References of "Article"
     in
Bookmark and Share    
See detailLe pilotage de l'enseignement des sciences : un essai de synthèse...
Rousselet, S.; Monseur, Christian ULg

in Informations pédagogiques (1993), 9

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailRetinoic acid induction of major histocompatibility complex class I genes in NTera-2 embryonal carcinoma cells involves induction of NF-kappa B (p50-p65) and retinoic acid receptor beta-retinoid X receptor beta heterodimers.
Segars, J. H.; Nagata, T.; Bours, Vincent ULg et al

in Molecular & Cellular Biology (1993), 13(10), 6157-69

Retinoic acid (RA) treatment of human embryonal carcinoma (EC) NTera-2 (NT2) cells induces expression of major histocompatibility complex (MHC) class I and beta-2 microglobulin surface molecules. We found ... [more ▼]

Retinoic acid (RA) treatment of human embryonal carcinoma (EC) NTera-2 (NT2) cells induces expression of major histocompatibility complex (MHC) class I and beta-2 microglobulin surface molecules. We found that this induction was accompanied by increased levels of MHC class I mRNA, which was attributable to the activation of the two conserved upstream enhancers, region I (NF-kappa B like) and region II. This activation coincided with the induction of nuclear factor binding activities specific for the two enhancers. Region I binding activity was not present in undifferentiated NT2 cells, but binding of an NF-kappa B heterodimer, p50-p65, was induced following RA treatment. The p50-p65 heterodimer was produced as a result of de novo induction of p50 and p65 mRNAs. Region II binding activity was present in undifferentiated cells at low levels but was greatly augmented by RA treatment because of activation of a nuclear hormone receptor heterodimer composed of the retinoid X receptor (RXR beta) and the RA receptor (RAR beta). The RXR beta-RAR beta heterodimer also bound RA responsive elements present in other genes which are likely to be involved in RA triggering of EC cell differentiation. Furthermore, transfection of p50 and p65 into undifferentiated NT2 cells synergistically activated region I-dependent MHC class I reporter activity. A similar increase in MHC class I reporter activity was demonstrated by cotransfection of RXR beta and RAR beta. These data show that following RA treatment, heterodimers of two transcription factor families are induced to bind to the MHC enhancers, which at least partly accounts for RA induction of MHC class I expression in NT2 EC cells. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailCaractéristiques du nid et du site de nidification chez le grèbe huppé (Podiceps cristatus). Influence sur le succès des éclosions
Marechal, Corinne ULg

in Cahiers d'Ethologie (1993), 13(1), 1-34

The study aimed at determining the influence of nest and nest-site characteristics on breeding success in a population of great Crested grebes (Podiceps cristatus) nesting on an old máeander of the river ... [more ▼]

The study aimed at determining the influence of nest and nest-site characteristics on breeding success in a population of great Crested grebes (Podiceps cristatus) nesting on an old máeander of the river Meuse on the Belgium-Dutch border. Evolution of clutches has been followed during two breeding seasons (1991 and 1992). The features of the nests and the nest-sites have been subjected to a multivariate analysis. It’s difficult to distinguish clearly between successful and unsuccessful nests during the same year. Moreover, most characteristics are not constant any year. Only two parameters seem to have the same effects on nesting success in 1991 and 1992 : the distance from nest to nearest neighbour and the water level in nest site. Nests distant from another nest and in deep water were less successful. Other possible causes of nesting failure have been tackled (investigator disturbance, bad weather conditions, fall of eggs, clutch-destruction by human). [less ▲]

Detailed reference viewed: 25 (5 ULg)
Peer Reviewed
See detailMachine Intelligent Automatic Recognition of Critical Mobile Targets in Laser Radar Imagery
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

in Lincoln Laboratory Journal (1993), 6(1), 161-186

Detailed reference viewed: 14 (4 ULg)
Full Text
See detailFouilles paléolithiques 1992 en Belgique
Otte, Marcel ULg; Straus, Lawrence; Gautier, Achilles et al

in Notae Praehistoricae (1993), 12

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailControl of the illegal administration of natural steroid hormones in urine and tissues of veal calves and in plasma of bulls
Scippo, Marie-Louise ULg; Gaspar, Pol; Degand, Guy ULg et al

in Analytica Chimica Acta (1993), 275(1-2), 57-74

Detailed reference viewed: 10 (3 ULg)
Peer Reviewed
See detailThree-dimensional General Circulation Model of the Northern Bering Sea’s Summer Ecohydrodynamics
Nihoul, Jacques ULg; Adam, Paul; Brasseur, Pierre et al

in Continental Shelf Research (1993), 13(5-6), 509-542

The main features of the northern Bering Sea's summer ecohydrodynamics are investigated with the help of two three-dimensional—direct and inverse—models developed at theGeoHydrodynamics ... [more ▼]

The main features of the northern Bering Sea's summer ecohydrodynamics are investigated with the help of two three-dimensional—direct and inverse—models developed at theGeoHydrodynamics andEnvironmentResearch Laboratory of the University of Liège (GHER). Each model consists of two interacting sectorial submodels for (i) the general circulation hydrodynamics and synoptic structures, and (ii) the associated plankton ecosystem dynamics. The direct model is used to simulate, from an initial state compatible with historical, climatological and all available data pertinent to the summer season, a typical overview of the northern Bering Sea's ecohydrodynamics during the summer. The inverse model is applied in a two-fold perspective: (i) the reconstruction of typical summer distributions of temperature and salinity by using more than 1500 CTD profiles measured during the months of July, August and September, in the course of the ISHTAR program; (ii) considering the observations from specific ISHTAR surveys as quasi-synoptic, the reconstruction of individual data fields in order to provide additional information to assess the variability of the system. The model's predictions indicate that the summer dynamics are dominated by a few cogent semi-permanent and reproducible mechanisms which govern the main water mass transports, the upwellings, the fronts and the subsequent seasonal patterns of primary and secondary productions. The general circulation fields calculated by the direct model are considered as a standard of reference to give a coherent interpretation of—local and often instantaneous—observations, process studies and related results, in the context of the natural variability of the system. The simulated flow pattern has been validated, using the set of current measurements provided by 1985 and 1986 ISHTAR moorings. The contribution of the Anadyr Stream to the northward transport is reproduced qualitatively and quantitatively. The vertical motions—undetectable from direct experiments—are computed by the model, and represent one of the most efficient constraints on the ecohydrodynamics. For instance, the strong upwelling located along the Siberian coast—the existence of which was only presumed until recently—is now correctly estimated in position and intensity. The exceptionally high concentrations of nutrients found in the upwelled water turn this hydrodynamic structure into a catalyst element for the development of biological species in the region. The pattern of primary production shows two successive maxima: the first appears as a direct consequence of the frontal conditions associated with the Anadyr Stream, whereas the second develops further north, in the Chukchi Sea. The results display a fairly good agreement with the classical descriptions induced from observations, and suggest that the advection-growth coupling is the main physical conditioning factor for biological processes. [less ▲]

Detailed reference viewed: 8 (2 ULg)
Peer Reviewed
See detailLa catalyse enzymatique en milieu organique : potentialités d'utilisation des lipases.
Blecker, Christophe ULg

in Bulletin des Recherches agronomiques de Gembloux (1993), 28(1), 51-85

Detailed reference viewed: 40 (6 ULg)
Peer Reviewed
See detailVisualizing the evolution of a star on a graphic workstation
Noels-Grötsch, Arlette ULg; Papy, Roland; Remy, F.

in Computers in Physics (1993), 7(2), 202-207

Theoretical models of a one solar mass star from the gravitational contraction onto the main sequence of the Hertzsprung-Russell diagram are visualized on a Tektronix 4337 workstation.

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailCytokine and anti-cytokine strategies in inflammatory reaction modulation
Delannoy, I.; Lekeux, Pierre ULg; Miossec, P.

in Veterinary Research (1993), 24

Evidence of the importance of a cytokine cascade in the induction and control of the inflammatory reaction is increasing. Although cytokines are required in the inflammatory process in response to ... [more ▼]

Evidence of the importance of a cytokine cascade in the induction and control of the inflammatory reaction is increasing. Although cytokines are required in the inflammatory process in response to infection or injury, their overproduction, particularly that of interleukin-1 (IL-1) and tumor necrosis factor (TNF), can lead to local/systemic pathology. Selective inhibition of the synthesis or of the action of specific cytokines may be of therapeutic benefit. Various strategies for blocking IL-1 and TNF activities have been presented. While selective inhibition of cytokine synthesis is still in the early experimental phase, specific blockade of soluble cytokine action following synthesis and release from cells is undergoing preliminary clinical trials in humans. Animal data suggest that antimonoclonal therapy such as monoclonal antibodies to TNF, IL-1 receptor antagonists or soluble receptors to TNF and IL-1 can be effective in the modulation of the inflammatory reaction. Modulation of the cytokine network in some diseases might also include the use of anti-inflammatory cytokines. Nonetheless, the possibilities of side effects due to impaired host-defense mechanisms with the IL-1 or TNF blockade must also be taken into consideration. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailLa place de la radiothérapie dans le traitement des sarcomes des tissus mous
Coucke, Philippe ULg; Guillemin, C-L

in Bulletin du Cancer. Radiothérapie : Journal de la Société Française du Cancer : Organe de la Société Française de Radiothérapie Oncologique (1993), 80(1), 13-26

La radiothérapie des sarcomes des tissus mous est revue à travers la littérature. Comme la chirurgie, le traitement actinique fait partie du traitement standard. Comparée à la radiothérapie postopératoire ... [more ▼]

La radiothérapie des sarcomes des tissus mous est revue à travers la littérature. Comme la chirurgie, le traitement actinique fait partie du traitement standard. Comparée à la radiothérapie postopératoire, l'approche préopératoire et/ou interstitielle offre l'avantage potentiel de nécessiter de façon plus systématique une approche multidisciplinaire ab initio. Par ailleurs, la radiothérapie préopératoire implique une irradiation des tissus sains moins importante et donc une réduction probable de la toxicité. Le contrôle local et la survie sont comparables pour les deux modalités radiothérapeutiques. Toutefois, la comparaison entre différentes séries reste ardue vu l'hétérogénéité des populations étudiées. Une solution serait d'effectuer une étude randomisée [less ▲]

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailCloning of a human GHF-1/Pit-1 cDNA variant
Pernasetti, Flavia; Wera, Stefaan; Belayew, Alexandra et al

in Nucleic Acids Research (1993), 21(15), 3584

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailLa cinétique cellulaire tumorale: aspects cliniques «à la recherche du temps perdu»
Coucke, Philippe ULg; Paschoud, N

in Bulletin du Cancer. Radiothérapie : Journal de la Société Française du Cancer : Organe de la Société Française de Radiothérapie Oncologique (1993), 80(4), 431-437

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailAérosolthérapie chez le cheval : 2. Etude pharmacologique
Lekeux, Pierre ULg; Genicot, Bruno; Votion, Dominique ULg et al

in Pratique Vétérinaire Equine (1993), 25(4), 233-243

This review analyzes the different therapeutic drugs the functional effects of which can be useful in the treatment by aerosol of equine respiratory disorders

Detailed reference viewed: 40 (2 ULg)
Peer Reviewed
See detailStructure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution
Noble, M. E.; Zeelen, J. P.; Wierenga, R. K. et al

in Acta Crystallographica Section D-Biological Crystallography (1993), 49(Pt 4), 403-17

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 ... [more ▼]

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 A resolution with a crystal grown by the technique of hanging-drop crystallization from a mother liquor containing the transition-state analogue 2-phosphoglycolate (2PG). As a search model in the molecular replacement calculations, the refined structure of TIM from Trypanosoma brucei, which has a sequence identity of 46% compared to the enzyme from E. coli, was used. An E. coli TIM crystal grown in the absence of 2PG, diffracting to 2.6 A resolution, was later obtained by application of the technique of macro-seeding using a seed crystal grown from a mother liquor without 2PG. The final 2.6 A model has a crystallographic R factor of 11.9%, and agrees well with standard stereochemical parameters. The structure of E. coli TIM suggests the importance of residues which favour helix initiation for the formation of the TIM fold. In addition, TIM from E. coli shows peculiarities in its dimer interface, and in the packing of core residues within the beta-barrel. [less ▲]

Detailed reference viewed: 7 (1 ULg)
Full Text
See detailEvolution de la situation des mammifères sauvages en Région wallonne au cours de décennie 1983-1992
Libois, Roland ULg

in Cahiers d'Ethologie (1993), 13(1), 77-92

During the last ten years, the evolution of the status of the mammal fauna of Wallony (southern part of Belgium) can be characterized by four main trends: i- enforcement of a better legal protection ii ... [more ▼]

During the last ten years, the evolution of the status of the mammal fauna of Wallony (southern part of Belgium) can be characterized by four main trends: i- enforcement of a better legal protection ii- arrival of at least four new species: coypu, raccoon, raccon-dog and American mink iii- restoration of badger numbers following the efficient control of rabies iv- increase of anthropophilic or game species (stone marten, ungulates). Unfortunately, the most threathened species (otter, horseshoe bats, barbastella) did not recover. [less ▲]

Detailed reference viewed: 29 (5 ULg)
Full Text
Peer Reviewed
See detailEssential oil production by Anthemis nobilis L. tissue culture.
Fauconnier, Marie-Laure ULg; Jaziri, M.; Marlier, Michel et al

in Journal of Plant Physiology (1993), 141(6), 759-761

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was ... [more ▼]

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was compared with that of plants grown in a field. The addition of crude polysaccharide fraction prepared from yeast extract and from the plant itself to shoot cultures affected the composition and total essential content (from 0.08 o/o to 0.3Qo/o dry weight). The essential oil content of the crown-gall tissue ol A. nobilis was 0.25 % of the dry weight and the composition of the essential oil was comparable to that of the flowers. [less ▲]

Detailed reference viewed: 186 (4 ULg)
Peer Reviewed
See detailInfections par streptocoque B en période néonatale. Epidémiologie et prévention
Battisti, Oreste ULg

in Archives Françaises de Pédiatrie (1993), 50

Detailed reference viewed: 10 (3 ULg)
See detailRapport préliminaire sur la 2e campagne de fouille à Tell Amarna (Syrie)
Tunca, Önhan ULg

in Akkadica (1993), 83

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailThe role of GnRH agonists in the endoscopic treatment of endometriosis and fibromyomas.
Donnez, Jacques; NISOLLE, Michelle ULg; Grandjean, P. et al

in Contraception, Fertilite, Sexualité (1992) (1993), 21(1), 59-62

Uterine myomas and endometriosis are benign pathologies frequently encountered in women. Myomas are often associated with infertility and/or menorrhagia particularly if they are sub-mucosal. Endometriosis ... [more ▼]

Uterine myomas and endometriosis are benign pathologies frequently encountered in women. Myomas are often associated with infertility and/or menorrhagia particularly if they are sub-mucosal. Endometriosis is diagnosed in more than 35% of infertile patients. These two common pathologies are oestrogen-dependent and the administration of a GnRH agonist has been proposed as a non-surgical approach to the treatment of myomas and endometriosis. GnRH agonists cannot, however, be considered as definitive medical therapy because most myomas and endometriotic cysts return to their initial size within 4 months following the cessation of treatment. Moreover, because of the menopausal-like state that they induce, GnRH agonists provoke bone demineralization and for this reason, their long-term use is not recommended. These agents should, therefore, be considered as an adjuvant preoperative therapy. The aim is, above all, to achieve a preoperative reduction of tumour size, thus facilitating the endoscopic surgery: either hysteroscopic resection in the case of sub-mucosal myomas, or vaporization of ovarian cysts in the case of cystic endometriotic lesions. [less ▲]

Detailed reference viewed: 26 (0 ULg)
See detailViolences des jeunes : à l'école et ailleurs ?
Born, Michel ULg; Gavray, Claire ULg

in Observatoire : Revue d'Action Sociale & Médico-Sociale (1993), 6

Detailed reference viewed: 30 (6 ULg)
See detailHet Craeckhuys van de Bijloke in Gent, dendrochronologische analyse van houtmonsters
Hoffsummer, Patrick ULg; Houbrechts, David

in Stadsarcheologie (1993), 17(4), 24-29

Detailed reference viewed: 48 (1 ULg)
See detailLa racine grb et l'Amon-grb
Malaise, Michel ULg; Winand, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1993), 68

Detailed reference viewed: 44 (1 ULg)
Full Text
Peer Reviewed
See detailComparison of the cardiac pumping capability and cardiac pumping reserve in double muscled and conventional calves
Amory, Hélène ULg; Mc Entee, Kathleen ULg; Linden, Annick ULg et al

in Canadian Journal of Physiology & Pharmacology (1993), 71(12), 946-951

Hereditary muscular hypertrophy is a character that has been selected in several animal species for industrial meat production. The selection of this character in cattle produces animals of exceptional ... [more ▼]

Hereditary muscular hypertrophy is a character that has been selected in several animal species for industrial meat production. The selection of this character in cattle produces animals of exceptional commercial value but ones with a lower aerobic capacity than that of conventional cattle. The purpose of this work was to study the role of cardiac function as a potential limiting factor of aerobic capacity in double-muscled calves. Two groups of healthy calves were studied, one consisting of nine calves of conventional conformation and the other of nine double-muscled calves. Pulmonary arterial and capillary wedge, central venous, and systemic arterial pressures were measured by fluid-filled catheters and recorded together with the electrocardiogram. Cardiac output was measured by the thermodilution technique. From these measurements, the heart rate, the cardiac and the stroke indices, the pulmonary and the systemic vascular resistances, and the cardiac power output were calculated. The parameters were recorded under basal resting conditions and during incremental dobutamine challenge, which allowed determination of the resting cardiac power output, the cardiac pumping capability, and the cardiac reserve. Dobutamine challenge induced a significant rise in cardiac and stroke indices, heart rate, and cardiac power output, a significant decrease in pulmonary and systemic vascular resistances, and no change in systemic and pulmonary arterial pressures. The cardiac reserve obtained in the present study was low in comparison with those previously reported in humans, dogs, and horses. This may be related to the poor running capability of bovine species relative to that of the former species.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailHistoplasmose africiaine à Histoplasma duboisii (Histoplasma capsulatum var. duboisii): quatorze cas congolais observés en 10 ans (1981-1990)
Carme, Bernard; Hayette, Marie-Pierre ULg; Itoua Ngaporo, A. et al

in Journal de Mycologie Médicale (1993), 3

Fourteen cases of histoplasmosis due to Histoplasma duboisii seen in Congo from 1981 through 1990, are reported: the average age of the patients was 25 years (the youngest being 1,5 y and the oldest 50y ... [more ▼]

Fourteen cases of histoplasmosis due to Histoplasma duboisii seen in Congo from 1981 through 1990, are reported: the average age of the patients was 25 years (the youngest being 1,5 y and the oldest 50y), the M/F ratio was 2,5; most of the patients (10/14) live in a rural area. there was a rather constant frequency of one to three cases per annum from 1981 through 1990. The fisrt congolese AIDS-associated systemic histoplasmosis duboisii case was observed in 1990. Lymph node involvment and mucocutaneous lesions were the most frequent clinical manifestations. In all cases, the disgnosis was established by histological examination. When performed, direct microscopy was always positive (11/11). Isolation of H. duboisii in culture was successful in 7 out of 9 attempts. Two cases of systemic infection were rapidly fatal but a good clinical response was obtained in the other cases wih amphotericin B, which due to intolerance, was replaced by ketoconazole in 2 patients. [less ▲]

Detailed reference viewed: 25 (2 ULg)
Full Text
Peer Reviewed
See detailHypofractionation in retinoblastoma: an increased risk of retinopathy.
Coucke, Philippe ULg; Mirimanoff, R-O; Schmid, C et al

in Radiotherapy & Oncology (1993), 28

Forty-four eyes in 38 children were treated between 1963 and 1991 by external radiotherapy for retinoblastoma. Treatment modalities varied widely during this period; in addition to radiotherapy there was ... [more ▼]

Forty-four eyes in 38 children were treated between 1963 and 1991 by external radiotherapy for retinoblastoma. Treatment modalities varied widely during this period; in addition to radiotherapy there was chemotherapy (16/44), photocoagulation (14/44), and laser therapy or cryotherapy (14/44). Treatment technique and dose fractionation also varied widely; lateral beam technique (39/44) versus anterior or anterior/lateral beam; doses per fraction ranged from 1 to 4.5 Gy, total doses from 30 to 61.5 Gy, and overall times from 22 to 49 days. Patients were followed at 3-month intervals, and actuarial survival at 10 years was 88%, with 62% local control. Ten eyes showed clinical evidence of retinopathy. A multivariate analysis of factors associated with increased risk of retinopathy was carried out using the Cox proportional hazards model and the mixture model of Farewell. The estimated latent time was 17 months (95% confidence interval, 14-20 months). The only factors found to be significantly associated with retinopathy were total dose multiplied by dose per fraction, or total dose normalized to the equivalent total dose in 2-Gy fractions as estimated from the LQ model, and these gave equivalent descriptions. There were trends (not significant) for increased risk of retinopathy when treatments included chemotherapy or photocoagulation, and for decreased risk (also not significant) when cryotherapy was used in conjunction with radiotherapy. No significance could be attached to any of the following: number of sites per eye, Reese-Ellsworth stage, and family history. We conclude that hypofractionation carries a significant risk for retinopathy in the treatment of retinoblastoma. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailFRICTIONAL CONTACT FINITE-ELEMENTS BASED ON MIXED VARIATIONAL-PRINCIPLES
Cescotto, Serge ULg; Charlier, Robert ULg

in International Journal for Numerical Methods in Engineering (1993), 36(10), 1681-

This paper presents an original approach to the numerical modelling of unilateral contact by the finite element method. The main point is the development of mixed contact finite elements in which the ... [more ▼]

This paper presents an original approach to the numerical modelling of unilateral contact by the finite element method. The main point is the development of mixed contact finite elements in which the displacement field and the contact stress field (pressure and friction shear) are discretized independently. The theory, based on variational principles, is first presented in the framework of infinitesimal deformations and, subsequently, is extended to large inelastic strains. [less ▲]

Detailed reference viewed: 80 (23 ULg)
Peer Reviewed
See detailBeam-laser lifetime measurements for low-lying levels in Cr II
Pinnington, E. H.; Ji, Q.; Guo, B. et al

in Canadian Journal of Physics (1993), 71

Detailed reference viewed: 3 (2 ULg)
Peer Reviewed
See detailGLUCOCORTICOIDS AS DOPING AGENTS IN HOMING PIGEONS
Duchatel, Jean-Pierre ULg; Beduin, Jean-Marie ULg; Jauniaux, Thierry ULg et al

in Annales de Médecine Vétérinaire (1993), 137(8), 557-564

Different commercially available glucocorticoids that could be used to dope racing pigeons have been evaluated using zootechnical and biological parameters. The influence of these corticosteroids on blood ... [more ▼]

Different commercially available glucocorticoids that could be used to dope racing pigeons have been evaluated using zootechnical and biological parameters. The influence of these corticosteroids on blood values have been investigated. Toxicity of triamcinolone diacetate varied proportionally to injected doses and the main effects observed were lymphoid tissue alterations, muscular atrophy with breast muscles fibers vacuolation, fatty degeneration of liver and kidneys, hypoplasia or atrophy of gonads, and, in the blood, a significant increase of calcium and triglycerides levels. Prednisolone acetate in ocular drops was less toxic than dexemathasone and fluocinolone acetonide. The type of corticoids also had an influence on hemoglobin, glucose, and creatinine levels. The inhibition of the molt was also observed and varied with the sort of product used. [less ▲]

Detailed reference viewed: 151 (1 ULg)
Peer Reviewed
See detailIn Vivo Free Radical Production after Cross-Clamping and Reperfusion of the Renal Artery in the Rabbit
DEFRAIGNE, Jean ULg; PINCEMAIL, Joël ULg; FRANSSEN, Colette ULg et al

in Cardiovascular Surgery (1993), 1(4), 343-9

Postischaemic reperfusion injury is often attributed to the generation of oxygenated free radicals which may subsequently promote lipid peroxidation in cell membranes. Electron paramagnetic resonance ... [more ▼]

Postischaemic reperfusion injury is often attributed to the generation of oxygenated free radicals which may subsequently promote lipid peroxidation in cell membranes. Electron paramagnetic resonance spectroscopy in association with the spin trap molecule alpha-phenyl-N-tert-butyl-nitrone allowed direct confirmation of lipid free radical production after renal ischaemia-reperfusion in an in vivo rabbit model. A 60-min period of ischaemia followed by reperfusion caused free radical production twofold greater than after 15 min of ischaemia. Glutathione and alpha-tocopherol have been measured in renal tissue, as indirect markers of lipid peroxidation. After 15 min of ischaemia followed by 10 min of reperfusion, the mean(s.e.m.) glutathione content of the ischaemic kidney was slightly but significantly reduced by 11.9(2.5)% (P < 0.003). The content of alpha-tocopherol was unchanged. However, 10 min of reperfusion following 60 min of ischaemia led to significant decrease in mean(s.e.m.) content of both glutathione (30.4(3.7)%) (2.23(0.2) versus 3.14(0.18) mumol/g wet tissue, P < 0.001) and alpha-tocopherol (46.1(7.8)%) (0.57(0.10) versus 1.09(0.14) micrograms/g wet tissue, P < 0.001) when compared to the control kidney. Under these experimental conditions, desferrioxamine (15 mg/kg administered intravenously before inducing ischaemia), a drug known to limit free radical production, significantly limited the decrease of alpha-tocopherol to 20.8(6.4)% (0.83(0.08) versus 1.05(0.04) micrograms/g wet tissue, P < 0.05), but did not prevent glutathione consumption in the reperfused kidney. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailEffects of a 1-year treatment with a low-dose combined oral contraceptive containing ethinyl estradiol and cyproterone acetate on glucose and insulin metabolism.
Scheen, André ULg; Jandrain, Bernard ULg; Humblet, Dominique ULg et al

in Fertility and Sterility (1993), 59(4), 797-802

OBJECTIVE: To study the effects of the slightly estrogen-dominant monophasic low-dose oral contraceptive (OC) Diane-35 (Schering AG, Berlin, Germany) (35 micrograms ethinyl estradiol [EE2] + 2 mg ... [more ▼]

OBJECTIVE: To study the effects of the slightly estrogen-dominant monophasic low-dose oral contraceptive (OC) Diane-35 (Schering AG, Berlin, Germany) (35 micrograms ethinyl estradiol [EE2] + 2 mg cyproterone acetate, a 17 alpha-hydroxyprogesterone derivative [17-OHP]) on glucose and insulin metabolism. DESIGN: Seven healthy young women were investigated by using the euglycemic hyperinsulinemic glucose clamp technique (insulin delivery rate = 100 mU/kg per hour for 120 minutes). This test was performed, after an overnight fast, during the last 7 days of a spontaneous cycle and within the last 5 days of pill intake during the sixth and twelfth cycle of a continuous treatment with Diane-35 in each subject. RESULTS: The three indexes measuring the insulin-induced glucose disposal during the clamp (glucose infusion rate, glucose metabolic clearance rate, and glucose infusion rate divided by plasma insulin plateau levels) were not significantly affected by Diane-35. In contrast, the metabolic clearance rate of the exogenous insulin infused during the clamp tended to be slightly increased with Diane-35 (significant after 6 but not after 12 cycles). CONCLUSION: These results suggest that a 1-year treatment with the OC Diane-35, which contains EE2 + a 17-OHP rather than a 19-nortestosterone derivative as the progestogen compound, does not significantly alter peripheral (presumably muscular) insulin sensitivity but slightly increases insulin (presumably hepatic) clearance. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Peer Reviewed
See detailVisite du Trésor de la Cathédrale de Liège
George, Philippe ULg

in Feuillets de la Cathédrale de Liège (1993)

Detailed reference viewed: 12 (1 ULg)
Peer Reviewed
See detailEngery-filtering transmission electron microscopy of biological specimens.
de Bruijn, W. C.; Sorber, C. W.; Gelsema, E. S. et al

in Scanning Microscopy (1993), 7(2), 693-708709

By energy-filtering transmission electron microscopy (EFTEM) electrons can be separated by their energy losses. An electron-energy filter, added to the microscope column allows the measurement of the ... [more ▼]

By energy-filtering transmission electron microscopy (EFTEM) electrons can be separated by their energy losses. An electron-energy filter, added to the microscope column allows the measurement of the energy distribution of transmitted electrons that have lost energy (< 2,000 eV, with an energy resolution of approximately 1 eV). These filtered electrons, recorded either as a spectrum or as an image, are composed of two parts superimposed on top of each other: (a) the unspecific energy-loss population (= the continuum) and (b) the specific element-related energy-loss population (= the edges). At the edges, electron data in spectra and images are mathematically processed, to obtain the desired element-related net-intensity values or images. These data are related to the total transmitted electron intensity, from the zero- and low-loss spectral region giving the relative spectralor image intensity rations ((S)R*x, (I)R*x), which can be related to the element concentration. The acquisition of the zero-loss and low-loss data is hampered by the restricted dynamic range of the TV camera. By improvements through the introduction of calibrated attenuation filters in the optical path to the TV-camera, more reliable values for (S)R*x and (I)R*x can be acquired. By addition of Bio-standards adjacent to the tissue, a "known" and "unknown" concentration of the element present in the same ultrathin section and the "bias" in the concentration estimation, can be obtained. Some practical examples are given for the estimation of the iron cencentration in siderosomes, boron in melasosomes and calcium in calcium oxalate monohydrate crystals. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailExemples d'utilisation de la Carte des Sols de Belgique comme base d'un systeme d'information geographique pour la gestion des terroirs.
Engels, Patrick ULg; Bock, Laurent ULg; Neven, Cécile et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(2-3),

Detailed reference viewed: 47 (6 ULg)
See detailDu bon steak et du bon lait: d'abord interroger le naturel et en percer tous ces secrets
Renaville, Robert ULg; Burny, Arsène; Portetelle, Daniel ULg

in Agricontact (1993), 255

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailAn overview of the kinetic parameters of class B beta-lactamases.
Felici, A; Amicosante, G; Oratore, A et al

in Biochemical Journal (1993), 291 ( Pt 1)

The catalytic properties of three class B beta-lactamases (from Pseudomonas maltophilia, Aeromonas hydrophila and Bacillus cereus) were studied and compared with those of the Bacteroides fragilis enzyme ... [more ▼]

The catalytic properties of three class B beta-lactamases (from Pseudomonas maltophilia, Aeromonas hydrophila and Bacillus cereus) were studied and compared with those of the Bacteroides fragilis enzyme. The A. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. No relationships were found between sequence similarities and catalytic properties. The problem of the repartition of class B beta-lactamases into sub-classes is discussed. Improved purification methods were devised for the P. maltophilia and A. hydrophila beta-lactamases including, for the latter enzyme, a very efficient affinity chromatography step on a Zn(2+)-chelate column. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Peer Reviewed
See detailIdentification of elemental sulphur (S8) in Boscia senegalensis (Pers.) Lam ex Poir. leaves.
Lognay, Georges ULg; Seck, D.; Marlier, M. et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(4),

Detailed reference viewed: 22 (4 ULg)
Peer Reviewed
See detailCharacterization of somatostatin transactivating factor-1, a novel homeobox factor that stimulates somatostatin expression in pancreatic islet cells.
Peers, Bernard ULg; Johnson, T.; Ferreri, K. et al

in Molecular Endocrinology (1993), 7(10), 1275-83

The endocrine pancreas consists of several differentiated cell types that are distinguished by their selective expression of peptide hormones such as insulin, glucagon, and somatostatin. Although a number ... [more ▼]

The endocrine pancreas consists of several differentiated cell types that are distinguished by their selective expression of peptide hormones such as insulin, glucagon, and somatostatin. Although a number of homeobox-type factors have been proposed as key regulators of individual peptide genes in the pancreas, their cellular distribution and relative abundance remain uncharacterized. Also, their overlapping DNA binding specificities have further obscured the regulatory functions these factors perform during development. In this report we characterize a novel homeobox-type somatostatin transactivating factor termed STF-1, which is uniformly expressed in cells of the endocrine pancreas and small intestine. The 283-amino acid STF-1 protein binds to tissue-specific elements within the somatostatin promoter and stimulates somatostatin gene expression both in vivo and in vitro. Remarkably, STF-1 comprises the predominant tissue-specific element-binding activity in nuclear extracts from somatostatin-producing pancreatic islet cells, suggesting that this protein may have a primary role in regulating peptide hormone expression and specifying endocrine cell lineage in the developing gut. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailMeasurement of Total Respiratory Impedance in Dogs by the Forced Oscillation Technique
Clercx, Cécile ULg; Gustin, Pascal ULg; Landser, F. J. et al

in Veterinary Research Communications (1993), 17(3), 227-239

The resistance (Rrs) and reactance (Xrs) of the total respiratory system were determined at various frequencies in 14 healthy conscious beagle dogs. A pseudorandom noise pressure wave was produced at the ... [more ▼]

The resistance (Rrs) and reactance (Xrs) of the total respiratory system were determined at various frequencies in 14 healthy conscious beagle dogs. A pseudorandom noise pressure wave was produced at the nostrils of the animals by means of a loudspeaker adapted to the nose by a tightly fitting mask. A Fourier analysis of the pressure and flow signals yielded mean Rrs and Xrs, over 16 s, at frequencies from 2 to 26 Hz. The influence of the posture of the dog, the position of its head, the linearity of the respiratory system, the reproducibility of the method and the effects of upper and lower airway obstructions were studied. In sitting and standing healthy dogs with the head in the extended position, Rrs values increased progressively with frequency from 5.4 +/- 0.4 (SEM) cmH2O L-1s at 6 Hz up to 8.8 +/- 0.7 cmH2O L-1s at 26 Hz, the mean resonant frequency being 6.1 +/- 0.5 Hz. No significant differences were observed between measurements performed with the head in the normal or the extended position. In a recumbent posture, all Rrs values were increased but Rrs was still dependent on the frequency in the same way (7.1 +/- 0.7 cmH2O L-1s at 6Hz up to 10.0 +/- 0.5 cmH2O L-1s at 26 Hz). Tracheal compression also induced higher Rrs values without changes in the frequency dependence or in the resonant frequency. In anaesthetized dogs, airway obstruction was induced by inhalation of histamine (4 mg/ml for 5 min; the Rrs values tended to decrease with increasing frequency, and the resonant frequency was markedly increased [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailProduction of active oxygen species by isolated human chondrocytes.
Henrotin, Yves ULg; Deby-Dupont, G.; DEBY, C. et al

in British Journal of Rheumatology (1993), 32(7), 562-7

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric ... [more ▼]

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric method (production of dichlorofluorescein from a precursor in the presence of horseradish peroxidase and H2O2) and by a chromatographic method (measurement of ethylene production from gamma-methiol-keto-butyric acid after .OH attack). Chondrocytes were tested, both with and without activation by phorbol myristate acetate (PMA: 10(-6) M), in the presence of Ca2+ (1 x 10(-4) M) and Mg2+ (2 x 10(-4) M) or after variable periods of anoxia under nitrogen (4 to 12 h) followed by reoxygenation (with 95% O2, 5% CO2). Under these experimental conditions, the PMA-excited chondrocytes produced from 80 to 180 nmol of hydrogen peroxide per 1 x 10(6) cells and chondrocytes subjected to anoxia-reoxygenation produced up to 1700 nmol H2O2 per 1 x 10(6) cells. The hydroxyl radical production by PMA or anoxia-reoxygenation excited cells reached 600% of the production of non-excited cells and 1300% when they were subjected to successive stimulations by PMA and anoxia-reoxygenation. The possible pathological significance of these observations is discussed. The results indicate that stimulated human chondrocytes are capable of producing active oxygen species which could play a major role in joint inflammation and cartilage damage. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailHEXAGONAL MARANGONI CONVECTION IN A RECTANGULAR BOX WITH SLIPPERY WALLS
Dauby, Pierre ULg; Lebon, Georgy ULg; COLINET, P. et al

in Quarterly Journal Of Mechanics And Applied Mathematics (1993), 46(Part 4), 683-707

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailNew f values in C I and the CNO Abundances in the Sun
Biémont, Emile ULg; Hibbert, A.; Godefroid, M. et al

in Astrophysical Journal (1993), 412

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailPurification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
Feron, C.; Gothot, André ULg; Grosdent, Jean-Claude ULg et al

in Journal of Hematotherapy (1993), 2(2), 259-61

CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a ... [more ▼]

CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells. [less ▲]

Detailed reference viewed: 24 (1 ULg)
Peer Reviewed
See detailIntracellular damages induced by singlet oxygen are signals for HIV-1 reactivation
Piette, Jacques ULg; Legrand-Poels, Sylvie ULg

in Journal of Free Radicals in Biology & Medicine (1993)

Detailed reference viewed: 6 (1 ULg)
Peer Reviewed
See detailEffect of androgen treatmen on fear reactions in ewes
Vandenheede, Marc ULg; Bouissou, M. F.

in Hormones & Behavior (1993), 27

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailSynthesis and Inverse Emulsion Polymerization of Aminated Acrylamidodextran
Daubresse, Catherine; Grandfils, Christian ULg; Jérôme, Robert ULg et al

in Journal of Pharmacy & Pharmacology (1993), 45

A chemically modified form of dextran was prepared; having a polymerizable moiety (acrylamide) and a reactive functional group (primary amine). Dextran was activated with 4-nitrophenylchloroformate (24 ... [more ▼]

A chemically modified form of dextran was prepared; having a polymerizable moiety (acrylamide) and a reactive functional group (primary amine). Dextran was activated with 4-nitrophenylchloroformate (24 mol per polysaccharide, 9,8 mol per 100 glucose residues); 9,8% glucose residues were converted to aliphatic carbonates and 5,2% were converted to cyclic carbonates. The activated dextran was coupled with trityldiaminoethane (8 mol per 100 glucose residues), reactivated with 4-nitrophenylchloroformate, then coupled with acryloamidodiaminohexane (6,8 mol per 100 glucose residues). The trityl group was removed by hydrolysis with trifluoroacetic acid to yield the required aminated acryloamidodextran. The modified dextran was shown to be polymerizable by inverse emulsion polymerization. Submicron particules were successfully prepared, containing functional amine groups suitable for preparing drug conjugates or for modifying the surface properties of thisbiomaterial. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailDecision tree approach to power systems security assessment
Wehenkel, Louis ULg; Pavella, Mania ULg

in International Journal of Electric Power and Energy Systems (1993), 15(1), 13-36

An overview of the general decision tree approach to power system security assessment is presented. The general decision tree methodology is outlined, modifications proposed in the context of transient ... [more ▼]

An overview of the general decision tree approach to power system security assessment is presented. The general decision tree methodology is outlined, modifications proposed in the context of transient stability assessment are embedded, and further refinements are considered. The approach is then suitably tailored to handle other specifics of power systems security, relating to both preventive and emergency voltage control, in addition to transient stability. Trees are accordingly built in these various application domains, and their salient features are explored, assessed, compared. Among attractive aspects of the trees, we mention their ability to uncover the intrinsic mechanism governing physical processes, and to provide a clear description in terms of tractable system parameters. Further, their dual, 'attribute space' representation is shown to have complementary possibilities for more refined analysis and, in addition, for sensitivity assessment and control. Overall, the approach is characterized by its great flexibility with respect to tree structure, types of physical parameters driving the phenomena, and classes of potential applications. To illustrate and support the developments,real-world examples are reported, rolatin 9 to transient and voltage stability issues simulated on several power systems. [less ▲]

Detailed reference viewed: 36 (4 ULg)
Full Text
Peer Reviewed
See detailApplications de l'échographie en reproduction bovine. 2. L'utérus gestant et non-gestant.
Hanzen, Christian ULg; Laurent, Yves; Jakovljevic, Samy

in Annales de Médecine Vétérinaire (1993)

Detailed reference viewed: 56 (6 ULg)
Peer Reviewed
See detailEffects of Age and Indomethacin on Response and Sensitivity of Pulmonary Artery to Phenylephrine and to Histamine in Pigs
Gustin, Pascal ULg; Ansay, Michel; Advenier, C.

in Journal of Veterinary Pharmacology & Therapeutics (1993), 16(2), 207-213

The vasoconstrictor effects of phenylephrine and histamine were investigated in isolated strips of pulmonary arteries in pigs during ageing. Interactions between phenylephrine-induced responses and ... [more ▼]

The vasoconstrictor effects of phenylephrine and histamine were investigated in isolated strips of pulmonary arteries in pigs during ageing. Interactions between phenylephrine-induced responses and arachidonic acid derivatives were also studied by incubating the blood-vessels with indomethacin. Potency (pD2 values) and maximal effects (Emaxx) recorded in 5-week-old piglets (group I, n= 5) with phenylephrine [5.71 ± 0.17 and 0.76 ± 0.22 g/mg of dry tissue respectively (mean ± SEM)] were similar to values found in 12-week-old animals (group 2, n = 5) (5.49 ± 0.30 and 1.06 ± 0.27 g/mg of dry tissue respectively). The sensitivity and responsiveness of tissues to this agonist were significantly reduced in 26-week-old mature pigs (group 3, n = 6) as indicated by the decrease in pD2 (3.91 ± 0.23; P < 0.01) and Emax (0.27 ± 0.13 g/mg of dry tissue; P < 0.05) values observed in this group. Histamine (10_3M)-induced maximal responses (Emax) were significantly higher in group 2 (2.23 ± 0.49 g/mg) than in group 1 (0.85 ± 0.11 g/mg; P < 0.05) and in group 3 (0.48 ± 0.10 g/mg; P < 0.01). In 5-week-old animals, indomethacin (3.10˜5M) significantly (P < 0.05) shifted the concentration-response curve to phenylephrine to the right (0.28 log. units) and depressed contractions to this drug as shown by the significant decrease of 39.5% (P < 0.05) in Emax. This cyclo-oxygenase inhibitor had no effect in other groups. These data indicate that phenylephrine is a potent and effective vasoconstrictor agent for the main pulmonary arteries in 5-week-old piglets and that alpha-1-adrenergic-induced contractions are enhanced by cyclo-oxygenase products. These findings can be related with the high reactivity of pulmonary vascular smooth muscles in these animals [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailPiroxicam-Beta-Cyclodextrin in the Treatment of Acute Pain of Rheumatic Disease
Reginster, Jean-Yves ULg; Franchimont, P.

in European Journal of Rheumatology and Inflammation (1993), 12(4), 38-46

Analgesics continue to be the mainstay of therapy in osteoarthritis. Non-steroidal anti-inflammatory drugs (NSAIDs) play an important role, particularly where there is a significant inflammatory component ... [more ▼]

Analgesics continue to be the mainstay of therapy in osteoarthritis. Non-steroidal anti-inflammatory drugs (NSAIDs) play an important role, particularly where there is a significant inflammatory component to the osteoarthritis. Piroxicam-beta-cyclodextrin (PBC) is a new formulation in which piroxicam has been complexed with beta-cyclodextrin, a cyclic oligosaccharide. This results in an increase in the rate of absorption of the active compound and, consequently, in an earlier onset of analgesic action. PBC, like piroxicam, is administered once daily. PBC has been used in the treatment of osteoarthritis. In comparison with piroxicam, PBC showed a more rapid analgesic-anti-inflammatory action after the first administration in patients with active osteoarthritis. Subsequent evaluations at the second, fifth and last day of treatment demonstrated a comparable efficacy of the two drugs. The efficacy and tolerability of PBC was compared with other NSAIDs given intramuscularly, such as diclofenac and ketoprofen. The three compounds provided marked pain relief within thirty minutes and this increased progressively until the third to fourth hour. The efficacy of oral PBC was comparable to that of intramuscular diclofenac or ketoprofen. In comparison with metamisole PBC achieved a more rapid and sustained reduction in pain intensity during the first twelve hours of treatment. This rapid and marked reduction in pain intensity with PBC was also observed in patients with low-back pain when compared with etodolac. In view of its efficacy, tolerability and rapid onset of action, piroxicam-beta-cyclodextrin appears to be an useful analgesic and a prominent progress in the treatment of acute rheumatic pain. [less ▲]

Detailed reference viewed: 22 (1 ULg)
See detailPiaget et la pédagogie : une confrontation difficile, mais prometteuse
Crahay, Marcel ULg

in Apprendizagem/Disenvolvimento (1993), III(13/14),

Detailed reference viewed: 17 (2 ULg)
Full Text
Peer Reviewed
See detaila new way to solve galloping on bundled lines. A concept, a prototype bytwo years field experience.
Lilien, Jean-Louis ULg; pirotte, pol; smart, Tom et al

in Bulletin Scientifique de l'Association des Ingénieurs Electriciens sortis de l'Institut Electrotechnique Montefiore (1993), 2

that paper introduce a new concept to control galloping on bundle conductors using a dynamic device coupling detuning of frequencies together with some addition in torsional damping.The concept is ... [more ▼]

that paper introduce a new concept to control galloping on bundle conductors using a dynamic device coupling detuning of frequencies together with some addition in torsional damping.The concept is explained, a prototype is tested on actual line and results are presented. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailA new topical treatment for resistant herpes simplex infections.
Snoeck, R.; Andrei, G.; De Clercq, E. et al

in New England Journal of Medicine [=NEJM] (1993), 329(13), 968-9

Detailed reference viewed: 15 (0 ULg)
Peer Reviewed
See detailTabagisme et poids corporel.
PAQUOT, Nicolas ULg; SCHEEN, André ULg; Lefebvre, P.

in Médecine et Hygiène (1993), 51

Detailed reference viewed: 12 (3 ULg)
Peer Reviewed
See detailStimulation of the 92-Kd Type Iv Collagenase Promoter and Enzyme Expression in Human Melanoma Cells
Lauricella-Lefebvre, M. A.; Castronovo, Vincenzo ULg; Sato, H. et al

in Invasion & Metastasis (1993), 13(6), 289-300

The 92-kD type IV collagenase is a member of the metalloproteinase family which degrades type IV collagen, a major component of basement membrane and is involved in tumor invasion and metastasis. The ... [more ▼]

The 92-kD type IV collagenase is a member of the metalloproteinase family which degrades type IV collagen, a major component of basement membrane and is involved in tumor invasion and metastasis. The promoter and adjacent regulatory sequences of the 92-kD type IV collagenase have been identified previously and three cis-acting elements homologous to the binding sites for AP-1, NF-KB and SP-1 proteins contributed to induction of the promoter activity by 12-O-tetradecanoylphorbol 13-acetate (TPA) and tumor necrosis factor (TNF-alpha) in HT1080 cells. To date, no direct correlation between promoter activity and expression of the 92-kD type IV collagenase has been reported in normal or cancer cells. In this study, the effects of the transcriptional stimulation of the 92-kD type IV collagenase gene on the expression of the enzyme in human A2058 melanoma cells was analyzed by zymography experiments. Quantitative immunoblots using a monoclonal antibody that recognized specifically and exclusively the 92-kD type IV collagenase, confirmed that the 92-kD gelatinase was 92-kD type IV collagenase. Stimulation of the promoter activity resulted in increased gelatinase activity in the culture medium of A2058 cells. A direct correlation between TPA- and TNF-alpha-mediated promoter stimulation of the 92-kD type IV collagenase gene and its expression was also demonstrated in the human fibrosarcoma HT1080 cells. Interleukin-1 alpha failed to induce 92-kD gene promoter activity and type IV collagenase expression in melanoma and fibrosarcoma cell lines. Our data demonstrated that TPA- and TNF-alpha-induced 92-kD type IV collagenase promoter stimulation leads to a proportional increase of enzyme expression and secretion and thus could contribute to the activation of the invasive phenotype. [less ▲]

Detailed reference viewed: 23 (7 ULg)
Full Text
Peer Reviewed
See detailTi-ii transition probabilities and radiative lifetimes in TI and the solar titanium abundance
Bizzarri, A.; Huber, M. C. E.; Noels-Grötsch, Arlette ULg et al

in Astronomy and Astrophysics (1993), 273

Transition probabilities of 100 Ti-II emission lines, originating from 7 different atomic levels, have been determined by combining branching fractions with radiative lifetimes. The branching fractions ... [more ▼]

Transition probabilities of 100 Ti-II emission lines, originating from 7 different atomic levels, have been determined by combining branching fractions with radiative lifetimes. The branching fractions were measured using Fourier transform spectroscopy on a hollow cathode. The radiative lifetimes of these 7 - and 35 additional - levels were measured using time resolved laser-induced fluorescence on a slow Ti ion beam. The transition probabilities of 21 very weak lines have been used to derive a solar titanium abundance of α[SUB]Ti[/SUB] = log(N[SUB]Ti[/SUB]/N[SUB]H[/SUB]) + 12=5.04±0.04 dex, which is insensitive to the solar model. This value is in disagreement with the meteoritic titanium abundance (4.93±0.02). [less ▲]

Detailed reference viewed: 6 (0 ULg)
See detailExperts et novices face à un circuit simulé sur ordinateur
Lafontaine, Dominique ULg; Blondin, Christiane ULg; Closset, Jean-Louis ULg

in Education et Recherche (1993), 1

Detailed reference viewed: 19 (13 ULg)
Full Text
Peer Reviewed
See detailClonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(6), 427-431

Detailed reference viewed: 19 (5 ULg)
Peer Reviewed
See detailTemporal reasoning and reasoning theories : a case study in anaesthesiology
Sougné, Jacques ULg; Nyssen, Anne-Sophie ULg; De Keyser, Véronique ULg

in Psychologica Belgica (1993), 33(2), 311-328

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that ... [more ▼]

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that appear in working situations and even, understanding and preventing some human errors. In order ot illustrate theoritical concepts, we will provide examples of a situation in which the dynamic character of the evolution, the critical character of the planning and of the synchronization force temporal reasoning. This situation will be that of anaesthesia. [less ▲]

Detailed reference viewed: 22 (2 ULg)
See detailLa morphologie synchronique du nom latin. Une bibliographie de base (1962-1991)
Pietquin, Paul ULg; Isebaert, Lambert

in Orbis : Bulletin international de documentation linguistique (1993), 36

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailAspects cliniques de la néoplasie endocrinienne multiple de type 1 (NEM-1)
Ghuysen, Alexandre ULg; Beckers, Albert ULg; Stevenaert, Achille ULg

in Médecine & Chirurgie Digestives (1993), 22(8), 468-470

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailAtomic data and the spectrum of the solar photosphere.
Grevesse, Nicolas ULg; Noels-Grötsch, Arlette ULg

in Physica Scripta (1993), 47

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the ... [more ▼]

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the atomic data, especially the transition probabilities for faint lines. The authors justify this request for many more very accurate gf-values through a few particularly striking points. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailLasfe (CIL IV 2319 l) et quelques autres exemples d’usage abusif de l’épigraphie en phonétique historique
Purnelle, Gérald ULg

in Etudes Classiques (Namur, Belgium) (1993), 61

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailThe peripheral analgesic effects of opiods
Joris, Jean ULg; Hargreaves, Kenneth M

in APS Journal (1993), 2(1), 51-59

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailA comparative study of class-D beta-lactamases.
Ledent, Philippe; Raquet, X; Joris, Bernard ULg et al

in Biochemical Journal (1993), 292 ( Pt 2)

Three class-D beta-lactamases (OXA2, OXA1 and PSE2) were produced and purified to protein homogeneity. 6 beta-Iodopenicillanate inactivated the OXA2 enzyme without detectable turnover. Labelling of the ... [more ▼]

Three class-D beta-lactamases (OXA2, OXA1 and PSE2) were produced and purified to protein homogeneity. 6 beta-Iodopenicillanate inactivated the OXA2 enzyme without detectable turnover. Labelling of the same beta-lactamase with 6 beta-iodo[3H]penicillanate allowed the identification of Ser-70 as the active-site serine residue. In agreement with previous reports, the apparent M(r) of the OXA2 enzyme as determined by molecular-sieve filtration, was significantly higher than that deduced from the gene sequence, but this was not due to an equilibrium between a monomer and a dimer. The heterogeneity of the OXA2 beta-lactamase on ion-exchange chromatography contrasted with the similarity of the catalytic properties of the various forms. A first overview of the enzymic properties of the three 'oxacillinases' is presented. With the OXA2 enzyme, 'burst' kinetics, implying branched pathways, seemed to prevail with many substrates. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailMechanism of action of DD-peptidases: role of asparagine-161 in the Streptomyces R61 DD-peptidase.
Wilkin, J M; Jamin, M; Joris, Bernard ULg et al

in Biochemical Journal (1993), 293 ( Pt 1)

The role of residue Asn-161 in the interaction between the Streptomyces R61 DD-peptidase and various substrates or beta-lactam inactivators was probed by site-directed mutagenesis. The residue was ... [more ▼]

The role of residue Asn-161 in the interaction between the Streptomyces R61 DD-peptidase and various substrates or beta-lactam inactivators was probed by site-directed mutagenesis. The residue was successively replaced by serine and alanine. In the first case, acylation rates were mainly affected with the peptide and ester substrates but not with the thiol-ester substrates and beta-lactams. However, the deacylation rates were decreased 10-30-fold with the substrates yielding benzoylglycyl and benzoylalanyl adducts. The Asn161Ala mutant was more generally affected, although the acylation rates with cefuroxime and cefotaxime remained similar to those observed with the wild-type enzyme. Surprisingly, the deacylation rates of the benzoylglycyl and benzoylalanyl adducts were very close to those observed with the wild-type enzyme. The results also indicate that the interaction with the peptide substrate and the transpeptidation reaction were more sensitive to the mutations than the other reactions studied. The results are discussed and compared with those obtained with the Asn-132 mutants of a class A beta-lactamase. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailA sulfonylthiourea (BM 20) related to torasemide: a new loop diuretic with relative potassium-sparing properties
Masereel, B.; Schynts, M.; Krzesinski, Jean-Marie ULg et al

in Journal of Pharmacy & Pharmacology (1993), 45

A series of sulphonylthioureas related to torasemide, a high ceiling loop diuretic, were synthesized and found to inhibit the Na+ 2Cl- K+ co-transporter of the thick ascending limb of the loop of Henlé ... [more ▼]

A series of sulphonylthioureas related to torasemide, a high ceiling loop diuretic, were synthesized and found to inhibit the Na+ 2Cl- K+ co-transporter of the thick ascending limb of the loop of Henlé. Their diuretic properties were studied (30 mg kg-1) after oral administration to rats. Lipophilic derivatives very active in-vitro, were found inactive orally and intraperitoneally in rats. The four most active compounds were examined for their dose-dependent diuresis. Three of them showed a potency, water and electrolyte excretion similar to torasemide. The fourth molecule, a sulphonylthiourea (BM 20), exhibited relative potassium-sparing properties and a minimal diuretic dose of 0.001 mg kg-1, 200 times lower than torasemide. [less ▲]

Detailed reference viewed: 17 (4 ULg)
Full Text
Peer Reviewed
See detailBiostratigraphy and sequence stratigraphy at the Devonian-Carboniferous transition in southern China (Hunan Province); comparison with southern Belgium
Hance, Luc ULg; Muchez, P; Coen, M et al

in Annales de la Société Géologique de Belgique (1993), 116(2), 359-378

Detailed reference viewed: 9 (1 ULg)
Peer Reviewed
See detailPharmacological treatment of the obese diabetic patient.
Scheen, André ULg; Lefebvre, Pierre ULg

in Diabète & Métabolisme (1993), 19(6), 547-59

Obesity is a well-known risk factor for non-insulin-dependent (or Type 2) diabetes mellitus. Consequently, reduction of weight excess comes to the front line in the prevention and management of NIDDM. It ... [more ▼]

Obesity is a well-known risk factor for non-insulin-dependent (or Type 2) diabetes mellitus. Consequently, reduction of weight excess comes to the front line in the prevention and management of NIDDM. It is only when diet and physical exercise fail that drug treatment should be considered. Pharmacological treatment of obesity should favour drugs which not only promote weight loss, by reducing caloric intake and/or increasing thermogenesis and energy expenditure, but also, and especially, improve insulin sensitivity. Serotoninergic anorectic compounds (dexfenfluramine, fluoxetine) appear to possess, to some extent, all these properties. Metformin significantly reduces insulin resistance and improves glycaemic control without inducing weight gain, and even favouring some weight loss. This biguanide is now considered as the first line drug for the obese diabetic patient. Alpha-glucosidase inhibitors may help to reduce post-prandial glucose excursions but do not promote weight loss per se. Sulfonylureas can be prescribed to an obese patient when hyperglycaemia persists despite diet and the above-mentioned oral agents, but their use should be associated with reinforcement of dietary advices in order to prevent further weight increase; it is also the case for insulin therapy. Finally, drugs specifically stimulating thermogenesis and energy expenditure, new agents sensitizing tissues to the action of insulin and various compounds interfering with lipid metabolism are currently under extensive investigation with promising preliminary results in the obese diabetic patient. In conclusion, obesity remains a major problem in the management of Type 2 diabetes mellitus and this justifies the search for new, safe and effective, pharmacological approaches. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Peer Reviewed
See detailThe use of the soil map of Belgium in the assessment of landslide risk
Closson, Damien; Engels, Patrick ULg; Demaret, Xavier

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(3), 165172

Detailed reference viewed: 7 (3 ULg)
Full Text
Peer Reviewed
See detailPurification Of Antifungal Lipopeptides By Reversed-Phase High-Performance Liquid-Chromatography
Razafindralambo, Hary ULg; Paquot, Michel ULg; Hbid, Choukri et al

in Journal of Chromatography. A (1993), 639(1), 81-85

A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C18 gel ... [more ▼]

A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C18 gel followed by reversed-phase chromatography using a biocompatible PepRPC HR 515 column with a pharmacia fast protein liquid chromatographic system. This is a very effective method for isolating and fractionating iturin A and surfactin, two lipopeptides of different nature, co-produced by Bacillus subtilis strain S499. The presence of homologous lipopeptides was easily detected. [less ▲]

Detailed reference viewed: 109 (37 ULg)
Peer Reviewed
See detailEchographic Characterization of Carotid Plaques
VAN DAMME, Hendrik ULg; TROTTEUR, Geneviève ULg; Vivario, M. et al

in Acta Chirurgica Belgica (1993), 93(5), 233-8

The histology of the endarterectomy specimen was compared with the preoperative echopattern of 154 plaques. Thirty-four ultrasound images were of poor quality. The 120 adequate echographies revealed 22 ... [more ▼]

The histology of the endarterectomy specimen was compared with the preoperative echopattern of 154 plaques. Thirty-four ultrasound images were of poor quality. The 120 adequate echographies revealed 22 dense hyperechogenic homogeneous plaques, 28 soft hypoechogenic homogeneous plaques, 53 inhomogeneous plaques, and 13 mixed plaques. According to the surface, lesions were subdivided as smooth (n = 63) or irregular (n = 57). Echography recognized fibrous plaques (dense homogeneous hyperechogeneous lesions), with a specificity of 87% and a sensitivity of 56%. Recent intraplaque haemorrhage is echographically apparent as a hypoechogenic area in 88% of cases, what corresponds to a specificity of 79% and a sensitivity of 75%. Echography proved to be more accurate than angiography in determining the presence or absence of ulceration. The ability to characterize the pathology of carotid atheroma by duplex scan make the clinicopathologic correlation of carotid plaques of practical importance. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Peer Reviewed
See detailAccelerated erythroid repopulation with no stem-cell competition effect in children treated with recombinant human erythropoietin after allogeneic bone marrow transplantation.
Locatelli, F.; Zecca, M.; Beguin, Yves ULg et al

in British Journal of Haematology (1993), 84(4), 752-4

We carried out a pilot study on the use of recombinant human erythropoietin (rHuEPO) to accelerate erythropoietic engraftment in paediatric patients undergoing allogeneic BMT. rHuEPO was administered ... [more ▼]

We carried out a pilot study on the use of recombinant human erythropoietin (rHuEPO) to accelerate erythropoietic engraftment in paediatric patients undergoing allogeneic BMT. rHuEPO was administered intravenously at a dose of 75 U/kg per day for 30 d after transplant. Erythroid repopulation, evaluated sequentially through the serum transferrin receptor, was faster in 15 patients receiving rHuEPO than in 16 historical controls (P = 0.0003). This faster erythroid engraftment resulted in a reduction in the total number of red blood cell units required to reach transfusion independence (2.7 +/- 1.2 v 4.2 +/- 2.3, P = 0.027). No significant difference in leucocyte or platelet regeneration was observed. These findings indicate that rHuEPO administration can accelerate erythroid recovery after allogeneic BMT and reduce red cell transfusion requirements with no stem-cell competition effect. [less ▲]

Detailed reference viewed: 15 (2 ULg)
Peer Reviewed
See detailHyperlipidemies et medicaments hypolipidemiants.
Scheen, André ULg; Paquot, Nicolas ULg

in Journal de Pharmacie de Belgique (1993), 48(2), 92-101

Hyperlipidaemia, as a primary atherogenic risk factor, represents a major problem of public health. This review first reminds the main steps of lipoprotein metabolism, the classification of the most ... [more ▼]

Hyperlipidaemia, as a primary atherogenic risk factor, represents a major problem of public health. This review first reminds the main steps of lipoprotein metabolism, the classification of the most frequent hyperlipidaemias, the objectives of the treatment and the required initial evaluation allowing to decide how to manage the patient. Thereafter, it describes all the available treatments, more particularly the characteristics of the various lipid lowering drugs. Finally, it proposes a step by step strategy for the treatment of the main hyperlipidaemias and summarizes the managements of some particular cases. [less ▲]

Detailed reference viewed: 49 (0 ULg)
Peer Reviewed
See detailL'histoire de Méléagre : plaidoyer pour une analyse de l'ensemble du mythe
Renaud, Jean-Michel ULg

in Kernos : Revue Internationale et Pluridisciplinaire de Religion Grecque (1993), 6

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailThe Audiospinal Reaction in Parkinsonian Patients Reflects Functional Changes in Reticular Nuclei
Delwaide, Paul ULg; PEPIN, Jean-Louis ULg; Maertens De Noordhout, Alain ULg

in Annals of Neurology (1993), 33(1), 63-9

Audiospinal facilitation using the soleus H-reflex as a test was compared in 16 control subjects and 23 parkinsonian patients. In the patients, facilitation was significantly reduced during the 75 to 150 ... [more ▼]

Audiospinal facilitation using the soleus H-reflex as a test was compared in 16 control subjects and 23 parkinsonian patients. In the patients, facilitation was significantly reduced during the 75 to 150 msec after the conditioning stimulation. This reduction was seen bilaterally even in patients with a hemisyndrome. It was corrected by L-dopa but not by anticholinergic agents. Facilitation at the 75-msec delay showed an inverse linear correlation with the bradykinesia intensity (r = -0.7, p < 0.01). The results argue in favor of a reduced excitability of the nucleus reticularis pontis caudalis from which a reticulospinal tract emanates as effector of the audiospinal facilitation. This would represent another example of dysfunction of reticular nuclei in Parkinson's disease. [less ▲]

Detailed reference viewed: 14 (9 ULg)
Full Text
Peer Reviewed
See detailMonoamine Oxidase Inhibitors and Anesthesia: An Updated Literature Review
Blom-Peters, L.; Lamy, Maurice ULg

in Acta Anaesthesiologica Belgica (1993), 44(2), 57-60

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these ... [more ▼]

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these anesthetics. These drugs allow selective and rapidly reversible inhibition of type A-MAO. The pharmacologic effects of this new class of drug as well as their potential interactions with agents used in anesthesia are reviewed. [less ▲]

Detailed reference viewed: 61 (1 ULg)
See detailDe Dakconstructie van de Karmelietenkerk, eerste dendrochronologische analyse
Hoffsummer, Patrick ULg; Houbrechts, David

in Stadsarcheologie (1993), 17(2), 29-34

Detailed reference viewed: 7 (0 ULg)
See detailLa decharge egale un bioreacteur a gerer.
Dumont, M.; Taillieu, X.; Destain, Jacqueline ULg et al

in Tribune de l'Eau (La) (1993), 46(564), 3-10

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailn < 2 Allowed Transitions in Neutral Sulfur within in the Visible and Infrared Spectral Ranges
Biémont, Emile ULg; Quinet, Pascal ULg; Zeippen, C. J.

in Astronomy and Astrophysics. Supplement Series (1993), 102

Detailed reference viewed: 3 (0 ULg)
Peer Reviewed
See detailUltrastructural distribution of DNA within the nucleolus of various animal cell lines or tissues revealed by terminal deoxynucleotidyl transferase.
Thiry, Marc ULg; Ploton, D.; Menager, M. et al

in Cell & Tissue Research (1993), 271(1), 33-45

We have used the highly sensitive in situ terminal deoxynucleotidyl transferase method, applied to ultrathin sections, to investigate the location of DNA within nucleoli of various animal cells. In all ... [more ▼]

We have used the highly sensitive in situ terminal deoxynucleotidyl transferase method, applied to ultrathin sections, to investigate the location of DNA within nucleoli of various animal cells. In all the nucleoli studied, intense labelling is revealed over the peri- and intranucleolar condensed chromatin. Gold particles are also consistently found over the fibrillar centres, especially at their periphery, namely in the border area between the fibrillar centres and the dense fibrillar component, whereas the dense fibrillar component itself seems to be free of label in nucleoli in which these two compartments can be distinguished. We conclude that, in transcriptionally active nucleoli of this type, DNA is a characteristic constituent of the fibrillar centres, distinguishing them functionally from the dense fibrillar component. Some nucleoli exhibit neither fibrillar centres nor a dense fibrillar component, but have a single, albeit heterogeneous accumulation of fibrillar material; gold particles are consistently seen over some parts of this fibrillar compartment. This suggests that certain parts of the fibrillar material are functionally similar to the fibrillar centres of those nucleoli that possess them. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
See detailRéussite de quatre nichées successives chez le martin pêcheur d'Europe (Alcedo atthis) au cours de la même saison
Libois, Roland ULg

in Aves (1993), 30(1), 31-36

From 1985 to 1992, 229 breeding pairs of kingfisher were studied to determine the reproduction success and the way population recovered after the severe winter 1984-85. From these investigations, we were ... [more ▼]

From 1985 to 1992, 229 breeding pairs of kingfisher were studied to determine the reproduction success and the way population recovered after the severe winter 1984-85. From these investigations, we were able to observe four successive nesting attempts by five different pairs. Four broods were raised by one of the pairs. As the adult were ringed birds and were regularky checked throughout the breeding season, ther can be no doubt, now, as to the possibility of a fourth brood for this species, at least in peculiar circumstances. [less ▲]

Detailed reference viewed: 26 (2 ULg)
Full Text
Peer Reviewed
See detailComparing measures of poverty and relative deprivation: an example for Belgium
Delhausse, Bernard; Luttgens, Axel; Perelman, Sergio ULg

in Journal of Population Economics (1993), 6

This paper presents three different poverty standards. A first approach takes the disposable income as an indicator of poverty. A second approach uses the Leyden approach. Finally an aggregate index of ... [more ▼]

This paper presents three different poverty standards. A first approach takes the disposable income as an indicator of poverty. A second approach uses the Leyden approach. Finally an aggregate index of deprivation, based on the observation of consumption events, is constructed through a particular econometric procedure proposed by Desai and Shah (1988). These alternative measures are then compared on a sample composed of 6380 Belgian households. Such an analysis can be expected to provide some further insight into the problem of measuring poverty, which has been the subject of a recent controversial debate. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailRestriction Maps of the DNA of Cervid Herpesvirus 1 and Cervid Herpesvirus 2, Two Viruses Related to Bovine Herpesvirus 1
Vanderplasschen, Alain ULg; Bublot, M.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 128(3-4), 379-88

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as ... [more ▼]

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailEnhancement of bovine leukemia virus-induced syncytia formation by di- and tripeptides.
Voneche, V.; Callebaut, I.; Lambrecht, B. et al

in Virology (1993), 192(1),

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by ... [more ▼]

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by bovine leukemia virus (BLV). Peptides containing a hydrophobic contribution and/or a positive net charge strongly enhanced syncytia formation induced by BLV on CC81 indicator cells. The size of the multinucleated cells was strongly increased (up to 10-fold) in the presence of the enhancer peptides whereas no effect was observed on the indicator cells in the absence of BLV. The peptides thus amplified the fusion process initiated by BLV envelope glycoproteins. The effect was dose-dependent at concentrations ranging from 20 to 640 microM and did not result from an increased expression of BLV proteins. The peptides did not compete with anti-gp51 monoclonal antibodies for the recognition of eight well-defined epitopes of gp51. We consequently hypothesize that the enhancer peptides interact with the membrane of BLV-producing cells and/or indicator cells and propose a model based on molecular modeling. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailLa place de la musique en hôpital de jour
Triffaux, Jean-Marc ULg; Bertrand, Jean ULg

in Psychologie Médicale (1993)

Detailed reference viewed: 41 (4 ULg)
Peer Reviewed
See detailImmunodetection of RNA on ultra-thin sections incubated with polyadenylate nucleotidyl transferase.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1993), 41(5), 657-65

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and ... [more ▼]

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and biotinylated ATP. The labeled nucleotides bound to RNA at the surface of the ultra-thin sections were than visualized by an indirect immunogold labeling technique. The resulting labeling pattern was dependent on the presence of divalent cations in the PnT medium. The method revealed with great precision the specific RNA-containing structures within Ehrlich tumor cells. The method is applicable to Epon sections. However, the labeling intensity varies according to the fixation used. Best results were obtained on acetylated cell sections. The method can be combined with EDTA regressive staining. The in situ PnT method provides a very useful tool for pinpointing the precise location of RNA within biological material at the ultrastructural level. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailSynthetic model peptides for apolipoproteins. II. Characterization of the discoidal complexes generated between phospholipids and synthetic model peptides for apolipoproteins.
Corijn, J.; Deleys, R.; Labeur, C. et al

in Biochimica et Biophysica Acta (1993), 1170(1), 8-16

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied ... [more ▼]

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied. The sequences of the peptides were derived from that of the 18A peptide and designed to either enhance or decrease ionic interactions between pairs of peptides, as described in the accompanying paper. Complexes were prepared with dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), or with DPPC and cholesterol, and isolated on a Superose 6HR column. Association kinetics for the DMPC-peptides complexes were followed by measuring the turbidity as a function of the temperature. The diameters of the DPPC-peptide complexes, measured by gradient gel electrophoresis (GGE), were about 120 A. Fluorescence polarization measurements after labeling with diphenyl hexatriene (DPH) yielded transition temperatures of, respectively, 40.6, 41.5 and 41.8 degrees C for the DPPC/18AM1-, DPPC/18AM4- and DPPC/18A-peptide complexes. These values were confirmed by differential scanning calorimetry. Circular dichroism and infrared spectroscopy revealed that the peptides adopt an alpha-helical structure in solution and this percentage increased from 30-40% in the free peptides up to 50-60% in the complexes. Attenuated total reflection (ATR) infrared measurements of the complexes indicated that the peptides are oriented parallel to the acyl chains of the phospholipid bilayer. Denaturation of the peptides and of the peptide-lipid complexes was monitored by Trp fluorescence under addition of increasing amounts of GdmCl. The mid-points of the denaturation curves lie at, respectively, 0.05, 0.25 and 0.35 M GdmCl for the 18AM4, 18A and 18AM1 peptide and are shifted towards higher GdmCl concentrations after peptide-lipid binding. GdmCl denaturation decreased the alpha-helical content of the peptides and of the complexes, as monitored by circular dichroism measurement. The helix to random coil structure transition occurred at, respectively, 2.1, 2.2, and 2.0 M GdmCl for 18A, 18AM1 and 18AM4, compared to 5.1, 5.0, and 5.3 M in the corresponding complexes. These data suggest altogether that the structural properties, the mode of lipid-protein association and the stability of the phospholipid-peptide complexes are similar to those of native plasma apolipoproteins. The 18A and 18AM4 peptides which contain charged residues along the edge of the helix, leading to salt bridge formation between peptides were shown to mimic the amphipathic helices of the plasma apolipoproteins. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Peer Reviewed
See detailSequencing of a 52 kb yeast DNA located on chromosome XI of Saccharomyces cerevisiae and preliminary study of a new essential ORF.
Bolle, Paul André; Vandenbol, Micheline ULg; Portetelle, Daniel ULg et al

in Archives Internationales de Physiologie et de Biochimie (1993)

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailApplying the Lagamine model to compute land subsidence in Shanghai
Dassargues, Alain ULg; Schroeder, Christian ULg; Li, Xiang Lin

in Bulletin of Engineering Geology & the Environment (1993), 47

Detailed reference viewed: 54 (11 ULg)
Peer Reviewed
See detailSurgical Technique for Cardiomyoplasty and Iterative Transthoracic Biopsies in an Experimental Goat Model
Radermecker, M. A.; Fourny, J.; Bonnet, Pierre ULg et al

in European Surgical Research = Europäische Chirurgische Forschung = Recherches Chirurgicales Européennes (1993), 25(2, Mar-Apr), 110-22

Cardiomyoplasty is a new surgical procedure using transformed skeletal muscle to reinforce or substitute for the myocardium. For the purpose of studying surgical technique and investigating basic ... [more ▼]

Cardiomyoplasty is a new surgical procedure using transformed skeletal muscle to reinforce or substitute for the myocardium. For the purpose of studying surgical technique and investigating basic questions, we have developed a model of reinforcement cardiomyoplasty. Herein, we describe anesthetic management, surgical technique, and the technique for iterative transthoracic biopsies. This model has proven to be valuable in several long-term studies involving more than 30 goats. Preliminary data to ascertain the efficacy of the proposed technique are provided. [less ▲]

Detailed reference viewed: 11 (2 ULg)