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See detailMitochondrial metabolite carrier family, topology, structure and functional properties: an overview.
Sluse, Francis ULg

in Acta Biochimica Polonica. Polish. (1996), 43(2), 349-360

A set of metabolite carriers operates the traffic of numerous molecules consumed or produced in mitochondrial matrix and/or cytosolic compartments. As their existence has been predicted by the ... [more ▼]

A set of metabolite carriers operates the traffic of numerous molecules consumed or produced in mitochondrial matrix and/or cytosolic compartments. As their existence has been predicted by the chemiosmotic theory, the first challenge, in the late sixties, was to prove their presence in the inner mitochondrial membrane and to describe the various transports carried out. The second challenge was to understand their mechanisms by the kinetic approach in intact mitochondria (seventies). The third challenge (late seventies-eighties) was to isolate and to reconstitute the carriers in liposomes in order to characterize the proteins and to establish the concept of a structural and a functional family as well as some structure-function relationship with the help of primary sequences. Genetics, molecular biology and genomic sequencing bring the fourth challenge (nineties): a raising number of putative carriers becomes known only by their primary sequences but their functions have to be discovered. The actual challenge of the future is the elucidation of the ternary structure of carrier proteins that together with site-directed mutagenesis and kinetic mechanism will permit to advance in the understanding of molecular mechanisms of transport processes. [less ▲]

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See detailOscillator strengths for Ca-like iron, cobalt and nickel (Fe VII, Co VIII, Ni IX)
Quinet, Pascal ULg; Hansen, J E

in Journal of Physics : B Atomic Molecular & Optical Physics (1996), 29

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See detailProduction of sulfur from gypsum as an industrial byproduct
Hiligsmann, Serge ULg; Deswaef, Sophie; Taillieu, Xavier et al

in Applied Biochemistry and Biotechnology (1996), 57-8

Biological sulfate reduction was investigated at the bench and pilot scales in order to determine optimum culture conditions. Efficient strains of sulfate-reducing bacteria (SRB) were selected by ... [more ▼]

Biological sulfate reduction was investigated at the bench and pilot scales in order to determine optimum culture conditions. Efficient strains of sulfate-reducing bacteria (SRB) were selected by classical microbiological methods and by mutagenesis. Improvement factors, including stripping, scale-up, sulfate,and organic substrate concentrations, have been studied in batch bioreactors. Two types of pilot-scale bioreactors have been adopted, the first being completely mixed with free cells and the second having two stages with immobilized cells on a fixed bed. An overall bioconversion capacity of 11 kg/m(3) . d of gypsum and 1.2 kg/m(3) . d of dissolved organic carbon has been achieved in the two-stage bioreactor. [less ▲]

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See detailOne-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.
Hokke, Cornelis H; Zervosen, Astrid ULg; Elling, Lothar et al

in Glycoconjugate Journal (1996), 13(4), 687-92

The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely ... [more ▼]

The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was formed in a 2.2 mumol ml-1 yield starting from the acceptor GlcNAc beta 1-->O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which pays an important role in various biological and immunological processes. [less ▲]

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See detailInfections oculaires traumatiques et iatrogènes: prélèvements et rôle du laboratoire
Melin, Pierrette ULg

in Bulletin de la Société Belge d'Ophtalmologie (1996), 260

Ocular inflammations may be due to a variety of diseases, and microorganisms play a major role in both acute and chronic eye diseases. Treatment of serious infections needs a good microbiological ... [more ▼]

Ocular inflammations may be due to a variety of diseases, and microorganisms play a major role in both acute and chronic eye diseases. Treatment of serious infections needs a good microbiological diagnostic. The indications and techniques for investigations are determined by the site of infection, severity of the process, and knowledge of the likely responsible organisms. Immediate inoculation of specimens on culture media in the examining room is often recommended. This article describes organisms associated with ocular infections, and is designed to assists ophthalmologists in the collection, processing and transport of specimens. [less ▲]

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See detailModelling the competition between two phytoplanktonic species
Elkafazi, Abderahman; Djenidi, Salim ULg; Nihoul, Jacques ULg

in Bulletin de la Société Royale des Sciences de Liège (1996), 65

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See detailRole of the Coulomb interaction on the ferroelectric instability of Barium Titanate
Ghosez, Philippe ULg; Gonze, X.; Michenaud, J. P.

in Ferroelectrics (1996), 186

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See detailStorm surges in the Arabian/Persian Gulf
Djenidi, Salim ULg; Neuberg, Luc

in Bulletin de la Société Royale des Sciences de Liège (1996), 65

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See detailThe influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
Bosseloir, A.; Bouzahzah, F.; Defrance, T. H. et al

in Scandinavian Journal of Immunology (1996), 43(1), 23-30

Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of ... [more ▼]

Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered. [less ▲]

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See detailA Non-Cofactor Role of Thiamine Derivatives in Excitable Cells?
Bettendorff, Lucien ULg

in Archives of Physiology & Biochemistry (1996), 104(6), 745-751

Thiamine diphosphate (TDP) is an important cofactor of pyruvate (PDH) and alpha-ketoglutarate (KGDH) dehydrogenases and transketolase. Thiamine deficiency leads to reversible and irreversible brain ... [more ▼]

Thiamine diphosphate (TDP) is an important cofactor of pyruvate (PDH) and alpha-ketoglutarate (KGDH) dehydrogenases and transketolase. Thiamine deficiency leads to reversible and irreversible brain lesions due to impaired oxidative metabolism. A specific non-cofactor role for thiamine has also been proposed in excitable cells and thiamine triphosphate (TTP) might be involved in the regulation of ion channels. Thiamine is taken up by neuroblastoma cells through a high affinity transporter. Inside the cells, it is rapidly phosphorylated to TDP. This high turnover TDP pool is the precursor for TTP. Most of the TDP however has a low turnover and is associated with PDH and KGDH in mitochondria. In excised inside-out patches from neuroblastoma cells, TTP, at a concentration of 1 microM, activates chloride channels of large unitary conductance, the so-called maxi-Cl- channels. These channels are inhibited by oxythiamine from the outide. In addition to the role of TTP in the regulation of chloride channels, thiamine itself, or a presently unknown analog, may have trophic effects on neuronal cells. [less ▲]

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See detailCytokine gene polymorphisms in inflammatory bowel disease.
Louis, Edouard ULg; Satsangi, J.; Roussomoustakaki, M. et al

in Gut (1996), 39(5), 705-10

BACKGROUND: Concordance rates in siblings and twins provide strong evidence that genetic susceptibility is important in the pathogenesis of inflammatory bowel disease. The number and identity of ... [more ▼]

BACKGROUND: Concordance rates in siblings and twins provide strong evidence that genetic susceptibility is important in the pathogenesis of inflammatory bowel disease. The number and identity of susceptibility genes is largely uncertain. Cytokine genes are attractive candidate loci. AIMS: To study allelic frequencies of polymorphisms of the interleukin-1 receptor antagonist (IL-1RA) gene and the tumour necrosis factor alpha gene in patients with inflammatory bowel disease. SUBJECTS: One hundred and twenty nine North European caucasoid patients with ulcerative colitis, 120 patients with Crohn's disease, and 89 healthy controls. METHODS: Genotyping was performed by polymerase chain reaction. A variable number of tandem repeats (VNTR) in the IL-1RA gene and a single base pair polymorphism in the TNF alpha gene promoter region (TNF-308) were analysed. RESULTS: No significant differences in IL-1RA VNTR allelic frequencies were noted between Crohn's disease (allele 1: 72.6%, allele 2: 24.7%, allele 3: 2.6%), ulcerative colitis (72.6%, 24.3%, 3.1%, respectively), and controls (76.9%, 20.8% and 2.3%). Some 42.4% of patients with ulcerative colitis and 43.4% patients with Crohn's disease were carriers of allele 2, compared with 34.8% healthy subjects. The TNF2 allele was modestly reduced in Crohn's disease (13.2%), compared with healthy subjects (21.3%; p = 0.04), and ulcerative colitis (21.6%). CONCLUSIONS: The associations demonstrated are modest: these polymorphisms are unlikely to be important determinants of overall disease susceptibility. [less ▲]

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See detailIsolation of nucleoli from ELT cells: a quick new method that preserves morphological integrity and high transcriptional activity.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1996), 228(1), 125-31

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to ... [more ▼]

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to empty one of the three major compartments of the nucleolus, the fibrillar center, of its content. We have used the AgNOR staining and in vitro transcription assay to test the degree of structural and functional preservation of the isolated nucleoli. Our results demonstrate the value of our procedure as a reliable tool for biochemical and ultrastructural studies on the nucleolus. Moreover, these proprieties prompt us to investigate the rRNA synthesis, using a nonisotopic approach, within morphologically intact isolated nucleoli. Thus, we show that newly synthesized rRNA transcripts are located not only in the dense fibrillar component, but also indubitably in the fibrillar center. [less ▲]

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See detailLa Biologie clinique en pédiatrie: hypotonie et retard moteur
Misson, Jean-Paul ULg; Leroy, Patricia; Dubru, Jean-Marie ULg et al

in Acta Paediatrica Belgica (1996), 28(2), 95

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See detailHeparin-coated Palmaz-Schatz stents in human coronary arteries. Early outcome of the Benestent-II Pilot Study.
Serruys, P. W.; Emanuelsson, H.; van der Giessen, W. et al

in Circulation (1996), 93(3), 412-22

BACKGROUND: The purpose of the Benestent-II Pilot Study was to evaluate the safety of delaying and eliminating anticoagulant therapy in patients receiving a heparin-coated stent in conjunction with ... [more ▼]

BACKGROUND: The purpose of the Benestent-II Pilot Study was to evaluate the safety of delaying and eliminating anticoagulant therapy in patients receiving a heparin-coated stent in conjunction with antiplatelet drugs. METHODS AND RESULTS: The study consisted of three initial phases (I, II, III) during which resumption of heparin therapy after sheath removal was progressively deferred by 6, 12, and 36 hours. In phase IV, coumadin and heparin were replaced by 250 mg ticlopidine and 100 mg aspirin. Of the 207 patients with stable angina pectoris and a de novo lesion in whom heparin-coated stent implantation was attempted, implantation was successful in 202 patients (98%). Stent thrombosis did not occur during all four phases, and the overall clinical success rate at discharge was 99%. Bleeding complications requiring blood transfusion or surgery fell from 7.9% in phase I to 5.9%, 4%, and 0% in the three following phases. Hospital stay was 7.4, 6.1, 7.2, and 3.1 days for the consecutive phases. The restenosis rate for the combined four phases was 13% (15% in phase I, 20% in phase II, 11% in phase III, and 6% in phase IV). The overall rate of reintervention for the four phases was 8.9%. At 6 months, 84%, 75%, 94%, and 92% of the patients of phases I to IV, respectively, were event free. For the four phases, the event-free rate was 86%, which compares favorably with the rate observed in the Benestent-I study (80%; relative risk, 0.68 [0.45 to 1.04]). CONCLUSIONS: The implantation of stents coated with polyamine and end-point-attached heparin in stable patients with one significant de novo coronary lesion is well tolerated, is associated with no (sub)acute stent thrombosis, and results in a favorable event-free survival after 6 months. [less ▲]

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See detail8-Chloro-5-(4-methylpiperazin-1-yl)-11H-pyrido[2,3-b][1,5]benzodiazepine
Dupont, L.; Liégeois, Jean-François ULg; Rogister, F. et al

in Acta Crystallographica Section C-Crystal Structure Communications (1996), C52

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See detailCrystallization and preliminary X-ray diffraction studies of a-amylase from the antarctic psychrophile Alteromonas haloplanctis A23
Aghajari, N.; Feller, Georges ULg; Gerday, Charles ULg et al

in Protein Science : A Publication of the Protein Society (1996), 5(10), 2128-2129

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the ... [more ▼]

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-Amylase from A. haloplanctis, which shares 53% sequence identity with pig pancreatic alpha-amylase, has been crystallized and data to 1.85 A have been collected. The space group is found to be C222(1) with a = 71.40 A, b = 138.88 A, and c = 115.66 A. Until now, a three-dimensional structure of a psychrophilic enzyme is lacking. [less ▲]

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See detailLe vétérinaire et la qualité microbiologique des denrées alimentaires d’origine animale
Vindevogel, Henri ULg; Daube, Georges ULg; Denys, Jacques et al

in Annales de Médecine Vétérinaire (1996), 141

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See detailCalcitonin
Reginster, Jean-Yves ULg

in Current Opinion in Orthopaedics (1996), 7

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See detailComparaison des performances de porcs élevés sur litières ou sur caillebotis
Nicks, Baudouin ULg; Desiron, Alain; Canart, Bernard et al

in Annales de Médecine Vétérinaire (1996), 140

In a first experiment, performances and carcass quality of fattening pigs kept either on a litter with sawdust, on straw or on slatted floor were compared. Three batches of 17 pigs were kept on each floor ... [more ▼]

In a first experiment, performances and carcass quality of fattening pigs kept either on a litter with sawdust, on straw or on slatted floor were compared. Three batches of 17 pigs were kept on each floor type. Pens with litter were not cleaned until the end of the third fattening period. On average 100 kg of sawdust or 49 kg of straw were used per pig. The growth rate of pigs on litters was higher (P < 0,01) than that of pigs on slatted floor : 739 g/d on sawdust, 745 g/d on straw and 686 g/d on slatted floor. Carcass quality at slaughter was not different according to the floor type. The peak shear force of the M. Longissimus dorsi was higher for pigs kept on straw than for the 2 other groups. In a second experiment, 97 weaners, 4 weeks old, were divided in 3 groups on 3 floor types : a deep litter with sawdust, a deep litter with sawdust mixed with straw and a slatted floor. Between 4 and 10 weeks of age, the growth rates of pigs were different (P < 0,05) according to the 2 litters : 438 g/d on the deep litter with straw and sawdust, 397 g/d on sawdust and higher (P < 0,001) than that on slatted floor (273 g/d). Pigs on slatted floor were however disadvantaged by a lower health status in the breeding pig house. [less ▲]

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See detailA proposito de un indice de egocentricidad (Exner) bajo
Mormont, Christian ULg

in Revista de la Sociedad Espanola del Rorschach y Metodos Proyectivos (1996), 9

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See detailMaurice Streel, an annotated list of his publications.
Steemans, Philippe ULg; Loboziak, S

in Annales de la Société Géologique de Belgique (1996), 117

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See detailEnergy levels and oscillator strengths for transitions in Pd II
Quinet, Pascal ULg

in Physica Scripta (1996), 54

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See detailReturns Interval and Variability in Risk Measurement
Corhay, Albert ULg; Tourani Rad, Alireza

in Belgian Journal of Operations Research, Statistics and Computer Science = Revue Belge de Recherche Opérationnelle, de Statistique et d'Information (1996), 36

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See detailPremière approche de la flore macrolichénique corticole de la Région de Bruxelles-Capitale en situation de dépollution au SO2
Tanghe, Martin; Richel, Thierry; Crisan, Florin et al

in Belgian Journal of Botany (1996), 129

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See detailDeficient Habituation of Evoked Cortical Potentials in Migraine: A Link between Brain Biology, Behavior and Trigeminovascular Activation?
Schoenen, Jean ULg

in Biomedicine & Pharmacotherapy (1996), 50(2), 71-8

According to recent evoked potential studies, a fundamental, probably protective, feature of cortical information processing, ie, response habituation during stimulus repetition, is abnormal in migraine ... [more ▼]

According to recent evoked potential studies, a fundamental, probably protective, feature of cortical information processing, ie, response habituation during stimulus repetition, is abnormal in migraine between attacks. The deficient habituation is found for different sensory modalities and experimental paradigms: pattern-reversal visual evoked potentials (same stimulus at a constant intensity), cortical auditory evoked potentials (same stimulus at increasing intensities) and auditory event-related potentials obtained in a passive "oddball" paradigm (novel stimulus). The abnormal information processing is an interictal cortical dysfunction most likely due to inadequate control by the so-called "state-setting, chemically-addressed pathways" originating in the brain stem, in particular by the serotonergic pathway, leading to a low preactivation level of sensory cortices. We suggest that it may play a pivotal role in migraine pathogenesis in conjunction with the reported decrease of brain mitochondrial energy reserve, by favouring a rupture of metabolic homeostasis and biochemical shifts capable of activating the trigeminovascular system and thus capable of producing a migraine attack. We postulate that both the deficient habituation in information processing and the deranged oxygen metabolism may have behavioral correlates. Which of these abnormalities are inherited, acquired or both remains to be determined. [less ▲]

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See detailDémarche préventive d'une école du dos
Vanderthommen, Marc ULg; Drosson, C.; TOMASELLA, Marco ULg et al

in Kine 2000 (1996), 5(7),

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See detailVariations saisonnières du CO2 de l'air de deux grottes du Jura méridional: La Balme et Cerdon. Comparaison avec d'autres grottes françaises
Sbai, Abdelkader; Ek, Camille ULg; Renault, Philippe

in Annales de la Société Géologique de Belgique (1996), 119

Seasonal measurements of carbon dioxide were made over a period of one year in two caves from southern Jura: the caves of La Balme and of Cerdon. Carbon dioxide contents range from 300 to 15,500 ppm ... [more ▼]

Seasonal measurements of carbon dioxide were made over a period of one year in two caves from southern Jura: the caves of La Balme and of Cerdon. Carbon dioxide contents range from 300 to 15,500 ppm; these low values are correlated with the circulation of the air in the two caves and with the origin of the carbon dioxide. The values found in these caves are then compared with those measured by researchers in other French caves. [less ▲]

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See detailImprint and Brush Cytology in the Diagnosis of Canine Intranasal Tumours
Clercx, Cécile ULg; Wallon, J.; Gilbert, S. et al

in Journal of Small Animal Practice (1996), 37(9), 423-7

Fifty-four dogs with nasal tumours were included in this study. Based on histopathology 52 tumours were malignant (36 epithelial and 16 mesenchymal) and two were benign (one oncocytoma and one ... [more ▼]

Fifty-four dogs with nasal tumours were included in this study. Based on histopathology 52 tumours were malignant (36 epithelial and 16 mesenchymal) and two were benign (one oncocytoma and one pleiomorphic adenoma). Malignancy was significantly more frequently diagnosed by imprint cytology (81 per cent of the cases) than by brush cytology (56 per cent). Brush cytology was a significantly more sensitive technique in epithelial than in mesenchymal tumours, while the sensitivity of imprint cytology was not affected by the histological type. Brush cytology determined an epithelial origin in 88 per cent of epithelial tumours, and imprint cytology in 90 per cent. In mesenchymal tumours, the scores were significantly lower, the histological type being determined in only 20 per cent and 50 per cent, using brush and imprint cytology, respectively. [less ▲]

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See detailDifferential effects of gonadotropin-releasing hormone, dopamine and somatostatin and their second messengers on the mRNA levels of gonadotropin IIb subunit and growth hormone in the teleost fish, tilapia
Melamed, P.; Gur, G.; Elizur, A. et al

in Neuroendocrinology (1996)

In cultured pituitary cells of tilapia, gonadotropin-releasing hormone (GnRH; 10 nM 4-24 h), elevation of cyclic AMP (by 10 microM forskolin or 0.2 mM 3-isobutyl-1-methylxanthine: IBMX 0.5-36 h) or ... [more ▼]

In cultured pituitary cells of tilapia, gonadotropin-releasing hormone (GnRH; 10 nM 4-24 h), elevation of cyclic AMP (by 10 microM forskolin or 0.2 mM 3-isobutyl-1-methylxanthine: IBMX 0.5-36 h) or activation of protein kinase C (PKC; by 12.5 nM tetradecanoyl phorbol-13-acetate: TPA, 0.5-24 h) all increased gonadotropin (GtH) II beta steady state mRNA levels by three to four-fold. The involvement of PKA and PKC in the GnRH stimulatory effect on both GtH release and GtH II beta mRNA levels was corroborated by use of the PKA and PKC inhibitors, H89 and GF109203X, respectively (100 nM) which attenuated the GnRH effect. Incubation with actinomycin D (8 microM, 4-21 h) after preexposure for 24 h to either forskolin (10 microM) or TPA (12.5 nM), revealed that rates of transcript degradation were slower in forskolin-treated cells (T 1/2 = 14.1 h) than in control or TPA-treated cells (T 1/2 = 8.47 or 8.38 h), suggesting a stabilizing effect on the mRNA. Dopamine (DA; 10 microM, 4-36 h) had no apparent effect on steady state mRNA levels of GtH II beta, but reduced GtH release by as much as 75%. Steady state levels of growth hormone (GH) mRNA were not affected by exposure to GnRH (10 nM, 4-24 h), although GH release was more than doubled. Similarly, activation of PKC (by TPA 12.5 nM, 1.5-36 h), which was shown to be essential for the GnRH-stimulatory effect on GH release, did not alter levels of the GH transcript, but increased GH release by more than fivefold. DA (10 microM, 4-24 h) moderately increased GH transcript levels (160%) with similar kinetics but lower potency than direct elevation of cAMP (by 10 microM forskolin or 0.2 mM IBMX, 0.5-36 h) which increased transcript levels by more than fourfold. The involvement of PKA in the DA effect was confirmed when the PKA inhibitor H89 (100 nM, 15 min prior to DA exposure) attenuated the DA effect on GH mRNA levels. Exposure of cells to actinomycin D (8 microM, 2-16 h) after treatment with forskolin (10 microM, 24 h) led to a slower rate of transcript degradation than in control cells (T 1/2 = 6.5 h vs. T 1/2 = 4.36 h), suggesting that cAMP also elicits a stabilizing effect on GH mRNA. Somatostatin (100 nM, 0.5-36 h) had no clear effect on GH transcript levels, but reduced GH release by as much as 90%. These results suggest that activation of either cAMP-PKA or PKC pathways can, possibly by different mechanisms, stimulate mRNA levels of the GtH II beta gene, but that only the cAMP-PKA pathway stimulates GH mRNA levels. It would appear therefore that GnRH, although stimulating GH release, does not regulate GH transcription in this fish. [less ▲]

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See detailRecommendations for the registration of agents used in the prevention and treatment of glucocorticoid-induced osteoporosis
Compston, JE; Audran, M; Avouac, B et al

in Calcified Tissue International (1996), 59

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See detailMechanism of Single-Electron Capture by the Dichlorocarbene Dication.
Leyh, Bernard ULg; Hautot, D.

in Journal of the American Society for Mass Spectrometry (1996), 7

The single-electron capture (SEC) by dichlorocarbene with eight different atomic and molecular target gases, (CCl2)2++G->(CCl2)++G+, has been studied by product ion spectroscopy and ion kinetic energy ... [more ▼]

The single-electron capture (SEC) by dichlorocarbene with eight different atomic and molecular target gases, (CCl2)2++G->(CCl2)++G+, has been studied by product ion spectroscopy and ion kinetic energy spectroscopy. The experimental data have been interpreted in the framework of a theoretical model that describes the charge exchange process. Exothermic charge exchange is handled within the Landau-Zener model, whereas endothermic charge exchange is described by the Demkov model. The calculated data reproduce qualitatively the essential features of the experimental results: (1) the appearance of a reaction window centered at an exothermicity in the 4-4.5 eV range, (2) the lower SEC cross sections for endothermic charge exchange, (3) the wider internal energy distributions obtained for CCl2+ in the endothermic regime than in the exothermic one, which results in larger dissociation yields, (4) the excitation of molecular targets that accompany their ionization in the SEC process, and (5) the kinetic energy released on the CCl++Cl fragments in dissociative SEC. [less ▲]

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See detailMiospore ranges near the D/C Boundary and extinction events.
Streel, Maurice ULg

in Newsletter. Subcommission on Devonian Stratigraphy (1996), 12

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See detailSur le bilinguisme dans l'Egypte gréco-romaine
Rochette, Bruno ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1996), 71(141), 153-168

Grec et latin dans l'administration civile. Degré de connaissance du latin dans les milieux hellénophones de l'Égypte gréco-romaine. Grec, latin et égyptien (le bilinguisme de l'Égypte gréco-romaine ne ... [more ▼]

Grec et latin dans l'administration civile. Degré de connaissance du latin dans les milieux hellénophones de l'Égypte gréco-romaine. Grec, latin et égyptien (le bilinguisme de l'Égypte gréco-romaine ne concerne pas seulement le grec et le latin). [less ▲]

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See detailPhénomènes karstiques en milieux non carbonatés. Etudes de cavités et problématique de leur développement au Niger Occidental
Willems, Luc ULg; Pouclet, André; Lenoir, François et al

in Zeitschrift für Geomorphologie (1996)

The study of several caves in siliceous formations belonging to the Precambrian basement and its Cenozoic sedimentary cover brings to the fore the importante of an underground drainage network in relation ... [more ▼]

The study of several caves in siliceous formations belonging to the Precambrian basement and its Cenozoic sedimentary cover brings to the fore the importante of an underground drainage network in relation with crustal fracturation. This network guides the waler transfer from the land surface to the depth and generates géomorphologic phenomena similar to those in calcareous areas. The study of caves in a siliceous environmemts shows the importance of solution phenomena in their formation and also in that of West Niger landscapes. Therfore, a general model of topographic evolution îs proposed. [less ▲]

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See detailSuppression of Voluntary Temporalis Muscle Activity by Peripheral Limb Stimulations in Healthy Volunteers, Migraineurs and Tension-Type Headache Sufferes
Wang, W.; Schoenen, Jean ULg

in Functional Neurology (1996), 11(6, Nov-Dec), 307-15

We studied the inhibition of voluntary temporalis muscle activity after stimulations of extracephalic cutaneous or mixed nerves in 23 healthy volunteers, 29 patients suffering from migraine without aura ... [more ▼]

We studied the inhibition of voluntary temporalis muscle activity after stimulations of extracephalic cutaneous or mixed nerves in 23 healthy volunteers, 29 patients suffering from migraine without aura, 24 from episodic and 42 from chronic tension-type headache. Two successive EMG suppressions were induced bilaterally after stimulating at 40 mA the index finger or the median nerve, but not after stimulations of the sural or peroneal nerves. They differed from those obtained after labial commissure stimulation at 20 mA, i.e. the classic temporalis silent periods ES1 and ES2. Overall prevalence of index- (3%) and median nerve-induced (16%) early suppression was much lower than that of labial-induced ES1 (100%); prevalence of index-induced late suppression (index-"ES2") was 62% and that of median-"ES2" 48%, compared with 97% for labial-ES2. Latency and duration of index-/median-"ES2" were not significantly different between groups. Index-"ES2" occurred significantly more often in migraineurs (83%) than in controls (48%) suggesting that the spino-bulbar pathways involved in index-/median-"ES2" are hyperexcitable in migraine. [less ▲]

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See detailAbsolute configuration of (R)-1-phenylethyl ammonium (S)-2-(6-methoxy-2-naphthyl)-propionate
Dupont, L.; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Acta Crystallographica (1996), C52

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See detailPharmacological characterization of the enhancement of apomorphine-induced gnawing in mice by cocaine
Tirelli, Ezio ULg; Witkin, J. M.

in Pharmacology, Biochemistry & Behavior (1996), 55(1), 135-140

Characterized the behavioral and pharmacological mechanisms associated with cocaine potentiation of apomorphine (AP)-induced gnawing (AIG) in male C57BL/6J mice. Results showed that (-)-cocaine enhanced ... [more ▼]

Characterized the behavioral and pharmacological mechanisms associated with cocaine potentiation of apomorphine (AP)-induced gnawing (AIG) in male C57BL/6J mice. Results showed that (-)-cocaine enhanced AIG at doses devoid of effects on gnawing when given alone; (+)-cocaine or (-)-cocaine methiodide were also devoid of effects. Lidocaine, a local anesthetic without prominent dopaminergic actions, augmented the gnawing response to AP without increasing climbing or gnawing when given alone. (+)-Amphetamine enhanced AIG gnawing but only at a high dose that increased gnawing by itself. The selective dopamine (DA) uptake blocker, GBR 12909, augmented AIG by itself; however, it increased climbing at doses that augmented the gnawing response. These data indicate that the cocaine-augmented gnawing response to AP may be due to blockade of DA uptake and or the local anesthetic actions of cocaine. ((c) 1997 APA/PsycINFO, all rights reserved) [less ▲]

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See detailAlterations in the ultradian oscillations of insulin secretion and plasma glucose in aging.
Scheen, André ULg; Sturis, J.; Polonsky, K. S. et al

in Diabetologia (1996), 39(5), 564-72

Normal insulin secretion includes oscillations with a period length of 80-150 min which are tightly coupled to glucose oscillations of similar period. To determine whether normal aging is associated with ... [more ▼]

Normal insulin secretion includes oscillations with a period length of 80-150 min which are tightly coupled to glucose oscillations of similar period. To determine whether normal aging is associated with alterations in these ultradian oscillations, eight, modestly overweight, older men (65 +/- 5 years) and eight weight-matched young control subjects (25 +/- 4 years) were studied during 53 h of constant glucose infusion. Blood samples were collected every 20 min and insulin secretion rates were calculated by deconvolution. Ultradian oscillations of glucose and insulin secretion were evident in both groups. Pulse frequency was similar for glucose and insulin secretion, and was not affected by age. The absolute amplitude of the glucose oscillations was similar in both groups but their relative amplitude was slightly dampened in the older adults. Both the absolute and the relative amplitudes of insulin secretory oscillations were markedly reduced in the older subjects. The normal linear increase in the amplitude of insulin oscillations occurring with increasing amplitudes of glucose oscillations was still present in the older adults but analysis of covariance indicated that the slope was significantly lower than in the young control subjects (p < 0.0005), reflecting a decreased responsiveness of the beta cell to glucose changes. The temporal concordance between insulin and glucose oscillations, as estimated by pulse concomitancy and cross-correlation, was also lower in older subjects. The similarities between the alterations in the ultradian oscillations of insulin secretion and glucose in older healthy adults and those occurring in diabetic patients suggest that an impairment of beta-cell function may play a primary role in the deterioration of glucose tolerance in aging. [less ▲]

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See detailRheinische Wortgeographie. Karten des Rheinischen Wörterbuchs und ihre computative Auswertung. Teil I
Lausberg, Helmut; Möller, Robert ULg

in Rheinische Vierteljahrsblätter (1996), 60

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See detailImages liées à la pathologie traumatique du système nerveux central
Leroy, Patricia; Dubru, Jean-Marie ULg; Misson, Jean-Paul ULg

in Acta Paediatrica Belgica (1996), 28(3), 216

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See detailThe Coastal Ocean in a Global Change Perspective
Djenidi, Salim ULg

in Journal of Marine Systems (1996), 7(2-4), 117-118

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See detailClinical pharmacokinetics of metformin.
Scheen, André ULg

in Clinical Pharmacokinetics (1996), 30(5), 359-71

The biguanide metformin (dimethylbiguanide) is an oral antihyperglycaemic agent widely used in the management of non-insulin-dependent diabetes mellitus (NIDDM). Considerable renewal of interest in this ... [more ▼]

The biguanide metformin (dimethylbiguanide) is an oral antihyperglycaemic agent widely used in the management of non-insulin-dependent diabetes mellitus (NIDDM). Considerable renewal of interest in this drug has been observed in recent years. Metformin can be determined in biological fluids by various methods, mainly using high performance liquid chromatography, which allows pharmacokinetic studies in healthy volunteers and diabetic patients. Metformin disposition is apparently unaffected by the presence of diabetes and only slightly affected by the use of different oral formulations. Metformin has an absolute oral bioavailability of 40 to 60%, and gastrointestinal absorption is apparently complete within 6 hours of ingestion. An inverse relationship was observed between the dose ingested and the relative absorption with therapeutic doses ranging from 0.5 to 1.5 g, suggesting the involvement of an active, saturable absorption process. Metformin is rapidly distributed following absorption and does not bind to plasma proteins. No metabolites or conjugates of metformin have been identified. The absence of liver metabolism clearly differentiates the pharmacokinetics of metformin from that of other biguanides, such as phenformin. Metformin undergoes renal excretion and has a mean plasma elimination half-life after oral administration of between 4.0 and 8.7 hours. This elimination is prolonged in patients with renal impairment and correlates with creatinine clearance. There are only scarce data on the relationship between plasma metformin concentrations and metabolic effects. Therapeutic levels may be 0.5 to 1.0 mg/L in the fasting state and 1 to 2 mg/L after a meal, but monitoring has little clinical value except when lactic acidosis is suspected or present. Indeed, when lactic acidosis occurs in metformin-treated patients, early determination of the metformin plasma concentration appears to be the best criterion for assessing the involvement of the drug in this acute condition. After confirmation of the diagnosis, treatment should rapidly involve forced diuresis or haemodialysis, both of which favour rapid elimination of the drug. Although serious, lactic acidosis due to metformin is rare and may be minimised by strict adherence to prescribing guidelines and contraindications, particularly the presence of renal failure. Finally, only very few drug interactions have been described with metformin in healthy volunteers. Plasma levels may be reduced by guar gum and alpha-glucosidase inhibitors and increased by cimetidine, but no data are yet available in the diabetic population. [less ▲]

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See detailSeduzione borghese e cultura dell'artificio. Femmes fatales in Oriani e Chelli
Curreri, Luciano ULg

in Italian Quarterly (1996), 129-130

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See detailActivation of ATP-dependant K+ channels and inhibition of insulin release: effect of BPDZ 62
Lebrun, P.; Antoine, M.-H.; Ouedraogo, R. et al

in Journal of Pharmacology and Experimental Therapeutics (The) (1996), 277

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See detailPolar overdominance at the ovine callipyge locus
Cockett, N.; Jackson, S.; Shaw, T. et al

in Science (1996), 273(5272), 236-238

An inheritable muscular hypertrophy was recently described in sheep and shown to be determined by the callipyge gene mapped to ovine chromosome 18. Here, the callipyge phenotype was found to be ... [more ▼]

An inheritable muscular hypertrophy was recently described in sheep and shown to be determined by the callipyge gene mapped to ovine chromosome 18. Here, the callipyge phenotype was found to be characterized by a nonmendelian inheritance pattern, referred to as polar overdominance, where only heterozygous individuals having inherited the callipyge mutation from their sire express the phenotype. The possible role of parental imprinting in the determinism of polar overdominance is envisaged. [less ▲]

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See detailChanges in breath 13CO2/12CO2 during exercise of different intensities.
Gautier, J. F.; Pirnay, Freddy ULg; Lacroix, M. et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1996), 81(3), 1096-102

The measurement of breath 13CO2/12CO2 is commonly used during exercise to evaluate the oxidation rate of exogenous carbohydrates enriched in 13C. The aim of this study was to investigate whether exercise ... [more ▼]

The measurement of breath 13CO2/12CO2 is commonly used during exercise to evaluate the oxidation rate of exogenous carbohydrates enriched in 13C. The aim of this study was to investigate whether exercise itself affects the 13C/12C ratio in expired air CO2 in relation to exercise intensity. The relative abundance of 13C and 12C in expired air CO2 was determined by isotoperatio mass spectrometry and expressed as delta 13C (in %o) by using Craig's formula and calibrated standards. Five healthy young men exercised on a treadmill after an overnight fast during > or = 105 min on four occasions and in a randomized order. Work rates were performed at approximately 30, 45, 60, and 75% of their maximal O2 uptake (VO2max). Delta 13C in expired air CO2 and respiratory exchange ratio (RER) were determined every 15 or 30 min during exercise. At 30 and 45% VO2max, a slight and not statistically significant increase in delta 13C was observed at 30 min. In contrast, at 60 75% VO2max, the rise was statistically significant and averaged 0.83 and 0.99%o, respectively. Average delta 13C (between 0 and 105 min) progressively increased with the intensity of exercise. Individual values of delta 13C and RER were positively correlated (r = 0.653, P = 0.002) as were values of delta 13C and endogenous carbohydrates utilized (r = 0.752, P < 0.001). Factitious or "pseudooxidation" of a 13C-enriched exogenous glucose load (indeed noningested) was calculated from the changes in expired air delta 13C. Over the whole period of exercise it was not statistically significant at 30 and 40% VO2max. However, over the first 60 min of exercise, such pseudooxidation of exogenous glucose was significant at 30 and 45% VO2max. In conclusion, by modifying the mix of endogenous substrates oxidized, exercise at 60% VO2max and above significantly increases the 13C/12C ratio in expired air CO2. At these intensities, this could lead to overestimation of the oxidation of 13C-labeled substrates given orally. At lower intensities of exercise, such overestimation is much smaller an affects mainly the values recorded during the initial part of the exercise bout. [less ▲]

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See detailCodon adjustment to maximise heterologous gene expression in Streptomyces lividans can lead to decreased mRNA stability and protein yield.
Lammertyn, E; Van Mellaert, L; Bijnens, A P et al

in Molecular & General Genetics [=MGG] (1996), 250(2), 223-9

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the ... [more ▼]

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the mTNF gene were therefore adapted to the codon bias of Streptomyces by site-directed mutagenesis. No improvement in mTNF yield could be detected. The stability of the transcript derived from the construct was shown to be more important for determining the final level of mTNF production. A strong correlation was observed between the yield of secreted biologically active mTNF and the amount of mTNF mRNA present in the cells. [less ▲]

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See detailFirst synthesis of 4-substituted benzenesulfonylcyanoguanidines
Masereel, B.; Lebrun, P.; Dogne, J.-M. et al

in Tetrahedron Letters (1996), 37

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See detailAssessment of nuclear totipotency of fetal bovine diploid germ cells by nuclear transfer.
Moens, A.; Chesne, P.; Delhaise, F. et al

in Theriogenology (1996), 46(5), 871-80

Nuclear transfer was used to study nuclear reprogramming of fetal diploid bovine germ cells collected at two stages of the fetal development. In the first case, germ cells of both sexes were collected ... [more ▼]

Nuclear transfer was used to study nuclear reprogramming of fetal diploid bovine germ cells collected at two stages of the fetal development. In the first case, germ cells of both sexes were collected during their period of intragonadal mitotic multiplication at 48 days post coitum (d.p.c.). In the second case, only male germ cells were collected after this period, between 105 and 185 d.p.c. Isolated germ cells were fused with enucleated oocytes. Reconstituted embryos were cultured in vitro and those reaching the compacted morula or blastocyst stage were transferred into synchronous recipient heifers. Of 511 reconstituted embryos with 48 d.p.c. germ cells (309 males and 202 females), 48% (247/511 ) cleaved; 2.7% (14/511 ) reached the compacted morula stage and 8 of them the blastocyst stage (1.6%). No difference was observed between sexes. All 14 compacted morulae/blastocysts were transferred into 6 recipients and one pregnancy was initiated. This recipient was slaughtered at Day 35 and an abnormal conceptus (extended trophectoderm and degenerated embryo) was collected. Its male sex, genetically determined, corresponded to that of donor fetus. Of 380 reconstituted embryos with male 105 to 185 d.p.c. germ cells, 72.1% (274/380 ) cleaved, 2.1% (8 380 ) reached the compact morula stage and 7 of these the blastocyst stage (1.8%). Three blastocysts and one morula were transferred into 4 recipients. Two became pregnant at Day 21 but only one at Day 35 which aborted around Day 40. Our results show that the nucleus of diploid bovine germ cells of both sexes can be reprogrammed. However, in the absence of further development of these reconstituted embryos, nuclear totipotency of bovine diploid germ cells remains to be evidenced. [less ▲]

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See detailOpportunities for using two low-cost methods for treatment of metal bearing aqueous streams
Gaydardzhiev, Stoyan ULg; Hadjihristova, M.; Tichy, R.

in Minerals Engineering (1996), 9

The feasibility of two non-traditional approaches - reedbeds as a natural wetland and adsorption on lignite and its maceral fractions - for their application in metal bearing effluent treatment is ... [more ▼]

The feasibility of two non-traditional approaches - reedbeds as a natural wetland and adsorption on lignite and its maceral fractions - for their application in metal bearing effluent treatment is discussed. The main mechanisms of metals uptake are otulined. The preliminary results from a pilot scale wetland operation for metallurgical effluents purification are analysed. For the second approach - the different maceral fractions derived from coal refining have shown different metal uptake apptitude. Zeta potential studies and adsorption isotherms have explained these findings. [less ▲]

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See detailThe inducible 70,000 molecular/weight heat shock protein is expressed in the degenerating dentate hilus and piriform cortex after systemic administration of kainic acid in the rat.
Armstrong, J. N.; Plumier, Jean-Christophe ULg; Robertson, H. A. et al

in Neuroscience (1996), 74(3), 685-93

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic ... [more ▼]

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic administration of kainic acid. In contrast to previous reports, the present study demonstrates that neurons in the regions most susceptible to seizure-induced cell death accumulate both Hsp70 messenger RNA and protein. Neurons in the denate hilus and piriform cortex contained Hsp70 messenger RNA at 6 h and protein at 12 h. These neutrons contained little or no Hsp70 messenger RNA or protein at 24 h when the majority of cells in these area were pyknotic. Injured neurons in areas such as the parietal cortex, which are less susceptible to seizure-induced cell death, expressed and maintained high levels of Hsp70 messenger RNA and protein at 12 and 24 h. This work suggest that Hsp70 messenger RNA and protein are rapidly and transiently expressed in dying neurons, and contradicts the notion that Hsp70 only accumulates in injured neurons that survive. [less ▲]

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See detailFinite element modelling of the competition between shear bands in the early stage of thrusting : Strain localisation and constitutive law influence
Barnichon, Jean-Dominique; Charlier, Robert ULg

in Special Publication - Geological Society of London (1996), 99

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See detailNew bisphosphonates for the treatment of osteoporosis : concerns and hopes
Reginster, Jean-Yves ULg

in Revue du Rhumatisme (English ed.) (1996), 63(7-8), 461-464

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See detailAssessment of residual insulin secretion in diabetic patients using the intravenous glucagon stimulatory test: methodological aspects and clinical applications.
Scheen, André ULg; Castillo, M. J.; Lefebvre, Pierre ULg

in Diabètes & Métabolism (1996), 22(6), 397-406

Defective insulin secretion plays a crucial role in insulin-dependent (Type 1) and non-insulin-dependent (Type 2) diabetes mellitus as well as in many secondary forms of the disease. Glucagon is a potent ... [more ▼]

Defective insulin secretion plays a crucial role in insulin-dependent (Type 1) and non-insulin-dependent (Type 2) diabetes mellitus as well as in many secondary forms of the disease. Glucagon is a potent stimulus for the islet beta-cell, and intravenous bolus injection of 1 mg glucagon has been widely used to assess endogenous insulin secretion for clinical or research purposes. Plasma C-peptide levels (less commonly insulin) are usually measured immediately before and 6 min after glucagon injection. The C-peptide response to glucagon is well-correlated with the beta-cell response to mixed meals or other stimuli commonly used to characterize endogenous insulin secretion (oral or intravenous glucose, standard meals, arginine, etc.) and has the advantage of shorter duration and simple standardization. The glucagon test shows good intra-subject reproducibility, although in diabetic patients it may be influenced by variable prevailing blood glucose levels. Several applications of the glucagon test have been developed. In Type 1 diabetes, the glucagon test has been used to discriminate between patients with and without residual insulin secretion. This can be especially important during the first few months, or even years, following initiation of insulin therapy when attempts to stop the immunological destruction of the beta-cell are made. Assessment of endogenous insulin secretion is also important after pancreas or islet transplantation. In patients with Type 2 diabetes mellitus, in which residual endogenous insulin secretion is common, characterization of the disease may help in the choice of therapy for the individual patient (insulin, sulphonylureas or combined therapy). Thus, the glucagon test is a simple, reliable and useful tool for clinical evaluation of diabetes mellitus. [less ▲]

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See detailDifferential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury.
Plumier, Jean-Christophe ULg; Robertson, H. A.; Currie, R. W.

in Journal of Molecular and Cellular Cardiology (1996), 28(6), 1251-60

Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer ... [more ▼]

Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer-perfused isolated rat heart after 30 min of coronary artery occlusion. The accumulation of mRNA for hsc70, hsp70, c-fos, c-jun, and Erg-1 was localized coincidently and was restricted to the ischaemic area of the heart. mRNA for these genes was undetectable at the end of the ischaemic period (no reperfusion). After 30 min of reperfusion, accumulation of mRNA for hsc70, hsp70, c-fos, and c-jun was detectable and increased with further reperfusion. Within the area labelled for these gene products was a central area of less intense labelling which corresponded to the necrotic zone. The immediate early gene product, jun-B, was localized in both the ischaemic and the non-ischaemic area of the hearts. These results suggest that the area of the heart where heat shock and immediate early gene transcripts accumulate, while injured, recovers transcriptional activity, and that the central area where minimal heat shock and immediate early gene transcripts accumulate, does not recover transcriptional activity and is irreversibly injured. [less ▲]

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See detailNutritional and animal husbandry aspects of rearing early life stage of Eurasian perch Perca fluviatilis
Kestemont, Patrick; Mélard, Charles ULg; Fiogbe, Emile et al

in Journal of Applied Ichthyology (1996), 12

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See detailHumanitarian aid and health services in Eastern Kivu, Zaïre: collaboration or competition
Goyens, Philippe; Porignon, Denis ULg; Mugisho Soron'Gane, Etienne et al

in Journal of Refugee Studies (1996), 9(3), 268-280

The population of eastern Kivu, like that of Rwanda, has been surviving in critical conditions for many years. The Rwandan refugees of 1994 fled into an area with its own serious problems. The Zairean ... [more ▼]

The population of eastern Kivu, like that of Rwanda, has been surviving in critical conditions for many years. The Rwandan refugees of 1994 fled into an area with its own serious problems. The Zairean health services in the health districts of Rutshuru, Kirotshe and Masisi, in spite of the political and socioeconomic disintegration of the country, were still functioning, and the local hospitals and health centres, although overwhelmed, contributed to a large extent to the disaster response. Prominent among the major problems facing the local health services were their limited adaptability, the inadequate coordination and collaboration offered to them by the humanitarian aid agencies, and the discrimination between the direct and secondary victims of the emergency. The public health consequences of the Rwandan refugee crisis for the Zairean population constitute an integral part of the disaster. [less ▲]

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See detailAn investigation of the effects of daily physical education in kindergarten and elementary schools
Piéron, Maurice ULg; Cloes, Marc ULg; Delfosse, Catherine et al

in European Physical Education Review (1996), 2(2), 116-132

The project used different methods of data collection: tests, questionnaires and systematic observation of pupils and teachers. In developing a multiple viewpoint approach, we attenpted to sketch a ... [more ▼]

The project used different methods of data collection: tests, questionnaires and systematic observation of pupils and teachers. In developing a multiple viewpoint approach, we attenpted to sketch a detached view of the implementation of DPE and of tis results. Goals were as follows: (1) Exploring the possible effects of the programme in various areas of children's life that do not arise directly from their activities during physical education lessons. (2) Identifying children's school-life context, in order to identify factors influencing several effects of DPE. The various viewports considered, the results, and the knowledge of external variables all allowed us to identify a convergent package of indications, thus allowing us to think in terms of causality. Often, the benefits came from the children's involvement in the experimental programme. Improvements of motor performance in primary school age children arise from a maturation process and from practice and learning. Nevertheless, it is particularly hard to identify the relative contributions of these two sources. Tracing pupil's development, fro the starting level at beginning of the school year, appeared to allow isolation of the part played by children's activities whitin the school environement. In all grade levels, comparisons confirmed pilot classes superiority. Performances were significantly better in many tests. However, during the year, it seemed that the development of performance due to maturation masked the potential effects of physical activities. A decline in favourable attitudes was observed in every school at the beginning and the end of the school year. A slide from very favourable attitudes towards simply favourable attitudes was observed. ... [less ▲]

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See detailLa traversée des candiatures universitaires : Une analyse de cas
Beckers, Jacqueline ULg; Boxus, Elise ULg

in Res Academica (1996), 13(1-2), 85-104

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See detailManifestaciones cardiovasculares en el SIDA
Valdes, E. F.; Rostello, E.; Villanueva, H. R. et al

in Prensa Medica Argentina (1996), 83

Cardiovascular complications in acquired immunodeficiency syndrome. AIDS miocardiopathy has been described since 1983. Physiopathologic events leading to AIDS myocardiopathy are largely unknown: viral ... [more ▼]

Cardiovascular complications in acquired immunodeficiency syndrome. AIDS miocardiopathy has been described since 1983. Physiopathologic events leading to AIDS myocardiopathy are largely unknown: viral miocardiopathy, cytokines induced lesions, nutritional status, microvascular and ischemic miocadiopathy have been suspected. We describe a consecutive series of 50 patients (43 males, 7 women) with HIV infection and severe immunodepression (CD4<200/ml) that had clinical, ECG and echocardiographic evaluation. There were 24 drug addicted patients, 17 homosexual patients, and 10 heterosexuals. Some of these patients had the following opportunistic infections: tuberculosis (n=20), Pneumocystis carinii (n=9), Criptocococis (n=9), oropharyngeal candidosis (n=5), toxoplasmosis (n=5), histoplasmosis (n=1) and Kaposi’s sarcoma (n=2). After clinical evaluation 15/50 patients had some kind of cardiovascular complication: 6 patients had congestive heart failure, 5 patients presented with pericardial effusion, one patient has a reversible cardiac arrest, and one patient had pulmonary valvular endocarditis. After Doppler echocardiographic evaluation (according to the American echocardiography association), we found 20 patients with left ventricular diastolic dysfunction, 9 patients with right ventricular diastolic dysfunction and 6 patients with left ventricular systolic dysfunction. In conclusion, our series found left diastolic dysfunction in nearly 40 % of cases and cardiavasular events (pericarditis, heart congestive failure, arrhythmias) in 26% of cases. Cardiovascular complications have prognostic implications in AIDS and shoul be early detected. Further pathological and immunohistochemical studies are needed to clarify the phyiopathological etiologies in AIDS miocardiopathy. [less ▲]

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See detailMise au point relative à l'utilisation de la gonadolibérine en reproduction bovine. 2. Domaines d'application clinique.
Hanzen, Christian ULg; Houtain, Jean Yves; Laurent, Yves

in Médecin Vétérinaire du Québec (1996), 26(2), 47-54

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See detailAnatomie des monticules micritiques du Frasnien belge et contexte eustatique
Boulvain, Frédéric ULg; Herbosch, A.

in Bulletin de la Société Géologique de France (1996), 167(3), 391-398

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See detailDistribution of inorganic carbon and related parameters in surface waters of the English Channel during spring 1994
Frankignoulle, Michel; Elskens, Marc; Biondo, Renzo ULg et al

in Journal of Marine Systems (1996), 7

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See detailOn the structure of liquid tellurium
Bichara, C.; Raty, Jean-Yves ULg; Gaspard, Jean-Pierre ULg

in Journal of Non-Crystalline Solids (1996), 205-207

The atomic structure and the bonding mechanism in liquid tellurium are investigated by a tight binding Monte Carlo technique. The chain structure is preserved but a third covalent bond emerges with an ... [more ▼]

The atomic structure and the bonding mechanism in liquid tellurium are investigated by a tight binding Monte Carlo technique. The chain structure is preserved but a third covalent bond emerges with an intermediate interatomic separation (3.15 Aring). In addition a bond length alternation inside the chain appears in agreement with recent EXAFS experiments and the valence angles are significantly reduced. The electronic structure is studied and particular attention is paid to the modifications of lone pair interactions in the liquid structure [less ▲]

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See detailUna Cabeza Bien Hecha O Una Cabeza Repleta ?
Crahay, Marcel ULg

in Perspectivas (1996), XXVI(1),

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See detailSpontaneous complete remission and recovery of donor haemopoiesis without GVHD after relapse and apparent marrow graft rejection in poor-prognosis myelodysplastic syndrome.
Beguin, Yves ULg; Collignon, Jacques ULg; Laurent, C. et al

in British Journal of Haematology (1996), 94(3), 507-9

We report a patient with poor-prognosis myelodysplastic syndrome (MDS) after successful treatment of lymphoma, who was given an allogeneic BMT, engrafted and achieved complete remission, but later had a ... [more ▼]

We report a patient with poor-prognosis myelodysplastic syndrome (MDS) after successful treatment of lymphoma, who was given an allogeneic BMT, engrafted and achieved complete remission, but later had a relapse of his MDS with complete disappearance of donor haemopoiesis. After two episodes of CMV pneumonia and continued prophylactic use of ganciclovir thereafter, he experienced a spontaneous complete disappearance of all signs of MDS, including myelofibrosis, and a complete return to donor haemopoiesis. This case is the first one to suggest a graft-versus-leukaemia effect (GVL) in MDS patients. It depicts the complex relationship between GVL, graft-versus-host disease (GVHD) and graft rejection. It could also constitute a clinical illustration of the possible antileukaemic effect of CMV infection and its treatment with ganciclovir. [less ▲]

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See detailA novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain
Jacquemin, Patrick; Hwang, Jung-Joo; Martial, Joseph ULg et al

in Journal of Biological Chemistry (1996), 271(36), 21775-85

We describe the molecular cloning of two novel human and murine transcription factors containing the TEA/ATTS DNA binding domain and related to transcriptional enhancer factor-1 (TEF-1). These factors ... [more ▼]

We describe the molecular cloning of two novel human and murine transcription factors containing the TEA/ATTS DNA binding domain and related to transcriptional enhancer factor-1 (TEF-1). These factors bind to the consensus TEA/ATTS cognate binding site exemplified by the GT-IIC and Sph enhansons of the SV40 enhancer but differ in their ability to bind cooperatively to tandemly repeated sites. The human TEFs are differentially expressed in cultured cell lines and the mouse (m)TEFs are differentially expressed in embryonic and extra-embryonic tissues in early post-implantation embryos. Strikingly, at later stages of embryogenesis, mTEF-3 is specifically expressed in skeletal muscle precursors, whereas mTEF-1 is expressed not only in developing skeletal muscle but also in the myocardium. Together with previous data, these results point to important, partially redundant, roles for these TEF proteins in myogenesis and cardiogenesis. In addition, mTEF-1 is strongly coexpressed with mTEF-4 in mitotic neuroblasts, while accentuated mTEF-4 expression is also observed in the gut and the nephrogenic region of the kidney. These observations suggest additional roles for the TEF proteins in central nervous system development and organogenesis. [less ▲]

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See detailWhere is the Algorithmic Support?
Wolper, Pierre ULg

in ACM Computing Surveys (1996), 28(4es), 58

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See detailPratiques d'auto-formation : une enquête en communauté française de Belgique
Denis, Brigitte ULg

in Cahiers d'Etudes du C U E E P (1996), 32-33

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See detailA novel three-enzyme reaction cycle for the synthesis of N-acetyllactosamine with in situ regeneration of uridine 5'-diphosphate glucose and uridine 5'-diphosphate galactose
Zervosen, Astrid ULg; Elling, Lothar

in Journal of the American Chemical Society (1996), 118(8), 1836-1840

A new three-enzyme reaction cycle consisting of sucrose synthase, UDP glucose 4‘-epimerase, and human β-1,4-galactosyltransferase was established for the synthesis of N-acetyllactosamine (LacNAc) with in ... [more ▼]

A new three-enzyme reaction cycle consisting of sucrose synthase, UDP glucose 4‘-epimerase, and human β-1,4-galactosyltransferase was established for the synthesis of N-acetyllactosamine (LacNAc) with in situ regeneration of UDP galactose. We found that UDP glucose 4‘-epimerase is reductively inactivated in the presence of UMP and acceptor substrates of β-1,4-galactosyltransferase. Reactivation of UDP glucose 4‘-epimerase by the transition state analogues dUDP or dTDP 6-deoxy-d-xylo-4-hexulose in combination with the repetitive batch technique enabled us to use the native enzymes for 11 days in this cycle. With 10 U of sucrose synthase, 5 U of UDP glucose 4‘-epimerase, and 1.25 U of β-1,4-galactosyltransferase, 594 mg of LacNAc could be synthesized. N-Acetyllactosamine was also subsequently converted to Neu5Acα2,6Galβl,4GlcNAc with α-2,6-sialyltransferase and CMP-Neu5Ac. [less ▲]

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See detailParticle Production in a Hadron Collider Rapidity Gap: The Higgs Case
Cudell, Jean-René ULg; Hernandez, Oscar F

in Nucl. Phys. (1996), B471

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See detailVariations autour de la définition des fonctions convexes
Bair, Jacques ULg; Haesbroeck, Gentiane ULg

in Mathématique et Pédagogie (1996), 105

Dans le cadre des fonctions réelles, le concept de convexité est présenté dans divers registres sémiotiques, analytiques ou graphiques.

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See detailAcute bronchial obstruction following inhalation of PAF in asthmatic and normal subjects: comparison with methacholine.
Louis, Renaud ULg; Radermecker, Maurice ULg

in European Respiratory Journal (1996), 9(7), 1414-20

Platelet-activating factor (PAF) may play a role in the pathophysiology of asthma but controversies exist about bronchial responsiveness toward this mediator in asthma. We have compared the variations in ... [more ▼]

Platelet-activating factor (PAF) may play a role in the pathophysiology of asthma but controversies exist about bronchial responsiveness toward this mediator in asthma. We have compared the variations in the specific conductance (sGaw) and forced expiratory volume in one second (FEV1) in 12 asthmatics and 12 normal subjects after inhalation of doubling doses of PAF (15-120 micrograms) and methacholine (18 to at least 144 micrograms). In order to take into account a possible tachyphylaxis, we compared PAF dose-response curves performed on one day with the curves obtained by giving the same doses separately on different days. Repeated inhalations of doubling doses of PAF caused sGaw and FEV1 to plateau after the second dose in each group, whereas methacholine provoked a dose-related decrease in sGaw and FEV1. A dose-dependent decrease in the functional indices was restored when the different doses of PAF were administered on separate days. In both groups, the fall in sGaw after inhalation of 60 micrograms as a single dose was higher than that achieved when this dose was given during a full bronchial challenge. The falls in sGaw and FEV1 after PAF inhalation were significantly higher in the asthmatics than in the normal subjects. The provocative dose of PAF causing a 35% fall in sGaw (PD35,sGaw) PAF was only twofold lower in the asthmatics than in the normal subjects (p < 0.05), while it was 11 fold lower for methacholine (p < 0.001). When the PD35,sGaw values were compared, PAF was found on a molar basis to be 33 fold more potent than methacholine in the normal subjects, but only fivefold more potent in the asthmatics (p < 0.05). The percentage falls in FEV1 (calculated by interpolation) for a 35% fall in sGaw, were greater in asthmatics than in normals both for methacholine (p < 0.05) and PAF (p = 0.09). Our results demonstrate a tachyphylaxis after inhalation of platelet-activating factor in normal subjects and asthmatics, and show that asthmatics develop a greater bronchial obstruction than normal subjects even if methacholine is more sensitive than platelet-activating factor at discriminating between the two groups. [less ▲]

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See detailGlycoprotein H (gII/gp108) and glycoprotein L form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1.
van Drunen Littel-van den Hurk, S.; Khattar, S.; Tikoo, S. K. et al

in Journal of General Virology (The) (1996), 77 ( Pt 7)

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by two ... [more ▼]

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by two different panels of monoclonal antibodies. The gII- and gp 108-specific monoclonal antibodies were shown to react with the same protein, which was identified by N-terminal sequencing as the homologue of herpes simplex virus type 1 (HSV-1) gH. When BHV-1 gH was purified by immunoadsorbent chromatography, gL was co-purified. The gH-gL complex induced the production of antibodies that neutralized virus infectivity and inhibited virus penetration. Affinity-purified gH-gL did prevent penetration, but not attachment of BHV-1, which suggests that the gH-gL complex is essential for penetration of BHV-1 into susceptible cells. [less ▲]

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See detailMise au point relative à l'utilisation de la gonadolibérine en reproduction bovine. 1. Justifications physiologiques de son application au traitement de l'infertilité
Hanzen, Christian ULg; Houtain, Jean Yves; Laurent, Yves

in Médecin Vétérinaire du Québec (1996), 26(1), 7-10

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See detailCloning and expression of a new HOXC6 transcript encoding a repressing protein
Chariot, Alain ULg; Castronovo, Vincenzo ULg; Le, P. et al

in Biochemical Journal (1996), 319

Homeodomain-containing proteins are transcription factors that regulate the co-ordinated expression of multiple genes involved in development, differentiation and malignant transformation. In an attempt ... [more ▼]

Homeodomain-containing proteins are transcription factors that regulate the co-ordinated expression of multiple genes involved in development, differentiation and malignant transformation. In an attempt to characterize expressed homeobox (HOX) genes in breast cancer cells, we cloned two distinct HOXC6 transcripts from an MCF7 cDNA library, Interestingly, one of them represents a new HOXC6 mRNA encoding a homeodomain-containing protein harbouring a unique N-terminal sequence. Moreover we demonstrate that this HOXC6 transcript is less abundant in human breast cancer cells than in non-tumorigenic cell lines, is detected in breast carcinomas and adjacent tissues and is expressed in a variety of human tumours. In addition, transient co-transfection experiments illustrated that both HOXC6 transcripts encode gene products that repress transcription from a HOX binding sequence in MDA-MB231 cells and co-operate with other HOX gene products such as HOXB7 on their target genes. Taken together, our results suggest that HOXC6 proteins might contribute to the breast cell phenotype through co-operative interactions with other HOX-derived proteins and repression of their target genes. [less ▲]

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See detailModelling of multiphase flow in packed beds by computer-assisted x-ray tomography
Toye, Dominique ULg; Marchot, Pierre ULg; Crine, Michel ULg et al

in Measurement Science & Technology (1996), 7(3), 436-443

The high resolution which can be obtained by computer-assisted x-ray tomography is used to investigate Various scale heterogeneities of the flow texture characterizing multiphase flow in packed beds. The ... [more ▼]

The high resolution which can be obtained by computer-assisted x-ray tomography is used to investigate Various scale heterogeneities of the flow texture characterizing multiphase flow in packed beds. The direct counting of 'irrigated' pixels allows the determination of the number of individual liquid rivulets as well as their size. The evolution of the liquid flow texture versus the liquid velocity agrees quite well with the predictions of a percolation-based model. This agreement is further confirmed by the comparison of the experimental hold-up values averaged over the column cross-section with the theoretical model. [less ▲]

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See detailCombined enzymatic synthesis of nucleotide (deoxy) sugars from sucrose and nucleoside monophosphates
Zervosen, Astrid ULg; Stein, Andreas; Adrian, Holger et al

in Tetrahedron (1996), 52(7), 2395-2404

Abstract: The synthesis of NDP-glucose 3a-d (N= A, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside diphosphates 2a-d from their corresponding nucleoside ... [more ▼]

Abstract: The synthesis of NDP-glucose 3a-d (N= A, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside diphosphates 2a-d from their corresponding nucleoside monophosphates la-d by different kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-alpha-D-xylo-4-hexulose 5 from ld on a preparative scale. By using the repetitive batch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a-d, 5 could be realized on a 0.1 - 0.5 g scale. [less ▲]

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See detailEffets toxiques de l’ozone atmosphérique sur le système respiratoire. Brève revue des connaissances actuelles
Delaunois, A.; Gustin, Pascal ULg; Nemmar, R. et al

in Annales de Médecine Vétérinaire (1996), 140

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See detailLa titanite de Bastogne (Belgique) et les minéraux associés
Hatert, Frédéric ULg; Fransolet, André-Mathieu ULg; Houssa, Michel

in Bulletin de la Société Royale des Sciences de Liège (1996), 65(6), 387-397

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See detailCes histoires que l'on construit et que l'on se raconte...
Guillaume, Jean-François ULg

in Cahiers Internationaux de Sociologie (1996), 100

A partir des réflexions de Paul Ricoeur sur la temporalité et la narrativité, l'A. envisage les processus discursifs de construction identitaire chez les jeunes gens en fin de scolarité obligatoire. L ... [more ▼]

A partir des réflexions de Paul Ricoeur sur la temporalité et la narrativité, l'A. envisage les processus discursifs de construction identitaire chez les jeunes gens en fin de scolarité obligatoire. L'identité en construction est appréhendée à travers le récit qu'ils donnent de leurs projets d'avenir et de leur histoire familiale. La présentation de soi, perçue dans cette double dimension, est soumise à un ensemble de schèmes ou de modèles narratifs culturellement ancrés et socialement véhiculés. Elle prend forme et se constitue en une totalité cohérente et intelligible par une « mise en intrigue » de la diversité des péripéties vécues ou imaginées. [less ▲]

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See detailOn the residual advection of passive constituents
Delhez, Eric ULg

in Journal of Marine Systems (1996), 8(3-4), 147-169

In this paper, the different approaches used to simulate the long-term advection of passive constituents on tidal shelves are discussed in the framework of large scale hydrodynamic modelling. The direct ... [more ▼]

In this paper, the different approaches used to simulate the long-term advection of passive constituents on tidal shelves are discussed in the framework of large scale hydrodynamic modelling. The direct approach in which the unsteady mesoscale (tides and storm surges) and macroscale (monthly or seasonal mean processes) currents are used is very demanding in computer resources (CPU and memory). On the other hand, the use of residual velocities gives a larger understanding of the long-term transport processes and simplifies numerical treatments. However, the definition of such appropriate velocities is still an open question. In the context of large scale models, Lagrangian residuals are not applicable. Eulerian residual transport velocities fail to represent long-term motions when tidal non-linearities are important. The first order Lagrangian residual velocity introduced by Feng et al. (1986a) and generalized here is shown to be a very good solution. The North-Western European Continental Shelf (NWECS) is used as an example to compare the numerical solutions obtained with the different approaches. [less ▲]

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See detailEfficacy of nonsteroidal antiinflammatory drugs in postoperative pain
Joris, Jean ULg

in Acta Anaesthesiologica Belgica (1996), 47(3), 115-123

Historically, NSAIDs, used for more than 25 years to treat rheumatologic diseases, were then introduced to relieve pain after tooth extraction, and finally about 10 years ago for postoperative analgesia ... [more ▼]

Historically, NSAIDs, used for more than 25 years to treat rheumatologic diseases, were then introduced to relieve pain after tooth extraction, and finally about 10 years ago for postoperative analgesia. NSAIDs have been increasingly used over the past ten years in the treatment of postoperative pain, such that they now play an important role in the management of postoperative analgesia, either alone or combined with opioids. When used alone, they are effective in relieving minor or moderate pain such as that seen after maxillofacial, minor orthopedic, or some ambulatory surgical procedures, and postpartum pain (episiotomy). In these indications, the main benefit as compared with opioids is the lack of respiratory depression, nausea and vomiting. Since these side effects delay discharge from the hospital after ambulatory surgery, the use of NSAIDs may result in faster recovery and earlier discharge. Because of the ceiling effect of NSAIDs, their efficacy as sole agents is usually insufficient to treat pain after major surgery (orthopedic, abdominal, thoracic). NSAIDs should then be combined with opioids. As part of a balanced analgesia regimen, NSAIDs will allow for opioid-sparing, and might subsequently reduce opioid-mediated side effects. A 20 to 50% reduction in opioid consumption, sometimes with improved quality of analgesia, has been reported using different NSAIDs following various types of surgery. Better respiratory function, improved sleep quality, and faster recovery of gastrointestinal function have been reported with NSAIDs. However, the use of NSAIDs has not been shown to be associated with improved outcome or more rapid recovery. [less ▲]

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See detailThe frequency of cutaneous vasculitis is not increased in patients with rheumatoid arthritis treated with methotrexate
Kaye, O.; Beckers, C. C.; Paquet, P. et al

in Journal of Rheumatology (1996), 23(2), 253-257

OBJECTIVE: To analyse the frequency, type, and immunohistological features of clinical cutaneous vasculitis developing in patients with rheumatoid arthritis (RA) treated or not with methotrexate as well ... [more ▼]

OBJECTIVE: To analyse the frequency, type, and immunohistological features of clinical cutaneous vasculitis developing in patients with rheumatoid arthritis (RA) treated or not with methotrexate as well as their demographic, clinical and biological characteristics. METHODS: Ninety-one patients with RA receiving 9.5 mg/wk of methotrexate (MTX) were compared for an average observation time of 18 months to 130 matched patients with RA treated with various drugs excluding MTX. RESULTS: Whether receiving MTX or not, 5.4% of patients with RA developed a clinical cutaneous vasculitis. There were significant differences between both groups for 2 variables only: a higher percentage of polyneuropathies and a higher level of immune complex-plasma levels in non-MTX patients. The immunohistological analysis did not differentiate both groups. CONCLUSION: The percentage of cutaneous vasculitis that developed under MTX therapy was not different from that occurring as a natural complication of longstanding severe RA. [less ▲]

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See detailQuantitative Densitometric Evaluation of Harpagoside in the Secondary Roots of Harpagophytum procumbens D.C.
Poukens-Renwart, Pascale; Tits, Monique ULg; Angenot, Luc ULg

in Journal of Planar Chromatography- Modern TLC (1996), 9(May/June), 199-202

Harpagophytum procumbens D.C. (Devil's Claw) secondary roots are used in Europe as a home remedy for lack of appetite, dyspeptic complaints, and in supportive therapy for degenerative disorders of the ... [more ▼]

Harpagophytum procumbens D.C. (Devil's Claw) secondary roots are used in Europe as a home remedy for lack of appetite, dyspeptic complaints, and in supportive therapy for degenerative disorders of the locomotor system. The main characteristic iridoid glucoside of the plant is harapagoside. An HPTLC method has been developed for the quantitative determination of harpagoside in the secondary roots and extracts of this plant. The mobile phase used is an optimized version of one described in the literature.It furnished the most homogenous spots for quantitative densitometric evaluation ( at lambda= 286 nm, the wavelenght of maximum absorption for harapgoside).The results have been compared with those obtained afetr visualization with sulfuric acid-vanillin reagent and those obtained using a previously published HPLC method; To check that separation was complete, the spectra of harpagoside obtained from Devil's Claw extracts before and after visualization were superimposed on thos of harpagoside standard. [less ▲]

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See detailLes infections utérines dans l’espèce bovine : 2. Thérapeutiques anti-infectieuses et hormonales
Hanzen, Christian ULg; Houtain, J. Y.; Laurent, Y.

in Point Vétérinaire (1996), 28(numéro spécial), 1019-1025

The choice of an antibiotic or antiseptic treatment must take into account, the administrative route, the individual factors (nature of symptoms, stage postpartum, stage in the cycle) and the agents used ... [more ▼]

The choice of an antibiotic or antiseptic treatment must take into account, the administrative route, the individual factors (nature of symptoms, stage postpartum, stage in the cycle) and the agents used (nature of germs, minimal inhibiting concentration, pharmacological properties, milk residue). Hormonal treatment is an interesting alternative, which must be considered on an individual basis rather than systematically at herd level. For prevention, it should only be used on animals presenting a high risk factor of infection [less ▲]

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See detailMagnetic alignment in 2212 Bi-based superconducting system .1. Magnetic orientation of Bi2Sr2Ca1-x(RE)(x)Cu2O8-y [(RE)=Gd, Dy, Ho, Er] powder dispersed in epoxy resin at room temperature
Stassen, S.; Cloots, Rudi ULg; Vanderbemden, Philippe ULg et al

in Journal of Materials Research (1996), 11(5), 1082-1085

The magnetic anisotropy of rare-earth substituted 2212 materials (Bi(2)Sr(2)Ca(0.8)RE(0.2)Cu(2)O(x) with RE = Gd, Dy, Ho, Er) is put into evidence. Superconducting powder dispersed in epoxy resin is ... [more ▼]

The magnetic anisotropy of rare-earth substituted 2212 materials (Bi(2)Sr(2)Ca(0.8)RE(0.2)Cu(2)O(x) with RE = Gd, Dy, Ho, Er) is put into evidence. Superconducting powder dispersed in epoxy resin is oriented under an external magnetic field (4 T) in a direction that depends on the nature of the rare-earth used in the substitution. Both directions of observation (parallel or perpendicular to the field) were investigated. Splitting of (00l) peaks is neatly observed and discussed. [less ▲]

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See detailChromosomal location of fifteen unique mouse KRAB-containing zinc finger loci
Marine, J. C.; Gilbert, D. J.; Bellefroid, Eric J. et al

in Mammalian Genome : Official Journal of the International Mammalian Genome Society (1996), 7(6), 413-6

The mammalian genome contains hundreds if not thousands of zinc finger protein (Zfp) genes. While the function of most of these genes remains to be determined, it is clear that a few of them play ... [more ▼]

The mammalian genome contains hundreds if not thousands of zinc finger protein (Zfp) genes. While the function of most of these genes remains to be determined, it is clear that a few of them play important roles in gene regulation and development. In studies described here, we have used an interspecific mouse backcross mapping panel to determine the chromosomal location of 15 KRAB-containing zinc finger loci. These loci map to nine different mouse autosomes and the X Chromosome (Chr). Two Chrs, 7 and 9, contain cosegregating pairs of KRAB-containing Zfp genes, indicating that the KRAB-containing Zfp genes have evolved through processes involving regional as well as genome-wide duplication events. [less ▲]

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See detailDensitometric Evaluation of Gentiopicroside in the Roots of Gentiana lutea L.
Bodart, Patricia; Poukens-Renwart, Pascale; Wauters, Jean-Noël ULg et al

in Journal of Planar Chromatography- Modern TLC (1996), 9(March/April), 143-145

As no satisfactory method existed for the separation of gentiopicroside from other components of gentian roots, we have decided to undertake some experiments.In this communication, we report the use of a ... [more ▼]

As no satisfactory method existed for the separation of gentiopicroside from other components of gentian roots, we have decided to undertake some experiments.In this communication, we report the use of a novel mobile phase and a different detection system. Our HPTLC method is a fast and reliable procedure which may be used for quantification of gentiopicroside in roots and extracts of Gentiana lutea, whether for routine analysis or for studying stability. The TLC method is also compared with an HPLC method. [less ▲]

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See detailBrain hypometabolism of glucose in anorexia nervosa: Normalization after weight gain
Delvenne, Véronique; Goldman, Serge; De Maertelaer, Viviane et al

in Biological Psychiatry (1996), 40(8), 761-768

Using positron emission tomography and (18-F)-fluorodeoxyglucose, we studied cerebral glucose metabolism in 10 anorectic girls within their underweight state and after weight gain. Ten age- and sex ... [more ▼]

Using positron emission tomography and (18-F)-fluorodeoxyglucose, we studied cerebral glucose metabolism in 10 anorectic girls within their underweight state and after weight gain. Ten age- and sex-matched healthy volunteers were used as controls. Both groups were scanned during rest, eyes closed and with low ambient noise. In absolute values, the underweight anorectic patients, when compared to control subjects, showed a global (p = 0.002) and regional (p ≤ 0.001) hypometabolism of glucose which normalized with weight gain. In relative values, no global difference could be assessed between underweight anorectic patients and controls but a trend can, nevertheless, be observed toward parietal and superior frontal cortex hypometabolism associated with a relative hypermetabolism in the caudate nuclei and in the inferior frontal cortex. After weight gain, all regions normalized for absolute and relative values, although a trend appears toward relative parietal hypometabolism and inferior frontal cortex hypermetabolism in weight gain anorectic patients. Absolute brain glucose hypometabolism might result from neuroendocrinological or morphological aspects of anorexia nervosa or might be the expression of altered neurotransmission following deficient nutritional state. As some differences exists in relative values in underweight patients and tend to persist in weight gain states, this could support a potential abnormal cerebral functioning, a different reaction to starvation within several regions of the brain or different restoration rates according to the region. [less ▲]

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See detailAugustus Latinus. Purisme, pouvoir, politique et idéologie sous Auguste
Rochette, Bruno ULg

in Bulletin de l'Institut Historique Belge de Rome (1996), 66

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See detailMembrane-type matrix metalloproteinase-1 expression at the site of human placentation
Nawrocki, B.; Polette, M. E.; Marchand, V. et al

in Placenta (1996), 17(8), 565-72

Human trophoblast implantation is a highly regulated process of invasion that requires action of proteolytic enzymes to degrade extracellular matrix components of the endometrium. Among these enzymes ... [more ▼]

Human trophoblast implantation is a highly regulated process of invasion that requires action of proteolytic enzymes to degrade extracellular matrix components of the endometrium. Among these enzymes, matrix metalloproteinases (MMPs) seem to be particularly important in this degradative process. We previously showed that gelatinase A is extensively expressed in vivo in the human placenta. A new MMP, MT-MMP-1 (membrane-type matrix metalloproteinase-1), which is thought to activate progelatinase A, has recently been described. In this study, we examined the expression of MT-MMP-1, by immunohistochemistry and in situ hybridization, in human placental bed biopsies taken during the first trimester of gestation. Human first trimester intermediate trophoblasts synthesized MT-MMP-1 mRNAs and the protein. The MT-MMP-1 pattern of distribution in placental beds was similar to that of gelatinase A, suggesting a pivotal role for MT-MMP-1 in placentation, perhaps by activating progelatinase A. [less ▲]

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See detailDes pèlerins latins en Terre Sainte. Rencontre de langues et de cultures
Rochette, Bruno ULg

in Byzantion : Revue Internationale des Etudes Byzantines (1996), 66

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See detailField evaluation of pregnancy diagnosis using bovine pregnancy-associated glycoprotein (bPAG)
Skinner, J. G.; Gray, D.; Gebbie, F. E. et al

in Cattle Practice (1996), 4(3), 281-284

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See detailMolecular Evidence That The Whitefly-Transmitted Sweetpotato Mild Mottle Virus Belongs To A Distinct Genus Of The Potyviridae
Colinet, Dominique; Kummert, Jean; Lepoivre, Philippe ULg

in Archives of Virology (1996), 141(1), 125-135

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See detailLes champs électromagnétiques de faible intensité produisent une vague calcique dans les fibroblastes
Bomans, J.; Lambert, C. A.; Scarpa, B. et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1996), 151(3-4), 243-9250-2

Human fibroblasts display a Ca2+ wave after irradiation with an electromagnetic field (EMF) of low intensity (100 to 900 microT) as seen by LASER confocal microscopy and excitation of Fluo 3. The number ... [more ▼]

Human fibroblasts display a Ca2+ wave after irradiation with an electromagnetic field (EMF) of low intensity (100 to 900 microT) as seen by LASER confocal microscopy and excitation of Fluo 3. The number of excited cells is proportional to the intensity of EMF between 100 and 900 microT. Cellular activation by a dialysable serum factor is required to induce the Ca2+ wave. It also depends on extracellular Ca2+ and active tyrosine kinases and phospholipase C gamma. [less ▲]

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See detailLe Calendrier et son Histoire
Biémont, Emile ULg

in Bulletin de la Classe des Sciences. Académie Royale de Belgique (1996), 6e série(VII), 15-71

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See detailLes reliques sur ordinateur
George, Philippe ULg

in Le médiéviste et l'ordinateur (1996)

Detailed reference viewed: 6 (1 ULg)