 ml the first day of life, then 25 ml in 2 meals/ day between the ages of 2 and 15d, n = 20). The results indicate that supplementation with bovine colostrum in the first 15 days of life, increases the growth of kids until weaning (P<0.001), modifie some barymetric changes some settings improves health status and reduces the mortality rate. To our knowledge, in the studied environment, this work is original and seems to bring new knowledges likely to have practical applications in areas with farming conditions quite difficult. [less ▲]

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See detailMélancolie et dépression : étude phénoménologique
Ramackers, Amélia; Englebert, Jérôme ULg

Poster (2014, October 17)

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See detailLocal goat in Kabylie (Chemini and Bouzeguene)
Moula, Nassim ULg; Michaux, Charles ULg; Philippe, François-Xavier ULg et al

Poster (2014, October 17)

The livestock sector plays a dynamic role in the economic development of rural areas in Algeria. Due to its adaptation to harsh environments, goat breeding is practiced in many areas of the country. To ... [more ▼]

The livestock sector plays a dynamic role in the economic development of rural areas in Algeria. Due to its adaptation to harsh environments, goat breeding is practiced in many areas of the country. To the north, it is confined to mountainous areas, but the bulk of the workforce is left in the steppe and semi-desert areas. The goat population in Algeria reached 3.8 million heads, ranking second after sheep. The present work contributes to a better characterisation of local goat in Kabylie for production and reproduction traits. The study was conducted in the regions of Chemini (Bejaia province) and Bouzeguene (Tizi-Ouzou province). A survey was conducted in 29 herds. The results revealed that the overall mean of goats per households was 6.69 ± 2.97. The purposes of keeping goat in Chemini and Bouzguene was private consumption of milk (82.76%) and meat (68.97%) as well as for commercial exchange (62.07%). The Kabyle goat is small (male: 62.23 cm and female: 58.41 cm) with long hair. Its ears are drooping, its convex profile has a slightly pronounced nasal split and its coat colour ranges from dark brown to black. However, the crossbreeding with exotic breeds (mainly with Saanen goat), controlled or uncontrolled, increased the frequency of white coat. The average age at puberty in males was 6.93 ± 1.04 and 7.38 ± 0.94 months in females. The reported age at first kidding and kidding interval were 13.03 ± 0.87 months and 7.83 ± 1.29 months, respectively. [less ▲]

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See detailThe Effect of Urtica dioica Supplements on Egg Quality
Touazi, Leghel; Moula, Nassim ULg

Poster (2014, October 17)

Eggs are a rich source of lipids and proteins. Because of their low production cost, they represent a cheap source of animal proteins and lipids. recently, in Algeria, consumers’ interest are oriented of ... [more ▼]

Eggs are a rich source of lipids and proteins. Because of their low production cost, they represent a cheap source of animal proteins and lipids. recently, in Algeria, consumers’ interest are oriented of healthiness and dietetic values food. The purpose of this study was to investigate the effects a dietary supplement of Urtica dioica on laying hen's performance and egg quality. The study was conducted from may to june 2014 in the area of Chemini (Algeria). Sixty, 25-wk-old Lohmann Brown layers were randomly divided into 3 groups with 20 hens in each group. Group 1 was the control group fed a standard commercial diet based on corn and soybean, whereas the groups 2 and 3 were fed the control diet supplemented with 1% and 1.5% of nettle respectively. The hens were housed in cages in the same local and fed ad libitum. Fifty five eggs were sampled 4 times in each group from 25 to 27 weeks. A total of 220 eggs were examined. A series of measurements were carried out on each egg, namely egg weight, form index (egg shape), yolk to albumen ratio (Y:A), Yolk color, egg shell thickness, Haugh’s units. Egg weight, yolk color and Haugh Units (HU) were affected by age. Significant differences (P<0.05) were recorded between feeding type in total egg weight, yolk color and Haugh Units. The egg from group 2 proved to have a higher egg weight (57.67g; group 1: 55.41g; group 3: 56.70g; P<0.05), (33.4%), intermediate Yolk color (8.53; group 1: 9.03 ; group 3: 9.38; P<0.05) and HU (79.81; group 1: 80.85; group 3: 76.92). The results showed that the use of 1% and 1.5% of Urtica dioica, had positive effects on the color of yolk of laying hens. Using different levels of Urtica dioica could change the characteristics of egg and could meet the preferences of consumers for the intense color of the yolk. [less ▲]

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See detailEffet de l'expertise musicale sur la perception de la justesse vocale
Gosselin, Laura; Morsomme, Dominique ULg; Larrouy, Pauline ULg

Poster (2014, October 17)

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See detailThe effect of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler
Moula, Nassim ULg; Philippe, François-Xavier ULg; Michaux, Charles ULg et al

Poster (2014, October 17)

The objective of this study was to evaluate the effects of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler chicken. The study was ... [more ▼]

The objective of this study was to evaluate the effects of the inclusion of Urtica dioica leaves meal in diets on growth performances and carcass characteristics of broiler chicken. The study was conducted from May to June 2014 in the area of Chemini (Algeria). In a completely randomized design, a total of 100 one-day-old broilers (industrial strain) were divided into 2 groups and 2 repetitions with 25 chickens each. Group 1 was the control group fed a standard commercial diet based on corn and soybean purchased on the local market, whereas the birds in group 2 were fed the nettle diet (control diet with addition of 2% of nettle). The animals were housed inside in experimental pens and fed ad libitum. The results showed that nettle inclusion significantly promotes growth performance of broilers at 42 days of age (1644.8 ± 45.5 vs. 1565.1 ± 45.5g; P<0.05). The broilers fed the nettle diet had higher thigh yield (26.0 ± 0.3 vs. 25.2 ± 0.3%; P<0.05) but lower abdominal fat yield (4.39 ± 0.16 vs. 3.90 ± 0.16%; P<0.05). No significant effect of the diet was observed for carcass yield (around 71.1%) and breast percentage (around 32.40%). The overall mortality was not significantly different (P>0.05) for any of the dietary regimens (around 20%). Feed conversion ratio of the two groups were very close (around 2.17). This experiment showed that dietary inclusion of Urtica dioica has positive effects on growth performance and carcass quality of broilers. [less ▲]

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See detailPhenotyping Belgian Blue cattle for their susceptibility to psoroptic mange
Abos, Romain ULg; Coussé, Annelies; Sarre, Charlotte et al

Poster (2014, October 17)

Detailed reference viewed: 12 (1 ULg)
See detailVascular endothelial growth factor: a blood biomarker in canine idiopathic pulmonary fibrosis.
Roels, Elodie ULg; Krafft, Emilie ULg; Laurila, HP et al

Poster (2014, October 17)

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See detailL'intolérance environnementale idiopathique attribuée aux champs électromagnétiques (IEI-EMF)
Demaret, Isabelle ULg

Poster (2014, October 16)

Depuis le début des années '80, des chercheurs étudient les plaintes des personnes se disant hypersensibles aux champs électromagnétiques (EMF), sous les normes recommandées. Les recherches n'ont ... [more ▼]

Depuis le début des années '80, des chercheurs étudient les plaintes des personnes se disant hypersensibles aux champs électromagnétiques (EMF), sous les normes recommandées. Les recherches n'ont cependant pas démontré que ces personnes étaient capables de détecter les EMF ni qu'elles présentaient des changements physiologiques en étant exposées aux EMF . L'OMS a regroupé ces plaintes sous le nom d'Intolérance Environnementale Idiopathique attribuée aux Champs Electromagnétiques (IEI-EMF). Au niveau médical, l'IEI-EMF est classée parmi les syndromes fonctionnels somatiques comme d'autres syndromes sans bases physiologiques démontrées. [less ▲]

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See detailIncrease in northern hemisphere stratospheric hydrogen chloride over recent years
Mahieu, Emmanuel ULg; Chipperfield, MP; Notholt, J et al

Poster (2014, October 07)

Detailed reference viewed: 14 (2 ULg)