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See detailNo significant effect of an "atraumatic" needle on incidence of post-lumbar puncture headache or traumatic tap.
Lenaerts, M.; Pepin, J. L.; Tombu, S. et al

in Cephalalgia : An International Journal of Headache (1993), 13(4), 296-7

We compared the incidence of post-lumbar puncture headache and of red blood cells in the first CSF sample (traumatic tap) in 35 inpatients punctured with a standard 20 G Yale needle and in 26 patients ... [more ▼]

We compared the incidence of post-lumbar puncture headache and of red blood cells in the first CSF sample (traumatic tap) in 35 inpatients punctured with a standard 20 G Yale needle and in 26 patients punctured with Sprotte's "atraumatic" needle. No significant difference was found between the two groups in incidence of headache or in frequency of traumatic taps. [less ▲]

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See detailScaled effective chiral lagrangians for the propagation of scalar and vector mesons in dense matter
Jaminon, Martine ULg; Ripka, Georges

in Nuclear Physics A (1993), 564

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See detailSaving behavior in 17 OECD countries
Kessler, Denis; Pestieau, Pierre ULg; Perelman, Sergio ULg

in Review of Income and Wealth (1993), (39), 37-50

This article compares savings behavior in a sample of 17 OECD countries over 24 years. On the basis of an analysis of variance and of a life-cycle-hypothesis-based equation, we test the homogeneity of ... [more ▼]

This article compares savings behavior in a sample of 17 OECD countries over 24 years. On the basis of an analysis of variance and of a life-cycle-hypothesis-based equation, we test the homogeneity of households' savings behavior. It appears that one cannot really speak of a homogeneous saving behavior across countries. This is a relevant finding in times of increasing economic and financial integration. [less ▲]

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See detailIdentification of eae genes in rabbit attaching and effacing enteropathogenic Escherichia coli (EPEC)
Pohl, P.; Peeters, J. E.; Jacquemin, E. R. et al

in Infection and Immunity (1993), 61

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See detailComputed subsidence of the central area of Shanghai
Dassargues, Alain ULg; Radu, Jean-Pol ULg; Charlier, Robert ULg et al

in Bulletin of Engineering Geology & the Environment (1993), 47

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See detailEfficient lipofection of human trophoblast cells in primary cultures
Jacquemin, P.; Alsat, E.; Oury, Cécile ULg et al

in Biochemical and Biophysical Research Communications (1993), 196(1), 376-81

Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this hormone ... [more ▼]

Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this hormone, we became interested in transfecting primary cultures of these trophoblast cells. In this study, we show that it is possible to transfect, by the lipofection method, these giant cells in an efficient and reproducible manner. We show the presence of an enhancer region downstream from the hCS-B gene, functionally active in these cells; furthermore, we demonstrate the placenta-specific characteristic of this enhancer, previously identified in a human choriocarcinoma cell line. [less ▲]

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See detailNUCLEOPHILIC ENANTIOSELECTIVE SYNTHESIS OF 6-[F-18]FLUORO-L-DOPA VIA 2 CHIRAL AUXILIARIES
Lemaire, Christian ULg; Plenevaux, Alain ULg; Cantineau, Robert et al

in Applied Radiation & Isotopes (1993), 44(4), 737-744

Asymmetric nucleophilic synthesis of 6-[F-18]fluoro-L-dopa was investigated in order to reach an enantiomeric excess of close to 100% of the L form of this amino acid. The radiochemical synthesis required ... [more ▼]

Asymmetric nucleophilic synthesis of 6-[F-18]fluoro-L-dopa was investigated in order to reach an enantiomeric excess of close to 100% of the L form of this amino acid. The radiochemical synthesis required [F-18]fluoride as fluorinating agent and regioselective nucleophilic substitution of commercially available 6-nitroveratraldehyde. The [F-18]fluorobenzaldehyde thus obtained was easily converted to the corresponding 2-[F-18]fluoro-4,5-dimethoxybenzyl bromide. This alkylating agent was added to the lithium enolates of 1-(S)-(-)camphor imine of t-butyl glycinate (1) and (S)-(-)- 1 -Boc-2-t-butyl-3-methyl-4-imidazolidinone [(S)- Boc-BMI] (2) in order to compare the enantiomeric excess of the L form obtained in each case with these two chiral inductors. The L-isomer of fluorodopa was isolated after H1 hydrolysis and HPLC purification in 5-10% radiochemical yield (decay corrected). The overall synthesis time was of 110 min. Through this synthetic pathway, the L-isomer of fluorodopa was obtained in 83% e.e with 1 and 96% e.e with 2 respectively, as determined by chiral HPLC. A practical three step preparative scale synthesis of 6-[F-19]fluoro-D,L-dopa is also presented. [less ▲]

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See detailPhysiologie de la cellule enterochromaffin-like (ECL) dans la régulation de la sécrétion gastrique acide
Delwaide, Jean ULg; Belaiche, Jacques ULg; Latour, Pascale ULg

in Médecine & Chirurgie Digestives (1993), 22

Cette mise au point rappellera d'abord les grands mécanismes de la sécrétion gastrique acide en insistant sur le rôle pivot de l'histamine. Une question qui demeurait encore non résolue était de savoir ... [more ▼]

Cette mise au point rappellera d'abord les grands mécanismes de la sécrétion gastrique acide en insistant sur le rôle pivot de l'histamine. Une question qui demeurait encore non résolue était de savoir quelle était la cellule fundique qui produisait de l'histamine dans les conditions physiologiques. Deux cellules seront envisagées, les mastocytes et les cellules ECL qui sont des cellules endocrines exclusivement localisées dans le fundus. Quelques arguments seront développés, dont ceux provenant de notre expérience, permettant de penser que la cellule ECL contient de l'histamine. L'article se terminera par un essai d'intégration de la cellule ECL dans le schéma de la sécrétion gastrique acide en montrant son rôle essentiel potentiel. [less ▲]

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See detailBiometrical evaluation of the stratum corneum texture in patients under maintenane hemodialysis
Deleixhe-Mauhin, F.; Pierard, Claudine ULg; Krzesinski, Jean-Marie ULg et al

in Nephron (1993), 64

Xerosis and hydration of the stratum corneum were evaluated in 60 hemodialyzed patients. Xerosis and a low capacitance of the stratum corneum were evidenced in more than 80% of the patients. Pruritus was ... [more ▼]

Xerosis and hydration of the stratum corneum were evaluated in 60 hemodialyzed patients. Xerosis and a low capacitance of the stratum corneum were evidenced in more than 80% of the patients. Pruritus was present in two third of the subjects. We failed to disclose any significant relationship between severity of these three parameters. [less ▲]

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See detailRapport préliminaire sur la 2e campagne de fouille à Tell Amarna (Syrie)
Tunca, Önhan ULg

in Akkadica (1993), 83

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See detailThe Appearance of C2H3+ Ions from C2H3F by non-resonant Photoionization. A Translational Energy Surprisal Analysis.
Momigny, J.; Locht, Robert ULg

in Chemical Physics Letters (1993), 211(2,3), 161-165

It is shown that for a photon energy of 16.85 eV the translational energy surprisal of the C2H3++F dissociation products from C2H3F+ has a bimodal behaviour. The possibility of considering the bimodal ... [more ▼]

It is shown that for a photon energy of 16.85 eV the translational energy surprisal of the C2H3++F dissociation products from C2H3F+ has a bimodal behaviour. The possibility of considering the bimodal surprisal as due to the superposition of two translational energy distributions is consistent with the theoretical assumption of the concurrence of two channels in this dissociation process. The characteristics of these distributions, i.e. the energy width and the relative abundance, are in agreement with the results of an ab initio quantum-mechanical calculation of the dynamics of this dissociation. They are consistent with the simultaneous occurrence of a statistical dissociation of C2H3F+(X2A') into C2H3+(X1A') and an isolated state dissociation of C2H3F+(C2A") into C2H3+(a3A"). Negative lambda values obtained for the surprisal of both these distributions indicate an inversion of the translational level population of the dissociation products. [less ▲]

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See detailTemporal reasoning and reasoning theories : a case study in anaesthesiology
Sougné, Jacques ULg; Nyssen, Anne-Sophie ULg; De Keyser, Véronique ULg

in Psychologica Belgica (1993), 33(2), 311-328

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that ... [more ▼]

The purpose of this article is to clarify the theoritical base necessary for the design of a computer-based simulation of temporal reasoning. Simulation allows a better understanding of phenomena that appear in working situations and even, understanding and preventing some human errors. In order ot illustrate theoritical concepts, we will provide examples of a situation in which the dynamic character of the evolution, the critical character of the planning and of the synchronization force temporal reasoning. This situation will be that of anaesthesia. [less ▲]

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See detailHEXAGONAL MARANGONI CONVECTION IN A RECTANGULAR BOX WITH SLIPPERY WALLS
Dauby, Pierre ULg; Lebon, Georgy ULg; COLINET, P. et al

in Quarterly Journal Of Mechanics And Applied Mathematics (1993), 46(Part 4), 683-707

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See detailIntracellular damages induced by singlet oxygen are signals for HIV-1 reactivation
Piette, Jacques ULg; Legrand-Poels, Sylvie ULg

in Journal of Free Radicals in Biology & Medicine (1993)

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See detailOnce daily administration of amikacin. Adaptation to neonatology for infants less than 3 days of postnatal age
Battisti, Oreste ULg

in Médecine et Maladies Infectieuses (1993), 23

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See detailSmall Bowel Enema and Diagnosis of Chronic Nonischemic Disturbance of Superior Mesenteric Venous Blood Flow
Boverie, J. H.; COUNET, D.; Meunier, Paul ULg et al

in Abdominal Imaging (1993), 18(3), 265-70

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis ... [more ▼]

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis and chronic thrombotic forms with portal hypertension or hypercoagulopathy. In eight patients this syndrome was secondary to organic lesions of different origin: mesenteric vein squeezed by fibrous bands or an abnormal jejunal artery (four cases), lymphoma involving the distal superior mesenteric veins (three cases), hemangioma causing microthrombi (one case). In three patients no etiology or predisposing factor was found. All patients presented with rectal hemorrhage. Small bowel enema showed a constant pattern in 11 patients: small nodules, modified by compression or peristalsis, involving the mesenteric border of the jejunoileal segment, and associated with thick, straight but regular folds. Mesenteric varices were suspected and led to angiographic studies which were normal in three cases, confirmed varices in eight cases, and thrombosis in four cases. Laparotomy was normal in three cases and established the etiological diagnosis in eight cases. Varices were shown in six cases. Arteriography and laparotomy were unable to reach a complete diagnosis. [less ▲]

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See detailVers une optimalisation de la formation langagière de futurs cadres: une expérience en semi-autonomie à la K.U.Leuven
Sanctobin, V.; Slootmaekers, A.; Vanden Berghe, Kristine ULg et al

in Association Belge de Linguistique Appliquée Papers (1993), 15

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See detailLes chimpanzés : un modèle animal de la relation clientélaire
Servais, Véronique ULg

in Terrain (1993), 21(octobre), 67-80

The paper is about the dominance and coalition relationship among male chimpanzees. It focuses on the dependance relationship between the members of the coalition and distinguishes stable and unstable ... [more ▼]

The paper is about the dominance and coalition relationship among male chimpanzees. It focuses on the dependance relationship between the members of the coalition and distinguishes stable and unstable coalitions. A coalition is unstable if the support of the dominated individual is conditional and stable if it is not (i.e. among two brothers). In an unstable coalition the dominated indivuual can afford some priviledge, the dominant is very dependent on the subordinate and the relationship with the dominant is tense. Analogies with human power relationships are suggested. [less ▲]

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See detailThe 16-kilodalton N-terminal fragment of human prolactin is a potent inhibitor of angiogenesis
Clapp, Carmen; Martial, Joseph ULg; Guzman, Raphaël C et al

in Endocrinology (1993), 133(3), 1292-9

The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable ... [more ▼]

The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of rat PRL (16K rPRL) was a potent inhibitor of capillary endothelial cell proliferation via a novel receptor. We now report that the nanomolar concentrations of recombinant human 16K PRL inhibit basal and basic fibroblast growth factor- or vascular endothelial growth factor-stimulated growth of bovine brain capillary endothelial cells. 16K human (h) PRL also inhibits stimulation of human umbilical vein endothelial cell proliferation by basic fibroblast growth factor. The organization of endothelial cells into capillary-like structures in type I collagen gels is also prevented by 16K hPRL. Furthermore, in an in vivo assay, the chick embryo chorioallantoic membrane assay, 16K hPRL as well as 16K rPRL were potent inhibitors of capillary formation. 16K hPRL, like 16K rPRL, maintains its biological activity as a partial PRL agonist at PRL receptors on mammary gland epithelial cells. These data demonstrate for the first time that the biological activity of 16K rPRL is not unique and that similar fragments of hPRL are active. The antiangiogenic activity of these molecules is conserved across avian and mammalian species. That 16K hPRL is a potent antiangiogenic factor in in vitro and an in vivo assay raises the exciting potential of this peptide being capable of inhibiting tumor growth. [less ▲]

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See detailEffects on performance and meat characteristics of a diet supplemented with fat during the finishing period of growing fattening bulls
Hollo, Véronique; Marche, Christian; Dufrasne, Isabelle ULg et al

in Animal Production (1993), 56

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See detailIdentification of elemental sulphur (S8) in Boscia senegalensis (Pers.) Lam ex Poir. leaves.
Lognay, Georges ULg; Seck, D.; Marlier, M. et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(4),

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See detailSTUDY OF THE CRYSTALLIZATION PROCESS IN BI2-XPBXSR2CA2CU3O10-Y GLASS SYSTEMS - OPTICAL POLARIZED-LIGHT MICROSCOPY, ELECTRICAL AND MAGNETIC-PROPERTIES
Cloots, Rudi ULg; Romain, Anne-Claude ULg; Rulmont, André ULg et al

in Superconductor Science & Technology (1993), 6(12), 850-857

Synthesis of Bi2-xPbxSr2Ca2Cu3O10-y (x = 0 and x = 0.3) has been performed using the glass recrystallization route technique. After splat quenching, the samples were annealed at 820, 850 or 870-degrees-C ... [more ▼]

Synthesis of Bi2-xPbxSr2Ca2Cu3O10-y (x = 0 and x = 0.3) has been performed using the glass recrystallization route technique. After splat quenching, the samples were annealed at 820, 850 or 870-degrees-C for 96 or 144 h (with one intermediary grinding). High-resolution polarization micrographs and electrical (and magnetic susceptibility) measurements have been taken. Various growth mechanisms can thus be checked, and the physical properties are studied in relation to the microstructure. It is found that the 2223 phase does not crystallize directly from the amorphous one but results from a dissolution-reprecipitation process. The stability of the 2212 phase is emphasized. The 2201 phase precipitates at high temperature and rapidly reacts with the melt to form the 2223 phase. The kinetics of the lead-substituted sample seems to be controlled by the Ca2PbO4 phase. which acts as a flux. [less ▲]

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See detailLes noms belgoromans de la rougeole. Une étude de géographie linguistique
Boutier, Marie-Guy ULg

in Dialectes de Wallonie (1993), 21/22

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See detailUne logique de l'anorexie mentale
Servais, Véronique ULg

in Revue Internationale de Systémique (1993), 7(4), 385-418

The paper presents a batesonian approach of anorexia nervosa. The illness is viewed as a positive feedback loop. The first restrictions appear as a solution to diverse problems of adolescence. Then the ... [more ▼]

The paper presents a batesonian approach of anorexia nervosa. The illness is viewed as a positive feedback loop. The first restrictions appear as a solution to diverse problems of adolescence. Then the restrictions become a deutero-learning : the girl finds pleasure and security in restriction behaviours. She si permanently looking for challenging contexts where she can proof her self control. Because the control is a deutero-learning the behavior cannot be diminished through punishment. Only the restoration of relationships with the whole personnality and the environment can give the girl the therapeutic experience of becoming to as system that she cannot control. [less ▲]

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See detailEtude comparative des formules d'interaction poutre-poteau
Jaspart, Jean-Pierre ULg; Briquet, C.; Maquoi, René ULg

in Construction Métallique (1993), n°4

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See detailCarbohydrate-deficient glycoprotein syndrome type II.
Jaeken, J.; De Cock, P.; Stibler, H. et al

in Journal of inherited metabolic disease (1993), 16(6), 1041

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See detailTHE CERTIFIED TOTAL GLUCOSINOLATE CONTENT IN 3 RAPESEEDS (CRM-190, CRM-366 AND CRM-367)
Wathelet, Jean-Paul ULg; WAGSTAFFE, P. J.; BOENKE, A. et al

in Fresenius' Journal of Analytical Chemistry (1993), 347(10-11), 396-399

The preparation of certified rapeseed reference materials and the steps taken to establish the certified values for total glucosinolate contents are described. Details are given of the studies to ... [more ▼]

The preparation of certified rapeseed reference materials and the steps taken to establish the certified values for total glucosinolate contents are described. Details are given of the studies to demonstrate the homogeneity and stability of the material. The results of the certification exercise for total glucosinolate content, which involved European laboratories who applied a variety of methods (high-pressure liquid chromatography, gas liquid chromatography, determination of enzymatically released glucose and sulphate), are presented and discussed. The certified values for the glucosinolate content (mumol/g of non-defatted whole seeds) and the 95% confidence level are the following: CRM 366 (12.1 +/- 0.8 mumol/g), CRM 190 (25.5 +/- 0.9 mumol/g) and CRM 367 (102.4 +/- 3 mumol/g) (CRM = certified reference material). Indicative values (not certified) for individual glucosinolates are also given. Certified sulphur values (mg/g of non defatted whole seeds) have been determined by other laboratories: CRM 366 (3.41 +/- 0.12 mg/g), CRM 190 (4.93 +/- 0.15 mg/g) and CRM 367 (10.5 +/- 0.40 mg/g). [less ▲]

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See detailFurther studies on the genetic diversity of lima beans (Phaseolus lunatus L.) using allozymes
Maquet, Alain; Wathelet, Bernard ULg; Baudoin, Jean-Pierre ULg

in Annual Report of the Bean Improvment Cooperative (1993), 36

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See detailUltrastructural distribution of DNA within the nucleolus of various animal cell lines or tissues revealed by terminal deoxynucleotidyl transferase.
Thiry, Marc ULg; Ploton, D.; Menager, M. et al

in Cell & Tissue Research (1993), 271(1), 33-45

We have used the highly sensitive in situ terminal deoxynucleotidyl transferase method, applied to ultrathin sections, to investigate the location of DNA within nucleoli of various animal cells. In all ... [more ▼]

We have used the highly sensitive in situ terminal deoxynucleotidyl transferase method, applied to ultrathin sections, to investigate the location of DNA within nucleoli of various animal cells. In all the nucleoli studied, intense labelling is revealed over the peri- and intranucleolar condensed chromatin. Gold particles are also consistently found over the fibrillar centres, especially at their periphery, namely in the border area between the fibrillar centres and the dense fibrillar component, whereas the dense fibrillar component itself seems to be free of label in nucleoli in which these two compartments can be distinguished. We conclude that, in transcriptionally active nucleoli of this type, DNA is a characteristic constituent of the fibrillar centres, distinguishing them functionally from the dense fibrillar component. Some nucleoli exhibit neither fibrillar centres nor a dense fibrillar component, but have a single, albeit heterogeneous accumulation of fibrillar material; gold particles are consistently seen over some parts of this fibrillar compartment. This suggests that certain parts of the fibrillar material are functionally similar to the fibrillar centres of those nucleoli that possess them. [less ▲]

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See detailRéussite de quatre nichées successives chez le martin pêcheur d'Europe (Alcedo atthis) au cours de la même saison
Libois, Roland ULg

in Aves (1993), 30(1), 31-36

From 1985 to 1992, 229 breeding pairs of kingfisher were studied to determine the reproduction success and the way population recovered after the severe winter 1984-85. From these investigations, we were ... [more ▼]

From 1985 to 1992, 229 breeding pairs of kingfisher were studied to determine the reproduction success and the way population recovered after the severe winter 1984-85. From these investigations, we were able to observe four successive nesting attempts by five different pairs. Four broods were raised by one of the pairs. As the adult were ringed birds and were regularky checked throughout the breeding season, ther can be no doubt, now, as to the possibility of a fourth brood for this species, at least in peculiar circumstances. [less ▲]

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See detailNote sur les techniques de gravure des sceaux-cylindres en Asie antérieure
Tunca, Önhan ULg

in Technologie et analyse des gemmes anciennes (PACT 23) (1993)

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See detailStructure, function and assembly of the nucleolus.
Scheer, U.; Thiry, Marc ULg; Goessens, Guy ULg

in Trends in Cell Biology (1993), 3(7), 236-41

Most events of ribosome biogenesis--such as transcription of the ribosomal RNA (rRNA) genes, processing of their primary transcripts into mature rRNAs and assembly with ribosomal and nonribosomal proteins ... [more ▼]

Most events of ribosome biogenesis--such as transcription of the ribosomal RNA (rRNA) genes, processing of their primary transcripts into mature rRNAs and assembly with ribosomal and nonribosomal proteins to form the preribosomes--are confined to a special nuclear compartment, the nucleolus. Immunogold labelling and in situ hybridization at the ultrastructural level are providing novel insights into structure-function relationships of the nucleolus, and in vitro systems are beginning to shed light on the molecular mechanisms involved in the reforming of nucleoli after mitosis. [less ▲]

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See detailEssential oil production by Anthemis nobilis L. tissue culture.
Fauconnier, Marie-Laure ULg; Jaziri, M.; Marlier, Michel et al

in Journal of Plant Physiology (1993), 141(6), 759-761

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was ... [more ▼]

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was compared with that of plants grown in a field. The addition of crude polysaccharide fraction prepared from yeast extract and from the plant itself to shoot cultures affected the composition and total essential content (from 0.08 o/o to 0.3Qo/o dry weight). The essential oil content of the crown-gall tissue ol A. nobilis was 0.25 % of the dry weight and the composition of the essential oil was comparable to that of the flowers. [less ▲]

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See detailDetection of ribosomal RNA during the cell cycle in Ehrlich tumour cells by electron microscope in situ hybridization.
Thiry, Marc ULg

in European Journal of Morphology (1993), 31(1-2), 13-6

The fine spatial distribution of rRNA within Ehrlich tumour cells has been investigated during the cell cycle by in situ hybridization with a biotinylated probe. In interphase cells, rRNA was essentially ... [more ▼]

The fine spatial distribution of rRNA within Ehrlich tumour cells has been investigated during the cell cycle by in situ hybridization with a biotinylated probe. In interphase cells, rRNA was essentially detected in the ribosome-rich cytoplasmic regions and in the three main nucleolar components. In mitotic cells, besides the presence, as expected, of rRNA in the ribosome-rich spaces, evident label was observed over material situated around and between the chromosomes during anaphase and telophase as well as over the reforming nucleoli during telophase. [less ▲]

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See detailDetection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by PCR in the worm nereis diversicolor
Salzet-Raveillon, B.; Rentier-Delrue, Françoise ULg; Dhainaut, A.

in Cellular and Molecular Biology (1993)

Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment (1-33th) and an 43 amino acids long internal fragment, two oligonucleotides primers were ... [more ▼]

Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment (1-33th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated. [less ▲]

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See detailUltrastructural distribution of DNA and RNA within the nucleolus of human Sertoli cells as seen by molecular immunocytochemistry.
Thiry, Marc ULg

in Journal of Cell Science (1993), 105 ( Pt 1)

The precise distribution of DNA and RNA within the human Sertoli cell nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. In Sertoli ... [more ▼]

The precise distribution of DNA and RNA within the human Sertoli cell nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. In Sertoli cells, the nucleolar components show a typical spatial distribution. The fibrillar centres are not surrounded by a layer of dense fibrillar component, but come in contact only with strands of dense fibrillar component. These fibrillar parts of strands are the extensions of granular strands connected to a large granular mass. These strands delimit numerous nucleolar interstices in which chromatin fibres are clearly obvious. Using the in situ terminal deoxynucleotidyl transferase/immunogold procedure for detecting DNA, we find evident label exclusively over the chromatin fibres enclosed in the nucleolar interstices and over the fibrillar centres, and no significant label over the dense fibrillar component and granular component of the nucleolus. Furthermore, using the polyadenylate nucleotidyl transferase/immunogold procedure for detecting RNA, we show that label is deposited not only over the granular component and dense fibrillar component, as expected, but also quite obviously over the fibrillar centres. No label is seen over the interstices containing chromatin. [less ▲]

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See detailCHARACTERIZATION OF 3 LABDANE DITERPENES FROM AFRAMOMUM-ALBOVIOLACEUM
Marlier, Michel; LEGUELLEC, G.; Lognay, Georges ULg et al

in Planta Medica (1993), 59(5), 455-457

Two labdane diterpenes were isolated from the seeds and the rhizomes of Aframomum alboviolaceum (Ridley) K. Schum (Zingiberaceae) and identified by GC-MS, H-1-, and C-13-NMR as (E)-labda-8(17),12-diene-15 ... [more ▼]

Two labdane diterpenes were isolated from the seeds and the rhizomes of Aframomum alboviolaceum (Ridley) K. Schum (Zingiberaceae) and identified by GC-MS, H-1-, and C-13-NMR as (E)-labda-8(17),12-diene-15,16-dial (1) and (E)-8beta,17-epoxylabd-12-ene-15,16-dial (2). A third minor compound could be the methyl (E)-14xi,15-epoxylabd-8(17), 12-dien-16-oate. The simultaneous occurrence of these three molecules has been mentioned only in one other species of the same genus, Aframomum daniellii (1). [less ▲]

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See detailLes méthodes d'analyse factorielle : principes et applications
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1993), 1

This note describes in a simply way the principles of the factorial methods, and more particularly of the principal component analysis and the correspondence analysis. The emphasis is put on elements ... [more ▼]

This note describes in a simply way the principles of the factorial methods, and more particularly of the principal component analysis and the correspondence analysis. The emphasis is put on elements useful when interpreting the results of an analysis and the methods are illustrated by completely worked out examples. [less ▲]

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See detailBiphasic locomotor effects of the dopamine D-sub-1 agonist SKF 38393 and their attenuation in non-habituated mice
Tirelli, Ezio ULg; Terry, P.

in Psychopharmacology (1993), 110(1-2), 69-75

Examined the locomotor stimulatory effects of the dopamine D-sub-1 receptor partial agonist SKF 38393 in male C57B1/6J mice. Non-habituated mice showed marked dose-related (3-300 mg/kg, subcutaneously ... [more ▼]

Examined the locomotor stimulatory effects of the dopamine D-sub-1 receptor partial agonist SKF 38393 in male C57B1/6J mice. Non-habituated mice showed marked dose-related (3-300 mg/kg, subcutaneously) locomotor stimulation. The time-course effect was biphasic at very high doses (100-300 mg/kg), with dose-related locomotor depression followed by dose-related long-term hyperlocomotion. For all doses, locomotor effects were detectable throughout the 4-hr test period. To determine whether these effects were mediated by D-sub-1 receptor stimulation, effects of SKF 38393 were assessed in combination with behaviorally inactive and active doses (0.1 and 0.2 mg/kg, respectively) of the selective D-sub-1 receptor antagonist SCH 39166. Both doses of SCH 39166 attenuated the hyperlocomotion induced by 30 mg/kg of the agonist to a similar degree. However, neither dose was able to reverse either the depressant or the stimulatory effects of 300 mg/kg SKF 38393. ((c) 1997 APA/PsycINFO, all rights reserved) [less ▲]

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See detailFollicular Dendritic Cells Do Not Produce Tnf-Alpha nor its Receptor
Mancini, I.; Bosseloir, A.; Hooghe-Peters, E. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailMeasurement of the 2s2p2(4P)3s 3P2 lifetime in N II by beam-foil-laser spectroscopy
Bastin, Thierry ULg; Baudinet-Robinet, Y.; Garnir, Henri-Pierre ULg et al

in Journal Physical II (1993), 3

An accurate value for the radiative lifetime of the 2s2p2(4P)3s3P2 level in N II has been obtained using the beam-foil-laser spectroscopy method. The result (T = 0.456 ± 0.020 ns ) is shorter than the ... [more ▼]

An accurate value for the radiative lifetime of the 2s2p2(4P)3s3P2 level in N II has been obtained using the beam-foil-laser spectroscopy method. The result (T = 0.456 ± 0.020 ns ) is shorter than the beam-foil lifetime values reported previously for the 3s' 3P term. No theoretical value for the lifetime of this term is available at the present time. [less ▲]

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See detailMetabolism of Thiamine Triphosphate in Rat Brain: Correlation with Chloride Permeability
Bettendorff, Lucien ULg; Peeters, Maryline; Wins, Pierre et al

in Journal of Neurochemistry (1993), 60(2), 423-434

Our results show that a net synthesis of thiamine triphosphate (TTP) can be demonstrated in vitro using rat brain extracts. The total homogenate was preincubated with thiamine or its diphosphate ... [more ▼]

Our results show that a net synthesis of thiamine triphosphate (TTP) can be demonstrated in vitro using rat brain extracts. The total homogenate was preincubated with thiamine or its diphosphate derivative (TDP), centrifuged, and washed twice. With TDP (1 mM) as substrate, a 10-fold increase in TTP content was observed in this fraction (nuclear fraction, membrane vesicles). A smaller, but significant, increase was observed in the P2 fraction (mitochondrial/synaptosomal fraction). In view of the low TTP content of our fractions, it was carefully assessed that authentic TTP was being formed. Incorporation of radioactivity from [beta-32P]TDP and [gamma-32P]ATP in TTP suggests that these two compounds are its precursors. Furthermore, TTP synthesis was inhibited by ADP and relatively low concentrations of Zn2+. These results suggest that TTP synthesis is catalyzed by an ATP:TDP transphosphorylase rather than by the cytoplasmic adenylate kinase that may be present in the vesicles. After osmotic lysis of the vesicles at alkaline pH, TTP was recovered in protein-bound form. Concomitantly, a soluble thiamine triphosphatase, with alkaline pH optimum, was also released from the vesicles. No net synthesis could be obtained in the cytosolic fraction or in detergent-solubilized systems. Like TTP synthesis, chloride permeability of the vesicles was increased when the homogenate had been incubated with thiamine and particularly with TDP. Our results suggest a regulatory role of TTP on chloride permeability, but the target remains to be characterized. [less ▲]

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See detailCalcitonins: Newer Routes of Delivery
Reginster, Jean-Yves ULg

in Osteoporosis International : A Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis & the National Osteoporosis Foundation of the USA (1993), 3(Suppl 2), 3-7

Because of its anti-osteoclastic and analgesic properties, calcitonin is a first-line choice in the treatment of several bone diseases characterized by absolute or relative bone resorption. With regard to ... [more ▼]

Because of its anti-osteoclastic and analgesic properties, calcitonin is a first-line choice in the treatment of several bone diseases characterized by absolute or relative bone resorption. With regard to the prevention and treatment of postmenopausal osteoporosis, the chronic nature of the disease and the subsequent long duration of the pharmacological intervention require uncomfortable parenteral administration to be repeated on a long-term basis. Newer routes of administration have therefore been developed. At present the most promising of these are the nasal spray and the rectal suppository. Both routes have been shown to induce significant increases in plasma levels of salmon calcitonin. The ability of salmon calcitonin to cross the nasal mucosa was shown immunologically by the generation of specific anti-salmon-calcitonin antibodies in the plasma. In healthy volunteers these alternative routes of administration do not decrease the anti-osteoclastic activity of calcitonin, as demonstrated by a significant decrease in biochemical parameters that reflect bone turnover. In the prevention and treatment of postmenopausal osteoporosis, as well as in the treatment of Paget's disease of bone, the properties of calcitonins are generally preserved if they are administered non-parenterally. However, the equivalent doses required to sustain the effects of calcitonin have not been fully elucidated. This article reviews the potential benefits of two methods of non-parenteral administration of calcitonins in the prevention and treatment of postmenopausal osteoporosis. [less ▲]

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See detailLes formes normes d’ordres,
Pays, Isabelle ULg

in Journal of Algebra (1993), 155

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See detailAssociation of Aortic Stenosis and Gastrointestinal Bleeding (Heyde's Syndrome). Report of Two Cases
Natowitz, L.; DEFRAIGNE, Jean ULg; Limet, Raymond ULg

in Acta Chirurgica Belgica (1993), 93(1), 31-3

The association between aortic stenosis and digestive angiodysplasia has been described for the first time by Heyde in 1958. This entity is thus known as Heyde's syndrome. In many instances, the recurrent ... [more ▼]

The association between aortic stenosis and digestive angiodysplasia has been described for the first time by Heyde in 1958. This entity is thus known as Heyde's syndrome. In many instances, the recurrent small intestinal bleeding originating from angiodysplasia stopped after aortic valve replacement. We report two cases of patients presenting with a recurrent small intestinal bleeding originating from digestive angiodysplasia and suffering from aortic stenosis. Diagnosis of both pathologies is well documented in both cases. The replacement of the aortic valve by a biologic prosthesis stopped the bleeding. Prior to aortic valve replacement, the two patients suffered severe recurrent blood loss from intestinal angiodysplasia. The treatment of aortic stenosis greatly favored both cardiac and general status. We recommend aortic valve replacement with a biologic prosthesis prior to intestinal resection in patients presenting with Heyde's syndrome. We stress on the fact that anticoagulants must be stopped in order to minimize the risk of further bleeding. [less ▲]

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See detailProduction of active oxygen species by isolated human chondrocytes.
Henrotin, Yves ULg; Deby-Dupont, G.; DEBY, C. et al

in British Journal of Rheumatology (1993), 32(7), 562-7

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric ... [more ▼]

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric method (production of dichlorofluorescein from a precursor in the presence of horseradish peroxidase and H2O2) and by a chromatographic method (measurement of ethylene production from gamma-methiol-keto-butyric acid after .OH attack). Chondrocytes were tested, both with and without activation by phorbol myristate acetate (PMA: 10(-6) M), in the presence of Ca2+ (1 x 10(-4) M) and Mg2+ (2 x 10(-4) M) or after variable periods of anoxia under nitrogen (4 to 12 h) followed by reoxygenation (with 95% O2, 5% CO2). Under these experimental conditions, the PMA-excited chondrocytes produced from 80 to 180 nmol of hydrogen peroxide per 1 x 10(6) cells and chondrocytes subjected to anoxia-reoxygenation produced up to 1700 nmol H2O2 per 1 x 10(6) cells. The hydroxyl radical production by PMA or anoxia-reoxygenation excited cells reached 600% of the production of non-excited cells and 1300% when they were subjected to successive stimulations by PMA and anoxia-reoxygenation. The possible pathological significance of these observations is discussed. The results indicate that stimulated human chondrocytes are capable of producing active oxygen species which could play a major role in joint inflammation and cartilage damage. [less ▲]

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See detailConnection and frame design for economy
Anderson, D.; Colson, A.; Jaspart, Jean-Pierre ULg

in New Steel Construction (1993), 1(N°6), 30-33

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See detailExemples d'utilisation de la Carte des Sols de Belgique comme base d'un systeme d'information geographique pour la gestion des terroirs.
Engels, Patrick ULg; Bock, Laurent ULg; Neven, Cécile et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(2-3),

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See detailAnti-Ia treatment prevents lupus-like autoimmune syndrome in mice neonatally tolerized to alloantigens.
Kramar, G.; Schurmans, Stéphane ULg; Aguado, T. et al

in Journal of Autoimmunity (1993), 1

Neonatal injection of semi-allogeneic F1 spleen cells into newborn parental mice results in induction of tolerance to the corresponding class I alloantigen and chimerism. This state of tolerance is ... [more ▼]

Neonatal injection of semi-allogeneic F1 spleen cells into newborn parental mice results in induction of tolerance to the corresponding class I alloantigen and chimerism. This state of tolerance is associated with the development of a transient lupus-like autoimmune syndrome. Previous experiments performed in our laboratories have shown that host CD4+ T lymphocytes and donor B cells persist in the host and are essential in triggering the autoimmune syndrome observed in neonatally tolerized mice. In this study, we show that early treatment of tolerized mice with anti-donor MHC class II mAb totally prevents the lupus-like syndrome. Moreover, delayed treatment significantly decreases, but to a lesser extent, autoimmune pathological features in tolerized mice. Taken together, these results show that lupus-like autoimmune syndrome developed by neonatally tolerized mice is efficiently prevented by anti-Ia treatment without interfering with the induction of tolerance [less ▲]

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See detailMeasurement of Total Respiratory Impedance in Dogs by the Forced Oscillation Technique
Clercx, Cécile ULg; Gustin, Pascal ULg; Landser, F. J. et al

in Veterinary Research Communications (1993), 17(3), 227-239

The resistance (Rrs) and reactance (Xrs) of the total respiratory system were determined at various frequencies in 14 healthy conscious beagle dogs. A pseudorandom noise pressure wave was produced at the ... [more ▼]

The resistance (Rrs) and reactance (Xrs) of the total respiratory system were determined at various frequencies in 14 healthy conscious beagle dogs. A pseudorandom noise pressure wave was produced at the nostrils of the animals by means of a loudspeaker adapted to the nose by a tightly fitting mask. A Fourier analysis of the pressure and flow signals yielded mean Rrs and Xrs, over 16 s, at frequencies from 2 to 26 Hz. The influence of the posture of the dog, the position of its head, the linearity of the respiratory system, the reproducibility of the method and the effects of upper and lower airway obstructions were studied. In sitting and standing healthy dogs with the head in the extended position, Rrs values increased progressively with frequency from 5.4 +/- 0.4 (SEM) cmH2O L-1s at 6 Hz up to 8.8 +/- 0.7 cmH2O L-1s at 26 Hz, the mean resonant frequency being 6.1 +/- 0.5 Hz. No significant differences were observed between measurements performed with the head in the normal or the extended position. In a recumbent posture, all Rrs values were increased but Rrs was still dependent on the frequency in the same way (7.1 +/- 0.7 cmH2O L-1s at 6Hz up to 10.0 +/- 0.5 cmH2O L-1s at 26 Hz). Tracheal compression also induced higher Rrs values without changes in the frequency dependence or in the resonant frequency. In anaesthetized dogs, airway obstruction was induced by inhalation of histamine (4 mg/ml for 5 min; the Rrs values tended to decrease with increasing frequency, and the resonant frequency was markedly increased [less ▲]

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See detailEvolution de la situation des mammifères sauvages en Région wallonne au cours de décennie 1983-1992
Libois, Roland ULg

in Cahiers d'Ethologie (1993), 13(1), 77-92

During the last ten years, the evolution of the status of the mammal fauna of Wallony (southern part of Belgium) can be characterized by four main trends: i- enforcement of a better legal protection ii ... [more ▼]

During the last ten years, the evolution of the status of the mammal fauna of Wallony (southern part of Belgium) can be characterized by four main trends: i- enforcement of a better legal protection ii- arrival of at least four new species: coypu, raccoon, raccon-dog and American mink iii- restoration of badger numbers following the efficient control of rabies iv- increase of anthropophilic or game species (stone marten, ungulates). Unfortunately, the most threathened species (otter, horseshoe bats, barbastella) did not recover. [less ▲]

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See detailModifications of the extracellular matrix of aneurysmal abdominal aortas as a function of their size.
SAKALIHASAN, Natzi ULg; HEYERES, Antoine ULg; Richelle, Betty ULg et al

in European Journal of Vascular Surgery (1993), 7(6), 633-7

Collagen and elastin are the main extracellular matrix proteins providing the aortic wall with adequate mechanical properties and resistance for proper function. Our study aimed at investigating the ... [more ▼]

Collagen and elastin are the main extracellular matrix proteins providing the aortic wall with adequate mechanical properties and resistance for proper function. Our study aimed at investigating the relationship between the elastin concentration of the wall of normal and aneurysmal abdominal aortas (AAA), the collagen concentration, and its extractability, as a function of their size. Infrarenal aortas were collected from 30 patients undergoing operative repair of abdominal aortic aneurysm. Age-matched control samples were obtained from eight autopsies of individuals without vascular disease. Samples were divided into five groups according to the aortic diameter: control group (group N, n = 8); < 50 mm (group I, n = 6; between 50-75 mm (group II, n = 10); > 75 mm (group III, n = 7); and ruptured (group IV, n = 7). The collagen concentration in samples from group I was similar to the controls. An increased collagen concentration was observed in group II and remained at the same level in the largest and ruptured aneurysms. Extractability of collagen was found to be increased in group III and was even higher in group IV. A highly significant reduction in elastin concentration was observed in group I and there was progressive reduction with increasing diameter and rupture. A significant correlation could be established between aortic diameter, increased collagen extractability and decreased elastin content. [less ▲]

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See detailLa place de la musique en hôpital de jour
Triffaux, Jean-Marc ULg; Bertrand, Jean ULg

in Psychologie Médicale (1993)

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See detailEvidence that insulin-like growth factor 2 (IGF2) is the dominant thymic peptide of the insulin superfamily
Geenen, Vincent ULg; Achour, Imane; Robert, Françoise et al

in Thymus (1993), 21(2), 115-127

Central T-cell tolerance of neurondocrine functions has been proposed to be primarily induced by the thymic repertoire of neuroendocrine self antigens. The present study aimed at characterizing the human ... [more ▼]

Central T-cell tolerance of neurondocrine functions has been proposed to be primarily induced by the thymic repertoire of neuroendocrine self antigens. The present study aimed at characterizing the human thymic insulin-related self antigen able to represent the pancreatic islet ß cell function in face of the developing T cells. Immunofluorescence studies were performed on human and rat thymic sections, as wess as on the rat IT-45R1 thymic epithelial cell line using several antibodies to epitopes of the insulin peptide family. These studies identify beyond any doubt that IGF2 is the dominant thymic peptide of the insulin family. The sequence of an insulin-derived autoantigen is proposed. This autoantigen is a nonamer and has a hydrophobic residue leucine at position 9. In human species, this autoantigen would primarily be tolerogenic for the pancreatic ß-cell endocrine function during fetal development. [less ▲]

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See detailOptical imaging of the gravitational lens system B 1422+231
Remy; Surdej, Jean ULg; Smette, A. et al

in Astronomy and Astrophysics (1993), 278

Direct optical images (Bessel V, R and Gunn i; average seeing of 0.88 seconds) of B1422+231, obtained in March 1993 at European Southern Observatory (ESO) (La Silla), have been decomposed by fitting ... [more ▼]

Direct optical images (Bessel V, R and Gunn i; average seeing of 0.88 seconds) of B1422+231, obtained in March 1993 at European Southern Observatory (ESO) (La Silla), have been decomposed by fitting multiple Point Spread Functions (PSFs). We detect the optical counterparts of the four known radio point-like components. No additional optical image is found, but due to the compactness of the configuration, our detection limit for additional point sources is rather low (19.3 in R). The deduced positions and photometry of the four optical components agree pretty well with the radio and IR observations and this study provides further evidence supporting the gravitational lens origin of B1422+231. [less ▲]

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See detailNucleotide sequence of the bovine viral diarrhoea virus Osloss strain: comparison with related viruses and identification of specific DNA probes in the 5' untranslated region
De Moerlooze, L.; Lecomte, C.; Brown-Shimmer, S. et al

in Journal of General Virology (The) (1993), 74

The nucleotide sequence of the cytopathic Osloss isolate of bovine viral diarrhoea virus (BVDV) was deduced from overlapping cDNA clones and from PCR products. The Osloss genome is an RNA molecule of ... [more ▼]

The nucleotide sequence of the cytopathic Osloss isolate of bovine viral diarrhoea virus (BVDV) was deduced from overlapping cDNA clones and from PCR products. The Osloss genome is an RNA molecule of positive polarity containing 12480 nucleotides and having the capacity to code for a polyprotein of 3975 amino acids. The presence of the previously described internal stop codon in this viral sequence was disproved after direct sequencing of the appropriate PCR-amplified fragment. Except for the previously reported insertion of a sequence coding for a ubiquitin-like protein, the viral genome shares great similarity with those of three other strains of the pestivirus genus. Computer-assisted sequence analyses and comparisons of known pestiviral genomic sequences led us to identify selected PCR primers in the 5' untranslated region. These primers were used successfully to amplify 18 distinct pestivirus isolates and potential DNA probes were noted from the deduced sequences. The possible use of a well conserved 26 base fragment as a diagnostic probe was confirmed in hybridization experiments. The 5' untranslated region was further studied and compared with those of other members of the Flaviviridae family, which includes the flaviviruses and the hepatitis C virus group. These sequence analyses support the possibility of discrimination amongst the closely related ruminant pestiviruses, border disease virus and BVDV. [less ▲]

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See detailSequencing of a 52 kb yeast DNA located on chromosome XI of Saccharomyces cerevisiae and preliminary study of a new essential ORF.
Bolle, Paul André; Vandenbol, Micheline ULg; Portetelle, Daniel ULg et al

in Archives Internationales de Physiologie et de Biochimie (1993)

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See detailStimulation of the 92-Kd Type Iv Collagenase Promoter and Enzyme Expression in Human Melanoma Cells
Lauricella-Lefebvre, M. A.; Castronovo, Vincenzo ULg; Sato, H. et al

in Invasion & Metastasis (1993), 13(6), 289-300

The 92-kD type IV collagenase is a member of the metalloproteinase family which degrades type IV collagen, a major component of basement membrane and is involved in tumor invasion and metastasis. The ... [more ▼]

The 92-kD type IV collagenase is a member of the metalloproteinase family which degrades type IV collagen, a major component of basement membrane and is involved in tumor invasion and metastasis. The promoter and adjacent regulatory sequences of the 92-kD type IV collagenase have been identified previously and three cis-acting elements homologous to the binding sites for AP-1, NF-KB and SP-1 proteins contributed to induction of the promoter activity by 12-O-tetradecanoylphorbol 13-acetate (TPA) and tumor necrosis factor (TNF-alpha) in HT1080 cells. To date, no direct correlation between promoter activity and expression of the 92-kD type IV collagenase has been reported in normal or cancer cells. In this study, the effects of the transcriptional stimulation of the 92-kD type IV collagenase gene on the expression of the enzyme in human A2058 melanoma cells was analyzed by zymography experiments. Quantitative immunoblots using a monoclonal antibody that recognized specifically and exclusively the 92-kD type IV collagenase, confirmed that the 92-kD gelatinase was 92-kD type IV collagenase. Stimulation of the promoter activity resulted in increased gelatinase activity in the culture medium of A2058 cells. A direct correlation between TPA- and TNF-alpha-mediated promoter stimulation of the 92-kD type IV collagenase gene and its expression was also demonstrated in the human fibrosarcoma HT1080 cells. Interleukin-1 alpha failed to induce 92-kD gene promoter activity and type IV collagenase expression in melanoma and fibrosarcoma cell lines. Our data demonstrated that TPA- and TNF-alpha-induced 92-kD type IV collagenase promoter stimulation leads to a proportional increase of enzyme expression and secretion and thus could contribute to the activation of the invasive phenotype. [less ▲]

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See detailReplacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme
Mainfroid, Véronique; Goraj, Karine; Rentier-Delrue, Françoise ULg et al

in Protein Engineering (1993), 6(8), 893-900

In order to investigate how structural modifications interfere with protein stability, we modified a (beta alpha)-unit in E.coli triosephosphate isomerase (TIM), a typical (beta alpha)-barrel protein ... [more ▼]

In order to investigate how structural modifications interfere with protein stability, we modified a (beta alpha)-unit in E.coli triosephosphate isomerase (TIM), a typical (beta alpha)-barrel protein, assuming that the pseudosymmetrical beta-barrel can be divided into eight successive loop/beta-strand/loop/alpha-helix motifs. We replaced the eighth (beta alpha)-unit of E.coli TIM with the corresponding chicken (beta alpha)-unit. The substitution, involving the replacement of 10 of the 23 residues of this (beta alpha)-unit, was evaluated first by modelling, then experimentally. Modelling by homology suggests how the amino acid replacements might be accommodated in the hybrid E.coli/chicken TIM (ETIM8CHI). Both natural and hybrid recombinant TIMs, overproduced in E.coli, were purified to homogeneity and characterized as to their stability and kinetics. Our kinetic studies show that the modification performed here leads to an active enzyme. The stability studies indicate that the stability of ETIM8CHI is comparable to that of the wild type TIM. [less ▲]

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See detailEpidural anesthesia and intrathoracic blood volume.
Warner, David O; Brichant, Jean-François ULg; Ritman, Erik L et al

in Anesthesia and Analgesia (1993), 77(1), 135-40

To investigate how epidural anesthesia changes intrathoracic gas volume, high epidural anesthesia was administered to five pentobarbital-anesthetized dogs lying supine, and the total intrathoracic volume ... [more ▼]

To investigate how epidural anesthesia changes intrathoracic gas volume, high epidural anesthesia was administered to five pentobarbital-anesthetized dogs lying supine, and the total intrathoracic volume at end-expiration was measured with a high-speed three-dimensional x-ray scanner. The amount of gas in the lungs at end-expiration [the functional residual capacity (FRC)] was measured with a nitrogen washout technique, and the intrathoracic tissue volume, including the intrathoracic blood volume, was calculated as the difference between intrathoracic volume at end-expiration and FRC. High epidural anesthesia with the local anesthetic etidocaine to a T-1 myotomal level significantly (P < 0.05) increased intrathoracic volume at end-expiration [76 +/- 35 mL (M +/- SD)] by significantly increasing both intrathoracic tissue volume (33 +/- 15 mL) and FRC (43 +/- 26 mL). Increases in intrathoracic tissue volume were probably caused by increases in intrathoracic blood volume. We conclude that increases in FRC caused by epidural anesthesia in anesthetized dogs lying supine may be minimized by a concurrent increase in intrathoracic blood volume. These results suggest that measurements of thoracic gas volume alone may be insufficient to describe chest wall responses to epidural anesthesia. [less ▲]

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See detailCHARACTERIZATION OF A HIGHLY PHOTOACTIVE MOLECULAR SEMICONDUCTOR - OXOTITANIUM PHTHALOCYANINE
Ghosez, Philippe ULg; COTE, R.; GASTONGUAY, L. et al

in Chemistry of Materials (1993), 5(10), 1581-1590

Oxotitanium phthalocyanine (OTiPc) thin films have been sublimed at various thicknesses on SnO2 and glass substrates. The morphology, crystallinity, and photoelectrochemical activity of the films have ... [more ▼]

Oxotitanium phthalocyanine (OTiPc) thin films have been sublimed at various thicknesses on SnO2 and glass substrates. The morphology, crystallinity, and photoelectrochemical activity of the films have been studied. It was found that the physical and photoelectrochemical properties of the films are greatly influenced by the temperature reached by the substrate during the sublimation. Below 80-degrees-C, amorphous films are obtained while the films are partially crystalline when the substrate is allowed to reach about 140-degrees-C. Amorphous films are made of tightly packed aggregates of circular section while partially crystalline films consists of platelets. All films are porous and permeable to the I3-/I- redox system. The dominant polymorph in partially crystalline films is not the same for all film thicknesses. It is phase IV OTiPc (as deduced by electron diffraction) for films thinner than about 2000 angstrom. On the other hand, for films thicker than about 8000 angstrom, phase I OTiPc becomes the dominant polymorph (as deduced by X-ray diffraction). It is replaced by phase II for 20 000-angstrom-thick films of OTiPc. Partially crystalline films are the only ones to absorb in the near-infrared (NIR) region. This typical absorption is going along with an improvement of the photoactivity of the films. In partially crystalline films, energy is transferred from the amorphous to the crystalline regions where most of the charges are generated. Quantum yields for electron collection per incident photon may reach over 25% in short circuit conditions, at 850 nm, the Q-band absorption maximum for 8000-angstrom-thick films. Those films are phase I OTiPc. Under 35 MW CM-2 polychromatic illumination, the same films are characterized by short circuit photocurrents of 1.5 mA cm-2. A NIR absorbance is an important factor required to obtain a high photoactivity, but it is not the only one. Interaction of OTiPc with oxygen at the purification level of the crude material is very important as well. [less ▲]

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See detailCreativity in Non-Literary Translation
Pagnoulle, Christine ULg

in Perspectives : Studies in Translatology (1993), (1), 79-90

Three short non literary texts illustrate the need for translators' actively stepping in. Since the author is both a translator and a teacher of translation didactic conclusions are also drawn.

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See detailEvolutionary relationships among higher fungi inferred from small ribosomal subunit RNA sequence analysis
Wilmotte, Annick ULg; Van De Peer, Yves; Goris, Anne et al

in Systematic & Applied Microbiology (1993), 16

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The ... [more ▼]

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The sequences were fitted into an alignment of all hitherto published complete or nearly complete eukaryotic small subunit rRNA sequences. The evolutionary relationships within the fungi were examined by construction of a tree from 87 SSU rRNA sequences, corresponding to 71 different species, by means of a distance matrix method and bootstrap analysis. It confirms the early divergence of the zygomycetes and the classical division of the higher fungi into basidiomycetes and ascomycetes. The basidiomycetes are divided into true basidiomycetes and ustomycetes. Within the ascomycetes, the major subdivisions hemiascomycetes and euascomycetes can be recognized. However, Schizosaccharomyces pombe does not belong to the cluster of the hemiascomycetes, to which it is assigned in classical taxonomic schemes, but forms a distinct lineage. Among the euascomycetes, the plectomycetes and the pyrenomycetes can be distinguished. Within the hemiascomycetes, the polyphyly of genera like Pichia or Candida and of families like the Dipodascaceae and the Saccharomycetaceae can be observed. [less ▲]

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See detailA VARIABLE MECHANISM FOR THE NUCLEOPHILIC VINYLIC SUBSTITUTIONS IN A SERIES OF GEM-DIHALOGENATED ALKENES BY A BIDENTATE SULFUR NUCLEOPHILE - AN EXPERIMENTAL AND AM1 THEORETICAL-STUDY
Gimbert, Yves; Moradpour, Alec; Dive, Georges ULg et al

in Journal of Organic Chemistry (1993), 58(17), 4685-4690

The nucleophilic substitutions of a series of gem-dihalogenated alkenes 3,5,7,8, and 9 (RS)2C=CX2 (X = Cl, F) with 1,2-benzenedithiolate b have been studied. Depending on the structures of the R groups ... [more ▼]

The nucleophilic substitutions of a series of gem-dihalogenated alkenes 3,5,7,8, and 9 (RS)2C=CX2 (X = Cl, F) with 1,2-benzenedithiolate b have been studied. Depending on the structures of the R groups (alkyl, saturated and unsaturated cycloalkyls, aromatic ring), the course of the reactions and the structures of the yielded products are modified. In the frame of the addition-elimination-type mechanism for these nucleophilic substitutions, the energy contents of the anionic intermediates, resulting from the additions of the nucleophile b to the unsaturated centers, is calculated at the AM1 method level. For the compounds 5, 7, 8, and 9, the calculated energies nicely corroborate the experimental results. For 3, anionic intermediates are no longer found by calculation, and a synchronous single-step substitution is strongly suggested. [less ▲]

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See detailBPDZ 44: a new and potent activator of ATP-sensitive K+ channels
Lebrun, P.; Pirotte, Bernard ULg; Antoine, M.-H. et al

in Diabetologia (1993), 36

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See detailThe evolution of atmospheric extinction at La Silla derived from measurements in the Stromgren photometric system
Sterken, C.; Manfroid, Jean ULg

in Astronomy and Astrophysics (1992), 266

We describe atmospheric extinction data obtained at the La Silla Observatory in the Stromgren photometric system. Our measurements, covering a time interval of almost fifteen years, show three episodes of ... [more ▼]

We describe atmospheric extinction data obtained at the La Silla Observatory in the Stromgren photometric system. Our measurements, covering a time interval of almost fifteen years, show three episodes of increased extinction due to airborne aerosols of volcanic origin. A small-amplitude seasonal variation and a short-timescale pseudo-periodic oscillation are also revealed. Implications on the observing procedures and photometric reduction schemes are discussed. [less ▲]

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See detailLong-term stability of classical photometry
Hensberge, H.; Manfroid, Jean ULg; Sterken, C.

in The Messenger (1992), 70

Not Available

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See detailStandard stars for the Infrared Space Observatory, ISO.
van der Bliek, N. S.; Bouchet, P.; Habing, H. J. et al

in The Messenger (1992), 70

Not Available

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See detailField Efficacy of Injectable and Pour-on Moxidectin in Cattle Naturally Infested with Psoroptes Ovis (Acarina: Psoroptidae)
Lonneux, J. F.; Losson, Bertrand ULg

in Veterinary Parasitology (1992), 45(1-2), 147-52

Field efficacy of moxidectin, a macrocyclic lactone endectocide, was evaluated in cattle naturally infested with Psoroptes ovis. Three groups of six animals were used based on parasitological and clinical ... [more ▼]

Field efficacy of moxidectin, a macrocyclic lactone endectocide, was evaluated in cattle naturally infested with Psoroptes ovis. Three groups of six animals were used based on parasitological and clinical status. Group 1 animals received one subcutaneous injection of injectable moxidectin at 0.2 mg kg-1 body weight; Group 2 animals received topically one dose of 0.5 mg kg-1 body weight of pour-on moxidectin; Group 3 individuals remained untreated as controls. Efficacy was assessed by (a) taking skin samples from each animal on Days -4, 14, 28, 42 and 56 post-treatment (PT) and observing numbers of viable mite stages and species and (b) clinical examination of animals on Days 14, 28, 42 and 56 with the percentage of affected body surface calculated for each animal on Days -4, 28 and 56. Both formulations gave 100% efficacy as no live mites were found in Groups 1 or 2, 14, 28 and 56 days after treatment. Clinical indices showed a sharp decrease in the affected body surface area from a mean of 5.48% and 6.1% on Day -4 in Groups 1 and 2, respectively, to 0% in both groups on Days 28 and 56 PT. All untreated animals remained positive until Day 28. The clinical condition of the controls worsened rapidly during the experiment (mean clinical index: 2.87% and 13.05% on Days -4 and 28, respectively) and they were given an emergency treatment on Day 28. No side-effects were observed with either formulation.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailOxytocin blocks the environmentally conditioned compensatory response present after tolerance to ethanol-induced hypothermia in mice
Tirelli, Ezio ULg; Jodogne, C.; Legros, Jean-Jacques ULg

in Pharmacology, Biochemistry & Behavior (1992), 43(4), 1263-1267

The present study tested the hypothesis that the attenuation by oxytocin of tolerance to ethanol-induced hypothermia relies upon an impairment of the putative conditioning processes underlying environment ... [more ▼]

The present study tested the hypothesis that the attenuation by oxytocin of tolerance to ethanol-induced hypothermia relies upon an impairment of the putative conditioning processes underlying environment-specific tolerance. According to the conditioning model of tolerance, such tolerance occurs because an opposite compensatory response conditioned to ethanol-paired cues attenuates ethanol's effects. Tolerance to ethanol-induced hypothermia was established to a particular environment over 4 days by injecting mice (daily) with oxytocin 2 h before ethanol, outside the colony room. As controls, other mice were injected similarly but following testing in the animal room. We found that oxytocin suppressed the conditioned compensatory response, revealed by injecting saline to every group in the tolerance-associated environment. These results suggest that oxytocin acted, at least partly, via an inhibition of the associative learning processes that facilitate tolerance development. [less ▲]

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See detailLe portage asymptomatique des Pestivirus chez les ruminants
Pastoret, Paul-Pierre ULg; Boulanger, D.; Mignon, Bernard ULg et al

in Revue Scientifique et Technique (International Office of Epizootics) (1992), 11(4), 1087-1096

Pestiviruses are enveloped single-chain ribonucleic acid viruses with a positive polarity. Pestiviruses include the viruses of classical swine fever (hog cholera), Border disease of sheep, mucosal disease ... [more ▼]

Pestiviruses are enveloped single-chain ribonucleic acid viruses with a positive polarity. Pestiviruses include the viruses of classical swine fever (hog cholera), Border disease of sheep, mucosal disease of cattle, and isolates obtained from wild animals, such as red deer (Cervus elaphus). Among ruminants, pestiviruses have developed a remarkable strategy for assuring their persistence. Through epigenetic transmission, they lead to the birth of asymptomatic carrier animals harbouring non-cytopathic variants, which become immunotolerant to the strain of virus present. The presence of a small number of asymptomatic carriers enables the virus to circulate within a herd by horizontal transmission, leading to the birth of a new generation of asymptomatic carriers. [less ▲]

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See detailModel-Based System for Automatic Target Recog- nition from Forward-Looking Laser-Radar Imagery
Verly, Jacques ULg; Delanoy, Richard L.; Dudgeon, Dan E.

in Optical Engineering : The Journal of the Society of Photo-Optical Instrumentation Engineers (1992), 31(12), 2540-2552

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See detailQuantification of skin color in patients undergoing maintenance hemodialysis
Deleixhe-Mauhin, F.; Krzesinski, Jean-Marie ULg; Rorive, Georges ULg et al

in Journal of the American Academy of Dermatology (1992), 27(6), 950-953

Background: In patients undergoing long-term hemodialysis, a peculiar hyperpigmentation develops, the intensity of which may or may not be related to the duration of treatment and use of erythropoietin ... [more ▼]

Background: In patients undergoing long-term hemodialysis, a peculiar hyperpigmentation develops, the intensity of which may or may not be related to the duration of treatment and use of erythropoietin. Objective: Our purpose was to conduct a comparative cross-sectional study of white patients with distinct diseases that modify their skin color. Methods: A reflected-light colorimeter was used to compare the color of the forehead and forearms of 61 white patients receiving hemodialysis with that of matched controls and of patients with anemia or icterus. Results: Significant differences were found in both reflectance and chromaticity between these groups of patients. Duration of hemodialysis and use of erythropoietin did not significantly influence the colorimetric measurements. Conclusion: The use of a color-reflectance meter allows precise evaluation of subtle changes in skin color and may be used to monitor several diseases. [less ▲]

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See detailUtilisation d'un S.I.G. pour la gestion des ressources forestières : exemple d'application à un inventaire forestier régional
Lejeune, Philippe ULg; Lecomte, Hugues; Prevot, Hugues ULg

in Bulletin des Recherches Agronomiques de Gembloux (1992), 28(2-3), 275-286

Le développement des Systèmes d'Information Géographiques (S.I.G.) ouvre de nouvelles perspectives dans le domaine de l'estimation des ressources forestières par voie d'inventaires, dont l'objectif ... [more ▼]

Le développement des Systèmes d'Information Géographiques (S.I.G.) ouvre de nouvelles perspectives dans le domaine de l'estimation des ressources forestières par voie d'inventaires, dont l'objectif principal est de déterminer les surfaces et les volumes sur pied par types de peuplement au sein d'une région donnée. La technique d'estimation des surfaces par comptage de points, classiquement utilisée dans le cas d'échantillonages systématiques, peut être avantageusement remplacée par la création, au sein d'un S.I.G., d'une carte numérique des peuplements forestiers, pouvant être reliée avec les données de l'inventaire au sol. Une méthodologie est proposée pour produire une telle carte au départ de photos aériennes infrarouges couleurs au 1/25 000. Le traitement de l'information géographique est réalisé à l'aide du logiciel ILWIS. La méthode a été testée sur une zone pilote de 20 000 ha de forêt. Le taux d'erreurs d'interprétation du type de peuplement est de l'ordre de 15 %. Il est inférieur à 6 % si l'on ne considère que les confusions portant sur les essences présentes. La combinaison de cette carte avec la couche contenant les placettes de l'inventaire a permis d'estimer l'ensemble des volumes sur pied par types de peuplement. [less ▲]

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See detailTwo Different Beta-Lactamase Genes Are Present in Streptomyces Cacaoi
Magdalena, Juana; Forsman, Mats; Lenzini, Mauro V et al

in FEMS Microbiology Letters (1992), 78(1), 101-5

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See detailSecretion by Overexpression and Purification of the Water-Soluble Streptomyces K15 Dd-Transpeptidase/Penicillin-Binding Protein
Palomeque-Messia, Pilar; Quittre, Valérie; Leyh-Bouille, Mélina et al

in Biochemical Journal (1992), 288(1), 87-91

Though synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-acid-residue Streptomyces K15 DD-transpeptidase/penicillin-binding protein is membrane-bound ... [more ▼]

Though synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-acid-residue Streptomyces K15 DD-transpeptidase/penicillin-binding protein is membrane-bound. Overexpression in Streptomyces lividans resulted in the export of an appreciable amount of the synthesized protein (4 mg/litre of culture supernatant). The water-soluble enzyme was purified close to protein homogeneity with a yield of 75%. It requires the presence of 0.5 M-NaCl to remain soluble. It is indistinguishable from the detergent-extract wild-type enzyme with respect to molecular mass, thermostability, transpeptidase activity and penicillin-binding capacity. [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 8. Ring-opening polymerization of ε-caprolactone initiated by primary amines and trialkylaluminum
Dubois, Philippe; Degée, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1992), 25

Whenever added with triethylaluminum, primary amines have been found to be effective initiators for epsilon-caprolactone polymerization in both toluene and THF at 40°C. The IR and NMR analysis of the ... [more ▼]

Whenever added with triethylaluminum, primary amines have been found to be effective initiators for epsilon-caprolactone polymerization in both toluene and THF at 40°C. The IR and NMR analysis of the polyester has supported a polymerization mechanism proceeding through a "coordination-insertion" pathway and the selective rupture of the acyl-oxygen bond of the monomer. The alkylaluminum activates the carbonyl group of the monomer and accordingly favors the nucleophilic addition of the amine, which is the actual initiation step. Propagation is typically a living process, and the molecular weight distribution is controlled by both AlEt3/amine molar ratio and solvent As an extension of that mechanism, diethylaluminum omega-amino alkoxide has been prepared on purpose and successfully used as an initiator for the synthesis of α,ω-dihydroxypoly(ε-caprolactone). [less ▲]

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See detailQualitative analysis of ten-dimensional lovelock cosmological models
Demaret, Jacques; De Rop, Yves ULg; Tombal, P. et al

in General Relativity & Gravitation (1992), 24

The Einstein-like field equations obtained from the variation of a Lagrangian containing linear, quadratic (Gauss-Bonnet) and quartic terms for a ten-dimensional cosmological model cannot be solved ... [more ▼]

The Einstein-like field equations obtained from the variation of a Lagrangian containing linear, quadratic (Gauss-Bonnet) and quartic terms for a ten-dimensional cosmological model cannot be solved analytically. However, we can reduce them to a system of dynamical equations for the “Hubble parameters”. The study of the mathematical properties of the fixed points of this system gives a qualitative picture of the behaviour of a class of possible solutions. The inclusion of a quartic term generates an extremely rich structure for the corresponding dynamical system. Some solutions are shown to exhibit the interesting property of dimensional reduction, which has been proposed as a possible explanation of the three-dimensional character of our universe. [less ▲]

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See detailNeuroendocrine Mechanism of Onset of Puberty. Sequential Reduction in Activity of Inhibitory and Facilitatory N-Methyl-D-Aspartate Receptors
Bourguignon, Jean-Pierre ULg; Gerard, Arlette ULg; Alvarez Gonzalez, Maria-Luz ULg et al

in Journal of Clinical Investigation (1992), 90(5), 1736-44

In humans and in several animal species, puberty results from changes in pulsatile gonadotropin-releasing hormone (GnRH) secretion in the hypothalamus. In particular, the frequency of pulsatile GnRH ... [more ▼]

In humans and in several animal species, puberty results from changes in pulsatile gonadotropin-releasing hormone (GnRH) secretion in the hypothalamus. In particular, the frequency of pulsatile GnRH secretion increases at the onset of puberty, as can be shown by using hypothalamic explants of male rats of 15 and 25 d. Previous observations from us and others suggested that the initiation of puberty could involve a facilitatory effect of excitatory amino acids mediated through N-methyl-D-aspartate (NMDA) receptors. We found that GnRH secretion could be activated through NMDA receptors only around the time of onset of puberty (25 d). The aim of this study was to clarify why this activation did not occur earlier (at 15 d) and could no longer be observed by the end of puberty (at 50 d). We studied GnRH secretion in the presence of MK-801, a noncompetitive antagonist of NMDA receptors or AP-5, a competitive antagonist. We showed that, in the hypothalamus of immature male rats (15 d), a highly potent inhibitory control of pulsatile GnRH secretion in vitro was mediated through NMDA receptors. These data were confirmed in vivo because administration of the antagonist MK-801 (0.001 mg/kg) to immature male rats resulted in early pubertal development. Onset of puberty (25 d) was characterized by the disappearance of that NMDA receptor-mediated inhibition, thus unmasking a facilitatory effect also mediated through NMDA receptors. During puberty, there was a reduction in activity of this facilitatory control which was no longer opposed by its inhibitory counterpart. We conclude that a sequential reduction in activity of inhibitory and facilitatory NMDA receptors provides a developmental basis for the neuroendocrine mechanism of onset of puberty. [less ▲]

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See detailIn Vitro and in Vivo Modulation of 5-Hydroxytryptamine-, Thyrotropin-Releasing Hormone- and Calcitonin-Gene Related Peptide-Like Immunoreactivities in Adult Rat Sensory Neurons
Delree, P.; Martin, Didier ULg; Sadzot-Delvaux, Catherine ULg et al

in Neuroscience (1992), 51(2), 401-10

In a previous work we have shown that culturing adult rat dorsal root ganglia neurons modifies their neurotransmitter phenotype in such a way that cultured neurons synthesize transmitters that are not ... [more ▼]

In a previous work we have shown that culturing adult rat dorsal root ganglia neurons modifies their neurotransmitter phenotype in such a way that cultured neurons synthesize transmitters that are not found in situ, while several other transmitters are expressed in a much higher percentage of neurons in culture than in situ [Schoenen J. et al. (1989) J. Neurosci. Res. 22, 473-487]. The aim of the present study was to investigate the origin and the nature of the relevant environmental signals that allow this plasticity to be expressed, focusing on three neurotransmitters: 5-hydroxytryptamine, thyrotropin-releasing hormone and calcitonin-gene related peptide. The main results can be summarized as follows: (1) culturing cells in fetal calf serum or on feeder layers of astrocytes, Schwann cells or fibroblasts partially inhibits the serotoninergic phenotype of dorsal root ganglia neurons; (2) in vivo disconnection of dorsal root ganglia from their spinal targets but not from their peripheral or supraspinal targets induces a significant increase of the percentage of 5-hydroxytryptamine- and thyrotropin-releasing hormone-positive neurons in disconnected ganglia; (3) growth factors such as ciliary neuronotrophic factor or basic fibroblast growth factor but not nerve growth factor repress 5-hydroxytryptamine and calcitonin gene-related peptide immunoreactivity in cultured sensory neurons. In conclusion, neurotransmitter gene expression of adult dorsal root ganglia neurons is controlled by complex influences. Our data suggest that thyrotropin-releasing hormone and 5-hydroxytryptamine gene expression are tonically repressed in vivo by factors originating from the spinal segmental level and that growth factors such as ciliary neurotrophic factor or basic fibroblast growth factor could be potential vectors of this repressing effect. [less ▲]

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See detailMolecular Biology of Bovine Herpesvirus Type 4
Thiry, Etienne ULg; Bublot, M.; Dubuisson, J. et al

in Veterinary Microbiology (1992), 33(1-4), 79-92

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous virus of cattle. Its genome is a 144 +/- 6 kb double-stranded DNA consisting of a unique central part (L-DNA) flanked at both ends by tandem repeats ... [more ▼]

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous virus of cattle. Its genome is a 144 +/- 6 kb double-stranded DNA consisting of a unique central part (L-DNA) flanked at both ends by tandem repeats called polyrepetitive DNA (prDNA or H-DNA). The overall arrangement of genes has been obtained by the analysis of homologies between short BHV-4 DNA sequences and corresponding genes of Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS). The gene expression is temporally regulated. Glycoprotein precursor p (gp10/gp17) is expressed as gamma 1 polypeptide. Glycoproteins gp1, gp8, gp11 and their precursors are gamma 2 proteins. The analysis of strain variations allows the definition of two types of strains, based on the DNA patterns: the Movar 33/63-like and the DN 599-like strains. Only the M40 strain, isolated in India, fails to fit this classification. The genomic variations have been compiled to build a dendrogram showing three levels of divergence between BHV-4 strains or isolates. The available molecular data indicate that the BHV-4 genome shares much similarity with the DNA of EBV and HVS, two representative members of the gammaherpesvirinae. BHV-4 may therefore be classified in the subfamily gammaherpesvirinae. [less ▲]

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See detailMinimal Levels of Serum Estradiol Prevent Postmenopausal Bone Loss
Reginster, Jean-Yves ULg; Sarlet, Nathalie ULg; Deroisy, Rita ULg et al

in Calcified Tissue International (1992), 51

Biochemical parameters reflecting bone resorption [urinary calcium/creatinine (Ca/Cr) and hydroxyproline/creatinine (OH/Cr)] were related to serum estrogens [estrone (E1) and estradiol (E2)] in 262 ... [more ▼]

Biochemical parameters reflecting bone resorption [urinary calcium/creatinine (Ca/Cr) and hydroxyproline/creatinine (OH/Cr)] were related to serum estrogens [estrone (E1) and estradiol (E2)] in 262 healthy women including 158 patients receiving estrogen replacement therapy (ERT) for at least 6 months, 49 eugonadal women, and 55 untreated postmenopausal women. A significant (P < 0.001) correlation exists between serum E2 and Ca/Cr: Ca/Cr (mg/dl) = -0.00044 E2 (pg/ml) + 0.129 (n = 262; r = -0.37), serum E2 and OH/Cr: (OH/Cr (mg/g) = -0.049 E2 (pg/ml) + 18.76 (n = 262; r = -0.36), serum E1 and Ca/Cr: Ca/Cr (mg/dl) = -0.0003 E1 (pg/ml) + 0.127 (n = 261; r = -0.28) but not between serum E1 and OH/Cr. Women with circulating levels of E2 between 60 and 90 pg/ml have a significant (P < 0.01) reduction of Ca/Cr and OH/Cr when compared with those with lower levels of E2. Higher values of E2 do not provide additional benefit. We conclude that in postmenopausal women receiving an estrogen replacement therapy (ERT), a significant reduction of bone resorption is achieved when circulating levels of estradiol reach a value (60 pg/ml) corresponding to the one measured, in eugonadal women, during the last days of the early follicular phase of the menstrual cycle. We suggest that oral or percutaneous ERT should induce a minimal value of 60 pg/ml to prevent postmenopausal bone loss. [less ▲]

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See detailA Molecular Biologic Study of Extracellular Matrix Components During the Development of Glomerulosclerosis in Murine Chronic Graft-Versus-Host Disease
Munaut, Carine ULg; Bergijk, E. C.; Baelde, J. J. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1992), 67(5), 580-7

BACKGROUND: We studied the development of glomerulosclerosis in murine chronic graft-versus-host disease, a model for human systemic lupus erythematosus. EXPERIMENTAL DESIGN: The disease was induced in ... [more ▼]

BACKGROUND: We studied the development of glomerulosclerosis in murine chronic graft-versus-host disease, a model for human systemic lupus erythematosus. EXPERIMENTAL DESIGN: The disease was induced in (C57BL10 x DBA/2)F1 hybrids by injection of DBA/2 lymphocytes leading to deposition of auto-antibodies in the glomeruli, and a lupus type of nephritis morphologically. We have determined the levels of mRNA coding for laminin (B1 and B2), a 67 kilodalton laminin binding protein, and types I and IV collagen, in control and graft-versus host disease mice at various times after disease induction. RESULTS: Laminin and collagen mRNAs were increased in whole kidneys 4 weeks after induction of the disease. At week 10, all animals displayed dramatic stimulation of alpha 1(I), alpha 1(IV), laminin B1, and B2 mRNAs. The 67 kilodalton laminin binding protein mRNA was also doubled from week 4 to 16. In isolated glomeruli, the mRNA level coding for laminin B2 was already significantly increased from week 8. This enhancement of laminin synthesis corresponds to the mesangial expansion and to the development of laminin-containing spike formations of the glomerular basement membrane at week 8. CONCLUSIONS: The expansion of the mesangial matrix in murine chronic graft-versus-host disease is caused at least in part, by an increased production of extracellular matrix components by glomerular cells. These results demonstrate that the increase of specific extracellular matrix components mRNAs precedes light microscopic changes. Quantitative evaluation of the mRNA levels coding for extracellular matrix proteins may reveal a useful method for the early detection of the development of glomerular sclerosis at the stage preceding the onset of anatomo-clinical changes. [less ▲]

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See detailAdaptation du système cardio-respiratoire de la personne âgée soumise à l'entrainement physique
Hoffer, E.; Pierard, Luc ULg; Kulbertus, Henri ULg

in Revue Médicale de Liège (1992), 47(11), 560-72

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See detailLes syndromes hémorragiques pulmonaires diffus: approches clinique et diagnostique
Bury, Thierry ULg; Corhay, Jean-Louis ULg; Kotolenko, S. et al

in Revue Médicale de Liège (1992), 47(11), 554-9

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See detailElectro-optic coefficient measurements : correction of electric-field inhomogeneities in the transverse configuration
Lemaire, Philippe; Georges, Marc ULg

in Optics Letters (1992), 17(20), 1411-1413

The problem of electric-field inhomogeneities in the measurement of the electro-optic coefficient in the transverse configuration is well known. We present a simple way to solve these difficulties. It ... [more ▼]

The problem of electric-field inhomogeneities in the measurement of the electro-optic coefficient in the transverse configuration is well known. We present a simple way to solve these difficulties. It involves local measurements at several points along the transverse direction of the phase difference between two components of light traveling through the crystal. The electro-optic coefficient is simply calculated by integrating the phase shifts along the transverse profile. For this procedure one needs nothing but the value of the voltage applied to the crystal, and knowledge of the internal electric field is not necessary. Limits of application are discussed, and results are shown for photorefractive sillenite crystals. [less ▲]

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See detailTumor Cell Surface-Associated Binding Site for the M(R) 72,000 Type Iv Collagenase
Emonard, H. P.; Remacle, A. G.; Noël, Agnès ULg et al

in Cancer Research (1992), 52(20), 5845-8

We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M(r) 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By ... [more ▼]

We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M(r) 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By indirect immunofluorescence, staining with a specific anti-M(r) 72,000 type IV collagenase antibody was strongly induced when cells were preincubated with the purified enzyme. Scatchard plot analysis indicated the existence of a binding site for the M(r) 72,000 type IV collagenase with high affinity for both cell lines (Kd = 2 x 10(-9) M). These results are the first demonstration of the existence of a tumor cell membrane-associated putative receptor for a member of the matrix metalloproteinase family, as previously evidenced for the urokinase-type plasminogen activator. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 8. Synthesis and characterization of (meth)acrylic end-functionalized polymers: macromonomers and telechelics
Varshney, Sunil K; Bayard, Philippe; Jacobs, Christian et al

in Macromolecules (1992), 25(21), 5578-5584

Tailor-made poly(tert-butyl acrylate) macromonomers, which are useful building blocks for the synthesis of comb or multiblock copolymers, have been successfully synthesized by deactivation of living PtBA ... [more ▼]

Tailor-made poly(tert-butyl acrylate) macromonomers, which are useful building blocks for the synthesis of comb or multiblock copolymers, have been successfully synthesized by deactivation of living PtBA macroanions with suitable electrophiles bearing either styrene or methacrylic unsaturation. Mono- or bifunctional initiators such as sec-butyllithium or naphthalenyllithium can be used. When these initiators were modified by LiCl as a mu-type ligand, the macroanions which are known to be living were functionalized with high efficiency. Characterization of the various macromonomers was performed using H-1 NMR spectroscopy and SEC. [less ▲]

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See detailThe thermospheric odd nitrogen photochemistry - Role of non thermal N(4S) atoms
Shematovich, V. I.; Bisikalo, D. V.; Gérard, Jean-Claude ULg

in Annales Geophysicae [= ANGEO] (1992), 10

A nonequilibrium model to calculate the Maxwellian 'hot' N(4S) atom population using a Monte Carlo method is developed, and the NO vertical distribution for minimum solar cycle conditions is calculated ... [more ▼]

A nonequilibrium model to calculate the Maxwellian 'hot' N(4S) atom population using a Monte Carlo method is developed, and the NO vertical distribution for minimum solar cycle conditions is calculated. It is shown that the energetic photoelectrons produced by solar soft X-rays produce translationally 'hot' N(4S) atoms near 110 km which contribute significantly to the formation of the E-region NO peak observed at this altitude. Consideration of this new source of nitric oxide reconciles the need for an effective N(2D) quantum yield from N2 dissociation exceeding 50 percent derived from odd nitrogen models with the limits put on this value by laboratory and theoretical constraints. The altitude of the modeled NO peak is found to critically depend on the adopted N(2D) yield, and good agreement with the observed peak altitude and density is obtained using the quantum yield derived from theoretical considerations. [less ▲]

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See detailStructure, function, and fate of the BlaR signal transducer involved in induction of β-lactamase in Bacillus licheniformis
Zhu, Yingfang; Englebert, Serge; Joris, Bernard ULg et al

in Journal of Bacteriology (1992), 174(19), 6171-6178

The membrane-spanning protein BlaR is essential for the induction of beta-lactamase in Bacillus licheniformis. Its nature and location were confirmed by the use of an antiserum specific for its carboxy ... [more ▼]

The membrane-spanning protein BlaR is essential for the induction of beta-lactamase in Bacillus licheniformis. Its nature and location were confirmed by the use of an antiserum specific for its carboxy-terminal penicillin sensor, its function was studied by genetic dissection, and the structure of the penicillin sensor was derived from hydrophobic cluster analysis of the amino acid sequence by using, as a reference, the class A beta-lactamases with known three-dimensional structures. During the first 2 h after the addition of the beta-lactam inducer, full-size BlaR, bound to the plasma membrane, is produced, and then beta-lactamase is produced. By 2 h after induction, BlaR is present in various (membrane-bound and cytosolic) forms, and there is a gradual decrease in beta-lactamase production. The penicillin sensors of BlaR and the class D beta-lactamases show strong similarities in primary structures. They appear to have the same basic spatial disposition of secondary structures as that of the class A beta-lactamases, except that they lack several alpha helices and, therefore, have a partially uncovered five-stranded beta sheet and a more readily accessible active site. Alterations of BlaR affecting conserved secondary structures of the penicillin sensor and specific sites of the transducer annihilate beta-lactamase inducibility. [less ▲]

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See detailThe Beta Pictoris circumstellar disk. XIII - Survey of the variable CA II lines
Lagrange-Henri, A. M.; Gosset, Eric ULg; Beust, H. et al

in Astronomy and Astrophysics (1992), 264

Over 200 Ca II K and H spectra of Beta Pictoris taken between September 19, 1989 and December 19, 1990 are presented. Many new variable features are observed, at low and high redshifts, together with a ... [more ▼]

Over 200 Ca II K and H spectra of Beta Pictoris taken between September 19, 1989 and December 19, 1990 are presented. Many new variable features are observed, at low and high redshifts, together with a few blueshifted ones. The time scales for the variations are shown to be strongly dependent on the redshift of the variable lines. A rate of events of about 200/yr is derived; it is also shown that in some cases the absorbing cloud covers less than 40 percent of the stellar surface. The observed variations are analyzed and compared to those observed in the UV. These observations make it possible to test the present scenario of evaporating infalling bodies, in terms of velocity of variable lines, occurrence of variable events, small time-scale variations, and size of the absorbing cloud. It is shown that the observed velocity range for the variable components may be explained by the present scenario, as well as the size of the absorbing clouds. [less ▲]

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See detailPhotometric accuracy - The impact of extrapolation in differing color-transformation schemes
Manfroid, Jean ULg; Sterken, C.; Gosset, Eric ULg

in Astronomy and Astrophysics (1992), 264

We discuss photometric errors that arise from the use of differing color-transformation schemes when extrapolating outside the range of the standard values that define the system. An example based on data ... [more ▼]

We discuss photometric errors that arise from the use of differing color-transformation schemes when extrapolating outside the range of the standard values that define the system. An example based on data from the Long-Term Photometry of Variables project (LTPV) at ESO is analyzed. Practically, the extrapolation errors are most evident as systematic brightness jumps of some program stars, seen when monitored over several observing runs. Conclusions are drawn about the choice of standard stars in the Stromgren system. [less ▲]

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See detailThe influence of blood transfusion on brain blood flow autoregulation among stable preterm infants.
RAMAEKERS, Vincent ULg; Casaer, P.; Daniels, H. et al

in Early human development (1992), 30(3), 211-20

Prior to and 24 h following blood transfusion serial determinations of both cerebral artery flow velocity waveforms and mean arterial blood pressure have been used to reconstruct the autoregulatory curve ... [more ▼]

Prior to and 24 h following blood transfusion serial determinations of both cerebral artery flow velocity waveforms and mean arterial blood pressure have been used to reconstruct the autoregulatory curve and its upper blood pressure limit among five stable preterm infants. Prior to transfusion the autoregulatory range of cerebral blood flow (CBF) was narrow due to a relatively low-set upper blood pressure limit. At 24 h after transfusion each individual has been re-examined. Following correction of anemia both a significant reduction of CBF velocities as well as a concomitant rise of the Pulsatility Index (PI) occurred over the entire range of blood pressures indicating a reduction of CBF after transfusion. In addition a right-sided shift of the upper limit towards higher mean blood pressures occurred after transfusion and resulted in an extension of the autoregulatory plateau of CBF. These favourable effects of blood transfusion ameliorating autoregulation of brain blood flow particularly at higher blood pressures might well bear important therapeutic perspectives in our effort to prevent intracranial haemorrhage among sick preterm infants. [less ▲]

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