References of "Paper published in a journal"      in Complete repository Arts & humanities   Archaeology   Art & art history   Classical & oriental studies   History   Languages & linguistics   Literature   Performing arts   Philosophy & ethics   Religion & theology   Multidisciplinary, general & others Business & economic sciences   Accounting & auditing   Production, distribution & supply chain management   Finance   General management & organizational theory   Human resources management   Management information systems   Marketing   Strategy & innovation   Quantitative methods in economics & management   General economics & history of economic thought   International economics   Macroeconomics & monetary economics   Microeconomics   Economic systems & public economics   Social economics   Special economic topics (health, labor, transportation…)   Multidisciplinary, general & others Engineering, computing & technology   Aerospace & aeronautics engineering   Architecture   Chemical engineering   Civil engineering   Computer science   Electrical & electronics engineering   Energy   Geological, petroleum & mining engineering   Materials science & engineering   Mechanical engineering   Multidisciplinary, general & others Human health sciences   Alternative medicine   Anesthesia & intensive care   Cardiovascular & respiratory systems   Dentistry & oral medicine   Dermatology   Endocrinology, metabolism & nutrition   Forensic medicine   Gastroenterology & hepatology   General & internal medicine   Geriatrics   Hematology   Immunology & infectious disease   Laboratory medicine & medical technology   Neurology   Oncology   Ophthalmology   Orthopedics, rehabilitation & sports medicine   Otolaryngology   Pediatrics   Pharmacy, pharmacology & toxicology   Psychiatry   Public health, health care sciences & services   Radiology, nuclear medicine & imaging   Reproductive medicine (gynecology, andrology, obstetrics)   Rheumatology   Surgery   Urology & nephrology   Multidisciplinary, general & others Law, criminology & political science   Civil law   Criminal law & procedure   Criminology   Economic & commercial law   European & international law   Judicial law   Metalaw, Roman law, history of law & comparative law   Political science, public administration & international relations   Public law   Social law   Tax law   Multidisciplinary, general & others Life sciences   Agriculture & agronomy   Anatomy (cytology, histology, embryology...) & physiology   Animal production & animal husbandry   Aquatic sciences & oceanology   Biochemistry, biophysics & molecular biology   Biotechnology   Entomology & pest control   Environmental sciences & ecology   Food science   Genetics & genetic processes   Microbiology   Phytobiology (plant sciences, forestry, mycology...)   Veterinary medicine & animal health   Zoology   Multidisciplinary, general & others Physical, chemical, mathematical & earth Sciences   Chemistry   Earth sciences & physical geography   Mathematics   Physics   Space science, astronomy & astrophysics   Multidisciplinary, general & others Social & behavioral sciences, psychology   Animal psychology, ethology & psychobiology   Anthropology   Communication & mass media   Education & instruction   Human geography & demography   Library & information sciences   Neurosciences & behavior   Regional & inter-regional studies   Social work & social policy   Sociology & social sciences   Social, industrial & organizational psychology   Theoretical & cognitive psychology   Treatment & clinical psychology   Multidisciplinary, general & others     Showing results 201 to 300 of 4810     1 2 3 4 5 6 7 8     Intérêt des ondes de choc dans le traitement des fasciites plantairesFromm, Aurélien; Vanderthommen, Marc ; LAURENT, Jean-Marie et alin European Journal of Sports Medicine (2013, September), 1(Supplement 1), 276-277Introduction : Over the past ten years, radial shock wave therapy usage has grown tremendously. Prescribed for chronic tendinopathy, its real benefit has yet to be demonstrated. Objective : Demonstrate ... [more ▼]Introduction : Over the past ten years, radial shock wave therapy usage has grown tremendously. Prescribed for chronic tendinopathy, its real benefit has yet to be demonstrated. Objective : Demonstrate radial shock wave therapy benefits in plantar fasciitis treatment. Materials and methods : Our experimentation is a study over twelve weeks divided in 2 periods: the control phase (week 0 to week 6) and treatment phase (week 6 to week 11). It includes 3 evaluations (T1, T2 and T3) respectively at 0, 6 and 11 weeks. A standard evaluation consists of one VAS pain, an algo-functional backwash survey about the ankle (modified FFI), algometer test and dynamic analysis of the foot on strength platform. The radial shock wave therapy has been performed thanks to Swiss DolorClast machine (Electro Medical Systems) Results : Statistical analysis show radial shock wave therapy induces a highly significant improvement (p<0,0001) on EVA results (50%), ankle algo-functional backwash (62.6%) and pain sensitivity threshold (49.6%). Therefore, a significant correlation can be observed during T2- T3 period between pain sensitivity threshold improvement and patients age (p=0,012). The statistical results show a time increase trend for foot-ground contact over time. It is not significant though. Conclusion : The use of radial shock wave therapy in plantar fasciitis treatment helps decrease pain and improve step. However, additional studies performed on bigger samples have to be completed to confirm these results. [less ▲]Detailed reference viewed: 197 (14 ULg) Immunohistochemical transforming growth factor Beta1 and its activation, storage and signalling pathways in canine idiopathic pulmonary fibrosisKrafft, Emilie ; Laurila, HP; Rajamäki, MM et alin Proceedings of the 23th ECVIM Meeting (2013, September)Detailed reference viewed: 11 (2 ULg) Long term follow up in dogs with eosinophilic bronchopneumopathy treated with inhaled glucocorticosteroid therapy preliminaryCanonne-Guibert, Morgane ; Bolen, Géraldine ; Billen, Frédéric et alin Proceedings of the 23th ECVIM Meeting (2013, September)Detailed reference viewed: 30 (6 ULg) GP’s engagement in detecting and managing abuse of alcohol, illegal drugs, hypnotics and tranquilizers in the Belgian adult populationKetterer, Frédéric ; Lambrechts, Marie-Claire; Symons, Linda et alin European Journal of General Practice (2013, September), 19(3), 162-184Background General practitioners (GPs) Occupational physicians (OPs) can play an important role in detecting and managing substance abuse in the working population. The UP TO DATE project aims at ... [more ▼]Background General practitioners (GPs) Occupational physicians (OPs) can play an important role in detecting and managing substance abuse in the working population. The UP TO DATE project aims at identifying the difficulties these professionals encounter in this area, and to explore ways of collaboration in providing appropriate care. Research question What are the experiences, attitudes and decision making of GPs and OPs regarding to alcohol, illegal drugs, hypnotics and tranquilizers abuse from a physician’s perspective? Method In this qualitative study, with a phenomenological perspective, 20 GPs and 16 OPs, experienced with substance abuse in daily practice, got a face to face in-depth interview. (October until December 2012). Along with the data collection process the analysis started with a constant comparison between the data and the chosen integrated model of change (De Vries), using coding techniques of grounded theory. Results GPs meet important barriers to detect misuse of all substances. To address this issue and the patient’s motivation for change, the doctor-patient relationship is crucial. The risk to disturb the relationship, and loose the patient’s trust, is a major concern. An attitude of patient-centeredness, collaboration and empowerment of the patient is needed to make any progress, to get results and to overcome as a GP the burden of those demanding encounters. Self-care is an important concern. Collaboration with specialised health providers confronted GPs with various problems: not enough services, long waiting lists, unclear methods and criteria. GPs lack an insight in the OP’s role, and experience problems to contact them. Whether the OP is a trustful health advocate for their patient is a major concern. Some OPs are perceived too linked to the employer, raising legitimate concerns about professional confidentiality. Conclusion These are preliminary results. At the conference, a comprehensive overview on the qualitative analysis will be given. [less ▲]Detailed reference viewed: 52 (19 ULg) Effect of the lengthening of the protocol on the reliability of knee muscle fatigue indicatorsBosquet, L.; Maquet, Didier ; Forthomme, Bénédicte et alin European Journal of Sports Medicine (2013, September), 1(1), 150Detailed reference viewed: 30 (8 ULg) Primate Behaviour and Parasite Transmission in the Ecuadorian AmazonMartin, Sarah ; Carrillo Bilbao, Gabriel Alberto; Bernstein, Sophie et alin Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013, August 10), 84(3-5), 135-346Detailed reference viewed: 15 (5 ULg) A Methodology for The Appropriation And The Isolation of Nodal Diameter Modes in Cyclic Symmetric StructuresNyssen, Florence ; Viguié, Régis; Simon, Daniel et alin Proceedings of ASME 2013 International Design Engineering Technical Conference & Computers and Information in Engineering Conference IDETC/CIE 2013 (2013, August)This paper presents a methodology enabling the appropriation and the isolation of a chosen nodal diameter modes in cyclic symmetric structures. In particular, a study about the minimum number and the ... [more ▼]This paper presents a methodology enabling the appropriation and the isolation of a chosen nodal diameter modes in cyclic symmetric structures. In particular, a study about the minimum number and the optimized location of the excitation sources required in laboratory conditions to isolate a specific nodal diameter mode is conducted. This work includes a theoretical approach based on energy concepts. It is followed by the numerical and experimental validations on a stator stage of an axial compressor. The concept of modal appropriation is investigated here as the first step of the calibration procedure before engine tests. [less ▲]Detailed reference viewed: 46 (16 ULg) Increased Inflammasome and Caspase-1 Activation in Visceral adipose tissue from Metabolically Unhealthy Obese compared to Metabolically Healthy Obese subjectsEsser, Nathalie ; L'Homme, Laurent ; DE ROOVER, Arnaud et alin Diabetes (2013, July), (62/suppl.1), 555Detailed reference viewed: 37 (12 ULg) Direct use of MACE EBV in the Walloon single-step Bayesian genomic evaluation systemVandenplas, Jérémie ; Colinet, Frédéric ; Faux, Pierre et alin Journal of Dairy Science (2013, July), 96(E-Supplement), Detailed reference viewed: 13 (7 ULg) Platelet-rich plasma (PRP) to treat chronic upper patellar tendinopathiesKaux, Jean-François ; Croisier, Jean-Louis ; Bruyère, Olivier et alin British Journal of Sports Medicine (2013, July), 47(10 (e3)), 15Background: Upper patellar tendinopathies remain often chronic and rebel to a thorough conservative treatment. Moreover, the option of a surgical treatment could be disappointing. New treatments are being ... [more ▼]Background: Upper patellar tendinopathies remain often chronic and rebel to a thorough conservative treatment. Moreover, the option of a surgical treatment could be disappointing. New treatments are being developed. Injection of PRP is one of these. Platelets contain lot of growth factors which would have the potentiality to enhance the healing process of tendons. Even if in vitro and animal experiments have demonstrated this stimulation of tendon healing process1, clinical series are subject to controversy2. Methods: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments ［VAS, clinical examination with an algometer, algofunctional scores (IKDC and VISA-P), functional assessments (isokinetic and Optojump) and imagery (ultrasounds and MRI)］were made before infiltration of PRP, and 6 weeks and 3 months after. The PRP was obtained by an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a 6-week standardised sub-maximal eccentric reeducation. Results: We observed a very significant improvement of the algofunctional status as soon as 6 weeks after the infiltration of PRP, and continued to a lesser extent up to 3 months. During functional evaluation, pain decreased as well, but without significant improvement of performances. No significant improvements in the imagery were observed. Interestingly, patients who had a VAS equal or below 1 after 3 months post-infiltration were younger (24.7 vs 32.2 y.o.). Moreover, these younger patients had a significant increase of the IKDC score (p=0.003), a significant improvement of pain during isokinetic evaluations (p<0.05), and during Optojump assessments (p=0.01). Seventy-five percent of subjects were able to return to sport, even if only half of these patients recovered the same level than before the tendinopathy. Discussion / Conclusions: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol is efficient to improve symptoms of chronic upper patellar tendinopathies, non-responsive to classical conservative treatments. However, up to now, there is no consensus on the method to prepare the PRP. Indeed, each technique could provide a very different PRP (variations in the platelet concentrations and of the amount of red and white cells). [less ▲]Detailed reference viewed: 89 (24 ULg) Mosquito net for abdominal wall repair: a comparison of their chemical, physical, morphological and mechanical properties to commercial meshesSevrin, Chantal ; Nsadi Fwene, Berthier; Arung, Willy et alin Biomedica proceedings (2013, June 23)Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor ... [more ▼]Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor developing countries a traditional sutured repair is still commonplace although with significantly inferior results. Sterilized mosquito nets have been recently roposed by several authors to replace medical meshes in low-income countries . However in the perspective of their clinical use a better understanding of this mosquito net is obviously requested both in terms of material properties and of in vivo biocompatibility. [less ▲]Detailed reference viewed: 32 (7 ULg) Can we use electrical resistivity tomography to measure root zone competition in fields with multiple crops?Garré, Sarah ; Coteur, Ine; Wongleecharoen, Chalemchart et alin Procedia Environmental Sciences (2013, June 20)Contour hedgerow intercropping systems have been proposed to reduce run-off and control soil erosion on steep agricultural land. However, competition for water and nutrients between crops and associated ... [more ▼]Contour hedgerow intercropping systems have been proposed to reduce run-off and control soil erosion on steep agricultural land. However, competition for water and nutrients between crops and associated hedgerows may reduce the overall performance of these systems. ERT measurements conducted in Thailand showed that the soils of our experimental plots were very heterogeneous both along the slope as with depth. This observation highlighted some constraints of the ERT method for soil moisture monitoring in the field. Nevertheless, the data indeed revealed contrasting water depletion patterns under monocropping and intercropping systems, which could also be related to plant parameters. [less ▲]Detailed reference viewed: 48 (3 ULg) Excitation of nodal diameter mode-shapes of a stator ring of a turbojet engine using a limited number of excitation sourcesNyssen, Florence ; Golinval, Jean-Claude ; Viguié, Régis et alin Proceedings of ASME Turbo Expo 2013 (2013, June 05)When considering engine tests, the sensors used to monitor the rotor parts mainly consist in strain gauges whose information is very sensitive to the gauge positioning, especially in high stress gradient ... [more ▼]When considering engine tests, the sensors used to monitor the rotor parts mainly consist in strain gauges whose information is very sensitive to the gauge positioning, especially in high stress gradient regions. Therefore, to reduce the risk of misinterpreting the response given by the gauges, their calibration is required. Because strain gauges are positioned so that specific modes will be monitored, their calibration must be carried out for these modes. For cyclic symmetric structures, their high spectral density imposes the use of dedicated calibration techniques. In the present paper, the concept of modal appropriation is investigated as the first step of the calibration procedure. This paper aims at presenting the development of an experimental approach enabling the isolation/appropriation of specific nodal diameter modes of a stator stage of an axial compressor using a limited number of excitation sources. A special emphasis has been brought to the minimum number and the optimized location of the excitation sources (shakers) required in laboratory conditions to appropriate specific nodal diameter modes and thereby retrieve the related modal features needed for the gauges calibration and mechanical design. [less ▲]Detailed reference viewed: 60 (20 ULg) Ambulatory Monitoring of Teachers' Voice UseRemacle, Angélique ; Morsomme, Dominique ; FINCK, Camille in AQL Proceedings (2013, June)Method: Thirty-two French-speaking female teachers were monitored during one workweek (5 days) using the Ambulatory Phonation Monitor, Model 3200 (KayPENTAX). Phonation time, fundamental frequency (F0 ... [more ▼]Method: Thirty-two French-speaking female teachers were monitored during one workweek (5 days) using the Ambulatory Phonation Monitor, Model 3200 (KayPENTAX). Phonation time, fundamental frequency (F0), sound pressure level (SPL), distance dose and cycle dose were analyzed in both professional and non-professional environments. Results: Teachers’ phonation time was statistically higher at school (20.3% ± 4.2) than not at school (10.4% ± 3.8). Concerning the fundamental frequency, both F0 average and F0 mode were statistically higher in the professional environment (F0 average = 258.7 Hz ± 20.5; F0 mode = 229.7 Hz ± 18.3) than in the non-professional environment (F0 average = 239.6 Hz ± 20.1; F0 mode = 213.3 Hz ± 20.6). The teachers spoke statistically louder at school (80.6 dB SPL ± 4.9) than not at school (74.5 dB SPL ± 5.2 dB SPL). The cycle dose and the distance dose were also statistically higher at school than in the non-professional environment. The data showed that the vocal folds vibrate 1,195,834 (± 255,696) times a day at work, plus 425,102 (±194,338) times after work. The distance traveled by the vocal folds for these teachers was 4,247 (± 1,476) meters at work, plus 1,173 (± 527) meters after work. Conclusion: Professional and non-professional voice use comparisons showed significant differences for all parameters (p<.001), indicating that vocal load was higher in the professional environment compared to the non-professional environment. [less ▲]Detailed reference viewed: 48 (16 ULg) Exuberant inflammatory reaction after an infiltration of platelet-rich plasma (PRP)Kaux, Jean-François ; Croisier, Jean-Louis ; LEONARD, Philippe et alin British Journal of Sports Medicine (2013, June), 47(10 (e3)), 92Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively ... [more ▼]Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively new treatment for tendinopathies. To date, no side effects have been reported after infiltration of PRP to treat tendinopathy. Case report: A 35-year-old patient had a right upper patellar tendinopathy which was resistant to all conservative treatments for more than 6 months. The patient was a type 1 diabetic (well controlled). He had an intratendinous infiltration of 6 mL of PRP (8.105 platelets/mm3, almost no red or white blood cells) after disinfection but without local anaesthetic. Immediately following the infiltration, local cryotherapy was performed for 15 minutes. NSAIDs were avoided, but class-1 or -2 pain-killers were authorised if necessary. A standardised sub-maximal eccentric rehabilitation should have been started 1 week after. However, the patient experienced local swelling with erythema, increased heating and pain which appeared just underneath the patella, but without biological inflammatory syndrome. A great Doppler signal in a thicker patellar tendon was observed by US, but there was no sign of local infectious disease demonstrated by either CT or MRI. However, the local inflammation did not decrease after a progressive 3-week treatment of local cryotherapy, local and oral NSAIDs and colchicine 1 mg. Thus, an insidious infection was suspected, even though there was neither evidence of biological inflammatory syndrome nor sign of infectious lesion on imagery examination. An antibiotic therapy (rifampicine 600 mg + minocycline 100 mg) was initiated for 3 months. Finally, a 3-phase bone scintigraphy suggested the presence of a complex regional pain syndrome type 1 treated by a classical physical therapy and concomitant class-2 pain killers. The evolution was favourable after 6 months of symptomatic treatment, and the pain decreased to a level similar to that before the infiltration of PRP. Discussion/Conclusions: This case report draws attention to potential side effects that are linked to this new therapy by infiltration of platelet rich plasma in case of tendinopathy, in particular when used in patients with type 1 diabetes. Thus, the balance between benefits and risks must be carefully evaluated before using this treatment in patients with type 1 diabetes. Reference: Platelet-rich plasma application in the management of chronic tendinopathies. Acta Orthop Belg 2013; 79: 10-15. [less ▲]Detailed reference viewed: 68 (15 ULg) Peuple(s) et subjectivations politiques chez Claude Lefort: une lecture du "MachiavelJanvier, Antoine ; Mancuso, Eva in Tumultes (2013, June), 40Detailed reference viewed: 36 (22 ULg) INNOVATIVE BOLTED BEAM-TO-COLUMN JOINTS IN MOMENT RESISTANT BUILDING FRAME: FROM EXPERIMENTAL TESTS TO DESIGN GUIDELINESHoang, Van Long ; Demonceau, Jean-François ; Jaspart, Jean-Pierre in Application of High strength steels in Seismic Resistant Structures (2013, June)Detailed reference viewed: 5 (1 ULg) Assessing meteorological conditions effects on MIR predicted methane emissions of Holstein cows under a temperate environmentVanrobays, Marie-Laure ; Gengler, Nicolas ; Soyeurt, Hélène et alin Advances in Animal Biosciences (2013, June), 4(2), 488Detailed reference viewed: 21 (4 ULg) Drug related problems and interventions of pharmacists on prescribed medicines in BelgiumDe Wulf, Isabelle; Boussery, Koen; De Vriese, Carine et alin International Journal of Clinical Pharmacy [=IJCP] (2013, June), 35(3), 497Aim of project/study (1) To study the frequency and nature of drug related problems (DRP) detected by community pharmacists and internship students when dispensing prescribed medicines. (2) To investigate ... [more ▼]Aim of project/study (1) To study the frequency and nature of drug related problems (DRP) detected by community pharmacists and internship students when dispensing prescribed medicines. (2) To investigate the nature and frequency of interventions by pharmacists. (3) To study whether there is a difference between DRP detection at the moment of dispensing versus in a quiet setting. [less ▲]Detailed reference viewed: 83 (12 ULg) High-speed imaging use to predict spray retention on barley leavesBoukhalfa, Hassina dite Hafida ; Massinon, Mathieu ; Lebeau, Frédéric in Communications in Agricultural and Applied Biological Sciences (2013, May 21), Vol 78(2)(1-386 (2013)), 31-36Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera ... [more ▼]Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera coupled with a retro-LED lighting. Size and velocity of the drop were extracted by image analysis. Drop impact types were determined by the operator. Drops were produced with a flat-fan nozzle mounted on a movable ramp. Two surfactants (Break-Thru® S240 and Li700 ®) were sprayed on BBCH 12 barley leaves to highlight the effect of the reduction of surface tension. Relative volume proportions were computed within of an energy scale divided into 11 classes based on the Weber number. results are compared to the results of the chemical analysis by spectrofluerometry. [less ▲]Detailed reference viewed: 84 (32 ULg) Silky bent grass resistance to herbicides: one year of monitoring in BelgiumHenriet, François; Bodson, Bernard ; Meza Morales, Walter in Communications in Agricultural and Applied Biological Sciences (2013, May 21), 78(3), 665-670Silky bent grass (Apera spica-venti (L.) P. Beauv.) is a common weed of cereal crops widely spread in Northern and Easthern Europe (Germany, Czech Republic, ...), Northern Asia, Sibera and Canada. Up to ... [more ▼]Silky bent grass (Apera spica-venti (L.) P. Beauv.) is a common weed of cereal crops widely spread in Northern and Easthern Europe (Germany, Czech Republic, ...), Northern Asia, Sibera and Canada. Up to now, no resistant case has been detected in Belgium but some chemical weeding failures have been observed in Wallonia fields. During summer 2011, 37 seed samples of Apera spica-venti were collected in Wallonia and submitted to resistance tests in controlled conditions. Three modes of action were tested: acetyl coenzyme-A carboxylase inhibitors (pinoxaden and cycloxydim), acetolactate synthase inhibitores (mesosulfuron+iodosulfuron, pytroxsulam and sulfometuron) and photosynthesis inhibitors (isoproturon). One susceptible standard population was included in the test in order to validate it and to permit wild populations classification according to "R" rating system developed by Moss et al (2007). Most of populations were susceptible but some populations showed resistance to at least one of the three tested modes of action. [less ▲]Detailed reference viewed: 16 (1 ULg) Comparison of the total error obtained with six immuno-assays for 25-hydroxy vitamin D with the minimal, desirable and optimal total allowable errors calculated according to the biological variationCAVALIER, Etienne ; LE GOFF, Caroline ; CARLISI, Ignazia et alin Biochimica Clinica (2013, May), 37(SS), 259Detailed reference viewed: 28 (2 ULg) Comparison of the Heart-type fatty acid-binding protein (H-FABP) with the high sensitive cardiac troponin T in healthy runnersLE GOFF, Caroline ; Kaux, Jean-François ; BREVERS, Eric et alin Biochimica Clinica (2013, May), 37(SS), 544Background: Heart-type fatty acid-binding protein (H-FABP) is a low molecular weight protein involved in the intracellular uptake and buffering of long chain fatty in the myocardium. It is an early marker ... [more ▼]Background: Heart-type fatty acid-binding protein (H-FABP) is a low molecular weight protein involved in the intracellular uptake and buffering of long chain fatty in the myocardium. It is an early marker for acute coronary syndrome. Troponin T (TnT) is a component of the contractile apparatus of the striated musculature. Cardiac TnT is a cardio-specific, highly sensitive marker for myocardial damage. The aim of our study was to compare the results obtained with the H-FABP and the highly sensitive cardiac troponins (hsTnT) and to test their cardiospecificity in healthy runners. Methods: Twenty three runners (marathon) were enrolled. We drowned samples at three times: just before (T0), just after (T1), and three hours after the end of the race (T3). H-FABP was determined with a Randox immunoturbidimetric assay and hs-TnT with a Roche electrochemiluminescence immunoassay, both on Cobas 6000. A linear regression was calculated to observe if there is any correlation between the two biomarkers. Values above the 95th percentile for H-FABP (2.5ng/mL) and the 99th percentile for hsTnT (14ng/L) were considered as positive. Results: At T0, none of the subjects were positive for hsTnT but 35% were positive for H-FABP; at T1, 83% for hsTnT and 100% for H-FABP; at T3, 83% for hsTnT and 96% for H-FABP. At T0, the regression equation was H-FABP T0 = 3.9454 – 0.1001 x hsTnT T0; at T1: H-FABP T1 = 51.838 – 1.7026 x hsTnT T1; at T3: H-FABP T3 = 47.977 – 1.6193 x hsTnT T3. No correlation was observed between the two biomarkers at the different time. Conclusions: We observed a significant increase of H-FABP and hsTnT in runners. These markers are independent to each other. These values could biologically correspond to a heart ischemia. However, we suggested that exercise-induced cardiac hsTnT and H-FABP release is not a marker of exercise-induced pathology but likely a physiologic response to effort or an exercise-induced cardiac remodelling. [less ▲]Detailed reference viewed: 24 (0 ULg) Creatinine-or cystatin C-based equations to estimate glomerular filtration: impact on the epidemiology of chronic kidney diseaseDELANAYE, Pierre ; Bruyère, Olivier ; Moranne, O et alin Biochimica Clinica (2013, May), 37(SS), 217Detailed reference viewed: 10 (4 ULg) Heavy/light chain (HLC) and free light chain (FLC) analysis allow sensitive monitoring of multiple myeloma patients and AID detection of clonal changesDierge, Laurine; LUTTERI, Laurence ; Chauvet, D et alin Biochimica Clinica (2013, May), 37(SS), 618Detailed reference viewed: 40 (4 ULg) Evaluation of the performance of the new automated IDS-ISYS 1,25-dihydroxy vitamin D assayGADISSEUR, Romy ; LUKAS, Pierre ; CARLISI, Ignazia et alin Biochimica Clinica (2013, May), 37(SS), 498Detailed reference viewed: 25 (7 ULg) Determination of urinary metanephrine, normetanephrine and methoxymetanephrine by liquid chromatography-electrospray tandem mass spectrometry.LE GOFF, Caroline ; PEETERS, Stéphanie ; NETCHACOVITCH, Matthieu et alin Biochimica Clinica (2013, May), 37(SS), 316Background: The aim of this work was to develop and validate a method for the determination of metanephrine (M), normetanephrine (NM) and methoxymetanephrine (METHO) in urine by liquid chromatography ... [more ▼]Background: The aim of this work was to develop and validate a method for the determination of metanephrine (M), normetanephrine (NM) and methoxymetanephrine (METHO) in urine by liquid chromatography-tandem mass spectrometry (LCMS-MS) on the Triple Quad TQ 5500 from AB SCIEX. In fact, the determination of M and NM concentrations is used in clinical diagnosis of pheochromocytoma, a rare but potentially fatal tumor arising primarily from the chromaffin cells of the adrenal medulla. Methods: The samples were made of 24 hours acidified urines after centrifugation. Sample preparation was performed by hydrolysing and purifying by extraction column. After that, labeled M, NM and METHO were added as internal standard. Samples were analysed by liquid chromatography-electrospray tandem mass spectrometry. We determined the repeatability, reproducibility, accuracy profile and recovery on pooling urines samples from 9 volunteers analysed in triple run. Results: The results of the precision evaluation are shown in table. The repeatability did not exceed 8.4 % for M, 6.8% for NM and 10.8% for METHO. The concentration range was 71-781 µg/24h, 71-853 µg/24h and 20-854µg/24h for the M, NM and METHO respectively. The total precision did not exceed 12.5%, 11.8% and 8.8% for M, NM and METHO. The limit of quantification (LOQ) were 33.77µg/24h, 14.49µg/24H and 19.81 µg/24H for M, NM and METHO respectively. The accuracy varied from 99.69 to 100.2% for a range of 71 to 781 µg/24h, from 93.32 to 100.2% for a range of 71-853 µg/24h and from 99.85 to 100.6% for the range 20-854µg/24h for M, NM and METHO respectively. The recovery were 99.96% (95% CI for the mean: 96.5-103.4), 99.75% (96.5-102.9) and 100.08 (95.97-104.2) for the M, NM and METHO respectively. Conclusions: We have successfully developed and validated an LCMS-MS method to determine urinary M, NM and METHO on the TQ 5500 from AB SCIEX. It represents a convincing alternative to the HPLC method for a faster and reliable measurement of urinary M, NM and METHO. [less ▲]Detailed reference viewed: 47 (9 ULg) Association of the circulating inactive form of matrix GLA protein (DP-UCMGP) levels and abdominal aortic calcification score (AACS) in haemodialysis patientsCAVALIER, Etienne ; GADISSEUR, Romy ; LUKAS, Pierre et alin Biochimica Clinica (2013, May), 37(SS), 215Detailed reference viewed: 18 (7 ULg) Race Cycling: biological evolutionLE GOFF, Caroline ; Kaux, Jean-François ; MELON, Pierre et alin Biochimica Clinica (2013, May), 37(SS), 544Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 179.6 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cell (RBC), haemoglobin (HgB), creatinin (Cr), highly sensitive troponin T (hsTnT), myoglobin (MYO) and NT-proBNP. All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results: RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002). Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001). These changes might be related to renal flow depletion during exercice. MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014). The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001) .The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP. These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusion: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]Detailed reference viewed: 22 (0 ULg) Evaluation of the third-generation to second-generation PTH ratio on the Liaison XL (DIASORIN) as a marker for parathyroïd carcinomaSchleck, Marie-Louise; LAURENT, Terry ; GADISSEUR, Romy et alin Biochimica Clinica (2013, May), 37(SS), 161Detailed reference viewed: 28 (13 ULg) Biophysical characterization of the interaction of novel aromatic glycolipid surfactants with membrane models.Sainvitu, Pauline ; Nasir, Mehmet Nail ; Nott, Katherine et alin World Academy of Science, Engineering and Technology (2013, May), 77Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic ... [more ▼]Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as a function of their aromatic group. Their interaction with membranes was studied with monolayer models and was predicted by a computational approach. The relationships between these data and their cytotoxicity and antioxidant properties evaluated on cell cultures are discussed. [less ▲]Detailed reference viewed: 15 (9 ULg) Creatinine- or cystatin C-based equations to estimate glomerular filtration rate in the general population: impact on the epidemiology of chronic kidney diseaseDELANAYE, Pierre ; Cavalier, Etienne ; Moranne, O et alin Nephrology Dialysis Transplantation (2013, May), 28(S1), Detailed reference viewed: 19 (15 ULg) Biophysical characterization of the interaction of novel aromatic glycolipid surfactants with membrane models.Sainvitu, Pauline ; Nasir, Mehmet Nail ; Nott, Katherine et alin World Academy of Science, Engineering and Technology (2013, May), 77Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic ... [more ▼]Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as a function of their aromatic group. Their interaction with membranes was studied with monolayer models and was predicted by a computational approach. The relationships between these data and their cytotoxicity and antioxidant properties evaluated on cell cultures are discussed. [less ▲]Detailed reference viewed: 16 (3 ULg) Holotranscobalamin as an earlier indicator of vitamin B12 deficiency in thyrogastric syndromeVRANKEN, Laura ; CAVALIER, Etienne ; VALDES SOCIN, Hernan Gonzalo in Biochimica Clinica (2013, May), 37(SS), 324Detailed reference viewed: 21 (9 ULg) Is ultra-short cold ischemia the key to ischemic cholangiopathy avoidance in DCD-LT?DETRY, Olivier ; DE ROOVER, Arnaud ; Cheham, S et alin Acta Chirurgica Belgica (2013, May), Supplement 113(3), 6729Detailed reference viewed: 40 (12 ULg) Difference between total and intact assays for N-Terminal propeptide of type I procollagen (P1NP) determination in renal impaired patientsCAVALIER, Etienne ; LUKAS, Pierre ; FERRANTE, Nunzio et alin Biochimica Clinica (2013, May), 37(SS), 498Detailed reference viewed: 11 (5 ULg) Comparison between Perkin-Elmer and Chromsytem Vitamin D kit on TQ 5500 from AB SCIEXLE GOFF, Caroline ; PEETERS, Stéphanie ; CRINE, Yannick et alin Biochimica Clinica (2013, May), 37(SS), 471Background: Twenty-five hydroxy-vitamin D (25(OH) D) determination is now routinely prescribed in the Laboratory. Recently, different new methods have been available for this determination. Among them ... [more ▼]Background: Twenty-five hydroxy-vitamin D (25(OH) D) determination is now routinely prescribed in the Laboratory. Recently, different new methods have been available for this determination. Among them, LCMS/MS methods have emerged in some laboratories. However these methods are generally “home-brewed” and an important variability between them can be seen on different external quality controls, mainly due to a lack of standardization. Recently, Perkin-Elmer (PE) (Turku, Finland) and Chromsystem (CS) (Grafelfing, Germany) launched a standardised method for 25(OH )D determination on LCMS/MS. The aim of our study was to compare these methods on the AB SCIEX TQ5500 (Framingham, Massachusetts, USA) LCMS/MS to measure 25(OH) D3. Methods All the samples were treated according to our preanalyitical procedure: after sampling, they were spun at +4°c at 3500G, aliquoted and kept frozen at -20°c until determination. A method comparison was assessed with CS and PE for the measurement of the 25(OH)D3. We selected 110 remnant samples with 25(OH)D3 levels ranging from 1.6 to 136.7 ng/ml with the PE method to cover the range of usually values Slope and intercept were calculated using Passing and Bablock linear regression and we compared the methods with the Bland and Altman plots. Results For CS, the method is linear up to 250 µg/L, the LOQ is 3.6 µg/L, the intra-assay CV is < 5% and the inter-assay is < 7%. For PE, the method is linear up to 314 µg/L, the LOQ is 3.4 µg/L, the intra-assay CV is < 7.8% and the inter-assay is < 8.5%. On the whole range of measure (n=110), the regression equation is PE = 0.8521+0.9226 (CS) (95%CI of the intercept: (-0.0048;1.37) and 95% CI of the slope (0.89;0.95). The Bland and Altman plot does not show any bias between the two methods (mean difference CS-PE= -2.5 ng/ml) and the standard deviation of the mean is 3,98 ng/ml Conclusion: The performances of these methods are comparable on our new TQ 5500 from AB SCIEX. For now, there is no consensus on a “reference” method for vitamin D quantification. We notice only that the values obtained by CS are systematically a little bit lower than PE’s values, especially for results below 20 ng/ml. However, we have no clear explanation for such behaviour. [less ▲]Detailed reference viewed: 90 (7 ULg) Therapeutic Strategy and Patient Outcome during the First 2 Years of Pediatric Crohn’s DiseaseVeereman, G; Mahachie John, Jestinah ; De Greef, E et alin Journal of Pediatric Gastroenterology and Nutrition (2013, May)Detailed reference viewed: 31 (7 ULg) Impact of strenuous exercise on the release of cardiac biomarkersLE GOFF, Caroline ; MELON, Pierre ; Kaux, Jean-François et alin Biochimica Clinica (2013, May), 37(SS), 545Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured ... [more ▼]Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured by new commercially available high-sensitive methods in subjects un-dergoing the Maasmarathon. Our aim was to compare cTnT and NT-proBNP levels before and after the stress tests, in sportive subjects. Methods: Twenty eight subjects (26 ♂, 42.5±11yo) underwent a race of 42.195 kilometers be-tween Visé (Belgium) and Maastricht (The Netherlands). We drowned blood sample before (T0), just after (T1) and three hours after the race (T3). In all patients, cTnT concentrations were measured by high sensitive methods (hsTnT, Roche Diagnostics) on heparin plasma. The NT-proBNP was also determined with the kit Roche on heparin plasma. The protocol was approved by the ethics committee of the University of Liège (Belgium). All subjects gave their informed consent. All statistical analyses were performed using Medcalc version 8.1 for Windows. p-value <0.01 was regarded as statistically significant. Results: There was a significant difference between hsTnT concentrations at T0 and T1 (p<0.0001), and between T0 and T3 (p<0.001) for NT-proBNP, but not between T1 and T3. This observation appeared only after a strenuous exercise but today this type of exercise is not reproduce easier in a laboratory of sport. Moreover, at this moment, nobody knows if these observations would have cardiac consequences at long terms. Conclusions: Measurement of cardiac troponins by high sensitive methods allows detecting significant release of biomarkers from the heart during exercise. The value of NT-proBNP are also significant but less than TnThs. We think that the TnThs could be an interesting tool in the future to help sport medicine to detect risk of developing a cardiac problem in the future or a sudden death. [less ▲]Detailed reference viewed: 37 (8 ULg) MDRD VERSUS CKD-EPI EQUATIONS TO ESTIMATE GLOMERULAR FILTRATION RATE IN OBESE PATIENTSBOUQUEGNEAU, Antoine ; CAVALIER, Etienne ; Krzesinski, Jean-Marie et alin Nephrology Dialysis Transplantation (2013, April 18), 28(supplément 1), 117-139Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially ... [more ▼]Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially important to follow these patients. We have tested the performances of two creatinine-based equations, namely the MDRD and CKD-EPI equations, in an obese population. Conclusions :Both in the global and subgroup analyses, the CKD-EPI equation did not outperform the MDRD study equation. The performances of both equations were worse in CKD patients. These two conclusions were still valid if indexed GFR was considered. [less ▲]Detailed reference viewed: 20 (1 ULg) Immune Reconstitution After Alternative Hematopoietic Stem Cell Transplantation: Comparison of Unrelated Cord Blood and Mismatched Unrelated Donor Hematopoietic Stem Cell TransplantationServais, Sophie ; Lenglinne, Etienne; Porcher, Raphael et alin Bone Marrow Transplantation (2013, April 08), 48(S2), 132Detailed reference viewed: 17 (3 ULg) Effect of a single oral loading dose of 100,000 IU of cholecalciferol on serum 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D concentrations in healthy subjectsLAURENT, Terry ; LUKAS, Pierre ; ROUSSELLE, Olivier et alin Osteoporosis International (2013, April), 24(S1), 279Detailed reference viewed: 46 (2 ULg) Cost-effectiveness of vitamin D and calcium supplementation in the treatment of postmenopausal womenHiligsmann, Mickaël ; Ben Sedrine, Wafa ; Rabenda, Véronique et alin Osteoporosis International (2013, April), 24(Suppl.1), 198Detailed reference viewed: 30 (5 ULg) Superior Renal Function Sustained for 24 Months through Early Everolimus-Facilitated Reduction of Tacrolimus Versus Standard Tacrolimus in De Novo Liver Transplant Recipients: Results of a Randomized Trial.De Simone, Pierre; DETRY, Olivier ; Kintmalm, G et alin American Journal of Transplantation (2013, April), 13(S5), 169450mTOR inhibitors have the potential to reduce calcineurin inhibitor nephrotoxicity by minimizing or eliminating the need for their use. The 12 month (M) results of H2304 (NCT00622869) study demonstrated ... [more ▼]mTOR inhibitors have the potential to reduce calcineurin inhibitor nephrotoxicity by minimizing or eliminating the need for their use. The 12 month (M) results of H2304 (NCT00622869) study demonstrated superior renal function with everolimus (EVR) plus reduced tacrolimus (rTAC) vs. standard TAC (TAC-C) in de novo liver transplant recipients (LTxR). Presented here are 24M renal function results. For this 24M, multicenter, open-label study 719 de novo LTxR were randomized (1:1:1) after a 30-day (±5 days) run-in period with TAC (±mycophenolate mofetil), to receive either EVR (C0 3-8 ng/mL) with rTAC (C0 3-5 ng/mL; EVR+rTAC, N=245) or EVR (C0 6-10 ng/mL) with TAC withdrawal (TAC-WD; N=231) at M4 or TAC-C (C0 6-10 ng/mL; TAC-C, N=243); all patients received corticosteroids. Enrollment in TAC-WD arm was stopped early due to higher rejection rates. Main endpoints at M24 included composite ef􏰀cacy failure rate of treated biopsy proven acute rejection, graft loss or death, and evolution of renal function from randomization (RND) to M24 measured as eGFR by MDRD4. At M24, composite ef􏰀cacy failure rate in EVR+rTAC arm was comparable to TAC-C (10.3% vs. 12.5%, p=0.452). Evolution of renal function from RND to M24 was superior for EVR+rTAC vs. TAC-C with an adjusted mean difference in eGFR change of 6.66 mL/min/1.73m2 (p=0.0018; ITT population). Signi􏰀cantly higher eGFR with EVR+rTAC was achieved at M2 post-LTx and was maintained until M24. On-treatment data showed a decrease in mean eGFR from RND to M24 of 6.6 mL/min/1.73m2 with EVR+rTAC vs. 13 mL/min/1.73m2 with TAC-C and 2.5 mL/ min/1.73m2 gain with TAC-WD. Urinary protein:creatinine ratio (mg/g) at M24 was higher with EVR+rTAC vs. TAC-C (Mean±SD: 194±280 vs. 159±284, p=0.006). Early introduction of EVR at 1M post-LTx with rTAC showed superior renal function sustained for 24M compared to TAC-C, without compromising ef􏰀cacy in de novo LTxR. [less ▲]Detailed reference viewed: 40 (2 ULg) Equivalence of a single dose (1200mg) compared to a 3-time a day dose (400mg) of chondroitin 4&6 sulfate in patients with knee osteoarthritis: Results of a randomized double blind placebo controlled studyZegels, Brigitte ; LEONORI, Lorenzo ; Crozes, P et alin Osteoporosis International (2013, April), 24(Suppl.1), 383Detailed reference viewed: 20 (10 ULg) Effect of a single oral loading dose of 100,000 IU of cholecalciferol on bone biomarkers in healthy dults subjectsLAURENT, Terry ; LUKAS, Pierre ; ROUSSELLE, Olivier et alin Osteoporosis International (2013, April), 24(S1), 329Detailed reference viewed: 8 (2 ULg) Scalar triplet leptogenesis without right-handed neutrino decouplingAristizabal Sierra, Diego in Nuclear Physics B Proceedings Supplement (2013, April), 237-238We discuss leptogenesis in the context of type-II seesaw in the case in which in addition to the scalar electroweak triplet decays the lepton asymmetry is also induced by right-handed neutrino decays ... [more ▼]We discuss leptogenesis in the context of type-II seesaw in the case in which in addition to the scalar electroweak triplet decays the lepton asymmetry is also induced by right-handed neutrino decays (mild hierarchical scenarios). We show that within this setup, depending on the relative sizes of the relevant parameters, one can identify three classes of generic models, each one with its own consequences for leptogenesis. [less ▲]Detailed reference viewed: 21 (1 ULg) Effect of a single oral loading dose of 100,000 IU of cholecalciferol on phosphocalcic metabolism biomarkers in healthy adults subjectsLAURENT, Terry ; LUKAS, Pierre ; ROUSSELLE, Olivier et alin Osteoporosis International (2013, April), 24(S1), 282Detailed reference viewed: 8 (1 ULg) What is the predictive value of MRI for the occurrence of hard clinical endpoints in knee osteoarthritis?Pelletier, Jean-Pierre; Peterfy, Charles; Brandi, Maria Luisa et alin Osteoporosis International (2013, April), 24(Suppl.1), 84-85Detailed reference viewed: 9 (0 ULg) Clinical characteristics of patients responsive to whole body vibrationBeaudart, Charlotte ; Buckinx, Fanny ; Maquet, Didier et alin Osteoporosis International (2013, April), 24(Suppl.1), 243Detailed reference viewed: 39 (11 ULg) Evaluation of the impact of a 6-month training by whole body vibration on the risk of falls among nursing home residentsBeaudart, Charlotte ; Buckinx, Fanny ; Demonceau, Marie et alin Osteoporosis International (2013, April), 24(Suppl.1), 246-247Detailed reference viewed: 33 (12 ULg) Validity of the French hip and knee replacement expectations surveysNEUPREZ, Audrey ; François, Garance ; Delcour, JP et alin Osteoporosis International (2013, April), 24(Suppl.1), 374Detailed reference viewed: 38 (19 ULg) Investigation of potential new targets for the diagnosis and/or the treatment of osteoarthritisLambert, Cécile ; Dubuc, J.-E.; Montell, E. et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory ... [more ▼]Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same OA patient. We identified a large number of mediators belonging to key pathways involved in OA pathogenesis. The aim of this study was to validate different potential new targets for the diagnosis and/or the treatment OA. Methods: Synovial cells (SC) were isolated from synovial specimens obtained from OA patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. The biopsies from N/R and I areas were cultured separately for a period of 7 days. Microarray gene expression profiling between N/R and I areas was performed. The biological relevance of up- and down-regulated genes was analyzed with Ingenuity Pathways Analysis. Western blot and immunohistochemistry confirmed the identified genes most differentially expressed in the key pathways. The production of the triggering receptor expressed on myeloid cells-1 (TREM1), the alarmin S100 calcium binding protein A9 (S100A9), the wingless-type MMTV integration site family, member 5A (Wnt-5A) and the stanniocalcin 1 (STC1) were evaluated by Western blot. S100A9, hyaluronan synthase-1 (HAS1) and STC1 expression and localization were investigated by immunohistochemistry. Results: 896 genes differentially expressed in N/R and I areas were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory gene pattern, TREM1 and S100A9 were strongly upregulated. We validated the production of these proteins in OA synovial biopsies by Western blot. TREM1 and S100A9 were increased in I compared to N/R synovial cells culture. S100A9 was observed in the perivascular area and in sublining cells in I synovial biopsies, but not in N/R biopsies. An increased staining was also observed in the intima lining layer of I when compared to N/R biopsies. The most upregulated anabolism enzyme in I synovial biopsies was HAS1. Using immunohistochemistry, we observed in I areas an increase of the HAS1-positive cells mainly in the intima lining. We also studied the protein production of Wnt-5A, the most upregulated intermediate of Wnt signaling pathway. The protein level was increased in I compared to N/R areas. Finally, in the angiogenesis pathway, one the most u-regulated gene was STC1. A significant increase of STC1 production was observed in I areas compared to N/R areas by Western blot. This result was also supported by the immunohistochemical analysis. In I area, the staining for STC1 was more intense in perivascular and sublining cells. Conclusions: Synovial membrane inflammation is a key target for OA treatments. In this work, we have identified proteins involved in the synovitis pathways like angiogenesis, cells infiltration and matrix remodeling. These proteins could be targeted by drugs and used as companion biomarkers for evaluating their efficacy. Although qualitative, our results could also yield to the identification of markers of the disease. This investigation has to be further pursued. [less ▲]Detailed reference viewed: 81 (5 ULg) Role of nutraceuticals in the symptomatic and structural management of osteoarthritisBruyère, Olivier in Osteoporosis International (2013, April), 24(Suppl.1), 82-83Detailed reference viewed: 14 (0 ULg) Reproducibility of joint space width assessment when external calibration on the radiograph is missingDEROISY, Rita ; Reginster, Jean-Yves ; Bruyère, Olivier in Osteoporosis International (2013, April), 24(Suppl.1), 379Detailed reference viewed: 8 (0 ULg) Quality of life in sarcopenia and frailtyRizzoli, René; Reginster, Jean-Yves ; Arnal, Jean-François et alin Osteoporosis International (2013, April), 24(Suppl.1), 76-77Detailed reference viewed: 39 (13 ULg) Health economics in osteoarthritisHiligsmann, Mickaël ; Cooper, Cyrus; Arden, Nigel et alin Osteoporosis International (2013, April), 24(Suppl.1), 79-80Detailed reference viewed: 10 (1 ULg) Strontium ranelate improves osteoarthritis symptoms compared to placebo in patients with knee OA: The SEKOIA studyBruyère, Olivier ; Richette, P; Bellamy, N et alin Osteoporosis International (2013, April), 24(Suppl.1), 49-51Detailed reference viewed: 32 (4 ULg) OARSI recommended performance-based tests to assess physical function in people with established hip and knee osteoarthritisDobson, F.; Hinman, R.S.; Roos, E.M. et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Detailed reference viewed: 22 (1 ULg) Nonpharmacological managementBruyère, Olivier in Osteoporosis International (2013, April), 24(Suppl.1), 76Detailed reference viewed: 5 (0 ULg) Quantitative gait assessment using an accelerometer technology as a predictive tool of falls among nursing home residents: a 6-month prospective studyBruyère, Olivier ; Detalle, Anne-Sophie; Demonceau, Marie et alin Osteoporosis International (2013, April), 24(Suppl.1), 210Detailed reference viewed: 13 (4 ULg) Planet TOPERS: Planets, Tracing the Transfer, Origin, Preservation, and Evolution of Their ReservoirsDehant, V.; Van Hoolst, T.; Breuer, D. et alin Geophysical Research Abstracts (2013, April), 15An overview is given of the Planet TOPERS project addressing habitability in our solar system.Detailed reference viewed: 18 (5 ULg) Effects of chondroitin sulfate on the gene expression profile in IL-1β stimulated synovial fibroblast cells culturesLambert, Cécile ; Dubuc, Jean-Emile; Montell, E. et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control ... [more ▼]Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control and IL-1β stimulated synovial fibroblast cells cultures. In a second step, we investigated the effects of CS on this gene expression profile. Methods: OA synovial specimens were obtained from 12 patients undergoing knee replacement. At the surgery time, the synovial membrane was dissected. Synovial fibroblast cells (SFC) were enzymatically isolated and used after four passages (P4). SFC were pre-treated 1 hour with highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain) before treatment with IL-1β (1 ng/ml) for 24 hours. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class comparison test between control (Ctl) and interleukin (IL)-1β conditions, Ctl and Ctl/CS and IL-1β and IL-1β/CS conditions was based on paired t-test where Ctl and IL-1β, Ctl and Ctl/CS and IL-1β and IL-1β/CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Probes with a p-value below 0.001 were chosen and classified as up- or down-regulated ones. Results: 3308 genes were identified as differentially expressed genes between Ctl and IL-1β conditions. We observed a differential profile of expression of major pathways involved in OA pathogenesis. The key identified pathways were related to inflammation, complement cascade, angiogenesis, cartilage catabolism and anabolism and Wnt signaling. In the inflammatory network, the most upregulated cytokines were IL-8 and IL-6 with a fold change of 156.25 and 58.8 respectively. We also identified several chemokines, enzymes and metallothioneins (MTs). Complement factor B (CFB) and complement component 3 (C3) are two factors upregulated in the inflammatory complement cascade. We also identified some genes implicated in the angiogenesis pathway. The most upregulated was Stanniocalcin 1 (STC1) with a fold change of 9.09. The differential expression of intermediates involved in both cartilage anabolism and catabolism was revealed by the IL-1β stimulation, showing an imbalance in favour of catabolism. MMP-3 was largely upregulated (fold change of 62.5). Wnt 5A and low density lipoprotein receptor-related protein (LRP8) were significantly upregulated while frizzled homolog 2 (FZD2) and dickkopf homolog 3 (DKK3) were downregulated in the Wnt signaling pathway. We next performed a class comparison test between Ctl and Ctl/CS in one hand and IL-1β and IL-1β/CS on the other hand. 660 genes were identified as differentially expressed between Ctl and Ctl/CS conditions while 241 genes were identified between IL-1β and IL-1β/CS. Among them, our attention was focused on two genes upregulated in the presence of CS: lysyl oxidase-like 4 (LOXL4) and claudin 11 (CDLN11), two genes that negatively regulate cell invasion. Conclusions: We here evidenced in synovial fibroblast cells the modulation of gene expression following IL-1β stimulation. We also demonstrated the modulatory effects of CS on gene expression and isolated several CS-modulated genes of interest such as LOXL4 and CDLN11, which could constitute new mechanisms of action of the molecule and contribute to explain the symptomatic efficacy of CS in the treatment of OA. [less ▲]Detailed reference viewed: 55 (2 ULg) What is the value of biomarkers for drug development in osteoarthritis?Lotz, Martin; Martel-Pelletier, Johanne; Christiansen, Claus et alin Osteoporosis International (2013, April), 24(Suppl.1), 77-78Detailed reference viewed: 12 (2 ULg) The intra-articular injection of a new chitosan biomaterial prevents the progression of osteoarthritis in ACLT rabbit modelOprenyeszk, Frédéric ; Chausson, Mickael; Maquet, Véronique et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), 69Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on ... [more ▼]Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on cartilage lesion in osteoarthritis (OA) rabbit model. These effects were compared to those obtained with the intra-articular injection of either chitosan-based (H) hydrogel without the AC bead or saline solution. Methods OA was surgically induced by the transection of the anterior cruciate ligament (ACLT) in HYLA albino rabbits. One week after surgery, animals were randomly divided into 3 groups: group I (n=7): mix of AC beads and H hydrogel; group II (n=7): H hydrogel alone; group III (n=7): saline solution (control). The treatments (900 µl) were injected intra-articularly. X-rays from the right knee were performed before surgery, at the time of injection and at sacrifice. The standard radiographs were acquired in extension and scored by the Kellgren and Lawrence (K&L) scale. After 6 weeks, animals were euthanized and the right joint was dissected. The macroscopic evaluation of cartilage from femoral condyles and tibial plateaus stained with India ink was done. Histological sections stained with Safranine-O/fast green from bearing areas of each compartment were evaluated according to the OARSI histological score. Briefly, the evaluation considered: staining of the cartilage matrix (0-6), cartilage structure (0-11), chondrocyte density (0-4) and cluster formation (0-3), where 0 represented a normal situation and 24 points the maximum severity score. Blood samples were collected the day of injection and prior the sacrifice. Prostaglandin E2 (PGE2) and C-reactive protein (CRP) were measured in serum using immunoassays. Results The X-rays analysis showed a significant decrease (p <0.05) of the K&L score in group I (AC beads and H hydrogel; 1.5 ± 0.2) compared with group II (H hydrogel; 2.2 ± 0.5) and group III (saline solution; 3.0 ± 0.4). The size and the severity of the macroscopic OA cartilage lesion tended to decrease in group I compared to the other groups. The histological global score that refers to all compartments of the knee joint was significantly decreased in group I (11.0 ± 0.7) compared to group II (14.4 ± 0.6, p <0.01) and group III (14.8 ± 0.6, p <0.001). No significant variation of PGE2 and CRP serum levels were observed in each after 6 weeks follow-up whatever the treatment injected. Conclusions This study showed that a biphasic hydrogel composed by AC beads and H hydrogel prevented OA in rabbit with ACL transection. This effect was not observed with the hydrogel alone, suggesting that AC beads play a role in joint protection. The preventive effect was observed in all joint compartments indicating a global protective effect of this new viscosupplementation. [less ▲]Detailed reference viewed: 62 (3 ULg) Effects of chondroitin sulfate on the gene expression profile in the inflamed synovial membraneLambert, Cécile ; Dubuc, J-E; Montell, E. et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a ... [more ▼]Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a second step, we investigated the genetic modulatory effects of chondroitin sulfate (CS) in this model. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days in the absence or in the presence of highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain). Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I conditions, N/R and N/R-CS conditions and I and I-CS conditions was based on paired t-test where N/R and I, N/R and N/R-CS and I and I-CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Results: From among 47000 probes, 18253 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 465 differentially expressed genes between N/R and I areas were identified. Many inflammatory mediators appear differentially expressed. The interferon alpha-inductible protein 6 (IFI6) was the most up-regulated. We also identified the hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), the cathepsin K (CTSK), the chemokine (C-X-C motif) ligand 1 (CXCL1) and the EBV-induced G-protein coupled receptor 2 (EBI2). The differential expression of intermediates involved in angiogenesis pathway was also revealed between N/R and I areas. Among them, R-spondin-3 (RSPO3), the secreted phopshoprotein 1 (SPP1) and aquaporin 9 (AQP9) were up-regulated whereas ADAMTS1 was down-regulated. Finally, in the Wnt signaling, RSPO3 was up-regulated unlike dickkopf homolog 3 (DKK3) which was in turn down-regulated. We next performed a class comparison test between N/R and N/R-CS in one hand and between I and I-CS the other hand. 489 genes were identified as differentially expressed genes between N/R and N/R-CS conditions while 219 genes were identified between I and I-CS conditions. In this latter, our attention was focused on the down-regulated genes. Among them, we identified a number implicated in angiogenesis and cell migration pathways. Thus, the endothelial cell-specific molecule-1 (ESM1), the Transmembrane-4-L-six-family-1 (TM4SF1), the 5’-Ectonucleotidase (NT5E) and the growth arrest-specific gene 6 (GAS6) were down-regulated by CS. Conclusions: Our work demonstrates the differential gene expression profile between paired non inflammatory and normal/reactive areas of synovial membrane as well as the modulatory effects of CS on gene expression in the inflammatory areas, especially regarding genes involved in both angiogenesis and cell migration. [less ▲]Detailed reference viewed: 51 (2 ULg) Effects of chondroitin sulfate on the gene expression profile in the inflamed synovial membraneLambert, Cécile ; Dubuc, J-E; Montell, E. et alin Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a ... [more ▼]Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a second step, we investigated the genetic modulatory effects of chondroitin sulfate (CS) in this model. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days in the absence or in the presence of highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain). Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I conditions, N/R and N/R-CS conditions and I and I-CS conditions was based on paired t-test where N/R and I, N/R and N/R-CS and I and I-CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Results: From among 47000 probes, 18253 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 465 differentially expressed genes between N/R and I areas were identified. Many inflammatory mediators appear differentially expressed. The interferon alpha-inductible protein 6 (IFI6) was the most up-regulated. We also identified the hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), the cathepsin K (CTSK), the chemokine (C-X-C motif) ligand 1 (CXCL1) and the EBV-induced G-protein coupled receptor 2 (EBI2). The differential expression of intermediates involved in angiogenesis pathway was also revealed between N/R and I areas. Among them, R-spondin-3 (RSPO3), the secreted phopshoprotein 1 (SPP1) and aquaporin 9 (AQP9) were up-regulated whereas ADAMTS1 was down-regulated. Finally, in the Wnt signaling, RSPO3 was up-regulated unlike dickkopf homolog 3 (DKK3) which was in turn down-regulated. We next performed a class comparison test between N/R and N/R-CS in one hand and between I and I-CS the other hand. 489 genes were identified as differentially expressed genes between N/R and N/R-CS conditions while 219 genes were identified between I and I-CS conditions. In this latter, our attention was focused on the down-regulated genes. Among them, we identified a number implicated in angiogenesis and cell migration pathways. Thus, the endothelial cell-specific molecule-1 (ESM1), the Transmembrane-4-L-six-family-1 (TM4SF1), the 5’-Ectonucleotidase (NT5E) and the growth arrest-specific gene 6 (GAS6) were down-regulated by CS. Conclusions: Our work demonstrates the differential gene expression profile between paired non inflammatory and normal/reactive areas of synovial membrane as well as the modulatory effects of CS on gene expression in the inflammatory areas, especially regarding genes involved in both angiogenesis and cell migration. [less ▲]Detailed reference viewed: 51 (2 ULg) Orthanc - A lightweight, RESTful DICOM server for healthcare and medical researchJODOGNE, Sébastien ; Bernard, Claire ; DEVILLERS, Magali et alin Proceedings, IEEE International Symposium on Biomedical Imaging: from Nano to Macro (2013, April)Detailed reference viewed: 185 (29 ULg) Changes in the structure and the symptoms of the osteoarthritis knee and prediction of future knee replacement over an 8-year follow-up periodBruyère, Olivier ; Cooper, Cyrus; Pavelka, Karel et alin Osteoporosis International (2013, April), 24(Suppl.1), 209Detailed reference viewed: 35 (17 ULg) Red giants rotational splittingsOuazzani, R. M.; Goupil, M. J.; Dupret, Marc-Antoine et alin EPJ Web of Conferences (2013, March 01), 43The space missions CoRoT and Kepler provide high quality data that allow to test the transport of angular momentum in stars by the seismic determination of the internal rotation profile. Our aim is to ... [more ▼]The space missions CoRoT and Kepler provide high quality data that allow to test the transport of angular momentum in stars by the seismic determination of the internal rotation profile. Our aim is to test the validity of the seismic diagnostics for red giant rotation that are based on a perturbative method and to investigate the oscillation spectra when the validity does not hold. We use a non-perturbative approach implemented in the ACOR code [1] that accounts for the effect of rotation on pulsations, and solves the pulsations eigenproblem directly for dipolar oscillation modes. We find that the limit of the perturbation to first order can be expressed in terms of the core rotation and the period separation between consecutive dipolar modes. Above this limit, each family of modes with different azimuthal symmetry m, has to be considered separately. For rapidly rotating red giants, new seismic diagnostics can be found for rotation by exploiting the differences between the period spacings associated with each m-family of modes. [less ▲]Detailed reference viewed: 7 (0 ULg) Amplitudes of solar-like oscillations in red giants: Departures from the quasi-adiabatic approximationSamadi, R.; Belkacem, K.; Dupret, Marc-Antoine et alin European Physical Journal Web of Conferences (2013, March 01), 43CoRoT and Kepler measurements reveal us that the amplitudes of solar-like oscillations detected in red giant stars scale from stars to stars in a characteristic way. This observed scaling relation is not ... [more ▼]CoRoT and Kepler measurements reveal us that the amplitudes of solar-like oscillations detected in red giant stars scale from stars to stars in a characteristic way. This observed scaling relation is not yet fully understood but constitutes potentially a powerful diagnostic about mode physics. Quasi-adiabatic theoretical scaling relations in terms of mode amplitudes result in systematic and large differences with the measurements performed for red giant stars. The use of a non-adiabatic intensity-velocity relation derived from a non-adiabatic pulsation code significantly reduces the discrepancy with the CoRoT measurements. The origin of the remaining difference is still unknown. Departure from adiabatic eigenfunction is a very likely explanation that is investigated in the present work using a 3D hydrodynamical model of the surface layers of a representative red giant star. [less ▲]Detailed reference viewed: 10 (0 ULg) Non-radial, non-adiabatic solar-like oscillations in RGB and HB starsGrosjean, Mathieu ; Dupret, Marc-Antoine ; Belkacem, K. et alin EPJ Web of Conferences (2013, March 01), 43CoRoT and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. We compare the theoretical spectrum of two red giants in ... [more ▼]CoRoT and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. We compare the theoretical spectrum of two red giants in the same region of the HR diagram but in different evolutionary phases. We present here our first results on the inertia, lifetimes and amplitudes of the oscillations and discuss the differences between the two stars. [less ▲]Detailed reference viewed: 17 (9 ULg) Mode lifetime and associated scaling relationsBelkacem, K.; Appourchaux, T.; Baudin, F. et alin EPJ Web of Conferences (2013, March 01), 43Thanks to the CoRoT and Kepler spacecrafts, scaling relations (linking seismic indices and global stellar parameters) are becoming the cornerstone of ensemble asteroseismology. Among them, the relation ... [more ▼]Thanks to the CoRoT and Kepler spacecrafts, scaling relations (linking seismic indices and global stellar parameters) are becoming the cornerstone of ensemble asteroseismology. Among them, the relation between the cut-off frequency and the frequency of the maximum in the power spectrum of solar-like pulsators as well as the relation between mode lifetime and the effective temperature remain poorly understood. However, a solid theoretical background is essential to assess the accuracy of those relations and subsequently of the derived stellar parameters. We will thus present recent advances on the understanding of the underlying mechanisms governing those relations and show that the physics of mode lifetime (thus of mode damping) plays a major role. [less ▲]Detailed reference viewed: 9 (0 ULg) Stockage de bois chablis sous bâcheCastaings, Frédéric; Jourez, Benoît ; Riguelle, Simon in Infos de RND (Les) (2013, March), (1er semestre), 3-5Le samedi 24 janvier 2009, la tempête Klaus traverse le sud-ouest de la France. Tempête la plus dévastatrice depuis 1999, la forêt des Landes et ses pins maritimes paient un lourd tribut à ce phénomène ... [more ▼]Le samedi 24 janvier 2009, la tempête Klaus traverse le sud-ouest de la France. Tempête la plus dévastatrice depuis 1999, la forêt des Landes et ses pins maritimes paient un lourd tribut à ce phénomène extrême: 60% de la superficie est touchée, environ 40 millions de m3 de bois sont à terre, quatre fois la récolte annuelle. Il faudra attendre plusieurs mois avant que les aires de stockage par aspersion soient opérationnelles. Trop tard, le pin maritime montre déjà des signes de bleuissement de son bois, il est invendable sur les marchés à forte valeur ajoutée, là où l’esthétisme prime. Écarté des meilleurs débouchés, le lambris et le plancher, le manque à gagner est énorme. Un tel scénario catastrophe, notre région s’y prépare depuis 2005, tirant les enseignements des expériences étrangères et fondant son action sur les spécificités de notre forêt, de notre filière. C’est dans ce cadre que le 28 février dernier, à Eupen, des grumes d’épicéa stockées depuis presque cinq ans ont été extraites des bâches hermétiques qui les protégeaient. Comment le bois est-il ressorti de cette épreuve? Que penser de cette méthode alternative de stockage? Quelle place lui réserver ? Benoit Jourez du Laboratoire de Technologie du Bois du DEMNA (Département de l’Étude du milieu naturel et agricole) nous a apporté son éclairage et nous a conviés à l’ouverture de la poche étanche. Entre supputations et réalité des bois extraits après cinq ans, suspense… [less ▲]Detailed reference viewed: 39 (8 ULg) The empirical mass distribution of hot B subdwarfs: implications for stellar evolution theoryVan Grootel, Valérie ; Fontaine, Gilles; Charpinet, Stéphane et alin EPJ Web of Conferences (2013, March), 43Subdwarf B (sdB) stars are hot, compact, and evolved objects that form the very hot end of the horizontal branch, the so-called Extreme Horizontal Branch (EHB). Understanding the formation of sdB stars is ... [more ▼]Subdwarf B (sdB) stars are hot, compact, and evolved objects that form the very hot end of the horizontal branch, the so-called Extreme Horizontal Branch (EHB). Understanding the formation of sdB stars is one of the remaining challenges of stellar evo- lution theory. Several scenarios have been proposed to account for the existence of such objects, made of He-burning core surrounded by very thin H-rich envelope. They give quite different theoretical mass distributions for the resulting sdB stars. Detailed astero- seismic analyses, including mass estimates, of 15 pulsating hot B subdwarfs have been published since a decade. The masses have also been reliably determined by light curve modeling and spectroscopy for 7 sdB components of eclipsing and/or reflection effect binaries. These empirical mass distributions, although based on small-number statistics, can be compared with the expectations of stellar evolution theory. In particular, the two He white dwarfs merger scenario does not seem to be the dominant channel to form iso- lated sdB stars, while the post-red giant branch scenario is reinforced. This opens new questions on extreme mass loss of red giants to form EHB stars, possibly in connection with the recently discovered close substellar companions and planets orbiting sdB stars. [less ▲]Detailed reference viewed: 32 (6 ULg) Belgian multicentre experience with intestinal transplantationCeulemans, L; DE ROOVER, Arnaud ; DETRY, Olivier et alin Acta Gastro-Enterologica Belgica (2013, March), 76(1), 07Detailed reference viewed: 23 (3 ULg) Asteroseismology of hot B subdwarf starsCharpinet, Stéphane; Van Grootel, Valérie ; Brassard, Pierre et alin EPJ Web of Conferences (2013, March), 43Nonradial pulsations in Extreme Horizontal Branch stars (also known as hot B subdwarfs or sdB stars) offer strong opportunities to study through asteroseismology the structure and internal dynamics of ... [more ▼]Nonradial pulsations in Extreme Horizontal Branch stars (also known as hot B subdwarfs or sdB stars) offer strong opportunities to study through asteroseismology the structure and internal dynamics of stars in this intermediate stage of stellar evolution. Most sdB stars directly descend from former red giants and are expected to evolve straight into white dwarfs after core helium exhaustion. They thus represent the most direct link between these two stages. Their properties should therefore reflect both the outcome of the core evolution of red giant stars and the initial state for a fraction of the white dwarfs. We review the status of this field after a decade of efforts to exploit both p-mode and g-mode pulsating sdB stars as asteroseismic laboratories. From the discoveries of these two classes of pulsators in 1997 and 2003, respectively, up to the current epoch of data gathering of unprecedented quality from space, a lot of progress has been made in this area and prospects for future achievements look very promising. [less ▲]Detailed reference viewed: 22 (5 ULg) Différences d’activité de l’inflammasome NLRP3 entre sujets obèses avec et sans anomalies métaboliquesEsser, Nathalie ; L'Homme, Laurent ; DE ROOVER, Arnaud et alin Diabètes & Métabolism (2013, March), 39(suppl 1), 102Detailed reference viewed: 33 (12 ULg) An overview of white dwarf starsFontaine, Gilles; Brassard, Pierre; Charpinet, Stéphane et alin EPJ Web of Conferences (2013, March), 43We present a brief summary of what is currently known about white dwarf stars, with an emphasis on their evolutionary and internal properties. As is well known, white dwarfs represent the end products of ... [more ▼]We present a brief summary of what is currently known about white dwarf stars, with an emphasis on their evolutionary and internal properties. As is well known, white dwarfs represent the end products of stellar evolution for the vast majority of stars and, as such, bear the signatures of past events (such as mass loss, mixing phases, loss and redistribution of angular momentum, and thermonuclear burning) that are of essential importance in the evolution of stars in general. In addition, white dwarf stars represent ideal testbeds for our understanding of matter under extreme conditions, and work on their constitutive physics (neutrino production rates, conductive and radiative opacities, interior liquid/solid equations of state, partially ionized and partially degenerate envelope equations of state, diffusion coefficients, line broadening mechanisms) is still being actively pursued. Given a set of constitutive physics, cooling white dwarfs can be used advantageously as cosmochronometers. Moreover, the field has been blessed by the existence of four distinct families of pulsating white dwarfs, each mapping a different evolutionary phase, and this allows the application of the asteroseismological method to probe and test their internal structure and evolutionary state. We set the stage for the reviews that follow on cooling white dwarfs as cosmochronometers and physics laboratories, as well as on the properties of pulsating white dwarfs and the asteroseismological results that can be inferred. [less ▲]Detailed reference viewed: 28 (3 ULg) Development of an experimental device allowing plant-plant interaction studies and in situ dynamic trapping of volatile organic compounds emitted by barley (Hordeum distichon L.) rootsDelory, Benjamin ; Delaplace, Pierre ; Fauconnier, Marie-Laure et alin Communications in Agricultural and Applied Biological Sciences (2013, February 08), 78(1), 97-102In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly ... [more ▼]In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly performed on aboveground plant tissues, leaving the roles played by root VOCs in plant−plant interaction unexplored. In this context, this project aims at setting up an original experimental device allowing both dynamic trapping of VOCs emitted by mechanically damaged H. distichon roots and the study of the roles played by root VOCs in intra and interspecific plant−plant interactions. The experimental device consists of Barley seedlings cultivated in closed PTFE reactors filled with wet sand. Before being analysed by gas chromatography−mass spectrometry, root VOCs are trapped via a dynamic system on Tenax cartridges using a charcoal-filtered and humidified air. Preliminary results show that 7 day-old wounded Barley roots emit C9 fatty acid derivatives (E-non-2-enal and nona-2,6-dienal) as major compounds, contrasting with aboveground plant tissues that mainly emit C6 alcohols, aldehydes, and their derivative esters. For plant−plant interaction studies, receiver plants are exposed to an airflow enriched with VOCs from root damaged Barley plants of the same age. [less ▲]Detailed reference viewed: 129 (28 ULg) Lepton flavor violation and seesaw symmetriesAristizabal Sierra, Diego in Hyperfine Interactions (2013, February), 214When the standard model is extended with right-handed neutrinos the symmetries of the resulting Lagrangian are enlarged with a new global U(1)R Abelian factor. In the context of minimal seesaw models we ... [more ▼]When the standard model is extended with right-handed neutrinos the symmetries of the resulting Lagrangian are enlarged with a new global U(1)R Abelian factor. In the context of minimal seesaw models we analyze the implications of a slightly broken U(1)R symmetry on charged lepton flavor violating decays. We find, depending on the R-charge assignments, models where charged lepton flavor violating rates can be within measurable ranges. In particular, we show that in the resulting models due to the structure of the light neutrino mass matrix muon flavor violating decays are entirely determined by neutrino data (up to a normalization factor) and can be sizable in a wide right-handed neutrino mass range. [less ▲]Detailed reference viewed: 20 (5 ULg) Long term measurements of volatile organic compounds exchanges above a maize field at Lonzee (Belgium)Bachy, Aurélie ; Aubinet, Marc ; SALERNO, Giovanni et alin Communications in Agricultural and Applied Biological Sciences (2013, February), 78(1), 127-132VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role ... [more ▼]VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role in the atmospheric chemistry and their final impact on air pollution and climate change. Evaluation of current and future VOC emissions is thus necessary and requires a comprehensive understanding of VOC production and exchange dynamics under a wide panel of climatic conditions and ecosystems. Forest and non pastured grasslands have been largely studied for the last decade. However, knowledge about VOC fluxes from croplands remains scarce. Our study focuses on the VOC exchanges between a maize field and the atmosphere. It is incorporated in a wider project that aims to study VOC fluxes from two croplands (maize and winter wheat) and a pastured grassland. VOC fluxes have been measured on a maize field during the whole growing season using a micrometeorological method (eddy covariance). While first results show half-hourly bidirectionnal exchanges among all the preselected compounds, in average methanol stands for the greatest emitted VOC, followed by green leaf volatiles, and acetic acid is the greatest taken up VOC. Small isoprene and monoterpenes fluxes are also observed. A diurnal pattern is found for all those VOC, with greater emission/uptake during the day, suggesting a flux dependence on environmental parameters. These environmental controls will be further investigated [less ▲]Detailed reference viewed: 119 (16 ULg) Collaborations distantes synchrones dans les pratiques de conceptionBen Rajeb, Samia ; Leclercq, Pierre in International Journal of Design Sciences and Technology (2013, January)Cet article propose une analyse des pratiques de la collaboration architecturale dans des situations de travail distantes. Il s'intéresse en particulier à la mise en oeuvre d'outils pour le travail ... [more ▼]Cet article propose une analyse des pratiques de la collaboration architecturale dans des situations de travail distantes. Il s'intéresse en particulier à la mise en oeuvre d'outils pour le travail synchrones dans les agences d'architecture. Il s'appuie sur l'observations in situ de trois situations qui illustrent successivement différents modes d'interaction : nous analyserons ainsi l'usage de la visioconférence, de l'écran partagé puis du studio distant collaboratif. Pour identifier quels rôles peuvent assurer ces différents outils numériques, notre méthodologie s'appuiera sur la catégorisation proposée par les concepts architecturologiques, décrivant les opérations de conception architecturale, complétées des opérations pragmatiques de collaboration. Une grille évaluative des opérations supportées respectivement par ces trois technologies identifiera leurs limitations, atouts et conditions d'opérabilité, permettant ainsi d'apprécier la mise en oeuvre actuelle et future des technologies collaboratives en agence d'architecture. [less ▲]Detailed reference viewed: 101 (39 ULg) Erythropoietin therapy after allogeneic hematopoietic cell transplantation : a prospective randomised trial.JASPERS, Aurélie ; Baron, Frédéric ; WILLEMS, Evelyne et alin Belgian Journal of Hematology (2013, January)Based on the impairment of erythropoietin production after allogeneic hematopoietic cell transplantation (HCT), we previously reported in a phase-2 trial that recombinant human erythropoietin (rhEPO ... [more ▼]Based on the impairment of erythropoietin production after allogeneic hematopoietic cell transplantation (HCT), we previously reported in a phase-2 trial that recombinant human erythropoietin (rhEPO) therapy was very efficient when started one month after transplantation. We also demonstrated that anemia after nonmyeloabalative (NM) HCT was less sensitive to rhEPO therapy than after conventional allogeneic HCT. This prompted us to confirm these findings in a prospective randomised trial. One hundred and thirty-one patients were randomised (1:1) between no treatment (arm 1) or erythropoietin (Neorecormon) at the dose of 500 U/kg/week (arm 2). Once the target Hb (13g/dL) has been attained, the dose of rhEPO was reduced by half, while it was withheld when Hb was = 14g/dL. Cohort A included 42 patients on day 28 after myeloablative HCT, cohort B 39 patients on day 28 after NMHCT, and cohort C 50 patients on day 0 of NMHCT. Primary endpoints included proportion of complete correctors (i.e. patients reaching Hb = 13g/dL) and median time to achieve Hb correction in each arm. The proportion of complete correctors before day 126 posttransplant was 0% in group 1A vs 52.4% in group 2A, 0% in group 1B vs 69.5% in group 2B and 19.1% in group 1C vs 70.2% in group 2C. Median time to achieve Hb = 13g/dL was not reached in group 1B vs 49 days in group 2B; 363 and 59 days in groups 1A and 1B respectively and 363 and 87 days in groups 3A and 3B respectively (figure 1). Hb evolution in each group is shown in figure 2. Seventyone patients (47/62 in control groups and 24/57 in treated groups, p=0.0003) required red blood cell transfusions. The difference was most pronounced in cohort B. There was no difference in rates of thrombo-embolic events or other complications between the two arms. In conclusion, this is the first trial to demonstrate that EPO therapy hastens erythroid recovery and decreases transfusion requirements when started one month after allogeneic HCT. [less ▲]Detailed reference viewed: 25 (10 ULg) Milk components predicted by mid-infrared spectrometry as indicators of the udder health status of the dairy cowBastin, Catherine ; Laine, Aurélie ; Gengler, Nicolas in Journal of Dairy Science (2013), 96(E-Suppl. 1), Detailed reference viewed: 33 (5 ULg) Influence of circadian rhythm and PER3 genotype on executive discriminative ability under sleep deprivation during a constant routineJaspar, Mathieu ; Meyer, Christelle ; Muto, Vincenzo et alin Sleep (2013), 36Detailed reference viewed: 59 (18 ULg) Strontium ranelate prevents radiological progression in patients with primary knee osteoarthritisCooper, C; Berembaum, F; Nash, P et alin Osteoporosis International (2013), 24(1), 306-307Detailed reference viewed: 15 (2 ULg) Physico-chemical and membrane-interacting properties of D-xylose-based bolaforms. Influence of the anomeric configurationNasir, Mehmet Nail ; Legrand, Vincent; Gatard, Sylvain et alin MATEC Web of Conferences (2013), 4Sugar-based biosurfactants such as xylose-derived bolaforms have interesting properties, for example high biocompatibility and biodegradability which make them potential useful molecules in the ... [more ▼]Sugar-based biosurfactants such as xylose-derived bolaforms have interesting properties, for example high biocompatibility and biodegradability which make them potential useful molecules in the pharmaceutical and cosmetic fields. Until now, no detailed analyses of the physico-chemical properties of these compounds have been undertaken. Two symmetrical D-xylose-based bolaforms were chemically synthesized where the two xylose heads are linked via an acetal link to a hydrocarbon chain containing 18 carbon atoms and an unsaturation. The two bolaforms differ only by their anomeric configuration: or The bolaform exhibits interfacial properties at the air-water interface which is not the case for the . FTIR spectroscopy showed that the interactions between the bolaform and POPC, a model phospholipid, involve the carbonyl groups of the phospholipid [less ▲]Detailed reference viewed: 41 (20 ULg) Mise en image du spectacle et spectacularisation de l’image à l’âge baroqueDelfosse, Annick ; Dekoninck, Ralph; Delbeke, Maarten et alin Degrés : Revue de Synthèses à Orientation Sémiologique (2013)Detailed reference viewed: 89 (13 ULg) Hydrological and geopedological dynamics of a forested slopeDeraedt, Deborah ; Degré, Aurore ; Colinet, Gilles in Geophysical Research Abstracts (2013), 15Though forested watersheds are really particular in terms of hydrodynamics, most of the hydrological models oversimplify the phenomena involved. More investigations are unavoidable to improve the ... [more ▼]Though forested watersheds are really particular in terms of hydrodynamics, most of the hydrological models oversimplify the phenomena involved. More investigations are unavoidable to improve the knowledge and the modelling of this environment. Here is the aim of this study. The studied slope is located on the Houille watershed in the West of the Belgian Ardenne (50 1’47”N, 4 53’22”E) on a silty rocky soil. The site is situated under a Douglas ﬁr (Pseudotsuga menziesii (MIRB.) FRANCO) and spruce stand cover (Picea abies (L.) Karst). It is about 160 meters long with a North-West facing slope between 7 and 55%. The goal of the study is : - to characterise the hydrological and pedogeological dynamics along a forested slope, - to compare these dynamics with the tree growth. For the geopedological part of the study, eight pits were dug to describe the soil and take some soil samples used for granulometric, chemical, etc. analysis. We have used geophysical methods (Electrical Resistivity Tomography and Ground Penetrating Radar) to estimate the soil depth. As for the hydrological part of this study, moisture sensors (capacitive and TDR) have been installed in the pits along the slope. A dye tracing test has been performed to underline the preferential ﬂow and the importance of the subsurface ﬂow. Several trees have been equipped with dendrometers and some measures of the LAI and the height of the trees are planned. [less ▲]Detailed reference viewed: 44 (14 ULg) ALERT protocol: Efficiency of an e-learning training in a non-Advanced Medical Priority Dispatch System (AMPDS) Emergency Medical Services centresSTIPULANTE, Samuel ; HANSENNE, Yannick; EL-FASSI, Mehdi et alin Resuscitation (2013)Detailed reference viewed: 23 (3 ULg) ADRENAL INCIDENTALOMAS : A RETROSPECTIVE STUDY OF 115 OPERATED CASES AND ANALYSIS OF THE ACCURACY OF PREOPERATIVE DIAGNOSIS OF MALIGNANCYSimard, L; MAWEJA, Sylvie ; Meurisse, Michel et alin Surgical Endoscopy (2013), 27Detailed reference viewed: 17 (4 ULg) Self-shuffling wordsCharlier, Emilie ; Kamae, Teturo; Puzynina, Svetlana et alin Lecture Notes in Computer Science (2013)In this paper we introduce and study a new property of infinite words which is invariant under the action of a morphism: We say an infinite word x, defined over a finite alphabet A, is self-shuffling if x ... [more ▼]In this paper we introduce and study a new property of infinite words which is invariant under the action of a morphism: We say an infinite word x, defined over a finite alphabet A, is self-shuffling if x admits factorizations: x=\prod_{i=1}^\infty U_iV_i=\prod_{i=1}^\infty U_i=\prod_{i=1}^\infty V_i with U_i,V_i \in \A^+. In other words, there exists a shuffle of x with itself which reproduces x. The morphic image of any self-shuffling word is again self-shuffling. We prove that many important and well studied words are self-shuffling: This includes the Thue-Morse word and all Sturmian words (except those of the form aC where a is a letter and C is a characteristic Sturmian word). We further establish a number of necessary conditions for a word to be self-shuffling, and show that certain other important words (including the paper-folding word and infinite Lyndon words) are not self-shuffling. In addition to its morphic invariance, which can be used to show that one word is not the morphic image of another, this new notion has other unexpected applications: For instance, as a consequence of our characterization of self-shuffling Sturmian words, we recover a number theoretic result, originally due to Yasutomi, which characterizes pure morphic Sturmian words in the orbit of the characteristic. [less ▲]Detailed reference viewed: 26 (8 ULg) Potential of mid-infrared spectrum of milk to detect changes in the physiological status of dairy cowsLaine, Aurélie ; Goubau, Amaury; Hammami, Hedi et alin Journal of Dairy Science (2013)Detailed reference viewed: 24 (12 ULg) Strontium ranelate effect on knee osteoarthritis progression : a MRI analysisGenant, HK; Zaim, S; Guermazi, A et alin Osteoporosis International (2013), 24(1), 312-313Detailed reference viewed: 25 (2 ULg) Biofilm formation on metal structured packing for the production of high added value biomoleculesZune, Quentin ; Zune, Quentin ; Toye, Dominique et alin Récents Progrès en Génie des Procédés (2013)Many white biotechnology bioprocesses apply techniques from chemical engineering based on bioreactors with mechanical stirring system commonly employed in pharmaceutical sector, food industry or energy ... [more ▼]Many white biotechnology bioprocesses apply techniques from chemical engineering based on bioreactors with mechanical stirring system commonly employed in pharmaceutical sector, food industry or energy field (Dasilva, 2004). As in chemical engineering, scale-up of these bioprocesses induces physicochemical constraints that affect physiological pathways and decrease performances. In this context, it is essential to think new bioprocesses better suited to physiology of microorganisms, minimizing physicochemical constraints. The aim of this work consists to use stainless steel structured packing (SSP) with high specific area (500-750 m²/m³) as inert support for biomass immobilization in order to produce high added value biomolecules. These bioreactors are biocatalysts in which microbial system is immobilized biomass on the form of a biofilm performing bioconversion of a substrate into a specific product (Rosche, 2009). In this study, an experimental setting containing a SSP reproduces solid-state fermentation (SSF) like conditions. Two well known microorganisms for their ability to form biofilm and secrete metabolites are tested in the experimental setting : Bacillus subtilis for its lipopeptides and Aspergillus oryzae for its glucoamylase. Effectiveness of the bioprocess in term of dynamic of the excretion of the target biomolecule is compared with a classical submerged culture (SmF). For lipopeptides production from B. subtilis, SSP is located in a 20L bioreactor continuously aspersed by liquid medium required to the growth of the biofilm. In the case of A. oryzae, the SSP is partially immerged in a 250 mL shake flask. X-ray tomography of the SSP allows non-invasive visualization and quantification of biofilm repartition inside the support. Implementation of SSP permits almost total immobilization of biomass on the form of a mono-species biofilm to the detriment of the liquid phase. Processing of images obtained by X-ray tomography of the SSP provides relevant information for the optimization of the bioprocess. For both microorganism species, results indicate the influence of parameters such as hydrodynamics, aeration rate and microorganism specificity, on the biofilm morphology inside the support and the performances of the bioprocess. SSF-like conditions in the experimental setting lead to technologic progress, such as absence of foam formation, persistence of the microbial system, and improve the dynamic of metabolites excretion compared with conditions imposed by the submerged culture. Further experiment will consider hydrodynamics aspects and amount of carbon source on effectiveness of the bioprocess. [less ▲]Detailed reference viewed: 75 (28 ULg) Imaging osteoarthritis using (18F)FPRGD2 PET/CT : observation and potential application.WITHOFS, Nadia ; ALVAREZ MIEZENTSEVA, Victoria ; SIMONI, Paolo et alin Journal of Nuclear Medicine (The) (2013), 54(SUPPL), 250Detailed reference viewed: 4 (1 ULg) Phenomenology of $\alpha_s$ at intermediate energy: the quark-hadron duality approachCourtoy, Aurore in PoS - Proceedings of Science (2013)Detailed reference viewed: 16 (1 ULg) Prognostic value of FDG PET/CT in patients with hepatocellular carcinoma treated with liver transplantation.GOVAERTS, L.; DETRY, Olivier ; BLETARD, Noëlla et alin European Journal of Nuclear Medicine and Molecular Imaging (2013), 2013(SUPPL), 287Detailed reference viewed: 2 (0 ULg) Prognostic value of FDG PET/CT in patients with lung tumors treated by Cyberknife.LOVINFOSSE, Pierre ; JANVARY, Zsolt Levente ; JANSEN, Nicolas et alin Journal of Nuclear Medicine (The) (2013), 54(SUPPL), 567Detailed reference viewed: 4 (0 ULg)