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See detailDetection Of Apple Chlorotic Leaf Spot Virus Using A 5 ' Nuclease Assay With A Fluorescent 3 ' Minor Groove Binder-Dna Probe
Vendrame, Marina; Kummert, Jean; Lepoivre, Philippe ULg et al

in Journal of Virological Methods (2002), 104(1), 99-106

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See detailErythropoietin improves quality of life--a response.
Bottomley, Andrew; Thomas, Ronald; Van Steen, Kristel ULg et al

in Lancet Oncology (2002), 3(9), 527

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See detailIn vitro production of adaline and coccinelline, two defensive alkaloids from ladybird beetles (Coleoptera : Coccinellidae)
Laurent, Pascal ULg; Braekman, Jean-Claude; Daloze, Désiré et al

in Insect Biochemistry and Molecular Biology (2002), 32(9), 1017-1023

In vitro experiments using [1-C-14] and [2-C-14] acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7 ... [more ▼]

In vitro experiments using [1-C-14] and [2-C-14] acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7-punctata, respectively. The labelled alkaloids obtained in these experiments had a specific activity about ten times higher than that of the samples obtained in feeding experiments. This in vitro assay has enabled us to demonstrate that these two alkaloids are most likely biosynthesised through a fatty acid rather than a polyketide pathway, that glutamine is the preferred source of the nitrogen atom and that alkaloid biosynthesis takes place in the insect fat body. [less ▲]

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See detailThe role of the main silica precursor and the additive in the preparation, of low-density xerogels
Alié, Christelle ULg; Pirard, René ULg; Pirard, Jean-Paul ULg

in Journal of Non-Crystalline Solids (2002), 311(3), 304-313

The incorporation of an additive during sol-gel synthesis reduces shinkage during ambient drying. The following additives have been studied: 3-,(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3 ... [more ▼]

The incorporation of an additive during sol-gel synthesis reduces shinkage during ambient drying. The following additives have been studied: 3-,(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3-aminopropyltriethoxysilane (AES) and 3-(2-aminoethylamino)propyltriethoxysilane (EDAES) and the main silica precursors were tetraethylorthosilicate (TEOS) and tetrapropylorthosilicate (TPOS). When the additive contains methoxy groups (EDAS), it acts as a nucleation agent of the silica particles and exactly the same properties (pore volume, specific surface area, particle and aggregate size) are obtained whether the main reagent is TEOS or TPOS. The nucleation mechanism is based on the difference in reactivity between additive and main reagent. In case of nucleation by the additive, the nucleation agent fixes the properties whatever the main silica precursor is. When both the additive and the main reagent contain ethoxy groups (series AES-TEOS and EDAES-TEOS), there is no nucleation mechanism by the additive, and the silica particle size remains nearly constant. With less reactive main reagent (series AES-TPOS and EDAES-TPOS), pore volumes up to 17 cm(3)/g have been obtained with pore sizes up to nearly 10 pm and very big particles (similar to100 nm). The absence of nucleation by the additive for the couples AES-TPOS and EDAES-TPOS could be due to the fact that the difference in reactivity between ethoxy groups and propoxy groups is not sufficient to initiate the nucleation mechanism by the additive. In the absence of nucleation by the additive, the main reagent plays a role: highly porous materials with very large p. ores are prepared with TPOS. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailThe Belgian PCB/dioxin incident: analysis of the food chain contamination and health risk evaluation.
Bernard, Alfred; Broeckaert, Fabrice; De Poorter, Geert et al

in Environmental Research (2002), 88(1), 1-18

The Belgian PCB incident occurred at the end of January 1999 when a mixture of polychlorinated biphenyls (PCBs) contaminated with dioxins was accidentally added to a stock of recycled fat used in the ... [more ▼]

The Belgian PCB incident occurred at the end of January 1999 when a mixture of polychlorinated biphenyls (PCBs) contaminated with dioxins was accidentally added to a stock of recycled fat used in the production of animal feeds. Although signs of poultry poisoning were noticed by February, 1999, the source and the extent of the contamination were discovered only in May 1999, when it appeared that more than 2500 farms could have been supplied with contaminated feeds. This resulted in a major food crisis, which rapidly extended to the whole country and could be resolved only by the implementation of a large PCB/dioxin food monitoring program. Screening for PCB contamination was based on the determination of the seven PCB markers. When PCB concentrations exceeded the tolerance levels of 0.1 (milk), 0.2 (poultry, bovine, and pig meat), or 1 (animal feed) microg/g fat, dioxins (17 PCDD/Fs congeners) were also determined. At the end of December 1999, the database contained the results of more than 55,000 PCB and 500 dioxin analyses. The study of PCB levels and profiles in contaminated feeds delivered to poultry or pig farms confirmed that the Belgian PCB incident was due to a single source of PCB oil introduced into the food chain at the end of January 1999. This PCB oil had a congeners pattern closely matched to a mixture of Aroclor 1260/1254 in the proportion 75/25. The total amount of PCBs added to recycled fats was estimated at 50 kg (sum of the seven markers) or approximately 150 kg total PCBs, which corresponds to about 100 liters of PCB oil. This PCB mixture contained about 1g TEQ dioxins (more than 90- contributed by PCDFs) and about 2g TEQ dioxin-like PCBs. The proportions of PCB 52 and 101 congeners were fairly constant in animal feeds, excluding the possibility of secondary contamination due to fat recycling from contaminated animals. The highest concentrations of PCBs and dioxins were found in poultry and especially in the reproduction animals (hens and chicks), which showed the classical manifestations of chick edema disease. The pigs were also affected but to a lesser extent and no sign of intoxication was observed. The study of PCB/dioxin patterns and of the PCB:dioxin ratios revealed major differences in the metabolism of these compounds by farm animals. Whereas the PCBs:dioxins ratio was fairly constant in all poultry products with a mean value similar to that found in contaminated feeds (50,000), in pigs this ratio was both much higher and more variable (values up to 10,000,000), reflecting a faster elimination of dioxins than PCBs in these animals. These metabolic differences also emerged from the PCB and dioxin patterns which were altered much more in pigs than in poultry. Although the most contaminated food products (chicken meat) had PCB and dioxin levels more than 100 times above maximal recommended values, it is unlikely that this incident could have caused adverse effects in the general population of Belgium. A doubling of the PCB and dioxin burden of the young adult population would require the consumption of, respectively, 10 and 20 highly contaminated meals. In view of the very limited proportion of the poultry chain effectively contaminated during the incident (around 2%), such an extreme scenario was quite improbable for the general population except perhaps for farmers consuming their own products. But even in that case, it would have meant going back to the levels in the 1980s or attaining the body burden of subjects regularly eating contaminated seafood. [less ▲]

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See detailL’arrêt n°41/2002 de la Cour d’arbitrage : de la notion constitutionnelle de droit politique à la notion de "droit civil" au sens de la Convention européenne des droits de l’homme
Rosoux, Géraldine ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2002)

The article analyzes the impact of the conventional concept of "civil right" (art. 6 E.C.H.R.) on the constitutional distinction between civil and political rights (art. 144-145 Const.).

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See detailEvaluation of the Hycel Diagnostics Diana-5 Hematology Analyzer
Boemer, François ULg; Cielniaszek, Nadine; Paulus, Jean-Michel ULg et al

in Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology (2002), 8

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision ... [more ▼]

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision, linearity, and absence of carryover were all excellent. All parameters were stable for 48 hours at 4 ° C and for 8 hours at room temperature. Comparability of complete blood counts to Bayer Technicon Advia 120 measurements was highly satisfactory, with the exception of the mean cell volume, which was slightly underestimated by the Diana-5 compared to the Advia 120. Comparisons of differential leukocyte counts to 400-cell microscopic differentials were excellent for neutrophils, lymphocytes, and eosinophils and were acceptable for monocytes. The efficiency of the flagging system was high for the detection of blasts and less satisfactory for the detection of immature granulocytes and atypical lymphocytes. The instrument was reliable and easy to use. [less ▲]

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See detailAn Immunologic investigation of canine eosinophilic bronchopneumopathy.
Clercx, Cécile ULg; Peeters, Dominique ULg; German, Alex et al

in Journal of Veterinary Internal Medicine (2002), 16

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See detailChilocorine D, a novel heptacyclic alkaloid from a coccinellid beetle (Chilocorus renipustulatus)
Laurent, Pascal ULg; Braekman, Jean-Claude; Daloze, Désiré et al

in Tetrahedron Letters (2002), 43(42), 7465-7467

Chilocorine D, a new heptacyclic 'dimeric' alkaloid, has been isolated from the coccinellid beetle Chilocorus renipustulatus and its structure determined by 1D and 2D NMR analyses. It is constituted of a ... [more ▼]

Chilocorine D, a new heptacyclic 'dimeric' alkaloid, has been isolated from the coccinellid beetle Chilocorus renipustulatus and its structure determined by 1D and 2D NMR analyses. It is constituted of a hippodamine moiety linked to a modified octahydro-azaacenaphtylene skeleton encompassing a seven-membered ring. [less ▲]

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See detailColumnar defects acting as passive internal field detectors
Silhanek, Alejandro ULg; Civale, L.; Avila, M. A.

in Physical Review. B : Condensed Matter (2002), 65(17),

We have studied the angular dependence of the irreversible magnetization of several YBa2Cu3O7 and 2H-NbSe2 single crystals with columnar defects tilted off the c axis. At high magnetic fields, the ... [more ▼]

We have studied the angular dependence of the irreversible magnetization of several YBa2Cu3O7 and 2H-NbSe2 single crystals with columnar defects tilted off the c axis. At high magnetic fields, the irreversible magnetization M-i(Theta) exhibits a well-known maximum when the applied field is parallel to the tracks. As the field is decreased below Hsimilar to0.02H(c2), the peak shifts away from the tracks' direction toward either the c axis or the ab planes. We demonstrate that this shift results from the misalignment between the external and internal field directions due to the competition between anisotropy and geometry effects. [less ▲]

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See detailFurther evidence on the relationship between dopamine and novelty seeking: a neuroendocrine study
Hansenne, Michel ULg; Pinto, Emmanuel ULg; Pitchot, William ULg et al

in Personality & Individual Differences (2002), 33(6), 967-977

In the biosocial model of Cloninger, three major personality dimensions, novelty seeking (NS), harm avoidance (HA), and reward dependence (RD) are dependent on central monoaminergic systems, respectively ... [more ▼]

In the biosocial model of Cloninger, three major personality dimensions, novelty seeking (NS), harm avoidance (HA), and reward dependence (RD) are dependent on central monoaminergic systems, respectively dopaminergic, serotonergic, and noradrenergic. This study investigated the relationships between these major personality dimensions and growth hormone (GH) responses to both apomorphine and clonidine challenge tests in healthy subjects. GH responses to apomorphine were significantly correlated with NS when peak relative values were considered (r=0.47, P=0.03). HA and RD did not show any relationships with the endocrine responses. In contrast, no significant relationship existed between GH responses to clonidine and any of the three temperament dimensions. These results gave another support of the hypothesized link between NS and dopaminergic central neurotransmission. In contrast, the results did not confirm the association between RD and noradrenergic central neurotransmission, probably because RD is poorly validated. This partial confirmation might suggest that the link between personality traits and neurotransmission systems is probably indirect. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailDes risques modernes à la société réflexive : pour une autre approche du (bio)terrorisme
Claisse, Frédéric ULg; Brunet, Sébastien ULg; Rogister, Yves

in Ethique Publique, Revue internationale d’éthique sociétale et gouvernementale (2002), 4(n°2), 115-136

Au départ de quelques normes de portée nationale et internationale dont l'examen souligne la difficulté de parvenir à une définition opératoire du terrorisme, cet article propose un cadrage alternatif du ... [more ▼]

Au départ de quelques normes de portée nationale et internationale dont l'examen souligne la difficulté de parvenir à une définition opératoire du terrorisme, cet article propose un cadrage alternatif du phénomène terroriste fondé sur le paradigme des "risques réflexifs". Sous cet éclairage, le risque terroriste agit comme un véritable révélateur du rapport à notre environnement politique, social et technique: il est l'occasion de mettre en évidence des rapports de force, des relations politiques de domination et de dépendance qui constituent le contexte structurel de conflits locaux, régionaux ou internationaux; mais aussi, plus indirectement, en venant nous rappeler la vulnérabilité de nos collectifs, de plus en plus basés sur le fonctionnement en réseaux, l'interdépendance et le développement de macro-systèmes techniques. Il plaide pour une meilleure prise en compte de la dimension proprement politique du terrorisme et, plus largement, pour un basculement global vers un modèle de société ouverte plus sensible à des facteurs de risque trouvant leur origine dans nos propres pratiques et notre rapport au monde. Comme fil conducteur, des exemples empruntés au bioterrorisme, en ce que le phénomène paraît emblématique de la réflexivité des risques modernes. [less ▲]

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See detailThe Use of Cardboard Bedding Material as Part of an Environmental Control Regime for Heaves-affected Horses: In Vitro Assessment of Airborne Dust and Aeroallergen Concentration and In Vivo Effects on Lung Function
Kirschvink, Nathalie; Di silvestro, F.; Sbai, Ilham ULg et al

in Veterinary Journal (2002), 163

This study aimed to test whether shredded cardboard is an appropriate minimum-dust bedding material for heaves-affected horses. Results of standardized in vitro measurement of airborne dust and ... [more ▼]

This study aimed to test whether shredded cardboard is an appropriate minimum-dust bedding material for heaves-affected horses. Results of standardized in vitro measurement of airborne dust and aeroallergen concentrations of cardboard bedding were significantly lower than those of common bedding materials. Six heaves-affected horses in clinical remission after pasturing were stabled for two months on cardboard bedding and fed grass silage. Pulmonary function tests (PFT: ventilatory mechanics, arterial blood gases, airway inflammation scoring, bronchoalveolar cytology) were performed before, during and after this period and after stabling the horses in poor hygienic conditions. PFT values measured during and after the stabling period on cardboard bedding were not significantly different from those recorded after the period at pasture or from those of healthy horses, but were significantly different from those recorded in poor hygienic conditions. On basis of the in vitro and in vivo results it can be concluded that cardboard bedding, used in conjunction with low-dust forage, may be appropriate in the provision of minimum-dust management of heaves-affected horses [less ▲]

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See detailL'etude clinique du mois. L'etude LIPS: prevention par la fluvastatine des accidents cardiaques apres angioplastie coronaire percutanee.
Scheen, André ULg

in Revue Médicale de Liège (2002), 57(7), 479-82

The prospective placebo-controlled LIPS study ("Lescol Intervention Prevention Study") demonstrated a significant cardiovascular protection by fluvastatin in patients with coronary artery disease (stable ... [more ▼]

The prospective placebo-controlled LIPS study ("Lescol Intervention Prevention Study") demonstrated a significant cardiovascular protection by fluvastatin in patients with coronary artery disease (stable or unstable angina, silent ischemia), without major hypercholesterolaemia (135-270 mg/dl) following successful completion of their first percutaneous coronary intervention. When compared to the placebo group (n = 833), the fluvastatin group (n = 844) showed a relative risk reduction by 22% (relative risk: 0.78; 95% confidence interval: 0.64-0.95; p = 0.01) of major adverse cardiac events after a median time of follow-up of 3.9 years. This effect is observed independently of baseline total cholesterol, of the presence of diabetes mellitus or the existence of multivessel disease. These results suggest that fluvastatin may favorably influence the restenosis process after percutaneous coronary intervention, even in the absence of severe hypercholesterolaemia. [less ▲]

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See detailDiversité génétique: du diagnostic à la conservation. Le cas du vison d'Europe
Libois, Roland ULg; Michaux, J.; Rosoux, René et al

in Annales de la Société des Sciences Naturelles de la Charente-Maritime (2002), 9(2), 219-223

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See detailOutpatient and home parenteral antibiotic therapy (OHPAT) in low-risk febrile neutropenia: consensus statement of a Belgian panel.
Beguin, Yves ULg; Benoit, Yves; Crokaert, F. et al

in Acta Clinica Belgica (2002), 57(6), 309-16

Febrile neutropenia requires adequate antibiotic treatment. A subgroup of patients are only at low risk for complications and could be treated at home/as outpatients (OHPAT) after a short initial ... [more ▼]

Febrile neutropenia requires adequate antibiotic treatment. A subgroup of patients are only at low risk for complications and could be treated at home/as outpatients (OHPAT) after a short initial admission for work up. This position paper by a Belgian panel of experts presents criteria defining low-risk in febrile neutropenia, gives an overview of the existing experience and examines the present obstacles to a more widespread use of OHPAT in this country. [less ▲]

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See detailLe dépassement du délai raisonnable en matière d'infraction à la législation sur les douanes et accises
Monville, Pierre ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2002)

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See detailBiometrological assessment of skin protectors against moderate cold threat.
Claes, Geert; Pierard, Gérald ULg

in Exogenous Dermatology : Physical, Chemical, Biological (2002), 1

Background : Cold injuries represent a serious problem in winter sport practice and other outdoor activities. Some protection is expected to be achieved by specific topical preparations aiming at ... [more ▼]

Background : Cold injuries represent a serious problem in winter sport practice and other outdoor activities. Some protection is expected to be achieved by specific topical preparations aiming at decreasing heat loss by water evaporation from the skin. Objective : To compare the efficacy of 6 topical formulations enriched or not in rubefacients. The role of the vehicle was also assessed. Methods : A total of 30 trained cyclists tested the products on their forearms. The skin temperature and electrometric values were recorded at baseline following applications of the test products, and after 30 min-bicycle riding in moderately cold and humid outdoor conditions. Results : At baseline, skin temperature was increased by rubefacients dispersed a lipogel and o/w emulsion. This parameter decreased at all test sites after riding, but the values remained higher at the rubefacient-treated sites compared to the control untreated site. The oil and lipogel formulations achieved partial occlusion decreasing the transepidermal water loss. This effect was maintained after exercise without, however, hindering sweating. Conclusion : Among the presently tested formulations, the rubefacient-enriched lipogels appeared to be the most efficient formulations for maintaining skin temperature in the normal range during sport practice in a moderately cold environment. [less ▲]

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See detailStaging of regional nodes in AJCC stage I and II melanoma: 18FDG PET imaging versus sentinel node detection.
Belhocine, Tarik; Pierard, Gérald ULg; De Labrassinne, Michel et al

in Oncologist (2002), 7(4), 271-8

PRIMARY PURPOSE: The staging of regional nodes by means of sentinel node detection has been shown to accurately detect subclinical nodal metastases from cutaneous melanoma. On the other hand, the ... [more ▼]

PRIMARY PURPOSE: The staging of regional nodes by means of sentinel node detection has been shown to accurately detect subclinical nodal metastases from cutaneous melanoma. On the other hand, the oncological applications of 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18FDG PET) are, nowadays, firmly established. However, the sensitivity of such metabolic imaging for staging the regional nodes in primary melanoma remains debatable. We prospectively assessed the actual value of PET for detecting sentinel node metastases in 21 consecutive patients presenting with early-stage melanoma. MATERIALS AND METHODS: Twenty-one melanoma patients scheduled for lymphatic mapping and sentinel lymphadenectomy underwent fully corrected whole-body PET using 18FDG. In all cases, the disease was initially classified as either stage I or II, from the latest version of the American Joint Committee on Cancer staging system. The sentinel node detection was systematically performed within the week following the PET scan. Serial sections of the sentinel nodes were analyzed by both conventional pathology and immunohistochemical staining. Metastatic sentinel nodes were also assessed for the size of tumor deposits and the degree of nodal involvement (focal, partial, or massive). The median follow-up time was 12 months. RESULTS: Six of the 21 patients (28.5%) had an involved sentinel node. PET was positive in only one case with a sentinel node >1 cm. In the five other cases, the sentinel nodes missed by PET were <1 cm with focal and/or partial involvements. One patient, free of regional nodal metastases in both sentinel node detection and PET imaging, had, however, a same-basin recurrence 3 months later. In another case, PET had one false positive result. Overall, the sentinel detection of subclinical nodal metastases had a sensitivity of 86%. PET detected only 14% of sentinel node metastases. CONCLUSIONS: Sentinel node detection remains the procedure of choice for detecting subclinical lymph node involvement from primary cutaneous melanoma. Owing to its limited spatial resolution, PET appears insufficiently sensitive to identify microscopic nodal metastases. As a practical consequence, metabolic imaging is not recommended as a first-line imaging strategy for staging regional lymph nodes in patients with stage I or II melanoma. [less ▲]

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See detailMR1905401,, Khan, A. R. and Elkamel, A., Mathematical model for heat transfer mechanism for particulate System, Appl. Math. Comput.
Rochus, Pierre ULg

in Applied Mathematics and Computation (2002), 129(2-3), 295--316

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See detailAre central executive functions working in patients with focal frontal lesions?
Andrès Bénito, P.; Van der Linden, Martial ULg

in Neuropsychologia (2002), 40(7), 835-845

The aim of this study was to examine the hypothesis of a link between frontal cortex and two executive functions in working memory: the capacity to perform a dual task and the ability to inhibit ... [more ▼]

The aim of this study was to examine the hypothesis of a link between frontal cortex and two executive functions in working memory: the capacity to perform a dual task and the ability to inhibit irrelevant information. A dual task designed to assess the capacity to perform storage and processing simultaneously and a directed forgetting task designed to assess the capacity to actively inhibit no-longer relevant information were administered to a group of patients with focal frontal lesions and to a group of control participants. The results revealed that despite showing reduced short-term storage, frontal patients performed the dual task and inhibited the no-longer relevant information as well as control participants. These findings suggest that not all-executive processes are exclusively sustained by the frontal cortex [Quart J Exp Psychol 9 (1996) 5; Curr Opin Neurobiol 10 (2000) 195; Neuropsychology (1994) 544; The Cognitive Neuropsychology of Alzheimer-type dementia. Oxford, New York: Oxford University Press, 1996]. [less ▲]

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See detailCharacterisation of aphid myrosinase and degradation studies of glucosinolates
Francis, Frédéric ULg; Lognay, Georges ULg; Wathelet, Jean-Paul ULg et al

in Archives of Insect Biochemistry and Physiology (2002), 50(4), 173-182

Myrosinase from Brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a DEAE column. The chromatography yielded a single peak and a 115.6-fold purification ... [more ▼]

Myrosinase from Brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a DEAE column. The chromatography yielded a single peak and a 115.6-fold purification. Further FPLC gel filtration gave a single peak at 120 kDa. Denaturing SDS/PAGE of the protein revealed a single band at 60 kDa, indicating that the native B. brassicae myrosinase is a dimer. Kinetic parameters towards 8 glucosinolates were calculated. Strong differences of V-max and Km were observed depending on the substrate. Degradation products of each glucosinolate were identified and quantified by GC-MS and GLC-FID, respectively. Using both crude aphid homogenates and purified myrosinase, two unique hydroxyglucosinolates, 3-butenyl- and benzyl-isothiocyanates were identified from progoitrin ((2S)-2-hydroxybut-3-enyl-glucosinolate) and sinalbin (4-hydroxybenzyl-glucosinolate) degradation respectively. Addition of ascorbic acid to the reaction mixtures containing sinalbin and progoitrin caused the production of hydroxyloted degradation products usually associated with plant myrosinase metabolisation. The occurrence of the myrosinase system in B. brassicae is discussed in terms of similar allelochemical adaptation between the herbivore and its host plant. (C) 2002 Wiley-Liss, Inc. [less ▲]

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See detailTherapeutic potential of thromboxane inhibitors in asthma
Dogné, Jean-Michel ULg; De Leval, X.; Benoit, P. et al

in Expert Opinion on Investigational Drugs (2002)

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See detailBestiaire et métamorphoses d’Eugène Savitzkaya
Demoulin, Laurent ULg

in Ponts/Ponti (2002), 2

Les animaux se croisent par centaines dans les romans de Savitzkaya et leur présence a une valeur symbolique qui est analysée ici.

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See detailDust variability and land degradation in the Sahel
Ozer, Pierre ULg

in Belgeo (2002), 2002(2), 195-209

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See detailA qui appartient le ragondin ?
Roussel, Laurence; Mougenot, Catherine ULg

in Espaces et Sociétés (Paris, France) (2002), (110-111), 225-247

La lutte contre le ragondin, espèce importée d'Amérique du Sud en Europe, aujourd'hui classée parmi les espèces invasives, a été inscrite simultanément dans deux législations différentes. Dans le ... [more ▼]

La lutte contre le ragondin, espèce importée d'Amérique du Sud en Europe, aujourd'hui classée parmi les espèces invasives, a été inscrite simultanément dans deux législations différentes. Dans le mondeagricole, c'est une action qui s'inspire de la guerre contre les ravageurs des cultures. Elle s'organise collectivement à l'aide d'un poison, la bromadiolone. Dans le monde de la chasse, elle est principalement du domaine des piégeurs. Solitaires, ceux-ci pourchassent le ragondin en tant qu'espèce nuisible. Cette division est héritée du Moyen Âge, de ses usages de l'espace et des droits qui leur étaient associés (droit de destruction pour les agriculteurs). Actuellement, l'espace rural peut être vu comme une mosaïque, òu ces deux principaux types de lutte coexistent, alors que certains milieux restent libres de toute intervention. Mais ces actions sont aussi l'objet de critiques qui mettent en cause l'utilisation de la bromadiolone et certaines formes de piégeage. Et aujourd'hui, on observe que les modalités de lutte contre le ragondin se combinent aux nouveaux usages de l'espace. [less ▲]

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See detailGlutathione S-transferase isoenzymes in the two-spot ladybird, Adalia bipunctata (Coleoptera: Coccinellidae).
Francis, Frédéric ULg; Haubruge, Eric ULg; Dierickx, Paul

in Archives of Insect Biochemistry and Physiology (2002), 49(3), 158-66

Isoenzymes of glutathione S-transferase (GST) in adult Adalia bipunctata, an aphidophagous predator, were studied. Cytosolic GST activity was studied in each beetle developmental stage. The highest ... [more ▼]

Isoenzymes of glutathione S-transferase (GST) in adult Adalia bipunctata, an aphidophagous predator, were studied. Cytosolic GST activity was studied in each beetle developmental stage. The highest activities towards both 1-chloro-2,4-dinitrobenzene (CDNB) and 2,4-dinitro-1-iodobenzene (DNIB) occurred in adults. The enzyme distribution was investigated in adults. While most of the enzymatic activity was found in the abdomen (40-50 and 34-63% respectively) using several concentrations of both CDNB and DNIB, significant differences were observed for the head and the thorax depending on the substrate. Activities were more abundant in the thorax with DNIB (37-47%) compared to the 13-19% obtained with CDNB. Some GST activity was also detected in the elytra. GSTs were purified by epoxy-activated Sepharose 6B affinity chromatography and applied to an HPLC column to determine the native molecular weight (69 kDa). Three isoenzymes were separated by chromatofocusing at pH ranges 7-4. Three bands with molecular mass from 23 to 26 kDa were visualised on SDS-PAGE. Their isoelectric points were 6.66, 6.36, and 6.21. The substrate specificities and the kinetic parameters (Vm and Km) of the isoenzymes showed large differences depending on the isoenzyme. Arch. [less ▲]

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See detailAnalyse de l'impact physiologique et economique de l'elagage des arbres d'alignement en port libre.
Toussaint, André ULg; Kervyn de Meerendre, V.; Delcroix, B. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(2), 99-107

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See detailDiagnostic et traitement du syndrome hépatorénal
Gielen, Sabine; Delwaide, Jean ULg; Detry, Olivier ULg et al

in Médecine et Hygiène (2002), 60

Le syndrome hépatorénal (SHR) est une insuffisance rénale propre au patient porteur d’une hépatopathie chronique à un stade avancé. Le mécanisme physiopathologique, encore imparfaitement connu, associe ... [more ▼]

Le syndrome hépatorénal (SHR) est une insuffisance rénale propre au patient porteur d’une hépatopathie chronique à un stade avancé. Le mécanisme physiopathologique, encore imparfaitement connu, associe une vasoconstriction rénale à une vasodilatation splanchnique. Aucun examen n’est spécifique au SHR qui reste ainsi un diagnostic d’exclusion. Le pronostic du SHR est très sombre, l’évolution spontanée étant presque toujours fatale en moyenne dans le mois du diagnostic. La transplantation hépatique représente le traitement idéal mais est d’application limitée étant donné la courte survie des patients. Depuis peu, un traitement médical combinant un vasoconstricteur splanchnique, la terlipressine, et un expanseur plasmatique, l’albumine, donne des résultats favorables, permettant au patient de survivre jusqu’à la transplantation hépatique. [less ▲]

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See detailScalable adaptive hierarchical clustering
Mathy, Laurent ULg; Canonico, R.; Simpson, S. et al

in IEEE Communications Letters (2002), 6(3), 117--119

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See detailTexture evolution during deep-drawing processes
Duchene, Laurent ULg; Godinas, A.; Cescotto, Serge ULg et al

in Journal of Materials Processing Technology (2002), 125-126

This paper presents a constitutive law based on Taylor’s model implemented in our non-linear finite element code LAGAMINE. The yield locus is only locally described and a particular interpolation method ... [more ▼]

This paper presents a constitutive law based on Taylor’s model implemented in our non-linear finite element code LAGAMINE. The yield locus is only locally described and a particular interpolation method has been developed. This local yield locus model uses a discrete representation of the material’s texture. The interpolation method is presented and a deep-drawing application is simulated in order to show up the influence of the texture evolution during forming processes. [less ▲]

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See detailInfluence de l'hérédité, du sexe et de la saison sur le type d'épisode dans le trouble bipolaire.
Mortazi, M.; Bertrand, Jean-Marie ULg; Triffaux, Jean-Marc ULg et al

in Revue Médicale de Liège (2002), 57(3), 171-5

The aim of this work was to investigate if sex, age, family history, season and hypothyroidism have any influence on the type of episodes (manic, depressive, mixed) seen in bipolar patients. This ... [more ▼]

The aim of this work was to investigate if sex, age, family history, season and hypothyroidism have any influence on the type of episodes (manic, depressive, mixed) seen in bipolar patients. This retrospective study concerns a sample of 208 patients with a diagnosis of bipolar disorder (type I or II), who were admitted in one of two psychiatric centers between July 1996 and June 2000. The sex-ratio was 2.8 females for 1 male. Sex, family history and hypothyroidism were not associated with any type of episode. A higher percentage of depressive episodes was observed in the patients who were older than 50 and the average age of depressive patients was higher than that of other patients. There was no seasonal pattern in this study and the season did not influence the type of episode. The results indicate no influence of sex, season, family history and hypothyroidism on the type of episode presented. On the opposite, age seems to favour depression episode. [less ▲]

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See detailStunting may determine the severity of malaria-associated anemia in African children.
Verhoef, Hans; West, Clive E; Veenemans, Jacobien et al

in Pediatrics (2002), 110(4), 48

OBJECTIVE: Evidence from previous studies that malnourished children are protected against malaria is controversial. In individuals repeatedly exposed to malaria, immunity may develop first against severe ... [more ▼]

OBJECTIVE: Evidence from previous studies that malnourished children are protected against malaria is controversial. In individuals repeatedly exposed to malaria, immunity may develop first against severe disease, then against pyrogens, and last, against parasites. If this is true, this would suggest that reduced immune function that may exist in stunted children exacerbates the severity of malarial signs and symptoms, rather than the occurrence of parasitemia. On the other hand, several studies have suggested that malnourished children are protected to some degree against malaria. Our aim was to evaluate whether observational data support the hypothesis that nutritional inadequacies that cause stunting modify the associations between malaria and hematologic indicators such as hemoglobin concentration and serum concentrations of C-reactive protein and soluble transferrin receptor (sTfR). We showed earlier that increased serum concentrations of these receptors in asymptomatic malaria may be explained, at least in part, by increased erythropoiesis to compensate for malaria-induced hemolysis. METHODOLOGY: Community-based cluster survey among Kenyan children aged 2 to 36 months asymptomatic for malaria or anemia (n = 318). RESULTS: When adjusted for age and wasting, the malaria-associated decrease in mean hemoglobin concentration was 8.5 g/L and 15.8 g/L in nonstunted and stunted children, respectively. The malaria-associated increase in geometric mean serum concentrations of sTfR was 1.1-fold and 1.8-fold in nonstunted and stunted children, respectively. The malaria-associated increase in geometric mean serum concentrations of C-reactive protein was 1.4-fold and 2.3-fold in nonstunted and stunted children, respectively. Thus, children with malaria and those who were stunted suffered from more severe anemia and had higher serum concentrations of C-reactive protein and sTfR than would be expected from the combined effect of the 2 working independently. CONCLUSIONS: Our results are consistent with the notion that the nutritional inadequacies causing stunting also impair host immunity, thus increasing the degree to which malaria is associated with decreased concentrations of hemoglobin, with increased inflammation, and with increased iron demand in developing erythroblasts. Increased intake of micronutrients may not only reduce stunting and nutritional anemia, but also reduce malaria-associated anemia. [less ▲]

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See detailEntre dermatoses et entéropathies.
Quatresooz, Pascale ULg; Bourguignon, R.; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (2002), 57(8), 528-34

A series of gastro-intestinal diseases exhibits some connection with various cutaneous lesions. Some skin manifestations are clinically or histologically evocative, while others lack specificity. These ... [more ▼]

A series of gastro-intestinal diseases exhibits some connection with various cutaneous lesions. Some skin manifestations are clinically or histologically evocative, while others lack specificity. These manifestations can occur in overt digestive disease or be the clue for a silent one. A careful skin examination can thus reveal some inflammatory or neoplastic diseases of the gastro-intestinal tract, a malabsorption syndrome or food intolerance. [less ▲]

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See detailLes lithométéores au Niger: mise au point.
Ozer, Pierre ULg

in Würzburger Geographische Arbeiten (2002), (97), 7-32

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See detailDoes sulfide detoxication occur in the gills of the hydrothermal vent shrimp, Rimicaris exoculata?
Compère, Philippe ULg; Martinez, Anne-Sophie; Charmantier-Daures, Mireille et al

in Comptes Rendus Biologies (2002), 325

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See detailIntranasal infusion of enilconazole for treatment of sinonasal aspergillosis in dogs.
Zonderland, Jean-Luc; Stork, Christoph; Saunders, Jimmy et al

in Journal of the American Veterinary Medical Association [=JAVMA] (2002), 221

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See detailJuvenile interphalangeal degenerative joint disease in the ardenner horse: radiographical evaluation's protocol (part II)
Lejeune, Jean-Philippe ULg; Schneider, Nicole ULg; Duvivier, Dominique Hannia et al

in Annales de Médecine Vétérinaire (2002), 146(3, JUN-JUL), 173-179

Juvenile interphalangeal degenerative joint disease is frequently observed in ardenner horses. The aim of this study is to established a radiographic examination's protocol of the distal limb and to apply ... [more ▼]

Juvenile interphalangeal degenerative joint disease is frequently observed in ardenner horses. The aim of this study is to established a radiographic examination's protocol of the distal limb and to apply it to ardenner horses. This investigation method takes assess of the standings, the conformation and of the potential lesions of the digits. An essential point of this protocol is the quantification of the asymmetrical articular compression of the interphalangeal joints. Thirty-two ardenner foals of 250 +/- 21 days were radiographied. The most frequently observed anomalies are first, the existence of abnormal standings such as distal interphalangeal flexion and external articular compression of the interphalangeal joints. Second, it shows in some cases, an abnormal conformation of the second phalanx. Finally, this protocol points out the existence of juvenile degenerative joint disease and of ossification of the fibrocartilages in some foals at this age. [less ▲]

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See detailComment etablir le bilan de fin de traitement des patients atteints de lymphomes non-hodgkiniens (LNH) de malignite intermediaire ou elevee?
Warland, V.; Jerusalem, Guy ULg; Hustinx, Roland ULg et al

in Revue Médicale de Liège (2002), 57(12), 779-84

Incomplete regression of a lymphomatous mass after chemotherapy and/or radiotherapy constitutes a major problem in the treatment of lymphoma. In patients with persisting tumor, it could be reasonable to ... [more ▼]

Incomplete regression of a lymphomatous mass after chemotherapy and/or radiotherapy constitutes a major problem in the treatment of lymphoma. In patients with persisting tumor, it could be reasonable to use salvage therapy and possibly hematopoietic stem cell transplantation at the time of minimal disease rather than at the time of clinically overt relapse. The authors reviewed the most appropriate imaging techniques for the assessment of response to treatment. The limitations of CT and MRI for predicting the nature of residual masses are well known. 67Ga scintigraphy has become a standard procedure for the posttreatment evaluation of patients with lymphoma, but it appears that 18F-FDG PET may be a more effective method. Personal experience in the field of PET scan is reported. Although PET should be considered the noninvasive imaging modality of choice, a histological confirmation of residual disease is always necessary before starting salvage therapy. 18F-fluorodeoxyglucose is not a tumor specific radiotracer. [less ▲]

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See detailRhoA inhibition is a key step in pituicyte stellation induced by A(1)-type adenosine receptor activation.
Rosso, Lia; Peteri-Brunback, Brigitta; Vouret-Craviari, Valerie et al

in Glia (2002), 38(4), 351-62

Pituicyte stellation in vitro represents a useful model with which to study morphological changes that occur in vivo in these cells during times of high neurohypophysial hormone output. This model has ... [more ▼]

Pituicyte stellation in vitro represents a useful model with which to study morphological changes that occur in vivo in these cells during times of high neurohypophysial hormone output. This model has helped us establish the hypothesis of a purinergic regulation of pituicyte morphological plasticity. We first show that ATP induces stellation in 37% of pituicytes, an effect that is secondary to the metabolism of ATP to adenosine. Adenosine-induced stellation of pituicytes appears to be mediated by A(1)-type receptors. The effect is independent of intracellular calcium and does not involve the mitogen-activated protein kinase pathway. The basal (nonstellate) state of pituicytes depends on tonic activation of a Rho GTPase because both C3 transferase (a Rho inhibitor) and Y-27632 (an inhibitor of p160Rho kinase) can induce stellation. Lysophosphatidic acid, a Rho activator, blocks the morphogenic effect of adenosine dose-dependently. Using a specific RhoA pull-down assay, we also show that downregulation of activated RhoA is the key event coupling A(1) receptor activation to pituicyte stellation, via F-actin depolymerization and microtubule reorganization. Finally, both vasopressin and oxytocin can prevent or reverse adenosine-induced stellation. The effects of vasopressin, and those of high concentrations of oxytocin, are mediated through V(1a) receptors. Placed within the context of the relevant literature, our data suggest the possibility of a purinergic regulation of pituicyte morphological plasticity and subsequent modulation of hormone release, with these hormones providing a negative feedback mechanism. [less ▲]

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See detailUtilisation du bootstrap pour les problèmes statistiques liés à l'estimation des paramètres.
Palm, Rodolphe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(3),

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See detailAdult mullerian duct or utricle cyst: clinical significance and therapeutic management of 65 cases.
Coppens, Luc ULg; Bonnet, Pierre ULg; Andrianne, Robert ULg et al

in Journal of Urology (The) (2002), 167(4), 1740-4

PURPOSE: We define guidelines for the exploration and treatment of adult mullerian duct cysts. MATERIALS AND METHODS: From January 1988 through September 1999 a diagnosis of enlarged prostatic utricle was ... [more ▼]

PURPOSE: We define guidelines for the exploration and treatment of adult mullerian duct cysts. MATERIALS AND METHODS: From January 1988 through September 1999 a diagnosis of enlarged prostatic utricle was made in 65 adults based on transrectal ultrasound findings. Echographic criteria to define simple versus complicated cysts were detailed. We reviewed the clinical presentation, diagnostic modalities, indications for invasive procedures and postoperative outcome. RESULTS: The usual clinical presentations were hematospermia in 40% of cases, other ejaculatory disturbances in 20%, recurrent testicular or pelviperineal pain in 33%, lower urinary tract irritation symptoms in 25%, lower urinary tract infection in 18.5%, male infertility in 12% and incidental finding in 18.5%. Cyst dimensions did not influence the indication for invasive procedures, which were performed in only 27 of the 65 patients (41.5%) to treat disabling symptoms in 28% and obstructive infertility in 5%, and investigate complicated cysts on transrectal ultrasound in 6%. These procedures included transperineal or transrectal puncture in 9 patients, simple endoscopic section of the utricle meatus in 12 and large marsupialisation in 6. Complete and sustained cure was noted in half of the patients treated with cyst puncture only, although echographic relapse was the rule. Endoscopic procedures definitely improved or cured 82% of the patients at a mean followup of 51 months, during which neither early nor late complications were noted. CONCLUSIONS: Since almost 60% of adults diagnosed with a mullerian duct cyst did not experience any cyst related symptoms or ejaculatory-fertility impairment, we recommend that investigation and/or treatment should only be done in symptomatic or infertile patients. [less ▲]

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See detailMemory kernels and effective Hamiltonians from time dependent methods. II. Vibrational predissociation
Brems, Vincent; Desouter, Michèle ULg

in Journal of Chemical Physics (2002), 116

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See detailThe fragileX syndrome : What about the deficit innthe pragmatic component of language ?
Comblain, Annick ULg; Elbouz, Mouna

in Journal of Cognitive Education and Psychology (2002), 2(3), 29-58

Language profile of fragile-X syndrome individuals looks like the one of Down syndrome individuals, except for phonological and pragmatic abilities. If the pragmatic aspect of language is relatively ... [more ▼]

Language profile of fragile-X syndrome individuals looks like the one of Down syndrome individuals, except for phonological and pragmatic abilities. If the pragmatic aspect of language is relatively preserve in Down syndrome, it is one of the most impaired language component in fragile-X syndrome. Discourse of fragile X persons generally contains of repetitions, perseverations and stereotypies. These behaviors are well documented in the literature but, we do not know, for now, the reasons of these repetitions and perseverations. Are they the consequence of a real pragmatic disorder or the consequence of motor difficulties ? Another aspect of the pragmatic component of language remains almost unexplored in this pathology : the common ground management and the organization of the old and the new information in conversation. It is this last point that we want to explore. We decided to conduct a preliminary study with four fragile-X boys aged from 6 to 12 years-old. Groups of two boys work together on referential communication tasks. We proposed traditional experimental situations in a non-eye contact condition between participants. We compare the results of our subjects with those of typically developing children matched on the lexical age. Fragile-X boys are less efficient than typically developing children when the message to give or receive contains spatial terms or "ordinal" attributes. They also manage less efficiently with an incomplete message especially when it is given by an adult. [less ▲]

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See detailRegelgeving inzake het zelfbeschikkingsrecht, Redactie Privaat
Leleu, Yves-Henri ULg

in Tijdschrift voor Privaatrecht (2002)

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See detailRemarques sur le vocabulaire grec de la traduction
Rochette, Bruno ULg

in Revue Belge de Philologie et d'Histoire (2002), 80

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See detailContrôle de l'hypertension artérielle: peut-on faire mieux?
Krzesinski, Jean-Marie ULg

in Journal de Cardiologie = Tijdschrift voor Cardiologie (2002), 14(5), 180-181

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See detailLa nuit et le jour. L'être, l'art, l'autre selon Lévinas
Steinmetz, Rudy ULg

in Voir (2002)

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See detailL'iconographie du meurtre de saint Lambert de Liège
George, Philippe ULg

in Bulletin de la Société Royale Le Vieux-Liège (2002)

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See detailEffect of nutritional antioxidant supplementation on systemic and pulmonary antioxidant status, airway inflammation and lung function in heaves-affected horses
Kirschvink, Nathalie; Fievez, Laurence ULg; Bougnet, V. et al

in Equine Veterinary Journal (2002), 34

An oxidant/antioxidant imbalance in favour of oxidants has been identified as playing a decisive role in the pathogenesis of chronic inflammatory airway diseases. Nutritional antioxidant supplementation ... [more ▼]

An oxidant/antioxidant imbalance in favour of oxidants has been identified as playing a decisive role in the pathogenesis of chronic inflammatory airway diseases. Nutritional antioxidant supplementation might reduce oxidative damage by enhancement of the antioxidant defence, thereby modulating inflammatory processes. In a placebo-controlled, blind study, it was tested whether a dietary antioxidant supplement administered for 4 weeks would improve lung function and reduce airway inflammation in heaves-affected horses. Eight horses in clinical remission of heaves were investigated at rest and after a standardised exercise test before and after treatment with an antioxidant supplement (consisting of a mixture of natural antioxidants including vitamins E and C and selenium from a variety of sources) or placebo (oatfeed pellets without additive). Pulmonary function and exercise tolerance were monitored; systemic and pulmonary lining fluid uric acid, glutathione and 8-epi-PGF(2alpha) were analysed, and bronchoalveolar lavage (BAL) cytology and inflammatory scoring of the airways were performed. The antioxidant treatment significantly improved exercise tolerance and significantly reduced endoscopic inflammatory score. Plasma uric acid concentrations were significantly reduced, suggesting downregulation of the xanthine-dehydrogenase and xanthine-oxydase pathway. Haemolysate glutathione showed a nonsignificant trend to increase, while plasma 8-epi-PGF(2alpha) remained unchanged. Pulmonary markers and BAL cytology were not significantly affected by antioxidant supplementation. The present study suggests that the antioxidant supplement tested modulated oxidant/antioxidant balance and airway inflammation of heaves-affected horses [less ▲]

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See detailLa réforme de l'adoption pas à pas. Note sous C.A., 28 novembre 2001
Leleu, Yves-Henri ULg

in Journal des Tribunaux (2002)

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See detailRadiative transfer based scaling of LAI/FPAR retrievals from reflectance data of different resolutions.
Tian, Y; Wang, Y; Zhang, Y et al

in Remote Sensing of Environment (2002), 84(1), 143-159

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See detailThe genetic absence epilepsy rat from Strasbourg (GAERS), a rat model of absence epilepsy: Computer modeling and differential gene expression
Lakaye, Bernard ULg; Thomas, E.; Minet, Arlette ULg et al

in Epilepsia (2002), 43(Suppl. 5), 123-129

Purpose: We present results obtained by computer modeling of the thalamic network and differential gene expression analysis in a rat strain with absence epilepsy, the genetic absence epilepsy rat from ... [more ▼]

Purpose: We present results obtained by computer modeling of the thalamic network and differential gene expression analysis in a rat strain with absence epilepsy, the genetic absence epilepsy rat from Strasbourg (GAERS). Methods: (a) Computer modeling used equations from the Hodgking-Huxley model with a circuit of 13 reticular thalamic (nRt) and 39 thalamocortical (TC) neurons; (b) gene-expression analysis using differential mRNA display (DD), in situ hybridization, Northern blotting, and competitive reverse transcriptase-polymerase chain reaction (RT-PCR). Results: (a) Computer modeling showed an increased network synchrony in the thalamic circuit as the value of conductance of low-voltage activated calcium channel (LVACC) is increased. (b) Using differential mRNA display, a 40% upregulation of the H-ferritin mRNA in the hippocampus was demonstrated. Looking for some candidate genes of the VACC family, no difference was found in the alpha1G mRNA expression between GAERS and control animals, whereas a decreased expression of the alpha1E subunit was observed in the cerebellum and the brainstem of the GAERS. This phenomenon was not observed in young animals when the epileptic phenotype is not expressed. Conclusions: The use of computer modeling appeared to be an efficient way to evaluate the impacts of electrophysiologic findings in vivo from single cells on an entire circuit. No clear single gene defect was revealed so far in GAERS. More information could arise from linkage analysis. However, some brain structures like hippocampus or cerebellum classically not known to be involved in the production of absence spike-and-wave discharges could in fact participate in the development of this phenotype. [less ▲]

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See detailPhylogeny and Morphological Evolution of the Amblystegiaceae (Bryopsida)
Vanderpoorten, Alain ULg; Hedenäs, L.; Cox, C. J. et al

in Molecular Phylogenetics & Evolution (2002), 23

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See detailRenouvellement des pédagogies musicales. Analyse et développement des innovations dans les cours d'instruments semi-collectifs
Giot, Bernadette ULg; Grifnée, Catherine

in Site du Ministère de la Communauté française de Belgique (2002)

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See detaille nouvel article 65, alinéa 2 du code pénal et son application dans le temps
Monville, Pierre ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2002)

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See detailStructural approach of the mechanism of inhibition of -chymotrypsin by coumarins
Wouters, J.; Huygens, M.; Pochet, L. et al

in Bioorganic & Medicinal Chemistry Letters (2002), 12

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See detailTaxonomic assessment, distribution, and ecology of Tortula vahliana var. minor (Pottiaceae, Bryopsida)
Andriessen, L.; Nagels, C.; Arts, T. et al

in Journal of Bryology (2002), 24

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See detailAsymmetrical international attitudes
Van Oudenhoven, J.; Askevis-Leherpeux, F.; Hannover, B. et al

in European Journal of Social Psychology (2002), 32(2), 275-289

In general, attitudes towards nations have a fair amount of reciprocity: nations either like each other are relatively indifferent to each other or dislike each other Sometimes, however international ... [more ▼]

In general, attitudes towards nations have a fair amount of reciprocity: nations either like each other are relatively indifferent to each other or dislike each other Sometimes, however international attitudes are asymmetrical. In this study, we use social identity theory in order to explain asymmetrical attitudes. Parting from social identity theory, asymmetrical attitudes can be predicted to occur most likely between countries that are linguistically either similar or closely related, but differ in size. Europe, more than any other continent, offers a rich variety of nations which represent natural conditions for our study, such as size and degree of linguistic similarity. In order to test hypotheses derived from social identity theory, we asked respondents (N = 405) from (Dutch- and French-speaking) Belgium, France, Germany, and The Netherlands to fill out a questionnaire on three large nations (Germany, Great Britain, France) and three smaller ones (The Netherlands, Belgium, and Denmark). Results strongly supported hypotheses and confirm that a social identity approach may help to better understand international attitudes. [less ▲]

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See detailPolyester layered silicate nanohybrids by controlled grafting polymerization
Lepoittevin, Bénédicte; Pantoustier, Nadège; Alexandre, Michaël ULg et al

in Journal of Materials Chemistry (2002), 12(12), 3528-3535

Poly( epsilon-caprolactone) (PCL) layered silicate nanohybrids were synthesized by ring opening polymerization of epsilon-caprolactone according to a well-controlled coordination-insertion mechanism ... [more ▼]

Poly( epsilon-caprolactone) (PCL) layered silicate nanohybrids were synthesized by ring opening polymerization of epsilon-caprolactone according to a well-controlled coordination-insertion mechanism. Montmorillonites were surface-modified by non functional (trimethylhexadecylammonium) and hydroxy functional alkylammonium cations, i.e., (2-hydroxyethyl) dimethylhexadecylammonium. The hydroxy functions available at the clay surface were activated into tin( II or IV) or Al(III) alkoxide initiators for lactone polymerization, thus yielding surface-grafted PCL chains. The surface-grafted PCL chains were recovered by an ionic exchange reaction with lithium chloride and they were analyzed by size exclusion chromatography. The PCL molar mass was measured as a function of the hydroxy content of the clay that was modulated by exchanging the Na cations with mixtures of non-functional and hydroxy functional ammonium cations of different compositions. Nanohybrids were also characterized by small-angle X-ray diffraction, transmission electron microscopy and thermogravimetry. The PCL molar mass and the nanocomposite morphology (i.e., exfoliation and/or intercalation) were readily tuned by the content of the hydroxy groups available at the clay surface. Surface-grafted aluminium trialkoxide species proved highly efficient in initiating polymerization that leads to PCL chains of controlled molar mass and narrow molecular weight distribution with polydispersity indices as low as 1.2. [less ▲]

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See detailDifferential pathomechanisms of epidermal necrolytic blistering diseases.
Paquet, Philippe ULg; Pierard, Gérald ULg

in International Journal of Molecular Medicine (2002), 10(6), 695-9

Staphylococcal scalded skin syndrome (SSSS) results from the effect of exfoliative-toxins produced by staphylococcal strains. The disease affects predominantly children, and is rare in adults. We report ... [more ▼]

Staphylococcal scalded skin syndrome (SSSS) results from the effect of exfoliative-toxins produced by staphylococcal strains. The disease affects predominantly children, and is rare in adults. We report two cases of the adult type of SSSS. Corticotherapy, chronic alcohol abuse and epilepsy-related immune changes might have been predisposing factors in these patients. The immunopathological characteristics of the inflammatory cell infiltrate in adults SSSS have not been thoroughly explored so far in the literature. Biopsies from 2 patients with bullous SSSS skin were studied by means of immunochemistry using a panel of 10 antibodies directed to FXIIIa, CD15, CD31, CD45R0, CD50, CD54, CD62E, CD95, CD106, and L1-protein, respectively. Cutaneous biopsies from related blistering diseases were compared. They included drug-induced toxic epidermal necrolysis (TEN), bullous impetigo and superficial pemphigus. A dense cell infiltrate composed of granulocytes (CD15+), macrophages (L1 protein+) and memory T cells (CD45R0+) and a strong expression of ICAM-3 (CD50) were present in the epidermis. CD95+ keratinocytes were lining the intraepidermal blisters. Type I dermal dendrocytes (Factor XIIIa+) were numerous and plump in the dermis. Bullous impetigo exhibited the same pattern of inflammatory cells, but with a lower density in type I dermal dendrocytes. TEN differed from SSSS by both the absence of CD15+ granulocytes and a stronger expression of the pro-apoptotic CD95 antigen in the epidermis. In superficial pemphigus, CD95 antigen was not expressed, and CD15+ granulocytes, CD45R0+ lymphocytes and L1 protein+ monocytes were much less numerous. It is concluded that the specific binding of SSSS-induced exotoxins to the desmosomes alters the keratinocyte metabolism leading to an inflammatory reaction followed by focal apoptosis. Our findings are in line with the concept that SSSS exotoxins might be superantigens. A common pathomechanism leading to epidermal destruction is likely operative in SSSS and bullous impetigo. The inflammatory cell composition in TEN and superficial pemphigus markedly differs from that in SSSS. [less ▲]

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See detailDesign of a foil-coiled inductor for the heating of steel wires
De coster, Jeroen; Deliège, Geoffrey ULg; Henrotte, François et al

in IEE Proceedings - Science, Measurement & Technology (2002), 149(5), 203-206

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See detailSaberes e instrumentos para ler os territórios próximos e distantes
Merenne-Schoumaker, Bernadette ULg

in Educação Geográfica (2002), (15), 43-54

Cet article rend compte de l'expérience de la Fédération des Professeurs de Géographie de la Communauté française de Belgique pour aider à la mise en place d'une pédagogie par compétences et plus ... [more ▼]

Cet article rend compte de l'expérience de la Fédération des Professeurs de Géographie de la Communauté française de Belgique pour aider à la mise en place d'une pédagogie par compétences et plus spécifiquement du guide conceptuel que nous avons rédigé pour apprendre aux élèves à lire les territoires du monde [less ▲]

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See detailAre all glitazones the same?
Van Gaal, Luc; Scheen, André ULg

in Diabetes/Metabolism Research & Reviews (2002), 18 Suppl 2

This supplement focuses on the benefits of targeting insulin resistance through therapy with a new class of oral antidiabetic agents, the thiazolidinediones (TZDs) or 'glitazones'. There are important ... [more ▼]

This supplement focuses on the benefits of targeting insulin resistance through therapy with a new class of oral antidiabetic agents, the thiazolidinediones (TZDs) or 'glitazones'. There are important differences between the three TZD class members that warrant discussion to enable physicians to make rational and informed therapeutic choices between the agents. Overall the TZDs appear to be similar in their effects on blood glucose, as all class members have demonstrated effective glycaemic control, both as monotherapy and in combination with sulphonylureas, metformin or exogenous insulin. The safety profiles of the three agents are more diverse, with what appear to be 'TZD class effects', (probably mediated via activation of peroxisome proliferator-activated receptor gamma [PPAR gamma]) and 'TZD-specific effects', which are unique to each agent and may be a consequence of differing chemical structures. While rosiglitazone and pioglitazone share some class effects with troglitazone, they have several characteristics that define them as unique agents. By tackling the control of type 2 diabetes through direct effects on insulin resistance, the TZDs represent an important new therapeutic tool for healthcare professionals. [less ▲]

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See detailComment on "Strong vortex liquid correlation from multiterminal measurements on untwinned YBa2Cu3O7-delta single crystals"
Maiorov, B.; Silhanek, Alejandro ULg; de la Cruz, F. et al

in Physical Review Letters (2002), 88(13),

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See detailThe formation and function of extracellular enveloped vaccinia virus
Smith, G. L.; Vanderplasschen, Alain ULg; Law, M.

in Journal of General Virology (The) (2002), 83

Vaccinia virus produces four different types of virion from each infected cell called intracellular mature virus (IMV), intracellular enveloped virus (IEV), cell-associated enveloped virus (CEV) and ... [more ▼]

Vaccinia virus produces four different types of virion from each infected cell called intracellular mature virus (IMV), intracellular enveloped virus (IEV), cell-associated enveloped virus (CEV) and extracellular enveloped virus (EEV). These virions have different abundance, structure, location and roles in the virus life-cycle. Here, the formation and function of these virions are considered with emphasis on the EEV form and its precursors, IEV and CEV. IMV is the most abundant form of virus and is retained in cells until lysis; it is a robust, stable virion and is well suited to transmit infection between hosts. IEV is formed by wrapping of IMV with intracellular membranes, and is an intermediate between IMV and CEV/EEV that enables efficient virus dissemination to the cell surface on microtubules. CEV induces the formation of actin tails that drive CEV particles away from the cell and is important for cell-to-cell spread. Lastly, EEV mediates the long-range dissemination of virus in cell culture and, probably, in vivo. Seven virus-encoded proteins have been identified that are components of IEV, and five of them are present in CEV or EEV. The roles of these proteins in virus morphogenesis and dissemination, and as targets for neutralizing antibody are reviewed. The production of several different virus particles in the VV replication cycle represents a coordinated strategy to exploit cell biology to promote virus spread and to aid virus evasion of antibody and complement. [less ▲]

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See detailShunt cardiaque droite-gauche : étiologie possible de migraine avec aura ?
Schoenen, Jean ULg

in Migraine Contact (2002), 2

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See detailUne étude inédite de Franz Cumont sur le vocabulaire des inscriptions funéraires chrétiennes de Gaule
Rochette, Bruno ULg

in Bulletin de l'Institut Historique Belge de Rome (2002), 72

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See detailFast F-18 FDG synthesis by alkaline hydrolysis on a low polarity solid phase supports
Lemaire, Christian ULg; Damhaut, P.; Lauricella, Benjamino ULg et al

in Journal of Labelled Compounds & Radiopharmaceuticals (2002), 45(5), 435-447

The synthesis of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) has been simplified by the use of a tC18 Sep Pak cartridge to effect purification and hydrolysis of the tetraacetylated [18F]fluoro-glucose ... [more ▼]

The synthesis of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) has been simplified by the use of a tC18 Sep Pak cartridge to effect purification and hydrolysis of the tetraacetylated [18F]fluoro-glucose compound ([18F]TAG). After radiolabelling, this derivative was trapped on a solid phase extraction (SPE) cartridge and the residual reaction solvent (CH3CN), reagents (K222, K2CO3,) and by-products removed by washing the support with water. After this cleaning step, the acetyl groups were cleaved on the same tC18 column using 2N sodium hydroxide. This fast reaction proceeded near quantitatively (>98%) at room temperature in less than 2 min. The [18F]FDG was then recovered with a small amount of water, neutralized with a slight excess of 2N hydrochloric acid, buffered for pH with a citrate solution and finally purified on a neutral alumina oxide and a second tC18 column. After filtration, the radiochemical yield of this [18F]FDG isotonic solution after more than 100 production runs was found to be very reliable and reproducible (70±6% decay corrected). The synthesis time was about 22 min. Quality controls showed that the radiochemical purity was higher than 98% and in any case no [18F]FDM was detected. Only traces of 2-chloro-2-deoxy-glucose (ClDG) were found in the final sample (64±9 g/ batch of 16 ml). [18F]FDG specific activity averaged between 1 and 20 Ci/µmol (EOS). No evaporation and use of ion retardation resin (AG11A8) are required. Moreover, this new approach is suitable for complete remote operation using available single use medical components. Copyright © 2002 John Wiley [less ▲]

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See detailRisk factors for hepatitis C: past, present and future
Delwaide, Jean ULg; Bourgeois, N.; Colle, I. et al

in Acta Gastro-Enterologica Belgica (2002), 65(2), 87-89

Patients at risk for hepatitis C (HCV) are those exposed to a major risk factor (i.e. blood transfusion prior 1990, and intravenous drug abuse), and those exposed to a minor risk factor (sexual, mother-to ... [more ▼]

Patients at risk for hepatitis C (HCV) are those exposed to a major risk factor (i.e. blood transfusion prior 1990, and intravenous drug abuse), and those exposed to a minor risk factor (sexual, mother-to-infant transmission, household contact, nosocomial contamination). The present paper aims to review the current and past modes of transmission of the virus C. [less ▲]

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See detailCloning and characterization of ADAMTS-14, a novel ADAMTS displaying high homology with ADAMTS-2 and ADAMTS-3.
Colige, Alain ULg; Vandenberghe, Isabel; Thiry, Marc ULg et al

in Journal of Biological Chemistry (2002), 277(8), 5756-66

The processing of amino- and carboxyl-propeptides of fibrillar collagens is required to generate collagen monomers that correctly assemble into fibrils. Mutations in the ADAMTS2 gene, the ... [more ▼]

The processing of amino- and carboxyl-propeptides of fibrillar collagens is required to generate collagen monomers that correctly assemble into fibrils. Mutations in the ADAMTS2 gene, the aminopropeptidase of procollagen I and II, result in the accumulation of non-fully processed type I procollagen, causing human Ehlers-Danlos syndrome type VIIC and animal dermatosparaxis. In this study, we show that the aminopropeptide of type I procollagen can be cleaved in vivo in absence of ADAMTS-2 activity and that this processing is performed at the cleavage site for ADAMTS-2. In an attempt to identify the enzyme responsible for this alternative aminoprocollagen peptidase activity, we have cloned the cDNA and determined the primary structure of human and mouse ADAMTS-14, a novel ADAMTS displaying striking homologies with ADAMTS-2 and -3. The structure of the human gene, which maps to 10q21.3, and the mechanisms of generation of the various transcripts are described. The existence of two sites of initiation of transcription, in two different promoter contexts, suggests that transcripts resulting from these two sites can be differently regulated. The tissue distribution of ADAMTS-14, the regulation of the gene expression by various cytokines and the activity of the recombinant enzyme are evaluated. The potential function of ADAMTS-14 as a physiological aminoprocollagen peptidase in vivo is discussed. [less ▲]

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See detailLe contrat de travail et la signature électronique
Kefer, Fabienne ULg; Barth, H.

in Orientations : la Revue du Droit Social et de la Gestion du Personnel (2002)

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See detailDNA immunisation. New histochemical and morphometric data.
Ehirchiou, D.; Zorzi, Willy ULg; Biemans, R. et al

in European Journal of Histochemistry (2002), 46(3), 215-22

Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the ... [more ▼]

Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the SAG1 protein, a membrane antigen of Toxoplasma gondii. Specific anti-SAG1 antibodies were detected on days 16 and 36 after injection of coding plasmids. The results of ELISAs showed that the SAG1-specific antibodies are of the IgG2a class. Morphometric analyses were done on serial immunostained cryosections of spleen and draining or non-draining lymph nodes. This new approach made it possible to evaluate the chronological changes induced by DNA immunisation in the germinal centres (in number and in size). Significant increases in the number of germinal centres were measured in the spleen and only in draining lymph nodes after plasmid injection, the measured changes of the germinal centers appeared to result from the adjuvant stimulatory effect of the plasmidic DNA since both the coding and the noncoding plasmid DNA induced them. No measurable changes were recorded in the T-dependent zone of lymph organs. [less ▲]

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See detailLa dignité humaine comme concept juridique
Fierens, Jacques ULg

in Journal des Tribunaux (2002)

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See detailDevelopment and validation of a new Fourier transform infrared spectrometric method for the quantification of urea in creams and ointments
Otte, X; Evrard, Brigitte ULg; Delattre, Luc ULg et al

in Analytica Chimica Acta (2002), 451

A new Fourier transform infrared (FTIR) spectrometric method for the quantification of urea in creams and ointments was developed and validated. The sample was solubilized in a mixture of chloroform ... [more ▼]

A new Fourier transform infrared (FTIR) spectrometric method for the quantification of urea in creams and ointments was developed and validated. The sample was solubilized in a mixture of chloroform/acetonitrile (1/1 v/v) which allows to solubilize the vaseline or other fats as well as urea and water. The solution was examined in a transmission cell with a pathlength of 500 μm provided with NaCI windows. The urea spectrum was obtained by subtraction of the reference solvent mixture spectrum and the reference water spectrum from the sample spectrum. The absorbance of the peak at ±1688 cm-1 was compared to those of calibration standards to quantify urea. The detection limit and the quantification limit (three times and 10 times the noise, respectively) were estimated as 0.4 and 1.3 μg ml-1, respectively. Validation of the method was realized and application of this method to stability tests was done. [less ▲]

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See detailThe effect of pH on the alternative oxidase activity in isolated Acanthamoeba castellanii mitochondria.
Jarmuszkiewicz, W.; Hryniewiecka, L.; Sluse, Francis ULg

in Journal of Bioenergetics & Biomembranes (2002), 34(3), 221-226

Mitochondria of Acanthamoeba castellanii possess a cyanide-resistant GMP-stimulated ubiquinol alternative oxidase in addition to the cytochrome pathway. In a previous work it has been observed that an ... [more ▼]

Mitochondria of Acanthamoeba castellanii possess a cyanide-resistant GMP-stimulated ubiquinol alternative oxidase in addition to the cytochrome pathway. In a previous work it has been observed that an interaction between the two ubiquinol-oxidizing pathways exists in intact A. castellanii mitochondria and that this interaction may be due to a high sensitivity of the alternative oxidase to matrix pH. In this study we have shown that the alternative oxidase activity reveals a pH-dependence with a pH optimum at 6.8 whatever the reducing substrate may be. The GMP stimulation of alternative oxidase is also strongly dependent on pH implicating probably protonation/deprotonation processes at the level of ligand and protein with an optimum pH at 6.8. The ubiquinone redox state-dependence of alternative oxidase activity is modified by pH in such a way that the highest activity for a given ubiquinone redox state is observed at pH 6.8. Thus pH, binding of GMP, and redox state of ubiquinone collaborate to set the activity of the GMP-stimulated alternative oxidase in isolated A. castellanii mitochondria. The high pH sensitivity of the alternative oxidase could link inactivation of the cytochrome pathway proton pumps to activation of the alternative oxidase with acceleration of redox free energy dissipation as a consequence. [less ▲]

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See detail2-Alkyl-3-alkylamino-2H-benzo- and pyridothiadiazine 1,1-dioxides: from K+ATP channel openers to Ca++ channel blockers?
Ouedraogo, R.; Becker, B.; Boverie, S. et al

in Biological Chemistry (2002), 383

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See detailL'etude clinique du mois. La MRC/BHF Heart Protection Study.
Kulbertus, Henri ULg; Scheen, André ULg

in Revue Médicale de Liège (2002), 57(9), 613-6

20,536 adults (15,454 men and 5,082 women, aged 40-80 years) with coronary heart disease, other occlusive arterial disease or diabetes mellitus were randomly allocated to receive 40 mg simvastatin daily ... [more ▼]

20,536 adults (15,454 men and 5,082 women, aged 40-80 years) with coronary heart disease, other occlusive arterial disease or diabetes mellitus were randomly allocated to receive 40 mg simvastatin daily or matching placebo. In addition to being randomized to compare simvastatin vs placebo, all patients were also randomized to compare antioxidant vitamin supplementation (vitamin E 600 mg/day, vitamin C 250 mg/day and betacarotene 20 mg/day) vs placebo in a "2 x 2 factorial" design. Duration of the study was 5 years. All-cause mortality was significantly reduced among patients allocated to simvastatin (-12.3%) due to a highly significant (-18%) reduction in the coronary death rate, a marginally significant reduction in other vascular deaths and a non-significant reduction in non-vascular deaths. There were highly significant reductions (of about one-quarter) in the first event rate for non-fatal myocardial infarction and coronary death (combined), for non-fatal and fatal stroke and for coronary or non-coronary revascularization. The beneficial effect of simvastatin was seen in all sub-categories which were studied and, particularly: women vs men; patients aged > 70 years vs those aged < 70 years; patients with LDL cholesterol < vs > 116 mg/dl, or total cholesterol < vs > 193 mg/dl. The treatment was well tolerated and the annual risk of myopathy was 0.01%. All comparisons between antioxidant vitamin supplementation and placebo failed to reveal any difference in favour or against the supplementation which was otherwise well tolerated. These important results and their implications will be briefly discussed. [less ▲]

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See detailBeyond a glimpse at seasonal dry skin. A review.
Franchimont, Claudine ULg; Pierard, Gérald ULg

in Exogenous Dermatology : Physical, Chemical, Biological (2002), 1

On clinical grounds the so-called dry skin corresponds in reality to a rough sometimes flaky and scaly stratum corneum. The spectrum of xerotic disorders is quite large encompassing diverse etiologies and ... [more ▼]

On clinical grounds the so-called dry skin corresponds in reality to a rough sometimes flaky and scaly stratum corneum. The spectrum of xerotic disorders is quite large encompassing diverse etiologies and physiopathological pathways. The clinical assessment of these conditions relies on close visual and tactile inspection. EEMCO guidances were proposed in this field for subject and expert evaluations. Scoring systems include analogue scales, the overall dry skin score (ODS) and the SRRC score combining the grading of scaliness, roughness, redness and cracks. The dry skin extent area and severity index (DASI) is defined for the dermatological evaluation of the entire body surface. The use of a video camera equipped with an internal ultraviolet light illumination unit is very informative and allows better comparative assessments. [less ▲]

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See detailStability of recombinant 2 S albumin allergens in vitro
Murtagh, Gareth; Dumoulin, Mireille ULg; Alcocer, Marcos et al

in Biochemical Society Transactions (2002), 30

Two well known 2 S albumins, Ber e 1 from brazil nut and sunflower 2 S albumin 8 (SFA-8), have been expressed in a eukaryotic system and purified. Analysis of recombinant versions of Ber e 1 and SFA-8 ... [more ▼]

Two well known 2 S albumins, Ber e 1 from brazil nut and sunflower 2 S albumin 8 (SFA-8), have been expressed in a eukaryotic system and purified. Analysis of recombinant versions of Ber e 1 and SFA-8 revealed them to be significantly more resistant to digestion by pepsin than BSA, and to be stable for up to 30 min in simulated gastric fluid. Unfolding monitored by CD indicated that both proteins were also very resistant to denaturation induced by heat and low pH. These results suggest that, although the ability of 2 S albumins to reach the circulatory system may be a prerequisite for the allergenicity of this group of proteins, stability is just one of a number of characteristics that provoke a selective immune response. [less ▲]

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See detailIn vitro and in vivo effects of new insulin releasing agents
Nguyen, Q. A.; Antoine, M. H.; Ouedraogo, R. et al

in Biochemical Pharmacology (2002)

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See detailConfirmation des effets délétères rénaux des AINS anti COX2 illustrée par trois cas cliniques
Piront, P.; Krzesinski, Jean-Marie ULg

in Acta Clinica Belgica (2002), 57(1), 35

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See detailRéanimation cardiopulmonaire chez la femme enceinte
Brichant, Jean-François ULg; Dewandre, Pierre-Yves ULg; Bonhomme, Vincent ULg et al

in Praticien en Anesthésie Réanimation (Le) (2002), 6

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See detailActualites therapeutiques 2002 (1).
Scheen, André ULg

in Revue Médicale de Liège (2002), 57(11), 719-31

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The main characteristics of each molecule and ... [more ▼]

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The main characteristics of each molecule and its modalities of appropriate use in clinical practice will be emphasized. For all these molecules, both the efficacy and tolerance have been proven in randomised clinical trials according to the rules of Evidence-Based Medicine. [less ▲]

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See detailSite-directed mutagenesis studies of the hypoxia-inducible factor-1alpha DNA-binding domain.
Michel, Gaetan; Minet, Emmanuel; Mottet, Denis ULg et al

in Biochimica et Biophysica Acta (2002), 1578(1-3), 73-83

Hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor, is activated when cells are exposed to hypoxia. It is composed of two subunits, HIF-1alpha and ARNT. When activated, it binds to ... [more ▼]

Hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor, is activated when cells are exposed to hypoxia. It is composed of two subunits, HIF-1alpha and ARNT. When activated, it binds to the hypoxia-responsive element (HRE) and up-regulates the expression of several genes (vascular endothelial growth factor (VEGF), erythropoietin (EPO), enolase, em leader ). By molecular modeling, a 3-D model for the complex between the DNA-binding domain of HIF-1 (bHLH domain) and its consensus DNA sequence has been developed. Specific interactions between three amino acids (Ser22, Ala25, Arg30) of the HIF-1alpha subunit and DNA bases were identified. In order to further investigate the role of these amino acids, we generated four mutants of the HIF-1alpha subunit using site-directed mutagenesis. The activity of each mutant was tested using a reporter gene containing either 6 HRE sequences or the authentic human VEGF promoter. The results show that three mutants, Ala25Ser, Ala26Glu and Arg30Ala, were no longer active in the reporter gene assay. On the other hand, the Ser22Ala mutant increased the reporter gene expression, in normoxia as well as in hypoxia. These results correlate with the ones obtained when the DNA-binding capability of the mutants was assayed by electrophoretic mobility shift assays (EMSA): Arg30Ala and Ala26Glu mutants bind very weakly to HRE while the Ser22Ala mutant has the same binding capacity as the wild-type HIF-1alpha. These results bring new insights on the specificity of the protein/DNA interactions for HIF-1 towards HRE. [less ▲]

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See detailRésumé C.E. n°97.612, du 10 juillet 2001, SA ITM Belgium
Delnoy, Michel ULg

in Aménagement - Environnement (2002), 1

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See detailInfluence Of A New Axial Impeller On K(L)A And Xylanase Production By Penicillium Canescens 10-10c
Bakri, Yasser; Jacques, Philippe ULg; Shi, Kui et al

in Applied Biochemistry and Biotechnology (2002), 98

The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, KLa, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one ... [more ▼]

The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, KLa, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one with one DT4 impeller below and one HTPG4 above (DT4-HTPG4) and one with two DT4 (DT4-DT4) impellers, in a 5-L bioreactor. The volumetric coefficient of oxygen transfer was measured in culture medium using a gassing-out method at different gassing rates and agitation speeds. We observed that the DT4-HTPG4 combination provided better KLa performance than the DT4-DT4 combination. The two combinations were also tested for their influence on xylanase production by a filamentous microorganism; P. canescens 10-10c. These experiments demonstrated that the DT4-HTPG4 combination impeller enhanced enzyme production up to 23% compared with the DT4-DT4 combination at an aeration rate of 1 vvm and an agitation speed of 600 rpm. The main cause for this difference is thought to be a higher shear stress generated by the DT4-DT4 combination, which damages the mycelium of P. canescens and decreases xylanase production. [less ▲]

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See detailLe medicament du mois. La rosiglitazone (Avandia).
Scheen, André ULg

in Revue Médicale de Liège (2002), 57(4), 236-9

Rosiglitazone (Avandia, Glaxo-SmithKline) belongs to a new family of oral hypoglycaemic agents, thiazolidinediones or glitazones. These molecules act as selective agonists of nuclear receptors (PPAR gamma ... [more ▼]

Rosiglitazone (Avandia, Glaxo-SmithKline) belongs to a new family of oral hypoglycaemic agents, thiazolidinediones or glitazones. These molecules act as selective agonists of nuclear receptors (PPAR gamma) and improve insulin sensitivity. In Belgium as in all European countries, rosiglitazone is indicated for the treatment of type 2 diabetes, only in combination with another antidiabetic oral agent, in patients insufficiently controlled with metformin or a sulphonylurea at a maximal tolerated dose. In these patients, rosiglitazone, at a daily dose of 4 mg (sometimes 8 mg/day with metformin), reduces fasting glycaemia by 2-3 mmol/l and glycated haemoglobin level by about 1%. It exerts also favourable effects on some risk factors related to insulin resistance syndrome, which may contribute to improve cardiovascular prognosis of patients with type 2 diabetes. Hepatic safety of rosiglitazone seems to be good, although it is still recommended to check liver enzymes regularly. As all glitazones, rosiglitazone moderately promotes weight gain. It can also induce some fluid retention which may reveal or aggravate heart failure in at risk patients. [less ▲]

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See detailPre- and post-harvest practical application of Pichia anomala strain K, beta-1,3-glucans and calcium chloride on apples: two years of monitoring and efficacy against post-harvest diseases.
Jijakli, Mohamed ULg; de Clercq, Deborah; Dickburt, Catherine et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2002), 25(10), 29-32

Detailed reference viewed: 28 (1 ULg)