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See detailRéaction inflammatoire exubérante comme effet secondaire d’une infiltration de PRP
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; LEONARD, Philippe ULg et al

in Annals of Physical and Rehabilitation Medicine (2013, October), 56(Sup 1), 068

Introduction : Infiltrations of plasma rich platelets (PRP) represent a new treatment of tendinopathies. Currently, no side effects were reported in this indication. Case report: We report the case of a ... [more ▼]

Introduction : Infiltrations of plasma rich platelets (PRP) represent a new treatment of tendinopathies. Currently, no side effects were reported in this indication. Case report: We report the case of a 35-year-old type 1 diabetic patient with right upper patellar tendinopathy that had persisted for more than 6 months. The patient benefited from an intratendinous infiltration of 6 mL of PRP (8.105 platelets/mm3, almost no red or white blood cells) after a carefully disinfection but without local anesthesia. Typically, a standardized program of sub-maximal eccentric rehabilitation should be started 1 week after infiltration. However, the patient experienced local swelling with erythema, increased heating and pain, which appeared just underneath the patella, without biological inflammatory syndrome. In absence of septic general symptoms, no blood or wound culture were made. At 2 weeks post-infiltration, a greatly increased Doppler signal in a thicker tendon was observed by ultrasounds compared to that before infiltration, but there was no sign of infection demonstrated by either MRI or CT. However, the local inflammation did not decrease after a 3-week treatment of local cryotherapy, local and oral NSAID, and adjunct use of colchicine 1 mg. Thus, an insidious infection was suspected, even though there was no evidence of biological inflammatory syndrome or sign of infectious lesion on imagery examination. Antibiotic therapy (rifampicine 600 mg + minocycline 100 mg), was initiated for three months. Due to a lack of improvement via imaging and clinical examination, a 3-phase bone scintigraphy was performed. The results suggested the presence of a complex regional pain syndrome type 1. The patient benefited from classical physical therapy and concomitant pain killers. The evolution was favorable after 6 months of treatment. Discussion : Even though PRP infiltration represents a new and promising treatment for tendinopathy, more studies are needed both to verify its clinical efficacy. Moreover, implementing this innovative treatment requires caution because of potential adverse events. Thus, the balance between benefits and risks must be carefully evaluated before using this treatment, especially in patients with type 1 diabetes. [less ▲]

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See detailElaboration de critères pour l'évaluation de "l'agir" du plan national nutrition santé en Belgique
Absil, Gaetan ULg; Schippers, Nathalie ULg; Vandoorne, Chantal ULg

in Revue d'Epidémiologie et de Santé Publique = Epidemiology and Public Health (2013, October), 61(4), 304

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See detailPratique des actes préventifs : ce qui détermine l'attitude des médecins généralistes belges
Vanmeerbeek, Marc ULg

in Revue d'Epidémiologie et de Santé Publique = Epidemiology and Public Health (2013, October), 61(octobre), 309

En Belgique, l’implémentation des procédures préventives reste insuffisante dans plusieurs domaines, avec un gradient socio-économique défavorable aux moins nantis. Une implication accrue des médecins ... [more ▼]

En Belgique, l’implémentation des procédures préventives reste insuffisante dans plusieurs domaines, avec un gradient socio-économique défavorable aux moins nantis. Une implication accrue des médecins généralistes pourrait améliorer ces deux aspects, grâce à leur proximité avec les bénéficiaires potentiels. L’opinion des généralistes belges sur un investissement accru en prévention a été évaluée au moyen d’une échelle d’attitude construite au départ de verbatims provenant d’une enquête qualitative préalable. L’échelle a exploré 5 axes : vision globale de la santé, gestion collective des patients, évaluation de processus, collaboration et santé publique. Un questionnaire postal a été envoyé à 2.500 généralistes, dont 457 ont répondu valablement. L’axe gestion collective a obtenu la moyenne des scores d’attitude favorable à la prévention la plus élevée (77%), devant les axes santé globale (76%), évaluation (72%), collaboration (69%) et santé publique (63%). L’analyse factorielle a révélé deux facteurs principaux, l’organisation de la pratique (62% de la variance) et la formalisation (14% de la variance). L’organisation de la pratique (gestion des échéances, des données, pro-activité, sentiment de responsabilité envers la patientèle) était le facteur principal et était lié surtout aux associations et groupes pluridisciplinaires. La formalisation (codification précise de la pratique, assortie ou non d’une évaluation) venait ensuite et expliquait les plus fortes différences entre praticiens et entre communautés linguistiques. Les meilleurs scores étaient liés à une moindre ancienneté (<20 ans de pratique), aux associations et à la pratique urbaine. Le sexe du médecin ou le fait de travailler dans une structure préventive comme la protection maternelle et infantile étaient sans effet significatif. L’organisation de la prévention dépend des gouvernements de Flandre et de Wallonie-Bruxelles, mais des facteurs personnels et organisationnels propres aux généralistes doivent être considérés pour expliquer les résultats actuels. Une diffusion plus large et équitable des actes préventifs passe par la prise en compte de ces facteurs. [less ▲]

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See detailGerminome intracrânien bifocal : la biopsie est-elle toujours indispensable?
KREUTZ, Julie ULg; BONNEVILLE, Jean-François ULg; Potorac, Iulia ULg et al

in Annales d'Endocrinologie (2013, October), 74

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See detailEvaluation de l’implantation de la vaccination contre le papillomavirus dans les pratiques des services de santé scolaire en Fédération Wallonie Bruxelles
Miermans, Marie-Christine ULg; Swennen, Béatrice; Vermeeren, Axelle

in Revue d'Epidémiologie et de Santé Publique = Epidemiology and Public Health (2013, October), 61(Supplément 4), 233

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See detailCoexistence entre adénom hypophysaire et phéochromocytome - présentation de cas
Rostomyan, Liliya ULg; Potorac, Iulia ULg; Filipponi, S et al

in Annales d'Endocrinologie (2013, October), 74

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See detailLe carcinome parathyroïdien familial : une forme agressive d'hyperparathyroïdie primaire
Tudorescu, A; VROONEN, Laurent ULg; BETEA, Daniela ULg et al

in Annales d'Endocrinologie (2013, October), 74

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See detailHypothyroïdie infraclinique non auto-immune et statut iodé : étude prospective d'intervention
VALDES SOCIN, Hernan Gonzalo ULg; Tudorescu, A; Lutteri, L et al

in Annales d'Endocrinologie (2013, October), 74

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See detailCorrélations significatives de l'aspect en IRM haute résolution des adénomes hypophysairesà GH avant traitement
Potorac, Iulia ULg; PETROSSIANS, Patrick ULg; Schillo, F et al

in Annales d'Endocrinologie (2013, October), 74

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See detailUne néoplasie endocrinienne multiple particulière
Boccar, S; VROONEN, Laurent ULg; HAMOIR, Etienne ULg et al

in Annales d'Endocrinologie (2013, October), 74

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See detailTRAPPIST (TRAnsiting Planets and PlanetesImals Small Telescope)
Jehin, Emmanuel ULg; Gillon, Michaël ULg; Opitom, Cyrielle ULg et al

in EPSC Abstract 2013 (2013, September 13), 8

TRAPPIST is a 60-cm robotic telescope that has been installed in June 2010 at the ESO La Silla Observatory [1]. Operated from Liège (Belgium) it is devoted to the detection and characterisation of ... [more ▼]

TRAPPIST is a 60-cm robotic telescope that has been installed in June 2010 at the ESO La Silla Observatory [1]. Operated from Liège (Belgium) it is devoted to the detection and characterisation of exoplanets and to the study of comets and other small bodies in the Solar System. We describe here the hardware and the goals of the project and give an overview of the comet production rates monitoring after three years of operations. [less ▲]

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See detailCould bonobos, Pan paniscus, influence forest re(colonization) in a forest-savana mosaic?
Trolliet, Franck ULg; Serckx, Adeline ULg; Huynen, Marie-Claude ULg et al

in Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013, September 12)

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See detailTRAPPIST monitoring of comet C/2012 F6 (Lemmon)
Opitom, Cyrielle ULg; Jehin, Emmanuel ULg; Manfroid, Jean ULg et al

in EPSC Abstract 2013 (2013, September 12), 8

Comet C/2012 F6 is a long-period comet that reached perihelion on March 23, 2012. The unexpected brightness of this comet since December 2012 allowed us to obtain narrowband photometry and to study its ... [more ▼]

Comet C/2012 F6 is a long-period comet that reached perihelion on March 23, 2012. The unexpected brightness of this comet since December 2012 allowed us to obtain narrowband photometry and to study its chemical composition as well as its rotation. [less ▲]

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See detailStudy of the forbidden oxygen lines in comets at different heliocentric and nucleocentric distances
Decock, Alice ULg; Jehin, Emmanuel ULg; Rousselot, Philippe et al

in EPSC Abstract 2013 (2013, September 12), 8

Oxygen is an important element in the chemistry of the Solar System objects given its abundance and its presence in many molecules including H2O, which constitutes 80% of cometary ices. The analysis of ... [more ▼]

Oxygen is an important element in the chemistry of the Solar System objects given its abundance and its presence in many molecules including H2O, which constitutes 80% of cometary ices. The analysis of oxygen atoms in comets can provide information not only on the comets themselves but also on our Solar System. These atoms have been analyzed using the three forbidden oxygen lines [OI] observed in emission in the optical region at 5577 Å (the green line), 6300 Å and 6364 Å (the red lines) [1]. These lines are difficult to analyze because their detection requires high spectral and spatial resolutions. The oxygen analysis is interesting because it allows the determination of its parent molecules. [less ▲]

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See detail"Product-oriented engineering" applied to the development of porous scaffolds for tissue engineering.
de Bien, Charlotte ULg; Ounally, Thameur ULg; Collard, Valérie ULg et al

in Procedia Engineering (2013, September 02)

This work applies a “product-oriented engineering” approach to the development of porous scaffolds for tissue engineering. Polylactic acid scaffolds were obtained by a freeze-drying process. Their 2D/3D ... [more ▼]

This work applies a “product-oriented engineering” approach to the development of porous scaffolds for tissue engineering. Polylactic acid scaffolds were obtained by a freeze-drying process. Their 2D/3D microstructure was characterized by X-ray microtomography and related to the operating conditions used. Two types of scaffold end-use properties were characterized in relation to their microstructure: the mechanical and the transport properties. Scaffold young's modulus was computed from data measured on tension-compression devices. To evaluate the permeability, Darcy’s experiments were carried out. The obtained results allowed highlighting the quantitative relationships existing between elaboration conditions, microstructure and end use properties of the fabricated PLA scaffolds. [less ▲]

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See detailShoulder and handball
Gleizes Cervera, S.; Croisier, Jean-Louis ULg; Kaux, Jean-François ULg et al

in European Journal of Sports Medicine (2013, September), 1(1),

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See detailImmunohistochemical transforming growth factor Beta1 and its activation, storage and signalling pathways in canine idiopathic pulmonary fibrosis
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailInfluence of concentration on equine fresh semen conservation
Ponthier, Jérôme ULg; Bloomaert, Didier; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 85

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See detailCritères de retour sur le terrain après plastie LCA chez le footballeur professionnel
Croisier, Jean-Louis ULg; Rochcongar, Pierre; Bruyère, Olivier ULg et al

in European Journal of Sports Medicine (2013, September), 1(1),

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See detailIMMUNOHISTOCHEMICAL ANALYSIS OF TRANSFORMING GROWTH FACTOR BETA 1 AND ITS SIGNALLING PATHWAYS IN CANINE IDIOPATHIC PULMONARY FIBROSIS
Krafft, Emilie ULg; Laurila, HP; Rajamäki, MM et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailIntérêt des ondes de choc dans le traitement des fasciites plantaires
Fromm, Aurélien; Vanderthommen, Marc ULg; LAURENT, Jean-Marie ULg et al

in European Journal of Sports Medicine (2013, September), 1(Supplement 1), 276-277

Introduction : Over the past ten years, radial shock wave therapy usage has grown tremendously. Prescribed for chronic tendinopathy, its real benefit has yet to be demonstrated. Objective : Demonstrate ... [more ▼]

Introduction : Over the past ten years, radial shock wave therapy usage has grown tremendously. Prescribed for chronic tendinopathy, its real benefit has yet to be demonstrated. Objective : Demonstrate radial shock wave therapy benefits in plantar fasciitis treatment. Materials and methods : Our experimentation is a study over twelve weeks divided in 2 periods: the control phase (week 0 to week 6) and treatment phase (week 6 to week 11). It includes 3 evaluations (T1, T2 and T3) respectively at 0, 6 and 11 weeks. A standard evaluation consists of one VAS pain, an algo-functional backwash survey about the ankle (modified FFI), algometer test and dynamic analysis of the foot on strength platform. The radial shock wave therapy has been performed thanks to Swiss DolorClast machine (Electro Medical Systems) Results : Statistical analysis show radial shock wave therapy induces a highly significant improvement (p<0,0001) on EVA results (50%), ankle algo-functional backwash (62.6%) and pain sensitivity threshold (49.6%). Therefore, a significant correlation can be observed during T2- T3 period between pain sensitivity threshold improvement and patients age (p=0,012). The statistical results show a time increase trend for foot-ground contact over time. It is not significant though. Conclusion : The use of radial shock wave therapy in plantar fasciitis treatment helps decrease pain and improve step. However, additional studies performed on bigger samples have to be completed to confirm these results. [less ▲]

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See detailRéaction inflammatoire exubérante suite à une infiltration de PRP
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; LEONARD, Philippe ULg et al

in European Journal of Sports Medicine (2013, September), 1(Supplement 1), 278-279

Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively ... [more ▼]

Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively new treatment for tendinopathies. To date, no side effects have been reported after infiltration of PRP to treat tendinopathy. Case report: A 35-year-old patient had a right upper patellar tendinopathy which was resistant to all conservative treatments for more than 6 months. The patient was a type 1 diabetic (well controlled). He had an intratendinous infiltration of 6 mL of PRP (8.105 platelets/mm3, almost no red or white blood cells) after disinfection but without local anaesthetic. Immediately following the infiltration, local cryotherapy was performed for 15 minutes. NSAIDs were avoided, but class-1 or -2 pain-killers were authorised if necessary. A standardised sub-maximal eccentric rehabilitation should have been started 1 week after. However, the patient experienced local swelling with erythema, increased heating and pain which appeared just underneath the patella, but without biological inflammatory syndrome. A great Doppler signal in a thicker patellar tendon was observed by US, but there was no sign of local infectious disease demonstrated by either CT or MRI. However, the local inflammation did not decrease after a progressive 3-week treatment of local cryotherapy, local and oral NSAIDs and colchicine 1 mg. Thus, an insidious infection was suspected, even though there was neither evidence of biological inflammatory syndrome nor sign of infectious lesion on imagery examination. An antibiotic therapy (rifampicine 600 mg + minocycline 100 mg) was initiated for 3 months. Finally, a 3-phase bone scintigraphy suggested the presence of a complex regional pain syndrome type 1 treated by a classical physical therapy and concomitant class-2 pain killers. The evolution was favourable after 6 months of symptomatic treatment, and the pain decreased to a level similar to that before the infiltration of PRP. Discussion/Conclusions: This case report draws attention to potential side effects that are linked to this new therapy by infiltration of platelet rich plasma in case of tendinopathy, in particular when used in patients with type 1 diabetes. Thus, the balance between benefits and risks must be carefully evaluated before using this treatment in patients with type 1 diabetes. [less ▲]

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See detailHazard factors of ACL rupture: Neuromuscular factors
Kaux, Jean-François ULg; Delvaux, François ULg; MASSART, Nicolas ULg et al

in European Journal of Sports Medicine (2013, September), 1(supplement 1), 50-51

Injuries to the anterior cruciate ligament (ACL) of the knee are disabling, often associated with other intra-articular damages and increase the risk of early onset of osteoarthritis. It is very probable ... [more ▼]

Injuries to the anterior cruciate ligament (ACL) of the knee are disabling, often associated with other intra-articular damages and increase the risk of early onset of osteoarthritis. It is very probable that multiple risk factors act in combination to influence injury risk. It is important to have a comprehensive understanding of these ACL risk factors, whose neuromuscular factors, even if investigations on neuromuscular factors reported to date do not provide a complete understanding of ACL injury risk. According to several recent studies, the neuromuscular control of joint biomechanics during a specific activity seems to represent a predicting factor of an ACL injury, by quantifying the intersegmental forces and moments generated about the tibio-femoral joint. Laboratory studies have shown that landing from a jump performs cutting and pivoting maneuvers with less knee and hip flexion, increases knee valgus and internal rotation of the hip coupled, with increased external rotation of the tibia and quadriceps muscle activation (especially in women). It has been hypothesized that these movement patterns increase the strain in the ACL during activity and that the large difference in knee injury incidence rates between males and females (1/4.5) may be attributed to neuromuscular differences and resultant mechanics. Although studies have shown that the position of the knee and the magnitude and sequence of muscle contraction can increase ACL strain values, it is hard to exactly correlate these movements to what occurs during activity and sport and at the time of ACL injury. Recently, a simpler assessment tool has been validated and is able to be administered in a clinic-based testing environment Consequently, the screening for ACL injury risk could be performed on a more widespread population. Athletes who went on to a primary ACL injury also demonstrated significant side to side differences in lower extremity biomechanics as well as reduced relative lower extremity flexor activation relative to an uninjured control population during the vertical drop jump. Similar mechanisms of injury risk have been identified in athletes medically cleared to return to sport after ACL reconstruction. These seminal findings indicate that these abnormal and asymmetrical biomechanical and neuromuscular control profiles are likely both residual to, and exacerbated by, the initial injury. A study revealed that a fatigue-induced protocol altered the latency as well as the magnitude of reflex responses of the hamstring muscles and the tibial translation only in women. The authors of various studies have suggested that the hamstring muscles play an important role in maintaining knee stability and that they protect the ACL during movements of the tibia relative to the femur. Therefore, decreased reflex responses of the hamstring muscles and in turn an increased the tibial translation might contribute to the pathomechanics of the ACL injuries. It is therefore conceivable that the fatigue-induced decrease of the hamstring neuromuscular function may increase the tibial translation and probably contributes to the higher incidence of ACL injuries, especially in women. A preventive approach to decrease ACL injuries could integrate muscle imbalances as a risk factor. If it has been scientifically validated than the muscle strength profile determined by an isokinetic testing offers a predictive value on the hamstring lesion occurrence, similar studies have not permitted such a conclusion about ACL injury. The isokinetic assessments after ACL reconstruction have allowed us to observe, on the healthy contralateral knee, a higher frequency of reduced hamstring/quadriceps ratios. A possible pre-existing weakness in the hamstring and the occurrence of an ACL injury is therefore possible but only a difficult prospective approach due to the multifactorial nature of ligament injuries could clarify that point. In conclusion, a functional analysis of the landing of a jump and an isokinetic muscle strength assessment have been suggested to represent predictive elements of an ACL rupture, but further studies are needed to have a stronger evidence of their predictive qualities of injury. [less ▲]

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See detailRunning races: study of the stress and cardiac biomarkers
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; MELON, Pierre ULg et al

in European Journal of Sports Medicine (2013, September), 1(Supplement 1), 212

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured ... [more ▼]

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured by new commercially available high-sensitive methods in subjects undergoing a marathon and an ultra-trail. Our aim was to compare cTnT and NT-proBNP levels before and after the stress tests, in sportive subjects. Materials and Methods: Twenty eight subjects (26 men, 42.5 ± 11 yo) ran the Maasmarathon (42.195 kilometers) between Visé (Belgium) and Maastricht (The Netherlands) and 33 subjects (33 men, 45.7±9.3yo) ran the Ultratour of Liège (Belgium ; 67 km). We took blood sample before (T0), just after (T1) and 3 hours after the race (T3). In all the patients, cTnT concentrations were measured by high sensitive methods (hsTnT, Roche Diagnostics) on heparin plasma. The NT-proBNP was also determined with the kit Roche on heparin plasma. The protocol was approved by the Ethics Committee of the University of Liège (Belgium). All subjects gave their informed consent. All statistical analyses were performed using Medcalc version 8.1 for Windows. p-value <0.01 was regarded as statistically significant. Results and discussion: A significant difference between hsTnT concentrations at T0 and T1 (p<0.0001), and between T0 and T3 (p<0.001) for NT-proBNP have been observed, but not between T1 and T3. This observation appeared only after a strenuous exercise. However, up to now this type of exercise is not reproducible easily in a laboratory. Moreover, nobody knows if these observations would have cardiac consequences at long terms. Conclusions: Measurement of cardiac troponins by high sensitive methods allows detecting significant release of biomarkers from the heart during exercise. The value of NT-proBNP are also significant but less than TnThs. We think that the TnThs could be an interesting tool in the future to help sport medicine to detect risk of developing a cardiac problem in the future or a sudden death. [less ▲]

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See detailPhysiological interpretation of the slope during an isokinetic fatigue test of the knee
Bosquet, L.; Gouadec, K.; Berryman, N. et al

in European Journal of Sports Medicine (2013, September), 1(1), 151

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See detailIs the CXC-Chemokine CXXL8 involved in the breed predisposition of west highland white terrier to canine idiopathic pulmonary fibrosis ?
Roels, Elodie ULg; Krafft, Emilie ULg; Laurila, HP et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailImpacts of WLG on the regeneration of logged forests: preliminary insights in a Gabonese logging concession
Haurez, Barbara ULg; Petre, Charles-Albert ULg; Doucet, Jean-Louis ULg

in Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013, September), 84(3-5), 284-285

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See detailIs 5-Hydroxytryptamine (serotonin) involved in the pathogenesis of idiopathic pulmonary fibrosis in dogs ?
Krafft, Emilie ULg; Roels, Elodie ULg; Heillilä, HP et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailA case of Longitudinal Vaginal Septum in a mare
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 112-113

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport ... [more ▼]

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport performance. It is most likely under-diagnosed as it is often asymptomatic and an incidental finding. [less ▲]

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See detailEvaluation of Everolimus (EVE) in HER2+ advanced breast cancer (BC) with activated PI3K/mTOR pathway : exploratory biomarker observations from the BOLERO-3 trial
JERUSALEM, Guy ULg; ANDRE, Fabrice; CHEN, David et al

in European Journal of Cancer (2013, September), 49(Supplement 3), 8

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See detailLes effets d'une supplémentation en vitamine D sur la force musculaire : une méta-analyse d'essais randomisés contrôlés
Beaudart, Charlotte ULg; Buckinx, Fanny ULg; Rabenda, Véronique ULg et al

in Cahiers de l'Année Gérontologique (Les) (2013, September), 5(1-2), 38

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See detailGP’s engagement in detecting and managing abuse of alcohol, illegal drugs, hypnotics and tranquilizers in the Belgian adult population
Ketterer, Frédéric ULg; Lambrechts, Marie-Claire; Symons, Linda et al

in European Journal of General Practice (2013, September), 19(3), 162-184

Background General practitioners (GPs) Occupational physicians (OPs) can play an important role in detecting and managing substance abuse in the working population. The UP TO DATE project aims at ... [more ▼]

Background General practitioners (GPs) Occupational physicians (OPs) can play an important role in detecting and managing substance abuse in the working population. The UP TO DATE project aims at identifying the difficulties these professionals encounter in this area, and to explore ways of collaboration in providing appropriate care. Research question What are the experiences, attitudes and decision making of GPs and OPs regarding to alcohol, illegal drugs, hypnotics and tranquilizers abuse from a physician’s perspective? Method In this qualitative study, with a phenomenological perspective, 20 GPs and 16 OPs, experienced with substance abuse in daily practice, got a face to face in-depth interview. (October until December 2012). Along with the data collection process the analysis started with a constant comparison between the data and the chosen integrated model of change (De Vries), using coding techniques of grounded theory. Results GPs meet important barriers to detect misuse of all substances. To address this issue and the patient’s motivation for change, the doctor-patient relationship is crucial. The risk to disturb the relationship, and loose the patient’s trust, is a major concern. An attitude of patient-centeredness, collaboration and empowerment of the patient is needed to make any progress, to get results and to overcome as a GP the burden of those demanding encounters. Self-care is an important concern. Collaboration with specialised health providers confronted GPs with various problems: not enough services, long waiting lists, unclear methods and criteria. GPs lack an insight in the OP’s role, and experience problems to contact them. Whether the OP is a trustful health advocate for their patient is a major concern. Some OPs are perceived too linked to the employer, raising legitimate concerns about professional confidentiality. Conclusion These are preliminary results. At the conference, a comprehensive overview on the qualitative analysis will be given. [less ▲]

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See detailImportance du déficit en vitamine D chez les femmes françaises ostéoporotiques et ostéopéniques âgées de plus de 80 ans
Bruyère, Olivier ULg; Slomian, Justine ULg; Beaudart, Charlotte ULg et al

in Cahiers de l'Année Gérontologique (Les) (2013, September), 5(1-2), 75

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See detailHypercalcémie réfractaire et sécrétion ectopique de calcitonine dans un cancer neuroendocrine du pancréas : Effets hypocalcémiants du Cinacalcet
VALDES SOCIN, Hernan Gonzalo ULg; LOLY, Jean-Philippe ULg; BETEA, Daniela ULg et al

in Annales d'Endocrinologie (2013, September), 74(4), 235-462

Introduction: Paraneoplastic hypercalcemia is a sign of poor prognosis, as it is particularly resistant to the usual hypocalcemic treatments. Observation: In 2009, a well differentiated pancreatic ... [more ▼]

Introduction: Paraneoplastic hypercalcemia is a sign of poor prognosis, as it is particularly resistant to the usual hypocalcemic treatments. Observation: In 2009, a well differentiated pancreatic neuroendocrine tumor (Ki-67= 2%) is diagnosed in a 52-year-old diabetic man. The tumor is revealed with a splenic and hepatic carcinomatosis. Plasmatic calcium was: 3.54 mmol/L (2.15 - 2.6). Biology showed hypophosphatemia, PTH < 4 ng/ml, high 1-25 OH VitD, calcitonin: 1016 ng/ml (< 12 ng/ml). He had hypercalciuria and hypophosphaturia. He received for two years several cycles of Streptozotocin-ADRIAMYCIN and FOLFOX, with partial control of the tumor mass and calcium levels. In 2012, calcitonin levels are 29 ng/ml whereas calcemia is 3.17 mmol/L. Hypercalcemia is refractory to hyperhydration, diuretics, corticosteroids, and bisphosphonates therapy. Cinacalcet (Mimpara) is prescribed up to 120 mg/day (PO). Calcemia decreases gradually from 3 to 2.87 and then 2.76 mmol/L. PTH and calcitonin-the tumor mass remain unchanged. After two months of Cinacalcet treatment, Sunitinib (Sutent) 37.5 mg per day was added. During the third month, calcium levels dropped to 2.09 mmol/L and PTH raised to 78 pg/ml, requiring discontinuation of Mimpara. Calcitonin normalized, with a further improvement over pancreatic and metastatic lesions. Conclusion: Cinacalcet is a Calcium Sensing Receptor oral agonist. Cinacalcet hypocalcemic effects have not been previously documented in pancreatic paraneoplastic hypercalcemia. In our patient, Cinacalcet has significantly improved cancer prognosis: this drug could be a new alternative in paraneoplastic hypercalcemia. [less ▲]

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See detailInfluence of game playing on the muscular profile of shoulder rotators of handball players
Ucay, O.; Gasq, D.; Forthomme, Bénédicte ULg et al

in European Journal of Sports Medicine (2013, September), 1(1), 105

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See detailPyovagina caused by pyometra and a persistent hymen in a 9-year-old bitch
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 114

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least ... [more ▼]

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least partially- perforated hymen. For an unknown cause the hymen must have re-sealed after uterine contamination explaining a pyovagina that was bulging in the perineal area [less ▲]

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See detailComparison of four cartridge type automated blood gas analysers for arterial blood gas analysis in dogs
Roels, Elodie ULg; Delvaux, François ULg; Farnir, Frédéric ULg et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailCaractéristiques cliniques des sujets répondeurs à la vibrotonie corporelle totale
Beaudart, Charlotte ULg; Buckinx, Fanny ULg; Maquet, Didier ULg et al

in Cahiers de l'Année Gérontologique (Les) (2013, September), 5(1-2), 118-119

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See detailEffect of the lengthening of the protocol on the reliability of knee muscle fatigue indicators
Bosquet, L.; Maquet, Didier ULg; Forthomme, Bénédicte ULg et al

in European Journal of Sports Medicine (2013, September), 1(1), 150

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See detailPrimate Behaviour and Parasite Transmission in the Ecuadorian Amazon
Martin, Sarah ULg; Carrillo Bilbao, Gabriel Alberto; Bernstein, Sophie et al

in Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013, August 10), 84(3-5), 135-346

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See detailA Methodology for The Appropriation And The Isolation of Nodal Diameter Modes in Cyclic Symmetric Structures
Nyssen, Florence ULg; Viguié, Régis; Simon, Daniel et al

in Proceedings of ASME 2013 International Design Engineering Technical Conference & Computers and Information in Engineering Conference IDETC/CIE 2013 (2013, August)

This paper presents a methodology enabling the appropriation and the isolation of a chosen nodal diameter modes in cyclic symmetric structures. In particular, a study about the minimum number and the ... [more ▼]

This paper presents a methodology enabling the appropriation and the isolation of a chosen nodal diameter modes in cyclic symmetric structures. In particular, a study about the minimum number and the optimized location of the excitation sources required in laboratory conditions to isolate a specific nodal diameter mode is conducted. This work includes a theoretical approach based on energy concepts. It is followed by the numerical and experimental validations on a stator stage of an axial compressor. The concept of modal appropriation is investigated here as the first step of the calibration procedure before engine tests. [less ▲]

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See detailPlatelet-rich plasma (PRP) to treat chronic upper patellar tendinopathies
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Bruyère, Olivier ULg et al

in British Journal of Sports Medicine (2013, July), 47(10 (e3)), 15

Background: Upper patellar tendinopathies remain often chronic and rebel to a thorough conservative treatment. Moreover, the option of a surgical treatment could be disappointing. New treatments are being ... [more ▼]

Background: Upper patellar tendinopathies remain often chronic and rebel to a thorough conservative treatment. Moreover, the option of a surgical treatment could be disappointing. New treatments are being developed. Injection of PRP is one of these. Platelets contain lot of growth factors which would have the potentiality to enhance the healing process of tendons. Even if in vitro and animal experiments have demonstrated this stimulation of tendon healing process1, clinical series are subject to controversy2. Methods: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments [VAS, clinical examination with an algometer, algofunctional scores (IKDC and VISA-P), functional assessments (isokinetic and Optojump) and imagery (ultrasounds and MRI)]were made before infiltration of PRP, and 6 weeks and 3 months after. The PRP was obtained by an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a 6-week standardised sub-maximal eccentric reeducation. Results: We observed a very significant improvement of the algofunctional status as soon as 6 weeks after the infiltration of PRP, and continued to a lesser extent up to 3 months. During functional evaluation, pain decreased as well, but without significant improvement of performances. No significant improvements in the imagery were observed. Interestingly, patients who had a VAS equal or below 1 after 3 months post-infiltration were younger (24.7 vs 32.2 y.o.). Moreover, these younger patients had a significant increase of the IKDC score (p=0.003), a significant improvement of pain during isokinetic evaluations (p<0.05), and during Optojump assessments (p=0.01). Seventy-five percent of subjects were able to return to sport, even if only half of these patients recovered the same level than before the tendinopathy. Discussion / Conclusions: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol is efficient to improve symptoms of chronic upper patellar tendinopathies, non-responsive to classical conservative treatments. However, up to now, there is no consensus on the method to prepare the PRP. Indeed, each technique could provide a very different PRP (variations in the platelet concentrations and of the amount of red and white cells). [less ▲]

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See detailDirect use of MACE EBV in the Walloon single-step Bayesian genomic evaluation system
Vandenplas, Jérémie ULg; Colinet, Frédéric ULg; Faux, Pierre ULg et al

in Journal of Dairy Science (2013, July), 96(E-Supplement),

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See detailMosquito net for abdominal wall repair: a comparison of their chemical, physical, morphological and mechanical properties to commercial meshes
Sevrin, Chantal ULg; Nsadi Fwene, Berthier; Arung, Willy et al

in Biomedica proceedings (2013, June 23)

Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor ... [more ▼]

Meshes are the actual standard in abdominal wall repair. Commercial medical meshes are however either unavailable or unaffordable too expensive for developing countries. Therefore in most resource-poor developing countries a traditional sutured repair is still commonplace although with significantly inferior results. Sterilized mosquito nets have been recently roposed by several authors to replace medical meshes in low-income countries . However in the perspective of their clinical use a better understanding of this mosquito net is obviously requested both in terms of material properties and of in vivo biocompatibility. [less ▲]

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See detailCan we use electrical resistivity tomography to measure root zone competition in fields with multiple crops?
Garré, Sarah ULg; Coteur, Ine; Wongleecharoen, Chalemchart et al

in Procedia Environmental Sciences (2013, June 20)

Contour hedgerow intercropping systems have been proposed to reduce run-off and control soil erosion on steep agricultural land. However, competition for water and nutrients between crops and associated ... [more ▼]

Contour hedgerow intercropping systems have been proposed to reduce run-off and control soil erosion on steep agricultural land. However, competition for water and nutrients between crops and associated hedgerows may reduce the overall performance of these systems. ERT measurements conducted in Thailand showed that the soils of our experimental plots were very heterogeneous both along the slope as with depth. This observation highlighted some constraints of the ERT method for soil moisture monitoring in the field. Nevertheless, the data indeed revealed contrasting water depletion patterns under monocropping and intercropping systems, which could also be related to plant parameters. [less ▲]

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See detailExcitation of nodal diameter mode-shapes of a stator ring of a turbojet engine using a limited number of excitation sources
Nyssen, Florence ULg; Golinval, Jean-Claude ULg; Viguié, Régis et al

in Proceedings of ASME Turbo Expo 2013 (2013, June 05)

When considering engine tests, the sensors used to monitor the rotor parts mainly consist in strain gauges whose information is very sensitive to the gauge positioning, especially in high stress gradient ... [more ▼]

When considering engine tests, the sensors used to monitor the rotor parts mainly consist in strain gauges whose information is very sensitive to the gauge positioning, especially in high stress gradient regions. Therefore, to reduce the risk of misinterpreting the response given by the gauges, their calibration is required. Because strain gauges are positioned so that specific modes will be monitored, their calibration must be carried out for these modes. For cyclic symmetric structures, their high spectral density imposes the use of dedicated calibration techniques. In the present paper, the concept of modal appropriation is investigated as the first step of the calibration procedure. This paper aims at presenting the development of an experimental approach enabling the isolation/appropriation of specific nodal diameter modes of a stator stage of an axial compressor using a limited number of excitation sources. A special emphasis has been brought to the minimum number and the optimized location of the excitation sources (shakers) required in laboratory conditions to appropriate specific nodal diameter modes and thereby retrieve the related modal features needed for the gauges calibration and mechanical design. [less ▲]

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See detailAmbulatory Monitoring of Teachers' Voice Use
Remacle, Angélique ULg; Morsomme, Dominique ULg; FINCK, Camille ULg

in AQL Proceedings (2013, June)

Method: Thirty-two French-speaking female teachers were monitored during one workweek (5 days) using the Ambulatory Phonation Monitor, Model 3200 (KayPENTAX). Phonation time, fundamental frequency (F0 ... [more ▼]

Method: Thirty-two French-speaking female teachers were monitored during one workweek (5 days) using the Ambulatory Phonation Monitor, Model 3200 (KayPENTAX). Phonation time, fundamental frequency (F0), sound pressure level (SPL), distance dose and cycle dose were analyzed in both professional and non-professional environments. Results: Teachers’ phonation time was statistically higher at school (20.3% ± 4.2) than not at school (10.4% ± 3.8). Concerning the fundamental frequency, both F0 average and F0 mode were statistically higher in the professional environment (F0 average = 258.7 Hz ± 20.5; F0 mode = 229.7 Hz ± 18.3) than in the non-professional environment (F0 average = 239.6 Hz ± 20.1; F0 mode = 213.3 Hz ± 20.6). The teachers spoke statistically louder at school (80.6 dB SPL ± 4.9) than not at school (74.5 dB SPL ± 5.2 dB SPL). The cycle dose and the distance dose were also statistically higher at school than in the non-professional environment. The data showed that the vocal folds vibrate 1,195,834 (± 255,696) times a day at work, plus 425,102 (±194,338) times after work. The distance traveled by the vocal folds for these teachers was 4,247 (± 1,476) meters at work, plus 1,173 (± 527) meters after work. Conclusion: Professional and non-professional voice use comparisons showed significant differences for all parameters (p<.001), indicating that vocal load was higher in the professional environment compared to the non-professional environment. [less ▲]

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See detailDrug related problems and interventions of pharmacists on prescribed medicines in Belgium
De Wulf, Isabelle; Boussery, Koen; De Vriese, Carine et al

in International Journal of Clinical Pharmacy [=IJCP] (2013, June), 35(3), 497

Aim of project/study (1) To study the frequency and nature of drug related problems (DRP) detected by community pharmacists and internship students when dispensing prescribed medicines. (2) To investigate ... [more ▼]

Aim of project/study (1) To study the frequency and nature of drug related problems (DRP) detected by community pharmacists and internship students when dispensing prescribed medicines. (2) To investigate the nature and frequency of interventions by pharmacists. (3) To study whether there is a difference between DRP detection at the moment of dispensing versus in a quiet setting. [less ▲]

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See detailExuberant inflammatory reaction after an infiltration of platelet-rich plasma (PRP)
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; LEONARD, Philippe ULg et al

in British Journal of Sports Medicine (2013, June), 47(10 (e3)), 92

Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively ... [more ▼]

Background: PRP, obtained from centrifuged autologous blood, contains a large quantity of growth factors, which may enhance the tissue healing processes. Local infiltration of PRP represents a relatively new treatment for tendinopathies. To date, no side effects have been reported after infiltration of PRP to treat tendinopathy. Case report: A 35-year-old patient had a right upper patellar tendinopathy which was resistant to all conservative treatments for more than 6 months. The patient was a type 1 diabetic (well controlled). He had an intratendinous infiltration of 6 mL of PRP (8.105 platelets/mm3, almost no red or white blood cells) after disinfection but without local anaesthetic. Immediately following the infiltration, local cryotherapy was performed for 15 minutes. NSAIDs were avoided, but class-1 or -2 pain-killers were authorised if necessary. A standardised sub-maximal eccentric rehabilitation should have been started 1 week after. However, the patient experienced local swelling with erythema, increased heating and pain which appeared just underneath the patella, but without biological inflammatory syndrome. A great Doppler signal in a thicker patellar tendon was observed by US, but there was no sign of local infectious disease demonstrated by either CT or MRI. However, the local inflammation did not decrease after a progressive 3-week treatment of local cryotherapy, local and oral NSAIDs and colchicine 1 mg. Thus, an insidious infection was suspected, even though there was neither evidence of biological inflammatory syndrome nor sign of infectious lesion on imagery examination. An antibiotic therapy (rifampicine 600 mg + minocycline 100 mg) was initiated for 3 months. Finally, a 3-phase bone scintigraphy suggested the presence of a complex regional pain syndrome type 1 treated by a classical physical therapy and concomitant class-2 pain killers. The evolution was favourable after 6 months of symptomatic treatment, and the pain decreased to a level similar to that before the infiltration of PRP. Discussion/Conclusions: This case report draws attention to potential side effects that are linked to this new therapy by infiltration of platelet rich plasma in case of tendinopathy, in particular when used in patients with type 1 diabetes. Thus, the balance between benefits and risks must be carefully evaluated before using this treatment in patients with type 1 diabetes. Reference: Platelet-rich plasma application in the management of chronic tendinopathies. Acta Orthop Belg 2013; 79: 10-15. [less ▲]

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See detailHigh-speed imaging use to predict spray retention on barley leaves
Boukhalfa, Hassina dite Hafida ULg; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

in Communications in Agricultural and Applied Biological Sciences (2013, May 21), Vol 78(2)(1-386 (2013)), 31-36

Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera ... [more ▼]

Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera coupled with a retro-LED lighting. Size and velocity of the drop were extracted by image analysis. Drop impact types were determined by the operator. Drops were produced with a flat-fan nozzle mounted on a movable ramp. Two surfactants (Break-Thru® S240 and Li700 ®) were sprayed on BBCH 12 barley leaves to highlight the effect of the reduction of surface tension. Relative volume proportions were computed within of an energy scale divided into 11 classes based on the Weber number. results are compared to the results of the chemical analysis by spectrofluerometry. [less ▲]

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See detailIs ultra-short cold ischemia the key to ischemic cholangiopathy avoidance in DCD-LT?
DETRY, Olivier ULg; DE ROOVER, Arnaud ULg; Cheham, S et al

in Acta Chirurgica Belgica (2013, May), Supplement 113(3), 6729

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See detailBiophysical characterization of the interaction of novel aromatic glycolipid surfactants with membrane models.
Sainvitu, Pauline ULg; Nasir, Mehmet Nail ULg; Nott, Katherine ULg et al

in World Academy of Science, Engineering and Technology (2013, May), 77

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic ... [more ▼]

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as a function of their aromatic group. Their interaction with membranes was studied with monolayer models and was predicted by a computational approach. The relationships between these data and their cytotoxicity and antioxidant properties evaluated on cell cultures are discussed. [less ▲]

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See detailEvaluation of the performance of the new automated IDS-ISYS 1,25-dihydroxy vitamin D assay
GADISSEUR, Romy ULg; LUKAS, Pierre ULg; CARLISI, Ignazia ULg et al

in Biochimica Clinica (2013, May), 37(SS), 498

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See detailEvaluation of the third-generation to second-generation PTH ratio on the Liaison XL (DIASORIN) as a marker for parathyroïd carcinoma
Schleck, Marie-Louise; LAURENT, Terry ULg; GADISSEUR, Romy ULg et al

in Biochimica Clinica (2013, May), 37(SS), 161

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See detailTherapeutic Strategy and Patient Outcome during the First 2 Years of Pediatric Crohn’s Disease
Veereman, G; Mahachie John, Jestinah ULg; De Greef, E et al

in Journal of Pediatric Gastroenterology and Nutrition (2013, May)

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See detailImpact of strenuous exercise on the release of cardiac biomarkers
LE GOFF, Caroline ULg; MELON, Pierre ULg; Kaux, Jean-François ULg et al

in Biochimica Clinica (2013, May), 37(SS), 545

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured ... [more ▼]

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured by new commercially available high-sensitive methods in subjects un-dergoing the Maasmarathon. Our aim was to compare cTnT and NT-proBNP levels before and after the stress tests, in sportive subjects. Methods: Twenty eight subjects (26 ♂, 42.5±11yo) underwent a race of 42.195 kilometers be-tween Visé (Belgium) and Maastricht (The Netherlands). We drowned blood sample before (T0), just after (T1) and three hours after the race (T3). In all patients, cTnT concentrations were measured by high sensitive methods (hsTnT, Roche Diagnostics) on heparin plasma. The NT-proBNP was also determined with the kit Roche on heparin plasma. The protocol was approved by the ethics committee of the University of Liège (Belgium). All subjects gave their informed consent. All statistical analyses were performed using Medcalc version 8.1 for Windows. p-value <0.01 was regarded as statistically significant. Results: There was a significant difference between hsTnT concentrations at T0 and T1 (p<0.0001), and between T0 and T3 (p<0.001) for NT-proBNP, but not between T1 and T3. This observation appeared only after a strenuous exercise but today this type of exercise is not reproduce easier in a laboratory of sport. Moreover, at this moment, nobody knows if these observations would have cardiac consequences at long terms. Conclusions: Measurement of cardiac troponins by high sensitive methods allows detecting significant release of biomarkers from the heart during exercise. The value of NT-proBNP are also significant but less than TnThs. We think that the TnThs could be an interesting tool in the future to help sport medicine to detect risk of developing a cardiac problem in the future or a sudden death. [less ▲]

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See detailDetermination of urinary metanephrine, normetanephrine and methoxymetanephrine by liquid chromatography-electrospray tandem mass spectrometry.
LE GOFF, Caroline ULg; PEETERS, Stéphanie ULg; NETCHACOVITCH, Matthieu ULg et al

in Biochimica Clinica (2013, May), 37(SS), 316

Background: The aim of this work was to develop and validate a method for the determination of metanephrine (M), normetanephrine (NM) and methoxymetanephrine (METHO) in urine by liquid chromatography ... [more ▼]

Background: The aim of this work was to develop and validate a method for the determination of metanephrine (M), normetanephrine (NM) and methoxymetanephrine (METHO) in urine by liquid chromatography-tandem mass spectrometry (LCMS-MS) on the Triple Quad TQ 5500 from AB SCIEX. In fact, the determination of M and NM concentrations is used in clinical diagnosis of pheochromocytoma, a rare but potentially fatal tumor arising primarily from the chromaffin cells of the adrenal medulla. Methods: The samples were made of 24 hours acidified urines after centrifugation. Sample preparation was performed by hydrolysing and purifying by extraction column. After that, labeled M, NM and METHO were added as internal standard. Samples were analysed by liquid chromatography-electrospray tandem mass spectrometry. We determined the repeatability, reproducibility, accuracy profile and recovery on pooling urines samples from 9 volunteers analysed in triple run. Results: The results of the precision evaluation are shown in table. The repeatability did not exceed 8.4 % for M, 6.8% for NM and 10.8% for METHO. The concentration range was 71-781 µg/24h, 71-853 µg/24h and 20-854µg/24h for the M, NM and METHO respectively. The total precision did not exceed 12.5%, 11.8% and 8.8% for M, NM and METHO. The limit of quantification (LOQ) were 33.77µg/24h, 14.49µg/24H and 19.81 µg/24H for M, NM and METHO respectively. The accuracy varied from 99.69 to 100.2% for a range of 71 to 781 µg/24h, from 93.32 to 100.2% for a range of 71-853 µg/24h and from 99.85 to 100.6% for the range 20-854µg/24h for M, NM and METHO respectively. The recovery were 99.96% (95% CI for the mean: 96.5-103.4), 99.75% (96.5-102.9) and 100.08 (95.97-104.2) for the M, NM and METHO respectively. Conclusions: We have successfully developed and validated an LCMS-MS method to determine urinary M, NM and METHO on the TQ 5500 from AB SCIEX. It represents a convincing alternative to the HPLC method for a faster and reliable measurement of urinary M, NM and METHO. [less ▲]

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See detailHeavy/light chain (HLC) and free light chain (FLC) analysis allow sensitive monitoring of multiple myeloma patients and AID detection of clonal changes
Dierge, Laurine; LUTTERI, Laurence ULg; Chauvet, D et al

in Biochimica Clinica (2013, May), 37(SS), 618

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See detailRace Cycling: biological evolution
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; MELON, Pierre ULg et al

in Biochimica Clinica (2013, May), 37(SS), 544

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 179.6 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cell (RBC), haemoglobin (HgB), creatinin (Cr), highly sensitive troponin T (hsTnT), myoglobin (MYO) and NT-proBNP. All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results: RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002). Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001). These changes might be related to renal flow depletion during exercice. MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014). The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001) .The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP. These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusion: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]

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See detailComparison of the Heart-type fatty acid-binding protein (H-FABP) with the high sensitive cardiac troponin T in healthy runners
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; BREVERS, Eric ULg et al

in Biochimica Clinica (2013, May), 37(SS), 544

Background: Heart-type fatty acid-binding protein (H-FABP) is a low molecular weight protein involved in the intracellular uptake and buffering of long chain fatty in the myocardium. It is an early marker ... [more ▼]

Background: Heart-type fatty acid-binding protein (H-FABP) is a low molecular weight protein involved in the intracellular uptake and buffering of long chain fatty in the myocardium. It is an early marker for acute coronary syndrome. Troponin T (TnT) is a component of the contractile apparatus of the striated musculature. Cardiac TnT is a cardio-specific, highly sensitive marker for myocardial damage. The aim of our study was to compare the results obtained with the H-FABP and the highly sensitive cardiac troponins (hsTnT) and to test their cardiospecificity in healthy runners. Methods: Twenty three runners (marathon) were enrolled. We drowned samples at three times: just before (T0), just after (T1), and three hours after the end of the race (T3). H-FABP was determined with a Randox immunoturbidimetric assay and hs-TnT with a Roche electrochemiluminescence immunoassay, both on Cobas 6000. A linear regression was calculated to observe if there is any correlation between the two biomarkers. Values above the 95th percentile for H-FABP (2.5ng/mL) and the 99th percentile for hsTnT (14ng/L) were considered as positive. Results: At T0, none of the subjects were positive for hsTnT but 35% were positive for H-FABP; at T1, 83% for hsTnT and 100% for H-FABP; at T3, 83% for hsTnT and 96% for H-FABP. At T0, the regression equation was H-FABP T0 = 3.9454 – 0.1001 x hsTnT T0; at T1: H-FABP T1 = 51.838 – 1.7026 x hsTnT T1; at T3: H-FABP T3 = 47.977 – 1.6193 x hsTnT T3. No correlation was observed between the two biomarkers at the different time. Conclusions: We observed a significant increase of H-FABP and hsTnT in runners. These markers are independent to each other. These values could biologically correspond to a heart ischemia. However, we suggested that exercise-induced cardiac hsTnT and H-FABP release is not a marker of exercise-induced pathology but likely a physiologic response to effort or an exercise-induced cardiac remodelling. [less ▲]

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See detailBiophysical characterization of the interaction of novel aromatic glycolipid surfactants with membrane models.
Sainvitu, Pauline ULg; Nasir, Mehmet Nail ULg; Nott, Katherine ULg et al

in World Academy of Science, Engineering and Technology (2013, May), 77

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic ... [more ▼]

Aromatic glycolipids are of both medical and pharmaceutical interest. Antimicrobial, antiviral and anti-inflammatory activities have been reported (Otto, 2000, Journal of Molecular Catalysis B: Enzymatic). Moreover, they are expected to have interesting antioxidant properties when they contain phenolic groups. The alkyl chain should enhance their ability to penetrate into the cellular membrane (Nicolosi, 2002, Journal of Molecular Catalysis B: Enzymatic). The presence of a sugar unit could also be useful to target specific cells. In this study, novel aromatic glycolipids were synthesized as useful models for studying the structure–activity relationship, in particular as a function of their aromatic group. Their interaction with membranes was studied with monolayer models and was predicted by a computational approach. The relationships between these data and their cytotoxicity and antioxidant properties evaluated on cell cultures are discussed. [less ▲]

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See detailComparison between Perkin-Elmer and Chromsytem Vitamin D kit on TQ 5500 from AB SCIEX
LE GOFF, Caroline ULg; PEETERS, Stéphanie ULg; CRINE, Yannick ULg et al

in Biochimica Clinica (2013, May), 37(SS), 471

Background: Twenty-five hydroxy-vitamin D (25(OH) D) determination is now routinely prescribed in the Laboratory. Recently, different new methods have been available for this determination. Among them ... [more ▼]

Background: Twenty-five hydroxy-vitamin D (25(OH) D) determination is now routinely prescribed in the Laboratory. Recently, different new methods have been available for this determination. Among them, LCMS/MS methods have emerged in some laboratories. However these methods are generally “home-brewed” and an important variability between them can be seen on different external quality controls, mainly due to a lack of standardization. Recently, Perkin-Elmer (PE) (Turku, Finland) and Chromsystem (CS) (Grafelfing, Germany) launched a standardised method for 25(OH )D determination on LCMS/MS. The aim of our study was to compare these methods on the AB SCIEX TQ5500 (Framingham, Massachusetts, USA) LCMS/MS to measure 25(OH) D3. Methods All the samples were treated according to our preanalyitical procedure: after sampling, they were spun at +4°c at 3500G, aliquoted and kept frozen at -20°c until determination. A method comparison was assessed with CS and PE for the measurement of the 25(OH)D3. We selected 110 remnant samples with 25(OH)D3 levels ranging from 1.6 to 136.7 ng/ml with the PE method to cover the range of usually values Slope and intercept were calculated using Passing and Bablock linear regression and we compared the methods with the Bland and Altman plots. Results For CS, the method is linear up to 250 µg/L, the LOQ is 3.6 µg/L, the intra-assay CV is < 5% and the inter-assay is < 7%. For PE, the method is linear up to 314 µg/L, the LOQ is 3.4 µg/L, the intra-assay CV is < 7.8% and the inter-assay is < 8.5%. On the whole range of measure (n=110), the regression equation is PE = 0.8521+0.9226 (CS) (95%CI of the intercept: (-0.0048;1.37) and 95% CI of the slope (0.89;0.95). The Bland and Altman plot does not show any bias between the two methods (mean difference CS-PE= -2.5 ng/ml) and the standard deviation of the mean is 3,98 ng/ml Conclusion: The performances of these methods are comparable on our new TQ 5500 from AB SCIEX. For now, there is no consensus on a “reference” method for vitamin D quantification. We notice only that the values obtained by CS are systematically a little bit lower than PE’s values, especially for results below 20 ng/ml. However, we have no clear explanation for such behaviour. [less ▲]

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See detailMDRD VERSUS CKD-EPI EQUATIONS TO ESTIMATE GLOMERULAR FILTRATION RATE IN OBESE PATIENTS
BOUQUEGNEAU, Antoine ULg; CAVALIER, Etienne ULg; Krzesinski, Jean-Marie ULg et al

in Nephrology Dialysis Transplantation (2013, April 18), 28(supplément 1), 117-139

Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially ... [more ▼]

Introduction and Aims: Obesity is recognized as a risk factor both for the development and progression of chronic kidney disease (CKD). Estimating glomerular filtration rate (GFR) is thus especially important to follow these patients. We have tested the performances of two creatinine-based equations, namely the MDRD and CKD-EPI equations, in an obese population. Conclusions :Both in the global and subgroup analyses, the CKD-EPI equation did not outperform the MDRD study equation. The performances of both equations were worse in CKD patients. These two conclusions were still valid if indexed GFR was considered. [less ▲]

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See detailImmune Reconstitution After Alternative Hematopoietic Stem Cell Transplantation: Comparison of Unrelated Cord Blood and Mismatched Unrelated Donor Hematopoietic Stem Cell Transplantation
Servais, Sophie ULg; Lenglinne, Etienne; Porcher, Raphael et al

in Bone Marrow Transplantation (2013, April 08), 48(S2), 132

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See detailOARSI recommended performance-based tests to assess physical function in people with established hip and knee osteoarthritis
Dobson, F.; Hinman, R.S.; Roos, E.M. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

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See detailSuperior Renal Function Sustained for 24 Months through Early Everolimus-Facilitated Reduction of Tacrolimus Versus Standard Tacrolimus in De Novo Liver Transplant Recipients: Results of a Randomized Trial.
De Simone, Pierre; DETRY, Olivier ULg; Kintmalm, G et al

in American Journal of Transplantation (2013, April), 13(S5), 169450

mTOR inhibitors have the potential to reduce calcineurin inhibitor nephrotoxicity by minimizing or eliminating the need for their use. The 12 month (M) results of H2304 (NCT00622869) study demonstrated ... [more ▼]

mTOR inhibitors have the potential to reduce calcineurin inhibitor nephrotoxicity by minimizing or eliminating the need for their use. The 12 month (M) results of H2304 (NCT00622869) study demonstrated superior renal function with everolimus (EVR) plus reduced tacrolimus (rTAC) vs. standard TAC (TAC-C) in de novo liver transplant recipients (LTxR). Presented here are 24M renal function results. For this 24M, multicenter, open-label study 719 de novo LTxR were randomized (1:1:1) after a 30-day (±5 days) run-in period with TAC (±mycophenolate mofetil), to receive either EVR (C0 3-8 ng/mL) with rTAC (C0 3-5 ng/mL; EVR+rTAC, N=245) or EVR (C0 6-10 ng/mL) with TAC withdrawal (TAC-WD; N=231) at M4 or TAC-C (C0 6-10 ng/mL; TAC-C, N=243); all patients received corticosteroids. Enrollment in TAC-WD arm was stopped early due to higher rejection rates. Main endpoints at M24 included composite ef􏰀cacy failure rate of treated biopsy proven acute rejection, graft loss or death, and evolution of renal function from randomization (RND) to M24 measured as eGFR by MDRD4. At M24, composite ef􏰀cacy failure rate in EVR+rTAC arm was comparable to TAC-C (10.3% vs. 12.5%, p=0.452). Evolution of renal function from RND to M24 was superior for EVR+rTAC vs. TAC-C with an adjusted mean difference in eGFR change of 6.66 mL/min/1.73m2 (p=0.0018; ITT population). Signi􏰀cantly higher eGFR with EVR+rTAC was achieved at M2 post-LTx and was maintained until M24. On-treatment data showed a decrease in mean eGFR from RND to M24 of 6.6 mL/min/1.73m2 with EVR+rTAC vs. 13 mL/min/1.73m2 with TAC-C and 2.5 mL/ min/1.73m2 gain with TAC-WD. Urinary protein:creatinine ratio (mg/g) at M24 was higher with EVR+rTAC vs. TAC-C (Mean±SD: 194±280 vs. 159±284, p=0.006). Early introduction of EVR at 1M post-LTx with rTAC showed superior renal function sustained for 24M compared to TAC-C, without compromising ef􏰀cacy in de novo LTxR. [less ▲]

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See detailOrthanc - A lightweight, RESTful DICOM server for healthcare and medical research
JODOGNE, Sébastien ULg; Bernard, Claire ULg; DEVILLERS, Magali ULg et al

in Proceedings, IEEE International Symposium on Biomedical Imaging: from Nano to Macro (2013, April)

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See detailStrontium ranelate improves osteoarthritis symptoms compared to placebo in patients with knee OA: The SEKOIA study
Bruyère, Olivier ULg; Richette, P; Bellamy, N et al

in Osteoporosis International (2013, April), 24(Suppl.1), 49-51

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See detailChanges in the structure and the symptoms of the osteoarthritis knee and prediction of future knee replacement over an 8-year follow-up period
Bruyère, Olivier ULg; Cooper, Cyrus; Pavelka, Karel et al

in Osteoporosis International (2013, April), 24(Suppl.1), 209

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See detailEffects of chondroitin sulfate on the gene expression profile in the inflamed synovial membrane
Lambert, Cécile ULg; Dubuc, J-E; Montell, E. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a ... [more ▼]

Purpose: The aim of the present work was to identify the differentially expressed genes between the inflammatory (I) and normal/reactive (N/R) synovial areas using a unique ex vivo culture model. In a second step, we investigated the genetic modulatory effects of chondroitin sulfate (CS) in this model. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days in the absence or in the presence of highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain). Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I conditions, N/R and N/R-CS conditions and I and I-CS conditions was based on paired t-test where N/R and I, N/R and N/R-CS and I and I-CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Results: From among 47000 probes, 18253 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 465 differentially expressed genes between N/R and I areas were identified. Many inflammatory mediators appear differentially expressed. The interferon alpha-inductible protein 6 (IFI6) was the most up-regulated. We also identified the hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), the cathepsin K (CTSK), the chemokine (C-X-C motif) ligand 1 (CXCL1) and the EBV-induced G-protein coupled receptor 2 (EBI2). The differential expression of intermediates involved in angiogenesis pathway was also revealed between N/R and I areas. Among them, R-spondin-3 (RSPO3), the secreted phopshoprotein 1 (SPP1) and aquaporin 9 (AQP9) were up-regulated whereas ADAMTS1 was down-regulated. Finally, in the Wnt signaling, RSPO3 was up-regulated unlike dickkopf homolog 3 (DKK3) which was in turn down-regulated. We next performed a class comparison test between N/R and N/R-CS in one hand and between I and I-CS the other hand. 489 genes were identified as differentially expressed genes between N/R and N/R-CS conditions while 219 genes were identified between I and I-CS conditions. In this latter, our attention was focused on the down-regulated genes. Among them, we identified a number implicated in angiogenesis and cell migration pathways. Thus, the endothelial cell-specific molecule-1 (ESM1), the Transmembrane-4-L-six-family-1 (TM4SF1), the 5’-Ectonucleotidase (NT5E) and the growth arrest-specific gene 6 (GAS6) were down-regulated by CS. Conclusions: Our work demonstrates the differential gene expression profile between paired non inflammatory and normal/reactive areas of synovial membrane as well as the modulatory effects of CS on gene expression in the inflammatory areas, especially regarding genes involved in both angiogenesis and cell migration. [less ▲]

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See detailInvestigation of potential new targets for the diagnosis and/or the treatment of osteoarthritis
Lambert, Cécile ULg; Dubuc, J.-E.; Montell, E. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory ... [more ▼]

Purpose: Synovial inflammation plays a key role in the pathophysiology process of osteoarthritis (OA). We have previously compared the gene expression pattern of synovial cells isolated from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same OA patient. We identified a large number of mediators belonging to key pathways involved in OA pathogenesis. The aim of this study was to validate different potential new targets for the diagnosis and/or the treatment OA. Methods: Synovial cells (SC) were isolated from synovial specimens obtained from OA patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria. The biopsies from N/R and I areas were cultured separately for a period of 7 days. Microarray gene expression profiling between N/R and I areas was performed. The biological relevance of up- and down-regulated genes was analyzed with Ingenuity Pathways Analysis. Western blot and immunohistochemistry confirmed the identified genes most differentially expressed in the key pathways. The production of the triggering receptor expressed on myeloid cells-1 (TREM1), the alarmin S100 calcium binding protein A9 (S100A9), the wingless-type MMTV integration site family, member 5A (Wnt-5A) and the stanniocalcin 1 (STC1) were evaluated by Western blot. S100A9, hyaluronan synthase-1 (HAS1) and STC1 expression and localization were investigated by immunohistochemistry. Results: 896 genes differentially expressed in N/R and I areas were identified. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling and angiogenesis. In the inflammatory gene pattern, TREM1 and S100A9 were strongly upregulated. We validated the production of these proteins in OA synovial biopsies by Western blot. TREM1 and S100A9 were increased in I compared to N/R synovial cells culture. S100A9 was observed in the perivascular area and in sublining cells in I synovial biopsies, but not in N/R biopsies. An increased staining was also observed in the intima lining layer of I when compared to N/R biopsies. The most upregulated anabolism enzyme in I synovial biopsies was HAS1. Using immunohistochemistry, we observed in I areas an increase of the HAS1-positive cells mainly in the intima lining. We also studied the protein production of Wnt-5A, the most upregulated intermediate of Wnt signaling pathway. The protein level was increased in I compared to N/R areas. Finally, in the angiogenesis pathway, one the most u-regulated gene was STC1. A significant increase of STC1 production was observed in I areas compared to N/R areas by Western blot. This result was also supported by the immunohistochemical analysis. In I area, the staining for STC1 was more intense in perivascular and sublining cells. Conclusions: Synovial membrane inflammation is a key target for OA treatments. In this work, we have identified proteins involved in the synovitis pathways like angiogenesis, cells infiltration and matrix remodeling. These proteins could be targeted by drugs and used as companion biomarkers for evaluating their efficacy. Although qualitative, our results could also yield to the identification of markers of the disease. This investigation has to be further pursued. [less ▲]

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See detailRole of nutraceuticals in the symptomatic and structural management of osteoarthritis
Bruyère, Olivier ULg

in Osteoporosis International (2013, April), 24(Suppl.1), 82-83

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See detailHealth economics in osteoarthritis
Hiligsmann, Mickaël ULg; Cooper, Cyrus; Arden, Nigel et al

in Osteoporosis International (2013, April), 24(Suppl.1), 79-80

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See detailReproducibility of joint space width assessment when external calibration on the radiograph is missing
DEROISY, Rita ULg; Reginster, Jean-Yves ULg; Bruyère, Olivier ULg

in Osteoporosis International (2013, April), 24(Suppl.1), 379

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See detailValidity of the French hip and knee replacement expectations surveys
NEUPREZ, Audrey ULg; François, Garance ULg; Delcour, JP et al

in Osteoporosis International (2013, April), 24(Suppl.1), 374

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See detailQuality of life in sarcopenia and frailty
Rizzoli, René; Reginster, Jean-Yves ULg; Arnal, Jean-François et al

in Osteoporosis International (2013, April), 24(Suppl.1), 76-77

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See detailWhat is the predictive value of MRI for the occurrence of hard clinical endpoints in knee osteoarthritis?
Pelletier, Jean-Pierre; Peterfy, Charles; Brandi, Maria Luisa et al

in Osteoporosis International (2013, April), 24(Suppl.1), 84-85

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See detailCost-effectiveness of vitamin D and calcium supplementation in the treatment of postmenopausal women
Hiligsmann, Mickaël ULg; Ben Sedrine, Wafa ULg; Rabenda, Véronique ULg et al

in Osteoporosis International (2013, April), 24(Suppl.1), 198

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See detailEvaluation of the impact of a 6-month training by whole body vibration on the risk of falls among nursing home residents
Beaudart, Charlotte ULg; Buckinx, Fanny ULg; Demonceau, Marie ULg et al

in Osteoporosis International (2013, April), 24(Suppl.1), 246-247

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See detailThe intra-articular injection of a new chitosan biomaterial prevents the progression of osteoarthritis in ACLT rabbit model
Oprenyeszk, Frédéric ULg; Chausson, Mickael; Maquet, Véronique et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013), 69

Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on ... [more ▼]

Purpose To evaluate the effects of a single intra-articular injection of a new biomaterial consisting in a mix of alginate-chitosan (AC) beads and a viscous thermogelling chitosan-based (H) hydrogel on cartilage lesion in osteoarthritis (OA) rabbit model. These effects were compared to those obtained with the intra-articular injection of either chitosan-based (H) hydrogel without the AC bead or saline solution. Methods OA was surgically induced by the transection of the anterior cruciate ligament (ACLT) in HYLA albino rabbits. One week after surgery, animals were randomly divided into 3 groups: group I (n=7): mix of AC beads and H hydrogel; group II (n=7): H hydrogel alone; group III (n=7): saline solution (control). The treatments (900 µl) were injected intra-articularly. X-rays from the right knee were performed before surgery, at the time of injection and at sacrifice. The standard radiographs were acquired in extension and scored by the Kellgren and Lawrence (K&L) scale. After 6 weeks, animals were euthanized and the right joint was dissected. The macroscopic evaluation of cartilage from femoral condyles and tibial plateaus stained with India ink was done. Histological sections stained with Safranine-O/fast green from bearing areas of each compartment were evaluated according to the OARSI histological score. Briefly, the evaluation considered: staining of the cartilage matrix (0-6), cartilage structure (0-11), chondrocyte density (0-4) and cluster formation (0-3), where 0 represented a normal situation and 24 points the maximum severity score. Blood samples were collected the day of injection and prior the sacrifice. Prostaglandin E2 (PGE2) and C-reactive protein (CRP) were measured in serum using immunoassays. Results The X-rays analysis showed a significant decrease (p <0.05) of the K&L score in group I (AC beads and H hydrogel; 1.5 ± 0.2) compared with group II (H hydrogel; 2.2 ± 0.5) and group III (saline solution; 3.0 ± 0.4). The size and the severity of the macroscopic OA cartilage lesion tended to decrease in group I compared to the other groups. The histological global score that refers to all compartments of the knee joint was significantly decreased in group I (11.0 ± 0.7) compared to group II (14.4 ± 0.6, p <0.01) and group III (14.8 ± 0.6, p <0.001). No significant variation of PGE2 and CRP serum levels were observed in each after 6 weeks follow-up whatever the treatment injected. Conclusions This study showed that a biphasic hydrogel composed by AC beads and H hydrogel prevented OA in rabbit with ACL transection. This effect was not observed with the hydrogel alone, suggesting that AC beads play a role in joint protection. The preventive effect was observed in all joint compartments indicating a global protective effect of this new viscosupplementation. [less ▲]

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See detailScalar triplet leptogenesis without right-handed neutrino decoupling
Aristizabal Sierra, Diego ULg

in Nuclear Physics B Proceedings Supplement (2013, April), 237-238

We discuss leptogenesis in the context of type-II seesaw in the case in which in addition to the scalar electroweak triplet decays the lepton asymmetry is also induced by right-handed neutrino decays ... [more ▼]

We discuss leptogenesis in the context of type-II seesaw in the case in which in addition to the scalar electroweak triplet decays the lepton asymmetry is also induced by right-handed neutrino decays (mild hierarchical scenarios). We show that within this setup, depending on the relative sizes of the relevant parameters, one can identify three classes of generic models, each one with its own consequences for leptogenesis. [less ▲]

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See detailEffects of chondroitin sulfate on the gene expression profile in IL-1β stimulated synovial fibroblast cells cultures
Lambert, Cécile ULg; Dubuc, Jean-Emile; Montell, E. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control ... [more ▼]

Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control and IL-1β stimulated synovial fibroblast cells cultures. In a second step, we investigated the effects of CS on this gene expression profile. Methods: OA synovial specimens were obtained from 12 patients undergoing knee replacement. At the surgery time, the synovial membrane was dissected. Synovial fibroblast cells (SFC) were enzymatically isolated and used after four passages (P4). SFC were pre-treated 1 hour with highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain) before treatment with IL-1β (1 ng/ml) for 24 hours. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class comparison test between control (Ctl) and interleukin (IL)-1β conditions, Ctl and Ctl/CS and IL-1β and IL-1β/CS conditions was based on paired t-test where Ctl and IL-1β, Ctl and Ctl/CS and IL-1β and IL-1β/CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Probes with a p-value below 0.001 were chosen and classified as up- or down-regulated ones. Results: 3308 genes were identified as differentially expressed genes between Ctl and IL-1β conditions. We observed a differential profile of expression of major pathways involved in OA pathogenesis. The key identified pathways were related to inflammation, complement cascade, angiogenesis, cartilage catabolism and anabolism and Wnt signaling. In the inflammatory network, the most upregulated cytokines were IL-8 and IL-6 with a fold change of 156.25 and 58.8 respectively. We also identified several chemokines, enzymes and metallothioneins (MTs). Complement factor B (CFB) and complement component 3 (C3) are two factors upregulated in the inflammatory complement cascade. We also identified some genes implicated in the angiogenesis pathway. The most upregulated was Stanniocalcin 1 (STC1) with a fold change of 9.09. The differential expression of intermediates involved in both cartilage anabolism and catabolism was revealed by the IL-1β stimulation, showing an imbalance in favour of catabolism. MMP-3 was largely upregulated (fold change of 62.5). Wnt 5A and low density lipoprotein receptor-related protein (LRP8) were significantly upregulated while frizzled homolog 2 (FZD2) and dickkopf homolog 3 (DKK3) were downregulated in the Wnt signaling pathway. We next performed a class comparison test between Ctl and Ctl/CS in one hand and IL-1β and IL-1β/CS on the other hand. 660 genes were identified as differentially expressed between Ctl and Ctl/CS conditions while 241 genes were identified between IL-1β and IL-1β/CS. Among them, our attention was focused on two genes upregulated in the presence of CS: lysyl oxidase-like 4 (LOXL4) and claudin 11 (CDLN11), two genes that negatively regulate cell invasion. Conclusions: We here evidenced in synovial fibroblast cells the modulation of gene expression following IL-1β stimulation. We also demonstrated the modulatory effects of CS on gene expression and isolated several CS-modulated genes of interest such as LOXL4 and CDLN11, which could constitute new mechanisms of action of the molecule and contribute to explain the symptomatic efficacy of CS in the treatment of OA. [less ▲]

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