References of "Scientific congresses and symposiums"
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See detailProgramme de revalidation multidisciplinaire post-cancer du sein : analyse des bénéfices éventuels sur la fonction physique et la qualité de vie
LECLERC, Anne-France ULg; FOIDART-DESSALLE, Marguerite ULg; COUCKE, Philippe ULg et al

in AFPSA - 8e congrès francophone, Psychologie de la Santé - Evolutions sociales, innovations & politiques - Résumé des communications (2014, December 16)

Introduction : Le cancer du sein ainsi que ses traitements sont à l'origine de nombreux effets secondaires indésirables. L'objectif de l'étude est de déterminer les bénéfices éventuels, tant sur le plan ... [more ▼]

Introduction : Le cancer du sein ainsi que ses traitements sont à l'origine de nombreux effets secondaires indésirables. L'objectif de l'étude est de déterminer les bénéfices éventuels, tant sur le plan physique que psychologique, d’un programme de revalidation multidisciplinaire chez des femmes ayant été traitées pour un cancer du sein. Méthodologie : 122 patientes ont été recrutées, 61 faisant partie du groupe contrôle et 61 du groupe traité. Celui-ci a bénéficié d’une revalidation de trois mois comprenant un entraînement physique supervisé et diverses sessions psycho-éducatives. Les évaluations, effectuées avant et après la prise en charge, ont inclus des mesures anthropométriques et de composition corporelle, une évaluation des capacités fonctionnelles et différents questionnaires. Résultats : Au terme des trois mois, l’état de santé (qualité de vie), le rôle fonctionnel, l’état émotionnel et les fonctions physiques, cognitives et sociales s’améliorent significativement au sein du groupe traité. Cette observation s’applique également aux symptômes de fatigue, d'insomnie, de douleur, de dyspnée, de perte d'appétit, d’anxiété et de dépression ainsi qu'aux paramètres physiques obtenus par l'intermédiaire des évaluations fonctionnelles. Au sein du groupe contrôle, ces améliorations n’apparaissent pas et une augmentation significative de l'indice de masse corporelle et du pourcentage de graisse corporelle est observée. Conclusion : Cette étude préliminaire montre ainsi la faisabilité et les effets bénéfiques d’une prise en charge multidisciplinaire chez des femmes au terme de leurs traitements pour le cancer du sein. [less ▲]

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See detailPerception du bien-être chez les étudiants de l’Université de Liège
Cloes, Marc ULg; Etienne, Anne-Marie ULg; Maslah, Olfa et al

Conference (2014, December 15)

This study is focused on the analysis of the wellness perception of a representative sample of students of the University of Liege. Findings confirm those of the literature as males at students who ... [more ▼]

This study is focused on the analysis of the wellness perception of a representative sample of students of the University of Liege. Findings confirm those of the literature as males at students who consider to be physically active have higher scores of wellness. The study reveals also that students who are still leaving with their parents have higher scores of wellness. [less ▲]

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See detailMulti-wavelength observations of Jupiter's aurora coordinated with Hisaki and other space telescopes
Kimura, Tomoki; Badman, Sarah; Tao, Chihiro et al

Conference (2014, December 15)

From January to April 2014, two observing campaigns by multi-wavelength remote sensing from X-ray to radio were performed to uncover energy transport process in Jupiter’s plasma environment using space ... [more ▼]

From January to April 2014, two observing campaigns by multi-wavelength remote sensing from X-ray to radio were performed to uncover energy transport process in Jupiter’s plasma environment using space telescopes and ground-based facilities. These campaigns were triggered by the new Hisaki spacecraft launched in September 2013, which is an extremely ultraviolet (EUV) space telescope of JAXA designed for planetary observations. In the first campaign in January, Hubble Space Telescope (HST) made imaging of far ultraviolet (FUV) aurora with a high special resolution (0.08”) through two weeks while Hisaki continuously monitored aurora and plasma torus emissions in EUV wavelength with a high temporal resolution (1 min<). We discovered new magnetospheric activities from the campaign data: e.g., internally-driven type auroral brightening associated with hot plasma injection, and plasma and electromagnetic filed modulations in the inner magnetosphere externally driven by the solar wind modulation. The second campaign in April was performed by Chandra X-ray Observatory (CXO), XMM newton, and Suzaku satellite simultaneously with Hisaki. Relativistic auroral accelerations in the polar region and hot plasma in the inner magnetosphere were captured by the X-ray space telescopes simultaneously with EUV monitoring of aurora and plasma torus. Auroral intensity in EUV indicated a clear periodicity of 45 minutes whereas the periodicity was not evident in X-ray intensity although previous observations by CXO indicated clear 40-minute periodicity in the polar cap X-ray aurora. In this presentation, we show remarkable scientific results obtained these campaigns. [less ▲]

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See detailBelowground Chemical Ecology: The Case of Wireworms
Barsics, Fanny ULg; Delory, Benjamin ULg; Delaplace, Pierre ULg et al

Conference (2014, December 13)

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See detailMarty and the 4th Logical Investigation: What is the disagreement really about?
Seron, Denis ULg

Conference (2014, December 13)

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See detailBelgien- und Deutschlandbilder als Spiegel der ostbelgischen Nachkriegsgesellschaft
Brüll, Christoph ULg

Conference (2014, December 13)

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See detailTuta absoluta (Lepidoptera: Gelechiidae) ability to localize and develop on wild and cultivated solanaceous plant species
Bawin, Thomas ULg; Dujeu, David; Fagan, Maud ULg et al

Conference (2014, December 13)

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a widespread invasive species damaging economically important cultivated solanaceous crop plants, including tomatoes and potatoes. Little ... [more ▼]

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a widespread invasive species damaging economically important cultivated solanaceous crop plants, including tomatoes and potatoes. Little is known about the ability of this microlepidoptera to encounter and develop on alternative wild and agricultural plant species. These plants could provide refuges and have to be identified for more efficient integrated management strategies. In the present study, we assessed the ability of T. absoluta to develop on wild (Solanum nigrum, Atropa belladonna, Datura stramonium) and cultivated (Solanum tuberosum) solanaceous plant species under laboratory conditions. Fitness tests were performed in Petri dishes by isolating single individuals with excised leaf from one of the host plants. We found that Solanum species allowed higher larval survivability and shorter development time (from egg to adult emergency) compared to the other plants. Two choice behavioral assays performed in flying tunnels (S. tuberosum versus another plant) revealed that adult distribution and female oviposition did not differ between Solanum species, which were preferred to the other tested plants. These results appeared to be consistent with survival rates and development times. Because larval survivability depends on the female’s oviposition choice, the hypothesis that host plant choice is influenced by plant volatile organic compounds has to be tested. It can be concluded that Solanum species remain the more suitable hosts for T. absoluta development among the tested plants. Other plant species could be opportunistically colonized with little incidence but care should be taken in these results as genetic variability in insects and plants, as well as plant physiological state, might have an impact on the pest survivabilty. [less ▲]

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See detailSymptômes neurologiques du bord latéral du pied et de la cheville
WANG, François-Charles ULg

Conference (2014, December 12)

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See detailOpen Access, dream or reality for scholars: The ULg experience
Thirion, Paul ULg

Conference (2014, December 12)

Since the “serial price crisis”, no institution in the world has been able anymore to provide to scholars with the scientific literature they need. Although it was the first reason why the scientific ... [more ▼]

Since the “serial price crisis”, no institution in the world has been able anymore to provide to scholars with the scientific literature they need. Although it was the first reason why the scientific community developed Open Access initiatives, it’s absolutely not the only one. Nowadays, the awareness has become so large that many research players have put OA on the agenda (NIH, WHO, H2020, Wellcome Trust, FRS-FNRS…). The two main existing complementary ways will be described (Green and Gold) and Fools’ Gold will be denounced (unfair gold, hybrid journals, predatory journals). The University of Liege is one of the world leaders in OA with Green and Gold initiatives. What benefits can scholars expect from OA as authors and as readers on the basis of that experience? [less ▲]

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See detailNegations in Late Egyptian: the general trends. A functional and typological perspective
Winand, Jean ULg

Conference (2014, December 12)

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See detailAnalphabétisme secondaire, raison cynique et la culture pour tous.
Hamers, Jérémy ULg

Conference (2014, December 12)

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See detailMaintaining the coexistence of forest species of different shade tolerances with close-to-nature forestry
Ligot, Gauthier ULg; Philippe, Balandier

Conference (2014, December 12)

Continuous-cover forestry has the goal of relying on natural regeneration, and maintaining irregular stand structure and tree species mixture. However, maintaining mixture of species with different shade ... [more ▼]

Continuous-cover forestry has the goal of relying on natural regeneration, and maintaining irregular stand structure and tree species mixture. However, maintaining mixture of species with different shade tolerances appears arduous with such a silvicultural system. Successfully managing irregular and mixed forests, relying on natural processes, requires a strong knowledge of the ecology of natural regeneration. In theory, regeneration dynamics depends upon the amount of transmitted radiation: shade-tolerant species dominate the regeneration under closed canopies whereas less shade-tolerant species dominate the regeneration under partially open canopies. Nevertheless, it explains hardly the difficulties faced by forest managers to maintain the coexistence of species of different shade tolerances. The purpose of this research is to define light requirements of two species with contrasting shade tolerances (Fagus sylvatica L. and Quercus petraea (Matt.) Liebl.) and, next, determining how understory light can be controlled by forest managers. We find that, contrary to our expectations, the shade-tolerant species outgrow the less shade-tolerant species in all light conditions. We next examine the effects of canopy structure and composition on understory light availability with a model of light interception by heterogeneous canopies. Various silvicultural treatments are tested in order to provide favorable understory light conditions for natural regeneration. The results underline that creating favorable understory light conditions for natural regeneration can be achieved with various regeneration treatments. However, the adequate reduction of stand density depends upon the chosen silvicultural strategies. The outcomes of this study highlight that the control of understory light can be insufficient to maintain the coexistence of species of contrasting shade tolerance, and provide guidelines for the management of understory light in heterogeneous forests. [less ▲]

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See detailThe cerato-mandibular ligament: an innovation for sound production and feeding in damselfishes (Pomacentridae).
Olivier, Damien ULg; Frédérich, Bruno; Spanopoulos-Zarco, Milton et al

Conference (2014, December 12)

The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The Pomacentridae ... [more ▼]

The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The Pomacentridae possess a synapomorphic trait, the cerato-mandibular (c-md) ligament, which joins the hyoid bar to the inner part of the lower jaw. It has previously been shown that this ligament is a key trait in communication in damselfishes because it enables them to slam the oral jaws shut causing teeth collision and sound production. This specific behavior of mouth closing could, however, also be used for other tasks, such as feeding. Many territorial damselfishes are referred to as farmers, due to their ability to manage algal crops on which they feed. This study hypothesizes that the c-md ligament provides an advantage for grazing filamentous algae, and should thus be considered a key trait for farming behavior. The kinematic patterns associated with sound production and biting filamentous algae or attached animal prey are all based on the same mechanism and are associated with a slam of the oral jaws. We observed that transection of the c-md ligaments makes the fish unable to perform such actions. This study shows that the c-md ligament is a key trait both for sound production and for grazing activities in damselfishes. The buccal jaw slam enables the fish to perform accurate strikes on small filamentous algae. This kind of bite probably plays a major role in farming activity and allows grazing damselfishes to occupy distinct niches, possibly increasing their competitive evolutionary success. [less ▲]

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See detailSound production in piranhas and relatives: preliminary results
Melotte, Geoffrey ULg; Michel, Christian ULg; Parmentier, Eric ULg

Poster (2014, December 12)

Acoustic communication plays an important role in the life of many teleost species where it is mainly involved in agonistic and/or courtship behaviour(s). Despite the large number of species in the family ... [more ▼]

Acoustic communication plays an important role in the life of many teleost species where it is mainly involved in agonistic and/or courtship behaviour(s). Despite the large number of species in the family Serrasalmidae (92 species), sound production has been described only in some of them, particularly in the genera Serrasalmus and Pygocentrus. The aim of this study is to investigate the sound producing abilities of different Serrasalmidae species and to understand the corresponding mechanisms. Two herbivorous species of Serrasalmidae, Piaractus brachypomus and Metynnis lippincottianus, produce sounds composed of a single pulse. The mechanism involved in these species is not yet understood. In contrast, the calls emitted by several species of Serrasalmus and Pygocentrus are harmonic sounds composed of several pulses without inter-pulse interval. Their mechanism results from the forced vibration of the swimbladder following the contraction of sonic muscles that are attached to tendons surrounding ventrally the bladder. Another species, Pygopristis denticulata, is able to produce two types of sounds. The first sound consists of several pulses with irregular pulse period and is likely produced by a sonic muscle inserting on the skull and on the rostral part of the swimbladder. The second sound is multi-pulsed and, contrary to all other sounds described here, possesses a high dominant frequency suggesting a mechanism that does not involve the swimbladder and that remains to be determined. According to these results, Serrasalmidae seems to contain many species able to produce sounds by means of different mechanisms. [less ▲]

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See detailA morphospace for the marine angelfishes (Pomacanthidae): patterns of diversity
Frederich, Bruno ULg

Poster (2014, December 12)

The Pomacanthidae (marine angelfishes) is an iconic reef fish family of about 88 species. They have a circum-global distribution on tropical to warm-temperate reefs. Marine angelfishes occupy a diverse ... [more ▼]

The Pomacanthidae (marine angelfishes) is an iconic reef fish family of about 88 species. They have a circum-global distribution on tropical to warm-temperate reefs. Marine angelfishes occupy a diverse range of trophic niches, ranging from spongivory and algivory to zooplanktivory. Previous morpho-functional analysis of this family mainly focused on the head region and gut morphology in a limited number of species. However a morphological study including a large sample of angelfish representatives is currently lacking. Here, I explore the diversity of body morphology in this reef fish group. I collected x-ray images of 228 museum specimens from 71 species and I used landmark-based geometric morphometrics to quantify the overall body shape variation within this family. In a test for interspecific allometry, the linear regression of shape variables onto size (LogTL; TL = total length) was significant (P < 0.001). However, the percentage of explained variance in this model (23%) showed no strong relation between size and body shape. Main shape variation across species was explored using a principal component analysis on shape variables. The two main axes (PC1 and PC2) explained 74% of the total variance of the dataset. Pomacanthus spp., which mainly feed on fixed invertebrates (sponges and tunicates), have deep body with high and angular cephalic profile (lowest values along PC1). Conversely, the zooplanktivorous Genicanthus spp., those have a more pelagic lifestyle, show a slender body with shorter anal fin (highest values along PC1). The pigmy angelfishes from the genus Centropyge, which group different diets (omnivory or algivory), show a more robust and rectangular body shape (high scores along PC2). This work provides the row data for future studies dealing with the mode of phenotypic diversification of Pomacanthidae during evolution. [less ▲]

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See detailWhen microplastic is not microplastic: ingestion of artificial cellulose fibers by macrofauna living in seagrass macrophytodetritus
Collard, France ULg; Remy, François ULg; Gilbert, Bernard ULg et al

Poster (2014, December 12)

Vagile macroinvertebrates associated with Posidonia oceanica exported litter were sampled in August 2011, November 2011 and March 2012 in the Calvi Bay (Corsica), near the STARESO oceanographic station ... [more ▼]

Vagile macroinvertebrates associated with Posidonia oceanica exported litter were sampled in August 2011, November 2011 and March 2012 in the Calvi Bay (Corsica), near the STARESO oceanographic station. Contents of digestive tracts were analyzed and fibers of various sizes and colors were found. Fibers were found in 27.6% of the digestive tracts in the nine dominant species. No correlation was found between number of fibers and taxonomic or trophic level. There were no seasonal or spatial preferences and thus we hypothesize that the organisms ingest these fibers randomly throughout the year. Analyses performed with a Raman spectroscope showed that these fibers were composed of cellulose associated with a coloring agent following the fiber color. Red fibers were dyed with the Direct Red 28, blue fibers were dyed with Direct Blue 22. Analyses by a scanning electron microscope (SEM) showed that cellulose fibers had the particular morphology of artificial cellulose fibers called: viscose. Our SEM analyses were compared to literature. This comparison assessed that fibers found in digestive tracts were made of viscose. In a first approach, viscose fibers looked like microplastic fibers because of their color and shape. However, it appeared that these fibers were made of artificial cellulose which is very different than plastic in terms of impacts and fate in the organisms. This study highlights the importance of physico-chemical analyses such as Raman spectroscopy and SEM to certainly identify the composition of particles ingested by organisms. From an ecological point of view, the red coloring agent is known to be carcinogenic in mammals and fish. Consequently, this pollution could provoke an environmental problem for the P. oceanica litter vagile macrofauna. [less ▲]

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See detailStable isotope ratios reveal trophic niche partitioning among hermit crabs from tropical polyspecific seagrass meadows
Michel, Loïc ULg; Frederich, Bruno ULg; Lavitra, Thierry et al

Poster (2014, December 12)

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local ... [more ▼]

Polyspecific seagrass meadows are ubiquitous features of tropical coastal zones. These ecosystems are of critical ecological importance, and provide a wide range of socio-economical services to local populations. Meadows however undergo multiple threats linked to human activities (increased nutrient input, overfishing, invertebrate overharvesting, etc.). It is currently hard to assess how seagrass meadows could respond to anthropogenic impacts due to poor knowledge of their functional ecology. In an effort to unravel trophic interactions ruling the food webs associated to seagrass beds of the Toliara Great Reef (SW Madagascar), we studied resource segregation between two common Diogenidae hermit crabs (Dardanus scutellatus and Ciliopagurus tricolor) using stable isotope ratios. Interspecific differences were noted in isotopic composition of carbon (δ13C = -12.22 ± 1.73 ‰ for D. scutellatus, δ13C = -14.55 ± 0.73 ‰ for C. tricolor), nitrogen (δ15N = 4.73 ± 0.53 ‰ for D. scutellatus, δ15N = 5.20 ± 0.61 ‰ for C. tricolor) and sulfur (δ34S = 14.08 ± 2.32 ‰ for D. scutellatus, δ34S = 16.73 ± 1.49 ‰ for C. tricolor), suggesting that the two species do not feed on the same items. In addition, SIBER (Stable Isotope Bayesian Ellipses in R) modeling based on C and N data clearly showed that no overlap was present in the core isotopic niches of the two species. It also indicated that the isotopic niche of D. scutellatus was greater than the one of C. tricolor, implying that the former feeds on a greater number of items than the latter. While hermit crabs are generally considered as omnivorous species, this study highlighted differences in the foraging ecology of D. scutellatus and C. tricolor. These differences could help to limit competition for food between these two species, and facilitate their coexistence in Malagasy seagrass beds. [less ▲]

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See detailLaïcité à la française : de la prétention universaliste à l'obsession identitaire
Jacquemain, Marc ULg

Conference (2014, December 11)

Le mot "laïcité" est revendiqué par des groupes d'extrême-droite ouvertement racistes et conspirationnistes comme "Riposte laïque". L'article montre que le même imaginaire conspirationniste, sous la même ... [more ▼]

Le mot "laïcité" est revendiqué par des groupes d'extrême-droite ouvertement racistes et conspirationnistes comme "Riposte laïque". L'article montre que le même imaginaire conspirationniste, sous la même structure narrative peut être retrouvé sous des formes débarrassée de leur racisme explicite dans des textes considérés comme "mainstream" au sein de la laïcité, en analysant un texte du Rappel. A partir de là, on tente de montrer que cette "dérivation conspirationniste" peut trouver des prises dans des formulations "progressistes" de la laîcité dès lors que ces formulations partent pour point de départ le point de vue à la fois holiste et idéaliste d'un "laos" toujours-dejà-là, comme chez Peña ruiz [less ▲]

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See detailSur quelques conditions médiatiques de la posture néo-réactionnaire
Durand, Pascal ULg

Conference (2014, December 10)

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See detailIntegrating phylogenetic and environmental niche models to explore speciation mechanisms in the Erythrophleum genus in tropical Africa
Gorel, Anaïs ULg; Fayolle, Adeline ULg; Doucet, Jean-Louis ULg et al

Conference (2014, December 10)

In the context of global climate change, it is of primary importance to understand the species response to climate (habitat tracking or adaptation). In this study we investigated the evolutionary history ... [more ▼]

In the context of global climate change, it is of primary importance to understand the species response to climate (habitat tracking or adaptation). In this study we investigated the evolutionary history of the climatic niche between and within closely related tropical tree species of the Erythrophleum genus (Fabaceae, Caesalpinioideae): E. ivorense, E. suaveolens and E. africanum. Two major hypotheses, the refuge theory and the ecological gradient hypothesis, have been developed to explain the current distribution of tree clades across tropical Africa. To identify the speciation mechanisms, we used a combination of geographic data and environmental factors to quantify the degree of niche conservatism (or divergence). We used two sets of distribution data for the purpose of this study. Species distribution data for the whole of tropical Africa were gathered from herbarium records. Distribution data of the two sister species E. ivorense and E. suaveolens assigned to genetic cluster were available for the lowland tropical forests of western and central Africa. Using a Species Distribution Model (SDM) approach based on MaxEnt algorithm, we tested for the environmental differences (BIOCLIM data) between species and genetic clusters within species. We developed SDMs for each of the three Erythrophleum species (over the whole range) and for each of the five genetic clusters. We quantified the niche overlap using new niche similarity metrics. At species level, the climatic niches differed significantly and overlapped only sligthly, suggesting a parapatric speciation along a climatic gradient. Within the two sister species, the niche of the parapatric central African clusters strongly overlapped, suggesting a secondary contact following the recolonization from different forest refugia. The west African cluster however showed contrasted climatic niches possibly due to either recent (< 100 yrs) climate change in west Africa, or ongoing differentiation on the dry part of the climatic gradient. [less ▲]

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See detailBias-variance decomposition in Random Forests
Louppe, Gilles ULg

Conference (2014, December 09)

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See detailIdentifying the best technical trading rule: a .632 bootstrap approach.
Hambuckers, julien ULg; Heuchenne, Cédric ULg

Conference (2014, December 07)

In this paper, we estimate the out-of-sample predictive ability of a set of trading rules. Usually, this ability is estimated using a rolling-window sample-splitting scheme, true out-of-sample data being ... [more ▼]

In this paper, we estimate the out-of-sample predictive ability of a set of trading rules. Usually, this ability is estimated using a rolling-window sample-splitting scheme, true out-of-sample data being rarely available. We argue that this method makes a poor use of the available information and creates data mining possibilities. Instead, we introduce an alternative bootstrap approach, based on the .632 bootstrap principle. This method enables to build in-sample and out-of-sample bootstrap data sets that do not overlap and exhibit the same time dependencies. We illustrate our methodology on IBM and Microsoft daily stock prices, where we compare 11 trading rules specifications. For the data sets considered, two different filter rule specifications have the highest out-of-sample mean excess returns. However, all tested rules cannot beat a simple buy-and-hold strategy when trading at a daily frequency. [less ▲]

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See detailEvidence based medicine - Tendon and platelet-rich plasma (PRP)
Kaux, Jean-François ULg

in Annual Congress RSBPRM (2014, December 06)

Platelets have known roles in coagulation, inflammatory processes, and immunity modulation; they also have ‘‘restorative’’ properties. Indeed, during degranulation, platelets release different cytokines ... [more ▼]

Platelets have known roles in coagulation, inflammatory processes, and immunity modulation; they also have ‘‘restorative’’ properties. Indeed, during degranulation, platelets release different cytokines and growth factors (VEGF, PDGF, TGF-B, IGF-I, and HGF) that promote angiogenesis, tissue remodeling (bone, skin, muscle, tendon, etc.), and wound healing. PRP is obtained by centrifuging autologous blood to obtain a concentration of platelets, usually between 3 and 10 times that of whole blood, depending on the isolation method. For this reason, different PRP preparation techniques cannot provide a consistently identical final product, but there is currently no international consensus on this issue. Overall, PRP could be an attractive therapeutic option for treating chronic musculoskeletal conditions, such as tendinopathy or plantar fasciitis. Tendons do not have a high metabolic index. Growth factors released by platelets promote tenocyte proliferation, stimulate angiogenesis and have analgesic properties. Thus it could stimulate and accelerate tissue regeneration in animal models. In addition to PRP, optimal tissue quality requires the application of mechanical loads. PRP should be considered for chronic tendinopathies. Indeed, the goal is to initiate an acute inflammatory reaction that quickly moves on to the proliferative phase that involves collagen synthesis. The latter is necessary for appropriate tendon healing. PRP should therefore not be used for acute tendinitis or tenosynovitis. Even if most of the preclinical studies showed that PRP stimulates the tendon healing process, clinical series remain more controversial. Based on literature and our clinical experience, we suggest some ideas for improving this treatment. Optimization of the technique for collecting the PRP is paramount. Different risk factors must be corrected before infiltration, and chronic tendinopathies must be carefully selected. Finally, post-infiltration rehabilitation remains absolutely necessary. Standardisation of the use of PRP remains necessary in order to optimise the results. [less ▲]

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See detailL'Aurignacien de la grotte Yafteh et son contexte
Otte, Marcel ULg; Flas, Damien ULg; Zwyns, Nicolas et al

Poster (2014, December 06)

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See detailScience at the fringe of the French colonial world: the circulation of Jean-André Peyssonnel’s treatises on medicine and the natural history of Guadeloupe
Vandersmissen, Jan ULg

Conference (2014, December 05)

Jean-André Peyssonnel, born in Marseille in 1694, was a physician who in 1720-1721 earned his spurs in the fight against the plague that ravaged his hometown. Fascinated by the sea and the work of ... [more ▼]

Jean-André Peyssonnel, born in Marseille in 1694, was a physician who in 1720-1721 earned his spurs in the fight against the plague that ravaged his hometown. Fascinated by the sea and the work of fishermen, he turned in following years to the study of marine life. While conducting observational research along the coasts of the Provence and North Africa, he found evidence of the animal nature of coral. This controversial claim caused a heated debate with Réaumur, who blocked his career plans in France. Out of necessity Peyssonnel was forced to accept the post of “Médecin du Roi” in Guadeloupe. In this remote outpost of the French colonial empire, Peyssonnel continued his scientific activities. He received orders to fight a local outburst of leprosy, which resulted in a comprehensive study on the disease. In addition, he produced dozens of treatises on the natural history of the French Antilles, including volcanic, oceanographic, botanical and zoological studies. This contribution tells the story of these remarkable documents which, on their way to the learned world in Europe, passed through many hands. Spread over several intellectual centres – London, Paris, Marseille, Rouen, Bordeaux, Angers – these manuscripts shed a unique light on the circulation of knowledge in a Republic of Sciences turning global. [less ▲]

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See detailL’impact de la réserve cognitive sur le fonctionnement exécutif au cours du vieillissement normal
Simon, Jessica ULg; Gilsoul, Jessica ULg; Collette, Fabienne ULg

Conference (2014, December 05)

Le vieillissement normal s’accompagne d’un dysfonctionnement exécutif important. Or, on sait aujourd’hui qu’il existe une forte variabilité interindividuelle quant aux effets du vieillissement sur la ... [more ▼]

Le vieillissement normal s’accompagne d’un dysfonctionnement exécutif important. Or, on sait aujourd’hui qu’il existe une forte variabilité interindividuelle quant aux effets du vieillissement sur la cognition. Selon l’hypothèse de la réserve cognitive (Stern, 2009), les individus qui auraient développé un haut niveau de réserve résisteraient mieux aux effets du vieillissement que des individus de plus faible réserve cognitive. Dans cette étude, nous avons voulu mesurer l’impact des facteurs de réserve cognitive sur le fonctionnement exécutif au cours du vieillissement normal. Nous avons recruté 59 participants âgés de 60 à 80 ans, sans trouble cognitif ni neurologique. Nous leur avons proposé 8 tâches cognitives évaluant le fonctionnement exécutif : des épreuves d’inhibition (test de Stroop, test de Hayling, subtest Incompatibilité de la TAP), de flexibilité (subtest Flexibilité de la TAP, épreuve d’alternance arithmétique « plus-moins ») et de mise à jour (subtest Lettre-Chiffre de la MEM III, mise à jour de consonnes et tâche de 2-back). De plus, nous leur avons demandé de compléter différents questionnaires évaluant quatre facteurs de réserve cognitive (niveau d’études, parcours professionnel, activité physique et activités de loisir). Nous avons réparti nos participants en deux groupes en fonction de leur niveau de réserve cognitive (faible et haute). Des analyses de t de student (p<0.05) montrent que les participants avec une haute réserve cognitive ont de meilleures performances au subtest de mise à jour des consonnes (p=0,05) ainsi que des résultats quasi significatif pour le score composite de mise à jour (p=0,06) et le subtest de mise de consonnes (p=0,06). Nous avons aussi évalué l’impact spécifique de chaque facteur de réserve cognitive sur les performances au moyen de régressions simples (p<0.05). Les données montrent que le niveau d’étude explique une part significative de la variance du score composite de mise à jour et aux subtests Flexibilité et Lettre-Chiffre ainsi qu’une part quasi significative de la variance au score de mise à jour de consonnes. L’activité professionnelle au cours de la vie explique une part quasi-significative de la variance pour le score de mise à jour (p=0,07) et pour le test de mise à jour de consonnes (p=0,07). Enfin, les activités de loisir, quant à elles, expliquent une part significative de la variance des performances à la tâche du 2-back et une part quasi-significative de la variance des performances au test de Hayling (p=0.06). En conclusion, il apparait que les sujets âgés avec un haut niveau de réserve cognitive montrent de meilleures capacités à certains tests exécutifs uniquement. De plus, nos données suggèrent que tous les aspects du fonctionnement exécutif ne sont pas impactés de façon similaire par les différents facteurs de réserve cognitive. [less ▲]

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See detailCan Electrical Resistivity Tomography offer us a dynamic view on what happens in the soil-plant continuum?
Garré, Sarah ULg

Conference (2014, December 05)

Root water and nutrient uptake and its relation to environmental factors is one of the least understood components in the terrestrial water balance and is of high importance for water resources management ... [more ▼]

Root water and nutrient uptake and its relation to environmental factors is one of the least understood components in the terrestrial water balance and is of high importance for water resources management, ecology and agriculture. As the processes in the soil-plant continuum are complex and inextricably intertwined, alternative, non-invasive measurement methods are necessary to unravel spatial and temporal dynamics of the system. Electrical resistivity tomography (ERT) has been proposed as a promising technique, since bulk resistivity maps and their temporal evolution may serve as a proxy for changes in soil moisture and pore water salinity, amongst others. However, the variables affecting the measured bulk electrical resistivity often change simultaneously in natural environments and not all influencing factors are yet well understood (e.g. influence of root biomass). Therefore, the method needs field-specific calibration. In addition to limitations due to signal-to-noise ratio and data inversion strategies, this implies that ERT still needs further development and research efforts for its use to characterize the soil-plant continuum. [less ▲]

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See detailRegistres de la langue en néo-égyptien: le cas des Tomb Robberies
Winand, Jean ULg

Conference (2014, December 05)

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See detailAdvances in proteomics for the FP7 Venomics project
Degueldre, Michel ULg; Quinton, Loïc ULg; De Pauw, Edwin ULg

Conference (2014, December 03)

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See detailMid-winter freeze experiment in the Arctic Ocean: Norwegian Young sea ICE cruise (N-ICE2015)
Nomura, Daiki; Granskog, Mats A.; Fransson, Agneta et al

Poster (2014, December 02)

In mid-January 2015, RV Lance will freeze into the ice north of Svalbard, Arctic Ocean at around 83.25°N 30°E, and passively drift with the ice as part of the Norwegian Young sea ICE cruise (N-ICE2015 ... [more ▼]

In mid-January 2015, RV Lance will freeze into the ice north of Svalbard, Arctic Ocean at around 83.25°N 30°E, and passively drift with the ice as part of the Norwegian Young sea ICE cruise (N-ICE2015). Judging from historic sea ice drift trajectories, it is likely that RV Lance will drift in a SW direction and the ship will probably be freed from the ice in mid spring after about two to three months of drift. Thereafter, RV Lance will return to her starting position and start a new drift. Under all circumstances, the ice drift project will end in late June 2015. Throughout the cruise the focus will be on the interaction of the atmosphere-ice-ocean system and the response of the marine ecosystem to the thinner ice regime. The overall goal of the project team is to improve our understanding of the role of the younger ice pack in the Arctic on greenhouse gas fluxes and to ultimately assess whether the Arctic Ocean is a sink or source of greenhouse gases. We plan to conduct long-term synchronous observations of Arctic snow and sea ice biogeochemistry and physics and fluxes of carbon dioxide, methane, nitrous oxide and bromoform. This work targets at filling a crucial gap in our understanding of the role of Arctic sea ice in the climate system. This is done by conducting state of the art observations on Arctic sea ice in the polar night, when observations are basically non-existent. Further we are focusing on the new thinner ice regime, which is even less documented. We aim to understand how the thinner sea ice in the Arctic basin contributes (i) to important greenhouse gas exchange between the atmosphere and ocean and (ii) to aerosol formation, that contribute to the radiative balance of the planet. This work will increase direct collaboration between Japanese and European scientists in the Arctic, and combines complimentary expertise and experience from several international partners to carry out the interdisciplinary work proposed. [less ▲]

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See detailDetecting microbial patterns in relation to soil agricultural practices and the plant development stage
Degrune, Florine ULg; Dufrêne, Marc ULg; Taminiau, Bernard ULg et al

Poster (2014, December 02)

Agricultural practices have a strong impact on soil bacteria and fungi. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these ... [more ▼]

Agricultural practices have a strong impact on soil bacteria and fungi. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these effects in relation to changes induced by agriculture and plant stage in soil conditions. Some bacteria are influenced only by the plant stage, which induces changes in soil humidity, pH, nitrates, and carbon. We would thus expect these bacteria to be highly sensitive to these parameters. Other bacteria are affected only by the tillage practice applied. Further study is needed to identify the soil parameters responsible for this effect. The plant stage also has a great impact on fungal community composition. [less ▲]

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See detaill'âgisme
Marquet, Manon ULg; Schroyen, Sarah ULg

Conference (2014, December 01)

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See detailPhysisorbed poly(ethylene oxide) is a robust tether for AFM-based single-molecule force spectroscopy
Willet, Nicolas ULg; Lussis, Perrine ULg; Giamblanco, Nicoletta et al

Conference (2014, December 01)

Atomic force microscopy (AFM)-based single-molecule force spectroscopy is a prevalent tool for the exploration of individual (bio)molecules, providing exquisite information on many molecular-level ... [more ▼]

Atomic force microscopy (AFM)-based single-molecule force spectroscopy is a prevalent tool for the exploration of individual (bio)molecules, providing exquisite information on many molecular-level processes. For example, proteins, DNA, polysaccharides, supramolecular polymers and polyelectrolytes have been investigated, revealing details about the strength of intramolecular interactions, folding and unfolding pathways, mechanics, conformational changes, reactivity, kinetics, etc. For each particular system under investigation, the experimental design is a decisive phase that often involves a multistep chemical protocol, including grafting, derivatization, coupling, (de-)protection, and other functionalization reactions. Procedures of sample preparation are often complex and time-consuming. Hence, there is a need for new general platforms allowing for straightforward sample preparation adapted to single-molecule studies, i.e. a tight attachment to both the substrate and the tip, and a low density to favor single-molecule detection. We report here on the use of poly(ethylene oxide) (PEO) as a tether to probe various properties of individual molecules. The polymeric linker acts as a handle that stably binds to the AFM tip. The simple adsorption of poly(ethylene oxide) to the tip is versatile and provides an appropriate system configuration for the investigation of many different biological and synthetic molecular systems. To attest for this versatility and adequacy with advanced single-molecule investigation, we present different examples of PEO-mediated studies about the unfolding of a synthetic peptide, the mechanochemical behavior of a molecular machine and finally the stability of a metallo-supramolecular complexed polymer. All the requirements for the study of peptide conformation, tiny molecular machines or metallo-supramolecular interactions in solution are here fulfilled. More generally, this method based on non-covalent sorption of PEO on an AFM tip, can be implemented in a wide range of solvents, for the study of many intra- or intermolecular phenomena at the single-molecule level over orders of magnitude of force loading rates. Connecting PEO tethers to a very broad variety of (bio)molecules is a facile and versatile route. The commercial availability of many different functional PEOs makes this tethering strategy even more accessible. [less ▲]

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See detailEcology of 20 trace elements in Mytilus galloprovincialis
Richir, Jonathan ULg; Lepoint, Gilles ULg; Lejeune, Pierre et al

Conference (2014, December)

Trace elements (TEs) are considered as non-degradable pollutants. This persistent character can alter their natural biogeochemical balance in contaminated environments. TEs are further toxic for aquatic ... [more ▼]

Trace elements (TEs) are considered as non-degradable pollutants. This persistent character can alter their natural biogeochemical balance in contaminated environments. TEs are further toxic for aquatic organisms from threshold levels and are thus likely to cause multiple damages to the population, the community and the ecosystem levels. For these reasons, their environmental occurrence has to be accurately monitored. The main interest of the use of quantitative sentinel organisms to this end, or bioindicator species, is their capacity to give information on the bioavailability of environmental contaminants. Mussels from the genus Mytilus are particularly well suited organisms for the monitoring of the coastal contamination. Native wild and cultured Mytilus galloprovincialis Lamarck, 1819 have been widely used since around 40 years to this purpose along coasts of the eastern Atlantic and the Mediterranean. But the accurate use of a bioindicator relies on the detailed knowledge of its ecophysiology and the influence of environmental variables on the bioaccumulation processes. In the framework of the STARECAPMED project, the ecology of 20 TEs in M. galloprovincialis is therefore investigated. The mussel morphometry and biology firstly define the TE accumulation processes. Accumulated TE are internally regulated and redistributed between body compartments; these internal processes notably depend on the essential or non-essential character of TEs. As filter feeder, mussels accumulate soluble and suspended TEs whose environmental levels are determined by the geomorphology, the physico-chemistry and the hydrology of monitored coastal meadows. All these factors are acting together to modulate the TE accumulation processes in mussels. TE bioaccumulated levels further balance quickly when any physiological or environmental changes occur in order to reach a new steady-state with environmental TE loads. The ecology of TEs in M. galloprovincialis is thus complex and very dynamic; these considerations must be taken into account when monitoring the chemical contamination of coastal meadows. [less ▲]

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See detail“Negotiating the law: judges, families and court hearings in inheritance cases in Cotonou”
Andreetta, Sophie ULg

Conference (2014, December)

On 14 June 2004, Benin ratified a new Code on Persons and Family – regulating, amongst other things, inheritance matters. Over the last couple of years, the number of inheritance cases handled by state ... [more ▼]

On 14 June 2004, Benin ratified a new Code on Persons and Family – regulating, amongst other things, inheritance matters. Over the last couple of years, the number of inheritance cases handled by state courts has drastically increased in the capital city. How do people understand and use the law? While modern courts, like other public services, have been analyzed as part of everyday governance and the local state in West Africa, I on the other hand will focus on the ways in which the judiciary interprets and implements law. Within that context, hearings are a key moment of legal proceedings. How do litigants argue their cases? How do judges manage to settle inheritance quarrels? Using case studies of inheritance disputes and their litigation under the 2004 ‘Code on Persons and Family’, I try to understand how state laws are understood, utilized, and/or questioned in Cotonou. [less ▲]

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See detailLes corps des scientifiques et de l’intellectuel dans la revue La recherche
Dondero, Maria Giulia ULg

in Havelange, Carl (Ed.) La lettre et l'image (2014, December)

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See detailEcological diversity of damselfishes (Pomacentridae) from Moorea
Gajdzik, Laura ULg; Parmentier, Eric ULg; Lepoint, Gilles ULg et al

Poster (2014, December)

The Pomacentridae (damselfishes) represents the 3rd most species-rich coral reef fish family, including 394 species spread worldwide. Recent studies suggested that damselfishes iteratively evolve along ... [more ▼]

The Pomacentridae (damselfishes) represents the 3rd most species-rich coral reef fish family, including 394 species spread worldwide. Recent studies suggested that damselfishes iteratively evolve along three main trophic guilds: “the pelagic feeders” feeding mainly on zooplankton, “the benthic feeders” grazing filamentous algae or biting coral polyps, and an “intermediate” group with species feeding on zooplankton, small benthic invertebrates and algae in variable proportions. Comparing the ecological diversity of damselfish communities among various geographical regions could assess such an iterative radiation. Within this context, the present study is a first attempt exploring the trophic diversity of damselfish community at Moorea Island (French Polynesia). The relationship between the trophic guilds, their habitat diversity and their behavior is evaluated. Habitat and behaviour of 16 damselfish species were described during transects along which different kinds of data were collected. For examples, the location within the reef (e.g. fringing or barrier reef), the depth, the substrate over which fishes are living or the solitary/gregarious habits were recorded. Diet of individuals was investigated by means of stomach content and stable isotopes analyses. Correlations between the dietary preferences and ecological data (habitat and behaviour) are given and the ecological diversity among trophic groups is compared. [less ▲]

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See detailQuasi-loss of nationality - policy recommendations
Wautelet, Patrick ULg; de Groot, Gérard-René

Conference (2014, December)

This presentation gives a summary of the policy recommendations which were drafted in the framework of the ILEC project on loss of nationality (http://www.ilecproject.eu/). The recommendations pertain to ... [more ▼]

This presentation gives a summary of the policy recommendations which were drafted in the framework of the ILEC project on loss of nationality (http://www.ilecproject.eu/). The recommendations pertain to the situation of 'quasi-loss', i.e. the situation in which a person who was assumed to possed a nationality, learns that he/she never has possessed that nationality. The recommendations were presented in a policy brief (available at www.ceps.eu/node/9871). They will be included in Guidelines. [less ▲]

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Peer Reviewed
See detailOral Abstract session: Stress echo in clinical practice: Friday 5 December 2014, 08:30-10:00Location: Agora.
Magne, J.; Donal, E.; Dulgheru, R. et al

Conference (2014, December)

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See detailPoster session 5: Friday 5 December 2014, 14:00-18:00Location: Poster area.
Henri, C.; DULGHERU, Raluca Elena ULg; Magne, J. et al

Poster (2014, December)

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See detailTree species diversity effects on soil microbial biomass, diversity and activity across European forest types
Carnol, Monique ULg; Baeten, Lander; Bosman, Bernard ULg et al

Conference (2014, December)

Increasing tree species diversity in forests might contribute to ecosystem-service maintenance, as well as to the reconciliation of regulating, provisioning and supporting services within the frame of ... [more ▼]

Increasing tree species diversity in forests might contribute to ecosystem-service maintenance, as well as to the reconciliation of regulating, provisioning and supporting services within the frame of multifunctional and sustainable forestry. Individual tree species influence biogeochemical cycling through element deposition (throughfall, litterfall), and through microbial activities in the soil. Yet, the influence of mixing tree species on these ecosystem processes is unclear, in particular concerning the microbial diversity and activity in soils. Here we synthesize results from the Exploratory Platform of the FunDivEUROPE project (http://www.fundiveurope.eu/). This network of 209 comparative plots covering a tree diversity gradient of 1 to 5 tree species was established in existing mature forests in 6 European regions. These six focal regions represent a gradient of major European forest types from boreal to Mediterranean forests. We analysed the impact of tree species diversity and the role of other controlling factors on the metabolic diversity of soil bacteria (BIOLOG Ecoplate), soil microbial biomass (fumigation-extraction) and potential nitrification (shaken soil slurry) in the forest floor and the upper organo-mineral soil horizon. Mean values of microbial biomass carbon ranged from 240 (Poland) to 1762 (Germany) mg kg-1 in the forest floor and from 4197 (Italy) to 11207 (Finland) mg kg-1 in the upper organo-mineral horizon. Tree diversity and soil water content were important controlling factors. Statistical models predict microbial biomass to increase in both horizons by 7-8% with each step increase in tree diversity. Metabolic diversity of soil bacteria (% of substrates used) showed high variability both within and between sites. Further results analysed with mixed linear models will be presented and discussed. [less ▲]

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See detailMOTOR CONTROL AND LOW BACK PAIN IN ELITE SOCCER PLAYERS
GROSDENT, Stéphanie ULg; Demoulin, Christophe ULg; Namurois, Guy et al

Poster (2014, December)

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See detailThe influence of bioavailable copper and zinc concentrations on metallothionein levels, DNA damage and gene expression in the polychaete Nereis (Alitta) virens (M. Sars, 1835)
Pini, Jennifer; Richir, Jonathan ULg; Watson, Gordon

Poster (2014, December)

Nereis (Alitta) virens is an ecologically and commercially important polychaete of intertidal soft sediment and an ideal species to investigate long term effects of metals. Using a spike approach, worms ... [more ▼]

Nereis (Alitta) virens is an ecologically and commercially important polychaete of intertidal soft sediment and an ideal species to investigate long term effects of metals. Using a spike approach, worms (1-3 g) were incubated for nine months in sediments spiked at environmentally relevant concentrations of copper, zinc and copper & zinc together: low (copper: 70 mg kg-1, zinc: 200 mg kg-1), medium (copper 120 mg kg-1, zinc: 270 mg kg-1) and high (copper 575 mg kg-1, zinc: 1160 mg kg-1) concentrations. These concentrations were based on an extensive sampling regime of sediment, pore water and worms from seven sites with different levels of contamination across the UK. Worms were fed and maintained under ambient conditions in a flow-through seawater system and sampled at 3, 6 and 9 months. Using BCR sequential extraction, bioavailable metal concentrations in the sediment were assessed in addition to pore water and tissues metal concentrations. The induction of metallothionein (MT) activity, especially at month 6, revealed the detoxification potential of N. virens under metal stress conditions. Significant correlations were obtained between copper bioavailable concentrations in the sediment and MT levels at month 3 and between zinc bioavailable concentrations in the sediment and MT levels at month 6. The highest DNA damage was recorded at month 3 for high copper & zinc combined treatment with 36.44%. Significant correlations were obtained between sediment bioavailable metal concentrations and DNA damage. In addition, the study of metal induced gene expression will reveal for the first time metal regulation process in the polychaete N. virens. This study showed that (1) not only high copper was toxic to N. virens but high copper & zinc combined treatment was the most toxic to the worms and (2) MT and DNA damage were sensitive and reliable endpoints used to evaluate copper and zinc toxicity. [less ▲]

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See detailClub 35 Poster session 2: Thursday 4 December 2014, 08:30-18:00Location: Poster area.
Voilliot, D.; Magne, Jm; DULGHERU, Raluca Elena ULg et al

Poster (2014, December)

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See detailPoster session 4: Friday 5 December 2014, 08:30-12:30Location: Poster area.
Uejima, T.; Itatani, K.; Nakatani, S. et al

Poster (2014, December)

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See detailTrace element kinetics in caged Mytilus galloprovincialis
Richir, Jonathan ULg; Lepoint, Gilles ULg; Donnay, Annick et al

Poster (2014, December)

Trace elements (TEs) remain contaminants of concern because of their persistence, ability to concentrate in organisms and toxicity. The Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 is a ... [more ▼]

Trace elements (TEs) remain contaminants of concern because of their persistence, ability to concentrate in organisms and toxicity. The Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 is a relevant bioindicator of TE coastal contamination. However, little research has studied the combined influence of environmental condition changes and physiological processes on their kinetics in that species. Caged M. galloprovincialis were thus immerged in 2 contrasted pristine Corsican (France) coastal environments, the semi-enclosed Diane salty pond and the open Calvi Bay, from February to June 2011. Mussels were regularly sampled to study the kinetics of 19 TEs in their flesh; dissolved and particulate TEs were also monitored. The primary production and the water physico-chemical variables were measured, and meteorological data were purchased from Météo-France. TE kinetics in mussels differed between sites. Mussel spawning, a temperature and saline-induced physiological process that occurred about 10 days later in the Diane pond, was followed by a short time increase of TE levels in the mussel flesh. Mussel contamination also evolved according to changes of their respective environmental TE levels. Raining events temporary led, in the Diane pond, to the water enrichment with TEs, nutrients and detrital material, to peaks of primary production and to the increase of TE concentrations in the mussel flesh. This step by step evolution of TE levels in the environment and mussels was afterwards followed by a rapid return to initial conditions. In the open Calvi Bay, these fast and balanced kinetics were not so obvious, because of the rapid dilution of environmental constrain effects in the Bay. Mussels are often used as bioindicator in estuaries and coastal enclosed meadows with rapidly changing environmental conditions. In such conditions, the influence of the environment on TE kinetics in mussels must be considered, in addition to physiological processes, when monitoring the TE coastal contamination. [less ▲]

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See detailPoster session 3: Thursday 4 December 2014, 14:00-18:00Location: Poster area.
Gabriels, C.; LANCELLOTTI, Patrizio ULg; Van De Bruaene, A. et al

Poster (2014, December)

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See detailEvaluation of pairwise calibration techniques for range cameras and their ability to detect a misalignment
Lejeune, Antoine ULg; Grogna, David ULg; Van Droogenbroeck, Marc ULg et al

in International Conference on 3D Imaging (IC3D) (2014, December)

Many applications require the use of multiple cameras to cover a larger volume. In this paper, we evaluate several pair-wise calibration techniques dedicated to range cameras. We compare the precision of ... [more ▼]

Many applications require the use of multiple cameras to cover a larger volume. In this paper, we evaluate several pair-wise calibration techniques dedicated to range cameras. We compare the precision of a self-calibration technique based on the movement in front of the cameras to object based calibration. While the self-calibration method is less precise than its counterparts, it yields a first estimation of the transformation between the cameras and permits to detect when the cameras become mis-aligned. Therefore it is useful in a practical situations. [less ▲]

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See detailWood digestion in lower termites: multidisciplinary approaches based on differential feeding
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, December)

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical ... [more ▼]

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical environments found in lower termites paunch are potentially the key point to explain the efficiency of ligno-cellulose digestion. Contribution of advancing technologies accelerates the progress of our knowledge in this field. Here, we present multiple approaches combining old and recent techniques used to highlight the effect of ligno-cellulosic compounds on termite gut and the role of populations from the symbiotic microbial community. Termites Reticulitermes flavipes (Kollar) submitted to various artificial diets showed variations in flagellates populations profile and enzymatic activities. Differential protein expression was investigated using 2D-DIGE MALDI-TOF-TOF and 2D-LC-MS/MS using high resolution orbitrap analyzer. Results from both proteomic experiments tend to support each-other and bring complementary points of view. The gel-free analysis resulted in highly contrasted identification of enzymes involved in ligno-cellulose digestion and metabolism. Finally, differential feeding experiments leaded to in vivo selection of different symbiotic communities. These communities were characterized following some metabolism assays and allowed the cultivation of diverse microbial consortia using media closely related to the respective artificial diets. This work provides relevant data on termite and associated microbial community response to alimentary diets. [less ▲]

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See detailImpact of shading on meiofauna in a Posidonia oceanica meadow
Pete, Dorothée ULg; Gobert, Sylvie ULg

Poster (2014, December)

Posidonia oceanica meadow is an endemic ecosystem of the Mediterranean coasts. A known threat to this ecosystem is aquaculture. In zones of intensive fish production, P. oceanica meadow tends to be less ... [more ▼]

Posidonia oceanica meadow is an endemic ecosystem of the Mediterranean coasts. A known threat to this ecosystem is aquaculture. In zones of intensive fish production, P. oceanica meadow tends to be less healthy or to disappear .One of the reasons for this is a decrease in the light that reaches the leaves (direct shading, increase of water turbidity or of epiphytic algae density). Unfortunately, when the meadow begins to die, it is often too late to act. So, people are trying to find indicators that react early to this kind of perturbations. In this framework, this study focuses on the impact of shading (without nutrient enrichment) on the meiofauna living in the surface sediment of a P. oceanica meadow. An in situ shading experiment was led from the end of May to the end of August 2009, at a depth of 10 m, in a reference P. oceanica meadow. Three shading nets were put in the meadow to reach a light extinction of 50%. A control site was also defined. The first two centimetres of sampled sediment cores were studied. After three months of shading, the total abundance of meiofauna at the shading site was lower than at the beginning of the experiment, while it stayed around the same level at the control site. This difference is mainly due to a decrease in the total number of foraminiferans, nematods, gnathostomulids, copepods and bivalves. However, no significant difference in diversity was observed. At the end of this experiment, it appeared that, contrarily to what is mostly said in the literature, the direct organic enrichment that occurs at fish farms is not the only reason to the modification of the meiofauna communities of the ecosystem. The shading by itself has also an effect. [less ▲]

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See detailPoster session 1: Wednesday 3 December 2014, 09:00-16:00Location: Poster area.
Romano, G.; D'ancona, G.; Pilato, G. et al

Poster (2014, December)

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See detailEvidence of a fine-scale genetic structure for the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees
Gillet, François ULg; Cabria Garrido, Maria Teresa; Blanc, Frédéric et al

Poster (2014, December)

The Pyrenean desman (Galemys pyrenaicus) is a small semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in mountain streams of cold and well ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is a small semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in mountain streams of cold and well-oxygenated flowing waters (Nores et al. 2007). This species is currently considered as vulnerable in the IUCN Red List (Fernandes et al. 2008) and has been suffering from habitat loss and fragmentation for decades, inevitably impacting its distribution. The ecology and biology of this species are poorly known, notably because of its elusive behavior and its primarily nocturnal activity (Stone 1987, Bertrand 1994). Its distribution area is even not definitively established. Furthermore, a recent genetic study, based on mitochondrial and intronic sequences (Igea et al. 2013), showed that the genetic variability of the Pyrenean desman is very low in the Pyrenees. In this study we investigated the potential existence of a genetic structure and gene flow at a smaller scale using 24 polymorphic microsatellites loci. As the Pyrenean desman is a very elusive species, we completed our sample collection of tissues with faeces samples coming from the French distribution area of this species. Doing so, we successfully identify 70 individuals out of 355 faeces samples. Bayesian analyses revealed a cryptic genetic structure in our data set. Three clusters were evidenced (one western, one central and one eastern) and gene flow appears to be limited between these clusters (min. Fst value of 0.2). [less ▲]

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See detailA physically motivated pixel-based model for background subtraction in 3D images
Braham, Marc ULg; Lejeune, Antoine ULg; Van Droogenbroeck, Marc ULg

in International Conference on 3D Imaging (IC3D) (2014, December)

This paper proposes a new pixel-based background subtraction technique, applicable to range images, to detect motion. Our method exploits the physical meaning of depth information, which leads to an ... [more ▼]

This paper proposes a new pixel-based background subtraction technique, applicable to range images, to detect motion. Our method exploits the physical meaning of depth information, which leads to an improved background/foreground segmentation and the instantaneous suppression of ghosts that would appear on color images. In particular, our technique considers certain characteristics of depth measurements, such as failures for certain pixels or the non-uniformity of the spatial distribution of noise in range images, to build an improved pixel-based background model. Experiments show that incorporating specificities related to depth measurements allows us to propose a method whose performance is increased with respect to other state-of-the-art methods. [less ▲]

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See detailEffect of tree species mixture on earthworm communities on a continental scale
De Wandeler, Hans; Baeten, Lander; Carnol, Monique ULg et al

Poster (2014, December)

The belowground food web represents a major part of associated biodiversity in forest ecosystems, and plays a significant role in the ecosystem processes of litter decomposition and nutrient turnover ... [more ▼]

The belowground food web represents a major part of associated biodiversity in forest ecosystems, and plays a significant role in the ecosystem processes of litter decomposition and nutrient turnover. Past research has demonstrated overwhelming evidence of strong tree species identity effects on earthworm communities. It has been proposed that increased plant community diversity would be beneficial to the abundance and diversity of the belowground food web, but effects of tree species diversity on earthworm communities have seldom been reported, and are inconclusive. In this study at continental scale we evaluated whether tree species diversity positively affects earthworm biomass and diversity. For this purpose the FunDivEUROPE Exploratory Platform was used with 209 plots in 6 regions well spread over Europe with a low within-region site variability, but a within-region tree species diversity gradient from monocultures to 3 or 4 species plots. In every plot earthworms were sampled using a combined method of mustard extraction and hand sorting of litter and a soil monolith. Data are being analysed with multivariate tools and mixed effects models. First results suggest only limited influence of tree diversity on the biomass of earthworm communities at continental scale. Tree diversity effects are weak, context specific and interacting with tree identity. In nutrient poor soils we found a negative tree diversity effect on earthworm biomass when deciduous monocultures are enriched with coniferous species, while in rich soils we found a positive tree diversity effect which could be related with the food security this provides to the earthworm community. [less ▲]

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See detailComment finir, ou la prière faite au théâtre
Dubouclez, Olivier ULg

in Littérature (2014, December)

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See detailPoster session 6: Saturday 6 December 2014, 08:30-12:30Location: Poster area.
Henri, C.; DULGHERU, Raluca Elena ULg; Magne, J. et al

Poster (2014, December)

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See detailStigmergy as a mechanism to produce collective vortex behaviours: a study case in shoveler duck
Delcourt, Johann ULg; bode, W. Nikolaï

Poster (2014, December)

Ant mill, caterpillar circle, bat donut, bacteria vortex, duck swirl and fish torus are different names for rotating circular formations of animals, where individuals turn around a common centre. Even if ... [more ▼]

Ant mill, caterpillar circle, bat donut, bacteria vortex, duck swirl and fish torus are different names for rotating circular formations of animals, where individuals turn around a common centre. Even if the ubiquity of this behavioural phenomenon might have suggested common causes or fundamental underlying principles across contexts, a variety of proximate mechanisms can give rise to vortex behaviours. Here, we investigate if stigmergic process (mechanism of self-organisation wit hout direct communication or interaction between individuals) is able to produce different collective behaviours, notably collective vortices. We present an individual-based simulation model for the movement of populations in a resource landscape that allows us to vary the strength of the interactions mentioned above. The key assumption and novelty of our model is that individuals can cause the release of additional nutrients, as well as consuming them. Our model produces clear predictions. For example, we expect more tortuous individual movement paths and higher levels of aggregation in populations occupying homogeneous environments where individual movement makes more nutrients available. We also show how observed movement dynamics could change when local nutrient sources are depleted or when the population density increases. Our predictions are testable and qualitatively reproduce the different feeding behaviours observed in filter-feeding ducks (Anas clypeata), for example. We suggest that considering two-way interactions between feeding individuals and resource landscapes could help to explain fine-scale movement dynamics. [less ▲]

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See detailAthanase Kircher ou l’Oedipe des Temps modernes
Winand, Jean ULg

Conference (2014, November 29)

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See detailPerceptions culturelles dans un contexte de néoplasme pédiatrique: Considérations transculturelles entre l’Occident et le Japon
Vander Haegen, Marie ULg; Roskam, Isabelle

Poster (2014, November 29)

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et ... [more ▼]

INTRODUCTION Toute société humaine construit son héritage collectif sur la transmission de valeurs et de rites permettant aux descendants de réguler leurs schèmes comportementaux, affectifs, sociaux et moraux sur base des repères culturels alloués depuis des décennies. Investiguer les balises culturelles et l’appréhension sociétale du néoplasme pédiatrique reste peu développé dans la littérature. Ce champ « anthropo-clinique » prend initialement son essor dans la clinique oncologique adulte où des résultats significatifs montrent un lien entre la culture et la perception du cancer. OBJECTIFS Les objectifs de cette étude sont multiples: (a) étudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant, (b) analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale, et (c) mettre en exergue les diverses implications cliniques pour les professionnels de la santé. METHODE Une revue de littérature est conduite afin de dégager les tendances culturelles dans la politique sanitaire, le service hospitalier, la relation médecin-malade, le concept de douleur, l’environnement familial et la perception de la mort au Japon et en Occident. Le choix s’est porté sur ce pays asiatique par la présence de certains repères culturels intéressants dans le domaine de l’oncologie pédiatrique. RESULTATS (a) Etudier l’effet de la culture dans la représentation de la maladie cancéreuse de l’enfant Les correspondances effectuées ont permis d’entrevoir la situation onco-pédiatrique comme relevant de faits biologiques et de faits culturels. Les paramètres culturels interviennent au sein des nombreuses conceptions et représentations sociales de la maladie cancéreuse d’un enfant. L’ensemble de ces perceptions sociales étant notamment influencées par les valeurs et les truismes véhiculés dans la culture. (b) Analyser transculturellement les similitudes et les dissimilitudes entre la culture japonaise et occidentale. Eléments de similitudes : Il a été noté l’universalité des mécanismes pathogéniques du cancer chez l’enfant et le développement de politiques sanitaires dans la lutte contre le cancer. Eléments de dissimilitudes : A. Le Japon Le système familial japonais et son organisation des soins en cancérologie de l’enfant varient et se teintent de nombreuses coutumes shintoïstes et bouddhistes. Il a été identifié le chaos régnant dans ses familles où des attitudes désengagées existent. L’organisation hospitalière japonaise est décrite comme carencée en réseaux de soutien psychologique pour la mère, très souvent seule face à la maladie de son enfant. Des risques majeurs de dépression, de traits anxieux et d’un stress post-traumatique sont observés. Le médecin japonais dispose d’un pouvoir de décision médicale et sociale, se manifestant notamment par un degré de variabilité dans la communication diagnostique et pronostique envers la famille et l’école d’origine. La situation de soin est variable avec l’inclusion en premier lieu d’une pharmacopée naturelle afin de traiter le cancer et ses effets iatrogènes. Enfin, il subsiste une inhibition du partage social des émotions où, des attitudes de retenue et de silence chez les parents se manifestent durant tout le processus thérapeutique mais aussi en cas de décès de l’enfant. B. L’Occident Les pays occidentaux présentent une appréhension sociale et médicale singulière du cancer pédiatrique. La variabilité observée concerne l’organisation hospitalière et la communication médicale. Le praticien occidental établit une communication médicale ouverte avec l’enfant et sa famille et ce, dès le départ du processus thérapeutique. Le contexte curatif est caractérisé par une forte médication d’opioïdes afin de pallier aux nombreuses douleurs et effets des traitements. L’aménagement hospitalier permet la création de réseaux de soutien psychologique pour l’enfant et sa famille et l’établissement d’activités ludiques.(c) Mettre en exergue les diverses implications cliniques pour les professionnels de la santé L’intention de cette étude est de sensibiliser les professionnels de la santé à la pléiade de facteurs psycho-sociaux intervenant dans un contexte de maladie néoplasique. Éléments qui doivent requérir d’une bienveillance clinique où tant l’enfant traité que son entourage familial, doivent être entendus dans toute leur spécificité d’être humain. DISCUSSION Une stratégie d’action intéressante concernerait une grille de lecture culturelle du patient et de sa famille. Cette grille peut offrir un entendement de la diversité culturelle en psycho-oncologie et peut permettre au professionnel de réajuster finement son travail clinique dans un contexte quotidien de multiculturalité. Cette grille de lecture doit rester simple et proposer un petit nombre de « repères culturels » (items culturels) pour professionnels, leurs permettant d’ajuster certains paramètres cliniques (communication médicale, gestion des émotions, rapport à la mort, etc.). Cette considération ethnologique du patient doit supposer au préalable une formation de compétences dans le domaine de la culture et de la santé/maladie. [less ▲]

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See detailFunctional analysis of FRD3 in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Motte, Patrick ULg et al

Poster (2014, November 28)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

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See detailThe Beginning of UP in Northern Mongolia: New Results and Perspectives from the Open-air Site of Tolbor 16
Zwyns, N.; Bolorbat, T.; Flas, Damien ULg et al

Conference (2014, November 28)

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See detailEstimating Ventricular Stroke Work from Aortic Pressure Waveform
Kamoi, Shun; Pretty, Christopher; Chiew, Yeong Shiong et al

Poster (2014, November 28)

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See detailLes accumulateurs de voix
Remacle, Angélique ULg

Conference (2014, November 28)

Les troubles de la voix peuvent être engendrés tant par un malmenage que par un surmenage vocal. Tandis que le malmenage est causé par un geste vocal inadéquat, le surmenage résulte d’une charge vocale ... [more ▼]

Les troubles de la voix peuvent être engendrés tant par un malmenage que par un surmenage vocal. Tandis que le malmenage est causé par un geste vocal inadéquat, le surmenage résulte d’une charge vocale supérieure aux capacités de récupération du locuteur. La charge dépend principalement de la quantité de phonation en termes de durée, de fréquence fondamentale et de niveau de pression sonore de la voix. Ainsi, parler longtemps, fort et avec une voix aigüe peut mener au surmenage, principalement lorsque les conditions environnementales sont défavorables (mauvaise acoustique, bruit ambiant élevé, atmosphère sèche, etc). Depuis quelques années, des systèmes portables de quantification de la charge vocale sont commercialisés. Ces accumulateurs (ou dosimètres) objectivent le comportement vocal en situation réelle pendant une durée prolongée. Ils permettent de déterminer les moments auxquels le sujet parle, la durée de phonation, la fréquence fondamentale et le niveau de pression sonore de la voix. Certains modèles fournissent également des mesures du bruit environnant. Actuellement, les accumulateurs sont principalement utilisés chez les locuteurs professionnels, soit les personnes qui ne peuvent se passer de leur voix dans le cadre de leur travail, par exemple les enseignants et les chanteurs. La dosimétrie vocale permet de cibler les comportements vocaux à risque en contexte écologique ainsi que les situations favorisant la dysphonie. L’objectif des recherches utilisant ces outils est de proposer des lignes de conduites pour la prévention, l’évaluation et le traitement de la dysphonie, principalement chez les personnes ayant un usage vocal intensif. Aussi, ces recherches tentent d’établir des normes relatives à l’utilisation vocale journalière de différents groupes d’individus. En rééducation, les accumulateurs de voix peuvent également fournir un feedback vibratoire en temps réel lorsque des seuils d’intensité ou de fréquence, prédéfinis par l’expérimentateur avant l’usage, sont dépassés par le locuteur. [less ▲]

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See detailGuidance chez les professionnels de la voix
Remacle, Angélique ULg

Conference (2014, November 28)

Cet atelier s’intéresse aux locuteurs professionnels, soit les personnes qui ne peuvent se passer de leur voix dans le cadre de leur travail, par exemple les enseignants et les chanteurs. Outre la ... [more ▼]

Cet atelier s’intéresse aux locuteurs professionnels, soit les personnes qui ne peuvent se passer de leur voix dans le cadre de leur travail, par exemple les enseignants et les chanteurs. Outre la présentation de pistes relatives à la prévention et la prise en charge de cette population, l’atelier comporte des manipulations d'accumulateurs vocaux, y compris l'utilisation du feedback tactile dans le but de modifier le comportement vocal (contrôle de la fréquence et du niveau de pression sonore de la voix). Des moyens permettant de réduire la charge vocale et le bruit environnant sont également présentés et manipulés : système d’amplification vocale et traqueur de bruit. [less ▲]

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See detailQuels modèles pour soutenir la prévention et la promotion de la santé ?
Vanmeerbeek, Marc ULg; Mathonet, Julien; Miermans, Marie-Christine ULg et al

Conference (2014, November 28)

Contexte et objectifs La prévention n’atteint pas souvent son objectif d’accès universel aux soins préventifs. Les modèles opérationnels publiés concernant la prévention et la promotion de la santé en ... [more ▼]

Contexte et objectifs La prévention n’atteint pas souvent son objectif d’accès universel aux soins préventifs. Les modèles opérationnels publiés concernant la prévention et la promotion de la santé en soins primaires ont été recherchés pour réaliser un inventaire fonctionnel et formuler des hypothèses de travail sur l’efficacité et l’équité de la prévention. Méthode Revue narrative de littérature utilisant les mots-clés relatifs aux différents niveaux de prévention, à la promotion de la santé, aux soins primaires, aux modèles de pratique et à la délivrance de soins. Une analyse multicritères a été utilisée. Principaux résultats Douze modèles ont été sélectionnés. Les modèles dont les auteurs annoncent qu’ils s’appliquent à la prévention décrivent majoritairement des approches centrées sur les individus dans le cadre de la relation médecin-patient, prenant en compte l’organisation de pratique. Certains modèles systémiques de type socio-écologique illustrent le concept de promotion de la santé : pratique éducative, ciblage sur des groupes ou des populations, prise en compte des milieux de vie et des déterminants sociaux de la santé. La place des patients dans l’élaboration des modèles est mineure, à l’image de leur implication comme acteurs dans les systèmes de soins. Les définitions des termes de prévention, promotion de la santé et éducation du patient diffèrent considérablement d’un modèle à l’autre. Discussion et conclusion Certains éléments valorisés par la promotion de la santé seraient intégrables aux pratiques de soins : empowerment individuel des patients, prise en compte de leurs milieux de vie ; participation accrue à des réseaux locaux de professionnels de la santé ; intégration, au sein des mêmes structures, de l’approche individuelle et collective pour permettre la poursuite simultanée des objectifs de personnalisation, d’efficacité et d’équité. Ces évolutions ont des implications sur la formation des soignants, en termes d’aptitudes communicationnelles, de sensibilisation aux concepts de la santé publique et d’immersion précoce des futurs soignants dans la communauté. [less ▲]

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See detailEstimating Ventricular Stroke Work from Aortic Pressure Waveform
Kamoi, Shun; Pretty, Christopher; Chiew, Yeong Shiong et al

in 13th Belgian Day on Biomedical Engineering (2014, November 28)

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See detailTracking stressed blood volume during vascular filling experiments
Pironet, Antoine ULg; Dauby, Pierre ULg; Chase, J. Geoffrey et al

Poster (2014, November 28)

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac ... [more ▼]

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac output after fluid infusion. [less ▲]

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See detailTracking stressed blood volume during vascular filling experiments
Pironet, Antoine ULg; Dauby, Pierre ULg; Chase, J. Geoffrey et al

in 13th Belgian Day on Biomedical Engineering (2014, November 28)

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac ... [more ▼]

A three-chamber cardiovascular system model is used to compute stressed blood volume from filling experiments. As previously observed, stressed blood volume is a good predictor of the change in cardiac output after fluid infusion. [less ▲]

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See detailL’animation théâtrale comme présence sociale
Brahy, Rachel ULg

Conference (2014, November 27)

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See detailPPP in the field of employment: clandestine strategies to become an efficient PPP?
Remy, Céline ULg

Conference (2014, November 27)

In this article, we study the Public-Private Partnerships (PPP) in the field of employment, in Belgium. These PPPs are established between Public Employment Service (PES) and private operators to create ... [more ▼]

In this article, we study the Public-Private Partnerships (PPP) in the field of employment, in Belgium. These PPPs are established between Public Employment Service (PES) and private operators to create projects for support and training for jobseekers the ultimate goal of which is social and professional reintegration. There are three critical moments in the life of the partnership: getting a partnership agreement with the PES, mobilizing jobseekers to participate in the delivery, and evaluating the outcomes achieved by the operator. The parties affected by these three critical steps are mainly the private operators. To overcome these difficulties, the providers implement informal and "clandestine" strategies to ensure the proper operation of the partnership and especially its performance and sustainability. [less ▲]

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See detailDiscussant Panel on "Public-Private Partnerships: New Governance Arrangements"
Remy, Céline ULg

Conference (2014, November 27)

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See detailRole of the polyQ length and non-polyQ regions during the aggregation process into amyloid fibrils of model polyQ proteins
Huynen, Céline ULg; Willet, Nicolas ULg; Buell, Alexander K et al

Poster (2014, November 26)

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated ... [more ▼]

Nine neurodegenerative disorders, referred to as polyglutamine diseases and including Huntington’s disease, are associated with the abnormal expansion of a polyglutamine tract inside nine unrelated proteins. This polyQ expansion is thought to be the major determinant in the development of neurotoxicity, triggering protein aggregation into amyloid fibrils. A large body of evidence however suggests that non-polyQ regions modulate the aggregation process triggered by polyQ expansions. The interplay between the polyQ tract and non-polyQ regions is complex and still not fully understood. In order to better understand it, we previously designed and characterized model polyQ proteins made of the beta-lactamase BlaP with 23, 30, 55 and 79Q inserted at position 197 or 216. Our first results had indicated that our model is relevant to study polyQ aggregation since it recapitulates the aggregation properties of polyQ disease-associated proteins: there is a Q-threshold for the spontaneous formation of amyloid fibrils in solution, and above the threshold, the longer the polyQ, the faster the aggregation. Moreover, the structure of BlaP and the position of insertion of the polyQ tract influence their aggregation properties in solution. This work aims to better understand, at the molecular level, (i) the precise role of the polyQ length (23, 30, 55, 61, 67, 73 and 79Q), (ii) the conformation of the host protein (native or unfolded BlaP), (iii) the location of the polyQ tract within BlaP (197 or 216), (iv) the flexibility of the polyQ flanking sequences, and (v) the origin of constraints applied by BlaP to the inserted polyQ tract (at its N- or C-terminal end) on the structural, thermodynamic and aggregation properties of BlaP-polyQ chimeras, using a wide range of biophysical techniques (e.g., spectroscopy methods, quartz crystal microbalance, atomic force microscopy and dynamic light scattering). The effect on the aggregation properties will be determined on the spontaneous aggregation into amyloid fibrils in solution, and on the nucleation and on the elongation steps of amyloid fibril formation. For this purpose, new chimeras containing 61, 67 and 73Q at position 197, or 55Q inserted at position 197 in between two different protease’s cleavage sites, that are relatively flexible, will be moreover created. Our results first demonstrate that the spontaneous aggregation into amyloid fibrils in solution is correlated to the polyQ length with an exponential growth function, and that the elongation rate is linearly correlated to the polyQ length, independently of the protein context (i.e., conformation of BlaP, and/or location of the polyQ tract, and/or polyQ peptides inserted or not within BlaP). However, the location of the polyQ tract inside BlaP, and/or its conformational state, and/or the flexibility of polyQ flanking sequences, and/or the origin of constraints applied to the polyQ tract drastically influence the ability of a polyQ tract to trigger the nucleation and/or the elongation step of amyloid fibrils (variation in the Q-threshold and in the absolute rate of both steps). Altogether, our results suggest that non-polyQ regions constitute an additional potential therapeutic target, more specific than drugs targeting the polyQ sequence, to interfere with the nucleation and/or the elongation of amyloid fibrils, associated to neurotoxicity. A possible drug could be constituted by a ligand specific to non-polyQ regions of disease-associated proteins, which further increases the constraints applied to the polyQ expansion to prevent the disease onset and/or progression. [less ▲]

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See detailAllergies alimentaires
GADISSEUR, Romy ULg

Conference (2014, November 26)

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See detailGuides d'architecture moderne et contemporaine en Wallonie et à Bruxelles
Charlier, Sébastien ULg; Moor, Thomas

Conference (2014, November 25)

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See detailUse of 13C-NMR in structural elucidation of polysaccharides: case of locust bean gum
Gillet, Sébastien ULg; Aguedo, Mario ULg; Blecker, Christophe ULg et al

Poster (2014, November 25)

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic ... [more ▼]

Locust bean gum (LBG) galactomannans are polysaccharides consisting of a β-(1→4) D-mannopyranosyl backbone substituted to varying degrees in α-(1→6) with single D-galactopyranosyl residues. This basic structure is the same for all galactomannans (Fig. 2). However, when locust bean gum is extracted at different temperatures, the generated fractions exhibit different properties in aqueous solution (viscosity, viscoelasticity, gel formation, thermohydrolysis resistance, etc.). This means that there are differences within the fine structure of the polymers (although the basic structure is the same). Analysis of [13C]-NMR spectra of galactomannans, in combination with other techniques, can provide capital information about fine structural elucidation of the polymers. The method specifies the distribution of lateral galactosyls along the main chain of mannans. Two fractions extracted from locust bean gum at 25 and 80 °C (respectively GM25 and GM80) were comparatively studied by [13C]-NMR. Mannosyls/Galactosyls (M/G) ratios can be determined by considering the intensities of C-1 mannose and galactose signals in [13C]-NMR spectra. This method provides results relatively close to those obtained by GC-MS analysis. Spectra also showed that resonance from C4 of D-mannose residues were split, in evident dependence upon the nearest-neighbor probabilities (“diad frequencies”) of D-galactosyl groups along the mannan chains (Fig. 2). Diad frequencies were obtained by integrating C4(Man) peak areas. F11, F21/F12 and F22 gave respectively the di-, mono- or non-substituted mannose pairs proportions. High percentages of F11 and F22 therefore indicate a more non-homogeneous distribution of lateral galactosyls along the polysaccharide backbone as observed for GM80. The percentages of total lateral substituents obtained by C4(Man) peak analysis [F11 + (F21 or F12)/2] were fairly well correlated with M/G ratios. Splitting of the C-6 substituted D-mannose resonance provides, therefore the basis for determining the next-nearest-neighbor probabilities (triad frequencies) (Fig. 2). However, the spectrum is often not sufficiently resolved to accurately quantify and interpret the results. [less ▲]

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