References of "Article"
     in
Bookmark and Share    
See detailLa Biologie clinique en pédiatrie: hypotonie et retard moteur
Misson, Jean-Paul ULg; Leroy, Patricia; Dubru, Jean-Marie ULg et al

in Acta Paediatrica Belgica (1996), 28(2), 95

Detailed reference viewed: 19 (2 ULg)
Peer Reviewed
See detailAcquisition of new words and phonological memory in Williams syndrome: A case study
Barisnikov, Koviljka; Van der Linden, Martial ULg; Poncelet, Martine ULg

in Neurocase : Case Studies in Neuropsychology, Neuropsychiatry & Behavioural Neurology (1996), 2

Detailed reference viewed: 25 (2 ULg)
Peer Reviewed
See detailCombined Liver - Spleen -small intestine grafting in a rat model: Role of transplanting additional lymphoid tissue on survival.
D'Silva, Milbhor; Pirenne, Jacques; Nakhleh, R. E. et al

in International Surgery (1996), (81), 109-114

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailCost effectiveness of treatment in osteoporosis
Reginster, Jean-Yves ULg

in Journal of Rheumatology (1996), 23(7), 1312-1313

Detailed reference viewed: 4 (1 ULg)
Full Text
Peer Reviewed
See detailExpression of protein encoded by varicella-zoster virus open reading frame 63 in latently infected human ganglionic neurons
Mahalingam, Ravy; Wellish, Mary; Cohrs, Randall et al

in Proceedings of the National Academy of Sciences of the United States of America (1996), 93(5), 2122-2124

The ganglionic cell type in which varicellazoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open reading frame 63 protein, VZV open reading ... [more ▼]

The ganglionic cell type in which varicellazoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open reading frame 63 protein, VZV open reading frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system. [less ▲]

Detailed reference viewed: 15 (3 ULg)
Peer Reviewed
See detailUn nouveau département de français à l'Université de Liège : ses précédents, ses missions, ses projets, son équipe
Defays, Jean-Marc ULg

in Français 2000 : Le point sur le Français langue étrangère (1996), (150-151), 60-62

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailDu gang au groupe social: une analyse socio-préventive
Comeron, Manuel ULg

in Cahiers de la Sécurité (1996)

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de ... [more ▼]

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de la recherche qui évalue aussi les politiques publiques de prévention. [less ▲]

Detailed reference viewed: 74 (10 ULg)
See detailLa nutrition et le couple "mère-enfant"
Hennart, P; Porignon, Denis ULg; Donnen, P

in Académie Royale des Sciences d'Outre-Mer (1996)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailEtude des groupements de macrophytes vasculaires aquatiques de la Semois en rapport avec la qualité globale des eaux et du milieu
Thoen, Daniel ULg; Roussel, Laurence; Nicolas, Jacques ULg

in Ecologie (1996), 27(4), 223-232

Les groupements de macrophytes aquatiques de la Semois (ca. 200 km de long) ont été décrits par l'analyse de 218 relevés de végétation réalisés durant l'année 1994. De l'amont vers l'aval, six groupements ... [more ▼]

Les groupements de macrophytes aquatiques de la Semois (ca. 200 km de long) ont été décrits par l'analyse de 218 relevés de végétation réalisés durant l'année 1994. De l'amont vers l'aval, six groupements végétaux ont été caractérisés. Deux zones principales peuvent être distinguées. Dans la zone amont, la végétation potentielle a été détruite ou remplacée par des groupements végétaux, plus résistants à la pollution organique et aux modifications physiques du milieu: groupement à Sparganium emersum et Potamogeton nanans; association à Potamogeton pectinatus. Dans la zone aval, la présence de groupements relevant du Ranunculetum fluitantis est plus conforme à la végétation potentielle et indique de meilleures conditions du milieu. Une analyse canonique des correspondances, sur les principales espèces aquatiques et les paramètres physico-chimiques de l'eau de 40 stations, a permis de regrouper des espèces présentant des affinités écologiques entre elles et de dégager leurs valeurs indicatrices relatives pour certains paramètres abiotiques. [less ▲]

Detailed reference viewed: 74 (17 ULg)
Peer Reviewed
See detailModulation of expression and assembly of vinculin during in vitro fibrillar collagen-induced angiogenesis and its reversal.
Deroanne, Christophe ULg; Colige, Alain ULg; Nusgens, Betty ULg et al

in Experimental Cell Research (1996), 224(2), 215-23

A model of collagen-induced in vitro angiogenesis was used to investigate the modulation of expression and assembly of focal adhesion plaque-associated proteins during the process of differentiation ... [more ▼]

A model of collagen-induced in vitro angiogenesis was used to investigate the modulation of expression and assembly of focal adhesion plaque-associated proteins during the process of differentiation. Human umbilical vein endothelial cells (HUVEC), first attached on an adhesive substratum (gelatin-, fibronectin-, or laminin-coated dish) or adherent collagen gel and then covered by an overlaying collagen get, organized within 3-4 days in tube-like structures (TLS). Removing the overlaying collagen gel from fully differentiated HUVEC induced a reversion of the process and HUVEC returned to a monolayer pattern. Modulations of focal adhesion-associated proteins occurring in HUVEC during the in vitro differentiation process and its reversal were investigated by Western blot analysis. A significant decrease of expression of vinculin, the integrin alpha2 subunit, talin, alpha-actinin, and actin was observed in TLS whereas the amount of FVIII-related antigen did not vary as compared to control monolayer cultures. During reversal, all the reduced proteins were markedly reexpressed. Human skin fibroblasts (HSF), submitted to the same experimental conditions, did not form TLS. Most of the focal adhesion proteins in HSF were similarly modulated by an overlaying collagen gel with the exception of vinculin, which was not modified. This particular protein was therefore more thoroughly investigated. In a nondifferentiated monolayer of HUVEC, a significant proportion of vinculin was organized into a detergent-resistant juxtamembranous structure (focal adhesion plaque) which disassembled early in TLS formation and reassembled during the reversal of the process. The reduction of vinculin during TLS formation was preceded by a downregulation of its mRNA while this mRNA was upregulated during reversal of the morphotype. These results suggest that the modulations of the cytoskeletal and focal adhesion proteins and more specifically of vinculin coupled to its subcellular redistribution are critical and early events in the cascade of mechanochemical signaling during in vitro angiogenesis induced by fibrillar collagen. [less ▲]

Detailed reference viewed: 20 (6 ULg)
Full Text
Peer Reviewed
See detailLa reconnaissance des répudiations. Note sous Cass., 11 décembre 1995
Carlier, Jean-Yves ULg

in Revue Trimestrielle de Droit Familial [=RTDF] (1996)

Detailed reference viewed: 11 (0 ULg)
Full Text
See detailRecherches sur le Paléolithique supérieur de la Moldavie
Otte, Marcel ULg; López Bayón, Ignacio; Noiret, Pierre ULg et al

in Anthropologica et Praehistorica : Bulletin de la Société Royale Belge d'Anthropologie et de Préhistoire (1996), 107

The Moldavian sequence is an illustration of all the phases of the Upper Palaeolithic. It starts with archaic industries (with bifacial foliate points), then is going on with local Aurignacian industries ... [more ▼]

The Moldavian sequence is an illustration of all the phases of the Upper Palaeolithic. It starts with archaic industries (with bifacial foliate points), then is going on with local Aurignacian industries, and finally show a very intensive development of Gravettian industries. Its geographical position gives it a key-position for the study of the relationships between the hills of Central Europe and the wide eastern plains. [less ▲]

Detailed reference viewed: 30 (0 ULg)
Full Text
Peer Reviewed
See detailPrediction of signal peptide functional properties: a study of the orientation and angle of insertion of yeast invertase mutants and human apolipoprotein B signal peptide variants.
Talmud, P.; Lins, Laurence ULg; Brasseur, Robert ULg

in Protein Engineering (1996), 9(4), 317-21

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using ... [more ▼]

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using molecular modelling of the invertase signal peptide we have analysed the hydrophobicity potential and the change in dielectric constant of the energy transfer, when the molecule moves from a hydrophobic to a hydrophilic phase at the simulated hydrophobic-hydrophilic interface. This modelling has been carried out on wild type and mutant invertase signal peptides of altered function, previously reported in the literature. While the predicted angle of insertion correlates with the measured extent of invertase secretion, with an optimum angle of 45 degrees, mutations that change the angle of orientation reduce the extent of invertase secretion. We have applied these same molecular modelling principles to the naturally occurring variants of the human apolipo-protein B (apoB) signal peptide, that confer a secretion defective phenotype when fused to yeast invertase and expressed in yeast. Our modelling thus identifies a strong correlation between the predicted angle of insertion of the signal peptide into the membrane and its ability to direct secretion. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailDe la patria potestas (puissance paternelle) à l'autorité parentale
Gerkens, Jean-François ULg

in L'enfant, avenir des droits de l'homme (1996)

Detailed reference viewed: 65 (0 ULg)
Peer Reviewed
See detailLes constructions analogiques du futur III
Winand, Jean ULg

in Revue d'Egyptologie (1996), 47

Detailed reference viewed: 26 (5 ULg)
Full Text
Peer Reviewed
See detailLocal orders in II-VI liquid compounds
Gaspard, Jean-Pierre ULg; Raty, Jean-Yves ULg; Ceolin, R. et al

in Journal of Non-Crystalline Solids (1996), 205-207

The structure of liquid CdTe, HgTe and ZnTe is studied by neutron scattering. Upon melting, drastically different behaviors are found: CdTe and ZnTe keep their fourfold coordination in the melt and remain ... [more ▼]

The structure of liquid CdTe, HgTe and ZnTe is studied by neutron scattering. Upon melting, drastically different behaviors are found: CdTe and ZnTe keep their fourfold coordination in the melt and remain semiconductors, whereas HgTe becomes sixfold coordinated and metallic. This dissimilarity is discussed in terms of the electronegativity difference and of the repulsive potential hardness. Entropic aspects are also discussed [less ▲]

Detailed reference viewed: 17 (0 ULg)
Peer Reviewed
See detailEffect Of Propylene Glycol Supplementation Around Parturition On Milk Yield, Reproduction Performance And Some Hormonal And Metabolic Characteristics In Dairy Cows
Formigoni, A.; Cornil, Mc.; Prandi, A. et al

in Journal of Dairy Research (1996), 63(1),

Detailed reference viewed: 45 (3 ULg)
Full Text
Peer Reviewed
See detailStructure-specific nuclease activity in yeast nucleotide excision repair protein RAD2
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1995), 270(50), 30194-8

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailDynamic properties of the transient network formed by telechelic ionomers studied by dynamic light scattering and dynamic mechanical analysis
Johannsson, Ragnar; Chassenieux, Christophe; Durand, Dominique et al

in Macromolecules (1995), 28(25), 8504-8510

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L ... [more ▼]

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L, which is well below the overlap concentration of the polymer chains (C*), a transient network is formed characterized by a plateau modulus at high frequencies and a relatively narrow viscoelastic relaxation time distribution. The intensity autocorrelation function of the transient network shows two relaxational modes: a relatively fast diffusional mode close to the co-operative diffusional mode of semidilute solutions of the unfunctionalized polymers and a slow broader mode which becomes independent of the scattering angle at a concentrations close to C*. The relation between this slow mode and the viscoelastic relaxation is discussed. Both the slow mode and the viscoelastic relaxation processes have an Arrhenius temperature dependence with the same activation energy, E(a) = 25 kJ/mol. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Peer Reviewed
See detailThe robustness of self-organized criticality against extinctions in a tree-like model of evolution
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Europhysics Letters [=EPL] (1995), 32(7), 613-618

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the ... [more ▼]

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the model leads to self-organized criticality. For r not equal 0, a transition from growing trees to finite ones takes place at some critical r(c) = 0.48 +/- 0.01 value. For 0 less than or equal to r less than or similar to r(c), self-organized criticality is thus robust against extinctions of the weakest species. The size distribution of avalanches follows a power law behaviour with an exponent 3/2 which seems to be independent of the parameter r. The growing trees are found to be self-similar with a non-universal fractal dimension D-f non-trivially ranging from 2 to 1 depending on the parameter r. This constraint model opens up the field of description of various possible physical events for such an evolution model. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailEIT: Extreme-Ultraviolet Imaging Telescope for the SOHO Mission
Delaboudinière, J.-P.; Artzner, G. E.; Brunaud, J. et al

in Solar Physics (1995), 162

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer ... [more ▼]

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer-coated optics will select spectral emission lines from Fe IX (171 Å), Fe XII (195 Å), Fe XV (284 Å), and He II (304 Å) to provide sensitive temperature diagnostics in the range from 6 × 10[SUP]4[/SUP] K to 3 × 10[SUP]6[/SUP] K. The telescope has a 45 x 45 arcmin field of view and 2.6 arcsec pixels which will provide approximately 5-arcsec spatial resolution. The EIT will probe the coronal plasma on a global scale, as well as the underlying cooler and turbulent atmosphere, providing the basis for comparative analyses with observations from both the ground and other SOHO instruments. This paper presents details of the EIT instrumentation, its performance and operating modes. [less ▲]

Detailed reference viewed: 53 (6 ULg)
Full Text
Peer Reviewed
See detailPre- and Post-Translational Regulation of Aromatase by Steroidal and Non-Steroidal Aromatase Inhibitors
Foidart, Agnès ULg; Tlemcani, O.; Harada, N. et al

in Brain Research (1995), 701(1-2), 267-78

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir ... [more ▼]

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir) cells and the concentration of aromatase mRNA as measured by RT-PCR in the brain. All these effects can be mimicked by estrogens. The behavioral effects of T can be blocked by a variety of aromatase inhibitors and, in parallel, the AA is strongly inhibited in the preoptic area (POA). We showed recently that the steroidal inhibitor, 4-OH-androstenedione (OHA) markedly decreases the immunostaining density of brain ARO-ir cells while the non-steroidal inhibitor, R76713 (racemic Vorozole; VOR) unexpectedly increased the density of this staining, despite the fact that the enzyme activity was completely inhibited. To generalize these findings and try to identify the underlying mechanism, we compared here the effects of two steroidal (OHA and androstatrienedione [ATD]) and two non-steroidal (VOR and Fadrozole [FAD]) aromatase inhibitors on the aromatase immunostaining and aromatase mRNA concentration in the brain of castrated quail concurrently treated with T. The 4 inhibitors significantly blocked the activation by T of male copulation. The two steroidal inhibitors decreased the immunostaining of brain ARO-ir cells but both VOR and FAD markedly enhanced the density of this staining. In parallel, OHA and ATD completely blocked the T-induced increase in aromatase mRNA concentration, while VOR and FAD had no effect on these RNA concentrations in the POA-anterior hypothalamus and they decreased them only slightly in the posterior hypothalamus. Taken together these results suggest that the inhibition of AA by ATD or OHA and the subsequent removal of locally produced estrogens blocks the synthesis of aromatase presumably at the transcriptional level. By contrast, the two non-steroidal inhibitors tested here block AA but in parallel increase the aromatase immunostaining. This effect does not result from an enhanced transcription and it is therefore speculated that these compounds increase either the translation of the aromatase mRNA or the half-life of the protein itself. [less ▲]

Detailed reference viewed: 20 (1 ULg)
See detailLes clivages entérinés dans la constitution belge
Verjans, Pierre ULg

in Agenda Interculturel (1995), (139), 19-24

Detailed reference viewed: 28 (1 ULg)
Peer Reviewed
See detailComment évaluer la sécrétion insulinique en pratique?
Scheen, A. J.; Paquot, Nicolas ULg; Letiexhe, M. R. et al

in Diabète & Métabolisme (1995), 21(6), 458-64

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailDiscussions about the Bp-d diagrams in polynomial for marine propeller series
Marchal, Jean ULg; Shen, Yide ULg; Kicheva, D.

in RINA (1995), volume 137

Detailed reference viewed: 39 (1 ULg)
See detailCécité et fantastique dans rapport sur les aveugles d'Alberto Breccia
Denis, Benoît ULg; Ghizzardi, Yves

in Voir (1995), (11), 52-63

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailThe Beta-Lactamase Secreted by the Antarctic Psychrophile Psychrobacter Immobilis A8
Feller, Georges ULg; Sonnet, P.; Gerday, Charles ULg

in Applied and Environmental Microbiology (1995), 61(12), 4474-6

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal ... [more ▼]

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailThe use of styrenic copolymers to generate polyimide nanofoams
Hedrick, James; Hawker, Craig J; DiPietro, R. et al

in Polymer (1995), 36(25), 4855-4866

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block ... [more ▼]

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block copolymers comprising a thermally stable block and a thermally labile material. At properly designed volume fractions, the morphology provides a matrix of the thermally stable material with the thermally labile material as the dispersed phase. Upon thermal treatment, the thermally unstable block undergoes thermolysis generating pores, the size and shape of which are dictated by the initial copolymer morphology. Several labile blocks were surveyed including polystyrene, poly(alpha-methylstyrene) and several alpha-methylstyrene/styrene copolymers. Each of these polymers can unzip to its monomer upon heating; however, the rate is substantially slower for polystyrene. The copolymers were synthesized through either the poly(amic acid) precursor, followed by chemical imidization to the polyimide form, or the poly(amic alkyl ester) precursor followed by thermal imidization. The decomposition of the labile coblock was studied by thermogravimetric and dynamic mechanical analysis. Upon decomposition, the foams showed pore sizes in the nanometre regime along with the expected reduction in mass density. [less ▲]

Detailed reference viewed: 35 (4 ULg)
Peer Reviewed
See detailIn vitro drug sensitivity and clinical aspects of Plasmodium falciparum malaria in African children
Carme, Bernard; Gay, Frederic; Ndounga, Mathieu et al

in Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (1995), 46(4), 270-274

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1 ... [more ▼]

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1: 39 asymptomatic school children, Group 2: 16 children with uncomplicated malaria, Group 3: 40 with severe but not pernicious malaria and Group 4: 20 with pernicious malaria. The drugs tested were chloroquine (CQ), quinine (QN) and mefloquine (MQ). The sensitivity level was assessed by a 48-hour in vitro maturation test involving the uptake of tritiated hypoxanthine, the initial blood level of parasite being > or = 0.1% in all cases. For QN and MQ, the median IC50 values showed no significant difference related to clinical status, age or parasitaemia levels. For CQ, the proportion of resistant strains and the 50 inhibitory concentration (IC50) values were greater in the cases of children hospitalised for malaria but there were no differences related to clinical severity of these hospitalised children nor, within each group, to the age or parasitaemia levels. The percentage of subjects with an IC50 value greater than the 90 percentile of the IC50 of the asymptomatic group, which we propose as the severity index related to chemoresistance, was 15% for uncomplicated malaria, 38% for severe but non-pernicious forms and 35% for pernicious malaria. The IC50 for QN was significantly higher in CQ-resistant strains and there was a positive correlation for CQ vs QN and for QN vs MQ. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Peer Reviewed
See detailPlasma Membrane-Dependent Activation of Gelatinase a in Human Vascular Endothelial Cells
Lewalle, J. M.; Munaut, Carine ULg; Pichot, B. et al

in Journal of Cellular Physiology (1995), 165(3), 475-83

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the ... [more ▼]

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the matrix metalloproteinase family, degrades BM type IV collagen and is involved in the BM breakdown by migrating tumor cells and endothelial cells (EC). Gelatinase A is synthesized as latent proenzyme and must be activated in order to express its proteolytic activity. A plasma membrane-dependent mechanism of activation has been described for several tumor and transformed cells lines. In the present study, we show that latent (72 kD) and mature (62-59 kD) forms of gelatinase A are present in EC membrane fraction from Triton X-114 extract while only latent form is found in the cytosolic fraction. The incubation of EC membrane fraction with exogenous latent gelatinase A resulted in a significant activation giving rise to 62-59 kD mature forms. 12-O-tetradecanoylphorbol-13-acetate (TPA), a strong potentiator of angiogenesis in vitro and in vivo, increases the amount of both latent and activated forms of gelatinase A in EC membrane fraction as well as the ability of this latter fraction to activate exogenous latent gelatinase A. We show that the mRNA transcript coding for the membrane-integrated MMP, the MT-MMP, previously described as a potential gelatinase A activator in invasive tumor cells is also expressed in vascular EC and is regulated through a TPA sensitive process. This enzyme may be responsible for membrane-dependent gelatinase A activation in normal vascular EC and may therefore be a determinant in the control of BM proteolysis during angiogenesis. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailTolerance to Osmotic Shocks in Rats Kidney Cortex and Medulla
Gilles, Raymond ULg; Compère, Philippe ULg; el Goumzili, M. et al

in Tissue & Cell (1995), 27(6), 667-77

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic ... [more ▼]

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic shocks induces dramatic modifications in chromatin organization. The present paper reports on the changes of medulla cell chromatin in situ, in rat kidney slices submitted to osmotic challenges and in vitro, on preparations of extracted chromatin submitted to changes in environmental ion concentrations. Our results show that the chromatin of kidney medulla cells: (1) does not behave differently from the other mammalian chromatins when submitted in situ or in vitro to osmotic challenges; (2) presents in vitro physico-chemical characteristics similar to those of the other mammalian chromatins; and (3) is protected in vitro, as the other mammalian chromatins, from the disrupting effects of increases in inorganic ion concentrations by different compensatory organic solutes. The ability of kidney medulla cells to adapt to large increases in osmolarity could thus be related to a rapid control of the level of such compounds rather than to some rather specific, intrinsic molecular adaptations of macromolecules. [less ▲]

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailBeef production in Belgium: to which extent manage extensification
Istasse, Louis ULg; Clinquart, Antoine ULg; Dufrasne, Isabelle ULg et al

in Proceeding of a workshop held in Paris on Novembre 22, 23 and 24, 1995 (1995)

Detailed reference viewed: 18 (2 ULg)
Peer Reviewed
See detailGenetic Mapping of Mitochondrial Markers by Recombinational Analysis in Chlamydomonas Reinhardtii
Remacle, Claire ULg; Colin, Martine ULg; Matagne, René-Fernand ULg

in Molecular & General Genetics [=MGG] (1995), 249(2), 185-90

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type ... [more ▼]

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type minus (paternal) parent. In contrast mitotic zygotes transmit maternal and paternal mitochondrial DNA copies to their diploid progeny and recombinational events between molecules of both origins frequently occur. Six mitochondrial mutants unable to grow in the dark (dk- mutants) were crossed in various combinations and the percentages of wild-type dk+ recombinants were determined in mitotic zygotes when all progeny cells had become homoplasmic for the mitochondrial genome. In crosses between strains mutated in the COB (apocytochrome b) gene and strains mutated in the COX1 (subunit 1 of cytochrome oxidase) gene, the frequency of recombination was 13.7% (+/- 3.2%). The corresponding physical distance between the mutation sites was 4.3 kb. In crosses between strains carrying mutations separated by about 20 bp, a recombinational frequency of 0.04% (+/- 0.02%) was found. Two other mutants not yet characterized at the molecular level were also used for recombinational studies. From these data, a linear genetic map of the mitochondrial genome could be drawn. This map is consistent with the positions of the mutation sites on the mitochondrial DNA molecule and thereby validates the method used to generate the map. The frequency of recombination per physical distance unit (3.2% +/- 0.7% per kilobase) is compared with those obtained for other organellar genomes in yeasts and Chlamydomonas. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailMutations Affecting the Mitochondrial Genes Encoding the Cytochrome Oxidase Subunit I and Apocytochrome B of Chlamydomonas Reinhardtii
Colin, Martine ULg; Dorthu, M. P.; Duby, Franceline ULg et al

in Molecular & General Genetics [=MGG] (1995), 249(2), 179-84

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase ... [more ▼]

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase pathway is still active the mutants have lost the capacity to grow heterotrophically (dark + acetate) and display reduced growth under mixotrophic conditions (light + acetate). In crosses between wild-type and mutant cells, the meiotic progeny only inherit the character transmitted by the mt- parent, which indicates that the mutations are located in the 15.8 kb linear mitochondrial genome. Two new mutants (dum-18 and dum-19) have now been isolated and characterized genetically, biochemically and at the molecular level. In addition, two previously isolated mutants (dum-11 and dum-15) were characterized in more detail. dum-11 contains two types of deleted mitochondrial DNA molecules: 15.1 kb monomers lacking the subterminal part of the genome, downstream of codon 147 of the apocytochrome b (COB) gene, and dimers resulting from head-to-head fusion of asymmetrically deleted monomers (15.1 and 9.5 kb DNA molecules, respectively). As in the wild type, the three other mutants contain only 15.8 kb mitochondrial DNA molecules. dum-15 is mutated at codon 140 of the COB gene, a serine (TCT) being changed into a tyrosine (TAC). dum-18 and dum-19 both inactivate cytochrome c oxidase, as a result of frameshift mutations (addition or deletion of 1 bp) at codons 145 and 152, respectively, of the COX1 gene encoding subunit I of cytochrome c oxidase. In a total of ten respiratory deficient mitochondrial mutants characterized thus far, only mutations located in COB or COX1 have been isolated.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 32 (4 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 20. Effect of monomer, solvent, and initiator on the ring-opening polymerization as initiated with aluminum alkoxides
Ropson, Nathalie; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(23), 7589-7598

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization ... [more ▼]

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization initiated by aluminum alkoxides. In toluene, aluminum triisopropoxide, a commonly used initiator, forms tetrameric (A(4)) and/or trimeric (A(3)) aggregates, which are dissociated upon the addition of the cyclic monomer. Actually, LA is responsible for the complete deaggregation of both A(3) and A(4) into single species (A(1)) trisolvated by the monomer (Al coordination number, CN = 6), whereas A(3) is selectively deaggregated by epsilon-CL. This behavior is consistent with the higher stability of A(4) compared to A(3). Whatever the monomer, three chains are initiated by the active A(1) species. When conversion of epsilon-CL is complete, the active aluminum trialkoxide sites are solvated by one (CN = 4) and more probably three (CN = 6) carbonyl groups of the attached poly-epsilon-caprolactone chains. At the complete LA conversion, only one carbonyl group of the polylactide chains is coordinated to Al (CN = 4). In THF, there is a competition between monomer and solvent for coordination to Al, which explains why polymerization is slower in THF than in toluene. In both toluene and THF, diethylaluminum monoalkoxide forms cyclic trimers that are solvated by the monomer (epsilon-CL) at least on the NMR time scale. Nevertheless, a fractional kinetic order in initiator is observed which gives credit to a fast reversible dissociation of the epsilon-CL-solvated trimers. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailPhase-shifting real time interferometry that uses bismut silicon oxide crystals
Georges, Marc ULg; Lemaire, Philippe

in Applied Optics (1995), 34(32), 7497-7506

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with ... [more ▼]

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with an interferogram-analysis method that uses the phase-shifting technique for quantitative measurement of diffusive-reflecting object deformations. In addition to the usual error sources in phase shifting, the temporal interferogram erasure is studied and is found weakly perturbative for the measured phase. Itis shown that quantitative measurements are possible for low-intensity object beams (8 μW/cm2) and a large observed area. Apractical situation of defect monitoring in a composite structure is presented. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailThe Replication in Vitro of the Gammaherpesvirus Bovine Herpesvirus 4 Is Restricted by Its DNA Synthesis Dependence on the S Phase of the Cell Cycle
Vanderplasschen, Alain ULg; Goltz, M.; Lyaku, J. et al

in Virology (1995), 213(2), 328-40

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to ... [more ▼]

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to determine the relationship between BHV-4 infection and the cell cycle. The temporal expression of BHV-4 late (L) proteins in unsynchronized cell cultures was first investigated by flow cytometry. Interestingly, L protein expression occurred in a limited number of cells infected with a high multiplicity of infection, and a reciprocal correlation between the percentage of positive cells and the cell density at the time of infection was demonstrated. Moreover, the finding that a BHV-4 early-late protein was expressed in nearly all the cells suggested that a blockage in the viral replication cycle occurred in some infected cells at the stage of viral DNA synthesis or L protein expression. Because this blockage could be the consequence of the dependence of one or both of these events on the cell cycle, they were investigated after infection of synchronized cell cultures. The following findings were made. (i) Cell transition through the S phase quantitatively increased the rate of BHV-4 DNA replication. (ii) BHV-4 DNA synthesis could not be detected in cells arrested in G0. (iii) Synchronization of MDBK cells with Lovastatin before infection increased the percentage of cells expressing L proteins. (iv) In contrast, infection of cells arrested in G0 led to few positive cells. Taken together these results showed that BHV-4 DNA replication and consequently the expression of L proteins are dependent on the S phase of the cell cycle. This dependence could be of importance for several biological properties of BHV-4 infection in vitro and might have implications for the biology of the virus in vivo. [less ▲]

Detailed reference viewed: 18 (4 ULg)
Peer Reviewed
See detailHighly-Expressed P100/P52 (Nfkb2) Sequesters Other Nf-Kappa B-Related Proteins in the Cytoplasm of Human Breast Cancer Cells
Dejardin, Emmanuel ULg; Bonizzi, Giuseppina; Bellahcene, Akeila ULg et al

in Oncogene (1995), 11(9), 1835-41

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the ... [more ▼]

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the molecular control of the transformed phenotype, we examined the expression of these proteins in human breast cancer cell lines as well as in primary tumors. Western Immunoblots demonstrated high expression of the p52 precursor p100 (NFKB2) in several breast cancer cell lines while human mammary epithelial cells express this protein only faintly. Eighteen primary breast tumors out of 24 displayed significant expression of the p100/p52 protein. In MDA-MB-435 cells, overexpressed p100 and p52 are predominantly cytoplasmic and coimmunoprecipitation experiments demonstrated that p100 sequesters the heterodimer p50/p65 in the cytoplasm. We demonstrate that most p65 protein is complexed with p100 in these cells while it is complexed predominantly with I kappa B-alpha in cell lines expressing less p100. Our data strengthen the hypothesis that NF-kappa B could be involved in carcinogenesis and suggest that the p100/p52 NF-kappa B subunit could play a role in the development of human breast cancers, possibly by sequestering other NF-kappa B-related proteins in the cytoplasm. [less ▲]

Detailed reference viewed: 54 (4 ULg)
Full Text
Peer Reviewed
See detailStellar calibration of CCD flat fielding.
Manfroid, Jean ULg

in Astronomy and Astrophysics. Supplement Series (1995), 113

The role of star observations is advocated in the photometric calibration of the low spatial-frequency non-uniformities of CCD detectors.

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailPhotometric monitoring (1987 to 1994) of the gravitational lens candidate UM 425.
Courbin, F.; Magain, Pierre ULg; Remy, M. et al

in Astronomy and Astrophysics (1995), 303

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged ... [more ▼]

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged through an R filter in order to obtain their light curves. The photometry was obtained by simultaneously fitting a stellar two-dimensional profile on each component. The brightest image (component A, m_R_=15.7) shows a slow and smooth increase in brightness of 0.2 magnitude in seven years, while the faintest one (component B, m_R_=20.1) displays an outburst of 0.4 magnitude which lasts approximately two years. The variation of component B may be interpreted in two ways, assuming UM 425 is gravitationally lensed. If it is due to an intrinsic variation of the quasar, we derive a lower limit of 3 years on the time delay from the fact that it is not observed in component A. On the other hand, if it is a microlensing "High Amplification Event", we estimate the size of the source to be ~10^-3^pc, in agreement with standard models of AGNs. These observations are consistent with the gravitational lens interpretation of the object. Furthermore, all the CCD frames obtained under the best seeing conditions have been co-added, in an attempt to detect the deflector. The final R image reveals a rich field of faint galaxies in the magnitude range m_R_~22-24. No obvious deflector, nor any system of arcs or arclets is detected, down to a limiting magnitude of m_R_~24. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detail"Music alone survives”? The collapse of faith in sonnets by Geoffrey Hill and G.M. Hopkins’
Pagnoulle, Christine ULg

in Cahiers Victoriens & Edouardiens (1995), 42

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's ... [more ▼]

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's most famous celebratory sonnet 'The Windhover.' [less ▲]

Detailed reference viewed: 46 (12 ULg)
Full Text
Peer Reviewed
See detailFactors influencing the immediate and late outcome of cushing-disease treated by transsphenoidal surgery : A retrospective study by the european cushings-disease survey group
Bochicchio, D.; Losa, M.; Buchfelder, M. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3114-3120

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of ... [more ▼]

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of patients in order to identify the prognostic factors influencing the outcome after transsphenoidal surgery. We conducted a multicenter, retrospective analysis of the early and late results of surgical treatment of Cushing's disease. Files of patients with Cushing's disease who underwent transsphenoidal surgery between 1975 and 1990 were collected from 25 institutions throughout Europe. Data from 668 of 716 patients were suitable for statistical analyses. Surgical mortality was 1.9%, and major morbidity occurred in 97 patients (14.5%). Clinical and biochemical remission of Cushing's disease after surgery occurred in 510 cases (76.3%). Identification of the tumor by neuroradiological imaging or at operation with histopathological corroboration was associated with remission of hypercortisolism. Recurrence of the disease occurred in 65 (12.7%) of 510 patients in remission after surgery at a mean time of 39.3 months (range 6-104 months). The distribution of the recurrences did not show any apparent plateau or cluster throughout the follow-up period. Low postoperative steroid levels, absence of cortisol response to CRH, and the need for long-term glucocorticoid substitution therapy were all associated with a high probability of long-term remission. Our study demonstrates that transsphenoidal surgery is a safe and effective treatment for patients with Cushing's disease. However, after successful surgery there is a steady increase in the percentage of recurrences, which continues with time. Patients who after operation had hypoadrenocorticism and needed long-term glucocorticoid substitution therapy had the lowest risk of relapse. [less ▲]

Detailed reference viewed: 228 (1 ULg)
Full Text
Peer Reviewed
See detailEffect of naloxone and metoclopropamide on LH secretion in a hyperprolactinemic, hypogonadotropic, postmenopausal woman, fertility and sterility.
Verhelst, J.; Beckers, Albert ULg; Abs, R.

in Fertility and Sterility (1995), 64(5), 969-971

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year ... [more ▼]

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year-old woman with a macroprolactinoma. INTERVENTIONS: Serial 10-hour IV infusions of naloxone and metoclopramide. MAIN OUTCOME MEASURE: Serum LH concentration. RESULTS: Naloxone induced a small but significant rise of serum LH levels, which displayed a pulsatile pattern. By contrast, metoclopramide elicited no significant response in LH secretion. CONCLUSION: Opioidergic but not dopaminergic neurotransmission plays a direct role in the suppression of LH secondary to hyperprolactinemia. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
Peer Reviewed
See detailPreparation of a Macroporous Biodegradable Polylactide Implant for Neuronal Transplantation
Schugens, C.; Grandfils, Christian ULg; Jérôme, Robert ULg et al

in Journal of Biomedical Materials Research (1995), 29(11), 1349-62

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an ... [more ▼]

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an original method consisting of the aggregation of calibrated poly-D,L-lactide microparticles. The size of the interstices between the aggregated microspheres was in a direct relationship to the microsphere diameter. A first approach was based on coating the microspheres with poly(vinyl alcohol) followed by chemically crosslinking the coating layers that were in mutual contact. This method was disregarded because of the acute cytotoxicity of glutaraldehyde used as the crosslinking agent, the absence of macroporosity, and the complete lack of flexibility. A physical technique of aggregation was then tested, which relied on the plasticization of poly-D,L-lactide microspheres with triethylcitrate to the point where microspheres strongly adhered to each other when they were in contact. This method has proved to be straightforward and definitely superior to the chemical approach, particularly with respect to cytotoxicity, control of macroporosity, and flexibility. A polymer support was thus successfully which was biodegradable, macroporous( interconnected pores of 10-100 microns in diameter), and flexible. This potential medical device is presently being used for neuronal transplantation in the central nervous system. [less ▲]

Detailed reference viewed: 29 (5 ULg)
Full Text
Peer Reviewed
See detailIs Sepsis a Mediator-Inhibitor Mismatch?
Lamy, Maurice ULg; Deby, Ginette ULg

in Intensive Care Medicine (1995), 21(Suppl 2), 250-7

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailEfficacy and Safety of Drugs for Paget's Disease of Bone
Reginster, Jean-Yves ULg; Lecart, M. P.

in BONE (1995), 17(5 Suppl), 485-488

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and ... [more ▼]

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and bisphosphonates are now considered as the main therapeutic approaches for this disease. Daily parenteral administration of calcitonin to patients with Paget's disease of bone results in a significant fall in serum alkaline phosphatase and urinary hydroxyproline levels. This treatment has also been reported to be effective in relieving clinical symptoms of the disease, mainly bone pain. The drawbacks of injectable calcitonin have stimulated interest in alternative routes of delivery. Substantial evidence of calcitonin bioavailability and bioefficacy equivalent to those of parenteral administration is currently available for only two alternative routes: nasal spray and rectal suppository. Since many results have been published showing a dramatic effect of several bisphosphonates in Paget's disease of bone, nasal and rectal calcitonin are no longer considered as the treatments of choice in this condition. A major advantage of the use of bisphosphonates over calcitonin in Paget's disease is that biochemical and histologic suppression of disease activity may persist for many years after the cessation of treatment. Oral etidronate and intravenous pamidronate have been extensively used and have provided satisfactory benefits to the patient. Since the risk/benefit ratio of alendronate does not appear to be completely positive, it is likely that the future of treatment of Paget's disease of bone will be based on the oral formulation of the new bisphosphonates, including tiludronate, risedronate or dimethyl-pamidronate. [less ▲]

Detailed reference viewed: 7 (2 ULg)
Peer Reviewed
See detailLe cas clinique du mois. Dépression majeure chez un patient diabétique
Pitchot, William ULg; Ansseau, Marc ULg

in Revue Médicale de Liège (1995), 50(11), 451-2

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailThe prevalence of colonic polyps in acromegaly : a prospective colonoscopic and pathological study in 103 patients.
Delhougne, B.; Deneux, C.; Abs, R. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3223-3226

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results ... [more ▼]

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results obtained in rather small series of patients or without a control group. We, therefore, undertook a prospective colonoscopic and pathological study comprising 103 acromegalic patients and 138 nonacromegalic control subjects referred for irritable bowel syndrome. The prevalence of adenomatous colonic polyps was significantly increased in acromegalic patients compared to that in control subjects (22.3% vs. 8.0%; P = 0.0024). The significance was similarly present in male acromegalic patients (28.6% vs. 5.5% in male control subjects; P = 0.0026), but was absent in female acromegalic patients. The prevalence of colonic polyps was also significantly increased in the group of acromegalic patients under 55 yr of age (20.0% vs. 3.0% in the control group of the same age; P = 0.0026). Other characteristics of adenomatous colonic polyps in acromegaly were the multiplicity and the presence proximal to the splenic flexure. No difference in the duration of acromegaly was found between patients with or without adenomatous polyps. The prevalence of hyperplastic colonic polyps was also significantly increased to 24.3% in acromegalic patients vs 4.4% in control subjects (P < 0.001). In conclusion, in view of the increased incidence of adenomatous colonic polyps, colonoscopy should be part of the follow-up examination in acromegaly. [less ▲]

Detailed reference viewed: 63 (6 ULg)
Peer Reviewed
See detailEvaluation and comparison of the behaviour at sea and forces in cables of two types of offshore tension leg platforms
Marchal, Jean ULg; Lejeune, A.

in International Symposium on CABLE DYNAMICS (1995)

Detailed reference viewed: 12 (5 ULg)
Full Text
Peer Reviewed
See detailRetinoic Acid Induces Three Newly Cloned Hoxa1 Transcripts in Mcf7 Breast Cancer Cells
Chariot, Alain ULg; Moreau, Louis; Senterre, Geoffrey et al

in Biochemical and Biophysical Research Communications (1995), 215(2), 713-20

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of ... [more ▼]

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of their cDNA sequences are still unknown. We report here the molecular characterization of three newly cloned HOXA1 transcripts from human breast cancer cells. In addition, we provide evidence that these alternatively spliced transcripts encode one homeodomain-containing protein and two products lacking the conserved DNA-binding domain. Moreover, we demonstrate that all three HOXA1 transcripts are induced by retinoic acid in MCF7 cells. Taken together, our results suggest that HOXA1 gene may be a key element in the establishment of the breast cancer cell phenotype. [less ▲]

Detailed reference viewed: 17 (6 ULg)
Full Text
Peer Reviewed
See detailAnionic polymerization of pivalolactone initiated by alkali metal alkoxides
Jedlinski, Zbigniew; Kurcok, Piotr; Kowalczuk, Marek et al

in Macromolecules (1995), 28(21), 7276-7280

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the ... [more ▼]

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the initiator, onto the carbonyl carbon atom of the monomer the selective cleavage of the acyl-oxygen bond of the monomer leads to the formation of alkoxide propagating species. Thus, it has been demonstrated that, in contrast to alpha-unsubstituted beta-lactones and higher lactones, the anionic polymerization of pivalolactone proceeds through either alkoxide or carboxylate propagation centers, depending on the nature of initiator used, i.e. an alkoxide or a carboxylate, respectively. In the former case, however, cyclic oligomers are also formed which are unusual in the anionic polymerization of other beta-lactones. This indicates that, whatever the lactone ring size (four-, six-, or seven-membered lactones), intramolecular transesterification reactions can take place if alcoholate ions are propagating species. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailLong-term photometry of variables at ESO. IV. The fourth data catalogue (1992-1994).
Sterken, C.; Manfroid, Jean ULg; Beele, D. et al

in Astronomy and Astrophysics. Supplement Series (1995), 113

In this paper we present the fourth catalogue of photometric data in the Stroemgren system obtained during the period June 18, 1992-August 23, 1994 in the framework of the Long-Term Photometry of ... [more ▼]

In this paper we present the fourth catalogue of photometric data in the Stroemgren system obtained during the period June 18, 1992-August 23, 1994 in the framework of the Long-Term Photometry of Variables (LTPV) program at the European Southern Observatory. The catalogue is available in computer readable form at the Centre de Donnees de Strasbourg via anonymous ftp 130.79.128.5. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailEffect of Plasma Anticonvulsant Level on Pipecuronium-Induced Neuromuscular Blockade: Preliminary Results
Hans, Pol ULg; Ledoux, Didier ULg; Bonhomme, Vincent ULg et al

in Journal of Neurosurgical Anesthesiology (1995), 7(4), 254-8

Patients receiving anticonvulsants are resistant to nondepolarizing muscle relaxants (NDMR). This study examines the effect of plasma anticonvulsant levels on pipecuronium-induced neuromuscular blockade ... [more ▼]

Patients receiving anticonvulsants are resistant to nondepolarizing muscle relaxants (NDMR). This study examines the effect of plasma anticonvulsant levels on pipecuronium-induced neuromuscular blockade. Twenty adult patients scheduled for neurosurgery were assigned to one of two groups. Group 0 (G0) consisted of 10 patients not on anticonvulsant therapy; group 1 (G1) included 10 patients treated either with phenytoin or carbamazepine. G1 patients were further divided into G1u (n = 4) and G1w (n = 6) subgroups, according to the plasma anticonvulsant level measured the day before surgery--under (G1u) or within (G1w) the therapeutic range. Neuromuscular transmission was monitored with a Biometer International A/S Accelograph. Anesthesia was induced and maintained using propofol and sufentanil. After calibration of the accelograph, a bolus of pipecuronium 0.08 mg/kg was given IV. The time from pipecuronium injection to the peak reduction of T1 was taken as the onset time. The time in min from pipecuronium injection to recovery of T1% (first accelograph response/baseline response) x 100 and TR% (fourth accelograph response/first accelograph response) x 100 were recorded at 25, 50, and 75% of baseline. The recovery index (RI) was taken as the time from 25 to 75% of baseline. The recovery index (RI) was taken as the time from 25 to 75% recovery of the baseline response. The onset time was not different in G0 (203 +/- 60.4 s), G1 (230.5 +/- 79.3 s), and G1u (181.8 +/- 60.4 s) but prolonged in G1w (279.2 +/- 67.7 s).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 23 (3 ULg)
Peer Reviewed
See detailLes cereales dans l'alimentation des bovins: valeur nutritionnelle et recommandations pour une utilisation sans risque.
Thewis, André ULg; Decruyenaere, Virginie

in Elevages Belges (1995), 49(10), 9-12

Detailed reference viewed: 149 (4 ULg)
Full Text
Peer Reviewed
See detailThe Impact of Renal Revascularisation on Renal Dysfunction
Van Damme, Hendrik ULg; Jeusette, F.; Pans, Alain ULg et al

in European Journal of Vascular and Endovascular Surgery : The Official Journal of the European Society for Vascular Surgery (1995), 10(3), 330-7

AIM: To determine the value of kidney revascularisation in patients with impaired renal function and correctable renal artery stenosis, the authors reviewed their surgical experience from 1978 to 1990 ... [more ▼]

AIM: To determine the value of kidney revascularisation in patients with impaired renal function and correctable renal artery stenosis, the authors reviewed their surgical experience from 1978 to 1990. PATIENTS AND METHODS: The study population included 23 patients with ischaemic nephropathy whose preoperative baseline creatinine level exceeded 20 mg/l (range 21-65 mg/l). This represents 20% of all patients operated on for renal artery disease during the same time interval. Preoperative risk profile, operative mortality, impact on hypertension and on renal function, and late survival were analysed. Renal function response to kidney revascularisation was defined as favourable (20% or more reduction of serum creatinine), moderate (stabilised serum creatinine values) or bad (further deterioration of renal function). All patients had atherosclerotic renal artery disease, involving a solitary kidney in five, both kidneys in 15 and one of the two kidneys in three patients. Hypertension was present in 74%. Revascularisation was unilateral in 10, bilateral in nine and associated with controlateral nephrectomy in four patients. RESULTS: Four patients died postoperatively (three myocardial infarctions, one stroke). Four patients needed postoperative short-term dialysis. After operation, renal function improved in 13, stabilised in six and deteriorated in four patients (of whom two died). Follow-up among the survivors averaged 46 months. The mean serum creatinine value at last follow-up visit was 26.2 mg/l, a decrease of 7.7 mg/l compared to preoperative values (p < 0.05). Overall, 69% of azotemic patients submitted to renal revascularisation manifested a favourable response (45% improved and 24% stabilised). Three patients required long-term dialysis. The 5-year survival rate was 48%. CONCLUSION: These data suggest that kidney revascularisation in patients with ischaemic nephropathy can restore or stabilise renal function, preventing evolution and end-stage renal disease and dialysis dependency. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailLack of universality in 2-dimensional multicomponent spreading phenomena
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1995), 52(4), 3447-3454

Magnetic entities are introduced in a two-dimensional Eden model as additional degrees of freedom in order to model multidomain spreading phenomena. The elements of the growth are "spins" taking q states ... [more ▼]

Magnetic entities are introduced in a two-dimensional Eden model as additional degrees of freedom in order to model multidomain spreading phenomena. The elements of the growth are "spins" taking q states and are coupled or not via an energy J, as in the Potts model, thus leading to a competition between species. The internal cluster spreading is found to result from a competition between growing domains. Complex mechanisms such as trapping, jamming and coalescence occur between the growing domains. A large variety of critical and nanuniversal regimes, from subcritical to self-organized critical behaviors, are obtained depending on nonuniversal parameters such as the lattice structure, the number of internal degrees of freedom q, and the coupling J. For the square lattice, the fractal dimension is 1.50 and the mass distribution exponent tau is 1.63. For the triangular lattice, the fractal dimension varies from 1.70 to 1.83 depending on the coupling value and the mass distribution exponent tau also varies from 1.67 to 1.98 depending on the coupling value. The correspondence and differences with respect to the percolation phenomenon are outlined. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailLumière and his View : the Cameraman's Eye in Early Cinema
Belloi, Livio ULg

in Historical Journal of Film, Radio & Television (1995), 15

Analyse des scènes de rue produites par la firme Lumière comme résultats d'une interaction entre l'opérateur et les sujets filmés.

Detailed reference viewed: 69 (8 ULg)
Full Text
Peer Reviewed
See detailAntioxidant Defense and Free Radical Production in a Rabbit Model of Kidney Ischemia-Reperfusion
Franssen, Colette ULg; Defraigne, Jean-Olivier ULg; Detry, Olivier ULg et al

in Transplantation Proceedings (1995), 27(5), 2880-3

Detailed reference viewed: 42 (0 ULg)
Peer Reviewed
See detailExercise in the Management of Non-Insulin-Dependent (Type 2) Diabetes Mellitus
Gautier, J. F.; Scheen, André ULg; Lefebvre, P. J.

in International Journal of Obesity & Related Metabolic Disorders (1995), 19(Suppl 4), 58-61

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailAdsorption behavior of polymers with ionic end groups : mono- and difunctional polystyrenes in toluene
Pankewitsch, T.; Vanhoorne, Pierre ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(20), 6986-6992

The adsorption behavior of mono- and difunctional telechelic polymers, omega- and alpha,omega-sulfonato polystyrenes, from dilute toluene solution on the surface of a silicon wafer is investigated by ... [more ▼]

The adsorption behavior of mono- and difunctional telechelic polymers, omega- and alpha,omega-sulfonato polystyrenes, from dilute toluene solution on the surface of a silicon wafer is investigated by ellipsometry. Polystyrene without ionic end groups does not adsorb, and the attachment of molecules to the silicon dioxide surface proceeds via the functional ionic ends. Already a single ionic group promotes adsorption, and adsorption kinetics at small times is found to be diffusion limited. The approach of an equilibrium adsorbed amount at later times is controlled by the hindered diffusion of chains through the already existing layer. With monofunctional chains a thin stable monolayer at the surface is formed. With difunctional chains there is strong indication for coupling of ionic ends and the formation of additional layers. It is caused by the interaction of dangling ends, when only one end of the chains is adsorbed at the surface. The molecular weight dependence of the grafting density is consistent with scaling predictions, and differences in the adsorbed amount of telechelics with different counterions are explained on the basis of different valencies. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailSmall-angle neutron scattering studies of low-polarity telechelic ionomer solutions. 1. Total scattering
Karayianni, Eleni; Jérôme, Robert ULg; Cooper, Stuart L

in Macromolecules (1995), 28(19), 6494-6507

The overall structure and thermodynamic interactions that take place,in low-polarity ionomer solutions have been investigated via the technique of small-angle neutron scattering for two Na-neutralized ... [more ▼]

The overall structure and thermodynamic interactions that take place,in low-polarity ionomer solutions have been investigated via the technique of small-angle neutron scattering for two Na-neutralized carboxy-telechelic polystyrene model ionomer systems that differ in the length of the polymer backbone. The strong interchain association that is evidenced in the viscometric measurements of the ionomers compared to the ester forms is apparent in the scattering profiles of the ionomers that show a characteristic upturn that extends in the low-q regime and increases with increasing polymer concentration. The apparent molecular weight and size of the aggregated particles show a significant increase over a small concentration range, while the ionic content seems to not affect the degree of association in the concentration regime studied. The supramolecular structure of the ionomer solutions is revealed by fitting the scattering data to a scaling law in the intermediate qR(g) regime that supports a percolation type of behavior for the ionomer clusters for both systems studied. The fractal dimension of the aggregates is 1.76 for both ionomer systems which provides evidence for a rather extended configuration and high polydispersity of the clusters in solution. The molecular weight of the associating particles is observed to rapidly approach infinity with increasing polymer concentration, indicating the approach to a gel transition. The scattering data of the ionomer solutions were fitted to the de Gennes model based on the random-phase approximation theory. The Flory-Huggins interaction parameter of the ionomer solutions determined using this model shows a strong concentration dependence with a quite unexpected increase with decreasing polymer concentration. The thermodynamic interactions in the ionomer solutions were changed dramatically by the association as revealed by a significant decrease of the second virial coefficient. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailOn the electrochemical polymerization of acrylonitrile and N-vinylpyrrolidone : new insight into the mechanism
Jérôme, Robert ULg; Mertens, Marc; Martinot, Lucien

in Advanced Materials (1995), 7(9), 807-809

The protection of metals against environmental attack can be achieved by coating the metal with a protective organic film. Poor adhesion of the organic film to the metal surface has, however, limited the ... [more ▼]

The protection of metals against environmental attack can be achieved by coating the metal with a protective organic film. Poor adhesion of the organic film to the metal surface has, however, limited the success of this approach. Here it is demonstrated that polyacrylonitride can be elecrografted onto a nickel surface producing a strongly adherent film. The transformation of the organic film could also open the way to new polymer-metal composites. [less ▲]

Detailed reference viewed: 39 (2 ULg)
Peer Reviewed
See detailThe kinetic growth anisotropy of the 123-(RE)BaCuO compounds
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Philosophical Magazine A (1995), 72(3), 727-736

The kinetic growth anisotropy of the melt-textured 123-(RE)BaCuO compounds, especially along the CuCO2 planes, is discussed here in detail. A computer model which simulates the grain growth of 123 ... [more ▼]

The kinetic growth anisotropy of the melt-textured 123-(RE)BaCuO compounds, especially along the CuCO2 planes, is discussed here in detail. A computer model which simulates the grain growth of 123 materials in the ab plane is presented. This kinetic growth model takes some bond anisotropy along the [100] and [110] directions into account. The model presented herein belongs to a hierarchical set of generalized Eden models as discussed in the Appendix. For different bond anisotropy parameters, the simulated 123-(RE)BaCUO grains exhibit square and octogonal overall shape which have been both optically observed. From simple kinetic arguments, we show that the 'faceting' of the 123-(RE)BaCuO grains finds its origin in a bond anisotropy rather than in a growth-rate anisotropy along some crystallographic directions. This is of general interest and opens new perspectives for developing the Eden model in the crystal-growth field. [less ▲]

Detailed reference viewed: 30 (0 ULg)
Full Text
Peer Reviewed
See detailSystemic Immune Response after Rectocolonic Administration of Ovalbumin in Mice
Louis, Edouard ULg; Franchimont, D.; Lamproye, Anne ULg et al

in International Archives of Allergy & Immunology (1995), 108(1), 19-23

The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c ... [more ▼]

The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c mice. One rectocolonic, but not intragastric, administration of 25 mg of ovalbumin induced a detectable increase in serum anti-ovalbumin antibody level. The level reached was however much lower than after subcutaneous injection. Both intragastric and rectocolonic immunization with ovalbumin induced specific systemic cellular tolerance. However, after rectocolonic, but not intragastric, preimmunization there was no systemic humoral tolerance to this antigen. These differences in systemic immune responses after rectocolonic or intragastric administration of ovalbumin could be due to different stimulation of the systemic immune system or to differences between the colonic and small bowel mucosal immune system, which remain to be elucidated. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Peer Reviewed
See detailLe cas clinique du mois. Rémission spontanée prolongée d'un diabète de type 1 typique
Scheen, A. J.; Letiexhe, M. R.; Paquot, Nicolas ULg et al

in Revue Médicale de Liège (1995), 50(9), 363-4

Detailed reference viewed: 34 (0 ULg)
Full Text
Peer Reviewed
See detailHypertension artérielle et résistances vasculaires
Krzesinski, Jean-Marie ULg; Rorive, Georges ULg

in Revue Médicale de Liège (1995), 50(9), 365-372

L'anomalie hémodynamique caractéristique de l'hypertension artérielle est l'augmentation des résistances vasculaires. Ces résistances siègent principalement au niveau de petites artères et d'artérioles ... [more ▼]

L'anomalie hémodynamique caractéristique de l'hypertension artérielle est l'augmentation des résistances vasculaires. Ces résistances siègent principalement au niveau de petites artères et d'artérioles précapillaires de diamètre inférieur à 300Il et sont le résultat de modifications essentiellement anatomiques. La média est de loin la couche la plus épaisse de la paroi de ces vaisseaux caractérisés,au stade d'hypertension chronique, par un rapport épaisseur de média sur lumière accru. Contrairement à l'animal hypertendu, cette modification est, chez l'homme, liée à un remodelage vasculaire (réarrangement cellulaire sans vraie hypertrophie ou hyperplasie), en relation avec une anomalie endothéliale (réduction de production de monoxyde d'azote, augmentation de production d'endothéline-l). L'intérêt pour ces modifications structurelles réside dans l'espoir d'influencer favorablement ces lésions notamment par des traitements spécifiques. Récemment, une étude canadienne a rapporté qu'un inhibiteur de l'enzyme de conversion, à même efficacité antihypertensive qu'un bêtabloquant, était seul capable de normaliser les anomalies structurelles de la média et de corriger (partiellement) la dysfonction endothéliale caractérisant également l'hypertendu après 27ms de traitement (étude chez l'homme, à partir de biopsies répétées de tissu sous-cutané, selon la technique proposée par les groupes de Heagerty et Mulvany). Cette observation jointe aux données expérimentales et à quelques données humaines suggère que les inhibiteurs de l'enzyme de conversion posséderaient bien des propriétés indépendantes de leur effet antihypertenseur permettant une correction des anomalies vasculaires dans l'hypertension. Ces travaux méritent cependant une confirmation sur un grand nombre de patients. Ils ont l'intérêt d'ajouter une note explicative pathogénique aux bénéfices déjà observés en épidémiologie dans la décompensation cardiaque, l'infarctus myocardique ou la protection rénale lors de l'utilisation de ces molécules. [less ▲]

Detailed reference viewed: 44 (0 ULg)
Full Text
See detailEspace et mémoire
Frankignoulle, Pierre ULg

in Le Magazine Liège Université (1995)

Detailed reference viewed: 19 (3 ULg)
Peer Reviewed
See detailIncidence and direct social costs of hip fractures from 1984 to 1993 in Belgium
Gillet, Pierre ULg; Reginster, Jean-Yves ULg

in Journal of Bone and Mineral Research (1995)

Detailed reference viewed: 11 (4 ULg)
Full Text
Peer Reviewed
See detailCytogenetic study of bovine oocytes matured in vitro
Ectors, Fabien ULg; Koulischer, Lucien ULg; Jamar, M. et al

in Theriogenology (1995), 44(3), 445-450

Described in the present paper is a cytogenetic study of bovine oocytes matured in vitro. The cumulus-oocyte complexes (COC), punctured from ovaries recovered in a local slaughterhouse, were classified ... [more ▼]

Described in the present paper is a cytogenetic study of bovine oocytes matured in vitro. The cumulus-oocyte complexes (COC), punctured from ovaries recovered in a local slaughterhouse, were classified into 3 groups according to follicular diameter 1 to 4mm, 5 to 8mm and 9 to 13 mm. Metaphases available for observation were classified as metaphase I, haploid and diploid metaphase II. High levels of haploid metaphases II (90.6, 86.9 and 94.4 %) among the 3 groups of follicular sizes indicated successful meiotic resumption during in vitro maturation and suggested that cytoplasmic maturation may be responsible for low developmental rate after IVM, IVF and in vitro development (IVD). [less ▲]

Detailed reference viewed: 27 (3 ULg)
Full Text
Peer Reviewed
See detailPurification of Progelatinases A and B by Continuous-Elution Electrophoresis
Remacle, A. G.; Baramova, E. N.; Weidle, U. H. et al

in Protein Expression & Purification (1995), 6(4), 417-22

Progelatinases A and B were purified from HT1080-conditioned culture medium using a continuous-elution electrophoresis. Initially cell culture medium was ammonium sulfate precipitated. The concentrated ... [more ▼]

Progelatinases A and B were purified from HT1080-conditioned culture medium using a continuous-elution electrophoresis. Initially cell culture medium was ammonium sulfate precipitated. The concentrated proteins were affinity chromatographed on gelatin-Sepharose column. The bound gelatinases were eluted with electrophoresis sample buffer and subjected to continuous-elution electrophoresis. In a single run, under the standardized working conditions, the obtained fractions contained four purified enzymes--progelatinase A (M(r) 72,000), its activated forms (M(r) 62,000 and M(r) 59,000), and progelatinase B (M(r) 92,000). Moreover, the continuous-elution electrophoresis allowed the enzymes separation from their respective inhibitors--TIMP-1 (M(r) 28,500) and TIMP-2 (M(r) 21,000). The purified progelatinases A and B demonstrated high specific activities (150-200 U/micrograms). [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
See detailPharma-clinics. Le médicament du mois. La terbinafine (Lamisil)
Pierard, Gérald ULg

in Revue Médicale de Liège (1995), 50(8), 352-3

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailMicroencapsulation by coacervation of poly(lactide-co-glycolide). IV. Effect of the processing parameters on coacervation and encapsulation
Nihant, N.; Grandfils, Christian ULg; Jérôme, Robert ULg et al

in Journal of Controlled Release (1995), 35(2-3), 117-125

Attention has been paid to phase separation of poly (lactide-co-glycolide) solutions in CH2Cl2 induced by the addition of a silicone oil in order to promote protein microencapsulation. Since the process ... [more ▼]

Attention has been paid to phase separation of poly (lactide-co-glycolide) solutions in CH2Cl2 induced by the addition of a silicone oil in order to promote protein microencapsulation. Since the process is very fast, the system is anytime out of equilibrium. The effect of the main processing parameters on the microencapsulation process has been analyzed and has highlighted that kinetics of the main encapsulation steps has a great effect on the characteristics of the final microspheres. These results have been discussed on the basis of a physico-chemical study of coacervation reported in previous papers of this series. [less ▲]

Detailed reference viewed: 95 (4 ULg)
Full Text
Peer Reviewed
See detailGlycoproteins of the aspartyl proteinase gene family secreted by the developing placenta
Roberts, R. M.; Xie, S.; Nagel, R. J. et al

in Advances in Experimental Medicine and Biology (1995)

Pregnancy in cattle and sheep can be diagnosed by the presence of placentally-derived antigens (pregnancy-associated glycoproteins or PAG-1) in maternal serum soon after implantation begins at about Day ... [more ▼]

Pregnancy in cattle and sheep can be diagnosed by the presence of placentally-derived antigens (pregnancy-associated glycoproteins or PAG-1) in maternal serum soon after implantation begins at about Day 20 following conception. Molecular cloning of their cDNA has revealed that PAG-1 belong to the aspartic proteinase gene family and have about 50% amino acid sequence identity to pepsin. However, critical amino acid substitutions at the active site regions suggest that both bovine and ovine PAG-1 are enzymatically inactive. PAG-1 expression has been shown by in situ hybridization and immunocytochemistry to be localized to the trophoblast binucleate cells, which invade maternal uterine endometrium during implantation. The glycoproteins are concentrated in dense cytoplasmic granules that are discharged after the binucleate cells have migrated to the maternal side of the placental barrier. We suggest, therefore, that the PAG-1 might have an endocrine function either as carriers of other bioactive peptides or by acting as hormones themselves. Recently screening of placental libraries with nucleic acid probes has identified additional cDNA that are very abundant and code for polypeptides (PAG-2 and PAG-3) related to, but antigenically and structurally distinct from PAG-1 described above. These molecules have sequences of amino acids at their catalytic centers that are consistent with their being potentially functional proteinases but their role during pregnancy, like that of PAG-1, is unclear. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailApproaches to the optimal navigation organization on the inland waterway network
Marchal, Jean ULg; Zhang, Z. M.

in Procceedings of the First Symposium on Operation Research with Application in Engineering, Technology and Management (ISORA’95) (1995)

Detailed reference viewed: 13 (3 ULg)
Full Text
Peer Reviewed
See detailDetection of Nucleic Acids in the Attomole Range Using Polybiotinylated Oligonucleotide Probes
Zecchini, Vincent; Caucheteux, Danièle; Gretry, Jeau et al

in BioTechniques (1995), 19(2), 286-90

This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were ... [more ▼]

This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were determined. The quality of avidin-peroxidase conjugate and the choice of chromogenic substrate influenced detection sensitivity. A signal amplification method was also developed for avidin enzymatic conjugates. These improvements allowed the detection of less than 0.02 fmol of target DNA. [less ▲]

Detailed reference viewed: 8 (2 ULg)
Peer Reviewed
See detailSelf-organized criticality in phylogenetic-like tree growths
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Journal de Physique I (1995), 5(8), 1011-1025

A simple stochastic model of Darwinistic evolution generating phylogenetic-like trees is developed. The model is based on a branching process taking competition-correlation effects into account. In ... [more ▼]

A simple stochastic model of Darwinistic evolution generating phylogenetic-like trees is developed. The model is based on a branching process taking competition-correlation effects into account. In presence of finite and short range correlations, the process self-organizes into a critical steady-state in which intermittent bursts of activity of all sizes are generated. On a geological-like time scale, this behaviour agrees with punctuated equilibrium features of biological evolution. The simulated phylogenetic-like trees are found to be self-similar. The dynamics of the transient regimes show a power law decrease of the order parameter towards the 0(+) value which characterizes an unstable critical state. The genetic range k of competition-correlations between living species is found to be a relevant parameter which determines the universality class of the evolution process. An infinite competition-correlation range destroys however the self-organized critical behaviour. The fractal dimension D-f of the phylogenetic-like trees increases from 2.0 to infinity as k goes from 1 to infinity. The critical exponent tau of avalanche size-distribution decreases from about 3/2 (for k = 1) and reaches about 1.2 for k = 10. A hyperscaling relation seems to relate the various universality classes. Through a mean-field theory, we mention that the evolution process is much more complex than a simple uncorrelated branching process. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailSoluble Interleukin-2 Receptor in Crohn's Disease. Assessment of Disease Activity and Prediction of Relapse
Louis, Edouard ULg; Belaiche, Jacques ULg; Van Kemseke, Catherine ULg et al

in Digestive Diseases & Sciences (1995), 40(8), 1750-6

In Crohn's disease, the activity of the disease is difficult to evaluate and the evolution of the disease is difficult to predict. The soluble interleukin-2 receptor serum level has been reported to ... [more ▼]

In Crohn's disease, the activity of the disease is difficult to evaluate and the evolution of the disease is difficult to predict. The soluble interleukin-2 receptor serum level has been reported to correlate with clinical activity of the disease and with mucosal immune activation. We compared serum soluble interleukin-2 receptor to classical inflammatory markers and other immune parameters in the assessment of clinical disease activity and prediction of relapse in patients with Crohn's disease. Soluble interleukin-2 receptor serum levels correlated well with the Crohn's disease activity index, and multivariate analysis showed that this correlation was independent of the other inflammatory and immune markers. The correlation was not greater, However, than that between some inflammatory markers, such as ESR, and Crohn's disease activity index. Longitudinal follow-up showed that a high soluble interleukin-2 receptor serum level was highly predictive of relapse. Multivariate analysis showed that the soluble interleukin-2 recepteur serum level was complementary to other inflammatory and clinical markers in the prediction of relapse of disease. We conclude that soluble interleukin-2 receptor is of use in monitoring Crohn's disease, particularly in prediction of relapse. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailAggregation behavior of ω- and α,ω-metal sulfonato polystyrene in toluene
Vanhoorne, Pierre ULg; Jérôme, Robert ULg

in Macromolecules (1995), 28(16), 5664-5670

The aggregation behavior of omega- and alpha,omega-metal sulfonato polystyrene in toluene has been investigated by static and dynamic light scattering. Monofunctional chains reversibly associate into ... [more ▼]

The aggregation behavior of omega- and alpha,omega-metal sulfonato polystyrene in toluene has been investigated by static and dynamic light scattering. Monofunctional chains reversibly associate into small reverse micelles. Lithium-based ionic aggregates contain an average of 12 ion pairs, which corresponds to a preferential structure for the ionic cores. When the molecular weight of the chains is increased, polymer-solvent interactions progressively distort the ionic aggregates, and above a critical molecular weight, estimated to be 50000 in toluene, the multiplets collapse into smaller aggregates of an average of 3 ion pairs. Traces of polar solvents do not strongly affect the aggregation number, but do weaken the dipole-dipole interactions. Structure of the ionic cores strongly depends on the nature of the ion pairs, which suggests that this structural feature is a key parameter in the control of the structure-property relationships in ionomers. The aggregation behavior of the difunctional chains is complex, due to the occurrence of a closed association mechanism for the individual multiplets and an open association of reverse micelles into larger aggregates. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailAPRIL 1993 ARCTIC PROFILES OF STRATOSPHERIC HCL, CLONO2, AND CCL2F2 FROM ATMOSPHERIC TRACE MOLECULE SPECTROSCOPY ATLAS 2 INFRARED SOLAR OCCULTATION SPECTRA
Rinsland, C. P.; Gunson, M. R.; Abrams, M. C. et al

in Journal of Geophysical Research (1995), 100(D7), 14019-14027

Partitioning among the major components of the stratospheric odd chlorine family inside and outside of the remanent Arctic vortex has been studied on the basis of infrared solar occultation measurements ... [more ▼]

Partitioning among the major components of the stratospheric odd chlorine family inside and outside of the remanent Arctic vortex has been studied on the basis of infrared solar occultation measurements obtained by the atmospheric trace molecule spectroscopy (ATMOS) Fourier transform spectrometer during the ATLAS 2 shuttle mission (April 8-17, 1993). Profiles of hydrogen chloride (HCl) and simultaneous profiles of chlorine nitrate (ClONO2) and CFC-12 (CCl2F2) are reported for examples of in-vortex and out-of-vortex conditions. Increased ClONO2 volume-mixing ratios (VMRs) are measured in the vortex below 20 mbar (similar to 25 km altitude) with a peak ClONO2 VMR of 2.05 +/- 0.45 ppbv (10(-9) per volume) at 56 mbar (similar to 19 km altitude). The reported error correspond to la uncertainties. Simultaneous CCl2F2 and N2O measurements, combined with published empirical relations, indicate that only 0.34 +/- 0.15 ppbv, about 10% of total chlorine, was bound in organic species at the ClONO2 VMR peak in the vortex. A colocated vortex profile of HCl, referenced to simultaneous N2O VMR measurements, has been used to derive a HCl mixing ratio of 1.21 +/- 0.12 ppbv corresponding to the ClONO2 VMR peak. The internal consistency of the ATMOS measurements is demonstrated by the agreement between the total chlorine mixing ratio of 3.60 +/- 0.72 ppbv derived at the ClONO2 VMR peak in the vortex and HCl measurements of 3.37 +/- 0.37 and 3.76 +/- 0.41 ppbv at 0.56 mbar, where HCl is the only significant chlorine-bearing molecule. Outside the vortex the mixing ratio of HCl exceeds the mixing ratio of ClONO2 throughout the stratosphere. [less ▲]

Detailed reference viewed: 13 (2 ULg)
Full Text
Peer Reviewed
See detailBlends of biphasic ionomers with chemically identical mono- and bifunctional oligomers
Plante, Michel; Bazuin, C Géraldine; Jérôme, Robert ULg

in Macromolecules (1995), 28(15), 5240-5247

Biphasic Cs and Ba carboxylated polystyrene ionomers were blended with chemically identical mono- and bifunctional oligomers whose chain lengths are significantly greater than the average segmental length ... [more ▼]

Biphasic Cs and Ba carboxylated polystyrene ionomers were blended with chemically identical mono- and bifunctional oligomers whose chain lengths are significantly greater than the average segmental length between ionic groups in the ionomer. The ionomers were chosen to have two ion contents such that, in one case, the matrix phase is dominant and, in the other, the cluster phase is dominant. From dynamic mechanical thermal analysis, it was observed that the biphasic morphology of the ionomers is preserved in all blends (containing up to 60 wt % oligomer). The monofunctional oligomers tend to have a mild plasticizing action on the two phases. In the matrix-dominated ionomer, the bifunctional oligomer has an antiplasticization effect at lower oligomer contents; this is attributed to a greater dispersion of ionic aggregates in the matrix phase, accompanied by pinning of the oligomer extremities in multiplets, which prevents plasticizing action. In the cluster-dominated ionomer, the oligomer forms a separate phase, which is related to insufficiently large ion-poor styrene regions in the ionomer to accommodate the long nonionic portion of the oligomer chains. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailStereocomplexation and morphology of polylactides
Brochu, Sylvie; Prud'homme, Robert E; Barakat, Ibrahim et al

in Macromolecules (1995), 28(15), 5230-5239

Blends of isotactic polylactides of opposite configurations lead to the formation of stereocomplexes, provided the enantiomeric excess of the two homopolymers in contact is high enough. In this study, the ... [more ▼]

Blends of isotactic polylactides of opposite configurations lead to the formation of stereocomplexes, provided the enantiomeric excess of the two homopolymers in contact is high enough. In this study, the stereocomplex formation between a poly(L-lactide) (100L) having an enantiomeric excess of 100% and a poly(D-lactide) having an enantiomeric excess of 80% (80D) has been investigated using differential scanning calorimetry and optical microscopy. These results have been compared to those obtained between two polylactides having both an enantiomeric excess of 100% (100L/100D blend). The melting temperatures of blends 100L/100D and 100L/80D are 230 and 208 degrees C, respectively. In both cases, the stereocomplex formation is preferred to the homopolymer crystallization and the stereocomplex controls the morphology of the blends over a wide range of concentrations. However, this control is more rigorous when the D component is 100% isotactic. This difference leads to a more complete crystallization of homopolymer 100L and to a greater influence of crystallization conditions in 100L/80D blends in comparison with 100L/100D blends. An epitaxial crystallization between the homopolymer 100L and the stereocomplex 100L/80D has also been observed at certain compositions. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailLatent Bovine Herpesvirus 1 Infection in Calves Protected by Colostral Immunity
Lemaire, Mylène; Meyer, Gilles; Ernst, E. et al

in Veterinary Record : Journal of the British Veterinary Association (1995), 137(3), 70-1

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailHeterogeneity of sponastrime dysplasia: Delineation of a variant form with severe mental retardation
Verloes, Alain ULg; Misson, Jean-Paul ULg; Dubru, J. M. et al

in Clinical Dysmorphology (1995), 4(3), 208-215

We report a child with short stature, osteopenia with metaphyseal striations and severe mental retardation. This child shows radiological and clinical features of SPONASTRIME dysplasia. Only three ... [more ▼]

We report a child with short stature, osteopenia with metaphyseal striations and severe mental retardation. This child shows radiological and clinical features of SPONASTRIME dysplasia. Only three sibships with this disorder have been reported. In two families, affected patients were of normal intelligence. In the third one, as well as our case, the dysplasia was complicated by severe mental retardation of unknown origin. The severity of the retardation in our case and a previous report, and some difference in the gestalt and radiological aspects, suggest that SPONASTRIME dysplasia is a heterogeneous disorder. We provisionally propose to split SPONASTRIME dysplasia in two phenotypically distinct subgroups, and to delineate here a 'new' variant with microcephaly and severe mental impairment. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailPolylactide microparticles prepared by double emulsion-evaporation 2 : Effect of the poly(lactide-co-glycolide) composition on the stability of the primary and secondary emulsions
Nihant, Nicole; Schugens, Chantal; Grandfils, Christian ULg et al

in Journal of Colloid and Interface Science (1995), 173(1), 55-65

Polylactide and copolymers with glycolide have been used as coating polymers in the microencapsulation technique based on the W1/o/w2 double emulsion-evaporation method. Stability of the primary emulsion ... [more ▼]

Polylactide and copolymers with glycolide have been used as coating polymers in the microencapsulation technique based on the W1/o/w2 double emulsion-evaporation method. Stability of the primary emulsion is of critical importance and clearly predetermines the internal morphology of the microspheres, in agreement with the very fast hardening of the oil phase (CH2Cl2) as soon as the primary emulsion is dispersed in water. When the peptide or' protein to be encapsulated is able to interact with the coating polymer and possibly with the surfactant, the stabilization mechanism of the primary emulsion is very complex. It has been shown that the interaction of BSA (bovine serum albumin) with the polyester is as strong as the glycolide content is high, which results in the formation of a solid film at the w1/o interface and in a high emulsion stability. Addition of a surfactant, such as poloxamer 188, in the oil phase has a deleterious effect on both the emulsion stability and the internal structure of the microspheres. Use of a dye tracer in the internal aqueous phase was not useful in probing the double emulsion stability and the encapsulation efficiency, because of specific interactions between the dye (indigocarmine) and the coating polyester. [less ▲]

Detailed reference viewed: 115 (8 ULg)
Full Text
Peer Reviewed
See detailControl of living anionic polymerization of (meth)acrylates : active complex species, macromolecular engineering and possible scaling-up
Teyssié, Philippe; Bayard, Philippe; Jérôme, Robert ULg et al

in Macromolecular Symposia (1995), 98

Tailored polymerization of acrylic and methacrylic esters, particularly methyl methacrylate (MMA), has been the topic of a frantic research effort during the last decade. It is the purpose of this paper ... [more ▼]

Tailored polymerization of acrylic and methacrylic esters, particularly methyl methacrylate (MMA), has been the topic of a frantic research effort during the last decade. It is the purpose of this paper to present one of the main lines of that quest, and to evaluate its potential versus other new approaches. [less ▲]

Detailed reference viewed: 47 (2 ULg)
Full Text
Peer Reviewed
See detailThe gene encoding bovine pregnancy-associated glycoprotein-1, an inactive member of the aspartic proteinase family
Xie, S.; Green, J.; Beckers, Jean-François ULg et al

in Gene (1995), 159(2), 193-197

Bovine pregnancy-associated glycoprotein 1 (bPAG1) is a member of the aspartic proteinase family. It becomes detectable in maternal serum soon after implantation and is produced specifically in the ... [more ▼]

Bovine pregnancy-associated glycoprotein 1 (bPAG1) is a member of the aspartic proteinase family. It becomes detectable in maternal serum soon after implantation and is produced specifically in the invasive binucleate cells of the placenta. As a result of a key mutation within its catalytic center, bPAG1 appears to be proteolytically inactive. Its gene consists of nine exons (size range 99-281 bp) and eight introns (87-1800 bp) organized in a manner very similar to those of proteolytically active mammalian aspartic proteinases. The transcription start point (tsp) is located 53 or 54 bp upstream from the start codon (ATG) and 19 bp downstream from a 5'-TATATAA sequence. Southern blot analyses have indicated the presence of two bPAG1 genes. By screening with an antiserum raised against bPAG1, a less common cDNA with 91% sequence identity to the bPAG1 transcript has been isolated from a placental cDNA library and presumably represents the second gene. At least eight other genes with sequences that hybridize relatively weakly to the bPAG1 probe are present in the bovine genome. Despite the similarities in the transcribed portion of the genes encoding PAG1, pepsinogen and other mammalian aspartic proteinases, the sequences upstream from the tsp of bPAG1 are unique. [less ▲]

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailSubstituent effect in anionic polymerization of β-lactones initiated by alkali metal alkoxides
Kurcok, Piotr; Matuszowicz, Andrzej; Jedlinski, Zbigniew et al

in Macromolecular Rapid Communications (1995), 16(7), 513-519

The influence of methyl substituent on the mechanism of the ring-opening polymerization of -lactones initiated by alkali metal alkoxides is discussed. Attention has been paid to the effect of the ... [more ▼]

The influence of methyl substituent on the mechanism of the ring-opening polymerization of -lactones initiated by alkali metal alkoxides is discussed. Attention has been paid to the effect of the substituent position in the monomer molecule on the ring-opening mechanism, the 3,3-dimethyl-2-oxetanone (pivalolactone), 4-methyl-2-oxetanone (beta-butyrolactone) and 2-oxetanone (beta-propiolactone) being chosen as model monomers. Moreover, it was found unexpectedly that in the case of pivalolactone polymerization, besides open-chain polymers, cyclic oligomers are produced. [less ▲]

Detailed reference viewed: 127 (3 ULg)