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See detailAssessment of quality of life in patients undergoing total joint replacement for OA of the lower limb
Neuprez, Audrey ULg; François, Garance ULg; Bruyère, Olivier ULg et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 693

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See detailChildren of lesbian parents: Research on family triadic interactions
Miscioscia, Marina ULg; Scarciotta, Lidia; D'Amore, Salvatore ULg et al

in Infant Mental Health Journal (2012, June), 33(3 Abstract Supplement), 26

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See detailPerception, knowledge and use by general practitioners of Belgium of the FRAX tool
Bruyère, Olivier ULg; Nicolet, Delphine ULg; Compère, Stéphanie et al

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 716

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See detailEffect of mechanical weeding on wild Chamomile populations in winter wheat crop
Jaunard, Delphine ULg; Monty, Arnaud ULg; Henriet, François et al

in Communications in Agricultural and Applied Biological Sciences (2012, May 22)

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See detailAutomatic real-time collection of RCS of airplanes in a real bistatic low-frequency configuration using a software defined passive radar based on illuminators of opportunity
Pisane, Jonathan ULg; Azarian, Sylvain; Lesturgie, Marc et al

in Proceedings of the 2012 IEEE Radar Conference (2012, May)

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See detailEFFECT OF PARECOXIB, A SELECTIVE COX-2 INHIBITOR, IN THE PREVENTION OF POSTOPERATIVE ADHESIONS IN A RAT MODEL
Arung Kalau, Willy ULg; Drion, Pierre ULg; CHERAMY-BIEN, Jean-Paul ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8444

Objectives: Postoperative peritoneal adhesions are frequent after abdominal surgery. Many preventive agents have been tried in animal models and in clinical trials, but up to now, there has been no ... [more ▼]

Objectives: Postoperative peritoneal adhesions are frequent after abdominal surgery. Many preventive agents have been tried in animal models and in clinical trials, but up to now, there has been no definitive strategy to prevent their formation. In this study, the effectiveness of parecoxib (Dynastat®), a selective cyclooxygenase-2 inhibitor, in preventing experimental intra-abdominal adhesions in rats was studied. Methods: Thirty male rats who underwent a primary surgical procedure aiming at inducing peritoneal injury to produce intraabdominal adhesion, were randomized in three groups: (A) control group, no therapy; (B) intraperitoneal (IP) parecoxib group; (C) intramuscular (IM) parecoxib group. Ten days later, a xyphopubic midline incision was performed and the whole abdominal cavity was explored to score the peritoneal adhesions. Results: Twenty-three rats developed adhesions, 9 (100%) in group A, 7 (70%) in group B and 4 (40%) in group C (P=0.01). The extent and severity scores of adhesion were significantly lower in groups B and C than those in control group (p<0.001). Type of adhesions was measured at 2.25 ± 0.67 in group A, 1.20 ± 0.86 in group B and 0.82 ± 0.80 in group C. This was significantly different between group A and B (p<0.001), between A and C (p<0.001). But no significant difference was found between group B and C (p=0.17). Conclusions: In this study, we found a significant effect on parecoxib in the prevention of postoperative adhesions. But, without avoiding completely the formation of adhesion, parecoxib reduces significantly extent and severity of postoperative adhesions in rats treated with IP or IM parecoxib administration. [less ▲]

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See detailResults of kidney transplantation from controlled donors after cardio-circulatory death: a single center experience
Le Dinh, Hieu ULg; WEEKERS, Laurent ULg; BONVOISIN, Catherine ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 667

Objectives: The aim of this study was to determine results of kidney transplantation (KT) from controlled donation after cardio-circulatory death (DCD). Primary end-points were graft and patient survival ... [more ▼]

Objectives: The aim of this study was to determine results of kidney transplantation (KT) from controlled donation after cardio-circulatory death (DCD). Primary end-points were graft and patient survival, and post-transplant complications. The influence of delayed graft function (DGF) on graft survival and DGF risk factors were analyzed as secondary end-points. Methods: This is a retrospective mono-center review of a consecutive series of 80 DCD-KT performed at the University Hospital of Sart Tilman, University of Liège, between Jan 2005 and Dec 2011. Mean patient follow-up was 28.5 months. Results: Overall graft survival was 93.7%, 89.5%, 85% and 81.3% at 3 months, 1 year, 3 and 5 years, respectively. Death-censored graft survival at the corresponding time points was 93.7%, 93.7%, 90.8% and 90.8%. Main cause of graft loss was patient’s death with a functioning graft. No primary non-function grafts were encountered. Renal graft function was suboptimal at hospital discharge, but nearly normalized at 3 months. DGF was observed in 36% of all DCD-KT. DGF significantly increased post-operative length of hospitalization, but had no deleterious impact on graft function or survival. Donor body mass index (BMI) ≥30 kg/m2, recipient BMI ≥30 kg/m2 and pre-transplant dialysis duration significantly increased the risk of DGF in a multivariate logistic regression analysis (p < 0.05). Conclusions: Despite a higher rate of DGF, controlled DCD-KT offers a valuable contribution to the pool of deceased donor kidney grafts, with comparable mid-term results to those procured after brain death. [less ▲]

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See detailDREAM 2012: DEVELOPMENT OF LAPAROSCOPIC SURGERY AT THE UNIVERSITY HOSPITAL OF KINSHASA, DRC
Nsadi Fwene, Berthier ULg; Veyi, D; Kazadi, J et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8240

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that ... [more ▼]

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that laparoscopy may be developed in the Cliniques Universitaires de Kinshasa (CUK), and may be cost-effective. The final aim of this program is to bring the benefits of laparoscopy to the DRC population, by allowance of adequate training on the UNIKIN personnel, including anaesthetists, surgeons and nurses, who in the future will have to locally form the DRC medical and nursery students. Methods: With the financial support from Wallonie-Bruxelles International (WBI), a complete CUK team, including a surgeon (2 years training in Belgium), an anaesthetist and nurses, were trained in Belgium and then afterwards in DRC. The laparoscopic equipment was sent to Kinshasa, and three theoretical and practical missions of Belgian teams were organised. Results: Over a 2 year period, 116 laparoscopic procedure were performed, including 32 appendectomies, 41 cholecystectomies, 11 hernia repairs, 9 laparoscopy explorations for peritoneal carcinoma assessment and biopsy, 8 procedures for catheter of dialysis peritoneal, 5 gynecologics procedures, and 10 other miscellaneous procedures. Conclusions: A joined approach, taking into account on one hand the training of the skills locally trained to adapt itself to some difficulties, on the other hand institutions of scientific support and a real program and local will of development of this new procedure are the wages of development, accessibility and durability of such news approach in developing countries. All University and non-University team willing to join such a project are welcome. [less ▲]

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See detailEfficacy of a strontium ranelate 2 G/vitamin D3 1000 UI combination on the correction of vitamin D insufficiency
Rizzoli, R; Dawson-Hughes, B; Kaufman, JM et al

in Osteoporosis International (2012, May), 23(S2), 225

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See detailWhat is the potential increase of the heart graft pool by cardiac donation after circulatory death?
NOTERDAEME, Timothée; HANS, Marie-France ULg; NELLESSEN, Eric ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 668

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See detailLaparoscopic liver resection: a single center experience
SZECEL, Delphine ULg; DE ROOVER, Arnaud ULg; DELWAIDE, Jean ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 631

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See detailLiège experience in donation after cardiac death liver transplantation: 2003-2011
Le Dinh, Hieu ULg; DELWAIDE, Jean ULg; MONARD, Josée ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 6811

Objectives: Results of DCD-LT at the University Hospital of Liège were evaluated from 2003 to 2011. Methods: Medical records of 56 DCD liver recipients were retrospectively reviewed with regard to patient ... [more ▼]

Objectives: Results of DCD-LT at the University Hospital of Liège were evaluated from 2003 to 2011. Methods: Medical records of 56 DCD liver recipients were retrospectively reviewed with regard to patient and graft survivals and biliary complications. Mean follow-up was 26.4 months. Mean donor age was 56.3±14.5 years (25 - 83). Donor causes of death were due to anoxia (51.8%), stroke (32.1%) and head trauma (14.3%). Mean WIT, CIT and suture time were 20.5±7.1min (10 – 39), 265.6±85.1min (105 – 576), and 40.8±7.8 min (25 – 61), respectively. 95% of liver grafts were locally shared. HTK was the most commonly used perfusion solution (86%). Mean recipient age was 56.6±10.5 years (29 – 73). Indications for LT included ESLD (53.6%) and HCC (46.6%). Mean MELD score at transplant was 15.6±6.1points (6 – 40). Results: No primary non-function grafts. Mean peak serum AST and bilirubin levels were 2520±3621UI/L and 50.2±49.2mg/L, respectively. Eight patients (14.3%) developed biliary complications. No intra-hepatic bile duct strictures or re-transplantation. Global patient and graft survival was 92.6% at 3 months, 92.6% at 1 year, 73.8% at 3 years and 60% at 5 years. Death-censored patient and graft survival at the corresponding time points was 92.6%, 92.6%, 87.7% and 87.7%. Thirteen liver grafts were lost during follow-up exclusively due to recipient deaths. The rate of HCC recurrence was 33.3%. Conclusions: Controlled DCD donors are a valuable source of transplantable liver grafts. Primary results are encouraging and apparently as good as those from brain-dead donation LT essentially due to short WIT and CIT. [less ▲]

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See detailINTRAPERITONEAL ADHESIONS AFTER OPEN OR LAPAROSCOPIC ABDOMINAL PROCEDURE: AN EXPERIMENTAL STUDY IN THE RAT
Arung, Willy; Drion, Pierre ULg; CHERAMY-BIEN, Jean-Paul ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8546

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 ... [more ▼]

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 pneumoperitoneum is also largely discussed. This study aimed to compare adhesion formation following peritoneal injury by electrocoagulation performed through open or laparoscopic procedures in a rat model. Methods: Sixty male rats were randomized to undergo a 1.5cm peritoneal injury with unipolar cautery under general anesthesia: open surgery (group A, n=20), laparoscopic surgery with CO2 pneumoperitoneum (group B, n=20) and laparoscopic surgery with air pneumoperitoneum (group C, n=20). Duration of the procedures was fixed at 90 minutes in all groups, and pneumoperitoneum pressure at 10mmHg. Ten days later, the animals underwent a secondary laparotomy to score peritoneal adhesions using qualitative and quantitative parameters. Results: Forty-five rats developed at least one adhesion, respectively 95% in group A, 83% in group B and 55% in group C (P<0.01; Group C vs Group A, P<0.01). According to number, thickness, tenacity, vascularization, extent, type, and grading according to Zühkle classification, no significant difference was observed between groups A and B. The distribution of adhesions after open surgery was significantly different than after laparoscopic surgery (P<0.001). Interestingly, group C rats developed significantly less adhesions at the traumatized site, and their adhesions had less severe qualitative scores compared to open surgery (P<0.01). Conclusions: In this animal model, CO2 laparoscopic surgery did not decrease the formation of postoperative adhesion, compared to open surgery. The difference with the animals operated with air pneumoperitoneum emphasizes the role of CO2 in peritoneal injury leading to adhesion formation. [less ▲]

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See detailINTRA-TUMORAL HETEROGENEITY AND RATIONAL SELECTION OF ANTIGENS FOR TARGETED THERAPY OF LIVER METASTASES
Turtoi, Andrei ULg; Blomme, Arnaud ULg; Delvaux, David ULg et al

in Acta Chirurgica Belgica (2012, May), 112(3), 8953

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer ... [more ▼]

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer cells causing treatment resistance and relapse. Therefore, a rational strategy is needed to select targetable antigens that would overcome this intra-tumoral heterogeneity. Methods: After ethical committee approval, 48 fresh liver metastases of colorectal origin were prospectively collected from patients undergoing liver resection. Here we macroscopically divided the lesion in different zones and generated a unique quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. Particular focus was laid on accessible proteins, a protein subclass comprising cell membrane associated and extracellular proteins. Accordingly, the tissues were ex-vivo biotinylated, affinity purified and analyzed for each zone separately using nano-UPLC-MSe proteomics technique. In total over 1500 unique proteins were statistically divided into different patterns of expression. Results: We have generated a quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. The study offers insight into novel targets but also antigens against which the antibodies are already involved in clinical trials or treatment of liver metastases. Extensive clustering and validation experiments highlight novel markers that offer the potential to homogeneously cover the metastatic lesion and become better targets. Conclusions: Two such antigens, LTBP2 and TGFBI were selected for functional analysis in colorectal carcinoma cells. In vitro and in vivo experiments showed that in particular TGFBI is relevant for migration and proliferation capacity of colorectal cancer cells. The suppression of this protein led to significant inhibition of tumor growth, crystalizing it as bona fide target for the development of anti-metastases therapies. [less ▲]

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See detailFeasibility of scanning fetal anatomy in the first trimester of gestation
To, Hong ULg

in European Federation of Societies for Ultrasound in Medicine and Biology (2012, April 22)

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and ... [more ▼]

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and methods: In a prospective study, 2500 singleton pregnancies measured fetal NT and scanned structural anatomy in the first trimester; then checked fetal morphology systematically at 18-24 weeks and followed up to their delivery. According to ultrasonographic abnormalities and amniocentesis, we evaluated the detection rate of ultrasound for aneuploidy in the first and second trimester of gestation. Results: The sensitivity and specificity of ultrasound for aneuploidy was 17.2% and 99.7%, respectively. All 13 cases of fetal ultrasonographic anomalies related significantly with an increased NT (≥ 2.4mm) (p < 0.001). 30.8% of these abnormalities that included cystic hygroma, omphalocele and holoprosencephaly were detected at 11-13.6 weeks’ gestation. In the second trimester scanning, 69.2% of major structural anomalies found and the detection rate of central nervous system malformation, abnormal heart, abdominal wall defect, face anomaly and skeletal deformities were 85.7%, 100%, 66.7%, 50% and 75%, alternatively. Conclusion: Scanning of fetal anatomy at the time of NT measuring is useful to detect fetal abnormalities, especially aneuploidy in Vietnamese pregnancies. [less ▲]

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See detailRisk factor of fetal chromosomal aberrations in the south of Vietnam
To, Hong ULg

in European Federation of Societies for Ultrasound in Medicine and Biology (2012, April 22)

Objectives: The aim of this study is to find the risk factors of fetal chromosomal aberrations in order to classify a high risk pregnancy in our prenatal care unit. Material and methods: A prospective ... [more ▼]

Objectives: The aim of this study is to find the risk factors of fetal chromosomal aberrations in order to classify a high risk pregnancy in our prenatal care unit. Material and methods: A prospective study was carried out during one year in our clinic. 523 cases of amniocentesis was followed up and evaluated the relation with abnormal result (fetal aneuploidies) and the risk factors such as advanced maternal age (≥ 35 years old), parity, habitation, positive triple test (≥ 1/250), fetal malformation history and abnormal signs in the first and second trimester scanning. Results: The incidence of fetal aneuploidy was 3.4% (18/523 [95%CI: 2.1-5.4%]) and the trisomy 21 showed the most common in these chromosomal aberrations (67%). The younger women (< 35 years old), nulliparous, positive triple test and abnormal ultrasonographic findings (included increased nuchal translucency [≥ 2.4mm], absent nasal bone in the first trimester scanning and/or echogenic nodule in ventricular) was identified as the risk factors of fetal aneuploidies (OR was 2.3 [95%CI: 0.8-6.7], 4.0 [95%CI: 1.4-13.2], 3.3 [95%CI: 0.3-33.5] and 9.4 [95%CI: 1.1-79.2], respectively). The abnormal ultrasonographic findings found a relation significantly with abnormal chromosomes (p=0.04). Conclusion: Abnormal ultrasonographic findings in the first and second trimester were the important markers for predicting fetal aneuploidy, especially trisomy 21. [less ▲]

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See detailLa bande dessinée belge avant Hergé. Quelle histoire ?
Paques, Frédéric ULg

in Comicalités : Études de Culture Graphique (2012, April 11)

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See detailExpression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates
Lambert, Cécile ULg; Dubuc, Jean-Emile; Hennuy, Benoît ULg et al

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

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See detailA prospective study of cardiac valvular status in patients treated with cabergoline for Endocrine Disease
VROONEN, Laurent ULg; Lancellotti, Patrizio ULg; Tome Garcia, M et al

in Annales d'Endocrinologie (2012, April), 73(2), 148

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See detailTHE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION
Shafiei, Rasoul ULg; Thonart, Philippe ULg

in Acetic Acid Bacteria (2012, April)

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular ... [more ▼]

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular functions of acetic acid bacteria. In previous studies in CWBI, Acetobacter senegalensis was used for production of dried vinegar starters; however the impact of stressors (ethanol and acetic acid) on A. senegalensis remained unclear. In this study, different techniques such as flow cytometry, culturability on solid medium and 2-DiGE were used comparatively to investigate the effect of carbon sources of inoculum media on the tolerance of A. senegalensis to stressors. Analysis of respiration system by flow cytometric methods showed that the presence of 2% (v/v) acetic acid in inoculum medium, in one hand, causes 80% of cells to continue to do respiration after a sudden exposure to 1- 3% (v/v) acetic acid in stress media while 89.7% of cells grown in glucose appeared as dead cells after an abrupt exposure to 3%(v/v) of acetic acid. On the other hand, 59.2% and 49.33% of cells grown in the presence of 2% (v/v) of acetic acid could maintain their entire membrane integrity after exposure to 1% and 3% (v/v) of acetic acid, respectively. Inoculum medium contained 5% (v/v) of ethanol as a carbon source enabled about 90% of cells to keep their growing capacities after a sudden exposure to 3% acetic acid. In contrast, just 40% of cells grown in glucose as a carbon source maintained their culturability on solid medium after exposure to 1% acetic acid. A similar profile of culturability was observed for the cells grown in 5% (v/v) ethanol or 2% (v/v) of acetic acid. A proteomic approach (2-DiGE) was used to analyze proteins expressed in the presence of different carbon sources. Differentially expressed proteins were mainly associated with energy metabolism, carbohydrate metabolisms, folding, sorting and degradation processes. The relative abundance of proteins was extensively different for cell grown in glucose compared with protein contents of cells grown in ethanol or acetic acid. In conclusion, production of a cost effective vinegar starter needs a qualified biomass which tolerates ethanol and acetic acid. Tolerance of A. senegalensis to acetic acid depends to a great extent on the composition of the medium which cells grow in. In spite of low adaption to acetic acid for cell grown in glucose, using ethanol or acetic acid in inoculum media renders a physiological state in A. senegelensis which enables it to cope with higher concentration of acetic acid readily, this biomass has a potential to be used as a starter [less ▲]

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See detailClinical characterization of cabergoline resistant prolactinomas : a multicenter experience on 92 patients
VROONEN, Laurent ULg; Jaffrain Rea, ML; PETROSSIANS, Patrick ULg et al

in Annales d'Endocrinologie (2012, April), 73(2), 153

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See detailExpression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates
Lambert, Cécile ULg; Dubuc, Jean-Emile; Hennuy, Benoît ULg et al

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

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See detailEvaluation of three immunoassays for serodiagnosis of human Mycoplasma pneumoniae infection
HUYNEN, Pascale ULg; TOUSSAINT, Françoise ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2012, April), 18(S3), 231

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See detailHumidity 3D field comparisons between GNSS tomography, IASI satellite observations and ALARO model
Brenot, Hugues; Champollion, Cedric; Deckmyn, Alex et al

in Geophysical Research Abstracts (2012, April), 14

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See detailAMICE: erste internationale Modellrechnung der Maas
Becker, Bernhard; Patzke, Simone; Detrembleur, Sylvain ULg et al

in Forum für Hydrologie und Wasserbewirtschaftung (2012, March 22), 12(31), 165-170

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den ... [more ▼]

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den Anrainerstaaten Frankreich, Belgien, Deutschland und der Niederlande vorgehaltenen numerischen Modelle verwendet. Die Randbedingungen dieser Modelle wurden in einem iterativen Prozess abgeglichen. Die Ergebnisse der zusammenhängenden Simulation tragen zunächst zu einem besseren Systemverständnis bei. Im weiteren Verlauf des Projektes fließen sie in eine Risikobetrachtung ein und werden zur Bewertung von Maßnahmen zur Reduzierung des Hochwasserrisikos vor dem Hintergrund zu erwartender Änderung der Bemessungsgrößen infolge einer erwarteten Klimaänderung herangezogen. [less ▲]

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See detailLA FALSIFICATION DES MEDICAMENTS
Marini Djang'Eing'A, Roland ULg

in Forum Pharmaceutique (2012, March 16), -(-), 16

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See detailHigh-resolution spectropolarimetric observations of hot subdwarfs
Petit, P.; Van Grootel, Valérie ULg; Bagnulo, S. et al

in Leaflet - Astronomical Society of the Pacific (2012, March 01), 452

We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I ... [more ▼]

We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I and V line profiles to enhance the signal-to-noise ratio. We then average all available cross-correlation profiles of each star to further decrease the noise level. Although both targets were previously reported to host kilo-gauss magnetic fields, we do not derive any evidence of large-scale photospheric fields from our sets of observations, in spite of tight error bars on the longitudinal field of the order of 60 gauss for HD 76431 and 200 gauss for Feige 66. A new analysis of FORS1 observations of HD 76431, which provided the basis for the original claim of field detection, confirms the absence of any detectable Zeeman signature, with an error bar of about 100 gauss on the longitudinal magnetic field. [less ▲]

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See detailCost-effectiveness of bazedoxifene compared with raloxifene in the treatment of postmenopausal osteoporotic women
Hiligsmann, Mickaël ULg; Ben Sedrine, Wafa ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 312-313

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See detailAMP-activated protein kinase controls liposaccharide-induced hyperpermeability
Castanares-Zapatero, Diego; Overtus, M; Communi, Didier et al

in Critical Care (2012, March), 16(suppl 1), 17

Organ dysfunction determines the severity of sepsis and is correlated to mortality. Endothelial increased permeability contributes to the development of organ failure. AMP-activated protein kinase (AMPK ... [more ▼]

Organ dysfunction determines the severity of sepsis and is correlated to mortality. Endothelial increased permeability contributes to the development of organ failure. AMP-activated protein kinase (AMPK) has been shown to modulate cytoskeleton and could mediate endothelial permeability. Our hypothesis is that AMPK controls sepsis-induced hyperpermeability in the heart and is involved in septic cardiomyopathy. Sepsis was induced by intraperitoneal injection of liposaccharide, 10 mg/kg (LPS). Alpha-1 AMPK knockout mice (α1KO) were compared with wild-type. Vascular permeability was characterized by Evans blue extravasation. Inflammatory cytokine mRNA expression was determined by qPCR analysis. Left ventricular mass was assessed by echocardiography. In addition, to emphasize the beneficial role of AMPK on heart vascular permeability, AMPK activator (acadesine) was administered to C57Bl6 mice before LPS injection. The ANOVA test with Bonferroni's post hoc test and the log-rank test were used. P < 0.05 was considered as significant. Increased cardiac vascular permeability was observed in the LPS group in comparison to untreated animals (2.5% vs. 16%; P < 0.05). The α1KO mice exhibited an increase vascular permeability after LPS injection in comparison to wild-type mice (41.5% vs. 16%; P < 0.05). α1KO animals had a significant mortality increase after LPS injection (70% vs. 10%; P < 0.05). LPS markedly induced the production of proinflammatory cytokines (TNFα, IL-1β, IL-6) that were significantly higher in the α1KO animals. More importantly, LPS treatment leads to an increased left ventricular mass in the α1KO mice within 24 hours, suggesting the onset of edema. Finally LPS-induced vascular hyperpermeability was greatly reduced after AMPK activation by acadesine (13.2% vs. 40%; P < 0.05). AMPK importantly regulates cardiac vascular permeability and could control the sepsis-induced cardiomyopathy. AMPK could represent a new pharmacological target of sepsis. [less ▲]

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See detailAn economic evaluation of strontium ranelate for the treatment of male osteoporosis
Hiligsmann, Mickaël ULg; Ben Sedrine, Wafa ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 305-306

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See detailLong-term (3 years) reproducibility for the radiological assessment of knee osteoarthritis
DEROISY, Rita ULg; Bruyère, Olivier ULg; Gensburger, Deborah et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 219-220

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See detailRadiological and clinical profil of osteoarthritic patients undergoing of total joint replacement
Neuprez, Audrey ULg; François, Garance ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 129-130

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See detailAssessment of joint space narrowing in knee osteoarthritis has good long-term intercentre reproducibility when read in pairs with a semi-automated device
Gensburger, Deborah; DEROISY, Rita ULg; Arlot, Monique et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 247-248

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See detailSevere prevalent vertebral fractures predict subsequent vertebral and nonvertebral fractures: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 361-362

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See detailEffect of collagen hydrolysate in articular pain: A 6-month randomized, double-blind, placebo controlled study
Bruyère, Olivier ULg; Zegels, Brigitte ULg; LEONORI, Lorenzo ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 362-363

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See detailPerception, knowledge and use by general practitioners of Belgium of the FRAX tool
Bruyère, Olivier ULg; Nicolet, Delphine ULg; Compère, Stéphanie et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 363-364

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See detailEfficacy of once-yearly zoledronic acid 5 mg in men with osteoporosis with different levels of serum total testosterone
Boonen, S; Reginster, Jean-Yves ULg; Kaufman, JM et al

in Osteoporosis International (2012, March), 23(S2), 79-80

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See detailAutomata-Based Symbolic Representations of Polyhedra
Boigelot, Bernard ULg; Brusten, Julien ULg; Degbomont, Jean-François ULg

in Lecture Notes in Computer Science (2012, March), 7183

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See detailStructure modifying effects of strontium ranelate in knee osteoarthritis
Reginster, Jean-Yves ULg; Chapurlat, R; Christiaensen, C et al

in Osteoporosis International (2012, March), 23(S2), 58-59

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See detailDenosumab treatment of postmenopausal women with osteoporosis for 6 years : results from the first 3 years of the freedom extension
Papapoulos, S; Brown, JP; Chapurlat, R et al

in Osteoporosis International (2012, March), 23(S2), 76

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See detailStrontium ranelate uncouples bone formation from bone resorption in male osteoporotic patients
COLLETTE, Julien ULg; Reginster, Jean-Yves ULg

in Osteoporosis International (2012, March), 23(S2), 365-366

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See detailStrontium ranelate reduces the number of radiological or radioclinical progressors in patients with primary knee osteoarthritis
Reginster, Jean-Yves ULg; Chapurlat, R; Christiansen, C et al

in Osteoporosis International (2012, March), 23(S2), 366-367

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See detailEstablishment of parathyroid hormone (PTH) reference on 10 different assay kits: impact of the recruitment of the population
CAVALIER, Etienne ULg; SOUBERBIELLE, Jean-Claude

in Osteoporosis International (2012, March), 23(Supplement 2), 360

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See detailStrontium ranelate and risk of venous thromboembolism (VTE) : an update of a retrospective cohort study in the UK general practice research database (GPRD)
Cooper, C; Deltour, N; Speirs, C et al

in Osteoporosis International (2012, March), 23(S2), 364-365

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See detailDifference between total and intact assays for N-terminal propeptide of type I procollagen in renal impaired patients
CAVALIER, Etienne ULg; CARLISI, Ignazia ULg; ROUSSELLE, Olivier ULg et al

in Osteoporosis International (2012, March), 23(Supplement 2), 339

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See detailWhat are the most important medication attributes for patients with osteoporosis ? Results from a qualitative study
Hiligsmann, Mickaël ULg; Van Durme, C; Geusens, P et al

in Osteoporosis International (2012, March), 23(S2), 81-82

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See detailIndirect comparison of bazedoxifene vs. oral bisphosphonates for the prevention of nonvertebral fractures in high-risk postmenopausal osteoporosis population
Ellis, A; Jansen, J; Luo, X et al

in Osteoporosis International (2012, March), 23(S2), 64-65

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See detailSeverity of incident vertebral fracture and future fracture risk: a 3-year prospective study
Bruyère, Olivier ULg; Roux, Christian; Nicolet, Delphine ULg et al

in Osteoporosis International (2012, March), 23(Suppl. 2), 60-61

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See detailEstimation of genetic parameters for methane indicator traits based on milk fatty acids in dual purpose Belgian blue cattle
Kandel, Purna Bhadra ULg; Soyeurt, Hélène ULg; Gengler, Nicolas ULg

in Communications in Agricultural and Applied Biological Sciences (2012, February 10), 77(1), 21-25

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid ... [more ▼]

The genetic parameters of CH4 indicators were estimated by single trait test-day models from 16,825 records collected on Walloon Dual Purpose Belgium Blue cows in their first 3 lactations. Fatty acid based CH4 indicators published in the literature were predicted from milk mid-infrared spectra using 597 calibration samples. For the indicator showing the highest link (R2 =0.88) with SF6 CH4 data, the average daily heritability was 0.21, 0.20 and 0.10 for each lactation, respectively. The sire genetic variability was on average 2.82 kg2 of CH4 per lactation. The genetic difference between the sires having cows eructing higher and lower CH4 was 10 kg of CH4 averaged per lactation. In conclusion, CH4 indicators can be predicted by MIR and the genetic variability of these traits seems to exist. [less ▲]

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See detailApport des troponines T et I ultrasensibles dans le diabète
LE GOFF, Caroline ULg; LAURENT, Terry ULg; GARWEG, Christophe ULg et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2012, February), 27(1), 40

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations ... [more ▼]

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations hautement sensibles pourraient être un bon outil diagnostic pour mettre en évidence des macroou micro-angiopathies non diagnostiquée chez ces patients. Le but de notre étude était de comparer la performance de la troponine T ultrasensible (hsTnT) (Roche Diagnostic) avec la troponine I ultrasensible (TnI II) (Abbott Diagnostic) chez le sujet diabétique. Matériel et méthodes : Vingt patients diabétiques (âge moyen : 52.6 ± 8.4 ans) ont été sélectionnés sur le critère d’un taux d’hémoglobine glyquée (HbA1c) élevé. Ces sujets ont été comparés à vingt sujets contrôles d’âge moyen : 60.05 ± 2.86 ans. Les patients atteints d’insuffisance rénale et d’affections cardiovasculaires ont été exclus. Les analyses ont été réalisées sur du plasma hépariné lithium. La hsTnT a été dosée par électrochemiluminescence sur le Modular E (Roche Diagnostic). Abbott utilise des microparticules chemiluminescentes pour le dosage de la TnI II sur l’ARCHITECT i. Résultats : Les sujets diabétiques ont un taux plasmatique de hsTnT représenté par les valeurs suivantes [médiane (1er quartile, 3ème quartile)] : 0,007 (0,03 ; 0,018) ng/mL ; alors que les sujets contrôles négatifs présentent les valeurs suivantes : 0,003 (0,003 ; 0,004) ng/mL. La différence de taux de hsTnT observée entre ces 2 populations est statistiquement significative (p = 0,000922). Pour la TnI II, on observe un taux plasmatique de: 0,004 (0,003 ; 0,075) ng/mL chez les sujets diabétiques et 0,002 (0,001 ; 0,005) ng/mL chez les sujets contrôles. La différence de taux de TnI II observée entre ces 2 populations n’est pas statistiquement significative (p > 0,005). Discussion-conclusion : Au décours d’un diabète, il apparait que les taux de hsTnT sont augmentés de façon significative par rapport au groupe des contrôles négatifs. Si les taux hsTnT augmentent chez le diabétique, il semblerait normal qu’il en soit de même pour la TnI II, vu que ces deux marqueurs sont intimement liés. Nous ne l’avons pas observé mais ceci peut être du à la plus faible performance analytique du dosage de la TnI II utilisé. Cette libération de hsTnT dans le sang peut être due aux micro- et macro-angiopathies au niveau des coronaires induites au cours d’un diabète. [less ▲]

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See detailA candidate cell of origin for cervical cancer
Herfs, Michael ULg; Yamamoto; Laury et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (2012, February), 25(2), 4-552

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See detailDynamic Prediction of Energy Delivery Capacity of Power Networks : Unlocking the Value of Real-Time Measurements.
Schell, Peter; Jones, Lauren; Mack, Philippe et al

in Proceedings of the third IEEE PES Conference on Innovative Smart Grid Technologies (2012, January)

This paper describes recent advances in prediction software using advanced data-mining tools, and shows how this can significantly increase the operational value of the data gathered. These new tools ... [more ▼]

This paper describes recent advances in prediction software using advanced data-mining tools, and shows how this can significantly increase the operational value of the data gathered. These new tools allow for new operational procedures to be defined that increase the usages of the network without decreasing security of supply. We will use the example of dynamic line rating, i.e. determining the thermal rating of an overhead line based on real-time environmental parameters (temperature, wind speed & direction and solar radiation) to highlight how the use of numerical data-mining tools can help us reliably predict future behavior of power networks. [less ▲]

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See detailLa nécessité d'un inventaire du patrimoine culturel immatériel
Lempereur, Françoise ULg

in Géants d'ici et d'ailleurs (2012, January), (6-7), 24-30

Destinée essentiellement aux institutions et groupements qui ont en charge la sauvegarde du patrimoine culturel immatériel, cette présentation rapide justifie l'inventorisation de ce patrimoine, en ... [more ▼]

Destinée essentiellement aux institutions et groupements qui ont en charge la sauvegarde du patrimoine culturel immatériel, cette présentation rapide justifie l'inventorisation de ce patrimoine, en définit les responsabilités et propose une méthodologie de collectage et de mise à disposition publique des contenus [less ▲]

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See detailPotentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale-down reactors, and elaboration of a mini scale-down platform
Brognaux, Alison ULg; Neubauer, Peter; Twizere, Jean-Claude ULg et al

in Communications in Agricultural and Applied Biological Sciences (2012), 77(1), 3-7

Substrate limitation responsive biosensors have been used in order to detect spatial substrate heterogeneities, , inside industrial bioreactors (whole-cell biosensor). Three green fluorescent protein (GFP ... [more ▼]

Substrate limitation responsive biosensors have been used in order to detect spatial substrate heterogeneities, , inside industrial bioreactors (whole-cell biosensor). Three green fluorescent protein (GFP) transcriptional reporters have been chosen in E.coli, i.e. uspA::gfp, csiE::gfp and yciG::gfp. The promoter uspA is induced in response to a variety of stresses whereas the two other promoters, csiE and yciG, are supposed to be more specific in front of a substrate limitation. The responsiveness of these biosensors has been assessed in chemostat reactor. Secondly, the same biosensors have been tested in well-mixed laboratory reactors and in scale-down reactors able to reproduce industrial conditions. Finally, a mini scale-down platform has been proposed as a high throughput tool to investigate rapidly the usefulness of a given microbial biosensor. Local heterogeneities in mini-bioreactor have caused a decrease of GFP expression, as in scale-down reactor. The presence of GFP in supernatants was noticed and this leakage seems to be correlated with the membrane permeability. [less ▲]

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See detailEffects of Strontium ranelate on knee osteoarthritis pain : a responder analysis
Reginster, Jean-Yves ULg; Chapurlat, R; Bellamy, N et al

in Arthritis and Rheumatism (2012), 64(S10), 110

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See detailImproving physical education teachers’ action with overweight students
Cloes, Marc ULg

in Revista Motricidade (2012), 8(S1), 3-4

Nowadays, overweight is one of the most important plagues and many authors believe that the fast increase of this phenomenon during the last decades can be considered as a global epidemic. School has been ... [more ▼]

Nowadays, overweight is one of the most important plagues and many authors believe that the fast increase of this phenomenon during the last decades can be considered as a global epidemic. School has been identified as one of the pillars of the necessary multisectorial approach that must be implemented to fight against this ineworable trend. ... [less ▲]

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See detailMultiscale Computational Modeling of a New Scaffold for Anterior Cruciate Ligament Tissue Engineering
Laurent, Cédric ULg

in Computer Methods in Biomechanics & Biomedical Engineering (2012)

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See detailA short introduction to spallation reactions
Cugnon, Joseph ULg

in Few-Body Systems (2012), 53(1-2), 142-152

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See detailIntegrated prism-free coupled surface plasmon resonance biochemical sensor
Lenaerts, Cedric ULg; Hastanin, Juriy ULg; Pinchemel, Bernard et al

in Proceedings of SPIE (2012), 8424

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See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 106

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See detailDo you sound or look as old as you are? A study of age estimation in young and older adults
Moyse, Evelyne ULg; Beaufort, Aline ULg; Brédart, Serge ULg

in Perception (2012), 41(supplement), 117

Studies on age estimation usually indicated that people are fairly accurate at estimating the age of a person from her/his face or from her/his voice (with an absolute difference of five and ten years ... [more ▼]

Studies on age estimation usually indicated that people are fairly accurate at estimating the age of a person from her/his face or from her/his voice (with an absolute difference of five and ten years respectively) [e.g. Amilon et al., 2000, in: Speaker Classification II. Lectures Notes in Artificial Intelligence, C Müller, Berlin, Springer-Verlag]. However studies showed also that performance depends on the age of participants and the age of stimuli [Rhodes, 2009, Applied Cognitive Psychology, 23, 1-12; Braun, 1996, Forensic Linguistics, 3, 65-73]. The aim of the present study is to compare age estimation performance from faces and voices by using an experimental design in which the age of participants (young vs older), the age of stimuli (young vs older) and the stimulus domain (face vs voice) were crossed. Overall, the age of faces was more accurately estimated than the age of voices. Moreover performance of age estimation was better for young stimuli than for older stimuli. Finally, young participants made smaller absolute errors than older participants. However there is no difference between young and older participants when estimating the age of older stimuli. [less ▲]

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See detailChemical control of Haplodiplosis marginata von Roser (Diptera : Cecidomyiidae)
Censier, Florence ULg; Chavalle, Sandrine; Wittouck, Daniel et al

in Communications in Agricultural and Applied Biological Sciences (2012), 78(4), 667-675

The saddle gall midge, Haplodiplosis marginata (von Roser), has been detected in Belgium since 2010, after several decades without any reporting. It had indeed caused serious damages between 1965 and 1970 ... [more ▼]

The saddle gall midge, Haplodiplosis marginata (von Roser), has been detected in Belgium since 2010, after several decades without any reporting. It had indeed caused serious damages between 1965 and 1970. This insect is a European cereal pest whose larvae feed on stems and engender saddle-shaped depressions, resulting in yield losses. Face with the resurgence of this pest, it was decided to study its spatial distribution and, because serious damages were observed in some regions, to develop effective curative control. To date, chemical protection seems to be the only immediate solution in case of heavy emergences. Experimentation was conducted in a highly infested field (Meetkerke, Belgian Polders), according to a randomized complete blocks arrangement with four replications. Foremost, a lambdacyhalothrin-based insecticide was used to evaluate efficiency of several protection schemes, ranging between one and four spray(s). The large spread of flights observed during the 2011 spring allowed to highlight the effect of treatment date on the attack intensity and also on the galls distribution along the stem, on the different internodes: the lower internodes were protected by the early sprayings, while last sprayings induced reduction of galls number on the upper internodes. Moreover, several insecticides already registrated in cereals against aphids were compared for their efficacy against saddle gall midge. Studied pyrethroids have shown a very good efficacy, ranging between 75 % and 87 %, when applied twice with a 2 weeks interval. To be efficient, insecticide applications must thus be synchronized with the flights and egg-laying periods. Monitoring the phenology of flights is thus essential as part of integrated pest management against saddle gall midge. [less ▲]

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See detailEffet structuro-modulateur du ranélate de strontium dans la gonarthrose : l'étude SEKOIA
Chevalier, X; Chapurlat, R; Cooper, C et al

in Revue du Rhumatisme (2012), 79(S1), 271

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See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 106

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See detailAntioxidant and anti-inflammatory like properties of benzoic acid analogs on the oxidant response of neutrophils: structure/redox potential relationship study.
Franck, Thierry ULg; Mouithys-Mickalad, Ange ULg; Robert, Thierry ULg et al

in Free Radical Biology & Medicine (2012), 53(supplement 1),

We investigated the antioxidant capacity of phenolic acid derivatives by measuring their capacity to prevent ABTS oxidation and evaluating their anti-inflammatory like-properties on the oxidant response ... [more ▼]

We investigated the antioxidant capacity of phenolic acid derivatives by measuring their capacity to prevent ABTS oxidation and evaluating their anti-inflammatory like-properties on the oxidant response of neutrophils, especially on superoxide anion production and the activity of myeloperoxidase (MPO), an oxidant enzyme present and released by the primary granules of neutrophils. The superoxide anion production by PMA-stimulated neutrophils was measured by lucigenin-enhanced chimiluminescence (CL) and the activity of MPO by SIEFED to study the potential interaction of a molecule with the enzyme without interferences due to medium. The antioxidant and anti-inflammatory activities of the phenolic compounds were correlated to their redox potentials measured by voltammetry method, and discussed in relation to their molecular structure. The ability of the phenolic molecules to decrease ABTS oxidation and CL production was inversely correlated to their redox potential increasing as follows: propyl gallate > gallic acid > caffeic acid > 3,4-dihydroxybenzoic acid > ferulic acid > syringic acid > 2,6-dihydroxybenzoic acid > salicylic acid > benzoic acid. The number of hydroxyl groups (3) and their position (catechol) were essential for the efficacy of the molecules as stoichiometric antioxidants or scavengers. On MPO activity, the inhibitory capacity of the molecules was not really correlated with their redox potential and increased as follows: gallic acid > caffeic acid > 2,6-dihydroxybenzoic acid > propyl gallate > ferulic acid = syringic acid > 3,4-dihydroxybenzoic acid = salicylic acid > benzoic acid. The number of OH groups and the elongation of the carboxyl group were essential for the inhibition of MPO activity, probably by facilitating the interaction with the MPO active site or structure. The redox potential measurement seems to be a good technique to select stoichiometric antioxidants, but not anti-catalytic ones. [less ▲]

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See detailAssociation entre l'hypertrophie du chondrocyte et l'angiogenèse du cartilage dans l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 105-106

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See detailStrontium ranelate in knee osteoarthritis trial (SEKOIA) : a structural and symptomatic efficacy
Reginster, Jean-Yves ULg; Chapurlat, R; Christiansen, C et al

in Arthritis and Rheumatism (2012), 64(S10), 681

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See detailBone sialoprotein: a key mediator of the angiogenic activity of hypertrophic osteoarthritic chondrocytes
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Baudouin, Caroline et al

in Osteoarthritis and Cartilage (2012), 20(S), 122-123

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See detailOptimizing nutrition after birth with a unique standardized parenteral nutrition solution may reduce electrolytes anomalies in <1250g infants
SENTERRE, Thibault ULg; rigo, jacques

in Archives of Disease in Childhood (2012), 97(S2), 394

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See detailComparison of Immune Reconstitution after Hematopoietic Stem Cell Transplantation with Flu-TBI versus TLI-ATG Conditioning
Hannon, Muriel ULg; Humblet-Baron, S.; Graux, C. et al

in Haematologica (2012), 97(Supplement 1), 180

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See detailLe rôle du concept de Tendenz dans l'analyse husserlienne de la fondation à l'époque des Recherches logiques
Gyemant, Maria ULg

in Bulletin d'Analyse Phénoménologique (2012), 8(N°1), 183-201

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See detailAppel au peuple/appel du public : décrire la réception comme une adresse
Servais, Christine ULg

in Questions de Communication (2012)

Par rapport à d'autres productions culturelles ou scientifiques, la particularité des médias de masse est qu'ils font quelque chose au peuple en faisant quelque chose du peuple, c'est-à-dire en le ... [more ▼]

Par rapport à d'autres productions culturelles ou scientifiques, la particularité des médias de masse est qu'ils font quelque chose au peuple en faisant quelque chose du peuple, c'est-à-dire en le représentant à lui-même. La construction mass-médiatique du "peuple" a donc une dimension pragmatique que les études de réception doivent intégrer. C'est à quoi s'attache cette communication, notamment à travers la notion d'adresse, issue du travail de J. Derrida, et celle de "partage du sensible" formalisée par Jacques Rancière. [less ▲]

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See detailRespiratory system elastance monitoring during PEEP titration
Chiew, YS; Chase, JG; Shaw, GM et al

in Critical Care (2012), 34 (Suppl 1)

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See detail(18F)FPRGD2 PET/CT imaging of integrin αvβ3 in renal carcinomas : correlation with histopathology.
WITHOFS, Nadia ULg; SIGNOLLE, N.; NZARAMBA, EM. et al

in Journal of Nuclear Medicine (The) (2012), 53(SUPPL), 1647

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See detailCorrection of vitamin D insufficiency with the fixed daily combination strontium ranelate 2 g/vitamin D3 1000 IU over 12 months
Rizzoli, R; Dawson-Hughes, B; Kaufman, JM et al

in Arthritis and Rheumatism (2012), 64(S10), 835

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See detailCampagnes de sensibilisation au dépistage du diabète de type 2 dans les pharmacies. Comparaison de deux approches : glycémie capillaire et grille Findrisc
Böhme, P; Agrinier, N; Badia, M et al

in Diabètes & Métabolism (2012), 38(2), 7

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See detailRapamycin Prevents Experimental Sclerodermatous Chronic Graft-versus-Host Disease in mice
Belle, Ludovic ULg; Binsfeld, Marilène ULg; DUBOIS, Sophie ULg et al

in Belgian Journal of Hematology (2012), Abstracts book(Supplement of 27th General Meeting of the Belgian Hematological Society), 14

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See detailConnectivity within the default mode network is related to working memory performance in young but not elderly healthy adults
Yakushev, Igor; Chételat, Gael; Fischer, Florian et al

in Alzheimer's & Dementia : The Journal of the Alzheimer's Association (2012), 8(4), 81

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See detailEfficacy and safety of strontium ranelate in the treatment of knee osteoarthritis : a randomized, double-blind, placebo-controlled international trial
Cooper, C; Chapurlat, R; Christiansen, C et al

in Annals of the Rheumatic Diseases (2012), 71(3), 693

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See detailSpurrite, tilleyite and associated minerals in the exoskarn zone from Cornet Hill (Metalliferi Massif, Apuseni Mountains, Romania).
Marincea, Stefan ULg; Dumitras, Delia; Calin, Nicolas et al

in Geophysical Research Abstracts (2012), 14

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See detailAn experimental approach for synthesis of Fe-Al-O multiferroic fibrous material
Starbov, N; Starbova, K; Vanderbemden, Philippe ULg et al

in Journal of Physics: Conference Series (2012), 398

Basic principles of the electro-hydrodynamics are applied for synthesis of solid state Fe-Al-O multi-ferroic fibrous material. For that purpose stable blend spinning solutions comprised of a high ... [more ▼]

Basic principles of the electro-hydrodynamics are applied for synthesis of solid state Fe-Al-O multi-ferroic fibrous material. For that purpose stable blend spinning solutions comprised of a high molecular assisting organic polymer and salts of iron and aluminum are developed. These solutions are tested under electrospinning conditions and synthesis of homogeneous as spun non-woven mats characterized by fibre mean diameters in the microand nano-size range is successfully demonstrated. Multi-step thermal procedure is applied for the consecutive solvent evaporation, polymer pyrolisis and final fibre calcination. Electronoptical imaging technique and XRD are applied for revealing the sample morphology and the phase composition correspondingly. The results obtained outline the wide possibilities for fabrication of multi-ferroics fibrous nano-materials on the basis of Fe-Al-O. [less ▲]

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See detailDoes delay release the verbal overshadowing effect in child and adult eyewitnesses?
Vanootighem, Valentine ULg; Brédart, Serge ULg; Dehon, Hedwige ULg

in Perception (2012), 41(supplement), 194

The verbal overshadowing effect (VO) (eg, Schooler and Engstler-Schooler, 1990 Cognitive Psychology 22(1) 36–71) suggests that the fact of generating a verbal description of a previously seen face may ... [more ▼]

The verbal overshadowing effect (VO) (eg, Schooler and Engstler-Schooler, 1990 Cognitive Psychology 22(1) 36–71) suggests that the fact of generating a verbal description of a previously seen face may impair subsequent performance on a lineup identification task in adults. Previous research has examined whether descriptions also impaired children’s identification abilities but no evidence of VO was found (Memon and Rose, 2002 Psychology, Crime and Law 8(3), 229–242). However, the method might not have been appropriate to observe this effect as, for instance, a 24-hour delay between the description and the identification tasks (associated with a release of the VO effect in adults) was used. Hence, in this current experiment, groups of children (7–8, 10–11, 13–14 years old) and adults were presented with a short video and then assigned to a description or a no description condition before the identification task. Participants were also assigned either to a “no delay”, a “24-hour post encoding delay” or a “24-hour post description delay” condition to determine the influence of delay on the VO effect. Results indicated that, compared to the control condition, the description decreased correct identification performance in both children and adults and no release of VO was found with delay. [less ▲]

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See detailNodal staging of head and neck squamous cell carcinomas : impact of a dedicated PET/CT protocol.
MINON, AL.; CABAY, Jean-Evrard ULg; DEMEZ, Pierre ULg et al

in Journal of Nuclear Medicine (The) (2012), 53(SUPPL), 1354

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See detailPilot Trial of STAR in Medical ICU
Fisk, LM; Le Compte, A; Shaw, GM et al

in Critical Care (2012), 16 (Suppl 1)

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See detailA Search For 15NH2 Lines In Comet C/2002 T7 (LINEAR)
Rousselot, Philippe; Pirali, O.; Jehin, Emmanuel ULg et al

in Bulletin of the American Astronomical Society (2012), 44

The determination of isotopic ratios in comets is of primary importance for a good understanding of their origin and the formation of solar system. The [SUP]14[/SUP]N/[SUP]15[/SUP]N ratio is an ... [more ▼]

The determination of isotopic ratios in comets is of primary importance for a good understanding of their origin and the formation of solar system. The [SUP]14[/SUP]N/[SUP]15[/SUP]N ratio is an interesting tracer, because of its variability among various solar system bodies. So far it has only been measured in bright comets through optical observations of the CN radical (Arpigny et al., 2003; Manfroid et al., 2009) and millimeter observations of HCN (Bockelée-Morvan et al., 2005, 2008). The measurements give for both species the same non-terrestrial isotopic composition ([SUP]14[/SUP]N/[SUP]15[/SUP]N≈150 in comets versus 272 in the Earth atmosphere), but HCN and CN are minor species. In order to get a determination of this ratio in another molecule we have searched for [SUP]15[/SUP]NH[SUB]2[/SUB] lines in a high-resolution and high signal-to-noise ratio spectrum of comet C/2002 T7 (LINEAR) obtained with the UVES spectrometer at the VLT ESO 8-m telescope (Hutsemékers et al., 2008). This work is based on a new laboratory experiment conducted with the AILES beamline spectrometer at synchrotron SOLEIL to determine the [SUP]15[/SUP]NH[SUB]2[/SUB] wavelengths by Fourier transform spectroscopy. We will present the first results obtained from these data, which have allowed to search for the first time [SUP]15[/SUP]NH[SUB]2[/SUB] emission lines in a comet. References: Arpigny et al., 2003, Science, 301, 1522 Bockelée-Morvan et al.,2005, in Comets II, ed. M. C. Festou, H. U. Keller, & H. A. Weaver (Tucson: Univ. Arizona Press), 391 Bockelée-Morvan et al., 2008, ApJ, 679, L49 Hutsemékers et al., 2008, A&A 490, L31 Manfroid et al., 2009, A&A 503, 613 [less ▲]

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See detailTi impact in C-doped phase-change memories compliant to Pb-free soldering reflow
Perniola, L.; Noe, Pascal ULg; Hubert, Q. et al

in Technical Digest - International Electron Devices Meeting, IEDM (2012)

In this paper, we present a thorough physical-chemical analysis of an engineered PCM stack, where the integration of C-doping and the use of a Ti top layer allow obtaining an Amorphous As-Deposited (A-AD ... [more ▼]

In this paper, we present a thorough physical-chemical analysis of an engineered PCM stack, where the integration of C-doping and the use of a Ti top layer allow obtaining an Amorphous As-Deposited (A-AD) phase stable against Back End-Of-Line (BEOL) thermal budget. This PCM stack is then integrated in devices, which are extensively tested in order to validate a novel pre-coding technique compliant to the Pb-free soldering reflow issue. Finally, an original design to optimize the distribution dispersion is presented. © 2012 IEEE. [less ▲]

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See detailModelling of agricultural diffuse pollution and mitigation measures effectiveness inWallonia (Belgium)
Sohier, Catherine ULg; Deraedt, Deborah ULg; Degré, Aurore ULg

in Geophysical Research Abstracts (2012), 14(EGU2012), 9672

Implementation of European directives in the environmental field and, specially, in the water management field, generates a request from policy-makers for news tools able to evaluate impact of management ... [more ▼]

Implementation of European directives in the environmental field and, specially, in the water management field, generates a request from policy-makers for news tools able to evaluate impact of management measures aiming at reducing pressures on ecosystems. In Wallonia (Southern Region of Belgium), the Nitrate Directive (EEC/676/91) was transposed into the “Walloon action plan for nitrogen sustainable management in agriculture” (PGDA1) in 2002. In 2007, a second plan was launched to reinforce some topics (PGDA2). Furthermore, the goal of “good quality” of surface waters and groundwater imposed by the Water Framework Directive poses new challenges in water management. In this context, a “soil and vadose” hydrological model is used in order to evaluate diffuse pollutions and efficiency of mitigation measures. This model, called EPICgrid, has been developed at catchment scale with an original modular concept on the basis of the field scale “water-soil-plant” EPIC model (Williams J.R., Jones C.A., Dyke P.T. (1984). A modelling approach to determining the relationship between erosion and soil productivity. Transactions of the ASAE. 27, 129-144). The model estimates, for each HRU identified into a 1km2 grid, water and nutrients flows into the plant-soil-vadose zone system (Sohier C., Degré A., Dautrebande S. (2009). From root zone modelling to regional forecasting of nitrate concentration in recharge flows – The case of the Walloon Region (Belgium). Journal of Hydrology, Volume 369, Issues 3-4, 15 May 2009, Pages 350-359). The model is used to make prospective simulations in order to evaluate the impact of measures currently performed to reduce the effect of diffuse pollution on water surface quality and groundwater quality, at regional scale. Response of the soil-vadose zone to agricultural practices modification is analyzed for the deadlines of the Water Framework Directive: 2015, 2021 and 2027, taking into account two climatic scenarios. Simulations results showed that actual measures are not sufficient in some areas and that new actions are necessary. The EPICgrid model was also used to evaluate effectiveness of further measures that could be implemented in order to reduce agricultural diffuse pollution. The increasing of catch crops in vulnerable zones has shown a limited impact in theWalloon context. The modifications of agricultural practices such as crop rotations or mineral fertilizing amounts have shown a more significant impact on water quality. Furthermore, the farmers’ practices are evaluated each year by a measuring campaign of the soil nitrogen residue after harvest. These data allow us to improve the representativeness of the EPICgrid model in areas in which agricultural practices largely differs from regional statistics. [less ▲]

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See detailOC-0501 IRRADIATION OF THE LEFT VENTRICLE OF THE HEART IS CORRELATED WITH POST-TREATMENT DYSPNEA IN NSCLC PATIENTS
Nalbantov, G.; De Ruysscher, Dirk; Vandecasteele, K. et al

in Radiotherapy & Oncology (2012), 103

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See detailMolecular evolution of the CYTH superfamily of proteins
Bettendorff, Lucien ULg; Delvaux, David ULg; Kohn, Grégory ULg et al

in FEBS Journal (2012), 279(Suppl. s1), 438

Molecular evolution of the CYTH superfamily of proteins L. Bettendorff, D. Delvaux, G. Kohn, P. Wins, B. Lakaye GIGA-Neurosciences, University of Liège, Belgium The CYTH superfamily of proteins was named ... [more ▼]

Molecular evolution of the CYTH superfamily of proteins L. Bettendorff, D. Delvaux, G. Kohn, P. Wins, B. Lakaye GIGA-Neurosciences, University of Liège, Belgium The CYTH superfamily of proteins was named after the two founding members, the CYaB adenylyl cyclase from Aeromonas hydrophila and the human 25-kDa THiamine triphosphatase (ThTPase). Members of this superfamily of proteins exist in all organisms including bacteria, archaea, plants and animals (except in birds) and can be traced back to the Last Universal Common Ancestor. They are characterized by a consensus sequence including several charged residues involved in divalent cation and triphosphate binding. Indeed, all members of the CYTH family that are characterized act on triphosphate derivatives and require at least one divalent cation for catalysis. The Nitrosomonas europaea (1) and E.coli CYTH proteins are specific inorganic triphosphatases. We propose that inorganic triphosphate (PPPi), the most simple triphosphate compound that can be imagined, is the primitive substrate of CYTH proteins. Other enzyme activities such as adenylate cyclase (in A. hydrophila), mRNA triphosphatase (in fungi and protozoans) and ThTPase (in metazoans) activities are secondary acquisitions. We show that ThTPase activity is not limited to mammals, but Sea anemone and Zebrafish CYTH proteins are already specific ThTPases and the acquisition of this enzyme activity is linked to the presence of a Trp (W53 in mammalian ThTPases) residue involved in the binding of the thiazole heterocycle of the thiamine molecule. The importance of W53 for the specificity of mammalian ThTPases is confirmed by site-directed mutagenesis. Furthermore, we propose a conserved catalytic mechanism between inorganic triphosphatases and ThTPases, based on a catalytic dyad comprising a Lys and a Tyr residue, explaining the alkaline pH optimum of CYTH proteins. (1) Delvaux et al. J. Biol. Chem 286 (2011) 34023-35 [less ▲]

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See detailExcited baryons in the 1/Nc expansion.
Matagne, Nicolas; Stancu, Floarea ULg

in AIP Conference Proceedings (2012), 1432

We briefly describe the 1/Nc expansion method for studying baryon masses. Two approaches of the large Nc excited baryons have been proposed so far. The first one, based on the Hartree picture, treats the ... [more ▼]

We briefly describe the 1/Nc expansion method for studying baryon masses. Two approaches of the large Nc excited baryons have been proposed so far. The first one, based on the Hartree picture, treats the baryon as a ground state core and an excited quark and the second one, suggested recently, considers the baryon globally, without decoupling the system. The masses of excited states of mixed orbital symmetry of nonstrange and strange baryons belonging to the lowest [70, −] multiplet are calculated in the 1/Nc expansion to order 1/Nc with the new method which allows to considerably reduce the number of linearly independent operators entering the mass formula. The status of the resonance Λ(1405) is discussed. [less ▲]

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