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See detailA salt tracer test monitored with surface ERT to detect preferential flow and transport paths in fractured/karstified limestones
Robert, Tanguy ULg; Caterina, David ULg; Deceuster, John et al

in Berichte der Geologischen Bundesanstalt (2012, September), 93

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See detailPlatelet-rich plasma (PRP) to treat patellar tendinopathy: preliminary results
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; SIMONI, Paolo ULg et al

in Regional Anesthesia & Pain Medicine (2012, September), 37(5 - Suppl 1), 216

Introduction: Patellar tendinopathy, often remain rebel to conservative treatments. Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. The aim of ... [more ▼]

Introduction: Patellar tendinopathy, often remain rebel to conservative treatments. Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. The aim of our study was to investigate the effect of 1 injection of PRP in patients suffering from chronic patellar tendinopathy. Methods: Patients performed imaging (US and MRI) and functional assessments, and a clinical examination using an algometer, before treatment and 6 weeks after PRP treatment. They were also invited to answer to questionnaire relative to pain and functional status. PRP was obtained from autologous blood using an apheresis system (COM.TEC, Fresenius). The injection of 6mL of PRP was realised without local anaesthesia into the proximal insertion of the patellar tendon. A 48h rest-time was recommended after infiltration. Afterwards, a submaximal eccentric reeducation was initiated 1 week after infiltration 3 times a week during 5 weeks. In case of pain, anti-inflammatory drugs were prohibited and patient were encouraged to take class I or II painkillers. Results: At this time, 10 patients with patellar tendinopathy were included in our study. Pre-injection tests revealed pain of the upper part of the patellar tendon just below the patella, associated with loss of function. Imaging exams confirmed diagnosis. Six weeks post-injection, the clinical status was improved in all patients, with a significant decrease of algo-functional scores. The pain reported during functional assessments was decreased (in particular for the eccentric actions), yet no significant improvement of physical performances was observed. We found no significant differences between imaging exams before and 6 weeks after PRP injection. Conclusion: One in situ injection of PRP clinically improved patients with patellar tendinopathy 6 weeks after treatment. All the 10 patients reported a decrease of pain during day-life and through physical activities. However nor functional performances neither imaging were improved. [less ▲]

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See detailAnodal transcranial direct current stimulation over the visual cortex as a preventive treatment of migraine: a proof-of-concept study.
Sasso d'Elia, Tullia; Vigano, Alessandro; SAVA, Simona Liliana ULg et al

in Frontiers in Human Neuroscience (2012, September)

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See detailAires et agrandissements. Maths & Manips pour la transition primaire-secondaire
Guissard, Marie-France; Henry, Valérie ULg; Lambrecht, Pauline et al

in Losanges (2012, September), 18

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See detailImpact of extracorporeal shock wave therapy in the treatment of chronic lateral epicondylitis
Deroanne, Adrien; Deroanne, Didier; Florkin, Marc et al

in Regional Anesthesia & Pain Medicine (2012, September), 37(5 - Suppl 1), 263

Background and aim: radial shock wave therapy (RSWT) is a relatively new way to treat chronic tendinopathies, such as lateral epicondylitis. However, very few studies have been realized on this subject ... [more ▼]

Background and aim: radial shock wave therapy (RSWT) is a relatively new way to treat chronic tendinopathies, such as lateral epicondylitis. However, very few studies have been realized on this subject, and the results are very divergent. We aimed to observe the impact of this technique on chronic lateral elbow pain. Method: fifteen subjects who had a lateral epicondylitis for at least 3 months were included in the study. Two groups were formed: experimental (10 subjects) who received 6 sessions of physiotherapy and RSWT, and the control group (5 subjects) who received exclusively physiotherapy. Physiotherapy sessions were composed of massage, stretching, diacutaneous fibrolysis, and a muscular eccentric program of the wrist extensor muscles. We evaluated the subjects before the first session, and after 6 weeks of treatment with a pain visual analog scale (VAS), the painless wrist flexion amplitude, ant the “Patient-Rated Tennis Elbow Evaluation” (PRTEE) questionnaire. Results: the difference between initial and final evaluations was significant (regarding to the wilcoxon test) for all of the parameters studied (p=0,028 for the VAS, p=0,005 for the wrist flexion amplitude, and p=0,005 for the PRTEE) in the experimental group. It wasn’t significant in the control group (VAS p=0,144128, wrist flexion amplitude p=0,079617, and PRTEE p=0,067890). The comparison between the two groups was not significant, neither in the beginning, nor in the end of the treatment (regarding to the Umann and Whitney test) Conclusions: RSWT associated to physiotherapy is a more effective treatment for lateral epicondylitis than physiotherapy alone. [less ▲]

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See detailComparison of temperature estimates from heat transport model and electrical resistivity tomography during a shallow heat injection and storage experiment
Hermans, Thomas ULg; Daoudi, Moubarak ULg; Vandenbohede, Alexander et al

in Berichte der Geologischen Bundesanstalt (2012, September), 93

Groundwater resources are increasingly used around the world as geothermal systems. Understanding physical processes and quantification of parameters determining heat transport in porous media is ... [more ▼]

Groundwater resources are increasingly used around the world as geothermal systems. Understanding physical processes and quantification of parameters determining heat transport in porous media is therefore important. Geophysical methods may be useful in order to yield additional information with greater coverage than conventional wells. We report a heat transport study during a shallow heat injection and storage field test. Heated water (about 50°C) was injected for 6 days at the rate of 80 l/h in a 10.5°C aquifer. Since bulk electric resistivity variations can bring important information on temperature changes in aquifers (water electric conductivity increases about 2%/°C around 25°C), we monitored the test with surface electric resistivity tomography and demonstrate its ability to monitor spatially temperature variations. Time-lapse electric images clearly show the decrease and then the increase in bulk electric resistivity of the plume of heated water, during respectively the injection and the storage phase. This information enabled to calibrate the conceptual flow and heat model used to simulate the test. Inverted resistivity values are validated with borehole electromagnetic measurements (EM39) and are in agreement with the temperature logs used to calibrate the parameters of the thermo-hydrogeological model for the injection phase. This field work demonstrates that surface electric resistivity tomography can monitor heat and storage experiments in shallow aquifers. These results could potentially lead to a number of practical applications, such as the monitoring or the design of shallow geothermal systems or the use of heated water to replace salt water in tracer tests. [less ▲]

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See detailChange in naming abilities between the ages of 50 and 90: The importance of analyzing naming latency
Verhaegen, Clémence ULg; Poncelet, Martine ULg

in Stem-, spraak- en taalpathologie (2012, September), 17(2), 126-128

This study tests the controversial hypothesis that word naming difficulties may arise in individuals as young as their 50s. Participants of 25-35, 50-59, 60-69 and above 70 years of age were given a ... [more ▼]

This study tests the controversial hypothesis that word naming difficulties may arise in individuals as young as their 50s. Participants of 25-35, 50-59, 60-69 and above 70 years of age were given a picture naming task. To uncover subtle naming difficulties, correct naming latencies were analyzed, in addition to accuracy. Moreover, in order to control whether the expected slower naming latencies could be due to a general slowing affecting all cognitive tasks, participants were also given an odd/even judgment task to assess cognitive processing speed. In participants in their 50s, we found subtle naming difficulties revealed by longer naming latencies, unaccompanied by any decrease in naming accuracy. The age-related naming disadvantage increased with age with the onset of naming errors. Thus, in adults in their 60s and their 70s, the results showed both a decrease in accuracy and an increase in correct naming latencies. Moreover, the increase in naming latencies remained significant even after controlling for odd/even judgment latencies, suggesting a degradation specific to the picture naming task. We assumed that these slower latencies may result from a language-specific impairment. [less ▲]

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See detailGH or IGF-1 : which one is raising blood glucose ? Hints from the LAS (Liege Acromegaly Survey)
PETROSSIANS, Patrick ULg; Zacharieva, S; Chanson, P et al

in Journal of Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3),

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See detailCurrent evidence and indications for Prolotherapy with Platelet Rich Plasma in chronic musculoskeletal conditions
Kaux, Jean-François ULg; Crielaard, Jean-Michel ULg

in Regional Anesthesia & Pain Medicine (2012, September), 37(5 - Suppl 1), 104-106

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See detailPulsational Mode Identification Based on Chromatic Amplitude Behaviour: Recent Results for Rapidly Oscillating Subdwarf B Stars
Randall, Suzanna K; Fontaine, Gilles; Brassard, Pierre et al

in Astronomical Society of the Pacific Conference Series (2012, September), 462(232),

We present recent results from mode identification based on the amplitude-wavelength behaviour for three rapidly pulsating subdwarf B stars: HS 2201+2610, EC 11583-2708 and EC 20338-1925.

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See detailAging of the newly diagnosed acromegalic patients : data frome the LAS (Liege Acromegaly Survey)
PETROSSIANS, Patrick ULg; Zacharieva, S; Chanson, P et al

in Journal für Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3), 33

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See detailBevacizumab for recurrent glioblastoma, early observations on patient outcome in the belgian medical need program
Duerinck, Johnny; Clement, Paul; Bouttens, F et al

in Neuro-Oncology (2012, September), 14(3), 51

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See detailAge, GH and tumor size : the triangular relation in acromegaly. Data from the LAS (Liege Acromegaly Survey)
PETROSSIANS, Patrick ULg; Zacharieva, S; Chanson, P et al

in Journal für Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3), 43

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See detailThe Physics of Pulsating White Dwarf Stars
Fontaine, G.; Brassard, P.; Charpinet, S. et al

in Astronomical Society of the Pacific Conference Series (2012, September), 462

We present a summary of the properties of white dwarf stars, beginning with a brief reminder of their basic characteristics. We continue with a discussion of the spectral types, evolution, and other ... [more ▼]

We present a summary of the properties of white dwarf stars, beginning with a brief reminder of their basic characteristics. We continue with a discussion of the spectral types, evolution, and other properties of cooling white dwarfs, with an emphasis on the internal physics. We then introduce the pulsating white dwarfs and provide an updated view of their status. We finally discuss the impact (real and potential) of asteroseismology on our knowledge of degenerate stars. [less ▲]

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See detailIn vivo antimalarial activity of twigs extracts from Keetia leucantha
Béro, Joanne; Frederich, Michel ULg; Quetin-Leclercq, Joëlle

in Planta Medica (2012, August), 78(11), 1188

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See detailEffect of artificial insemination site on post-mating endometritis in mares
Rodier, Clémentine; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2012, August), 47(suppl 5), 103

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were ... [more ▼]

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were inseminated with a same batch of frozen semen for 3 consecutive cycles. Mares were inseminated with the following procedures in a random order: (1) Deep uterine insemination with 4ml of semen; (2) Horn bifurcation with 4ml of semen; (3) Horn bifurcation with 4ml of semen and 6ml of extender. During each cycle, Cotton (C) and Brush (B) swabs were collected at 4 different moments: mid- dioestrus, mares with a 35mm follicle, 24h and 6 days after AI. Swabs were smeared on slides, fixed and stained (Diff-Quick®) before examination under light microscopy. Proportions of inflammatory and epithelial cells were determined and differences were studied with kruskal-wallis test for non-parametric data. Distensions of uterine lumen due to intraluminal fluid observed during ultrasound exams were measured and recorded. Quality of slides was better (p=0.0006) with B swabs than C swabs with 97% versus 65% of slides readable. B swabs were associated with higher percent of endometrial cells retrieved (p=0.0323), making them a better tool for endometritis diagnosis, which is consistent with our previous reports. Volume of intraluminal fluid and percent of inflammatory cells, both on B and C swabs, were not influenced by AI location regardless the timing of sampling. Small volume deep uterine AI did not significantly affect inflammatory response by the endometrium in our experiment. [less ▲]

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See detailPotential anticancer activity of young Carpinus betulus leaves
Cieckiewicz, Ewa ULg; Angenot, Luc ULg; Gras, T et al

in Planta Medica (2012, August), 78(11), 1178

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See detailEnvironmental enrichment can accentuate condtioned reward induced by representative cocaine doses in mice
Geuzaine, Annabelle ULg; Tirelli, Ezio ULg

in Journal of Psychopharmacology (2012, August), 26(8), 70

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See detailThe effect of homologous prostatic fluid on the parameters of dog semen extended and frozen in Tris-egg yolk and INRA Freeze: preliminary results
Stanescu, Pascal; Van Den Berghe, Femke ULg; Aissi, Adel et al

in Reproduction in Domestic Animals (2012, August), 47(s4), 431

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a ... [more ▼]

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a number of detrimental effects on the frozen-thawed canine semen. The aim of this study was to compare the effect of a LDL commercial extender used for stallion semen – INRA FreezeÒ (IMV Technologies, L¢Aigle, France) with a standard extender for dog semen containing Tris, fructose, glycerol and 20% egg yolk (TFG-EY). Since there are some conflicting reports concerning the effect of prostatic fluid on sperm function, we also investigated if the effect of post-thaw dilution with autologous prostatic fluid on viability, motility and acrosome status of frozen-thawed canine spermatozoa is correlated with the composition of the extender. Semen was collected from six Beagle dogs and each sperm rich fraction was divided in two parts: one was diluted with INRA FreezeÒ and the other with TFG-EY and frozen with the same protocol. The third fraction of each ejaculate was collected separately, centrifuged at 1118 · g for 10 min and the supernatant was frozen at )18°C until use. For each extender, two straws were thawed: one straw was diluted 1:2 with autologous prostatic fluid, while the other was not diluted at all. Motility (CASA, IVOS; Hamilton Thorne, USA), viability and acrosome status (Guava EasyCyteÒ flow cytom- eter; IMV Technologies, L¢Aigle, France), morphology (Diff-Quick stain) were assessed at 5 min, 1 h and 2 h post-thaw (T0, T1, T2). Statistical analyses were performed with IBM SPSS software (ver. 19 for Windows; IBM, New York, NY, USA). The results are presented as mean values and a p value <0.05 was considered statistically significant. Viability, total and progressive motility and the percentage Ó 2012 Blackwell Verlag GmbH 432 Abstracts of intact acrosomes were higher (p < 0.05) for the TFG-EY group with or without the addition of the prostatic fluid. The results for motility in the INRA Freeze group were dramatically low compared to the TFG-EY group. There were no significant differences regarding the morphology of fresh and frozen semen for any of the four groups. Addition of prostatic fluid for the TFG-EY group significantly reduced the total and progressive motility at T0, T1 and T2 (p < 0.05), but showed no influence for the INRA Freeze group. This data show that the effect of prostatic fluid regarding motility differs between the two extenders. This may illustrate either an inhibitory effect of some components of the INRA FreezeÒ on the noxious effects of the prostatic fluid or conversely an enhancing effect of the TFG-EY on those. Alternatively, it could be that low values obtained with INRA FreezeÒ did not permit highlighting the effects of prostatic fluid. [less ▲]

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See detailMeasurement of human levels of Dechlorane 602 by CZC-NCI-HRTOFMS
Brasseur, Catherine ULg; PIRARD, Catherine ULg; L'Homme, Benjamin ULg et al

in Organohalogen Compounds (2012, August), 74

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See detailMyeloperoxidase as an indicator of endometritis in the mare: preliminary results
Parrilla Hernandez, Sonia ULg; Franck, Thierry ULg; Ponthier, Jérôme ULg et al

in Reproduction in Domestic Animals (2012, August), 47(suppl 5), 65

Diagnosis of endometritis in the mare is routinely based on the presence of polymorphonuclear cells (PMNs) on endometrial smears. Studies show a relation between PMNs and myeloperoxidase (MPO), an enzyme ... [more ▼]

Diagnosis of endometritis in the mare is routinely based on the presence of polymorphonuclear cells (PMNs) on endometrial smears. Studies show a relation between PMNs and myeloperoxidase (MPO), an enzyme released by PMNs during degranulation or after cell lysis, in many fluids and tissues. The aims of this study were to assess the presence and concentration of MPO in the mare’s uterus, and to investigate its relation with PMNs. Thirty-six cycles from 28 mares (ages ranging from 6 to 22 years) were used. Endometrial cytological samples were obtained with a small volume uterine flush and either a uterine cotton swab or a cytobrush, when a follicle >35 mm was observed by ultrasound. The smears were stained with Diff-QuickÒ and one or more PMNs per field (400·) was diagnosed as endometritis. The supernatant of the flushes was used to measure MPO concentration with a specific equine MPO ELISA assay. Our results showed the presence of MPO in the equine uterus during oestrus (mean = 2839 + 2785). MPO concentrations were signifi- cantly (p < 0.05) higher in samples with positive cytological results. Occasionally, some samples with negative cytological results showed high MPO concentration, but the opposite was never observed. Clinical signs of endometritis are not always present, or they may be delayed. An early diagnostic improves the success of treatment. Our results show that high quantities of MPO in endometrial samples indicate the presence of PMNs. Further studies are needed to determine if MPO concentration could be routinely used as a tool of early detection of endometritis. [less ▲]

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See detailA flow cytometric study on the effect of myeloperoxidase on stallion spermatozoal motility and structure
Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg; Van Den Berghe, Femke ULg et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 509

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1 ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1]. The aim of the study was to determine effects of experimental addition of active MPO on motility, mitochondrial potential, apoptosis induction, membrane and acrosome integrity in equine semen. Three stallions were used and semen was collected four times. Extended (INRA96TM) semen was processed for density gradient centrifugation (Equipure Bottom Layer®) [2]. Purified pellet was re-extended to 100x106spermatozoa/ml in INRA96TM and divided in 3 samples. One sample was used for control and active human MPO (Calbiochem, Merck) was added in the other two samples to final concentration of 5 or 50 ng/ml. After incubation (2 hours, 20°C), motility was analysed with Computer Assisted Semen Analysis (IVOS, Hamilton-Throne, Beverly, MA, USA) and cytometric analyzes were perfomed with EasyCyte (IMV). Mitochondrial potential and apoptosis were assayed using Guava Mitopotential JC-1 and 7-AAD kit (Millipore). Membrane and acrosome integrity were respectively assayed with PI (Propidium Iodide) (Invitrogen) and PNA (Peanut Agglutinin-Fluorescein Iso Thio Cyanate) (Sigma-Aldrich). Statistical differences (p<0.05) were determined using Kruskall-Wallis test. No effect of the stallions was observed on parameters assayed in this study. Unlike total motility, progressive motility was decreased in both MPO concentrations (p<0.001). MPO addition had no effect on membrane and acrosome integrity. No differences were detected for the percentages of spermatozoa having polarised or depolarised mitochondria. Apoptosis, assayed by 7-AAD fluorescence, was not increased by the treatments. Our results agree with previously published effects of in vitro ROS production systems with xanthine oxidase [3], showing an effect on motility but no influence on mitochondria and membrane or acrosome integrity. However, membrane lipoperoxidation was increased by ROS in this study [3], and it could be linked to the impaired motility also observed in our protocol. Further studies with increasing concentrations of added MPO should be conducted to correlate motility with lipoperoxidation. References [1] Ponthier J, Desvals M, Franck T, de la Rebiere de Pouyade G, Spalart M, Palmer E, Serteyn D, Deleuze S. Myeloperoxidase in equine semen: Concentration and Localization during freezing processing. Journal of Equine Veterinary Science 2012;32: 32-37. [2] Edmond AJ, Teague SR, Brinsko SP, Comerford KL, Waite JA, Mancill SS, Love CC, Varner DD. Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa. Animal Reproduction Science 2008;107: 318-318. [3] Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MC. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J Androl 2000;21: 895-902. [less ▲]

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See detailAnalytical criteria for use of MS/MS for determination of dioxins and dioxin-like PCBs in feed and food
Kotz, A; Malisch, R; Focant, Jean-François ULg et al

in Organohalogen Compounds (2012, August), 74

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See detailRaw semen concentration directly influences CASA velocity pathways
Parrilla Hernandez, Sonia ULg; Deleuze, Stefan ULg; Van Den Berghe, Femke ULg et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 505

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data ... [more ▼]

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data. That stallion effect may have interfered with the associations we observed as stallions seem to have specific concentration and motility pathways. More studies, with more replicates, will allow comparing results from a same stallion and further establish the correlations we report here. [less ▲]

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See detailSquamous metaplasia of the prostate and diffuse alopecia in a 13-year-old castrated dog due to chronic ingestion of exogenous estradiol
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2012, August), 47(S4), 561

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to ... [more ▼]

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to exclude or confirm Cushing disease. The dog had previously been treated with trilostane for 4 months without having any positive effects. Earlier blood analysis showed no significantly abnormalities and ACTH stimulation test was negative. Having bilateral cryptorchidism, the dog was castrated at young age. Clinical findings and treatment. Abdominal ultrasound revealed a slightly enlarged right adrenal gland (diameter of 1 cm at the cranial part). The prostate was enlarged and heterogeneous with multiple anechogenic cavities, indicating hormonal stimulation. Craniad the prostate and ventrally of the bladder, a hypoechogenic, oval structure (3 cm by 1.7 cm) could be seen, and was suspected to be a remaining cryptorchide testicle. Fine needle aspiration however showed that the structure contained a thick purulent liquid, with large amounts of polymorhonuclear white blood cells. Blood analysis revealed a leukocytosis (29370/mm3) with neutrophilia (23202/mm3). Low-dose dexamethasone test definitely excluded Cushing disease (basal cortisol levels 2,17 µg/dl; cortisol at 4 and 8 hours < 1 µg/dl). A prostatic wash was performed and revealed a severe prostatitis and presence of keratinized prostatic cells, indicating a squamous metaplasia of the prostate. Measurement of seric LH levels (LH Witness®, Synbiotics) showed an LH concentration lower than 1 ng/ml, indicating a hormonal negative feedback on the hypothalamo-hypophyso-gonadal axis. After a more thorough anamnesis, it became clear that the dog was licking and ingesting a transdermal estradiol containing cream (Estrogel®) from his owner, since two years, causing his symptoms. The dog was put on antibiotic treatment for the prostatitis (enrofloxacine 10 mg/kg SID) and underwent surgery to excise the abscess cranial of the prostate. The two ductuli deferentes were attached to this structure. Histopathological analysis revealed this tissue to be embryological remanents or a morphological anomaly with a urogenital origin. Control after 4 weeks showed that the prostate slightly decreased in volume, however, anechogenic cavities where still present of which one increased in volume. The alopecia was still present as well, both probably due to a prolonged action of the estrogens. Conclusion. Even though injectable estrogen preparations are not longer available in veterinary medicine in Europe, a thorough anamnesis towards other exogenous estrogen sources is still necessary and can reduce the number of supplementary exams performed. [less ▲]

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See detailBenveniste serait-il aujourd’hui un linguiste de l’énonciation ?
[Lttr 13]; Badir, Sémir ULg; Polis, Stéphane ULg et al

in Arts et savoirs (2012, July 24), 2

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See detailMALDI MSI for ovarian cancer biomarkers research: latest developments of the technology for screening and tracking.
Longuespée, Rémi ULg

in Histochemistry & Cell Biology (2012, July), 138(1), 141-54

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See detailEffect of the milk recording time on the genetic parameters of milk production and mid-infrared milk components in Luxembourg dairy cattle
Arnould, Valérie ULg; Gengler, Nicolas ULg; Soyeurt, Hélène ULg

in Journal of Dairy Science (2012, July), 95

A particularity of the Luxembourg milk recording is the use of different schemes. Two principal schemes are applied: the scheme “S” applied on 69.1% of the total herds (n=712) and consisting in one ... [more ▼]

A particularity of the Luxembourg milk recording is the use of different schemes. Two principal schemes are applied: the scheme “S” applied on 69.1% of the total herds (n=712) and consisting in one proportionate sample of all daily milkings, and the scheme “T” (21.6% of the total herds) which consists in one sample of only one milking (morning or evening milking) (and alternating milking time from month to month). The problematic is that application of different schemes could influence the milk components (protein and fat yield) and the milk fat components (saturated and unsaturated groups of fatty acids) genetic parameters estimation and to prevent all comparisons between dairy population under different milk recording schemes. A total of 47,613 and 44,833 test-day records were obtained, respectively for schemes “S” and “T” from Holstein cows in first lactation in Luxembourg dairy herds. The used model included as fixed effects: herd x date of test, class of age, and month x year. Random effects were permanent environmental, additive genetics, and residual effects. The main objective of this work is to study the effect the choice of milk recording schemes (“S” or “T” schemes) on milk yield and milk components genetic parameters. A solution could be to add a fixed effect taking in account the milking time. The second objective is to study the effect of milking time (morning or evening) on genetic parameters estimated in the case of scheme “T”. According to the results, genetic parameters were statistically different between the schemes “S” and “T” for milk yield (P value < 0.0001). Further, the classifications of bulls according to their breeding values were very different when values were estimated on basis of scheme “S” or “T” (Spearman correlation value of 0.51 for milk yield for example). In conclusion, using several milk recording schemes do not allow any comparison of genetic parameters between dairy cattle’s. [less ▲]

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See detailMALDI MSI for ovarian cancer biomarkers research: latest developments of the technology for screening and tracking.
Longuespée, Rémi ULg

in Histochemistry & Cell Biology (2012, July), 138(1), 141-54

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See detailInvestigation on the High Efficiency Volume Bragg gratings performances for spectrometry in space environment: Preliminary results
Loicq, Jerôme ULg; Georges, Marc ULg; Gaspar Venancio, Luis Miguel

in Telescope and systems (2012, July)

The special properties of Volume Bragg Gratings (VBGs) make them good candidates for spectrometry applications where high spectral resolution, low level of straylight and low polarisation sensitivity are ... [more ▼]

The special properties of Volume Bragg Gratings (VBGs) make them good candidates for spectrometry applications where high spectral resolution, low level of straylight and low polarisation sensitivity are required. Therefore it is of interest to assess the maturity and suitability of VBGs as enabling technology for future ESA missions with demanding requirements for spectrometry. The VBGs suitability for space application is being investigated in the frame of a project led by CSL and funded by the European Space Agency. The goal of this work is twofold: first the theoretical advantages and drawbacks of VBGs with respect to other technologies with identical functionalities are assessed, and second the performances of VBG samples in a representative space environment are experimentally evaluated. The performances of samples of two VBGs technologies, the Photo-Thermo-Refractive (PTR) glass and the DiChromated Gelatine (DCG), are assessed and compared in the H, O2-B and NIR bands. The tests are performed under vacuum condition combined with temperature cycling in the range of 200 K to 300K. A dedicated test bench experiment is designed to evaluate the impact of temperature on the spectral efficiency and to determine the optical wavefront error of the diffracted beam. Furthermore the diffraction efficiency degradation under gamma irradiation is assessed. Finally the straylight, the diffraction efficiency under conical incidence and the polarisation sensitivity is evaluated. [less ▲]

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See detailA bone remodelling model for long term orthodontic tooth movement.
Mengoni, Marlène ULg; D'Otreppe, Vinciane ULg; Ponthot, Jean-Philippe ULg

in Journal of Biomechanics (2012, July), 45(Supplement 1), 180

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See detailMALDI MSI for ovarian cancer biomarkers research: latest developments of the technology for screening and tracking.
Longuespée, Rémi ULg

in Histochemistry & Cell Biology (2012, July), 138(1), 141-54

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See detailThe situation of gerontechnology in Belgium
Petermans, Jean ULg; LEJEUNE, CHRISTOPHE; GILLAIN, Daniel ULg

in GERONTECHNOLOGY (2012, June 26), 11(2), 269-270

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See detailSeverity of incident vertebral fracture and future fracture risk: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 716

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See detailPrise en charge des brûlés en phase aigue : enquête européenne.
ROUSSEAU, Anne-Françoise ULg; LEDOUX, Didier ULg; MASSION, Paul ULg et al

in Brûlures. Revue Française de Brûlologie (2012, June), XIII(2), 60

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See detailGranulomatous meningo-encephalitis caused by Toxoplasma gondii in three bulls, a possible explanation for unexplained sporadic bovine meningo-encephalitis
Theron, Léonard ULg; Tabaran, F; Cassart, Dominique ULg et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailSevere prevalent vertebral fractures predict subsequent vertebral and nonvertebral fractures: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 588

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See detailChildren of lesbian parents: Research on family triadic interactions
Miscioscia, Marina ULg; Scarciotta, Lidia; D'Amore, Salvatore ULg et al

in Infant Mental Health Journal (2012, June), 33(3 Abstract Supplement), 26

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See detailRadiological and clinical profil of osteoarthritic patients undergoing of total joint replacement
Neuprez, Audrey ULg; François, Garance ULg; Bruyère, Olivier ULg et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 693

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See detailInvestigation of body condition score and insulin sensitivity (RQUICKY) in downer cow syndrome
Bonvoisin, Marie ULg; Rollin, Frédéric ULg; Lebreton, Pascal et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailAssessment of quality of life in patients undergoing total joint replacement for OA of the lower limb
Neuprez, Audrey ULg; François, Garance ULg; Bruyère, Olivier ULg et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 693

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See detailInvestigation of sudden death of calves after epileptic seizures due to hypomagnesaemia and hyperparathyroidism
Chevalier, Marine ULg; Lebreton, Pascal; Garnier, Catherine et al

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

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See detailPerception, knowledge and use by general practitioners of Belgium of the FRAX tool
Bruyère, Olivier ULg; Nicolet, Delphine ULg; Compère, Stéphanie et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 716

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See detailEffect of mechanical weeding on wild Chamomile populations in winter wheat crop
Jaunard, Delphine ULg; Monty, Arnaud ULg; Henriet, François et al

in Communications in Agricultural and Applied Biological Sciences (2012, May 22)

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See detailEfficacy of a strontium ranelate 2 G/vitamin D3 1000 UI combination on the correction of vitamin D insufficiency
Rizzoli, R; Dawson-Hughes, B; Kaufman, JM et al

in Osteoporosis International (2012, May), 23(S2), 225

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See detailLiège experience in donation after cardiac death liver transplantation: 2003-2011
Le Dinh, Hieu ULg; DELWAIDE, Jean ULg; MONARD, Josée ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 6811

Objectives: Results of DCD-LT at the University Hospital of Liège were evaluated from 2003 to 2011. Methods: Medical records of 56 DCD liver recipients were retrospectively reviewed with regard to patient ... [more ▼]

Objectives: Results of DCD-LT at the University Hospital of Liège were evaluated from 2003 to 2011. Methods: Medical records of 56 DCD liver recipients were retrospectively reviewed with regard to patient and graft survivals and biliary complications. Mean follow-up was 26.4 months. Mean donor age was 56.3±14.5 years (25 - 83). Donor causes of death were due to anoxia (51.8%), stroke (32.1%) and head trauma (14.3%). Mean WIT, CIT and suture time were 20.5±7.1min (10 – 39), 265.6±85.1min (105 – 576), and 40.8±7.8 min (25 – 61), respectively. 95% of liver grafts were locally shared. HTK was the most commonly used perfusion solution (86%). Mean recipient age was 56.6±10.5 years (29 – 73). Indications for LT included ESLD (53.6%) and HCC (46.6%). Mean MELD score at transplant was 15.6±6.1points (6 – 40). Results: No primary non-function grafts. Mean peak serum AST and bilirubin levels were 2520±3621UI/L and 50.2±49.2mg/L, respectively. Eight patients (14.3%) developed biliary complications. No intra-hepatic bile duct strictures or re-transplantation. Global patient and graft survival was 92.6% at 3 months, 92.6% at 1 year, 73.8% at 3 years and 60% at 5 years. Death-censored patient and graft survival at the corresponding time points was 92.6%, 92.6%, 87.7% and 87.7%. Thirteen liver grafts were lost during follow-up exclusively due to recipient deaths. The rate of HCC recurrence was 33.3%. Conclusions: Controlled DCD donors are a valuable source of transplantable liver grafts. Primary results are encouraging and apparently as good as those from brain-dead donation LT essentially due to short WIT and CIT. [less ▲]

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See detailAutomatic real-time collection of RCS of airplanes in a real bistatic low-frequency configuration using a software defined passive radar based on illuminators of opportunity
Pisane, Jonathan ULg; Azarian, Sylvain; Lesturgie, Marc et al

in Proceedings of the 2012 IEEE Radar Conference (2012, May)

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See detailResults of kidney transplantation from controlled donors after cardio-circulatory death: a single center experience
Le Dinh, Hieu ULg; WEEKERS, Laurent ULg; BONVOISIN, Catherine ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 667

Objectives: The aim of this study was to determine results of kidney transplantation (KT) from controlled donation after cardio-circulatory death (DCD). Primary end-points were graft and patient survival ... [more ▼]

Objectives: The aim of this study was to determine results of kidney transplantation (KT) from controlled donation after cardio-circulatory death (DCD). Primary end-points were graft and patient survival, and post-transplant complications. The influence of delayed graft function (DGF) on graft survival and DGF risk factors were analyzed as secondary end-points. Methods: This is a retrospective mono-center review of a consecutive series of 80 DCD-KT performed at the University Hospital of Sart Tilman, University of Liège, between Jan 2005 and Dec 2011. Mean patient follow-up was 28.5 months. Results: Overall graft survival was 93.7%, 89.5%, 85% and 81.3% at 3 months, 1 year, 3 and 5 years, respectively. Death-censored graft survival at the corresponding time points was 93.7%, 93.7%, 90.8% and 90.8%. Main cause of graft loss was patient’s death with a functioning graft. No primary non-function grafts were encountered. Renal graft function was suboptimal at hospital discharge, but nearly normalized at 3 months. DGF was observed in 36% of all DCD-KT. DGF significantly increased post-operative length of hospitalization, but had no deleterious impact on graft function or survival. Donor body mass index (BMI) ≥30 kg/m2, recipient BMI ≥30 kg/m2 and pre-transplant dialysis duration significantly increased the risk of DGF in a multivariate logistic regression analysis (p < 0.05). Conclusions: Despite a higher rate of DGF, controlled DCD-KT offers a valuable contribution to the pool of deceased donor kidney grafts, with comparable mid-term results to those procured after brain death. [less ▲]

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See detailDREAM 2012: DEVELOPMENT OF LAPAROSCOPIC SURGERY AT THE UNIVERSITY HOSPITAL OF KINSHASA, DRC
Nsadi Fwene, Berthier ULg; Veyi, D; Kazadi, J et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8240

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that ... [more ▼]

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that laparoscopy may be developed in the Cliniques Universitaires de Kinshasa (CUK), and may be cost-effective. The final aim of this program is to bring the benefits of laparoscopy to the DRC population, by allowance of adequate training on the UNIKIN personnel, including anaesthetists, surgeons and nurses, who in the future will have to locally form the DRC medical and nursery students. Methods: With the financial support from Wallonie-Bruxelles International (WBI), a complete CUK team, including a surgeon (2 years training in Belgium), an anaesthetist and nurses, were trained in Belgium and then afterwards in DRC. The laparoscopic equipment was sent to Kinshasa, and three theoretical and practical missions of Belgian teams were organised. Results: Over a 2 year period, 116 laparoscopic procedure were performed, including 32 appendectomies, 41 cholecystectomies, 11 hernia repairs, 9 laparoscopy explorations for peritoneal carcinoma assessment and biopsy, 8 procedures for catheter of dialysis peritoneal, 5 gynecologics procedures, and 10 other miscellaneous procedures. Conclusions: A joined approach, taking into account on one hand the training of the skills locally trained to adapt itself to some difficulties, on the other hand institutions of scientific support and a real program and local will of development of this new procedure are the wages of development, accessibility and durability of such news approach in developing countries. All University and non-University team willing to join such a project are welcome. [less ▲]

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See detailINTRAPERITONEAL ADHESIONS AFTER OPEN OR LAPAROSCOPIC ABDOMINAL PROCEDURE: AN EXPERIMENTAL STUDY IN THE RAT
Arung, Willy; Drion, Pierre ULg; CHERAMY-BIEN, Jean-Paul ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8546

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 ... [more ▼]

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 pneumoperitoneum is also largely discussed. This study aimed to compare adhesion formation following peritoneal injury by electrocoagulation performed through open or laparoscopic procedures in a rat model. Methods: Sixty male rats were randomized to undergo a 1.5cm peritoneal injury with unipolar cautery under general anesthesia: open surgery (group A, n=20), laparoscopic surgery with CO2 pneumoperitoneum (group B, n=20) and laparoscopic surgery with air pneumoperitoneum (group C, n=20). Duration of the procedures was fixed at 90 minutes in all groups, and pneumoperitoneum pressure at 10mmHg. Ten days later, the animals underwent a secondary laparotomy to score peritoneal adhesions using qualitative and quantitative parameters. Results: Forty-five rats developed at least one adhesion, respectively 95% in group A, 83% in group B and 55% in group C (P<0.01; Group C vs Group A, P<0.01). According to number, thickness, tenacity, vascularization, extent, type, and grading according to Zühkle classification, no significant difference was observed between groups A and B. The distribution of adhesions after open surgery was significantly different than after laparoscopic surgery (P<0.001). Interestingly, group C rats developed significantly less adhesions at the traumatized site, and their adhesions had less severe qualitative scores compared to open surgery (P<0.01). Conclusions: In this animal model, CO2 laparoscopic surgery did not decrease the formation of postoperative adhesion, compared to open surgery. The difference with the animals operated with air pneumoperitoneum emphasizes the role of CO2 in peritoneal injury leading to adhesion formation. [less ▲]

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See detailWhat is the potential increase of the heart graft pool by cardiac donation after circulatory death?
NOTERDAEME, Timothée; HANS, Marie-France ULg; NELLESSEN, Eric ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 668

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See detailINTRA-TUMORAL HETEROGENEITY AND RATIONAL SELECTION OF ANTIGENS FOR TARGETED THERAPY OF LIVER METASTASES
Turtoi, Andrei ULg; Blomme, Arnaud ULg; Delvaux, David ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8953

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer ... [more ▼]

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer cells causing treatment resistance and relapse. Therefore, a rational strategy is needed to select targetable antigens that would overcome this intra-tumoral heterogeneity. Methods: After ethical committee approval, 48 fresh liver metastases of colorectal origin were prospectively collected from patients undergoing liver resection. Here we macroscopically divided the lesion in different zones and generated a unique quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. Particular focus was laid on accessible proteins, a protein subclass comprising cell membrane associated and extracellular proteins. Accordingly, the tissues were ex-vivo biotinylated, affinity purified and analyzed for each zone separately using nano-UPLC-MSe proteomics technique. In total over 1500 unique proteins were statistically divided into different patterns of expression. Results: We have generated a quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. The study offers insight into novel targets but also antigens against which the antibodies are already involved in clinical trials or treatment of liver metastases. Extensive clustering and validation experiments highlight novel markers that offer the potential to homogeneously cover the metastatic lesion and become better targets. Conclusions: Two such antigens, LTBP2 and TGFBI were selected for functional analysis in colorectal carcinoma cells. In vitro and in vivo experiments showed that in particular TGFBI is relevant for migration and proliferation capacity of colorectal cancer cells. The suppression of this protein led to significant inhibition of tumor growth, crystalizing it as bona fide target for the development of anti-metastases therapies. [less ▲]

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See detailEFFECT OF PARECOXIB, A SELECTIVE COX-2 INHIBITOR, IN THE PREVENTION OF POSTOPERATIVE ADHESIONS IN A RAT MODEL
Arung Kalau, Willy ULg; Drion, Pierre ULg; CHERAMY-BIEN, Jean-Paul ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8444

Objectives: Postoperative peritoneal adhesions are frequent after abdominal surgery. Many preventive agents have been tried in animal models and in clinical trials, but up to now, there has been no ... [more ▼]

Objectives: Postoperative peritoneal adhesions are frequent after abdominal surgery. Many preventive agents have been tried in animal models and in clinical trials, but up to now, there has been no definitive strategy to prevent their formation. In this study, the effectiveness of parecoxib (Dynastat®), a selective cyclooxygenase-2 inhibitor, in preventing experimental intra-abdominal adhesions in rats was studied. Methods: Thirty male rats who underwent a primary surgical procedure aiming at inducing peritoneal injury to produce intraabdominal adhesion, were randomized in three groups: (A) control group, no therapy; (B) intraperitoneal (IP) parecoxib group; (C) intramuscular (IM) parecoxib group. Ten days later, a xyphopubic midline incision was performed and the whole abdominal cavity was explored to score the peritoneal adhesions. Results: Twenty-three rats developed adhesions, 9 (100%) in group A, 7 (70%) in group B and 4 (40%) in group C (P=0.01). The extent and severity scores of adhesion were significantly lower in groups B and C than those in control group (p<0.001). Type of adhesions was measured at 2.25 ± 0.67 in group A, 1.20 ± 0.86 in group B and 0.82 ± 0.80 in group C. This was significantly different between group A and B (p<0.001), between A and C (p<0.001). But no significant difference was found between group B and C (p=0.17). Conclusions: In this study, we found a significant effect on parecoxib in the prevention of postoperative adhesions. But, without avoiding completely the formation of adhesion, parecoxib reduces significantly extent and severity of postoperative adhesions in rats treated with IP or IM parecoxib administration. [less ▲]

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See detailLaparoscopic liver resection: a single center experience
SZECEL, Delphine ULg; DE ROOVER, Arnaud ULg; DELWAIDE, Jean ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 631

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See detailRisk factor of fetal chromosomal aberrations in the south of Vietnam
To, Hong ULg

in European Federation of Societies for Ultrasound in Medicine and Biology (2012, April 22)

Objectives: The aim of this study is to find the risk factors of fetal chromosomal aberrations in order to classify a high risk pregnancy in our prenatal care unit. Material and methods: A prospective ... [more ▼]

Objectives: The aim of this study is to find the risk factors of fetal chromosomal aberrations in order to classify a high risk pregnancy in our prenatal care unit. Material and methods: A prospective study was carried out during one year in our clinic. 523 cases of amniocentesis was followed up and evaluated the relation with abnormal result (fetal aneuploidies) and the risk factors such as advanced maternal age (≥ 35 years old), parity, habitation, positive triple test (≥ 1/250), fetal malformation history and abnormal signs in the first and second trimester scanning. Results: The incidence of fetal aneuploidy was 3.4% (18/523 [95%CI: 2.1-5.4%]) and the trisomy 21 showed the most common in these chromosomal aberrations (67%). The younger women (< 35 years old), nulliparous, positive triple test and abnormal ultrasonographic findings (included increased nuchal translucency [≥ 2.4mm], absent nasal bone in the first trimester scanning and/or echogenic nodule in ventricular) was identified as the risk factors of fetal aneuploidies (OR was 2.3 [95%CI: 0.8-6.7], 4.0 [95%CI: 1.4-13.2], 3.3 [95%CI: 0.3-33.5] and 9.4 [95%CI: 1.1-79.2], respectively). The abnormal ultrasonographic findings found a relation significantly with abnormal chromosomes (p=0.04). Conclusion: Abnormal ultrasonographic findings in the first and second trimester were the important markers for predicting fetal aneuploidy, especially trisomy 21. [less ▲]

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See detailFeasibility of scanning fetal anatomy in the first trimester of gestation
To, Hong ULg

in European Federation of Societies for Ultrasound in Medicine and Biology (2012, April 22)

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and ... [more ▼]

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and methods: In a prospective study, 2500 singleton pregnancies measured fetal NT and scanned structural anatomy in the first trimester; then checked fetal morphology systematically at 18-24 weeks and followed up to their delivery. According to ultrasonographic abnormalities and amniocentesis, we evaluated the detection rate of ultrasound for aneuploidy in the first and second trimester of gestation. Results: The sensitivity and specificity of ultrasound for aneuploidy was 17.2% and 99.7%, respectively. All 13 cases of fetal ultrasonographic anomalies related significantly with an increased NT (≥ 2.4mm) (p < 0.001). 30.8% of these abnormalities that included cystic hygroma, omphalocele and holoprosencephaly were detected at 11-13.6 weeks’ gestation. In the second trimester scanning, 69.2% of major structural anomalies found and the detection rate of central nervous system malformation, abnormal heart, abdominal wall defect, face anomaly and skeletal deformities were 85.7%, 100%, 66.7%, 50% and 75%, alternatively. Conclusion: Scanning of fetal anatomy at the time of NT measuring is useful to detect fetal abnormalities, especially aneuploidy in Vietnamese pregnancies. [less ▲]

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See detailLa bande dessinée belge avant Hergé. Quelle histoire ?
Paques, Frédéric ULg

in Comicalités : Études de Culture Graphique (2012, April 11)

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See detailExpression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates
Lambert, Cécile ULg; Dubuc, Jean-Emile; Hennuy, Benoît ULg et al

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. <br />Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. <br />Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. <br />Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

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See detailA prospective study of cardiac valvular status in patients treated with cabergoline for Endocrine Disease
VROONEN, Laurent ULg; Lancellotti, Patrizio ULg; Tome Garcia, M et al

in Annales d'Endocrinologie (2012, April), 73(2), 148

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See detailHumidity 3D field comparisons between GNSS tomography, IASI satellite observations and ALARO model
Brenot, Hugues; Champollion, Cedric; Deckmyn, Alex et al

in Geophysical Research Abstracts (2012, April), 14

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See detailClinical characterization of cabergoline resistant prolactinomas : a multicenter experience on 92 patients
VROONEN, Laurent ULg; Jaffrain Rea, ML; PETROSSIANS, Patrick ULg et al

in Annales d'Endocrinologie (2012, April), 73(2), 153

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See detailEvaluation of three immunoassays for serodiagnosis of human Mycoplasma pneumoniae infection
HUYNEN, Pascale ULg; TOUSSAINT, Françoise ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2012, April), 18(S3), 231

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See detailTHE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION
Shafiei, Rasoul ULg; Thonart, Philippe ULg

in Acetic Acid Bacteria (2012, April)

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular ... [more ▼]

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular functions of acetic acid bacteria. In previous studies in CWBI, Acetobacter senegalensis was used for production of dried vinegar starters; however the impact of stressors (ethanol and acetic acid) on A. senegalensis remained unclear. In this study, different techniques such as flow cytometry, culturability on solid medium and 2-DiGE were used comparatively to investigate the effect of carbon sources of inoculum media on the tolerance of A. senegalensis to stressors. Analysis of respiration system by flow cytometric methods showed that the presence of 2% (v/v) acetic acid in inoculum medium, in one hand, causes 80% of cells to continue to do respiration after a sudden exposure to 1- 3% (v/v) acetic acid in stress media while 89.7% of cells grown in glucose appeared as dead cells after an abrupt exposure to 3%(v/v) of acetic acid. On the other hand, 59.2% and 49.33% of cells grown in the presence of 2% (v/v) of acetic acid could maintain their entire membrane integrity after exposure to 1% and 3% (v/v) of acetic acid, respectively. Inoculum medium contained 5% (v/v) of ethanol as a carbon source enabled about 90% of cells to keep their growing capacities after a sudden exposure to 3% acetic acid. In contrast, just 40% of cells grown in glucose as a carbon source maintained their culturability on solid medium after exposure to 1% acetic acid. A similar profile of culturability was observed for the cells grown in 5% (v/v) ethanol or 2% (v/v) of acetic acid. A proteomic approach (2-DiGE) was used to analyze proteins expressed in the presence of different carbon sources. Differentially expressed proteins were mainly associated with energy metabolism, carbohydrate metabolisms, folding, sorting and degradation processes. The relative abundance of proteins was extensively different for cell grown in glucose compared with protein contents of cells grown in ethanol or acetic acid. In conclusion, production of a cost effective vinegar starter needs a qualified biomass which tolerates ethanol and acetic acid. Tolerance of A. senegalensis to acetic acid depends to a great extent on the composition of the medium which cells grow in. In spite of low adaption to acetic acid for cell grown in glucose, using ethanol or acetic acid in inoculum media renders a physiological state in A. senegelensis which enables it to cope with higher concentration of acetic acid readily, this biomass has a potential to be used as a starter [less ▲]

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See detailAMICE: erste internationale Modellrechnung der Maas
Becker, Bernhard; Patzke, Simone; Detrembleur, Sylvain ULg et al

in Forum für Hydrologie und Wasserbewirtschaftung (2012, March 22), 12(31), 165-170

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den ... [more ▼]

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den Anrainerstaaten Frankreich, Belgien, Deutschland und der Niederlande vorgehaltenen numerischen Modelle verwendet. Die Randbedingungen dieser Modelle wurden in einem iterativen Prozess abgeglichen. Die Ergebnisse der zusammenhängenden Simulation tragen zunächst zu einem besseren Systemverständnis bei. Im weiteren Verlauf des Projektes fließen sie in eine Risikobetrachtung ein und werden zur Bewertung von Maßnahmen zur Reduzierung des Hochwasserrisikos vor dem Hintergrund zu erwartender Änderung der Bemessungsgrößen infolge einer erwarteten Klimaänderung herangezogen. [less ▲]

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See detailLA FALSIFICATION DES MEDICAMENTS
Marini Djang'Eing'A, Roland ULg

in Forum Pharmaceutique (2012, March 16), -(-), 16

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See detailHigh-resolution spectropolarimetric observations of hot subdwarfs
Petit, P.; Van Grootel, Valérie ULg; Bagnulo, S. et al

in Leaflet - Astronomical Society of the Pacific (2012, March 01), 452

We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I ... [more ▼]

We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I and V line profiles to enhance the signal-to-noise ratio. We then average all available cross-correlation profiles of each star to further decrease the noise level. Although both targets were previously reported to host kilo-gauss magnetic fields, we do not derive any evidence of large-scale photospheric fields from our sets of observations, in spite of tight error bars on the longitudinal field of the order of 60 gauss for HD 76431 and 200 gauss for Feige 66. A new analysis of FORS1 observations of HD 76431, which provided the basis for the original claim of field detection, confirms the absence of any detectable Zeeman signature, with an error bar of about 100 gauss on the longitudinal magnetic field. [less ▲]

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See detailAutomata-Based Symbolic Representations of Polyhedra
Boigelot, Bernard ULg; Brusten, Julien ULg; Degbomont, Jean-François ULg

in Lecture Notes in Computer Science (2012, March), 7183

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See detailStrontium ranelate reduces the number of radiological or radioclinical progressors in patients with primary knee osteoarthritis
Reginster, Jean-Yves ULg; Chapurlat, R; Christiansen, C et al

in Osteoporosis International (2012, March), 23(S2), 366-367

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See detailWhat are the most important medication attributes for patients with osteoporosis ? Results from a qualitative study
Hiligsmann, Mickaël ULg; Van Durme, C; Geusens, P et al

in Osteoporosis International (2012, March), 23(S2), 81-82

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See detailEfficacy of once-yearly zoledronic acid 5 mg in men with osteoporosis with different levels of serum total testosterone
Boonen, S; Reginster, Jean-Yves ULg; Kaufman, JM et al

in Osteoporosis International (2012, March), 23(S2), 79-80

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See detailDenosumab treatment of postmenopausal women with osteoporosis for 6 years : results from the first 3 years of the freedom extension
Papapoulos, S; Brown, JP; Chapurlat, R et al

in Osteoporosis International (2012, March), 23(S2), 76

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See detailAMP-activated protein kinase controls liposaccharide-induced hyperpermeability
Castanares-Zapatero, Diego; Overtus, M; Communi, Didier et al

in Critical Care (2012, March), 16(suppl 1), 17

Organ dysfunction determines the severity of sepsis and is correlated to mortality. Endothelial increased permeability contributes to the development of organ failure. AMP-activated protein kinase (AMPK ... [more ▼]

Organ dysfunction determines the severity of sepsis and is correlated to mortality. Endothelial increased permeability contributes to the development of organ failure. AMP-activated protein kinase (AMPK) has been shown to modulate cytoskeleton and could mediate endothelial permeability. Our hypothesis is that AMPK controls sepsis-induced hyperpermeability in the heart and is involved in septic cardiomyopathy. Sepsis was induced by intraperitoneal injection of liposaccharide, 10 mg/kg (LPS). Alpha-1 AMPK knockout mice (α1KO) were compared with wild-type. Vascular permeability was characterized by Evans blue extravasation. Inflammatory cytokine mRNA expression was determined by qPCR analysis. Left ventricular mass was assessed by echocardiography. In addition, to emphasize the beneficial role of AMPK on heart vascular permeability, AMPK activator (acadesine) was administered to C57Bl6 mice before LPS injection. The ANOVA test with Bonferroni's post hoc test and the log-rank test were used. P < 0.05 was considered as significant. Increased cardiac vascular permeability was observed in the LPS group in comparison to untreated animals (2.5% vs. 16%; P < 0.05). The α1KO mice exhibited an increase vascular permeability after LPS injection in comparison to wild-type mice (41.5% vs. 16%; P < 0.05). α1KO animals had a significant mortality increase after LPS injection (70% vs. 10%; P < 0.05). LPS markedly induced the production of proinflammatory cytokines (TNFα, IL-1β, IL-6) that were significantly higher in the α1KO animals. More importantly, LPS treatment leads to an increased left ventricular mass in the α1KO mice within 24 hours, suggesting the onset of edema. Finally LPS-induced vascular hyperpermeability was greatly reduced after AMPK activation by acadesine (13.2% vs. 40%; P < 0.05). AMPK importantly regulates cardiac vascular permeability and could control the sepsis-induced cardiomyopathy. AMPK could represent a new pharmacological target of sepsis. [less ▲]

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See detailIndirect comparison of bazedoxifene vs. oral bisphosphonates for the prevention of nonvertebral fractures in high-risk postmenopausal osteoporosis population
Ellis, A; Jansen, J; Luo, X et al

in Osteoporosis International (2012, March), 23(S2), 64-65

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See detailEffect of collagen hydrolysate in articular pain: A 6-month randomized, double-blind, placebo controlled study
Bruyère, Olivier ULg; Zegels, Brigitte ULg; LEONORI, Lorenzo ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 362-363

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See detailLong-term (3 years) reproducibility for the radiological assessment of knee osteoarthritis
DEROISY, Rita ULg; Bruyère, Olivier ULg; Gensburger, Deborah et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 219-220

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See detailCost-effectiveness of bazedoxifene compared with raloxifene in the treatment of postmenopausal osteoporotic women
Hiligsmann, Mickaël ULg; Ben Sedrine, Wafa ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 312-313

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See detailRadiological and clinical profil of osteoarthritic patients undergoing of total joint replacement
Neuprez, Audrey ULg; François, Garance ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 129-130

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See detailAssessment of joint space narrowing in knee osteoarthritis has good long-term intercentre reproducibility when read in pairs with a semi-automated device
Gensburger, Deborah; DEROISY, Rita ULg; Arlot, Monique et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 247-248

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See detailPerception, knowledge and use by general practitioners of Belgium of the FRAX tool
Bruyère, Olivier ULg; Nicolet, Delphine ULg; Compère, Stéphanie et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 363-364

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See detailAn economic evaluation of strontium ranelate for the treatment of male osteoporosis
Hiligsmann, Mickaël ULg; Ben Sedrine, Wafa ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 305-306

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See detailSevere prevalent vertebral fractures predict subsequent vertebral and nonvertebral fractures: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 361-362

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See detailSeverity of incident vertebral fracture and future fracture risk: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 60-61

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See detailDifference between total and intact assays for N-terminal propeptide of type I procollagen in renal impaired patients
CAVALIER, Etienne ULg; CARLISI, Ignazia ULg; ROUSSELLE, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Supplement 2), 339

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See detailEstablishment of parathyroid hormone (PTH) reference on 10 different assay kits: impact of the recruitment of the population
CAVALIER, Etienne ULg; SOUBERBIELLE, Jean-Claude

in Osteoporosis International (2012, March), 23(Supplement 2), 360

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See detailStructure modifying effects of strontium ranelate in knee osteoarthritis
Reginster, Jean-Yves ULg; Chapurlat, R; Christiaensen, C et al

in Osteoporosis International (2012, March), 23(S2), 58-59

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See detailStrontium ranelate uncouples bone formation from bone resorption in male osteoporotic patients
COLLETTE, Julien ULg; Reginster, Jean-Yves ULg

in Osteoporosis International (2012, March), 23(S2), 365-366

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See detailStrontium ranelate and risk of venous thromboembolism (VTE) : an update of a retrospective cohort study in the UK general practice research database (GPRD)
Cooper, C; Deltour, N; Speirs, C et al

in Osteoporosis International (2012, March), 23(S2), 364-365

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See detailEstimation of genetic parameters for methane indicator traits based on milk fatty acids in dual purpose Belgian blue cattle
Kandel, Purna Bhadra ULg; Soyeurt, Hélène ULg; Gengler, Nicolas ULg

in Communications in Agricultural and Applied Biological Sciences (2012, February 10), 77(1), 21-25

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid ... [more ▼]

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid based CH4 indicators published in the literature were predicted from milk mid-infrared spectra using 597 calibration samples. For the indicator showing the highest link (R2 =0.88) with SF6 CH4 data, the average daily heritability was 0.21, 0.20 and 0.10 for each lactation, respectively. The sire genetic variability was on average 2.82 kg2 of CH4 per lactation. The genetic difference between the sires having cows eructing higher and lower CH4 was 10 kg of CH4 averaged per lactation. In conclusion, CH4 indicators can be predicted by MIR and the genetic variability of these traits seems to exist. [less ▲]

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