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See detailPlasma myeloperoxidase and vitamin E levels in head injury: preliminary results related to outcome.
Hans, Pol ULg; Franssen, Colette ULg; Pincemail, Joël ULg et al

in Journal of Neurosurgical Anesthesiology (1992), 4(1), 26-30

This preliminary study was designed to assess a possible role of neutrophil activation and to determine the prognostic value of plasma myeloperoxidase (MPO) and vitamin E (Vit. E) levels in severe head ... [more ▼]

This preliminary study was designed to assess a possible role of neutrophil activation and to determine the prognostic value of plasma myeloperoxidase (MPO) and vitamin E (Vit. E) levels in severe head injury. Plasma MPO and Vit. E levels were measured in nine severely head-injured patients (Glasgow Coma Score </=8) (ages 12-80 years) 6, 12. 18, 24, and 30 h after trauma. Patients were classified into two groups according to outcome after discharge from the ICU: group D (death; n = 5) and group S (survival; n = 4). Plasma MPO levels were increased immediately after trauma and then decreased. The MPO peak observed after 6 h was significantly higher (p < 0.05) in group D (mean +/- SEM: 1,237 +/- 122 ng/ml) than in group S (mean +/- SEM: 543 +/- 148 ng/ml). Plasma Vit. E levels were lower than normal values and decreased over time. They were always significantly lower (p < 0.05) in group D than in group S, except for the first sample. These differences cannot be explained entirely by total plasma lipid (TL) values since no statistical difference in TL concentrations was found between the two groups during the course of study. The ratio of Vit. E to TL. considered as the best index of Vit. E status, was lower in group D than in group S. and the difference reached statistical significance (p < 0.05) 12 h after trauma. In conclusion, in spite of the limited number of patients included in this study, it appears that severe head injury is associated with an increase in MPO and a decrease in Vit. E levels. These biochemical changes are of greater magnitude in group D than in group S; they suggest neutrophil activation and lipoperoxidation processes. Finally, plasma MPO and Vit. E seem to be new discriminant factors of outcome in head-injured patients. [less ▲]

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See detailLes caractères de l'amitié : Marché du livre et idéologie à Liège vers 1760
Droixhe, Daniel ULg

in Bulletin de la Société Royale Le Vieux-Liège (1992), 256-257

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See detailSubcutaneous erythropoietin for treatment of refractory anemia in hematologic disorders. Results of a phase I/II clinical trial.
Cazzola, M.; Ponchio, L.; Beguin, Yves ULg et al

in Blood (1992), 79(1), 29-37

We have used recombinant human erythropoietin (rHuEPO) in a phase I/II clinical trial to evaluate its ability to reverse refractory anemia in hematologic disorders. rHuEPO was administered subcutaneously ... [more ▼]

We have used recombinant human erythropoietin (rHuEPO) in a phase I/II clinical trial to evaluate its ability to reverse refractory anemia in hematologic disorders. rHuEPO was administered subcutaneously 5 days per week at escalating doses (50 to 150 U/kg per day). The aim of treatment was a hemoglobin (Hb) level greater than or equal to 10 g/dL without blood transfusion. Of 25 patients treated, 17 were evaluable, most of them with a regular need for transfusion. Eight of these had lymphoproliferative disorders (three cases of malignant lymphoma and five of monoclonal gammopathy) and were exposed to cytotoxic therapy. The other nine patients had hematopoietic stem cell disorders (four cases of myelodysplastic syndrome, three of idiopathic myelofibrosis, and two of chronic myelogenous leukemia). All patients with lymphoproliferative disorder had serum EPO levels inappropriately low for the degree of anemia, while patients with stem cell disorder showed variable values. Erythroid marrow activity was inadequate in all cases. Seven of eight patients with lymphoproliferative disorder responded to treatment maintaining Hb above 10 g/dL without transfusion. The median dose of rHuEPO required for correction of anemia was 75 U/kg. In four cases response was maintained with 50 U/kg, three times per week. There was no complete response among patients with hematopoietic stem cell disorder, although transfusion requirement was eliminated or reduced in four cases. Four patients developed functional iron deficiency during rHuEPO treatment and required iron supplementation to obtain response. Aggravation of splenomegaly was observed in two cases of myeloproliferative disorder. We conclude that: (1) subcutaneous administration of rHuEPO can be effective and safe in patients with lymphoproliferative disorder exposed to chemotherapy and showing inappropriate EPO response to anemia; (2) this is less likely in hematopoietic stem cell disorders, although favorable responses may be observed in occasional patients; and (3) functional iron deficiency as a cause of nonresponse to rHuEPO is frequent also in nonrenal anemia. [less ▲]

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See detailLutte contre la rage en Belgique: bilan épidémiologique 1991 et stratégie future
Coppens, P.; Brochier, Bernard; Costy, F. et al

in Annales de Médecine Vétérinaire (1992), 136

Two fox vaccination campaigns against rabies were carried out during spring and autumn 1991 in the Belgian contaminated area (10,000 km²). Vaccine-baits containing the vaccinia-rabies recombinant virus ... [more ▼]

Two fox vaccination campaigns against rabies were carried out during spring and autumn 1991 in the Belgian contaminated area (10,000 km²). Vaccine-baits containing the vaccinia-rabies recombinant virus were dropped by air at a mean density of 15 baits/km². In 1991, a drastic decrease in animal rabies cases was observed, as only 29 cases were reported in Belgium. The disease has disappeared from the largest part of the initially contaminated area and, since the beggining of the 2nd trimester 1991, all fox cases were reported exclusively along the French border. If the current epidemiological situation remains the same, the vaccination strategy for 1992 need to be geographically redefined; an immune belt 30 km wide should be created along our borders with France, Grand-Duchy of Luxembourg and Germany. [less ▲]

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See detailDe la stabilité d'un écoulement uniforme côtier dans un modèle à gravité réduite
Beckers, Jean-Marie ULg

in Comptes Rendus de l'Académie des Sciences. Série II, Mécanique, Physique, Chimie, Sciences de l'Univers, Sciences de la Terre (1992), 315(Série II), 783-789

The stability of uniform coastal current is analyzed in the framework of a reduced gravity model. The general solution of the eigenfunction problem is presented. Using an integral method, the stability of ... [more ▼]

The stability of uniform coastal current is analyzed in the framework of a reduced gravity model. The general solution of the eigenfunction problem is presented. Using an integral method, the stability of the flow to small pertubations is demonstrated for both exponential and resonant perturbations. [less ▲]

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See detailExamens electrophysiologiques dans la migraine.
Schoenen, Jean ULg

in Pathologie Biologie (1992), 40(4), 293-304

This article is a critical review of standard electroencephalography (EEG), brain mapping, evoked potential, and electromyography (EMG) studies carried out in various forms of migraine during and between ... [more ▼]

This article is a critical review of standard electroencephalography (EEG), brain mapping, evoked potential, and electromyography (EMG) studies carried out in various forms of migraine during and between attacks. With the exception of contingent negative variation, the exteroceptive silent period of the temporalis muscle, and possibly fast activity in response to visual stimuli, which are useful for the differential diagnosis of functional headache, the main value of electrophysiologic studies is to provide insight into the pathophysiology of the disease. Results of the different methods consistently indicate that the brain of migraine patients is characterized by hypersensitivity to some forms of stimuli between attacks and by, often focal, hypoactivity during attacks. [less ▲]

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See detailBâtons percés paléolithiques: décor figuratif, schématique et géométrique
Noiret, Pierre ULg

in Bulletin de l'Association scientifique liégeoise pour la Recherche archéologique (1992), XX

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See detailRaisonnements naturels en hydrodynamique
Blondin, Christiane ULg; Lafontaine, Dominique ULg; Closset, Jean-Louis ULg

in Revue Française de Pédagogie (1992), 100

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See detailNew data concerning the functional organization of the mammalian cell nucleolus: detection of RNA and rRNA by in situ molecular immunocytochemistry.
Thiry, Marc ULg

in Nucleic Acids Research (1992), 20(23), 6195-200

We have investigated the fine spatial distribution of RNA and rRNA within the Ehrlich tumor cell nucleolus by in situ hybridization with a biotin-labeled probe and by two new strategies, the polyadenylate ... [more ▼]

We have investigated the fine spatial distribution of RNA and rRNA within the Ehrlich tumor cell nucleolus by in situ hybridization with a biotin-labeled probe and by two new strategies, the polyadenylate nucleotidyl transferase-immunogold technique and immuno-labeling with anti-RNA antibodies. Besides the presence, as expected, of RNA and rRNA in the granular component and the dense fibrillar component, we show, for the first time, significant label over all the fibrillar centers of the nucleoli. When RNA and DNA were detected simultaneously on the same sections, only the fibrillar centers were positive for both. These results throw light on the controversial subject of the precise location of transcribing rRNA genes within the nucleolus. The fibrillar centers, and not the dense fibrillar component, should thus be the site of rRNA synthesis. [less ▲]

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See detailIntroductions et Réintroductions de mammifères en Belgique: Bilan et réflexions
Libois, Roland ULg

in Annales biologiques du Centre (1992), 4

During the last century, five mammal species were introduced into Belgium. One of them, the wild sheep, was introduced by hunters. Illicite releases are responsable of the presence of the coypu in many ... [more ▼]

During the last century, five mammal species were introduced into Belgium. One of them, the wild sheep, was introduced by hunters. Illicite releases are responsable of the presence of the coypu in many localities and the Siberian chipmunk in the vicinity of Brussels. The muskrat, widespread all over the country, the raccoon and some coypus are invaders from rearing facilities. Population reinforcements of game species (brown hare, red deer, wild boar...) are still frequent and are made without any control measures. Sometimes, hybrids between domestic pigs and wild boars are released in the wild by hunters. No project is officially supported to reintroduce extinct species. In some conservationist groups, however, there is some concern in doing something in favor of the otter and the beaver. [less ▲]

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See detailEchelles d'auto-évaluation de la dépression et de l'anxiété
Crasson, Marion ULg

in Psychologie Médicale (1992), 24(11), 1216-1220

Différents types d'échelle d'évaluation se sont développées depuis l'avènement des psychotropes dans les années 50. La passation de certaines d'entre elles, particulièrement les échelles d'auto-évaluation ... [more ▼]

Différents types d'échelle d'évaluation se sont développées depuis l'avènement des psychotropes dans les années 50. La passation de certaines d'entre elles, particulièrement les échelles d'auto-évaluation, peut se réaliser facilement via des systèmes informatisés. Nous présenterons deux de ces échelles, pour lesquelles nous disposons de données normatives et qui sont fréquemment utilisées dans notre laboratoire: l'échelle de dépression de Plutchik et van Praag (PVP) et l'échelle d'anxiété Etat-Trait de Spielberger et coll. (STAI). [less ▲]

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See detailThe Biology of Bovine Herpesvirus-4
Thiry, Etienne ULg; Bublot, M.; Dubuisson, J. et al

in Annales de Médecine Vétérinaire (1992), 136

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See detailExpression of members of the putative olfactory receptor gene family in mammalian germ cells.
Parmentier, M.; Libert, F.; Schurmans, Stéphane ULg et al

in Nature (1992), 355

A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the ... [more ▼]

A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the unidentified receptors ('orphan receptors'), a human genomic clone (HGMP07) was characterized by the presence of its transcripts in the testis and by its belonging to a large subfamily of genes sharing extensive sequence similarities. Sequence comparison demonstrated that this gene subfamily is the human counterpart of the putative rat olfactory receptors cloned recently. Another 48 members of the family were cloned. Northern blotting further demonstrated the presence of olfactory receptor transcripts in germ cells. Our finding suggests that a common receptor gene family encodes olfactory receptors and sperm cell receptors that could be involved in chemotaxis during fertilization [less ▲]

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See detailCongenital sebaceous trichofolliculoma
Kharfi, M.; Fazaa, B.; Mokhtar, I. et al

in Giornale Internazionale Dermatologia Pediatrica (1992), 4(1), 33-35

We report the clinical and histological presentation of a sebaceous trichofolliculoma present on the dorsum of the nose of a 1-year-old boy. This lesion was present since birth and appears to be the forst ... [more ▼]

We report the clinical and histological presentation of a sebaceous trichofolliculoma present on the dorsum of the nose of a 1-year-old boy. This lesion was present since birth and appears to be the forst reported cas of a congenital sebaceous trichofolliculoma. [less ▲]

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See detailSudoite in some Visean (Lower Carboniferous) K-bentonites from Belgium
Anceau, Annick ULg

in Clay Minerals (1992), 27

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See detailELISA test, a new method to detect and quantify isoproturon in soil.
Liegeois, Eric; Dehon, Yves; De Brabant, Bernard et al

in Science of the Total Environment (1992), 123/124

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See detailGenes involved in tumor invasion and metastasis are differentially modulated by estradiol and progestin in human breast-cancer cells.
van den Brule, F. A.; Engel, J.; Stetler-Stevenson, W. G. et al

in International Journal of Cancer = Journal International du Cancer (1992), 52(4), 653-7

Invasion of basement membranes by cancer cells is a critical step in metastasis, which requires the coordinated expression of specific genes such as laminin receptors and metalloproteinases. Estradiol and ... [more ▼]

Invasion of basement membranes by cancer cells is a critical step in metastasis, which requires the coordinated expression of specific genes such as laminin receptors and metalloproteinases. Estradiol and progesterone modulate the clinical progression of steroid-sensitive breast cancers; however, little is known about the molecular regulation of the invasive phenotype by these hormones. We therefore examined the effects of 10 nM estradiol and/or 10 nM progestin R5020 on the expression of 2 non-integrin laminin binding proteins, the 67-kDa laminin receptor (67LR) and HLBP31 as well as the 72-kDa type-IV collagenase (MMP-2) and its inhibitor, TIMP-2, in steroid-receptor-positive (T47D and MCF-7) and -negative (MDA-MB 231) human breast-cancer cells. The relative steady-state level of 67LR mRNA was increased 2- to 3-fold by estradiol in both MCF-7 (p < 0.001) and T47D (p < 0.001) cells, also by R5020, alone or in combination with estradiol, in T47D cells (p < 0.001) and to a much less extent in MCF-7 cells. HLBP31 mRNA and protein levels were increased 2- to 3-fold (p < 0.001) by R5020 alone or in combination with estradiol, but not by estradiol alone. None of the steroid treatments affected the expression or activity of MMP-2. Interestingly, however, TIMP-2 mRNA levels and protein expression in MCF-7 and T47D cells were 50% down-regulated (p < 0.001) by treatment with R5020 or R5020 plus estradiol, but not by treatment with estradiol alone. None of these genes were modulated in steroid-independent MDA-MB231 cells. The data suggest that estradiol and progesterone might act as coordinators regulating specific genes in the steroid-sensitive breast-cancer cell, leading to the acquisition of the metastatic phenotype. [less ▲]

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See detailLe secret médical dans la législation sociale
Kefer, Fabienne ULg

in Revue de Droit Pénal et de Criminologie (1992)

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See detailInfluence of laminin or fibroblasts upon colony formation in the mouse by B16 melanoma cell spheroids: a morphometric analysis.
Coucke, P.; de Leval, Laurence ULg; Leyh, P. et al

in In Vivo (Athens, Greece) (1992), 6(2), 119-24

By microscopical observation and using an original morphometric method, we analyzed on histological sections the rate of lung colony formation after the intravenous injection into the mouse of B16 ... [more ▼]

By microscopical observation and using an original morphometric method, we analyzed on histological sections the rate of lung colony formation after the intravenous injection into the mouse of B16 melanoma cells previously cultivated in vitro as aggregates. After the injection of B16 pure spheroids, superficial lung colonies were more numerous than internal lung colonies. After the injection of mixed spheroids (B16 + 3T3 fibroblasts), the size of colony sections was increased. Addition of laminin to pure or mixed spheroids decreased the size of colony sections but increased the number of internal lung colonies. [less ▲]

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See detailCaractérisation du sol vis-à-vis de l'assainissement.
Debbaut, Vincent ULg

in Tribune de l'Eau (La) (1992), 45(560), 57-66

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See detailEvidence for a relationship between Ehlers-Danlos type VII C in humans and bovine dermatosparaxis.
Nusgens, Betty ULg; Verellen-Dumoulin, C.; Hermanns-Le, Trinh ULg et al

in Nature Genetics (1992), 1(3), 214-7

Ehlers-Danlos (ED) syndrome type VII is characterized by the accumulation of collagen precursors in connective tissues. ED VII A and B are caused by mutations in the genes of alpha 1 and alpha 2 collagen ... [more ▼]

Ehlers-Danlos (ED) syndrome type VII is characterized by the accumulation of collagen precursors in connective tissues. ED VII A and B are caused by mutations in the genes of alpha 1 and alpha 2 collagen I which result in the disruption of the cleavage site of procollagen I N-proteinase. The existence of ED VII C in humans has been hypothesized on the basis of a disorder in cattle and sheep related to the absence of the enzyme. We now present evidence for the existence of this disease in humans, characterized by skin fragility, altered polymers seen as hieroglyphic pictures with electron microscopy, accumulation of p-N-alpha 1 and p-N-alpha 2 collagen type I in the dermis and absence of processing of the p-N-I polypeptides in fibroblast cultures. [less ▲]

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See detailUn nouveau timbre amphorique rhodien
Winand, Jean ULg

in Akkadica (1992), 79-80

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See detailThe 19-27 Amino-Acid Segment Of Gp51 Adopts An Amphiphilic Structure And Plays A Key Role In The Fusion Events Induced By Bovine Leukemia-Virus
Voneche, V.; Callebaut, I.; Kettmann, Richard ULg et al

in Journal of Biological Chemistry (1992), 267(21),

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S ... [more ▼]

Previous results indicate that the external glycoprotein gp51 of bovine leukemia virus plays an important role in the process of cell fusion induced by bovine leukemia virus (Bruck, C., Mathot, S., Portetelle, D., Berte, C., Franssen, J. D., Herion, P., and Burny, A. (1982) Virology 122, 342-352; Voneche, V., Portetelle., D., Kettmann, R., Willems, L., Limbach, K., Paoletti, E., Ruysschaert, J. M., Burny, A., and Brasseur, R. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 3810-3814) and suggest that a region encompassing residues 23 and 25 of gp51 is involved in this process (Portetelle, D., Couez, D., Bruck, C., Kettmann, R., Mammerickx, M., Van der Maaten, M., Brasseur, R., and Burny, A. (1989) Virology 169, 27-33; Mamoun, R., Morisson, M., Rebeyrotte, N., Busetta, B., Couez, D., Kettmann, R., Hospital, M., and Guillemain, B. (1990) J. Virol. 64, 4180-4188). X-ray diffraction studies performed on envelope glycoproteins of influenza virus indicate that the NH2-terminal part of the external glycoprotein lies very close to the fusion peptide. The same overall structure seems to exist in human immunodeficiency virus as suggested by site-directed mutagenesis followed by syncytia induction assays. Our theoretical studies indicate that a segment expanding between residues 19 and 27 of gp51 probably adopts an amphipathic beta-strand structure. We hypothesize that the amphipathic 19-27 structure of gp51 plays an important role in the process of membrane fusion by interacting with the fusion peptide or with another region of gp30. Mutational analysis disrupting the amphipathy of the 19-27 region strongly altered the fusogenic capacity of the gp51-gp30 complex. [less ▲]

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See detailQUENCHING OF A ROOM-TEMPERATURE FLUORESCENCE BAND AT 693-NM DURING PHOTOACTIVATION OF THE WATER-SPLITTING SYSTEM OF PHOTOSYSTEM-II IN FLASHED BARLEY LEAVES
Franck, Fabrice ULg; Dujardin, E.

in Photosynthesis Research (1992), 31(1), 41-47

The modifications of the room temperature fluorescence spectrum during the photoactivation of the water-splitting system by continuous illumination were investigated in flashed barley leaves. A blue shift ... [more ▼]

The modifications of the room temperature fluorescence spectrum during the photoactivation of the water-splitting system by continuous illumination were investigated in flashed barley leaves. A blue shift of the chlorophyll fluorescence band was detected during the first 2 min of illumination. During this shift, a decrease of the fluorescence intensity around 693 nm could be demonstrated in difference spectra and in second derivative spectra. This decrease is interpreted as a quenching of PS II fluorescence during the photoactivation. A relative fluorescence increase around 672 nm also occurred during the same period and is thought to reflect rapid light-induced chlorophyll formation. The flashed leaves contained small amounts of photoactive photochlorophyllide which could be removed by a short flash of intense white light given before continuous illumination. The fact that such flash had only weak effect on the 693 nm fluorescence decrease, whereas it strongly reduced the amplitude of the 672 nm fluorescence increase, favours the above interpretations. [less ▲]

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See detailMéthodologie de réalisation du schéma de structure communal en Région wallonne : Comptes rendus d'expériences, les communes de Fléron et de Dison
Collette, Jean-Pierre ULg; Goossens, Marc ULg; Hanocq, Philippe ULg

in Environnement et Société (1992), 9/10

Présentation des démarches qui ont été suivies pour réaliser les schémas de structure des communes de Fléron et de Dison et réflexions générales suscitées par ces deux expériences.

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See detailImmunomodulatory properties of cyclic hexapeptide oxytocin antagonists
Geenen, Vincent ULg; Martens, Henri ULg; Robert, Françoise et al

in Thymus (1992), 20

A pharmacological manipulation of cryptocrine cell-to-cell signaling was tested by the investigation of the immunomodulatory properties of novel cyclic hexapeptide oxytocin (OT) antagonists (MSD Research ... [more ▼]

A pharmacological manipulation of cryptocrine cell-to-cell signaling was tested by the investigation of the immunomodulatory properties of novel cyclic hexapeptide oxytocin (OT) antagonists (MSD Research Laboratories). The compounds were found to significantly inhibit the productions of IL-1ß and IL-6 elicited by anti-CD3 treatment oh human whole blood cells cultures (WBC). Cytokine productions were more significantly reduced by OT antagonists in WBC derived from female volunteers than in those obtained from male donors, suggesting an influence of sex steroids on the expression of NHP receptors by immune cells. These observations support the concept of novel immunomodulating approaches through immune-specific neuropeptide antagonists, as well as the pharmacological value of such strategies in selective immunotherapy. [less ▲]

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See detailVulvar basal cell carcinoma
Kharfi, M.; Mokhtar, I.; Fazaa, B. et al

in European Journal of Dermatology (1992), 2

Vulva is an uncommon localization for basal cell carcinomas. We report three cases and review the literature. There is often a long delay before patients seek medical attention for these lesions. Moreover ... [more ▼]

Vulva is an uncommon localization for basal cell carcinomas. We report three cases and review the literature. There is often a long delay before patients seek medical attention for these lesions. Moreover the clinical aspect is often indistinctive and misleading. The gross polymorphism of vulvar neoplasms therefore calls for biopsy of any suspicious areas. Surgical excision or Moh's chemotherapy are the treatments of choice for vulvar basal cell carcinomas. The recurrence rate remains quite high even when the lesion looks completely resected. [less ▲]

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See detailApplication de l'analyse CoMFA (Comparative Molecular Field Analysis) à une série de dérivés tricycliques apparentés à la clozapine
Liégeois, Jean-François ULg; Dupont, L.; Delarge, J.

in Journal de Pharmacie de Belgique (1992), 47

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See detailExpression of functional neurohypophysial peptide receptors by murine immature and cytotoxic T-cell lines
Martens, Henri ULg; Robert, Françoise; Geenen, Vincent ULg et al

in Progress in NeuroEndocrinImmunology (1992), 5

In order to demonstrate the cryptocrine model of neuroendocrine-immune interactions, specific neurohypophysial (NHP) receptors were detected on murine immature pre-T and cytotoxic T cell lines. A computer ... [more ▼]

In order to demonstrate the cryptocrine model of neuroendocrine-immune interactions, specific neurohypophysial (NHP) receptors were detected on murine immature pre-T and cytotoxic T cell lines. A computer analysis (LIGAND) revealed the heterogeneity of the binding sites. The Kd of Highest affinity were equivalent in both cell lines (0.15 nM), but the capacity (Bmax) of both low and high affinity sites was higher in the immature thymic lymphoma-derived pre-T-cell line. The functionality of these receptors was evidenced by their transduction of NHP-related ligands as an increase in intracellular inositol phosphates. These transductory properties were inhibited by a V1 receptor antagonist in pre-T cells, and by an oxytocin (OT) receptor antagonist in cytotoxic T cells. This work supports the involvement of NHP-related ligands and receptors in a process of cryptocrine signaling in the thymus, where the peptide concentration and the receptor affinity are in appropriate concordance. [less ▲]

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See detailIconographies de saints mosans : Luxembourg, B. Nat. Incunable M 289
George, Philippe ULg

in Bulletin de la Société Royale Le Vieux-Liège (1992)

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See detailMri-Pathological Correlations in Acute Traumatic Central Cord Syndrome: Case Report
Martin, Didier ULg; Schoenen, Jean ULg; Lenelle, Jacques ULg et al

in Neuroradiology (1992), 34(4), 262-6

Ante- and post-mortem MRI and detailed pathological examination were performed in a patient with a typical acute traumatic central cord syndrome (ATCCS) after a minor hyperextension injury to the neck who ... [more ▼]

Ante- and post-mortem MRI and detailed pathological examination were performed in a patient with a typical acute traumatic central cord syndrome (ATCCS) after a minor hyperextension injury to the neck who died 60 h later from heart failure. T2-weighted MRI showed a central hyperintense area at C3-4. There were disc protrusions, but no vertebral fracture or displacement. Histopathology disclosed severe axonal swelling and oedema in the dorsolateral fasciculi and, to a lesser degree, in the dorsal columns. In addition, an area of recent necrosis was found in the right anterior horn at C4-5. These findings suggest that the pathological hallmark of typical ATCCS is mechanical axonal disruption at a segmental level, but that more severe trauma may be accompanied by tissue destruction. [less ▲]

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See detailL'explication en Ethologie : quelques essais de modélisation - L'exemple des poissons.
Poncin, Pascal ULg; Ruwet, J. C.

in Probio revue (1992), 10(1-2), 71-82

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See detailReversed-Phase Hptlc Densitometric Evaluation of Fraxin in Fraxinus Excelsior Leaves
Poukens-Renwart, P.; Tits, Monique ULg; Wauters, Jean-Noël ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1992), 10(10-12, Oct-Dec), 1089-91

The present work describes a rapid and selective assay for fraxin based on its characteristic fluorescent properties by densitometry on reversed-phase HPTLC plates. Two chromatographgic procedures ... [more ▼]

The present work describes a rapid and selective assay for fraxin based on its characteristic fluorescent properties by densitometry on reversed-phase HPTLC plates. Two chromatographgic procedures (Silicagel and RP 18) have been assessed and the results compared to those obtained by reversed-phase HPLC on ODS-silica. The amount of fraxin found in six commercial batches of Fraxinus excelsior leaves ( based on dry weight) ranged from 0.1 to 0.2%. [less ▲]

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See detailPlant greening - Biogenesis of photosynthetic apparatus in bean leaves irradiated shortly after germination
Schoefs, B.; Bertrand, M.; Franck, Fabrice ULg

in Photosynthetica (1992), 27(4), 497-504

Formation of the photosynthetic apparatus in dark-grown 2-day-old bean (Phaseolus vulgaris L.) leaves was studied. The photosystem 2 (PS 2) reaction centres started functioning 1 h after the beginning of ... [more ▼]

Formation of the photosynthetic apparatus in dark-grown 2-day-old bean (Phaseolus vulgaris L.) leaves was studied. The photosystem 2 (PS 2) reaction centres started functioning 1 h after the beginning of irradiation. Electron transport between the two photosystems started after 4 h of irradiation. The PS 2 units were able to transfer the excitation energy to each other after 10 h of greening. The photosynthetic activity appeared a long time before the typical 77 K fluorescence bands of green leaves appeared. [less ▲]

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See detailComparative study of typical neuroleptics, clozapine and newly synthesized clozapine-analogues: correlations between neurochemistry and behaviour.
Bruhwyler, J.; Liégeois, Jean-François ULg; Chleide, E. et al

in Behavioural pharmacology (1992), 3(6), 567-579

While neuroleptic therapy with classical compounds has frequently been associated with extrapyramidal side effects, clozapine has revealed an interesting antipsychotic profile without producing any ... [more ▼]

While neuroleptic therapy with classical compounds has frequently been associated with extrapyramidal side effects, clozapine has revealed an interesting antipsychotic profile without producing any clearcut motor side effects. However, some adverse reactions remained that stimulated the search for improved antipsychotic agents. The aim of this study was to characterize the behavioural and neurochemical profiles of typical neuroleptics (chlorpromazine, haloperidol), clozapine, and four newly synthesized clozapine-analogues. Affinity for dopaminergic (D1,D2), serotonergic (5-HT(2)) and cholinergic (muscarinic) receptors were measured and the ratios of these different binding affinities were determined and correlated with the behavioural effects of the drugs in a complex temporal regulation task in the dog. The four clozapine-analogues showed most of the behavioural characteristics previously described for neuroleptics and their neurochemical profile, particularly their 5-HT(2)/D2 pKi ratio, was compatible with an atypical antipsychotic effect. Among these drugs, JL5 and JL13 showed a high degree of similarity with clozapine. Like clozapine, they did not induce catalepsy and stereotypy/hyperkinesia. Moreover, other motor effects were also reduced (ataxia, akinesia, dystony). and tremor and sialorrhea were completely absent with these two molecules. [less ▲]

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See detailPsychisme et greffe cardiaque
Triffaux, Jean-Marc ULg; Bertrand, Jean ULg

in Psychologie Médicale (1992)

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See detailLocation of DNA within the nucleolus of rat oocytes during the early stages of follicular growth.
Thiry, Marc ULg; Goessens, Guy ULg

in International Journal of Developmental Biology (1992), 36(1), 139-42

We have investigated the DNA distribution within the rat oocyte nucleolus during the early stages of follicular growth by means of the in situ terminal deoxynucleotidyl transferase method. In the ... [more ▼]

We have investigated the DNA distribution within the rat oocyte nucleolus during the early stages of follicular growth by means of the in situ terminal deoxynucleotidyl transferase method. In the fibrillogranular nucleolus, label is visualized on small clumps of peri- and intranucleolar chromatin. Such labeled clumps are frequently observed inside the interstices surrounding the fibrillar centers. Label is also consistently found in the fibrillar centers whereas the dense fibrillar component and the granular component are devoid of gold particles. These results contradict earlier data but conform with other recent immunocytochemical observations, obtained in nucleoli of a variety of somatic cell types, concerning the correlation between structure and function in the nucleolus. [less ▲]

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See detailGrowth hormone and prolactin stimulate androgen receptor, insulin-like growth factor-I (IGF-I) and IGF-I receptor levels in the prostate of immature rats.
Reiter, E.; Bonnet, Pierre ULg; Sente, B. et al

in Molecular & Cellular Endocrinology (1992), 88(1-3), 77-87

In this study we investigated the involvement of several different pituitary hormones on rat prostate development. 22-day-old Wistar rats, hypophysectomized (hypox) at 19 days of age were supplemented ... [more ▼]

In this study we investigated the involvement of several different pituitary hormones on rat prostate development. 22-day-old Wistar rats, hypophysectomized (hypox) at 19 days of age were supplemented with highly purified human prolactin (hPRL), human luteinizing hormone (hLH), porcine follicle-stimulating hormone (pFSH), and bovine growth hormone (bGH) or with saline. Quantitative analysis of RNAs shows that treatment with either PRL or GH increases significantly steady-state mRNAs levels of the following genes in the prostate: androgen receptor (AR) (respectively 3.5- and 4.8-fold above hypox controls), IGF-I (5- and 2.7-fold), and IGF-I receptor (2.9- and 2.3-fold). LH and FSH, by contrast, have negative effects on these parameters. To test whether the enhancing effect of PRL and GH on AR-mRNA abundance was followed by increased content in the protein itself, binding assays were performed with the androgen agonist [3H]R1881 (131 and 153 fmol/mg protein while hypox controls contained 110 fmol/mg protein). In addition to the well-documented presence of prolactin receptors in prostatic tissues, we have further demonstrated, by means of nuclease S1 protection assays plus dot- and Northern-blot analyses, that a GH receptor mRNA is produced in the immature rat prostate. Moreover, we observed not only strong lactogenic but also purely somatogenic binding to be occurring in the immature prostates. Finally, we have studied IGF-I mRNA content in separated epithelial/stromal cell fractions and have concluded that IGF-I expression is principally located in the prostatic stroma. Taken together, these results suggest that PRL and GH are involved in regulating AR synthesis, at least partially by direct action on the organ. In this context IGF-I appears as a paracrine factor playing a role in epithelium/stroma interactions during prostatic development. [less ▲]

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See detailVaricella-zoster virus: an ultrastructural study of the assembly phases
Schoonbroodt, Sonia; Piette, Jacques ULg; Rentier, Bernard ULg

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailPhotoionization Mass Spectrometry of Kinetic Energy-selected ions. The Translational Energy Distribution of N+/N2 in the Inner-shell Ionization energy range.
Locht, Robert ULg; Denzer, W.; Rühl, E. et al

in Chemical Physics (1992), 160

An ion retarding potential difference (IRPD) method has been used to investigate the ion yield and the kinetic energy distributions of N+/N2 produced by photoionization mass spectrometry using synchrotron ... [more ▼]

An ion retarding potential difference (IRPD) method has been used to investigate the ion yield and the kinetic energy distributions of N+/N2 produced by photoionization mass spectrometry using synchrotron radiation. Photoion yield curves of energy selected N+ ions are deduced. Translational energy distributions of N+ at the energies of the N(1s)->pi*, N(1s)->(nl)1 and above the N(1s)-1 threshold are determined. Comparison is made with previous photoion-photoelectron coincidence work using time-of-flight (TOF) measurements. [less ▲]

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See detailExtracellular matrix receptors and mouse skin carcinogenesis: altered expression linked to appearance of early markers of tumor progression.
Tennenbaum, T.; Yuspa, S. H.; Grover, A. et al

in Cancer Research (1992), 52(10), 2966-76

Interaction of cells with the basement membrane is important for cell proliferation and differentiation. Disruption of the basement membrane is an early event during progression of benign tumors to cancer ... [more ▼]

Interaction of cells with the basement membrane is important for cell proliferation and differentiation. Disruption of the basement membrane is an early event during progression of benign tumors to cancer. Using the techniques of immunohistochemistry and immunofluorescence, we show that cell-matrix interactions via the cell surface integrin receptors alpha 3 beta 1, alpha 5 beta 1, alpha 6 beta 4, the Mr 67,000 laminin receptor (67LR) laminin-binding protein, and the secreted matrix protein laminin are strictly regulated during differentiation of mouse epidermis. While alpha 6 beta 4 and alpha 5 beta 1 are polarized to the basal surface of basal cells in contact with the basement membrane, alpha 3 beta 1 and the non-integrin 67LR are primarily detected in the cell periphery of suprabasal cells, where cell to cell contacts are found. Sequential changes in expression of matrix receptors occur following multistage carcinogenesis of mouse skin. In an analysis of benign and malignant skin tumors induced by chemical carcinogens or oncogene transduction, we found that alpha 3 beta 1 and alpha 5 beta 1 as well as the non-integrin 67LR are sequentially down-regulated in the progression from benign to malignant, while alpha 6 beta 4 is the predominant receptor expressed in the carcinomas. Tumor expression of alpha 6 beta 4 is not polarized and is dissociated from its colocalized normal partner bullous pemphigoid antigen, which remains restricted to the basement membrane. The changes in matrix receptors are linked to appearance of keratin 13 in suprabasal regions, but always in alpha 6 beta 4 negative cells. The predominance of alpha 6 beta 4 in the proliferating cells during progression is associated with decreased expression of keratin 13 in carcinomas. These results suggest that matrix interactions with its receptors are important determinants of ordered differentiation in normal skin and show characteristic alterations during carcinogenesis that parallel changes in differentiation of the tumors. [less ▲]

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See detailHuman placental growth hormone
Frankenne, Francis; Evain-Brion, Danielle; Alsat, Eliane et al

in Anales Espanoles de Pediatria (1992), 36(50), 77-80

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See detailAntibodies to varicella-zoster virus modulate antigen distribution but fail to induce viral persistence in vitro.
Sadzot-Delvaux, Catherine ULg; Marc, Philippe; Lebon, Linda et al

in Journal of Virology (1992), 66(12), 7499-504

Varicella-zoster virus (VZV) persists in human sensory ganglia. One of the hypotheses to explain the induction or the maintenance of VZV latency is that it could be promoted by the immune response itself ... [more ▼]

Varicella-zoster virus (VZV) persists in human sensory ganglia. One of the hypotheses to explain the induction or the maintenance of VZV latency is that it could be promoted by the immune response itself. It is known that in the case of viruses which bud off the infected cell membrane, virus-specific antibodies can induce antigenic modulation, i.e., spatial redistribution of viral antigens and modulation of their synthesis. To determine whether antigenic modulation occurs during VZV infection in vitro and could possibly be involved in viral persistence, we have grown infected cells in the presence of anti-VZV antibodies either transiently or permanently. The distribution of immune complexes and viral proteins was then analyzed. In transient immunomodulation experiments, the distribution of one or more viral antigens was modified not only in the cytoplasmic membranes but also in the cytoplasm and nucleoplasm of infected cells. When infected cells were kept permanently in the presence of antibodies, the same pattern of redistribution of immune complexes was observed and the localization of internal viral glycoproteins was significantly modified. However, antibodies did not prevent the lytic effect of infection; they altered neither the infectious virus yield nor the Western immunoblot pattern of viral proteins, suggesting that immunomodulation is not the primary effector of viral persistence. [less ▲]

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See detailEndotoxin-induced microvascular injury in isolated and perfused pig lungs
Urbain, B.; Gustin, Pascal ULg; Ansay, Michel ULg

in Veterinary Research Communications (1992), 16(6), 453-464

The lungs of 13 healthy Landrace piglets were isolated, perfused and maintained in an isogravimetric state under zone III conditions. By applying vascular occlusion methods, the total blood flow ... [more ▼]

The lungs of 13 healthy Landrace piglets were isolated, perfused and maintained in an isogravimetric state under zone III conditions. By applying vascular occlusion methods, the total blood flow resistance (Rt) was partitioned into four components: arterial (Ra), pre- (Ra') and post-capillary (Rv'), and venous (Rv). The capillary filtration coefficient (Kfc) was evaluated using a gravimetric technique. A bolus of 55 micrograms of Escherichia coli endotoxins (LPS) per 100 g of lung was injected into the arterial reservoir of eight lungs, followed by an infusion of LPS at a rate of 55 micrograms per 100 g of lung per hour for 180 min. A bolus of theophylline (85 mg per 100 g of lung weight) was injected into the arterial reservoir after the last determination of the Kfc value. All the parameters were evaluated again when the lungs reached a new steady state. Endotoxin induced a significant increase in Rt from 54.7 +/- 7.0 at zero time to 184.7 +/- 44.2 cmH2O min L-1 (100 g)-1 180 minutes later, which can be ascribed to the increase in Ra' and Rv'. These haemodynamic modifications were related to the increases in the arterial pressure and in the pressure at the distal end of the arterial segment and to the decreases in the pressure at the proximal end of the venous segment and in the blood flow. The capillary pressure and the lung weight remained unchanged. Endotoxin infusion induced an increase in the Kfc value from 0.208 +/- 0.011 (at t = 0) to 0.391 +/- 0.034 ml min-1 (cmH2O)-1 (100 g)-1 (at t = 180). Administration of theophylline significantly reduced Rt,Ra,Ra' and Rv' towards or under the baseline values and also induced a significant increase in the lung weight and in the Kfc value. It was concluded that the endotoxin-induced increase in the total blood flow resistance can be ascribed to a vasospasm occurring at the level of the pre- and post-capillary small vessels and that changes in the permeability of the endothelium greatly contribute to the development of the pulmonary oedema observed in endotoxaemic pigs [less ▲]

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See detailCorrespondence analysis for quantification in electron energy loss spectroscopy and imaging.
Gelsema, E. S.; Beckers, Albert ULg; Sorber, C. W. et al

in Methods of Information in Medicine (1992), 31(1), 29-35

Electron energy loss spectroscopy (EELS) is a technique to investigate the physical properties of material. Using this technique it is possible to detect the presence of a specific element in a specimen ... [more ▼]

Electron energy loss spectroscopy (EELS) is a technique to investigate the physical properties of material. Using this technique it is possible to detect the presence of a specific element in a specimen. When used in combination with an electron microscope, energy filtered images may be obtained, which in principle may be used to quantify the local element concentration. This involves a process of background correction, conventionally performed assuming a specific parametric behavior of the spectral intensity as a function of electron energy loss. In this article a parameter-free method is described for background correction based on the formalism of correspondence analysis. Such a method may be used in parts of the spectrum where the functional dependence of the spectral intensity is unknown. Use of this method for element detection has been suggested before. This article reports simulation experiments suggesting its suitability for quantitative determination of element distributions and element concentrations. [less ▲]

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See detailA quasi-Newton algorithm for first-odrer saddle-point location
Culot, Patrick; Dive, Georges ULg; Nguyen, Van Hen et al

in Theoretica Chimica Acta (1992), 82

A new algorithm for the location of a transition-state structure on an energy hypersurface is proposed. The method is compared to three other quasi-Newton step calculations available in literature ... [more ▼]

A new algorithm for the location of a transition-state structure on an energy hypersurface is proposed. The method is compared to three other quasi-Newton step calculations available in literature. Numerical results derived from several examples are compared to those obtained by the two algorithms implemented in the Gaussian package. [less ▲]

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See detailLes biopesticides en arboriculture fruitiere.
Lepoivre, Philippe ULg; Burgeff, C.; Jijakli, Mohamed ULg et al

in Fruit Belge (1992), 60(440), 242-248

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See detailTransition Rates in the 3d2 Configuration of the Calcium Isoelectronic Sequence
Biémont, Emile ULg; Hansen, J. E.; Quinet, Pascal ULg et al

in Journal of Physics : B Atomic Molecular & Optical Physics (1992), 25

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See detailLes champs électromagnétiques ELF ont-ils un effet sur la santé? Revue de la littérature
Crasson, Marion ULg; Timsit-Berthier, Martine; Legros, Jean-Jacques ULg

in Psychologie Médicale (1992), 24(11), 1205-1215

De nombreuses études ont été entreprises les dernières décénnies sur les effets des champs électromagnétiques de très basse fréquence (TBF ou ELF). Les rapports publiés sont tantôt optimistes tantôt ... [more ▼]

De nombreuses études ont été entreprises les dernières décénnies sur les effets des champs électromagnétiques de très basse fréquence (TBF ou ELF). Les rapports publiés sont tantôt optimistes tantôt pessimistes. Les auteurs passent en revue les recherches épidémiologiques et expérimentales effectuées chez l'homme avant d'envisager une hypothèse explicative des effets observés, en relation avec le rôle joué par la glande pinéale [less ▲]

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See detailAcyclovir vs isoprinosine (immunovir) for suppression of recurrent genital herpes simplex infection.
Kinghorn, G. R.; Woolley, P. D.; Thin, R. N. et al

in Genitourinary Medicine (1992), 68(5), 312-6

OBJECTIVE: To compare the efficacy and safety of oral acyclovir (400 mg twice daily) with oral isoprinosine (500 mg twice daily) in the suppression of recurrent genital herpes. DESIGN: Double-blind ... [more ▼]

OBJECTIVE: To compare the efficacy and safety of oral acyclovir (400 mg twice daily) with oral isoprinosine (500 mg twice daily) in the suppression of recurrent genital herpes. DESIGN: Double-blind, double-dummy, randomised, controlled, parallel group trial. SETTING: 13 centres in UK, Belgium and Germany. SUBJECTS: 127 immunocompetent patients with frequently recurring genital herpes. MAIN OUTCOME MEASURES: Proportions of patients reporting recurrences, recurrence frequency, and mean duration of lesions during breakthrough recurrences in each treatment group during a 6 month treatment period; time to first recurrence during treatment and follow-up after treatment cessation. RESULTS: During treatment, acyclovir recipients showed significant differences (p < 0.05) when compared with isoprinosine recipients in terms of a lower proportion reporting recurrences (31% vs 96%), a reduced mean number of reported recurrences per patient (0.6 vs 3.6), a shorter mean duration of breakthrough lesions (6.4 days vs 8.2 days), and a longer mean time (standard error) to first recurrence (143.7 (9.1) days vs 40.5 (5.4) days. The mean time to first recurrence after treatment cessation did not differ between the two groups. As compared with placebo recipients, isoprinosine treated patients had an increased recurrence frequency (3.6 vs 2.5) during treatment, and a shorter time to first recurrence after treatment cessation. All treatments were well tolerated without serious adverse events or toxicity. CONCLUSIONS: Acyclovir is very effective in suppressing recurrent genital herpes and is clearly superior to isoprinosine which is not clinically useful in the dosage studied. [less ▲]

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See detailAdhésines connues et inconnues chez les Escherichia coli isolées de cas de diarrhée du veau
Pohl, P.; Van Muylem, K.; Lintermans, P. et al

in Annales de Médecine Vétérinaire (1992), 136

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See detailFacilitation of Responses to Motor Cortex Stimulation: Effects of Isometric Voluntary Contraction
Maertens De Noordhout, Alain ULg; PEPIN, Jean-Louis ULg; GERARD, Pascale ULg et al

in Annals of Neurology (1992), 32(3), 365-70

In 7 normal subjects we compared the facilitatory effect of isometric contraction of the tibialis anterior on the size of electromyographic responses evoked in this muscle by electric stimuli applied over ... [more ▼]

In 7 normal subjects we compared the facilitatory effect of isometric contraction of the tibialis anterior on the size of electromyographic responses evoked in this muscle by electric stimuli applied over the cervical column and by electric and magnetic percutaneous stimulation of the motor cortex. No significant difference was found between the degrees of facilitation of the responses to any of the stimuli. Using collision techniques, we also showed that the pyramidal fibers activated by spinal and cortical stimuli are the same. Facilitation induced by isometric contraction (20% maximum) was of similar or greater magnitude than that found with constant vibration of the tendon of the target muscle. In cases where vibration and contraction had equal facilitatory effects, there was no further facilitation of the responses when both conditions were applied together. These findings indicate that the facilitatory effect of isometric contraction of the target muscle essentially originates at a spinal level rather than in the motor cortex. [less ▲]

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See detailUne question de génétique : wallon "tchawe-sori" et français "chauve-souris"
Boutier, Marie-Guy ULg

in Travaux de linguistique et de philologie (1992), 30

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See detailMort, mentalité et tradition à la lumière des ballades de Terre-Neuve
Peere, Isabelle ULg

in Canadian Folklore Canadien (1992), 14(2), 137-58

A talerole analysis of prominent Newfoundland ballad and narrative song records on the theme of death highlighting coherent meaning throughout the different layers of the local song tradition, i.e. an ... [more ▼]

A talerole analysis of prominent Newfoundland ballad and narrative song records on the theme of death highlighting coherent meaning throughout the different layers of the local song tradition, i.e. an exclusive and constant preoccupation with tragic, sudden, premature and violent death as an inspiration to courage and responsibility rather than despair or resignation. [less ▲]

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See detailLa matière en suspension de la couche de surface du plateau continental nord-ouest européen. I. Distribution spatiale de la biomasse et du rapport 13C/12C
Dauby, Patrick ULg; Mosora, Fiorentina; Frankignoulle, Michel et al

in Bulletin de la Société Royale des Sciences de Liège (1992), 61

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See detailNouvelles approches pharmacologiques basées sur les interactions neuroendocrino-immunitaires
Geenen, Vincent ULg; Franchimont, Nathalie; Louis, Edouard ULg et al

in Médecine et Hygiène (1992), 50

La description des interactions neuroendocrino-immunitaires a été un domaine de recherche très fructueux au cours des dernières années. Si les circonstances physiopathologiques au cours desquelles ces ... [more ▼]

La description des interactions neuroendocrino-immunitaires a été un domaine de recherche très fructueux au cours des dernières années. Si les circonstances physiopathologiques au cours desquelles ces interactions interviennent restent encore à préciser, la caractérisation biochimique de signaux neuropeptidiques et de leurs récepteus exprimés par des cellules immunocompétentes permet d'entrevoir de nouvelles possibilités thérapeutiques. En particulier, l'action immunomodulatrice et antiinflammatoire potentielle d'antagonistes spécifiques de neuropeptides représente une voie intéressante pour la mise au point d'immunothérapies sélectives. [less ▲]

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See detailCharacterization of regulatory functions of the varicella-zoster virus gene-63-encoded protein
Jackers, Pascale ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Journal of Virology (1992), 66(6), 3899-3903

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex ... [more ▼]

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex virus type 1. ICP22 is a polypeptide synthesized in herpes simplex virus type 1-infected cells, and as is the case for its VZV counterpart, its regulatory functions are unknown. On the basis of the VZV DNA sequence, it has been shown that IE63 exhibits hydrophilic and acidic properties, suggesting that this protein could play a regulatory role during the infectious cycle. We report in this article cotransfection experiments which demonstrate that the VZV gene 63 protein strongly represses, in a dose-dependent manner, the expression of VZV gene 62. On the other hand, transient expression of the VZV gene 63 protein can promote activation of the thymidine kinase gene but cannot affect the expression of the genes encoding glycoproteins I and II. The results of transient expression experiments strongly suggest that the VZV gene 63 protein could play a pivotal role in the repression of IE gene expression as well as in the activation of early gene expression [less ▲]

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See detailStudy of the antagonism developped by fluorescent Pseudomonas against Pythium spp. using Tn5 and NTG mutants.
Jacques, Ph.; Mistry, C.; Ongena, Marc ULg et al

in Archives Internationales de Physiologie et de Biochimie (1992), 101(B21),

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See detailThe effects of selection on linkage analysis for quantitative traits
Mackinnon, M. J.; Georges, Michel ULg

in Genetics (1992), 132

The effects of within-sample selection on the outcome of analyses detecting linkage between genetic markers and quantitative traits were studied. It was found that selection by truncation for the trait of ... [more ▼]

The effects of within-sample selection on the outcome of analyses detecting linkage between genetic markers and quantitative traits were studied. It was found that selection by truncation for the trait of interest significantly reduces the differences between marker genotype means thus reducing the power to detect linked quantitative trait loci (QTL). The size of this reduction is a function of proportion selected, the magnitude of the QTL effect, recombination rate between the marker locus and the QTL, and the allele frequency of the QTL. Proportion selected was the most influential of these factors on bias, e.g., for an allele substitution effect of one standard deviation unit, selecting the top 80%, 50% or 20% of the population required 2,6 or 24 times the number of progeny, respectively, to offset the loss of power caused by this selection. The effect on power was approximately linear with respect to the size of gene effect, almost invariant to recombination rate, and a complex function of QTL allele frequency. It was concluded that experimental samples from animal populations which have been subjected to even minor amounts of selection will be inefficient in yielding information on linkage between markers and loci influencing the quantitative trait under selection. [less ▲]

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See detailHistory and Dispossession in John Glenday's Poetry
Pagnoulle, Christine ULg

in Ecosse : Littérature et Civilisation (1992), 12

This short essay discusses Glenday's poetic exploration of history, especially the tragedy of dispossession during the enclosure movement.

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See detailStructure du 6-(4-méthylpipérazin-1-yl)-11H-pyrido[2,3-b][1,4]benzodiazépine 1,5-hydrate
Dupont, L.; Englebert, S.; Dideberg, O. et al

in Acta Crystallographica Section C-Crystal Structure Communications (1992), C48

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See detailColour rendering of supplementary artificial lighting
Embrechts, Jean-Jacques ULg

in Color Research and Application (1992), 17(3)

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See detailLes peintures de la cathédrale de Liège.Histoire & restauration
George, Philippe ULg; Lhoist-Colman Berthe

in Feuillets de la Cathédrale de Liège (1992)

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See detailL'enseignement technique agricole et le défi de l'environnement : passé et futur...
Mougenot, Catherine ULg

in Revue de l'Institut de Sociologie (1992), 1(4), 265-290

Une analyse de l’enseignement agricole technique reposant sur deux fils rouges : l’histoire de l’institution et la nécessité pour celle-ci de prendre en compte les nouvelles perspectives environnementales.

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See detailNouvelles méthodes de lutte
Schiffers, Bruno ULg; Copin, Alfred; Roland, Liliane

in Revue de l'Agriculture (1992), Hors série

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See detailLight and electron microscopic immunolocalization of bovine pregnancy-associated glycoprotein in the bovine placentome.
Zoli, A.; Demez, Pierre ULg; Beckers, Jean-François ULg et al

in Biology of Reproduction (1992), 46(4), 623-9

A bovine pregnancy-associated glycoprotein (bPAG) of 67 kDa has previously been isolated from bovine fetal cotyledons. The objective of this study was to determine the cytological localization of that ... [more ▼]

A bovine pregnancy-associated glycoprotein (bPAG) of 67 kDa has previously been isolated from bovine fetal cotyledons. The objective of this study was to determine the cytological localization of that protein in the placentomes and possibly the cells responsible for its production. Highly specific antisera raised against pure bPAG were used to demonstrate the cellular localization of the protein in bovine placentomes by light and electron microscopic techniques. Strong immunostaining was observed exclusively in the cytoplasm of large binucleate cells present predominantly in fetal cotyledonary tissue (villi). Some smaller weakly immunostained cells were also present in caruncular epithelium. By ultrastructural immunogold procedures, the protein was detected only within amorphous cytoplasmic granules. Granules of identical size, but weakly labeled, were found on the maternal side. All cells containing labeled granules were binucleate. These results suggest that bPAG is probably synthesized by trophoblast binucleate cells and stored in granules prior to delivery into the maternal circulation after cell migration. [less ▲]

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See detailThe candidate oncoprotein Bcl-3 is an antagonist of p50/NF-kappa B-mediated inhibition.
Franzoso, G.; Bours, Vincent ULg; Park, S. et al

in Nature (1992), 359(6393), 339-42

The candidate oncogene bcl-3 was discovered as a translocation into the immunoglobulin alpha-locus in some cases of B-cell chronic lymphocytic leukaemias. The protein Bcl-3 contains seven so-called ... [more ▼]

The candidate oncogene bcl-3 was discovered as a translocation into the immunoglobulin alpha-locus in some cases of B-cell chronic lymphocytic leukaemias. The protein Bcl-3 contains seven so-called ankyrin repeats. Similar repeat motifs are found in a number of diverse regulatory proteins but the motifs of Bcl-3 are most closely related to those found in I kappa B proteins in which the ankyrin repeat domain is thought to be directly involved in inhibition of NF-kappa B activity. No biological function has yet been described for Bcl-3, but it was noted recently that Bcl-3 interferes with DNA-binding of the p50 subunit of NF-kappa B in vitro. Here we demonstrate that Bcl-3 can aid kappa B site-dependent transcription in vivo by counteracting the inhibitory effects of p50/NF-kappa B homodimers. Bcl-3 may therefore aid activation of select NF-kappa B-regulated genes, including those of the human immunodeficiency virus. [less ▲]

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See detailEvaluatie van de nieuwe dopamine-agonist CV 205-502 in de behandeling van prolactinomen
Verhelst, J.; Abs, R.; Beckers, Albert ULg et al

in Acta Antwerpiensia (1992), 9

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See detailLe traitement des prolactinomes par un nouvel agoniste dopaminergique (le CV 205-502) : résultats chez 48 patients.
Beckers, Albert ULg; Ghuysen, A. E.; Abs, R. et al

in Médecine et Hygiène (1992), 50

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See detailEpreuves isocinétiques de fatigabilité musculaire
Croisier, Jean-Louis ULg; Lavis, C.; Crielaard, Jean-Michel ULg

in Actualités Sports et Médecine (1992), 20

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See detailRapport préliminaire sur la 1ère campagne de fouille à Tell Amarna (Syrie)
Tunca, Önhan ULg

in Akkadica (1992), 79-80

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See detailLes adénomes hypophysaires à TSH.
Beckers, Albert ULg; Stevenaert, Achille ULg

in Revue Française d'Endocrinologie Clinique, Nutrition, et Métabolisme (La) (1992), 33(2), 151-156

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See detailLocation and Characterization of the Bovine Herpesvirus Type 4 Thymidine Kinase Gene; Comparison with Thymidine Kinase Genes of Other Herpesviruses
Lomonte, P.; Bublot, M.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1992), 127(1-4), 327-37

The location and nucleotide sequence of the bovine herpesvirus type 4 (BHV-4) thymidine kinase (TK) gene was determined. The coding region of the TK gene is 1335 nucleotides long and corresponds to a ... [more ▼]

The location and nucleotide sequence of the bovine herpesvirus type 4 (BHV-4) thymidine kinase (TK) gene was determined. The coding region of the TK gene is 1335 nucleotides long and corresponds to a polypeptide of 445 amino acids. Comparison of TK amino acid sequences of BHV-4 and 16 herpesvirus TKs reveals a greater homology to those of the gammaherpesviruses EBV and specially HVS, than to those of alphaherpesviruses. The open reading frames detected in the vicinity of TK gene were homologous to the corresponding ones in other herpesviruses. [less ▲]

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See detailTwo-dimensional study of varicella-zoster virus proteins
Debrus, S.; Lebon, L.; Schoonbroodt, Sonia et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100(2), 39

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See detailMeasuring Technical Efficiency in European Railways: A Panel Data Approach
Gathon, Henry-Jean ULg; Perelman, Sergio ULg

in Journal of Productivity Analysis (1992), 3

We estimate a factor requirement frontier for European railways using a panel data approach in which technical efficiency is assumed to be endogencously determined. This approach has two main outcomes. On ... [more ▼]

We estimate a factor requirement frontier for European railways using a panel data approach in which technical efficiency is assumed to be endogencously determined. This approach has two main outcomes. On one hand, it allows the identification of factors influencing technical efficiency, and on the other hand, it allows the estimation of alternative efficiency indicators free of these influences. In the case under study, a particular attention is devoted to an autonomy indicator representing the managerial freedom, with respect to public authorities, experienced by firms, that appears to be positively correlated with technical efficiency. [less ▲]

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See detailA gel retardation assay system for studying protein binding to simple repetitive DNA sequences.
Maueler, W.; Muller, Marc ULg; Kohne, A. C. et al

in Electrophoresis (1992), 13(1-2), 7-10

Simple repetitive DNA sequences have been regarded as mere "junk" present in all eukaryotic genomes. In fact, mixed simple repeat (gt)n(ga)m sequences are present in major histocompatibility complex MHC ... [more ▼]

Simple repetitive DNA sequences have been regarded as mere "junk" present in all eukaryotic genomes. In fact, mixed simple repeat (gt)n(ga)m sequences are present in major histocompatibility complex MHC-DRB genes for long evolutionary times, including such distant animals as artiodactyla and man. We describe herein an unsophisticated method which reveals that at least certain simple repetitive (gt)n(ga)m sequences bind nuclear proteins and show characteristics of a specific DNA-protein interaction via gel retardation. [less ▲]

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See detailHIV-1 reactivation after an oxidative stress
Legrand, Sylvie ULg; Hoebeke, Maryse ULg; Vaira, Dolorès ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailDéveloppement d'un Gestionnaire Automatisé de Bibliographies selon les normes de l'American Psychological Association (GABI APA Style)
Thirion, Paul ULg

in Revue. Informatique et Statistique dans les Sciences Humaines (1992), XXVIII(1-4), 251-284

Nowadays, there are plenty of complete and user-friendly database management systems. However, most of them only produce poorly organized raw data, generally lacking of internal consistency. This ... [more ▼]

Nowadays, there are plenty of complete and user-friendly database management systems. However, most of them only produce poorly organized raw data, generally lacking of internal consistency. This situation remains awkward, especially for scientists who have regularly to report references in an extremely ruled international format. GABI APA Style was built in titis context. GABl APA Style is a reference manager software using the presentation rules of the American Psychological Association (A.P.A.) It was written in Clipper language conforming to the relational database principle. The initial aim was to elaborate a software to centralize references, filling in the intellectual background of the Department od Psychology and Educational Sciences of Liège University. Special attention was paid to the ergonomic and easy-to-use aspects of the software, just as the possibilities of transfers with other applications. [less ▲]

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See detailSYNTHESIS OF NONACTIVATED F-18 FLUORINATED AROMATIC-COMPOUNDS THROUGH NUCLEOPHILIC-SUBSTITUTION AND DECARBOXYLATION REACTIONS
Plenevaux, Alain ULg; Lemaire, Christian ULg; Palmer, Anthtony J. et al

in Applied Radiation & Isotopes (1992), 43(8), 1035-1040

The synthesis of no-carrier-added 3-[F-18]fluoroanisole, 2-[F-18]fluoroanisole, [F-18]fluorobenzene and 4-[F-18]fluoroveratrole are reported. The strategy consists of amino-polyether supported ... [more ▼]

The synthesis of no-carrier-added 3-[F-18]fluoroanisole, 2-[F-18]fluoroanisole, [F-18]fluorobenzene and 4-[F-18]fluoroveratrole are reported. The strategy consists of amino-polyether supported nucleophilic substitution with [F-18]F- on activated nitro aromatic aldehyde precursors followed by decarbonylation using Tris(triphenylphosphine) rhodium (I) chloride. The experimental parameters for this reaction have been studied and optimized with 2-[F-18]fluoro-4-methoxybenzaldehyde and then successfully applied to four other F-18-fluorinated aromatic aldehydes. The decarbonylation yields obtained were 84 +/- 5% (corrected for decay) within 15 min at 150-degrees-C in 1,4-dioxan. [less ▲]

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See detailMolecular modeling of the amphipathic helices of the plasma apolipoproteins.
Brasseur, Robert ULg; Lins, Laurence ULg; Vanloo, B. et al

in Proteins (1992), 13(3), 246-57

In this paper we propose a classification of the amphipathic helical repeats occurring in the plasma apolipoprotein sequences. It is based upon the calculation of the molecular hydrophobicity potential ... [more ▼]

In this paper we propose a classification of the amphipathic helical repeats occurring in the plasma apolipoprotein sequences. It is based upon the calculation of the molecular hydrophobicity potential around the helical segments. The repeats were identified using a new autocorrelation matrix, based upon similarities of hydrophobic and hydrophilic properties of the amino acid residues within the apolipoprotein sequences. The helices were constructed by molecular modeling, the molecular hydrophobicity potential was calculated, and isopotential contour lines drawn around the helices yielded a three-dimensional visualization of the hydrophobicity potential. Two classes of apolipoproteins could be differentiated by comparing the hydrophobic angles obtained by projection of the isopotential contour lines on a plane perpendicular to the long axis of the helix. The isopotential contour lines around apo AI, AIV, and E are more hydrophilic than hydrophobic, whereas they are of similar intensity for apo AII, CI, and CIII. In both cases discoidal lipid-protein complexes are generated, with the amphipathic helices around the edge of the lipid core. The long axis of the helices is oriented parallel to the phospholipid acyl chains and the hydrophilic side of the helix toward the aqueous phase. As a result of the differences in hydrophobicity potential, the contact between the hydrophobic side of the helices and the phospholipid acyl chains is larger for apo AII, CI, and CIII than for the other apolipoproteins. This might account for the greater stability of the discoidal complexes generated between phospholipids and these apoproteins. [less ▲]

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See detailDetermination of Meprobamate in Pharmaceutical Dosage Forms Also Containing Carbromal by Liquid Chromatography and Indirect Photometric Detection
Bechet, I.; Ceccato, Attilio ULg; Hubert, Philippe ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1992), 10(10-12, Oct-Dec), 995-9

In a pharmaceutical form also containing carbromal, meprobamate could not be quantified selectively by classical methods described in pharmacopoeias due to a significant interference from carbromal ... [more ▼]

In a pharmaceutical form also containing carbromal, meprobamate could not be quantified selectively by classical methods described in pharmacopoeias due to a significant interference from carbromal. Consequently, reversed-phase HPLC methods have been developed to separate the two active ingredients using indirect photometric detection to visualize and determine meprobamate which has very poor chromophoric properties. Different parameters influencing the sensitivity of the indirect response, such as the nature of the highly absorbing compound added to the mobile phase (the marker) as well as the methanol content and the pH of this phase, have been studied. Two chromatographic systems containing benzoic acid or cinnamic acid as the marker, have been optimized and validated. Good linearity and reproducibility have been obtained with both systems but the cinnamic acid method has the advantage that meprobamate and carbromal can be determined simultaneously at 273 nm. [less ▲]

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See detailRemotely sensed data contributes to GIS socioeconomic analysis
Donnay, Jean-Paul ULg

in GIS-Europe (1992), 1(10), 38-41

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See detailTuberculosis in dogs : a case report and review of the literature
Clercx, Cécile ULg; Coignoul, Freddy ULg; Jakovlevic, S. et al

in Journal of the American Animal Hospital Association (1992), 28

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See detailErythropoiesis in multiple myeloma: defective red cell production due to inappropriate erythropoietin production.
Beguin, Yves ULg; Yerna, M.; Loo, Martine et al

in British Journal of Haematology (1992), 82(4), 648-53

We investigated the pathophysiology of erythropoiesis in 62 patients with multiple myeloma and examined whether it would establish a rational basis for the treatment of their anaemia with recombinant ... [more ▼]

We investigated the pathophysiology of erythropoiesis in 62 patients with multiple myeloma and examined whether it would establish a rational basis for the treatment of their anaemia with recombinant human erythropoietin. Erythropoietin (Epo) production was evaluated by serum levels and erythropoiesis was quantitated by serum transferrin receptor (TfR) levels, both assessed relative to the degree of anaemia. Instead of the expected stimulation of erythropoiesis in response to anaemia, haematocrit correlated positively with marrow erythropoietic activity, indicating that the mechanism of anaemia was primarily defective red cell production. Erythropoiesis decreased and anaemia worsened significantly with advancing clinical stage. 25% of the patients had inadequate Epo production and this proportion increased to 50% in stage 3. Inappropriate Epo production was seen in 60% of patients with renal impairment but was also observed in a number of patients with normal renal function. Erythropoiesis correlated strongly with the adequacy of Epo production, particularly in advanced disease. We conclude that most myeloma patients have defective red cell production even in the absence of massive marrow infiltration and that inappropriate Epo production contributes to their anaemia. [less ▲]

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See detailBeta estimates of dutch firms : alternate methods
Corhay, Albert ULg; Tourani-Rad, Alireza

in Vba Journaal (1992), 8(4), 2-6

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See detailDirect stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation.
De Groote, D.; Zangerlé, Pierre-François ULg; Gevaert, Y. et al

in Cytokine (1992), 4(3), 239-48

Production of interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), interleukin 2 (IL-2), interferon gamma (IFN-gamma) and granulocyte-macrophage colony ... [more ▼]

Production of interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), interleukin 2 (IL-2), interferon gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF) after stimulation by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) was studied in 1/10 diluted whole blood (WB) culture and in peripheral blood mononuclear cell (PBMC) culture. Cytokines IL-1 beta, TNF-alpha and IL-6 are preferentially stimulated by LPS whereas IL-2, IFN-gamma and GM-CSF are stimulated by PHA. Combination of 5 micrograms/ml PHA and 25 micrograms/ml LPS gave the most reliable production of the six cytokines studied. IL-1 beta, TNF-alpha and IL-6 represent a homogeneous group of early-produced cytokines positively correlated among themselves and with the number of monocytes in the culture (LeuM3). Furthermore, IL-1 beta was negatively correlated with the number of T8 lymphocytes. IL-2, IFN-gamma and GM-CSF represent a group of late-produced cytokines. Kinetics and production levels of IL-6 and GM-CSF are similar in WB and PBMC cultures. In contrast, production levels of TNF-alpha and IFN-gamma are higher in WB than in PBMC whereas production levels of IL-6 and IL-2 are lower in WB than in PBMC. Individual variation in responses to PHA + LPS was always higher in PBMC cultures than in WB cultures. The capacity of cytokine production in relation to the number of mononuclear cells is higher in WB, or in PBMC having the same mononuclear cell concentration as WB, than in conventional cultures of concentrated PBMC (10(6)/ml). Because it mimics the natural environment, diluted WB culture may be the most appropriate milieu in which to study cytokine production in vitro. [less ▲]

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See detailHuman papillomavirus type 33 upstream regulatory elements can control gene expression in human cervical keratinocytes
Lauricella-Lefèbvre, M. A.; Rentier, Bernard ULg; Piette, Jacques ULg

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailUn index sélectif d’un texte philosophique. Husserl, Die Idee der Phänomenologie
Evrard, Etienne ULg; Purnelle, Gérald ULg

in Revue. Informatique et Statistique dans les Sciences Humaines (1992)

To assist the content analysis of a philosophical text, we have created a method of constituting both a selective index reduced to philosophically significant words and several frequency lists. We have ... [more ▼]

To assist the content analysis of a philosophical text, we have created a method of constituting both a selective index reduced to philosophically significant words and several frequency lists. We have applied this method to Die Idee der Phenomenologie where Husserl explains for the first time his theory of the phenomenological reduction and of the constitution of the objects in the conscience. The results proved to be valuable for the philosophical interpretation. [less ▲]

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See detailLe sexage d'embryons bovins par la technique de l'amplification en chaîne par la polymérase (acp) d'une séquence Y-spécifique bovine
Grobet, Luc ULg; Schwers, Anne; Charlier, Carole ULg et al

in Annales de Médecine Vétérinaire (1992), 136

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See detailCroissance de la productivité, rattrapage et innovation: une analyse des secteurs manufacturiers de l'OCDE
Fecher-Bourgeois, Fabienne ULg

in Economie et Prévision (1992), 102-103(1992-1/2), 117-127

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