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See detailVector mesons and partial restoration of chiral symmetry
Mendez Galain, Ramon; Ripka, Georges; Jaminon, Martine ULg et al

in Europhysics Letters [=EPL] (1991), 14

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See detailLa topoclimatologie, un outil au service de l'agronomie. Exemples pris en Belgique
Erpicum, Michel ULg

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(1)

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See detailTransient T and B cell activation after neonatal induction of tolerance to MHC class II and Mls alloantigens
Schurmans, Stéphane ULg; Brighouse, G.; Kramar, G. et al

in Journal of Immunology (1991), 146

The neonatal injection of semiallogeneic F1 spleen cells into newborn parental mice results in the induction of tolerance to the corresponding alloantigen (alloAg) and chimerism. In these F1 cell-injected ... [more ▼]

The neonatal injection of semiallogeneic F1 spleen cells into newborn parental mice results in the induction of tolerance to the corresponding alloantigen (alloAg) and chimerism. In these F1 cell-injected mice, we have previously observed that this state of specific tolerance is associated with the development of a transient lupus-like autoimmune syndrome. In this study, we show that neonatal injection of mice with spleen cells differing from the host at major histocompatibility complex (MHC) class I, class II, class (I + II), or minor lymphocyte stimulating (Mls) alloAg induced a state of specific tolerance characterized by the absence of alloreactive CTL and/or Th cell responses in the spleen and the thymus of 6- to 12-week-old injected mice. However, in mice rendered tolerant to MHC class II or class (I + II) alloAg, the presence of high levels of IgG1 antibodies, of circulating immune complexes, of anti-ssDNA autoantibodies, and of tissue lesions were transiently observed. In these mice, an increased Ia Ag expression on lymphoid spleen cells was also detected at 1 wk. The elevated production of IgG1 and the overexpression of Ia Ag were almost completely prevented by treatment with an anti-IL-4 mAb. Such manifestations of B cell activation and autoimmunity were not observed in mice neonatally injected with F1 cells differing from the host only at MHC class I Ag. In mice neonatally tolerized to Mls Ag, a transient increase in IgG2a production and an overexpression of Ia Ag were detected without features of autoimmunity, and were prevented by anti-INF-gamma mAb treatment. In mice rendered tolerant to MHC class II, class (I + II), or Mls alloAg at birth, the manifestations of B cell activation were associated with the presence of in vivo-activated alloreactive CD4+ T cells in the spleen--but not the thymus--of 1-wk-old injected mice. Together, these results suggest that in mice neonatally injected with semiallogeneic F1 cells, the process of tolerance induction is not efficient during the early postnatal period, and could allow the maturation and peripheralization of some alloreactive CD4+ T cells, leading to transient B cell activation and, depending on the alloAg, to autoimmunity [less ▲]

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See detailAbnormal gene expression in skin fibroblasts from a Hutchinson-Gilford patient.
Colige, Alain ULg; Roujeau, J. C.; De la Rocque, F. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1991), 64(6), 799-806

We had the opportunity to investigate a new case of Hutchinson-Gilford progeria, a rare disease commonly regarded as a model in the study of aging. Two strains of fibroblasts (strains 1 and 2) were ... [more ▼]

We had the opportunity to investigate a new case of Hutchinson-Gilford progeria, a rare disease commonly regarded as a model in the study of aging. Two strains of fibroblasts (strains 1 and 2) were derived from two pieces of a skin biopsy. These two populations multiplied as normal cells at low population doubling level but senesced rapidly and stopped proliferating after 14 or 15 population doubling levels. Interestingly, an unusual pattern of growth in clusters was observed for strain 1. The level of collagen and noncollagen protein synthesis of both strains of affected fibroblasts was similar to that of normal fibroblasts as determined by [3H]proline incorporation measurement and was similarly affected by varying serum concentrations. The pattern of the main types of newly synthesized collagen polypeptides analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was similar in normal and progeria cells. The steady-state level of mRNAs coding for macromolecules of the extracellular matrix did not provide any differences between affected and control fibroblasts except for a strong increase of elastin and of alpha 1 and alpha 2 type IV procollagen mRNA mainly in strain 1 and less marked in strain 2. Interestingly, senescent progeria fibroblasts exhibited a reduced level of all the tested mRNAs, whereas collagen type IV and elastin mRNAs remained elevated. As suggested by immunofluorescence and immunoblotting studies, the increased amount of type IV mRNAs was paralleled by an enhanced production of type IV collagen by fibroblasts in vitro. Histologic examination of the skin revealed a superabundant network of abnormal elastic fibers in the reticular dermis and a thickening of basement membranes. The relationship between these alterations and aging in progeria is discussed. [less ▲]

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See detailIntroduction à l'arithmétique des algèbres de quaternions
Pays, Isabelle ULg; Tignol, Jean-Pierre

in Note di Matematica e Fisica, Cerfim, Locarno (5) (1991)

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See detailEvidence of West European defined miospore zones in the Uppermost Devonian and Lower Carboniferous of the Amazonas Basin (Brazil).
Loboziak, S; Streel, Maurice ULg; Caputo, M.V. et al

in Geobios (1991), 24(1), 5-11

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See detailArrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
Coopman, P.; Verhasselt, B.; Bracke, M. et al

in Clinical & Experimental Metastasis (1991), 9(5), 469-84

Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by ... [more ▼]

Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha 6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100 micrograms/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin. [less ▲]

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See detailLes télécommunications d'entreprise : influence sur le développement régional et la répar­tition spatiale des activités
Jacob, Damien ULg

in Bulletin de la Société Belge d'Etudes Géographiques = Tijdschrift van de Belgische Vereniging voor Aardrijkskundige Studies (1991)

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See detailAdult respiratory distress syndrome: local or systemic disease
Deby, Ginette ULg; Lamy, Maurice ULg; Deby, C. et al

in Critical Care (1991), 4(1), 57-83

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See detailEnquete serologique sur la prevalence de l'infection par l'herpes virus bovin type 4 dans les troupeaux bovins des plaines d'Accra au Ghana.
Marchot, Philippe; Thiry, Etienne ULg; Jetteur, P. et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (1991), 44(4), 405-6

Sera from cattle of the Accra Plains in Ghana were screened for bovine type-4 herpesvirus (BHV-4) antibodies by an indirect immunofluorescence test. Among the 176 screened serums, 14% were found positive ... [more ▼]

Sera from cattle of the Accra Plains in Ghana were screened for bovine type-4 herpesvirus (BHV-4) antibodies by an indirect immunofluorescence test. Among the 176 screened serums, 14% were found positive at a 1/100 dilution. Further studies are necessary for a better understanding of the relationships between BHV-4 or some other agents and necrotic vaginitis associated with poor fertility observed in cattle in this area. [less ▲]

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See detailEtude lithostratigraphique, biostratigraphique et sédimentologique du sondage de Lessines (bord méridional du Massif de Brabant, Belgique)
Herbosch, A.; Vanguestaine, M.; Degardin, J. M. et al

in Anales de la société géologique de belgique (1991), 114

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See detailPancreatic failure in relation to trauma and sepsis in intensive care patients
Lamy, Maurice ULg; Deby, Ginette ULg; Damas, Pierre ULg et al

in Applied Cardiopulmonary Pathophysiology [=ACP] (1991), 4

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See detailTAXONOMIC STUDY OF MARINE OSCILLATORIACEAN STRAINS (CYANOPHYCEAE, CYANOBACTERIA) WITH NARROW TRICHOMES .1. MORPHOLOGICAL VARIABILITY AND AUTECOLOGICAL FEATURES
Wilmotte, Annick ULg

in Archiv für Hydrobiologie (1991), 92

Twenty marine and one freshwater strain, belonging to the genera Oscillatoria, Phormidium, Plectonema and Spirulina (sensu GEITLER), exhibiting a cell width less than 3-mu-m were cultivated in different ... [more ▼]

Twenty marine and one freshwater strain, belonging to the genera Oscillatoria, Phormidium, Plectonema and Spirulina (sensu GEITLER), exhibiting a cell width less than 3-mu-m were cultivated in different light intensities, temperatures and salinities. The study of the morphological variability allows one to determine the stable and variable morphological characters. The tolerances to the culture conditions, the possibility of survival in unfavourable conditions and the capacity of chromatic adaptation were investigated. The autecological characters are discussed in relation to the original habitat as well as to the taxonomic grouping of the strains based on the morphology. [less ▲]

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See detailLa formation des utilisateurs des bibliothèques. Une analyse réalisée par un groupe de l'ABD
Pochet, Bernard ULg

in Cahiers de la Documentation = Bladen voor Documentatie (1991), 45(2), 52-55

Cet article décrit la première enquête sur les formations organisées en Belgique, organisée par le groupe formation documentaire de l'ABD

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See detailTumeurs developpees apres ureterosigmoidostomie (U.S.S.). Revue de la litterature a propos de deux cas cliniques.
Nicolas, H.; Bonnet, Pierre ULg; Vandeberg, Colette ULg et al

in Acta Urologica Belgica (1991), 59(4), 73-82

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See detailLes Pachytheca de La Gileppe et de Nonceveux (Dévonien inférieur de Belgique)
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1991), 113

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See detailSpectroscopic investigation of structure in octarellin (a de novo protein designed to adopt the alpha/beta-barrel packing)
Beauregard, Marc; Goraj, Karine; Goffin, Vincent et al

in Protein Engineering (1991), 4(7), 745-9

We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990) Protein ... [more ▼]

We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990) Protein Engng, 3, 259-266]. Infrared and Raman spectra analyses of octarellin's secondary structure reveal the expected percentage of alpha-helices (30%) and a higher beta-sheet content (40%) than predicted from the design. When the Raman spectra obtained with octarellin and native triosephosphate isomerase (a natural alpha/beta-barrel) are compared, similar percentages of secondary structures are found. Thermal denaturation of octarellin monitored by CD confirms that its secondary structures are quite stable, whereas its native-like tertiary fold is not. Tyrosine residues, predicted to be partially hidden from solvent, are actually exposed as revealed by Raman and UV absorption spectra. We conclude that the attempted alpha/beta-barrel conformation in octarellin may be loosely packed. The criteria used to design octarellin are discussed and improvements suggested. [less ▲]

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See detailL'examen cytogénétique en onco-hématologie : son importance dans la stratégie thérapeutique
Collignon, J.; Laurent, C.; Rombout, S. et al

in Médecine et Hygiène (1991), 49

The findings that chromosomal aberrations are specifically associated with a considerable number of tumors and particularly leukemia, have lead the cytogenetic studies to be considered particularly ... [more ▼]

The findings that chromosomal aberrations are specifically associated with a considerable number of tumors and particularly leukemia, have lead the cytogenetic studies to be considered particularly contributive in haematological practices. Since the discovery of th Philadelphia chromosome linked with the chronic myeloid leukemia, technological improvements have allowed detection of others chromosomes specifically associated with some types or subtypes of leukemia, lymphomas and solid tumors. Today, cytogenetics is considered as a useful tool not only for diagnostic but also for prognosis and therapeutic. During the follow-up, it may assess not only complete remission, success of bone marrow transplantation but also relapse. Bone marrow cytogenetic studies are being now essential at diagnosis and during follow-up of patients with hematological proliferative tumors. Well done, cytogenetics studies may be of importance for the therapeutic decision. [less ▲]

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See detailReproductive Development in Lolium temulentum L.: Spike Morphogenesis and Grain Set Limitations
Périlleux, Claire ULg; Bernier, Georges ULg; Kinet, Jean Marie

in Journal of Experimental Botany (1991), 42(4), 501

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was ... [more ▼]

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was to assess whether Lolium temulentum strain Ceres, a qualitative long-day grass, could serve as a model of reproductive development for cereals. The morphological patterns from floral transition to seed set were studied. [less ▲]

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See detailScale economies and performances in the French Insurance Industry
Fecher-Bourgeois, Fabienne ULg; Kessler, D.; Pestieau, Pierre ULg et al

in Geneva Papers on Risk & Insurance - Issues & Practice (1991), (60),

The purpose of this paper is to provide for both life and non life insurance a measurement of economies of scale and an assessment of productive performance of French companies. We use a translog cost ... [more ▼]

The purpose of this paper is to provide for both life and non life insurance a measurement of economies of scale and an assessment of productive performance of French companies. We use a translog cost function to check the presence of scale economies and a parametric approach to construct our yardstick of productive efficiency. Our panel data covers 84 life and 243 non life companies for the period 1984–89. The main findings indicate both the existence of increasing returns to scale and a wide dispersion in the rates of inefficiency across companies. Those two results are shown to be closely linked to a number of characteristics of the companies studied, particularly their institutional form. [less ▲]

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See detailInfluence of Electronic Excitation in the Charge Reversal of halogenated Cations.
Leyh, Bernard ULg; Wankenne, H.

in International Journal of Mass Spectrometry and Ion Processes (1991), 107

Charge reversal of high kinetic energy (8keV) cations during collisions with atomic target gases (G=Xe, Kr, Ne) has been studied for CCln+ (n=1 or 2) ions resulting from dissociative electroionization of ... [more ▼]

Charge reversal of high kinetic energy (8keV) cations during collisions with atomic target gases (G=Xe, Kr, Ne) has been studied for CCln+ (n=1 or 2) ions resulting from dissociative electroionization of CCl4. The influence of electronic excitation of the incident cation CCln+ and of the nature of the target gas G on the anion yield have been examined. In our experiments, the two-collision process CCln+->CCln+G+->(CCln-)+2G+ is predominant. By comparing the anion and cation yields as a function of the kinetic energy of the ionizing electrons, we found evidence, with some target gases, of the existence of long-lived excited states of CCl+ and CCl2+, which lead to an increase in the anion yield in comparison with charge reversal of the correponding ground state ions. For CCl+, we deduce an excitation energy of 4+/-1 eV with a lifetime longer than 6.2 µs. These results are compatible with the a3Pi long-lived state of CCl+ previously detected by optical spectroscopy and by charge-stripping experiments. Our experimental data on charge reversal and metastable dissociation of CCl2+ reveal the existence of a long-lived state (lifetime>8.2 µs) of CCl2+ lying at 4.3+/-1 eV above the ground state.Semi-empirical MNDO calculations show that the energy of the first quartet state, a4A2, is compatible with the observed excitation energy. The spin-orbit coupling-induced transition from the a4A2 state to the B2A2 state is suggested to play an important role in the metestable dissociation CCl2+*->(CCl+)+Cl. The origin of the increase in the anion yield when long-lived excited states of CCl+ and CCl2+ are charge-reversed is discussed. Step a is suggested to be responsible for this yield enhancement which is observed when an appropriate target gas makes this step quasi-resonant. [less ▲]

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See detailMORPHOLOGICAL AND GENETIC CRITERIA IN THE TAXONOMY OF CYANOPHYTA CYANOBACTERIA
Wilmotte, Annick ULg; Golubic, Stjepko

in Archiv für Hydrobiologie (1991), 92

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte ... [more ▼]

Molecular techniques have introduced new approaches to the phylogeny and taxonomy of Cyanophyta/Cyanobacteria. One method, the sequencing of the 16S ribosomal RNA, has been applied to 29 cyanophyte strains and produced interesting results (TOMIOKA & SUGIURA 1983, GIOVANNONI et al. 1988). Integration of this new, taxonomically useful information with the results of the classical morphological analyses traditionally used in the taxonomy of cyanophytes is the subject of this paper. Advantages and limitations of different approaches are discussed, and conflicts as well as consensus between morphological properties of the strains under study, and their position in the phylogenetic tree of GIOVANNONI et al. (1988) are reviewed. [less ▲]

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See detailAN APPROACH TO THE ASYMMETRIC-SYNTHESIS OF L-6-[F-18]FLUORODOPA VIA NCA NUCLEOPHILIC FLUORINATION
Lemaire, Christian ULg; Guillaume, Marcel; Cantineau, Robert et al

in Applied Radiation & Isotopes (1991), 42(7), 629-635

The NCA asymmetric synthesis of L-6-[F-18]fluorodopa starting from (1R,2R,5R)-[(+)-2-hydroxypinanyl-3-idene]glycine t-butyl ester as chiral agent has been developed. After F-18-fluorination of the two ... [more ▼]

The NCA asymmetric synthesis of L-6-[F-18]fluorodopa starting from (1R,2R,5R)-[(+)-2-hydroxypinanyl-3-idene]glycine t-butyl ester as chiral agent has been developed. After F-18-fluorination of the two commercially available aldehydes either 6-nitroveratraldehyde or 6-nitropiperonal, the required alkylating [F-18]fluorobenzyl bromide derivative can be easily prepared by treatment with NaBH4 followed by SOBr2. Alkylation of the Schiff base was carried out with the lithium salt of 2,2,6,6-tetramethylpiperidine as base in anhydrous THF at -78-degrees-C. Following hydrolysis of the protecting groups with hydroxylamine and Hl, the L-amino acid was obtained in 75% L form (ee 50%) with a 10% decay corrected (120 min) radiochemical yield. [less ▲]

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See detailPAF receptor structure: a hypothesis
Godfroid, Jean-Jacques; Dive, Georges ULg; Lamotte-Brasseur, Josette et al

in Lipids (1991), 26(12), 1162-1166

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See detailSkeleton resorption in echinoderms : regression of pedicellarial stalks in Sphaerechinus granularis (Echinoidea)
Bureau, Fabrice ULg; Dubois, Philippe; Ghyoot, M. et al

in Zoomorphology (1991), 110

When a globiferous pedicellaria of Sphaerechinus granularis injects its venom, the head autotomizes whereas the stalk remains on the test and enters a regression process with concomitant resorption of its ... [more ▼]

When a globiferous pedicellaria of Sphaerechinus granularis injects its venom, the head autotomizes whereas the stalk remains on the test and enters a regression process with concomitant resorption of its supporting ossicle (i.e. the rod). Scanning electron microscope investigations of the morphological changes undergone by the stereom of resorbing rods show that: (1) resorption proceeds both axially and laterally, and leads to a reduction of approximately 80% of the original length of the rod, (2) secondary growth of new stereom processes occurs concomitantly with resorption but never ensures even a partial regeneration of the rod, and (3) resorption and secondary growth stop before the rod is totally destroyed leaving a static stump that remains in place up to 190 days. Particular resorption figures result from either the axial or the lateral resorption of the rod shaft. These consist chiefly of terraced conical cupules, dense cylinders and concentric lamellae whose walls or edges are typically made of closely piled and/or aligned subprismatic crystallites. Whatever their location along the rod, these crystallites always organize strictly parallel to the rod axis. Whether the crystallites are mosaic blocks composing larger monocrystalline units or discrete monocrystals themselves is for the moment unclear. A growth model, which accounts for the observed resorption figures, is proposed for the shaft of pedicellarial rods. According to this model, the early growth of the shaft would produce elongated, interconnected trabeculae (initial trabeculae) made of densely piled and perfectly aligned crystallites. Thickening and coalescence of adjoining trabeculae would progressively occur by adjunction around the initial trabeculae of successive and concentric layers of similarly arranged crystallites. Coalescent trabeculae would then be cemented together in a perforate stereom layer by the final deposition of larger crystallite layers surrounding the whole shaft periphery. Growth of secondary stereom processes occurs both in the resorbing rod (here the newly formed processes are resorbed soon after they have been produced) and in rods where resorption has stopped. These are always irregular processes that localize near or on the actual sites of resorption. It is suggested these processes result from an uncontrolled activation of the skeleton-forming cells in areas where the concentration of calcium ions increases as a consequence of calcite resorption [less ▲]

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See detailEntre Meuse, Rhin et Moselle. Aux origines du culte de saint Mengold de Huy
George, Philippe ULg

in Publications de la Section Historique de l'Institut Royal Grand-Ducal de Luxembourg (1991)

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See detailThe evolutionarily conserved Kruppel-associated box domain defines a subfamily of eukaryotic multifingered proteins
Bellefroid, Eric J; Poncelet, Dominique A; Lecocq, Pierre J et al

in Proceedings of the National Academy of Sciences of the United States of America (1991), 88(9), 3608-12

We have previously shown that the human genome includes hundreds of genes coding for putative factors related to the Kruppel zinc-finger protein, which regulates Drosophila segmentation. We report herein ... [more ▼]

We have previously shown that the human genome includes hundreds of genes coding for putative factors related to the Kruppel zinc-finger protein, which regulates Drosophila segmentation. We report herein that about one-third of these genes code for proteins that share a very conserved region of about 75 amino acids in their N-terminal nonfinger portion. Homologous regions are found in a number of previously described finger proteins, including mouse Zfp-1 and Xenopus Xfin. We named this region the Kruppel-associated box (KRAB). This domain has the potential to form two amphipathic alpha-helices. Southern blot analysis of "zoo" blots suggests that the Kruppel-associated box is highly conserved during evolution. Northern blot analysis shows that these genes are expressed in most adult tissues and are down-regulated during in vitro terminal differentiation of human myeloid cells. [less ▲]

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See detailObservations ultraviolettes des planètes géantes et de leurs satellites à l'aide du Télescope Spatial Hubble
Gérard, Jean-Claude ULg; Dols, Vincent

in Nouvelles de la Science et des Technologies (1991)

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See detailPhagocytose immune et non immune
Malaise, Michel ULg

in Revue Médicale de Liège (1991), 46(9), 486-503

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See detailIntérêt de la différence aterio-veineuse en O2 dans l'approche thérapeutique de l'hypertension intracrânienne post-traumatique
Franssen, Christine ULg; Hans, Pol ULg; Deprez, Josette ULg et al

in Agressologie : Revue Internationale de Physio-Biologie et de Pharmacologie Appliquées aux Effets de l'Agression (1991), 32(6-7), 366-368

Elevated intracranial pressure (ICP) is undoubtedly a determinant factor of outcome in severe head injured patient. Until now, medical treatment of elevated ICP in diffuse brain lesions consisted of ... [more ▼]

Elevated intracranial pressure (ICP) is undoubtedly a determinant factor of outcome in severe head injured patient. Until now, medical treatment of elevated ICP in diffuse brain lesions consisted of hyperventilation, CSF drainage, mannitol and barbiturates without accurate selection criteria. In fact, in comatose patients, the cerebral blood flow (CBF) can vary independently of the cerebral metabolic rate of oxygen (CMRO2) which is typically reduced. The venous oxygen saturation (SjO2) measured in the jugular bulb and the arterial-jugular oxygen difference (ajDO2) can be used for more appropriately selecting therapeutic measures. According to the following equation: CMRO2 = CBF x ajDO2, the measurement of the changes in ajDO2 might serve as an indicator of CBF adequacy, out of the presence of cerebral ischemia. A decreased ajDO2 suggests that CBF is excessive for cerebral metabolic requirements and an elevated ajDO2 indicates a decreased CBF. Consequently, treatment of elevated ICP in the presence of a low ajDO2 should be more oriented towards moderate hyperventilation and administration of metabolic depressive agents with maintenance of normal arterial blood pressure. Conversely, in case of high ajDO2, ICP control should aim to increase CBF by maintaining normocapnia, improving hemodynamic status or reducing extravascular volume accordingly. [less ▲]

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See detailPour un roman sans manifeste
Demoulin, Laurent ULg

in Ecritures (1991), 1

Description des nouvelles tendances du roman écrit en français.

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See detailAutomatic Determination of Diltiazem and Desacetyldiltiazem in Human Plasma Using Liquid-Solid Extraction on Disposable Cartridges Coupled to Hplc--Part I: Optimization of the Hplc System and Method Validation
Hubert, Philippe ULg; Chiap, Patrice ULg; Crommen, Jacques ULg

in Journal of Pharmaceutical & Biomedical Analysis (1991), 9(10-12), 877-882

A sensitive and automated method for the analysis of diltiazem and desacetyldiltiazem in plasma has been developed using liquid-solid extraction (LSE) on disposable extraction cartridges (DECs) in ... [more ▼]

A sensitive and automated method for the analysis of diltiazem and desacetyldiltiazem in plasma has been developed using liquid-solid extraction (LSE) on disposable extraction cartridges (DECs) in combination with HPLC. After isolation from plasma, the analytes are separated on a highly deactivated octyl silica column with a mobile phase of methanol-0.05 M phosphate buffer (pH 7.4) (62:38, v/v). The analytes are monitored photometrically at 238 nm. The complete preparation of the plasma sample as well as the injection of the final extract on to the analytical column are performed automatically by means of a sample processor equipped with a robotic arm to which is attached a needle dispensing the different liquids. The internal standard solution is first added to the plasma sample. The DEC is then conditioned successively with methanol and phosphate buffer (pH 7.4). A 1.0-ml volume of sample containing the internal standard solution is applied on an extraction cartridge filled with cyanopropyl silica (50 mg). After the DEC has been washed with the same buffer, the analytes are eluted with 0.16 ml of methanol. A 0.14-ml volume of buffer is then passed through the DEC and 0.25 ml of the final extract is injected onto the HPLC column. The absolute recoveries of the drugs are about 90% and the limit of detection for diltiazem is 0.8 ng ml-1. Relative standard deviations of 2.6% (within-day) and 3.7% (between-day) have been obtained for this compound at a plasma concentration of 50 ng ml-1. [less ▲]

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See detailCloning and characterization of a cDNA encoding the bovine insulin-like growth factor binding protein 1 (bIGFBP-1).
Sneyers, M.; Kettmann, Richard ULg; Massart, Serge et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1991), 1(6),

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See detailConserved KRAB protein domain identified upstream from the zinc finger region of Kox 8
Thiesen, H. J.; Bellefroid, E.; Revelant, O. et al

in Nucleic Acids Research (1991), 19(14), 3996

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See detailVelogenetics or the synergistic use of Marker Assisted Selection and Germ-Line Manipulation
Georges, Michel ULg; Massey, J. M.

in Theriogenology (1991), 35(1), 151-156

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See detailLe triticale en alimentation animale. Les volailles.
Leterme, Pascal; Thewis, André ULg

in Revue de l’Agriculture = Landbouwtijdschrift (1991), 44(5), 945-951

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See detailApplications des progestagènes au traitement de l'anoestrus fonctionnel dans l'espèce bovine.
Hanzen, Christian ULg; Laurent, Yves

in Annales de Médecine Vétérinaire (1991), 135

Detailed reference viewed: 65 (3 ULg)
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See detailUptake kinetic of medium constituants during batch growth of patatoe cell cultures.
Gerard, J.; Antoine, P.; Lepoivre, Philippe ULg et al

in Biotechnology Letters (1991), 13(5), 381-384

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See detailAmino acid composition and digestibility of four protein sources for dogs.
Neirinck, K.; Istasse, Louis ULg; Gabriel, Annick ULg et al

in Journal of Nutrition (1991), 121(11 Suppl), 64-5

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See detailSiderophores production and Enhancement by optimization of culture conditions and transposon-mutagenesis of a fluorescent Pseudomonas.
Delfosse, Ph; Jacques, Ph; Moussaif, M. et al

in Archives Internationales de Physiologie et de Biochimie (1991), 99(B55),

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See detailPit-1 binding sequences permit calcium regulation of human prolactin gene expression
Hoggard, Nigel; Davis, Julian R E; Berwaer, Monique et al

in Molecular Endocrinology (1991), 5(11), 1748-54

This study examines the regulation of the human PRL (hPRL) gene promoter by intracellular calcium. Deletants of the 5'-flanking region of the hPRL gene and constructs consisting of the thymidine kinase ... [more ▼]

This study examines the regulation of the human PRL (hPRL) gene promoter by intracellular calcium. Deletants of the 5'-flanking region of the hPRL gene and constructs consisting of the thymidine kinase promoter linked to the first or second proximal Pit-1 binding site were fused to the bacterial chloramphenicol acetyl transferase (CAT) reporter gene. With the complete 5-kilobase pair (kbp) hPRL promoter sequence the calcium channel agonist Bay K8644 induced a significant 2-fold increase in CAT reporter gene expression and the antagonist verapamil a 4.5-fold reduction, using GH3 cells cultured in physiological levels of calcium. The transcriptional response to calcium influx was similar with a series of 5'-deleted hPRL-CAT constructs including those that comprised the proximal (up to 740 bp) or distal (-1300- to -1700-bp) sequences alone. When treating cells cultured in low calcium conditions the induction with the hPRL promoter increased to 5-fold on the addition of exogenous calcium and Bay K8644. The pituitary-specific expression of the hPRL gene is conferred by the interaction of the pituitary-specific factor Pit-1 with several binding sites located in the 5'-flanking DNA, of which three are located in the proximal region. This suggested that Pit-1 binding sites may be involved in the calcium response.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailL'intérêt porté par les poètes et les traducteurs russes à l'Ode III, 30 d'Horace (Exegi monumentum)
Mouton, Charles ULg

in Latomus : Revue d'Etudes Latines (1991), L(3), 654-670

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See detailSociabilités, aides intrafamiliales et pauvreté
Born, Michel ULg

in PSELL (1991)

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See detailWirksamkeit eines oralen Beruhigungsmittels auf das Verhalten und die zootechnischen Leistungen von Mastbullen der Wei-Blauen Belgischen Rasse
Genicot, Bruno; Mouligneau, Frédéric; Lekeux, Pierre ULg

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (1991), 38

This trial was conducted with 22 Belgian White and Blue double-muscled cattle (body weight: 241.5 +/- 6.2 kg; age: 7 months). Two similar groups were constituted: a group of bulls which were treated with ... [more ▼]

This trial was conducted with 22 Belgian White and Blue double-muscled cattle (body weight: 241.5 +/- 6.2 kg; age: 7 months). Two similar groups were constituted: a group of bulls which were treated with potassium bromide (BT; n = 11) and another one which remained untreated UT; n = 11). The treatment (736 mg potassium bromide/kg feed dry matter) was stopped 60 days before the slaughtering day. The treatment with potassium bromide induced a significantreduction in the rear engagements during the whole trial period (221 days), in direct attacks during the period A (from day 0 to day 53) and in side-on attacks during the period B from day 54 to day 167). The daily weight gains, calculated for the whole trial period, were not significantly different between the two groups. Feed utilization was lower in treated bulls. During the period C (from day 168 to day 221), the sedative potency of potassium bromide seemed to be weaker than during the periods A and B. This might be due to the habituation to potassium bromide. On the basis of these results, we can conclude that the frequency of rear engagements and, during a restricted period of fattening, the frequencies of side and directattacks are significantly reduced when potassium bromide (736 mg/kg feed dry matter) is used. The time spent for hay intake, the frequency of water intake (even if the time consumed at the drinking trough was the same) was reduced and the time during which the bulls were standing was prolonged by bromide [less ▲]

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See detailLe triticale en alimentation animale. Les ruminants.
Thewis, André ULg; Leterme, Pascal

in Revue de l’Agriculture = Landbouwtijdschrift (1991), 44(4), 707-716

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See detailVolonté, ordre public et fraude dans la reconnaissance des divorces et répudiations intervenus à l'étranger
Carlier, Jean-Yves ULg

in Revue Trimestrielle de Droit Familial [=RTDF] (1991)

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See detailDigestibilite ileale des acides amines de regimes monocereale-tourteau de soja chez le porc.
Leterme, Pascal; Thewis, André ULg; Thielemans, Marie France et al

in Revue de l’Agriculture = Landbouwtijdschrift (1991), 44(3), 423-432

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See detailLocation of rRNA gene transcription sites within Ehrlich tumor cell nucleoli.
Thiry, Marc ULg

in Bulletin de l'Association des Anatomistes (1991), 75(229), 33-7

The nucleolus is the morphological visualization of ribosome biogenesis. The spatial distribution of nucleolar DNA has been investigated in Ehrlich tumor cells by various immunogold labelling techniques ... [more ▼]

The nucleolus is the morphological visualization of ribosome biogenesis. The spatial distribution of nucleolar DNA has been investigated in Ehrlich tumor cells by various immunogold labelling techniques. The DNase 1-sensitive regions within the nucleolus have been identified by in situ nick-translation at the ultrastructural level. The precise nucleolar location of rDNA and rRNA has been further specified by in situ hybridization and electron microscopy. Our results indicate that the fibrillar centers are the sole nucleolar structures where DNase 1-hypersensitive sequences, rDNA and rRNA are located together. These findings strongly suggest that rRNA gene transcription takes place within the confines of the fibrillar center, probably close to the boundary regions to the surrounding dense fibrillar component. [less ▲]

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See detailThe Bovine Leukemia-Virus Tax Gene Contains An Enhancer Sequence
Willems, Luc ULg; Romond, Pc.; Ghysdael, J. et al

in Virology (1991), 182(1),

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See detailIntérêt de l'électromyostimulation de basse fréquence dans la rééducation du quadriceps
Constant, T.; Vanderthommen, Marc ULg

in STAPS : Revue Internationale des Sciences du Sport et de l'Education Physique (1991), 24

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See detailUn cas de syndactylie chez le chien
RENOY, Philippe ULg; Balligand, Marc ULg

in Annales de Médecine Vétérinaire (1991)

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See detailDescription des restes contenus dans un échantillon concrétionné découvert au Titelberg.
Gerrienne, Philippe ULg; Fairon-Demaret, Muriel ULg; Cordy, Jean-Marie ULg

in Bulletin de la Société Préhistorique du Luxembourg (1991), 13

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See detailRefinement of the media composition for the in vitro culture of early heart-shaped embryos in Phaseolus vulgaris.
Schmit, V.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in Annual report of the Bean Improvement Cooperative (1991), 34

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See detailACCUMULATION OF ACYL-ENZYME IN DD-PEPTIDASE-CATALYZED REACTIONS WITH ANALOGS OF PEPTIDE-SUBSTRATES
JAMIN, M.; Adam, Maggy; Damblon, Christian ULg et al

in Biochemical Journal (1991), 280(Part 2), 499-506

Thioester substrates can be used to study the hydrolysis and transfer reactions catalysed by beta-lactamases and DD-peptidases. With the latter enzymes, accumulation of the acyl-enzyme can be detected ... [more ▼]

Thioester substrates can be used to study the hydrolysis and transfer reactions catalysed by beta-lactamases and DD-peptidases. With the latter enzymes, accumulation of the acyl-enzyme can be detected directly. The efficiency of various amines as acceptor substrates was in excellent agreement with previous results obtained with peptide substrates of the DD-peptidases. The results indicated the presence of a specific binding site for the acceptor substrates, Although most of the results agreed well with a simple partition model, more elaborate hypotheses will be needed to account for all the data presented. [less ▲]

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See detailL'agriculture dans la Province du Luxembourg
Burny, Philippe ULg

in Annales de Gembloux (1991), 97(1), 43-67

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See detailSupply dependency of oxygen uptake during endotoxin insult and volume resuscitation
D'Orio, Vincenzo ULg; FATEMI, M; MENDES, M et al

in Applied Cardiopulmonary Pathophysiology [=ACP] (1991), 4

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See detailDermal Dendrocytes and Photochemotherapy
Pierard, Gérald ULg; Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1991), 418(4), 311-314

We studied the fate of dermal dendrocytes in patients treated with psoralens and ultraviolet light by combining immunohistochemistry and computerized image analysis. Factor-XIIIa-positive dermal ... [more ▼]

We studied the fate of dermal dendrocytes in patients treated with psoralens and ultraviolet light by combining immunohistochemistry and computerized image analysis. Factor-XIIIa-positive dermal dendrocytes were found to be altered in these patients. When compared with controls, dermal dendrocytes were often increased in number and had an uneven size and tissue distribution. Their cytoplasm was occasionally fragmented. These changes were more pronounced when early photosclerosis was present. The alterations described probably reflect vascular changes, and may be responsible for immunomodulating actions and disorder of the connective tissue structure induced by ultraviolet light. [less ▲]

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See detailExperimental study of the non-linear behaviour of beam-to-column composite joints
Altmann, R.; Maquoi, René ULg; Jaspart, Jean-Pierre ULg

in Journal of Constructional Steel Research (1991), 18

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See detailStrategy of analysis and interpretation of thermal working conditions.
Malchaire, Jacques; Mairiaux, Philippe ULg

in Annals of Occupational Hygiene (1991), 35(3), 261-72

This paper proposes a strategy for the management of heat problems at the workplace. This includes three steps of analysis: the first aims at recognizing the problem and classifying it under one of the ... [more ▼]

This paper proposes a strategy for the management of heat problems at the workplace. This includes three steps of analysis: the first aims at recognizing the problem and classifying it under one of the categories--comfort, discomfort but no health risk, or health risk either in the long term or in the short term. The second is designed to evaluate the magnitude of the problem and to optimize the choice of solutions. The interpretation of the data is based on analytical indices: the PMV-PPD indices for heat discomfort conditions and the Required Sweat Rate index for heat stress conditions. The third step involves an in-depth analysis of the situation: this will be undertaken only if a major problem of heat stress has been detected at the previous stage and providing technical solutions cannot be immediately implemented. The advantages and possible limitations of this step-by-step approach are outlined and its cost effectiveness is discussed in the perspective of a true management of health and safety at work. [less ▲]

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See detailLa dendrochronologie au service de la datation des monuments
Hoffsummer, Patrick ULg

in Bulletin de la Fondation Vanhove-Vonneche (1991), 3

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See detailEstudio morfométrico del Aspergillus en lesiones humanas
Arrese Estrada, Jorge ULg; Rurangirwa, A.; Nikkels, Arjen ULg et al

in Revista Iberoamericana de Micología (1991), 8

The morphology of Aspergillus hyphae in diffuse pulmonary aspergillosis were studied by computarized image analysis. We considered as parameters the angles of the mycelia. These data were constrasted ... [more ▼]

The morphology of Aspergillus hyphae in diffuse pulmonary aspergillosis were studied by computarized image analysis. We considered as parameters the angles of the mycelia. These data were constrasted, with similar morphometric evaluations of Pseudalleschria boydii in invasive pulmonary pseudallescheriasis. A significant difference was found between the degrees of branching angles in these two fungi. Conversely, there was no significant difference between the total surface areas covered by the two fungi. The above data suggest a potential interest of morphometry in mycology. [less ▲]

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See detailNEW ROTATIONAL ANALYSIS OF THE ALPHA-1-PI-CHI-1-SIGMA+ TRANSITION OF ALBR
BREDOHL, Harald; DUBOIS, Iwan; Mahieu, Emmanuel ULg et al

in Journal of Molecular Spectroscopy (1991), 145(1), 12-17

Twenty-eight bands of the A1Π-X1Σ+ transition have been fully analyzed. The vibrational constants obtained for the A1Π state do not agree with earlier data. On the other hand the observations suggest that ... [more ▼]

Twenty-eight bands of the A1Π-X1Σ+ transition have been fully analyzed. The vibrational constants obtained for the A1Π state do not agree with earlier data. On the other hand the observations suggest that the conclusion about the predissociation ought to be revised. [less ▲]

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See detailTravail sur écran de visualisation. Un risque pour la grossesse ?
Mairiaux, Philippe ULg

in Louvain Medical (1991), 110(3), 185-189

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See detailOn the characterization of some terpenes from Renealmia alpinia Rott. (Maas) oleoresin.
Lognay, Georges ULg; Marlier, M.; Severin, M. et al

in Flavour and Fragrance Journal (1991), 6(1),

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See detailThe cytokines: a possible role in sepsis
Damas, Pierre ULg

in Current Opinion in Anaesthesiology (1991), 4(2), 241-246

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See detailPassage légal et passage conventionnel. Variations sur le possessoire et le pétitoire
Lecocq, Pascale ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (1991)

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See detailLe serment de Paneb et de son fils (P Salt 124, V° 1,5-8)
Winand, Jean ULg

in Bulletin de la Société Égyptologique de Genève (1991), 15

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See detailNeuroendocrine-Immunology: from systemic interactions to the immune tolerance of self neuroendocrine functions
Geenen, Vincent ULg; Robert, Françoise; Legros, Jean-Jacques ULg et al

in Acta Clinica Belgica (1991), 46

In recent years, it appeared more and more that the three major integrating and adaptive systems of intercellular communication, nervous, endocrine, and immune systems, are closely interconnected. Through ... [more ▼]

In recent years, it appeared more and more that the three major integrating and adaptive systems of intercellular communication, nervous, endocrine, and immune systems, are closely interconnected. Through these interactions, psychological and neurological influences can modulate the immune response (neuroimmunomodulation), while immune cells may communicate to the neuroendocrine system by a regulatory feedback loop. On the basis of our own observations, it has been shown that the neuroendocrine-immune dialogue occurs in the thymus during the early steps of T-cell differentiation, and could be involved both in T-cell positive as well as negative selections. [less ▲]

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See detailCytokines et choc septique
Lamy, Maurice ULg; Damas, Pierre ULg

in Bulletin de l'Académie Royale de Médecine de Belgique (1991), 146(6-7), 270

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See detailSolubilization of Thiamine Triphosphatase from the Electric Organ of Electrophorus Electricus
Bettendorff, Lucien ULg; Longree, Isabelle; Wins, Pierre et al

in Biochimica et Biophysica Acta - General Subjects (1991), 1073(1), 69-76

The membrane-associated, anion-regulated thiamine triphosphatase from Electrophorus electricus electric organ can be solubilized by various neutral detergents. Polyoxyethylene ethers are the most ... [more ▼]

The membrane-associated, anion-regulated thiamine triphosphatase from Electrophorus electricus electric organ can be solubilized by various neutral detergents. Polyoxyethylene ethers are the most effective. Anionic detergents readily inactivate the enzyme. A 6.4-fold increase in specific activity is obtained by successive treatment of crude membranes with octanoyl-N-methylglucamide, which solubilized other proteins, and Lubrol-PX with releases 60% of the thiamine triphosphatase (TTPase) activity. Solubilization by Lubrol-PX rapidly modifies kinetic parameters. The Km, Vmax and pH optimum are decreased. However, the solubilized TTPase may be kept at 0 degrees C for many hours without further change in specific activity. At 35 degrees C, the half-life is still 83 min at pH 5.0, but denaturation becomes rapid at pH greater than or equal to 7. Solubilization modifies anion effects on TTPase activity. The activating effect of nitrate is nearly lost, while inhibition by sulfate is no longer time-dependent. [less ▲]

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See detailItaly : two Perspectives. Racism in Paradise ?
Martiniello, Marco ULg; Kazim, P.

in Race & Class (1991), 32(3), 79-89

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See detailPhotosystem II assembly in 2-day-old bean leaves during the first 16 hrs. of greening
Schoefs, Benoît; Franck, Fabrice ULg

in Comptes Rendus de l'Académie des Sciences. Série III, Sciences de la Vie (1991), 313(10), 441-445

We have studied the assembly of the photosynthetic apparatus during the greening of 2-day-old bean leaves in continuous white light by using fluorimetric methods. We have recorded 77 and 298 K ... [more ▼]

We have studied the assembly of the photosynthetic apparatus during the greening of 2-day-old bean leaves in continuous white light by using fluorimetric methods. We have recorded 77 and 298 K fluorescence kinetics at 690 nm in order to detect electron flow through the photosystem II reaction centre (RC(II)) at increasing greening periods. In those experiments, the 77 K fluorescence spectra were also considered. Charge separation is detected 1 hr. after the onset of the illumination. Room temperature fluorescence variation showing the << O-I-P >> phases are detected after 4 hrs. of illumination. Typical fluorescence bands at 688, 697 and 735 nm appear after 14 hrs. of illumination. [less ▲]

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See detailA method to compute reactive power margins with respect to voltage collapse
Van Cutsem, Thierry ULg

in IEEE Transactions on Power Systems (1991), 6

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See detailQuantum effects in competitive decay
Remacle, Françoise ULg; Desouter, Michèle ULg; Lorquet, Jean-Claude

in Chemical Physics (1991), 153

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See detailBiostatistical studies on western European Dactylorhiza (Orchidaceae)-the D. maculata group
Dufrêne, Marc ULg; Gathoye, J.-L.; Tyteca, D.

in Plant Systematics & Evolution (1991), 175(1-2), 55-72

Multivariate analysis tools are exploited on a data set composed of quantitative characteristics collected on 35 populations of plants of the Dactylorhiza maculata (L.)Soó group from Western-Europe. These ... [more ▼]

Multivariate analysis tools are exploited on a data set composed of quantitative characteristics collected on 35 populations of plants of the Dactylorhiza maculata (L.)Soó group from Western-Europe. These samples lead to four well-defined clusters; this, together with qualitative, cytological and ecological arguments, allows for the recognition of four specific entities:D. maculata s.str., D. fuchsii (Druce)Soó, D. saccifera (Brongn.)Soó and D. caramulensis (Vermeulen)Tyteca. It is concluded that the floral characters play an essential role in the taxonomical distinction. It also appears that the set of characters measured, as well as the methods exploited, are especially well-suited and valuable tools for the morphological study of the genus Dactylorhiza. © 1991 Springer-Verlag. [less ▲]

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See detailMyeloperoxidase and elastase as markers of leukocyte activation during cardiopulmonary bypass in humans
Faymonville, Marie ULg; Pincemail, Joël ULg; Duchateau, J. et al

in Journal of Thoracic and Cardiovascular Surgery (The) (1991), 102

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See detailWord interruption in self-repairing
Brédart, Serge ULg

in Journal of Psycholinguistic Research (1991), 20(2), 123-137

Levelt (1983) proposed that the reason why speakers often do not interrupt an erroneous word before self-repairing is a lack of trouble detection before the end of that word. However, this explanation ... [more ▼]

Levelt (1983) proposed that the reason why speakers often do not interrupt an erroneous word before self-repairing is a lack of trouble detection before the end of that word. However, this explanation does not apply to merely inappropriate words. According to Levelt, this latter kind of word is completed for pragmatic reasons. In the present paper, a new corpus containing 1225 repairs is analyzed. From Levelt's theoretical framework, it was predicted that for erroneous words the longer the reparandum the higher the amount of word interruptions. Another prediction was that this decrease across word length should be slighter for nonerroneous words than for erroneous ones. Both predictions were confirmed. Results were consistent with Levelt's hypothesis and especially with the idea that erroneous word completion is not a real exception to the main interruption rule in speech self-repairing. [less ▲]

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See detailThe lack of relationship between DST nonsuppression in the dexamethasone suppression test and EEG abnormalities.
Pitchot, William ULg; Hansenne, Michel ULg; Ansseau, Marc ULg

in European Archives of Psychiatry & Clinical Neuroscience (1991), 241(3), 193-4

As a recent study suggested a relationship between cortisol escape following dexamethasone suppression test (DST) and electroencephalogram (EEG) abnormalities, we tried to replicate these findings in 52 ... [more ▼]

As a recent study suggested a relationship between cortisol escape following dexamethasone suppression test (DST) and electroencephalogram (EEG) abnormalities, we tried to replicate these findings in 52 major depressive inpatients. A total of 23 patients exhibited DST nonsuppression (44%) and 18 patients had EEG abnormalities (35%). No relationship existed between DST and EEG results. [less ▲]

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See detailNucleotide sequence of the bovine cyclic-AMP responsive DNA binding protein (CREB2) cDNA.
Willems, Luc ULg; Kettmann, Richard ULg; Chen, G. et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1991), 1(6),

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See detailDistinguishing the sites of pre-rRNA synthesis and accumulation in Ehrlich tumor cell nucleoli.
Thiry, Marc ULg; Goessens, Guy ULg

in Journal of Cell Science (1991), 99 ( Pt 4)

The precise location of transcribing rRNA genes within Ehrlich tumor cell nucleoli has been investigated using two approaches: high-resolution autoradiography of cells pulse-labelled with tritiated ... [more ▼]

The precise location of transcribing rRNA genes within Ehrlich tumor cell nucleoli has been investigated using two approaches: high-resolution autoradiography of cells pulse-labelled with tritiated uridine, varying the exposure time, and in situ-in vitro transcription coupled with an immunogold labelling procedure. When autoradiographic preparations are exposed for a short time, silver grains are found associated almost exclusively with interphasic cell nucleoli. Labelling of extranucleolar areas requires longer exposure. Within the nucleolus, the first sites to be revealed are in the dense fibrillar component. Prolonging exposure increases labelling over the dense fibrillar component, with label becoming more and more apparent over the fibrillar centers. Under these conditions, however, labelling does not extend into the granular component, and no background is observed. Initiation of transcription on ultrathin cell sections occurs preferentially at the borders of condensed chromatin blocks and in their close vicinity. The condensed chromatin areas themselves remain unlabelled. Inside most nucleoli, gold-particle clusters are mainly detected in the fibrillar centers, especially at their periphery, whereas the dense fibrillar component and the granular component remain devoid of label. These results, together with previous observations made on the same cell type, clearly indicate that the fibrillar centers are the sites of rRNA gene transcription in Ehrlich tumor cell nucleoli, while the dense fibrillar component is the site of pre-rRNA accumulation. [less ▲]

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See detailEffects of Strychnopentamine on Cells Cultured in Vitro
Quetin-Leclercq, J.; Gillet, Marie-Claire ULg; Angenot, Luc ULg et al

in Chemico-Biological Interactions (1991), 80(2), 203-16

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human ... [more ▼]

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human fibroblasts cultured in vitro. SP strongly inhibits cell proliferation and induces cell death at a relatively low concentration (less than 1 microgram/ml) after 72 h of treatment in the two lines. Incorporation of [3H]thymidine and [3H]leucine by B16 cells significantly decreases after only 1 h of treatment at 0.5 microgram/ml. SP induces the formation of dense lamellar bodies and vacuolization in the cytoplasm, intense blebbing at the cell surface and various cytological alterations leading to cell death. [less ▲]

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See detailApport du genie genetique en production animale.
Portetelle, Daniel ULg

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(2),

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See detailObservations on seed protein electrophoretic patterns of some species within the genus Phaseolus.
Baudoin, Jean-Pierre ULg; Schmit, V.; Wathelet, Bernard ULg

in Annual Report of the Bean Improvment Cooperative (1991), 34

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See detailCirculating erythropoietin levels after bone marrow transplantation: inappropriate response to anemia in allogeneic transplants.
Beguin, Yves ULg; Clemons, G. K.; Oris, Renée ULg et al

in Blood (1991), 77(4), 868-73

We studied 24 recipients of autologous bone marrow transplantation (ABMT) or allogeneic BMT (BMT) to determine whether impaired erythropoietin (Epo) response to anemia could delay full erythropoietic ... [more ▼]

We studied 24 recipients of autologous bone marrow transplantation (ABMT) or allogeneic BMT (BMT) to determine whether impaired erythropoietin (Epo) response to anemia could delay full erythropoietic recovery. Observed Epo levels were compared with predicted levels based on the relationship between Epo and hematocrit in 125 control subjects. Circulating Epo levels were normal during conditioning and the early posttransplant period. Between days 21 and 180, Epo levels remained normal in ABMT patients but were inappropriately low for the degree of anemia in BMT patients. Median time to full erythropoietic engraftment was longer in BMT than in ABMT recipients. Circulating Epo returned to appropriate levels after day 180, except in patients with active cytomegalovirus infection. We conclude that impaired Epo response to anemia can contribute to delayed erythropoietic recovery after allogenic BMT. Renal toxicity of ciclosporin, interaction between host and donor marrow, and cytomegalovirus infection might play a role. This study could support the use of recombinant human Epo to accelerate erythropoietic engraftment after BMT. [less ▲]

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See detailOstéite sternale et médiastinite après chirurgie de pontage aorto-coronaire
DETROZ, B.; Defraigne, Jean-Olivier ULg; Limet, Raymond ULg

in Annales de Chirurgie (1991), 45(2), 128-35

Between 1980 and 1987, 31 cases of osteitis (n = 9) and/or mediastinitis (n = 22) were observed after 2,801 consecutive aorto-coronary bypasses (1.1%). Three types of treatment were used: 1) sternal ... [more ▼]

Between 1980 and 1987, 31 cases of osteitis (n = 9) and/or mediastinitis (n = 22) were observed after 2,801 consecutive aorto-coronary bypasses (1.1%). Three types of treatment were used: 1) sternal debridement with osteosynthesis and continuous mediastinal irrigation (n = 25); 2) sternal and mediastinal debridement with open drainage without osteosynthesis (n = 2); 3) incision and debridement of sternal abscesses (n = 4). The overall mortality was 26% (8/31), i.e. 11% (1/9) for isolated osteitis and 32% (7/22) for mediastinitis. Four factors were statistically associated with infection: reoperation for hemorrhage (19.4%, p less than 0.001); preoperative diabetes (25%, p less than 0.001), postoperative low cardiac output (55%, p less than 0.001), postoperative respiratory insufficiency (45%, p less than 0.001). [less ▲]

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See detailLa maison de repos, une entreprise de services
Jurion, Bernard ULg

in Association des Directeurs de Maisons de Repos de la Communauté Française (1991), 8

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See detailIncreased expression of the laminin receptor in human colon cancer.
Cioce, V.; Castronovo, Vincenzo ULg; Shmookler, B. M. et al

in Journal of the National Cancer Institute (1991), 83(1), 29-36

It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis ... [more ▼]

It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis. In this study, the expression of laminin-receptor-precursor messenger RNA (mRNA) and 67-kd protein was analyzed in human colon adenocarcinoma. In 22 of 23 patients with colon cancer, we found a 2- to 23-fold increase in levels of laminin-receptor-precursor mRNA in the cancer tissues compared with those in matched normal adjacent colonic mucosa. In 10 of 11 cases studied, the level of 67-kd laminin receptor, detected by affinity-purified anti-laminin-receptor synthetic peptide antibodies on immunoblots of matched tumor and normal tissue extracts, was higher in the colon carcinoma tissue. Immunodetection of laminin receptor in tissue sections using anti-laminin-receptor-peptide antibodies confirmed that the increased expression of laminin receptor was specifically associated with the cancer cells. In a series of 72 paraffin sections of colon lesions, we observed a correlation between the expression of the laminin receptor and the Dukes' classification. Our observations indicate that increased expression of laminin-receptor-precursor mRNA is associated with enhanced levels of the 67-kd laminin receptor as well as with the invasive phenotype of colon carcinoma. Detection of this metastasis-associated gene product may be a valuable adjunct in the evaluation of human colon cancer. [less ▲]

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See detailRésection ureteroscopique du polype fibreux de l'uretère
Nicolas, Hubert; Vandeberg, Colette ULg; Bonnet, Pierre ULg et al

in Acta Urologica Belgica (1991), 59(3), 119-30

From a new case of fibroepithelial polyp in a 41 year old woman, we reviewed literature on this rare and benign pathology, for which differential diagnosis with malignant tumor is most often obtained ... [more ▼]

From a new case of fibroepithelial polyp in a 41 year old woman, we reviewed literature on this rare and benign pathology, for which differential diagnosis with malignant tumor is most often obtained surgically. We emphasize both diagnostic and therapeutic advantages of endoscopic approach of this lesion. [less ▲]

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See detailL'immersion dans la vie professionnelle dès la formation initiale : pourquoi ?
Beckers, Jacqueline ULg

in Revue de la Direction Générale de l’Organisation des Etudes (1991), 2

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See detailA "zinc finger-like" domain in the 54KDA protein of several pestiviruses
De Moerlooze, L.; Renard, A.; Lecomte, C. et al

in Archives of Virology. Supplementum (1991), 3

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See detailCharacterization of a set of variable number of tandem repeat markers conserved in Bovidae
Georges, Michel ULg; Gunawardana, A.; Threadgill, D. et al

in Genomics (1991), 11(1), 24-32

Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American ... [more ▼]

Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae [less ▲]

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See detailStructure du [4-phénylacétylimino-1-(1,2,4-triazolio)]acétate de sodium dihydrate
Dupont, L.; Englebert, S.; Dideberg, O. et al

in Acta Crystallographica (1991), C47

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