References of "Scientific congresses and symposiums"
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See detailHigh-Precision Spectroscopy of Pulsating Stars
Aerts, C.; Hekker, S.; Desmet, M. et al

in Precision Spectroscopy in Astrophysics, Proceedings of the ESO/Lisbon/Aveiro Conference held in Aveiro, Portugal, 11-15 September 2006. (2008)

We review methodologies currently available to interprete time series of high-resolution high-S/N spectroscopic data of pulsating stars in terms of the kind of (non-radial) modes that are excited. We ... [more ▼]

We review methodologies currently available to interprete time series of high-resolution high-S/N spectroscopic data of pulsating stars in terms of the kind of (non-radial) modes that are excited. We illustrate the drastic improvement of the detection treshold of line-profile variability thanks to the advancement of the instrumentation over the past two decades. This has led to the opportunity to interprete line-profile variations with amplitudes of order m/s, which is a factor 1000 lower than the earliest line-profile time series studies allowed for. [less ▲]

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See detailTowards an iron magneto-optical trap
Krins, Stéphanie ULg; Saint-Georges, P.; Bastin, Thierry ULg

Poster (2008)

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Peer Reviewed
See detailIn Anticipation of the Loop : Stein’s Four Saints
Delville, Michel ULg

Conference (2007, December 21)

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See detailLes applications de la logique modale dans l’étude des modalités linguistiques
Polis, Stéphane ULg

Conference (2007, December 20)

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See detailUsing survey data to resolve the exchange risk exposure puzzle
Muller, Aline ULg; Jongen, Ron; Verschoor, Willem

Conference (2007, December 20)

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See detailLes temporalités véridictoires dans la photo de sport
Dondero, Maria Giulia ULg

in Nouveaux Actes Sémiotiques (2007, December 14)

La première partie de cet article approche le problème de la véridiction de l’image et du jugement épistémique en prenant en considération la relation entre la textualité photographique et ses pratiques ... [more ▼]

La première partie de cet article approche le problème de la véridiction de l’image et du jugement épistémique en prenant en considération la relation entre la textualité photographique et ses pratiques de production et de réception. On porte l’attention sur le fait que la textualité, qu’elle soit ou non photographique, ne peut pas être étudiée uniquement à partir de ses configurations plastiques et figuratives et de la constatation d’une cohérence sémiotique interne à l’image. En effet sa signification dépend fortement de ses pratiques productives (ou mieux encore de ce qu’on connaît des pratiques productives), de ses dispositifs « présentationnels » (comme par exemple l’album de famille, les affiches, la presse, Internet, etc.), de ses statuts (scientifique, documentaire, testimoniale, artistique, publicitaire) et de son histoire interprétative. La seconde partie de l’article analyse les stratégies énonciatives d’un reportage sportif, les Jeux Olympiques d’été de 2004 à Athènes. Le corpus d’images pris en considération construit différentes configurations de point de vue et diverses manières de mettre en présence de l’observateur le corps de l’athlète : des corps en action, des corps fatigués, des corps en équilibre, des corps concentrés, des corps tendant vers le but, enfin des corps avec lesquels nous construisons une « relation inhérente » grâce à une saisie analogisante. Nous avons ainsi individualisé trois types de mise en présence du corps et trois types de temps présent. Dans le premier type de présent, appelé dimensionné, le mouvement corporel entre en résonance avec la « traînée » des moments retenus et la « protension » de ceux qui se profilent comme possibles ; le second type est un présent d’appariement : dans ce deuxième cas l’exploration perceptive du photographe et du récepteur de l’image se coordonnent avec l’alternance des « points d’attaque » synchronisés des corps dansants. Le troisième type de présent, intervalle-échantillon, enfin, construit l’athlète en tant qu’identité et conscience en acte. [less ▲]

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See detailAnalyse pluviométrique au Niger : récentes modifications et impacts environnementaux. Focus sur la région de Gouré, Niger oriental.
Ozer, Pierre ULg

Conference (2007, December 13)

Depuis la fin des années 1960, la région sahélienne est touchée par une terrible sécheresse. Cet article analyse les variations climatiques enregistrées dans la région de Gouré, Niger oriental, entre 1936 ... [more ▼]

Depuis la fin des années 1960, la région sahélienne est touchée par une terrible sécheresse. Cet article analyse les variations climatiques enregistrées dans la région de Gouré, Niger oriental, entre 1936 et 2003. Il apparaît que la zone étudiée est sortie de la « Grande Sécheresse » à la fin des années 1980. Cependant, les conséquences de ce retour à des conditions plus humides ne se seraient pas accompagné d’une amélioration significative de la longueur de la saison des pluies. [less ▲]

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See detailThe role of Constitutional Courts as positive framework-legislators
Behrendt, Christian ULg

Conference (2007, December 11)

Conference in comparative constitutional law given in 2007 at the Law Faculty of the Erasmus University of Rotterdam (Erasmus Law School), and exposing the results of my PhD research, published in 2006 ... [more ▼]

Conference in comparative constitutional law given in 2007 at the Law Faculty of the Erasmus University of Rotterdam (Erasmus Law School), and exposing the results of my PhD research, published in 2006, on the role of Constitutional Courts as framework-legislators. [less ▲]

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See detailDecontamination of Resistant Emerging Pathogens
Melin, Pierrette ULg

Conference (2007, December 11)

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See detailLes glomérulonéphrites
Krzesinski, Jean-Marie ULg

Conference (2007, December 11)

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See detailCauses et mécanismes de la perte de l’aptitude au fractionnement du maïs séché à haute température
Malumba Kamba, Paul ULg; Deroanne, Claude; Masimango, Thaddée et al

Poster (2007, December 10)

Le séchage par entrainement d’air chaud dénature les principales familles des protéines du maïs, ce qui entraine la diminution de leur solubilité. Les températures élevées de séchage induisent également ... [more ▼]

Le séchage par entrainement d’air chaud dénature les principales familles des protéines du maïs, ce qui entraine la diminution de leur solubilité. Les températures élevées de séchage induisent également une diminution de l’aptitude à l’imprégnation d’eau des grains lors du trempage des maïs avec pour conséquence la diminution du ramollissement des grains, ce qui occasionne des moutures imparfaites et une réduction de la libération des granules d’amidon lors des phases de séparation de l’amidon et des protéines. La fonction logistique à deux asymptotes permet une meilleure prévision des rendements d’extraction de l’amidon et des quantités des glutens récoltées au tamisage en fonction de la température du séchage. [less ▲]

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See detailNumerical and experimental characterization of flow in a radial flow cell with aspect ration one.
Detry, Jean; Jensen, B.; Koitzsch, R. et al

Conference (2007, December 10)

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See detailSalt, hypertension and the kidneys
Krzesinski, Jean-Marie ULg

Conference (2007, December 07)

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See detailConciliation qualité de vie au travail et qualité de vie en général
Etienne, Anne-Marie ULg

Conference (2007, December 07)

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See detailRisque cardiovasculaires chez les jeunes étudiants
Krzesinski, Jean-Marie ULg

Conference (2007, December 07)

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See detailLes apories du matérialisme dialectique
Seba, Jean-Renaud ULg

Conference (2007, December 07)

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See detailFrom the numerisation of the Soil Map of Wallonia to a real information and management tool
Legrain, Xavier ULg; Bah, Boubacar Billo ULg; Veron, Philippe et al

Poster (2007, December 05)

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See detailFunctional Characterization of Snail2 Mediated E-Cadherin repression
Molina Ortiz, Patricia ULg; MacPherson, Matthew; Cano, Amparo et al

Poster (2007, December 02)

Snail2, also called Slug, is a member of the Snail-family of zinc-finger transcription factors that plays a significant role both during development and carcinogenesis, by controlling epithelial ... [more ▼]

Snail2, also called Slug, is a member of the Snail-family of zinc-finger transcription factors that plays a significant role both during development and carcinogenesis, by controlling epithelial-mesenchymal transition (EMT) processes. Snail2 has been also described as a direct transcriptional repression of E-cadherin during EMT being implicated as a prosurvival factor during tumorogenesis. Snail1 and Snail2 are highly homologous factors, containing a common N-terminal transrepressor domain (SNAG), a C-terminus DNA binding domain of four (Snail1) or five (Snail2) zinc fingers, and a divergent central region, which in Snail2 is formed by a unique domain called `Slug domain´ whose function remains to be elucidated. Snail1 repressor activity has been shown to be dependent on SNAG-mediated interaction with a repression complex formed by the corepressor mSin3a and histone deacetylases 1/2 (HDAC1/2). Importantly Snail1 transcription factor is further regulated through phosphorylation by various kinases. However, at date little is known about the control of Snail2 repressor activity. Here, we present interesting data shedding light into the regulation and function of Snail2 as a E-cadherin repressor. For this purpose we have performed ectopic expression of several Snail2 deletion mutants and examined the contribution of the specific domains to protein stability, localization and E-cadherin repressor activity. These data reveal a key role for the `Slug domain´ to repress E-cadherin expression. Furthermore, in vivo phosphorylation analysis revealed that specific phosphorylation on Snail2 protein is implicated in Snail2 function as a transcriptional repressor whose functional significance is currently being investigated. [less ▲]

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See detailDetección del BLV en muestras de semen de toros seropositivos
Rossich, L.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2007, December 02)

Detección del BLV en muestras de semen de toros seropositivos Rossich Luciano1; Gutierrez Geronimo1; Rodriguez Sabrina1; Lefebvre Eduardo2; Trono Karina1; Dus Santos Maria Jose1. (1) Instituto de ... [more ▼]

Detección del BLV en muestras de semen de toros seropositivos Rossich Luciano1; Gutierrez Geronimo1; Rodriguez Sabrina1; Lefebvre Eduardo2; Trono Karina1; Dus Santos Maria Jose1. (1) Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. (2) Centro de Inseminación Artificial La Elisa. Argentina. La principal ruta de transmisión del Virus de la Leucosis Bovina (BLV) es horizontal y esta dada por la exposición directa de fluidos biológicos contaminados con linfocitos infectados; principalmente sangre. A pesar de que antígenos virales y ADN proviral han sido identificados en semen, leche y calostro; la transmisión natural a través de estas secreciones no ha sido demostrada. El impacto sanitario y económico de la infección por BLV esta asociada con la interferencia en el movimiento internacional del ganado vacuno y su germoplasma. Datos experimentales soportan la improbabilidad de que el semen congelado de toros seropositivos para BLV puedan infectar vacas receptoras, sin embargo, aun hoy existen restricciones para la comercialización de semen de animales infectados. Dado que no existe vacuna ni tratamiento aplicable, la erradicación y control de la enfermedad esta exclusivamente basada en el diagnóstico temprano y segregación de los animales infectados. En este contexto la especificidad y la sensibilidad del diagnóstico utilizado constituyen un punto crítico. Hemos desarrollado previamente una PCR con alta sensibilidad y especificidad para detectar el genoma de BLV en muestras de semen congelado. El objetivo de este trabajo es el estudio del patrón de excreción natural de BLV en semen. Muestras de semen congelado de 30 toros seropositvos fueron analizados. Fue posible detectar ADN proviral en 144 de 752 muestras. La detección del genoma del BLV ha ocurrido en varias colecciones pero en forma alternada con períodos de no detección. Adicionalmente, la presencia de ARN proviral en sangre y muestras de sangre fue evaluada. [less ▲]

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See detailDetermination du profil en acides gras du lait par spectroscopie moyen infrarouge.
Dehareng, Frédéric; Soyeurt, Hélène ULg; Veselko, Didier et al

in 14 emes Recontres autour des recherches sur les ruminants, Paris, les 5et 6 Decembre 2007 (2007, December 01)

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See detailRadial Velocities and Atmospheric Parameters of Field Stars Suitable for RAVE Tests
Valentini, Marica ULg; Munari, U.; Tantalo, R. et al

in From Stars to Galaxies: Building the Pieces to Build Up the Universe (2007, December 01)

With the Asiago 1.82m telescope + Echelle spectrograph we have obtained high resolution spectra of 25 RAVE targets well distributed in atmospheric parameters. From them we have derived radial velocities ... [more ▼]

With the Asiago 1.82m telescope + Echelle spectrograph we have obtained high resolution spectra of 25 RAVE targets well distributed in atmospheric parameters. From them we have derived radial velocities and T_{eff}, log g, [M/H], V_{rot} sin i to the aim of serving as an external test for corresponding values derived from RAVE spectra. [less ▲]

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See detailTitan Airglow Spectra from Cassini UVIS
Ajello, J. M.; Gustin, Jacques ULg; Stevens, M. et al

Conference (2007, December 01)

We present the first UV airglow observations of Titan's atmosphere by the Ultraviolet Imaging Spectrograph (UVIS) on Cassini. The known UV emissions of Titan have been examined with higher spectral ... [more ▼]

We present the first UV airglow observations of Titan's atmosphere by the Ultraviolet Imaging Spectrograph (UVIS) on Cassini. The known UV emissions of Titan have been examined with higher spectral resolution (0.4 nm FWHM) by the Cassini UVIS than in the past by the Voyager Ultraviolet Spectrometer (UVS) (3.0 nm FWHM). The UVIS observations, confirming Voyager UVS results, have shown that molecular nitrogen is the major constituent of the upper atmosphere of Titan. Using one spectral channel in the EUV from 56--118 nm and one in the FUV from 112--191 nm, the UVIS observed the disk on 13 December, 2004. The combined EUV and FUV spectral region is a probe of the stratosphere-mesosphere-thermosphere-exosphere region from about 300--2000 km. The EUV spectrum consists of three band systems of N2 (b {1}Πu, b' {1}Σu+, c{4'} {1}Σ{u+}--X {1}Σ{g+}), while the FUV spectrum consists of one N2 (a {1}Πg--X {1}Σ{g+}). The UVIS observations reveal that the c{4'}(0)--X(0) vibrational band near 95.8 nm is suppressed, and that N \sc{i} multiplets near 95.32 and 96.45 nm are present instead. Magnetospheric particle excitation is weak on this orbit, since the nightside EUV spectrum shows no observable N2 emission features and only H Lyman-β. The absence of significant darkside emission demonstrates that nitrogen emissions are predominantly excited on this orbit by photoelectrons near 900 km in the thermosphere. Above 145 nm most of the observed signal is due to sunlight reflected by N2 in the Titan mesosphere-stratosphere and modified by aerosol and hydrocarbon absorption. Mixing ratios of C2H2, C4H2, C2H4 and tholins have been derived from the reflected sunlight emission between 145 and 190 nm, using a Rayleigh scattering model. Assuming that the energy deposition at these wavelengths occurs near 300 km, these mixing ratios are in good agreement with recent photochemical models and previous Voyager observations in the IR. We also present the first geometric albedo measurement of Titan from 150--190 nm. [less ▲]

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See detailARCS: the Asiago Red Clump Spectroscopic Survey
Valentini, Marica ULg; Barbon, R.; Munari, U. et al

in From Stars to Galaxies: Building the Pieces to Build Up the Universe (2007, December 01)

The Asiago Red Clump Spectroscopic survey derives from high resolution optical spectra accurate multi-epoch radial velocities and atmospheric parameters (T_{eff}, log g, [M/H], V_{rot}sin i, [α/Fe], ξ ... [more ▼]

The Asiago Red Clump Spectroscopic survey derives from high resolution optical spectra accurate multi-epoch radial velocities and atmospheric parameters (T_{eff}, log g, [M/H], V_{rot}sin i, [α/Fe], ξ) for a well selected sample of ˜500 equatorial Red Clump stars belonging to the solar neighborhood. These results coupled with astrometric and photometric external data are used in investigation of galactic structure and dynamics. [less ▲]

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See detailMeasured Correlations of Auroral Emissions from Jupiter and Saturn With Solar Wind Variations
Clarke, J. T.; Nichols, J.; Gérard, Jean-Claude ULg et al

Conference (2007, December 01)

An extended set of Hubble Space Telescope (HST) observations of the UV auroral emissions from Jupiter and Saturn has been carried out in three campaigns over Jan.-June 2007. This is by far the most ... [more ▼]

An extended set of Hubble Space Telescope (HST) observations of the UV auroral emissions from Jupiter and Saturn has been carried out in three campaigns over Jan.-June 2007. This is by far the most extensive series of remote high resolution imaging of planetary aurora to date, and provides new physical insight into the cause and effect relationships governing the controlling processes for the giant planet auroral emissions. Simultaneous in situ measurements of local solar wind and magnetospheric plasma conditions have been made during two of these campaigns by Cassini at Saturn in Jan. 2007 and by the New Horizons mission approaching Jupiter in Feb. 2007. The UV auroral emission brightness and distributions have also been compared with estimates of the solar wind conditions near each planet extrapolated from near-Earth measurements, which can be verified by comparison with Cassini and New Horizons in situ data. It has been found that there is a good correlation at both planets between total auroral power and solar wind dynamic pressure, at least for the major solar wind disturbances arriving at each planet. At the same time, the nature of the auroral brightenings differs between Jupiter and Saturn, and the source regions of auroral activity are quite different in the two magnetospheres. In this presentation, the HST and solar wind data and the nature of the correlations will be presented. The physical significance of the correlations will be discussed, based on the much denser set of measurements now available. [less ▲]

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See detailAuroral Movies and Spectroscopy from Cassini UVIS
Pryor, W. R.; West, R.; Stewart, I. et al

Conference (2007, December 01)

Cassini's Ultraviolet Imaging Spectrograph (UVIS) has completed three years of study of Saturn's atmosphere and auroras. Two long slit spectral channels are used to obtain EUV data from 56.3-118.2 nm and ... [more ▼]

Cassini's Ultraviolet Imaging Spectrograph (UVIS) has completed three years of study of Saturn's atmosphere and auroras. Two long slit spectral channels are used to obtain EUV data from 56.3-118.2 nm and FUV data from 111.5-191.3 nm. 64 spatial pixels along each slit are combined with slit motion to construct spectral images of Saturn. Auroral emissions are seen from electron-excited molecular and atomic hydrogen. In 2007 UVIS obtained data with the spacecraft well out of Saturn's ring plane, permitting us to create images, spectra, and at times movies. We will present an auroral movie from 2007-145 that has been processed to remove flat-fielding artifacts and deconvolved to remove scattering along the slit. The movie shows near co- rotation of N polar auroral features with the planet's rotation. An auroral oval is present. The oval appears doubled on the midnight side. Other images from this year show emissions inside the auroral oval. We will discuss these images and their spectra. Additional images and movies are planned in coming months. [less ▲]

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See detailNonlinear Aeroelastic vibrations and galloping of iced conductor lines under wind.
Shklyarchuk, Fedor; Danilin, Alexander; Lilien, Jean-Louis ULg et al

in Proceedings of the 7th International Conference on Cable Dynamics (2007, December)

galloping model for one span single conductor dead ended.

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See detailConsensus under general convexity
tuna, S. E.; Sepulchre, Rodolphe ULg

in Proceedings of the 46th IEEE Conference on Decision and Control (2007, December)

A method is proposed to characterize contraction of a set through orthogonal projections. For discrete-time multi-agent systems, quantitative estimates of convergence (to a consensus) rate are provided by ... [more ▼]

A method is proposed to characterize contraction of a set through orthogonal projections. For discrete-time multi-agent systems, quantitative estimates of convergence (to a consensus) rate are provided by means of contracting convex sets. Required convexity for the sets that should include the values that the transition maps of agents take is considered in a more general sense than that of Euclidean geometry. [less ▲]

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See detailColliding-wind binaries as non-thermal X-ray emitters: an observational investigation from XMM-Newton to next generation X-ray observatories
De Becker, Michaël ULg

Poster (2007, December)

The investigation of massive stars in the radio domain revealed about 25 years ago that some of them are synchrotron emitters, showing that these objects are able to accelerate particles up to ... [more ▼]

The investigation of massive stars in the radio domain revealed about 25 years ago that some of them are synchrotron emitters, showing that these objects are able to accelerate particles up to relativistic velocities. In this context, non-thermal emission processes such as inverse Compton scattering are expected to be at work in the high-energy domain. For this reason, an observational campaign devoted to the X-ray investigation of non-thermal radio emitters has been carried out with XMM-Newton. However, considering the rather strong thermal X-ray emission from these systems below 10 keV, XMM-Newton does not appear to be the ideal observatory to detect their putative non-thermal X-rays. As a consequence, the advent of next generation X-ray observatories with a bandpass extending significantly above 10 keV (SIMBOL-X, XEUS, NEXT) is expected to provide important results related to the non-thermal high-energy emission from colliding-wind binaries and their capability to accelerate particles. [less ▲]

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See detailAn Arbitrary Lagrangian Eulerian Formulation for the Numerical Simulation of Forming Processes
Boman, Romain ULg; Papeleux, Luc ULg; Ponthot, Jean-Philippe ULg

in International Workshop 2007: Advancements in Design Optimization of Materials, Structures and Mechanical Systems (2007, December)

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See detailImmobilisation de la lipase de Yarrowia lipolytica.
Alloue, W. A. M.; Destain, Jacqueline ULg; El Mejdoub, Thami ULg et al

Poster (2007, December)

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See detailStabilization of Collective Motion in Three Dimensions : A Consensus Approach
Scardovi, Luca; Leonard, Naomi; Sepulchre, Rodolphe ULg

in Proceedings of 46th IEEE Conference on Decision and Control (2007, December)

This paper proposes a methodology to stabilize relative equilibria in a model of identical, steered particles moving in three- dimensional Euclidean space. Exploiting the Lie group structure of the ... [more ▼]

This paper proposes a methodology to stabilize relative equilibria in a model of identical, steered particles moving in three- dimensional Euclidean space. Exploiting the Lie group structure of the resulting dynamical system, the stabilization problem is reduced to a consensus problem on the Lie algebra. The resulting equilibria correspond to parallel, circular and helical formations. We rst derive the stabilizing control laws in the presence of all-to-all communication. Providing each agent with a consensus estimator, we then extend the results to a general setting that allows for unidirectional and time-varying communication topologies. [less ▲]

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See detailPredatory boreholes in Tournaisian (Lower Carboniferous) spiriferid brachiopods
Mottequin, Bernard ULg; Sevastopulo, George

in Budd, G. E.; Streng, M.; Daley, A. C. (Eds.) et al Programme with abstracts of the 51st Palaeontological Association Annual Meeting (2007, December)

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See detailApplication de la GnRH au traitement de l'infertilité chez la vache
Hanzen, Christian ULg

Conference (2007, December)

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See detailDécouverte d'une statuette/récipient rubanée à Alleur (Hesbaye liégeoise, Belgique)
Delye, Emmanuel ULg; Otte, Marcel ULg

in Archaeologia Mosellana (2007, December), 7

Une statuette modelée, réduite par cassure à la base d'un torse, humain ou animal, fut découverte à Alleur (Liège, Belgique) dans un contexte Rubané (fin VIe millénaire avant J.-C.). Les comparaisons de ... [more ▼]

Une statuette modelée, réduite par cassure à la base d'un torse, humain ou animal, fut découverte à Alleur (Liège, Belgique) dans un contexte Rubané (fin VIe millénaire avant J.-C.). Les comparaisons de cet objet rituel portent vers l'Europe centrale (Allemagne, Hongrie). [less ▲]

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See detailNumerical and analytical investigations on iron columns reinforced by FRP under axial compression
Ly, Dong Phuong Lam ULg; Rondal, Jacques ULg; Jaspart, Jean-Pierre ULg

in Smith, S. T. (Ed.) Asia-Pacific Conference on FRP in Structures-APFIS07 (2007, December)

This paper presents numerical and analytical investigations on iron columns under axial compression, strengthened with high modulus carbon fibre reinforced polymer (FRP) sheets. Numerical and analytical ... [more ▼]

This paper presents numerical and analytical investigations on iron columns under axial compression, strengthened with high modulus carbon fibre reinforced polymer (FRP) sheets. Numerical and analytical models are proposed to predict the resistance and stability load of such elements, including effects of section and member geometrical imperfections and of the nonlinear characteristic and the anisotropy in post elasticity of iron material. It is shown that axial resistance and stiffness of iron columns can be increased significantly with the use of longitudinal FRP sheets as a result of the reduction of the column slenderness, but also that transverse FRP sheets should also be used to avoid any local buckling of the longitudinal FRP sheets during loading. [less ▲]

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See detailL’application du principe de subsidiarité au travers de la réglementation européenne et belge de prévention des accidents industriels majeurs
Hanson, Sophie ULg

in actes du colloque Risques industriels majeurs, sciences humaines et sociales (2007, December)

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See detailA new tool for METs monitoring in cardiopulmonary exercise testing. Comparison of the BodyMedia SenseWear Armband with mobile ergospirometry- preliminary results
Bury, Thierry ULg

in Abstract book of the Annual Congress of the Belgium Society of Cardiology (2007, December)

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See detailImplementation and Preliminary Evaluation of an ISP-Driven Informed Path Selection
Saucez, Damien; Donnet, Benoît ULg; Bonaventure, Olivier

in ACM CoNEXT conference (2007, December)

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See detailAlternating Spatial Patterns for Coordinated Group Motion
Swain, Daniel T.; Leonard, Naomi Ehrich; Couzin, Iain D. et al

in Proceedings of the 46th IEEE Conference on Decision and Control (2007, December)

Abstract—Motivated by recent observations of fish schools, we study coordinated group motion for individuals with oscillatory speed. Neighbors that have speed oscillations with common frequency, amplitude ... [more ▼]

Abstract—Motivated by recent observations of fish schools, we study coordinated group motion for individuals with oscillatory speed. Neighbors that have speed oscillations with common frequency, amplitude and average but different phases, move together in alternating spatial patterns, taking turns being towards the front, sides and back of the group. We propose a model and control laws to investigate the connections between these spatial dynamics, communication when sensing is range or direction limited, and convergence of coordinated group motions. [less ▲]

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See detailAutonomous rigid body attitude synchronization
Sarlette, Alain ULg; Sepulchre, Rodolphe ULg; Leonard, Naomi

in Proceedings of the 46th IEEE Conference on Decision and Control (2007, December)

This paper studies some extensions to the decentralized attitude synchronization of identical rigid bodies. Considering fully actuated Euler equations, the communication links between the rigid bodies are ... [more ▼]

This paper studies some extensions to the decentralized attitude synchronization of identical rigid bodies. Considering fully actuated Euler equations, the communication links between the rigid bodies are limited and the available information is restricted to relative orientations and angular velocities. In particular, no leader nor external reference dictates the swarm's behavior. The control laws are derived using two classical approaches of nonlinear control - tracking and energy shaping. This leads to a comparison of two corresponding methods which are currently considered for distributed synchronization - consensus and stabilization of mechanical systems with symmetries. [less ▲]

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See detailA Hybrid Systems Approach to Trajectory Tracking Control for Juggling Systems
Sanfelice, Ricardo G.; Teel, Andrew R.; Sepulchre, Rodolphe ULg

in Proceedings of the 46th IEEE Conference on Decision and Control (2007, December)

From a hybrid systems point of view, we provide a modeling framework and a trajectory tracking control design methodology for juggling systems. We present the main ideas and concepts in a one degree-of ... [more ▼]

From a hybrid systems point of view, we provide a modeling framework and a trajectory tracking control design methodology for juggling systems. We present the main ideas and concepts in a one degree-of-freedom juggler, which consists of a ball bouncing on an actuated robot. We design a hybrid control strategy that, with only information of the ball’s state at impacts, controls the ball to track a reference rhythmic pattern with arbitrary precision. We extend this hybrid control strategy to the case of juggling multiple balls with different rhythmic patterns. Simulation results for juggling of one and three balls with a single actuated robot are presented. [less ▲]

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See detailSpatial Models of Bistability in Biological Collectives
Paley, Derek; Leonard, Naomi; Sepulchre, Rodolphe ULg et al

in Proceedings of the 46th IEEE Conference on Decision and Control (2007, December)

We explore collective behavior in biological systems using a cooperative control framework. In particular, we study a hysteresis phenomenon in which a collective switches from circular to parallel motion ... [more ▼]

We explore collective behavior in biological systems using a cooperative control framework. In particular, we study a hysteresis phenomenon in which a collective switches from circular to parallel motion under slow variation of the neighborhood size in which individuals tend to align with one another. In the case that the neighborhood radius is less than the circular motion radius, both circular and parallel motion can occur. We provide Lyapunov-based analysis of bistability of circular and parallel motion in a closed-loop system of selfpropelled particles with coupled-oscillator dynamics. [less ▲]

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See detailA kinematic model for hysteretic dissipation of vibration energy for torsional damper and detuner.
Danilin, Alexander; Vinogradov, Alexander; Lilien, Jean-Louis ULg

in Lilien, Jean-Louis (Ed.) Proceedings of the 7th International Conference on Cable Dynamics (2007, December)

A description of the damping properties and model of a low frequency damper for power lines galloping.

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See detailApplication du protocole Ovsynch au traitement du kyste ovarien chez la vache
Hanzen, Christian ULg

Conference (2007, December)

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See detailEtude comparative des performances de production laitiere de brebis de race Mouton Laitier Belge avec celles de brebis de race Lacaune.
Gillon, Alain ULg; Rondia, Pierre; Gengler, Nicolas ULg et al

in 14ièmes Recontres Recherches Ruminants, Paris, les 5 et 6 Décembre 2007 (2007, December)

La Wallonie compte une dizaine de producteurs de lait de brebis avec un cheptel variant de 30 à 250 individus par exploitation, pour la plupart de race « Mouton Laitier Belge » (MLB). Il s’agit d’un ... [more ▼]

La Wallonie compte une dizaine de producteurs de lait de brebis avec un cheptel variant de 30 à 250 individus par exploitation, pour la plupart de race « Mouton Laitier Belge » (MLB). Il s’agit d’un animal de type exclusivement laitier qui s’apparente fortement au mouton laitier frison dont l’effectif s’élève à un millier de têtes. La présence simultanée de brebis Lacaune (LAC) et de brebis MLB sur une même exploitation a permis la comparaison de leurs performances respectives grâce à une maîtrise du facteur « mode de conduite ». L’effectif restreint des populations étudiées constitue une limite à une extrapolation des résultats à l’ensemble des individus des races concernées mais donne néanmoins une certaine indication de leur potentiel de production respectif en région wallonne. [less ▲]

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See detailLa Culture céramique de Terqa au troisième millénaire
Mas, Juliette ULg

Conference (2007, December)

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See detailContribution to the derivation of robustness requirements for steel and composite structures
Jaspart, Jean-Pierre ULg; Demonceau, Jean-François ULg

in Proceedings of Advances in steel structures Conference (2007, December)

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See detailLa démarche expérimentale comme référent externe à l'autorité du professeur ... ou quand le bruit et l'action sont synonymes d'apprentissage et de compétences
Hindryckx, Marie-Noëlle ULg; Daussogne, Christine

in Puzzle (2007, December), 23

Que signifie mener une démarche expérimentale en sciences? En partant d'une mise en situation concrète, nous avons voulu essayer de répondre à quelques questions : que fait-on quand on expérimente en ... [more ▼]

Que signifie mener une démarche expérimentale en sciences? En partant d'une mise en situation concrète, nous avons voulu essayer de répondre à quelques questions : que fait-on quand on expérimente en classe? quel est le rôle de l'élève? de l'enseignant? Quels sont les avantages de travailler par cette méthode? Qui fait "autorité" quand on travaille de telle manière? Les échanges ont été étayés par des résultats d'une recherche-action menée au fondamental. [less ▲]

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See detailFaisabilité de la mise en place d’un outil de mesure de la charge de travail des magistrats destiné au siège : conclusions d’une recherche.
Delvaux, David ULg

Conference (2007, December)

Présentation des résultats d'une recherche mandatée par la Politique Scientifique Fédérale et le Ministère de la Justice et intitulée: "Etude de faisabilité de la mise en place d'un outil de mesure de la ... [more ▼]

Présentation des résultats d'une recherche mandatée par la Politique Scientifique Fédérale et le Ministère de la Justice et intitulée: "Etude de faisabilité de la mise en place d'un outil de mesure de la charge de travail destiné au siège". [less ▲]

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See detailLactation effect on the trace element dynamics in northern elephant seals (Mirounga angustirostris)
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Conference (2007, November 30)

Northern elephant seals, Mirounga angustirostris, are exceptional mammals: females fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Females thus lose ... [more ▼]

Northern elephant seals, Mirounga angustirostris, are exceptional mammals: females fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Females thus lose approximately a third of body mass and produce energy-rich milk with a high fat content. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on days 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. This study confirms that lactation modifies stable nitrogen isotope ratios in tissues, as well as mercury levels in blood from mothers and pups, and highlights the existence of a transplacental and transmammary transfer of mercury in northern elephant seals. Therefore, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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See detailThe Definition of the Concept of Tax
Bourgeois, Marc ULg

Conference (2007, November 29)

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See detailPrinciples of European Contract Law and Consumer Construction Law
Kohl, Benoît ULg

Conference (2007, November 27)

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See detailA dynamic model of an experimental bloom of coccolithophores Emiliania huxleyi
Joassin, Pascal ULg; Borges, Alberto ULg; Chou, Lei et al

Conference (2007, November 27)

A dynamic model has been developed to represent biogeochemical events observed during an experimentally induced bloom of coccolithophores Emiliania huxleyi. This bloom occurred in a mesocosm experiment ... [more ▼]

A dynamic model has been developed to represent biogeochemical events observed during an experimentally induced bloom of coccolithophores Emiliania huxleyi. This bloom occurred in a mesocosm experiment (Bergen 2001 experiment) during which ecosystem development was followed over a 23-days period through changes of the stocks of inorganic nutrients (nitrate, ammonium and phosphate), dissolved inorganic carbon and pCO2, O2 concentration, pigments, particulate organic carbon and nitrogen, dissolved organic carbon, the production of Transparent Exopolymeric Particles (TEP), primary production, alkalinity, calcification and particulate inorganic carbon. The dynamic model is based on unbalanced algal growth and balanced growth for bacteria as described in Van den Meersche et al. (2004). In addition, in order to adequately reproduce the observations, the model has been extended by including an explicit description of calcification, T.E.P production and an enhanced mortality due to viruses. This last process, based on a critical promiscuity between cellular hosts and viral agents, successfully contributed to reproduce the bloom extinction as observed in the mesocosm experiment. This model will be implemented in a coupled physical-biogeochemical model of the Black Sea ecosystem in the framework of the EU Sesame project and in the Gulf of Biscay in the frame of the Belgian PEACE project. [less ▲]

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See detailLateral-torsional buckling of Stainless steel I-beams in case of fire
Vila Real, Paulo; Lopes, Nuno; Da Silva, Luis et al

Conference (2007, November 27)

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See detailTable ronde : «Mort et résurrection de la moelle spinale».
Martin, Didier ULg

Conference (2007, November 25)

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See detailComment mourir et comment ressusciter ?
Martin, Didier ULg

Conference (2007, November 25)

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See detailAnalysis of ordinal longitudinal data
Donneau, Anne-Françoise ULg

Conference (2007, November 24)

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See detailEvolution of Bovine herpesvirus 4: recombination and transmission between African buffalo and cattle
Dewals, Benjamin G ULg; Thirion, Muriel; Markine-Goriaynoff, Nicolas et al

Poster (2007, November 23)

Bovine herpesvirus 4 (BoHV 4) has been isolated from cattle throughout the world, but virological and serological studies have suggested that the African buffalo is also a natural host for this virus. We ... [more ▼]

Bovine herpesvirus 4 (BoHV 4) has been isolated from cattle throughout the world, but virological and serological studies have suggested that the African buffalo is also a natural host for this virus. We have previously found that the Bo17 gene of BoHV-4 was acquired from an ancestor of the African buffalo, probably around 1.5 Myr ago. Analysis of the variation of the Bo17 gene sequence among BoHV-4 strains suggested a relatively ancient transmission of BoHV 4 from the buffalo to the Bos primigenius lineage, followed by a host dependent split between zebu and taurine BoHV 4 strains. In the present study, the evolutionary history of BoHV-4 was investigated by analysis of five gene sequences from each of nine strains representative of the viral species: three isolated from African buffalo in Kenya, and six from cattle from Europe, N. America and India. No two gene sequences had the same evolutionary tree, indicating that recombination has occurred between divergent lineages: six recombination events were delineated for these sequences. Nevertheless, exchange has been infrequent enough that a clonal evolutionary history of the strains could be discerned, upon which the recombination events were superimposed. The dates of divergence among BoHV-4 lineages were estimated from synonymous nucleotide substitution rates. The inferred evolutionary history suggests that African buffalo were the original natural reservoir of BoHV-4, and that there have been at least three independent transmissions from buffalo to cattle, probably via intermediate hosts, and – at least in the case of N. American strains – within the last 500 years. [less ▲]

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See detailInfluence du séchage par entraînement d’air chaud sur l’aptitude au fractionnement par voie humide du maïs
Malumba Kamba, Paul ULg; Deroanne, Claude; Masimango, Thaddée et al

Poster (2007, November 21)

Le séchage par entrainement d’air chaud dénature les principales familles des protéines du maïs, ce qui entraine la diminution de leur solubilité. Les températures élevées de séchage induisent également ... [more ▼]

Le séchage par entrainement d’air chaud dénature les principales familles des protéines du maïs, ce qui entraine la diminution de leur solubilité. Les températures élevées de séchage induisent également une diminution de l’aptitude à l’imprégnation d’eau des grains lors du trempage des maïs avec pour conséquence la diminution du ramollissement des grains, ce qui occasionne des moutures imparfaites et une réduction de la libération des granules d’amidon lors des phases de séparation de l’amidon et des protéines. Une fonction polynomiale de troisième degré est proposée pour prévoir le rendement d’extraction des amidons en fonction des températures appliquées pendant le séchage du maïs de variété Baltimore. [less ▲]

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See detailVers une approche intégrée de la mobilité urbaine
Marchal, Jean ULg; Ndiaye, Alassane-Ballé ULg

in Congrès ISTLS 2007 (2007, November 20)

L’objectif n’est pas de créer un super modèle qui gérerait tous les aspects de la mobilité, mais de faire dialoguer judicieusement les meilleurs modules existants pour chaque aspect de la mobilité ... [more ▼]

L’objectif n’est pas de créer un super modèle qui gérerait tous les aspects de la mobilité, mais de faire dialoguer judicieusement les meilleurs modules existants pour chaque aspect de la mobilité. L’intégration est obtenue par la centralisation via une base de données, ce qui rend possible une vue globale du problème. L'approche repose à la fois sur le traitement et la gestion minutieuse des données grâce à des modules experts dans leur domaine (trafic, émissions polluantes, émissions sonores, aménagement du territoire, juridique, Etc.), en prenant ainsi en compte chaque aspect de la mobilité. L'approche repose à la fois sur le traitement et la gestion minutieuse des données grâce à des modules experts dans leurs domaines (trafic, émission polluante, émission sonore, aménagement du territoire, juridique, etc.), en prenant ainsi en compte chaque aspect de la mobilité. L’approche consiste en l’intégration des données et la communication entre les modules experts existants. L’intégration est obtenue par la centralisation via une base de donnée, ce qui rend possible une vue globale du problème. [less ▲]

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See detailHybridisation efficiency in generating Hybridisation efficiency in generating invasive progeny in the alien Fallopia (Polygonaceae) complex
Saad, Layla ULg; Tiébré, Marie-Solange; Hardy, Olivier et al

Poster (2007, November 16)

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See detailDiet of crustaceans species associated to deep-sea wood falls
Hoyoux, Caroline ULg; Samadi, Sarah; Zbinden, Magali et al

Poster (2007, November 16)

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See detailSurface properties and morophology study of membranes for bone tissue regeneration
Borget, P; Grandfils, Christian ULg; Köttgen, Cindy et al

Conference (2007, November 16)

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See detailOptoacoustic specific detection of prostate cancer using functionalized gold nanorods
Fleron, Maximilien ULg; Schol, Daureen ULg; Greisch, Jean-François et al

Poster (2007, November 16)

A major challenge in prostate cancer oncology is to develop more accurate imaging assessments than those currently available. Indeed, an efficient imaging technique which significantly improves the ... [more ▼]

A major challenge in prostate cancer oncology is to develop more accurate imaging assessments than those currently available. Indeed, an efficient imaging technique which significantly improves the sensitivity and specificity of the diagnostic and predicting the cancer behaviour would be extremely valuable. This project intends to prove the concept of using optoacoustic imaging in combination with biologically functionalized nanoparticles as an integrated biosensor based system for the production of specific and sensitive data for accurate diagnosis of prostate cancer. This concept results on the use of contrast agents which transform an incident luminous energy into local heating inducing a pressure wave detectable by acoustic (echography). For the optoacoustic detection, the nanoparticles used must present a maximum of absorption in the optical transparency window of the human tissues in order to allow their and subsequently the tissue specific excitations while avoiding unwanted destructive energy transfers. According to these characteristics (energy transfer by thermoelastic reaction), rod-like gold nanoparticles (stick form) with a maximum of absorption towards 760 nm were produced by using a “bottom-up” approach with dynamic templates (surfactant). These nanoparticles are then coupled with an antibody directed against the cancerous cells to guarantee the specific detection of the particles. The development of the biosensor is firstly performed to target the Prostate Specific Membrane Antigen (PSMA), a transmembrane protein considered as a suitable biomarker for prostate cancer. Detection and localisation of PSMA on LNCaP fixed cell surface was performed by immunostainning on monolayer cell culture and on spheroid slices. Then, by backscattered electron (BSE) microscopy analysis and two-photon luminescence imaging, detection of nanoparticles on fixed and living cell surface shows the successful binding of the biosensor to the cells expressing PSMA. In prospect, the detection of the biosensor will be tested on spheroids, on human biopsies and finally on in vivo models (mouse xenograft models). [less ▲]

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See detailASSESSMENT OF RETAINED AUSTENITIC PHASE IN INDEFINITE CHILL CAST IRON MICROSTRUCTURE TOWARDS VARIOUS TECHNIQUES
Tchuindjang, Jérôme Tchoufack ULg; Terziev, Liuben; Sinnaeve, Mario et al

Poster (2007, November 16)

Indefinite Chill Cast Irons also called ICDP when used as rolling mill rolls are high alloyed cast iron alloys belonging to the Fe-Cr-C system. ICDP exhibit improved properties regarding hot oxidation and ... [more ▼]

Indefinite Chill Cast Irons also called ICDP when used as rolling mill rolls are high alloyed cast iron alloys belonging to the Fe-Cr-C system. ICDP exhibit improved properties regarding hot oxidation and wear resistances as their microstructure is a mixture of a martensitic with bulky cementite (M3C) and lamellar graphite fully dispersed into the matrix. Mechanical properties involved depend either on the chemical composition of the alloy or the crystallisation behaviour and the heat treatments performed subsequently in order to lead to the desired microstructure. Thus hardenability of the matrix is of great concern as heat treatments sequence is intended to transform austenite in a more stable structure like martensite or even bainite, depending on Cr content. In fact austenite is known to be unstable during hot working conditions as hot temperature and external stresses could yield transformation of this phase into martensite with major volume distortions and even multiple cracks network on the component involved. To avoid such a hitch in service it is quite necessary to make sure all the austenite is completely transformed into martensite while performing subsequent heat treatments like quenching or tempering on the material obtained after casting process. Various techniques are allowable starting from classical ones (X-Ray Diffraction (XRD), Image Analysis, Vibrating Sample Magnetometer (VSM), Dilatometry) to more exotic one like Conversion Electron Mössbauer Spectroscopy (CEMS). From an industrial point of view, it is important to set a fast and reliable method to assess retained austenite presence into ICDP microstructure at the end of any step of the thermomechanical process. The present work is intended to compare different techniques used for retained austenite assessment in ICDP alloys as a first step prior to the calibration of a classical method like dilatometry. This calibration is to be set starting from complex XRD and CEMS spectrometers analyses. Dilatometry seems to overrate retained austenite volume fraction in the as-cast conditions while good agreements are made on the overall batch of techniques when considering heat treated material where retained austenite transformation occurred [less ▲]

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See detailArabidopsis halleri, a model system to understand zinc homeostasis in plants
Hanikenne, Marc ULg; Talke, Ina N.; Hamilton, Christopher ULg et al

Poster (2007, November 16)

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See detailL’évaluation du système de formation
Husson, Jean-François ULg

Conference (2007, November 16)

This contribution was an overview of various evaluation schemes regarding training in various European countires (mainly Britain, The Netherlands and Belgium).

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See detailSurface water protection against diffuse crop protection products release: results of SWAP-CPP project on potatoes (Belgium)
Maugnard, Alexandre; Bodson, Bernard ULg; Goffart, Jean-Pierre et al

Conference (2007, November 15)

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See detailet paroles des travailleurs migrants dans le Delta de la Rivière des Perles
Florence, Eric ULg

Conference (2007, November 15)

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See detailConcevoir des espaces publics durables
Reiter, Sigrid ULg

Conference (2007, November 15)

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See detailLes professionnels du conseil : accomplissement pratiques des politiques de l’autocontrainte
Draelants, Hugues; Orianne, Jean-François ULg

Conference (2007, November 15)

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See detailAssessing the impact of fungal diseases management on crops and environment. Using dynamic models in the case of foliar diseases in wheat
Bancal, Marie-Odile; Roche, Romain; Gabrielle, Benoit et al

Conference (2007, November 13)

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See detailNouvelles directives de l'hypertension artérielle
Krzesinski, Jean-Marie ULg

Conference (2007, November 13)

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See detailLes nouveaux enjeux intellectuels et politiques des sciences sociales
Strivay, Lucienne ULg

Conference (2007, November 12)

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See detailCompiling polymorphic miRNA-target interactions: the Patrocles database.
Hiard, Samuel ULg; Tordoir, Xavier ULg; Coppieters, Wouter ULg et al

Poster (2007, November 12)

Using positional cloning, we have recently identified the mutation responsible for muscular phenotype of the Texel sheep. It is located in the 3’UTR of the GDF8 gene - a known developmental repressor of ... [more ▼]

Using positional cloning, we have recently identified the mutation responsible for muscular phenotype of the Texel sheep. It is located in the 3’UTR of the GDF8 gene - a known developmental repressor of muscle growth - and creates an illegitimate target site for miRNA expressed in the same tissue. This causes miRNA-mediated translation inhibition of mutant GDF8 transcripts which leads to muscle hypertrophy. We followed up on this finding by searching for common polymorphisms and mutations that affect either (i) RNAi silencing machinery components, (ii) miRNA precursors or (iii) target sites. These might likewise alter miRNA-target interaction and could be responsible for substantial differences in gene expression level. They have been compiled in a public database (“Patrocles”: www.patrocles.org), where they are classified in (i) DNA sequence polymorphisms (DSP) affecting the silencing machinery, (ii) DSP affecting miRNA structure or expression and (iii) DSP affecting miRNA target sites. DSP from the last category were organized in four classes: destroying a target site conserved between mammals (DC), destroying a non-conserved target site (DNC), creating a non-conserved target site (CNC), or shifting a target site (S). To aid in the identification of the most relevant DSP (such as those were a target site is created in an antitarget gene), we have quantified the level of coexpression for all miRNA-gene pairs. Analysis of the numbers of Patrocles-DSP as well as their allelic frequency distribution indicates that a substantial proportion of them undergo purifying selection. The signature of selection was most pronounced for the DC class but was significant for the DNC and CNC class as well, suggesting that a significant proportion of non-conserved targets is truly functional. The Patrocles database allowed for the selection of DSP that are likely to affect gene function and possibly disease susceptibility. The effect of these DSP is being studied both in vitro and in vivo. In conclusion, Patrocles-DSP could be widespread and underlie an appreciable amount of phenotypic variation, including common disease susceptibility. [less ▲]

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See detailDetection of BLV in frozen semen samples by PCR assay.
Rossich, L.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2007, November 12)

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar ... [more ▼]

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. (2) Centro de Inseminación Artificial La Elisa. Argentina. Introduction: The major route of transmission of BLV is horizontal by direct exposure to biological fluids contaminated with infected lymphocytes, mainly blood. Although viral antigens and proviral DNA has been identified in semen, milk and colostrums, natural transmission through these secretions has not been demonstrated. The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present[1-6]. Since there is no vaccine or treatment available, eradication and control of the disease is exclusively based on early diagnostic and segregation of infected animals. In this context the specificity and sensitivity of the diagnostic test used is a critical point. We have previously developed a PCR assay with high sensitivity and specificity to detect BLV genome in frozen semen samples. The objective of this work was to study the detection of BLV in semen. Material & methods: Samples: Serum, whole blood and semen samples were obtained from CIALE (Artificial Insemination Centre La Elisa, Capitan Sarmiento, Buenos Aires, Argentina) Serology: The agar gel immunodifussion (AGID) test kit used to detect antibodies to BLV was produced by the Faculty of Veterinary, La Plata University, Argentina. An indirect ELISA using recombinant p24 as antigen was used to detect antibodies to BLV. This assay has been completely developed and standardized in the Institute of Virology, INTA-Castelar, Buenos Aires, Argentina. Detection of proviral DNA: DNA was extracted from PMBCs (purified from whole blood) and semen samples (fresh and straw). PCR assays that amplified a region of genes pol and gag and a nPCR that amplified a region of gene env were developed. Results: Two PCR assays were standardized for detecting BLV genome in semen and PMBC. The limit of detection of viral particles was assessed by the addition of purified pBLV344 (a plasmid containing the complete BLV genome, kindly provided by Dr. Willems, Faculté Universitaire des Sciences Agronomiques, Gembloux, Belgium) to DNA from semen or PMBCs of a seronegative bull. By gag-PCR and pol-PCR, it was possible to detect 60 viral particles, using bromide staining after electrophoresis separation of DNA. In order to increase analytical sensitivity, a nested-PCR was developed which amplified a region of env gene. The n-PCR was able to detected 6 viral particles. Assessment of the limit of detection was highly repetitive under the standardized conditions. Frozen semen samples from 30 seropositive bulls were remitted to the laboratory and analyzed in the period 2005-2007. It was possible to detect proviral DNA in 172 out of 862 samples. BLV genome detection occurred in several collections but in an alternated way with non detection periods. Fresh semen samples, straws and whole blood were also obtained from 5 seronegative and 5 seropositive bulls and tested together for the presence of BLV provirus. Results indicated that while detection of provirus was positive in PMBC from all seropositive bulls, detection of gag, pol and env genes in semen did not occurred in all the samples. Discussions & conclusions: The results obtained suggest that BLV could present an intermittent pattern of excretion. Further studies should be done to confirmed the results obtained and to establish why the presence of BLV provirus in semen is not constant. References : 1. Choi, K, et al. 2002. Vet Diagn Invest 14: 403-406. 2. Kaja, R. and Olson, C. 1982. Theriogenology 18: 107-112. 3. Miller, J. and Van Der Maaten, M. 1979. J Natl Cancer Inst 62; 425-428. 4. Monke, D. 1986. JAVMA 188 (8): 823-826. 5. Romero, C., et al. 1983. Tropical Animal Health and production. 15: 215-218. 6. Straub O. 1982. In: Fourth International Symposium of Bovine Leukosis. The Hague: Martinus Nijhoff Publishers: 299-308. [less ▲]

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