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See detailOligodendrocytes: From Development to Demyelinated Lesion Repair
Rogister, Bernard ULg; Belachew, Shibeshih ULg; Moonen, Gustave ULg

in Acta Neurologica Belgica (1999), 99(1), 32-9

Spontaneous but incomplete remyelination is observed after a demyelinating lesion. We know since ten years now that oligodendrocyte progenitors, (OP) and totipotent neural stem cells remain present in the ... [more ▼]

Spontaneous but incomplete remyelination is observed after a demyelinating lesion. We know since ten years now that oligodendrocyte progenitors, (OP) and totipotent neural stem cells remain present in the central nervous system of adult mammals. [less ▲]

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See detailSt-Segment Elevation During Dobutamine Stress Testing Predicts Functional Recovery after Acute Myocardial Infarction
Pierard, Luc ULg; Lancellotti, Patrizio ULg; Kulbertus, Henri ULg

in American Heart Journal (1999), 137(3), 500-11

OBJECTIVES: The aims of this study were (1) to assess the relation between ST-segment elevation and wall motion response occurring during dobutamine testing and (2) to evaluate the usefulness of stress ... [more ▼]

OBJECTIVES: The aims of this study were (1) to assess the relation between ST-segment elevation and wall motion response occurring during dobutamine testing and (2) to evaluate the usefulness of stress-induced ST-segment elevation for predicting functional recovery after acute myocardial infarction. BACKGROUND: Clinical significance of stress-induced ST-segment elevation after acute myocardial infarction remains controversial. According to previous studies, it may reflect a larger infarcted area, depressed left ventricular function, left ventricular aneurysm, stress-induced dyskinesia, residual myocardial ischemia, or viability in the affected region. Whether transient ST-segment elevation occurring during dobutamine testing may predict functional recovery is unknown. METHODS AND RESULTS: We studied 38 patients who underwent dobutamine stress testing early (5 +/- 2 days) after a first acute myocardial infarction. Dobutamine was infused at increasing doses from 5 to a maximum of 40 microg/kg per minute, with the addition of up to 1 mg of atropine if the target rate could not be reached by dobutamine alone. Twelve-lead electrocardiography and cross-sectional echocardiography were continuously monitored throughout the test. Dobutamine-induced ST-segment elevation was defined as a new or worsening >/=1 mm elevation, 80 ms after J point, in >/=2 infarct-related leads. Quantitative angiography was available in all patients before hospital discharge. Follow-up resting echocardiography was recorded in all patients 12 to 18 months after the acute event. ST-segment elevation was observed in 20 of the 38 patients. There were no significant differences between patients with and those without dobutamine-induced ST-segment elevation in age, site of infarction, peak level of total creatine kinase enzyme, and use of thrombolytic therapy, angioplasty, or both. Persistent akinesis without change during dobutamine stress testing was more frequently observed in patients without ST elevation (P <. 05). A biphasic response during dobutamine testing was more frequently observed in patients with ST-segment elevation (P =.01). Multivariate analysis selected 2 independent variables associated with ST-segment elevation: a biphasic response during dobutamine stress (chi-square = 7.3; P =.007) and the minimal lumen diameter of the infarct-related vessel at quantitative angiography (chi-square = 5.5; P <.02). Functional recovery was demonstrated in 26 patients. Sensitivity of ST-segment elevation for the prediction of functional recovery was 69%, specificity 83%, positive predictive value 90%, and accuracy 74%. Two independent variables predicting functional recovery were selected: dobutamine-induced ST-segment elevation (chi-square = 9.1; P =.003) and a biphasic response during stress (chi-square = 6.15; P =.013). CONCLUSIONS: Dobutamine-induced ST-segment elevation in the infarct-related leads is an ancillary sign of viable myocardium in jeopardy. It has a high specificity and an acceptable sensitivity for the prediction of functional recovery after acute myocardial infarction. [less ▲]

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See detailReactive Oxygen Intermediate-Dependent Nf-Kappab Activation by Interleukin-1beta Requires 5-Lipoxygenase or Nadph Oxidase Activity
Bonizzi, Giuseppina; Piette, Jacques ULg; Haterte, Stéphanie ULg et al

in Molecular & Cellular Biology (1999), 19(3), 1950-60

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are ... [more ▼]

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are yet to be determined and might include 5-lipoxygenase (5-LOX) and NADPH oxidase. 5-LOX and 5-LOX activating protein (FLAP) are coexpressed in lymphoid cells but not in monocytic or epithelial cells. Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells. IL-1beta stimulation of epithelial cells did not generate any ROIs and NF-kappaB induction was not influenced by 5-LOX inhibitors. However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX-dependent NF-kappaB activation. In monocytic cells, IL-1beta treatment led to a production of ROIs which is independent of the 5-LOX enzyme but requires the NADPH oxidase activity. This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells. In conclusion, three different cell-specific pathways lead to NF-kappaB activation by IL-1beta: a pathway dependent on ROI production by 5-LOX in lymphoid cells, an ROI- and 5-LOX-independent pathway in epithelial cells, and a pathway requiring ROI production by NADPH oxidase in monocytic cells. [less ▲]

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See detailAdsorption of ampholytic diblock copolymers from dilute aqueous solution at the solid/liquid interface
Walter, H.; Harrats, C.; Müller-Buschbaum, P. et al

in Langmuir (1999), 15(4), 1260-1267

The adsorption of the ampholytic diblock copolymer poly(methacrylic acid)-block-poly((dimethylamino)ethyl methacrylate) (PMAA-b-PDMAEMA) and the corresponding homopolyelectrolytes of the two blocks, PMAA ... [more ▼]

The adsorption of the ampholytic diblock copolymer poly(methacrylic acid)-block-poly((dimethylamino)ethyl methacrylate) (PMAA-b-PDMAEMA) and the corresponding homopolyelectrolytes of the two blocks, PMAA and PDMAEMA, was investigated from dilute aqueous solution on silicon substrates. The adsorbed amount of polymer as a function of pH, polyampholyte concentration, and salt concentration in solution has been determined by ellipsometry at room temperature. As a function of pH the adsorbed amount reaches its maximum at the isoelectric point of the polyampholyte. Some adsorption takes place even in pH ranges where the surface charge has the same sign as the net charge of the polyampholyte. By variation of the polyampholyte concentration, typical adsorption isotherms were determined at several pH values. With increasing salt concentration the adsorbed amount increases, and above a critical concentration it diverges to very large values. The measured dependencies can be explained by the adsorption of one or the other of the two blocks depending on acidity and ionic strength and are in good agreement with theoretical predictions. Adsorption kinetics has also been studied in detail, and the diffusion coefficient of the polyampholyte toward the surface has been obtained in the early state of adsorption as a function of pH, polyampholyte concentration, and salt concentration. [less ▲]

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See detailMurine Matrix Metalloproteinase 9 Gene. 5'-Upstream Region Contains Cis-Acting Elements for Expression in Osteoclasts and Migrating Keratinocytes in Transgenic Mice
Munaut, Carine ULg; Salonurmi, T.; Kontusaari, S. et al

in Journal of Biological Chemistry (1999), 274(9), 5588-96

Knowledge about the regulation of cell lineage-specific expression of extracellular matrix metalloproteinases is limited. In the present work, the murine matrix metalloproteinase 9 (MMP-9) gene was shown ... [more ▼]

Knowledge about the regulation of cell lineage-specific expression of extracellular matrix metalloproteinases is limited. In the present work, the murine matrix metalloproteinase 9 (MMP-9) gene was shown to contain 13 exons, and the 2.8-kilobase pair upstream region was found to contain several common promoter elements including a TATA box-like motif, three GC boxes, four AP-1-like binding sites, an AP-2 site, and three PEA3 consensus sequences that may be important for basic activity of the gene. In order to identify cell-specific regulatory elements, constructs containing varying lengths of the upstream region in front of a LacZ reporter gene were made and studied for expression in transgenic mice generated by microinjection into fertilized oocytes. Analyses of the mice revealed that the presence of sequences between -2722 and -7745 allowed for expression in osteoclasts and migrating keratinocytes, i. e. cells that have been shown to normally express the enzyme in vivo. The results represent the first in vivo demonstration of the location of cell-specific control elements in a matrix metalloproteinase gene and show that element(s) regulating most cell-specific activities of 92-kDa type collagenase are located in the -2722 to -7745 base pair region. [less ▲]

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See detailStable Inhibition of Nuclear Factor Kappab in Cancer Cells Does Not Increase Sensitivity to Cytotoxic Drugs
Bentires-Alj, M.; Hellin, A. C.; Ameyar, M. et al

in Cancer Research (1999), 59(4), 811-5

Several reports indicated that nuclear factor kappaB (NF-kappaB) activation by cytokines, cytotoxic drugs, or ionizing radiation protects cells against apoptosis. Therefore, we investigated the ... [more ▼]

Several reports indicated that nuclear factor kappaB (NF-kappaB) activation by cytokines, cytotoxic drugs, or ionizing radiation protects cells against apoptosis. Therefore, we investigated the consequence of NF-kappaB inhibition on the efficiency of antineoplastic agents. HPB, HCT116, MCF7, and OVCAR-3 cells stably expressing a dominant negative IkappaBalpha inhibitor showed a decreased NF-kappaB activation following treatment with tumor necrosis factor a and various chemotherapeutic agents. However, there was no difference in survival between parental cells and cells expressing mutated IkappaBalpha. These studies suggest that, at least in these cell lines, stable NF-kappaB inhibition did not modify the response to cytotoxic drugs. [less ▲]

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See detailOpposing Actions of Intact and N-Terminal Fragments of the Human Prolactin/Growth Hormone Family Members on Angiogenesis: An Efficient Mechanism for the Regulation of Angiogenesis
Struman, Ingrid ULg; Bentzien, F.; Lee, H. et al

in Proceedings of the National Academy of Sciences of the United States of America (1999), 96(4), 1246-51

Angiogenesis, the process of development of a new microvasculature, is regulated by a balance of positive and negative factors. We show both in vivo and in vitro that the members of the human prolactin ... [more ▼]

Angiogenesis, the process of development of a new microvasculature, is regulated by a balance of positive and negative factors. We show both in vivo and in vitro that the members of the human prolactin/growth hormone family, i.e., human prolactin, human growth hormone, human placental lactogen, and human growth hormone variant are angiogenic whereas their respective 16-kDa N-terminal fragments are antiangiogenic. The opposite actions are regulated in part via activation or inhibition of mitogen-activated protein kinase signaling pathway. In addition, the N-terminal fragments stimulate expression of type 1 plasminogen activator inhibitor whereas the intact molecules have no effect, an observation consistent with the fragments acting via separate receptors. The concept that a single molecule encodes both angiogenic and antiangiogenic peptides represents an efficient model for regulating the balance of positive and negative factors controlling angiogenesis. This hypothesis has potential physiological importance for the control of the vascular connection between the fetal and maternal circulations in the placenta, where human prolactin, human placental lactogen, and human growth hormone variant are expressed. [less ▲]

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See detailAntibody Response to Glycoprotein E after Bovine Herpesvirus Type 1 Infection in Passively Immunised, Glycoprotein E-Negative Calves
Lemaire, Mylène; Schynts, Frédérick; Meyer, Gilles et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 144(7), 172-6

This study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (BHV-1) but without antibodies against glycoprotein E (gE) can produce an active ... [more ▼]

This study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (BHV-1) but without antibodies against glycoprotein E (gE) can produce an active antibody response to gE after a BHV-1 infection. Five calves received at birth colostrum from gE-seronegative cows which had been vaccinated two or three times with an inactivated BHV-1, gE-deleted marker vaccine. After inoculation with a wild-type virulent strain of BHV-1, all the passively immunised gE-negative calves shed virus in large amounts in their nasal secretions. All the calves seroconverted to gE within two to four weeks after inoculation and then had high levels of gE antibodies for at least four months. The development of an active cell-mediated immune response was also detected by in vitro BHV-1-specific interferon-gamma assays. All the calves were latently infected, because one of them re-excreted the virus spontaneously and the other four did so after being treated with dexamethasone. The results showed that under the conditions of this work the gE-negative marker could also distinguish between passively immunised and latently infected calves. [less ▲]

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See detailExpression of Mrna Encoding Alpha1e and Alpha1g Subunit in the Brain of a Rat Model of Absence Epilepsy
de Borman, B.; Lakaye, Bernard ULg; Minet, Arlette ULg et al

in Neuroreport (1999), 10(3), 569-74

Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding ... [more ▼]

Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding calcium channel alpha1E and alpha1G subunits was measured in the brain of genetic absence epilepsy rats from Strasbourg (GAERS). Using quantitative RT-PCR and in situ hybridization, no difference was found in alpha1G mRNA expression between GAERS and control animals, while a decreased expression of alpha1E was seen in the cerebellum and the brain stem of the GAERS. This phenomenon was not observed in young animals when the epileptic phenotype is not expressed. [less ▲]

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See detailIkappab-Alpha Enhances Transactivation by the Hoxb7 Homeodomain-Containing Protein
Chariot, Alain ULg; Princen, Frédéric; Gielen, Jacques et al

in Journal of Biological Chemistry (1999), 274(9), 5318-25

Combinatorial interactions between distinct transcription factors generate specificity in the controlled expression of target genes. In this report, we demonstrated that the HOXB7 homeodomain-containing ... [more ▼]

Combinatorial interactions between distinct transcription factors generate specificity in the controlled expression of target genes. In this report, we demonstrated that the HOXB7 homeodomain-containing protein, which plays a key role in development and differentiation, physically interacted in vitro with IkappaB-alpha, an inhibitor of NF-kappaB activity. This interaction was mediated by the IkappaB-alpha ankyrin repeats and C-terminal domain as well as by the HOXB7 N-terminal domain. In transient transfection experiments, IkappaB-alpha markedly increased HOXB7-dependent transcription from a reporter plasmid containing a homeodomain consensus-binding sequence. This report therefore showed a novel function for IkappaB-alpha, namely a positive regulation of transcriptional activation by homeodomain-containing proteins. [less ▲]

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See detailMechanical properties of weakly segregated block copolymers. 3. Influence of strain rate and temperature on tensile properties of poly(styrene-b-butyl methacrylate) diblock copolymers with different morphologies
Weidisch, Roland; Stamm, Manfred; Michler, G. H. et al

in Macromolecules (1999), 32(3), 742-750

Poly(styrene-b-butyl methacrylate) diblock copolymers, PS-b-PBMA, with different morphologies are investigated with respect to the influence of strain rate and temperature on tensile properties. In the ... [more ▼]

Poly(styrene-b-butyl methacrylate) diblock copolymers, PS-b-PBMA, with different morphologies are investigated with respect to the influence of strain rate and temperature on tensile properties. In the first part the mechanical properties of bicontinuous and perforated lamellar structure are compared with other morphologies. Diblock copolymers with bicontinuous structures (39% PS) show a much higher tensile strength as well as a higher strain at break than diblock copolymers with lamellar structures (50% PS). In the second part the dependence of tensile properties on strain rate and temperature are discussed for different morphologies. A diblock copolymer with a polystyrene content of 76% PS reveals hexagonally packed PBMA−cylinder, and the tensile strength, strain at break, and Young's modulus exceed the values of pure polystyrene at all measured strain rates. The interesting properties of PS-b-PBMA diblock copolymers are discussed with respect to the phase behavior, interface formation, and chain conformation. [less ▲]

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See detailFunctional neuroanatomy of hypnotic state
Maquet, Pierre ULg; Faymonville, Marie-Elisabeth ULg; Degueldre, Christian ULg et al

in Biological Psychiatry (1999), 45(3), 327-333

BACKGROUND: The aim of the present study was to describe the distribution of regional cerebral blood flow during the hypnotic state (HS) in humans, using positron-emission tomography (PET) and statistical ... [more ▼]

BACKGROUND: The aim of the present study was to describe the distribution of regional cerebral blood flow during the hypnotic state (HS) in humans, using positron-emission tomography (PET) and statistical parametric mapping. METHODS: The hypnotic state relied on revivification of pleasant autobiographical memories and was compared to imaging autobiographical material in "normal alertness." A group of 9 subjects under polygraphic monitoring received six H215O infusions and was scanned in the following order: alert-HS-HS-HS with color hallucination-HS with color hallucination-alert. PET data were analyzed using statistical parametric mapping (SPM95). RESULTS: The group analysis showed that hypnotic state is related to the activation of a widespread, mainly left-sided, set of cortical areas involving occipital, parietal, precentral, premotor, and ventrolateral prefrontal cortices and a few right-sided regions: occipital and anterior cingulate cortices. CONCLUSIONS: The pattern of activation during hypnotic state differs from those induced in normal subjects by the simple evocation of autobiographical memories. It shares many similarities with mental imagery, from which it differs by the relative deactivation of precuneus. [less ▲]

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See detailPharma-clinics. Comment je traite ... le zona
Nikkels, Arjen ULg; Pierard, Gérald ULg

in Revue Médicale de Liège (1999), 54(2), 71-75

The treatment of herpes zoster relies on the intravenous or oral administration of antiviral drugs. Oral aciclovir for shingles is hindered by its low bioavailability. New antiviral drugs with improved ... [more ▼]

The treatment of herpes zoster relies on the intravenous or oral administration of antiviral drugs. Oral aciclovir for shingles is hindered by its low bioavailability. New antiviral drugs with improved oral bioavailability (famciclovir and valaciclovir) allow a better efficacy. The opportuneness of treating herpes zoster is different in the immunocompetent and immunocompromised patients, and during childhood and pregnancy. [less ▲]

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See detailPrognostic factors in patients with Zollinger-Ellison syndrome and multiple endocrine neoplasia type 1. Groupe d'Etude des Neoplasies Endocriniennes Multiples (GENEM and groupe de Recherche et d'Etude du Syndrome de Zollinger-Ellison (GRESZE).
Cadiot, G.; Vuagnat, A.; Doukhan, I. et al

in Gastroenterology (1999), 116(2), 286-293

BACKGROUND & AIMS: Risk factors of metachronous liver metastases and death are not well known in patients with the Zollinger-Ellison syndrome and multiple endocrine neoplasia type 1. These factors were ... [more ▼]

BACKGROUND & AIMS: Risk factors of metachronous liver metastases and death are not well known in patients with the Zollinger-Ellison syndrome and multiple endocrine neoplasia type 1. These factors were retrospectively determined in 77 patients. METHODS: Data chart review was performed. RESULTS: Median follow-up was 102 months (range, 12-366). Surgery was performed on 48 patients, including 9 of the 10 patients with large pancreatic tumors (>/=3 cm). Liver metastases developed in 4 patients (40%) with large pancreatic tumors, in 3 (4.8%) without, and in 1 of the 4 patients with pancreatic tumors of unknown size; all had previously undergone surgery. The only independent factor associated with development of liver metastases identified by multivariate analysis was large pancreatic tumors (risk ratio, 29.0; 95% confidence interval [CI], 3. 2-260.7). Surgery was not selected. The probability of being free of liver metastases in the 63 patients without large pancreatic tumors was 96% (95% CI, 88-100) at 10 and 15 years. Thirteen (16.9%) patients died. The only independent factors of death selected by multivariate analysis were Zollinger-Ellison syndrome diagnosis before 1980 (risk ratio, 8.2; 95% CI, 1.7-40.6) and age at diagnosis (risk ratio/year, 1.08; 95% CI, 1.03-1.14). CONCLUSIONS: Large pancreatic tumors are predictive of the development of metachronous liver metastases, and surgery does not seem to prevent them. [less ▲]

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See detailThe Beta-Lactamase Cycle: A Tale of Selective Pressure and Bacterial Ingenuity
Matagne, André ULg; Dubus, Alain; Galleni, Moreno ULg et al

in Natural Product Reports (1999), 16(1), 1-19

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See detailLe cas clinique du mois. Prolapsus sur dégénérescence fibro-élastique. Les "cheveux blancs" de la valvule mitrale
Radermecker, M. A.; Hoffer, E.; Pierard, Luc ULg et al

in Revue Médicale de Liège (1999), 54(2), 76-8

We report the case of a 73-year-old woman who presents an acute mitral regurgitation secondary to fibro-elastic degenerescence. A mitral valvuloplasty is rapidly performed with complete disappearance of ... [more ▼]

We report the case of a 73-year-old woman who presents an acute mitral regurgitation secondary to fibro-elastic degenerescence. A mitral valvuloplasty is rapidly performed with complete disappearance of valvular insufficiency and the patient leaves the hospital ten days later. Degenerative mitral regurgitation is commonly seen in elderly patients. This constitutes an excellent indication for mitral valvuloplasty, rather than valve replacement. In our experience, mitral valvuloplasty leads to a complete correction of valvular regurgitation without neither stenosis, nor subvalvular obstruction. Left ventricular function is preserved, as subvalvular apparatus is conserved. This approach allows, in the absence of specific indication, anticoagulation withdrawn two to three months later. [less ▲]

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See detailCollective effects during crystal growth in the presence of mobile nonreactive impurities: experiments and simulations
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg; Cloots, Rudi ULg

in Journal of Crystal Growth (1999), 197(1-2), 317-324

The growth of a solid phase in a liquid medium containing randomly dispersed mesoscopic impurities is discussed. Particles represent hindrances for the growth front. They can remain static or can be ... [more ▼]

The growth of a solid phase in a liquid medium containing randomly dispersed mesoscopic impurities is discussed. Particles represent hindrances for the growth front. They can remain static or can be pushed by the advancing interface. The physics of the collective particle motion is outlined. Experiments with A12O3-SiO2 impurities in KC1 crystal serve as illustrative examples. The collective effects due to the particle concentration are investigated through a kinetic growth model. [less ▲]

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See detailLoss of the Y Chromosome in Bone Marrow Cells: Results on 1907 Consecutive Cases of Leukaemia and Preleukaemia
Herens, Christian ULg; Brasseur, Edmond ULg; Jamar, Michelle ULg et al

in Clinical & Laboratory Haematology (1999), 21(1), 17-20

Loss of the Y chromosome with a resulting 45, X0 karyotype is observed in normal bone marrow cells of elderly males but also in haematological malignancies. Whether Y loss in neoplastic cells is related ... [more ▼]

Loss of the Y chromosome with a resulting 45, X0 karyotype is observed in normal bone marrow cells of elderly males but also in haematological malignancies. Whether Y loss in neoplastic cells is related to the process seen in normal ageing or is part of the carcinogenic process is unknown. The present study concerns the cytogenetic data from 1907 consecutive leukaemic or preleukaemic male patients with special regard to the presence or absence of the Y chromosome. Sixty-five patients (3.4%) had a 45, X-Y clone in their bone marrow (BM) cells. Loss of Y was rare below the age of 50 but increased in older patients, reaching 25% of the men over 80. Sixteen patients (0.08%) had more than 90% X0 cells in their BM. A correlation between Y loss and leukaemia could be established in seven cases, three of which were acute myeloid leukaemia M2 subtype where -Y is known to be a secondary event. In three other cases, -Y was part of a complex karyotype. Only one patient exhibited a 45, X0 karyotype, with no other rearrangement, that could be positively correlated with the neoplastic process. [less ▲]

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See detailEffects of Training on Exercise-Induced Muscle Damage and Interleukin 6 Production
Croisier, Jean-Louis ULg; Camus, G.; Venneman, Ingrid ULg et al

in Muscle & Nerve (1999), 22(2), 208-12

To address the question of whether the increased plasma concentration of interleukin 6 (IL-6) following strenuous muscular work could be related to exercise-induced muscle damage, 5 moderately active male ... [more ▼]

To address the question of whether the increased plasma concentration of interleukin 6 (IL-6) following strenuous muscular work could be related to exercise-induced muscle damage, 5 moderately active male volunteers underwent two isokinetic exercise sessions in the eccentric mode, separated by a period of 3 weeks during which the subjects underwent five training sessions. Before training, exercise was followed by severe muscle pain (delayed-onset muscle soreness; DOMS), and by significant increases in plasma IL-6 level and serum myoglobin concentration (SMb) (P < 0.001). After training, postexercise DOMS and SMb values were significantly lower than those measured before training. There was no significant difference between plasma IL-6 levels measured at the same time points before and after training. We conclude that the hypothetical relationship between exercise-induced muscle damage and increased postexercise levels of circulating IL-6 is not substantiated by the present results. [less ▲]

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See detailChronic Herpes Simplex Virus Type I Glossitis in an Immunocompromised Man
Nikkels, Arjen ULg; Pierard, Gérald ULg

in British Journal of Dermatology (1999), 140(2), 343-346

Herpes simplex virus (HSV) type 1 (HSV-1) infection of the tongue commonly accompanies acute primary herpetic gingivostomatitis. However, recurrent infection of the tongue is exceptional and is restricted ... [more ▼]

Herpes simplex virus (HSV) type 1 (HSV-1) infection of the tongue commonly accompanies acute primary herpetic gingivostomatitis. However, recurrent infection of the tongue is exceptional and is restricted to immunocompromised individuals. A 57-year-old man with corticosteroid-dependent chronic obstructive pulmonary disease and sciatica presented with a chronic median glossitis due to HSV-1. The main clinical and histological feature was massive necrosis of the entire mucosa. Immunohistochemistry demonstrated a considerable amount of HSV gB, gC and gD envelope glycoproteins dispersed in the chorion. In contrast, HSV-1 DNA was detected only in a limited number of epithelial cells using in situ hybridization. The extent of necrosis and the pattern of viral DNA and envelope protein distribution represent unique features of median herpetic glossitis, which are not found in more common types of HSV infection. [less ▲]

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See detailMechanistic Diversity of Beta-Lactamases
Frère, Jean-Marie ULg; Dubus, Alain; Galleni, Moreno ULg et al

in Biochemical Society Transactions (1999), 27(2), 58-63

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See detailControl of Dioscorea alata microtubers dormancy and germination by jasmonic acid
Bazabakana, R.; Fauconnier, Marie-Laure ULg; Jaziri, M. et al

in Plant Growth Regulation (1999), 27(2), 113-117

Effects of appling exogenous jasmonic acid (JA) on the germination of Dioscorea alata L. microtubers were examined on Murashige and Skoog (MS) medium. Microtuber germination was promoted by JA (0.1 and 1 ... [more ▼]

Effects of appling exogenous jasmonic acid (JA) on the germination of Dioscorea alata L. microtubers were examined on Murashige and Skoog (MS) medium. Microtuber germination was promoted by JA (0.1 and 1 µM) supplemented to the culture medium but higher concentrations (30 and 100 µM) completely inhibited germination. When these inhibited microtubers were transferred to hormone-free medium, germination resumed. After transfer to greenhouse conditions, almost all plants (95%) from tubers previously cultivated on MS medium with 100 µM JA survived and all acclimatized plants had produced tubers after 8 months. It is concluded that depending on JA concentration, both the germination and dormancy processes in D. alata microtubers were affected. The release from dormancy is easily obtained by transferring dormant microtubers to hormone-free medium. [less ▲]

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See detailTypical Presentation of Intramural Aortic Haemorrhage (Iah) with Evidence of Intimal Tear at Mri and Angiography
RADERMECKER, Marc ULg; LAVIGNE, Jean-Paul ULg; Limet, Raymond ULg

in Acta Chirurgica Belgica (1999), 99(1), 44-6

A typical appearance of IAH was evidenced by CT and TEE in a 56-year-old hypertensive female suspected of developping classical acute aortic dissection (AAD). Further examination with MRI and aortography ... [more ▼]

A typical appearance of IAH was evidenced by CT and TEE in a 56-year-old hypertensive female suspected of developping classical acute aortic dissection (AAD). Further examination with MRI and aortography showed unequivocally the presence of an intimal tear in the aortic arch. This coexistence of intimal tear has never been evidenced preoperatively in patients with IAH. This observation demonstrates at the outset that IAH is part of the spectrum of AAD. [less ▲]

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See detailEeuwige vragen? Over Mooi, maar dat is het woord niet van Rutger Kopland
Spinoy, Erik ULg

in Neerlandica Extra Muros (1999), 37(1), 53-58

This article is a critical survey of the literary views held by the Dutch poet Rutger Kopland.

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See detailSubstituts des globules rouges: émulsions de fluorocarbures et solutions d'hémoglobine
Remy, Bernadette ULg; Deby, Ginette ULg; D'Ans, V. et al

in Annales Françaises d'Anesthésie et de Réanimation (1999), 18(2), 211-24

OBJECTIVE: To review the current data on perfluorocarbon (PFC) emulsions and haemoglobin (Hb) solutions. DATA SOURCES: For this paper we analysed the literature using Medline search along with major ... [more ▼]

OBJECTIVE: To review the current data on perfluorocarbon (PFC) emulsions and haemoglobin (Hb) solutions. DATA SOURCES: For this paper we analysed the literature using Medline search along with major review articles. DATA SELECTION AND EXTRACTION: The collected articles were reviewed and selected according to their quality and originality. DATA SYNTHESIS: PFCs are synthetic fluorinated hydrocarbons capable of dissolving, at increased FIO2, large amounts of oxygen. They deliver oxygen at tissular level, and are administered as emulsions containing particles of around 0.1 micron, reaching the smallest vessels. They are exhaled unchanged by the lungs within 7 days. The first clinically used PFC was Fluosol-DA 20%. Currently, Oxyfluor 40% and Oxygent 60% are under evaluation. PFCs are not true blood substitutes, but rather a means to support tissue oxygenation during extreme haemodilution. Solutions of free Hb do not require compatibility testing and are fully saturated with oxygen at ambient FIO2. Hb is either human, bovine or recombinant Hb. In order to maintain adequate intravascular half-life and affinity for oxygen, the Hb molecules are modified by internal cross-linking, polymerisation and encapsulation. After promising results using animal models, some of these modified Hb solutions are now undergoing phase III clinical trials. Among these, diaspirin cross-linked haemoglobin (DCLHb) has been tested in cardiac and orthopaedic surgery, as well as in trauma patients. The initial results of these multicentre trials are currently being analysed. [less ▲]

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See detailBiodegradable and biocompatible inorganic-organic hybrid materials: 4. Effect of acid content and water content on the incorporation of aliphatic polyesters into silica by the sol-gel process
Tian, Dong; Blacher, Silvia ULg; Jérôme, Robert ULg

in Polymer (1999), 40(4), 951-957

The extent of poly -caprolactone (PCL) incorporation into silica networks prepared by the sol-gel process depends on the HCl:tetraethoxysilane (TEOS) molar ratio and the H2O:TEOS molar ratio, as well. The ... [more ▼]

The extent of poly -caprolactone (PCL) incorporation into silica networks prepared by the sol-gel process depends on the HCl:tetraethoxysilane (TEOS) molar ratio and the H2O:TEOS molar ratio, as well. The PCL incorporation increases with the concentration of the acid used as the catalyst. Dynamic mechanical analysis (DMA) indicates that increasing the acid concentration or decreasing the water content results in a higher glass transition temperature for the organic component in the hybrid materials, whereas the modulus does not change significantly. Small-angle X-ray scattering (SAXS) data agree with a nanoscopic phase separation of the two constitutive components: the organic polymer and the silica network. The characteristic length of this two-phase structure increases with acid content (except for the lower HCl:TEOS molar ratio of 0.05) and with water content (except for the higher H2O:TEOS molar ratio of 2.0). The structure of the PCL/SiO2 interface is mass fractal and the structure is more open when the acid content is lower or the water content higher. [less ▲]

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See detailWater invasion, freezing and thawing in cementitious materials
Ausloos, Marcel ULg; Salmon, E.; Vandewalle, Nicolas ULg

in Cement & Concrete Research (1999), 29(2), 209-213

A simple model of fluid invasion, freezing, and thawing in a porous medium was developed using an "invasion percolation-like" approach. The fluid freezing process is considered as destroying the internal ... [more ▼]

A simple model of fluid invasion, freezing, and thawing in a porous medium was developed using an "invasion percolation-like" approach. The fluid freezing process is considered as destroying the internal structure of the porous medium. The evolution of the pore size distribution after several invasionfrostthaw events is investigated numerically. Those results and qualitatively consistent with experimental measurements on fiberreinforced cements. [less ▲]

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See detailoncologie gastro-intestinale
Frei, A.; Coucke, Philippe ULg; Felley, C. et al

in Revue Médicale Suisse (1999), 760

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See detailInteraction between Class B Beta-Lactamases and Suicide Substrates of Active-Site Serine Beta-Lactamases
Prosperi-Meys, C.; Llabres, Gabriel ULg; De Seny, Dominique ULg et al

in FEBS Letters (1999), 443(2), 109-11

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders ... [more ▼]

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders of magnitude. A comparison of the kinetic parameters for the alpha and beta isomers of 6-iodopenicillanic acid shows that there is no general preference for the alpha isomer and that the efficient hydrolysis of imipenem by these enzymes must rest on other factors. [less ▲]

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See detailPolar stratospheric descent of NOy and CO and Arctic denitrification during winter 1992-1993
Rinsland, C. P.; Salawitch, R. J.; Gunson, M. R. et al

in Journal Of Geophysical Research. Atmospheres (1999), 104(D1), 1847-1861

Observations inside the November 1994 Antarctic stratospheric vortex and inside the April 1993 remnant Arctic stratospheric vortex by the Atmospheric Trace Molecule Spectroscopy (ATMOS) Fourier transform ... [more ▼]

Observations inside the November 1994 Antarctic stratospheric vortex and inside the April 1993 remnant Arctic stratospheric vortex by the Atmospheric Trace Molecule Spectroscopy (ATMOS) Fourier transform spectrometer are reported. In both instances, elevated volume mixing ratios (VMRs) of carbon monoxide (CO) were measured. A peak Antarctic CO VMR of 60 ppbv (where 1 ppbv = 10(-9) per unit volume) was measured at a potential temperature (Theta) of 710 K (similar to 27 km), about 1 km below the altitude of a pocket of elevated NOy (total reactive nitrogen) at a deep minimum in N2O (<5 ppbv). The Arctic observations also show a region of elevated vortex CO with a peak VMR of 90 ppbv at 630-670 K (similar to 25 km) but no corresponding enhancement in NOy, perhaps because of stronger dynamical activity in the northern hemisphere polar winter and/or interannual variability in the production of mesospheric NO. By comparing vortex and extravortex observations of NOy obtained at the same N2O VMR, Arctic vortex denitrification of 5 +/- 2 ppbv at 470 K (similar to 18 km) is inferred. We show that our conclusion of substantial Arctic winter 1992-1993 denitrification is robust by comparing our extravortex observations with previous polar measurements obtained over a wide range of winter conditions. Correlations of NOy with N2O measured at the same Theta by ATMOS in the Arctic vortex and at midlatitudes on board the ER-2 aircraft several weeks later lie along the same mixing line. The result demonstrates the consistency of the two data sets and confirms that the ER-2 sampled fragments of the denitrified Arctic vortex following its breakup, An analysis of the ATMOS Arctic measurements of total hydrogen shows no evidence for significant dehydration inside the vortex. [less ▲]

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See detailModifier l'utilisation/la destination d'un bâtiment, en Région wallonne
Delnoy, Michel ULg

in Immobilier (1999), 2

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See detailSynthesis and bulk characterization of new P(CB-b-S) diblock copolymers
Stone, V. W.; Jonas, Alain M; Legras, Roger et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1999), 37(2), 233-244

Strongly asymmetric chlorinated polybutadiene-b-polystyrene, [P((CB)x-b-(PS)y)] diblock copolymers with increasing x/(x + y) ratios (up to 5.2 mol %) have been synthesized by the selective chlorination of ... [more ▼]

Strongly asymmetric chlorinated polybutadiene-b-polystyrene, [P((CB)x-b-(PS)y)] diblock copolymers with increasing x/(x + y) ratios (up to 5.2 mol %) have been synthesized by the selective chlorination of the polybutadiene (PB) block in solution. Chlorination has been performed in anhydrous dichloromethane added with an antioxidant [2,2-methylenebis-(6-tert-butyl-4-methyl-phenol)], at -50°C, under a continuous Ar flow and in the dark. Under the optimized experimental conditions, the PB chlorination is not complete, but the PS block is left unmodified. Even in the presence of a large chlorine excess (Cl2/butene unit molar ratio of 2.5), the experimental degree of chlorination of homo PB does not exceed 85%. The chlorinated copolymers have been characterized by 1H-NMR, IR spectroscopy, size-exclusion chromatography, and elemental analysis. The chlorinated copolymers have also been studied by DSC and SAXS after annealing at 150°C. Although at this temperature the parent homopolymers are immiscible, no microphase separation has been observed for the block copolymers. [less ▲]

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See detailControlling the electrografting of polymers onto transition metal surfaces through solvent vs monomer adsorption
Crispin, Xavier; Lazzaroni, Roberto; Geskin, Victor et al

in Journal of the American Chemical Society (1999), 121(1), 167-187

Electropolymerization of methacrylic monomers opens the possibility of chemically grafting a wide range of polymers onto transition metal surfaces. In this work, the electropolymerization of ... [more ▼]

Electropolymerization of methacrylic monomers opens the possibility of chemically grafting a wide range of polymers onto transition metal surfaces. In this work, the electropolymerization of polyacrylonitrile and polyethyl acrylate is studied in different solvents; we experimentally confirm that the choice of solvent is a critical parameter for obtaining electrografted polymers. A density-functional theory−based study modeling the interaction of solvent (acetonitrile, dimethylformamide, and pyridine) or monomer (acrylonitrile and ethyl acrylate) molecules with the Ni(100) metal surface provides the means to classify the organic molecules with respect to their ability to interact with the surface. The surface binding-energy difference between monomer and solvent is introduced in a Frumkin-type isotherm. This allows us to rationalize the experimental observations in terms of a competitive adsorption at the metal surface between the monomer and the solvent. The first step in the electrografting mechanism thus appears to be the chemisorption of the monomer at the electrode surface before cathodic polarization is applied; the chemisorbed monomer is therefore the first species reduced, giving rise to an adsorbed reactive intermediate, which is thus able to start the polymerization of a grafted chain. [less ▲]

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See detailControlled radical polymerization of (meth)acrylates by ATRP with NiBr2(PPh3)2 as catalyst
Moineau, Georges; Minet, Michaël; Dubois, Philippe ULg et al

in Macromolecules (1999), 32(1), 27-35

NiBr2(PPh3)2 has been found to be an efficient catalyst for the ATRP of methyl methacrylate (MMA) and n-butyl acrylate (n-BuA) initiated by an alkyl halide in the absence of any activator (e.g., Lewis ... [more ▼]

NiBr2(PPh3)2 has been found to be an efficient catalyst for the ATRP of methyl methacrylate (MMA) and n-butyl acrylate (n-BuA) initiated by an alkyl halide in the absence of any activator (e.g., Lewis acid) at 85 °C. The molecular weight distribution of the poly(meth)acrylates is narrow (Mw/Mn = 1.1−1.4). However, some side reactions (more likely coupling reactions) are observed at high monomer conversions in the case of n-BuA. An excess of PPh3 has proved to increase the polymerization rate of MMA while preserving the control of the molecular parameters. When the catalyst/initiator molar ratio is too small (e.g., 0.05), the polymerization rate decreases, the polydispersity increases, and the initiation is less efficient. α-Acid and α-hydroxyl end groups have been successfully attached to the chains by using functional initiators, such as 2-bromo-2-methylpropionic acid and 2,2‘,2‘ ‘-tribromoethanol. Reactivity ratios for the MMA/n-BuA comonomer pair have been measured and found to be close to the values observed for a conventional free-radical polymerization. Diethyl meso-2,5-dibromoadipate has been used as a difunctional initiator for the n-BuA polymerization, leading to α,ω-bromo-poly(n-BuA) of narrow molecular weight distribution. Finally, the thermal stability of PMMA is consistent with the lack of termination reactions, while Tg is as high as 125 °C. [less ▲]

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See detailStructure-second-order polarizability relationship in chromophores incorporating a spacer: a joint experimental and theoretical study
Maertens, Christophe; Detrembleur, Christophe ULg; Dubois, Philippe ULg et al

in Chemistry : A European Journal (1999), 5(1), 369-380

A joint theoretical and experimental study is reported on the structure-polarizability relationship in a novel type of push-pull conjugated system which contains a spacer within the conjugated backbone ... [more ▼]

A joint theoretical and experimental study is reported on the structure-polarizability relationship in a novel type of push-pull conjugated system which contains a spacer within the conjugated backbone. The chromo-phores are based on a dithienyl conjugation pathway, spaced by a keto group, and selectively end-capped by a donor and an acceptor. Four chromophores were synthesized, in which the strength of the acceptor group and the length of the conjugated path were varied. The electronic properties of two model compounds were studied theoretically by means of correlated quantum-chemical calculations. The degree of ground-state polarization was varied in the calculations by the application of an external electric field and varied experimentally by the modification of the dielectric constant of a binary mixture of solvents. The linear and nonlinear optical properties of the compounds (measured in solution by hyper-Rayleigh scattering) are discussed in detail. [less ▲]

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See detailAbundance correlations in mildly metal-poor stars
Jehin, Emmanuel ULg; Magain, Pierre ULg; Neuforge, Corinne et al

in Astronomy and Astrophysics (1999), 341

Accurate relative abundances have been obtained for a sample of 21 mildly metal-poor stars from the analysis of high resolution and high signal-to-noise spectra. In order to reach the highest coherence ... [more ▼]

Accurate relative abundances have been obtained for a sample of 21 mildly metal-poor stars from the analysis of high resolution and high signal-to-noise spectra. In order to reach the highest coherence and internal precision, lines with similar dependency on the stellar atmospheric parameters were selected, and the analysis was carried out in a strictly differential way within the sample. With these accurate results, correlations between relative abundances have been searched for, with a special emphasis on the neutron capture elements. This analysis shows that the r elements are closely correlated to the alpha elements, which is in agreement with the generally accepted idea that the r-process takes place during the explosion of massive stars. The situation is more complex as far as the s elements are concerned. Their relation with the alpha elements is not linear. In a first group of stars, the relative abundance of the s elements increases only slightly with the alpha elements overabundance until the latter reaches a maximum value. For the second group, the s elements show a rather large range of enhancement and a constant (and maximum) value of the alpha elements overabundance. This peculiar behaviour leads us to distinguish between two sub-populations of metal-poor stars, namely Pop IIa (first group) and Pop IIb (second group). We suggest a scenario of formation of metal-poor stars based on two distinct phases of chemical enrichment, a first phase essentially consisting in supernova explosions of massive stars, and a second phase where the enrichment is provided by stellar winds from intermediate mass stars. More specifically, we assume that all thick disk and field halo stars were born in globular clusters, from which they escaped, either during an early disruption of the cluster (Pop IIa) or, later, through an evaporation process (Pop IIb). Based on observations obtained at the European Southern Observatory, La Silla, Chile. [less ▲]

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See detailRecent achievements in anionic polymerization of (meth)acrylates
Jérôme, Robert ULg; Teyssié, Philippe; Vuillemin, Bruno et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1999), 37(1), 1-10

The constant progress of the anionic polymerization of (meth)acrylates is discussed from both the fundamental and practical points of view. A special attention is paid to the improved macromolecular ... [more ▼]

The constant progress of the anionic polymerization of (meth)acrylates is discussed from both the fundamental and practical points of view. A special attention is paid to the improved macromolecular engineering of (meth)acrylate-based (co)polymers. The resulting most important materials and the scaling-up process needed for their production are also emphasized. The recent developments witness for the healthy state of the anionic polymerization of these polar monomers. [less ▲]

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See detailRegional Distribution and Control of Tyrosine Hydroxylase Activity in the Quail Brain
Baillien, M.; Foidart, Agnès ULg; Balthazart, Jacques ULg

in Brain Research Bulletin (1999), 48(1), 3-17

Tyrosine hydroxylase (TH) activity, the rate-limiting step in the synthesis of catecholamines, was quantified in the preoptic area-hypothalamus of adult male Japanese quail by a new assay measuring the ... [more ▼]

Tyrosine hydroxylase (TH) activity, the rate-limiting step in the synthesis of catecholamines, was quantified in the preoptic area-hypothalamus of adult male Japanese quail by a new assay measuring the tritiated water production from 3,5-[3H]-L-tyrosine. Maximal levels of activity were observed at a 20-25 microM concentration of substrate, with more than 50% inhibition of the activity being recorded at a 100 microM concentration. TH activity was linear as a function of the incubation time during the first 20 min and maximal at a pH of 6.0. TH was heterogeneously distributed in the quail brain with highest levels of activity being found (in decreasing order) in the mesencephalon, diencephalon, and telencephalon. Given the large size of the telencephalon, this is the brain area that contains, as a whole, the highest level of enzyme activity. TH inhibitors that have been well-characterized in mammals, such as 3-iodo-L-tyrosine and L-alpha-methyl-p-tyrosine (AMPT) completely inhibited the enzyme activity at a 100 microM concentration. In mammals, the accumulation of catecholamines exerts a negative feedback control on TH activity. Similar controls were observed in the quail brain. Two inhibitors of the DOPA decarboxylase that should lead to accumulation of DOPA depressed TH activity by 60% or more, and the inhibitor of the dopamine beta-hydroxylase, fusaric acid that should cause an accumulation of dopamine, suppressed 90% of the TH activity. The addition of exogenous DOPA, dopamine, or norepinephrine to the brain homogenates also strongly inhibited TH activity, independently confirming the feedback effects of the enzyme products on the enzyme activity. These data demonstrate that TH activity in the quail brain is heterogeneously distributed and acutely regulated, as it is in mammals, by the accumulation of its products and of the derived catecholamines. [less ▲]

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See detailOn the Motion of Trapped Particles in the Vicinity of Corotation Centers
Beaugé, C.; Lemaitre, Anne; Jancart, Sylvie ULg

in Celestial Mechanics and Dynamical Astronomy (1999), 73

In the present paper we analyse the motion of a massless particle during the capture process in an exterior mean-motion resonance under the effects of an external dissipative force. In particular, we ... [more ▼]

In the present paper we analyse the motion of a massless particle during the capture process in an exterior mean-motion resonance under the effects of an external dissipative force. In particular, we study the orbital evolution from its initial approach to the commensurability up to the final nesting place in the periodic orbit around the equilibrium solution. [less ▲]

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See detailSynthesis of well-defined poly(alkyl methacrylate)-graft-polylactone by sequential living polymerization
Mecerreyes, David; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecular Chemistry and Physics (1999), 200(1), 156-165

A novel combination of living polymerization reactions has been proposed for the controlled synthesis of poly(alkyl methacrylate)-graft-polylactones. This strategy relies upon the sequential living ... [more ▼]

A novel combination of living polymerization reactions has been proposed for the controlled synthesis of poly(alkyl methacrylate)-graft-polylactones. This strategy relies upon the sequential living polymerization of alkyl methacrylates and aliphatic lactones, with an intermediate chemical transformation for shifting from the first mechanism to the second one. In the first step, an alkyl methacrylate (methyl and butyl) is copolymerized with 2-trimethylsiloxyethyl methacrylate (TMSEMA). This living anionic polymerization is initiated with diphenylhexyllithium( DPHLi) in the presence of a μ-ligand, lithium chloride, in THF at - 78 °C. The trimethylsiloxy groups are then hydrolyzed with release of hydroxyl groups which are reacted with triethylaluminum in order to form a multifunctional macroinitiator of the Al alkoxide type. The second step consists of the ring opening polymerization (ROP) of aliphatic lactones (ε-caprolactone, J-valerolactone and 1,4,8-trioxaspiro[4.6]-9-undecanone (TOSUO)) with the formation of novel graft copolymers. As a result of the livingness of both the anionic and the ROP polymerization steps, the molecular weight of both the main backbone and the grafts is predictable, the apparent polydispersity is narrow ( from 1.05 to 1.30) and the grafting density can be controlled being dependent on the distribution of the hydroxyl groups within the precursor backbone. [less ▲]

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See detailStructural and functional organization of the fengycin synthetase multienzyme system from Bacillus subtilis b213 and A1/3
Steller, S.; Vollenbroich, D.; Leenders, F. et al

in Chemistry & Biology (1999), 6(31-41),

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See detailPregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-partum dairy cows
Dosogne, H.; Burvenich, Christian; Freeman, A. E. et al

in Veterinary Immunology and Immunopathology (1999), 67(1), 47-54

Phagocytosis and oxidative burst activity of polymorphonuclear neutrophil leukocytes (PMN) isolated from blood and pregnancy-associated glycoprotein (bPAG) concentrations in plasma were evaluated in two ... [more ▼]

Phagocytosis and oxidative burst activity of polymorphonuclear neutrophil leukocytes (PMN) isolated from blood and pregnancy-associated glycoprotein (bPAG) concentrations in plasma were evaluated in two longitudinal studies in dairy cows from 3 weeks before until 5 weeks after calving, carried out in the United States and in Europe. Ingestion of Staphylococcus aureus by blood PMN increased during the first week after calving and normalised 3 weeks post-partum. Phagocytosis of Escherichia coli did not change in the early post-partum period. In both studies, a significant decrease in oxidative burst activity of PMN was observed between 1 and 3 weeks after calving. In all cows, a very significant increase in plasma bPAG concentration was found between 1 week before and 2 weeks after calving. The peak of bPAG concentration in plasma immediately preceded the alterations of blood PMN functions. These results suggest that bPAG may be associated with inhibition of PMN function of dairy cows during the early post-partum period. [less ▲]

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See detailAtheroemboles et chirurgie cardiaque: importance de la mise au point préopératoire
Kolh, Philippe ULg; Torchiana, D.; Buckley, M.

in Revue Médicale de Liège (1999), 54(1), 43-8

OBJECTIVE: Atheroembolization is a recognized complication of cardiac surgical procedures, and has been implicated in postoperative stroke, renal failure, multiorgan failure, and death. Preoperative ... [more ▼]

OBJECTIVE: Atheroembolization is a recognized complication of cardiac surgical procedures, and has been implicated in postoperative stroke, renal failure, multiorgan failure, and death. Preoperative identification of patients at risk for developing atheroemboli is essential. The aim of this study was to determine preoperative risk factors for atheroemboli and to assess the postoperative course of the patients who developed atheroembolic syndrome. METHODS: A retrospective record review was conducted. From 1/1990 to 12/1994, 5.486 patients underwent coronary artery bypass grafting (CABG), valve operations, or other cardiac surgical procedures at the Massachusetts General Hospital. Among this population, 107 patients (1.9%) developed atheroembolic syndrome. There were compared to a concurrent population of 925 patients operated during the same period. RESULTS: Patients who developed atheroemboli were older, with an increased incidence (p < 0.01) of hypertension, cerebrovascular disease, and aortoiliac disease. Many had a complicated course after catheterization, with renal insufficiency (35%) and evidence of peripheral emboli (12%). Average Intensive Care Unit stay, hospital stay, and hospital cost of these patients were 16.8 days, 48.4 days and 88,000 respectively, compared to 1.5 days, 9.6 days and 23,000 for the concurrent population. Of these 107 patients only two were discharged home, the others either died (48 patients, or 25% of all cardiac surgical deaths during this period), or rehabilitation or chronic hospital facilities. Twenty-seven autopsies were performed and invariably showed a diffusely diseased aorta, with calcification, mural thrombus, and ulceration. CONCLUSIONS: Atheroembolization during cardiac surgical procedures has profound medical and economic consequences. Because of the diffuse nature of aortic disease, measures approaching the disease as a local process are likely to be unsuccessful. Appropriate evaluation would ideally identify patients with extensive aortic atheromatous disease, prior to rather than during surgery. [less ▲]

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See detailRegional brain activity during tasks devoted to the central executive of working memory
Collette, Fabienne ULg; Salmon, Eric ULg; Van der Linden, Martial ULg et al

in Cognitive Brain Research (1999), 7(3), 411-417

Most previous PET studies investigating the central executive (CE) component of working memory found activation in the prefrontal cortex. However, the tasks used did not always permit to distinguish ... [more ▼]

Most previous PET studies investigating the central executive (CE) component of working memory found activation in the prefrontal cortex. However, the tasks used did not always permit to distinguish precisely the functions of the CE from the storage function of the slave systems. The aim of the present study was to isolate brain areas that subserve manipulation of information by the CE when the influence of storage function was removed. A PET activation study was performed with four cognitive tasks, crossing conditions of temporary storage and manipulation of information. The manipulation of information induced an activation in the right (BA 10/46) and left (BA 9/6) middle frontal gyrus and in the left parietal area (BA7). The interaction between the storage and manipulation conditions did not reveal any significant changes in activation. These results are in agreement with the hypothesis that CE functions are distributed between anterior and posterior brain areas, but could also reflect a simultaneous involvement of controlled (frontal) and automatic (parietal) attentional systems. In the other hand, the absence of interaction between the storage and manipulation conditions demonstrates that the CE is not necessarily related to the presence of a memory load. [less ▲]

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See detailPhase segregation in binary sandpiles on fractal bases
Vandewalle, Nicolas ULg; D'hulst, R.; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1999), 59(1), 631-635

We have built experimentally and have numerically studied sandpiles on a base having a prefractal perimeter. This type of perimeter induces the formation of quite complex pile shapes characterized by both ... [more ▼]

We have built experimentally and have numerically studied sandpiles on a base having a prefractal perimeter. This type of perimeter induces the formation of quite complex pile shapes characterized by both ridges and valleys. The effects of a fractal base on the phase segregation of a binary granular system have been investigated. Both demixing and self-stratification phenomena have been investigated. It is found that the demixing of binary granular piles is enhanced by the prefractal perimeter character. The concentration profiles are given. This is briefly discussed in terms of length scale selection. [less ▲]

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See detailMorphometrical study of the equine navicular bone: age-related changes and influence of exercise
Gabriel, Annick ULg; Detilleux, Johann ULg; Jolly, Sandra ULg et al

in Veterinary Research Communications (1999), 23(1), 15-40

Navicular bones from the four limbs of 95 horses, classified in 9 categories, were studied. The effects of age on navicular bone morphometry and histomorphometry were estimated, after adjustment of the ... [more ▼]

Navicular bones from the four limbs of 95 horses, classified in 9 categories, were studied. The effects of age on navicular bone morphometry and histomorphometry were estimated, after adjustment of the data to even out the effects of front and rear limbs, morphometrical type, sex, weight, and size. All the external measurements of the navicular bone decreased significantly with increasing age. From the histomorphometrical data, cortical bone volume decreased with age in most horses, whereas cancellous bone volume and, in particular, the marrow spaces increased. The increase in the cancellous bone volume could have resulted from tunnelling of the internal part of the cortex, which converted it progressively into a porous trabecular-like structure. Trabecular bone volume also decreased with age and the trabecular lattice changed dramatically to become disconnected in aged horses. These observations corresponded closely to those reported for ageing of the skeletal system in humans. However, in sporting horses, the navicular cortical bone volume increased with age and the cancellous bone volume decreased. Exercise appeared to have decreased bone resorption and increased bone formation at the endocortical junction. The cancellous bone architecture was also improved. in that the trabecular lattice and trabecular bone volume remained unchanged in aged sporting horses. Our findings confirmed that exercise may be good practice to prevent age-related bone loss. [less ▲]

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See detailCalculation Method for Design of Reinforced Concrete Columns under Fire Conditions
Dotreppe, Jean-Claude ULg; Franssen, Jean-Marc ULg; Vanderzeipen, Yves

in ACI Structural Journal (1999), 96(1), 9-18

This paper presents a simple model for the calculation of the fire resistance of reinforced concrete columns. It has been developed on the base of extensive numerical simulations and has been calibrated ... [more ▼]

This paper presents a simple model for the calculation of the fire resistance of reinforced concrete columns. It has been developed on the base of extensive numerical simulations and has been calibrated on the base of a large data base of full scale experimental test results [less ▲]

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See detailPreliminary classification of the bovine respiratory complex into different levels of severity
Coghe, Joost; Uystepruyst, Christophe; Bureau, Fabrice ULg et al

in Bovine Practitioner (1999), 33(1), 85-87

Despite prophylactic measures, mainly including vaccination programs and management measures, respiratory disease remains the major cause of economic losses in the bovine species. Choice of treatment is ... [more ▼]

Despite prophylactic measures, mainly including vaccination programs and management measures, respiratory disease remains the major cause of economic losses in the bovine species. Choice of treatment is currently mainly based on the clinicians' personal experience. A theoretical method has been proposed earlier to classify this syndrome into four grades of severity : Grade 1, subclinical disease (no treatment); Grade 2, compensated clinical disease (antibiotics only); Grade 3, noncompensated clinical disease (antibiotics + anti inflammatory drugs); and Grade 4, irreversible clinical disease (no treatment). In order to realize this theoretical classification under field conditions, three techniques, each one evaluating the oxygen transport chain at a différent level, have been studied and validated, i.e. pulse oximetry, lactate dosage and the lobeline test. Afterwards, measurements were performed on calves suffering from acute respiratory disease and a preliminary classification was established. This novel approach could orientate and economize therapeutic strategies in bovine respiratory disease. [less ▲]

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See detailWhen Drug Inactivation Renders the Target Irrelevant to Antibiotic Resistance: A Case Story with Beta-Lactams
Lakaye, Bernard ULg; Dubus, Alice ULg; Lepage, Sylvie ULg et al

in Molecular Microbiology (1999), 31(1), 89-101

By challenging the efficiency of some of our most useful antimicrobial weapons, bacterial antibiotic resistance is becoming an increasingly worrying clinical problem. A good antibiotic is expected to ... [more ▼]

By challenging the efficiency of some of our most useful antimicrobial weapons, bacterial antibiotic resistance is becoming an increasingly worrying clinical problem. A good antibiotic is expected to exhibit a high affinity for its target and to reach it rapidly, while escaping chemical modification by inactivating enzymes and elimination by efflux mechanisms. A study of the behaviour of a beta-lactamase-overproducing mutant of Enterobacter cloacae in the presence of several penicillins and cephalosporins showed that the minimum inhibitory concentration (MIC) values for several compounds were practically independent of the sensitivity of the target penicillin binding protein (PBP), even for poor beta-lactamase substrates. This apparent paradox was explained by analysing the equation that relates the antibiotic concentration in the periplasm to that in the external medium. Indeed, under conditions that are encountered frequently in clinical isolates, the factor characterizing the PBP sensitivity became negligible. The conclusions can be extended to all antibiotics that are sensitive to enzymatic inactivation and efflux mechanisms and must overcome permeability barriers. It would be a grave mistake to neglect these considerations in the design of future antibacterial chemotherapeutic agents. [less ▲]

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See detailSuccessful Treatment of Pulmonary Lymphangioleiomyomatosis with Progestins: A Case Report
Denoo, Xavier ULg; Hermans, Gilberte ULg; Degives, R. et al

in CHEST (1999), 115(1), 276-9

The diagnostic approach, clinical evolution, and treatment of a patient with primary pulmonary lymphangioleiomyomatosis are reported. This patient presented a restrictive respiratory syndrome resistant to ... [more ▼]

The diagnostic approach, clinical evolution, and treatment of a patient with primary pulmonary lymphangioleiomyomatosis are reported. This patient presented a restrictive respiratory syndrome resistant to conventional glucocorticoid therapy. The diagnosis, based on clinical and histologic examinations, was confirmed by immunohistochemical localization of one of the desmins, the smooth muscle cell actin, and HMB45 antigen. The patient received treatment with an anti-estrogenic agent (tamoxifen citrate) and high doses of medroxyprogesterone acetate, an antigonadotropic progestin. Respiratory function improved rapidly with clinical relief. [less ▲]

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See detailAnalysis of Endotoxin Effects on the Intact Pulmonary Circulation
Lambermont, Bernard ULg; Kolh, Philippe ULg; Detry, Olivier ULg et al

in Cardiovascular Research (1999), 41(1), 275-81

OBJECTIVE: The mechanism of sustained alterations in pulmonary hemodynamics during endotoxin shock remains unclear. To gain more detailed knowledge we used the four-element windkessel model as a ... [more ▼]

OBJECTIVE: The mechanism of sustained alterations in pulmonary hemodynamics during endotoxin shock remains unclear. To gain more detailed knowledge we used the four-element windkessel model as a descriptor of the pulmonary circuit. METHODS: Consecutive changes in characteristic resistance (R1), vascular compliance (C), input resistance (R2) and inductance (L) were continuously assessed following injection of endotoxin in 6 anaesthetised pigs, and were compared with the corresponding values measured in a similar group of sham-operated animals. RESULTS: Endotoxin challenge resulted in a biphasic pulmonary artery pressure response. Blood flow decreased progressively from 2.8 +/- 0.2 l/min to 2 +/- 0.2 l/min. Ohmic pulmonary vascular resistance (PVR) increased gradually from 0.2 +/- 0.04 to 0.76 +/- 0.1 mm Hg s ml-1. The early increase in PAP (from 14 +/- 2 to 27 +/- 4 mm Hg) was mediated by changes in both R1 (from 0.04 +/- 0.01 to 0.06 +/- 0.01 mm Hg s ml-1) and R2 (from 0.16 +/- 0.04 to 0.61 +/- 0.2 mm Hg s ml-1). These responses, in turn, altered the proximal vascular compliance. A subsequent increase in PAP (from 27 +/- 2 to 32 +/- 3 mm Hg) paralleled the specific decline in distal pulmonary vasculature compliance from 0.84 +/- 0.1 to 0.65 +/- 0.1 ml/mmHg. Analysis of the time course of PVR did not allow us to distinguish between vasoconstriction and stiffening of the vascular tree as mechanisms accounting for PAP changes. CONCLUSIONS: Endotoxemia leads to pulmonary hypertension, which is a result of constriction of proximal pulmonary arteries during the early phase, whereas the late phase is characterised by a decline in distal pulmonary vasculature compliance. [less ▲]

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See detailLes tumeurs malignes d'origine indéterminée
Rezaei Kalantari, Hassan ULg; Jerusalem, Guy ULg; Bours, Vincent ULg et al

in Revue Médicale de Liège (1999), 54(1), 39-42

Cancers of unknown origin form a specific clinical entity. The diagnostic procedure has to be guided by the therapeutical possibilities and requires a biopsy. Immunohistology, electron microscopy and ... [more ▼]

Cancers of unknown origin form a specific clinical entity. The diagnostic procedure has to be guided by the therapeutical possibilities and requires a biopsy. Immunohistology, electron microscopy and cytogenetics are informative for carcinomas and poorly differentiated adenocarcinomas. The clinical status and the histology allow the definition of groups of patients who can benefit from a specific therapy and have a better prognosis. For the other patients, the prognosis is severe and there is no efficient therapy available. Such tumors are usually characterized by a very aggressive behaviour. [less ▲]

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See detailWhole-Body 18f-Fdg Pet for the Evaluation of Patients with Hodgkin's Disease and Non-Hodgkin's Lymphoma
Jerusalem, Guy ULg; Warland, V.; Najjar, F. et al

in Nuclear Medicine Communications (1999), 20(1), 13-20

Whole-body metabolic information provided by 18F-FDG PET could help in the evaluation of lymphoma patients at diagnosis and follow-up. We studied 60 patients, 42 at initial presentation and 18 for disease ... [more ▼]

Whole-body metabolic information provided by 18F-FDG PET could help in the evaluation of lymphoma patients at diagnosis and follow-up. We studied 60 patients, 42 at initial presentation and 18 for disease recurrence (23 aggressive non-Hodgkin's lymphoma, 21 low-grade non-Hodgkin's lymphoma and 16 Hodgkin's disease). All patients underwent a clinical examination, computed tomography (CT) and a non-attenuated PET scan within 1 week. The patients received 222-296 MBq (6-8 mCi) 18F-FDG intravenously and emission scans were recorded 45-90 min later. 18F-FDG PET detected more lymph nodes than the clinical examination or CT, but this rarely resulted in upstaging (two patients). The concordance between PET and CT for the evaluation of the spleen, liver and digestive tract was quite good. Discordance was noted in 12 patients for the evaluation of bone marrow infiltration, but confirmation by MRI or focal biopsy was not always obtained. We conclude that non-attenuated 18F-FDG PET is an easy and efficient whole-body method for the evaluation of patients with lymphomas. Compared with conventional techniques, however, it does not appear to offer much improvement for staging but provides a satisfactory base for follow-up. [less ▲]

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See detailCardiovascular Response to Intravenous Administration of 5-Hydroxytryptamine after Type-2 Receptor Blockade, by Metrenperone, in Healthy Calves
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in Veterinary Journal (1999), 157(1), 31-37

The cardiovascular response to 5-hydroxytryptamine (5-HT) challenge has been previously described in cattle. Abrupt bradycardia, followed by tachycardia, triphasic systemic blood pressure response, and ... [more ▼]

The cardiovascular response to 5-hydroxytryptamine (5-HT) challenge has been previously described in cattle. Abrupt bradycardia, followed by tachycardia, triphasic systemic blood pressure response, and pulmonary hypertension were the major changes elicited by 5-HT. The purpose of the present study was to determine whether the cardiovascular response to 5-HT in calves was attributable to 5-HT2 receptors. A specific 5-HT2 antagonist (metrenperone, 0.05 mg/kg) was administered intramuscular to six unsedated Friesian calves 30 min before the animals were given a 5-min intravenous 5-HT infusion. Mean systemic arterial (SAP), mean pulmonary arterial (PAP), pulmonary capillary wedge (PW) pressures were obtained by means of fluid-filled catheters, and cardiac output (CO) was measured by the thermodilution technique. Heart rate, stroke volume, systemic (SVR) and pulmonary (PVR) vascular resistances were calculated. Administration of 5-HT after metrenperone induced a short-lasting period of severe bradycardia followed by tachycardia and increased CO. The systemic blood pressure response was exclusively hypotensive and associated with a decrease in SVR. Conversely, PAP, PW, and PVR were not modified by 5-HT administration. The results establish that 5-HT induced systemic as well as pulmonary hypertension is mediated through the activation of type-2 serotonergic or alpha-adrenergic receptors, or both. In contrast, neither apnoea, bradycardia and hypotension, nor the positive chronotropic effect induced by 5-HT in cattle are mediated through such receptors. [less ▲]

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See detailJames W. Carey, McLuhan : généalogie et descendance d'un paradigme
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999), 37

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See detailComment j'explore ... une pneumopathie interstitielle diffuse
Bury, Thierry ULg

in Revue Médicale de Liège (1999), 54(1), 54-7

There are a large number of conditions of known or unknown causes that have a tendency to alveolar wall fibrosis. The patient usually presents with progressive dyspnoea and the chest radiograph shows ... [more ▼]

There are a large number of conditions of known or unknown causes that have a tendency to alveolar wall fibrosis. The patient usually presents with progressive dyspnoea and the chest radiograph shows bilateral shadows whereas physiological measurements show a restrictive defect. Because of implications with regard to treatment and prognosis, diagnosis and staging of the disease are important. Computed tomography ("high resolution") and bronchoalveolar lavage are very useful at all stages of management of interstitial lung disease. [less ▲]

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See detailCardiac Troponin I and Troponin T: Recent Players in the Field of Myocardial Markers
Chapelle, Jean-Paul ULg

in Clinical Chemistry & Laboratory Medicine (1999), 37(1), 11-20

The troponin (Tn) complex consists of three subunits referred to as TnT, TnI and TnC. Myocardium contains TnT and TnI isoforms which are not present in skeletal muscles and which can be separated from the ... [more ▼]

The troponin (Tn) complex consists of three subunits referred to as TnT, TnI and TnC. Myocardium contains TnT and TnI isoforms which are not present in skeletal muscles and which can be separated from the muscular isoforms by immunological techniques. Using commercially available immunoassays, clinical laboratories are able to determine cardiac TnT and TnI (cTnT and cTnI) quickly and reliably as classical cardiac markers. After acute myocardial infarction, cTnT and cTnI concentrations start to increase in serum in a rather similar way than CK-MB, but return to normal after longer periods of time (approximately one week). Because of their excellent cardiac specificity, Tn subunits appear ideally suited for the differential diagnosis of myocardial and muscular damage, for example in noncardiac surgery patients, in patients with muscular trauma or with chronic muscular diseases, or after intense physical exercise. cTnT and cTnI may also be used for detecting evidence of minor myocardial damage: therefore they have found new clinical applications, in particular risk stratification in patients with unstable angina. In spite of the possible reexpression of cTnT in human skeletal muscles, and of the lack of standardization of cTnI assays, Tn subunits are not far to meet the criteria of ideal markers for acute myocardial injury. Only an insufficient sensitivity in the first hours following the acute coronary syndroms requiries to maintain an early myocardial marker in the cardiac panel for routine laboratory testing. [less ▲]

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See detailRuth & Elihu Katz, D'où venait-il, où a-t-il disparu ?
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999), 37

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See detailL'image du mois. Kyste hydatique hépatique
Radermecker, Régis ULg; Nkoghe, D.; Labasse, J. M. et al

in Revue Médicale de Liège (1999), 54(1), 4-5

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See detailThe ribonucleoside diphosphate reductase inhibitor (E)-2'-Deoxy-(fluoromethylene) cytidine, acts as a cytotoxic radiosensitizer on human cancer cell lines in vitro.
Coucke, Philippe ULg; Decosterd, L-A; Li, Y-X et al

in Cancer Research (1999), 59

ABSTRACT (E)-2*-Deoxy-(fluoromethylene)cytidine (FMdC) is known as an inhibitor of ribonucleoside diphosphate reductase, a key enzyme in the de novo pathway of DNA synthesis. FMdC was tested as a modifier ... [more ▼]

ABSTRACT (E)-2*-Deoxy-(fluoromethylene)cytidine (FMdC) is known as an inhibitor of ribonucleoside diphosphate reductase, a key enzyme in the de novo pathway of DNA synthesis. FMdC was tested as a modifier of radiation response in vitro on a human colon carcinoma cell line (WiDr), and the observed radiosensitization was confirmed on two human cervix cancer cell lines (C33-A and SiHa). Using the clonogenic assay, the effect ratio (ER) at a clinically relevant dose level of 2 Gy was 2.10 (50 nM FMdC), 1.70 (30 nM FMdC), and 1.71 (40 nM FMdC) for the three cell lines WiDr, C33-A, and SiHa, respectively. A more detailed analysis of the importance of timing and concentration of FMdC was done on the WiDr cell line alone, yielding an increased ER(2Gy) with increasing concentration and duration of exposure to the drug, ranging from 1.0 (6 h) to 1.8 (72 h) at 30 nM FMdC and from 1.2 (6 h) to 3.5 (24 h) at 300 nM. We investigated the effect of FMdC on the cellular deoxynucleotide triphosphate pool in WiDr cells and demonstrated a marked depletion of dATP and a significant rise of TTP levels. Cell cycle analysis showed early S-phase accumulation induced by FMdC alone, G2-M block induced by irradiation alone, and an increased accumulation of cells in G2-M if both modalities are used. Our data suggest that FMdC is a radiation response modifier in vitro on different cancer cell lines. The observed radiosensitization may in part be explained by alteration of the deoxynucleotide triphosphate pool, which is consistent with the effect of FMdC on ribonucleoside diphosphate reductase. [less ▲]

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See detailDevelopment of a new hydrocarbon index for oil-in-water
Kvernheim, A. L.; Lund, H. S.; Berg, B. et al

in Chemosphere (1999), 39(15), 2707-2722

A new robust method for determination of hydrocarbons in water without use of Freon(TM) or other halogenated solvents has been validated and subjected to an interlaboratory exercise. The method is based ... [more ▼]

A new robust method for determination of hydrocarbons in water without use of Freon(TM) or other halogenated solvents has been validated and subjected to an interlaboratory exercise. The method is based on extraction with a light hydrocarbon (boiling point in the 39-69 degrees C range), followed by cleanup for removal of polar components and detection by gas chromatography (GC) with flame ionisation (FID) detection. The performance characteristics of the method are comparable with that of the previous Freon-IR method with recoveries in the 60-80% range and reproducibility (between laboratory variations) of about 30%. A screening method for hydrocarbons in the range 5 ppm and above based on infrared spectroscopy (IR), and a promising method for detection of low levels based on solid phase extraction (SPE) has been tested and validated. (C) 1999 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailUltrastructural methods for nucleic acid detection by immunocytology.
Thiry, Marc ULg

in Progress in Histochemistry and Cytochemistry (1999), 34(2), 87-159

In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to ... [more ▼]

In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to natural nucleic acids are described but also the techniques involving the use of antibodies raised against various nucleotide analogs incorporated beforehand into nucleic acids. Special emphasis is placed on each method's potential and limitations. These methods, combined or not with molecular biotechnology, are powerful tools for studying the structure and function of nucleic acids. They can be used to investigate the distribution and topological organization of DNA and RNA molecules or of specialized within these molecules in the cells. [less ▲]

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See detailA Basis for Using Fire Modeling with 1-D Thermal Analyses of Partitions to Simulate 2-D and 3-D Structural Performance in Real Fires
Cooper, Len; Franssen, Jean-Marc ULg

in Fire Safety Journal (1999), 33

A basis is presented for identifying partition design for which: 1) the use of one-dimensional thermal analysis in the fire modeling part of the problem would lead to reliable simulations of the fire ... [more ▼]

A basis is presented for identifying partition design for which: 1) the use of one-dimensional thermal analysis in the fire modeling part of the problem would lead to reliable simulations of the fire environment, and 2) the resulting simulations can be used to provide the necessary boundary conditions input to solve the thermal/structural part of the problem, using two- or three-dimensional analyses, as required. [less ▲]

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See detailHypoxia-induced activation of HIF-1: role of HIF-1alpha-Hsp90 interaction.
Minet, E.; Mottet, Denis ULg; Michel, G. et al

in FEBS Letters (1999), 460(2), 251-6

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear ... [more ▼]

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear translocator (ARNT)-Sim (PAS) factors Sim and aryl hydrocarbon receptor (Ahr). The transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha), involved in the response to hypoxia, also belongs to the bHLH-PAS family. This work was aimed to investigate the putative role of Hsp90 in HIF-1 activation by hypoxia. Using a EGFP-HIF-1alpha fusion protein, co-immunoprecipitation experiments evidenced that the chimeric protein expressed in COS-7 cells interacts with Hsp90 in normoxia but not in hypoxia. We also demonstrated that Hsp90 interacts with the bHLH-PAS domain of HIF-1alpha. Moreover, Hsp90 is not co-translocated with HIF-1alpha into the nucleus. At last, we showed that Hsp90 activity is essential for HIF-1 activation in hypoxia since it is inhibited in the presence of geldanamycin. These results indicate that Hsp90 is a major regulator in HIF-1alpha activation. [less ▲]

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See detailComparaison des Kess-Kess de Hmar Lakhdad (Emsien, Maroc) et des monticules micritiques de l'anticlinorium de Philippeville (Frasnien, Belgique)
Hilali, A.; Lachkem, H.; Boulvain, Frédéric ULg

in Geologica Belgica (1999), 1

A Comparison between the Kess-kess of Hmar Lakhdad (Emsian, Morocco) and the mud mounds of the Philippeville Anticlinorium (Frasnian, Belgium). The Kess-kess of Hmar Lakhdad are Devonian mud mounds ... [more ▼]

A Comparison between the Kess-kess of Hmar Lakhdad (Emsian, Morocco) and the mud mounds of the Philippeville Anticlinorium (Frasnian, Belgium). The Kess-kess of Hmar Lakhdad are Devonian mud mounds, located SE of Erfoud (Moroccan Antiatlas). Fenestral structures, which are very abundant, occur from the base to the top of most buildups. These structures are classified in two groups: stromatactis s.s. and stromatactoid fenestrae. each of them showing a high morphological variability. These fenestrae look very similar to the structures already described from the Belgian mud mounds. However, their attribution to sponges cannot be demonstrated. Microstromatolifhes, a microbial structure, are common in the Moroccan and Belgian buildups. The numerous sedi-mentological similarities between the two types of mounds allow to assign the facies of the Kess-kess to the coral-crinoidal limestones at the base of the Belgian buildups. This comparison justifies a paleoecological and paleobathy-metrical interpretation of the Moroccan mud mounds. [less ▲]

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See detailKarst dans les évaporites : marqueur efficace des événements récents. L’exemple du Mont Sédom (Israel)
Hallot, Eric ULg

in Bulletin de la Société Géographique de Liège (1999), 37

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See detailAre The Fusion Processes Involved In Birth, Life And Death Of The Cell Depending On Tilted Insertion Of Peptides Into Membranes?
Peuvot, J.; Schanck, A.; Lins, Laurence ULg et al

in Journal of Theoretical Biology (1999), 198(2), 173-81

Various peptide segments have been modeled as asymmetric amphipathic alpha-helices. Theoretical calculations have shown that they insert obliquely into model membranes. They have been named "tilted ... [more ▼]

Various peptide segments have been modeled as asymmetric amphipathic alpha-helices. Theoretical calculations have shown that they insert obliquely into model membranes. They have been named "tilted peptides". Molecular modeling results reported here also evidence the presence of tilted peptides in ADM-1 protein of Caenorhabditis elegans that may be involved in fusion events, in meltrin alpha, a protein implicated in myoblast fusion, in hemagglutinin of influenza virus, in the E2 glycoprotein of rubella virus, in the S protein of hepatitis B virus, in a subdomain of Ebola virus and in the malaria CS protein. Experimental results have indicated that tilted peptide fragments may be involved in cellular life events like sperm-egg fecondation, muscle development, protein translocation through signal sequences and cellular death caused by viral infection or parasite infestation. We speculate that membrane destabilization by these tilted peptides may be an important common step in life processes involving fusion phenomena. [less ▲]

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See detailNew evaluation therapies for sepsis.
Deby; Damas, Pierre ULg; Lamy, Maurice ULg

in Current Opinion in Anaesthesiology (1999), 12(2), 121-7

This article presents an analysis of the literature of the last 2 years on the treatment of sepsis and septic shock. It appears that most of the antimediator therapies applied in phase II or III clinical ... [more ▼]

This article presents an analysis of the literature of the last 2 years on the treatment of sepsis and septic shock. It appears that most of the antimediator therapies applied in phase II or III clinical trials yielded disappointing results. But, many other therapeutic approaches are proposed or are ongoing, and many papers report critical analysis of the reasons for the lack of success of past clinical trials. [less ▲]

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See detailHistidine modification and mutagenesis point to the involvement of a large conformational change in the mechanism of action of phage lambda lysozyme
Evrard, Christine ULg; Fastrez, Jacques; Soumillion, Patrice

in FEBS Letters (1999), 460

Phage lambda lysozyme is structurally related to other known lysozymes but its mechanism of action is different from the classical lysozyme mechanism, acting as a transglycosidase rather than a hydrolase ... [more ▼]

Phage lambda lysozyme is structurally related to other known lysozymes but its mechanism of action is different from the classical lysozyme mechanism, acting as a transglycosidase rather than a hydrolase. As two conformations have been revealed by the crystal structure, we investigated the effect of mutating and modifying a histidine located near to or far from the active site in the respective closed and open conformations. Whereas its asparagine mutation has little or no effect on activity, its N-carbethoxylation inactivates the enzyme. This provide further evidence for the involvement of the closed conformation and for the need of conformational mobility in lambda lysozyme function. [less ▲]

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See detailLes etudes cliniques controlees, support incontournable de la medecine basee sur l'evidence.
Scheen, André ULg; Kulbertus, Henri ULg

in Revue Médicale de Liège (1999), 54(1), 1

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See detailIntérêt de la tomographie à émission de positons dans l'évaluation des tumeurs gastro-intestinales
Rigo, Pierre ULg; Albérini, Jean-Louis; Hustinx, Roland ULg et al

in Acta Endoscopica (1999), 29(2), 129-138

La TEP au 18FDG présente de nombreuses indications dans l'évaluation des tumeurs digestives. Son rôle principal concerne le bilan d'extension des récidives tumorales démontrées ou suspectées mais des ... [more ▼]

La TEP au 18FDG présente de nombreuses indications dans l'évaluation des tumeurs digestives. Son rôle principal concerne le bilan d'extension des récidives tumorales démontrées ou suspectées mais des indications plus ponctuelles concernent évalement le diagnostic différentiel des masses pancréatiques et le bilan du cancer de l'oesophage. Le principal avantage de la TEP résulte de la nature métabolique du signal indépendant et complémentaire des modifications anatomiques visibles en imagerie classique. Un autre avantage est lié à l'examen du corps entier aujourd'hui pratiqué systématiquement. La TEP trouve dès lors sa place en première ligne dans ses différentes indications. [less ▲]

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See detailSur philellên chez Cicéron
Rochette, Bruno ULg

in Antiquité Classique : Revue Interuniversitaire d'Etudes Classiques (1999), 68

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See detailTransplantation combinée du foie et du coeur chez un patient souffrant de thalassémie majeure
Detry, Olivier ULg; Defechereux, Thierry ULg; Honore, Pierre ULg et al

in Médecine & Chirurgie Digestives (1999), 28(3), 109-110

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See detailMimicry and the judgment of emotional facial expression
Blairy, Sylvie ULg; Herrera, P; Hess, U

in Journal of Nonverbal Behavior (1999), 23(1), 5-41

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See detailHarm avoidance and serotonin.
Hansenne, Michel ULg; Ansseau, Marc ULg

in Biological Psychology (1999), 51(1), 77-81

The relationships between the Tridimensional Personality Questionnaire (TPQ) and serotonergic activity has been described in some studies with controversial results. These studies have focused on specific ... [more ▼]

The relationships between the Tridimensional Personality Questionnaire (TPQ) and serotonergic activity has been described in some studies with controversial results. These studies have focused on specific patient populations rather than normal controls. Therefore, the aim of the present study is to examine the relationships between the TPQ and serotonergic activity in a group of non-patient subjects. Twenty-three normal subjects answered the TPQ, and the serotonergic activity was assessed by the prolactin response to a highly potent and selective 5-HT1a agonist (flesinoxan). A positive relationship between harm avoidance and PRL response to flesinoxan was found. This study is consistent with the hypothesized link between serotonergic activity and the harm avoidance dimension of the biosocial model of Cloninger. [less ▲]

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See detailThe Crystal Structure Of A Penicilloyl-Serine Transferase Of Intermediate Penicillin Sensitivity - The Dd-Transpeptidase Of Streptomyces K15
Fonze, E.; Vermeire, M.; Nguyen-Disteche, M. et al

in Journal of Biological Chemistry (1999), 274(31), 21853-60

The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide bond formation in a way that mimics the penicillin-sensitive peptide cross-linking reaction involved in ... [more ▼]

The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide bond formation in a way that mimics the penicillin-sensitive peptide cross-linking reaction involved in bacterial cell wall peptidoglycan assembly. The Streptomyces K15 enzyme is peculiar in that it can be considered as an intermediate between classical penicillin-binding proteins, for which benzylpenicillin is a very efficient inactivator, and the resistant penicillin-binding proteins that have a low penicillin affinity. With its moderate penicillin sensitivity, the Streptomyces K15 DD-transpeptidase would be helpful in the understanding of the structure-activity relationship of this penicillin-recognizing protein superfamily. The structure of the Streptomyces K15 enzyme has been determined by x-ray crystallography at 2.0-A resolution and refined to an R-factor of 18.6%. The fold adopted by this 262-amino acid polypeptide generates a two-domain structure that is close to those of class A beta-lactamases. However, the Streptomyces K15 enzyme has two particular structural features. It lacks the amino-terminal alpha-helix found in the other penicilloyl-serine transferases, and it exhibits, at its surface, an additional four-stranded beta-sheet. These two characteristics might serve to anchor the enzyme in the plasma membrane. The overall topology of the catalytic pocket of the Streptomyces K15 enzyme is also comparable to that of the class A beta-lactamases, except that the Omega-loop, which bears the essential catalytic Glu(166) residue in the class A beta-lactamases, is entirely modified. This loop adopts a conformation similar to those found in the Streptomyces R61 DD-carboxypeptidase and class C beta-lactamases, with no equivalent acidic residue. [less ▲]

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See detailL'etude clinique du mois. Interet d'augmenter un taux de cholesterol HDL bas en prevention secondaire de la coronaropathie: resultats de l'etude VA-HIT.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(9), 773-5

The "Veterans Affairs Cooperative Studies Program High-Density Lipoprotein Cholesterol Intervention Trial" (VA-HIT) is a large randomised, double-blind, placebo-controlled clinical trial for the secondary ... [more ▼]

The "Veterans Affairs Cooperative Studies Program High-Density Lipoprotein Cholesterol Intervention Trial" (VA-HIT) is a large randomised, double-blind, placebo-controlled clinical trial for the secondary prevention of coronary heart disease. It demonstrates that a fibrate treatment (gemfibrozil) significantly reduces the relative risk of major coronary (-22%, p = 0.006) and cardiovascular (-24%, p < 0.001) events in men with coronary heart disease whose primary lipid abnormality is a low HDL cholesterol level. These findings suggest that, in such a population, the rate of coronary events is reduced under gemfibrozil therapy by raising HDL cholesterol levels (+6%) and lowering levels of triglycerides (-31%) without lowering LDL cholesterol concentrations. [less ▲]

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See detailLe trésor des reliques de l'abbaye Saint-Hubert en Ardenne
George, Philippe ULg

in Études & Documents de la Région Wallonne. Monuments & Sites (1999)

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See detailCandidate gene markers associated with somatotropic axis and milk selection
Parmentier, I.; Portetelle, Daniel ULg; Gengler, Nicolas ULg et al

in Domestic Animal Endocrinology (1999), 17(2-3), 139-148

One of the obstacles to progress in dairy cattle selection is that milk production traits are only expressed after the first calving. However, the use of the quantitative trait loci (QTL) technology will ... [more ▼]

One of the obstacles to progress in dairy cattle selection is that milk production traits are only expressed after the first calving. However, the use of the quantitative trait loci (QTL) technology will improve the efficiency of dairy industry with a positive image for the consumers. QTL are part of the genome showing a preponderant action and explaining the major part of variation of the trait production. At the present time, the two major strategies developed to detect such QTL are the candidate gene approach and the positional genetics approach. The somatotropic axis contains the most promising candidates in this respect, as it strongly regulates milk production. Then, the identification of favorable QTL associated with the somatotropic axis that are significantly correlated with genetic merits for milk production could lead to more effective selection programs. (C) 1999 Elsevier Science Inc. All rights reserved. [less ▲]

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See detailA procedure for deriving accurate linear polarimetric measurements.
Lamy, H.; Hutsemekers, Damien ULg

in The Messenger (1999), 96

The authors present here a procedure written within the ESO MIDAS reduction package with the aim of deriving semi-automatically linear polarisation data from CCD images obtained with beam-splitters such ... [more ▼]

The authors present here a procedure written within the ESO MIDAS reduction package with the aim of deriving semi-automatically linear polarisation data from CCD images obtained with beam-splitters such as those available at the ESO 3.6-m telescope equipped with EFOSC2 or at the VLT equipped with FORS1. The method is adequate for point-like objects and was used for measuring quasar polarisation. [less ▲]

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See detailConversion of green note aldehydes into alcohols by yeast alcohol dehydrogenase
Fauconnier, Marie-Laure ULg; Mpambara, A.; Delcarte, Jérôme et al

in Biotechnology Letters (1999), 21(7), 629-633

‘Green note’ aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into ... [more ▼]

‘Green note’ aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into (Z)-3-hexenol, Pichia anomala gave the best results. Conversion yields higher than 90% were also obtained by directly conducting the reaction in the medium where (Z)-3-hexenal is produced by the action of lipoxygenase and hydroperoxide lyase on <br />linolenic acid. [less ▲]

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See detailRed blood cell substitutes: fluorocarbon emulsions and haemoglobin solutions.
Remy, Bernadette ULg; Deby, Ginette ULg; Lamy, Maurice ULg

in British Medical Bulletin (1999), 55(1), 277-98

The problems posed by transfusion of homologous blood have led to the development of substances able to replace the gas transporting properties of blood. Perfluorocarbons (PFCs) emulsions and modified ... [more ▼]

The problems posed by transfusion of homologous blood have led to the development of substances able to replace the gas transporting properties of blood. Perfluorocarbons (PFCs) emulsions and modified haemoglobin (Hb) solutions have been developed for this goal and are now tested in clinical assays. PFCs are synthetic fluorinated hydrocarbons, capable of dissolving large quantities of oxygen (O2; without binding) at high inspired concentrations of O2, and of delivering this O2 to the tissues. They are administered as emulsions containing particles with a diameter of approximately 0.2 micron, capable of entering the microcirculation. They are eliminated unchanged by the lungs within several days. Fluosol-DA 20% was the first PFC emulsion used in clinical practice. Currently, Oxygent, a second generation PFC emulsion, is being evaluated in clinical studies. The PFCs are not blood substitutes, but rather a means to ensure tissue oxygenation during extreme haemodilution. Solutions of free Hb do not have the antigenic characteristics of the blood groups, and do not require compatibility testing. They are fully saturated with O2 at ambient FiO2. The Hbs used are derived from either human or bovine sources, or via recombinant DNA technology. In order to maintain satisfactory intravascular half-life and O2 affinity, the Hb molecules are modified by adding internal crosslinks, by polymerization, and/or by encapsulation. After promising animal studies, several of these modified Hb solutions are now being studied in Phase III clinical trials. Among them, diaspirin cross-linked haemoglobin (DCLHb) has been used in cardiac and orthopaedic surgery, and for resuscitation of traffic accident victims. The initial results of multicentre trials are now being analysed. [less ▲]

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See detailRecombinant Production Of The P10(Cks1at) Protein From Arabidopsis Thaliana And C-13 And N-15 Double-Isotopic Enrichment For Nmr Studies
Landrieu, I.; Casteels, P.; Odaert, B. et al

in Protein Expression & Purification (1999), 16(1),

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See detailEffects of inhaled dry powderipratropium broide on reconvery from exercise of horses with COPD
Duvivier, Dominique Hannia; Bayly, Warwick M; Votion, Dominique ULg et al

in Equine Veterinary Journal (1999), 31(1), 20-24

The present study evaluated ventilatory, cardiovascular and metabolic parameters during recovery from strenuous exercise in horses suffering from a crisis of chronic obstructive pulmonary disease (COPD ... [more ▼]

The present study evaluated ventilatory, cardiovascular and metabolic parameters during recovery from strenuous exercise in horses suffering from a crisis of chronic obstructive pulmonary disease (COPD) and to determine whether ipratropium dry powder inhalation (DPI) before exercise has an effect on these parameters. When 6 saddle horses, affected with COPD, developed airway obstruction, they inhaled placebo and ipratropium (2400 pghorse), the order being randomly chosen. Pulmonary function tests were then recorded 15 min after inhalation. Following these tests, the horses underwent a strenuous treadmill exercise, followed by a ecovery period that consisted of a 10 min walk. Measurements were made at the first and tenth min of recovery. Respiratory flow, 0 2 and C02 fractions in the respired gas, pleural pressure changes and heart rate were recorded. Arterial and mixed venous blood samples were analysed or gas tensions, haemoglobin and plasma lactate concentrations. Oxygen consumption (Wz), C02 production, tidal volume, alveolar oxygen tension (PAOz), alveolar ventilation, the alveolar-pulmonary capillary oxygen difference ((A-a)dOz) and total pulmonary resistance (RL) were measured. The PA02 was the only parameter significantly improved during recovery following ipratropium DPI. This improvement was not accompanied by evidence of improvement of other ventilatory or cardiorespiratory parameters. The results showed that in horses suffering from a crisis of COPD, recovery is characterised by an exercise-induced bronchodilation. Secondly, ipratropium DPI at a dose of 2400 pghorse is an effective bronchoddator in these horses at rest but it has little effect on the airway calibre during the recovery period. It is suggested that the short term recovery period is still Muenced by exercise-induced adjustments that may exceed the bronchodilatory effect of inhaled ipratropium that are observed before exercise [less ▲]

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See detailDevelopment of a coupled flow and transport 3D model for simulating seawater intrusions in coastal aquifers
Carabin, Guy; Dassargues, Alain ULg

in Natuurwetenschappelijk Tijdschrift (1999), 79

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See detailIntroduction
Martiniello, Marco ULg; Statham, Paul

in Journal of Ethnic & Migration Studies (1999), 25(4), 565-573

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See detailLes adénovirus recombinants comme vecteurs vaccinaux
Gogev, S.; Thiry, Etienne ULg

in Annales de Médecine Vétérinaire (1999), 143

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See detailReproducibility of measurements of potential doubling time of tumour cells in the multicentre National Cancer Institute protocol
COUCKE, Philippe ULg; Paschoud, N; Pavy, J-J et al

in British Journal of Cancer (1999)

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose ... [more ▼]

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose was to understand and minimize the variation within the measurement. A total of 102 specimens were selected at random from patients entered into the trial. Samples were prepared, stained, run and analysed in each centre and a single set of data analysed by all three centres. Analysis of the disc data set revealed that the measurement of labelling index (LI) was robust and reproducible. The estimation of duration of S-phase ( Ts) was subject to errors of profile interpretation, particularly DNA ploidy status, and analysis. The LI dominated the variation in Tpot such that the level of final agreement, after removal of outliers and ploidy agreement, reached correlation coefficients of 0.9. The sample data showed poor agreement within each of the components of the measurement. There was some improvement when ploidy was in agreement, but correlation coefficients failed to exceed values of 0.5 for Tpot. The data suggest that observer-associated analysis of Ts and tissue processing and tumour heterogeneity were the major causes of variability in the Tpot measurement. The first two aspects can be standardized and minimized, but heterogeneity will remain a problem with biopsy techniques. [less ▲]

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See detailHemodynamic changes and catecholamine release during laparoscopic adrenalectomy for pheochromocytoma.
Joris, Jean ULg; Hamoir, Etienne ULg; Hartstein, Gary ULg et al

in Anesthesia and Analgesia (1999), 88(1), 16-21

We investigated hemodynamics and plasma catecholamine concentrations in eight consecutive patients undergoing laparoscopic adrenalectomy for suspected pheochromocytoma. The same anesthesia protocol was ... [more ▼]

We investigated hemodynamics and plasma catecholamine concentrations in eight consecutive patients undergoing laparoscopic adrenalectomy for suspected pheochromocytoma. The same anesthesia protocol was used in all patients: a continuous infusion of sufentanil 0.5 microg x kg(-1) x h(-1) and isoflurane 0.4% (end-tidal) in 50% N2O/O2. Systolic arterial pressure was maintained between 120 and 160 mm Hg by adjusting an infusion of nicardipine, a calcium-channel blocker, while tachycardia (>100 bpm) was treated by 1-mg boluses of atenolol. Hemodynamics (thermodilution technique) and plasma catecholamine concentrations were measured before surgery, after the induction of anesthesia, after turning the patient to the lateral position, during pneumoperitoneum, during tumor manipulation, after adrenalectomy, and at the end of surgery. Two events resulted in significant catecholamine release: creation of the pneumoperitoneum and adrenal gland manipulation. As a consequence, a twofold increase in cardiac output was recorded. Adjustments of nicardipine infusion (2-6 microg x kg(-1) x min(-1)) minimized changes in mean arterial pressure. Beta-adrenergic blockade was necessary in six patients. In conclusion, laparoscopic adrenalectomy for pheochromocytoma results in marked catecholamine release during pneumoperitoneum and tumor manipulation. Titration of a nicardipine infusion allowed easy and quick control of the hemodynamic aberrancies related to these processes. IMPLICATIONS: Pneumoperitoneum during laparoscopy, now used for adrenalectomy, may complicate anesthetic management of patients with pheochromocytoma. In this study, laparoscopic adrenalectomy was associated with catecholamine release during the creation of pneumoperitoneum and tumor manipulation. Adjustments of a nicardipine infusion readily attenuated the subsequent hemodynamic aberrancies. [less ▲]

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See detailLes Caecilii, la Sicile et le judaïsme
Rochette, Bruno ULg

in Maia (1999), 51

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See detailMise au point de pains composites à base de mélanges de farines de sorgho/blé et analyse texturale.
Balla, A.; Blecker, Christophe ULg; Oumarou, M. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(2), 69-77

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See detailThe structure of two fengycins from Bacillus subtilis S499.
Schneider, J.; Taraz, K.; Budzikiewicz, H. et al

in Zeitschrift für Naturforschung. C, Journal of Biosciences (1999), 54(11), 859-66

The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins ... [more ▼]

The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins from Bacillus cereus differing only in the stereochemistry of the Tyr residues. [less ▲]

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See detailDo leaching experiments affect the Sm-Nd signature of deep-sea sediments? The example of the northern North Atlantic
Innocent, Christophe; Fagel, Nathalie ULg; Stevenson, Ross

in Canadian Journal of Soil Science (1999), 79

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See detailFar-field decoupled basis for the method of moments-2D case
V Sabariego, Ruth ULg; Landesa, Luis; Obelleiro, Fernando

in Progress in Electromagnetics Research [=PIER] (1999), 24

When solving electromagnetic problems, integral equation methods, such as the Method of Moments, are known to yield to dense matrix systems whose solution usually supposes an intensive computational cost ... [more ▼]

When solving electromagnetic problems, integral equation methods, such as the Method of Moments, are known to yield to dense matrix systems whose solution usually supposes an intensive computational cost. Continuous efforts have been developed to overcome this drawback. In this paper, the goal is to generate a set of basis functions defined over the entire domain and characterized by a null radiated power coupling in the far-field region. This behavior permits to diminish the density of unknowns per wavelength, considering only a reduced set of the new basis. Furthermore, the resulting matrix has a sparse structure. All these factors amounts to considerable savings in terms of computer storage and CPU requirements. [less ▲]

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See detailA critical compilation and extended analysis of the Ne V spectrum
Kramida, A. E.; Bastin, Thierry ULg; Biémont, Emile ULg et al

in European Physical Journal D -- Atoms, Molecules, Clusters & Optical Physics (1999), 7

Previous works devoted to the Ne V spectrum are very fragmentary and yield a rather controversial picture of this ion. A lot of unidentifi ed lines are still reported in these multiple studies. In the ... [more ▼]

Previous works devoted to the Ne V spectrum are very fragmentary and yield a rather controversial picture of this ion. A lot of unidentifi ed lines are still reported in these multiple studies. In the present paper, Hartree-Fock calculations and least-squares fi ts over the whole set of the available data (including the most recent ones) have been performed. As a result, a comprehensive view of the Ne V spectrum is presented. 10 new energy levels and 40 newly classi fied lines are proposed. An improved value of the ionisation potential of Ne V is also proposed. [less ▲]

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