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See detailA concise guide to the temporal maze
Richelle, Marc ULg

in Psychologica Belgica (1993), 33(2), 135-145

The paper provides a concise historical survey of the expanding field of time psychology, pointing to the important contributions, in its early stages as well as today, of European psychologists. An ... [more ▼]

The paper provides a concise historical survey of the expanding field of time psychology, pointing to the important contributions, in its early stages as well as today, of European psychologists. An evaluation of past and current research is proposed, and prospects for further development suggested, especially with regard to neglected areas and to issues related to applied situations. [less ▲]

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See detailMesures quantitatives de l'erythropoiese. Le recepteur soluble de la transferrine.
Beguin, Yves ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1993), 148(1-2), 100-4105-7

Iron transport in plasma is carried out by transferrin, which donates iron to cells through its interaction with a specific membrane receptor. The soluble form of the transferrin receptor has been ... [more ▼]

Iron transport in plasma is carried out by transferrin, which donates iron to cells through its interaction with a specific membrane receptor. The soluble form of the transferrin receptor has been identified in plasma. Dosage of the soluble receptor represents a simple, reliable, quantitative assay of erythropoiesis, which should replace the standard ferrokinetic measurement. Using this dosage, it is now possible to investigate the pathophysiology of anemia in many conditions, and to analyze the erythropoietic response to treatment with recombinant erythropoietin. [less ▲]

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See detailLocalisation scintigraphique de tumeurs neuroendocrines et de leurs métastases par un analogue de la somatostatine: [111In-DTPA-d-phe1] octréotide.
Beckers, Albert ULg; Pham, V. T.; Abs, R. et al

in Revue Médicale de Liège (1993), 48(6), 326-34

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See detailOkadaic acid, a protein phosphatase inhibitor, enhances transcription of a receptor gene containing sequence A of the human prolactin promoter
Wera, S.; Belayew, A.; Martial, Joseph ULg

in Molecular Endocrinology (1993), 7(8), 965-71

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter ... [more ▼]

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter. We have investigated whether protein phosphatases could be involved in this regulation. GC-type rat pituitary tumor cells were transfected with sequence -138 to -35 of the hPRL gene promoter, upstream from a thymidine kinase promoter and a chloramphenicol acetyltransferase (CAT) reporter gene. Addition of okadaic acid (OA), a specific inhibitor of protein phosphatases 1 and 2A, stimulates transient expression of the CAT gene. The dose-response curve shows a maximal effect at 25 nM OA (2.2-fold stimulation above controls). The OA effect is also observed with a natural 4500-base pair hPRL promoter. A single copy of the hPRL promoter sequence -115 to -85 (sequence A) confers to a thymidine kinase-CAT construct an identical response to OA, whereas a single copy of the proximal Pit-1 binding site does not. Synergism is observed between cAMP and OA in activating PRL gene transcription. This synergism is also observed with a single copy of sequence A. The effect of cAMP is not mediated by an L-type Ca2+ channel, since addition of the Ca2+ channel antagonist verapamil does not decrease it, nor does complexing extracellular Ca2+ significantly reduce it. Furthermore, OA and the Ca2+ channel opener BAY K8644 exert additive effects. [less ▲]

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See detailSynthetic model peptides for apolipoproteins. II. Characterization of the discoidal complexes generated between phospholipids and synthetic model peptides for apolipoproteins.
Corijn, J.; Deleys, R.; Labeur, C. et al

in Biochimica et Biophysica Acta (1993), 1170(1), 8-16

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied ... [more ▼]

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied. The sequences of the peptides were derived from that of the 18A peptide and designed to either enhance or decrease ionic interactions between pairs of peptides, as described in the accompanying paper. Complexes were prepared with dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), or with DPPC and cholesterol, and isolated on a Superose 6HR column. Association kinetics for the DMPC-peptides complexes were followed by measuring the turbidity as a function of the temperature. The diameters of the DPPC-peptide complexes, measured by gradient gel electrophoresis (GGE), were about 120 A. Fluorescence polarization measurements after labeling with diphenyl hexatriene (DPH) yielded transition temperatures of, respectively, 40.6, 41.5 and 41.8 degrees C for the DPPC/18AM1-, DPPC/18AM4- and DPPC/18A-peptide complexes. These values were confirmed by differential scanning calorimetry. Circular dichroism and infrared spectroscopy revealed that the peptides adopt an alpha-helical structure in solution and this percentage increased from 30-40% in the free peptides up to 50-60% in the complexes. Attenuated total reflection (ATR) infrared measurements of the complexes indicated that the peptides are oriented parallel to the acyl chains of the phospholipid bilayer. Denaturation of the peptides and of the peptide-lipid complexes was monitored by Trp fluorescence under addition of increasing amounts of GdmCl. The mid-points of the denaturation curves lie at, respectively, 0.05, 0.25 and 0.35 M GdmCl for the 18AM4, 18A and 18AM1 peptide and are shifted towards higher GdmCl concentrations after peptide-lipid binding. GdmCl denaturation decreased the alpha-helical content of the peptides and of the complexes, as monitored by circular dichroism measurement. The helix to random coil structure transition occurred at, respectively, 2.1, 2.2, and 2.0 M GdmCl for 18A, 18AM1 and 18AM4, compared to 5.1, 5.0, and 5.3 M in the corresponding complexes. These data suggest altogether that the structural properties, the mode of lipid-protein association and the stability of the phospholipid-peptide complexes are similar to those of native plasma apolipoproteins. The 18A and 18AM4 peptides which contain charged residues along the edge of the helix, leading to salt bridge formation between peptides were shown to mimic the amphipathic helices of the plasma apolipoproteins. [less ▲]

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See detailPolarization-corrected electrostatic potentials of aromatic compounds
Dehareng, Dominique ULg; Dive, Georges ULg; Ghuysen, Jean-Marie ULg

in Journal of the American Chemical Society (1993), 115(15), 6877-6882

The electrostatic potentials (EPS) corrected for polarization (TPS) of the aromatic compounds benzene, aniline, chlorobenzene, nitrobenzene, phenol, benzamide, and N-phenylacetamide have been calculated ... [more ▼]

The electrostatic potentials (EPS) corrected for polarization (TPS) of the aromatic compounds benzene, aniline, chlorobenzene, nitrobenzene, phenol, benzamide, and N-phenylacetamide have been calculated at the ab initio SCF level within three basis sets: 6-31G**, MINI-1, and STO-3G. For chlorobenzene in its MINI-1-optimized geometry, the calculation was also performed within MINI-1**. By reference to 6-31G**, the MINI-1-computed EP is much more satisfactory than the STO-3G-computed EP, whereas the MINI-I and STO-3G basis sets give very similar total potentials corrected for polarization (TPs). The MINI-1** basis set appears to be miscalibrated for computing EPs. It provides qualitative results that differ from those obtained with the 6-31G** basis set. The EP has a negative well above the middle of the benzene ring, while the TP exhibits a negative crown just above the benzene carbon atoms, where electrophilic attack takes place. The TP calculated for the interaction of nitrobenzene with a hydride ion instead of a proton allowed analyzation of the effects of polarization on the positive EP above the N-C bond. [less ▲]

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See detailEnhancement of metastatic potential of murine and human melanoma cells by laminin receptor peptide G: attachment of cancer cells to subendothelial matrix as a pathway for hematogenous metastasis.
Taraboletti, G.; Belotti, D.; Giavazzi, R. et al

in Journal of the National Cancer Institute (1993), 85(3), 235-40

BACKGROUND: Stable anchorage of circulating cancer cells to the vasculature is a critical step in the formation of hematogenous metastases. Although the basement membrane glycoprotein laminin clearly ... [more ▼]

BACKGROUND: Stable anchorage of circulating cancer cells to the vasculature is a critical step in the formation of hematogenous metastases. Although the basement membrane glycoprotein laminin clearly plays a crucial role in this event, the exact interactive pathways among cancer cells, laminin, and the vessel wall have not been elucidated. In a previous study, we identified synthetic peptide G, which contains the laminin-binding domain of the 67-kd laminin receptor and which inhibits tumor cell adhesion to endothelial cells. PURPOSE: To assess the role of the interaction between laminin and the 67-kd laminin receptor in hematogenous metastasis formation, we studied the effect of peptide G on melanoma cell behavior in vivo and in vitro. METHODS: The effect of peptide G and control peptides was studied in vivo on lung retention and colonizing potential of murine (B16BL6) and human (A2058) melanoma cells injected intravenously in C57BL/6 and nude mice, respectively. In addition, their effect on cell adhesion and chemotaxis to laminin and on binding of iodine 125-labeled laminin to cells was studied in vitro. RESULTS: In vivo, pretreatment of cells with peptide G resulted in a two- to 10-fold significant increase in the number of experimental lung metastases. A significant relative increase in lung retention of peptide G-treated tumor cells was observed 48 hours after injection, although after 4 hours a partial reduction was observed. In vitro, peptide G significantly increased laminin binding and cancer cell adhesion to laminin and subendothelial matrix, whereas chemotaxis to laminin was significantly inhibited. CONCLUSIONS: Peptide G differentially affected the biological response of cancer cells to laminin. In vitro, it increased laminin binding and cell adhesion to laminin and subendothelial matrix, whereas it inhibited cell chemotaxis to laminin. In vivo, the overall effect of peptide G was an augmentation of lung metastasis. IMPLICATIONS: Our findings suggest that direct adhesion of tumor cells to the subendothelial matrix is a main pathway for hematogenous metastases and that tumor cell-matrix interaction may be more relevant than tumor cell-endothelial cell attachment in this process. [less ▲]

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See detailSYNCYTIOTROPHOBLASTIC LOCALIZATION OF THE HUMAN GROWTH-HORMONE VARIANT MESSENGER-RNA IN THE PLACENTA
Scippo, Marie-Louise ULg; Frankenne, Francis ULg; HOOGHEPETERS, ELisabeth et al

in Molecular & Cellular Endocrinology (1993), 92(2), 7-13

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental ... [more ▼]

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using P-32-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only. [less ▲]

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See detailA method of comparison of two close batches data: Application to analysis of fog formation causes
Boreux, Jean-Jacques ULg; Guiot, Joël

in Geophysical Research Letters (1993), 20(12), 1179-1182

Given suitable conditions of air temperature and humidity, the density of a fog and its microphysical properties depend mainly on the availability of cloud condensation nuclei (CCN) and their nature. Fogs ... [more ▼]

Given suitable conditions of air temperature and humidity, the density of a fog and its microphysical properties depend mainly on the availability of cloud condensation nuclei (CCN) and their nature. Fogs become particularly dense near certain industrial plants because of high concentration of hygroscopic combustion particles in the air. Their role in dense fog formation is estimated by comparing the local climates and CCN concentrations at two similar sites, the first being more subject to air pollution and dense fogs than the second. Orthogonal regression is applied to three meteorological variables (air temperature, relative humidity, wind speed) and CCN concentration. As we compare very close variables, bootstrap provides precise confidence intervals independent of Gaussian assumptions. Two sites are compared: they are located in the Meuse valley (Belgium) at a distance of about 15 km. We found that the local climate of the polluted site is not only colder and wetter but also richer in CCN that the control site. These results suggest interactions of natural and anthropogenic causes in dense fog formation at industrial site. This method is useful in various domains of geophysics when correlated time series have to be compared. [less ▲]

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See detailAnalyse morphométrique des poudres : Une approche systématique et robuste par la Morphologie Mathématique
Pirard, Eric ULg

in Revue de Métallurgie - Sciences et Génie des Matériaux (1993), 9

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See detailNeutrophilic Eccrine Hidradenitis. A Case Report
Nikkels, Arjen ULg; Hansen, Isabelle ULg; Collignon, Jacques ULg et al

in Acta Clinica Belgica (1993), 48(6), 397-400

Neutrophilic eccrine hidradenitis (NEH) is a rare neutrophilic dermatosis occurring most frequently during induction chemotherapy for a variety of malignancies. We report a case of NEH in a 41-year-old ... [more ▼]

Neutrophilic eccrine hidradenitis (NEH) is a rare neutrophilic dermatosis occurring most frequently during induction chemotherapy for a variety of malignancies. We report a case of NEH in a 41-year-old woman with acute myeloblastic leukemia under daunorubicin, cytarabine and etoposide chemotherapy. She developed red, tender and painful nodules on a shoulder. The lesions resolved spontaneously. Histological examination is mandatory as the clinical presentation of this dermatosis is highly polymorphic. Leukemia cutis, sepsis, deep fungal infection and Sweet's syndrome must be excluded as these implicate different therapies. [less ▲]

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See detailVers une optimalisation de la formation langagière de futurs cadres: une expérience en semi-autonomie à la K.U.Leuven
Sanctobin, V.; Slootmaekers, A.; Vanden Berghe, Kristine ULg et al

in Association Belge de Linguistique Appliquée Papers (1993), 15

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See detailInteraction between the 67 kilodalton metastasis-associated laminin receptor and laminin.
Cioce, V.; Margulies, I. M.; Sobel, M. E. et al

in Kidney International (1993), 43(1), 30-7

Normal and neoplastic cells interact with laminin via a variety of cell surface proteins. The specific binding sites on laminin for each particular cell surface laminin-binding protein have not yet been ... [more ▼]

Normal and neoplastic cells interact with laminin via a variety of cell surface proteins. The specific binding sites on laminin for each particular cell surface laminin-binding protein have not yet been identified. In this study, the interaction between laminin and the high affinity metastasis-associated 67 kD laminin receptor (67 LR) was investigated by electron microscopy using the rotary shadowing technique. Laminin receptor that was purified from human colon carcinoma metastases appeared as a globular structure with a diameter of 5.2 +/- 0.8 nm. The 67 LR specifically bound to laminin on its long arm close to the intersection of the long and the short arms. There was no specific interaction of bovine serum albumin with laminin. Biochemical confirmation of the rotary shadowing experiments included slot blot solid phase assays in which [I125]-labeled 67 LR bound in a dose dependent manner to laminin as well as to the chymotrypsin resistant (C1) fragment of laminin that contains a short piece of the long arm. [I125]-labeled 67 LR did not bind to the pepsin resistant (P1) fragment of laminin that did not contain that segment on the long arm. This study therefore identifies the binding site on laminin for the 67 kD metastasis-associated laminin receptor as a region on the long arm of laminin close to the intersection of the four arms. [less ▲]

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See detailMonoclonal-Antibodies To Bovine Growth-Hormone Potentiate Hormonal Activity In-Vivo By Enhancing Growth-Hormone Binding To Hepatic Somatogenic Receptors
Massart, Serge; Maiter, D.; Portetelle, Daniel ULg et al

in Journal of Endocrinology (1993), 139(3),

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See detailComparative study of the body surface electrocardiogram in double-muscled and conventional calves.
Amory, Hélène ULg; Rollin, Frédéric ULg; Genicot, Bruno et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1993), 57(3), 139-45

The purpose of this work was to study electrocardiographic features of double-muscled beef cattle. Electrocardiograms were recorded on one to six occasions from each of a group of 18 conventional calves ... [more ▼]

The purpose of this work was to study electrocardiographic features of double-muscled beef cattle. Electrocardiograms were recorded on one to six occasions from each of a group of 18 conventional calves of the Friesian breed and 29 double-muscled calves of the Belgian White and Blue breed. Ages of the calves at the times of examination ranged from eight to 348 days. The Holmes semi-orthogonal lead system was used. The waves and interval durations and the tridimensional P, QRS, and T modal vector orientation and amplitude were calculated. The magnitude of the cardiac vectors was significantly lower and the ventricular waves and QT interval duration significantly shorter in the double-muscled than in the conventional calves. The P modal vector pointed significantly less downwards and the QRS modal vector pointed significantly more forwards and less up- and rightwards in the Belgian White and Blue, than in the Friesian group. Most of the observed differences might be a consequence of the bodily, and more specifically the thoracic, conformation of the former calves. However, the lower cardiac vector magnitude and shorter wave and interval durations might also reflect lower cardiac mass in the double-muscled subjects. [less ▲]

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See detailSex differences in fear reactions in sheep
Vandenheede, Marc ULg; Bouissou, M. F.

in Applied Animal Behaviour Science (1993), 37

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See detailEffects of Proglumide and Enprostil on Omeprazole-Induced Fundic Endocrine Cell Hyperplasia in Rats
Delwaide, Jean ULg; Latour, Pascale ULg; Gast, Pierrette ULg et al

in Gastroentérologie Clinique et Biologique (1993), 17(11), 792-6

Long-term treatment with omeprazole induces hyperplasia of enterochromaffin-like cells, closely related to hypergastrinemia. We studied whether proglumide, an antagonist of gastrin/CCK receptor, and ... [more ▼]

Long-term treatment with omeprazole induces hyperplasia of enterochromaffin-like cells, closely related to hypergastrinemia. We studied whether proglumide, an antagonist of gastrin/CCK receptor, and enprostil, a synthetic prostaglandin E2 derivative, might inhibit this hyperplasia. Six groups of 8 rats were treated for 10 weeks: a) untreated controls; b) omeprazole 10 mumol/kg; c) proglumide 500 mg/kg; d) enprostil 30 micrograms/kg; e) association of omeprazole and proglumide; f) association of omeprazole and enprostil. Serum gastrin levels were measured at different times during treatment. After sacrifice, fundic argyrophil cells were assessed by Grimelius' staining. Serum gastrin levels and argyrophil cell density were not modified in proglumide- and enprostil-treated groups, as compared with controls. Omeprazole increased significantly these two parameters. When given with omeprazole, proglumide decreased significantly serum gastrin levels and argyrophil cell density, as compared to omeprazole alone, while enprostil did not modify significantly these two parameters. These results indicate that proglumide, but not enprostil, can counteract the omeprazole-induced argyrophil cell hyperplasia in rats. [less ▲]

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See detailNeurotransmitter phenotype plasticity in adult dorsal root ganglia neurons
Moonen, Gustave ULg; Delrée, P.; Martin, Didier ULg et al

in Restorative Neurology & Neuroscience (1993), 5

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See detailReturn interval, firm size and systematic risk on the Dutch stock market
Corhay, Albert ULg; Tourani-Rad, Alireza

in Review of Financial Economics (1993), 2(2), 19-28

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See detailChiral theory of mesons in dense baryonic matter (II) : Meson propagation
Jaminon, Martine ULg; Mendez Galain; Stassart, Pierre ULg

in Nuclear Physics A (1993), 556

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See detailSynthetic model peptides for apolipoproteins. I. Design and properties of synthetic model peptides for the amphipathic helices of the plasma apolipoproteins.
Brasseur, Robert ULg; Vanloo, B.; Deleys, R. et al

in Biochimica et Biophysica Acta (1993), 1170(1), 1-7

Amphipathic helical peptides are the lipid-binding motives of the plasma apolipoproteins, and synthetic peptide analogs have been used to unravel the mechanism of lipid association within this class of ... [more ▼]

Amphipathic helical peptides are the lipid-binding motives of the plasma apolipoproteins, and synthetic peptide analogs have been used to unravel the mechanism of lipid association within this class of proteins. Hydrophobic interactions between the apolar amino acid residues belonging to the hydrophobic face of the amphipathic helices and the lipids are the major driving forces in the peptide-lipid association to form discoidal complexes. Ionic interactions and salt bridge formation between contiguous peptide chains in the complex can, however, contribute to the overall stability of the lipid-protein particle. This was studied by designing peptide analogs to the helical repeats of the apolipoproteins with variable degrees of salt bridge formation between adjacent peptide chains. The most stable conformation for pairs of synthetic peptides was calculated by energy minimisation together with the energy of interaction between peptides. The sequence of the peptides was derived from that of the 18A peptide synthesized by Segrest et al., and the theoretical calculations confirmed that ionic interactions between residues close to each other, along the edge of two adjacent anti-parallel peptides, can significantly contribute towards the stability of a peptide-phospholipid complex. [less ▲]

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See detailMonth-to-month variability of the general circulation fields in the western Mediterranean and the Gulf of Lions
Beckers, Jean-Marie ULg; Brasseur, P.

in Water Pollution Research (1993), 30

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See detailFollicular Dendritic Cells Do Not Produce Tnf-Alpha nor its Receptor
Mancini, I.; Bosseloir, A.; Hooghe-Peters, E. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailUltrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1993), 205(2), 430-2

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The ... [more ▼]

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleolar body until in close contact with the fibrillar centers. Morphometric analysis reveals that 32% of the fibrillar center surface is essentially occupied by condensed chromatin. Using the in situ terminal deoxynucleotidyl transferase-immunogold procedure for detecting DNA, we further show that evident label is exclusively found over the condensed chromatin and over the fibrillar centers, whereas no significant label is detected over the dense fibrillar component of the nucleolus. [less ▲]

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See detailApplying the Lagamine model to compute land subsidence in Shanghai
Dassargues, Alain ULg; Schroeder, Christian ULg; Li, Xiang Lin

in Bulletin of Engineering Geology & the Environment (1993), 47

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See detailEffect of prolactin on alpha and beta chloride cells in the gill epithelium of the saltwater adapted tilapia " Oreochromis niloticus"
Pisam, B.; Auperin, B.; Prunet, P. et al

in Anatomical Record (1993), 235

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the ... [more ▼]

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the latter, fishes were injected every 2 days for a week with tilapia prolactin (ti-PRL I). Gills were prepared for electron microscopy in order to determine the types and surface areas of chloride cells in each experimental condition. Two types of chloride cells, the alpha and beta cells were easily distinguished on the basis of their location and ultrastructural features in the gills of freshwater fishes, while only one type of cell, the saltwater alpha cells presumably derived from the transformation of the freshwater alpha cells, were encountered in saltwater adapted animals. After PRL injection of saltwater adapted fishes, small chloride cells, which displayed ultrastructural features similar to those of beta cells in freshwater tilapia, reappeared in interlamellar regions of the gills. In the same experimental conditions, the voluminous saltwater alpha cells showed a tendency to resume ultrastructural features more characteristic of the freshwater alpha cells from which they were derived. These observations tend to indicate that prolactin behaves as a "freshwater adapting hormone" and that beta cells are specifically involved in fish adaptation to freshwater living conditions. [less ▲]

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See detailAnalytical calculation of the electrostatic interaction energy within the CNDO framework
Dehareng, Dominique ULg; Dive, Georges ULg; Ghuysen, Jean-Marie ULg

in International Journal of Quantum Chemistry (1993), 46(6), 711-734

This work analyses the adequacy of an analytical electrostatic energy formulation within the CNDO framework to predict the stable conformations of large molecular complexes. Comparisons are made with ab ... [more ▼]

This work analyses the adequacy of an analytical electrostatic energy formulation within the CNDO framework to predict the stable conformations of large molecular complexes. Comparisons are made with ab initio results for small systems such as water-formamide, methanol-water-imidazole, or guanine-cytosine and with AM1 results for two large systems: a molecular tweezer + the 9-methyladenine complex and a model active site of the alpha-chymotrypsin and its ligand complex. This approach is efficient in providing reliable conformers for large molecular systems in a very fast way. [less ▲]

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See detailDes inscriptions de sceaux-cylindres diverses provenant d'Acemhöyük
Tunca, Önhan ULg

in Aspects of Art and Iconography: Anatolia and its Neighbors. Studies in Honor of Nimet Özgüç (1993)

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See detailEnzymatic hydrolysis of reconstituted dimyristoylphosphatidylcholine-apo A-I complexes.
Lins, Laurence ULg; Piron, S.; Conrath, K. et al

in Biochimica et Biophysica Acta (1993), 1151(2), 137-42

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form ... [more ▼]

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form discoidal particles of defined dimensions. While the knowledge of the apo A-I sequence and secondary structure has been used to make predictions about its mode of association with lipids, the available experimental data necessary to propose a precise model of these discoidal structures are still limited. An important step in our understanding of these structures would be to identify the apolipoprotein lipid-associated domains. Proteolysis of apo A-I-DMPC reconstituted HDL (rHDL) and free apo A-I is used here to identify lipid-protected domains of apo A-I. Free cleaved peptides were separated from rHDL associated peptides by density gradient centrifugation. The lipid-associated peptides were further analyzed by SDS-PAGE and transferred by Western blot to a ProBlott membrane for sequencing. Cleavage occurred at residue 43 with proteinase K, 46 with trypsin and residue 47 or 48 with pronase. A large domain from about residue 45 to the C-terminal remains highly protected against hydrolysis eventhough it contains several bonds susceptible to proteolytic cleavage. No protected fragments were detected by SDS-PAGE after enzymatic cleavage of free apo A-I in identical experimental conditions. [less ▲]

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See detailSoluble CD23 and other receptors (CD4, CD8, CD25, CD71) in serum of patients with chronic lymphocytic leukemia.
Beguin, Yves ULg; Lampertz, S.; De Groote, D. et al

in Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K (1993), 7(12), 2019-2025

We measured the soluble (s) receptors CD23, CD8, CD4, interleukin-2 receptor (IL-2R, CD25), and transferrin receptor (TfR, CD71), in normal serum and in patients with chronic lymphocytic leukemia (CLL ... [more ▼]

We measured the soluble (s) receptors CD23, CD8, CD4, interleukin-2 receptor (IL-2R, CD25), and transferrin receptor (TfR, CD71), in normal serum and in patients with chronic lymphocytic leukemia (CLL) and evaluated them in relation to clinical and biological parameters of the disease, as well as serum immunoglobulin E (IgE). Compared to 31 normal individuals, 42 CLL patients had increased levels of sCD23 (98.4 +/- 127.7 versus 0.9 +/- 0.3 U/ml, p < 0.001), sIL-2R (6080 +/- 7030 versus 1420 +/- 640 pg/ml, p < 0.001), sTfR (12,100 +/- 11,250 versus 5000 +/- 1050 ng/ml, p < 0.001), and sCD8 (510 +/- 191 versus 234 +/- 89 U/ml, p < 0.001), but normal sCD4 levels. Mean sCD23 levels remained normal in patients with non-Hodgkin's lymphoma (other than small lymphocytic), Hodgkin's disease, hairy cell leukemia, acute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML), multiple myeloma, or solid tumors. Advancing Rai clinical stage was associated with a progressive elevation of sCD23 (p < 0.001), while sCD8 (p < 0.05), sIL-2R (p < 0.001), and sTfR (p < 0.005) were highest in stage 2 patients. Discriminant analysis confirmed the value of soluble receptor determinations in the clinical evaluation of CLL patients. sCD23 correlated with sIL-2R (p < 0.001) and sTfR (p < 0.05) but not with sCD4 or sCD8, and displayed an inverse relationship with serum IgE (NS) and total gamma-globulin (p < 0.05). sIL-2R correlated with sCD23 (p < 0.001), sTfR (p < 0.001), sCD4 (p < 0.01), and sCD8 (p < 0.01). The lymphocyte count correlated with serum lactate dehydrogenase (LDH) (p < 0.05), sCD23 (p < 0.001) and sIL-2R (p < 0.01) but not sTfR, sCD8, or sCD4. Chemotherapy produced consistent reductions of sCD23 levels in two responding patients. We conclude that: (i) sCD23 is considerably elevated in CLL, correlates with the tumor mass and clinical stage, and could be helpful in monitoring these patients; and (ii) sIL-2R, sCD8, and sTfR levels are less specifically increased and could be influenced by other factors such as immune activation and erythropoiesis. [less ▲]

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See detailNote sur les techniques de gravure des sceaux-cylindres en Asie antérieure
Tunca, Önhan ULg

in Technologie et analyse des gemmes anciennes (PACT 23) (1993)

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See detail"Influence of bioreactor aeration conditions on sporulation rate of Bacillus subtilis (b 213 stream)"
Meurisse, E.; Hbid, Ch.; Jacques, Ph. et al

in Mededelingen - Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent (1993), 58(4b), 1985-1987

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See detailBovine leukemia virus, an animal model for the study of intrastrain variability.
Willems, Luc ULg; Thienpont, E.; Kerkhofs, P. et al

in Journal of Virology (1993), 67(2),

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See detailAspects cliniques de la neoplasie endocrinienne multiple de type 1 (NEM-1).
Ghuysen, Alexandre ULg; Stevenaert, Achille ULg; Beckers, Albert ULg

in Revue Médicale de Liège (1993), 48(6), 309-63

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See detailEl analisis estructural semi-rigido: definicion, camp y mood de applicacion
Jaspart, Jean-Pierre ULg; Maquoi, René ULg

in Construcciones Metalicas y Caldereria (1993), 28

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See detailMathematical Programming Methods for Constrained Optimization: Dual Methods
Fleury, Claude ULg

in Progress in Astronautics and Aeronautics (1993), 150

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See detailPotentialités de modulation de la réaction inflammatoire
Delannoy, I.; Miossec, P.; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (1993), 137

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See detailSmall Bowel Enema and Diagnosis of Chronic Nonischemic Disturbance of Superior Mesenteric Venous Blood Flow
Boverie, J. H.; COUNET, D.; Meunier, Paul ULg et al

in Abdominal Imaging (1993), 18(3), 265-70

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis ... [more ▼]

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis and chronic thrombotic forms with portal hypertension or hypercoagulopathy. In eight patients this syndrome was secondary to organic lesions of different origin: mesenteric vein squeezed by fibrous bands or an abnormal jejunal artery (four cases), lymphoma involving the distal superior mesenteric veins (three cases), hemangioma causing microthrombi (one case). In three patients no etiology or predisposing factor was found. All patients presented with rectal hemorrhage. Small bowel enema showed a constant pattern in 11 patients: small nodules, modified by compression or peristalsis, involving the mesenteric border of the jejunoileal segment, and associated with thick, straight but regular folds. Mesenteric varices were suspected and led to angiographic studies which were normal in three cases, confirmed varices in eight cases, and thrombosis in four cases. Laparotomy was normal in three cases and established the etiological diagnosis in eight cases. Varices were shown in six cases. Arteriography and laparotomy were unable to reach a complete diagnosis. [less ▲]

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See detailA VARIABLE MECHANISM FOR THE NUCLEOPHILIC VINYLIC SUBSTITUTIONS IN A SERIES OF GEM-DIHALOGENATED ALKENES BY A BIDENTATE SULFUR NUCLEOPHILE - AN EXPERIMENTAL AND AM1 THEORETICAL-STUDY
Gimbert, Yves; Moradpour, Alec; Dive, Georges ULg et al

in Journal of Organic Chemistry (1993), 58(17), 4685-4690

The nucleophilic substitutions of a series of gem-dihalogenated alkenes 3,5,7,8, and 9 (RS)2C=CX2 (X = Cl, F) with 1,2-benzenedithiolate b have been studied. Depending on the structures of the R groups ... [more ▼]

The nucleophilic substitutions of a series of gem-dihalogenated alkenes 3,5,7,8, and 9 (RS)2C=CX2 (X = Cl, F) with 1,2-benzenedithiolate b have been studied. Depending on the structures of the R groups (alkyl, saturated and unsaturated cycloalkyls, aromatic ring), the course of the reactions and the structures of the yielded products are modified. In the frame of the addition-elimination-type mechanism for these nucleophilic substitutions, the energy contents of the anionic intermediates, resulting from the additions of the nucleophile b to the unsaturated centers, is calculated at the AM1 method level. For the compounds 5, 7, 8, and 9, the calculated energies nicely corroborate the experimental results. For 3, anionic intermediates are no longer found by calculation, and a synchronous single-step substitution is strongly suggested. [less ▲]

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See detailEtude de l'effet de 2 additifs à base d'extrait de Yucca sur la réduction des émissions d'azote ammoniacal par des lisiers de porcs
Marlier, Didier ULg; Nicks, Baudouin ULg; Canart, Bernard

in Annales de Médecine Vétérinaire (1993), 137(7), 485-493

The effects of two commercial yucca extracts on the ammonia release from pig slurry have been tested in laboratory and in one fattening house. In laboratory, tests were carried on with 4 litre samples ... [more ▼]

The effects of two commercial yucca extracts on the ammonia release from pig slurry have been tested in laboratory and in one fattening house. In laboratory, tests were carried on with 4 litre samples whose ammonia production was recorded during 35 days. One of the two products, at the factory recommanded dose of 5 g/l, has successfully lowered the ammonia emission rate of the slurry. In the pig house, the two same products were given in the pigs' diet at the dose of 160 and 200 g/ton of feed. Inside a same building, pigs of one room received feed plus yucca extracts and those of the second room feed alone; none of the 2 products has significantly reduced the ammonia concentrations recorded on a three month period. One can expect improvements of the yucca extracts but up to now no guaranty can be given of their efficiency. [less ▲]

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See detailApamin increases NMDA-induced burst-firing of rat mesencephalic dopamine neurons.
Seutin, Vincent ULg; Johnson, S. W.; North, R. A.

in Brain Research (1993), 630(1-2), 341-4

Intracellular recordings made in vitro from rat midbrain dopamine neurons showed that apamin (100 nM) did not alter the regular spontaneous firing of the neurons, but it increased the occurrence of bursts ... [more ▼]

Intracellular recordings made in vitro from rat midbrain dopamine neurons showed that apamin (100 nM) did not alter the regular spontaneous firing of the neurons, but it increased the occurrence of bursts of action potentials in N-methyl-D-aspartate. Apamin appeared to facilitate burst-firing induced by NMDA because, by blocking an outward calcium-activated potassium current, it increased the depolarizing action of NMDA. [less ▲]

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See detailMicroclimat et composition de l'air dans une enceinte fermée destinée à l'étude de la toxicité des polluants atmosphériques chez le porcelet
Urbain, B.; Gustin, Pascal ULg; Prouvost, J. F. et al

in Veterinary Research (1993), 24(6), 503-514

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate ... [more ▼]

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate, total dust, total viable particles, endotoxins in respirable dust (< 5 microns) and ammonia were measured in a 1.9 m3 environmental chamber designed for piglets. The activity of 1 piglet was recorded on video. Temperature and relative humidity were respectively 23.9 +/- 1.3 degrees C and 70.5 +/- 8.3% (mean +/- SD). Air flow rate was 10 m3/h. Dust, viable particles, endotoxin and ammonia concentrations were respectively 7 +/- 2 particles/ml, 3.4 x 10(4) +/- 2.9 x 10(4) BCFP (bacterial colony-forming particles)/m3, 4.8 +/- 1.5 ng/m3 and 6 +/- 0.8 ppm (mean +/- SD). All these parameters were comparable to the lowest values recorded in pigpens [less ▲]

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See detailLes formes normes d’ordres,
Pays, Isabelle ULg

in Journal of Algebra (1993), 155

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See detailHigh Resolution Frequency Analysis as applied to the singing voice
Morsomme, Dominique ULg; Remacle, Marc; Millet, Benoite

in Folia Phoniatrica (1993), 45

We have applied high-resolution vocal frequential analysis to a population of singing voices. Two important elements have become apparent: (1) Confirmation that the singing formant originates in the ... [more ▼]

We have applied high-resolution vocal frequential analysis to a population of singing voices. Two important elements have become apparent: (1) Confirmation that the singing formant originates in the resonators. This is observed especially on a low fundamental, and it is acquired through technical skill and experience (2)Observation of the vibrato, which, isolated from the clinical study, regarding only its graphic presentation, could habe been interpreted as "abnormal". [less ▲]

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See detailPREFERENTIAL DEACTIVATION OF THE S(3) STATE OF THE WATER-OXIDIZING COMPLEX, FAVORED BY PLASTOQUINONE REDUCTION IN BARLEY CHLOROPLASTS
Franck, Fabrice ULg; SCHMID, G. H.

in Zeitschrift Fur Naturforschung C-A Journal Of Biosciences (1993), 48(7-8), 603-608

The flash patterns of oxygen evolution after pre-illumination by continuous light or by a flash sequence were compared in etiochloroplasts and mature chloroplasts of barley. In both types of plastids sub ... [more ▼]

The flash patterns of oxygen evolution after pre-illumination by continuous light or by a flash sequence were compared in etiochloroplasts and mature chloroplasts of barley. In both types of plastids sub-saturating continuous illumination of some seconds strongly affected the S3 but not the S2 state of the oxygen-evolving complex. This result is ascribed to efficient charge recombination of S3 with the acceptor side of photosystem II, favoured by partial reduction of the plastoquinone pool. The increase of S3 observed in the presence of dichlorophenolindophenol in etiochloroplasts confirms this interpretation. These observations strengthen the recent hypothesis of a conformational change during the transition from S2 to S3 recently proposed to interpret the different susceptibility of these two states to hydroxylamine and hydrazine (F. Frank and G. H. Schmid, Biochim. Biophys. Acta 977, 215-218 (1989); J. Messinger, U. Wacker, and G. Renger, Biochemistry 30,7852-7862 (1991)). [less ▲]

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See detailInfluence of body size on tibial nerve somato sensory evoked potentials in dogs
Poncelet, Luc; Michaux, Charles; Balligand, Marc ULg

in American Journal of Veterinary Research (1993)

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See detailCreativity in Non-Literary Translation
Pagnoulle, Christine ULg

in Perspectives : Studies in Translatology (1993), (1), 79-90

Three short non literary texts illustrate the need for translators' actively stepping in. Since the author is both a translator and a teacher of translation didactic conclusions are also drawn.

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See detailStructure des populations de renard roux, Vulpes vulpes, dans le sud de la Belgique en relation avec l'enzootie rabique et les campagnes de vaccination orale
AFIADEMANYO, Komlan; Libois, Roland ULg; Brochier, Bernard et al

in Cahiers d'Ethologie (1993), 13(3), 281-294

From 1989 to 1991, 549 fox carcasses were examined in oder to assess the impact of rabies control measures (oral vaccination with VVTGgRAB recombinant vaccinia-rabies virus). Their age was determined ... [more ▼]

From 1989 to 1991, 549 fox carcasses were examined in oder to assess the impact of rabies control measures (oral vaccination with VVTGgRAB recombinant vaccinia-rabies virus). Their age was determined using a lower canine tooth. The distinction between young and adults was made on radiographs, measuring the relative importance of the pulp cavity. Adult animals were aged by counting the annual growth cementum layers in tooth sections. The sex-ratio is in favour of the males and the age-pyramid is a large-base flattened one, with more than 60% of young (< 1 year) animals. Life expectancy at birth is 1.14 y. in the males and 1.23 y. in the females. The population turnover is quite high, the whole population being revewed in a 3-4 y. time interval. As far as the age structure is concerned, no difference has been found between males and females, rabid and sound animals, immunised or not. However, the spring vaccination campaign is much less efficient for the young foxes. These results suggest that rabies affects indistinctly all age classes of both sexes, just as it is the case with the autumn vacciation campaigns. Moreover, a comparison between the samples of two natural regions differing in their hunting system (big vs. small game) revealed no age structure difference suggesting that the high hunting pressure can explain better than rabies itself the very important juvenile prportion in the population. Nevertheless, some further research is needed to confirm that last point. [less ▲]

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See detailEfficient lipofection of human trophoblast cells in primary cultures
Jacquemin, P.; Alsat, E.; Oury, Cécile ULg et al

in Biochemical and Biophysical Research Communications (1993), 196(1), 376-81

Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this hormone ... [more ▼]

Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this hormone, we became interested in transfecting primary cultures of these trophoblast cells. In this study, we show that it is possible to transfect, by the lipofection method, these giant cells in an efficient and reproducible manner. We show the presence of an enhancer region downstream from the hCS-B gene, functionally active in these cells; furthermore, we demonstrate the placenta-specific characteristic of this enhancer, previously identified in a human choriocarcinoma cell line. [less ▲]

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See detailAspects cliniques de la néoplasie endocrinienne multiple de type 2 (NEM-2)
Moreau, L.; Beckers, Albert ULg

in Médecine & Chirurgie Digestives (1993), 23(1), 17-20

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See detailLes noms belgoromans de la rougeole. Une étude de géographie linguistique
Boutier, Marie-Guy ULg

in Dialectes de Wallonie (1993), 21/22

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See detailRésultats de la mesure de la concentration en poussière dans l'air de 12 porcheries
Marlier, Didier ULg; Nicks, Baudouin ULg; Canart, Bernard

in Annales de Médecine Vétérinaire (1993), 137

Aerial dust concentrations in 12 pig houses Dust concentration was measured during 3 days at hourly intervals in 5 farrowing houses and 7 fattening houses. The number concentration of aerial particles was ... [more ▼]

Aerial dust concentrations in 12 pig houses Dust concentration was measured during 3 days at hourly intervals in 5 farrowing houses and 7 fattening houses. The number concentration of aerial particles was measured using a counter which sizes the total numbers in 5 categories: from 0.3-0.5 mum to > 5 mum. The mean dust concentrations were significantly higher in the farrowing houses than in the fattening houses (179 +/- 91 vs 97 +/- 65 particles/ml). Over a 24-h period, the ratio between the highest and the lowest hourly concentrations was 3 +/- 1 in the farrowing houses and 5 +/- 4 in the fattening houses; the difference between the 2 types of houses is significant. The smallest particles were the most numerous; 82 % of the aerial particles were in the size range 0.3-0.5 mum, with less than 1 % being 5 mum or larger. During the most polluted hours, there was proportionally more particles in the size range 0.5-1 mum and significantly less particles in the size ranges 1-2 mum and 2-5 mum, as compared to the less polluted hours. This study shows that most of the aerial particles in piggeries are smaller than 5 mum, the size below which deposition is regarded to occur in the lower respiratory tract, the farrowing houses being more polluted than the fattening houses. [less ▲]

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See detailLong-Term Photometry of Variables at ESO. II. The Second Data Catalogue (1986-1990).
Sterken, C.; Manfroid, Jean ULg; Anton, K. et al

in European Southern Observatory Scientific Report (1993), 12

Not Available

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See detailBiometrical evaluation of the stratum corneum texture in patients under maintenane hemodialysis
Deleixhe-Mauhin, F.; Pierard, Claudine ULg; Krzesinski, Jean-Marie ULg et al

in Nephron (1993), 64

Xerosis and hydration of the stratum corneum were evaluated in 60 hemodialyzed patients. Xerosis and a low capacitance of the stratum corneum were evidenced in more than 80% of the patients. Pruritus was ... [more ▼]

Xerosis and hydration of the stratum corneum were evaluated in 60 hemodialyzed patients. Xerosis and a low capacitance of the stratum corneum were evidenced in more than 80% of the patients. Pruritus was present in two third of the subjects. We failed to disclose any significant relationship between severity of these three parameters. [less ▲]

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See detailComputed subsidence of the central area of Shanghai
Dassargues, Alain ULg; Radu, Jean-Pol ULg; Charlier, Robert ULg et al

in Bulletin of Engineering Geology & the Environment (1993), 47

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See detailDifferent Mechanisms of Extracellular Matrix Remodeling by Fibroblasts in Response to Human Mammary Neoplastic Cells
Noël, Agnès ULg; Munaut, Carine ULg; Nusgens, Betty ULg et al

in Invasion & Metastasis (1993), 13(2), 72-81

Human breast tumors are often associated with a fibrotic reaction termed desmoplasia. Tumor cells may indirectly modulate the composition of the extracellular matrix by influencing fibroblast properties ... [more ▼]

Human breast tumors are often associated with a fibrotic reaction termed desmoplasia. Tumor cells may indirectly modulate the composition of the extracellular matrix by influencing fibroblast properties. They may also directly interact with collagen fibrils leading to retraction of the matrix. We have studied in vitro the influence of various human mammary tumor cells on the proliferation rate of normal human fibroblasts and on their level of collagen synthesis, as well as their release of collagenase activity. Interactions between neoplastic cells and collagen matrix were investigated by incorporation of tumor cells in collagen gels (lattices) and measurement of their retraction. All cells tested (HBL100, SW613, SA52, MDA-MB-231, MCF7, MCF7/6, MCF7 ras, BT20 and T47D) were able to modulate the composition of the extracellular matrix by one or several of the mechanisms investigated. Our results also demonstrate an opposite regulation of collagen and collagenase production. The effects on the collagen metabolism and on fibroblast proliferation are probably mediated by soluble cytokines since they are reproduced by incubating the fibroblasts in the presence of medium conditioned by tumor cells. The desmoplastic reaction may thus result from different mechanisms dependent upon tumor cell types. [less ▲]

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See detailEvaluation isocinétique des genoux ligamentaires en pré et postopératoire
Croisier, Jean-Louis ULg; Delcour, J. P.; Dupont, Géraldine ULg et al

in Lettre de Médecine Physique et de Réadaptation (1993), 29

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See detailNitric oxide: a new messenger in the brain.
Bruhwyler, J.; Chleide, E.; Liégeois, Jean-François ULg et al

in Neuroscience & Biobehavioral Reviews (1993), 17(4), 373-84

The important role played by nitric oxide (NO) in the central nervous system has largely been emphasized in the recent literature. It can originate at least from four different sources: the endothelium of ... [more ▼]

The important role played by nitric oxide (NO) in the central nervous system has largely been emphasized in the recent literature. It can originate at least from four different sources: the endothelium of cerebral vessels, the immunostimulated microglia and astrocytes, the nonadrenergic noncholinergic nerve, and the glutamate neuron. NO has been implicated in a large number of pathologies (such as neurotoxicity in Alzheimer's disease and Huntington's disease, cerebral ischemia, stroke, and anxiety) and also in normal physiological functions (such as memory and learning, regulation of the cerebrovascular system, modulation of the wakefulness, mediation of nociception, olfaction, food intake and drinking, regulation of noradrenaline, and dopamine release). The aim of this paper is to review and to integrate the most recent advances in our understanding of the roles of NO in the brain. [less ▲]

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See detailPotato chloroplasts express an intron-encoded maturase-like polypeptide
du Jardin, Patrick ULg; Portetelle, Daniel ULg; Harvengt, L. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1993), 101

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See detailL'éducation, un acte fondamental
de Landsheere, Gilbert ULg

in Réseaux : Revue Interdisciplinaire de Philosophie Morale et Politique (1993), (67-69), 65-81

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See detailTemperature, air humidity and air pollution levels in breeding pig houses
Nicks, Baudouin ULg; Canart, B.; Vandenheede, Marc ULg

in Pigs News and Information (1993), 14(2), 77-78

A study was undertaken to provide practical information concerning the frequency of temp. and RH measurements required to obtain a good estimate of their averages and of their daily variations, and to ... [more ▼]

A study was undertaken to provide practical information concerning the frequency of temp. and RH measurements required to obtain a good estimate of their averages and of their daily variations, and to determine the relationships between ammonia, carbon dioxide and bacterial concn in a pig house. Measurements were made in 9 artificially ventilated farrowing or weaner houses with slatted floors. Temp. and RH were measured every 20 min for 7 d. Sensors were placed inside buildings in the middle of the houses, 0.8 m above floor level, and outside the buildings. Data were recorded using a datalogger. Ammonia and carbon dioxide concn were measured using 8 h Gastec diffusion tubes. Three readings were taken in the same location at 2-day intervals. The airborne bacterial concn was measured with a 6-stage Andersen sampler. Bacteria colony forming particles (BCFP) were collected by impacting onto agar-filled petri dishes which are placed in an incubator for 24 h prior to counting the colonies formed. Results are discussed, indicating no significant correlation between the 3 measures of pollution (NH3 and CO2 concn, BCFP levels) despite careful microclimate control. [less ▲]

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See detailFollicular dendritic cells: isolation procedures, short and long term cultures.
Heinen, Ernst ULg; Tsunoda, R.; Marcoty, C. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailMutations in type 1 procollagen that cause osteogenesis imperfecta: effects of the mutations on the assembly of collagen into fibrils, the basis of phenotypic variations, and potential antisense therapies.
Prockop, D. J.; Colige, Alain ULg; Helminen, H. et al

in Journal of Bone and Mineral Research (1993), 8 Suppl 2

Work by a large number of investigators over the last decade has established that over 90% of patients with osteogenesis imperfecta have mutations in one of the two genes for type I procollagen, that most ... [more ▼]

Work by a large number of investigators over the last decade has established that over 90% of patients with osteogenesis imperfecta have mutations in one of the two genes for type I procollagen, that most unrelated probands have different mutations in the genes, and that the mutations found in most of the serious variants of the disease cause synthesis of abnormal pro alpha chains of the protein. The results have demonstrated that synthesis of structurally abnormal but partially functional pro alpha chains can interfere with folding of the central region of the protein into a triple-helical conformation, prevent processing of the N-terminal propeptides of procollagen, or produce subtle alterations in conformation that interfere with the self-assembly of the protein into collagen fibrils. One of the unsolved mysteries about the disease is why some mutations produce severe phenotypes, whereas very similar mutations produce mild phenotypes. Recent studies in transgenic mice suggest that nongenetic factors, such as stochastic events during development, may determine the severity of the disease phenotype produced by a specific mutation. Also, recent results raised the possibility that strategies of antisense gene therapy may be effective in treating the disease some time in the future. Specific inhibition of expression of a mutated collagen gene has been obtained with antisense oligonucleotides in cell culture experiments. However, there is no means of selective delivery of antisense oligonucleotides to the appropriate tissues. [less ▲]

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See detailCloning and characterization of G protein-coupled receptors
Parmentier, M.; Libert, F.; Perret, J. et al

in Advances in Second Messenger and Phosphoprotein Research (1993), 28

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See detailTemporal reasoning and reasoning theories : a case study in anaesthesiology
Sougné, Jacques ULg; Nyssen, Anne-Sophie ULg; De Keyser, Véronique ULg

in Psychologica Belgica (1993), 33(2), 311-328

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that ... [more ▼]

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that appear in working situations and even, understanding and preventing some human errors. In order ot illustrate theoritical concepts, we will provide examples of a situation in which the dynamic character of the evolution, the critical character of the planning and of the synchronization force temporal reasoning. This situation will be that of anaesthesia. [less ▲]

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See detailBiological control of postharvest Botrytis cinerea and Penicillium on apples
Jijakli, Haissam ULg; Lepoivre, Philippe ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (1993), (16), 106-110

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See detailStructure sociologique de l'élecotrat écologiste en Wallonie : une première exploration
Jacquemain, Marc ULg; Vandekeere, Michel; Doutrelepont, René

in Res Publica (1993), 25

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much ... [more ▼]

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much more educated, e.a. [less ▲]

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See detailLe nom du bison en grec et en latin
Purnelle, Gérald ULg

in Latomus : Revue d'Etudes Latines (1993), 52(3), 567-580

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See detailEvolutionary relationships among higher fungi inferred from small ribosomal subunit RNA sequence analysis
Wilmotte, Annick ULg; Van De Peer, Yves; Goris, Anne et al

in Systematic & Applied Microbiology (1993), 16

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The ... [more ▼]

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The sequences were fitted into an alignment of all hitherto published complete or nearly complete eukaryotic small subunit rRNA sequences. The evolutionary relationships within the fungi were examined by construction of a tree from 87 SSU rRNA sequences, corresponding to 71 different species, by means of a distance matrix method and bootstrap analysis. It confirms the early divergence of the zygomycetes and the classical division of the higher fungi into basidiomycetes and ascomycetes. The basidiomycetes are divided into true basidiomycetes and ustomycetes. Within the ascomycetes, the major subdivisions hemiascomycetes and euascomycetes can be recognized. However, Schizosaccharomyces pombe does not belong to the cluster of the hemiascomycetes, to which it is assigned in classical taxonomic schemes, but forms a distinct lineage. Among the euascomycetes, the plectomycetes and the pyrenomycetes can be distinguished. Within the hemiascomycetes, the polyphyly of genera like Pichia or Candida and of families like the Dipodascaceae and the Saccharomycetaceae can be observed. [less ▲]

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See detailPHOTOREDUCTION OF PROTOCHLOROPHYLLIDE TO CHLOROPHYLLIDE IN 2-D-OLD DARK-GROWN BEAN (PHASEOLUS-VULGARIS CV COMMODORE) LEAVES - COMPARISON WITH 10-D-OLD DARK-GROWN (ETIOLATED) LEAVES
SCHOEFS, B.; Franck, Fabrice ULg

in Journal of Experimental Botany (1993), 44(263), 1053-1057

Two-d-old leaves which do not contain prolamellar bodies synthesize active protochlorophyllide in darkness. When protochlorophyllide is photoreduced by one intense white flash, a main chlorophyllide ... [more ▼]

Two-d-old leaves which do not contain prolamellar bodies synthesize active protochlorophyllide in darkness. When protochlorophyllide is photoreduced by one intense white flash, a main chlorophyllide species emitting at 690 nm is formed. After the photoreduction, the emission maximum is shifted to 675 nm within 5 s. This result suggests that in young leaves, chlorophyllide formed after one flash is quickly released from the active site of NADPH: protochlorophyllide oxidoreductase. This interpretation is strenghtened by time-resolved fluorescence measurements at room temperature, showing that 675 nm emitting chlorophyllide does not transfer excitation energy to the 696 nm emitting chlorophyllide which is formed in very low amount. In 10-d-old bean leaves, the 690 nm chlorophyllide emitting species formed after one short flash undergoes the well-known rapid and Shibata spectral shifts. The 675 nm emitting chlorophyllide appears only as a shoulder. At both ages, the fluorescence intensity of the active protochlorophyllide strongly decreases during and after photoreduction, suggesting rapid modifications in the close environment of the pigment. [less ▲]

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See detailSell-Off en de welvaart van aandeelhouders
Corhay, Albert ULg; Tourani-Rad, Alireza

in Maandblad voor Accountancy en Bedrijfseconomie [=MAB] (1993), 67(10), 471-477

In het kader van de herstructurering van het Nederlandse bedrijfsleven vindt de laatste jaren een groot aantal Sell-Offs plaats. Theoretici betogen dat een Sell-Off een aantal voordelen voor het ... [more ▼]

In het kader van de herstructurering van het Nederlandse bedrijfsleven vindt de laatste jaren een groot aantal Sell-Offs plaats. Theoretici betogen dat een Sell-Off een aantal voordelen voor het afstotende moederbedrijf oplevert. Blijkens deze studie ondersteunt het empirische onderzoek de theorie. In Nederland lijken significante buitengewone positieve koerseffecten op de dag van aankondiging voor de aandeelhouders van de verkopende bedrijven met dit soort transacties gepaard te gaan. Tevens is vastgesteld dat de aard van de aankondiging en de bekendmaking van het motief voor de Sell-Off positieve significante invloed heeft op de grootte van het buitengewoon rendement. Ten slotte is geconcludeerd dat alleen in het geval van buiten Nederland gevestigde dochters sprake is van een positief significant rendement [less ▲]

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See detailSubjective shortening in human's memory for stimulus duration
Wearden, J. H.; Ferrara, André ULg

in Quarterly Journal of Experimental Psychology (1993), 46B(2), 163-186

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See detailExiste-t'il une Europe de l'épargne?
Pestieau, Pierre ULg; Perelman, Sergio ULg

in Reflets et Perspectives de la Vie Economique (1993), (32), 37-59

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See detailFlexible Technique for Multiple Organ Procurement in Rats: A Training and Research Tool in Transplantation
D'Silva, M.; Pirenne, J.; Bonnet, Pierre ULg et al

in Microsurgery (1993), 14(5), 342-9

Multiple organ procurement techniques are increasingly popular in many transplant centers. This paper describes a technique for multiorgan unit(s) explantation in rats. The procedure involves in situ ... [more ▼]

Multiple organ procurement techniques are increasingly popular in many transplant centers. This paper describes a technique for multiorgan unit(s) explantation in rats. The procedure involves in situ preliminary dissection, cold perfusion of the thoracic and abdominal organ units as separate procedures, explantation, and finally the ex situ isolation of various organ units as single and/or multiple organ combination units. The procedure is feasible, allows for flexibility in the separation of combination organ units, and is reproducible. The exercise is provided as a training and research tool for clinical and research transplant surgeons. The technique is also presented as an animal model for clinical organ sharing. [less ▲]

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See detailInventaire des végétaux éodévoniens de Belgique
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1993), 116

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See detailThiamine Triphosphate Activates an Anion Channel of Large Unit Conductance in Neuroblastoma Cells
Bettendorff, Lucien ULg; Kolb, Hans-Albert; Schoffeniels, E.

in Journal of Membrane Biology (1993), 136(3), 281-8

In neuroblastoma cells, the intracellular thiamine triphosphate (TTP) concentration was found to be about 0.5 microM, which is several times above the amount of cultured neurons or glial cells. In inside ... [more ▼]

In neuroblastoma cells, the intracellular thiamine triphosphate (TTP) concentration was found to be about 0.5 microM, which is several times above the amount of cultured neurons or glial cells. In inside-out patches, addition of TTP (1 or 10 microM) to the bath activated an anion channel of large unit conductance (350-400 pS) in symmetrical 150 mM NaCl solution. The activation occurred after a delay of about 4 min and was not reversed when TTP was washed out. A possible explanation is that the channel has been irreversibly phosphorylated by TTP. The channel open probability (Po) shows a bell-shaped behavior as a function of pipette potential (Vp). Po is maximal for -25 mV < Vp < 10 mV and steeply decreases outside this potential range. From reversal potentials, permeability ratios of PCl/PNa = 20 and PCl/Pgluconate = 3 were estimated. ATP (5 mM) at the cytoplasmic side of the channel decreased the mean single channel conductance by about 50%, but thiamine derivatives did not affect unit conductance; 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (0.1 mM) increased the flickering of the channel between the open and closed state, finally leading to its closure. Addition of oxythiamine (1 mM), a thiamine antimetabolite, to the pipette filling solution potentiates the time-dependent inactivation of the channel at Vp = -20 mV but had the opposite effect at +30 mV. This finding corresponds to a shift of Po towards more negative resting membrane potentials. These observations agree with our previous results showing a modulation of chloride permeability by thiamine derivatives in membrane vesicles from rat brain. [less ▲]

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See detailChylothorax: a rare complication of endoscopic variceal sclerotherapy.
Bury, Thierry ULg; Corhay, Jean-Louis ULg; Louis, Renaud ULg et al

in European Journal of Gastroenterology & Hepatology (1993), 5

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See detailVers la postmodernité
Badir, Sémir ULg

in Ecritures (1993), 5

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See detailA finite element code for subsidence problems: LAGAMINE
Charlier, Robert ULg; Radu, Jean-Pol ULg; Li, Q. F.

in Bulletin of Engineering Geology & the Environment (1993), 47(1 / avril), 5-11

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See detailCharacterization of the negative regulatory protein PHO80 involved in transcriptional regulation of PHO5 in Saccharomyces cerevisiae
Jacqmin, S.; Gilliquet, V.; Portetelle, Daniel ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1993), 101

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See detailMapping Of B-Neutralizing And T-Helper Cell Epitopes On The Bovine Leukemia-Virus External Glycoprotein Gp51
Callebaut, I.; Voneche, V.; Mager, A. et al

in Journal of Virology (1993), 67(9),

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See detailLes colibacilloses dans l'espèce bovine
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (1993), 137

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See detailIdentification of eae genes in rabbit attaching and effacing enteropathogenic Escherichia coli (EPEC)
Pohl, P.; Peeters, J. E.; Jacquemin, E. R. et al

in Infection and Immunity (1993), 61

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See detailDe l'emploi des tests dans l'expertise psychologique
Mormont, Christian ULg

in Bulletin de Psychologie (1993), 47(415), 235-239

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See detailOptical imaging of the gravitational lens system B 1422+231
Remy; Surdej, Jean ULg; Smette, A. et al

in Astronomy and Astrophysics (1993), 278

Direct optical images (Bessel V, R and Gunn i; average seeing of 0.88 seconds) of B1422+231, obtained in March 1993 at European Southern Observatory (ESO) (La Silla), have been decomposed by fitting ... [more ▼]

Direct optical images (Bessel V, R and Gunn i; average seeing of 0.88 seconds) of B1422+231, obtained in March 1993 at European Southern Observatory (ESO) (La Silla), have been decomposed by fitting multiple Point Spread Functions (PSFs). We detect the optical counterparts of the four known radio point-like components. No additional optical image is found, but due to the compactness of the configuration, our detection limit for additional point sources is rather low (19.3 in R). The deduced positions and photometry of the four optical components agree pretty well with the radio and IR observations and this study provides further evidence supporting the gravitational lens origin of B1422+231. [less ▲]

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See detailTYPE OF WEAR FOR THE PAIR TI6A14V/PCTFE IN AMBIENT AIR AND IN LIQUID-NITROGEN
Bozet, Jean-Luc ULg

in Wear (1993), 162(Part B), 1025-1028

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V ... [more ▼]

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V/polychlorotrifluoroethylene (PCTFE) on a pin-on-disk tribometer in ambient air and liquid nitrogen with the contact pressure and sliding speed ranging, respectively, from 3 to 9 MPa and 0.03 to 0.05 m s-1. Because of the affinity of titanium for halogen elements, such as chlorine and fluorine, which are both present in PCTFE, we noticed the formation of an abrasive compound made of chlorine, fluorine, titanium and aluminium. These elements were detected by electron microscopy. In both environments the heat produced during sliding is responsible for the decomposition of the PCTFE. The surface of the polymer is softened by the heat and embedded abrasive particles then scratch the sliding track of the Ti6Al4V disk. This abrasiveness is confirmed with a three-dimensional stylus profilometer used on the Ti6Al4V disks. The low temperature and neutrality of liquid nitrogen hardly inhibit the decomposition of the polymer. This is related to the poor thermal conductivity of both titanium and PCTFE: the heat produced at the contact is fully available for the chemical reaction between the chlorine, fluorine and titanium, all the more so since this reaction requires little energy to take place. A better understanding of the wear behaviour of the pair PCTFE/Ti6Al4V in both air and liquid nitrogen should lead to the development of a surface treatment which prevents the formation of abrasive particles. We could then use titanium alloys as bulk materials for sliding parts in cryogenic valves and, because of the low specific mass of titanium, could benefit from the subsequent weight reduction. [less ▲]

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See detailAnalyse de la viande de poulet par spectroscopie infrarouge proche.
Sindic, Marianne ULg; Chevalier, Olivier; Dardenne, Pierre et al

in Viandes et Produits Carnés (1993), 14(4),

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See detailUtilisation d'un S.I.G. pour la gestion des ressources forestières : exemple d'application à un inventaire forestier régional
Lejeune, Philippe ULg; Lecomte, Hugues; Prevot, Hugues ULg

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(2-3), 275-286

The development of a Geographic Information System (G.I.S.) leads to new prospects in the field of broad-scale forest inventory, the main objective being to estimate areas and growing stock for the ... [more ▼]

The development of a Geographic Information System (G.I.S.) leads to new prospects in the field of broad-scale forest inventory, the main objective being to estimate areas and growing stock for the different stand types in a given region. The dot grid method typically used to estimate the areas in the case of systematic sampling, can be advantageously replaced by the creation within a G.I.S., of a stand map, that can be overlaid with data issued from the inventory. A methodology is proposed, to produce such a map using color infrared aerial photos at a scale of 1:25,000. Geographical data are processed using ILWIS software. The method has been tested on a 200 km² forest area. The error rate for interpretation of stand types is less than 6 % if one considers the confusions between species. The crossing of this stand map with the sample plots layer provides an estimation of the growing stock per stand type. [less ▲]

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See detailTuméfaction de la jambe à Neocosmospora vasinfecta chez un transplanté rénal. Premier cas d'infection humaine
Chandenier, Jacques; Hayette, Marie-Pierre ULg; de Bièvre, Claude et al

in Journal de Mycologie Médicale (1993), 3

A 46-year old man, originated from Senegal, but living in France for more than 15 years, is dialized since January 1989, because of renal insuficiency. He had undergone a kidney graft in October 1990 with ... [more ▼]

A 46-year old man, originated from Senegal, but living in France for more than 15 years, is dialized since January 1989, because of renal insuficiency. He had undergone a kidney graft in October 1990 with good initial evolution, without any graft rejection. The immunosuppressive tretment quite moderate, was prenisone 10mg/d, azathioprine 50 mg/d and ciclosporine 400mg/d. On the 8th month, a tumefaction affecting the anterior side of the left leg was discovered, which was thick, renitent and non inflammatory. It relapsed after two punctures. In both cases direct examination showed numerous hyphae and a fungus identified as Neocosmospora vasinfecta, was isolated by culture:it is the sexual form of an Ascomycete whose species name is not identified. The treatment associated a complete surgery and ketoconazole during a week (200mg/d) The tumor did not relapsed after a 30 months follow up. [less ▲]

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See detailEscherichia coli productrices de la toxine cytotoxique nécrosante de type 1 (CNF1) isolées à partir de processus pathologiques chez des chats et des chiens
Pohl, P.; Mainil, Jacques ULg; Devriese, L. et al

in Annales de Médecine Vétérinaire (1993), 137

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See detailReplacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme
Mainfroid, Véronique; Goraj, Karine; Rentier-Delrue, Françoise ULg et al

in Protein Engineering (1993), 6(8), 893-900

In order to investigate how structural modifications interfere with protein stability, we modified a (beta alpha)-unit in E.coli triosephosphate isomerase (TIM), a typical (beta alpha)-barrel protein ... [more ▼]

In order to investigate how structural modifications interfere with protein stability, we modified a (beta alpha)-unit in E.coli triosephosphate isomerase (TIM), a typical (beta alpha)-barrel protein, assuming that the pseudosymmetrical beta-barrel can be divided into eight successive loop/beta-strand/loop/alpha-helix motifs. We replaced the eighth (beta alpha)-unit of E.coli TIM with the corresponding chicken (beta alpha)-unit. The substitution, involving the replacement of 10 of the 23 residues of this (beta alpha)-unit, was evaluated first by modelling, then experimentally. Modelling by homology suggests how the amino acid replacements might be accommodated in the hybrid E.coli/chicken TIM (ETIM8CHI). Both natural and hybrid recombinant TIMs, overproduced in E.coli, were purified to homogeneity and characterized as to their stability and kinetics. Our kinetic studies show that the modification performed here leads to an active enzyme. The stability studies indicate that the stability of ETIM8CHI is comparable to that of the wild type TIM. [less ▲]

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See detailMédiastinites: diagnostic, facteurs de risque et traitement
Medot, M.; Fissette, J.; Limet, Raymond ULg

in Annales de Chirurgie (1993), 47(8), 752-65

The authors review the literature concerning post-sternotomy mediastinitis. The incidence, mortality, diagnosis and risk factors are discussed, but above all, the various treatments are reviewed and ... [more ▼]

The authors review the literature concerning post-sternotomy mediastinitis. The incidence, mortality, diagnosis and risk factors are discussed, but above all, the various treatments are reviewed and compared with respect to their advantages and risks, their respective usefulness and results, in order to define a reasonable way of management of this highly pejorative surgical complication. Open technique, closed irrigation and surgical flaps of omentum, pectoralis major and rectus abdominis are successively considered. [less ▲]

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See detailMolecular Biology of tilapia prolactins
Swennen, E.; Sekkali, B.; Poncelet, A. C. et al

in Fundamental and applied research for aquaculture (1993)

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See detailDémocratie, discussion et écriture chez Habermas et Lyotard
Delruelle, Edouard ULg

in Cahiers Internationaux de Symbolisme (1993), 74-76

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See detailAnalyse des comportements d'entraîneurs de volley-ball pendant des rencontres officielles
Cloes, Marc ULg; Delhaes, Jean-Pierre; Piéron, Maurice

in Sport (1993), 141(1), 16-25

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se ... [more ▼]

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se regrouper selon leurs objectifs: (1) l'analyse descriptive des comportements d'entraîneurs et de sportifs, parfois de très haut niveau; (2) la comparaison de situations pédagogiques, notamment en observant une même personne exerçant les fonctions d'entraîner et d'enseignant; (3) la comparaison des interventions des entraîneurs en fonction du niveau d'habileté des sportifs qu'ils dirigent; (4) la comparaison du comportement des entraîneurs en fonction des succès ou des échecs de leur équipe. ... [less ▲]

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