References of "Article"
     in
Bookmark and Share    
See detailLes constellations (IV).
Renson, Pierre ULg

in Ciel et Terre (1997), 113

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailLa néphropathie épidémique a Hantavirus
Weekers, Laurent ULg; Biessaux, Yves ULg; Lamproye, Anne ULg et al

in Revue Médicale de Liège (1997), 52(8), 515-519

L'Entre-Sambre-et-Meuse a été le siège d'une épidémie de néphropathie épidémique à Hantavirus (NE) en 199293. Cinq cas ont été rencontrés au CHU de Liège en moins d'un an. Le virus responsable de cette ... [more ▼]

L'Entre-Sambre-et-Meuse a été le siège d'une épidémie de néphropathie épidémique à Hantavirus (NE) en 199293. Cinq cas ont été rencontrés au CHU de Liège en moins d'un an. Le virus responsable de cette affection appartient à la famille dès Hantavirus dont il existe huit sérotypes aux caractéristiques propres en terme de vecteur (rongeurs), de distribution géographique et de pathogénicité. Les pathologies induites chez l'homme vont de la néphropathie épidémique à Hantavirus - d'évolution le plus souvent bénigne- au syndrome pulmonaire à Hantavirus (SPH) - fréquemment mortel - en passant par la fièvre hémorragique avec syndrome rénal (FHSR) de sévérité intermédiaire. Dans nos contrées, on rencontre le sérotype Puumala dont le vecteur est le campagnol roussâtre. L'homme s'infecte par inhalation de particules contaminées et développe dans un pourcentage non précisé des cas, après une période d'incubation de 1 à 3 semaines, un tableau clinique de néphropathie épidémique à Hantavirus. Celui-ci se caractérise par l'apparition brutale d'une fièvre, de myalgies diffuses, de douleurs abdominales et/ou lombaires et de céphalées. Apparaissent ensuite à des degrés divers: nausées et vomissements, oligurie, myopie aiguë, toux,diathèse hémorragique, diarrhée... Biologiquement, on observe l'association d'une insuffisance rénale aiguë et d'une thrombopénie. L'évolution est spontanément favorable dans les deux à trois semaines. Le diagnostic suggéré par la clinique sera confirmé par la sérologie. La physiopathologie de l'insuffisance rénale et de la thrombopénie fait intervenir des interactions complexes entre l'hôte et le virus. Il n'existe pas de thérapeutique spécifique pour la néphropathie épidémique à Hantavirus. Les formes plus sévères (SPH et FHSR) peuvent bénéficierd'un traitement par Ribavirineen iv. [less ▲]

Detailed reference viewed: 93 (4 ULg)
See detailEsquisse d'un bilan provisoire
Beckers, Jacqueline ULg; Motte, André

in Puzzle (1997), 4

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailThrombolysis of Occluded Infrainguinal Bypass Grafts
VAN DAMME, Hendrik ULg; TROTTEUR, Geneviève ULg; Dongelinger, R.F. et al

in Acta Chirurgica Belgica (1997), 97(4), 177-83

From January 1993 to December 1995, intraarterial catheter guided urokinase infusion was used as an initial approach in the management of 29 episodes of infrainguinal graft thrombosis (12 venous and 17 ... [more ▼]

From January 1993 to December 1995, intraarterial catheter guided urokinase infusion was used as an initial approach in the management of 29 episodes of infrainguinal graft thrombosis (12 venous and 17 prosthetic grafts) in 27 patients. The infusion catheter was embedded inside the occluding clot which was infiltrated by 225.000 U urokinase from distal to proximal. Local low-dose urokinase (1.000 U/kg/hr) was continued for a mean of 39 hours. By this regimen, prompt relief of ischaemia was achieved in 69% (20/29) of cases. Complete recanalization was obtained in 79% of cases. In six cases, the graft remained totally (n = 3) or partially (n = 3) occluded. Two of these patients benefited from secondary surgery, two improved clinically by conservative treatment, and two required amputation. In the 23 successful cases, thrombolysis unmasked an underlying flow-limiting stenosis in 83% (19/23), that was subsequently corrected by percutaneous balloon angioplasty (n = 15), by surgery (n = 3), or by a combination of both (n = 4). One early rethrombosis resulted in an amputation. The immediate limb-salvage rate was 89% (26/29). Surgical intervention was avoided in 17 cases (58%). The main hospital stay was 13 days. The short-term follow-up (mean of 17 months) reveals a high early rethrombosis rate (8/23 or 35%) within one year. Four of these repeated graft failures evolved to amputation. At one year, the overall limb salvage rate dropped to 79%. Thrombolytic management of infrainguinal occluded bypass grafts gives excellent initial technical results (79%), minimizing the need for major surgical revision. It is however characterized by a high procedure-related morbidity (21%). These immediate favourable results are not longstanding. Diffuse graft disease, limited outflow and high recurrence rate of anastomotic stenoses after balloon angioplasty explain poor long-term results after thrombolysis of failed grafts. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailEffect of platelet-activating factor on bovine pulmonary function
Van de Weerdt, ML; Desmecht, Daniel ULg; Lekeux, Pierre ULg

in Veterinary Record : Journal of the British Veterinary Association (1997), 141(7), 168-172

The objectives of this study were to determine whether the infusion of platelet-activating factor would modify bovine pulmonary function, heart rate and platelet count and whether any such modifications ... [more ▼]

The objectives of this study were to determine whether the infusion of platelet-activating factor would modify bovine pulmonary function, heart rate and platelet count and whether any such modifications could be antagonised by the prior intravenous injection of a specific antagonist (WEB 2086). In saline-pretreated calves, the respiratory rate, total lung resistance (RL) and maximal changes in transpulmonary pressure (delta Ptp) were significantly increased whereas lung dynamic compliance (CLdyn), tidal volume (VT), platelet count and heart rate were significantly decreased. The changes in RL, CLdyn, VT and delta Ptp were abolished by pretreatment with WEB 2086, whereas respiratory rate remained significantly increased to 125 per cent of the baseline value, but less than in the saline-pretreated calves in which it was 250 per cent of the baseline value. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailDifferential expression of galectin-1 and galectin-3 during first trimester human embryogenesis.
van den Brûle, Frédéric; Fernandez, Pedro L.; Buicu, Crina et al

in Developmental Dynamics : An Official Publication of the American Association of Anatomists (1997), 209(4), 399-405

Development of complex organisms requires specific temporospatial differentiation and expression of the correct phenotype through activation of a variety of genes. Galectins are mammalian lectins able to ... [more ▼]

Development of complex organisms requires specific temporospatial differentiation and expression of the correct phenotype through activation of a variety of genes. Galectins are mammalian lectins able to interact with various extracellular matrix glycoconjugates and have been implicated in several biological events including cell attachment, differentiation, apoptosis, embryogenesis, and cancer invasion and metastasis. In this study, we have examined the expression of galectin-1 and galectin-3 during human first trimester embryogenesis using immunohistochemistry and Western blotting. Variable amounts of galectin-1 and galectin-3 were detected in all tissue protein extracts. Galectin-1 expression was demonstrated in the connective tissue and derived tissues such as smooth and striated muscle cells, and in some epithelia, such as in the basal layers of the skin after 14 weeks and in the epithelial cells of the gonads. Galectin-3 was detected mainly in epithelia, such as the skin, epithelial lining of the digestive and respiratory tract, and urothelium and excretory tubes of the kidney, but also in the myocardial cells, in the peripheral and preossifying hypertrophic chondrocytes, and in the notochord and in the liver. Our study constitutes the first demonstration of galectin-1 and galectin-3 during human embryogenesis. The differential expression of these two lectins suggests that they could participate in the complex processes of tissue differentiation. [less ▲]

Detailed reference viewed: 61 (23 ULg)
Full Text
Peer Reviewed
See detailAnalysis of the Biochemical Properties of, and Complex Formation between, Glycoproteins H and L of the Gamma2 Herpesvirus Bovine Herpesvirus-4
Lomonte, P.; Filée, Patrice ULg; Lyaku, J. R. et al

in Journal of General Virology (The) (1997), 78((Pt 8)), 2015-23

Genes encoding glycoprotein gH and gL homologues were localized in the genome of the gamma-herpesvirus bovine herpesvirus-4 (BHV-4). Both genes were sequenced and glutathione S-transferase fusion proteins ... [more ▼]

Genes encoding glycoprotein gH and gL homologues were localized in the genome of the gamma-herpesvirus bovine herpesvirus-4 (BHV-4). Both genes were sequenced and glutathione S-transferase fusion proteins were produced and used to immunize rabbits against the translation products of the two genes. The anti-gH serum recognized a protein with an apparent molecular mass (MM) of 110 kDa both in infected cells and in virions. This protein was sensitive to endo-beta-N-acetylglucosaminase-H (endoH) and endoglycosidase F-N-glycosidase F (endoF-PNGaseF) digestion. A protein with the same relative mobility was immunoprecipitated from infected cells radiolabelled with [3H]glucosamine which confirmed that this product (gp110), now designated BHV-4 gH, was glycosylated. Western blotting with the anti-gL serum detected in infected cells a product with an apparent MM ranging from 31-35 kDa and diffusely migrating protein species ranging from 45-65 kDa. Tunicamycin, monensin, endoH or endoF-PNGaseF treatments showed that both the 31-35 kDa and the 45-65 kDa proteins were glycosylated, gp31-35 being a precursor of the 45-65 kDa glycoprotein species. In radioimmunoprecipitation assays, the anti-gL serum immunoprecipitated from infected cells two glycosylated proteins with apparent MMs of 31-35 kDa (gp31-35) and 45-55 kDa (gp45-55). However a third glycoprotein, gp110, was also immunoprecipitated together with gp31-35 and gp45-55. gp110 and gp45-55 were subsequently confirmed to be virion glycoproteins corresponding to mature forms of BHV-4 gH and gL respectively. In addition, the present study clearly demonstrated complex formation between BHV-4 gH and gL both in virions and in infected cells. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailSynthesis and characterization of hydrogenated poly[alkylmethacrylate(-b-styrene)-b-butadiene-b-(styrene-b-) alkylmethacrylate] triblock and pentablock copolymers
Yu, Jian Ming; Yu, Yisong; Dubois, Philippe ULg et al

in Polymer (1997), 38(12), 3091-3101

Triblock and pentablock copolymers of the X(Y)B(Y)X type have been synthesized by the sequential living anionic polymerization of butadiene (B), styrene (Y) and alkylmethacrylate (X), respectively. The ... [more ▼]

Triblock and pentablock copolymers of the X(Y)B(Y)X type have been synthesized by the sequential living anionic polymerization of butadiene (B), styrene (Y) and alkylmethacrylate (X), respectively. The diadduct of t-BuLi onto m-diisopropenylbenzene (m-DIB) has been used as a difunctional initiator. Methylmethacrylate (MMA), t-butylmethacrylate (tBMA) and isobornylmethacrylate (IBMA) have been used as precursors of the outerblocks X. The polybutadiene (PBD) midblock that contains ca 42-45% 1,2-units has been selectively hydrogenated into a saturated poly(ethylene-co-1-butene) (PEB) block. The homogeneous hydrogenation catalysis has no deleterious effect on the copolymer integrity. These completely soluble thermoplastic elastomers have been characterized by FT i.r., n.m.r., d.s.c. and d.m.a. The PEB midblock has a low Tg (-50°C) and a small propensity to crystallize. The effect of hydrogenation on the morphology and mechanical properties depends on the outer block. Upon hydrogenation of the PBD midblock in polymethylmethacrylate (PMMA) and polyisobornylmethacrylate (PIBMA) containing triblock copolymers, the ultimate tensile strength is increased (except for a hard block content > 50%) due to a sharper phase separation, whereas the elongation at break is decreased. The extent of phase separation is reduced in polyt-butylmethacrylate(PtBMA) containing triblock copolymers upon hydrogenation and the ultimate tensile strength is slightly decreased. Stereocomplexation of the syndiotactic PMMA outerblocks is observed to occur upon blending with isotactic PMMA [less ▲]

Detailed reference viewed: 48 (13 ULg)
Full Text
Peer Reviewed
See detailConsequences of Cold and Warm Ischemia on Pulmonary Mitochondrial Respiratory Function
Detry, Olivier ULg; Willet, K.; Lambermont, Bernard ULg et al

in Transplantation Proceedings (1997), 29(5), 2338-9

IN RECENT years, pulmonary transplantation has become the treatment of choice for several end-stage lung diseases, but remains limited by the scarcity of suitable donors and the lack of reliable prolonged ... [more ▼]

IN RECENT years, pulmonary transplantation has become the treatment of choice for several end-stage lung diseases, but remains limited by the scarcity of suitable donors and the lack of reliable prolonged method of lung preservation.1 Transplantation of lung 6 hours postharvest leads to an increased incidence of primary graft dysfunction, due in part to ischemic damage of pulmonary cell structure and metabolism, and to acute reperfusion injury. However, very little is known about the real mechanisms of pulmonary cell injuries before, during, and after lung transplantation. <br /> <br />During ischemia, the cytosolic and mitochondrial adenine nucleotide content falls,2,3 phospholipids are degraded, membrane permeabilities are increased, and the cytosolic levels of Na+, Ca2+ and phosphate are raised.4 Thus, cold and warm ischemia may induce cell dysfunctions and irreversible injuries responsible for necrosis. As mitochondia are believed to be the site of the determinants of irreversibility,5 the study of permanent oxidative phosphorylation damage after ischemia should be of great interest. <br /> <br />The aim of this present study was to investigate the consequences of warm and cold ischemia on the oxidative phosphorylation of isolated lung mitochondria [less ▲]

Detailed reference viewed: 31 (9 ULg)
Full Text
Peer Reviewed
See detailBiodegradable and biocompatible inorganic-organic hybrid materials. 1. Synthesis and characterization
Tian, Dong; Dubois, Philippe ULg; Jérôme, Robert ULg

in Journal of Polymer Science. Part A, Polymer Chemistry (1997), 35(11), 2295-2309

The hydroxyl or vinyl end-groups of linear or three-arm star-shaped poly(-caprolactone) (PCL) chains have been derivatized into triethoxysilane groups reactive in the sol-gel process. New transparent ... [more ▼]

The hydroxyl or vinyl end-groups of linear or three-arm star-shaped poly(-caprolactone) (PCL) chains have been derivatized into triethoxysilane groups reactive in the sol-gel process. New transparent hybrid materials that combine tetraethylorthosilicate (TEOS) and PCL known for biodegradability and biocompatibility have accordingly been prepared. The sol-gel process is, however, limited by the early vitrification of the reactive system. However, thermal posttreatment can overcome these diffusional and/or kinetic limitations as assessed by a set of analytical methods. The thermal stability of PCL is improved by incorporation into the silica network. Conversely, the thermal stability of the ceramer depends on the effective PCL content. The extent of PCL incorporation into the silica network depends on PCL molecular weight, number, and reactivity of the PCL functional groups. IR spectroscopy has shown that hydrogen bonding occurs between the ester groups of PCL and residual OH groups of the silicate component. © 1997 John Wiley [less ▲]

Detailed reference viewed: 165 (9 ULg)
Peer Reviewed
See detailInsuffisance rénale, syndrome inflammatoire et anévrysme de l'aorte abdominale ... où est le lien?
Vazquez, C.; Bonnet, Pierre ULg; Fastre, M. et al

in Revue Médicale de Liège (1997), 52(8), 511-4

Detailed reference viewed: 28 (0 ULg)
Full Text
Peer Reviewed
See detailSuccessful Controlled Limb Reperfusion after Severe Prolonged Ischemia
Defraigne, Jean-Olivier ULg; Pincemail, Joël ULg; Laroche, C. et al

in Journal of Vascular Surgery : Official Publication, the Society for Vascular Surgery and International Society For Cardiovascular Surgery, North American Chapter (1997), 26(2), 346-50

Controlled limb reperfusion was performed in two patients who were admitted for lower limb-threatening ischemia as a result of embolism. After embolectomy, the inflow blood was drained with a cannula and ... [more ▼]

Controlled limb reperfusion was performed in two patients who were admitted for lower limb-threatening ischemia as a result of embolism. After embolectomy, the inflow blood was drained with a cannula and mixed with a crystalloid solution to obtain an hyperosmolar, hypocalcemic, alkalotic, and substrate-enriched (aspartate, glutamate) reperfusate. This reperfusate was reinjected with a roller pump for 30 minutes through two cannulas inserted in the profunda and superficial femoral arteries. Temperature, intraarterial pressures, and flow were closely controlled. No complications occurred, and complete recovery of motor and sensory functions were observed, with restoration of pedal pulses. [less ▲]

Detailed reference viewed: 161 (4 ULg)
Full Text
Peer Reviewed
See detailCoupling of α,ω-dichloropoly (methylphenylsilane) and living poly (styryllithium). Study of the coupling reaction
Demoustier-Champagne, Sophie; Canivet, I.; Devaux, Jacques et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1997), 35(10), 1939-1948

Both di- and triblock copolymers consisting of polystyrene (PS) in conjunction with poly(methylphenylsilane) (PMPS) have been successfully prepared by coupling of ,-dichloro-PMPS with poly(styryllithium ... [more ▼]

Both di- and triblock copolymers consisting of polystyrene (PS) in conjunction with poly(methylphenylsilane) (PMPS) have been successfully prepared by coupling of ,-dichloro-PMPS with poly(styryllithium). The study of this reaction has shown a sudden limitation of the coupling yield, which however depends on the PS block length. Both the polymer concentration and the solvent have also an effect on the coupling reaction. Morphology of the PS-PMPS block copolymers has been observed by transmission electron microscopy. Very complex morphologies have been reported more likely as the result of the competition between the phase separation induced by PS and PMPS immiscibility and the tendency of PMPS to form ordered structures. [less ▲]

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailLiving anionic homo- and block copolymerization of 2-(tert-butylamino) ethyl methacrylate
Creutz, Serge; Teyssié, Philippe; Jérôme, Robert ULg

in Journal of Polymer Science. Part A, Polymer Chemistry (1997), 35(10), 2035-2040

Anionic polymerization of 2-(tert-butylamino)ethyl methacrylate (tBAEMA), which bears an unprotected secondary amine moiety, has been investigated in THF at -78°C. The presence of lithium chloride has ... [more ▼]

Anionic polymerization of 2-(tert-butylamino)ethyl methacrylate (tBAEMA), which bears an unprotected secondary amine moiety, has been investigated in THF at -78°C. The presence of lithium chloride has been shown to be desirable to afford narrow molecular weight distribution as well as a good agreement between theoretical and observed molecular weight. The living character of the polymerization has also been demonstrated, and the synthesis of block copolymers carried out successfully. They have been analyzed by SEC by adding a mixture of secondary and tertiary amines to the eluent (THF) so as to avoid any polymer adsorption during elution. © 1997 John Wiley [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailDifunctional initiator based on 1,3-diisopropenylbenzene. 6. Synthesis of methyl methacrylate-butadiene-methyl methacrylate triblock copolymers
Yu, Y. S.; Dubois, Philippe ULg; Teyssié, Philippe et al

in Macromolecules (1997), 30(15), 4254-4261

Synthesis of PMMA−PBD−PMMA (MBM) triblock copolymers with a 1,3-diisopropenylbenzene- (DIB-) based initiator is studied. Diethyl ether, tert-butyl methyl ether, N,N,N‘,N‘-tetramethylethylenediamine (TMEDA ... [more ▼]

Synthesis of PMMA−PBD−PMMA (MBM) triblock copolymers with a 1,3-diisopropenylbenzene- (DIB-) based initiator is studied. Diethyl ether, tert-butyl methyl ether, N,N,N‘,N‘-tetramethylethylenediamine (TMEDA) and THF were found to be efficient polar additives for obtaining PMMA−PBD−PMMA triblock copolymers, but these triblock copolymers displayed a high 1,2-microstructure of the PBD block. A combination of the initiator seeding technique and weakly polar additives has allowed MBM triblock copolymers with high 1,4-microstructure of PBD to be obtained. These copolymers exhibited a definitely higher upper service temperature than conventional thermoplastic elastomers, i.e. SBS or SIS Kratons. The influence of chain microstructure, copolymer composition and block length on the mechanical properties has been studied. Short polystyrene blocks of ca. Mn 2000 have been incorporated in between the PBD central block and the PMMA outer sequences. A higher tensile strength was observed for these MBM-like triblock coplymers. Contamination by a diblock structure expectedly had a very depressive effect on the bulk properties, and the stress−strain behavior mainly depended on the PMMA content and secondarily on molecular weight. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailExchange of polymer molecules between block copolymer micelles studied by emission spectroscopy. A method for the quantification of unimer exchange rates
Creutz, Serge; van Stam, Jan; Antoun, Sayed et al

in Macromolecules (1997), 30(14), 4078-4083

Amphiphilic block copolymers, composed of poly(sodium methacrylate) (PMANa) as the water soluble sequence and poly((dimethylamino)ethyl methacrylate) (PDMAEMA) as the hydrophobic sequence, were ... [more ▼]

Amphiphilic block copolymers, composed of poly(sodium methacrylate) (PMANa) as the water soluble sequence and poly((dimethylamino)ethyl methacrylate) (PDMAEMA) as the hydrophobic sequence, were anionically synthesized. Due to their amphiphilic character, these polymers aggregate into micelles in aqueous solutions. Of particular interest is the possibility of exchange of free chains, called unimers, between micelles and the bulk and the quantification of the exchange rate. The exchange of unimers between the micellar aggregates was determined spectroscopically. A donor molecule, naphthalene, was covalently bound to the hydrophobic blocks of the copolymers, while the acceptor, pyrene, was dissolved in aggregates built up from an identical, but unlabeled, copolymer. The energy transfer from an excited donor to a ground-state acceptor, with subsequent emission from the acceptor, was used to follow the migration of donor-labeled chains into micelles containing the dissolved acceptor. These data were analyzed according to a kinetic model, and the respective kinetic parameters could be determined. The influence of the relative composition of the block copolymer on the exchange rate was investigated to illustrate the usability of the kinetic model. While keeping the relative amount of comonomers as well as the molecular weights constant, the molecular architecture was also studied: hence the diblock (PDMAEMA-b-PMANa) and the corresponding triblocks (PDMAEMA-b-PMANa-b-PDMAEMA) and (PMANa-b-PDMAEMA-b-PMANa) were investigated. [less ▲]

Detailed reference viewed: 33 (1 ULg)
Full Text
Peer Reviewed
See detailDesign of polymer blend rheology: 4. Effect of polymethacrylic ionomers on the melt viscosity of polyamide m x D,6
Degée, Philippe; Vankan, R.; Teyssié, Philippe et al

in Polymer (1997), 38(15), 3861-3867

Rheological properties of blends of polyamide m x D,6 (PA) with random copolymers of methyl methacrylate and methacrylic acid (P(MMA-co-MAA)) and the related ionomers (P(MMA-co-metal MA)), respectively ... [more ▼]

Rheological properties of blends of polyamide m x D,6 (PA) with random copolymers of methyl methacrylate and methacrylic acid (P(MMA-co-MAA)) and the related ionomers (P(MMA-co-metal MA)), respectively, have been investigated at 260°C. Addition of small amounts of suitable ionomers (up to 5 wt%) remarkably increases the melt viscosity of PA. At low shear rates and depending on the metal cation of the ionomer, the rheofluidity of PA is reduced by at least two orders of magnitude. The PA melt viscosity increases with the cation as follows: Ni2+ < Ba2+ < Mg2+ < Li+ < Na+ < K+ < Cs+. The major effect is believed to occur when the polymer compatibility is improved to the point where a finely dispersed two-phase system with a low interfacial tension is reached, as a result of strong ion-dipole interactions. Polyblends show a rheothinning behaviour which agrees with the reversibility of H-bonding and dipolar interactions. [less ▲]

Detailed reference viewed: 38 (2 ULg)
Full Text
See detailArt, Mail-art, “Médiart”
Durand, Pascal ULg

in Cyber-Flux-News (1997)

Detailed reference viewed: 132 (1 ULg)
Full Text
Peer Reviewed
See detailVasotocinergic Innervation of Areas Containing Aromatase-Immunoreactive Cells in the Quail Forebrain
Balthazart, Jacques ULg; Absil, Philippe ULg; Viglietti-Panzica, C. et al

in Journal of Neurobiology (1997), 33(1), 45-60

In the male quail forebrain, aromatase-immunoreactive (ARO-ir) elements are clustered within the sexually dimorphic medial preoptic nucleus (POM), nucleus striae terminalis (nST), nucleus accumbens (nAc ... [more ▼]

In the male quail forebrain, aromatase-immunoreactive (ARO-ir) elements are clustered within the sexually dimorphic medial preoptic nucleus (POM), nucleus striae terminalis (nST), nucleus accumbens (nAc), and ventromedial and tuberal hypothalamus. These ARO-ir cells are sensitive to testosterone and its metabolites: Their number and size increase after exposure to these steroids. The POM and lateral septum are also characterized by a dense vasotocinergic innervation that is also sensitive to testosterone. We analyzed here the anatomical relationships between ARO-ir elements and VT-ir fibers in the quail prosencephalon. Sequential staining for vasotocin, aromatase, or vasotocin plus aromatase was performed on adjacent 30-microm-thick cryostat sections. High concentrations of thin VT-ir fibers were observed within the POM, nST, lateral septum, periventricular mesencephalic central gray, and ventromedial and tuberal hypothalamus. There was a close correspondence between the extension of the ARO-ir cells and of VT-ir fibers. In double-labeled sections, all clusters of ARO-ir cells with the exception of those located in the nAc were embedded in a dense network of VT-ir fibers. Many of the VT-ir terminals appeared to end in the neuropile surrounding ARO-ir elements rather than directly on their cell bodies. This study supports the idea that the testosterone-dependent aromatase system is directly innervated by a testosterone-dependent peptidergic system. Aromatase-containing cells could therefore be modulated by steroids both directly and indirectly through the vasotocin system. Alternatively, this neuroanatomical arrangement may mediate the control of vasotocin synthesis or release by steroids. Functional studies demonstrate that both aromatase and vasotocin affect reproductive behavior in quail, and the present data provide anatomical support for the integration of these effects. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailDual Multimodular Class a Penicillin-Binding Proteins in Mycobacterium Leprae
Lepage, Sophie; Dubois, Philippe ULg; Ghosh, Thushar-Kanti et al

in Journal of Bacteriology (1997), 179(14), 4627-4630

The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been ... [more ▼]

The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity. [less ▲]

Detailed reference viewed: 16 (7 ULg)
Full Text
Peer Reviewed
See detailEfficiency of T Cell Triggering by Anti-Cd3 Monoclonal Antibodies (Mab) with Potential Usefulness in Bispecific Mab Generation
Jacobs, Nathalie ULg; Mazzoni, A.; Mezzanzanica, D. et al

in Cancer Immunology, Immunotherapy (1997), 44(5), 257-64

T cell triggering can be achieved by monoclonal antibodies (mAbs) specific for the CD3/TcR complex. In the presence of appropriate costimulation and/or progression factors, such triggering permits the ... [more ▼]

T cell triggering can be achieved by monoclonal antibodies (mAbs) specific for the CD3/TcR complex. In the presence of appropriate costimulation and/or progression factors, such triggering permits the generation of effector cells for immunotherapy protocols involving the redirection of T cell lysis against tumor cells by mAbs bispecific for anti-CD3/anti-tumor cells (bs-mAbs). Focusing our analysis on the clinically relevant bs-mAb OC/TR, we found that bs-mAbs generated with the same anti tumor specificity, but two other anti-CD3 mAbs, TR66 and OKT3, have the same and a significantly lower lytic potential, respectively, compared with that of OC/TR. To evaluate the relevance of the anti-CD3 component, we examined several anti-CD3 mAbs with respect to binding parameters and the ability to trigger T lymphocytes. Competitive binding assays suggested that all anti-CD3 mAbs recognized the same or overlapping epitopes, although mAbs BMA030 and OC/TR bound with lower avidity than did alpha CD3 (the bivalent anti-CD3 mAb produced by the hybrid hybridoma OC/TR). TR66 and OKT3, as determined by measurement of the affinity constants. In all lymphocyte populations examined, which included resting peripheral blood mononuclear cells (PBMC), activated PBMC and T cell clones, OKT3, BMA033 and OC/TR failed to mobilize Ca2+ without cross-linking, whereas alpha CD3, in both murine and murine-human chimeric versions, TR66 and BMA030, did not require cross-linking. The ability to induce CD3 modulation was associated in part with the induction of Ca2+ fluxes. Despite the differences in the behavior of these mAbs in triggering the events that precede proliferation, all of them ultimately led to expression of the IL-2 receptor and to proliferation in T cells in the presence of accessory cells. Our data suggest that anti-CD3 mAbs that bind more rapidly (strong Ca2+ mobilizers) and more tightly under physiological conditions are good candidates for retargeting T cells in the bs-mAb clinical application. [less ▲]

Detailed reference viewed: 39 (5 ULg)
Full Text
Peer Reviewed
See detailChronic varicella-zoster virus skin lesions in patients with human immunodeficiency virus are related to decreased expression of gE and gB
Nikkels, Arjen ULg; Rentier, Bernard ULg; Pierard, Gérald ULg

in Journal of Infectious Diseases (1997), 176(1), 261-264

The pathogenesis of chronic, verrucous varicella-zoster virus (VZV) cutaneous lesions in human immunodeficiency virus (HIV)-infected persons is unknown. It has been hypothesized that these lesions are due ... [more ▼]

The pathogenesis of chronic, verrucous varicella-zoster virus (VZV) cutaneous lesions in human immunodeficiency virus (HIV)-infected persons is unknown. It has been hypothesized that these lesions are due to an altered pattern of virus gene expression. Immediate early and late (L) gene expression in five chronic verrucous VZV lesions, four full-blown herpes zoster vesicular lesions in HIV-infected persons, and eight vesicular herpes zoster lesions in immunocompetent individuals was semiquantitatively assessed immunohistochemically using specific antibodies to the IE63, gE (L), and gB (L) proteins. All patients had evidence of IE63 expression in keratinocytes; however, gE expression was either weak or absent in keratinocytes of three verrucous lesions, and gB was either weak or absent in two. These results suggest that chronic VZV skin lesions are associated with diminished gE and gB expression. It is inferred that the VZV behavior in keratinocytes may vary from a latency-like state to a fully developed, productive infection. [less ▲]

Detailed reference viewed: 47 (5 ULg)
Peer Reviewed
See detailDétection de la viabilité myocardique: rôle de l'échocardiographie de stress
Pierard, Luc ULg; Hoffer, E.

in Archives des Maladies du Coeur et des Vaisseaux (1997), 90(Spec No 4), 3-16

Identification of myocardial viability is of great clinical importance in patients with acute coronary events or chronic coronary artery disease. Stress echocardiography has emerged as a new accurate and ... [more ▼]

Identification of myocardial viability is of great clinical importance in patients with acute coronary events or chronic coronary artery disease. Stress echocardiography has emerged as a new accurate and cost-effective imaging modality to detect contractile reserve. Numerous studies have recently been published in this field. This article summarizes the main findings of these investigations and presents practical aspects of the method. [less ▲]

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailLe contrôle des juridictions de fond sur la désignation par procureur général du magistrat compétent en application de l’article 155, alinéa 2, du Code judiciaire.
Michiels, Olivier ULg

in Journal des Tribunaux (1997), 5849

L’arrêt prononcé par la Cour de cassation le 8 octobre 1996 permet d’apprécier l’étendue du contrôle que peuvent exercer les juridictions de jugement sur la compétence de l’organe du ministère public ... [more ▼]

L’arrêt prononcé par la Cour de cassation le 8 octobre 1996 permet d’apprécier l’étendue du contrôle que peuvent exercer les juridictions de jugement sur la compétence de l’organe du ministère public amené à engager des poursuites pénales en cas de concours ou de connexité entre des infractions qui relèvent de la compétence des juridictions du travail et des infractions de droit commun. [less ▲]

Detailed reference viewed: 25 (1 ULg)
Full Text
Peer Reviewed
See detailApports et perspectives des dérivés de l'hémoglobine
Remy, Bernadette ULg; Deby, Ginette ULg; Lamy, Maurice ULg

in Schweizerische Medizinische Wochenschrift (1997), 127(25), 1088-96

Anoxia-reoxygenation leads to severe metabolic alterations, which result in a generalized inflammatory reaction and multiple organ dysfunction. Direct blood transfusion limits these alterations, but is ... [more ▼]

Anoxia-reoxygenation leads to severe metabolic alterations, which result in a generalized inflammatory reaction and multiple organ dysfunction. Direct blood transfusion limits these alterations, but is accompanied by risk of transmission of infections or viral diseases. To avoid these risks, "blood substitutes" have been designed. The modified hemoglobins are not true blood substitutes because they do not possess the complex functions of erythrocytes. They are only oxygen carriers, with a short intravascular life, adapted for temporary use. They are stable, devoid of toxicity and antigenicity, and are able to carry and deliver O2 without regulation of this oxygen transport and without chemical reaction with O2. They possess rheologic properties and an oncotic pressure like those of blood. The use of natural hemoglobin solutions, obtained after lysis of erythrocytes, remains "at risk" because these solutions easily form methemoglobin, increase the oncotic pressure, present renal toxicity, and possess a too high affinity for O2. For these reasons, 5 types of modified hemoglobin solutions have been designed, prepared from human or bovine hemoglobin or by genetic engineering. These hemoglobins are highly purified to eliminate trace amounts of stroma, lipids and endotoxins, which are responsible for acute toxicity. They are modified by internal cross-linking between the monomers, or by binding to macromolecules. Afterwards, they can be polymerized or encapsulated in liposomes. The purpose of these modifications is to modulate the affinity for O2 (by decreasing the binding of O2 and increasing its delivery to tissue), to reduce the dissociation into monomers and to guard against oxidation into methemoglobin. Encapsulation in liposomes allows co-encapsulation of effector molecules and protective substances. Genetic engineering allows the production of recombinant hemoglobin with selective modifications. The modified hemoglobin solutions are essentially used in hemorrhagic shock and perioperative hemodilution. Experimental work in animals has afforded good results: restoration of normal O2 pressure and no toxicity. These assays allow frequent observation of an unexpected rapid hypertensive effect, transient, reversible, and that could be controlled by antihypertensive drugs. The mechanisms of this hypertensive effect remain controverted (stimulation of endothelin production, inhibition of nitric oxide effects, etc.). In humans, studies with healthy volunteers have been completed, while phase II clinical studies are under way in hypovolemic shock, in major abdominal, orthopedic and cardiac surgery, in stroke and in intensive care patients after surgery. The detailed results are awaited, but the modified hemoglobin solutions already appear to be without toxicity and present the same hypertensive effect as observed in animals. However, until now only low doses have been used, and the catabolism of these solutions remains largely unknown. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
Peer Reviewed
See detailPolarised light induced birefringence in azo dye doped polymer: a new model and polarised holographic experiments
Blanche, Pierre-Alexandre ULg; Lemaire, P. C.; Maertens, Christophe et al

in Optics Communications (1997), 139(1-3), 92-98

The photoinduced birefringence of azo dye DMNPAA (2,5-dimelthyl-4-(p-nitrophenylazo)anisole) in PVK (Poly(N-vinylcarbazole)) thin film is reported. A two-layer model of the film is proposed to take into ... [more ▼]

The photoinduced birefringence of azo dye DMNPAA (2,5-dimelthyl-4-(p-nitrophenylazo)anisole) in PVK (Poly(N-vinylcarbazole)) thin film is reported. A two-layer model of the film is proposed to take into account the different results obtained in photoinduced birefringence experiments. According to this model, the sample exhibits intrinsic birefringence but the absorbed light can induce orientation of the optically active molecules contained in the first layer of the film, lending to photoinduced birefringence. Holographic experiments have been carried out in this media with recording at 514 nm and readout at 633 nm. Diffraction efficiency versus polarisation shows that these polymers can record polarisation states as well as intensity patterns. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Peer Reviewed
See detailNeonatal and Preweanling Rats Are Able to Express Short-Term Behavioral Sensitization to Cocaine
Tirelli, Ezio ULg; Ferrara, Marie-Antoinette ULg

in European Journal of Pharmacology (1997), 328(2-3), 103-14

The present study assessed the ability of suckling rats to express short-term behavioral sensitization to cocaine prior to weaning. Rat pups, aged either 3, 5, 10, 12, 17 or 19 days at the beginning of ... [more ▼]

The present study assessed the ability of suckling rats to express short-term behavioral sensitization to cocaine prior to weaning. Rat pups, aged either 3, 5, 10, 12, 17 or 19 days at the beginning of the experiment, were placed in a chamber after daily injection with cocaine (7.5 or 15 mg/kg. i.p.) for either 2 or 4 consecutive days, and were tested for behavioral responsiveness to cocaine in the same chamber 24 h later (at either 7, 14 or 21 days of age). Such a short post-treatment interval was adopted, along with a consistent pairing of the testing context with the drug effect and a sensitive technique of behavioral measurement (video recording), in order to maximize the possibility of detecting any cocaine sensitization. Locomotion was sensitized at all ages, after both regimens in 14-day-old pups, but solely after 2 injections in 21- and 4 injections in 7-day-old pups. Sensitization was also expressed via behaviors specific to each age. Four cocaine injections augmented cocaine-induced uncoordinated movements of head, paws and body (horizontal activity) in 7-day-old pups, and mouth movements in 14-day-old pups. In 21-day-old pups, sensitization was dose- and regimen-dependently expressed via adult-like stereotyped head movements. In neonatal 7-day-old pups, cocaine sensitization was also visible as reductions in immobility (both injection regimens). Contrary to previous studies, these results indicate that, given the use of an appropriate methodology, short-term sensitization to the motoric effects of cocaine can be expressed by suckling rats prior to weaning, even after relatively short regimens of daily injections. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailIdentification of Catecholaminergic Inputs to and Outputs from Aromatase-Containing Brain Areas of the Japanese Quail by Tract Tracing Combined with Tyrosine Hydroxylase Immunocytochemistry
Balthazart, Jacques ULg; Absil, Philippe ULg

in Journal of Comparative Neurology (The) (1997), 382(3), 401-28

In the quail brain, aromatase-immunoreactive (ARO-ir) neurons located in the medial preoptic nucleus (POM) and caudal paleostriatum ventrale/nucleus accumbens/nucleus striae terminalis complex (PVT/nAc ... [more ▼]

In the quail brain, aromatase-immunoreactive (ARO-ir) neurons located in the medial preoptic nucleus (POM) and caudal paleostriatum ventrale/nucleus accumbens/nucleus striae terminalis complex (PVT/nAc/nST) receive catecholaminergic inputs identified by the presence of tyrosine hydroxylase-immunoreactive (TH-ir) fibers and punctate structures. The origin of these inputs was analyzed by retrograde tracing with cholera toxin B subunit (CTB) or red latex fluospheres (RLF) combined with TH immunocytochemistry. CTB and RLF injected in the POM or PVT/nAc/nST were found in cells located in anatomically discrete areas in the telencephalon (hippocampus, septum, archistriatum), hypothalamus (many areas in periventricular position), thalamus, mesencephalon, and pons. In these last two regions, many retrogradely labeled cells were located in dopaminergic areas such as the retroruberal field (RRF), substantia nigra (SN), and area ventralis of Tsai (AVT) but also in noradrenergic cell groups such as the locus ceruleus and subceruleus. CTB tracing showed that most of these connections are bidirectional. Many retrogradely labeled cells contained TH-ir material. As a mean, 10-20% and 40-60% of the RLF-containing cells in the dopaminergic areas were TH-ir when RLF had been injected in the POM or PVT/nAc/nST, respectively. TH-ir cells projecting to the POM appeared to be mostly located in the periventricular hypothalamus and in AVT, whereas projections to the PVT/nAc/nST originated mainly in the SN (with significant contributions from the RRF and AVT). These data support the existence of functional relationships between aromatase and catecholamines. [less ▲]

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailOptical Spectroscopy of EZ Canis Majoris: Indication for Large-Scale Structures in a Wolf-Rayet Wind
Morel, Thierry ULg; St-Louis, Nicole; Marchenko, Sergey V

in Astrophysical Journal (1997), 482

We have carried out optical spectroscopy of the Wolf-Rayet star EZ CMa during 20 consecutive nights in 1995 January in support of the IUE Mega-project. In parallel with this optical spectroscopy, we also ... [more ▼]

We have carried out optical spectroscopy of the Wolf-Rayet star EZ CMa during 20 consecutive nights in 1995 January in support of the IUE Mega-project. In parallel with this optical spectroscopy, we also monitored EZ CMa using narrowband photometry. The light curve was found to be remarkably stable when folded with the P=3.77 day period, and it had a peak-to-valley amplitude of 0.1 mag. The P Cygni absorption components of He I lambda 3889 and He I lambda 5876 display a similar global pattern of variability as was found for the simultaneously acquired UV profiles. The strengthening of the P Cygni absorption component of these transitions is associated with the maximum of the continuum flux. Conversely, the absorption trough of N V lambda 4604 gradually disappears as the star brightens. Although the emission parts of the lines are variable at different levels, they all show the same pattern of variability, which consists of phase-dependent shifts of extra emission components superposed on the profiles. A strong correlation is found between the continuum-light level and the equivalent width of most transitions. The line skewness and the full-width at half-maximum show a daily recurrence timescale, reflecting the light curve changes. We have addressed in a rigorous statistical way the significance of the variations by calculating the "temporal variance spectrum." For any given line, we found enhanced variability at some velocities, although the whole profile displays a statistically significant level of variability. Arguments against a compact companion as the cause of the observed periodic variability are presented. Instead, our observations strongly support the suggestion in the IUE Mega analysis that the atypical level of variability results from the rotation of a structured wind. We propose that the wind variability of EZ CMa is triggered by photospheric activity, or that the wind is controlled by a large-scale magnetic field. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Peer Reviewed
See detailComment on ``A model for meteoric magnesium in the ionosphere'' by W. J. McNeil, S. T. Lai, and E. Murad
Fesen, C. G.; Gérard, Jean-Claude ULg; Hays, P. B. et al

in Journal of Geophysical Research (1997), 102

Detailed reference viewed: 20 (0 ULg)
See detailLes constellation (III).
Renson, Pierre ULg

in Ciel et Terre (1997), 113

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailThe puzzling Luminous Blue Variable-like object HD 5980 in the Small Magellanic Cloud.
Heydari-Malayeri, M.; Rauw, Grégor ULg; Esslinger, O. et al

in Astronomy and Astrophysics (1997), 322

We have observed the exceptional SMC star HD 5980 during several runs from 1989 to 1995 at ESO La Silla. CASPEC at the 3.6m telescope and EMMI in echelle and long slit modes at NTT were used for ... [more ▼]

We have observed the exceptional SMC star HD 5980 during several runs from 1989 to 1995 at ESO La Silla. CASPEC at the 3.6m telescope and EMMI in echelle and long slit modes at NTT were used for spectroscopy. Sub-arcsecond images were obtained using SUSI at NTT and also an adaptive optics system at the 3.6m telescope. In all our spectra taken before 1994 September HD 5980 shows a spectral type of WN6. The 1994 September spectra were taken shortly after the maximum of the visual light-curve of the LBV-like phenomenon (Bateson & Jones, 1993PVSS...19...50B) and about one month before the observations of Barba et al. (1995ApJ...446L..23B). Near maximum visual brightness, HD 5980 displays a WN11-like spectrum with the HeI lines and the Balmer lines Hdelta and Hgamma showing well-developed PCyg profiles. The sub-arcsecond images (0.17" FWHM), through the near infrared bands J, H, and K, obtained in 1993 and 1996, show no stellar components down to 6.7 mag fainter than HD 5980 in K at a separation of 1.0" and the 3sigma level. For a separation of 0.3" this upper bound is 4.1mag fainter than HD 5980. The observed behavior of this object raises serious problems for our comprehension of the LBV phenomenon in the conventional scenarios of massive star evolution. The present observations cover a crucial period in the evolution of HD 5980 and will therefore be helpful for better understanding this peculiar object especially during its outburst as well as the evolution of W-R stars in general. [less ▲]

Detailed reference viewed: 128 (0 ULg)
Full Text
Peer Reviewed
See detailThe mean magnetic field modulus of AP stars
Mathys, G.; Hubrig, S.; Landstreet, J. D. et al

in Astronomy and Astrophysics. Supplement Series (1997), 123

We present new measurements of the mean magnetic field modulus of a sample of Ap stars with spectral lines resolved into magnetically split components. We report the discovery of 16 new stars having this ... [more ▼]

We present new measurements of the mean magnetic field modulus of a sample of Ap stars with spectral lines resolved into magnetically split components. We report the discovery of 16 new stars having this property. This brings the total number of such stars known to 42. We have performed more than 750 measurements of the mean field modulus of 40 of these 42 stars, between May 1988 and August 1995. The best of them have an estimated accuracy of 25 - 30 G. The availability of such a large number of measurements allows us to discuss for the first time the distribution of the field modulus intensities. A most intriguing result is the apparent existence of a sharp cutoff at the low end of this distribution, since no star with a field modulus (averaged over the rotation period) smaller than 2.8 kG has been found in this study. For more than one third of the studied stars, enough field determinations well distributed throughout the stellar rotation cycle have been achieved to allow us to characterize at least to some extent the variations of the field modulus. These variations are often significantly anharmonic, and it is not unusual for their extrema not to coincide in phase with the extrema of the longitudinal field (for the few stars for which enough data exist about the latter). This, together with considerations on the distribution of the relative amplitude of variation of the studied stars, supports the recently emerging evidence for markedly non-dipolar geometry and fine structure of the magnetic fields of most Ap stars. New or improved determinations of the rotation periods of 9 Ap stars have been achieved from the analysis of the variations of their mean magnetic field modulus. Tentative values of the period have been derived for 5 additional stars, and lower limits have been established for 10 stars. The shortest definite rotation period of an Ap star with magnetically resolved lines is 3.4 deg, while those stars that rotate slowest appear to have periods in excess of 70 or 75 years. As a result of this study, the number of known Ap stars with rotation periods longer than 30 days is almost doubled. We briefly rediscuss the slow-rotation tail of the period distribution of Ap stars. This study also yielded the discovery of radial velocity variations in 8 stars. There seems to be a deficiency of binaries with short orbital periods among Ap stars with magnetically resolved lines. Based on observations collected at the European Southern Observatory (La Silla, Chile; ESO programmes Nos. 43.7-004, 44.7-012, 49.7-030, 50.7-067, 51.7-041, 52.7-063, 53.7-028, 54.E-0416, and 55.E-0751), at the Observatoire de Haute-Provence (Saint-Michel-l'Observatoire, France), at Kitt Peak National Observatory, and at the Canada-France-Hawaii Telescope. Tables 2, 3, and 4 are also available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailEndogenous nitric oxide production and atrial natriuretic peptide biological activity in infants undergoing cardiac operations
Seghaye, Marie-Christine ULg; Duchateau, J.; Bruniaux, J. et al

in Critical Care Medicine (1997), 25(6), 1063-1070

Objectives: To examine whether preoperative heart failure end cardiac surgery influence nitric oxide production and atrial natriuretic peptide (ANP) biological activity in infants and whether nitric oxide ... [more ▼]

Objectives: To examine whether preoperative heart failure end cardiac surgery influence nitric oxide production and atrial natriuretic peptide (ANP) biological activity in infants and whether nitric oxide and ANP participate in the control of postoperative pulmonary vascular tone. Design: Prospective, clinical study. Setting: Tertiary pediatric cardiac intensive care unit in a referral cardiosurgical center. Patients: Nineteen infants (median age 4 months) undergoing cardiac surgery: 13 infants with ventricular or atrioventricular septal defect associated with heart failure and pulmonary hypertension (group 1); and six infants with tetralogy of Fallot, without heart failure (group 2). Interventions: Blood samples obtained from indwelling catheters or bypass circuit outlets. Measurements and Main Results: Nitrite and nitrate blood concentrations (as a marker for nitric oxide synthesis) and the molar ratio of cyclic guanosine 3',5'-monophosphate (cGMP) to ANP (as a marker for ANP biological activity) were determined before, during, and up to 24 hrs after cardiopulmonary bypass (CPB). In group 1 patients, these biological parameters were related to postoperative pulmonary arterial pressure. Preoperative nitrite and nitrate concentrations were higher in group 1 patients than in group 2 patients (p < .02), and this difference persisted during CPB. Nitrite and nitrate concentrations 24 hrs postoperatively were lower than preoperative values in group 1 patients (p < .05) end were unchanged in group 2 patients. An inverse correlation was observed postoperatively between nitrite and nitrate concentrations and systolic pulmonary arterial pressure (r2 = 0.4, p < .05). Group 1 patients had a lower preoperative cGMP/ANP ratio than group 2 patients (p < .05), despite higher ANP levels (p < .005). The cGMP/ANP ratio decreased during CPB in both groups (p < .0001), and in group 2 patients, cGMP and ANP values remained below preoperative values ≤24 hrs postoperatively. A correlation was observed between ANP levels and systolic pulmonary arterial pressure 2 and 4 hrs postoperatively (r2 = .4, p < .05, respectively), but no correlation was observed between ANP biological activity and postoperative pulmonary arterial pressure. Conclusions: Infants with heart failure and pulmonary hypertension have increased nitric oxide synthesis and decreased ANP biological activity; both phenomena may be involved in the pathophysiology of this clinical condition. CPB has no detectable effect on nitric oxide production but does decrease ANP biological activity. In patients with preoperative heart failure and pulmonary hypertension, endogenous nitric oxide appears to play a role in the control of postoperative pulmonary vascular tone. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailThe 37 LRP/P40 polypeptide: a multifunctional pleiotropic molecule involved in tumorigenesis and metastasis - A review
Clausse, Nathalie; Jackers, Pascale ULg; Castronovo, Vincenzo ULg

in Belgian Journal of Zoology (1997), 127(issue 1), 3-11

Detailed reference viewed: 72 (51 ULg)
Full Text
Peer Reviewed
See detailBulk polymerization of lactides initiated by aluminium isopropoxide, 2a). Beneficial effect of Lewis bases and transfer agents
Degée, Philippe; Dubois, Philippe ULg; Jérôme, Robert ULg

in Macromolecular Chemistry and Physics (1997), 198(6), 1973-1984

A high increase in the bulk polymerization rate of L,L-lactide and racemic D,L-lactide is observed when aluminium isopropoxide is added with an equimolar amount of a Lewis base, such as triphenylphosphine ... [more ▼]

A high increase in the bulk polymerization rate of L,L-lactide and racemic D,L-lactide is observed when aluminium isopropoxide is added with an equimolar amount of a Lewis base, such as triphenylphosphine (P(Φ)3) and better 4-picoline (C6H7N). Polylactide of high molecular weight and narrow molecular weight distribution is accordingly prepared in a controlled manner when the Al(OiPr)3 ; C6H7N combination is used as an initiator over a large temperature range from 110 to 180°C. A secondary alcohol, such as 2-propa-nol, has proved to be an efficient transfer agent, which allows the amount of Al per kg of polylactide to be advantageously reduced. All the alcohol molecules participate in the ring-opening polymerization even at an [iPrOH]0/[Al] ratio as high as 103. [less ▲]

Detailed reference viewed: 36 (8 ULg)
Full Text
Peer Reviewed
See detailSynthesis of novel conjugated oligomers for second-order nonlinear optics: incorporation of a central spacer as a conjugation modulator
Zhang, Jian Xang; Dubois, Philippe ULg; Jérôme, Robert ULg

in Journal of the Chemical Society. Perkin Transactions (1997), 2(6), 1209-1216

A new series of second-order nonlinear optical chromophores has been synthesized that consists of a conjugated segment end-capped with an electron acceptor and an electron donor, respectively, and a ... [more ▼]

A new series of second-order nonlinear optical chromophores has been synthesized that consists of a conjugated segment end-capped with an electron acceptor and an electron donor, respectively, and a central spacer intended to modulate the electro-optical effect. Conjugated chains have been tailored with trans-vinylene-1,5-thienylene as the building unit and N,N-dimethylamino and nitro groups as the donor–acceptor pair. Four spacers have been incorporated into the central part of the conjugated oligomers, which range from saturated to totally unsaturated functions, i.e. from methylene to vinylene units. The general strategy relies upon two consecutive Wittig or Wittig–Horner reactions between the spacer precursor and an aromatic phosphonium or phosphonate bearing the strong electron donor and the acceptor, respectively. Two synthetic pathways have been studied. The first procedure is based on the use of a symmetric precursor for the spacer. However, a reaction byproduct is formed, which must be removed and decreases the reaction efficiency. The second approach requires an asymmetric precursor for the spacer, the synthesis of which is a multistep process. In order to evaluate the effect of the spacer, a completely conjugated oligomer has been prepared by the one-step coupling of two conjugated segments end-functionalized by the electron donor and the acceptor, respectively. [less ▲]

Detailed reference viewed: 33 (3 ULg)
Peer Reviewed
See detailMeasuring Central Action of Acute Antimigraine Drugs in Humans
Schoenen, Jean ULg

in Cephalalgia : An International Journal of Headache (1997), 17(Suppl 17), 12-515-6

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailBulk polymerization of lactides initiated by aluminium isopropoxide, 3a). Thermal stability and viscoelastic properties
Degée, Philippe; Dubois, Philippe ULg; Jérôme, Robert ULg

in Macromolecular Chemistry and Physics (1997), 198(6), 1985-1995

The thermal stability of poly(L-lactide) and L/D-lactide stereocopolymers polymerized in bulk with aluminium isopropoxide as initiator was investigated by thermogravimetric analysis, and time and ... [more ▼]

The thermal stability of poly(L-lactide) and L/D-lactide stereocopolymers polymerized in bulk with aluminium isopropoxide as initiator was investigated by thermogravimetric analysis, and time and température dependence of molecular weight and polydispersity. Compared to poly(L-lactide) prepared with stannous octoate as catalyst, the thermal stability of polylactides is remarkably enhanced when the ring-opening polymerization is initiated by aluminium alkoxide. Ultimate mechanical properties and viscoelasticity of these melt-stable thermoplastics have also been studied in relation to the polyester enan-tiomeric composition. [less ▲]

Detailed reference viewed: 62 (7 ULg)
Full Text
See detailComment je traite ... une onychomycose par thérapie pulsée
Pierard, Gérald ULg

in Revue Médicale de Liège (1997), 52(6), 379-81

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailEnantioselective Determination of Oxprenolol in Human Plasma Using Dialysis Coupled on-Line to Reversed-Phase Chiral Liquid Chromatography
Ceccato, Attilio ULg; Toussaint, B.; Chiap, Patrice ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1997), 15(9-10), 1365-74

A fully automated method for the determination of the enantiomers of oxprenolol in human plasma was developed, involving dialysis through a cellulose acetate membrane, clean-up and enrichment of the ... [more ▼]

A fully automated method for the determination of the enantiomers of oxprenolol in human plasma was developed, involving dialysis through a cellulose acetate membrane, clean-up and enrichment of the dialysate on a short precolumn and subsequent chiral liquid chromatographic (LC) analysis. All sample handling operations were executed automatically by a sample processor equipped with a robotic arm (ASTED system). The trace enrichment column (TEC) was packed with octadecylsilica. After conditioning of the TEC with the LC mobile phase and pH 3.0 acetate buffer. After the enrichment step, the analyte was transferred by the LC mobile phase to the analytical column by means of a switching valve. The influence of different parameters of the dialysis process on the recovery of oxprenolol was first investigated using achiral LC conditions. The volume as well as the aspirating and dispensing flow rates of the acceptor solution were the main parameters studied. Oxprenolol was separated on a C18 stationary phase used for the enantioseparation of oxprenolol was a Chiralcel OD-R column which contained cellulose tris (3,5-dimethylphenylcarbamate) coated on silica as chiral selector. The corresponding mobile phase consisted of a mixture of pH 6.0 phosphate buffer containing NaClO4 at 0.45 M concentration and acetonitrile (70:30 v/v). UV detection was performed at 273 nm. The method developed was validated. Recoveries for each enantiomer of oxprenolol were about 80%. The method was found to be linear in the 50-2500 ng ml-1 concentration range (r2 = 0.999 for both enantiomers) and good results with respect to intra- and inter-day reproducibility as well as accuracy were obtained. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailLe rapport au capital, noyau dur de la coopération de production
Defourny, Jacques ULg

in Economie et Humanisme (1997), (341), 28-33

Detailed reference viewed: 78 (9 ULg)
Peer Reviewed
See detailStability of Steel Columns in Case of Fire : Numerical Modelling
Talamona, didier; Franssen, Jean-Marc ULg; Schleich, Jean-Baptiste et al

in Journal of Structural Engineering (1997), 123(6), 713-720

This paper describes the numerical simulations that have been performed within the frame of an ECSC reserch project. This research project lead to the buckling curve that has been considered in Eurocode 3 ... [more ▼]

This paper describes the numerical simulations that have been performed within the frame of an ECSC reserch project. This research project lead to the buckling curve that has been considered in Eurocode 3 for the design of steel colmns subjected to fire. [less ▲]

Detailed reference viewed: 43 (4 ULg)
Peer Reviewed
See detailRevascularisation coronaire multiple par pontages exclusivement artériels (mammaires internes et artère radiale gauche)
RADERMECKER, Marc ULg; Grenade, Thierry ULg; Cao-Thian, S.K. et al

in Revue Médicale de Liège (1997), 52(6), 377-8

Detailed reference viewed: 19 (0 ULg)
Full Text
Peer Reviewed
See detailPlasma Estradiol Concentrations and Pharmacokinetics Following Transdermal Application of Menorest 50 or Systen (Evorel) 50
Reginster, Jean-Yves ULg; Albert, Adelin ULg; Deroisy, Rita ULg et al

in Maturitas (1997), 27(2), 179-86

OBJECTIVES: In order to compare the pharmacokinetics of two transdermal estrogen replacement therapy (ERT) systems designed to release 50 micrograms 17 beta-estradiol/day, two studies were performed in ... [more ▼]

OBJECTIVES: In order to compare the pharmacokinetics of two transdermal estrogen replacement therapy (ERT) systems designed to release 50 micrograms 17 beta-estradiol/day, two studies were performed in healthy postmenopausal volunteers. METHODS: Both studies had a cross-over design and incorporated a 1-week wash-out period between treatments. In the first study, Menorest 50 and Systen 50 (Evorel 50) were compared over four days of application in 30 women. In the second, 13 women wore each of the two systems for a total of 12 days each (three patches each for 4 days), and comparison was made during the third patch period (steady state, between days 8 and 12). Plasma 17 beta-estradiol levels were assayed using specific direct radioimmunoassays, and pharmacokinetic parameters were calculated by standard methods. All the samples of the first study were re-analysed using a different radioimmunoassay and the results of both assays were compared. RESULTS: In both studies, plasma 17 beta-estradiol levels rose at a comparable rate and reached similar peak levels with each of the two formulations. Levels then remained relatively constant throughout both evaluation periods with Menorest 50, but began to decline after 12 hours in the first study and after 30 h under steady state conditions in the second study with Systen 50. The difference between the two products was statistically significant in both studies. Analysis of pharmacokinetic parameters confirmed the greater bioavailability of Menorest 50. In addition, 17 beta-estradiol levels remained within the suggested therapeutic ranges for relief of acute symptoms and protection against osteoporosis for longer periods of time with Menorest 50 than with Systen 50. CONCLUSION: Since the acute efficacy, long-term protective effects, side effects and risks associated with ERT may depend on critical threshold plasma levels, much attention should be paid to the pharmacokinetic profiles of different formulations. The comparison of these two different radioimmunoassays demonstrates the comparability of their results. [less ▲]

Detailed reference viewed: 28 (1 ULg)
Peer Reviewed
See detailAcute Migraine Therapy: The Newer Drugs
Schoenen, Jean ULg

in Current Opinion in Neurology (1997), 10(3), 237-43

In 1996, our knowledge of acute antimigraine therapy expanded in three major areas. First, large surveys have confirmed the remarkable efficacy profile of sumatriptan in clinical practice. No satisfying ... [more ▼]

In 1996, our knowledge of acute antimigraine therapy expanded in three major areas. First, large surveys have confirmed the remarkable efficacy profile of sumatriptan in clinical practice. No satisfying clinical, pharmacokinetic or genetic explanations were found for its major shortcomings: nonresponders, headache recurrence and noncardiac chest symptoms. Second, the novel 5-HT1B/D agonists zolmitriptan (311C90), rizatriptan (MK-462), eletriptan (UK-116,044), avitriptan (BMS-180048) and alniditan (R091274) were all proved superior to placebo for attack treatment, but their advantages over sumatriptan are yet to be analysed in more detail. A higher lipophilicity explains (except for alniditan) their greater oral bioavailability and better central nervous system penetration. A central action now proved experimentally in animals and in humans for 5-HT1B/D agonists such as zolmitriptan may be advantageous for the antimigraine efficacy, but it could also increase sedation. Third, an endothelin (Ro470203, bosentan) and a neurokinin 1 (RPR100893) receptor antagonist were found to be ineffective in migraine. Both compounds are potent inhibitors of neurogenic plasma extravasation in rat dura mater, which might suggest that this pharmacological property does not necessarily predict efficacy in aborting migraine attacks. [less ▲]

Detailed reference viewed: 27 (2 ULg)
Full Text
Peer Reviewed
See detailSuccessful Management of Acute Aortic Dissection in a Heart Transplant Recipient
Defraigne, Jean-Olivier ULg; Demoulin, J. C.; Detry, Olivier ULg et al

in Acta Chirurgica Belgica (1997), 97(3), 141-4

A case of type III aortic dissection which occurred fourteen months after heart transplantation is presented. Medical therapy was instituted to achieve controlled hypotension. The evolution was favorable ... [more ▼]

A case of type III aortic dissection which occurred fourteen months after heart transplantation is presented. Medical therapy was instituted to achieve controlled hypotension. The evolution was favorable and the patient could be discharged after one month. Hypertension and increased ejection fraction after transplantation could have been predisposing factors via an increase of the shear stress in the aorta. [less ▲]

Detailed reference viewed: 26 (0 ULg)
See detailREVEREND MILTON BRUNSON 1930-1997
Sacré, Robert ULg

in JAZZ HOT (1997), (541), 37

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailLigated anionic block copolymerization of methyl methacrylate with n-butyl acrylate and n-nonyl acrylate as promoted by lithium 2-(2-methoxyethoxy) ethoxide/diphenylmethyllithium
Nugay, Nihan; Nugay, Turgut; Jérôme, Robert ULg et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1997), 35(8), 1543-1548

Poly(methyl methacrylate-b-n-butyl acrylate) (PMMA-b-Pn-BuA) and poly(methyl methacrylate-b-n-nonyl acrylate) (PMMA-b-Pn-NonA) diblock copolymers have been successfully synthesized by the sequential ... [more ▼]

Poly(methyl methacrylate-b-n-butyl acrylate) (PMMA-b-Pn-BuA) and poly(methyl methacrylate-b-n-nonyl acrylate) (PMMA-b-Pn-NonA) diblock copolymers have been successfully synthesized by the sequential anionic polymerization of methyl methacrylate (MMA) and the n-alkyl acrylate (n-BuA or n-NonA), in a 90/10 toluene/ tetrahydrofuran (THF) mixture at -78°C. When diphenylmethyllithium (DPMLi) ligated with lithium 2-(2-methoxyethoxy) ethoxide (LiOEEM) is used as the initiator, the polymerization of each block appears to be living. Molecular weight and composition of block copolymers can be predicted from the monomer over initator molar ratio and the molecular weight distribution is narrow. Size exclusion chromatography (SEC) supports that no homo-PMMA contaminates the final copolymer. Although the reverse polymerization sequence Pn-NonA-b-PMMA always results in some contamination by homo-Pn-NonA, it has no really significant effect on the final product characteristics. [less ▲]

Detailed reference viewed: 27 (2 ULg)
Full Text
Peer Reviewed
See detailComparison of Biotyping, Ribotyping, and Pulsed-Field Gel Electrophoresis for Investigation of a Common-Source Outbreak of Burkholderia Pickettii Bacteremia
Chetoui, H.; Melin, Pierrette ULg; Struelens, M. J. et al

in Journal of Clinical Microbiology (1997), 35(6), 1398-403

Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection. Vials of a commercially available, "sterile" saline for ... [more ▼]

Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection. Vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. No further cases were diagnosed once the use of this saline was discontinued. Twenty-six isolates, including 9 outbreak-related strains from case patients and contaminated saline as well as 17 control strains, were tested comparatively by biotyping, ribotyping with EcoRI and HindIII, and pulsed-field gel electrophoresis (PFGE) with SpeI. Macrorestriction analysis revealed nine PFGE groups and was more discriminating than ribotyping (seven ribotypes) and biotyping (two biovars). Among the outbreak-related isolates, one B. pickettii type was found by the three typing methods. Furthermore, PFGE was useful for subdividing ribotypes and for distinguishing isolates involved in the outbreak from all epidemiologically unrelated strains. [less ▲]

Detailed reference viewed: 41 (1 ULg)
See detailDescription et commentaire de deux approches méthodologiques de la vidéo-évaluation des stages pratiques en didactique des langues germaniques à l'ULg (I)
Simons, Germain ULg

in Education-Formation (1997), (246), 43-49

La première partie de cet article (voir commentaire ci-dessous) porte sur la présentation et l'analyse d'un atelier de vidéo-évaluation des stages d'enseignement que nous proposons aux étudiants de l ... [more ▼]

La première partie de cet article (voir commentaire ci-dessous) porte sur la présentation et l'analyse d'un atelier de vidéo-évaluation des stages d'enseignement que nous proposons aux étudiants de l'Agrégation de l'Enseignement Secondaire Supérieur (AESS) en Langues et littératures germaniques à l'ULg. L'approche "confidentielle" de la vidéo-évaluation est une forme d'AUTOscopie qui repose sur cinq grandes étapes : 1. l'étudiant-stagiaire (E-S) est filmé pendant une leçon qu'il donne dans le cadre de ses stages ; 2. l'E-S exprime son ressenti par rapport à la leçon donnée (auto-évaluation "à chaud") puis visionne la vidéo (évaluation "à froid") ; 3. l'E-S est invité à identifier les différences entre son évaluation "à chaud" et "à froid" ; 4. le formateur complète cette auto-évaluation par son analyse de la leçon filmée ; 5. l'E-S. identifie les axes prioritaires sur lesquels il travaillera dans les stages suivants et le formateur fournit quelques pistes pour faciliter ce transfert dans la pratique. Les principaux avantages et inconvénients de cette approche de l'autoscopie sont abordés dans la conclusion provisoire de cet article. [less ▲]

Detailed reference viewed: 30 (4 ULg)
Peer Reviewed
See detailMyocardial Viability. Stress Echocardiography Vs Nuclear Medicine
Pierard, Luc ULg; Lancellotti, Patrizio ULg; Benoit, T.

in European Heart Journal (1997), 18(Suppl D), 117-23

Myocardial dyssynergy does not necessarily indicate myocardial necrosis in patients with coronary artery disease. The differentiation between viable and non-viable tissue is of great clinical importance ... [more ▼]

Myocardial dyssynergy does not necessarily indicate myocardial necrosis in patients with coronary artery disease. The differentiation between viable and non-viable tissue is of great clinical importance in order to make the most appropriate clinical decision in the individual patient. Several techniques are used to assess myocardial viability. Nuclear medicine gives reliable information on regional perfusion, metabolism and cell membrane integrity, while echocardiography provides real time visualization of myocardial thickening in basal conditions and continuously during pharmacological interventions. The presence or absence of contractile reserve in akinetic regions can be evaluated by pharmacological stress echocardiography. This article presents the semiology of myocardial viability as characterized by these different methods and reviews their relative value in different clinical settings. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailAmende et emprisonnement pour infraction : qui est visé ?
Delnoy, Michel ULg

in Immobilier (1997), 11

Detailed reference viewed: 27 (0 ULg)
Full Text
See detailL'expertise pénale du fond (enfin) contradictoire
Masset, Adrien ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (1997), 20(1997), 792-796

La Cour constitutionnelle belge est à l'origine d'une évolution majeure en procédure pénale en ce qui concerne le caractère contradictoire des expertises ordonnées en matière pénale.

Detailed reference viewed: 111 (0 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 22. Copolymerization of ε-caprolactone and 1,4,8-trioxaspiro[4.6]-9-undecanone initiated by aluminum isopropoxide
Tian, Dong; Dubois, Philippe ULg; Jérôme, Robert ULg

in Macromolecules (1997), 30(9), 2575-2581

Novel biodegradable and biocompatible polyesters have been prepared by random copolymerization of ε-caprolactone (ε-CL) and 1,4,8-trioxaspiro[4.6]-9-undecanone (TOSUO) initiated by Al(OiPr)3. Copolyesters ... [more ▼]

Novel biodegradable and biocompatible polyesters have been prepared by random copolymerization of ε-caprolactone (ε-CL) and 1,4,8-trioxaspiro[4.6]-9-undecanone (TOSUO) initiated by Al(OiPr)3. Copolyesters of molecular weight (Mn) up to 104 and containing up to 90 mol % TOSUO repeating units have been prepared. The copolymer molecular weight is controlled by the molar ratio of the consumed comonomers and the initiator. The TOSUO constitutive units have been quantitatively deacetalized into ketones, which have also been efficiently reduced into hydroxyl groups without any apparent chain degradation. Therefore, poly(ε-caprolactone) (PCL) with well-defined content of ketone or hydroxyl pendent groups have been made available. The ε-CL and TOSUO comonomer units are randomly distributed within the copolymers, as assessed by the 13C-NMR sequence analysis. Indeed, the reactivity ratios (rC and rT) have been calculated from the average length of the ε-CL sequences (LC) and the TOSUO sequences (LT) and found to be 1.3 (rC) and 1.0 (rT), respectively. There is an interesting parallelism between the crystallinity and the average length of the ε-CL sequences. Copolyesters containing a molar fraction of TOSUO units smaller than 0.15 are semicrystalline, whereas the incorporation of larger amounts of TOSUO units results in amorphous copolyesters. Conversion of the ethylene acetal pendent groups into ketones and ultimately into hydroxyl pendent groups results in increasing glass transition temperature (Tg), melting temperature (Tm), and melting enthalpy (ΔHm). The thermal stability depends on the copolyester composition, since it decreases as the molar fraction of TOSUO units is increased. [less ▲]

Detailed reference viewed: 32 (3 ULg)
Peer Reviewed
See detailRadiological Assessment of the Effects of a Full Rolling Motion Shoe During Asymmetrical Bearing
Caudron, Isabelle; Miesen, Myriam; Grulke, Sigrid ULg et al

in Equine Veterinary Journal. Supplement (1997), (23), 20-2

The authors used a new radiological method to assess asymmetrical articular compression of the interphalangeal joints. This method was based on the measurements of 3 angles obtained on dorsopalmar ... [more ▼]

The authors used a new radiological method to assess asymmetrical articular compression of the interphalangeal joints. This method was based on the measurements of 3 angles obtained on dorsopalmar radiographs. Variations of these angles were studied during experimental asymmetrical bearing on unshod feet. It was concluded that 2 angles were interesting parameters to assess asymmetrical articular compression and to define the position of the phalanx in the horny box. Furthermore, variations of these angles induced by experimental asymmetrical bearing were compared without shoe, with a standard shoe and with an orthopaedic full rolling motion shoe. It was observed that the effects of the asymmetrical bearing were reduced when feet were shod with a full rolling motion shoe. [less ▲]

Detailed reference viewed: 51 (5 ULg)
Full Text
Peer Reviewed
See detailDetermination of Six Water-Soluble Vitamins in a Pharmaceutical Formulation by Capillary Electrophoresis
Fotsing, Lucas ULg; Fillet, Marianne ULg; Bechet, I. et al

in Journal of Pharmaceutical & Biomedical Analysis (1997), 15(8), 1113-23

A method was developed for the quantitative analysis of six water-soluble vitamins (thiamine, nicotinamide, riboflavine, pyridoxine, ascorbic acid and pantothenic acid) in a pharmaceutical formulation ... [more ▼]

A method was developed for the quantitative analysis of six water-soluble vitamins (thiamine, nicotinamide, riboflavine, pyridoxine, ascorbic acid and pantothenic acid) in a pharmaceutical formulation, using free solution capillary zone electrophoresis (CZE) in uncoated fused silica capillaries and UV detection. The influence of different parameters, such as the nature of the buffer anionic component and buffer concentration on the CZE separation of vitamins was investigated using four vitamins of the B group as model compounds. A good compromise between resolution, analysis time and analyte stability was obtained by use of a 50 mM borax buffer of pH 8.5. This CZE method was found to be very useful for the separation of more complex samples, a mixture of ten water-soluble vitamins being completely resolved in about 10 min. However, cyanocobalamine could not be separated from nicotinamide in this CZE system, the two compounds being in uncharged form at the pH used. These two compounds could easily be resolved by micellar electrokinetic chromatography (MEKC), the anionic surfactant dodecylsulfate being added to the running buffer at 25 mM concentration. In the pharmaceutical formulation, some excipients were found to be adsorbed to the capillary surface, giving rise to a progressive decrease of the electroosmotic flow and consequently to a simultaneous increase of analyte migration times. A capillary wash with sodium hydroxide had to be made between successive runs in order to minimize these effects. Good results with respect to linearity, precision and accuracy were obtained in the concentration range studied for the six vitamins, using nicotinic acid as internal standard. [less ▲]

Detailed reference viewed: 89 (1 ULg)
Peer Reviewed
See detailLe monitoring thérapeutique des antidépresseurs
Charlier, Corinne ULg; Ansseau, Marc ULg; Gougnard, Thierry et al

in Revue Médicale de Liège (1997), 52(5), 336-44

Detailed reference viewed: 45 (8 ULg)
Peer Reviewed
See detailInhibition of the Blink Reflex R2 Component after Supraorbital and Index Finger Stimulations Is Reduced in Cluster Headache: An Indication for Both Segmental and Suprasegmental Dysfunction?
Lozza, A.; Schoenen, Jean ULg; Delwaide, P. J.

in Pain (1997), 71(1), 81-8

Peripheral as well as central mechanisms are thought to play a role in cluster headache pathogenesis. We have studied recovery curves of the R2 component of the blink reflex after conditioning by ... [more ▼]

Peripheral as well as central mechanisms are thought to play a role in cluster headache pathogenesis. We have studied recovery curves of the R2 component of the blink reflex after conditioning by supraorbital or index finger stimuli in 10 episodic cluster headache (CH) patients during a cluster period and in 10 healthy controls. There was no significant change of R2 threshold, latency or area in CH patients. After paired supraorbital stimuli, R2 recovered more rapidly in patients on the symptomatic side. After index stimulations, R2 recovery was more rapid on both symptomatic and non-symptomatic sides in patients compared to controls. Naloxone (0.4 mg) i.v. in two subjects partially reversed the R2 suppression induced by index finger stimuli. The unilateral decrease of R2 inhibition after a segmental supraorbital stimulus most likely reflects sensitisation in the spinal trigeminal nucleus. Whether the latter is due to irritation of the ophthalmic nerve within the cavernous sinus region, which is thought to be pivotal in CH pathogenesis, remains to be proven. In addition, we propose that the bilateral deficit of R2 inhibition after an extrasegmental exteroceptive stimulus might reflect hypoactivity of reticular nuclei, possibly because of reduced central opioid activity. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailCyclosporin C Is the Main Antifungal Compound Produced by Acremonium Luzulae
Moussaif, M.; Jacques, Philippe ULg; Schaarwachter, P. et al

in Applied and Environmental Microbiology (1997), 63(5), 1739-43

A strain of Acremonium luzulae (Fuckel) W. Gams was selected in screening new microorganisms for biological control of fruit postharvest diseases, especially gray and blue mold diseases on apples and ... [more ▼]

A strain of Acremonium luzulae (Fuckel) W. Gams was selected in screening new microorganisms for biological control of fruit postharvest diseases, especially gray and blue mold diseases on apples and strawberries. This strain manifests a very strong activity against a large number of phytopathogenic fungi. In this work, the product responsible for this antifungal activity was isolated from modified Sabouraud dextrose broth cultures of A. luzulae. It was purified to homogeneity by reverse-phase column chromatography. On the basis of UV, infrared, and 1H and 13C nuclear magnetic resonance spectra, mass spectral analysis, and the amino acid composition of the acid hydrolysates, the antibiotic was determined to be cyclosporin C. Cyclosporin C showed a broad-spectrum activity against filamentous phytopathogenic fungi but no activity against bacteria or yeasts. Its antifungal activity is only fungistatic. In contrast to Tolypocladium inflatum, another cyclosporin-producing strain, A. luzulae, did not produce additional cyclosporins. This was confirmed by in vivo-directed biosynthesis. [less ▲]

Detailed reference viewed: 25 (1 ULg)
Full Text
Peer Reviewed
See detailSeminoma arising in corrected and uncorrected inguinal cryptorchidism: Treatment and prognosis in 66 patients
Li; COUCKE, Philippe ULg; Tu-Nan, Qian et al

in International Journal of Radiation, Oncology, Biology, Physics (1997), 38(2), 343-350

Image : The purpose of this study was to analyze prognosis and treatment results for seminoma arising in corrected and uncorrected inguinal cryptorchidism (SCIC and SUIC). Image : We reviewed 66 patients ... [more ▼]

Image : The purpose of this study was to analyze prognosis and treatment results for seminoma arising in corrected and uncorrected inguinal cryptorchidism (SCIC and SUIC). Image : We reviewed 66 patients with inguinal seminomas between June 1958 and December 1991 at the Cancer Hospital and Institute of Chinese Academy of Medical Sciences. Of these patients, 23 had prior orchiopexy and 43 presented with an inguinal form of cryptorchidism. At presentation, 17 of 66 (26%) patients had nodal metastases. This nodal involvement was 30% (7 of 23) for SCIC and 23% (10 of 43) for SUIC, respectively. These numbers are comparable with those in a series of patients treated for scrotal seminoma at our institution (26% vs. 20%). However, 3 of 23 (13%) patients who had prior orchiopexy presented with inguinal nodal metastasis as compared with 0 of 43 patients with SUIC or 4 of 237 patients with scrotal seminoma (p < 0.5). There were 49 stage I, 5 stage IIA, 8 stage IIB, 3 stage III, and 1 stage IV patients. All patients underwent radical orchiectomy and received further radiotherapy, chemotherapy, or both. Patients with stage I and stage II disease were treated primarily with radiotherapy, whereas patients with stage III and IV disease were treated with chemotherapy. Image : The overall and disease-free survival at 5 and 10 years was 94% and 92%, 89% and 87%, respectively. The overall 5- and 10-year survival by stage was 100% and 100% for stage I, and 77% and 68% for stage II, respectively (p < .05). There was no significant difference in survival between SUIC and SCIC (93% vs. 96% at 5 years). Four patients developed relapse. Two of these four patients experienced relapse at the inguinal area, due to a marginal miss. Three of four patients with relapse were successfully salvaged, and one died of disease. Image : Our results indicate that prognosis for inguinal seminoma is excellent and similar to that of scrotal seminoma. Postorchiectomy radiotherapy can be considered as the standard treatment for stage I and IIA inguinal seminoma. We recommend routinely including the para-aortic and ipsilateral pelvic nodes. [less ▲]

Detailed reference viewed: 33 (2 ULg)
Full Text
Peer Reviewed
See detailInvolvement of 5-Hydroxytryptamine and Bradykinin in the Hyperalgesia Induced in Rats by Collagenase from Clostridium Histolyticum
Damas, Jacques ULg; Liégeois, Jean-François ULg; Bourdon, V.

in Naunyn-Schmiedeberg's Archives of Pharmacology (1997), 355(5), 566-70

The involvement of bradykinin, 5-hydroxytryptamine, substance P and prostanoids in the hyperalgesia elicited by collagenase in rat paw was investigated. Collagenase (100 micrograms) induced a slight ... [more ▼]

The involvement of bradykinin, 5-hydroxytryptamine, substance P and prostanoids in the hyperalgesia elicited by collagenase in rat paw was investigated. Collagenase (100 micrograms) induced a slight hyperalgesia in kininogen deficient rats in comparison with the behavioural response obtained in normal rats. Lisinopril (10(-5) M), and angiotensin-converting enzyme inhibitor, increased the duration of the hyperalgesia elicited in normal rats. Ondansetron (0.5 to 5 mumol/kg), a 5-HT3 antagonist, suppressed the hyperalgesia as did methysergide (1.1 to 11 mumol/kg), a mixed 5-HT1 and 5-HT2 receptor antagonist. However, the hyperalgesia was not modified by RP 67580 (1.8 to 18 mumol/kg), a NK1 receptor antagonist, and was only slightly delayed by indomethacin (2 mg/kg), a cyclo-oxygenase inhibitor. The oedema-promoting effect of 5-HT (6 nmol) was inhibited by methysergide but not by ondansetron. The swelling induced by collagenase in rat paw was reduced by methysergide but not by ondansetron. We conclude that the behavioural response induced by collagenase depends on an interactions between bradykinin and 5-HT. Prostanoids play a minor role in the beginning of the reaction whereas substance P is not significantly involved in this hyperalgesia. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailLe regard fou : Esquirol. La stratégie des interprétations
Strivay, Lucienne ULg

in Voir (1997), 14

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailInfractions et sanctions d'urbanisme : trois questions d'actualité
Delnoy, Michel ULg

in Formation permanente CUP (1997), XVII

Detailed reference viewed: 22 (1 ULg)
Peer Reviewed
See detailHet Congres voor de Vrijheid van de Cultuur
Vanden Berghe, Kristine ULg

in Onze Alma Mater (1997), 51(2), 193-211

Detailed reference viewed: 21 (2 ULg)
Peer Reviewed
See detailComment j'explore ... un patient avec diabète de type 1 inaugural ou a risque de développer la maladie
Philips, J. C.; Letiexhe, M. R.; Paquot, Nicolas ULg et al

in Revue Médicale de Liège (1997), 52(5), 363-6

Detailed reference viewed: 8 (6 ULg)
Full Text
Peer Reviewed
See detailLe regard invisible. Esquisse pour une théorie du troisième élément
Havelange, Carl ULg

in VOIR (barré) (1997), 14

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailOeil en pénombre
Strivay, Lucienne ULg; Gossiaux, Pol-Pierre ULg

in Voir (1997), 14

Detailed reference viewed: 38 (3 ULg)
Full Text
See detailLe pied tombant (foot drop). Un problème de diagnostic différentiel
Dubuisson, Annie ULg; Martin, Didier ULg; Lenelle, Jacques ULg et al

in Revue Médicale de Liège (1997), 52(5), 355-62

Detailed reference viewed: 189 (7 ULg)
Full Text
See detailReconnaître sur la peau des signes de maltraitance infantile
Pierard, Gérald ULg

in Revue Médicale de Liège (1997), 52(5), 320-3

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailComment je traite ... Un sujet a risque d'endocardite infectieuse
Kulbertus, Henri ULg; Pierard, Luc ULg

in Revue Médicale de Liège (1997), 52(5), 316

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailExpression of Stromelysin-3 in the Human Placenta and Placental Bed
Maquoi, Erik ULg; Polette, M.; Nawrocki, B. et al

in Placenta (1997), 18(4), 277-85

Human placentation is mediated by fetal trophoblastic cells which penetrate into the decidualized uterine endometrium. Trophoblast invasion requires the precisely regulated secretion of specific ... [more ▼]

Human placentation is mediated by fetal trophoblastic cells which penetrate into the decidualized uterine endometrium. Trophoblast invasion requires the precisely regulated secretion of specific proteinases able to degrade the endometrial basement membranes and extracellular matrix. To document further the involvement of these proteinases during human placentation, we evaluated in vivo the expression of stromelysin-3, a member of the metalloproteinase family, during the first and third trimesters of pregnancy, by means of immunohistochemistry, in situ hybridization and Northern blot analysis. Human extravillous trophoblasts invading the maternal decidua produced stromelysin-3 during both, the first and third trimesters of pregnancy, but to a lesser extent during the latter. In floating villi, stromelysin-3 expression was restricted to the syncytiotrophoblasts that line intervillous vascular spaces. In conclusion, stromelysin-3 is expressed by differentiated, non-proliferative villous and extravillous trophoblastic cells in early and late placental beds and villi, and its pattern of expression evolves during pregnancy. Our observations suggest that stromelysin-3 could play a role in human placentation. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailKetamine increases the amplitude of the 40-Hz auditory steady-state response in humans
PLOURDE, Gilles; BARIBEAU, Jacinthe; BONHOMME, Vincent ULg

in British Journal of Anaesthesia (1997), 78(5), 524-529

The auditory middle latency response (AMLR) and the 40-Hz auditory steady-state response (40-Hz ASSR) are evoked potentials which possibly arise from the same generators in the primary auditory cortex ... [more ▼]

The auditory middle latency response (AMLR) and the 40-Hz auditory steady-state response (40-Hz ASSR) are evoked potentials which possibly arise from the same generators in the primary auditory cortex. Both responses are attenuated by most general anaesthetics. Ketamine, however, has been reported to have no effect on the AMLR. Our aim was to evaluate the effects of ketamine on the 40-Hz ASSR. Spectral analysis of the electroencephalogram (EEG) was also conducted to independently examine the effects of ketamine. Ketamine 1.5 mg kg-1 was given to 12 patients for induction of general anaesthesia. Recordings of the 40-Hz ASSR and EEG were obtained every minute from 3 min before administration of ketamine to 5 min after injection, when the study was terminated. Similar recordings were obtained in three control subjects under identical conditions except that no medication was administered. Consciousness, defined as responsiveness to verbal commands, was assessed before each recording. Ketamine caused an increase in the amplitude of the 40-Hz ASSR (P < 0.01). Using published AMLR data, we conducted a simulation experiment that suggested that the effect of ketamine on the AMLR can explain its effects on the amplitude of the 40-Hz ASSR. There was a pronounced increase in relative theta (3.9-7.9 Hz) EEG power and a decrease in relative alpha (8.0-12.8 Hz) power (P < 0.001). These changes were not observed in the control group. Ketamine produced unconsciousness until the end of the study in five patients and transient unconsciousness in five patients. Two patients did not lose consciousness after administration of ketamine. The 40-Hz ASSR and EEG revealed no consistent differences between conscious and unconscious patients. No relationship could be demonstrated between the increase in amplitude of the 40-Hz ASSR or of relative theta power (the hallmark of ketamine effect) and loss of responsiveness to commands. We conclude that ketamine, unlike other anaesthetics, increases the amplitude of the 40-Hz ASSR. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailAcute Changes in Serum Calcium and Parathyroid Hormone Circulating Levels Induced by the Oral Intake of Five Currently Available Calcium Salts in Healthy Male Volunteers
Deroisy, Rita ULg; Zartarian, M.; Meurmans, L. et al

in Clinical Rheumatology (1997), 16(3), 249-53

Several calcium supplements are currently available and many of them are marketed without proper comparison of the bioavailability of the actual preparations. The aim of the present trial was to evaluate ... [more ▼]

Several calcium supplements are currently available and many of them are marketed without proper comparison of the bioavailability of the actual preparations. The aim of the present trial was to evaluate and compare the acute changes in serum calcium (Ca) and parathyroid hormone (PTH) levels following the oral administration of a vehicle and of five calcium salts currently prescribed in Western Europe. No significant changes in serum Ca or PTH levels were observed after administration of the vehicle. All calcium salts induced significant increases in serum Ca and decreases in serum PTH compared to baseline values. Comparison of the six response curves revealed a significantly greater increase in serum Ca and a greater decrease in serum PTH after each of the calcium salts than observed after the vehicle. However, no statistically significant differences were observed between the different calcium salts for serum Ca increments. The decrease in serum PTH observed after administration of an ossein-hydroxyapatite complex was significantly less important than after the four other calcium salts, even if statistically different than after vehicle. When assessing the area under the curve (AUC) of PTH values, we observed that calcium carbonate and citrate induce a significantly greater decrease in serum PTH than the other calcium salts which are, however, statistically more active than the vehicle. Serum PTH is decreased under the lower limit of the normal range (10 pg/ml), between t60 and t120 for calcium carbonate and citrate and between t60 and t90 for calcium gluconolactate while the mean PTH values remain within the normal range throughout the study with calcium pidolate, the ossein-hydroxyapatite complex and the vehicle. In conclusion, all calcium preparations significantly increase serum calcium and decrease serum parathormone, compared to what is observed after oral intake of a vehicle. However, significant differences in suppression of parathormone are observed between the different calcium preparations and might be of importance for their clinical use. [less ▲]

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailPolymerization-filled composites prepared with highly active filler-supported Al/Ti/Mg catalysts. I. Synthesis of homogeneous polyethylene-based composites
Hindryckx, François; Dubois, Philippe; Jérôme, Robert ULg et al

in Journal of Applied Polymer Science (1997), 64(3), 423-438

When it is attached onto the surface of an inorganic filler (kaolin, barite), the (BuO)4Ti/BuMgOct/EtAlCl2/Et3Al system is a highly efficient and very promising catalyst for the production of high ... [more ▼]

When it is attached onto the surface of an inorganic filler (kaolin, barite), the (BuO)4Ti/BuMgOct/EtAlCl2/Et3Al system is a highly efficient and very promising catalyst for the production of high-performance polyethylene-based composites. The surface concentration of the hydroxyl groups, the Al/Ti/Mg ratio, and the purity of the reagents have a major effect on the catalyst efficiency and molecular weight, crystallinity, and physico-mechanical properties of the final composite. Under ca. 4 bar partial pressure of ethylene in heptane at 60°C, the optimum Al/Ti/Mg catalyst composition is 120/0.75/10, the efficiency of which is as high as 300 kgPE/(gTi × h) in the presence of kaolin particles (Satintone W/W). The effect of transfer agents, such as hydrogen and 1-octene, and purity of the -olefin has been investigated. Actually, the catalyst efficiency is increased in the presence of both hydrogen and octene. Scanning Electron Microscopy (SEM) has shown that the ethylene was polymerized from the filler surface. Special attention has also been paid to the effect of size, density, and surface acidity of the filler particles. The surface of basic filler has to be treated in order to become an efficient catalyst carrier. [less ▲]

Detailed reference viewed: 11 (2 ULg)
Full Text
Peer Reviewed
See detailPolymerization-filled composites prepared with highly active filler-supported Al/Ti/Mg catalysts. II. Properties of homogeneous polyethylene-based
Hindryckx, François; Dubois, Philippe; Jérôme, Robert ULg et al

in Journal of Applied Polymer Science (1997), 64(3), 439-454

Polyethylene-based composites have been prepared by ethylene polymerization with a Al/Ti/Mg catalyst previously deposited on the surface of kaolin (Satintone W/W and Satintone 5) or barite.(1) Molecular ... [more ▼]

Polyethylene-based composites have been prepared by ethylene polymerization with a Al/Ti/Mg catalyst previously deposited on the surface of kaolin (Satintone W/W and Satintone 5) or barite.(1) Molecular weight (M(w)) of the polyethylene matrix has been estimated from melt viscosity under steady shear flow and compared to the value measured by size exclusion chromatography. Tensile stress and strain, Young modulus, and impact energy have been measured in relation to the matrix molecular weight and the filler content. The effect of the main polymerization parameters, such as the Al/Ti/Mg composition, the addition of a transfer agent, e.g., an alpha-olefin or hydrogen, have also been investigated. These tensile and impact properties have been compared to composites of the same composition but prepared by melt blending of the filler and the preformed polyolefin. As a rule, the polymerization-filled composites have an improved mechanical strength compared to the analogues prepared by melt blending. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Peer Reviewed
See detailUse of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak Operon
Brans, Alain ULg; Loriaux, Axelle; Thamm, Iris ULg et al

in FEMS Microbiology Letters (1997), 149(2), 189-94

The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond ... [more ▼]

The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters. [less ▲]

Detailed reference viewed: 45 (8 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 23. Synthesis and characterization of biodegradable and biocompatible homopolymers and block copolymers based on 1,4,8-trioxal [4.6] spiro-9-undecanone
Tian, Dong; Dubois, Philippe ULg; Jérôme, Robert ULg

in Macromolecules (1997), 30(7), 1947-1954

Homopolymers of 1,4,8-trioxa[4.6]spiro-9-undecanone (TOSUO) and block copolymers of TOSUO and ε-caprolactone (ε-CL) have been synthesized with aluminum isopropoxide as an initiator in toluene at 25 °C ... [more ▼]

Homopolymers of 1,4,8-trioxa[4.6]spiro-9-undecanone (TOSUO) and block copolymers of TOSUO and ε-caprolactone (ε-CL) have been synthesized with aluminum isopropoxide as an initiator in toluene at 25 °C. The homopolymerization is first order with respect to both monomer and initiator, and the end-group analysis agrees with a coordination−insertion mechanism based on the acyl-oxygen cleavage of the TOSUO ring. Living poly(ε-caprolactone) (PCL) and poly(1,4,8-trioxa[4.6]spiro-9-undecanone) (PTOSUO) chains are very efficient macroinitiators for the polymerization of TOSUO and ε-CL, respectively, with formation of block copolymers of a narrow molecular weight distribution. Size-exclusion chromatography (SEC) and 13C-NMR confirm the blocky structure of the copolymers, in agreement with DSC, which shows two glass transitions characteristic of the amorphous phase of PCL and PTOSUO, respectively. The ethylene ketal pendent groups of the PTOSUO block have been successfully converted to ketones and hydroxyl pendent groups without scission of the polyester backbone. These new materials have potential for applications in medicine, surgery, and tissue engineering. [less ▲]

Detailed reference viewed: 187 (3 ULg)
Full Text
Peer Reviewed
See detailVariations of the AP star HD 208217.
Manfroid, Jean ULg; Mathys, G.

in Astronomy and Astrophysics (1997), 320

We present a new determination of the rotation period of the Ap star HD 208217 based on photometric and magnetic measurements. The variations of this star are remarkable by the strong anharmonicity of its ... [more ▼]

We present a new determination of the rotation period of the Ap star HD 208217 based on photometric and magnetic measurements. The variations of this star are remarkable by the strong anharmonicity of its lightcurves in all colours as well as by the double wave of its mean magnetic field modulus variations. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailOn the Intercostal Muscle Compensation for Diaphragmatic Paralysis in the Dog
Brichant, Jean-François ULg; De Troyer, A.

in Journal of Physiology (1997), 500((Pt 1)), 245-53

1. Paralysis of the diaphragm in the dog is known to cause a compensatory increase in activation of the inspiratory intercostal muscles (parasternal intercostals, external intercostals, and levator costae ... [more ▼]

1. Paralysis of the diaphragm in the dog is known to cause a compensatory increase in activation of the inspiratory intercostal muscles (parasternal intercostals, external intercostals, and levator costae). The present studies were designed to assess the mechanism(s) of that compensation. 2. Complete, selective diaphragmatic paralysis was induced by injecting local anaesthetic into small silicone cuffs placed around the phrenic nerve roots in the neck. 3. Paralysis produced a decrease in tidal volume and an increase in arterial P(CO2) (P(a,CO2)). The increased hypercapnic drive was a primary determinant of the increased inspiratory intercostal activity. 4. However, paralysis also produced an increased inspiratory cranial displacement of the ribs. When this increased rib displacement was reduced to that seen before paralysis, it appeared that the increase in external intercostal and levator costae inspiratory activity was commonly greater than anticipated on the basis of the increased P(a,CO2). 5. Diaphragmatic paralysis after bilateral vagotomy also elicited disproportionate increases in inspiratory intercostal activity, thus indicating that these increases are not caused by vagal afferent inputs. 6. These observations are consistent with the idea that the intercostal muscle compensation for diaphragmatic paralysis is, in part, due to the release of an inhibition originating from the contracting diaphragm. This inhibition might arise in the diaphragmatic tendon organs. [less ▲]

Detailed reference viewed: 47 (4 ULg)
See detailLes constellations (II).
Renson, Pierre ULg

in Ciel et Terre (1997), 113

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailPulmonary Function Values in Friesian and Double-Muscled Calves During Acute Poikilocapnic Hypoxia
Rollin, Frédéric ULg; Amory, Hélène ULg; Desmecht, Daniel ULg et al

in Zentralblatt für Veterinarmedizin. Reihe A (1997), 44(2), 79-90

The effects of an acute non-isocapnic hypoxia on ventilation and pulmonary mechanics were investigated in 6 healthy unsedated Holstein-Friesian (HF) calves and in 7 double-muscled calves of the Belgian ... [more ▼]

The effects of an acute non-isocapnic hypoxia on ventilation and pulmonary mechanics were investigated in 6 healthy unsedated Holstein-Friesian (HF) calves and in 7 double-muscled calves of the Belgian White and Blue (BWB) breed known to develop more severe hypoxemia during exercise and respiratory diseases. Data were collected at 1 min intervals throughout the following protocol: breathing air (FIO2 = 21%), then breathing hypoxic gas mixture (FIO2 = 10%) for 5 min, and, finally, breathing air again for 5 min of recovery. Arterial blood was sampled at rest and at the 4th min of hypoxia for blood gas analysis. In HF and BWB calves, hypoxia induced a significant increase in tidal and minute volume, but did not change respiratory rate, dynamic lung compliance and total pulmonary resistance. However, with regard to their ventilation during normoxia at rest, BWB calves showed a comparatively greater rise in ventilation than HF calves during the first 4 min of hypoxia. On the contrary, by the 5th minute of hypoxia, ventilation of BWB calves declined to the normoxic level while HF calves largely sustained hyperventilation. This hypoxic ventilatory depression in BWB calves could be explained by the proportionately greater initial increase in ventilation. It was concluded that BWB calves supported this hypoxic challenge less easily than HF calves. [less ▲]

Detailed reference viewed: 45 (4 ULg)
Full Text
Peer Reviewed
See detailAnterior cingulate and motor network metabolic impairment in progressive supranuclear palsy
Salmon, Eric ULg; Van der Linden, Martial ULg; Franck, Georges ULg

in Neuroimage (1997), 5(3), 173-178

Progressive supranuclear palsy is the prototype of subcortical dementia. Using positron emission tomography and statistical parametric mapping, we compared the glucose metabolic pattern obtained in this ... [more ▼]

Progressive supranuclear palsy is the prototype of subcortical dementia. Using positron emission tomography and statistical parametric mapping, we compared the glucose metabolic pattern obtained in this subcortical dementia to that observed in elderly healthy controls and in Alzheimer's disease, the prototype of cortical dementia. Progressive supranuclear palsy was characterized by a relative decrease of metabolism in anterior cingulate, adjacent supplementary motor area, precentral cortex, middle prefrontal cortex, midbrain tegmentum, globus pallidus, and ventrolateral and dorsomedial nuclei of thalamus. The data in progressive supranuclear palsy highlight predominant metabolic impairment in brain structures engaged in response selection, in attention for action, and in motor networks. [less ▲]

Detailed reference viewed: 22 (0 ULg)
Peer Reviewed
See detailProblèmes diagnostiques, thérapeutiques et socio-économiques en oncologie gériatrique
Jerusalem, Guy ULg; Fillet, Georges ULg

in Revue Médicale de Liège (1997), 52(4), 255-7

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailA Positron Emission Tomography Study of Voluntarily and Electrically Contracted Human Quadriceps
Vanderthommen, Marc ULg; Depresseux, J. C.; Bauvir, Philippe ULg et al

in Muscle & Nerve (1997), 20(4), 505-7

Detailed reference viewed: 30 (4 ULg)
Full Text
Peer Reviewed
See detailSteroid Control and Sexual Differentiation of Brain Aromatase
Balthazart, Jacques ULg

in Journal of Steroid Biochemistry & Molecular Biology (1997), 61(3-6), 323-39

Brain aromatase (ARO) activity in the quail is markedly enhanced by testosterone (T). This effect only becomes detectable after several hours and reaches its maximum within a few days, which suggests ... [more ▼]

Brain aromatase (ARO) activity in the quail is markedly enhanced by testosterone (T). This effect only becomes detectable after several hours and reaches its maximum within a few days, which suggests enzymatic induction at the genomic level. This idea is reinforced by the fact that T also increases the ARO protein, as observed by immunocytochemistry (ICC) and the ARO mRNA, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR). These changes can be mimicked by the administration of estrogens and therefore presumably require T aromatization. In our first test, injection of the non-steroidal ARO inhibitor, R76713 (racemic vorozole), unexpectedly revealed an increase in ARO immunoreactivity in the preoptic area (POA) of treated birds. This property of R76713 was shared by another non-steroidal inhibitor, fadrozole, but not by two steroidal inhibitors, androstatrienedione (ATD) and 4-hydroxy-androstenedione (OHA). These last two compounds markedly decreased the concentration of brain ARO as estimated by ICC. In parallel, ATD and OHA decreased ARO mRNA concentration measured by RT-PCR but vorozole and fadrozole had no effect on these concentrations in the POA, and only caused them to decrease slightly in the posterior hypothalamus. Together, these data indicate that the removal of estrogens caused by steroidal inhibitors decreases the synthesis of ARO, presumably at the transcriptional level. Additional regulatory mechanisms apparently take place after the injection of non-steroidal inhibitors and probably include increased half-life of the protein. The induction of ARO activity by steroids appears to be greater in males than in females, but this difference has been difficult to localize and confirm by assay methods. We therefore analysed by ICC the tridimensional distribution of ARO-ir neurons in the POA of males and females that were sexually mature or gonadectomized and treated with T-filled or control empty implants. Localized sex differences and effects of T were detected in this way. In particular, males had more ARO-ir cells than females in the lateral POA but a difference in the opposite direction was evident in the medial part of this area. These sex differences are largely activational (i.e. caused by the higher T levels in males) but they may also reflect organizational effects of neonatal steroids. Castration decreased ARO-ir cell numbers in the lateral POA, but increased it in the periventricular region. This anatomically specialized control by T may be mediated by three potential mechanisms that are discussed and comparatively evaluated: a migration of ARO neurons towards the ventricle after castration; a differential colocalization of ARO with estrogen receptors or a differential modulation of ARO neurons by catecholaminergic inputs. [less ▲]

Detailed reference viewed: 14 (3 ULg)
Full Text
Peer Reviewed
See detailMild Endotoxaemia and the Inflammatory Response Induced by a Marathon Race
Camus, G.; Poortmans, J.; Nys, Monique ULg et al

in Clinical Science (1997), 92(4), 415-22

1. To address the question of whether endotoxaemia could be involved in the inflammatory response induced by long-term strenuous exercise, 18 male marathon runners [mean age 41 +/- 2 (SEM) years] were ... [more ▼]

1. To address the question of whether endotoxaemia could be involved in the inflammatory response induced by long-term strenuous exercise, 18 male marathon runners [mean age 41 +/- 2 (SEM) years] were studied. Their performance in the marathon ranged from 2 h 46 min to 4 h 42 min. 2. Four venous blood samples were drawn: at rest, just before the race (baseline); within 15 min following the completion of the marathon; after 1 h of recovery; and the morning after the race. 3. The following humoral markers of the inflammatory response to exercise were measured: polymorphonuclear myeloperoxidase (MPO), anaphylatoxin C5a (C5a), tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Plasma endotoxin was measured by a sensitive and rapid chromogenic Limulus assay. All inflammatory markers were significantly increased (P < 0.001) after the race, reaching in most cases peak values in the first blood sample drawn following the completion of the marathon [MPO, 298 +/- 19 ng/ml (SEM); C5a, 1.45 +/- 0.32 ng/ml; TNF-alpha, 20 +/- 3 pg/ml; IL-6, 88 +/- 13 pg/ml] when compared with baseline [MPO, 146 +/- 16 ng/ml (SEM); C5a, 0.27 +/- 0.2 ng/ml; TNF-alpha, 12 +/- 1.5 pg/ml: IL-6, 1.0 +/- 0.5 pg/ml]. Traces of plasma endotoxin (ranging from 5 to 13 pg/ml, with one exceptionally high value of 72 pg/ml measured in one runner) were detected in seven subjects within the first hour of recovery. An ELISA method was used to determine the endogenous IgG antibodies toward a range of Gram-negative bacterial lipopolysaccharides (LPSs) of different sizes and structures. A transient decrease in certain anti-LPS activities, mainly against rough LPS, occurred in most cases in the first blood sample drawn after the race. There was no correlation between the magnitude of the inflammatory response to exercise, as assessed by the increase in blood levels of humoral markers of inflammation, and the changes in circulating endotoxin levels of anti-LPS IgG activity following the race. 4. From these results, we conclude that the mild, transient endotoxaemia detected in some of our subjects does not play a major role in the observed inflammatory response to a marathon competition. [less ▲]

Detailed reference viewed: 33 (1 ULg)
Peer Reviewed
See detailVieillissement de la population: données démographiques pour la région Liégeoise
Paul, I.; Gosset, Christiane ULg

in Revue Médicale de Liège (1997), 52(4), 190-2

Detailed reference viewed: 21 (2 ULg)
Full Text
Peer Reviewed
See detailStudy of the Influence of Both Cyclodextrins and L-Lysine on the Aqueous Solubility of Nimesulide; Isolation and Characterization of Nimesulide-L-Lysine-Cyclodextrin Complexes
Piel, Géraldine ULg; Pirotte, Bernard ULg; Delneuville, Isabelle et al

in Journal of Pharmaceutical Sciences (1997), 86(4), 475-80

Nimesulide is a nonsteroidal antiinflammatory drug that exhibits a very poor water solubility (0.01 mg.mL-1). A nimesulide-beta-cyclodextrin complex prepared according to patent application WO 94/ 02177 ... [more ▼]

Nimesulide is a nonsteroidal antiinflammatory drug that exhibits a very poor water solubility (0.01 mg.mL-1). A nimesulide-beta-cyclodextrin complex prepared according to patent application WO 94/ 02177 has an aqueous solubility of approximately 16 mg.mL-1 of nimesulide. A nimesulide-L-lysine salt has also been prepared and increases the aqueous solubility of nimesulide to approximately 5.0-7.5 mg.mL-1. The purpose of the present study was to investigate the interaction of both cyclodextrins and L-lysine on the aqueous solubility of nimesulide. Nimesulide-L-lysine-beta- or gamma-cyclodextrin complexes were prepared by spray-drying. The inclusion of the nimesulide-L-lysine salt into the cyclodextrin cavity was confirmed by differential scanning calorimetry and proton nuclear magnetic resonance spectroscopy. These complexes offered remarkable aqueous solubility. The incorporation of nimesulide in a nimesulide-L-lysine-beta-cyclodextrin complex increased its water solubility by a factor of 10 at pH 1.5 (0.050 mg.mL-1 for the complex versus 0.005 mg.mL-1 for nimesulide), 160 at pH 6.8 (2.373 mg.mL-1 for the complex versus 0.015 mg.mL-1 for nimesulide), and 3600 in purified water (36.400 mg.mL-1 for the complex versus 0.01 mg.mL-1 for nimesulide). [less ▲]

Detailed reference viewed: 28 (6 ULg)
Full Text
Peer Reviewed
See detailChirurgie carotidienne au-delà de 75 ans
Van Damme, Hendrik ULg; Albert, Adelin ULg; Limet, Raymond

in Revue Médicale de Liège (1997), 52(4), 237-44

Detailed reference viewed: 1 (0 ULg)
Peer Reviewed
See detailLa maladie d'Alzheimer
Salmon, Eric ULg; Georges, Michel ULg; Collette, Fabienne ULg

in Revue Médicale de Liège (1997), 52(4), 258-261

La maladie d'Alzheimer touche plus de 5% de notre population adulte, et sa prévalence augmente avec l'äge. La symptomatologie clinique permet un diagnostic de haute probabilité, mais les lésions ... [more ▼]

La maladie d'Alzheimer touche plus de 5% de notre population adulte, et sa prévalence augmente avec l'äge. La symptomatologie clinique permet un diagnostic de haute probabilité, mais les lésions histologiques de dégénérescence neurofibrillaire et les plaques séniles restent les seuls critères diagnostiques de certitude. Les recherches en biologie moléculaire visent à comprendre le mécanisme pathogène afin de pouvoir l'interrompre. En attendant, notre prise en charge est symptomatique, et le but est d'optimiser la qualité de vie du patient et de ses proches. [less ▲]

Detailed reference viewed: 39 (12 ULg)
Peer Reviewed
See detailLes manifestations osteo-articulaires du sujet âgé
Andre, Béatrice ULg; Ribbens, Clio ULg; Kaye, O. et al

in Revue Médicale de Liège (1997), 52(4), 270-277

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailGlycoprotein B of Bovine Herpesvirus 4 Is a Major Component of the Virion, Unlike That of Two Other Gammaherpesviruses, Epstein-Barr Virus and Murine Gammaherpesvirus 68
Lomonte, P.; Filée, Patrice ULg; Lyaku, J. R. et al

in Journal of Virology (1997), 71(4), 3332-5

This study reports that in bovine herpesvirus 4, glycoprotein B (gB) is a heterodimer and a major component of the virion, unlike gBs of Epstein-Barr virus (gp110) and murine gammaherpesvirus 68, two ... [more ▼]

This study reports that in bovine herpesvirus 4, glycoprotein B (gB) is a heterodimer and a major component of the virion, unlike gBs of Epstein-Barr virus (gp110) and murine gammaherpesvirus 68, two other gammaherpesviruses. These are new characteristics with regard to the general features of gB in the Gammaherpesvirinae subfamily. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailCentral pontine myelinolysis associated with acquired folate depletion
RAMAEKERS, Vincent ULg; Reul, J.; Kusenbach, G. et al

in Neuropediatrics (1997), 28(2), 126-30

After long-standing malnutrition a 15-month-old boy with signs of kwashiorkor was admitted in a moribund state with serious hyponatraemic dehydration, hypothermia, somnolence, and signs of a pontine ... [more ▼]

After long-standing malnutrition a 15-month-old boy with signs of kwashiorkor was admitted in a moribund state with serious hyponatraemic dehydration, hypothermia, somnolence, and signs of a pontine disconnection syndrome. Folic acid levels were below the detection level in the presence of normal cobalamin levels. MRI of the brain showed global volume loss and signal abnormalities on the T2-weighted images suggestive for central pontine myelinolysis (CPM). Brainstem acoustic evoked responses have remained normal. The serious metabolic and nutritional derangements required substitution of folic acid, vitamins and trace elements as well as slow correction of hyponatraemic dehydration with return of the sodium level over a period of four days. This therapeutic regimen resulted in complete neurological recovery. Follow-up MRI documented normalisation of the initial pathologic findings. The hypothesis was put forward linking the pathogenesis of CPM with the combination of folate depletion and superimposed hyponatraemic dehydration. The previously acquired folate depletion could affect normal appositional function of myelin basic protein molecules due to insufficient methylation of arginine in position 107. The subsequent development of intramyelinic edema and CPM will then be triggered by the superimposed hyponatraemic dehydration. The verification of this hypothesis requires further investigations. [less ▲]

Detailed reference viewed: 12 (0 ULg)