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See detailLes limites de l’administration de la PCL - R à une population de sujets schizophrènes dans le contexte médico-légal
Englebert, Jérôme ULg; Matteucci, Marie; De Page, Louis et al

Poster (2017, May 31)

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See detailBelgian speleothem records Holocene cold events?
Allan, Mohammed ULg; Deliège, Adrien ULg; Nicolay, Samuel ULg et al

Poster (2017, May 12)

Speleothem is now regarded as valuable archive of climatic conditions on the continents, offering a number of advantages relative to other continental climate proxy recorders such as lake sediments and ... [more ▼]

Speleothem is now regarded as valuable archive of climatic conditions on the continents, offering a number of advantages relative to other continental climate proxy recorders such as lake sediments and peat cores. High spatial resolution measurements of Mg, Al, Sr, Ba were realized by using laser-ablation inductively coupled plasma mass spectrometry in the Belgian Pere Noel cave. A stalagmite from the Pere Noel (PN) cave representing 12000 years dated by U/Th method. Trace element variations in speleothem are a reflection of hydrochemical conditions. These changes were interpreted as indications of changes in climate in the Han-sur-Lesse region. The similar patterns found in δ 18O, δ 13C and chemical composition along the Pere Noel stalagmite suggests that trace elements in speleothems have the potential to provide high resolution insights into palaeoclimatic variability during the Holocene. A deeper analysis reveals several periods of significant rapid climate change during the Holocene (at 10.7-9.2 ka, 8.2-7.9 ka, 7.2-6.2 ka, 4.8-4.5 ka, and 3-2.4 ka BP), which are similar to the cold events detected from different natural paleoclimate archivers. A comparison between the geochemical analysis of Père Noël speleothem and solar activity (sunspot number) reveals a significant correlation. Spectral analysis methods reveal common solar periodicities (Gleissberg cycle, de Vries cycle, unnamed 500 year, Eddy cycles, and Hallstatt cycle). The geochemical analyses have the potential to prove that PN speleothem is sensitive to changes in solar activity on centennial and millennial timescales during the Holocene. [less ▲]

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See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULg; Centonze, Prescilia ULg; Comblain, Fanny ULg et al

Poster (2017, April 27)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

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See detailTraits psychopathiques dans une population infra-clinique et traitement émotionnel
Englebert, Jérôme ULg; Bral, Laura; Dehon, Hedwige ULg et al

Poster (2017, April 19)

L’intérêt pour la psychopathie infra-clinique n’est pas neuf. Toutefois, les études sur le sujet sont encore peu nombreuses. Notre recherche a consisté à mettre à l’épreuve deux hypothèses. La première ... [more ▼]

L’intérêt pour la psychopathie infra-clinique n’est pas neuf. Toutefois, les études sur le sujet sont encore peu nombreuses. Notre recherche a consisté à mettre à l’épreuve deux hypothèses. La première, concerne la présence de « traits psychopathiques » dans la population tout-venant et la seconde tend à évaluer dans quelle mesure la présence de ces traits influence le traitement émotionnel. Pour tester ces hypothèses, nous avons créé une version « online » du SRP-III (Self Report Psychopathy). Aux soixante-quatre items initialement présents dans cette échelle nous avons ajouté dix-sept items afin d’être en mesure d’également coter la PCL-R (Psychopathy Check List- Revised). Deux groupes de chacun treize participants ont été créés sur base de leur score au SRP-III (un groupe « faibles caractéristiques psychopathique » et un groupe « fortes caractéristiques psychopathiques »). La seconde partie de l’étude consistait en une entrevue durant laquelle nous avons procédé à des mises en situation d’induction émotionnelle. Pour mesurer l’influence de cette induction, trois tâches cognitives étaient administrées aux sujets des deux groupes. La première hypothèse s’est vue confirmée à travers la récolte des données du questionnaire en ligne. Quant à la seconde hypothèse, les résultats statistiques n’indiquent aucune différence significative entre les groupes en ce qui concerne leur score aux tâches cognitives. Il est donc suggéré que la présence de traits psychopathiques n’influence pas le traitement émotionnel. Cette recherche confirme la présence de la psychopathie dans la population tout-venant et semble infirmer, parmi cette population, l’hypothèse d’un déficit du traitement émotionnel. [less ▲]

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See detailEvaluation des traits psychopathiques dans une population de sujets schizophrènes : Les limites de la PCL-R
Englebert, Jérôme ULg; Matteucci, Marie; De Page, Louis et al

Poster (2017, April 19)

Notre étude vise à déterminer si la PCL-R (Psychopathy Check List- Revised), destinée à évaluer la psychopathie et utilisée dans le contexte médico-légal, est adaptée à des sujets diagnostiqués ... [more ▼]

Notre étude vise à déterminer si la PCL-R (Psychopathy Check List- Revised), destinée à évaluer la psychopathie et utilisée dans le contexte médico-légal, est adaptée à des sujets diagnostiqués schizophrènes. Notre hypothèse principale est que plusieurs items de l’échelle peuvent être côtés avec le même score et pourtant exprimer une manière d’être et d’agir différente si le sujet est réellement psychopathe ou s’il est schizophrène. Afin d’éprouver notre hypothèse, nous avons sélectionné sept patients présentant soit une schizophrénie, soit une personnalité psychopathiques, soit considérés comme potentiels « héboïdophrènes » (schizophrénie pseudo-psychopathique) à qui nous avons administré la PCL-R. À partir d’analyses qualitatives, nous avons ensuite établi une liste d’items de la PCL-R sensibles à la psychose. Nous observons que 45% des items se révèlent positivement ou négativement influencés par la psychose au-delà de tout trait psychopathique. Sémiologiquement, c’est la présence de la dynamique paranoïde de la schizophrénie qui influence principalement les scores attribués aux items de la PCL-R. Cette recherche suggère des hypothèses concernant l’interaction psychose-psychopathie et son rapport avec des actes de violence. La conclusion de l’étude est que la PCL-R connait des limitations importantes en ce qui concerne son application à une population de sujets schizophrènes. [less ▲]

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See detailRecognition memory is associated with hippocampal volume in aging population: new evidence from brain imaging study
Narbutas, Justinas ULg; Blanpain, Manon ULg; Van Egroo, Maxime ULg et al

Poster (2017, March 23)

Introduction. The hippocampus is crucial for episodic memory, especially for recollection and pattern separation (i.e., the ability to store similar episodes as distinct memory traces). Episodic memory ... [more ▼]

Introduction. The hippocampus is crucial for episodic memory, especially for recollection and pattern separation (i.e., the ability to store similar episodes as distinct memory traces). Episodic memory declines with aging and this has been associated with hippocampal dysfunction. The main objective of our study was to explore how performance on a recognition memory task designed to assess pattern separation is associated with hippocampal volume in aging population. Methods. Fourteen healthy late middle-aged participants (52-69 years-old) were evaluated on a Mnemonic Similarity Task (MST). In this task, participants study pictures and then have to discriminate between targets, similar lures, and unrelated foil objects. Recognition memory (RM) is assessed as the difference between hits and false alarms to unrelated foils, while pattern separation Bias metric (BPS) is the difference between the rate of ‘‘Similar’’ responses given to the lure items minus ‘‘Similar’’ responses given to the foils. Hippocampal volume was calculated using ASHS software, which uses T1-weighted and T2-weighted MRI to obtain optimal segmentation of hippocampal subfields. Results. Correlation analysis of preliminary data revealed that RM was significantly positively associated with the volume of the left subiculum and left perirhinal area 35, while there were no significant correlations in the right hemisphere. BPS was positively correlated with the volume of right CA2 region, but negatively associated with the volume of right CA3 region, what is more contradictory according to the current literature. No significant link was found between BPS and the volume of hippocampal subfields in the left hemisphere. Conclusion. In a middle-aged population, better visual recognition memory is associated with larger volume of the left subiculum and perirhinal area 35, two regions supporting representation of objects [less ▲]

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See detailINVESTIGATION DE LA QUALITE D’ANTIBIOTIQUES A BASE D'AMOXICILLINE DANS LE CADRE DE LA SURVEILLANCE DU MARCHE OFFICIEL ET PERIPHERIQUE DE QUELQUES VILLES DE LA R.D. CONGO
Kalenda Tshilombo, Nicodème ULg; Ciza Hamuli, Patient ULg; Mavungu Landu, Don Jethro ULg et al

Poster (2017, March)

Drug counterfeiting is a sad and worrisome reality, especially in developing countries where quality control is not effective and sometimes not existing at all despite political will of governments. The ... [more ▼]

Drug counterfeiting is a sad and worrisome reality, especially in developing countries where quality control is not effective and sometimes not existing at all despite political will of governments. The consequences are harmful in particular for substandard medicines that pose more threats to populations in those countries due to their direct negative impact on patients such as failure of medical treatment including development of drug resistance and even death. Socio-economic consequences and negative reputation concerning the pharmaceutical industry are also observed. Unfortunately accurate detailed data on such medicines are not easy to obtain. Most of the time available data are often estimated from case reports or studies carried out in a specific area and during a defined period.Health authorities’ in the Democratic Republic of Congo are trying to identify this scourge by set up several strategies to fight against. One of them is built on the best knowledge of drugs from several horizons through the assessment of their quality to allow appropriate measurement. In this context, we have focused our study towards amoxicillin alone and/or combined with potassium clavulanate since it is one the very used medicines in pediatric medications. The formulations are powder for suspension. Two analytical methods were developed based on the USP monography, applying isocratic liquid chromatography. Prior to their application in routine, we evaluated the suitability of these methods through validation applying the accuracy profile of total error. Since it was planned to transfer the methods to DRC, several operating factors were taken into account namely operator, day and equipment. Interesting results were obtained in terms of trueness (relative biases below than 2.3%), precision (RSD of Intermediate precision below 2.8%), accuracy (beta-expectation tolerance intervals between -6.0% and 3.8%) for the concentration levels of interest. The latter were able to allow monitoring the quality of the two active ingredients here above in the 65 samples from Congolese market. They were collected in Kinshasa, Lubumbashi, Matadi and Kolwezi at official and non-official medicines distributors, in peripheral area. The dramatic results obtained confirm that substandard and counterfeit medicines remain a crucial problem on public health in low-income countries. Appropriate measures are really needed to set up the drug quality improvement. [less ▲]

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See detailImportance of using Geographic Information System for extensive Middle Palaeolithic open air sites in northern France. The example of Caours (Somme, France) and Beauvais (Oise, France).
Moreau, Gwénaëlle ULg; Locht, Jean-Luc; Patou-Mathis, Marylène et al

Poster (2017, February 09)

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement ... [more ▼]

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement, raising complication in the study of its dynamics at a regional scale. Therefore, some aspects of Neanderthals behaviour are still mischaracterize: in most cases, the function of the site and its territorial management over the northern France have to be specified. The spatial analysis of open air sites from northern France will help us to answer those questions. However, for Middle Palaeolithic sites, we can't see systematically on the field the spatial organisation directly, we sometimes need modelization. Therefore, we started to build a spatial analysis protocol adapted to this site and applied to such sites. The sites of Caours (Somme, France) and Beauvais (Oise, France) are two open air sites that are exceptionally well preserved and displaying a large amount of faunal and lithic rests. Both are perfect candidate to apply and test the new protocol. First results proved that for each site the existence of a spatial organisation as remains concentration zones. Then, we characterize this areas – number, distribution. Finally, we were able to associate them to human activity areas like hearths, butchery or knapping areas. [less ▲]

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See detailLab Pay(S)age: a Laboratory of Observation as an activator for Landscape Awareness Process
Baldin, Elisa ULg; Occhiuto, Rita ULg

Poster (2017, February 09)

The notion of Landscape Observatory (ELC 2000), in the work of LabVTP at the Faculty of Architecture in the University of Liège (Wallonia-BE), has been linked with the concept of Landscape Laboratory (R ... [more ▼]

The notion of Landscape Observatory (ELC 2000), in the work of LabVTP at the Faculty of Architecture in the University of Liège (Wallonia-BE), has been linked with the concept of Landscape Laboratory (R. Gustavsson) in order to enhance and underline perceptive, experiential and active dimensions of sites, responding to the Universities’ missions required by the ELC: education and sensitization. Lab Pay(s)age, as a new typology of landscape laboratory, has developed methodological tools for landscape research, based on a multi-scale and diachronic reading of the territory, where natural and artificial traces are the complementary elements to be reinterpreted and relaunched in new participative scenarios. The Meuse valley, reshaped by deep transformations, is the main topic for researches, concerning specific morphological, typological and social characters. All along the river, the scars of industries mark the territory as a land in search of new cycle of life1. The regeneration of abandoned industrial sites, as a practice of territorial recycle, is part of sustainability policies in urban planning, based on the enhancement of local resources. The Observatory Lab Pay(s)age, is intended as a structure for site-specific study and continuous fieldwork, concerning both the evolutionary processes of natural structures and the involution of built environment. Starting from these materials, many different regeneration methodologies and temporalities have been imagined. Following the logic promoted by the ELC, the work has started by monitoring some specific areas and by contacting different local representatives with the aim of collecting data and experiences to construct a shared knowledge of the places. Then the project has become an on-going tool including all phases in the regeneration process: observation, analysis, interpretation and re-launch of new hypothesis are fundamental elements to increase the public knowledge about the cultural factors characterizing the landscape discipline. Thus, the notion of « observatory » is a strategic device, monitoring the evolution of places in time through actions of dialogue and awareness raising, in order to get citizens re-acquire a consciousness of their territory. The collected qualitative data, describe the peculiar features of the sites, based on the local actors experience and knowledge. These elements are the basis for new scenarios, used as progressive steps leading the recovery process. For this reason, the « observatory » is also a « laboratory », as the experimental projects accompany the transformation of the places through intermediate phases. Understanding and accepting mutability as a character of landscape is a preliminary step towards a co-action, where the project accords with natural dynamics. Lab Pay(s)age, according to the ELC principles, is using the earliest local answers as re-launch factors for consecutive projects, taking into account the territorial temporalities as priority in transformation process. The presentation concerns the ongoing projects and obtained reactions, such as the earliest re-naturalization programs, some selected sites for monitoring activities and Phd researches on new regeneration methods using vegetation as an active element in reclamation of industrial sites. 1 Occhiuto, R. 2016. Healing the scars of industry in Liège, in Scape n.15, 2016 [less ▲]

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See detailNew data on the Mesozoic radiation of chelonioids
Scavezzoni, Isaure ULg; Fischer, Valentin ULg

Poster (2017, February)

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See detailMegaloolithid dinosaur eggs: scrambled parataxonomy and nesting strategies
Jentgen, Benjamin ULg; Stein, Koen; Fischer, Valentin ULg

Poster (2017, February)

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See detailThe Bright and Dark Sides of elliptical galaxies
Biernaux, Judith ULg

Poster (2017, January 29)

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See detailChildren’s engagement in physical education could be improved by stories and imagination.
De Sousa Morgado, Liliane ULg; Jidovtseff, Boris ULg

Poster (2017, January 27)

Introduction: The development of fundamental motor skills is essential during childhood and should be a learning outcome in the physical education (PE). With the children between 3 to 6 years old it could ... [more ▼]

Introduction: The development of fundamental motor skills is essential during childhood and should be a learning outcome in the physical education (PE). With the children between 3 to 6 years old it could be sometime difficult to get their attention and motivation during PE lessons. Teachers have to use a very adapted pedagogical approach in order to get all children’s engagement. Experience: The CEReKi is a research center of the University of Liège (Belgium) that has developed adapted activities where preschools children are playing and at the same time developing perceptuals and fundamental motor skills. From our field experience one of the more efficient ways to get children attention and participation is to introduce the activities with stories and imagination. Teachers from CEReKi are using fantasy in order to invite children into a funny world where everything could become possible like the "the never-never land of Peter Pan". The way of telling the story as well as the used characters (like the wolf) must be adapted to the age of the children. Critical analysis: From our experience, using stories and imagination is effective in increasing children‘s engagement during PE as it makes activities more fun and more enjoyable. Even if we don’t have any measurement on its effect, we are convinced that such approach improve children’s motor learning. However using stories and imagination with the children is neither a natural nor an easy thing for inexperienced teachers. The use of fantasy with the children should be introduces in the vocational training. Conclusions: With more than twenty years of experience, we believe that stories and imagination are powerful factors that makes physical activities funny and enjoyable for the children. Such strategy may positively enchase young people’s attitudes towards physical education and ultimately, physical activity participation. [less ▲]

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See detailGlobal Simulation of UV atmospheric Emissions
Gonzalez-Galindo; Lopez-Valverde; Forget et al

Poster (2017, January 19)

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See detailA persistent meteoric layer in Mars' atmosphere
Crismani; Schneider; Plane et al

Poster (2017, January 18)

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See detailThree Types of Aurora observed by MAVEN/IUVS: Implications for Mars’ upper Atmosphere Energy Budget
Connour; Schneider; Jain et al

Poster (2017, January 17)

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See detailThe added value of plasma or urinary NGAL concentrations in clinical practice
Gregoire, Emilien ULg; Claisse, Guillaume; GUIOT, Julien ULg et al

Poster (2017, January 13)

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See detailIron, zinc and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2017, January 12)

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See detailMacroevolutionary patterns in sea breams, emperors and allies (Sparoidea, Acanthomorpha)
Santini, Francesco; Olivier, Damien ULg; Frederich, Bruno ULg

Poster (2017, January 07)

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See detailRaman imaging for diagnostic application
Malherbe, Cédric ULg; Gilbert, Bernard ULg; Eppe, Gauthier ULg

Poster (2016, December 19)

Specific dynamic biochemical compositions and molecular structures characterise healthy cells and tissues. This also stands for pathologies or cellular abnormalities which are accompanied by biochemical ... [more ▼]

Specific dynamic biochemical compositions and molecular structures characterise healthy cells and tissues. This also stands for pathologies or cellular abnormalities which are accompanied by biochemical and molecular changes. Raman spectroscopy can provide unique inputs to correlate the molecular composition and its variations with the diagnosis. In addition, the presence of metal nanoparticles in the molecule vicinity induces a remarkable enhancement (up to 1000000 times) of the Raman signal of small molecules (known as SERS effect). It opens towards applications in the field of trace analysis. In preparation for clinical applications, we evaluate, by Raman mapping, the signal detected from a SERS target deposited on solid state support. [less ▲]

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See detailIntegrated microfluidic device for the synthesis of SERS substrates
Emonds-Alt, Gauthier ULg; Malherbe, Cédric ULg; Monbaliu, Jean-Christophe ULg et al

Poster (2016, December 19)

The goal of this work is to develop a quantitative analytical method for the glyphosate and its major metabolite the aminomethylphosphonic acid (AMPA) by coupling microfluidics with surface enhanced Raman ... [more ▼]

The goal of this work is to develop a quantitative analytical method for the glyphosate and its major metabolite the aminomethylphosphonic acid (AMPA) by coupling microfluidics with surface enhanced Raman spectroscopy (SERS). This work is divided into 4 axes : (a) bimetallic nanoparticles synthesis in a microreactor, (b) nanoparticles functionnalisation, (c) SERS quantification of glyphosate/AMPA and (d) miniature prototype development composed of axes a to c. The first section aims at the synthesis of bimetallic nanoparticles (Au-Ag, Au-Cu and Ag-Cu) in a microfluidic device. This microfluidic device integrates a first section for the synthesis of monometallic nanoparticles (core) and a second section for the reductive deposition of a second metal (shell). The second axe of this work deals with the functionnalisation of nanoparticles to improve the selectivity of this method for the selected analytes. Two types of functionnalisation are studied : PEGylation (a) with multi-arm PEG ending with -SH and/or (b) with linear heterobifunctionnal PEG. The third axe consists in measuring quantitatively glyphosate and AMPA in different matrices, such as water, urine and fruit juice. Finally, the forth axe deals with the development of a portable detection system to perform on-site analysis. This prototype integrates the microfluidic device for nanoparticles synthesis, their functionnalisation, an injection port followed by a mixing section for the sample and an integrated Raman spectrometer for in-line analysis. [less ▲]

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See detailPrésentation du projet Economie et pratiques des sociétés gravettiennes dans le Nord-Ouest de l’Europe (ECOPRAT)
Touzé, Olivier ULg; Salomon, Hélène ULg; Goutas, Nejma et al

Poster (2016, December 17)

Nous présentons dans cette contribution un projet collectif porté par le Service de Préhistoire de l’Université de Liège et l’équipe Ethnologie préhistorique de l'unité mixte de recherche du CNRS ... [more ▼]

Nous présentons dans cette contribution un projet collectif porté par le Service de Préhistoire de l’Université de Liège et l’équipe Ethnologie préhistorique de l'unité mixte de recherche du CNRS Archéologie et Sciences de l'Antiquité. Le projet « ECOPRAT » entend contribuer au renouvellement des connaissances relatives aux sociétés gravettiennes du Nord-Ouest européen, et prolonger ainsi la dynamique de recherche actuelle touchant ces sociétés (Bodu et al., 2013). Il s’inscrit dans une perspective pluridisciplinaire envisagée grâce à l’association d’études technologiques, tracéologiques et archéozoologiques. Quatre sites majeurs du Nord-Ouest européen ont été retenus : les gisements de plein air de Maisières-Canal (Prov. Hainaut, Belgique) et d’Ormesson – Les Bossats (Seine-et-Marne, France), et les grottes de Goyet (Prov. Namur, Belgique) et du massif d’Arcy-sur-Cure (Yonne, France). Les sites sélectionnés possédant des historiques de recherche spécifiques, la stratégie d’analyse adoptée pour chacun d’eux est développée en adéquation avec l’état des connaissances actuelles. Les recherches sont ainsi guidées par l’obtention de données inédites qui doivent permettre, à terme : 1) une lecture renouvelée et croisée des industries lithiques et osseuses, ainsi que des ensembles fauniques et des matières colorantes découverts dans ces gisements, 2) un rééquilibrage quantitatif et qualitatif de l’intégration des différentes sources documentaires disponibles, la recherche s’étant davantage concentrée depuis plus d’un siècle sur les données de l’industrie lithique. Les deux gisements de plein air réunissent les conditions indispensables au développement d’une approche interdisciplinaire permettant de croiser objets et méthodes d’étude, afin de proposer une lecture fine des comportements techniques et économiques des sociétés gravettiennes. A cet égard, cette contribution est l’occasion de présenter les premiers résultats obtenus sur le site de Maisières-Canal. Les recherches réalisées jusqu’à présent permettent de réévaluer l’importance de l’industrie sur matières dures d’origine animale, au sein de laquelle le travail de l’ivoire de mammouth se révèle particulièrement significatif. En outre, le corpus faunique traduit une exploitation récurrente du lièvre, ce type de comportement s’avérant singulier au regard de ce qui est habituellement documenté chez les sociétés du Gravettien. Parmi les différentes activités susceptibles de laisser une trace archéologique, l’usage de matières colorantes apparaît particulièrement discret dans le cadre spatio-temporel considéré. Cet usage est cependant avéré dans les grottes d’Arcy-sur-Cure (grottes du Renne et du Trilobite), lesquelles permettront ainsi d’entreprendre une étude ciblée de ce phénomène. Enfin, les grottes de Goyet offrent la possibilité de contribuer à la connaissance de la séquence gravettienne du Nord-Ouest européen. Si le long historique des fouilles menées dans ces grottes, ainsi que les mélanges manifestes affectant les collections, ne permettent pas d’envisager une étude approfondie des comportements gravettiens, des observations préliminaires permettent en revanche de s’interroger sur le potentiel de ces sites pour la reconstitution de cette séquence. A l’instar de ce qu’ont montré les travaux récemment conduits sur le Gravettien de Spy (Pesesse & Flas, 2013), il est en effet vraisemblable que les grottes de Goyet aient été occupées à plusieurs moments de la période gravettienne. BODU P., CHEHMANA L., KLARIC L., MEVEL L., SORIANO S., TEYSSANDIER N. (dir.), 2013. Le Paléolithique supérieur ancien de l’Europe du Nord-Ouest : réflexions et synthèses à partir d’un projet collectif de recherche sur le centre et le sud du Bassin parisien, Actes du colloque de Sens, 15-18 avril 2009. Paris : Société préhistorique française (Mémoires de la Société préhistorique française, 56), 516 p. PESESSE D., FLAS D., 2013. Which Gravettians at Spy? In : ROUGIER H., SEMAL P. (eds), Spy Cave. 125 years of multidisciplinary research at the Betche-aux-Roches (Jemeppe-sur-Sambre, Province of Namur, Belgium). Volume I. Bruxelles : Institut royal des Sciences naturelles de Belgique, Société royale belge d'Anthropologie et de Préhistoire, NESPOS Society (Anthropologica et Praehistorica, 123), pp. 257-268. [less ▲]

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See detailBioaccumulation of trace metals in Mytilus galloprovincialis from the Algerian west coast
Rouabhi, Leila; Rouanne, Hassen Omar; Benkrama, Hadjer et al

Poster (2016, December 16)

Algeria has a 1622 km long coastal strip where a large proportion of the population and the main economic and industrial activities of the country are concentrated. Its coastal fringe therefore suffers ... [more ▼]

Algeria has a 1622 km long coastal strip where a large proportion of the population and the main economic and industrial activities of the country are concentrated. Its coastal fringe therefore suffers from various degradations. In particular, the Bay of Oran is housing industrial, commercial, fishing and recreational activities, where 80 % of domestic and industrial wastewaters are not purified before being discharged into the sea. Thus, the aim of the present work was to evaluate the degree of metallic contamination of coastal waters of western Algeria. Trace metals (Zn, Cu, Ni, Fe and Pb) were measured by atomic absorption spectroscopy in the dry soft tissues (gills and gonads) of the mussel Mytilus galloprovincialis Lamarck, 1819 collected during the four seasons of year 2010 in 2 sites: the highly polluted Oran harbor (S1) and Ain Defla (S2), a site distant enough from Oran and presumed little contaminated. Oran harbor was globally more contaminated than Ain Defla, especially for Fe, Zn and Ni. However, Pb levels were higher at Ain Defla with concentrations up to 3.35 ± 0.25 ppm in the gills. Metal concentrations differed between organs. Fe, Zn and Cu were more accumulated in gills (46.91 ± 1.60 ppm, 25.6 ± 1.07 ppm, 2.68 ± 0.50 ppm, respectively) compared to gonads (29.06 ± 1.07 ppm, 21.76 ± 1.46 ppm ppm, 1.44 ± 0.20 ppm, respectively). Each metal followed a seasonal trend, showing concentration peaks during winter and spring for gills and autumn for gonads. This study demonstrated the need to biomonitor the metallic contamination of Algerian coasts. But such monitoring surveys, relying on organisms, will require consensual sampling and analytical protocols to avoid hazardous conclusions due to tissue speciation and accumulation seasonality. [less ▲]

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See detailExploring a Mediterranean mesozooplankton 13 year time-series.
Fullgrabe, Lovina; Richir, Jonathan ULg; Batigny, Antoine et al

Poster (2016, December 16)

Zooplankton plays diverse crucial roles within the marine ecosystem and can also be used as bio- indicator of climate changes since it is very sensitive to environmental changes. Therefore it is essential ... [more ▼]

Zooplankton plays diverse crucial roles within the marine ecosystem and can also be used as bio- indicator of climate changes since it is very sensitive to environmental changes. Therefore it is essential to consider long-term plankton series. Given the analysis of plankton samples is time- consuming, it requires an effective and rapid analytical method. We have used in this work a supervised learning approach adapted for the semi-automatic classification of digital images of the mesozooplankton of the Bay of Calvi (Corsica, France) by using the Zoo/PhytoImage software. Together with a 11-years long zooplankton time-series, a set of nine environmental variables were monitored in order to identify controlling factors and determine whether the communities were sensitive to global environmental changes. The main components of the mesozooplankton community were characterized by both seasonal and inter-annual variability. Additionally, variation of holoplankton and meroplankton differentiated one from each other. The holoplanktonic community could be split into two subgroups according to its variation in function of the environment: cladocerans and appendicularians, and to a lesser extent, copepods on one hand, and cnidarians, chaetognathes and thaliaceans, on the other hand. Regarding inter-annual variation, one year (2007) showed particularly low production of total zooplankton which was also the case for all the different holoplanktonic taxa. Accounting for that phenomenon were identified some potentially underlying environmental factors. Finally, although water temperature increased significantly over the last years along with the frequency of marine heat wave events, no evident change in the global zooplankton composition was observed yet. [less ▲]

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See detailDetermining the Substorm Onset Instability Using Ground and Space-Based Measurements
Rae, Jonathan; Watt, Clare; Murphy, Kyle et al

Poster (2016, December 16)

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See detailCopper toxicity on coral holobiont photosynthetic processes
Georges, Nadège; Richir, Jonathan ULg; Batigny, Antoine et al

Poster (2016, December 16)

Copper (Cu), an essential micronutrient to organisms, may become toxic when present at too high environmental concentrations. This metal remains an aquatic contaminant of concern, notably because of its ... [more ▼]

Copper (Cu), an essential micronutrient to organisms, may become toxic when present at too high environmental concentrations. This metal remains an aquatic contaminant of concern, notably because of its recent re-use as biocide in metal-based antifouling paints. The aim of this study was to monitor the physiological alterations in a zooxanthellate coral species and its endosymbionts (i.e. the coral holobiont) exposed to increasing Cu concentrations. Nubbins of Seriatopora hystrix were exposed for 8 days in 1 L intermittent respirometers to 5 nominal Cu concentrations: 0-2-5-15-50 ppb. Respirometers were maintained at 25.0±0.2°C with successive open/close cycles of 30 min. A 12/12 hours day-night light regime was applied with constant daylight intensity of 200 μmol photons m-2 s-1. Water renewal rate during the 30 min open cycles was 15 mL.min-1. The photosynthetic performances of coral endosymbionts were assessed daily with a fluorescence imaging system (imaging-PAM). At the end of the 8-days experiment, the maximal photochemical quantum yield (FV/FM) of coral nubbins had decreased by 12% and 38%, respectively, in the 15 ppb and 50 ppb treatments. This decrease was even greater for the effective photochemical quantum yield (ɸPSII) with values dropping by 41% and 54%, respectively. Cu exposure also affected the symbiosis between the coral host and its endosymbionts. Nubbins of the 15 ppb treatment slightly lightened from day 6, whilst nubbins exposed to the 50 ppb treatment lightened from day 3, and started to bleach from day 6. The analysis of nubbins’ primary productivity did not coincide with the above observations, the oxygen production within each respirometer remaining relatively constant during the overall experiment for all treatments. This unexpected observation may be the sign of a compensation mechanism. In conclusion, Cu affected the photosynthetic processes of S. histrix within 8 days from relevant environmental concentrations of 15 ppb. The exposure of corals to toxic chemicals thus has to be considered as an additional stressor to, e.g., ocean acidification or elevated temperature, which may disturb their ecophysiology and lead to bleaching. [less ▲]

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See detailFeeding ecology of metamorphic and paedomorphic palmate newts (Lissotriton helveticus) in Larzac
Didaskalou, Emilie; Lejeune, Benjamin ULg; Lepoint, Gilles ULg et al

Poster (2016, December 16)

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See detailHead shape disparity of the cod icefishes Trematominae (Notothenioidei, Teleostei)
Frederich, Bruno ULg; Heindler, Franz M.; Dettai, Agnès et al

Poster (2016, December 16)

The suborder Notothenioidei (Teleostei) has undergone a remarkable adaptive radiation in the Southern Ocean. Within this suborder, the subfamily Trematominae is endemic to Antarctic waters and represents ... [more ▼]

The suborder Notothenioidei (Teleostei) has undergone a remarkable adaptive radiation in the Southern Ocean. Within this suborder, the subfamily Trematominae is endemic to Antarctic waters and represents a dominant component of the shelf fish fauna. After recent advances in molecular phylogenetics, 14 species of Trematomus are currently recognized (including Pagothenia and Cryothenia spp.) comprising both considerable morphological and ecological diversity. Here, we aim to illustrate the main axes of shape variation in Trematomus and explore the evolution of their morphology. A dataset of 96 specimens representing 10 species of Trematomus from the collection of the Natural History Museum of Paris was assembled, and landmark-based geometric morphometrics was applied to quantify head shape disparity. Regular regression analysis revealed significant interspecific allometry while a low percentage of shape variation was explained by size (R2 = 0.11; P < 0.001). Main shape variation across species was explored using a principal component (PC) analysis on shape variables. Two groups diverged along PC1: (1) T. bernacchii, T. hansoni, T. pennellii and T. tokarevi have short cephalic profiles with larger cheeks (lowest values along PC1); and (2) T. lepidorhinus, T. eulepidotus and T. newnesi show lengthened cephalic profiles with larger eyes (highest values along PC1). Trematomus scotti differed from all other species mainly along PC3 indicating more elongated cheeks. Phenogram based on Procrustes shape distances will be compared to molecular phylogenetic trees and morphometric data will be mapped onto phylogenetic trees in order to illustrate the mode of phenotypic diversification of Trematomus during evolution. [less ▲]

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See detailInsects complex associated with the tropical basil, Ocimum gratissimum L. (Lamiaceae) in southern Benin.
Yarou, Boni Barthélémy ULg; Bokonon-Ganta, H. Aimé; Assogba Komlan, Françoise et al

Poster (2016, December 16)

Tropical basil is an aromatic leafy vegetable used for its medicinal and therapeutic properties in numerous countries in West Africa (Benin, Nigeria, Togo, etc.). In Benin, it is produced on almost all ... [more ▼]

Tropical basil is an aromatic leafy vegetable used for its medicinal and therapeutic properties in numerous countries in West Africa (Benin, Nigeria, Togo, etc.). In Benin, it is produced on almost all urban and periurban garden throughout the year for fresh market. Until now there are few or nearly no publications about the arthropod community of this specie, even less in the context of Benin. Thus, to assess this community, basil plots were mowed using a sweep net in three localities (Ouidah, Togba and Sèmè) of southern Benin. Preliminary results focus on the different insect families that colonized tropical basil in southern Benin environmental conditions. These include: Aphididae (Homoptera), Cercopidae (Homoptera), Chrysomelidae (Coleoptera), Coccinelledidea (Coleoptera), Meloidae (Coleoptera), Braconidae (Hymenoptera), Ichneumonidae (Hymenoptera), Formicidae (Hymenoptera), Vespidae (Hymenoptera), Apoidae (Hymenoptera), Reduviidae (Heteroptera), Pentatomidae (Heteroptera), Pyrgomorphidae (Orthoptera), Acrididae (Orthoptera), Syrphidae (Diptera), Diopsidae (Diptera). Among these families, there are pests: Aphis gossypii G. (Homoptera: Aphididae), Zonocerus variegatus L. (Orthoptera: Pyrgomorphidae), etc.; predators: Ischiodon aegyptius W. (Diptera: Syrphidae), Cheilomenes spp. (Coleoptera: Coccinelledidea), Rhynocoris spp. (Heteroptera: Reduviidae), etc.; parasitoids and pollinators. From this study, it appeared that there is a large diversity of families and functional groups (pests, predators, parasitoids and pollinators) associated with tropical basil . Moreover, the presence of natural enemies could be an advantage for farmers in intercropping systems. This would help reduce the use of synthetic insecticides. This result, which is a first knowledge of the insect fauna associated with tropical basil under the environmental conditions of Southern Benin, will be supplemented by a spatio temporal study to assess the variability and the dynamics of this insect fauna. [less ▲]

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See detailEnergy Dissipation in Saturn’s Magnetotail: A Comparative Magnetotail Approach
Yao, Zhonghua ULg; Coates, Andrew; Ray, Licia et al

Poster (2016, December 16)

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See detailTuta absoluta (Lepidoptera: Gelechiidae) ability to develop on potato tubers
Bawin, Thomas ULg; De Backer, Lara ULg; Yarou, Boni Barthélémy ULg et al

Poster (2016, December 16)

Tuta absoluta (Lepidoptera: Gelechiidae) is a widespread pest that threatens commercial production of solanaceous crops. Larvae feed on the mesophyll of all aerial parts of the plants resulting in ... [more ▼]

Tuta absoluta (Lepidoptera: Gelechiidae) is a widespread pest that threatens commercial production of solanaceous crops. Larvae feed on the mesophyll of all aerial parts of the plants resulting in significant yield losses and cosmetic damages.It is classically assumed that underground plant parts are not affected but larval development on potato tubers has recently been suspected. However, this assumption is poorly supported in the literature. Potato tubers might represent an unsuspected mean of persistence and dispersal in agricultural environments.Oviposition tests were here conducted using dormant and active (sprouts less than 5 cm) potato tubers in net cages. Oviposition only occurred on the sprouts of active tubers, supporting larval growth until adult emergency. Tuber dissection revealed burrows starting from sprouts inside the flesh. Additional fitness assays were conducted on non-sprouted tubers and revealed that first instar larvae were able to enter the skin and create burrows, living inside and feeding on potato flesh, although none of them survived. [less ▲]

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See detailIntegrating Non-Collocated Well and Geophysical Data to Capture Lithological Heterogeneity at a Managed Aquifer Recharge and Recovery Site
Gottschalk, Ian; Hermans, Thomas ULg; Caers, Jef et al

Poster (2016, December 15)

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of ... [more ▼]

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of lithological heterogeneity at ARR sites often hinders attempts to predict where and how quickly infiltrating water will flow in the subsurface, which can adversely affect the quality and quantity of available water in the ARR site. In this study, we explored the use of electrical resistivity tomography (ERT) to assist in characterizing lithological heterogeneity at an ARR site, so as to incorporate it into a flow and contaminant transport model. In this case, we had non-collocated well core log data and ERT data from a full-scale ARR basin. We compared three independent methods for producing conditional lithology-resistivity probability distributions: 1) a search template to relate the nearest logged well lithologies with ERT resistivity panels, given search criteria; 2) a maximum likelihood estimation (MLE) to match bimodal normal distributions to the histogram of each ERT line; and 3) variogram-based lithology indicator simulations constrained to well data. Each approach leverages Bayes’ Rule to estimate lithology probability given electrical resistivity. The simplest approach (method 1) yields an erroneous conditional probability function where sand dominates the conditional probability at nearly all resistivities, due in part to the strong presence of sand in the wells nearest the ERT lines. The approaches using MLE and lithology simulations (methods 2 and 3) produce similar, more realistic lithofacies probability functions. The range of resistivities where clay and sand overlap differs between methods 2 and 3: ranging between 100 and 200 ohm-m for method 2, and between 30 and 50 ohm-m for the method 3. These differences affect the posterior lithology distributions in multiple point geostatistical (MPS) simulations, and in turn, predictions of flow from models which integrate these results. To test the models, we can compare measured breakthrough times of recharged water at the site to groundwater flow simulation results using the lithofacies models created by each method. The methods described here can inform the integration of non-collocated geophysical data into a variety of applications. [less ▲]

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See detailA step-by-step recipe of band-spli3ng technique for isola8on of ionospheric signal in L-band InSAR data
Furuya, Masato; Suzuki, Takato; De Rauw, Dominique ULg

Poster (2016, December 15)

Ionospheric signals are widely regarded as nuisance in low- frequency InSAR data, and thus a variety of correction techniques have been proposed. Although band-splitng of range spectrum has been shown to ... [more ▼]

Ionospheric signals are widely regarded as nuisance in low- frequency InSAR data, and thus a variety of correction techniques have been proposed. Although band-splitng of range spectrum has been shown to be effectve (Brcic et al., 2010; Rosen et al., 2010; Gomba et al., 2015), the band- spliting technique seems to have not been widely used. Whereas this would be partly because the impacts of ionosphere on InSAR data are not necessarily serious, we consider that this could be also because the details of the technique have not been reproducibly shown. Here we show our step-by-step recipe of the band-spliting technique, and demonstrate two case studies that reveal the effectiveness of the technique. [less ▲]

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Peer Reviewed
See detailElectrical Resistivity Monitoring of Heat Tracer to Characterize Lab-Scale Hydraulic Conductivity Distributions
Adetokunbo, Peter; Hermans, Thomas ULg; Oware, Erasmus

Poster (2016, December 14)

Knowledge of the spatial variations of hydraulic conductivity (K) is crucial to almost every hydrogeological investigation. The representative scale of K estimates from traditional slug and pumping tests ... [more ▼]

Knowledge of the spatial variations of hydraulic conductivity (K) is crucial to almost every hydrogeological investigation. The representative scale of K estimates from traditional slug and pumping tests are, however, inadequate to accurately predict hydrogeological processes. There is increasing interest in the application of electrical resistivity tomography (ERT) to quantify spatially continuous K variations. ERT estimation of high-resolution K distributions, however, requires continuous injection of saline tracer (ST) into an aquifer over an extended period, which is feasible but impractical. Here, we present electrical resistivity thermography (ERTh) to evaluate the potential application of time-lapse ER monitoring of heat tracer (HT) to characterize high-resolution K architectures. Unlike ST, long term HT experiments are comparatively easier to manage and repeatable with minimal environmental impact. We estimate K variations via petrophysical coupling of flow and heat transport with joint time-lapse ER and discrete multi-level temperature breakthrough curves. We illustrate the strategy with a 2-D lab-scale sandbox experiment. To construct the heterogeneous field, three lenses with high-K properties with each consisting of gravel, coarse sand, and a mixture of coarse and fine sand, were created within a background of comparatively low-K fine sand. The experiment involved continuous injection and extraction of heat, respectively, at the left and right boundaries of the lab-scale aquifer. We simultaneously performed time lapse ER monitoring of the heat transport and temperature measurements at four discrete multi-levels near the heat extraction well. Results of the coupled inversions demonstrate that ER monitoring of heat tracer provides a unique opportunity to characterize high-resolution spatially continuous K variations, which seems more practical for field applications in contrast to that of the traditional ST. [less ▲]

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See detailThe complex behavior of the satellite footprints at Jupiter: the result of universal processes?
Bonfond, Bertrand ULg; Grodent, Denis ULg; Badman, Sarah V. et al

Poster (2016, December 14)

At Jupiter, some auroral emissions are directly related to the electromagnetic interaction between the moons Io, Europa and Ganymede on one hand and the rapidly rotating magnetospheric plasma on the other ... [more ▼]

At Jupiter, some auroral emissions are directly related to the electromagnetic interaction between the moons Io, Europa and Ganymede on one hand and the rapidly rotating magnetospheric plasma on the other hand. Out of the three, the Io footprint is the brightest and the most studied. Present in each hemisphere, it is made of at least three different spots and an extended trailing tail. The variability of the brightness of the spots as well as their relative location has been tentatively explained with a combination of Alfvén waves’ partial reflections on density gradients and bi-directional electron acceleration at high latitude. Should this scenario be correct, then the other footprints should also show the same behavior. Here we show that all footprints are, at least occasionally, made of several spots and they all display a tail. We also show that these spots share many characteristics with those of the Io footprint (i.e. some significant variability on timescales of 2-3 minutes). Additionally, we present some Monte-Carlo simulations indicating that the tails are also due to Alfvén waves electron acceleration rather than quasi-static electron acceleration. Even if some details still need clarification, these observations strengthen the scenario proposed for the Io footprint and thus indicate that these processes are universal. In addition, we will present some early results from Juno-UVS concerning the location and morphology of the footprints during the first low-altitude observations of the polar aurorae. These observations, carried out in previously unexplored longitude ranges, should either confirm or contradict our understanding of the footprints. [less ▲]

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See detailQualité des médicaments antipaludéens et caractéristiques des pharmacies des territoires périurbains de Kinshasa
Mavungu Landu, Don Jethro ULg; Liégeois, Sophie; Manzambi Kuwekita, Joseph ULg et al

Poster (2016, December 14)

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le ... [more ▼]

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le contrôle de cette maladie. Cependant en Afrique centrale, plus de 25% des médicaments serait contrefaits ou de qualité inférieure, situation qui serait encore plus dramatique dans les territoires périurbains. Méthode: Dans le contexte ci-mentionné, une étude préliminaire et prospective a été menée dans la zone de santé de Mont Ngafula 1 située dans les territoires périurbains de la Ville de Kinshasa durant la période allant du 22 février au 17 mars 2016. Treize échantillons de poudre pour suspension d’artéméther et de luméfantrine ont été collectés. L’analyse de la qualité de ces médicaments a été réalisée au moyen de méthodes séparatives génériques utilisant la technique de chromatographie liquide à haute performance couplé à un détecteur à barrettes de diodes. Une caractérisation a été également effectuée dans 127 établissements pharmaceutiques sur base des normes édictées par le Ministère de la Santé Publique congolais. Résultats: Les résultats des analyses des échantillons d’antimalariques (ou antipaludéens) montre que presque la moitié des poudres pour suspension d’artéméther et de luméfantrine ne contenait pas la concentration prévue en artéméther et/ou en luméfantrine. Par ailleurs, le résultat des observations évoque qu’aucun établissement pharmaceutique ne respecte l’ensemble des normes du Ministère de la Santé Publique. [less ▲]

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See detailRenverser la machine: de la technique comme outil d’exploitation à la technique comme moyen de régénération environnementale: l’approche paysagère comme préalable à la réhabilitation des sites industriels désaffectés dans la Province de Liège.
Baldin, Elisa ULg

Poster (2016, December 12)

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en ... [more ▼]

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en mettant à l’épreuve le principe de « recyclage » architectural et paysager. Le site Espérance Longdoz, à Seraing, représente, en tant qu’objet de mon travail de fin d’études, un cas spécifique mais indicatif d’un phénomène territoriale, qui concerne la Vallée de la Meuse. Le projet d’aménagement urbain, où le re-dessin de l’espace se fonde sur une re-interprétation des traces existantes, ouvre à une réflexion sur la complémentarité des éléments artificiels et des éléments naturels dans le projet de régénération urbaine. L’approche paysagère intégrée est proposée comme outil pour activer des nouveaux cycles de vie, selon une dynamique écologique fondée sur la continuité à trouver entre ressource et déchet: une alternative à l’abandon et à la disparition des lieux qui ont construit l’histoire sociale et l’identité du territoire. Lectures et observations des mutations morphologiques, complétées par des analyses comparatives entre expériences locales et européennes similaires concernant des réalisations, projets en cours et acteurs engagés, fondent une réflexion critique centrée sur le renversement du sens de la machine: autrefois celle qui a exploité et bouleversé les équilibres de la terre et aujourd’hui à l’opposé, celle qui se développe et transforme les milieux à travers temporalités et actions naturelles. La thèse explore les technologies soft, de phyto-dépollution ou autre procédés de transformation naturelle, à réinsérer dans le processus de mutation de nos milieux de vie comme un système de structures d’assainissement environnemental et comme un ensemble de stratégies de reconstitution d’un paysage productif urbain/périurbain, capables de réactiver des moyens soutenables aptes à « renverser la machine » d’exploitation industrielle. [less ▲]

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See detailSolar electrons observed in the magnetosphere
Shi, Quanqi; Wang, linghua; Zong, Qiugang et al

Poster (2016, December 12)

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See detailFACILITER L'ADHÉRENCE AU TRAITEMENT IMMUNOSUPPRESSEUR CHEZ LES TRANSPLANTÉS DU REIN- TEST D'UN SYSTEME DE COACHING PAR SMARTPHONE
Saint-Remy, Annie ULg; Spiroux, Marie; WEEKERS, Laurent ULg et al

Poster (2016, December 08)

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM ... [more ▼]

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM. Krzesinski Introduction: Nonadherence to immunosuppressants is a determining cause of graft loss. The present study tested during 1 month, the usefulness and the effectiveness of a coaching system using smartphone application (the Transplant Smartcoach®) on adherence in kidney transplant recipients. Methodology: The sample included 51 patients (28m/23w) transplanted for 1 year at least, mean age 52±12 years, mean graft survival 3.7±1 years. Each patient had a smartphone configured with its detailed treatment. Using the smartphone application, patient had to notify daily the intake of each tablet into a 2-hour window. If no notification was performed 1 hour later than the scheduled time of intake, the patient was contacted by a nurse to remind him to take medications. Results: 90 % of patients were treated with Tacrolimus and 10 % with cyclosporine, associated with mycophenolic acid (41 %) or mycophenolate mofetil (49 %), 41 % had corticosteroids. Whatever was the immunosuppressant, a perfect adherence (medications taken at the scheduled time) was observed on average in 53 % of the morning monthly intakes. There was no difference in adherence rates between the morning intakes of Advagraf ® (once/day) and Prograft® (2 times/day), a decrease of perfect adherence was identified with the evening intake of Prograft® (53 % vs 44%; P=0.07) and the one of Myfortic® (P=0.03) with consequently an increased frequency of nurse’s recalls. Adherence was lower in younger patients and in those still working. The complexity of treatment (many drugs intake/day) and the respect for time intervals between drug intakes were the major barriers to adherence. When compared to the one measured before using the Smartcoach, the variability (coefficient of variation, %) of the Tacrolimus trough level decreased by 32.6 % (P=0.027) in the 3 to 6 months following the test. Conclusion: patients appreciated the ease and usefulness of the coaching system with smartphone application to help medication adherence. Coupled with therapeutic education of the patients, that tool deserves to be used notably in newly transplanted patients and when a worrying decrease in adherence is observed to help them in the management of a rigorous adherence which should contribute to graft survival. [less ▲]

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See detailXenogeneic graft-versus-host disease: Impact of Th17 cells.
Delens, Loïc ULg; SERVAIS, Sophie ULg; Vrancken, Louise et al

Poster (2016, December 08)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULg; Mendoza Gallego, Carlos ULg; Lismont, Marjorie ULg et al

Poster (2016, December 08)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 2 min while the same reaction in batch took 2h. [less ▲]

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See detailImplication of Importin-8 in mouse brain development
Nganou, Gerry ULg; Coumans, Bernard ULg; De Nijs, Laurence et al

Poster (2016, December 06)

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles ... [more ▼]

Importin-8 (IPO8) is a protein that regulates the nucleocytoplasmic transport of some proteins important for cerebral development (Ago2, c-Jun, Smad4). In HEK cells, IPO8 seems to not plays others roles (like mitotic spindle formation, primary cilium transport). In situ hybridization performed in mouse brains slices shows that IPO8 is already expressed at E14. Moreover, inhibition of its action (IPO8) in embryonic mouse brain leads to impairment of neuroblast migration to upper cortical layers. IPO8 could then be at the origin of some pathologies with neuronal migration deficit. [less ▲]

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See detailOviposition deterring effect of Ocimum basilicum L. (Lamiaceae) on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)
Yarou, Boni Barthélémy ULg; Bawin, Thomas ULg; Boullis, Antoine ULg et al

Poster (2016, December 02)

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic ... [more ▼]

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and producers’ health, there is a need for alternatives. In this study, we assess the oviposition deterring effect of O. basilicum using dual-choice behavioral assays performed in flight tunnels. Two modalities were tested, i.e. (1) a tomato plant associated either with an O. basilicum plant versus a tomato plant associated with a plastic pot only filled with potting soil, and (2) a tomato plant with basil essential oil (EO) formulated in paraffin oil (PO) versus a tomato plant with PO only. A 1 ml cylindrical polyethylene plug loaded with 100 µl of solution (formulated EO or PO) was placed on each plant as a diffuser. Forty-eight hours after the release of unsexed adult individuals randomly sampled from the rearing in the central area of the tunnel, we found that plants and EOs reduced T. absoluta oviposition behavior on a nearby located tomato plant. GC-MS analyses showed that the major constituents include estragol (73.8%), linalool (8.6%), β-elemene (2.9%) in O. basilicum essential oils and E-α-bergamotene (38.9%), methyl eugenol (26.1 %), E-β-ocimene (17.7 % ) in O. basilicum VOCs collected with solid-phase micro-extraction method. These results suggest a valuable potential of O. basilicum and associated essential oils as component of an integrated management strategy against the tomato leafminer. [less ▲]

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See detailGeneralized Pascal triangles and binomial coefficients of words
Stipulanti, Manon ULg

Poster (2016, December 01)

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word ... [more ▼]

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word. Similarly to the Sierpinski gasket that can be built as the limit set, for the Hausdorff distance, of a convergent sequence of normalized compact blocks extracted from Pascal triangle modulo 2, we describe and study the first properties of the subset of [0, 1] × [0, 1] associated with this extended Pascal triangle modulo a prime p. From the extended Pascal triangle obtained when p is equal to 2, we derive a sequence of which we study the regularity and the asymptotic behavior of the summatory function. Inspired from this regularity, we extend our results to another famous numeration systems, namely the Zeckendorff numeration system. [less ▲]

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See detailLe profil en fibres alimentaires de légume modifie leur fermentation intestinale et la production d’acides gras à chaine courte dans modèle in vitro humain
Kalala Bolokango, Gaetan ULg; Kambashi Mutiaka, Bienvenu ULg; Njeumen, Patrick et al

Poster (2016, December 01)

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des ... [more ▼]

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des changements dépendent du type de fibres.Les légumes sont des sources privilégiées de DF, certaines espèces et variétés fournissant davantage de DF solubles (SDF), d’autres étant plus riches en DF insolubles (IDF). Contrairement aux SDF, les IDF sont souvent considérées comme peu fermentescibles, ne contribuant que faiblement à une modulation de l’écophysiologie intestinale. En opposition à cette hypothèse, pour explorer si l’impact potentiel sur la santé intestinale des légumes varie suivant leur nature, des légumes présentant des teneurs et des profils en DF différents ont été comparés dans un modèle in vitro de fermentation colique. [less ▲]

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See detailContinuous Monitoring of Jupiter's Aurora and Io Plasma Torus with the Hisaki Satellite during Joint Observing Campaign with Juno
Kimura, Tomoki; Yamazaki, Atsushi; Murakami, Go et al

Poster (2016, December)

The Hisaki satellite is the first space telescope that is dedicated to observations of our solar system bodies. Hisaki has been continuously monitoring the atmosphere and magnetosphere of the solar system ... [more ▼]

The Hisaki satellite is the first space telescope that is dedicated to observations of our solar system bodies. Hisaki has been continuously monitoring the atmosphere and magnetosphere of the solar system bodies with the extreme ultraviolet (EUV) spectroscope EXCEED since the launch in September 2013. Dynamics on timescales from 10s minutes to a few years were discovered in the atmosphere and magnetosphere by the continuous monitoring. Large joint observing campaigns for Jupiter by the Juno spacecraft in coordination with Hisaki, the Hubble Space Telescope (HST), and other facilities started in mid-2016. Before Jupiter Orbit Insertion, Juno made the in-situ solar wind measurements while Hisaki was simultaneously monitoring Jupiter's aurora and Io plasma torus. On Day of Year 142, Hisaki discovered that the EUV auroral emission power rapidly increased by a factor of ~3 but returned to a nominal level within one planetary rotation. The transient aurora was followed by brightening of the torus sulfur and oxygen ions that is proxy of the hot electron populations in the torus. At the same time, the relatively weak solar wind shock signatures were found in the JUNO/JEDI energetic particle data (Clark et al.). The transient variability is compared to the morphology observed by HST (Nichols et al.). Hisaki is going to monitor Jupiter in collaboration with the large observing program of HST (Grodent et al.) that will make ~150 exposures from this November. [less ▲]

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See detailMultianalytical Study of “Le Sorcier d’hiva Oa” by Gauguin
Defeyt, Catherine ULg; Remon, Regine; Strivay, David ULg

Poster (2016, December)

Although there is abundant literature on the worldwide famous French painter, Paul Gauguin, very little is known concerning his painting techniques and materials. The present research aims to throw a new ... [more ▼]

Although there is abundant literature on the worldwide famous French painter, Paul Gauguin, very little is known concerning his painting techniques and materials. The present research aims to throw a new light on Gauguin’s œuvre through the in depth study of Le Sorcier d’ Hiva Oa, painted by Gauguin during his stay in French Polynesia in 1902, by means of non-invasive imaging and analytical techniques. While the infrared reflectography examination of the painting revealed how the artist achieved his final composition, the results obtained by Raman spectroscopy and X ray fluorescence spectroscopy in punctual and mapping modes allowed identifying the different pigments he combined to obtain the bright color shades characterizing this artwork. [less ▲]

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See detailProperties of Jupiter’s auroral acceleration region inferred with HST-STIS spectral images
Ray, Licia C.; Arridge, Christopher S.; Gustin, Jacques et al

Poster (2016, December)

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. Precipitating auroral electrons are part of a current system which transports angular momentum from the planetary ... [more ▼]

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. Precipitating auroral electrons are part of a current system which transports angular momentum from the planetary atmosphere to sub-corotating magnetospheric plasma. The magnitude of the currents and hence precipitating energy flux, are sensitive to the characteristics of the high-latitude magnetosphere, in particular the location of the auroral acceleration region (AAR) and the density and temperature of the high-latitude electron population. We use HST STIS observations of Jupiter’s aurora (Gustin et al. [2016]) to infer the location of the AAR and the properties of the precipitating auroral electrons. To do this, we determine the energy of the precipitating electrons and incident energy flux for the two distinct regions within the main aurora and within flare regions. The resulting relationships between energy flux and electron precipitation energy for the main auroral emission are then compared to the theoretical relationship derived by Lundin & Sandahl [1978], in order to derive the location of the AAR and the temperatures and densities of the electrons at the top of the AAR prior to acceleration. We find that that each emission region is best reproduced using a multiple auroral acceleration regions with different properties, rather than a single auroral acceleration region with a varying potential drop strength. [less ▲]

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See detailUn hydrogel de chitosan d’origine non-animale restaure la fonction rhéologique du fluide synovial arthrosique : étude ex vivo
Henrotin, Yves ULg; Rocasalbas, Guillem; Chausson, Mickaël et al

Poster (2016, December)

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See detailImportin-8 mutations could cause Jeavons Syndrome, CAE and JME by altering early neuroblast migration
Nganou, Gerry ULg; Tanaka, Miyabi; Coumans, Bernard ULg et al

Poster (2016, December)

Jeavons syndrome (JS) is an uncommon form of juvenile epilepsy. In some family affected by JS, the gene of importin-8 (IPO8) a member of karyopherin superfamily of proteins, has been found mutated ... [more ▼]

Jeavons syndrome (JS) is an uncommon form of juvenile epilepsy. In some family affected by JS, the gene of importin-8 (IPO8) a member of karyopherin superfamily of proteins, has been found mutated. Karyopherin are known to regulate nucleo-cytoplasmic transport of many proteins. IPO8, a member of the β-karyopherin sub-family, is reported to control the transport Ago-2, c-Jun and Smad-4 for example, three proteins important for brain development. Here, we show that IPO8 is well expressed in mouse brain at embryonic stage. Moreover inhibition of IPO8 mRNA by shRNA in-utéro-electroporation (IUE), impairs early neuroblast migration as weel as IUE overexpression of mutated form of human IPO8. So, abnormal brain development due to IPO8 mutations could be at the origin of Jeavons Syndrome, Chilhood abscence evolving (CAE) and/or juvenile myoclonic epilepsy (JME). [less ▲]

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See detailEtude de l’évolution de la pathologie arthrosique et des propriétés mécaniques du cartilage sur un modèle d’arthrose spontanée chez le cobaye Dunkin-Hartley
Legrand, Catherine ULg

Poster (2016, December)

Dans les modèles animaux, la gravité des lésions du cartilage est appréciée grâce à des scores histologiques évaluant la structure, le contenu en protéoglycane, l’intégrité de la tidemark, la cellularité ... [more ▼]

Dans les modèles animaux, la gravité des lésions du cartilage est appréciée grâce à des scores histologiques évaluant la structure, le contenu en protéoglycane, l’intégrité de la tidemark, la cellularité, ainsi que les ostéophytes. Parallèlement à ces analyses histologiques, nous avons étudié les propriétés mécaniques du cartilage à différents stades d’évolution de la maladie chez le cobaye Dunkin-Hartley. Nous avons également corrélé la sévérité des lésions histologiques avec les propriétés mécaniques du cartilage. [less ▲]

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See detailFirst Hubble Space Telescope Movies of Jupiter’s Ultraviolet Aurora During the NASA Juno Prime Mission
Grodent, Denis ULg; Gladstone, G. Randall; Clarke, John T. et al

Poster (2016, December)

The primary goal of this HST campaign is to complement Juno-UVS (Ultraviolet Spectrograph) observations. This complementarity is four-fold as HST observes Jupiter’s aurora when: 1) Juno-UVS is turned off ... [more ▼]

The primary goal of this HST campaign is to complement Juno-UVS (Ultraviolet Spectrograph) observations. This complementarity is four-fold as HST observes Jupiter’s aurora when: 1) Juno-UVS is turned off, that is about 98% of Juno’s 14-day orbit, and Juno’s in situ instruments are in operation. 2) Juno-UVS is operating, but observes the opposite hemisphere of Jupiter. 3) UVS is on in the same hemisphere, but too close to Jupiter to have a global, contextual, view of the aurora and/or UVS is affected by the noise induced by Jupiter’s radiation belts. 4) Juno is too far from Jupiter to get a detailed view of the aurora. In addition, HST will observe the auroral and airglow emissions of the Galilean moons Io, Ganymede and Europa, when UVS is measuring their auroral footprints in Jupiter’s ionosphere. During this campaign, HST is obtaining 45-min STIS time-tag images -movies- of both hemispheres of Jupiter and STIS/COS spectra of Jupiter's moons. These observations are taking place during 4 sequences of Juno's orbit (Figure: typical orbit in magnetic coordinates): 1) Perijove segment: a 6-hour sequence bracketing the time of Juno's closest approach of Jupiter. 2) Crossing segments: few hours periods during which Juno is crossing the magnetic equator of Jupiter and in situ instruments are observing the plasma sheet particles. 3) Perijove +/- 1 Jovian rotation (or more), to provide a context for the auroral activity before and after perijove. 4) Apojove segment: a 12-hour period bracketing the time when Juno is farthest from Jupiter and Juno-UVS is continuously monitoring the global auroral UV power of Jupiter. During Juno orbit PJ5, between 28 Nov. and 07 Dec. 2016, HST obtains 9 STIS movies: 3 movies of the northern aurora near perijove, 1 movie (north) one Jovian rotation before and 2 movies (south- north) one and two Jovian rotations after perijove, 2 movies (north) during two close CS crossings, and 1 movie near apojove. These movies will be commented during this presentation. [less ▲]

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See detailComment mesurer correctement la PA chez les patients hémodialysés?
VANDERWECKENE, Pauline ULg; SAINT-REMY, Annie ULg; KRZESINSKI, Jean-Marie ULg

Poster (2016, December)

Objectifs : Le contrôle de la pression artérielle (PA) chez le patient hémodialysé (HD) est un enjeu majeur en raison des résultats controversés sur les risques cardiovasculaires qui y sont associés ... [more ▼]

Objectifs : Le contrôle de la pression artérielle (PA) chez le patient hémodialysé (HD) est un enjeu majeur en raison des résultats controversés sur les risques cardiovasculaires qui y sont associés. Cependant, la mesure de la PA dans cette population est difficile à appréhender au vu de sa grande variabilité. L’exactitude des mesures péridialytiques (enregistrées dans l’unité d’HD pendant la séance) est souvent remise en question. L’objectif de notre étude est d’établir la contribution et la concordance entre deux techniques de mesure ambulatoire pour détecter la PA non contrôlée chez ces patients HD. Il s’agit du monitoring ambulatoire interdialytique de 44h (MAPA) et de l’automesure au domicile (HBPM) pendant une durée variable de 3, 5 et 7 jours. Méthodologie : 43 patients hémodialysés chroniques (H=28, F = 15), avec une moyenne de 68,3±13 ans ont réalisé une MAPA de 44h (Spacelabs 90207), de la fin d’une séance d’HD au début de la suivante, immédiatement suivie par une automesure de 7 jours (Omron M6). Les mesures péridialytiques étaient la moyenne des PA pré-dialyse ou post-dialyse enregistrées sur 2 semaines (6 séances d’HD). L’hypertension était définie par une PA égale ou supérieure à 140/90 mmHg pour la PA pré-HD, 130/80 mmHg pour la PA post-HD, 130/80 mmHg pour la MAPA de 44 h et 135/85 mmHg pour l’HBPM. Résultats : Nous avons constaté de bonnes corrélations entre la MAPA et l’HBPM pour le diagnostic de l’hypertension chez le patient HD. Comparées aux PA péridialytiques, les 2 techniques ambulatoires ont permis d’identifier la même proportion d’hypertension masquée et d’hypertension de la blouse blanche (25%). La plus grande précision de diagnostic sur le status tensionnel est retrouvée pour les plus longues périodes d’enregistrement (44h pour la MAPA et 7 jours pour l’HBPM). Les plus courtes périodes étaient mieux tolérées par les patients mais moins précises pour détecter l’hypertension. L’automesure était plus appréciée que la MAPA de 44h. Conclusion : La MAPA et l’automesure ont permis d’identifier un quart de patients avec un phénotype particulier d’hypertension, qui n’aurait pas été classé correctement en se fiant uniquement aux mesures péridialytiques. L’automesure devrait être recommandée en première ligne chez le patient HD (au moins une semaine/mois). Cependant, la MAPA fournit des informations uniques comme la PA nocturne et pourrait être proposée, idéalement une fois par an, chez tous les patients. [less ▲]

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See detailDevelopment and comparison of liposomes and nanocapsules as injectable nanocarriers for poorly aqueous soluble drugs
Karim, Reatul ULg; Palazzo, Claudio ULg; Laloy, Julie et al

Poster (2016, December)

About 90% of drugs in development phase have poor aqueous solubility. Liposomes and nanocapsules are promising approaches that enable parenteral administration of these drugs with possibilities of site ... [more ▼]

About 90% of drugs in development phase have poor aqueous solubility. Liposomes and nanocapsules are promising approaches that enable parenteral administration of these drugs with possibilities of site specific delivery. The objective of the study was to develop different liposomes and lipid nanocapsules entrapping a hydrophobic model molecule (apigenin (AG)), and to characterize and compare them as potential injectable nanocarriers (NCs) for drugs with low aqueous solubility. [less ▲]

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See detailNEW INJECTABLE LIPOSOME AND DRUG-IN-CYCLODEXTRIN-IN LIPOSOME SYSTEMS ENCAPSULATING ESTETROL FOR THE TREATMENT OF ISCHEMIA DISEASES IN PREMATURE BABIES
Palazzo, Claudio ULg; Laloy, Julie; Delvigne, Anne-Sophie et al

Poster (2016, December)

Purpose: In 2010, almost 15 million of babies in the world are prematurely borned, 11.1 % of the total amount of alive children. Despite the better neonatology techniques, the number of preterm babies ... [more ▼]

Purpose: In 2010, almost 15 million of babies in the world are prematurely borned, 11.1 % of the total amount of alive children. Despite the better neonatology techniques, the number of preterm babies with motor, vision, hearing or mental deficiencies is still constant along the last twenty years. Moreover, no efficacy treatment is available to the present day. The estetrol (E4) has an important role in the brain development and protection. The aim of this study is to develop new injectable liposome and drug-in-cyclodextrin-in-liposome (DCL) formulations, encapsulating E4 in order to enhance its crossing through the blood-brain barrier (BBB). Methods: Cyclodextrins (CD) were used to increase E4 aqueous solubility. Liposome and DCL (E4-CD complex) formulations were prepared by thin-film hydration technique. The formulations were physicochemical characterized. LDH and MTS tests on endothelial, neuronal and BBB model cells were performed in vitro on the liposome formulation. Hemocompatibility of the formulations was evaluated on red blood cells, platelet aggregation and coagulation. BBB passage tests were performed using human BBB cell line (hCMEC/D3). Results: E4-CD complexes proportionally increased the solubility of the hormone. Liposomes and DCL encapsulating E4 were prepared. All the formulations had average particle size below 150 nm, polydispersity index below 0.10 and ζ potential around + 30 mV. The encapsulation efficacy for liposomes was between 3% and 10% while those of DCL are between 15% and 35%. Moreover, the formulations are capable to release 80 % (liposome) and 90 % (DCL) of encapsulated E4 after 3 h at 37°C. The effect of liposome and DCL formulations on cell viability and integrity was evaluated. The results showed no toxic effects on all the tested cell lines. Hemocompatibility tests showed no hemolysis, platelet aggregation or effects on coagulation, confirming the possibility of the formulations to be intravenously administrated. BBB passage tests highlighted the capability of the formulations to pass the BBB and reach the brain. Conclusions: New non-toxic, hemocompatible liposome and DCL formulations encapsulating E4 were prepared. The formulations are promising drug delivery system to target estrogens to the brain, due to their physiochemical characteristics. [less ▲]

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See detailIntramolecular continuous-flow strategy towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULg; Winter, Marc; Vizza, Alessandra et al

Poster (2016, December)

threo-Methylphenidate hydrochloride is the most widely prescribed drug to treat attention deficit hyperactivity disorder (ADHD). Herein we present the batch-to-flow translation of a carbene-based ... [more ▼]

threo-Methylphenidate hydrochloride is the most widely prescribed drug to treat attention deficit hyperactivity disorder (ADHD). Herein we present the batch-to-flow translation of a carbene-based intramolecular strategy for the diastereoselective preparation of this active pharmaceutical ingredient. The process relies on the thermal generation and decomposition of a hazardous diazospecies, its thermal intramolecular C-H insertion reaction yielding an intermediate β-lactam, and its subsequent acidic methanolysis, giving enriched threo-methylphenidate hydrochloride in 70% isolated yield. The automated microfluidic setup telescopes 2 chemical transformations, 1 intermediate in-line purification and sustains a daily productivity of 20.4 g/day of methylphenidate hydrochloride. The preparation of the β-lactam is implemented on mesoscale in a pilot-scale continuous-flow reactor, providing 4.25 kg of this key intermediate per day. [less ▲]

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See detailMobility of 137Cs, 10Be and 210Pb in soil as a function of the soil texture, organic matter content and pH
de Tombeur, Félix ULg; Cornu, Sophie; Bourlès, Didier et al

Poster (2016, November 30)

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ... [more ▼]

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ecosystem services. In order to better constrain and quantify these processes, a kinetic quantification of the involved transfers based on vertical profiles of 210Pb (xs), 10Be and 137Cs has been developed for Luvisols in which soluble transfers could be neglected. The range of applicability of this type of approach was nevertheless not tested. In this work, we analyzed 210Pb (xs), 10Be and 137Cs in soils exhibiting gradients of organic matter, pH and < 2 μm fraction (Podzol, Andosol Ferralsol, Leptosol). Acidic soils and large organic matter contents were considered. The depth distribution of the studied isotopes was then interpreted as a function of the organic matter content, the soil pH and its < 2 μm fraction. This demonstrates that 10Be and 137Cs losses occur under soluble form in the Podzol, especially under forest, more acid, for the latter. Soluble 10Be transfers were also identified in the Andosol when they were considered as negligible in the Leptosol. For 210Pb (xs), soluble transfers are also probable for soils with pH less than 5.5 (Andosol and Ferralsol) and strong affinity for organic matter has been demonstrated. Thus, under acidic conditions and low < 2 μm fraction, soluble transfer cannot neglected anymore. [less ▲]

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See detailForce-Velocity-Power Profiles in Elite Young Soccer Players
Miny, Kevin ULg; Jidovtseff, Boris ULg

Poster (2016, November 30)

The main purpose of this research was to assess the isoinertial Force-Velocity-Power (F-V-P) profile in young elite soccer players. Furthermore, this research aims to compare force, velocity and power ... [more ▼]

The main purpose of this research was to assess the isoinertial Force-Velocity-Power (F-V-P) profile in young elite soccer players. Furthermore, this research aims to compare force, velocity and power qualities among different age categories (under (U) 17, U19 & U21). The ultimate objective is to better understand the relationships between neuromuscular system and functional performance. [less ▲]

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See detailThe influence of specific training on the plyometric profile
Jidovtseff, Boris ULg; Harris, Nigel; Carpent, Nicolas et al

Poster (2016, November 30)

Sprinting, jumping and change of direction involve the stretch-shorten cycle (SSC) and therefore plyometric training is used to improve these factors. It has been demonstrated that the jumping strategy ... [more ▼]

Sprinting, jumping and change of direction involve the stretch-shorten cycle (SSC) and therefore plyometric training is used to improve these factors. It has been demonstrated that the jumping strategy utilized can influence the biomechanical profile of the exercise and subsequent adaptation. For example, in some cases, it is important to jump as high as possible while in other cases it is important to reduce the ground contact time. Our hypothesis is that the choice of plyometric exercise has to be matched to the desired biomechanical adaptation. Hence we investigated the influence of 8 weeks of different programs on the plyometric profile of recreational athletes. In each of five groups, very specific exercises were selected according to the training objective: ground contact time group (CT, n=9); vertical jump height group (JH, n=9); CT and JH combination group (CT-JH, n=11); JH + strength training group (JH-S, n=9); and control group (CO, n=8). The plyometric profile performed prior to and post training included measures of jump height, contact time, stiffness and reactivity at different bounding intensities. The results demonstrated that JH and JH-S programs were more effective for improving jump height performance (+7-9%; p<0.005) compared to insignificant jump height changes in the CT and CO groups. CT-JH was the most effective on the reactivity index (+14%, p<0.005) although significant increases (+8%, p<0.05) were also observed in CT, JH and JH-S groups. CT was the only group to significantly decrease short contact time (-5%, p<0.05). ANOVA analysis revealed significant groups*session effect for jump height (p<0.01) and reactivity (p<0.005) but not for short contact time. The present study confirms that the principle of specificity is fundamental in plyometric training so the exercise selection should be developed cognizant of intended adaptations. Jump height and reactivity appear to be differentially affected by specific training practices. [less ▲]

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See detailInvolvement of Ikbζ in glioblastomas and its potential implication in radioresistance
Dubois, Nadège ULg; Willems, Marie; Kroonen, Jérôme et al

Poster (2016, November 29)

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See detailViral glycoprotein gp150 promotes sexual transmission of Murid Herpesvirus-4
Zeippen, Caroline ULg; Javaux, Justine ULg; Xiao, Xue ULg et al

Poster (2016, November 28)

Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of ... [more ▼]

Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of immune response. Understanding these strategies is likely to be important to control infection. Interestingly, we recently observed that Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus infecting laboratory mice, could be sexually transmitted between mice. This model offers therefore the opportunity to understand the mechanisms underlying natural transmission. Some of these mechanisms could rely on the glycoprotein 150 (gp150), which could limit virus neutralization and promote the release of infectious particles from cells. In this study, we tested therefore the importance of gp150 in the context of MuHV-4 sexual transmission. Briefly, female mice were infected with WT or gp150- strains expressing luciferase. They were imaged with an in vivo imaging system to follow infection. When lytic replication was observed in the genital tract, infected females were mated with naïve males to compare the capacity of transmission of the two strains. Our results show that, while the gp150- strain has no deficit in reaching and replicating in the female genital tract, it displays a major deficit of sexual transmission in comparison with WT virions. Interestingly, this deficit appears to reflect a deficit of virions release from vaginal epithelial cells. Altogether, our results show that, while gp150 is not required for efficient dissemination and maintenance of MuHV-4 within its host, it is essential for efficient transmission, by promoting the releasing of infectious particles from the mucosal cells. [less ▲]

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See detailL'activation comportementale est-elle pertinente pour tous les adultes dépressifs ? Une analyse en clusters
Wagener, Aurélie ULg; Baeyens, Céline; Van der Linden, Martial ULg et al

Poster (2016, November 26)

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression ... [more ▼]

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression soulignent l’importance de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes dans le maintien de la dépression. Cette étude poursuit deux objectifs : (1) investiguer l’hétérogénéité de la depression via une analyse en clusters, (2) investiguer les valeurs prédictives de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes sur la dépression en fonction du cluster d’appartenance. METHODE : Des adultes tout venant et/ou consultant des centres de santé mentale ont été recrutés (âge entre 18 et 60 ans). Ils ont complété l’Inventaire de Dépression de Beck – Seconde Edition, l’Echelle d’Activation Comportementale pour la Dépression – Version courte, et l’Inventaire des événements plaisants. RESULTATS : Une solution en six clusters a été mise en évidence : “gros dormeurs/heavy sleepers”, “dépressifs cognitifs/cognitive depressives”, “dépressifs affectifs-somatiques/affective-somatic depressives”, “dépressifs légers/mild depressives”, “dépressifs dormant peu/sleepless depressives”, “dépressifs typiques/typical depressives”. Des relations significatives entre inactivité, fréquence d’activités plaisantes et dépression ont seulement été observées chez les “dépressifs cognitifs/cognitive depressives”, les “dépressifs légers/mild depressives” et les “dépressifs typiques/typical depressives”. DISCUSSION : Les résultats confirment l’hétérogénéité de la dépression puisque six clusters ont été mis en évidence. Par ailleurs, l’activation comportementale ne semble pertinente que pour trois de ces clusters dans lesquels les postulats du modèle comportemental de la dépression sont confirmés (“dépressifs cognitifs/cognitive depressives”, “dépressifs légers/mild depressives”, “dépressifs typiques/typical depressives”). [less ▲]

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See detailLa Perspective Temporelle Equilibrée: Validation d’un questionnaire et relation avec la régulation émotionnelle.
Barsics, Catherine ULg; Rebetez, Marie My Lien; Rochat, Lucien et al

Poster (2016, November 26)

La Perspective Temporelle Equilibrée fait référence à une disposition individuelle caractérisée par le fait de se projeter mentalement dans le futur et dans le passé de manière positive et fréquente ... [more ▼]

La Perspective Temporelle Equilibrée fait référence à une disposition individuelle caractérisée par le fait de se projeter mentalement dans le futur et dans le passé de manière positive et fréquente. L’objectif de notre étude était de valider la version française d’une échelle, initialement conçue en anglais, évaluant cette orientation temporelle : la “Balanced Time Perspective Scale” (Webster, 2011). Une étude en ligne a permis de recueillir les données de 622 participants francophones issus de la population générale. Les résultats indiquent que la version française réplique la structure en deux facteurs du questionnaire original et plaident en faveur d’une bonne validité de construit. De surcroît, les résultats montrent qu’une tendance importante à se projeter mentalement dans le futur et dans le passé de manière positive et fréquente est associée à la réévaluation cognitive, une stratégie de régulation émotionnelle. En somme, ce questionnaire permet d’évaluer la perspective temporelle, qui est ici envisagée en tant qu’importante ressource pour le self, et ce, en étroite relation avec les capacités de régulation émotionnelle. [less ▲]

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See detailEducation, Complexity of Work, and Incident Cognitive Impairment in Puerto Rican Older Adults
Andel, Ross; Luisa, Ana; Small, Brent et al

Poster (2016, November 18)

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See detailLimited impact of imatinib in a murine model of sclerodermic chronic graft-versus-host disease
Belle, Ludovic; Fransolet, Gilles ULg; SOMJA, Joan ULg et al

Poster (2016, November 17)

Background: Graft-versus-host disease (GVHD) remains one of the major complications following haematopoietic stem cell transplantation (HSCT). Approximately 15% of the patients with chronic GVHD develop ... [more ▼]

Background: Graft-versus-host disease (GVHD) remains one of the major complications following haematopoietic stem cell transplantation (HSCT). Approximately 15% of the patients with chronic GVHD develop the sclerodermatous form of the disease characterized by multiple organ fibrosis and loss of skin elasticity. Several studies have shown the potential benefits of imatinib, a tyrosine kinase inhibitor, as a treatment of fibrosis in cGVHD due to its ability to inhibit simultaneously PDGF-R and c-Abl pathways which are both involved in fibrosis mechanisms. This work investigates the possible benefits of imatinib on fibrosis in a murine model of sclerodermatous chronic GVHD (sclcGVHD). Methods: Lethally irradiated Balb/cJ mice (7 Gy TBI) were injected i.v. with 10.106 bone marrow cells and 70.106 splenocytes from B10.D2 donnor mice. Mice were then treated with sterile water or imatinib (150 mg/kg/day) by oral gavage from day +7 to day +52 following transplantation. GVHD severity was assessed three times/week with a scoring system encompassing 5 criteria (mice posture, weight loss, activity, hair loss, skin integrity ; 0-1-2 point(s)/criteria). Results: Our results show that imatinib failed to prevent/improve GVHD with a similar evolution of the GVHD severity with no differences between groups. Histological analyses indicate a significant reduction of the phosphorylation level of the PDGR receptor (p = 0,033) and a trend to a decreased level of phosphorylated c-Abl (p = 0,1854). In vivo cell proliferation assay with CFSE were also performed and showed a reduced proliferation of T cells and subsets (CD4, CD8 and Tregs) in spleen, lymph nodes, bone marrow and blood after imatinib treatment. Finally, FACS analyses performed on days +21 and +35 after transplantation did not show any differences in the absolute T-cell counts. Conclusion: Although we have observed a decreased phosphorylation level of PDGR receptor and less proliferation of T cells and subsets in vivo, imatinib failed to alleviate scl-cGVHD in a murine model of severe scl-cGVHD. [less ▲]

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See detailHot-Melt Extrusion as a Continuous Manufacturing Process to Form Ternary Cyclodextrin Inclusion Complexes
Thiry, Justine ULg; Krier, Fabrice; Ratwatte, Shenelka et al

Poster (2016, November 16)

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See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULg; Centonze, Prescilia ULg; Comblain, Fanny ULg et al

Poster (2016, November 16)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

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See detailZinc, iron and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2016, November 16)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). [less ▲]

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See detailPolyphenol content and antioxidant capacity in various commercial juices
Matute Matute, Alexis Fernando ULg; Tabart, Jessica; CHERAMY-BIEN, Jean-Paul ULg et al

Poster (2016, November 16)

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free ... [more ▼]

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free radicals promoting positive effects on human health due to their antioxidant capacity. In this study we evaluated the content of polyphenols from several commercial biological (n = 6) and conventional juices (n = 6 ) (orange, blackcurrant, apple, grapes, tomato, carrot, pomegranate, lemon, grapefruit juices) and we determined the antioxidant effect by inhibition of the superoxide anion production measured by chemiluminescence technology. Conventional juices (orange, apple, tomato, pomegranate, lemon, grapefruit juices) showed more total polyphenolics (+ 32%), more flavan-3-ols (+ 67%) and more flavonols (+ 42%) than the same biological juices. The presence of anthocyanins was reported in juices made with blackcurrant, pomegranate and grapes only, with values above 50 µg/mL, regardless of their nature. Surprisingly, chemiluminescence results showed an inhibition in superoxide anion production by 51% for biological juices vs 23% in conventional ones. The correlation between total polyphenol content and antioxidant capacity of all the juices was r = 0.83 (r2 = 0.7). The fact that there is less polyphenols and higher antioxidant activity in bio juices could be related to the concentration and the type of polyphenol present. [less ▲]

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See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, November 16)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells. We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood. : We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells. [less ▲]

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See detailThe BCCM/ULC collection to conserve the biodiversity and study the secondary metabolites of Polar cyanobacteria
Lara, Yannick ULg; Durieu, Benoit ULg; Renard, Marine ULg et al

Poster (2016, November 16)

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in ... [more ▼]

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts. Their success in these harsh cold conditions can probably be explained by particular adaptations to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other environmental stresses. The BCCM/ULC public collection is funded by the Belgian Science Policy Office since 2011. It has obtained the ISO9001 certification for deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. This collection aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths,….) and make it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, pigments, and genomic make-up. It presently includes 226 cyanobacterial strains, of which 119 are of Antarctic origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). As shown by morphological identification, the strains belong to five orders (Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales). The 16S rRNA and ITS sequences of the strains are being characterized. The first 85 Antarctic strains already studied are distributed into 25 Operational Taxonomic Units (OTUs = groups of sequences with > 97,5% 16S rRNA similarity), and thus, represent a quite large diversity. Moreover, strains identified as members of the genera Leptolyngbya or Phormidium appear in several lineages. This supports the idea that there is a need to revise the taxonomy of these polyphyletic genera with a simple filamentous morphology. To better understand the functioning, metabolism and adaptative strategies of cyanobacteria in the extreme Antarctic environment, the genome sequencing of 11 strains has been started. Pair-read data from illumina MiSeq runs were obtained and submitted to a bioinformatic pipeline dedicated to the assembly of genomes and search of sequences involved in the biosynthesis of secondary metabolites. Gene cluster prediction analysis allowed to characterize 20 clusters of NRPS, PKS and hybrid NRPS-PKS from 2 to 66kb. Surprisingly, none of the characterized operons had previously been described in the literature. [less ▲]

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See detailDevelopment of a new method for arabidopsides extraction and purification
Genva, Manon ULg; Andersson, Mats X; Nasir, Mehmet Nail ULg et al

Poster (2016, November 16)

In the present work, a new method for arabidopsides extraction and purification was developed.

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See detailA crucial role for cdk1 in postnatal hippocampal neurogenesis
Marlier, Quentin ULg

Poster (2016, November 15)

Cdk1 seems to be involved in the proliferation and differentiation of adult neural stem cells found in the adult dentate gyrus

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See detailElektrodynamische Fragmentierung von Elektroschrott
Iseli, Cédric; Streicher-Porte, Martin; Martino, Rémi ULg et al

Poster (2016, November 11)

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden ... [more ▼]

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden kann. Verschweisste Kompaktgeräte können zum Beispiel so weit geöffnet werden, dass die ge-wünschte Komponente freigelegt und entfernt werden kann. Leiterplatten haben die Eigenschaft, dass bei kontinuierlich steigendem Energieeintrag zuerst die aufgebrachten Komponenten abgetrennt werden, bevor die Cu-Bahnen freigelegt werden. Bei noch höherem Energieeintrag wird die Leiterplatte schlussendlich zerkleinert und vollständig zer-stört. Durch diese Entfrachtung der Leiterplatte kann ein metallreiches Konzentrat hergestellt werden. [less ▲]

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See detailEndocrine disruptors and parasitism: which effects on the sex behaviour of the amphipod Gammarus pulex ?
Fivet, Adeline; Horion, Sibylle; Thomé, Jean-Pierre ULg et al

Poster (2016, November 10)

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See detailA Model of Endogenous Insulin Secretion During Exercise
Thomas, Felicity Louise ULg

Poster (2016, November 10)

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