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See detailParadoxical Sleep Deprivation Increases the Content of Glutamate and Glutamine in Rat Cerebral Cortex
Bettendorff, Lucien ULg; Sallanon-Moulin, M.; Touret, Monique et al

in Sleep (1996), 19(1), 65-71

We investigated the influence of the sleep/waking cycle, the effects of paradoxical sleep deprivation (PSD) and of the vigilance-promoting drug modafinil on the amino acid contents of rat brain cortex. No ... [more ▼]

We investigated the influence of the sleep/waking cycle, the effects of paradoxical sleep deprivation (PSD) and of the vigilance-promoting drug modafinil on the amino acid contents of rat brain cortex. No significant nycthemeral variations in amino acid levels could be detected. PSD (12-24 hours), using the water tank method, significantly increased the levels of glutamate and glutamine. The increase was still observed after the sleep rebound period. gamma-Aminobutyric acid (GABA) levels did not change significantly during the instrumental sleep deprivation but increased during the rebound period. Control experiments indicate that the increase in glutamate and glutamine levels is due to PSD rather than to the stress associated with the experimental procedure. The increase in glutamate content cannot arise only from transamination reactions, because the levels of other amino acids (such as aspartate) did not decrease. Modafinil treatment did not significantly modify the brain cortex content of any of the amino acids tested. [less ▲]

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See detailVérification microbiologiques et sérologiques des donneurs de cornées
Weekers, J. F.; Melin, Pierrette ULg; Lejeune, Tony ULg et al

in Bulletin de la Société Belge d'Ophtalmologie (1996), 260

A study was made during 1994 about microbiological contamination on the donor eyes before enucleation of whole globe. We studied the efficiency of the decontamination method by checking sterility of ... [more ▼]

A study was made during 1994 about microbiological contamination on the donor eyes before enucleation of whole globe. We studied the efficiency of the decontamination method by checking sterility of corneal storage medium after two days. We give results of serological tests on 252 donors. [less ▲]

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See detailCellular and molecular mechanisms involved in thymic T-cell education to neuroendocrine self principles
Geenen, Vincent ULg; Benhida, Abdellah; Kecha, Ouafae et al

in International Journal of Thymology (1996), 4

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See detailDéveloppement d'un modèle de type arbre appliqué à la croissance des peuplements feuillus irréguliers du sud de la Belgique
Lejeune, Philippe ULg

in Canadian Journal of Forest Research = Journal Canadien de la Recherche Forestière (1996), 26(10), 1838-1848

A model predicting circumference growth of individual trees has been developed for mixed irregular stands dominated by Fagus silvatica L. in southeastern Belgium. This model integrates simultaneously tree ... [more ▼]

A model predicting circumference growth of individual trees has been developed for mixed irregular stands dominated by Fagus silvatica L. in southeastern Belgium. This model integrates simultaneously tree, stand, and growing site characteristics without considering distances between individual trees. It has a determination coefficent of 40.1 % and a residual standard deviation of 0.45cm/year. Forest mensuration variables considered in the model are circumference, tree social position represented by the total basal area of trees greater than the subject tree, stand basal area, and some index of stand structure corresponding to the ratio of the variance over the mean circumference. The site component is essentially expressed by the length of the growing period. The low accuracy obtained for individual tree growth is relative because the results are used mainly after individual trees have been distributed into size classes. We have been able to show that the introduction of crown description in the model can increase its accuracy. But this modification requires a submodel describing crown changes over time. [less ▲]

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See detailCloning and Sequencing of the Dnak Locus in Streptomyces Coelicolor A3(2)
Brans, Alain ULg; Loriaux, Axelle; Joris, Bernard ULg et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1996), 6(3), 179-84

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK ... [more ▼]

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively. [less ▲]

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See detailThe Erythrocyte/Brain Glucose Transporter (Glut1) May Adopt A Two-Channel Transmembrane Alpha/Beta Structure.
Ducarme, P.; Rahman, M.; Lins, Laurence ULg et al

in Journal of Molecular Modeling (1996), 2(2), 27-45

There are two models of topology for the membrane domains of the erythrocyte/brain facilitative glucose transporter, GLUT1. The first is composed of 12 membrane-spanning a-helices, the second of 16 ... [more ▼]

There are two models of topology for the membrane domains of the erythrocyte/brain facilitative glucose transporter, GLUT1. The first is composed of 12 membrane-spanning a-helices, the second of 16 membrane-spanning b-strands. We have used Jähnig’s and Eisenberg’s methods to identify possible transmembrane segments (10 spanning a-helices and 4 b-strands). The topology proposed is more consistent with available experimental data from FTIR, CD and mapping experiment than the previous models . We suggest that GLUT1 might form two channels, one of which is responsible for glucose transport. This agrees with the theoretical and experimental arguments. Finally, an analysis of the mutation periodicity and of the mean hydrophobicity for the GLUT family is provided in order to evaluate the packing of the protein in the membrane. Keywords: [less ▲]

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See detailSensitivity calculations and variance analysis in plant measurement reconciliation
Heyen, Georges ULg; Marechal, Eric; Kalitventzeff, Boris ULg

in Computers & Chemical Engineering (1996), 20(Suppl. A), 539-544

Analysis of the results of a data reconciliation program is made easier by extracting more information from the Jacobian matrix of the constraint equations. Standard deviation for all state variables ... [more ▼]

Analysis of the results of a data reconciliation program is made easier by extracting more information from the Jacobian matrix of the constraint equations. Standard deviation for all state variables (measured or not measured) is related to the standard deviation of measurements. Distinction between variables that are actually corrected by the validation process, and those that are merely derived from a single measurement is straightforward. Based on this information, decisions can be taken : deletion of unnecessary measurements, addition of new measurement points and their optimal selection, or identification of key measurements for which any enhancement of accuracy would result in significant improvement in the quality of the process validation. [less ▲]

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See detailCost effectiveness of treatment in osteoporosis
Reginster, Jean-Yves ULg

in Journal of Rheumatology (1996), 23(7), 1312-1313

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See detailNouveaux bisphosphonates dans l’ostéoporose : craintes et espoirs
Reginster, Jean-Yves ULg

in Revue du Rhumatisme (1996), 63(7-8), 545-548

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See detailLes xenika et les barbarika onomata dans les théories linguistiques gréco-latines
Rochette, Bruno ULg

in Antiquité Classique : Revue Interuniversitaire d'Etudes Classiques (1996), 65

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See detailTheory of laser-induced two-step electron-ion recombination
Schlagheck, Peter ULg; Maragakis, P.; Lambropoulos, P.

in Zeitschrift für Physik. D, Atoms, Molecules, and Clusters (1996), 37(1), 19-27

A theoretical framework for the quantitative interpretation of laser-induced electron-ion recombination is presented in terms of the density matrix taking into account stochastic bandwidth and pulse ... [more ▼]

A theoretical framework for the quantitative interpretation of laser-induced electron-ion recombination is presented in terms of the density matrix taking into account stochastic bandwidth and pulse duration of the laser. The theory deals with recombination induced from the continuum into one group of states by one laser and further stabilized into a lower group of states by a second laser. Rate equations are then derived from the complete density matrix, and the conditions for their validity are discussed. Both density matrix and rate equations are applied to the interpretation of a recent experiment with equally good agreement. [less ▲]

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See detail"Born to be wild oder doch geleernt?" Neue verhaltensmedizinische Erkenntnisse zur Ätiopathogenese der Migräne
Gerber, W. D.; Kropp, P.; Schoenen, Jean ULg et al

in Verhaltenstherapie (1996), 6

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See detailMuscle protein metabolism in relation to growth rate in double muscled Belgian Blue bulls: an integrated approach
Van Eenaeme, Christian ULg; Hornick, Jean-Luc ULg; Baldwin, Paule et al

in Mededelingen van de Faculteit van Landbouwwetenschappen, Rijksuniversiteit, Gent (1996), 61/4a

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See detailBronchopulmonary and Disseminated Granulomatous Disease Associated with Aspergillus Fumigatus and Candida Species Infection in a Golden Retriever
Clercx, Cécile ULg; Mc Entee, Kathleen ULg; Snaps, Frédéric ULg et al

in Journal of the American Animal Hospital Association (1996), 32(2, Mar-Apr), 139-45

A seven-year-old, female golden retriever was referred for a paroxysmal, chronic cough and dyspnea, dysphagia, facial pruritus, anterior uveitis, and deteriorating general condition. A severe, mixed ... [more ▼]

A seven-year-old, female golden retriever was referred for a paroxysmal, chronic cough and dyspnea, dysphagia, facial pruritus, anterior uveitis, and deteriorating general condition. A severe, mixed interstitial and alveolar pattern, with poorly defined amorphous lesions, was seen on thoracic radiographs. Multiple, whitish nodules disseminated on the hyperemic respiratory mucosa were noted on bronchoscopy. Escherichia coli and Aspergillus fumigatus were cultured from the bronchoalveolar lavage. Granulomatous lesions in numerous organs were identified during necropsy, and Aspergillus fumigatus and Candida spp. were cultured from lung and kidney tissues. Microscopic granulomatous lesions were compatible with mycotic infection; however fungal organisms were not observed. [less ▲]

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See detailCardiovascular risk factors in children from the Belgian province of Luxembourg. The Belgian Luxembourg Child Study.
Guillaume, Michèle ULg; Lapidus, L.; Beckers, L. et al

in American Journal of Epidemiology (1996), 144(9), 867-80

The Province of Luxembourg is an area in Belgium with a high prevalence of risk factors for cardiovascular disease and non-insulin-dependent diabetes mellitus among adults. In the present study, children ... [more ▼]

The Province of Luxembourg is an area in Belgium with a high prevalence of risk factors for cardiovascular disease and non-insulin-dependent diabetes mellitus among adults. In the present study, children in the age groups 6-8, 8-10, and 10-12 years were selected at random from school classes (n = 1,028), with a participation rate of 70.3%. Anthropometric factors, blood pressure, and fasting blood glucose, plasma cholesterol, triglyceride, and insulin levels were measured in 1992. All anthropometric and metabolic variables increased with age, except for waist: hip circumference ratio in boys and cholesterol in girls. In the oldest group, girls who had passed menarche were taller and heavier and had greater skinfold, body mass index, insulin, and systolic blood pressure values but lower total cholesterol levels and waist: hip ratios than girls who had not passed menarche. Boys had lower skinfolds and higher waist: hip ratios than girls in all age groups, and were significantly shorter and lighter in the oldest age group. There was no difference in body mass index between the two sexes. Girls had higher triglyceride and insulin levels in the 10- to 12-year age group, lower blood glucose values in the 8-10 and 10-12 age groups, and lower diastolic blood pressures in the 8-10 age group. Obesity, blood glucose, triglycerides, insulin, and blood pressure were highly interrelated. Cholesterol, triglycerides, insulin, and blood pressure values were all among the highest of values previously reported in other studies. The deciles of body mass index above 50 appeared to be particularly elevated, suggesting that obesity, when present, was pronounced in this population of children. These findings suggest an accumulation of genetic susceptibility to cardiovascular disease and non-insulin-dependent diabetes mellitus in this stable, ethnically homogeneous, and rather isolated part of continental Europe. [less ▲]

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See detailIn vitro activity of amoxycillin/clavulanate and ticarcillin/clavulanate compared with that of other antibiotics against anaerobic bacteria: comparison with the results of the 1987 survey
Pierard, denis; De Meyer A; Rosseel, P. et al

in Acta Clinica Belgica (1996), 51(2), 70-9

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight ... [more ▼]

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight Belgian university hospitals and compared with that of 8 other antibiotics using the NCCLS reference agar dilution procedure. Production of beta-lactamase was detected by the nitrocefin test in 48% of the isolates. At NCCLS-recommended breakpoints, more than 90% of isolates were susceptible to amoxycillin/clavulanate, ticarcillin/clavulanate, piperacillin/tazobactam, imipenem, chloramphenicol and metronidazole but only 77%, 72% and 48% to cefoxitin, clindamycin and penicillin, respectively. In comparison with the results of a similar survey conducted in 1987 no major changes in susceptibility were observed except for the susceptibility to clindamycin that declined from 83% to 72% overall, and from 83% to 66% in the B. fragilis group. Furthermore one isolate of Clostridium clostridioforme was found produce beta-lactamase and few B. fragilis group isolates showed reduced susceptibility to metronidazole. [less ▲]

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See detailComparaison des effets de différents types de traitement phytosanitaires des semences de cotonnier au Burundi et en Grèce
Carême, C.; Mergeai, Guy ULg; Ydraiou, Francesca et al

in Tropicultura (1996), 14

Les effets de plusieurs matières actives insecticides et de différentes techniques de traitement des semences du cotonnier ont été étudiés lors de 7 essais réalisés au Burundi et en Grèce en 1993 et 1994 ... [more ▼]

Les effets de plusieurs matières actives insecticides et de différentes techniques de traitement des semences du cotonnier ont été étudiés lors de 7 essais réalisés au Burundi et en Grèce en 1993 et 1994. Les essais menés au Burundi mettent en évidence l'efficacité très hautement significative sur Aphis gossypii Glover de l'imidaclopride à la dose de 3g m.a./kg, associé au chlorothalonil à 0,8 g/kg semences, utilisé en enrobage des semences délintées. L'absence de pression parasitaire n'a pas permis de départager les traitements testés en Grèce en ce qui concerne leur efficacité pour lutter contre les ravageurs qui attaquent le cotonnier au début de son cycle. Les observations écophysiologiques réalisées au cours de ces expérimentations mettent en évidence une amélioration significative de la précocité de la maturation des capsules chez les plantes issues de graines enrobées portant une dose moyenne de 2,2 g d'imidaclopride et 2,4 g de TMTO par kg de semences. Le gain de précocité est en moyenne de 10 jours par rapport au traitement témoin. [less ▲]

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See detailLichens et champignons lichénicoles nouveaux ou intéressants pour la flore de Belgique et des régions voisines. VII.
van den Boom, Pieter; Diederich, Paul; Sérusiaux, Emmanuël ULg

in Bulletin de la Société des Naturalistes Luxembourgeois (1996), 97

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See detailMorphometric, immunohistological and three-dimensional evaluation of the endometrium of menopausal women treated by oestrogen and Crinone, a new slow-release vaginal progesterone.
Casanas-Roux, Françoise; NISOLLE, Michelle ULg; Marbaix, Etienne et al

in Human Reproduction (1996), 11(2), 357-63

Recently advanced computerized technology was applied to the investigation of morphometric, immunohistological and three-dimensional changes of the endometrial mucosa in order to evaluate quantitatively ... [more ▼]

Recently advanced computerized technology was applied to the investigation of morphometric, immunohistological and three-dimensional changes of the endometrial mucosa in order to evaluate quantitatively the effects of three doses of a new slow-release vaginal progesterone on the endometrium in post-menopausal women. A total of 20 menopausal women, deprived of ovarian function, were given oestrogen for 12 days and a combined therapy of oestrogen (administered orally) and progesterone for another 12 day period. Progesterone was administered vaginally through a new gel (Crinone) utilizing a bioadhesive, biocompatible polymer as a base to achieve a sustained release effect. An endometrial biopsy was taken before treatment, after oestrogen-only treatment and after the oestro-progestogen therapy. Before treatment, all the patients exhibited an atrophic endometrium. After oestrogen-only treatment, typical proliferative changes occurred: an increase in the endometrium thickness, an increase in the mitotic index, numerous cylinder-like glands and no coiled glands, and high concentrations of oestrogen receptors (ER) and progesterone receptors (PR). After the oestro-progestogen therapy, whatever the dose of progesterone given, a secretory transformation of the endometrial mucosa occurred, mitotic activity decreased significantly, more ramified and coiled glands were observed, and a decrease in PR content was noted in epithelial and stromal nuclei, and a decrease in PR content was also observed in epithelial nuclei but not in stromal nuclei. Accurate new techniques of image analysis have shown that crinone therapy could eliminate the proliferative effects of oestrogen treatment in post-menopausal women, despite doses as low as 45 mg of progesterone administered vaginally every other day. The results suggest that the sustained release effects of Crinone are clinically relevant. [less ▲]

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See detailLa nutrition et le couple "mère-enfant"
Hennart, P; Porignon, Denis ULg; Donnen, P

in Académie Royale des Sciences d'Outre-Mer (1996)

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See detailThe existence of an urban underclass in Belgium
Martiniello, Marco ULg

in New Community (1996), 22(4), 655-669

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See detailThe human 37kDa laminin receptor precursor/p40 ribosomal associated protein multigene family : structure of the active gene and chromosomal location at 3p21.3
Jackers, Pascale ULg; Minoletti, Fabiola; Belotti, Dorina et al

in Cell Biology International (1996), 20

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See detailLa traversée des candiatures universitaires : Une analyse de cas
Beckers, Jacqueline ULg; Boxus, Elise ULg

in Res Academica (1996), 13(1-2), 85-104

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See detailGrowth hormone response to apomorphine in obsessive-compulsive disorder.
Pitchot, William ULg; Hansenne, Michel ULg; Moreno, A. G. et al

in Journal of Psychiatry & Neuroscience [=JPN] (1996), 21(5), 343-5

Several lines of evidence suggest that dopamine plays a role in the pathophysiology of obsessive-compulsive disorder (OCD). Indeed, some trials have shown the efficacy of neuroleptic addition in the ... [more ▼]

Several lines of evidence suggest that dopamine plays a role in the pathophysiology of obsessive-compulsive disorder (OCD). Indeed, some trials have shown the efficacy of neuroleptic addition in the treatment of OCD patients. In this study, we assessed the growth hormone (GH) response to 0.5 mg apomorphine(sc) in 8 drug-free inpatients (6 male, 2 female; mean age +/- SD = 34.7 +/- 12.6) meeting DSM-III-R criteria for OCD without major depression and compared their responses with those of 8 healthy male volunteers (mean age = 27.1 +/- 8.5). The groups did not differ in their mean GH peak response: 12.4 +/- 9.7 ng/mL in OCD patients versus 21.1 +/- 14.2 ng/mL in normal controls (F = 0.9, df1, 14, P = 0.37). These results do not support the hypothesis of dopaminergic overactivity in OCD. In fact, the completely blunted GH response to apomorphine in 2 OCD patients suggests the biological heterogeneity of OCD. Some dopaminergic disturbances could be observed in patients with comorbid diagnoses or patients unresponsive to serotonin reuptake inhibitors, but the results of this study require confirmation from a larger sample with a precise assessment of comorbidity. [less ▲]

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See detailLa Biologie clinique en pédiatrie: hypotonie et retard moteur
Misson, Jean-Paul ULg; Leroy, Patricia; Dubru, Jean-Marie ULg et al

in Acta Paediatrica Belgica (1996), 28(2), 95

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See detailThree hTIM mutants that provide new insights on why TIM is a dimer
Mainfroid, Véronique; Terpstra, Peter; Beauregard, Marc et al

in Journal of Molecular Biology (1996), 257(2), 441-56

Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced, in ... [more ▼]

Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced, in which a glutamine residue was substituted for either Met14 or Arg98, both of which are interface residuces. These substitutions strongly interfere with TIM subunit association, since these mutant TIMs appear to exist as compact monomers in dynamic equilibrium with dimers. In kinetic studies, the M14Q mutant exhibits significant catalytic activity, while the R98Q enzyme is inactive. The M14Q enzyme is nevertheless much less active than unmutated hTIM. Moreover, its specific activity is concentration dependent, suggesting a dissociation process in which the monomers are inactive. In order to determine the conformational stability of the wild-type and mutant hTIMs, unfolding of all three enzymes was monitored by circular dichroism and tryptophan fluorescence spectroscopy. In each case, protein stability is concentration dependent, and the unfolding reaction is compatible with a two-state model involving the native dimer and unfolded monomers. The conformational stability of hTIM, as estimated according to this model, is 19.3 (+/-0.4) kcal/mol. The M14Q and R98Q replacements significantly reduce enzyme stability, since the free energies of unfolding are 13.8 and 13.5 (+/- 0.3) kcal/mol respectively, for the mutants, A third mutant, in which the M14Q and R98Q replacements are cumulated, behaves like a monomer. The stability of this mutant is not concentration-dependent, and the unfolding reaction is assigned to a transition from a folded monomer to an unfolded monomer. The conformational stability of this double mutant is estimated 2.5 (+/-0.1) kcal/mol. All these data combined suggest that TIM monomers are thermodynamically unstable. This might explain why TIM occurs only as a dimer. [less ▲]

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See detailDu gang au groupe social: une analyse socio-préventive
Comeron, Manuel ULg

in Cahiers de la Sécurité (1996)

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de ... [more ▼]

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de la recherche qui évalue aussi les politiques publiques de prévention. [less ▲]

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See detailUn nouveau département de français à l'Université de Liège : ses précédents, ses missions, ses projets, son équipe
Defays, Jean-Marc ULg

in Français 2000 : Le point sur le Français langue étrangère (1996), (150-151), 60-62

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See detailPrediction of signal peptide functional properties: a study of the orientation and angle of insertion of yeast invertase mutants and human apolipoprotein B signal peptide variants.
Talmud, P.; Lins, Laurence ULg; Brasseur, Robert ULg

in Protein Engineering (1996), 9(4), 317-21

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using ... [more ▼]

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using molecular modelling of the invertase signal peptide we have analysed the hydrophobicity potential and the change in dielectric constant of the energy transfer, when the molecule moves from a hydrophobic to a hydrophilic phase at the simulated hydrophobic-hydrophilic interface. This modelling has been carried out on wild type and mutant invertase signal peptides of altered function, previously reported in the literature. While the predicted angle of insertion correlates with the measured extent of invertase secretion, with an optimum angle of 45 degrees, mutations that change the angle of orientation reduce the extent of invertase secretion. We have applied these same molecular modelling principles to the naturally occurring variants of the human apolipo-protein B (apoB) signal peptide, that confer a secretion defective phenotype when fused to yeast invertase and expressed in yeast. Our modelling thus identifies a strong correlation between the predicted angle of insertion of the signal peptide into the membrane and its ability to direct secretion. [less ▲]

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See detailLe chaponnage, une technique qui revient à la mode!
Marlier, Didier ULg; Vindevogel, Henri ULg

in Annales de Médecine Vétérinaire (1996), 140

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See detailType II to type I transformation of chronically stimulated goat latissimus dorsi muscle: a histoenzymological, biochemical, bioenergetic, and functional study.
Radermacker, M. A.; Focant, B.; Hautecler, T. et al

in European Surgical Research = Europäische Chirurgische Forschung = Recherches Chirurgicales Européennes (1996), 28

Five goat latissimus dorsi muscles (LDM) were submitted to a progressive chronic electrostimulation program to reach an integrated understanding of the fast-to-slow transformation process in large mammals ... [more ▼]

Five goat latissimus dorsi muscles (LDM) were submitted to a progressive chronic electrostimulation program to reach an integrated understanding of the fast-to-slow transformation process in large mammals. LDM were regularly sampled and followed during a period of 8 months. Each sample was simultaneously assessed for histoenzymological study, myosin and LDH isoforms and bioenergetic capacities [NADH dehydrogenase cytochrome c oxidoreductase (NADH Cyt c OR), succinate dehydrogenase cytochrome c oxidoreductase (Succ Cyt c OR), cytochrome c oxidase (Cyt c Ox) and LDH]. Such muscles were also tested with and without completion of II to I transformation for their mechanical properties in isometric and isotonic strain gauge testing. The conversion of fast-to-slow myosin monitored by heavy chain (HC I) and light chain slow component (LC2s) began a few days after stimulation and was almost 100% after 100 days. The H-LDH isoforms evolved similarly but did not reach 100% conversion after 200 days. The activity of respiratory chain oxidases increased within 36 h but to a variable extent and peaked after 32 days, corresponding to a 75% transformation of myosin compared to initial levels. NADH Cyt c OR, Succ Cyt c OR, and Cyt c Ox, respectively increased 10-, 5- and 5-fold. These activities then significantly decreased before the completion of the myofibrillar transformation and reached a plateau with stable activities that remained 2- to 3-fold higher than the unstimulated LDM. LDH activity sharply decreased until day 62 (5-fold) and then plateaued. Functionally, muscle showed a reduced speed of contraction and moderate reduction in power output but had become fatigue-resistant. This study documents the transformation process in large mammals and suggests the dynamic relation between workload, aerobic-anaerobic metabolism and the contractile myofibrillar system. [less ▲]

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See detailLa myxomatose amyxomateuse: isolement de trois souches en Belgique
Marlier, Didier ULg; Vindevogel, Henri ULg

in Annales de Médecine Vétérinaire (1996), 140

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See detailIdentification of antigens recognized in the developing mouse brain by the RC2 antibody, a marker of radial glia
Leprince, Pierre ULg; Chanas-Sacre, G.; Wattiez, R. et al

in International Journal of Developmental Neuroscience (1996), 14(Sup. 1), 84

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See detailComparison of food conversion by the Pirapatinga Piaractus brachypomus under different times of feeding
Baras, E.; Mélard, Charles ULg; Grignard, J. C. et al

in Progressive Fish-Culturist (1996), 58

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See detailLa reconnaissance des répudiations. Note sous Cass., 11 décembre 1995
Carlier, Jean-Yves ULg

in Revue Trimestrielle de Droit Familial [=RTDF] (1996)

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See detailLes maladies virales chez le lapin européen (Oryctolagus cuniculus)
Marlier, Didier ULg; Vindevogel, Henri ULg

in Annales de Médecine Vétérinaire (1996), 140

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See detailWhat about lorisk Figo Stage Ia and Ib, G1-G2 endometrial adenocarcinoma ?
Delaloye, J-F; Megalo; COUCKE, Philippe ULg et al

in Radiotherapy & Oncology (1996), 43(Supp 1), 6

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See detailEffect of total treatment time on event-free survival in carcinoma of the cervix
Delaloye, J-F; COUCKE, Philippe ULg; Pampallona, S et al

in Gynecologic Oncology (1996), 60

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See detailStructure-specific nuclease activity in yeast nucleotide excision repair protein RAD2
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1995), 270(50), 30194-8

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See detailDynamic properties of the transient network formed by telechelic ionomers studied by dynamic light scattering and dynamic mechanical analysis
Johannsson, Ragnar; Chassenieux, Christophe; Durand, Dominique et al

in Macromolecules (1995), 28(25), 8504-8510

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L ... [more ▼]

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L, which is well below the overlap concentration of the polymer chains (C*), a transient network is formed characterized by a plateau modulus at high frequencies and a relatively narrow viscoelastic relaxation time distribution. The intensity autocorrelation function of the transient network shows two relaxational modes: a relatively fast diffusional mode close to the co-operative diffusional mode of semidilute solutions of the unfunctionalized polymers and a slow broader mode which becomes independent of the scattering angle at a concentrations close to C*. The relation between this slow mode and the viscoelastic relaxation is discussed. Both the slow mode and the viscoelastic relaxation processes have an Arrhenius temperature dependence with the same activation energy, E(a) = 25 kJ/mol. [less ▲]

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See detailThe robustness of self-organized criticality against extinctions in a tree-like model of evolution
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Europhysics Letters [=EPL] (1995), 32(7), 613-618

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the ... [more ▼]

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the model leads to self-organized criticality. For r not equal 0, a transition from growing trees to finite ones takes place at some critical r(c) = 0.48 +/- 0.01 value. For 0 less than or equal to r less than or similar to r(c), self-organized criticality is thus robust against extinctions of the weakest species. The size distribution of avalanches follows a power law behaviour with an exponent 3/2 which seems to be independent of the parameter r. The growing trees are found to be self-similar with a non-universal fractal dimension D-f non-trivially ranging from 2 to 1 depending on the parameter r. This constraint model opens up the field of description of various possible physical events for such an evolution model. [less ▲]

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See detailEIT: Extreme-Ultraviolet Imaging Telescope for the SOHO Mission
Delaboudinière, J.-P.; Artzner, G. E.; Brunaud, J. et al

in Solar Physics (1995), 162

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer ... [more ▼]

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer-coated optics will select spectral emission lines from Fe IX (171 Å), Fe XII (195 Å), Fe XV (284 Å), and He II (304 Å) to provide sensitive temperature diagnostics in the range from 6 × 10[SUP]4[/SUP] K to 3 × 10[SUP]6[/SUP] K. The telescope has a 45 x 45 arcmin field of view and 2.6 arcsec pixels which will provide approximately 5-arcsec spatial resolution. The EIT will probe the coronal plasma on a global scale, as well as the underlying cooler and turbulent atmosphere, providing the basis for comparative analyses with observations from both the ground and other SOHO instruments. This paper presents details of the EIT instrumentation, its performance and operating modes. [less ▲]

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See detailPre- and Post-Translational Regulation of Aromatase by Steroidal and Non-Steroidal Aromatase Inhibitors
Foidart, Agnès ULg; Tlemcani, O.; Harada, N. et al

in Brain Research (1995), 701(1-2), 267-78

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir ... [more ▼]

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir) cells and the concentration of aromatase mRNA as measured by RT-PCR in the brain. All these effects can be mimicked by estrogens. The behavioral effects of T can be blocked by a variety of aromatase inhibitors and, in parallel, the AA is strongly inhibited in the preoptic area (POA). We showed recently that the steroidal inhibitor, 4-OH-androstenedione (OHA) markedly decreases the immunostaining density of brain ARO-ir cells while the non-steroidal inhibitor, R76713 (racemic Vorozole; VOR) unexpectedly increased the density of this staining, despite the fact that the enzyme activity was completely inhibited. To generalize these findings and try to identify the underlying mechanism, we compared here the effects of two steroidal (OHA and androstatrienedione [ATD]) and two non-steroidal (VOR and Fadrozole [FAD]) aromatase inhibitors on the aromatase immunostaining and aromatase mRNA concentration in the brain of castrated quail concurrently treated with T. The 4 inhibitors significantly blocked the activation by T of male copulation. The two steroidal inhibitors decreased the immunostaining of brain ARO-ir cells but both VOR and FAD markedly enhanced the density of this staining. In parallel, OHA and ATD completely blocked the T-induced increase in aromatase mRNA concentration, while VOR and FAD had no effect on these RNA concentrations in the POA-anterior hypothalamus and they decreased them only slightly in the posterior hypothalamus. Taken together these results suggest that the inhibition of AA by ATD or OHA and the subsequent removal of locally produced estrogens blocks the synthesis of aromatase presumably at the transcriptional level. By contrast, the two non-steroidal inhibitors tested here block AA but in parallel increase the aromatase immunostaining. This effect does not result from an enhanced transcription and it is therefore speculated that these compounds increase either the translation of the aromatase mRNA or the half-life of the protein itself. [less ▲]

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See detailLes clivages entérinés dans la constitution belge
Verjans, Pierre ULg

in Agenda Interculturel (1995), (139), 19-24

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See detailThe Beta-Lactamase Secreted by the Antarctic Psychrophile Psychrobacter Immobilis A8
Feller, Georges ULg; Sonnet, P.; Gerday, Charles ULg

in Applied and Environmental Microbiology (1995), 61(12), 4474-6

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal ... [more ▼]

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases. [less ▲]

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See detailThe use of styrenic copolymers to generate polyimide nanofoams
Hedrick, James; Hawker, Craig J; DiPietro, R. et al

in Polymer (1995), 36(25), 4855-4866

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block ... [more ▼]

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block copolymers comprising a thermally stable block and a thermally labile material. At properly designed volume fractions, the morphology provides a matrix of the thermally stable material with the thermally labile material as the dispersed phase. Upon thermal treatment, the thermally unstable block undergoes thermolysis generating pores, the size and shape of which are dictated by the initial copolymer morphology. Several labile blocks were surveyed including polystyrene, poly(alpha-methylstyrene) and several alpha-methylstyrene/styrene copolymers. Each of these polymers can unzip to its monomer upon heating; however, the rate is substantially slower for polystyrene. The copolymers were synthesized through either the poly(amic acid) precursor, followed by chemical imidization to the polyimide form, or the poly(amic alkyl ester) precursor followed by thermal imidization. The decomposition of the labile coblock was studied by thermogravimetric and dynamic mechanical analysis. Upon decomposition, the foams showed pore sizes in the nanometre regime along with the expected reduction in mass density. [less ▲]

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See detailCécité et fantastique dans rapport sur les aveugles d'Alberto Breccia
Denis, Benoît ULg; Ghizzardi, Yves

in Voir (1995), (11), 52-63

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See detailDiscussions about the Bp-d diagrams in polynomial for marine propeller series
Marchal, Jean ULg; Shen, Yide ULg; Kicheva, D.

in RINA (1995), volume 137

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See detailComment évaluer la sécrétion insulinique en pratique?
Scheen, A. J.; Paquot, Nicolas ULg; Letiexhe, M. R. et al

in Diabète & Métabolisme (1995), 21(6), 458-64

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See detailTolerance to Osmotic Shocks in Rats Kidney Cortex and Medulla
Gilles, Raymond ULg; Compère, Philippe ULg; el Goumzili, M. et al

in Tissue & Cell (1995), 27(6), 667-77

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic ... [more ▼]

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic shocks induces dramatic modifications in chromatin organization. The present paper reports on the changes of medulla cell chromatin in situ, in rat kidney slices submitted to osmotic challenges and in vitro, on preparations of extracted chromatin submitted to changes in environmental ion concentrations. Our results show that the chromatin of kidney medulla cells: (1) does not behave differently from the other mammalian chromatins when submitted in situ or in vitro to osmotic challenges; (2) presents in vitro physico-chemical characteristics similar to those of the other mammalian chromatins; and (3) is protected in vitro, as the other mammalian chromatins, from the disrupting effects of increases in inorganic ion concentrations by different compensatory organic solutes. The ability of kidney medulla cells to adapt to large increases in osmolarity could thus be related to a rapid control of the level of such compounds rather than to some rather specific, intrinsic molecular adaptations of macromolecules. [less ▲]

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See detailPlasma Membrane-Dependent Activation of Gelatinase a in Human Vascular Endothelial Cells
Lewalle, J. M.; Munaut, Carine ULg; Pichot, B. et al

in Journal of Cellular Physiology (1995), 165(3), 475-83

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the ... [more ▼]

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the matrix metalloproteinase family, degrades BM type IV collagen and is involved in the BM breakdown by migrating tumor cells and endothelial cells (EC). Gelatinase A is synthesized as latent proenzyme and must be activated in order to express its proteolytic activity. A plasma membrane-dependent mechanism of activation has been described for several tumor and transformed cells lines. In the present study, we show that latent (72 kD) and mature (62-59 kD) forms of gelatinase A are present in EC membrane fraction from Triton X-114 extract while only latent form is found in the cytosolic fraction. The incubation of EC membrane fraction with exogenous latent gelatinase A resulted in a significant activation giving rise to 62-59 kD mature forms. 12-O-tetradecanoylphorbol-13-acetate (TPA), a strong potentiator of angiogenesis in vitro and in vivo, increases the amount of both latent and activated forms of gelatinase A in EC membrane fraction as well as the ability of this latter fraction to activate exogenous latent gelatinase A. We show that the mRNA transcript coding for the membrane-integrated MMP, the MT-MMP, previously described as a potential gelatinase A activator in invasive tumor cells is also expressed in vascular EC and is regulated through a TPA sensitive process. This enzyme may be responsible for membrane-dependent gelatinase A activation in normal vascular EC and may therefore be a determinant in the control of BM proteolysis during angiogenesis. [less ▲]

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See detailIn vitro drug sensitivity and clinical aspects of Plasmodium falciparum malaria in African children
Carme, Bernard; Gay, Frederic; Ndounga, Mathieu et al

in Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (1995), 46(4), 270-274

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1 ... [more ▼]

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1: 39 asymptomatic school children, Group 2: 16 children with uncomplicated malaria, Group 3: 40 with severe but not pernicious malaria and Group 4: 20 with pernicious malaria. The drugs tested were chloroquine (CQ), quinine (QN) and mefloquine (MQ). The sensitivity level was assessed by a 48-hour in vitro maturation test involving the uptake of tritiated hypoxanthine, the initial blood level of parasite being > or = 0.1% in all cases. For QN and MQ, the median IC50 values showed no significant difference related to clinical status, age or parasitaemia levels. For CQ, the proportion of resistant strains and the 50 inhibitory concentration (IC50) values were greater in the cases of children hospitalised for malaria but there were no differences related to clinical severity of these hospitalised children nor, within each group, to the age or parasitaemia levels. The percentage of subjects with an IC50 value greater than the 90 percentile of the IC50 of the asymptomatic group, which we propose as the severity index related to chemoresistance, was 15% for uncomplicated malaria, 38% for severe but non-pernicious forms and 35% for pernicious malaria. The IC50 for QN was significantly higher in CQ-resistant strains and there was a positive correlation for CQ vs QN and for QN vs MQ. [less ▲]

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See detailBeef production in Belgium: to which extent manage extensification
Istasse, Louis ULg; Clinquart, Antoine ULg; Dufrasne, Isabelle ULg et al

in Proceeding of a workshop held in Paris on Novembre 22, 23 and 24, 1995 (1995)

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See detailGenetic Mapping of Mitochondrial Markers by Recombinational Analysis in Chlamydomonas Reinhardtii
Remacle, Claire ULg; Colin, Martine ULg; Matagne, René-Fernand ULg

in Molecular & General Genetics [=MGG] (1995), 249(2), 185-90

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type ... [more ▼]

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type minus (paternal) parent. In contrast mitotic zygotes transmit maternal and paternal mitochondrial DNA copies to their diploid progeny and recombinational events between molecules of both origins frequently occur. Six mitochondrial mutants unable to grow in the dark (dk- mutants) were crossed in various combinations and the percentages of wild-type dk+ recombinants were determined in mitotic zygotes when all progeny cells had become homoplasmic for the mitochondrial genome. In crosses between strains mutated in the COB (apocytochrome b) gene and strains mutated in the COX1 (subunit 1 of cytochrome oxidase) gene, the frequency of recombination was 13.7% (+/- 3.2%). The corresponding physical distance between the mutation sites was 4.3 kb. In crosses between strains carrying mutations separated by about 20 bp, a recombinational frequency of 0.04% (+/- 0.02%) was found. Two other mutants not yet characterized at the molecular level were also used for recombinational studies. From these data, a linear genetic map of the mitochondrial genome could be drawn. This map is consistent with the positions of the mutation sites on the mitochondrial DNA molecule and thereby validates the method used to generate the map. The frequency of recombination per physical distance unit (3.2% +/- 0.7% per kilobase) is compared with those obtained for other organellar genomes in yeasts and Chlamydomonas. [less ▲]

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See detailMutations Affecting the Mitochondrial Genes Encoding the Cytochrome Oxidase Subunit I and Apocytochrome B of Chlamydomonas Reinhardtii
Colin, Martine ULg; Dorthu, M. P.; Duby, Franceline ULg et al

in Molecular & General Genetics [=MGG] (1995), 249(2), 179-84

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase ... [more ▼]

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase pathway is still active the mutants have lost the capacity to grow heterotrophically (dark + acetate) and display reduced growth under mixotrophic conditions (light + acetate). In crosses between wild-type and mutant cells, the meiotic progeny only inherit the character transmitted by the mt- parent, which indicates that the mutations are located in the 15.8 kb linear mitochondrial genome. Two new mutants (dum-18 and dum-19) have now been isolated and characterized genetically, biochemically and at the molecular level. In addition, two previously isolated mutants (dum-11 and dum-15) were characterized in more detail. dum-11 contains two types of deleted mitochondrial DNA molecules: 15.1 kb monomers lacking the subterminal part of the genome, downstream of codon 147 of the apocytochrome b (COB) gene, and dimers resulting from head-to-head fusion of asymmetrically deleted monomers (15.1 and 9.5 kb DNA molecules, respectively). As in the wild type, the three other mutants contain only 15.8 kb mitochondrial DNA molecules. dum-15 is mutated at codon 140 of the COB gene, a serine (TCT) being changed into a tyrosine (TAC). dum-18 and dum-19 both inactivate cytochrome c oxidase, as a result of frameshift mutations (addition or deletion of 1 bp) at codons 145 and 152, respectively, of the COX1 gene encoding subunit I of cytochrome c oxidase. In a total of ten respiratory deficient mitochondrial mutants characterized thus far, only mutations located in COB or COX1 have been isolated.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 20. Effect of monomer, solvent, and initiator on the ring-opening polymerization as initiated with aluminum alkoxides
Ropson, Nathalie; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(23), 7589-7598

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization ... [more ▼]

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization initiated by aluminum alkoxides. In toluene, aluminum triisopropoxide, a commonly used initiator, forms tetrameric (A(4)) and/or trimeric (A(3)) aggregates, which are dissociated upon the addition of the cyclic monomer. Actually, LA is responsible for the complete deaggregation of both A(3) and A(4) into single species (A(1)) trisolvated by the monomer (Al coordination number, CN = 6), whereas A(3) is selectively deaggregated by epsilon-CL. This behavior is consistent with the higher stability of A(4) compared to A(3). Whatever the monomer, three chains are initiated by the active A(1) species. When conversion of epsilon-CL is complete, the active aluminum trialkoxide sites are solvated by one (CN = 4) and more probably three (CN = 6) carbonyl groups of the attached poly-epsilon-caprolactone chains. At the complete LA conversion, only one carbonyl group of the polylactide chains is coordinated to Al (CN = 4). In THF, there is a competition between monomer and solvent for coordination to Al, which explains why polymerization is slower in THF than in toluene. In both toluene and THF, diethylaluminum monoalkoxide forms cyclic trimers that are solvated by the monomer (epsilon-CL) at least on the NMR time scale. Nevertheless, a fractional kinetic order in initiator is observed which gives credit to a fast reversible dissociation of the epsilon-CL-solvated trimers. [less ▲]

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See detailPhase-shifting real time interferometry that uses bismut silicon oxide crystals
Georges, Marc ULg; Lemaire, Philippe

in Applied Optics (1995), 34(32), 7497-7506

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with ... [more ▼]

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with an interferogram-analysis method that uses the phase-shifting technique for quantitative measurement of diffusive-reflecting object deformations. In addition to the usual error sources in phase shifting, the temporal interferogram erasure is studied and is found weakly perturbative for the measured phase. Itis shown that quantitative measurements are possible for low-intensity object beams (8 μW/cm2) and a large observed area. Apractical situation of defect monitoring in a composite structure is presented. [less ▲]

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See detailThe Replication in Vitro of the Gammaherpesvirus Bovine Herpesvirus 4 Is Restricted by Its DNA Synthesis Dependence on the S Phase of the Cell Cycle
Vanderplasschen, Alain ULg; Goltz, M.; Lyaku, J. et al

in Virology (1995), 213(2), 328-40

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to ... [more ▼]

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to determine the relationship between BHV-4 infection and the cell cycle. The temporal expression of BHV-4 late (L) proteins in unsynchronized cell cultures was first investigated by flow cytometry. Interestingly, L protein expression occurred in a limited number of cells infected with a high multiplicity of infection, and a reciprocal correlation between the percentage of positive cells and the cell density at the time of infection was demonstrated. Moreover, the finding that a BHV-4 early-late protein was expressed in nearly all the cells suggested that a blockage in the viral replication cycle occurred in some infected cells at the stage of viral DNA synthesis or L protein expression. Because this blockage could be the consequence of the dependence of one or both of these events on the cell cycle, they were investigated after infection of synchronized cell cultures. The following findings were made. (i) Cell transition through the S phase quantitatively increased the rate of BHV-4 DNA replication. (ii) BHV-4 DNA synthesis could not be detected in cells arrested in G0. (iii) Synchronization of MDBK cells with Lovastatin before infection increased the percentage of cells expressing L proteins. (iv) In contrast, infection of cells arrested in G0 led to few positive cells. Taken together these results showed that BHV-4 DNA replication and consequently the expression of L proteins are dependent on the S phase of the cell cycle. This dependence could be of importance for several biological properties of BHV-4 infection in vitro and might have implications for the biology of the virus in vivo. [less ▲]

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See detailHighly-Expressed P100/P52 (Nfkb2) Sequesters Other Nf-Kappa B-Related Proteins in the Cytoplasm of Human Breast Cancer Cells
Dejardin, Emmanuel ULg; Bonizzi, Giuseppina; Bellahcene, Akeila ULg et al

in Oncogene (1995), 11(9), 1835-41

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the ... [more ▼]

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the molecular control of the transformed phenotype, we examined the expression of these proteins in human breast cancer cell lines as well as in primary tumors. Western Immunoblots demonstrated high expression of the p52 precursor p100 (NFKB2) in several breast cancer cell lines while human mammary epithelial cells express this protein only faintly. Eighteen primary breast tumors out of 24 displayed significant expression of the p100/p52 protein. In MDA-MB-435 cells, overexpressed p100 and p52 are predominantly cytoplasmic and coimmunoprecipitation experiments demonstrated that p100 sequesters the heterodimer p50/p65 in the cytoplasm. We demonstrate that most p65 protein is complexed with p100 in these cells while it is complexed predominantly with I kappa B-alpha in cell lines expressing less p100. Our data strengthen the hypothesis that NF-kappa B could be involved in carcinogenesis and suggest that the p100/p52 NF-kappa B subunit could play a role in the development of human breast cancers, possibly by sequestering other NF-kappa B-related proteins in the cytoplasm. [less ▲]

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See detailStellar calibration of CCD flat fielding.
Manfroid, Jean ULg

in Astronomy and Astrophysics. Supplement Series (1995), 113

The role of star observations is advocated in the photometric calibration of the low spatial-frequency non-uniformities of CCD detectors.

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See detailPhotometric monitoring (1987 to 1994) of the gravitational lens candidate UM 425.
Courbin, F.; Magain, Pierre ULg; Remy, M. et al

in Astronomy and Astrophysics (1995), 303

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged ... [more ▼]

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged through an R filter in order to obtain their light curves. The photometry was obtained by simultaneously fitting a stellar two-dimensional profile on each component. The brightest image (component A, m_R_=15.7) shows a slow and smooth increase in brightness of 0.2 magnitude in seven years, while the faintest one (component B, m_R_=20.1) displays an outburst of 0.4 magnitude which lasts approximately two years. The variation of component B may be interpreted in two ways, assuming UM 425 is gravitationally lensed. If it is due to an intrinsic variation of the quasar, we derive a lower limit of 3 years on the time delay from the fact that it is not observed in component A. On the other hand, if it is a microlensing "High Amplification Event", we estimate the size of the source to be ~10^-3^pc, in agreement with standard models of AGNs. These observations are consistent with the gravitational lens interpretation of the object. Furthermore, all the CCD frames obtained under the best seeing conditions have been co-added, in an attempt to detect the deflector. The final R image reveals a rich field of faint galaxies in the magnitude range m_R_~22-24. No obvious deflector, nor any system of arcs or arclets is detected, down to a limiting magnitude of m_R_~24. [less ▲]

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See detailEffect of naloxone and metoclopropamide on LH secretion in a hyperprolactinemic, hypogonadotropic, postmenopausal woman, fertility and sterility.
Verhelst, J.; Beckers, Albert ULg; Abs, R.

in Fertility and Sterility (1995), 64(5), 969-971

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year ... [more ▼]

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year-old woman with a macroprolactinoma. INTERVENTIONS: Serial 10-hour IV infusions of naloxone and metoclopramide. MAIN OUTCOME MEASURE: Serum LH concentration. RESULTS: Naloxone induced a small but significant rise of serum LH levels, which displayed a pulsatile pattern. By contrast, metoclopramide elicited no significant response in LH secretion. CONCLUSION: Opioidergic but not dopaminergic neurotransmission plays a direct role in the suppression of LH secondary to hyperprolactinemia. [less ▲]

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See detail"Music alone survives”? The collapse of faith in sonnets by Geoffrey Hill and G.M. Hopkins’
Pagnoulle, Christine ULg

in Cahiers Victoriens & Edouardiens (1995), 42

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's ... [more ▼]

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's most famous celebratory sonnet 'The Windhover.' [less ▲]

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See detailFactors influencing the immediate and late outcome of cushing-disease treated by transsphenoidal surgery : A retrospective study by the european cushings-disease survey group
Bochicchio, D.; Losa, M.; Buchfelder, M. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3114-3120

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of ... [more ▼]

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of patients in order to identify the prognostic factors influencing the outcome after transsphenoidal surgery. We conducted a multicenter, retrospective analysis of the early and late results of surgical treatment of Cushing's disease. Files of patients with Cushing's disease who underwent transsphenoidal surgery between 1975 and 1990 were collected from 25 institutions throughout Europe. Data from 668 of 716 patients were suitable for statistical analyses. Surgical mortality was 1.9%, and major morbidity occurred in 97 patients (14.5%). Clinical and biochemical remission of Cushing's disease after surgery occurred in 510 cases (76.3%). Identification of the tumor by neuroradiological imaging or at operation with histopathological corroboration was associated with remission of hypercortisolism. Recurrence of the disease occurred in 65 (12.7%) of 510 patients in remission after surgery at a mean time of 39.3 months (range 6-104 months). The distribution of the recurrences did not show any apparent plateau or cluster throughout the follow-up period. Low postoperative steroid levels, absence of cortisol response to CRH, and the need for long-term glucocorticoid substitution therapy were all associated with a high probability of long-term remission. Our study demonstrates that transsphenoidal surgery is a safe and effective treatment for patients with Cushing's disease. However, after successful surgery there is a steady increase in the percentage of recurrences, which continues with time. Patients who after operation had hypoadrenocorticism and needed long-term glucocorticoid substitution therapy had the lowest risk of relapse. [less ▲]

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See detailPreparation of a Macroporous Biodegradable Polylactide Implant for Neuronal Transplantation
Schugens, C.; Grandfils, Christian ULg; Jérôme, Robert ULg et al

in Journal of Biomedical Materials Research (1995), 29(11), 1349-62

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an ... [more ▼]

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an original method consisting of the aggregation of calibrated poly-D,L-lactide microparticles. The size of the interstices between the aggregated microspheres was in a direct relationship to the microsphere diameter. A first approach was based on coating the microspheres with poly(vinyl alcohol) followed by chemically crosslinking the coating layers that were in mutual contact. This method was disregarded because of the acute cytotoxicity of glutaraldehyde used as the crosslinking agent, the absence of macroporosity, and the complete lack of flexibility. A physical technique of aggregation was then tested, which relied on the plasticization of poly-D,L-lactide microspheres with triethylcitrate to the point where microspheres strongly adhered to each other when they were in contact. This method has proved to be straightforward and definitely superior to the chemical approach, particularly with respect to cytotoxicity, control of macroporosity, and flexibility. A polymer support was thus successfully which was biodegradable, macroporous( interconnected pores of 10-100 microns in diameter), and flexible. This potential medical device is presently being used for neuronal transplantation in the central nervous system. [less ▲]

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See detailLe cas clinique du mois. Dépression majeure chez un patient diabétique
Pitchot, William ULg; Ansseau, Marc ULg

in Revue Médicale de Liège (1995), 50(11), 451-2

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See detailEfficacy and Safety of Drugs for Paget's Disease of Bone
Reginster, Jean-Yves ULg; Lecart, M. P.

in BONE (1995), 17(5 Suppl), 485-488

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and ... [more ▼]

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and bisphosphonates are now considered as the main therapeutic approaches for this disease. Daily parenteral administration of calcitonin to patients with Paget's disease of bone results in a significant fall in serum alkaline phosphatase and urinary hydroxyproline levels. This treatment has also been reported to be effective in relieving clinical symptoms of the disease, mainly bone pain. The drawbacks of injectable calcitonin have stimulated interest in alternative routes of delivery. Substantial evidence of calcitonin bioavailability and bioefficacy equivalent to those of parenteral administration is currently available for only two alternative routes: nasal spray and rectal suppository. Since many results have been published showing a dramatic effect of several bisphosphonates in Paget's disease of bone, nasal and rectal calcitonin are no longer considered as the treatments of choice in this condition. A major advantage of the use of bisphosphonates over calcitonin in Paget's disease is that biochemical and histologic suppression of disease activity may persist for many years after the cessation of treatment. Oral etidronate and intravenous pamidronate have been extensively used and have provided satisfactory benefits to the patient. Since the risk/benefit ratio of alendronate does not appear to be completely positive, it is likely that the future of treatment of Paget's disease of bone will be based on the oral formulation of the new bisphosphonates, including tiludronate, risedronate or dimethyl-pamidronate. [less ▲]

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See detailIs Sepsis a Mediator-Inhibitor Mismatch?
Lamy, Maurice ULg; Deby, Ginette ULg

in Intensive Care Medicine (1995), 21(Suppl 2), 250-7

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See detailThe prevalence of colonic polyps in acromegaly : a prospective colonoscopic and pathological study in 103 patients.
Delhougne, B.; Deneux, C.; Abs, R. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3223-3226

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results ... [more ▼]

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results obtained in rather small series of patients or without a control group. We, therefore, undertook a prospective colonoscopic and pathological study comprising 103 acromegalic patients and 138 nonacromegalic control subjects referred for irritable bowel syndrome. The prevalence of adenomatous colonic polyps was significantly increased in acromegalic patients compared to that in control subjects (22.3% vs. 8.0%; P = 0.0024). The significance was similarly present in male acromegalic patients (28.6% vs. 5.5% in male control subjects; P = 0.0026), but was absent in female acromegalic patients. The prevalence of colonic polyps was also significantly increased in the group of acromegalic patients under 55 yr of age (20.0% vs. 3.0% in the control group of the same age; P = 0.0026). Other characteristics of adenomatous colonic polyps in acromegaly were the multiplicity and the presence proximal to the splenic flexure. No difference in the duration of acromegaly was found between patients with or without adenomatous polyps. The prevalence of hyperplastic colonic polyps was also significantly increased to 24.3% in acromegalic patients vs 4.4% in control subjects (P < 0.001). In conclusion, in view of the increased incidence of adenomatous colonic polyps, colonoscopy should be part of the follow-up examination in acromegaly. [less ▲]

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See detailEvaluation and comparison of the behaviour at sea and forces in cables of two types of offshore tension leg platforms
Marchal, Jean ULg; Lejeune, A.

in International Symposium on CABLE DYNAMICS (1995)

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See detailRetinoic Acid Induces Three Newly Cloned Hoxa1 Transcripts in Mcf7 Breast Cancer Cells
Chariot, Alain ULg; Moreau, Louis; Senterre, Geoffrey et al

in Biochemical and Biophysical Research Communications (1995), 215(2), 713-20

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of ... [more ▼]

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of their cDNA sequences are still unknown. We report here the molecular characterization of three newly cloned HOXA1 transcripts from human breast cancer cells. In addition, we provide evidence that these alternatively spliced transcripts encode one homeodomain-containing protein and two products lacking the conserved DNA-binding domain. Moreover, we demonstrate that all three HOXA1 transcripts are induced by retinoic acid in MCF7 cells. Taken together, our results suggest that HOXA1 gene may be a key element in the establishment of the breast cancer cell phenotype. [less ▲]

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See detailAnionic polymerization of pivalolactone initiated by alkali metal alkoxides
Jedlinski, Zbigniew; Kurcok, Piotr; Kowalczuk, Marek et al

in Macromolecules (1995), 28(21), 7276-7280

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the ... [more ▼]

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the initiator, onto the carbonyl carbon atom of the monomer the selective cleavage of the acyl-oxygen bond of the monomer leads to the formation of alkoxide propagating species. Thus, it has been demonstrated that, in contrast to alpha-unsubstituted beta-lactones and higher lactones, the anionic polymerization of pivalolactone proceeds through either alkoxide or carboxylate propagation centers, depending on the nature of initiator used, i.e. an alkoxide or a carboxylate, respectively. In the former case, however, cyclic oligomers are also formed which are unusual in the anionic polymerization of other beta-lactones. This indicates that, whatever the lactone ring size (four-, six-, or seven-membered lactones), intramolecular transesterification reactions can take place if alcoholate ions are propagating species. [less ▲]

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See detailLong-term photometry of variables at ESO. IV. The fourth data catalogue (1992-1994).
Sterken, C.; Manfroid, Jean ULg; Beele, D. et al

in Astronomy and Astrophysics. Supplement Series (1995), 113

In this paper we present the fourth catalogue of photometric data in the Stroemgren system obtained during the period June 18, 1992-August 23, 1994 in the framework of the Long-Term Photometry of ... [more ▼]

In this paper we present the fourth catalogue of photometric data in the Stroemgren system obtained during the period June 18, 1992-August 23, 1994 in the framework of the Long-Term Photometry of Variables (LTPV) program at the European Southern Observatory. The catalogue is available in computer readable form at the Centre de Donnees de Strasbourg via anonymous ftp 130.79.128.5. [less ▲]

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See detailLack of universality in 2-dimensional multicomponent spreading phenomena
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1995), 52(4), 3447-3454

Magnetic entities are introduced in a two-dimensional Eden model as additional degrees of freedom in order to model multidomain spreading phenomena. The elements of the growth are "spins" taking q states ... [more ▼]

Magnetic entities are introduced in a two-dimensional Eden model as additional degrees of freedom in order to model multidomain spreading phenomena. The elements of the growth are "spins" taking q states and are coupled or not via an energy J, as in the Potts model, thus leading to a competition between species. The internal cluster spreading is found to result from a competition between growing domains. Complex mechanisms such as trapping, jamming and coalescence occur between the growing domains. A large variety of critical and nanuniversal regimes, from subcritical to self-organized critical behaviors, are obtained depending on nonuniversal parameters such as the lattice structure, the number of internal degrees of freedom q, and the coupling J. For the square lattice, the fractal dimension is 1.50 and the mass distribution exponent tau is 1.63. For the triangular lattice, the fractal dimension varies from 1.70 to 1.83 depending on the coupling value and the mass distribution exponent tau also varies from 1.67 to 1.98 depending on the coupling value. The correspondence and differences with respect to the percolation phenomenon are outlined. [less ▲]

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See detailEffect of Plasma Anticonvulsant Level on Pipecuronium-Induced Neuromuscular Blockade: Preliminary Results
Hans, Pol ULg; Ledoux, Didier ULg; Bonhomme, Vincent ULg et al

in Journal of Neurosurgical Anesthesiology (1995), 7(4), 254-8

Patients receiving anticonvulsants are resistant to nondepolarizing muscle relaxants (NDMR). This study examines the effect of plasma anticonvulsant levels on pipecuronium-induced neuromuscular blockade ... [more ▼]

Patients receiving anticonvulsants are resistant to nondepolarizing muscle relaxants (NDMR). This study examines the effect of plasma anticonvulsant levels on pipecuronium-induced neuromuscular blockade. Twenty adult patients scheduled for neurosurgery were assigned to one of two groups. Group 0 (G0) consisted of 10 patients not on anticonvulsant therapy; group 1 (G1) included 10 patients treated either with phenytoin or carbamazepine. G1 patients were further divided into G1u (n = 4) and G1w (n = 6) subgroups, according to the plasma anticonvulsant level measured the day before surgery--under (G1u) or within (G1w) the therapeutic range. Neuromuscular transmission was monitored with a Biometer International A/S Accelograph. Anesthesia was induced and maintained using propofol and sufentanil. After calibration of the accelograph, a bolus of pipecuronium 0.08 mg/kg was given IV. The time from pipecuronium injection to the peak reduction of T1 was taken as the onset time. The time in min from pipecuronium injection to recovery of T1% (first accelograph response/baseline response) x 100 and TR% (fourth accelograph response/first accelograph response) x 100 were recorded at 25, 50, and 75% of baseline. The recovery index (RI) was taken as the time from 25 to 75% of baseline. The recovery index (RI) was taken as the time from 25 to 75% recovery of the baseline response. The onset time was not different in G0 (203 +/- 60.4 s), G1 (230.5 +/- 79.3 s), and G1u (181.8 +/- 60.4 s) but prolonged in G1w (279.2 +/- 67.7 s).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLes cereales dans l'alimentation des bovins: valeur nutritionnelle et recommandations pour une utilisation sans risque.
Thewis, André ULg; Decruyenaere, Virginie

in Elevages Belges (1995), 49(10), 9-12

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See detailLumière and his View : the Cameraman's Eye in Early Cinema
Belloi, Livio ULg

in Historical Journal of Film, Radio & Television (1995), 15

Analyse des scènes de rue produites par la firme Lumière comme résultats d'une interaction entre l'opérateur et les sujets filmés.

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See detailThe Impact of Renal Revascularisation on Renal Dysfunction
Van Damme, Hendrik ULg; Jeusette, F.; Pans, Alain ULg et al

in European Journal of Vascular and Endovascular Surgery : The Official Journal of the European Society for Vascular Surgery (1995), 10(3), 330-7

AIM: To determine the value of kidney revascularisation in patients with impaired renal function and correctable renal artery stenosis, the authors reviewed their surgical experience from 1978 to 1990 ... [more ▼]

AIM: To determine the value of kidney revascularisation in patients with impaired renal function and correctable renal artery stenosis, the authors reviewed their surgical experience from 1978 to 1990. PATIENTS AND METHODS: The study population included 23 patients with ischaemic nephropathy whose preoperative baseline creatinine level exceeded 20 mg/l (range 21-65 mg/l). This represents 20% of all patients operated on for renal artery disease during the same time interval. Preoperative risk profile, operative mortality, impact on hypertension and on renal function, and late survival were analysed. Renal function response to kidney revascularisation was defined as favourable (20% or more reduction of serum creatinine), moderate (stabilised serum creatinine values) or bad (further deterioration of renal function). All patients had atherosclerotic renal artery disease, involving a solitary kidney in five, both kidneys in 15 and one of the two kidneys in three patients. Hypertension was present in 74%. Revascularisation was unilateral in 10, bilateral in nine and associated with controlateral nephrectomy in four patients. RESULTS: Four patients died postoperatively (three myocardial infarctions, one stroke). Four patients needed postoperative short-term dialysis. After operation, renal function improved in 13, stabilised in six and deteriorated in four patients (of whom two died). Follow-up among the survivors averaged 46 months. The mean serum creatinine value at last follow-up visit was 26.2 mg/l, a decrease of 7.7 mg/l compared to preoperative values (p < 0.05). Overall, 69% of azotemic patients submitted to renal revascularisation manifested a favourable response (45% improved and 24% stabilised). Three patients required long-term dialysis. The 5-year survival rate was 48%. CONCLUSION: These data suggest that kidney revascularisation in patients with ischaemic nephropathy can restore or stabilise renal function, preventing evolution and end-stage renal disease and dialysis dependency. [less ▲]

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See detailAntioxidant Defense and Free Radical Production in a Rabbit Model of Kidney Ischemia-Reperfusion
Franssen, Colette ULg; Defraigne, Jean-Olivier ULg; Detry, Olivier ULg et al

in Transplantation Proceedings (1995), 27(5), 2880-3

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See detailExercise in the Management of Non-Insulin-Dependent (Type 2) Diabetes Mellitus
Gautier, J. F.; Scheen, André ULg; Lefebvre, P. J.

in International Journal of Obesity & Related Metabolic Disorders (1995), 19(Suppl 4), 58-61

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See detailAdsorption behavior of polymers with ionic end groups : mono- and difunctional polystyrenes in toluene
Pankewitsch, T.; Vanhoorne, Pierre ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(20), 6986-6992

The adsorption behavior of mono- and difunctional telechelic polymers, omega- and alpha,omega-sulfonato polystyrenes, from dilute toluene solution on the surface of a silicon wafer is investigated by ... [more ▼]

The adsorption behavior of mono- and difunctional telechelic polymers, omega- and alpha,omega-sulfonato polystyrenes, from dilute toluene solution on the surface of a silicon wafer is investigated by ellipsometry. Polystyrene without ionic end groups does not adsorb, and the attachment of molecules to the silicon dioxide surface proceeds via the functional ionic ends. Already a single ionic group promotes adsorption, and adsorption kinetics at small times is found to be diffusion limited. The approach of an equilibrium adsorbed amount at later times is controlled by the hindered diffusion of chains through the already existing layer. With monofunctional chains a thin stable monolayer at the surface is formed. With difunctional chains there is strong indication for coupling of ionic ends and the formation of additional layers. It is caused by the interaction of dangling ends, when only one end of the chains is adsorbed at the surface. The molecular weight dependence of the grafting density is consistent with scaling predictions, and differences in the adsorbed amount of telechelics with different counterions are explained on the basis of different valencies. [less ▲]

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See detailSmall-angle neutron scattering studies of low-polarity telechelic ionomer solutions. 1. Total scattering
Karayianni, Eleni; Jérôme, Robert ULg; Cooper, Stuart L

in Macromolecules (1995), 28(19), 6494-6507

The overall structure and thermodynamic interactions that take place,in low-polarity ionomer solutions have been investigated via the technique of small-angle neutron scattering for two Na-neutralized ... [more ▼]

The overall structure and thermodynamic interactions that take place,in low-polarity ionomer solutions have been investigated via the technique of small-angle neutron scattering for two Na-neutralized carboxy-telechelic polystyrene model ionomer systems that differ in the length of the polymer backbone. The strong interchain association that is evidenced in the viscometric measurements of the ionomers compared to the ester forms is apparent in the scattering profiles of the ionomers that show a characteristic upturn that extends in the low-q regime and increases with increasing polymer concentration. The apparent molecular weight and size of the aggregated particles show a significant increase over a small concentration range, while the ionic content seems to not affect the degree of association in the concentration regime studied. The supramolecular structure of the ionomer solutions is revealed by fitting the scattering data to a scaling law in the intermediate qR(g) regime that supports a percolation type of behavior for the ionomer clusters for both systems studied. The fractal dimension of the aggregates is 1.76 for both ionomer systems which provides evidence for a rather extended configuration and high polydispersity of the clusters in solution. The molecular weight of the associating particles is observed to rapidly approach infinity with increasing polymer concentration, indicating the approach to a gel transition. The scattering data of the ionomer solutions were fitted to the de Gennes model based on the random-phase approximation theory. The Flory-Huggins interaction parameter of the ionomer solutions determined using this model shows a strong concentration dependence with a quite unexpected increase with decreasing polymer concentration. The thermodynamic interactions in the ionomer solutions were changed dramatically by the association as revealed by a significant decrease of the second virial coefficient. [less ▲]

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See detailEspace et mémoire
Frankignoulle, Pierre ULg

in Le Magazine Liège Université (1995)

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See detailSystemic Immune Response after Rectocolonic Administration of Ovalbumin in Mice
Louis, Edouard ULg; Franchimont, D.; Lamproye, Anne ULg et al

in International Archives of Allergy & Immunology (1995), 108(1), 19-23

The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c ... [more ▼]

The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c mice. One rectocolonic, but not intragastric, administration of 25 mg of ovalbumin induced a detectable increase in serum anti-ovalbumin antibody level. The level reached was however much lower than after subcutaneous injection. Both intragastric and rectocolonic immunization with ovalbumin induced specific systemic cellular tolerance. However, after rectocolonic, but not intragastric, preimmunization there was no systemic humoral tolerance to this antigen. These differences in systemic immune responses after rectocolonic or intragastric administration of ovalbumin could be due to different stimulation of the systemic immune system or to differences between the colonic and small bowel mucosal immune system, which remain to be elucidated. [less ▲]

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See detailHypertension artérielle et résistances vasculaires
Krzesinski, Jean-Marie ULg; Rorive, Georges ULg

in Revue Médicale de Liège (1995), 50(9), 365-372

L'anomalie hémodynamique caractéristique de l'hypertension artérielle est l'augmentation des résistances vasculaires. Ces résistances siègent principalement au niveau de petites artères et d'artérioles ... [more ▼]

L'anomalie hémodynamique caractéristique de l'hypertension artérielle est l'augmentation des résistances vasculaires. Ces résistances siègent principalement au niveau de petites artères et d'artérioles précapillaires de diamètre inférieur à 300Il et sont le résultat de modifications essentiellement anatomiques. La média est de loin la couche la plus épaisse de la paroi de ces vaisseaux caractérisés,au stade d'hypertension chronique, par un rapport épaisseur de média sur lumière accru. Contrairement à l'animal hypertendu, cette modification est, chez l'homme, liée à un remodelage vasculaire (réarrangement cellulaire sans vraie hypertrophie ou hyperplasie), en relation avec une anomalie endothéliale (réduction de production de monoxyde d'azote, augmentation de production d'endothéline-l). L'intérêt pour ces modifications structurelles réside dans l'espoir d'influencer favorablement ces lésions notamment par des traitements spécifiques. Récemment, une étude canadienne a rapporté qu'un inhibiteur de l'enzyme de conversion, à même efficacité antihypertensive qu'un bêtabloquant, était seul capable de normaliser les anomalies structurelles de la média et de corriger (partiellement) la dysfonction endothéliale caractérisant également l'hypertendu après 27ms de traitement (étude chez l'homme, à partir de biopsies répétées de tissu sous-cutané, selon la technique proposée par les groupes de Heagerty et Mulvany). Cette observation jointe aux données expérimentales et à quelques données humaines suggère que les inhibiteurs de l'enzyme de conversion posséderaient bien des propriétés indépendantes de leur effet antihypertenseur permettant une correction des anomalies vasculaires dans l'hypertension. Ces travaux méritent cependant une confirmation sur un grand nombre de patients. Ils ont l'intérêt d'ajouter une note explicative pathogénique aux bénéfices déjà observés en épidémiologie dans la décompensation cardiaque, l'infarctus myocardique ou la protection rénale lors de l'utilisation de ces molécules. [less ▲]

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See detailThe kinetic growth anisotropy of the 123-(RE)BaCuO compounds
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Philosophical Magazine A (1995), 72(3), 727-736

The kinetic growth anisotropy of the melt-textured 123-(RE)BaCuO compounds, especially along the CuCO2 planes, is discussed here in detail. A computer model which simulates the grain growth of 123 ... [more ▼]

The kinetic growth anisotropy of the melt-textured 123-(RE)BaCuO compounds, especially along the CuCO2 planes, is discussed here in detail. A computer model which simulates the grain growth of 123 materials in the ab plane is presented. This kinetic growth model takes some bond anisotropy along the [100] and [110] directions into account. The model presented herein belongs to a hierarchical set of generalized Eden models as discussed in the Appendix. For different bond anisotropy parameters, the simulated 123-(RE)BaCUO grains exhibit square and octogonal overall shape which have been both optically observed. From simple kinetic arguments, we show that the 'faceting' of the 123-(RE)BaCuO grains finds its origin in a bond anisotropy rather than in a growth-rate anisotropy along some crystallographic directions. This is of general interest and opens new perspectives for developing the Eden model in the crystal-growth field. [less ▲]

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See detailOn the electrochemical polymerization of acrylonitrile and N-vinylpyrrolidone : new insight into the mechanism
Jérôme, Robert ULg; Mertens, Marc; Martinot, Lucien

in Advanced Materials (1995), 7(9), 807-809

The protection of metals against environmental attack can be achieved by coating the metal with a protective organic film. Poor adhesion of the organic film to the metal surface has, however, limited the ... [more ▼]

The protection of metals against environmental attack can be achieved by coating the metal with a protective organic film. Poor adhesion of the organic film to the metal surface has, however, limited the success of this approach. Here it is demonstrated that polyacrylonitride can be elecrografted onto a nickel surface producing a strongly adherent film. The transformation of the organic film could also open the way to new polymer-metal composites. [less ▲]

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See detailLe cas clinique du mois. Rémission spontanée prolongée d'un diabète de type 1 typique
Scheen, A. J.; Letiexhe, M. R.; Paquot, Nicolas ULg et al

in Revue Médicale de Liège (1995), 50(9), 363-4

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See detailIncidence and direct social costs of hip fractures from 1984 to 1993 in Belgium
Gillet, Pierre ULg; Reginster, Jean-Yves ULg

in Journal of Bone and Mineral Research (1995)

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See detailCytogenetic study of bovine oocytes matured in vitro
Ectors, Fabien ULg; Koulischer, Lucien ULg; Jamar, M. et al

in Theriogenology (1995), 44(3), 445-450

Described in the present paper is a cytogenetic study of bovine oocytes matured in vitro. The cumulus-oocyte complexes (COC), punctured from ovaries recovered in a local slaughterhouse, were classified ... [more ▼]

Described in the present paper is a cytogenetic study of bovine oocytes matured in vitro. The cumulus-oocyte complexes (COC), punctured from ovaries recovered in a local slaughterhouse, were classified into 3 groups according to follicular diameter 1 to 4mm, 5 to 8mm and 9 to 13 mm. Metaphases available for observation were classified as metaphase I, haploid and diploid metaphase II. High levels of haploid metaphases II (90.6, 86.9 and 94.4 %) among the 3 groups of follicular sizes indicated successful meiotic resumption during in vitro maturation and suggested that cytoplasmic maturation may be responsible for low developmental rate after IVM, IVF and in vitro development (IVD). [less ▲]

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See detailSelf-organized criticality in phylogenetic-like tree growths
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Journal de Physique I (1995), 5(8), 1011-1025

A simple stochastic model of Darwinistic evolution generating phylogenetic-like trees is developed. The model is based on a branching process taking competition-correlation effects into account. In ... [more ▼]

A simple stochastic model of Darwinistic evolution generating phylogenetic-like trees is developed. The model is based on a branching process taking competition-correlation effects into account. In presence of finite and short range correlations, the process self-organizes into a critical steady-state in which intermittent bursts of activity of all sizes are generated. On a geological-like time scale, this behaviour agrees with punctuated equilibrium features of biological evolution. The simulated phylogenetic-like trees are found to be self-similar. The dynamics of the transient regimes show a power law decrease of the order parameter towards the 0(+) value which characterizes an unstable critical state. The genetic range k of competition-correlations between living species is found to be a relevant parameter which determines the universality class of the evolution process. An infinite competition-correlation range destroys however the self-organized critical behaviour. The fractal dimension D-f of the phylogenetic-like trees increases from 2.0 to infinity as k goes from 1 to infinity. The critical exponent tau of avalanche size-distribution decreases from about 3/2 (for k = 1) and reaches about 1.2 for k = 10. A hyperscaling relation seems to relate the various universality classes. Through a mean-field theory, we mention that the evolution process is much more complex than a simple uncorrelated branching process. [less ▲]

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See detailPharma-clinics. Le médicament du mois. La terbinafine (Lamisil)
Pierard, Gérald ULg

in Revue Médicale de Liège (1995), 50(8), 352-3

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