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See detailEffect of encapsulated nanoparticles on thermophillic anaerobic digestion
Al-Ahmad, Alaa Eddin ULg; Hiligsmann, Serge ULg; Lambert, Stéphanie ULg et al

Poster (2014, February 07)

Recently, enormous interest has been focused on biological applications of metal nanoparticles NPs due to their small size, high specified surface and their great potential in application to many science ... [more ▼]

Recently, enormous interest has been focused on biological applications of metal nanoparticles NPs due to their small size, high specified surface and their great potential in application to many science fields. The most studied process concerns zero valent palladium and iron NPs improving anaerobic biodegradation of chlorinated hydrocarbons (Windt et al., 2005). Moreover, investigation carried out in our lab showed that iron NPs encapsulated in silicate matrix may enhance hydrogen production by Clostridium butyricum (Beckers et al., 2013). Nevertheless the influences of metal NPs on methane producing anaerobic digestion have seldom been investigated. The present work investigates the enhancement effect of seven different metal NPs on methane production during the thermophilic anaerobic digestion. NPs of Cu, Pd, Pt, Ni, Co, Ag and Fe encapsulated in porous silica (SiO2) to prevent their coagulation and agglomeration, were added at concentration of 10-5mol/L in batch test (125ml serum bottles containing 70mL culture medium with 5g/L acetate monohydrate as the sole carbon substrate). Nickel, cobalt and iron NPs improved methane production from acetate. To confirm the previous results, the NPs were tested at different concentrations (10-4, 10-5, and 10-6 mol/L) with starch and glucose substrates. The results show that the impact increases with the increase of NPs concentrations up to 10-4 mol/L. The modified Gompertz equation was applied to describe the effect of NPs on anaerobic digestion. According to this model, the kinetic of methane production was particularly affected by nanoparticles addition. The values of the maximum methane production rate MPR (ml/day) was significantly higher 72.5% with nickel NPs at a concentration of 10-4 mol/L than the control without NPs. [less ▲]

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See detailA diachronic analysis of the dynamic of two cities: Kisangani and Lubumbashi (Democratic Republic of Congo): How do the extension and/or of the densification of these cities impact the urban internal and peripheral ecosystems?
Andre, Marie ULg; Mahy, Grégory ULg; Lejeune, Philippe ULg et al

Poster (2014, February 07)

The dynamics of two cities between two years (2002 and 2010 for Kisangani; 2002 and 2008 for Lubumbashi, both part of the Democratic Republic of Congo) have been examined in order to estimate how their ... [more ▼]

The dynamics of two cities between two years (2002 and 2010 for Kisangani; 2002 and 2008 for Lubumbashi, both part of the Democratic Republic of Congo) have been examined in order to estimate how their extension and/or of the densification have impacted the urban internal and peripheral ecosystems. This study singularizes itself from the subjective ones by its quantified and objective approach based on landscape ecology. This discipline assumes that the landscape pattern description is important because, according to its central hypothesis called “pattern-process paradigm”, the ecological processes influence landscape structure and vice versa. Composition metrics, which are commonly used in landscape ecology, describe the pattern among other things in quantifying the presence and the number of patches from the various classes within the landscape. For each city two SPOT 5 images were classified with a oriented-object supervised approach; then built area proportion index, a composition landscape metric, has been evaluated in order to 1) identify the extent of each area (urban, suburban, rural) within the urban-rural gradient, 2) quantify the dynamic of the different areas in the urban-rural gradient during the last decade and 3) quantify the effect of a decade of urban and suburban growth on ecosystems. The similarities and differences between these cities, relevance and gaps in the method have then been identified and discussed. [less ▲]

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See detailEstimating daily yield and content of major fatty acids from single milking
Arnould, Valérie ULg; Reding, Romain; Delvaux, Charles et al

Poster (2014, February 07)

Reducing the frequency of milk recording and the number of recorded samples per test-day could be a solution in order to reduce costs of official milk recording. However, fewer samples lead to a decrease ... [more ▼]

Reducing the frequency of milk recording and the number of recorded samples per test-day could be a solution in order to reduce costs of official milk recording. However, fewer samples lead to a decrease in the accuracy of predicted daily yields. Unfortunately, the current published equations use the milking interval that is often not available and/or reliable in practice. The first objective of this study was to propose models using easily available traits. Therefore the milking interval was replaced by a combination of data easily recorded by milk recording. The second objective of this study was to enlarge the previous investigations to milk fatty acids (FA) in order to propose a practical method for estimating accurate daily milk, fat and major FA yields from single milking. The fit goodness of proposed models was evaluated based on the correlation values between the estimated and observed daily yields in addition to the calculation of the mean square error. Obtained results are promising. Correlation values were comprised between 96.4% and 97.6% when daily yield were estimated from morning milking, and from 96.9% to 98.3% when daily yield were estimated from evening milking. The combination of records related to lactation stage, month of test, milk yield, and fat could replace the milking interval effect. Because of their simplicity, proposed models would be easy to implement. [less ▲]

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See detailOld inks: pigments extracted from plants
Despy, Jessica; Wymeersch, Noémie; Bouchat, Isabelle et al

in BOOK OF SHORT ABSTRACTS - POSTER PRESENTATIONS 19TH NATIONAL SYMPOSIUM ON APPLIED BIOLOGICAL SCIENCES (2014, February 07)

Thousands of years ago, natural pigments were discovered and they have been used ever since. Indeed, prehistoric people already used them to paint the walls of the caves in which they were living. A ... [more ▼]

Thousands of years ago, natural pigments were discovered and they have been used ever since. Indeed, prehistoric people already used them to paint the walls of the caves in which they were living. A significant example of this is the Cosquer cave (-19,000 to -27,000 years) located near Marseilles. Pigments and dyes can be classified into two broad categories and five families: natural pigments and dyes and those called artificial. The first one of these five families includes the mineral pigments. Among these we can find the clays (yellow ochre, red ochre, green clay, brown clay) and the stones like lapis lazuli (blue). The second and third families gather the organic dyes and pigments. Those that have vegetal origins like indigo (blue) and madder (red) compose the second family and those that have animal origins like cochineal (red) and kermes dyers (carmine) form the third family. One family includes pigments and dyes stemming from chemical reactions such as verdigris or red lead (family 4) and the other one is made of the miscellaneous inks such as iron-gall type who are vegetal and mineral one (family 5). All these pigments, although they have been used for centuries, have been replaced by synthetic dyes from the oil industry at the end of the 19th century. Indeed, they have the advantage of reproducibility of the properties unlike natural pigments. But the scarcity of oil causes a renewed interest in natural preparations. We report the extraction of pigments from plants. [less ▲]

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See detailProfiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages
Druart, Philippe; Michels, Franck ULg; Misson, Jean-Pierre et al

Poster (2014, February 07)

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Peer Reviewed
See detailInteractions of a potential plant elicitor mannolipid with plant model membranes
Polo Lozano, Damien ULg; Lins, Laurence ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act ... [more ▼]

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. The target of the elicitors is supposed to be the plant plasma membranes (PPM). The main mechanisms of interaction of many elicitors involve proteic receptors but lipid-based elicitors (LBE) may preferably interact with the lipidic fractions of PPM. However there is no detailed information at the molecular level on the PPM-LBE interactions. Our work is focused on a original synthetic LBE composed of a mannoside linked to a myristic acid. It has potential elicitor activities as shown by the assays on tobacco root cells. These activities could be related to its interaction with the lipidic phase of PPM. Since PPM are complex entities, the analyses of the PPM- molecule interactions are quite difficult. In this context, these interactions were carried out using biomimetic membranes of PPM such as Langmuir monolayers and multilayers. The effects of our molecule on these membranar systems were investigated by biophysical and in silico approaches. [less ▲]

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See detailIs the behaviour of grazing cattle influenced by the presence of a hedge in the pasture?
Vandermeulen, Sophie ULg; Yando, Emile; Marche, Christian et al

Poster (2014, February 07)

The interest in using shrubs and trees as forage for cattle is raising in temperate regions, along with the environmental measures promoting hedges along pasture. This study aimed at determining the ... [more ▼]

The interest in using shrubs and trees as forage for cattle is raising in temperate regions, along with the environmental measures promoting hedges along pasture. This study aimed at determining the influence of the presence of a woody hedge on grazing cattle behavior as well as the evolution of this behavior during the grazing season. Twelve heifers were set to graze a standard ryegrass and clover meadow during the grazing season of 2013. The animals were divided in 2 groups : one with a free access to a hedge composed by 10 temperate shrub and tree species, the other with no access to a hedge (control). Their behavior (grazing, browsing and other activities) was monitored during 14h d-1 replicated 3d week-1 during 3 periods (May, July and September). Results suggest that both season and presence of a hedge have an influence on the behavior of heifers. Grazing, resting and rumination varied between the two groups in each period (P<0.05). The time spent browsing was influenced by the season (P<0.001); the heifers with an access to the hedge could browse 19.3% of the total time in May against 5.9 and 5.4% in July and September. Both control and experimental heifers grazed more with the progressing season, but the animals without the hedge grazed always more than the heifers that could graze and browse (49.6 vs 43.0%). In conclusion, browsing woody species represents a significant part of the time spent for forage ingestion, and variation in this behavior over the whole grazing season requires to investigate the influence of both pasture and browse availability and composition. [less ▲]

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See detailField bordering flower strips as source of lipids
Paul, Aman ULg; Danthine, Sabine ULg; Stephanie, Heuskin et al

Poster (2014, February 07)

Field bordering flower strips not just only improves the biodiversity but also serves as a source of beneficial compounds. Some of the plants in these strips can be really interesting source of lipids ... [more ▼]

Field bordering flower strips not just only improves the biodiversity but also serves as a source of beneficial compounds. Some of the plants in these strips can be really interesting source of lipids, the oils extracted from their seeds can be important for food, pharmaceutical and cosmetics industries. Six species of plants from flowering strips in Belgium were investigated for their seed oil content. The oil from seeds was extracted by cold extraction technique using chloroform/methanol in 2:1 ratio as solvent. Oil extraction from seeds of Red Clover (Trifolium pratense), Rough Hawkbit (Leontodon hispidus), Cow Parsley (Anthriscus sylvestris), St John’s Wort (Hypericum perforatum), Common Yarrow (Achillea millefollium) and Birdsfoot Trefoil (Lotus corniculatus) plant species was done on wet weight which came out to be 7.89±0.11%, 11.86±0.07%, 14.78±0.31%, 24.20±0.02%, 20.08±0.15% and 7.04±0.12% respectively. The physicochemical properties of the extracted oils were analyzed. Some of these oils can be of great commercial value. [less ▲]

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See detailAnd the winner is ... When =f and pAj=f play hide-and-seek
Winand, Jean ULg

Conference (2014, February 07)

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See detailRealized niche of eight endemic plant species of Katangan Copperbelt (D.R. Congo): implications for copper species conservation
Boisson, Sylvain ULg; Seleck, Maxime ULg; Lebrun, Julie et al

Poster (2014, February 07)

South of the Katanga province (D.R. Congo), a unique vegetation grows on soils developed on 100 Cu-Co outcrops among the most important in the world (katangan Copperbelt). These outcrops form of isolated ... [more ▼]

South of the Katanga province (D.R. Congo), a unique vegetation grows on soils developed on 100 Cu-Co outcrops among the most important in the world (katangan Copperbelt). These outcrops form of isolated hills in the landscape hosting vegetal communities evolving along the mineralization gradient. More than 600 metallophytes can be found in these communities with some 32 strict endemics solely known from mineralized soils. These species present physiological and biological adaptations allowing them to tolerate toxic levels of metals in soils. The unique characteristics of most endemics are seriously threatened by the intense mining activities in the region thus also threatening the high genetic potential to be used in development of polluted soils’ restoration strategies. In an effort to characterize the ecology of these species, we model ecological niches of eight endemic species along copper-cobalt gradients. For each species, three sites were prospected and transects set. Along these, five plots where the species occurred were selected: at both the extremities of the gradient and at 1st quartile, median and 3rd quartile. In each plot, individuals of the particular species were counted and composite soil samples collected. Available EDTA Cu-Co values were determined and niches modeled for both metals through the kernel density method in R 3.0.1 (package ‘vioplot’). Results show that ecological niches of the selected endemics are distributed between Cu-soil values of 50 and 11 000 mg.kg-1 and between Co values between four and 1500 mg.kg-1. Six species occur in the lower 100 mg.kg-1concentrations of Co. As for the Cu gradient, two species present ecological optimums over 3000 mg.kg-1. This variation of ecological niches along the gradients indicates a need of adequacy between species conservation strategies and soils’ metal contents. Four of the eight studied species should be conserved on soils contaminated with some 500 mg Cu.kg-1 and 30 mg Co.kg-1. For others, precise conservation actions need to be undertaken. [less ▲]

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See detailBiofilms from entomopathogenic fungi in mosquito control
Bawin, Thomas ULg; Boukraa, Slimane; Seye, Fawrou et al

Poster (2014, February 07)

Mosquitoes (Diptera: Culicidae) are zoonotic vectors of medical and veterinary importance. As part of an integrated vector control, metabolites secreted by entomopathogenic fungi could be developed as ... [more ▼]

Mosquitoes (Diptera: Culicidae) are zoonotic vectors of medical and veterinary importance. As part of an integrated vector control, metabolites secreted by entomopathogenic fungi could be developed as biopesticides. In this context, filamentous microorganisms growing on a support as biofilm in a liquid medium would offer several advantages in bioreactor regarding performances and metabolites recovery. The production of toxic metabolites by an entomopathogenic fungus Aspergillus flavus in such conditions was assessed. Three initial inoculum levels, i.e. 10^1, 10^3 and 10^6 spores/ml of PYG medium, have been tested in shake flask with or without support. Toxicity tests were performed on Culex quinquefasciatus larvae using dilutions of 1, 2, 4, 6, 8 and 10% of liquid cultures. The results indicated that A. flavus tends to form pellets in submerged culture; the size and the amount of pellets was affected by the initial inoculum level of spores. Under similar conditions, the filaments fixed on a support and didn’t appear in free form in the liquid. Toxicity tests revealed differences between both free and fixed forms. All combined conditions, LC50s ranging up to dilutions of 2.2 and 4.8% were observed within 48 hours. Secretomes could be compared between these culture conditions by proteomic and metabolomic approaches. [less ▲]

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See detailIs the noxious Ambrosia artemisiifolia L. going to invade Belgium?
Ortmans, William ULg; Chauvel, Bruno; Mahy, Grégory ULg et al

Poster (2014, February 07)

Ambrosia artemisiifolia L. is an invasive species from North America, causing a health crisis in Europe due to its highly allergenic pollen. In France, there is a zone where the A. artemisiifolia ... [more ▼]

Ambrosia artemisiifolia L. is an invasive species from North America, causing a health crisis in Europe due to its highly allergenic pollen. In France, there is a zone where the A. artemisiifolia populations are naturalized and invasive. Outside this area, populations are more rare and do not seem to expand. We tested if the performances of A. artemisiifolia are varying with competition level, and among geographical zones. The results show that populations from Belgium and Netherlands are not less efficient than invasive populations for the measured traits. [less ▲]

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See detailImpact of abiotic stresses on volatile organic compound production of field crops and grasslands
Digrado, Anthony ULg; Mozaffar, Ahsan ULg; Bachy, Aurélie ULg et al

Poster (2014, February 07)

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on ... [more ▼]

Abiotic and biotic stresses are known to alter biogenic volatile organic compound (BVOC) emission from plants. With the climate and global change, BVOC emissions are likely to increase. This increase on BVOC emissions could be driven by many environmental parameters like temperature, ozone and light availability for photosynthesis although it is still difficult to predict the impact of some environmental parameters, environmental controls on BVOC emission being species and BVOC-dependent. These BVOC are involved in a wide range of interactions of plants with their environment and these interactions could be affected by the global change. Moreover, BVOC also play a key role in the atmospheric chemistry and may contribute to ozone formation and an increase in methane lifetime, strengthening the global change. Yet, due to technical limitation, there are few studies examining the impact of multiple co-occurring stresses on BVOC emission at the ecosystem level although stress combination is probably more ecologically realistic in field. In the CROSTVOC (for CROp STress VOC) project, the impact of abiotic stresses (e.g. heat, drought, ozone and grazing) on BVOC emission will be investigated for field crops (maize and wheat) and grassland both at the ecosystem and plant scale. [less ▲]

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See detailUse of Near Infrared Hyperspectral Imaging (NIR-HSI) and chemometric tools to dicriminate wheat roots and straws in soil
Eylenbosch, Damien ULg; Fernandez Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2014, February 07)

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See detailDevelopment of formulated elicitors to control bioagressors of wheat: which interests?
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now ... [more ▼]

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now. However, the environmental and health issues caused by these chemicals make it urgent to develop safer methods for pathogen control. In the optic of a sustainable agriculture, several biocontrol tools have been developed during the last decades. Elicitors are increasingly subject to research as interesting alternatives to phytosanitary products. Yet, few efficient formulations exist on the market, and no product has been designed specifically to protect wheat crops. This study will thus focus on the the manipulation of host natural defenses through the use of elicitors as a preventive treatment against major wheat diseases such as Septoria tritici, Fusarium graminearum and Fusarium culmorum. [less ▲]

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See detailQuantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age
Delory, Benjamin ULg; Delaplace, Pierre ULg; du Jardin, Patrick ULg et al

Poster (2014, February 07)

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In ... [more ▼]

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering. [less ▲]

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See detailUsing micro-injection technique to assess fungal toxicity in mosquito control
Bawin, Thomas ULg; Boukraa, Slimane; Seye, Fawrou et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07), 79(1), 181-185

Topical application of insecticidal compounds allows directly exposing these substances on insect tissues and measuring their toxicity while ignoring many factors. However, this technique remains ... [more ▼]

Topical application of insecticidal compounds allows directly exposing these substances on insect tissues and measuring their toxicity while ignoring many factors. However, this technique remains difficult to apply on mosquito larvae considering their aquatic lifestyle. Micro-injection could be used for the direct deposition of toxic compounds in the larvae. Capillaries exhibiting an injection tip with an external diameter of 0.5 mm have been designed from silica tubes. For each treatment, a capillary is mounted on a pump connected to a flow rate regulator. Culex quinquefasciatus larvae were injected with 10^7 spores/ml of entomopathogenic fungi (Aspergillus clavatus, Metarhizium anisopliae, Metarhizium sp.). Mortalities were recorded daily during 72h. The distribution of spores stained with methylene blue and injected into the body of larvae was also observed according to the system described. Results showed that spores were distributed over the whole body. The injection of Aspergillus clavatus, Metarhizium anisopliae and Metarhizium sp spores induced corrected mortalities of 62%, 53% and 57% after 72h, and differed statistically from control groups. Finally, post-mortem emergences of filaments from dead larvae were observed in the case of the three fungal strains confirming spore viability. Injection of inactivated spores (or inert bodies of similar size) could help to reject the hypothesis of a response due to the presence of foreign bodies. [less ▲]

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See detailEffect of sun-drying on flavonoïd composition and antioxidant activity of three dark fig varieties
Bachir Bey, M.; Richard, Gaetan ULg; Fauconnier, Marie-Laure ULg et al

Poster (2014, February 07)

The aim of the study was to investigate the effect of sun-drying on individual flavonoïd and antioxidant activities of three dark fig varieties (Azenjar, Bouankik, and Aberkane). Flavonoïd compounds were ... [more ▼]

The aim of the study was to investigate the effect of sun-drying on individual flavonoïd and antioxidant activities of three dark fig varieties (Azenjar, Bouankik, and Aberkane). Flavonoïd compounds were identified and quantified using high-performance liquid chromatography. The results showed that all samples significantly differed in their flavonoïd compositions. [less ▲]

Detailed reference viewed: 37 (4 ULg)
See detailSimulations of a beta amphiphilic peptide as potential surfactant of membrane proteins
Crowet, Jean-Marc ULg; Dony, Nicolas ULg; Deschamps, Antoine et al

Poster (2014, February 07)

The peptide studied here was designed to form beta amphiphilic films with the aim to stabilize purified membrane proteins. This interaction has notably been followed by FRET. Hydrophobic and hydrophilic ... [more ▼]

The peptide studied here was designed to form beta amphiphilic films with the aim to stabilize purified membrane proteins. This interaction has notably been followed by FRET. Hydrophobic and hydrophilic residues are alternate and positively and negatively charged residues place respectively at the start end the end of the peptide. The peptide has been studied by atomistic and coarse grained molecular dynamics in water, chloroform and mixed solutions. The peptide was observed to spontaniously form beta films at the chloroform water interface. Moreover, when we simulate the interaction of this peptide with a membrane protein and with a membrane protein in a micelle of dodecylphosphocholine. The peptide was observed to form beta films at the membrane protein surface and even remove surfactants from the membrane protein surface. The simulations confirms the behaviour of this peptide observed in vitro and shows that it could be used instead of detergents. [less ▲]

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See detailThe emergence of the possessive article pAy.f before the Amarna period
Sojic, Nathalie ULg

Conference (2014, February 07)

This presentation gives an overview of my research devoted to the Late Egyptian possessive article pAy.f. It aims at examining the uses of an element whose meaning evolved through time. Rather than ... [more ▼]

This presentation gives an overview of my research devoted to the Late Egyptian possessive article pAy.f. It aims at examining the uses of an element whose meaning evolved through time. Rather than looking for the very first occurrences of pAy.f in the Old Kingdom texts, my main concern is to analyze closely its progressive spread as well as to outline the gradual steps of its semantic evolution across the pre-amarnian corpus (15th-17th Dyn., c. 1650-1350 b.C.). On the one hand, I highlight the process by which this recent construction gradually encroaches on the older one, and on the other hand, I define the the meanings of pAy.f in relation to its distribution according to text genres. [less ▲]

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See detailarchiDART: a R package allowing root system architecture analysis using Data Analysis of Root Tracings (DART) output files
Delory, Benjamin ULg; Baudson, Caroline ULg; Brostaux, Yves ULg et al

Poster (2014, February 07)

In 2010, Le Bot et al presented a free and open-access software (Data Analysis of Root Tracings - DART) allowing the analysis of complex root system architectures from captured images, particularly across ... [more ▼]

In 2010, Le Bot et al presented a free and open-access software (Data Analysis of Root Tracings - DART) allowing the analysis of complex root system architectures from captured images, particularly across time series. Using this software, a user has to manually identify roots as a set of links. After vectorization of a root system, three final data sets (RAC, TPS and LIE) can be exported as table files containing several attributes for (a) each individual root (e.g. root length), (b) each observation day or (c) each point used to construct the vectorized root system respectively. These data sets can finally be used either to calculate derived root system architecture (RSA) parameters or to draw the root system architecture at selected observation dates. However when an experiment involves the analysis and comparison of many root systems, the calculation of RSA parameters for each data set and the drawing of the corresponding vectorized root systems become time-consuming. In this context, we developed a R package, called archiDART, allowing both the automatic calculation of common root architecture parameters and the X-Y plotting of vectorized root systems for selected observation dates. [less ▲]

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See detailBiofilter in aquaponics
Delaide, Boris ULg; Lambrechts, Paul Emile ULg; Willaert, Jean-François ULg et al

Poster (2014, February 07)

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then ... [more ▼]

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then are available for plants. Wastewater from aquaculture is filtrated by plants and return to the fish tank in a loop cycle. This strongly reduces the use of water and increases the economic efficiency with an additional production of plant, e.g. vegetables. From the hydroponics view, this combination allows important decreases in chemical nutrients use for plant grow. A central part of this system is the biofilter. The microorganisms that colonize it are essential to process the fish waste into soluble nutrients easily assimilated by plants. During this process, nitrifying bacteria convert free ammonia into nitrite and then nitrate. The first objective of this research will concern the identification and isolation of the main microorganism species involved by the dilution plating and metagenomics technics. The optimal conditions to process the fish waste will be determined for such microorganism. The efficiency of nitrite and nitrate production by these micro-organisms will be tested in different conditions (T°, pH, O2, water flow, support, etc..) in vitro at the lab scale and in aquaponics prototypes. [less ▲]

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See detailSreening of essential oils against rice pathogens isolated from Madagascar
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2014, February 07)

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas ... [more ▼]

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae and the fungi Alternaria sp. and Curvularia lunata. These pathogens are causing significant yield reduction on rice, which is the staple food in this country. After the identification of those pathogens, the objective of this work was to identify appropriate control measures against them. Plants extracts has been traditionally used to control diseases in other plant species. Nowadays, the problem of residues of phytopharmaceutical product, reinforce the need for research on the development of natural plant extracts to control plant diseases. Madagascar has many endemic plant of interest and we have evaluated the antimicrobial properties of essential oils from Malagasy plants. The main object of this study is the in-vitro screening of essential oils to control the pathogens isolated on rice in Madagascar. Thirty nine essentials oils from Madagascar had been tested. Activity of essentials oils on Pseudomonas fuscovaginae, Alternaria sp. and Curvularia lunata had been tested using broth media and on agar medium for Xanthomonas oryzae pv. oryzae. The growth of the pathogen (bacteria and conidia) in the presence of the essential oil was evaluated and compared to a control. Nine of the 39 essentials oils showed interesting antimicrobial activity with an efficiency more than 70% against at least one bacterial pathogen. One essential oil inhibited the growth of the two pathogens tested. For fungal pathogens, three essential oils presented an efficiency of more than 70% against at least one fungal pathogen. These results confirm that some essential oils present an interesting antimicrobial activity. The in-situ confirmation on plant of this activity is currently ongoing. [less ▲]

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See detailIncrease in Soil Macroporosity managed with Winter Ploughing - a preliminary results
Parvin, Nargish ULg; Chelin, Marie ULg; Hiel, Marie-Pierre ULg et al

Poster (2014, February 07)

Measurement of soil water retention capacity by the conventional pressure plate technique always gives a substantial view of soil porosity distribution. The structural orientation is observed in the ... [more ▼]

Measurement of soil water retention capacity by the conventional pressure plate technique always gives a substantial view of soil porosity distribution. The structural orientation is observed in the beginning (higher water retention at 9.8 to 98 hPa water head pressure indicates greater proportion of macroporosity) of the soil moisture characteristic curve obtained from the water retention measurement. Since, tillage practices generally increase soil porosity, the correlation between soil hydraulics and porosity distribution would expect to be different for different tillage systems. In general, macroporosity increase with the adoption of conservative tillage or no tillage system but the changes can be varied with the seasonal variation. In our study, winter ploughing retains more water at the range of 9.8 to 98 hPa than Strip tillage, No-till residues in and No-till residues out. So, we can conclude that there is certainly increase in macroporosity in ploughing than other conservation practices of reduced tillage and no tillage. [less ▲]

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See detailUse of lipases for the kinetic resolution of lactic acid esters in heptane or in a solvent free system
Richard, Gaetan ULg; Nott, Katherine; Nicks, François et al

Poster (2014, February 07)

This poster illustrates the kinetic resolution of lactic acid esters using CAL-B as catalyst. The racemic mixture is resolved in heptane and even in a solvent free system.

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See detailThree aspects, One concept: Agroecology. Agroecological practices and human interactions for a new approach for science. An example at the Univeristy of Liege.
Hatt, Séverin ULg; Artru, Sidonie ULg; Boeraeve, Fanny et al

Poster (2014, February 07)

Critics are raising about conventional farming and its consequences on biodiversity, human health and society. As alternatives, novel models for agriculture are proposed, and among them Agroecology. Quite ... [more ▼]

Critics are raising about conventional farming and its consequences on biodiversity, human health and society. As alternatives, novel models for agriculture are proposed, and among them Agroecology. Quite often, Agroecology is seen as the application of ecological knowledge to the agricultural production. Indeed, this helps to develop more ecological farming practices favoring biodiversity to provide ecosystem services at multiple scales. Agroecology goes further in considering that the agricultural production is integrated in a food system guided by human interactions. This latter one takes into account socio-economic and political dimensions to develop new production systems. Doing so, it assures food security worldwide while preserving resources for future generations. Facing these ambitious objectives, academics are invited to elaborate a new approach for science in developing participatory and action-oriented approaches as well as multidisciplinarity. AgricultureIsLife is a research platform built up at the University of Liège (ULg). In 2013, 40 researchers (including 18 young researchers) from 16 research units of ULg were working in a multidisciplinary approach. About twenty research topics have been divided in four research axes of which objectives are to develop a more sustainable agriculture. The platform has the ambition to discuss its results to a large comity gathering the actors of the agricultural development. The aim of our work is firstly to present Agrocology as a concept made of three interrelated aspects. To illustrate it, the organization and objectives of the research platform AgricultureIsLife will be discussed in a second part. [less ▲]

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See detailRhizobacterial volatile organic compounds implication in Brachypodium distachyon response to phosphorus deficiency
Baudson, Caroline ULg; Saunier de Cazenave-Mendaluk, Magdalena ULg; du Jardin, Patrick ULg et al

Poster (2014, February 07)

In agriculture, phosphorus (P) is considered as the second most growth-limiting macronutrient after nitrogen. However, P fertilizers are produced from non-renewable resources. In this context, sustainable ... [more ▼]

In agriculture, phosphorus (P) is considered as the second most growth-limiting macronutrient after nitrogen. However, P fertilizers are produced from non-renewable resources. In this context, sustainable production strategies have to be developed to enhance P use efficiency of crops, e.g. based on naturally occurring biotic interactions that limit the negative impacts of P deficiency in soils. Plant growth-promoting rhizobacteria (PGPR) have already revealed their ability to promote plant growth and tolerance to abiotic stresses through many mechanisms. Among them, the bacterial volatile organic compounds-mediated communication between plants and PGPR is still poorly documented. Our research project aims at studying the capacity of a model cereal plant (Brachypodium distachyon (L.) Beauv. Bd21) to face P deficiency in interaction with PGPR. The prerequisite of this project consists in characterizing Bd21 response to P deficiency by measuring plant biomass production and allocation, root system architecture, total phosphorus content, root-secreted and intracellular acid phosphatase activity under various P concentrations. Those results will allow us to define P-limiting conditions, in order to assess PGPR volatiles influence on plant response to P deficiency. This approach will use an ex-vitro co-cultivation system allowing volatiles-mediated interaction and should help us to unravel the ability of rhizobacterial volatiles to enhance plant tolerance to P deficiency. [less ▲]

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See detailUse of GC-MS in SIM-Scan mode for a selective quantification of polluting volatile organic compounds (VOC) in food industries
Eloundou Mballa, Pierre ULg; DETHIER, Bérénice ULg; Fauconnier, Marie-Laure ULg

Poster (2014, February 07)

The control of atmospheric emission discharges by food industries is classically achieved in two phases. The first one consists of sampling the target atmosphere followed by concentrating the volatile ... [more ▼]

The control of atmospheric emission discharges by food industries is classically achieved in two phases. The first one consists of sampling the target atmosphere followed by concentrating the volatile organic compounds (VOC) contained in a defined volume of this atmosphere on an adsorbing agent. These compounds are then thermally desorbed (TD) or desorbed with a solvent. In the second phase, the gas phase chromatogram (GC) will be coupled with mass spectrometry (MS) or flame ionization detector (FID) for compounds analysis. Quantitative analysis are usually achieved with solvent desorption followed by GC-FID. However, this mode imposes the dilution of the sample by a solvent and consequently may cause high detection thresholds as well as important matrix effect and possible interferences due to chemical reactions between analytes and the solvent. Besides, a preliminary qualitative analysis (GC-MS identification) is necessary. Combining TD-GC-MS allows very low detection thresholds to be reached, and the matrix effect and the interferences caused by products of the reactions between analytes and the solvent to be eliminated. The use of this combination in selected ion monitoring (SIM) mode allows targeting analytes via their characteristic ions, increasing their sensitivity and repeatability insuring more accuracy for simultaneous qualitative and quantitative analysis. This technique was experimented for seeking VOC in the atmosphere around food industries. [less ▲]

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See detailInteractions between new phenolic glycolipids and model membrane
Sainvitu, Pauline ULg; Nasir, Mehmet Nail ULg; Crowet, Jean-Marc ULg et al

Poster (2014, February 07)

Model membrane based on phospholipids (PL) layers are useful to mimic properties of plasma membranes. The interactions between new synthesized phenolic glycolipids (PGL) and biological membrane are ... [more ▼]

Model membrane based on phospholipids (PL) layers are useful to mimic properties of plasma membranes. The interactions between new synthesized phenolic glycolipids (PGL) and biological membrane are crucial to determine their potential as drug candidates and their cytotoxicity . [less ▲]

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See detailScreening of two agricultural genomic DNA libraries to seek new glycoside hydrolases
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2014, February 07)

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play an important role in the degradation of organic matter with enzymes able to degrade it. This ... [more ▼]

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play an important role in the degradation of organic matter with enzymes able to degrade it. This work aims to discover, by functional screening, new microbial glycoside hydrolases from soils collected in winter and spring in a winter wheat crop. The genomic DNA was extracted from both soils to construct two libraries in Escherichia coli. These libraries were then screened for beta-glucosidase activities on 2YT agar media containing 0.5% esculin and 0.1% ammonium iron (III) citrate. At this time, about 250.000 clones from each library have been screened. Two beta-glucosidases have already been found in the winter library while five beta-glucosidases and two glycosyltransferases were identified in the spring library. Sequence analyses with the BLASTX program revealed putative enzymes showing between 25% and 72% sequence identity with known enzymes and belonging to three glycoside hydrolase families (GH1, GH3 and GH20) and to two probably new glycosyltransferase families. Biochemical characterisation of the candidates at several pH values and temperatures, and with four substrates, is in progress. [less ▲]

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See detailIMPLEMENTATION OF A METAL STRUCTURED PACKING IN A FUNGAL BIOFILM REACTOR FOR THE PRODUCTION OF A RECOMBINANT PROTEIN BY ASPERGILLUS ORYZAE
Zune, Quentin ULg; Delepierre, Anissa; Toye, Dominique ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07)

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See detailBiodiversity and ecosystem services: think functional!
Uyttenbroeck, Roel ULg; Hatt, Séverin ULg; Paul, Aman ULg et al

Poster (2014, February 07)

During the last years, several studies and reviews have considered the relation between biodiversity and ecosystem functioning or the provision of ecosystem services. Many studies found that plant ... [more ▼]

During the last years, several studies and reviews have considered the relation between biodiversity and ecosystem functioning or the provision of ecosystem services. Many studies found that plant functional traits and plant functional diversity (FD) are key drivers in this relation in terrestrial ecosystems. Researchers used different methods to obtain a gradient in plant FD to examine the effect on ecosystem services, going from observational studies of natural communities to synthetic assemblages. Furthermore, different methods exist to quantify plant FD going from simple functional trait richness to indices, distance-based frameworks and the division into FD components. In the AgricultureIsLife project, we set up a field experiment aiming to examine the biodiversity – ecosystem service relation in agricultural context. The experiment consists of perennial wildflower strips with different plant functional diversities in an arable field with conventional crop production. The wildflower strips were sown as synthetic assemblages but are subject to natural succession during the following years. We monitor the evolution of FD from the sowing to the establishment of a typical wildflower strip using Rhao’s quadratic entropy index to quantify FD. In addition, the flower strips will be monitored for four ecosystem services they are expected to provide: pollination, pest control, biodiversity support and provision of valuable compounds. [less ▲]

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See detailAnalyzing relationships between cattle grazing behavior and pasture attributes using the inertial measurement unit of a mobile phone.
Andriamandroso, Andriamasinoro ULg; Lebeau, Frédéric ULg; Bindelle, Jérôme ULg

Poster (2014, February 07)

The recent technological developments are boosting the opportunities of accurate method to monitor resource use efficiency in agriculture and in their wake, precision livestock farming (PLF) has ... [more ▼]

The recent technological developments are boosting the opportunities of accurate method to monitor resource use efficiency in agriculture and in their wake, precision livestock farming (PLF) has experienced huge developments over the past decade. These developments focus on the optimization of individual performances of farm animals as opposed to herd management. The aim of this paper is to explore a method to detect accurately and to analyze changes in cattle's behaviors on pasture during grazing time using signals from the inertial measurement unit (IMU) of mobile devices as a possible tool to manage individual grazing behavior. Commercial iPhones or iPods, which include a 3-axis accelerometer, a gyroscope and a GPS sensor, are fitted on a halter and placed on the neck of grazing cows. The acquired IMU data are recovered using an open source application (Sensor Data, Wavefrontlabs) and analyzed in a “white-box” model of the cows’ movements. First results using time-domain analysis allowed the detection of grazing behaviors showed accuracies ranging between 84% and 96%, attesting the relevancy of the method. Refined signal processing method will improve the detection but will also inform more about the relative link between the behaviors and the pasture attributes such as sward height, composition and nutritive value. [less ▲]

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See detailRobot weed killers - no pain more gain
Krishna Moorthy Parvathi, Sruthi Moorthy ULg; Mercatoris, Benoît ULg; Boigelot, Bernard ULg

Poster (2014, February 07)

Weed destruction plays a significant role in crop production, and its automation has both economic and environmental benefits by minimizing the usage of chemicals in the fields. Our aim is to design a ... [more ▼]

Weed destruction plays a significant role in crop production, and its automation has both economic and environmental benefits by minimizing the usage of chemicals in the fields. Our aim is to design a small low-cost versatile robot allowing the destruction of weeds that lie between the crop rows by navigating in the field autonomously. Major challenges foreseen are: mapping the unknown geometry of the field, high-level planning of efficient and complete coverage of the field, and controlling the low-level operations of the robot. Traditionally, sensors like odometer have been used for localisation of robots but without much success in real-world scenarios. Specialized sensors like cameras will therefore be investigated and the plethora of image recognition algorithms will be explored and fine-tuned to enable Simultaneous Localisation And Mapping (SLAM) even on resource constrained robotic platforms. Vision-based localisation is not always viable because of the varying weather conditions of the environment and to overcome that, intelligent stochastic data fusion and machine learning algorithms will be utilized to combine data from heterogenous sensor. The image sensors for localisation will be re-used to differentiate crop rows from the weeds, which are cut when they grow. Finally, logics and reinforcement learning techniques will be explored, to exploit the generated map of the field and other sensorial information, to efficiently plan and execute weed elimination. [less ▲]

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See detailUse of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess
Brognaux, Alison ULg; Han, Shanshan; Sorensen, Soren et al

Poster (2014, February 07)

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See detailPaints based on renewable materials
Olive, Gilles ULg; Richel, Aurore ULg

in BOOK OF SHORT ABSTRACTS - POSTER PRESENTATIONS 19TH NATIONAL SYMPOSIUM ON APPLIED BIOLOGICAL SCIENCES (2014, February 07)

Paints, at the coating meaning, are known since a long time. Typically, paint is a dispersion of one or more powders (pigments and fillers) in a macromolecular substance ("resin"), called film-forming ... [more ▼]

Paints, at the coating meaning, are known since a long time. Typically, paint is a dispersion of one or more powders (pigments and fillers) in a macromolecular substance ("resin"), called film-forming material, diluted in solvents. Pigments are solid particles, used to give opacity and/or color. Today almost all pigments have a synthetic origin. The fillers are often natural compounds. Unlike pigments, fillers have low opacity and are colorless. These very cheap products achieve the required solid content under satisfactory economic conditions. Resins used in paints have the property of forming a continuous solid (hard or flexible) film under specific conditions. They are often called “resins”. They can be solid or liquid at room temperature. The solvents are used to control the viscosity of the paint in order to facilitate its production and application. The solvents used are volatile compounds. Additives are minor compounds (a few percent), whose function is to either promote or prevent some developments of the product. For example, surfactants improve the homogeneity of the dispersion. Some additives protect the film against mold or ultraviolet radiation, or against the formation of free radicals under the action of sunlight. Since the end of the 18th century most of those paints are petroleum-based. But the near disappearance of the petroleum causes a renewed interest in bio-based preparations. In this communication, we present an overview of bio-based alternatives. [less ▲]

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See detailFrom wood charcoals to trees: pitfalls and successes of the taxonomic identification in tropical contexts
Morin-Rivat, Julie ULg; De Weerdt, Joëlle; Hubau, Wannes et al

Poster (2014, February 07)

So as to document the past history of tropical forests, several palaeoenvironmental proxies have been used. For instance, charcoals from soil deposits provide a local signal of the evolution of the ... [more ▼]

So as to document the past history of tropical forests, several palaeoenvironmental proxies have been used. For instance, charcoals from soil deposits provide a local signal of the evolution of the vegetation together with snapshots of human interactions with the environment. As charcoal analyses are rare in tropical contexts, here we aim at presenting the different aspects of charcoal studies through their pitfalls and successes as well as the needs for further research. Charcoal analysis (anthracology) is a discipline initially from archaeobotany that consists in the analysis of pieces of charred wood primarily found in archaeological contexts but also in natural soil layers. Its goal is to identified the species that burnt during the past through the observation of the charred wood structure. Indeed carbonization, as the incomplete combustion of the ligneous material, preserves the wood structure. The identifications obtained through microscopic observations allow assessing past uses of wood and human impacts on the forest landscape. However, issues typically tropical exist: difficulties related to fieldwork accessibility, to sampling, to soil processing so as to collect the charcoals, difficulties related to the taxonomic identification because of the huge number of species and of the limited number of anatomical descriptions. New developments are nonetheless emerging for Central Africa with original anatomical descriptions, identification protocols and visual keys. [less ▲]

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See detailINFLUENCE OF STEAM EXPLOSION ON THECRYSTALLINITY OF CELLULOSE FIBER
Jacquet, Nicolas ULg; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

Poster (2014, February 07)

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct ... [more ▼]

The aim of the present study is to compare the effect of different steam explosion treatments on crystallinity properties of a pure bleached cellulose. Steam explosion process is composed of two distinct stages: vapocracking and explosive decompression. The treatment intensities is determined by a severity factor, established by a correlation between temperature process and retention time. The results show that steam explosion treatment has an impact on the crystallinity properties of pure cellulose fiber. When the severity factor is below 5.2, an increase of the overall crystallinity of the samples is observed with the treatment intensities. For higher intensities, a significant thermal degradation of cellulose lead to an important change in substrate composition, which lead to a further decrease of cellulose crystallinity. [less ▲]

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See detailPrerequisites for a Black locust genomic selection program
Verdu, Cindy ULg; Mengal, Coralie ULg; Henrotay, Frédéric ULg et al

Poster (2014, February 07)

The use of renewable resources as an alternative to fossil fuels has become a priority. Efficient use of forest as a resource for energy and green chemistry purposes require the development of suitable ... [more ▼]

The use of renewable resources as an alternative to fossil fuels has become a priority. Efficient use of forest as a resource for energy and green chemistry purposes require the development of suitable selected genotypes that are competitive and ready to meet the challenges of global change. In this context, the black locust, Robinia pseudoacacia L., is a very promising species which has many advantages in the context of current global change: high phenotypic plasticity, drought resistance, high biomass production and fixation of atmospheric nitrogen. The genetic improvement of woody species using traditional methods can take between 20 to 30 years. These times can be greatly reduced with the development of new selection methods such as genomic selection. Before starting a genomic selection program, it is necessary to 1) develop new molecular markers to achieve a very dense genetic map for genomic selection, 2) study the genetic diversity of the species present in Belgium and compare it with that of the native area, 3) study the structure and the relatedness of different populations, 4) establish a core collection gathering the most genetically diverse individuals, and 5) as black locust is an exotic species, verify its invasiveness in Belgium. These 5 steps will be detailed and the first results obtained will be discussed. [less ▲]

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See detailContribution to the study of alliinase, the active principle of garlic
DETHIER, Bérénice ULg; Ponchaux, Julien; Laloux, Morgan et al

Poster (2014, February 07)

Alliinase is a crucial enzyme in the Allium genus. The conversion of its substrates, cysteine sulfoxides, into volatile thiosulfinates is an important mechanism in the defence of the plant. It also ... [more ▼]

Alliinase is a crucial enzyme in the Allium genus. The conversion of its substrates, cysteine sulfoxides, into volatile thiosulfinates is an important mechanism in the defence of the plant. It also provides the typical pungent flavour of garlic. As a matter of fact, the thiosulfinates decompose in a range of organosulfur compounds (OSC) known for their biological activities (antioxidant, anticancer, anti-diabetes properties, etc.). The environment of the decomposition of the thiosulfinates determines the type and amount of products released. A deeper knowledge of the conditions that form each OSC could help us in the preparation of garlic-based nutraceutics in the perspective of healthier food consumption. This study aims to improve the extraction and purification the enzyme alliinase in order to perform the reaction between the enzyme and its substrates and assess the potentialities of garlic preparations. Three methods have been applied to evaluate the efficiency of the extractions and purifications of the enzyme. A first idea of the purity of the enzyme is given by an electrophoresis separation of each sample on a polyacrylamide gel (SDS-PAGE). The protein content is then measured by UV-vis spectrometry with Lowry-Folin reagent for the coloration and BSA as an external standard. Finally, the specific activity is assessed by an indirect measure of the pyruvate (released as a co-product): the addition of NADH and lactate dehydrogenase turns the pyruvate in lactate, and the disappearance of NADH is measured by UV-vis spectrometry at 340 nm. The extraction of the enzyme from garlic was performed either by PEG 8000 precipitation or by ammonium sulphate precipitation. Two purifications were tested: affinity chromatography (on ConA) and size-exclusion chromatography. The combination of the ammonium sulphate process with the ConA chromatography provided the purest enzyme, with the best activity but a lower yield than the size-exclusion process. Finally the stability of the enzyme has been assessed at 4, -20 and -80 °C, showing that the enzyme could be kept at -80 °C for over 4 months without deterioration, while activity loss was observed at higher temperature. [less ▲]

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See detailIntegrated farming systems in Kinshasa (DRC) Diversity of agricultural practices
Mafwila Kinkela, Patrick ULg; Bindelle, Jérôme ULg; WILLEMS, E, Emilie et al

Poster (2014, February 07)

In Kinshasa in the Democratic Republic of Congo, integrated farming of livestock and fish farming is little documented while it is an interesting way of ecological intensification systems. After ... [more ▼]

In Kinshasa in the Democratic Republic of Congo, integrated farming of livestock and fish farming is little documented while it is an interesting way of ecological intensification systems. After identifying the density of fish ponds in the territory of the city using satellite images, about 200 farms with at least one pond and located in 2 in peri-urban and rural areas were surveyed to characterize the practical integration of the agricultural system. The preliminary results in one of the valleys indicate that the practice of agriculture in synergy with the pig and fish farming is a common practice although flow of components are still insufficiently integrated and some of them are not optimized . The fish - pig farming associations with or without vegetable production are 44 % of the sample. The identification of flow on farms showed that the manure of pigs is mainly used for crops (51%) or sold (28%), while a small part is used to fertilize the ponds. This may be explained by the predominant share of income (67%) devoted to vegetable production while sludge of ponds is rarely used to fertilize area of vegetable production. Residues of vegetable crops are rarely used to feed pigs and fish because of their limited availability. Feeding pigs and fish is based primarily on agro-industrial by-products such as wheat bran (81%) and the spent brewery grains (62%) and forages (62%). The presentation will analyze the results obtained in the three sites visited and outline opportunities for improvement. [less ▲]

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See detailPhysico-chemical properties and aroma profile of Acacia Honey produced in Romania
Madas, Mariana-Niculina ULg; Francis, Frédéric ULg; Marghitas, L.A. et al

in Communications in Agricultural and Applied Biological Sciences (2014, February 07), 79(1), 133-135

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See detailEfficient computation of genomically-enhanced inbreeding coefficients
Faux, Pierre ULg; Gengler, Nicolas ULg

Poster (2014, February 07)

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See detailCan phytoestrogen-rich plants restore the image of livestock in terms of human health? Do they promise a differentiated quality products chain?
Daems, Frédéric ULg; Jasselette, Christophe; Lognay, Georges ULg et al

Poster (2014, February 07)

In a project named PhytoHealth, the development of analytical methods for studying the impact of phytoestrogens rich diet on the «health value» of animal products is in progress. Despite the ambiguous ... [more ▼]

In a project named PhytoHealth, the development of analytical methods for studying the impact of phytoestrogens rich diet on the «health value» of animal products is in progress. Despite the ambiguous image that have phytoestrogens, some of their metabolites seemed to have potentially beneficial effects to human health. In a first time, a microbial metabolite (equol) was selected and its metabolism in dairy cow is studied. A first method using the UPLC®-MS/MS technology has been validated and has achieved a screening of equol content in milk consumed in Wallonia. Equol was found in all milk samples analyzed and a significant difference between farming methods has been highlighted. A second analytical method to quantify the equol precursors was then developed and a study of forage plants consumed by Belgian dairy cows will be conducted to select the richest fodder varieties. Other methods will be developed to better understand the metabolism in the cow and estimate the impact of enriched milk on human health. An original approach involving the use of minipigs will be considered. In the end, creating of a differentiated quality animal products chain could be interesting for the consumer, but also recoverable for the producer. [less ▲]

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See detailAllelopathic potential of sunflower against the great brome
Bouhaouel, Imen ULg; Gfeller, Aurélie; Fauconnier, Marie-Laure ULg et al

Poster (2014, February 06)

Control methods commonly used to suppress the great brome (Bromus diandrus Roth., syn. Bromus rigidus Roth. subsp. gussonii Parl.) in Tunisian cereal crop are essentially chemical, raising both efficacy ... [more ▼]

Control methods commonly used to suppress the great brome (Bromus diandrus Roth., syn. Bromus rigidus Roth. subsp. gussonii Parl.) in Tunisian cereal crop are essentially chemical, raising both efficacy and safety issues. The introduction of allelopathic species into the crop rotation or utilizing allelopathic plants as living/green mulches has been suggested as a cost-effective way to reduce the weed presence. Among these species, the sunflower (Helianthus annuus L.) has shown an allelopathic potential against some troublesome weed species. In this study, we analyzed the biological activities of water extract of different tissues (root, shoot, leaf and flower) of sunflower on the seedling establishment of the great brome. In a second experiment, the allelopathic influence of sunflower residues (leaf or flower) against this weed was also studied under glasshouse conditions at more advanced stages of growth using different concentrations (0, 6, 12 and 18g tissue dry weight / kg of soil). The first experiment showed an effect depending on the parts of the sunflower. Indeed, the roots seem to be the less allelopathic part (22% of root inhibition growth) as compared to the leaves and flowers (82% and 100%, respectively). This potential seems to simultaneously affect the radicle and the coleoptile growth of the great brome. In the second experiment, weed growth was inhibited in a dose-dependent manner, using increasing amounts of sunflower residues. The allelopathic potential of the leaves or flowers reduced both the root or shoot length and biomass accumulation of the weed. These results suggest that the sunflower can be a good previous crop for cereal cultivation by controlling the presence of some weeds, including the great brome. In this perspective, the inhibitory effects of sunflower residues on cultivated cereals in the field need to be assessed. [less ▲]

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See detailForecasting Daily Solar Energy Production Using Robust Regression Techniques
Louppe, Gilles ULg; Prettenhofer, Peter

Conference (2014, February 05)

We describe a novel approach to forecast daily solar energy production based on the output of a numerical weather prediction (NWP) model using non-parametric robust regression techniques. Our approach ... [more ▼]

We describe a novel approach to forecast daily solar energy production based on the output of a numerical weather prediction (NWP) model using non-parametric robust regression techniques. Our approach comprises two steps: First, we use a non-linear interpolation technique, Gaussian Process regression (also known as Kriging in Geostatistics), to interpolate the coarse NWP grid to the location of the solar energy production facilities. Second, we use Gradient Boosted Regression Trees, a non-parametric regression technique, to predict the daily solar energy output based on the interpolated NWP model and additional spatio-temporal features. Experimental evidence suggests that two aspects of our approach are crucial for its effectiveness: a) the ability of Gaussian Process regression to incorporate both input and output uncertainty which we leverage by deriving input uncertainty from an ensemble of 11 NWP models and including convidence intervals alongside the interpolated point estimates and b) the ability of Gradient Boosted Regression Trees to handle outliers in the outputs by using robust loss functions - a property that is very important due to the volatile nature of solar energy output. We evaluated the approach on a dataset of daily solar energy measurements from 98 stations in Oklahoma. The results show a relative improvement of 17.17% and 46.19% over the baselines, Spline Interpolation and Gaussian Mixture Models, resp. [less ▲]

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See detailPaleoenvironmental record of the Amik Basin (Amuq Plain, Southern Turkey) over the last 4000 years
Hubert, Aurelia ULg; El Ouahabi, Meriam ULg; Lebeau, Hélène ULg et al

Poster (2014, February 04)

Continous human occupation is attested in the Amik Basin since 6000-7000 BC. The low-lying Amuq plain is covered by tell settlements first explored by Robert Braidwood in the 1930s. The Basin also is ... [more ▼]

Continous human occupation is attested in the Amik Basin since 6000-7000 BC. The low-lying Amuq plain is covered by tell settlements first explored by Robert Braidwood in the 1930s. The Basin also is crossed by The Dead Sea Fault (DSF), a major neotectonic structure in the Middle East extending from the Red Sea in the south to Turkey in the north. The study focuses on the sedimentary record of the Amik Lake occupying the central part of the Basin. Our objective is to constrain major paleo-environmental changes in the area over the last 4000 years and assess possible human impact. The lake has been drained and progressively dried up since the mid-50s so that it is not watered during the summer season and constitutes a unique opportunity to collect sediment records. Sediments were collected at 1 cm to 2 cm intervals in a trench and in cores up to a depth of 5 meters in the clay deposits. A diverse array of complementary methods is applied to study the records: magnetic susceptibility, grain size, organic matter and inorganic carbon (L.O.I), XRD mineralogy, XRF geochemistry, carbon geochemistry. The age of the record is constrained combining radionuclide and radiocarbon dating. The record shows two intense phases of soil erosion with enrichments in Chromium and Nickel. The most recent erosion phase might be linked with enhanced development during the Roman and the growth of the Antioch City. The oldest one would occur around 3000 BC. The record also allows reconstructing past lake level variations and discusses the results in comparison with variations of the Dead Sea. [less ▲]

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See detailLes représentations de Synagoga aux portails occidentaux de la cathédrale d’Amiens : l’illustration de l’ « antijudaïsme » au lendemain de Latran IV ?
Lekane, Marie ULg

Conference (2014, February 04)

Les portails occidentaux amiénois sont munis de trois représentations de la Synagogue, sous la forme bien connue d’une personnification féminine aux yeux bandés. Deux scènes particulières, montrant la ... [more ▼]

Les portails occidentaux amiénois sont munis de trois représentations de la Synagogue, sous la forme bien connue d’une personnification féminine aux yeux bandés. Deux scènes particulières, montrant la Synagoga, se jouent sur des culots du portail de la Vierge, sous les pieds des Rois mages. Si l’une de ces scènes semble représenter le découronnement de la figure allégorique par le Christ, la seconde est plus énigmatique. Au portail du Jugement dernier, sous les pieds de l’archange Michel, se loge le couple antagoniste Ecclesia-Synagoga. La position éminemment négative de la Synagogue – sous le plateau démoniaque, à senestre du Christ, côtoyant le monde infernal – fait-elle écho aux récentes positions contre les Juifs prises et actées lors du Concile de Latran IV (1215) ? Afin de pouvoir amener des pistes de réflexions sur ces points, déjà soulevés par certains auteurs (W. Schlink, 1991), nous interrogerons les sources historiques sur la présence effective d’une communauté juive à Amiens et sur leurs conditions de vie. En outre, et plus généralement, nous analyserons les retombées tangibles des décisions prises lors du Concile de 1215, auquel participait Jean d’Abbeville, doyen de la cathédrale d’Amiens au moment de sa reconstruction, volontiers présenté comme « programmateur » de l’ensemble. Il est indispensable de reconsidérer soigneusement ces images et leurs composantes afin de définir leurs particularités et d’infirmer ou confirmer un « antijudaïsme » aux portails amiénois, ou du moins de pouvoir le caractériser. [less ▲]

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See detailCoMBiSpeC: A novel approach in the modelling of massive binaries
Palate, Matthieu ULg

in EAS Publications Series (2014, February 01)

Spectral modelling and especially for massive stars is not a straightforward problem and over the past decades many advances have been made in this field. We now have sophisticated model atmosphere codes ... [more ▼]

Spectral modelling and especially for massive stars is not a straightforward problem and over the past decades many advances have been made in this field. We now have sophisticated model atmosphere codes that allow to derive the stellar parameters. However, these codes are based on the assumption that the star is single and spherical which is no longer valid for the components of a binary system. On the other hand, observational studies indicate that binarity has an impact on the spectra. This is why we have developed a model called CoMBiSpeC (code of massive binary spectral computation) that specifically accounts for the impact of binarity on the spectra. [less ▲]

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See detailImportance of recent extreme weather variation in Djibouti and need for impact quantification
Nour Ayeh, Moustapha; Mahamoud, Ayan; Saad, Osman et al

Poster (2014, February)

This analysis shows that the current rainfall deficit is exceptional and historically unique. The significant population migration induced by the drought to Djibouti city must be supervised, especially ... [more ▼]

This analysis shows that the current rainfall deficit is exceptional and historically unique. The significant population migration induced by the drought to Djibouti city must be supervised, especially during their spontaneous settling. This presented example confirms that current rainfall shortages and increasing temperature extremes are impactinglocal people who urgently need adaptation and DRR strategies. It is necessary to reduce exposure to hydrological risks of these affected populations, in order that victims of the drought are not carried away by a rainfall excess. [less ▲]

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See detailBacterial and viral pneumonia
Clercx, Cécile ULg

in Proceedings 35 Internationaler Fortbildungskurs Kleintierkrankheiten (2014, February)

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See detailIMPACT OF COVER CROP MANAGEMENT ON CROP PRODUCTION: A FIELD EXPERIMENT IN WALLONIA CONTEXT
Hiel, Marie-Pierre ULg; Bodson, Bernard ULg

Poster (2014, February)

Minimal soil tillage methods, crop rotation, cover crop and continuous plant residue cover are the main pillars of sustainable agriculture. Lower energy consumption, costs and time are some direct ... [more ▼]

Minimal soil tillage methods, crop rotation, cover crop and continuous plant residue cover are the main pillars of sustainable agriculture. Lower energy consumption, costs and time are some direct benefits in favor to the adaptation of this agriculture. This practices aims also directly at protecting the soil from wind and water erosion by covering the soil. Lower disruption of the soil aims at developping the micro- and macro-fauna activity that increases soil fertility and carbon and nitrogen sequestration in soils. Unfortunately, some of the great constraints to the adaptation of conservation agriculture remain weed management, fungal diseases and pest management, that has been shown to be a problem in non-ploughed fields. The aim of our study is to evaluate, on a same field (Wallonia context), contrasted tillage methods for managing the cover crop and the implantation of the main crop. The following measurements were taken: germination rate, root and shoot biomass development, root notation (size and shape), leaf area index and quality of harvested product. Preliminary results indicate that the different tillage methods did not have a significant impact on crop production or development. However changes in germination dynamics were observed, the reduced tillage inducing slower germination. Regarding weeds populations, some difference were observed in weed occurrences. Since a field exp [less ▲]

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See detailAnthropogenic effects on African Landscapes: Spatial Structure, Typologies, Ecological Impact
Vranken, Isabelle ULg

Poster (2014, February)

The problematique of anthropogenic disturbance on African landscapes is studied within the double framework of DPSIR (Drivers Pressures States Impact Response) and Pattern/Process Paradygm (Landscape ... [more ▼]

The problematique of anthropogenic disturbance on African landscapes is studied within the double framework of DPSIR (Drivers Pressures States Impact Response) and Pattern/Process Paradygm (Landscape Ecology). 20 landscae images from D.R. Congo, Ivory Coast and Benin were studied using indexes of spatial (compositional and configurational) heterogeneity and anthropogenic disturbance in order to highlight the relationship between spatial structure and disturbance intensity. The results show maximal heterogeneity at intermediate anthropogenic disturbances and fasten the triangular relationship between anthropogenic effect, biodiversity and spatial heterogeneity, that had been partially theoretised with the intermidiate disturbance and habitat heterogeneity hypotheses. [less ▲]

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See detailOrigin and Pulsation of Hot Subdwarfs
Randall, Suzanna K; Fontaine, Gilles; Charpinet, Stéphane et al

in Proceedings of the International Astronomical Union (2014, February), 301

We briefly introduce hot subdwarfs and their evolutionary status before discussing the different types of known pulsators in more detail. Currently, at least six apparently distinct types of variable are ... [more ▼]

We briefly introduce hot subdwarfs and their evolutionary status before discussing the different types of known pulsators in more detail. Currently, at least six apparently distinct types of variable are known among hot subdwarfs, encompassing p- as well as g-mode pulsators and objects in the Galactic field as well as in globular clusters. Most of the oscillations detected can be explained in terms of an iron opacity mechanism, and quantitative asteroseismology has been very successful for some of the pulsators. In addition to helping constrain possible evolutionary scenarios, studies focussing on stellar pulsations have also been used to infer planets and characterize the rotation of the host star. [less ▲]

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See detailCorrelations of the High Resolution MRI aspect of GH-secreting pituitary adenomas prior to treatment
Potorac, Iulia ULg; PETROSSIANS, Patrick ULg; Schillo, F et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailClinical characteristics of patients with AIP mutation-related prolactinomas
Camby, S; Daly, Adrian ULg; VROONEN, Laurent ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailLiege acromegay Survey : An epidemiological study about the average age of death and the causes of mortality of acromegalic patients managed in liege
Petignot, S; PETROSSIANS, Patrick ULg; Daly, Adrian ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailEffective stress method to be used in beam finite elements to take local instabilities into account
Franssen, Jean-Marc ULg; Cowez, Baptiste; Gernay, Thomas ULg

in Proceedings of the 11th IAFSS Symposium (2014, February)

In the fire situation, Bernoulli beam finite elements are the workhorse used in numerical calculation model for simulating the behaviour of the structure. Such finite elements treat all sections as class ... [more ▼]

In the fire situation, Bernoulli beam finite elements are the workhorse used in numerical calculation model for simulating the behaviour of the structure. Such finite elements treat all sections as class 1 (stocky) sections whatever the slenderness of the plates that make the section, allowing the development of a full plastic stress distribution in the section which leads to complete plastic redistribution along the members in the structure. This type of element is thus not adapted for modeling structures that contain slender sections of class 2, 3 or 4. This document presents a new approach to take into account local instabilities in slender sections using beam finite elements. The new approach is based on an effective constitutive law of steel. The effective law is not symmetrical with respect to tension and compression because, in tension, the stress-strain relationship is not modified whereas, in compression, the stress-strain relationship is modified. [less ▲]

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See detailNew alternatives to chemical pesticides: deciphering the action mechanisms of lipid based plant elicitors via complementary biophysical and biological approaches.
Nasir, Mehmet Nail ULg; Polo Lozano, Damien ULg; Luzuriaga Loaiza, Walter ULg et al

Poster (2014, February)

Nowadays, many health and environmental problems are caused by the use of chemical pesticides. In this context, an increasing demand for alternative products such as biopesticides has been observed. Among ... [more ▼]

Nowadays, many health and environmental problems are caused by the use of chemical pesticides. In this context, an increasing demand for alternative products such as biopesticides has been observed. Among biopesticides, elicitor molecules which are able to trigger immune defense responses in plants are one of the most promising options. Although numerous elicitors have been discovered, the mechanisms involved in the perception, by plants, of only a few molecules have been identified. These elicitors usually interact with proteic receptors but we have recently shown that they may also act on the lipid phase of the plasma membrane. This project first aims to improve our understanding of the molecular mechanisms involved in the recognition of specific lipid based elicitors (LBE). On that basis, the FIELD project will contribute to the design and the development of innovative compounds derived natural LBE. A multi-disciplinary approach, based on chemistry, bio-physics, bio-chemistry, and phytopathology will be followed by a consortium of different research groups from Gembloux Agro-Bio Tech in close collaboration with teams from foreign institutions. [less ▲]

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See detailSubspace and nonlinear-normal-modes-based identification of a beam with softening-hardening behaviour
Grappasonni, Chiara ULg; Noël, Jean-Philippe ULg; Kerschen, Gaëtan ULg

in Proceedings of the International Modal Analysis Conference (IMAC) XXXII (2014, February)

The capability to reproduce and predict with high accuracy the behaviour of a real system is a fundamental task of numerical models. In nonlinear structural dynamics, additional parameters compared to ... [more ▼]

The capability to reproduce and predict with high accuracy the behaviour of a real system is a fundamental task of numerical models. In nonlinear structural dynamics, additional parameters compared to classical linear modelling, which include the nonlinear coefficient and the mathematical form of the nonlinearity, need to be identified to bring the numerical predictions in good agreement with the experimental observations. In this context, the present paper presents a method for the identification of an experimental cantilever beam with a geometrically nonlinear thin beam clamped with a prestress, hence giving rise to a softening-hardening nonlinearity. A novel nonlinear subspace identification method formulated in the frequency domain is first exploited to estimate the nonlinear parameters of the real structure together with the underlying linear system directly from the experimental tests. Then a finite element model, built from the estimated parameters, is used to compute the backbone of the first nonlinear normal mode motion. These numerical evaluations are compared to a nonlinear normal modes-based identification of the structure using system responses to stepped sine excitation at different forcing levels. [less ▲]

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See detailBronchiectasis
Clercx, Cécile ULg

in Proceedings 35 Internationaler Fortbildungskurs Kleintierkrankheiten (2014, February)

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See detailG-mode trapping and period spacings in hot B subdwarf stars
Charpinet, Stéphane; Van Grootel, Valérie ULg; Brassard, Pierre et al

in Proceedings of the International Astronomical Union (2014, February), 301

Hot B subdwarfs (sdB) are hot and compact helium core burning stars of nearly half a solar mass that can develop pulsational instabilities driving acoustic and/or gravity modes. These evolved stars are ... [more ▼]

Hot B subdwarfs (sdB) are hot and compact helium core burning stars of nearly half a solar mass that can develop pulsational instabilities driving acoustic and/or gravity modes. These evolved stars are expected to be chemically stratified with an almost pure hydrogen envelope surrounding a helium mantle on top of a carbon/oxygen enriched core. However, the sdB stars pulsating in g-modes show regularities in their observed period distributions that, surprisingly (at first sight), are typical of the behavior of high order g-modes in chemically homogeneous (i.e., non-stratified) stars. This led to a claim that hot B subdwarfs could be much less chemically stratified than previously thought. Here, we reinvestigate trapping effects affecting g-modes in sdB stars. We show that standard stratified models of such stars can also produce nearly constant period spacings in the low frequency range similar to those found in g-mode spectra of sdB stars monitored with Kepler. [less ▲]

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See detailLe PPNAD, une cause rare de syndrome de Cushing
Petignot, S; VROONEN, Laurent ULg; HAMOIR, Etienne ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailLong fibrosis
Clercx, Cécile ULg

in proceedings 35 Internationaler Fortbildungskurs Kleintierkrankheiten (2014, February)

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See detailPulsations in white dwarf stars
Fontaine, Gilles; Bergeron, Pierre; Brassard, Pierre et al

in Proceedings of the International Astronomical Union (2014, February), 301

We first present a brief description of the six distinct families of pulsating white dwarfs that are now known. These are all opacity-driven pulsators showing low- to mid-order, low-degree gravity modes ... [more ▼]

We first present a brief description of the six distinct families of pulsating white dwarfs that are now known. These are all opacity-driven pulsators showing low- to mid-order, low-degree gravity modes. We then discuss some recent highlights that have come up in the field of white dwarf asteroseismology. [less ▲]

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See detailMicrofluidic on optical fibers: Towards a new kind of fluorescent biosensor
Lismont, Marjorie ULg; Vandewalle, Nicolas ULg; Weyer, Floriane ULg et al

Poster (2014, February)

In recent works, the behavior of droplets moving along vertical treads due to gravity was studied. It appeared that the droplet can be stopped by encountering a horizontal fiber depending on droplet ... [more ▼]

In recent works, the behavior of droplets moving along vertical treads due to gravity was studied. It appeared that the droplet can be stopped by encountering a horizontal fiber depending on droplet volumes and fiber characteristics. On the basis of this behavior and by replacing treads by two crossed optical fibers, it is possible to combine fluidics and optics to develop a new kind of fluorescent sensor. In our work, the intersection between two crossed optical fibers is used as the basic unit of an original optofluidic biosensor. These two optical fibers are used as droplets carriers: one for probe molecules and the other one for target species. The fiber's junction catches the droplets and act as a reaction center. The main advantage of using optical fibers resides in their ability to propagate and collect light to and from the droplet localized at the fiber's crossing. This optical fiber configuration can therefore allow the study of biological interactions using fluorescent labels. This new and versatile detection scheme was validated on a calcium indicator where ions detection is accomplished by using a dye, Oregon green Bapta-2, that has a Ca 2+ recognition group as well as an entity exhibiting fluorescence. A FRET recognition event, between Rh-Con A and FITC-Dextran, was also investigated to detect glucose. Finally, a prototype of a multiplexing device, composed of several juxtaposed fibers' junctions, was developed. [less ▲]

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See detailChronische Bronchitis Hund
Clercx, Cécile ULg

in Proceedings 35 Internationaler Fortbildungskurs Kleintierkrankheiten (2014, February)

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See detail7. Lutte intégrée contre les ravageurs
Chavalle, Sandrine; Censier, Florence ULg; Jacquemin, Guillaume et al

in Destain, Jean-Pierre; Bodson, Bernard (Eds.) Livre Blanc Céréales (2014, February)

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See detailMetal Enhanced Fluorescence: effect of surface coating
Lismont, Marjorie ULg; François, Alexandre; Dreesen, Laurent ULg et al

in SPIE proceeding (2014, February)

Among the emerging treatments for cancer, Photodynamic Therapy (PDT) is thought to be one of the most promising. PDT uses light sensitive molecules, or photosensitizer, to produce, under specific ... [more ▼]

Among the emerging treatments for cancer, Photodynamic Therapy (PDT) is thought to be one of the most promising. PDT uses light sensitive molecules, or photosensitizer, to produce, under specific irradiation, toxic reactive oxygen species (ROS) to kill cancer cells. However, the amount of ROS generated is limited by both the fluorescence lifetime of the photosensitizer and its concentration around the cancer cells. Metal Enhanced Fluorescence (MEF), a phenomenon arising when a fluorophore is in closed proximity to a metallic structure such as metallic films or nanostructures, is seen as a way to solve these problems by reducing the fluorescence lifetime and increasing the fluorescence emission of the fluorophore. Protoporphyrin IX (PpIX) is a commonly used photosensitizer to treat skin cancers, which presents an intense absorption band around 400 nm while emitting around 630 nm. Because silver nanoparticles (Ag NPs) exhibit a strong Localized Surface Plasmon Resonance (LSPR) around 400 nm, MEF of the PpIX is expected when immobilized onto Ag NPs. Here, we investigate the relevant parameters influencing the coupling effects between the LSPR in Ag NPs and PpIX attached onto the Ag NPs surface when the Ag NPs are dispersed in solution or electrostatically bound to a glass slide. In particular, we study the distance-dependent of MEF by applying multiple layers of polyelectrolyte to progressively increase the distance between Ag NPs and PpIX, covalently bond to the last polyelectrolyte layer as well as exploring the use of Ag NPs of different sizes ranging from 40 to 100 nm. [less ▲]

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See detailHypopituitarism in pituitary gigantism (results of an international study)
Rostomyan, L; Daly, Adrian ULg; Shah, N et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailA giant treated with growth hormone
Rostomyan, Liliya ULg; Potorac, Iulia ULg; Daly, Adrian ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailIdentification of a Time-varying Beam Using Hilbert Vibration Decomposition
Bertha, Mathieu ULg; Golinval, Jean-Claude ULg

in Proceedings of the International Modal Analysis Conference (IMAC) XXXII (2014, February)

The present work is concerned by modal identification of time-varying systems. For this purpose, a method based on instantaneous frequency identification and synchronous demodulation is used to extract ... [more ▼]

The present work is concerned by modal identification of time-varying systems. For this purpose, a method based on instantaneous frequency identification and synchronous demodulation is used to extract modal components from recorded signals. The proposed method of iterated sifting process is based on the Hilbert Vibration Decomposition (HVD) technique which is used to extract the instantaneous dominant vibrating component at each iteration. A source separation preprocessing step is introduced to treat multiple degree-of-freedom systems in an optimal way. Sources are used as reference signals to get a single instantaneous frequency of each mode for the demodulation on all the channels. The algorithm is presented and is applied to numerical simulation of a randomly excited time-varying structure for illustration purpose. The investigated structure is made up of a beam on which a non-negligible mass is traveling. The variable location of the mass results in changes in modal parameters. [less ▲]

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See detailLes modèles économiques du bioraffinage en région wallonne : de l'exploration des débouchés et des niches de marché aux stratégies de développement industriel
Richel, Aurore ULg

Conference (2014, February)

1) Acteurs de la bio-économie en Wallonie 2 Transition vers le bioraffinage de seconde génération ? Les grandes questions et éléments de réponse (Flux de matières premières ?, Technologies de conversion ... [more ▼]

1) Acteurs de la bio-économie en Wallonie 2 Transition vers le bioraffinage de seconde génération ? Les grandes questions et éléments de réponse (Flux de matières premières ?, Technologies de conversion ? Produits cibles et marchés ? Positionnement des acteurs académiques et privés sur la chaine de valeur ? 3) Flux de matières premières en Wallonie – Carte d’occupation des sols, inventaire du potentiel en biomasse pour le bioraffinage de seconde génération (biomasse agricole, forestière, coproduits des industries de transformation food et non-food) 4) Quel(s) produit(s) et quel(s) marché(s) ? Liste des 10 molécules d’intérêt pour la Wallonie, molécules à haute valeur ajoutée et marchés de niche, cibles actuelles des recherches académiques et industrielles 5) Stratégie wallonne en termes de bioraffinage de seconde génération – quelques exemples d’initiatives R&D privées et publiques 6) Conclusions [less ▲]

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See detailEvaluation of the Response of Shear Critical Walls Using a Three-Parameter Kinematic Theory
Mihaylov, Boyan ULg; Hannewald, Pia; Beyer, Katrin

in The 4th International fib Congress 2014 Mumbai - Proceedings (2014, February)

This paper discusses a newly developed three-parameter kinematic theory (3PKT) for shear critical walls with the help of four wall tests. The 3PKT is used to predict the pre- and post- peak response of ... [more ▼]

This paper discusses a newly developed three-parameter kinematic theory (3PKT) for shear critical walls with the help of four wall tests. The 3PKT is used to predict the pre- and post- peak response of the test units. Comparisons are performed with finite element (FE) models and plastic hinge models in combination with a shear degradation model. It is found that the latter underestimate the displacement capacity of the walls while the former do not predict well the post-peak response. The 3PKT with only three degrees of freedom captures the complete response of the walls provided that the size of the critical loading zone (CLZ) of the wall is well predicted. [less ▲]

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See detailExploration on structure activity relation of natural, self-assembling cyclic lipodepsipeptides
Geudens, Niels; Feher, Kristina; De Vleeschouwer, Matthias et al

Poster (2014, February)

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See detailThe harmonic balance method for advanced analysis and design of nonlinear mechanical systems
Detroux, Thibaut ULg; Renson, Ludovic ULg; Kerschen, Gaëtan ULg

in Proceedings of the SEM IMAC XXXII (2014, February)

As a tool for analyzing nonlinear large-scale structures, the harmonic balance (HB) method has recently received increasing attention in the structural dynamics community. However, its use was so far ... [more ▼]

As a tool for analyzing nonlinear large-scale structures, the harmonic balance (HB) method has recently received increasing attention in the structural dynamics community. However, its use was so far limited to the approximation and study of periodic solutions, and other methods as the shooting and orthogonal collocation techniques were usually preferred to further analyze these solutions and to study their bifurcations. This is why the present paper intends to demonstrate how one can take advantage of the HB method as an efficient alternative to the cited techniques. Two different applications are studied, namely the normal modes of a spacecraft and the optimization of the design of a vibration absorber. The interesting filtering feature of the HB method and the implementation of an efficient bifurcation tracking extension are illustrated. [less ▲]

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See detailReceptor expression in craniopharyngiomas causing tumor growth in pregnancy : case report and review of the literature
VROONEN, Laurent ULg; Tome Garcia, M; THIRY, Albert ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailNovel fibroblast growth factor receptor 1 mutation causing normosmic idiopathic hypogonadotropic hypogonadism
Chachati, AS; Potorac, I; DEBRAY, François-Guillaume ULg et al

in Abstract book - Symposium "Perspectives in Endocrinology" Congresses Highlights 2013:ECE Copenhagen, ENDO SF, SFE Paris (2014, February)

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See detailA stochastic framework for subspace identification of a strongly nonlinear aerospace structure
Noël, Jean-Philippe ULg; Schoukens, Johan; Kerschen, Gaëtan ULg

in Proceedings of the International Modal Analysis Conference (IMAC) XXXII (2014, February)

The present study exploits the maximum likelihood identification framework for deriving statistically-optimal models of nonlinear mechanical systems. The identification problem is formulated in the ... [more ▼]

The present study exploits the maximum likelihood identification framework for deriving statistically-optimal models of nonlinear mechanical systems. The identification problem is formulated in the frequency domain, and model parameters are calculated by minimising a weighted least-squares cost function. Initial values of the model parameters are obtained by means of a nonlinear subspace algorithm. The complete identification methodology is first demonstrated on a Duffing oscillator, prior to being applied to a full-scale aerospace structure. [less ▲]

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See detailCan a global dynamic vegetation model be used for both grassland and crop modeling at the local scale?
Minet, Julien ULg; Tychon, Bernard ULg; Jacquemin, Ingrid ULg et al

Poster (2014, February)

We report on the use of a dynamic vegetation model, CARAIB, within two modeling exercises in the framework of MACSUR. CARAIB is a physically-based, mechanistic model that calculates the carbon ... [more ▼]

We report on the use of a dynamic vegetation model, CARAIB, within two modeling exercises in the framework of MACSUR. CARAIB is a physically-based, mechanistic model that calculates the carbon assimilation of the vegetation as a function of the soil and climatic conditions. Within MACSUR, it was used in the model intercomparison exercises for grassland and crop modeling, in the LiveM 2.4 and CropM WP4 tasks, respectively. For grassland modeling, blind model runs at 11 locations were performed for various time ranges (few years). For crop modeling, a sensitivity analysis for building impact response surfaces (IRS) was performed, based on a bench of model runs at different levels of perturbation in the temperature and precipitation input data over 30 years. For grassland modeling, specific management functions accounting for the cutting or grazing of the grass were added to the model, in the framework of the MACSUR intercomparison. Initially developed for modeling the carbon dynamics of the natural vegetation, CARAIB was already adapted for crop modeling but further modifications regarding the management, i.e., yearly-dependent sowing dates, were introduced. For grassland modeling, simulation results will be further intercompared with other modeling groups, but preliminary results showed that the model could cope with the introduction of the grass cutting module. For crop modeling, building the IRS over 30 years permitted to assess the sensitivity of the model to temperature and precipitation changes. So far, the participation of CARAIB in the intercomparison exercises within MACSUR resulted in further improvements of the model by introducing new functionalities. [less ▲]

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See detailSoil Seed Bank : a poorly know component of forest regeneration
Douh, Chauvelin ULg; Daïnou, Kasso ULg; Fernandez Pierna, Juan-Antonio et al

Poster (2014, February)

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See detailPredicative Possession in Late Egyptian (with special attention to incipient grammaticalization processes)
Polis, Stéphane ULg

Conference (2014, February)

In this talk, I provide a description of the various types of constructions used for expressing clausal (‘predicative’) possession in Late Egyptian. The corpus is comprehensively defined to include both ... [more ▼]

In this talk, I provide a description of the various types of constructions used for expressing clausal (‘predicative’) possession in Late Egyptian. The corpus is comprehensively defined to include both literary and non-literary texts from the reign of Thutmose 3 (c. 1450 BC) down to abnormal hieratic texts (c. 600 BC), excluding most of the texts in Égyptien de tradition (i.e. purposely imitating various registers of EEg). The talk is structured in three sections that reflect both functional and structural features: (1) The adjectival predicate pattern or the marked expression of ownership; (2) The comitative strategy or the unmarked expression of possession; (3) Other types of predicative possession in Late Egyptian. [less ▲]

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See detailDamage Detection in Civil Engineering Structure Considering Temperature Effect
Nguyen, Viet Ha; Mahowald, Jean; Maas, Stefan et al

in Proceedings of IMAC XXXII Dynamics of Coupled Structures (2014, February)

This paper concerns damage identification of a bridge located in Luxembourg. Vibration responses were captured from measurable and adjustable harmonic swept sine excitation and hammer impact. Different ... [more ▼]

This paper concerns damage identification of a bridge located in Luxembourg. Vibration responses were captured from measurable and adjustable harmonic swept sine excitation and hammer impact. Different analysis methods were applied to the data measured from the structure showing interesting results. However, some difficulties arise, especially due to environmental influences (temperature and soil-behaviour variations) which overlay the structural changes caused by damage. These environmental effects are investigated in detail in this work. First, the modal parameters are identified from the response data. In the next step, they are statistically collected and processed through Principal Component Analysis (PCA) and Kernel PCA. Damage indexes are based on outlier analysis. [less ▲]

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Peer Reviewed
See detailBayesian methods for predicting and modelling winter wheat biomass
Mansouri, Majdi ULg; Dumont, Benjamin ULg; Destain, Marie-France ULg

Poster (2014, February)

The objectives of this paper are threefold. The first objective is to propose to use an Improved Particle Filtering (IPF) based on minimizing Kullback-Leibler divergence for crop models' predictions. The ... [more ▼]

The objectives of this paper are threefold. The first objective is to propose to use an Improved Particle Filtering (IPF) based on minimizing Kullback-Leibler divergence for crop models' predictions. The performances of the proposed technique are compared with those of the conventional Particle Filtering (PF) for improving nonlinear crop model predictions. The main novelty of this task is to develop a Bayesian algorithm for nonlinear and non-Gaussian state and parameter estimation with better proposal distribution. The second objective is to investigate the effects of practical challenges on the performances of state estimation algorithms PF and IPF. Such practical challenges include (i) the effect of measurement noise on the estimation performances and (ii) the number of states and parameters to be estimated. The third objective is to use the state estimation techniques PF and IPF for updating prediction of nonlinear crop model in order to predict winter wheat biomass. PF and IPF are applied at a dynamic crop model with the aim to predict a state variable, namely the winter wheat biomass, and to estimate several model parameters. Furthermore, the effect of measurement noise (e.g., different signal-to-noise ratios) on the performances of PF and IPF is investigated. The results of the comparative studies show that the IPF provides a significant improvement over the PF because, unlike the PF which depends on the choice of sampling distribution used to estimate the posterior distribution, the IPF yields an optimum choice of the sampling distribution, which also accounts for the observed data. [less ▲]

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See detailL'architecture religieuse rurale à la fin du Moyen Age et au début des Temps modernes : réflexions méthodologiques
Joly, Emmanuel ULg

Conference (2014, January 31)

The end of the medieval era and the beginning of modern times are marked by the increasing importance of urban centres. But this period of time is also crucial for the reconstruction of religious ... [more ▼]

The end of the medieval era and the beginning of modern times are marked by the increasing importance of urban centres. But this period of time is also crucial for the reconstruction of religious buildings in rural areas. As a few scholars like Luc-Francis Génicot have already pointed out, a large quantity of church works were going on during the 15th and 16th century in villages throughout Europe. Although architectural historians often ignore these « marginal » buildings, these are nonetheless products of human activity and must therefore find a place within the scientific study of architecture. This lecture will then present the way the researcher, using the most recent approaches in architectural history, can reintegrate these buildings into the discipline. To study these parish churches, the architectural historian is confronted with an important amount of items. He has to understand the reasons for such abundance of constructions as well as their distribution in time and space. While dealing with formal and stylistic questions, the researcher should avoid any normative attitude leading to consider these churches simply as places of stylistical conservatism. Finally, for these low documented buildings, the architectural historian should try to answer questions as to planning, financing and implementation of church works. As a consequence, he also has to identify the various interveners in the building process. [less ▲]

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