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See detailIsolation of the missing 5-end of the encoding region of the bovine leukemia virus cell receptor gene.
Ban, J.; Truong, A. T.; Horion, B. et al

in Archives of Virology (1994), 138(3-4),

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See detailAsthme et hyperréactivité bronchique non spécifique
Louis, Renaud ULg; Kayembe, J. M.; Radermecker, Maurice ULg

in Acta Clinica Belgica (1994), 49(3-4), 148-57

Bronchial hyperresponsiveness is a hallmark of asthma although it can also be present, to a lesser extent, in other diseases. The level of bronchial responsiveness depends on immuno-inflammatory processes ... [more ▼]

Bronchial hyperresponsiveness is a hallmark of asthma although it can also be present, to a lesser extent, in other diseases. The level of bronchial responsiveness depends on immuno-inflammatory processes modifying the functional status of airway smooth muscle as well as the structure of the bronchial wall. The responsiveness toward a direct constricting pharmacological agent is poorly correlated to the one toward an indirect constricting agent or a physical stimulus which cause airway obstruction through a more complex mechanism. Transversal studies show a relationship between the severity of asthma and the level of methacholine airway responsiveness. Long term treatment with corticoids can reduce the bronchial hyperresponsiveness of asthmatics. [less ▲]

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See detailAN IMPROVED ONE-POINT INTEGRATION METHOD FOR LARGE-STRAIN ELASTOPLASTIC ANALYSIS
Stainier, Laurent ULg; Ponthot, Jean-Philippe ULg

in Computer Methods in Applied Mechanics & Engineering (1994), 118(1-2), 163-177

In this paper, we present a new one-point integration method generalizing Flanagan and Belytschko's method (Internat. J. Numer. Methods Engrg. 17 (1981) 679-706) and a modification of Belytschko and ... [more ▼]

In this paper, we present a new one-point integration method generalizing Flanagan and Belytschko's method (Internat. J. Numer. Methods Engrg. 17 (1981) 679-706) and a modification of Belytschko and Bindeman's method (Comput. Methods Appl. Mech. Engrg. 88 (1991) 311-340), both in the frame of large deformation elastoplastic analysis. These stabilization methods are combined with the radial return method used to integrate the constitutive law. Plane strain problems are first considered, and the method is then generalized to axisymmetrical situations. The explicit time integration scheme with its critical timestep is also considered. A few examples are presented that show the great time savings that can be obtained with reduced integration without any loss of accuracy, and even with a gain in the solution quality, since the underintegrated elements prove to be 'flexurally superconvergent'. [less ▲]

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See detailEffect of recombinant human erythropoietin on platelets in patients with anemia of renal failure: correlation of platelet count with erythropoietic activity and iron parameters.
Beguin, Yves ULg; Loo, Martine; R'Zik, Samir ULg et al

in European Journal of Haematology (1994), 53(5), 265-70

We examined the effect of treatment with rHuEpo on platelet counts in 61 hemodialysis patients and correlated them with changes in erythropoietic activity, iron status and inflammation. Platelets (10(9)/1 ... [more ▼]

We examined the effect of treatment with rHuEpo on platelet counts in 61 hemodialysis patients and correlated them with changes in erythropoietic activity, iron status and inflammation. Platelets (10(9)/1) increased from 220 +/- 80 to 245 +/- 102 after 14 days and stabilized at that level up to day 90 (p < 0.0001). The increment was similar in complete or partial responders but was not observed in failures. Serum transferrin receptor (sTfR, a measure of total erythropoiesis) and Het rose much more progressively, but relative platelet increments correlated with relative increases in sTfR and Hct. Relative platelet increments correlated inversely with relative changes of SeFe or transferrin saturation, but not with their absolute values, nor with baseline ferritin or its progressive decrease. Although baseline platelet count was 12% higher in patients with inflammation and correlated with serum haptoglobin, relative increases were similar in patients with or without inflammation. In conclusion, rHuEpo produced a clinically minor but consistent elevation of platelet counts. These modifications were not related primarily to modifications in iron stores, functional iron deficiency, or inflammation, but paralleled the expansion of erythropoietic activity. The results suggest that rHuEpo has a small positive effect on platelet production, but it cannot be ruled out that this could be partially mediated through functional iron deficiency. [less ▲]

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See detailMeasurement of apolipoproteins B and A by radial immunodiffusion: methodological assessment and clinical applications.
Cano, M. D.; Gonzalvo, C.; Scheen, André ULg et al

in Annales de Biologie Clinique (1994), 52(9), 657-61

The clinical evaluation of apolipoproteins is of interest in order to characterize the risk profile for ischemic heart disease both in normolipidemic and hyperlipidemic subjects. In the non-specialized ... [more ▼]

The clinical evaluation of apolipoproteins is of interest in order to characterize the risk profile for ischemic heart disease both in normolipidemic and hyperlipidemic subjects. In the non-specialized and/or small practice clinical laboratory, the measurement of some apolipoproteins can be undertaken by simple methods of immunological analysis, among which radial immunodiffusion can be of interest due to its simplicity of use and because it does not require specific equipment. In this work several methodological questions concerning the measurement of plasma apolipoproteins B and A by radial immunodiffusion have been addressed; the results show that this method is particularly reliable for the apo B assay. Regression analysis between values obtained with radial immunodiffusion and radioimmunoassay was r = 0.972 for apo B and r = 0.782 for apo A. The recovery rate was above 90% for both apolipoproteins (93.8% for apo B and 99.5% for apo A). The inter and intraassay coefficients of variation were below 5%, and the detection limits were estimated as 9.6 mg/dl for apo A and 6.9 mg/dl for apo B. Neither the ingestion of a standard breakfast (500 Cal, 17 g fat, 120 mg cholesterol) 2 h prior to testing nor freezing the sample significantly affected the measurement of apolipoproteins B and A. Mean plasma concentrations of both apolipoproteins measured by radial immunodiffusion in normo and hyperlipidemic subjects are also presented. [less ▲]

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See detailModulatory Effect of Neuropeptide Y on Acetylcholine-Induced Oedema and Vasoconstriction in Isolated Perfused Lungs of Rabbit
Delaunois, Annie ULg; Gustin, Pascal ULg; Dessy-Doize, C. et al

in British Journal of Pharmacology (1994), 113(3), 973-81

1. The modulatory role of neuropeptide Y (NPY) on pulmonary oedema induced by acetylcholine and capsaicin was investigated. The effects of NPY on the haemodynamic response to acetylcholine, phenylephrine ... [more ▼]

1. The modulatory role of neuropeptide Y (NPY) on pulmonary oedema induced by acetylcholine and capsaicin was investigated. The effects of NPY on the haemodynamic response to acetylcholine, phenylephrine and substance P were also investigated. 2. Isolated, ventilated, exsanguinated lungs of the rabbit were perfused with a constant flow of recirculating blood-free perfusate. The double/arterial/venous occlusion method was used to partition the total pressure gradient (delta Pt) into four components: the arterial gradient (delta Pa), the pre- and post-capillary gradients (respectively delta Pa' and delta Pv') and the venous pressure gradient (delta Pv). Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). 3. Acetylcholine (10(-8) M to 10(-4) M) and substance P (SP, 10(-10) M to 10(-6) M) induced a concentration-dependent increase in the Kf,c. Capsaicin (10(-4) M) and 5-hydroxytryptamine (5-HT) (10(-4) M) also increased this parameter. NPY (10(-8) M) completely inhibited the effects of acetylcholine and capsaicin on the Kf,c, without preventing the effects of substance P and 5-HT. 4. Acetylcholine induced concentration-dependent vasoconstriction in the precapillary segment. The effect was inhibited by NPY and aspirin, an inhibitor of cyclo-oxygenase, while ketanserin, a 5-HT2 receptor antagonist, and SR140333, a new NK1 antagonist, had no protective effect. Phenylephrine increased delta Pa at high concentration, an effect also inhibited by NPY and aspirin. Substance P had no significant haemodynamic effect. When injected together with NPY, substance P (10(-6) M) induced a significant increase in the total pressure gradient.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailHématome extradural cervical spontané de la femme enceinte
Mahieu, X.; Kridelka, Frédéric ULg; Pintiaux, Axelle ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1994), 23(1), 99-102

Spontaneous spinal extradural hematomas are rare entities, especially during pregnancy. The authors report a case of a 26 years old women who developed as Brown-Sequard syndrome during pregnancy. The ... [more ▼]

Spontaneous spinal extradural hematomas are rare entities, especially during pregnancy. The authors report a case of a 26 years old women who developed as Brown-Sequard syndrome during pregnancy. The patient underwent first a caesarean and thereafter a prompt spinal surgical decompression. Complete sensory-motor recovery occurred after 48 hours. Differential diagnosis of cord compression, etiology of bleeding, conditions of recovery after surgery are discussed. [less ▲]

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See detailEst-ce ainsi que les jeunes Belges lisent? Résultat d'une enquête internationale
Lafontaine, Dominique ULg; Lafontaine, Annette ULg

in Enjeux : Revue de Formation Continuée et de Didactique du Français (1994), 31(7), 31

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See detailL'identité wallonne saisie par l'enquête : une approche constructiviste de l'identité collective
Jacquemain, Marc ULg; Vandekeere, Michel; Doutrelepont, René

in Res Publica (1994), 26

The paper shows how a constructivist approach of collective identity makes sense of the use of survey data to approach regional walloon identity

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See detailLes indices de déformation plio-quaternaire de la bordure occidentale du bassin des Iullemenden dans la région de Niamey (Niger).
Vicat, Jean-Paul; Léger, Jean-Marc; AHMED, Y et al

in Revue de Géographie Alpine (1994), 5

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See detailCrystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide
Merritt, E. A.; Sarfaty, S.; van den Akker, F. et al

in Protein Science : A Publication of the Protein Society (1994), 3(2), 166-75

Cholera toxin (CT) is an AB5 hexameric protein responsible for the symptoms produced by Vibrio cholerae infection. In the first step of cell intoxication, the B-pentamer of the toxin binds specifically to ... [more ▼]

Cholera toxin (CT) is an AB5 hexameric protein responsible for the symptoms produced by Vibrio cholerae infection. In the first step of cell intoxication, the B-pentamer of the toxin binds specifically to the branched pentasaccharide moiety of ganglioside GM1 on the surface of target human intestinal epithelial cells. We present here the crystal structure of the cholera toxin B-pentamer complexed with the GM1 pentasaccharide. Each receptor binding site on the toxin is found to lie primarily within a single B-subunit, with a single solvent-mediated hydrogen bond from residue Gly 33 of an adjacent subunit. The large majority of interactions between the receptor and the toxin involve the 2 terminal sugars of GM1, galactose and sialic acid, with a smaller contribution from the N-acetyl galactosamine residue. The binding of GM1 to cholera toxin thus resembles a 2-fingered grip: the Gal(beta 1-3)GalNAc moiety representing the "forefinger" and the sialic acid representing the "thumb." The residues forming the binding site are conserved between cholera toxin and the homologous heat-labile enterotoxin from Escherichia coli, with the sole exception of His 13. Some reported differences in the binding affinity of the 2 toxins for gangliosides other than GM1 may be rationalized by sequence differences at this residue. The CTB5:GM1 pentasaccharide complex described here provides a detailed view of a protein:ganglioside specific binding interaction, and as such is of interest not only for understanding cholera pathogenesis and for the design of drugs and development of vaccines but also for modeling other protein:ganglioside interactions such as those involved in GM1-mediated signal transduction. [less ▲]

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See detailLe tamoxifene dans le traitement du cancer du sein
Charlier, Corinne ULg; Colin, Claude ULg; Merville, Marie-Paule ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1994), 23(7), 751-6

Tamoxifen is the most often prescribed non steroidal antioestrogenic agent in the world for breast cancer. Worldwide collaboration. has centralized the results, of different trials throughout the world on ... [more ▼]

Tamoxifen is the most often prescribed non steroidal antioestrogenic agent in the world for breast cancer. Worldwide collaboration. has centralized the results, of different trials throughout the world on oral adjuvant therapy in the early stages of breast cancer. A significative regression of the tumour was observed in most cases. Moreover, recent epidemiological studies suggest that tamoxifen could prevent new contralateral primary tumours. The risk of the disease should thus be reduced by the prophylactic use of antioestrogens such as tamoxifen. Investigations using a variety of models have evaluated the effect of tamoxifen on tumour promotion and cell growth. Tamoxifen-induced growth inhibition is associated with major changes in biochemical events in cultured human breast cancer cells including cell proliferation or growth factor production. Growth inhibition of oestrogen-responsive human breast cancer cells is associated with an induced secretion of autoinhibitory polypeptides (TGF beta) and an antagonistic effect on the synthesis of proliferative proteins (TGF alpha,...). The first step in the mechanism of action of the drug is binding of tamoxifen to the oestrogen receptors. Development of resistance to tamoxifen treatment is a great problem in treatment of breast cancer patients and the mechanism of resistance will require further study: under the influence of the drug, tumours could become remodelled as selected subpopulations emerge resistant-tamoxifen. The fact that some breast cancers which are oestrogen receptor-negative respond to antioestrogen suggests that parallel but separate pathways for oestrogen and antioestrogen action may exist. This paper summarizes the results of the most recent studies concerning this promising drug. [less ▲]

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See detailThe experience of the CHU Liege with conservative surgery in the management of upper urinary tract tumors.
Bouffioux, Christian ULg; Andrianne, Robert ULg; Bonnet, Pierre ULg et al

in Acta Urologica Belgica (1994), 62(4), 39-43

A review of 20 cases of conservative surgery for urothelial tumors of the upper tract is presented. The morbidity is limited; the recurrence rate is around 15% in the ipsilateral urinary tract with a mean ... [more ▼]

A review of 20 cases of conservative surgery for urothelial tumors of the upper tract is presented. The morbidity is limited; the recurrence rate is around 15% in the ipsilateral urinary tract with a mean follow up of 41 months. Conservative surgery does not seem to carry a higher fatal risk as compared to more radical surgery. It has indications in some cases which are defined. [less ▲]

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See detailFate of unstimulated Latissimus Dorsi transposed into the chest and applied to cardiomyoplasty
Radermecker, M. A.; Reznik, M.; Vivario, M. et al

in Research in Surgery (1994), 6(1), 35-39

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See detailTamoxifen restores the E-cadherin function in human breast cancer MCF-7/6 cells and suppresses their invasive phenotype
Bracke, M. E.; Charlier, Corinne ULg; Bruyneel, E. A. et al

in Cancer Research (1994), 54

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See detailRock progressif et analyse musicale
Pirenne, Christophe ULg

in Art&Fact (1994), 13

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See detailLe projet d'entreprise d'insertion: son impact en termes budgétaires et d'emplois
Defourny, Jacques ULg; Lequet, Nathalie; Tilkin, Michel

in Reflets et Perspectives de la Vie Economique (1994), XXIII(1-2), 157-171

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See detailTEMPERATURE-DEPENDENCE OF GROWTH, ENZYME-SECRETION AND ACTIVITY OF PSYCHROPHILIC ANTARCTIC BACTERIA
Feller, Georges ULg; Narinx, E.; Arpigny, J. L. et al

in Applied Microbiology & Biotechnology (1994), 41(4), 477-479

Five psychrophilic Antarctic bacteria have been selected for their capacity to secrete exoenzymes into culture medium. These strains are able to grow from 0 to about 25 degrees C. However, production of ... [more ▼]

Five psychrophilic Antarctic bacteria have been selected for their capacity to secrete exoenzymes into culture medium. These strains are able to grow from 0 to about 25 degrees C. However, production of lipase from Moraxella, alpha-amylase from Alteromonas haloplanctis, beta-lactamase from Psychrobacter immobilis and protease from Bacillus is maximal at temperatures close to that of their environment (-2 to 4 degrees C) and is strongly inhibited at higher temperatures. This thermal effect involves alterations in the secretory pathway in the upper range of temperatures, losses due to the enzyme thermal lability and in some cases to reduction in cell development. The apparent optimal activity temperature of these enzymes is between 30 and 40 degrees C, i.e. about 20 degrees C lower than that of their mesophilic counterparts. [less ▲]

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See detailFirst-principles calculations of dielectric and effective charge tensors in Barium Titanate
Ghosez, Philippe ULg; Gonze, X.; Michenaud, J. P.

in Ferroelectrics (1994), 153

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See detailCritical appraisal of pressure-frequency relation for estimation of diaphragm function in intact and conscious animals
Desmecht, Daniel ULg; Linden, Annick ULg; Lekeux, Pierre ULg

in American Journal of Veterinary Research (1994), 55(12), 1638-1646

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See detailDirect n.m.r. evidence for substrate-induced conformational changes in a beta-lactamase.
Jamin, M.; Damblon, Christian ULg; Bauduin-Misselyn, A. M. et al

in Biochemical Journal (1994), 301 ( Pt 1)

Cefoxitin and other beta-lactam antibiotics with a methoxy group on the alpha-face behave as very poor substrates of the Bacillus licheniformis beta-lactamase. The kinetic properties of the enzyme ... [more ▼]

Cefoxitin and other beta-lactam antibiotics with a methoxy group on the alpha-face behave as very poor substrates of the Bacillus licheniformis beta-lactamase. The kinetic properties of the enzyme-cefoxitin system made it theoretically suitable for a detailed structural study of the acyl-enzyme. Unfortunately, soaking the crystals in cefoxitin solution did not allow detection of a crystalline acyl-enzyme complex. In contrast, direct observation by n.m.r. of the stable acyl-enzyme formed with cefoxitin and moxalactam indicated clear modifications of the enzyme structure, which were reflected in the aromatic and high-field methyl regions of the spectrum. The return to the initial free enzyme spectrum was concomitant with the hydrolysis of the acyl-enzyme, the process being slow enough to allow multidimensional n.m.r. experiments. [less ▲]

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See detailLaparoscopic adrenalectomy in pheochromocytoma and Cushing's syndrome. Reflections about two case reports.
Meurisse, Michel ULg; Joris, Jean ULg; Hamoir, Etienne ULg et al

in Acta Chirurgica Belgica (1994), 94(6), 301-6

Laparoscopic adrenalectomy is possible as well on the left as on the right side using a percutaneous transabdominal approach. The exposure of the glands seems better than it could be achieved with an open ... [more ▼]

Laparoscopic adrenalectomy is possible as well on the left as on the right side using a percutaneous transabdominal approach. The exposure of the glands seems better than it could be achieved with an open method. In combination with intraoperative infusion of nicardipine, a calcium-channel blocker, the laparoscopic removal of a pheochromocytoma was performed safely and under stable conditions. In case of Cushing's syndrome, the laparoscopic approach reduces the problems related to poor healing. In all cases of laparoscopic adrenalectomy, this approach could offer the clear advantages of smaller incisions, reduced postoperative pain and incisional discomfort as well as complications related to large and invasive procedure and finally allows quicker recovery. Moreover, conversion to open surgery remains always possible, if needed. [less ▲]

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See detailMeningoradiculoneuritis due to acyclovir-resistant varicella zoster virus in an acquired immune deficiency syndrome patient
Snoeck, R.; Gérard, M.; Sadzot-Delvaux, Catherine ULg et al

in Journal of Medical Virology (1994), 42(4), 338-347

Varicella zoster virus (VZV) is recognized as one of the major viral pathogens reactivated in patients with the acquired immune deficiency syndrome (AIDS). We report the case of meningoradiculoneuritis in ... [more ▼]

Varicella zoster virus (VZV) is recognized as one of the major viral pathogens reactivated in patients with the acquired immune deficiency syndrome (AIDS). We report the case of meningoradiculoneuritis in an AIDS patient, associated with the isolation in the cerebrospinal fluid (CSF) of a thymidine kinase (TK)-deficient, acyclovir (ACV)-resistant strain of VZV. Although the virus was sensitive in vitro to phosphonoformate (PFA), the patient did not improve during PFA therapy and finally died. Several VZV strains isolated from this patient (including two isolates from the patient's CSF) were analyzed for their TK activity and subsequently the viral TK gene was sequenced showing a major deletion leading to a truncated protein. Their susceptibility to several antiviral agents including ACV, PFA, (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), 9-beta-D-arabinofuranosyladenine (vidarabine), (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine (HPMPC), and (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) was evaluated. All the virus strains isolated from this patient remained sensitive to HPMPA and HPMPC, pointing to the potential usefulness of these acyclic nucleoside phosphonates for the treatment of ACV-resistant VZV infections in immunocompromised patients. (C) 1994 Wiley-Liss, Inc. [less ▲]

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See detailHaemodynamic dysfunctions involved in experimental bovine pneumonic pasteurellosis
Desmecht, Daniel ULg; Amory, Hélène ULg; Linden, Annick ULg et al

in Journal of Experimental Animal Science (1994), 37

The present experiments were designed Co challenge, without the bias of anaesthesia, the previously reported statement that intratracheal administration of Pasteurella haemolvtica does not impair ... [more ▼]

The present experiments were designed Co challenge, without the bias of anaesthesia, the previously reported statement that intratracheal administration of Pasteurella haemolvtica does not impair haemodynamics in calves. Central venous, pulmonary arterial and capillary wedge and systemic arterial pressures were measured by fluid-filled catheters in 11 calves. Cardiac output was measured by the thermodilution technique, heart rate (HR) was derived from the ECG and stroke volume (SV), pulmonary and systemic vascular resistance were calculated. The haemodynamic values were measured hourly from the first to the tenth hour alter intratracheal inoculation of P. haemolytica. Cardiovascular response was marked and typically consisted of two hypotensive hypodynamic phases. The first, which occurred by the second hour after inoculation, consisted of a transient bradycardia and a systemic vasodilation leading to profound hypotension and reduced venous return. Cardiac performance then recovered, but systemic hypotension persisted and hypoxaemia developed. The second phase occurred by the seventh hour alter inoculation and was associated with a decline in SV, an increase in HR, and pulmonary hypertension. Three different degrees of cardiovascular dysfunctions were found, differing basically in the intensity of the first phase bradycardia and second phase reduction in SV and systemic arterial pressure, suggesting that experimental pneumonia produced by intratracheal instillation of P. haemolytica in calves results in distinct cardiovascular dysfunctions depending on the animal. Ignorance of the underlying haemodynamic status therefore precludes reliable interpretation of physiological as well as pharmacological results using this model of respiratory disease [less ▲]

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See detailNatural-Convection Above Line Heat-Sources In Greenhouse Canopies
Aubinet, Marc ULg; Deltour, Jules

in International Journal of Heat and Mass Transfer (1994), 37(12),

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See detailAcute ethanol increases taurine but neither glutamate nor GABA in the nucleus accumbens of male rats: a microdialysis study.
Dahchour, Abdelkhader; Quertemont, Etienne ULg; De Witte, Philippe

in Alcohol & Alcoholism (1994), 29(5), 485-7

The effects of acute intraperitoneal administration of ethanol (1-3 g/kg body wt) on the extracellular concentrations of glutamate (GLU), taurine (TAU) and gamma-aminobutyric acid (GABA) in rat brain ... [more ▼]

The effects of acute intraperitoneal administration of ethanol (1-3 g/kg body wt) on the extracellular concentrations of glutamate (GLU), taurine (TAU) and gamma-aminobutyric acid (GABA) in rat brain nucleus accumbens were studied by the microdialysis technique coupled to HPLC-EC detection. Levels of GLU and GABA were not affected by ethanol, whereas those of TAU were increased by doses of the drug of 2-3 g/kg. [less ▲]

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See detailA TEF-1 binding motif that interacts with a placental protein is important for the transcriptional activity of the hCS-B enhancer
Jacquemin, P.; Oury, Cécile ULg; Belayew, A. et al

in DNA & Cell Biology (1994), 13(10), 1037-45

The transcriptional activity of the human placental lactogen genes (choriosomatomammotropic hormone, hCS) is controlled by tissue-specific enhancers located 4 kb downstream from their respective origins ... [more ▼]

The transcriptional activity of the human placental lactogen genes (choriosomatomammotropic hormone, hCS) is controlled by tissue-specific enhancers located 4 kb downstream from their respective origins of transcription. The hCS-B enhancer is the strongest; its activity is mediated by synergism between two protein-binding sites (DF-3 and DF-4). The DF-4 site possesses a potential binding sequence for TEF-1, a known transcription factor. In this paper, we show by electrophoretic mobility-shift assays and antibody supershift experiments that TEF-1 does not bind to site DF-4. Mutations in the TEF-1-like binding motif of site DF-4 prevent formation of the DNA-protein complex, called complex f, in the presence of placental JEG-3 cell extracts. When HeLa cell extracts are used, another complex (complex c) is also affected. In transient expression experiments, TKCAT constructs linked to this mutated DF-4 site exhibit greatly reduced transcriptional activity when introduced into JEG-3 cells. Some cell lines contain both protein c and protein f (the proteins forming complexes c and f); when transfected, these lines display reduced DF-4-driven activity, suggesting that the two proteins could compete for the same DF-4 sequence. We conclude that protein f is important for the placenta-specific activity of the hCS-B enhancer. By UV cross-linking, we show that protein f is actually three polypeptides ranging in size from about 12 to 21 kD. [less ▲]

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See detailSynthesis by sol-gel process and characterization of barium-titanate
LECLOUX, A. J.; KOCKLENBERG, Régine; MICHAUX, Bernard et al

in Annales de Chimie Science des Materiaux (1994), 19(2), 67-78

Barium titanate gels are prepared by sol-gel process from titanium isopropoxide and barium hydroxide in presence of methoxyethanol, methanol and water. These gels are dried under vacuum. The influence of ... [more ▼]

Barium titanate gels are prepared by sol-gel process from titanium isopropoxide and barium hydroxide in presence of methoxyethanol, methanol and water. These gels are dried under vacuum. The influence of the hydrolysis ratio, the temperature and the vacuum level during drying is discussed. [less ▲]

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See detailT-CELLS SKIN LYMPHOMA IN 3 DOGS
Gilbert, S.; Clercx, Cécile ULg; Jauniaux, Thierry ULg et al

in Annales de Médecine Vétérinaire (1994), 138(8), 587-595

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See detailGestion d'un épisode de peste porcine classique en Belgique
Vanthemsche, P.; Saegerman, Claude ULg

in Epidémiologie et Santé Animale (1994), 26

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See detailPerformances physiques et motrices d’enfants de l’enseignement fondamental engagés ou non dans un programme d’activités physiques quotidiennes
Cloes, Marc ULg; Delfosse, Catherine; Ledent, Maryse et al

in Revue de l'Education Physique (1994), 34(2-3), 59-68

La recherche d'un développement physique et moteur harmonieux et complet figure parmi les objectifs des différentes expériences proposant des activités physiques quotidiennes en milieu scolaire. La ... [more ▼]

La recherche d'un développement physique et moteur harmonieux et complet figure parmi les objectifs des différentes expériences proposant des activités physiques quotidiennes en milieu scolaire. La programmation de séances régulières d'éducation physique semble déboucher sur des niveau de condition physique supérieurs. ... [less ▲]

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See detailC'est le bouquet... Histoire d'un ornement typographique liégeois du XVIIIe siècle
Droixhe, Daniel ULg

in Gutenberg-Jahrbuch (1994), 69

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See detailUtilisation des dossiers de contenus préparés dans le cadre d’une expérience de rénovation de l’enseignement fondamental en Communauté Française de Belgique
Delfosse, Catherine; Ledent, Maryse; Cloes, Marc ULg et al

in Revue de l'Education Physique (1994), 34(2-3), 99-102

Dans toute expérience d'éducation physique quotidienne de longue durée, impliquant les instituteurs titulaires des classes, soit en responsabilité totale ou en partage de responsabilités avec le maître ... [more ▼]

Dans toute expérience d'éducation physique quotidienne de longue durée, impliquant les instituteurs titulaires des classes, soit en responsabilité totale ou en partage de responsabilités avec le maître spécial comme dans l'expérience de rénovation de l'enseignement fondamental en Communauté française de Belgique, il est primordial de fournir une aide en matière de contenus d'enseignement. Il est évident qu'un généraliste comme l'est l'instituteur ne dispose que d'un éventail relativement limité d'exercices ou de situations à proposer aux enfants. Deux types d'aide sont généralement proposées: des documents et des consultations régulières avec des spécialistes. ... [less ▲]

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See detailCharacterization of a single strong tissue-specific enhancer downstream from the three human genes encoding placental lactogen
Jacquemin, Patrick; Oury, Cécile ULg; Peers, Bernard ULg et al

in Molecular & Cellular Biology (1994), 14(1), 93-103

The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3 ... [more ▼]

The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3'. So far, a single placenta-specific enhancer has been identified in the locus, 2 kb downstream from the hCS-B gene, and shown to comprise one in vitro binding site for a nuclear protein. We here provide evidence that the hCS-B enhancer is more complex: (i) protection against DNase I digestion in the 3' flanking region of the hCS-B gene reveals four binding sites (DF-1, DF-2, DF-3, and DF-4) for nuclear proteins from either placental or HeLa cells, and (ii) placenta-specific enhancer activity can be fully exerted in transient expression experiments by a 126-bp fragment comprising the DF-3 and DF-4 protein-binding sites. By dissecting this region, we show that enhancer activity is mediated by a synergy between DF-3 and DF-4. Competitions with various oligonucleotides in footprinting and gel retardation experiments indicate that the same protein or set of proteins, different in HeLa and placenta cell nuclei, interacts with sites DF-2, DF-3, and DF-4. We also studied the regions of the hCS-L and hCS-A genes which are highly similar to the hCS-B enhancer. Although they each present the same four protein-binding sites, they exhibit only minor enhancer activity. [less ▲]

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See detailA new species of trametes from Papua New Guinea
Castillo Cabello, Gabriel ULg

in Mycotaxon (1994)

Trametes demoulinii from Papua New guinea is descrebed as new. The new taxon is closest to Trametes conchifer and Trametes membranacea, both from America and Trametes cingulata know in Africa and East ... [more ▼]

Trametes demoulinii from Papua New guinea is descrebed as new. The new taxon is closest to Trametes conchifer and Trametes membranacea, both from America and Trametes cingulata know in Africa and East Asia. In addition to basidiomata, cultural characters of the new species are described. [less ▲]

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See detailLe Christ de Wavreumont
Van den Bossche, Benoît ULg

in Folklore. Stavelot – Malmedy – Saint-Vith (1994), 55

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See detailANOMALOUS PERTURBATIVE TRANSPORT IN TOKAMAKS DUE TO DRIFT-WAVE TURBULENCE
Thoul, Anne ULg; SIMILON, P. L.; SUDAN, R. N.

in Physics of Plasmas (1994), 1(3), 579-600

A new method for calculating the anomalous transport in tokamak plasmas is presented. The renormalized nonlinear plasma response function is derived using the direct-interaction approximation (DIA). A ... [more ▼]

A new method for calculating the anomalous transport in tokamak plasmas is presented. The renormalized nonlinear plasma response function is derived using the direct-interaction approximation (DIA). A complete calculation for the case of electrostatic drift-wave turbulence is presented. Explicit expressions for all coefficients of the anomalous transport matrix relating particle and heat fluxes to density and temperature gradients in the plasma are obtained. The anomalous transport matrix calculated using the DIA does not have the Onsager symmetry. As an example of application, the parameters of the Texas Experimental Tokamak (TEXT) [Nucl. Technol. Fusion 1, 479 (1981)] are used to evaluate all transport coefficients numerically, as well as the spectrum modulation. The relation between the theoretical results and the experimental data is discussed. Although this paper focuses on electron transport for simplicity, the method can also be used to calculate anomalous transport due to ion instabilities, such as the ion-temperature-gradient instability. [less ▲]

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See detailHIGH-RESOLUTION SPECTRA OF JUPITER NORTHERN AURORAL ULTRAVIOLET EMISSION WITH THE HUBBLE-SPACE-TELESCOPE
TRAFTON, L. M.; Gérard, Jean-Claude ULg; Munhoven, Guy ULg et al

in Astrophysical Journal (1994), 421(2), 816-827

The first spectroscopic observations of planetary aurora with the HST are reported. These include spectral regions centered on the H-2 Lyman and Werner bands of a region of Jupiter's northern aurora. The ... [more ▼]

The first spectroscopic observations of planetary aurora with the HST are reported. These include spectral regions centered on the H-2 Lyman and Werner bands of a region of Jupiter's northern aurora. The observations were made with the Goddard High Resolution Spectrograph (GHRS) using the Large Science Aperture as part of a campaign to study Jupiter at the time of the Ulysses flyby. The individual rotational-vibrational bands are resolved and the observed emissions are essentially all from H-2. A rotational-vibrational temperature for H-2 of 530 +/- 100 K is derived, a value significantly less than the 850-1100 K reported for Jovian H-3(+) in the near-infrared but consistent with the temperature reported for fundamental-band quadrupole H-2 emission. Comparison with the Faint Object Camera (FOC) images shows that the observed region was not one of the hot spots of the aurora. The results are interpreted in terms of electron impact excitation of H-2 from secondary particles generated by primaries precipitating into Jupiter's atmosphere from the magnetosphere. In the region of the aurora observed, the homopause level is found to be significantly hotter but not necessarily higher than observed at nonauroral latitudes. The equatorial H-2 dayglow spectrum was also detected; its intensity was 3.2 kR or 13% of the strength of the observed auroral emission. [less ▲]

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See detailDe l'utopie en architecture. Une technique pour le Corbusier ?
Tieleman, David ULg

in Art&Fact (1994), 13

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See detailL'enseignement du français instrumental à l'université: quelles possibilités?
Vanden Berghe, Kristine ULg; Sanctobin, Véronique; Slootmaekers, An et al

in Rencontres (1994), 5

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See detailDe l'utopie en architecture
Tieleman, David ULg

in A+ (1994)

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See detailInfluence de la pluie sur l'utilisation d'un abri par des taurillons au pâturage
Vandenheede, Marc ULg; Sheri, R.; Nicks, Baudouin ULg et al

in Annales de Médecine Vétérinaire (1994), 138

The time spent under a shelter by 8 grazing fattening bulls of the Belgian Blue breed was recorded during 35 days using a time-lapse recorder with IR illumination. The observation period was divided into ... [more ▼]

The time spent under a shelter by 8 grazing fattening bulls of the Belgian Blue breed was recorded during 35 days using a time-lapse recorder with IR illumination. The observation period was divided into 166 hours with rain (20 %) and 674 hours without precipitation (80 %). An occupation rate was calculated for each hour as the sum of the time spent under the shelter by each of the 8 bulls divided by 480 (8 animals x 60 minutes). The average occupation rates were 15.4 +/- 29.1 % for the hours with rain and 4.5 +/- 14.8 % for the hours without precipitation (P<0.001). The occupation rate was significantly influenced by intensity and duration of the rain : the occupation rate being higher during rainfall than with dry weather when the intensity of the rain was at least 0.4 l/m2 and the duration more than 2 hours. There was no significant difference between the live weight gain of the animals with a shelter and of other bulls grazing on an adjacent pasture without shelter. Our observations suggested however that a shelter may improve the welfare of the animals. [less ▲]

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See detailCaracteristiques des phosphatases acides de l'orge en condition de stress hydrique.
Ben-Naceur, M'B.; Paul, Roger ULg; Portetelle, Daniel ULg et al

in Cahiers Agricultures (1994), 3(4),

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See detailMarqueurs tumoraux
Charlier, Corinne ULg; Plomteux, Guy ULg

in Focus Diagnostica (1994), 2

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See detailCardio-respiratory, haematological and biochemical parameter adjustments to exercise: effect of a probiotic in horses during training
Art, Tatiana ULg; Votion, Dominique ULg; Mc Entee, Kathleen ULg et al

in Veterinary Research (1994), 25(4), 361-370

Two randomly distributed groups of thoroughbred horses were compared during a 12-week period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detraining. The ... [more ▼]

Two randomly distributed groups of thoroughbred horses were compared during a 12-week period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detraining. The horses were trained following the same standardized schedule and were regularly investigated using standardized treadmill exercise tests (SET) of increasing speed. After the first SET and during the whole experimental period, a group of 6 horses received a probiotic (Bioracing) once a day while a group of 5 horses received a placebo. All other conditions were similar for both groups. During each SET, the oxygen uptake, carbon dioxide output, tidal volume (inspired volume), respiratory rate and expired minute volume were obtained using 2 ultrasonic pneumotachographs and a mass spectrometer. All the parameters were the mean of the values calculated during the last 20 s of the SET. Heart rate was continuously measured with a polar horse tester. Venous blood was sampled before and after the test and analyzed for various biochemical parameters. In both groups, training induced significant modification in most of the cardio-respiratory parameters, ie peak oxygen uptake, peak carbon dioxide output, respiratory exchange ratio, ventilation/min to oxygen-uptake ratio and oxygen-uptake to heart-rate ratio. After the 3-week detraining period, most of the values were again similar to the pre-training values in both groups. However, the training-induced modifications of most of the cardio-respiratory parameters occurred earlier and were proportionally greater in the probiotic-treated group than in the control. The respiratory coefficient decreased in the control but not in the treated group. All other parameters changed similarly in both groups. This suggests that Bioracing could modify the physiological effects of training by improving some aerobic metabolic capacities for carbohydrate utilization, but that this effect occurs only during training and not during periods of physical inactivity [less ▲]

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See detailLe diabète insipide central : l'étiologie n'est pas toujours évidente
Geenen, Vincent ULg

in Actualité Médicale Belge (1994), 444

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See detailEvaluation of agents used in osteoporosis: What are the needs ?
Reginster, Jean-Yves ULg

in Osteoporosis Japan (1994), 2

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See detailMeasurement of Right Ventricular Ejection Fraction and Volume by the Thermodilution Technique in the Unsedated Calf
Amory, Hélène ULg; Mc Entee, Kathleen ULg; Linden, Annick ULg et al

in Research in Veterinary Science (1994), 56(1), 75-81

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end ... [more ▼]

This study was designed to test the applicability and the reproducibility of the thermodilution method in the measurement of right ventricular ejection fraction (EF), end-diastolic volume (EDV) and end-systolic volume (ESV) in unsedated cattle. In the 16 healthy calves studied, it was possible, using a fast response thermodilution catheter, to obtain characteristic exponential steplike washout curves, allowing the calculation of right ventricular EF, EDV and ESV. Five to 10 successive thermodilution curves were recorded within a few minutes and two to five similar sets of measurements were performed throughout the same day to test the reproducibility of the technique. The same protocol was repeated the following day. Right ventricular EF, EDV and ESV were reproducible successively, throughout a given day and on following days. The mean intraset, intraday and interdays coefficients of variation ranged from a mean of 4.0 (SD 4.1) per cent to a mean of 18.2 (SD 7.9) per cent. Values of right ventricular volumes and EF agreed with those previously reported in various animal species. Thermodilution was, therefore, shown to be a useful method for measuring with a satisfactory reproducibility, right ventricular EF, EDV and ESV in the unsedated healthy calf [less ▲]

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See detailComparaison pratique de quelques méthodes d'analyse discriminante
Lazaar, N.; Palm, Rodolphe ULg

in Biométrie-Praximétrie (1994), 34

Dans cette étude, on compare les performances des trois méthodes d'analyse discriminante suivantes : analyse discriminante linéaire, analyse discriminante quadratique et analyse discriminante logistique ... [more ▼]

Dans cette étude, on compare les performances des trois méthodes d'analyse discriminante suivantes : analyse discriminante linéaire, analyse discriminante quadratique et analyse discriminante logistique. Les performances sont étudiées dans le cas de données réelles, en envisageant diverses transformations de variables destinées à modifier les conditions de normalité et d'égalité des variances entre populations. Aucune différence significative de performances entre méthodes n'a pu être mise en évidence au niveau de signification de 0,05. Par contre, les performances des trois méthodes diminuent quand les distributions des variables sont nettement non normales ou de variances très inégales. Différentes estimations des taux d'erreur ont également été comparées : resubstitutin, extraction-insertion et utilisation d'un échantillon-test. Les résultats ont montré que, par rapport à l'utilisation d'échantillons-tests, la méthode de resubstitution sous-estime le taux d'erreur. Cette sous-estimation est de l'ordre de 13 % du taux d'erreur réel. La méthode d'extraction-insertion donne par contre des résultats très semblables à la méthode utilisant un échantillon-test. [less ▲]

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See detailIdentification Of Functional-Sites On Bovine Leukemia-Virus Envelope Glycoproteins Using Structural And Immunological Data
Callebaut, I.; Portetelle, Daniel ULg; Burny, A. et al

in European Journal of Biochemistry (1994), 222(2),

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See detailValeur semeiologique des examens de laboratoire: application multicentrique au depistage des metastases hepatiques.
Bourguignat, Alain; Albert, Adelin ULg; Dumont, Gilles et al

in Annales de Biologie Clinique (1994), 52(11), 787-97

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See detailThiamine in Excitable Tissues: Reflections on a Non-Cofactor Role
Bettendorff, Lucien ULg

in Metabolic Brain Disease (1994), 9(3), 183-209

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See detailSequencing And Analysis Of A 20.5-Kb Dna Segment Located On The Left Arm Of Yeast Chromosome-Xi
Vandenbol, Micheline ULg; Bolle, Pa.; Dion, C. et al

in Yeast (1994), 10

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See detailParadoxe sur le philosophe (kant entre Bourdieu et Derrida)
Steinmetz, Rudy ULg

in Réseaux : Revue Interdisciplinaire de Philosophie Morale et Politique (1994)

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See detailGéologie de la région de Puagne, Bande de Sambre-et-Meuse (Belgique). Hypothèses basées sur l'état de maturation des palynomorphes fossiles
Steemans, Philippe ULg

in Comptes Rendus de l'Académie des Sciences. Série 2, Sciences de la Terre et des Planètes (1994), 318(11), 1551-1556

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See detailL'analyse des glucosinolates, travaux réalisés par l'Organisation internationale de standardisation et la CEE
Wathelet, Jean-Paul ULg; Ribaillier, Daniel; Severin, Michel et al

in Bulletin du GCIRC (1994)

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See detailGender and diagnostic performance of the growth hormone response to clonidine for major depression : A large scale multicenter study
Schittecatte, Michel; Charles, Gerard; Machowski, R. et al

in American Journal of Psychiatry (The) (1994)

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See detailSequence-Analysis Of A 40.2-Kb Dna Fragment Located Near The Left Telomere Of Yeast Chromosome-X
Vandenbol, Micheline ULg; Durand, Patrick; Bolle, P. A. et al

in Yeast (1994), 10(12),

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See detailConditional watershed segmentation for grain boundary reconstruction
Pirard, Eric ULg; Delanaye, Annick ULg

in Acta Stereologica (1994), 14(1), 23-28

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See detailLes souches attachantes et effaçantes d'Escherichia coli d'origine bovine
Mainil, Jacques ULg; Pohl, P.

in Annales de Médecine Vétérinaire (1994), 138

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See detailConflits collectifs et recours juridictionnels
Kefer, Fabienne ULg

in Journal des Tribunaux (1994)

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See detailPreliminary of observations on oribatid mites of riparian soils in Uruguay
Sarasola, M.; Vera, H.; Wauthy, G. et al

in Bulletin et Annales de la Société Royale Belge d’Entomologie = Bulletin en Annalen van de Koninklijke Belgische Vereniging voor Entomologie (1994), 130

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See detailDémographie du martin pêcheur (Alcedo atthis): incidences climatiques sur le succès reproducteur
Libois, Roland ULg

in Gerfaut (Le) = Giervalk (De) (1994), 84

It is well known that the Kingfisher populations decrease dramatically after a sever winter. The recovery depends on the climatic conditions of the next reproductive seasons. In order to understand the ... [more ▼]

It is well known that the Kingfisher populations decrease dramatically after a sever winter. The recovery depends on the climatic conditions of the next reproductive seasons. In order to understand the mechanism involved in this climatic influence, the fate of more than 500 broods was studied between 1985 and 1993. The study area comprises the river Meuse from Vireux (F,08) downstreams to Huy (B Liège) and the lower part of its main tributaries. The first egg laying date was determined for each brood, eggs were counted, pulli ringed at nest and the adults controlled and ringed when possible. Our main conclusions are i : the beginning of the first clutch is correlated with the thermic conditions of March. ii : the clutches are not synchronised, even on a same watercourse. iii : the egg probuction and the hatching success don't show clear variations between years abd habitat types (river, ponds and small streams) or depending on the clutch rank. iv : the fledged young production depends on how much broods are raised by the different pairs. v : the probability a pair is attempting a thrid brood is function of how early the first clutch is layed, the age of the male (older males settle earlier and are more productive), the habitat quality and the weather conditions (rain, temperature) in May. [less ▲]

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See detailAnalysis methodology, experimental investigation and computer optimization of a passive solar commercial building in the Belgian climate
Andre, Philippe ULg; Nicolas, Jacques ULg; Rivez, Jean-François et al

in Solar Energy (1994), 52(1), 9-25

The Fondation Universitaire Luxembourgeoise (FUL) building (completed in 1986) has been selected as a Belgian candidate for participation in the IEA (International Energy Agency) Task XI project devoted ... [more ▼]

The Fondation Universitaire Luxembourgeoise (FUL) building (completed in 1986) has been selected as a Belgian candidate for participation in the IEA (International Energy Agency) Task XI project devoted to passive solar commercial buildings. Therefore, an evaluation methodology has been set up and a monitoring plan has been defined and performed. Together with this experimental investigation, intensive computer simulation work has defined an optimized design of the building. The main results show that the recorded performance can be improved to an extent of 15% energy savings, which yields a correct performance for a passive solar building in the temperate maritime Belgian climate. [less ▲]

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See detailTHERMAL-CONDUCTIVITY OF PURE OR IRON-DOPED YBA2CU3O7-DELTA WITH OR WITHOUT AN EXCESS OF CUO
Houssa, M.; Bougrine, Hassan ULg; Ausloos, Marcel ULg et al

in Journal of Physics : Condensed Matter (1994), 6(31), 6305-6316

The thermal conductivities of YBa2(Cu1-xFex)3O7-delta + y% CuO ceramics for 0 < x < 0.03 and 0 < y < 5 have been measured. The thermal conductivities of all samples exhibit a minimum in the vicinity of ... [more ▼]

The thermal conductivities of YBa2(Cu1-xFex)3O7-delta + y% CuO ceramics for 0 < x < 0.03 and 0 < y < 5 have been measured. The thermal conductivities of all samples exhibit a minimum in the vicinity of the critical temperature T(c) and a maximum near T(c)/2. These results are interpreted with the help of an electronic model, i.e. supposing that the main contribution to the thermal conductivity below T(c) is due to electron scattering in the CuO2 planes. Within a simple two-fluid model derived from kinetic theory, taking into account the temperature dependence of the electronic relaxation time and the normal charge carrier concentration, we obtain theoretical curves which reproduce the experimental results quite well. We also take into account the porosity of the samples (in term of an intergrain contribution) to derive the electronic thermal conductivity. The parameter values of the model and the observed minimum are explained in terms of physical properties. In particular, the contribution of superconductivity fluctuations to the thermal conductivity is shown to be negligible with respect to other mechanisms. [less ▲]

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See detailRelations à autrui et abus sexuel chez de jeunes délinquantes
Spitz, J.; Born, Michel ULg

in Bulletin de Criminologie (1994), 1

L'analyse des relations qu'entretiennent des adolescentes délinquantes avec leur entourage met en évidence des différences significatives selon que celles-ci ont été victimes ou non d'un abus sexuel dans ... [more ▼]

L'analyse des relations qu'entretiennent des adolescentes délinquantes avec leur entourage met en évidence des différences significatives selon que celles-ci ont été victimes ou non d'un abus sexuel dans leur enfance. Les rapports interpersonnels des adolescentes abusées se caractérisent par l'ambivalence et la dépendance, alors qu'une dimension de pouvoir et de domination détermine ceux des adolescentes non abusées. La prise en compte du niveau de maturité sociale de ces adolescentes, parallèlement à leurs modes de relations, permet la comparaison avec les types de délinquantes décrits par Margaret Warren et donne un sens particulier à leur délinquance persistante. [less ▲]

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See detailStructural Similarities Between Chaperone Molecules Of The Hsp60 And Hsp70 Families Deduced From Hydrophobic Cluster-Analysis
Callebaut, I.; Catelli, Mg.; Portetelle, Daniel ULg et al

in Febs Letters (1994), 342(3),

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See detailTechnique for Ventricular Wrapping in Experimental Dynamic Cardiomyoplasty
Radermecker, Marc ULg; Bonnet, Pierre ULg; Philippart, C. et al

in Acta Chirurgica Belgica (1994), 94(5, Sep-Oct), 266-71

Cardiomyoplasty is a wrapping of an electrostimulated latissimus dorsi muscle flap around the heart for substitution and/or reinforcement. The surgical technique for reproducible cardiomyoplasties in the ... [more ▼]

Cardiomyoplasty is a wrapping of an electrostimulated latissimus dorsi muscle flap around the heart for substitution and/or reinforcement. The surgical technique for reproducible cardiomyoplasties in the goat model is presented. The methods combine the direction of wrapping (clockwise, counter-clockwise) and the orientation of muscle fibers. A cardiomyoplasty model using right and left latissimus dorsi or left latissimus dorsi and pectoralis major as well as a split muscle technique are illustrated. [less ▲]

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See detailBI-BASED 2223 SUPERCONDUCTING POLYCRYSTALLINE MATERIALS PREPARED BY EITHER A SOLID-STATE ROUTE OR A GLASSY MATRIX PRECURSOR METHOD - CHEMICAL-ANALYSIS AS WELL AS ELECTRICAL AND THERMAL TRANSPORT-PROPERTIES
Cloots, Rudi ULg; Bougrine, Hassan ULg; HOUSSA, M. et al

in Physica C (1994), 231(3-4), 259-270

Two routes have been followed in order to prepare Bi1.7Pb0.3Sr2Ca2Cu3O(y) superconducting materials. Bi1.7Pb0.3CuO4 is always used as one of the starting basic materials. The first route involves a new ... [more ▼]

Two routes have been followed in order to prepare Bi1.7Pb0.3Sr2Ca2Cu3O(y) superconducting materials. Bi1.7Pb0.3CuO4 is always used as one of the starting basic materials. The first route involves a new experimental procedure based on the solid state synthesis of separate intermediate phases, mixed together in a two (Bi1.7Pb0.3CuO4 + 2SrCaCuO3), three (Bi1.7Pb0.3CuO4+2SrCuO2+2CaCO3) or four (Bi1.7Pb0.3CuO4+(1/12)Sr8Ca6Cu24O41 + (3/2)CaCO3 + (4/3)SrCO3) powder process. Electric resistivity versus temperature measurements are presented. Even though the T(c)(0) of each sample is above 100 K, X-ray diffraction shows that the main phase is the 2212 phase in each case. Relatively pure 2223 materials have, however, successfully been produced by the second method, which starts from a glassy intermediate phase and involves a so-called 'matrix' method. The glass precursor phase is composed of one (Bi1.7Pb0.3CuO4), two (Bi1.7Pb0.3CuO4 + 2CaCO3), and three (Bi1.7Pb0.3CuO4 + (3/2)CaCO3 + (4/3)SrCO3) components as for the solid state routes. The role of both the added alkaline earth crystalline compounds and the glass matrix precursors in this second method turns out to be very important. We propose a schematic structural model in order to explain the selectivity of the starting materials. SrCaCuO3 is found to be a good precursor of the 2212 phase, while SrCuO2 gives rise to the 2223 phase. It seems that the presence of CaCO3 in the glassy intermediate phase, thus acting as a cation reservoir, is very useful for the production of the 2223 phase. Electric resistivity, thermal conductivity and thermopower versus temperature measurements are also presented and discussed in terms of intergrowths and variations of cationic compositions in the Bi-based layers. [less ▲]

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See detailEffect of selenium compounds on murine B16 melanoma cells and pigmented cloned pB16 cells
Siwek, Brigitte; Bahbouth, E.; Serra, M. A. et al

in Archives of Toxicology (1994), 68(4), 246-254

Seleno-DL-cysteine and sodium selenate are toxic in B16 cells and this toxicity seems to involve glutathione metabolites.

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See detailLa structure de la typologie de Holland : une approche factorielle confirmatoire
Broonen, Jean-Paul ULg; El Ahmadi, A.

in Psychologie et Psychométrie (1994)

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See detailReaction Dynamics of Collisionally activated Ammonia Cations.
Leyh, Bernard ULg; Hoxha, A.

in Chemical Physics (1994), 179

In the framework of a research on the collisional mechanisms involved in tandem mass spectrometry, the collisional activation processes of NH3+ with different target gases have been investigated. The ... [more ▼]

In the framework of a research on the collisional mechanisms involved in tandem mass spectrometry, the collisional activation processes of NH3+ with different target gases have been investigated. The experiments have been performed on an upgraded AEI-MS9 sector instrument equipped with two collision cells and include daughter ion spectra, ion kinetic energy spectra, determination of kinetic energy release distributions (KERD) and appearance energy measurements. We have shown that collisionally activated dissociation (CAD) of NH3+ into NH++[H,H] depends upon the internal energy of NH3+. This internal energy effect is also observed for the NH2++H channel, when Xe is the target gas; with He, such an effect could not be detected. The analysis of the KERD shows that production of NH2+ upon CAD takes place from the NH3+ ground electronic state by rotational predissociation; two components are observed, one which corresponds to a near-threshold process (mean kinetic energy release <T>=0.13 eV), and one characterized by <T>=0.56 ev corresponding to an excess energy of 2.1 eV above the dissociation threshold. NH+ fragments can be formed by two processes: the first one occurs at threshold and leads to NH+(X2Pi)+H2(X1Sigma+g); the second one is characterized by large KER values (up to 7 eV) and leads to either electronically excited NH+ or dissociation of H2. [less ▲]

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See detailCationic and secretory effects of BPDZ 44 and diazoxide in rat pancreatic islets
Antoine, M.-H.; Pirotte, Bernard ULg; Hermann, M. et al

in Experientia (1994), 50

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See detailTopographic forcing of the circulation of LIW in the Western Mediterranean
Alvarez, A.; Tintoré, J.; Holloway, G. et al

in Coastal Estuarine Studies (1994), 46

Circulation of the Western Mediterranean has been inferred from tracer properties and is the object of numerical modelling studies (eg., Beckers, 1991). The path of the Levantine Intermediate Water (LIW ... [more ▼]

Circulation of the Western Mediterranean has been inferred from tracer properties and is the object of numerical modelling studies (eg., Beckers, 1991). The path of the Levantine Intermediate Water (LIW), thought o be a buoyancy forced flow, provides an indicator of mid-depth circulation (Millot, 1987b). However, modelling studies which include buoyancy forcing appear to misrepresent the flow of LIW. We have used the 'GFDL Modular Ocean Model' (MOM) to explore the suggestion of Holloway (1992) that subgridscale eddy-topography interaction may be responsible for such systematic defects. We have executed MOM with and without the parametrization of the eddytopography interaction. The different results are compared with circulation inferred from observational data. [less ▲]

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See detailNote sous l'arrêt de la CJCE du 19 janvier 1994, Eurocontrol
Partsch, Philippe-Emmanuel ULg

in Journal des Tribunaux Droit Européen (1994)

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See detailEtude du reservoir genetique de la legumineuse alimentaire Phaseolus lunatus L. par l'analyse electrophoretique d'isozymes.
Maquet, A.; Wathelet, Bernard ULg; Baudoin, Jean-Pierre ULg

in Bulletin des Recherches Agronomiques de Gembloux (1994), 29(3),

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See detailMétaux lourds et isotopes stables du carbone dans la matière particulaire: distribution sur le plateau continental nord-ouest européen
Dauby, Patrick ULg; Mosora, Fiorentina; Frankignoulle, Michel et al

in Bulletin de la Société Royale des Sciences de Liège (1994), 63

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See detailQuantitative and qualitative study of the production of broad-spectrum antifungal lipopeptides from Bacillus subtilis S499.
Jacques, Ph.; Hbid, Ch.; Vanhentenryck, F. et al

in Progress in biotechnology (1994), 9

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See detailMadin Darby Bovine Kidney Cell Synchronization by Lovastatin: Application to Bovine Herpesvirus-1 Gene Expression
Vanderplasschen, Alain ULg; Hanon, E.; Benarafa, C. et al

in Veterinary Research (1994), 25(6), 555-67

The number of investigations involving cell proliferation has increased rapidly in the last years. One of the major difficulties in studying cell-cycle-related events is obtaining highly synchronous cell ... [more ▼]

The number of investigations involving cell proliferation has increased rapidly in the last years. One of the major difficulties in studying cell-cycle-related events is obtaining highly synchronous cell populations without metabolic imbalance. This study demonstrates that the Madin Darby bovine kidney (MDBK) cells, a commonly used cell line in veterinary research, can be effectively synchronized using lovastatin (Lov), a drug used to treat hypercholesteremia in humans. This was demonstrated by the following results: (i) Lov inhibits cell proliferation in a dose-dependent manner; (ii) Lov synchronizes MDBK cells mainly in the G1 and secondarily in the G2+M cell-cycle phases; (iii) the cytostatic effect of Lov can be specifically inhibited by addition of mevalonate (Mev) (Lov inhibits the synthesis of Mev); (iv) removal of Lov from G1-arrested cultures, followed by addition of Mev, resulted in the synchronous recovery of DNA synthesis; and (v) 5-bromo2'-deoxyuridine incorporation experiments revealed that MDBK cells synchronization by Lov can be followed for at least 3 cycles after removal of Lov and addition of Mev. Furthermore, as an application of investigations based on the availability of synchronized MDBK, we showed that bovine herpesvirus-1 gene expression is independent on the cell cycle. [less ▲]

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See detailTests standardisés de routine pour l'évaluation de la condition physique chez les chevaux d'endurance
Noel de Burlin, A; Art, Tatiana ULg; Amory, Hélène ULg et al

in Pratique Vétérinaire Equine (1994), 26

The purpose of this study was to try to assess the fitness of endurance horses by using a simple routine standardized treadmill exercise test performed before and after a training period. Seven endurance ... [more ▼]

The purpose of this study was to try to assess the fitness of endurance horses by using a simple routine standardized treadmill exercise test performed before and after a training period. Seven endurance horses aged 7 to 13 years, unfamiliar with the treadmill conditions and competing in international championship were tested twice in an air-conditioned laboratory, i.e. before and after a 4-month training period. The test was preceded by a 20 min warm-7 period and consisted of 2 min walk and 2 min trot (4.2 m/sec) with a slope of 0, 2, 4, 6, 8 and 10 The whole test lasted 45 min. Venous blood was sampled before and 4 min after each test. Heart rate was continuously recorded before, during and after the test with a cardiometer. Rectal temperature, respiratory rate and body weight were measured before and after the test. The completion of the test induced a significant decrease of the body weight, a significant increase in packed cell volume, cortisol, white blood cells, proportion of granulocytes, lactate, free fat acids and iso-enzyme LDH 5, and no change in the ions and in the activity of the other enzymes. Training did not induce significant changes in blood parameters, except in hacked cell volume and cortisol. Training induced a significant decrease in heart rate at all exercise intensities and during recovery. It was concluded that the test proposed in this study is simple, safe, suitable for horses unfamiliar with the treadmill and applicable in a 1-day clinic routine examination. In these conditions, the most reliable parameter for the assessment of fitness in endurance horses seems to be the heart rate recorded during 2 standardized tests performed before and after a training period [less ▲]

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See detailLigamentoplasties du genou: l'état musculaire préopératoire conditionne t il le degré d'amyotrophie postopératoire?
Croisier, Jean-Louis ULg; Delcour, J. P.; Crielaard, Jean-Michel ULg

in Annales de Réadaptation et de Médecine Physique (1994), 37

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See detailAnsiedad y rendimiento academico
Egido, A.; Born, Michel ULg; Sineiro, Garcia

in Revista Galega de Psicopedagogia (1994), 9(6), 157-168

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See detailPhylogenetic relationships among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis
Nelissen, Bart; Wilmotte, Annick ULg; Neefs, Jean-Marc et al

in Systematic & Applied Microbiology (1994), 17

The cyanobacterial genera Spirulina and Arthrospira are both characterized by helical trichomes but their taxonomy is controversial and a phylogenetic study is necessary. The sequence of the 16S rRNA gene ... [more ▼]

The cyanobacterial genera Spirulina and Arthrospira are both characterized by helical trichomes but their taxonomy is controversial and a phylogenetic study is necessary. The sequence of the 16S rRNA gene and the spacer between the 16S and 23S rRNA genes (ITS) was determined for three filamentous coiled cyanobacteria: Spirulina PCC 6313, Arthrospira PCC 7345 and Arthrospira PCC 8005. A distance tree based on the 16S rRNA sequences was constructed using the neighbor-joining method. This tree shows that the two Arthrospira strains are not closely related to the Spirulina strain but belong to a cluster of strains assigned to the genera Oscillatoria, Lyngbya, and Microcoleus. The strain Spirulina PCC 6313 belongs to a branching containing unicellular cyanobacteria. For the two Arthrospira strains, the sequenced ITS region contains the tRNA(Ile) and tRNA(Ala) genes, whereas the spacer region of strain Spirulina PCC 6313 contains only the tRNA(Ile) gene. [less ▲]

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See detailLe plan régional d'aménagement du territoire wallon
Delnoy, Michel ULg; Pâques, Michel ULg

in Aménagement - Environnement (1994), n° spécial

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See detailTypage moléculaire de Clostridium perfringers.
Daube, Georges ULg; Simon, P.; Limbourg, B. et al

in Annales de Médecine Vétérinaire (1994), 138

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See detailThe Compartmentation of Phosphorylated Thiamine Derivatives in Cultured Neuroblastoma Cells
Bettendorff, Lucien ULg

in Biochimica et Biophysica Acta (1994), 1222(1), 7-14

Thiamine transport in cultured neuroblastoma cells is mediated by a high-affinity carrier (KM = 40 nM). In contrast, the uptake of the more hydrophobic sulbutiamine (isobutyrylthiamine disulfide) is ... [more ▼]

Thiamine transport in cultured neuroblastoma cells is mediated by a high-affinity carrier (KM = 40 nM). In contrast, the uptake of the more hydrophobic sulbutiamine (isobutyrylthiamine disulfide) is unsaturable and its initial transport rate is 20-times faster than for thiamine. In the cytoplasm, sulbutiamine is rapidly hydrolyzed and reduced to free thiamine, the overall process resulting in a rapid and concentrative thiamine accumulation. Incorporation of radioactivity from [14C]thiamine or [14C]sulbutiamine into intracellular thiamine diphosphate is slow in both cases. Despite the fact that the diphosphate is probably the direct precursor for both thiamine monophosphate and triphosphate, the specific radioactivity increased much faster for the latter two compounds than for thiamine diphosphate. This suggests the existence of two pools of thiamine diphosphate, the larger one having a very slow turnover (about 17 h); a much smaller, rapidly turning over pool would be the precursor of thiamine mono- and triphosphate. The turnover time for thiamine triphosphate could be estimated to be 1-2 h. When preloading the cells with [14C]sulbutiamine was followed by a chase with the same concentration of the unlabeled compound, the specific radioactivities of thiamine and thiamine monophosphate decreased exponentially as expected, but labeling of the diphosphate continued to increase slowly. Specific radioactivity of thiamine triphosphate increased first, but after 30 min it began to slowly decrease. These results show for the first time the existence of distinct thiamine diphosphate pools in the same homogeneous cell population. They also suggest a complex compartmentation of thiamine metabolism. [less ▲]

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See detailComparaison de plusieurs types d'unités d'échantillonnage dans la perspective d'un inventaire forestier régional.
Lecomte, H.; Hebert, Jacques ULg; Rondeux, Jacques ULg

in Forestry Chronicle (1994), 70(3), 304-310

This study has been conducted in order to define the guidelines of a permanent forest inventory methodology that could applied to heterogenous forests located in the southern part of Belgium. Different ... [more ▼]

This study has been conducted in order to define the guidelines of a permanent forest inventory methodology that could applied to heterogenous forests located in the southern part of Belgium. Different types of sampling units generally used in European national inventories have been compared: fixed area sampling units (based upon 3 concentric circles), non fixed-area sampling units (Bitterlich plots using basal area factor of 2 and 4) and sampling units based upon a minimum number of trees (including nearly 15 measurable trees). In true conditions of application 259 sampling points have been selected according to a systematic rectangular grid (1.000 m x 500 m). Taking into account different criteria such as precision, cost and ease of use, the fixed-area sampling unit looks like the most adequate among the four types investigated. [less ▲]

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See detailDie Devon/Karbongrenze bei Stockum (Rheinisches Schiefergebirge).
Clausen, C.D.; Korn, D; Feist, R et al

in Geologie und Paläontologie in Westfalen (1994), 29

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See detailPresence of gd TcR+ Tumor Infiltrating Lymphocytes (TILs) in One Case of Pediatric Osteogenic Sarcoma
Moutschen, Michel ULg; Jacobs, Nathalie ULg; Hoyoux, Claire ULg et al

in International Journal of Pediatric Hematology/Oncology (1994)

To characterize ab and gd TcR expression in cultured TILs from a metastatic osteogenic sar- coma.Patient and methods: TILs were isolated from a lung metastasis of an osteogenic sarcoma in a 16- year-old ... [more ▼]

To characterize ab and gd TcR expression in cultured TILs from a metastatic osteogenic sar- coma.Patient and methods: TILs were isolated from a lung metastasis of an osteogenic sarcoma in a 16- year-old female patient. Culture conditions were IL- 2 and anti-CD3 MoAb+IL-2. TcR expression and surface phenotype were studied by flow cytometry. 4h-chromium release assays were used to assess cytotoxicity. Results:IL-2-expanded tumor infiltrating T-lym- phocytes contained 50% gd TcR+ cells whereas gd TcR+ cells represented 20% of T-cells in cultures expanded with anti-CD3 MoAb+IL-2. The phenotype of gd TcR+ cells was CD3+, CD16-, CD4-, CD8-, and CD29+. AnH-CD3 MoAb + IL-2-expanded TILs mediated a significant lysis of thé autologous tumor and of an allogeneic lymphoma and displayed a higher activity against thé NK-sensitive target K562. In cold target experiments, unlabeled autol- ogous tumor cells blocked thé lysis of K562, indicating that MHC-unrestricted effectors were involved in thé lysis of both targets. Conclusions: This report demonstrates thé ex- pansion of large numbers of gd TcR+ T-cells after short-term culture of TILs from a metastatic os- teosarcoma. Further studies are needed to déter- mine thé role of gd TcR+ cells in host immune responses directed against this tumor. [less ▲]

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See detailFood Consumption in Belgium
Reginster-Haneuse, G.; Gosset, Christiane ULg

in Bibliotheca Nutritio et Dieta (1994), (51), 108-12

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See detailBGU III 897 et PSI XII 1228: συγγραφαί notariées publiques?
Straus, Jean ULg

in Zeitschrift für Papyrologie und Epigraphik (1994), 104

Le BGU III 987 = M. Chr. 269 est certainement une suggraphe notariée publique et le PSI XII 1228 en est vraisemblablement une, même s'il présente un élément constitutif importun.

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See detailEnergy Levels in Ar II
Quinet, Pascal ULg; Palmeri, P.; Biémont, Emile ULg et al

in Physical Review. A (1994), 49

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See detailL'analyse de la variance : une methode d'un autre âge ?
Claustriaux, Jean-Jacques ULg

in Biométrie-Praximétrie (1994), 34(2), 95-107

L'analyse de la variance est une ancienne méthode couramment utilisée pour le traitement statistique des données. Pour certains aspects, l'évolution de son utilisation pratique est présentée.

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See detailInsulin secretion, clearance and action before and after gastroplasty in severely obese subjects.
Letiexhe, Michel ULg; Scheen, André ULg; Gerard, Pascale ULg et al

in International Journal of Obesity & Related Metabolic Disorders (1994), 18(5), 295-300

This study investigated the effects of a drastic weight reduction on insulin secretion rate (ISR), insulin metabolic clearance rate (MCRI) and insulin sensitivity (SI) in severely obese subjects. A ... [more ▼]

This study investigated the effects of a drastic weight reduction on insulin secretion rate (ISR), insulin metabolic clearance rate (MCRI) and insulin sensitivity (SI) in severely obese subjects. A frequently sampled intravenous glucose tolerance test (FSIVGTT, 0.3 g/kg) was performed before and 8 +/- 1 months after a vertical ring gastroplasty in 12 overnight-fasted obese non-diabetic subjects; the results were compared to those obtained in 12 lean controls matched for age and sex. ISR was derived by deconvolution of plasma C-peptide levels; MCRI was obtained by dividing the area under the curve (AUC180 min) of ISR by the corresponding AUC of plasma insulin levels (IRI); the SI and the glucose effectiveness index (SG) were calculated by Bergman's minimal model. Before gastroplasty, obese subjects showed significantly higher ISR (P < 0.02), lower MCRI (P < 0.001), lower SI (P < 0.001) but similar SG when compared to lean controls. After gastroplasty (reduction of body weight from 104.8 +/- 3.8 to 73.4 +/- 3.6 kg and of BMI from 37.9 +/- 0.8 to 26.5 +/- 0.9 kg/m2; P < 0.001), ISR only decreased from 53,125 +/- 7968 to 42,302 +/- 3716 pmol/180 min (not significant) while AUC-IRI dramatically fell from 53,626 +/- 6378 to 21,111 +/- 2584 pmol.min/l; P < 0.001); consequently, MCRI markedly increased from 526 +/- 96 to 1257 +/- 150 ml/min/m2; P < 0.01). SI significantly rose from 3.12 +/- 0.45 to 7.10 +/- 1.20 x 10(-4) l/mU/min (P < 0.005) while SG remained unchanged.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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