Accompagnement des aidants des patients Alzheimer comme approche non-pharmacologique essentielle!

in Revue Francophone de Gériatrie et de Gérontologie (2012, October), XIX(188), 326-327

Investigation on High Efficiency Volume Bragg gratings performances for spectrometry in space environment

in ICSO Filter & Grating (2012, October)

Cluster headache Award 2012: Central modulation in cluster headache patients treated with occipital nerve stimulation

in Journal of Headache & Pain (2012, September 16)

Aging of the newly diagnosed acromegalic patients : data frome the LAS (Liege Acromegaly Survey)

in Journal für Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3), 33

Age, GH and tumor size : the triangular relation in acromegaly. Data from the LAS (Liege Acromegaly Survey)

in Journal für Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3), 43

Erythropoiesis in acromegaly : effect of GH or IGF-1 ? Data from the LAS (Liege Acromegaly Survey)

in Journal für Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3), 45

A salt tracer test monitored with surface ERT to detect preferential flow and transport paths in fractured/karstified limestones

in Berichte der Geologischen Bundesanstalt (2012, September), 93

Aires et agrandissements. Maths & Manips pour la transition primaire-secondaire

in Losanges (2012, September), 18

GH or IGF-1 : which one is raising blood glucose ? Hints from the LAS (Liege Acromegaly Survey)

in Journal of Klinische Endokrinologie und Stoffwechsel (2012, September), 5(3),

Impact of extracorporeal shock wave therapy in the treatment of chronic lateral epicondylitis

in Regional Anesthesia & Pain Medicine (2012, September), 37(5 - Suppl 1), 263

Background and aim: radial shock wave therapy (RSWT) is a relatively new way to treat chronic tendinopathies, such as lateral epicondylitis. However, very few studies have been realized on this subject ... [more ▼]

Background and aim: radial shock wave therapy (RSWT) is a relatively new way to treat chronic tendinopathies, such as lateral epicondylitis. However, very few studies have been realized on this subject, and the results are very divergent. We aimed to observe the impact of this technique on chronic lateral elbow pain. Method: fifteen subjects who had a lateral epicondylitis for at least 3 months were included in the study. Two groups were formed: experimental (10 subjects) who received 6 sessions of physiotherapy and RSWT, and the control group (5 subjects) who received exclusively physiotherapy. Physiotherapy sessions were composed of massage, stretching, diacutaneous fibrolysis, and a muscular eccentric program of the wrist extensor muscles. We evaluated the subjects before the first session, and after 6 weeks of treatment with a pain visual analog scale (VAS), the painless wrist flexion amplitude, ant the “Patient-Rated Tennis Elbow Evaluation” (PRTEE) questionnaire. Results: the difference between initial and final evaluations was significant (regarding to the wilcoxon test) for all of the parameters studied (p=0,028 for the VAS, p=0,005 for the wrist flexion amplitude, and p=0,005 for the PRTEE) in the experimental group. It wasn’t significant in the control group (VAS p=0,144128, wrist flexion amplitude p=0,079617, and PRTEE p=0,067890). The comparison between the two groups was not significant, neither in the beginning, nor in the end of the treatment (regarding to the Umann and Whitney test) Conclusions: RSWT associated to physiotherapy is a more effective treatment for lateral epicondylitis than physiotherapy alone. [less ▲]

Change in naming abilities between the ages of 50 and 90: The importance of analyzing naming latency

in Stem-, spraak- en taalpathologie (2012, September), 17(2), 126-128

This study tests the controversial hypothesis that word naming difficulties may arise in individuals as young as their 50s. Participants of 25-35, 50-59, 60-69 and above 70 years of age were given a ... [more ▼]

This study tests the controversial hypothesis that word naming difficulties may arise in individuals as young as their 50s. Participants of 25-35, 50-59, 60-69 and above 70 years of age were given a picture naming task. To uncover subtle naming difficulties, correct naming latencies were analyzed, in addition to accuracy. Moreover, in order to control whether the expected slower naming latencies could be due to a general slowing affecting all cognitive tasks, participants were also given an odd/even judgment task to assess cognitive processing speed. In participants in their 50s, we found subtle naming difficulties revealed by longer naming latencies, unaccompanied by any decrease in naming accuracy. The age-related naming disadvantage increased with age with the onset of naming errors. Thus, in adults in their 60s and their 70s, the results showed both a decrease in accuracy and an increase in correct naming latencies. Moreover, the increase in naming latencies remained significant even after controlling for odd/even judgment latencies, suggesting a degradation specific to the picture naming task. We assumed that these slower latencies may result from a language-specific impairment. [less ▲]

Effect of artificial insemination site on post-mating endometritis in mares

in Reproduction in Domestic Animals (2012, August), 47(suppl 5), 103

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were ... [more ▼]

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were inseminated with a same batch of frozen semen for 3 consecutive cycles. Mares were inseminated with the following procedures in a random order: (1) Deep uterine insemination with 4ml of semen; (2) Horn bifurcation with 4ml of semen; (3) Horn bifurcation with 4ml of semen and 6ml of extender. During each cycle, Cotton (C) and Brush (B) swabs were collected at 4 different moments: mid- dioestrus, mares with a 35mm follicle, 24h and 6 days after AI. Swabs were smeared on slides, fixed and stained (Diff-Quick®) before examination under light microscopy. Proportions of inflammatory and epithelial cells were determined and differences were studied with kruskal-wallis test for non-parametric data. Distensions of uterine lumen due to intraluminal fluid observed during ultrasound exams were measured and recorded. Quality of slides was better (p=0.0006) with B swabs than C swabs with 97% versus 65% of slides readable. B swabs were associated with higher percent of endometrial cells retrieved (p=0.0323), making them a better tool for endometritis diagnosis, which is consistent with our previous reports. Volume of intraluminal fluid and percent of inflammatory cells, both on B and C swabs, were not influenced by AI location regardless the timing of sampling. Small volume deep uterine AI did not significantly affect inflammatory response by the endometrium in our experiment. [less ▲]

Environmental enrichment can accentuate condtioned reward induced by representative cocaine doses in mice

in Journal of Psychopharmacology (2012, August), 26(8), 70

Squamous metaplasia of the prostate and diffuse alopecia in a 13-year-old castrated dog due to chronic ingestion of exogenous estradiol

in Reproduction in Domestic Animals (2012, August), 47(S4), 561

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to ... [more ▼]

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to exclude or confirm Cushing disease. The dog had previously been treated with trilostane for 4 months without having any positive effects. Earlier blood analysis showed no significantly abnormalities and ACTH stimulation test was negative. Having bilateral cryptorchidism, the dog was castrated at young age. Clinical findings and treatment. Abdominal ultrasound revealed a slightly enlarged right adrenal gland (diameter of 1 cm at the cranial part). The prostate was enlarged and heterogeneous with multiple anechogenic cavities, indicating hormonal stimulation. Craniad the prostate and ventrally of the bladder, a hypoechogenic, oval structure (3 cm by 1.7 cm) could be seen, and was suspected to be a remaining cryptorchide testicle. Fine needle aspiration however showed that the structure contained a thick purulent liquid, with large amounts of polymorhonuclear white blood cells. Blood analysis revealed a leukocytosis (29370/mm3) with neutrophilia (23202/mm3). Low-dose dexamethasone test definitely excluded Cushing disease (basal cortisol levels 2,17 µg/dl; cortisol at 4 and 8 hours < 1 µg/dl). A prostatic wash was performed and revealed a severe prostatitis and presence of keratinized prostatic cells, indicating a squamous metaplasia of the prostate. Measurement of seric LH levels (LH Witness®, Synbiotics) showed an LH concentration lower than 1 ng/ml, indicating a hormonal negative feedback on the hypothalamo-hypophyso-gonadal axis. After a more thorough anamnesis, it became clear that the dog was licking and ingesting a transdermal estradiol containing cream (Estrogel®) from his owner, since two years, causing his symptoms. The dog was put on antibiotic treatment for the prostatitis (enrofloxacine 10 mg/kg SID) and underwent surgery to excise the abscess cranial of the prostate. The two ductuli deferentes were attached to this structure. Histopathological analysis revealed this tissue to be embryological remanents or a morphological anomaly with a urogenital origin. Control after 4 weeks showed that the prostate slightly decreased in volume, however, anechogenic cavities where still present of which one increased in volume. The alopecia was still present as well, both probably due to a prolonged action of the estrogens. Conclusion. Even though injectable estrogen preparations are not longer available in veterinary medicine in Europe, a thorough anamnesis towards other exogenous estrogen sources is still necessary and can reduce the number of supplementary exams performed. [less ▲]

The Effect of Cooking Food on Fires from Solid Fuels vs Electricity, Gas and Paraffin on Levels of Dioxins and PCBs in a South African Population

in Organohalogen Compounds (2012, August), 74

The effect of homologous prostatic fluid on the parameters of dog semen extended and frozen in Tris-egg yolk and INRA Freeze: preliminary results

in Reproduction in Domestic Animals (2012, August), 47(s4), 431

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a ... [more ▼]

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a number of detrimental effects on the frozen-thawed canine semen. The aim of this study was to compare the effect of a LDL commercial extender used for stallion semen – INRA FreezeÒ (IMV Technologies, L¢Aigle, France) with a standard extender for dog semen containing Tris, fructose, glycerol and 20% egg yolk (TFG-EY). Since there are some conflicting reports concerning the effect of prostatic fluid on sperm function, we also investigated if the effect of post-thaw dilution with autologous prostatic fluid on viability, motility and acrosome status of frozen-thawed canine spermatozoa is correlated with the composition of the extender. Semen was collected from six Beagle dogs and each sperm rich fraction was divided in two parts: one was diluted with INRA FreezeÒ and the other with TFG-EY and frozen with the same protocol. The third fraction of each ejaculate was collected separately, centrifuged at 1118 · g for 10 min and the supernatant was frozen at )18°C until use. For each extender, two straws were thawed: one straw was diluted 1:2 with autologous prostatic fluid, while the other was not diluted at all. Motility (CASA, IVOS; Hamilton Thorne, USA), viability and acrosome status (Guava EasyCyteÒ flow cytom- eter; IMV Technologies, L¢Aigle, France), morphology (Diff-Quick stain) were assessed at 5 min, 1 h and 2 h post-thaw (T0, T1, T2). Statistical analyses were performed with IBM SPSS software (ver. 19 for Windows; IBM, New York, NY, USA). The results are presented as mean values and a p value <0.05 was considered statistically significant. Viability, total and progressive motility and the percentage Ó 2012 Blackwell Verlag GmbH 432 Abstracts of intact acrosomes were higher (p < 0.05) for the TFG-EY group with or without the addition of the prostatic fluid. The results for motility in the INRA Freeze group were dramatically low compared to the TFG-EY group. There were no significant differences regarding the morphology of fresh and frozen semen for any of the four groups. Addition of prostatic fluid for the TFG-EY group significantly reduced the total and progressive motility at T0, T1 and T2 (p < 0.05), but showed no influence for the INRA Freeze group. This data show that the effect of prostatic fluid regarding motility differs between the two extenders. This may illustrate either an inhibitory effect of some components of the INRA FreezeÒ on the noxious effects of the prostatic fluid or conversely an enhancing effect of the TFG-EY on those. Alternatively, it could be that low values obtained with INRA FreezeÒ did not permit highlighting the effects of prostatic fluid. [less ▲]

Analytical criteria for use of MS/MS for determination of dioxins and dioxin-like PCBs in feed and food

in Organohalogen Compounds (2012, August), 74

Analysis of PCDD/Fs and dioxin-like PCBs in sewage sludges and general bio-wastes available for agricultural land application in Belgium - comparison between GC-IDHRMS and CALUX results

in Organohalogen Compounds (2012, August), 74

A bone remodelling model for long term orthodontic tooth movement.

in Journal of Biomechanics (2012, July), 45(Supplement 1), 180

The situation of gerontechnology in Belgium

in GERONTECHNOLOGY (2012, June 26), 11(2), 269-270

Granulomatous meningo-encephalitis caused by Toxoplasma gondii in three bulls, a possible explanation for unexplained sporadic bovine meningo-encephalitis

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

A very high prevalence of vitamin D inadequacy combined with low dietary calcium intake is found in European postmenopausal women

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 716

Investigation of body condition score and insulin sensitivity (RQUICKY) in downer cow syndrome

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

Perception, knowledge and use by general practitioners of Belgium of the FRAX tool

in Annals of the Rheumatic Diseases (2012, June), 71(Suppl.3), 716

Investigation of sudden death of calves after epileptic seizures due to hypomagnesaemia and hyperparathyroidism

in Revista Portuguesa de Buiatria (2012, June), (Special Edition),

Efficacy of a strontium ranelate 2 G/vitamin D3 1000 UI combination on the correction of vitamin D insufficiency

in Osteoporosis International (2012, May), 23(S2), 225

Automatic real-time collection of RCS of airplanes in a real bistatic low-frequency configuration using a software defined passive radar based on illuminators of opportunity

in Proceedings of the 2012 IEEE Radar Conference (2012, May)

DREAM 2012: DEVELOPMENT OF LAPAROSCOPIC SURGERY AT THE UNIVERSITY HOSPITAL OF KINSHASA, DRC

in Acta Chirurgica Belgica (2012, May), 112(3), 8240

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that ... [more ▼]

Objectives: The technical nature of laparoscopy, and the required specific laparoscopic tools and medical skills, may render this approach difficult in developing countries. We hypothesized that laparoscopy may be developed in the Cliniques Universitaires de Kinshasa (CUK), and may be cost-effective. The final aim of this program is to bring the benefits of laparoscopy to the DRC population, by allowance of adequate training on the UNIKIN personnel, including anaesthetists, surgeons and nurses, who in the future will have to locally form the DRC medical and nursery students. Methods: With the financial support from Wallonie-Bruxelles International (WBI), a complete CUK team, including a surgeon (2 years training in Belgium), an anaesthetist and nurses, were trained in Belgium and then afterwards in DRC. The laparoscopic equipment was sent to Kinshasa, and three theoretical and practical missions of Belgian teams were organised. Results: Over a 2 year period, 116 laparoscopic procedure were performed, including 32 appendectomies, 41 cholecystectomies, 11 hernia repairs, 9 laparoscopy explorations for peritoneal carcinoma assessment and biopsy, 8 procedures for catheter of dialysis peritoneal, 5 gynecologics procedures, and 10 other miscellaneous procedures. Conclusions: A joined approach, taking into account on one hand the training of the skills locally trained to adapt itself to some difficulties, on the other hand institutions of scientific support and a real program and local will of development of this new procedure are the wages of development, accessibility and durability of such news approach in developing countries. All University and non-University team willing to join such a project are welcome. [less ▲]

INTRAPERITONEAL ADHESIONS AFTER OPEN OR LAPAROSCOPIC ABDOMINAL PROCEDURE: AN EXPERIMENTAL STUDY IN THE RAT

in Acta Chirurgica Belgica (2012, May), 112(3), 8546

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 ... [more ▼]

Objectives: Adhesion formation is common after abdominal surgery. The incidence and severity of adhesion formation following open or laparoscopic surgery remain controversial. The role of CO2 pneumoperitoneum is also largely discussed. This study aimed to compare adhesion formation following peritoneal injury by electrocoagulation performed through open or laparoscopic procedures in a rat model. Methods: Sixty male rats were randomized to undergo a 1.5cm peritoneal injury with unipolar cautery under general anesthesia: open surgery (group A, n=20), laparoscopic surgery with CO2 pneumoperitoneum (group B, n=20) and laparoscopic surgery with air pneumoperitoneum (group C, n=20). Duration of the procedures was fixed at 90 minutes in all groups, and pneumoperitoneum pressure at 10mmHg. Ten days later, the animals underwent a secondary laparotomy to score peritoneal adhesions using qualitative and quantitative parameters. Results: Forty-five rats developed at least one adhesion, respectively 95% in group A, 83% in group B and 55% in group C (P<0.01; Group C vs Group A, P<0.01). According to number, thickness, tenacity, vascularization, extent, type, and grading according to Zühkle classification, no significant difference was observed between groups A and B. The distribution of adhesions after open surgery was significantly different than after laparoscopic surgery (P<0.001). Interestingly, group C rats developed significantly less adhesions at the traumatized site, and their adhesions had less severe qualitative scores compared to open surgery (P<0.01). Conclusions: In this animal model, CO2 laparoscopic surgery did not decrease the formation of postoperative adhesion, compared to open surgery. The difference with the animals operated with air pneumoperitoneum emphasizes the role of CO2 in peritoneal injury leading to adhesion formation. [less ▲]

INTRA-TUMORAL HETEROGENEITY AND RATIONAL SELECTION OF ANTIGENS FOR TARGETED THERAPY OF LIVER METASTASES

in Acta Chirurgica Belgica (2012, May), 112(3), 8953

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer ... [more ▼]

Objectives: Targeted therapies of liver metastases are gaining a major stake in current and future treatment options. However, the malignant lesions are heterogeneous in nature offering niches for cancer cells causing treatment resistance and relapse. Therefore, a rational strategy is needed to select targetable antigens that would overcome this intra-tumoral heterogeneity. Methods: After ethical committee approval, 48 fresh liver metastases of colorectal origin were prospectively collected from patients undergoing liver resection. Here we macroscopically divided the lesion in different zones and generated a unique quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. Particular focus was laid on accessible proteins, a protein subclass comprising cell membrane associated and extracellular proteins. Accordingly, the tissues were ex-vivo biotinylated, affinity purified and analyzed for each zone separately using nano-UPLC-MSe proteomics technique. In total over 1500 unique proteins were statistically divided into different patterns of expression. Results: We have generated a quantitative picture of the proteome heterogeneity in colorectal carcinoma liver metastases. The study offers insight into novel targets but also antigens against which the antibodies are already involved in clinical trials or treatment of liver metastases. Extensive clustering and validation experiments highlight novel markers that offer the potential to homogeneously cover the metastatic lesion and become better targets. Conclusions: Two such antigens, LTBP2 and TGFBI were selected for functional analysis in colorectal carcinoma cells. In vitro and in vivo experiments showed that in particular TGFBI is relevant for migration and proliferation capacity of colorectal cancer cells. The suppression of this protein led to significant inhibition of tumor growth, crystalizing it as bona fide target for the development of anti-metastases therapies. [less ▲]

Liver resection and vascular reconstruction under protective intraportal cooling during a total liver clampage

in Acta Chirurgica Belgica (2012, May), 112(3), 10216

Étalonnage dynamique des capteurs piézoélectriques pour la mesure de haute pression balistique

in 4ème Conférence Internationale de métrologie - CAFMET2012 (2012, April 24)

Feasibility of scanning fetal anatomy in the first trimester of gestation

in European Federation of Societies for Ultrasound in Medicine and Biology (2012, April 22)

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and ... [more ▼]

Objectives: This study aims to evaluate the feasibility of performing an anatomy scanned for fetal abnormalities at the time of nuchal translucency (NT) measurement in Vietnamese population Material and methods: In a prospective study, 2500 singleton pregnancies measured fetal NT and scanned structural anatomy in the first trimester; then checked fetal morphology systematically at 18-24 weeks and followed up to their delivery. According to ultrasonographic abnormalities and amniocentesis, we evaluated the detection rate of ultrasound for aneuploidy in the first and second trimester of gestation. Results: The sensitivity and specificity of ultrasound for aneuploidy was 17.2% and 99.7%, respectively. All 13 cases of fetal ultrasonographic anomalies related significantly with an increased NT (≥ 2.4mm) (p < 0.001). 30.8% of these abnormalities that included cystic hygroma, omphalocele and holoprosencephaly were detected at 11-13.6 weeks’ gestation. In the second trimester scanning, 69.2% of major structural anomalies found and the detection rate of central nervous system malformation, abnormal heart, abdominal wall defect, face anomaly and skeletal deformities were 85.7%, 100%, 66.7%, 50% and 75%, alternatively. Conclusion: Scanning of fetal anatomy at the time of NT measuring is useful to detect fetal abnormalities, especially aneuploidy in Vietnamese pregnancies. [less ▲]

THE RESPONSE OF ACETOBACTER SENEGALENSIS TO STRESSORS: A STUDY TOWARDS IMPROVEMENT OF VINEGAR STARTER PRODUCTION

in Acetic Acid Bacteria (2012, April)

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular ... [more ▼]

Acetic acid bacteria encounter various harsh conditions during acetic acid fermentation. Ethanol as the main substrate and acetic acid as the major product at low pH can influence deeply on the cellular functions of acetic acid bacteria. In previous studies in CWBI, Acetobacter senegalensis was used for production of dried vinegar starters; however the impact of stressors (ethanol and acetic acid) on A. senegalensis remained unclear. In this study, different techniques such as flow cytometry, culturability on solid medium and 2-DiGE were used comparatively to investigate the effect of carbon sources of inoculum media on the tolerance of A. senegalensis to stressors. Analysis of respiration system by flow cytometric methods showed that the presence of 2% (v/v) acetic acid in inoculum medium, in one hand, causes 80% of cells to continue to do respiration after a sudden exposure to 1- 3% (v/v) acetic acid in stress media while 89.7% of cells grown in glucose appeared as dead cells after an abrupt exposure to 3%(v/v) of acetic acid. On the other hand, 59.2% and 49.33% of cells grown in the presence of 2% (v/v) of acetic acid could maintain their entire membrane integrity after exposure to 1% and 3% (v/v) of acetic acid, respectively. Inoculum medium contained 5% (v/v) of ethanol as a carbon source enabled about 90% of cells to keep their growing capacities after a sudden exposure to 3% acetic acid. In contrast, just 40% of cells grown in glucose as a carbon source maintained their culturability on solid medium after exposure to 1% acetic acid. A similar profile of culturability was observed for the cells grown in 5% (v/v) ethanol or 2% (v/v) of acetic acid. A proteomic approach (2-DiGE) was used to analyze proteins expressed in the presence of different carbon sources. Differentially expressed proteins were mainly associated with energy metabolism, carbohydrate metabolisms, folding, sorting and degradation processes. The relative abundance of proteins was extensively different for cell grown in glucose compared with protein contents of cells grown in ethanol or acetic acid. In conclusion, production of a cost effective vinegar starter needs a qualified biomass which tolerates ethanol and acetic acid. Tolerance of A. senegalensis to acetic acid depends to a great extent on the composition of the medium which cells grow in. In spite of low adaption to acetic acid for cell grown in glucose, using ethanol or acetic acid in inoculum media renders a physiological state in A. senegelensis which enables it to cope with higher concentration of acetic acid readily, this biomass has a potential to be used as a starter [less ▲]

Expression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

Expression of specific pathways in the inflamed synovial membrane of osteoarthritis patient: Identification of new potential key intermediates

in Osteoarthritis and Cartilage (2012, April), 20(Supplement 1), 56

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal ... [more ▼]

Purpose: Synovitis is a key factor in osteoarthritis (OA) pathophysiology, contributing to both patient symptoms and disease progression. In this study, using an original methodology comparing normal/reactive (N/R) and inflammatory (I) synovial membranes zones, we investigated the gene expression profiles of synovial cells from these areas and identified differentially regulated pathways. Methods: Synovial cells (SC) were isolated from OA synovial specimens obtained from 12 patients undergoing knee replacement. The inflammatory status of the synovial membrane was characterized by the surgeon according to macroscopic criteria including the synovial vascularization, the villi formation and the hypertrophic aspect of the tissue. At the surgery time, the synovial membrane was dissected and biopsies from N/R and I areas cultured separately for a period of 7 days. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling between N/R and I areas was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class Comparison test between N/R and I areas was based on paired t-test where N/R and I were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Western blot was performed to confirm certain intermediate expression. Results: From among 47000 probes, 17500 were filtered out. Probes with a p-value below than 0.005 were chosen and classified as up- or down-regulated ones. By this way, 896 differentially expressed genes between N/R and I zones were identified. Among these, 576 genes were upregulated (I/NR > 1.5) and 320 downregulated (I/NR < 0.75). With Ingenuity Pathways Analysis, a significant number of the top ranking differentially expressed genes were identified as inflammatory, Wnt and angiogenic pathways. Interleukin (IL)-6 and -8, chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL16) and arachidonate 5-lipoxygenase (ALOX5) were identified as the most upregulated in I zones in the inflammatory pathway. Interestingly, the alarmin S100A9 was found strongly upregulated in this pathway. Wnt5A and LRP (Low density lipoprotein receptor-related protein) 5 were upregulated whereas FZD (Frizzled homolog) 2 and DKK (dickkopf homolog) 3 were downregulated in the Wnt signaling pathway. Finally, stanniocalcin (STC)-1, an intermediate in angiogenesis was identified as the most upregulated gene in I zones compared to N/R zones. This difference of expression was confirmed at the protein level. Conclusions: Using a unique culture system, this study is the first to identify different expression pattern between two areas of synovial membrane from the same OA patient. These differences concern several key pathways involved in OA pathogenesis, i.e. inflammation, Wnt and angiogenesis. This analysis also provided interesting information regarding new potent intermediates as S100A9 and STC-1. They could be potential targets for chondroitin sulfate, one of the most used molecules in the management of OA. New experiments are being perfomed at the moment to elucidate the potential effect of this molecule on these specific differentially expressed genes in the same culture system. [less ▲]

AMICE: erste internationale Modellrechnung der Maas

in Forum für Hydrologie und Wasserbewirtschaftung (2012, March 22), 12(31), 165-170

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den ... [more ▼]

Im Rahmen des AMICE-Projektes wurde eine erste zusammenhängende internationale Modellrechnung für den gesamten Flusslauf der Maas inklusive des Nebenflusses Rur durchgeführt. Dazu wurden die in den Anrainerstaaten Frankreich, Belgien, Deutschland und der Niederlande vorgehaltenen numerischen Modelle verwendet. Die Randbedingungen dieser Modelle wurden in einem iterativen Prozess abgeglichen. Die Ergebnisse der zusammenhängenden Simulation tragen zunächst zu einem besseren Systemverständnis bei. Im weiteren Verlauf des Projektes fließen sie in eine Risikobetrachtung ein und werden zur Bewertung von Maßnahmen zur Reduzierung des Hochwasserrisikos vor dem Hintergrund zu erwartender Änderung der Bemessungsgrößen infolge einer erwarteten Klimaänderung herangezogen. [less ▲]

LA FALSIFICATION DES MEDICAMENTS

in Forum Pharmaceutique (2012, March 16), -(-), 16

Automata-Based Symbolic Representations of Polyhedra

in Lecture Notes in Computer Science (2012, March), 7183

Correlations between 25(OH)D and BMD change in postmenopausal osteoporotic women : secondary analyses of a 1-year trial of weekly alendronate (ALN) plus vitamin D3 5600 IU vs. standard care

in Osteoporosis International (2012, March), 23(S2), 238-239

What are the most important medication attributes for patients with osteoporosis ? Results from a qualitative study

in Osteoporosis International (2012, March), 23(S2), 81-82

Denosumab treatment of postmenopausal women with osteoporosis for 6 years : results from the first 3 years of the freedom extension

in Osteoporosis International (2012, March), 23(S2), 76

Perception, knowledge and use by general practitioners of Belgium of the FRAX tool

in Osteoporosis International (2012, March), 23(Suppl. 2), 363-364

Prediction of future knee surgery in patient with osteoarhtritis by the use of a new definition of x-ray progression

in Osteoporosis International (2012, March), 23(Suppl. 2), 363

Radiological and clinical profil of osteoarthritic patients undergoing of total joint replacement

in Osteoporosis International (2012, March), 23(Suppl. 2), 129-130

Relationships between changes in bone mineral density and vertebral fractures incidence: an analysis of the last 2 years of a 10-year treatment with strontium ranelate

in Osteoporosis International (2012, March), 23(Suppl. 2), 362

Cost-effectiveness of bazedoxifene compared with raloxifene in the treatment of postmenopausal osteoporotic women

in Osteoporosis International (2012, March), 23(Suppl. 2), 312-313

Severity of incident vertebral fracture and future fracture risk: a 3-year prospective study

in Osteoporosis International (2012, March), 23(Suppl. 2), 60-61

Establishment of parathyroid hormone (PTH) reference on 10 different assay kits: impact of the recruitment of the population

in Osteoporosis International (2012, March), 23(Supplement 2), 360

A very high prevalence of vitamin D inadequacy combined with low dietary calcium intake is found in European postmenopausal women

in Osteoporosis International (2012, March), 23(Suppl. 2), 361