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See detailTransgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery.
Plumier, Jean-Christophe ULg; Ross, B.; Currie, R. W. et al

in Journal of Clinical Investigation (1995), 95(4), 1854-60

Heat shock treatment induces expression of several heat shock proteins and subsequent post-ischemic myocardial protection. Correlations exist between the degree of stress used to induce the heat shock ... [more ▼]

Heat shock treatment induces expression of several heat shock proteins and subsequent post-ischemic myocardial protection. Correlations exist between the degree of stress used to induce the heat shock proteins, the amount of the inducible heat shock protein 70 (HSP70) and the level of myocardial protection. The inducible HSP70 has also been shown to be protective in transfected myogenic cells. Here we examined the role of human inducible HSP70 in transgenic mouse hearts. Overexpression of the human HSP70 does not appear to affect normal protein synthesis or the stress response in transgenic mice compared with nontransgenic mice. After 30 min of ischemia, upon reperfusion, transgenic hearts versus nontransgenic hearts showed significantly improved recovery of contractile force (0.35 +/- 0.08 versus 0.16 +/- 0.05 g, respectively, P < 0.05), rate of contraction, and rate of relaxation. Creatine kinase, an indicator of cellular injury, was released at a high level (67.7 +/- 23.0 U/ml) upon reperfusion from nontransgenic hearts, but not transgenic hearts (1.6 +/- 0.8 U/ml). We conclude that high level constitutive expression of the human inducible HSP70 plays a direct role in the protection of the myocardium from ischemia and reperfusion injury. [less ▲]

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See detailFunctional analysis of the Antirrhinum floral homeotic DEFICIENS gene in vivo and in vitro by using a temperature-sensitive mutant.
Zachgo, S.; Silva, E de A; Motte, Patrick ULg et al

in Development (1995), 121(9), 2861-75

Flowers of the temperature-sensitive DEFICIENS (DEF) mutant, def-101, display sepaloid petals and carpelloid stamens when grown at 26 degrees C, the non-permissive temperature. In contrast, when ... [more ▼]

Flowers of the temperature-sensitive DEFICIENS (DEF) mutant, def-101, display sepaloid petals and carpelloid stamens when grown at 26 degrees C, the non-permissive temperature. In contrast, when cultivated under permissive conditions at 15 degrees C, the morphology of def-101 flowers resembles that of the wild type. Temperature shift experiments during early and late phases of flower development revealed that second and third whorl organ development is differentially sensitive to changes in DEF expression. In addition, early DEF expression seems to control the spatially correct initiation of fourth whorl organ development. Reduction of the def-101 gene dosage differentially affects organogenesis in adjacent whorls: at the lower temperature development of petals in the second whorl and initiation of carpels in the centre of the flower is not affected while third whorl organogenesis follows the mutant (carpelloid) pattern. The possible contribution of accessory factors to organ-specific DEF functions is discussed. In situ analyses of mRNA and protein expression patterns during def-101 flower development at 15 degrees C and at 26 degrees C support previously proposed combinatorial regulatory interactions between the MADS-box proteins DEF and GLOBOSA (GLO), and provide evidence that the autoregulatory control of DEF and GLO expression by the DEF/GLO heterodimer starts after initiation of all organ primordia. Immunolocalisation revealed that both proteins are located in the nucleus. Interestingly, higher growth temperature affects the stability of both the DEF-101 and GLO proteins in vivo. In vitro DNA binding studies suggest that the temperature sensitivity of the def-101 mutant is due to an altered heterodimerisation/DNA-binding capability of the DEF-101 protein, conditioned by the deletion of one amino acid within the K-box, a protein region thought to be involved in protein-protein interaction. In addition, we introduce a mutant allele of GLO, glo-confusa, where insertion of one amino acid impairs the hydrophobic carboxy-terminal region of the MADS-box, but which confers no strong phenotypic changes to the flower. The strong mutant phenotype of flowers of def-101/glo-conf double mutants when grown in the cold represents genetic evidence for heterodimerisation between DEF and GLO in vivo. The potential to dissect structural and functional domains of MADS-box transcription factors is discussed. [less ▲]

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See detailNetherton’s syndrome in two sisters. A ten year experience of therapy with retinoids
Groves, S.; Dezfoulian, Bita ULg; Bonardeaux, Ch et al

in Journal of the European Academy of Dermatology & Venereology (1995)

Netherton’s syndrome is a recessive autosomal disease associating ichthyosiform dermatosis, hair dysplasia and systemic involvement. (Netherton, 1958, Arch. Dermatol., Vol. 78,483-487). We report the ... [more ▼]

Netherton’s syndrome is a recessive autosomal disease associating ichthyosiform dermatosis, hair dysplasia and systemic involvement. (Netherton, 1958, Arch. Dermatol., Vol. 78,483-487). We report the observation of two sisters who present the complete form of Netherton’s syndrome. Both patients were treated with retinoids for more than ten years. [less ▲]

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See detailRecommendations for the registration of new chemical entities used in the prevention and treatment of osteoporosis
Reginster, Jean-Yves ULg; Compston, JE; Jones, EA et al

in Calcified Tissue International (1995), 57

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See detailL'hyperthyroïdie sévère associée à la prise d'amodarone: une urgence chirurgicale
Meurisse, Michel ULg; Hamoir, Etienne ULg; Joris, Jean ULg et al

in Revue Française d'Endocrinologie Clinique, Nutrition, et Métabolisme (La) (1995), 36(1), 35-45

L'évolution de certaines thyréotoxicoses associées à la prise d'amiodarone peut être particulièrement grave et rapide, menaçant le pronostic vital. De déclenchement brutal, sans relation avec la durée du ... [more ▼]

L'évolution de certaines thyréotoxicoses associées à la prise d'amiodarone peut être particulièrement grave et rapide, menaçant le pronostic vital. De déclenchement brutal, sans relation avec la durée du traitement par amiodarone, elles peuvent survenir aussi bien chez des sujets porteurs de thyroïdopathies méconnues ou négligées au moment de l'induction du traitement, que chez des patients apparemment indemnes de toute pathologie thyroïdienne pré-existante. Le traitement médical conservateur est le plus souvent inefficace. Dans ces cas, il convient de poser sans hésitation, l'indication d'une thyroïdectomie la plus radicale possible. Ce rapport traite de l'évolution post-opératoire spectaculaire et favorable de 13 cas consécutifs de thyréotoxicose sévère associée à la prise d'amiodarone. La validité de l'approche chirurgicale repose sur la correction rapide et définitive de l'état toxique après exérèse glandulaire. La morbidité quasi nulle liée au geste chirurgical est la seule alternative thérapeutique antithyroïdienne existante qui permette de poursuivre un traitement par amiodarone lorsque celui-ci est indiqué pour contrôler une arytmie menaçante [less ▲]

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See detailFamilial trends of obesity through three generations: the Belgian-Luxembourg child study.
Guillaume, Michèle ULg; Lapidus, L.; Beckers, F. et al

in International Journal of Obesity (1995), 19 Suppl 3

Province de Luxembourg in Belgium is an area with clustering of obesity as well as other risk factors for cardiovascular diseases and non-insulin dependent diabetes mellitus. In this study, obesity was ... [more ▼]

Province de Luxembourg in Belgium is an area with clustering of obesity as well as other risk factors for cardiovascular diseases and non-insulin dependent diabetes mellitus. In this study, obesity was studied in a total of 1028 boys and girls in age-strata 6-8, 8-10, 10-12 years, selected at random from school classes. Participation rate was about 70%. Non-participants did not differ from participants in comparisons of school records of height and weight. Furthermore, information on birth weight, parents' height and weight was collected, as well as reported problems of obesity in grandparents. The results show a high prevalence of pronounced obesity in these children. BMIs were strongly correlated between the children and both parents. Furthermore, grandparents' obesity problems were related to the BMI of parents, and also to obesity indices in the children. Birth weights were related to current BMI of the children (in girls only for the youngest age-group), and to their mothers' BMI. It was concluded that obesity is prevalent in this area and can be traced through three generations, and seems to be discoverable already at birth. Energy intake is high and physical activity level is low in these children. However, statistical analyses suggest that familial factors exert a greater impact than environmental factors on the BMI of the children. These results, as well as the wide-spread, consistent familial clustering of obesity, traceable already at birth, suggest influence of strong genetic traits for obesity in this population. [less ▲]

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See detailActivation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro.
Sheridan, P. L.; Sheline, C. T.; Cannon, K. et al

in Genes & Development (1995), 9(17), 2090-104

Lymphoid enhancer-binding factor 1 (LEF-1) is a regulatory high mobility group (HMG) protein that activates the T cell receptor alpha (TCR alpha) enhancer in a context-restricted manner in T cells. In ... [more ▼]

Lymphoid enhancer-binding factor 1 (LEF-1) is a regulatory high mobility group (HMG) protein that activates the T cell receptor alpha (TCR alpha) enhancer in a context-restricted manner in T cells. In this paper we demonstrate that the distal region of the human immunodeficiency virus-1 (HIV-1) enhancer, which contains DNA-binding sites for LEF-1 and Ets-1, also provides a functional context for activation by LEF-1. First, we show that mutations in the LEF-1-binding site inhibit the activity of multimerized copies of the HIV-1 enhancer in Jurkat T cells, and that LEF-1/GAL4 can activate a GAL4-substituted HIV-1 enhancer 80- to 100-fold in vivo. Second, recombinant LEF-1 is shown to activate HIV-1 transcription on chromatin-assembled DNA in vitro. By using a nucleosome-assembly system derived from Drosophila embryos, we find that the packaging of DNA into chromatin in vitro strongly represses HIV-1 transcription and that repression can be counteracted efficiently by preincubation of the DNA with LEF-1 (or LEF-1 and Ets-1) supplemented with fractions containing the promoter-binding protein, Sp1. Addition of TFE-3, which binds to an E-box motif upstream of the LEF-1 and Ets-1 sites, further augments transcription in this system. Individually or collectively, none of the three enhancer-binding proteins (LEF-1, Ets-1, and TFE-3) could activate transcription in the absence of Sp1. A truncation mutant of LEF-1 (HMG-88), which contains the HMG box but lacks the trans-activation domain, did not activate transcription from nucleosomal DNA, indicating that bending of DNA by the HMG domain is not sufficient to activate transcription in vitro. We conclude that transcription activation by LEF-1 in vitro is a chromatin-dependent process that requires a functional trans-activation domain in addition to the HMG domain. [less ▲]

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See detailHet is nooit goed. Marginalia bij De pasvorm van de goede smaak van Marc Reynebeau
Spinoy, Erik ULg

in Yang : Tijdschrift voor Literatuur en Kommunikatie (1995), 31(3-4), 111-121

This article criticizes the illusions of normative literary criticism, which hopes to determine 'once and for all' the true and objective value of literature.

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See detailToxicité potentielle et utilisation pratique du méthotrexate dans la polyarthrite rhumatoïde
Kaye, O.; Ribbens, Clio ULg; Hauwaert, Christian ULg et al

in Médecine et Hygiène (1995)

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See detailCharacterization and partial amino acid sequencing of a 107-kDa procollagen I N-proteinase purified by affinity chromatography on immobilized type XIV collagen.
Colige, Alain ULg; Beschin, A.; Samyn, B. et al

in Journal of Biological Chemistry (1995), 270(28), 16724-30

Procollagen I N-proteinase (EC 3.4.24.14), the enzyme that specifically processes type I and type II procollagens to collagen, was isolated from extracts of fetal calf skin. After two chromatographic ... [more ▼]

Procollagen I N-proteinase (EC 3.4.24.14), the enzyme that specifically processes type I and type II procollagens to collagen, was isolated from extracts of fetal calf skin. After two chromatographic steps on concanavalin A-Sepharose and heparin-Sepharose, the semi-purified preparation was used to produce monoclonal antibodies. One reacting antibody was found to recognize not the enzyme itself but type XIV collagen on which the enzyme was bound. This binding, highly sensitive to ionic conditions (plH, salt concentrations) but not affected by non-ionic detergents, was used for affinity chromatography that strongly improved the purification procedure. The enzyme is extensively characterized: 1) it has a molecular mass of 107 kDa as determined by polyacrylamide gel electrophoresis in presence of SDS and of about 130 kDa when estimated by gel filtration on a Sephacryl-S300; 2) in standard assay (pH 7.5, 0.2 M NaCl, 35 degrees C), the activation energy for reaction with amino procollagen type I was 17,000 calories per mole. In the same conditions, Km and Vmax values were, respectively, 435 and 39 nM per hour but varied strongly with pH and salt concentration; 3) the enzyme cleaved the NH2-terminal propeptide of type I procollagen at the specific site, the Pro-Gln bond in the alpha 1 type I procollagen chain; 4) the enzyme contained a high proportion of Gly, Asx, and Glx residues but no Hyp or Hyl; 5) partial amino acid sequences obtained from internal peptides of the enzyme displayed no significant homology with known sequences. The association of procollagen I N-proteinase with a FACIT (fibril-associated collagens with interrupted triple helices) collagen as found here might be of physiological significance. [less ▲]

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See detailFactors regulating megakaryocyte progenitor commitment to polyploidization.
Paulus, Jean-Michel ULg; Grosdent, J C; Prenant, M et al

in Comptes Rendus de l'Académie des Sciences. Série III. Sciences de la Vie (1995), 318(3), 381-6

Recognizable megakaryocytes are polyploid cells generated by a clonogenic, diploid progenitor, termed CFU-MKC (colony forming unit, megakaryocyte). In order to quantify polyploidization, ploidy histograms ... [more ▼]

Recognizable megakaryocytes are polyploid cells generated by a clonogenic, diploid progenitor, termed CFU-MKC (colony forming unit, megakaryocyte). In order to quantify polyploidization, ploidy histograms of megakaryocytes determined by microphotometric or flow cytometric measurements of megakaryocyte DNA have generally been used. However these techniques provide no information on the rate of commitment of CFU-MKC to polyploidy. Using a technique of clonal analysis determining the distributions of the number of doublings (NbD) undergone by CFU-MKC before committing to polyploidization, the polyploidization probability of CFU-MKC could be derived. This probability was found to be a constant independent from CFU-MKC mitotic history, since NbD distributions are exponential functions characterized by a constant rate of decay per doubling. By studying the effects of growth factors on polyploidization probability, it was also shown that: (1) this parameter is negatively regulated by growth factors contained in poke-weed or WEHI conditioned media, as well as by erythropoietin; (2) commitment to polyploidization does not require prior CFU-MKC division; (3) bipotent erythroid-megakaryocyte progenitors have a lower polyploidization probability than CFU-MKC; (4) determination of polyploidization probability reflects the activity of growth factors with greater accuracy than megakaryocyte colony count. [less ▲]

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See detailPyoverdine-mediated uptake of iron in Pseudomonas putida BTP1 : involvement of a 100 KD IROMP.
Delfosse, Ph; Ongena, Marc ULg; Jacques, Ph et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1995), 103(B39),

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See detailGlucose inhibits human placental GH secretion, in vitro
Patel, N.; Alsat, E.; Igout, Ahmed ULg et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(5), 1743-6

Human placenta specifically expresses the GH-V gene leading to the production of placental Growth Hormone (PGH). During pregnancy, PGH levels increase progressively in maternal blood, but its regulation ... [more ▼]

Human placenta specifically expresses the GH-V gene leading to the production of placental Growth Hormone (PGH). During pregnancy, PGH levels increase progressively in maternal blood, but its regulation remains unknown. In this study the effect of glucose on PGH secretion by human term placenta was tested, in vitro, by means of two different experimental models: organ culture of villous tissue and primary culture of isolated cytotrophoblasts. PGH was assayed in the culture medium by an immunoradiometric assay using a specific PGH monoclonal antibody. The presence of glucose (25 mmol/L) in the culture medium significantly inhibited (p < 0.001) the secretion of PGH by either placental villous explants or by cultured trophoblast cells. This inhibitory effect of glucose on PGH secretion was dose-dependent. More than 50% inhibition being observed with 5.5 mmol/L. In the same conditions, the daily production of hPL and hCG, were unmodified. Furthermore, the glucose-induced inhibition of PGH secretion was more effective when cultured trophoblast cells are differentiated into syncytiotrophoblast. This study demonstrates, for the first time, that among the gestational polypeptide hormones secreted by the human placenta, only PGH secretion is modulated by glucose, suggesting a key metabolic role for this hormone during pregnancy. [less ▲]

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See detailFemoral bone density and vitamin D deficiency in elderly women
DEROISY; Pirenne, H; Frederick, I et al

in Archives of Public Health (1995), 52(S1), 81

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See detailUn cimetière, une communauté, un espace : l’exemple liégeois
Schmitz, Serge ULg

in Géographie et cultures (1995), 16

The article is the result of the study of 160 years of cemetery history in an urban region which houses today 510.000 peoples. It demonstrates the strong links between urban and rural communities and ... [more ▼]

The article is the result of the study of 160 years of cemetery history in an urban region which houses today 510.000 peoples. It demonstrates the strong links between urban and rural communities and their cemeteries. In order to do this, four points in cemetery history, chosen for the availability of reliable and comparable sources, have been chosen and described successively. Finally the article investigates the reasons for the exponential growth of requiered cemetery size. [less ▲]

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See detailCeci est mon corps. Een inleiding
Spinoy, Erik ULg

in Yang : Tijdschrift voor Literatuur en Kommunikatie (1995), 31(5), 4-10

This is an introduction to a special issue on the body in art and literature.

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See detailMiospore Biozonation in the Middle Devonian - Lower Carboniferous of Europe : its application to Western Gondwana
Loboziak, S.; Streel, Maurice ULg

in Anais da Academia Brasileira de Ciencias (1995), 67(3), 311-312

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See detailDe ruimtebeleving in Vestdijks Verzamelde verhalen III
Nachtergaele, Lutgarde ULg

in Vestdijkkroniek (1995), 88-89

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See detailCoexpression of cytokeratin and vimentin in eutopic endometrium and endometriosis throughout the menstrual cycle: evaluation by a computerized method.
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Donnez, Jacques

in Fertility and Sterility (1995), 64(1), 69-75

Objectives: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was ... [more ▼]

Objectives: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was carried out using the new computerized technology of image analysis. SETTING: University Hospital, Department of Gynecology. PATIENTS: Biopsies were taken from patients undergoing a laparoscopy for infertility (29 endometrial biopsies and 31 biopsies of peritoneal endometriosis). None of them were treated. RESULTS: Cyclic variations of cytokeratin and vimentin staining were noted in eutopic endometrium. The cytokeratin and vimentin staining pattern consistently was lower in ectopic endometrium than in eutopic endometrium. CONCLUSIONS: Endometriotic epithelial and stromal cells undergo a complex program of differentiation giving them histochemical characteristics similar to those observed in endometrium. Such a concomitant expression of antigenicity indicates their close relationship with their mesodermal müllerian origin. [less ▲]

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See detailStudies On The In-Situ Nitrogen Degradability Corrected For Bacterial-Contamination Of Concentrate Feeds In Steers
Beckers, Yves ULg; Thewis, André ULg; Maudoux, B. et al

in Journal of Animal Science (1995), 73(1), 220-227

Stable 15N was used to evaluate the influence of bacterial contamination on in situ DM and N degradabilities (Dg) of meat and bone meal (MBM), soybean meal (SBM), and wheat bran (WB) in two steers ... [more ▼]

Stable 15N was used to evaluate the influence of bacterial contamination on in situ DM and N degradabilities (Dg) of meat and bone meal (MBM), soybean meal (SBM), and wheat bran (WB) in two steers. Bacterial DM and N contamination ranged from 2.4 to 28.6% and 2.1 to 56.8% of residual DM and N, respectively. Effective N degradability increased when bacterial contamination was taken into account ( P < .05). The difference was low for MBM (2.4%) and for SBM (3.4%) but high for WB (12.2%). Theoretically, using solid-associated bacteria should give the most accurate correction for bacterial contamination; however, results showed that Dg of N based on liquid-associated bacteria were identical for MBM and SBM ( P > .05) and different for WB ( P .05). In a second experiment, five treatments were applied to incubated feeds to remove bacteria fxed to the residues and consequently to determine directly the Dg of DM and N corrected for the bacterial contamination without the need for a marker. These treatments involved chilling for 6 h at 4°C in saline solution alone ( T l ) or with a commercial detergent (T2), or with sodium dodecyl sulfate (T3) or with methylcellulose (T4), followed by pummeling in a stomacher for 5 min. The last treatment was only machine washing twice (T5). The Dg of DM can be directly determined following the first four treatments, nevertheless their application to MBM and SBM led to higher Dg of N than that corrected for the bacterial contamination determined in the frst experiment ( P < .05). This overestimation was higher than the observed underestimation when the correction for bacterial contamination was omitted. However, two treatments (T1 and T4) were able to dislodge bacterial N fixed to incubated WB and so the Dg of N could be measured directly. In conclusion, bacterial contamination of concentrate feeds incubated in the rumen may be extensive. In this case Dg must be corrected for this contamination or treatments for decontamination can be applied to residues before determining the Dg of N. [less ▲]

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See detailDe réels progrès ont-ils été faits dans le traitement de l'ostéoporose ?
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Franchimont, P

in Medi-Sphere (1995), 39

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See detailRemote Sensing and Urban Change
Donnay, Jean-Paul ULg; Barnsley, Mike

in GISDATA Newsletter (1995), 6

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See detailAcquired Persistent Atypical Lentigines as a Failure of 5-Methoxypsoralen-Containing Sunscreens in the Photochemoprotection from Ultraviolet-Radiation-Induced Damage
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Nikkels, Arjen ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1995), 190(4), 338-340

Acquired persistent atypical lentigines are pigmentary lesions that are unusual both clinically and microscopically. They occur in young adults with photosensitive skin using 5-methoxypsoralen-containing ... [more ▼]

Acquired persistent atypical lentigines are pigmentary lesions that are unusual both clinically and microscopically. They occur in young adults with photosensitive skin using 5-methoxypsoralen-containing sunscreens during sunbathing. Their biologic behavior is benign, but there are grounds for concern regarding the carcinogenic risk in these patients. [less ▲]

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See detailR-HuEPO hyporesponsiveness - who and why ?
Danielson, B.; Beguin, Yves ULg; Bommer, J. et al

in Nephrology Dialysis Transplantation (1995), 10

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See detailQuelques alternatives à la régression classique dans le cas de la colinéarité
Palm, Rodolphe ULg; Iemma, A. F.

in Revue de Statistique Appliquée (1995), XLIII(2), 5-33

In this note, we briefly describe some biased regresion methods : principal component regression, latent root regression analysis, partial least square regression, ridge regression and use of James-Stein ... [more ▼]

In this note, we briefly describe some biased regresion methods : principal component regression, latent root regression analysis, partial least square regression, ridge regression and use of James-Stein's estimators. All these methods are illustrated by an example. [less ▲]

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See detailNumerical simulation of a full scale fire test on a loaded steel framework
Franssen, Jean-Marc ULg; Cooke, C. M. E.; Latham, D. J.

in Journal of Constructional Steel Research (1995), 35

A single bay single storey steel portal frame has been tested under fire conditions. It is here simulated using hte non linear computer code CEFICOSS. The elements have composite steel-concrete sections ... [more ▼]

A single bay single storey steel portal frame has been tested under fire conditions. It is here simulated using hte non linear computer code CEFICOSS. The elements have composite steel-concrete sections for the thermal analysis, but only the steel part of the sections is load bearing. [less ▲]

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See detailComparative assessment of right ventricular performance from the pressure-volume relationship in double-muscled and conventional calves.
Amory, Hélène ULg; Mc Entee, Kathleen ULg; Linden, Annick ULg et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1995), 59(2), 135-141

Forty-one and 55 records of right-sided and systemic arterial pressures, cardiac output, and end-diastolic and end-systolic right ventricular volumes were collected from a group of 6 conventional and 6 ... [more ▼]

Forty-one and 55 records of right-sided and systemic arterial pressures, cardiac output, and end-diastolic and end-systolic right ventricular volumes were collected from a group of 6 conventional and 6 double-muscled calves, respectively. In each group, the mean right ventricular pressure-volume loop was constructed. Global cardiac performance was significantly lower in the double-muscled than in the conventional calves. The right ventricular end-diastolic and end-systolic volumes, as well as the diastolic portion of the mean pressure-volume loop, were similar in the 2 groups. Those results suggest that the reduced cardiac performance of double-muscled calves is not due to a lowered ventricular preload and that diastolic properties of their myocardium are similar to those of conventional calves. When expressed on a body weight basis, however, the right ventricular end-diastolic and end-systolic volumes were lower in the double-muscled than in conventional calves. When expressed as a function of probable metabolic demand, therefore, the volumetric capacity of the cardiac pump appears to be reduced in double-muscled calves. The significantly lower right ventricular ejection fraction, maximal rate of ventricular pressure rise and right ventricular peak-systolic pressure to end-systolic volume ratio measured in double-muscled as compared with conventional calves suggest that reduced myocardial contractility may also be partly responsible for the significantly lower stroke index of the former calves. The cardiac pump of double-muscled cattle thus seems to be less effective than that of conventional cattle because of reduced volumetric capacity and lowered strength of contraction. [less ▲]

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See detailAmibiase intestinale sévère d'origine européenne?
Carme, Bernard; Viot, J.; Hayette, Marie-Pierre ULg et al

in Médecine et Maladies Infectieuses (1995), 25(5),

A case of severe amebic colitis in a 21 year-old french patient is described. It raises the problems of modality and place of contamination, Entamoeba histolytica levels of virulence and differenciation ... [more ▼]

A case of severe amebic colitis in a 21 year-old french patient is described. It raises the problems of modality and place of contamination, Entamoeba histolytica levels of virulence and differenciation between E. histolytica and E. dispar. [less ▲]

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See detailAneurysms of the abdominal aorta: familial and genetic aspects in three hundred thirteen pedigrees.
VERLOES, Alain ULg; SakalihasanN, Natzi ULg; Koulischer, Lucien ULg et al

in Journal of Vascular Surgery (1995), 21(4), 646-55

PURPOSE: Familial clustering of abdominal aortic aneurysm was first noticed in 1977. METHODS: Through questionnaire and phone inquiry, familial data on 324 probands with abdominal aortic aneurysms allowed ... [more ▼]

PURPOSE: Familial clustering of abdominal aortic aneurysm was first noticed in 1977. METHODS: Through questionnaire and phone inquiry, familial data on 324 probands with abdominal aortic aneurysms allowed the establishment of 313 multigenerational pedigrees including 39 with multiple affected patients. RESULTS: There were 276 sporadic cases (264 men, 12 women); 81 cases belonged to multiplex pedigrees (76 men; 5 women). We compared familial and sporadic male cases; the ages at diagnosis were 64.1 +/- 7.9 years and 66.0 +/- 7.3 years (p < 0.05), respectively, the ages at rupture were 65.4 +/- 6.6 years and 75.2 +/- 8.6 years (p < 0.001), and the rupture rate was 32.4% and 8.7% (p < 0.001). Survival curves were computed. Relative risk for male siblings of a male patient was 18. We performed a segregation analysis with the mixed model, the most likely explanation for occurrence of abdominal aortic aneurysm in our families was a single gene effect showing dominant inheritance. The frequency of the morbid allele was 1:250, and its age-related penetrance was not higher than 0.4. CONCLUSION: This analysis indicates the preeminence of genetic factors on multifactorial/environmental effects of the pathogenesis of abdominal aortic aneurysm. [less ▲]

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See detailThiamine Deficiency in Cultured Neuroblastoma Cells: Effect on Mitochondrial Function and Peripheral Benzodiazepine Receptors
Bettendorff, Lucien ULg; Goessens, Guy ULg; Sluse, Francis ULg et al

in Journal of Neurochemistry (1995), 64(5), 2013-2021

When neuroblastoma cells were transferred to a medium of low (6 nM) thiamine concentration, a 16-fold decrease in total intracellular thiamine content occurred within 8 days. Respiration and ATP levels ... [more ▼]

When neuroblastoma cells were transferred to a medium of low (6 nM) thiamine concentration, a 16-fold decrease in total intracellular thiamine content occurred within 8 days. Respiration and ATP levels were only slightly affected, but addition of a thiamine transport inhibitor (amprolium) decreased ATP content and increased lactate production. Oxygen consumption became low and insensitive to oligomycin and uncouplers. At least 25% of mitochondria were swollen and electron translucent. Cell mortality increased to 75% within 5 days. [3H]PK 11195, a specific ligand of peripheral benzodiazepine receptors (located in the outer mitochondrial membrane) binds to the cells with high affinity (KD = 1.4 +/- 0.2 nM). Thiamine deficiency leads to an increase in both Bmax and KD. Changes in binding parameters for peripheral benzodiazepine receptors may be related to structural or permeability changes in mitochondrial outer membranes. In addition to the high-affinity (nanomolar range) binding site for peripheral benzodiazepine ligands, there is a low-affinity (micromolar range) saturable binding for PK 11195. At micromolar concentrations, peripheral benzodiazepines inhibit thiamine uptake by the cells. Altogether, our results suggest that impairment of oxidative metabolism, followed by mitochondrial swelling and disorganization of cristae, is the main cause of cell mortality in severely thiamine-deficient neuroblastoma cells. [less ▲]

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See detailMolecular description of the interactions of aminoglycoside antibiotics with negatively-charged phospholipids. Theoretical molecular modelling and experimental results.
Mingeot-Leclercq, M. P.; Schanck, A.; Van Bambeke, F. et al

in Pharmacology (1995), 14

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See detailCryptocrine Signaling in the Thymus Network and T Cell Education to Neuroendocrine Self-Antigens
Geenen, Vincent ULg; Goxe, Béatrice; Martens, Henri ULg et al

in Journal of Molecular Medicine : Official Organ of the 'Gesellschaft Deutscher Naturforscher und Ärzte' (1995), 73(9), 449-55

Both during phylogeny and ontogeny the thymus appears as a nodal point between the two major systems of cell-to-cell signaling, the neuroendocrine and immune systems. This review presents the experimental ... [more ▼]

Both during phylogeny and ontogeny the thymus appears as a nodal point between the two major systems of cell-to-cell signaling, the neuroendocrine and immune systems. This review presents the experimental observations which support a dual role in T cell selection played by the thymic repertoire of neuroendocrine polypeptide precursors. Through the mode of cryptocrine intercellular signaling thymic neuroendocrine-related precursors synthesized in thymic epithelial cells have been shown to influence the early steps in T cell differentiation. In addition, thymic neuroendocrine-related polypeptides are a source of self-antigens which are presented by the major histocompatibility system of the thymic epithelium. Preliminary data also suggest that the intrathymic T cell education to neuroendocrine self-antigens is not strictly superimposible to the antigen presentation by dedicated presenting cells. Insulin-like growth factor-II (IGF-II) was identified as one dominant member of the insulin family expressed by thymic epithelial and nurse cells. The intrathymic presentation of IGF-II or IGF-II derived self-antigens is under current investigation. If further confirmed, the central tolerogenic properties of IGF-II could be considered in the elaboration of a strategy for an efficient and safe prevention of insulin-dependent diabetes. [less ▲]

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See detailConformational-Analysis Of The Calcium Complexes Formed By Meglitinide Analogs
Lins, Laurence ULg; Brasseur, Robert ULg; Malaisse, Wj.

in Research Communications In Molecular Pathology and Pharmacology (1995), 90(1), 153-64

The conformation of calcium complexes formed with meglitinide and its hypoglycemic analogs KAD-1229, A-4166, S 3075 and repaglinide was analyzed by a semi-empirical procedure, which includes calculation ... [more ▼]

The conformation of calcium complexes formed with meglitinide and its hypoglycemic analogs KAD-1229, A-4166, S 3075 and repaglinide was analyzed by a semi-empirical procedure, which includes calculation of the molecular hydrophobicity potential. The calcium complexes formed with KAD-1229, A-4166, repaglinide or its enantiomer, displayed favourable attributes to act as ionophores. Such was not the case for the poorly efficient insulinotropic agent meglitinide and the biologically inactive enantiomer of A-4166. The configuration of the calcium complex formed with S 3075 also failed to display the features required for a high ionophoretic capacity. [less ▲]

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See detailBiology and clinical uses of erythropoietin in infants and children
Beguin, Yves ULg

in International Journal of Pediatric Hematology/Oncology (1995), 2

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See detail11-(4-methylpiperazin-1-yl)-5H-pyrido[4,3-b][1,5]benzodiazepine
Dupont, L.; Liégeois, Jean-François ULg; Rogister, F. et al

in Acta Crystallographica Section C-Crystal Structure Communications (1995), C51

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See detailTroubles du rythme et risques somatiques chez le petit enfant
Gauthier, Jean-Marie ULg

in Thérapie Psychomotrice et Recherches (1995), 102

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See detailCytotoxicity towards human endothelial cells, induced by neutrophil myeloperoxidase: protection by ceftazidime.
Mathy, Marianne ULg; Deby, Ginette ULg; Deby, C. et al

in Mediators of Inflammation (1995), 4(6), 437-43

We investigated the effects of the antibiotic ceftazidime (CAZ) on the cytolytic action of the neutrophil myeloperoxidase-hydrogen peroxide-chloride anion system (MPO/H(2)O(2)/Cl(-)). In this system ... [more ▼]

We investigated the effects of the antibiotic ceftazidime (CAZ) on the cytolytic action of the neutrophil myeloperoxidase-hydrogen peroxide-chloride anion system (MPO/H(2)O(2)/Cl(-)). In this system, myeloperoxidase catalyses the conversion of H(2)O(2) and CI(-) to the cytotoxic agent HOCl. Stimulated neutrophils can release MPO into the extracellular environment and then may cause tissue injury through direct endothelial cells lysis. We showed that human umbilical vein endothelial cells (HUVEC) were capable of taking up active MPO. In presence of H(2)O(2) (10(-4) M), this uptake was accompanied by cell lysis. The cytolysis was estimated by the release of (51)Cr from HUVEC and expressed as an index of cytotoxicity (IC). Dose dependent protection was obtained for CAZ concentrations ranging from 10(-5) to 10(-3) M;this can be attributed to inactivation of HOCl by the drug. This protection is comparable to that obtained with methionine and histidine, both of which are known to neutralize HOCl. This protection by CAZ could also be attributed to inactivation of H(2)O(2), but when cytolysis was achieved with H(2)O(2) or O(2) (-) generating enzymatic systems, no protection by CAZ was observed. Moreover, the peroxidation activity of MPO (action on H(2)O(2)) was not affected by CAZ, while CAZ prevented the chlorination activity of MPO (chlorination of monochlorodimedon). So, we concluded that CAZ acts via HOCl inactivation. These antioxidant properties of CAZ may be clinically useful in pathological situations where excessive activation of neutrophils occurs, such as in sepsis. [less ▲]

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See detailDisfuncion ventricular en la infeccion HIV perinatal
Valdes, Eduardo F; Fernandez Rostello, Eduardo; Romero Villanueva, Horacio et al

in Prensa Medica Argentina (1995), 82

We studied cardiovascular function in four babies . There were three females (3, 6 and 9 months old ) and one 6 months old male with HIV perinatal infection (ELISA and Western blot positives). CD4 counts ... [more ▼]

We studied cardiovascular function in four babies . There were three females (3, 6 and 9 months old ) and one 6 months old male with HIV perinatal infection (ELISA and Western blot positives). CD4 counts were: 1150/mm3, 1250 /mm3, 950/mm3 and 1066/mm3 respectively. The first baby had pneumocystosis and oral candidiasis, and two other had bacterial pneumonia and viral brochiolitis as antecedents. We observed after echocardiographic Doppler evaluation right ventricle diastolic dysfunction in three cases. Interestingly there were no pulmonary hypertension. Diastolic and systolic left ventricle function was normal in all babies. Our previous results in 27 AIDS adult patients let us to identify mainly left ventricle dysfunction (Valdes E & al Prensa Medica Argentina 1994). On the contrary, right ventricle dysfunction was predominant in this small series. Lipshultz & al (Am J Cardio 1990) found in 31 perinatal AIDS ventricular dysfunction, myocardial dilatation, arrhythmias and pericardial effusion. Our results combined with a literature review point out cardiac dysfunction as a frequent finding in children with AIDS. As these alterations are not necessarily clinically apparent, we recommend routine echocardiographic evaluation in these patients. Physiopathological clues in AIDS cardiac dysfunction remain elusive: HIV cytotoxic myocardiopathy, opportunistic infections and/or viral autoimmune mechanisms have been considered as potential causes. [less ▲]

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See detail2,4,7-Trimethyl-2,3-dihydro-4H-pyrido[4,3-e]-1,2,4-thiadiazinium 1,1-dioxide iodide
Dupont, L.; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Acta Crystallographica (1995), C51

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See detailThe continuum of cytokine involvement in the physiology of reproduction
Geenen, Vincent ULg; Hazee, Marie-Thérèse ULg; Goxe, Béatrice et al

in GENESIS (1995), 14

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See detailCytokines and cartilage degradation
Henrotin, Yves ULg; De Groote, D; Labasse, A et al

in Mediators of Inflammation (1995), 4

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See detailGalectin-3, a Laminin Binding Protein, Fails to Modulate Adhesion of Human Melanoma Cells to Laminin
van den Brule, F. A.; Buicu, C.; Sobel, M. E. et al

in Neoplasma (1995), 42(5), 215-9

Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of ... [more ▼]

Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and A375 melanoma cell expressed galectin-3 on their surface as demonstrated by immunofluorescence, and attached to laminin in an in vitro assay. We demonstrate that neither recombinant galectin-3 nor an affinity purified antigalectin-3 antiserum altered adhesion of A2058 or A375 melanoma cells to laminin. Our data strongly suggest that galectin-3 is not a key element in adhesion of the melanoma cells to laminin. These results are not surprising in light of the observation that galectin-3 expression is down regulated in cancer and that increased adhesion to laminin is a constant feature of invasive cancer cells. [less ▲]

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See detailCombined radiotherapy and radioimmotherapy of human colon carcinoma grafted in nude mice.
Buchegger, F; Rojas, A; Delaloye, A et al

in Cancer Research (1995), 55

The effect of combined radioimmunotherapy (RIT) and fractionated external beam radiotherapy (RT) was assessed in two human colon cancer xenografts, Col 12 and LS174T in nude mice. These tumors were ... [more ▼]

The effect of combined radioimmunotherapy (RIT) and fractionated external beam radiotherapy (RT) was assessed in two human colon cancer xenografts, Col 12 and LS174T in nude mice. These tumors were selected for being resistant to RIT alone, as is usually the case in the clinical situation. Tumor-bearing mice were treated with a combination of five X-ray fractions over 5 days followed by RIT with two doses of 1.5 mCi 131I-labeled anticarcinoembryonic antigen monoclonal antibody F(ab')2. In Col 12 and LS174T, RIT alone achieved a regrowth delay similar to that of fractionated RT with total doses of 28 and 26 Gy, respectively. In both tumor types, an additive therapeutic effect, measured as increased regrowth delay or local control, was observed when combining RT of different dose levels with RIT. Normal tissue responses were assessed by monitoring acute peak skin reactions and blood cell count. Bone marrow depression for the combination treatment was similar to that of RIT alone; relative to skin, at equitoxic levels, no mice bearing Col 12 tumors were locally controlled with a 32 Gy RT dose alone, while this RT combined with RIT gave a local control of 100%. These studies show a therapeutic benefit when external beam RT is combined with RIT. [less ▲]

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See detailCancers épithéliaux de l'ovaire. Acquisitions récentes
van den Brule, F. A.; Lambotte, René ULg; Castronovo, Vincenzo ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1995), 24(3), 241-52

Ovarian carcinomas constitute the major cause of the mortality and morbidity in gynaecology. Most ovary carcinomas are epithelial tumours. Our understanding of ovarian cancerogenesis has been hampered by ... [more ▼]

Ovarian carcinomas constitute the major cause of the mortality and morbidity in gynaecology. Most ovary carcinomas are epithelial tumours. Our understanding of ovarian cancerogenesis has been hampered by the lack of a well defined precursor lesion, the lack of knowledge about tumour progression, and by the relative inaccessibility of the ovaries in the abdominal cavity. Recent studies using experimental models allow us to better define the fundamental mechanisms of carcinogenesis from the serous ovarian cells and of invasion of the abdominopelvic cavity by proximity. This review article tries to update on epidemiology, genetic syndromes, biology, screening, and therapy of these epithelial tumours, and about the new directions taken by basic and clinical research. We will present data concerning oncogenes and tumour suppressor genes involved in epithelial ovarian tumours, regulation of tumour cells by growth factors, genes involved in tumour invasion, and mechanisms used by the cancer cell to resist to therapies. Non-epithelial ovarian tumours will not be examined in this manuscript. [less ▲]

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See detailThe effectiveness of combined oral lysine acetylsalicylate and metoclopramide compared with oral sumatriptan for migraine.
Tfelt-Hansen, P.; Henry, P.; Mulder, L. J. et al

in Lancet (1995), 346(8980), 923-6

Aspirin is commonly used to treat migraine attacks, although sumatriptan, a much more expensive treatment, is also effective. We compared a combination of lysine acetylsalicylate (equivalent to 900 mg ... [more ▼]

Aspirin is commonly used to treat migraine attacks, although sumatriptan, a much more expensive treatment, is also effective. We compared a combination of lysine acetylsalicylate (equivalent to 900 mg aspirin) and 10 mg metoclopramide (LAS+MTC) with oral sumatriptan (100 mg) and placebo in 421 patients with migraine. LAS+MTC was as effective as sumatriptan with a decrease of headache from severe or moderate to mild or none of 57% and 53%, respectively, for the first migraine attack treated. Both treatments were better than placebo (success rate 24%, p < 0.0001). LAS+MTC was significantly more effective in the treatment of nausea than sumatriptan (p < 0.0001) and was better tolerated (adverse events in 18% and 28%, respectively, p < 0.05). LAS+MTC is as effective as sumatriptan in the treatment of migraine attacks. It is also much cheaper. [less ▲]

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See detailProductivité et efficacité dans l'assurance
Fecher-Bourgeois, Fabienne ULg; Pestieau, Pierre ULg

in Risques - Les Cahiers de l'assurance (1995), 22(Avril-juin 1995), 65-71

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See detailneuroendocrinology in the perinatal period
Battisti, Oreste ULg

in mini acta of belgian society of pediatrics (1995), 27

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See detailExtracellular matrix proteins and basement membrane identification in bovine ovaries and significance for the attachment of cultured preantral follicles
Figueiredo, J. R.; Hulshof, S. C. J.; Thiry, Marc ULg et al

in Theriogenology (1995), 43(5), 845-858

Described in the present paper is the immunolocalization of the extracellular matrix proteins (e.g., fibronectin, collagen Types I and III) in the bovine ovary, with special attention to preantral ... [more ▼]

Described in the present paper is the immunolocalization of the extracellular matrix proteins (e.g., fibronectin, collagen Types I and III) in the bovine ovary, with special attention to preantral follicles. In addition, we have shown, histochemically and ultrastructurally, that mechanically isolated bovine preantral follicles are surrounded by an intact basement membrane. After 24 h of culture in serum-free medium, only 20.4% of these follicles attached to a plastic substrate. We showed that covering the plastic with extracellular matrix proteins (i.e., fibronectin, collagen Type I and matrigel) significantly increased the percentage of attached follicles to 76.0, 65.2 and 80.4%, respectively, while laminin had no effect (18.6%). When preantral follicles were embedded within three-dimensional collagen gels, no loss of follicles was observed. Restoring surface interactions between preantral follicles and the extracellular matrix in vitro, either in a two- or a three-dimensional system, might be important for maintaining follicular viability and growth in the future. [less ▲]

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See detailNumber and relative size of thenar motor units estimated by an adapted multiple point stimulation method.
Wang, François-Charles ULg; Delwaide, Paul ULg

in Muscle & nerve (1995), 18(9), 969-79

An adapted multiple point stimulation (AMPS) method is described for estimating the number and relative size of thenar motor units. With this method, the median nerve was stimulated at various sites from ... [more ▼]

An adapted multiple point stimulation (AMPS) method is described for estimating the number and relative size of thenar motor units. With this method, the median nerve was stimulated at various sites from the wrist to the elbow. To avoid alternation, only two or three clearly identifiable surface-recorded motor unit action potentials (S-MUAPs) were recruited at each point by incremental stimulation. A total of 10 S-MUAPs, elicited from four to five distinct stimulation points, was used to calculate the average S-MUAP size. By dividing the maximum M-potential size by that value, a motor unit number estimate (MUNE) was derived. In 59 healthy volunteers, from 19 to 87 years old, the mean average S-MUAP size was 87 +/- 27.6 microV.ms and the mean MUNE was 278 +/- 113 motor units. When performed repeatedly, the results were reproducible. The number of motor units declined exponentially with age while average S-MUAP sizes increased only moderately. To assess the validity of the AMPS method, its results were correlated with those obtained using the F-response technique. The correlation coefficient was 0.83 (P < 0.001). [less ▲]

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See detailDe Karmelietenkerk in Gent, Dendrochronologische analyse van de dakconstructie boven tweede beuk
Hoffsummer, Patrick ULg; Houbrechts, David

in Stadsarcheologie (1995), 18(2),

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See detailComment j'explore... Une anemie (1e partie).
Beguin, Yves ULg

in Revue Médicale de Liège (1995), 50(10), 443-4

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See detailBreakdown of the stereospecificity of DD-peptidases and beta-lactamases with thiolester substrates.
Damblon, Christian ULg; Zhao, G. H.; Jamin, M. et al

in Biochemical Journal (1995), 309 ( Pt 2)

With peptide analogues of their natural substrates (the glycopeptide units of nascent peptidoglycan), the DD-peptidases exhibit a strict preference for D-Ala-D-Xaa C-termini. Gly is tolerated as the C ... [more ▼]

With peptide analogues of their natural substrates (the glycopeptide units of nascent peptidoglycan), the DD-peptidases exhibit a strict preference for D-Ala-D-Xaa C-termini. Gly is tolerated as the C-terminal residue, but with a significantly decreased activity. These enzymes were also known to hydrolyse various ester and thiolester analogues of their natural substrates. Some thiolesters with a C-terminal leaving group that exhibited L stereochemistry were significantly hydrolysed by some of the enzymes, particularly the Actinomadura R39 DD-peptidase, but the strict specificity for a D residue in the penultimate position was fully retained. These esters and thiolesters also behave as substrates for beta-lactamases. In this case, thiolesters exhibiting L stereochemistry in the ultimate position could also be hydrolysed, mainly by the class-C and class-D enzymes. However, more surprisingly, the class-C Enterobacter cloacae P99 beta-lactamase also hydrolysed thiolesters containing an L residue in the penultimate position, sometimes with a higher efficiency than the D isomer. [less ▲]

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See detailLe méthotrexate en rhumatologie: une révolution thérapeutique
Kaye, O.; Ribbens, Clio ULg; Hauwaert, Christian ULg et al

in Revue Médicale de Liège (1995), 50(11), 453-457

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See detailLa disparition de l'absorption
Kefer, Fabienne ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (1995)

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See detailCharacteristics of the pulsatile liberation of LH in Italian Simmental cows from the dry period to early lactation
Prandi, Alberto; Motta, Mario; Rossi, A. et al

in Bulletin des Recherches Agronomiques de Gembloux (1995), 30

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See detailA magnetic resonance imaging approach for the diagnosis of a triplet cornual pregnancy.
Bassil, Salim; Gordts, Stéphane; NISOLLE, Michelle ULg et al

in Fertility and Sterility (1995), 64(5), 1029-31

Objectives: To describe a rare case of triplet cornual pregnancy after IVF-ET and to assess the role of magnetic resonance imaging (MRI) for early diagnosis. DESIGN: Case report. SETTING: Infertility and ... [more ▼]

Objectives: To describe a rare case of triplet cornual pregnancy after IVF-ET and to assess the role of magnetic resonance imaging (MRI) for early diagnosis. DESIGN: Case report. SETTING: Infertility and IVF Unit, in an university medical center. PATIENT: A 31-year-old healthy patient with a 3-year history of primary male infertility. INTERVENTION: Standard IVF-ET treatment cycle, using a GnRH-agonist (long protocol) and hMG for ovarian stimulation. RESULTS: A cornual triplet pregnancy is described with the approach for the diagnosis. Magnetic resonance imaging was complementary to endovaginal sonography for early diagnosis and permitted a timed conservative management. CONCLUSIONS: When transvaginal ultrasound findings did not provide an accurate location of the pregnancy, MRI was indicated to confirm the diagnosis. [less ▲]

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See detailObservations on the mites (Acarai) associated with Carabidae (Coleoptera) in Belgium.
Fain, A.; Noti, J.; Dufrêne, Marc ULg

in International Journal of Acarology (1995), 21

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See detailAntihyperglycaemic agents. Drug interactions of clinical importance.
Scheen, André ULg; Lefebvre, Pierre ULg

in Drug Safety : An International Journal of Medical Toxicology & Drug Experience (1995), 12(1), 32-45

Non-insulin-dependent (type 2) diabetes mellitus (NIDDM) affects middle-aged or elderly people who frequently have several other concomitant diseases, especially obesity, hypertension, dyslipidaemias ... [more ▼]

Non-insulin-dependent (type 2) diabetes mellitus (NIDDM) affects middle-aged or elderly people who frequently have several other concomitant diseases, especially obesity, hypertension, dyslipidaemias, coronary insufficiency, heart failure and arthropathies. Thus, polymedication is the rule in this population, and the risk of drug interactions is important, particularly in elderly patients. The present review is restricted to the interactions of other drugs with antihyperglycaemic compounds, and will not consider the mirror image, i.e. the interactions of antihyperglycaemic agents with other drugs. Oral antihyperglycaemic agents include sulphonylureas, biguanides--essentially metformin since the withdrawn of phenformin and buformin--and alpha-glucosidase inhibitors, acarbose being the only representative on the market. These drugs can be used alone or in combination to obtain better metabolic control, sometimes with insulin. Drug interactions with antihyperglycaemic agents can be divided into pharmacokinetic and pharmacodynamic interactions. Most pharmacokinetic studies concern sulphonylureas, whose action may be enhanced by numerous other drugs, thus increasing the risk of hypoglycaemia. Such an effect may result essentially from protein binding displacement, inhibition of hepatic metabolism and reduction of renal clearance. Reduction of the hypoglycaemic activity of sulphonylureas due to pharmacokinetic interactions with other drugs appears to be much less frequent. Drug interactions leading to an increase in plasma metformin concentrations, mainly by reducing the renal excretion or the hepatic metabolism of the biguanide, should be avoided to limit the risk of hyperlactaemia. Owing to its mode of action, pharmacokinetic interferences with acarbose are limited to the gastrointestinal tract, but have not been extensively studied yet. Pharmacodynamic interactions are quite numerous and may result in a potentiation of the hypoglycaemic action or, conversely, in a deterioration of blood glucose control. Such interactions may be observed whatever the type of antidiabetic treatment. They result from the intrinsic properties of the coprescribed drug on insulin secretion and action, or on a key step of carbohydrate metabolism. Finally, a combination of 2 to 3 antihyperglycaemic agents is common for treating patients with NIDDM to benefit from the synergistic effect of compounds acting on different sites of carbohydrate metabolism. Possible pharmacokinetic interactions between alpha-glucosidase inhibitors and classical antidiabetic oral agents should be better studied in the diabetic population. [less ▲]

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See detailDENSITY-POLARIZATION FUNCTIONAL THEORY OF THE RESPONSE OF A PERIODIC INSULATING SOLID TO AN ELECTRIC-FIELD
GONZE, X.; Ghosez, Philippe ULg; GODBY, R. W.

in Physical Review Letters (1995), 74(20), 4035-4038

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See detailFra Stein til Waldrop: sprogdigtningen og de nye prosadigtere
Delville, Michel ULg; Schatz-Jakobsen, Claus

in Den Blå Port: Tidsskrift for Litteratur (1995)

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See detailA Propos de la Physique du Ruissellement sur Bassin Versant
Pirotton, Michel ULg

in Tribune de l'Eau (La) (1995), 48(573), 3-10

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See detailAcquis de la recherche en matière d'évaluation génétique des ovins et des caprins en Tunisie
D'Jemali, M'naouer; Jamal, Sofiene; Ben-Dhiaf, Sonia et al

in Cahiers Options Méditerranéennes (1995), 11

Work carried out on performance recording for growth and milk yield in sheep and goats for use in genetic evaluation is briefly summarized, and preliminary data on the growth and reproductive traits of 9 ... [more ▼]

Work carried out on performance recording for growth and milk yield in sheep and goats for use in genetic evaluation is briefly summarized, and preliminary data on the growth and reproductive traits of 9 flocks of Barbary sheep in 1987-92 are presented in 14 tables and discussed. [less ▲]

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See detailMeasurement of glucosinolates in rapeseeds.
Wathelet, Jean-Paul ULg; Marlier, M.; Severin, M. et al

in Natural toxins (1995), 3(4),

Several ring tests have been organized by the International Organisotion for Standardization (lSO) and the Measurement ônd Testing Programme (BCR) for improving glucosinolote methods Finally, HPLC of ... [more ▼]

Several ring tests have been organized by the International Organisotion for Standardization (lSO) and the Measurement ônd Testing Programme (BCR) for improving glucosinolote methods Finally, HPLC of desulphoglucosinolates is recommended by lSO, the European Committee for Standardizotion (CEN), and the European Commission (EC) as the official method. X-ray fluorescence has also become particularly common for fast analysis. After checking stability of intact glucosinolates in whole rapeseeds, the BCR has three reference materials (CRM 366, CRM 190, CRM 367) avai able, certified for their total glucosinolate and sulphur contents. Currently, the Measurement and Testing Programme is supporting a research project concerning the stdbility of desulphoglucosinolates and intact glucosinolates extracts. At the present time, best results were observed with lyophilized extracts sealed in brown glass ampoules and stored at 18'C. [less ▲]

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See detailIndice plasmodique et parasitémie à Plasmodium falciparum: valeur diagnostique et pronostique au Congo.
Carme, B.; Hayette, Marie-Pierre ULg; Mbitsi, A. et al

in Annales de la Société Belge de Médecine Tropicale (1972) (1995), 75

Parasitological data of various malarial studies performed in the Congo where Plasmodium falciparum malaria is holoendemic in rural and suburban zones between 1988 and 1991, were analyzed with the ... [more ▼]

Parasitological data of various malarial studies performed in the Congo where Plasmodium falciparum malaria is holoendemic in rural and suburban zones between 1988 and 1991, were analyzed with the intention of establishing diagnosis and prognosis value of Plasmodium falciparum parasitaemia in areas with high perennial transmission. In such an area Congolese school children (6-10 years old) had 88% P. falciparum index, this is the same percentage as that for children hospitalized with a pernicious attack. However, the parasite load is distributed differently; parasitaemia is greater than 6,000 asexual form for P. falciparum (af/Pf/µl) in only 4,6% of cases in the former group, versus 67% in the second group. A threshold of 10,000 af/Pf/µl above which the Plasmodium infection triggers a febrile attack in semi-immune children, is confirmed in school children in a rural context where the factor of taking antimalarial drugs within the preceding days is negligible: three out of four children with levels above this threshold are febrile versus 4,1% (7 out of 170) with lower blood parasite levels. Some adults where also asymptomatic carriers but much less frequently and with lower mean parasitaemia levels. The parasite load mirrors the clinical severity although this concept can be misleading as an individual prognostic criterion and foor hospital studies carried out in areas where multiple drug administration before hospitalization is common. For the studies recently performed in Brazzaville, the 5% threshold of parasitized red cells, the WHO severity criterion was never reached in asymptomatic subject or in cases of simple attack; it was reached in one out of two cases of pernicious attack. [less ▲]

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See detailLaparoscopic Intrauterine Insemination in the Bitch
Silva, L. D.; Onclin, K.; Snaps, Frédéric ULg et al

in Theriogenology (1995), 43(3), 615-23

A technique for laparoscopic intrauterine insemination in bitches is described. During natural estrus, 5 beagle bitches were inseminated and S others were naturally mated (control group) twice at a 48-h ... [more ▼]

A technique for laparoscopic intrauterine insemination in bitches is described. During natural estrus, 5 beagle bitches were inseminated and S others were naturally mated (control group) twice at a 48-h interval on Days 3 and S (n = 4) or Days 4 and 6 (n = 6) after the increase in plasma progesterone considered to be indicative of the day of the preovulatory LH peak. All the inseminations were with fresh semen and under general anesthesia. The technique involved the introductions of 1) a Verres needle to insufflate the abdominal cavity by direct punction on the middle line 1 cm over the umbilicus, 2) a laparoscope to visualize the abdominal cavity by a 1 cm puncture on the middle line 1 cm under the umbilicus, 3) a forceps used to manipulate the uterus by a 0.5 cm puncture at 2 to 3 cm lateral to the mammary glands, and 4) an 18-g catheter used to puncture the uterus on the middle line between the 3rd and 5th mammary gland. The uterine body was grasped by the forceps and elevated against the ventral abdominal wall. The 18-g catheter was then inserted through the abdominal wall directly into the uterine lumen, and 1.0 ml of fresh semen containing 250 to 480 x 10(6) spermatozoa/ml was injected. The inseminations resulted in pregnancies in all animals. Litter size was similar in the artificially inseminated and naturally mated bitches (5 +/- 1.8 and 4.8 +/- 1.6 pups per litter, respectively). Bitches in the artificially inseminated group delivered at 65.2 +/- 0.8 d and in the natural mated group at 65.4 +/- 0.5 d after the LH peak. In conclusion, this paper gives the first results of intrauterine laparoscopic insemination in bitches, indicating interesting perspectives for this technique in dog's reproduction. [less ▲]

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See detailNF kappa B and interferon regulatory factor 1 physically interact and synergistically induce major histocompatibility class I gene expression.
Drew, P. D.; Franzoso, G.; Becker, K. G. et al

in Journal of Interferon & Cytokine Research (1995), 15(12), 1037-45

Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has ... [more ▼]

Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has remained unclear. We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence (ICS). We further demonstrated that NF kappa B and IRF-1 physically interacted with each other and cooperatively induced MHC class I gene expression when cotransfected into CHP-126 neuroblastomas. These results provide a molecular mechanism by which TNF-alpha and IFN-gamma synergistically induce the expression of a variety of genes involved in immune responses, including MHC class I. [less ▲]

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See detailPulmonary Ventilation, Mechanics, Gas Exchange and Haemodynamics in Calves Following Intratracheal Inoculation of Pasteurella Haemolytica
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in Zentralblatt für Veterinarmedizin. Reihe A (1995), 42(8), 531-544

A Pasteurella haemolytica A1 broth was injected intratracheally in eight calves and measurements of pulmonary function values (PFV) were made once before and hourly post inoculation (p.i.). Changes in ... [more ▼]

A Pasteurella haemolytica A1 broth was injected intratracheally in eight calves and measurements of pulmonary function values (PFV) were made once before and hourly post inoculation (p.i.). Changes in PFVs, included increased respiratory rate and minute ventilation (up to 158% of baseline 2 h p.i.) and decreased tidal volume and lung dynamic compliance (up to 33% of baseline 3 h p.i.). Total pulmonary resistance was not affected. At and after 3 h p.i. there was a progressive impairement of gas exchange, as judged from arterial O2 tension which decreased up to 65% of baseline. In contrast, arterial CO2 tension was not affected. Pulmonary hypertension was observed during the 3 last h of the study and was attributable to an increased pulmonary vascular resistance. Severe neutropenia was observed at 3 h p.i. and post-mortem histological findings were consistent with an acute fibrinohemorragic bronchopneumonia. In conclusion, P. haemolytica airway challenge unequiovocally resulted in acute pneumonia, providing a reproducible pathophysiological model for investigations regarding new therapeutic strategies. [less ▲]

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See detailLe determinisme genetique de la differenciation sexuelle humaine.
Ectors, Fabien ULg

in Revue Médicale de Bruxelles (1995), 16(6), 404-10

The genetic mechanism controlling sexual differentiation had remained unknown for a long time. Karyotype analysis of sex-inverted patients or individuals with ambiguous sexual differentiation has enabled ... [more ▼]

The genetic mechanism controlling sexual differentiation had remained unknown for a long time. Karyotype analysis of sex-inverted patients or individuals with ambiguous sexual differentiation has enabled the localization and identification of genes involved. It is currently known that the SRY gene is responsible for the initiation of a cascade reaction leading to male differentiation of the primitive gonad. SRY is a +/- 820 base pairs gene located on the small arm of the Y chromosome, more precisely within the 1A1 alpha sub-segment. Although a few other genes are known to be involved in the downstream regulation of SRY, their precise mode of action is yet unknown. [less ▲]

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See detailHysteroscopic myomectomy.
Donnez, Jacques; Polet, Roland; Smets, Mireille et al

in Current Opinion in Obstetrics & Gynecology (1995), 7(4), 311-6

Transhysteroscopic myomectomy has proved to be safe and effective with experienced operators. The use of the continuous flow hysteroscope and preoperative monitoring of intrauterine pressure has ... [more ▼]

Transhysteroscopic myomectomy has proved to be safe and effective with experienced operators. The use of the continuous flow hysteroscope and preoperative monitoring of intrauterine pressure has contributed to the prevention of fluid intravasion accidents. Effectiveness has been demonstrated in a number of recently published long-term studies. [less ▲]

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See detailUtilisation des matières grasses chez les bovins à l'engraissement
Clinquart, Antoine ULg; Micol, Didier; Brundseaux, Christophe et al

in Productions Animales (1995), 8

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See detailAssessment of equine diaphragm strength and activation using transvenous phrenic nerve stimulation
Desmecht, Daniel ULg; Linden, Annick ULg; Lekeux, Pierre ULg

in Equine Veterinary Journal. Supplement (1995), 18

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See detailLack of LTR and ENV genetic variation during bovine leukemia virus-induced leukemogenesis.
Willems, Luc ULg; Kerkhofs, P.; Burny, A. et al

in Virology (1995), 206(1),

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See detailCharles Grandgagnage (Liège 1812-Liège 1878)
Boutier, Marie-Guy ULg

in Wallonnes : Chronique de la Société de Langue et de Littérature Wallonnes (1995), (4), 4

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See detailSituation actuelle de la loutre, Lutra lutra, en Belgique et problématique de sa conservation
Libois, Roland ULg; Hallet, Catherine

in Cahiers d'Ethologie (1995), 15(2-3-4), 157-168

Widespread all over the country till the end of the last century, the otter has rapidly declined. In 1973 when hunting was definitely closed, the otter was already threatened and restricted to a few ... [more ▼]

Widespread all over the country till the end of the last century, the otter has rapidly declined. In 1973 when hunting was definitely closed, the otter was already threatened and restricted to a few localities. In 1889, the Belgian Ministry of agriculture decided to pay bounties for otters, a system which was cancelled only in 1963. Game statistics of the past century allow the annual otter bag to evaluated as roughly 25 % of the standing population. As it is the case in other European industrialised countries, freshwater habitat loss and water pollution lead to a considerable decrease of the river carrying capacity. Almost all the rivers flowing in the Northern part of Belgium are now lifeless or at least in a very bad condition. South of the river Meuse, some accidental pollutions are regularly reported (chloring in the river Ourthe in 1993; chloracetic acid in the Sûre in 1990...) and the level of organic pollution rises from year to year. Moreover, the tourism (angling, camp sites, kayak) nare more and more intensive leading to major disturbances, particularly during the summer months. Finally, the Ministery of equipment is developing a policy of big hydraulic works (dams, embankments, dredging...) along the river Ourthe, one of the last to be occupied by a few otters. [less ▲]

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See detailTEM1 beta-lactamase structure solved by molecular replacement and refined structure of the S235A mutant.
Fonze, E.; Charlier, Paulette ULg; To'th, Y. et al

in Acta Crystallographica Section D-Biological Crystallography (1995), 51(Pt 5), 682-94

beta-Lactamases are bacterial enzymes which catalyse the hydrolysis of the beta-lactam ring of penicillins, cephalosporins and related compounds, thus inactivating these antibiotics. The crystal structure ... [more ▼]

beta-Lactamases are bacterial enzymes which catalyse the hydrolysis of the beta-lactam ring of penicillins, cephalosporins and related compounds, thus inactivating these antibiotics. The crystal structure of the TEM1 beta-lactamase has been determined at 1.9 A resolution by the molecular-replacement method, using the atomic coordinates of two homologous beta-lactamase refined structures which show about 36% strict identity in their amino-acid sequences and 1.96 A r.m.s. deviation between equivalent Calpha atoms. The TEM1 enzyme crystallizes in space group P2(1)2(1)2(1) and there is one molecule per asymmetric unit. The structure was refined by simulated annealing to an R-factor of 15.6% for 15 086 reflections with I >/= 2sigma(I) in the resolution range 5.0-1.9 A. The final crystallographic structure contains 263 amino-acid residues, one sulfate anion in the catalytic cleft and 135 water molecules per asymmetric unit. The folding is very similar to that of the other known class A beta-lactamases. It consists of two domains, the first is formed by a five-stranded beta-sheet covered by three alpha-helices on one face and one alpha-helix on the other, the second domain contains mainly alpha-helices. The catalytic cleft is located at the interface between the two domains. We also report the crystallographic study of the TEM S235A mutant. This mutation of an active-site residue specifically decreases the acylation rate of cephalosporins. This TEM S235A mutant crystallizes under the same conditions as the wild-type protein and its structure was refined at 2.0 A resolution with an R value of 17.6%. The major modification is the appearance of a water molecule near the mutated residue, which is incompatible with the OG 235 present in the wild-type enzyme, and causes very small perturbations in the interaction network in the active site. [less ▲]

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See detailInverse Modulation of Intraepithelial Langerhans' Cells and Stromal Macrophage/Dendrocyte Populations in Human Papillomavirus-Associated Squamous Intraepithelial Lesions of the Cervix
Al-Saleh, Walid; Delvenne, Philippe ULg; Arrese Estrada, Jorge ULg et al

in Virchows Archiv : An International Journal of Pathology (1995), 427(1), 41-8

Ninety-four cervical biopsies from normal tissue to high-grade squamous intraepithelial lesion (SILs) were examined for the presence of intraepithelial Langerhans' cells and subpopulations of stromal ... [more ▼]

Ninety-four cervical biopsies from normal tissue to high-grade squamous intraepithelial lesion (SILs) were examined for the presence of intraepithelial Langerhans' cells and subpopulations of stromal macrophages/dendrocytes by immunohistochemistry using anti-S100, -L1, -CD68 and -factor XIIIa antibodies. Human papillomavirus (HPV) detection was performed in all cases by using first a mixture of DNA probes for 14 HPV types commonly found in anogenital biopsies at low stringency conditions (Tm -40 degrees C) and by reanalyzing the tissues at high stringency (Tm -10 degrees C) with HPV 6/11, 16/18 and 31/33/35 biotinylated probe cocktails and individual digoxigenin-labelled probes. SILs and metaplastic tissues were significantly associated with a depletion of S100-positive intraepithelial Langerhans' cells when compared with normal epithelium. In contrast, there was a significant increase in L1-positive stromal macrophages in SIL biopsies compared with normal or metaplastic cervix. A significantly higher density of CD68-positive macrophages was also observed in high-grade SILs compared with normal or metaplastic biopsies and with low-grade SILs. The density of factor XIIIa-positive dendrocytes was found to be higher in SILs compared with metaplastic tissues and in high-grade SILs when compared with normal cervical biopsies. No specific relationship was found between the densities of these cells and the HPV type detected in SILs separated into low grade and high grade. The significance of this inverse modulation of intraepithelial Langerhans' cells and stromal macrophages/dendrocytes in normal and SIL biopsies is discussed in relation to HPV infection and malignant transformation. [less ▲]

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See detailExperimental production of hypocalcemia by EDTA infusion in calves : a critical appraisal assessed from the profile of blood chemicals and enzymes
Desmecht, Daniel ULg; Linden, Annick ULg; Godeau, J. M. et al

in Comparative Biochemistry and Physiology. A : Comparative Physiology (1995), 110A(2), 115-130

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See detailPacemaker Implantation for Early Sinus Node Dysfunction after Orthotopic Heart Transplantation
RADERMECKER, Marc ULg; Defraigne, Jean-Olivier ULg; Fourny, J. et al

in Acta Chirurgica Belgica (1995), 95(1), 31-4

Among 60 patients who received OHT at our institution between November 1986 and January 1990, 4 actually needed implantation of a permanent pacemaker. Three patients with symptomatic early sinus node ... [more ▼]

Among 60 patients who received OHT at our institution between November 1986 and January 1990, 4 actually needed implantation of a permanent pacemaker. Three patients with symptomatic early sinus node dysfunction were identified and were satisfactorily paced on the VVIR mode. All three showed sinus recovery within three months following implantation and had their pacemaker switched from the VVIR mode to a simple ventricular demand pacing. Interestingly, administration of beta-blocking drugs quickly reinstituted pace dependence. The patients' outcome is excellent (18 to 48 months follow-up). No difference with the global cohort of our OHT recipients was noticed, apart from an apparent high incidence of infections (3/3) with (2/3) CMV and severe rejection (3/3) during the first month postoperatively. This data suggests that sinus dysfunction may be the result of a multifactorial (rejection, CMV, ...) injury to the conduction system, and is only clinically relevant beyond a threshold level. Denervation hypersensitivity, together with correction of these factors, may account for the restoration of a sufficient reserve of conduction system, and therefore appropriate sinus node function in basal conditions. [less ▲]

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See detailSpecific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.
Weynants, V.; Godfroid, J.; Limbourg, B. et al

in Journal of Clinical Microbiology (1995), 33(3), 706-12

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of ... [more ▼]

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of cytoplasmic proteins from Brucella melitensis B115 was used as a specific antigenic stimulus in bovine whole blood culture. Supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (IFN-gamma) content by using a commercial sandwich enzyme-linked immunosorbent assay kit. The IFN-gamma assay was evaluated with 10 heifers during the course (80 days) of an experimental infection and with 14 cows from an ongoing brucellosis outbreak. All of these animals were slaughtered, and pertinent organs were subjected to classical bacteriological analyses. In addition, we analyzed 23 field cases in which false-positive serological reactions occurred. The IFN-gamma results were compared with those of the standard DTH and a battery of serological assays, and they were correlated with bacteriological data. Both for the experimental infection and for the field brucellosis outbreak, the IFN-gamma assay detected infection in more animals than any combination of the serological tests, and it detected infection earlier than these tests. Finally, none of the samples from cows showing false-positive serological reactions was classified as positive by the IFN-gamma assay, attesting to its specificity and to its usefulness in interpreting ambiguous serological results. A rapid and convenient alternative to the DTH, the IFN-gamma assay appears to be an ideal method that is complementary to the serological diagnosis protocols. [less ▲]

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See detailNF-kappaB transcription factor activation by hydrogen peroxide can be decreased by 2,3-dihydroxybenzoic acid and its ethylester derivative
Sappey, Christine; Boelaert, J. R.; Legrand-Poels, Sylvie ULg et al

in Archives of Biochemistry & Biophysics (1995)

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See detailDas Siegburger Kurbuch. Original und Übersetzung
Herborn, Wolfgang; Möller, Robert ULg

in Heimatblätter des Rhein-Sieg-Kreises (1995), 63

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See detailTreatment of Bone in Elderly Subjects: Calcium, Vitamin D, Fluor, Bisphosphonates, Calcitonin
Reginster, Jean-Yves ULg

in Hormone Research (1995), 43(1-3), 83-8

Several approaches may be suggested for prevention or treatment of senile osteoporosis. In elderly patients, an association of calcium and vitamin D supplementation has been shown to reduce nonvertebral ... [more ▼]

Several approaches may be suggested for prevention or treatment of senile osteoporosis. In elderly patients, an association of calcium and vitamin D supplementation has been shown to reduce nonvertebral fractures up to 40% over an 18-month period. Calcitonin, given parenterally or nasally, may prevent further bone loss and has been reported to significantly decrease vertebral and hip fractures. This hormone also possesses an important analgesic effect. Fluoride salts are the most effective way to significantly increase bone mass, mainly at the trabecular site. The optimal doses and regimens remain to be clearly established. The use of new formulations like sodium monofluorophosphate are likely to facilitate the search of the optimal therapeutic window. Bisphosphonates are potent inhibitors of bone resorption. They have been shown to significantly increase trabecular bone mass without impairment of cortical bone. Their use may be associated with a significant decrease in vertebral fractures, mainly in patients with severe osteoporosis. The new, second and third generation of bisphosphonates (tiludronate, risedronate, alendronate) look extremely promising but their widespread use will only be possible after a confirmation of the absence of a deleterious effect on bone quality (alendronate) and after demonstration of a reduction in vertebral and/or hip fractures following a prolonged use. [less ▲]

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See detailGerminal Center T Cells: Analysis of Their Proliferative Capacity
Bouzahzah, F.; Bosseloir, A.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1995), 378

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See detailEvaluacion del comportamiento agressivo en ninos preescolares
Born, Michel ULg; Egido, A.; Pire, B.

in Revista Galega de Psicopedagogia (1995), 12(8), 149-165

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See detailAmylin/islet amyloid polypeptide: biochemistry, physiology, patho-physiology.
Castillo, M. J.; Scheen, André ULg; Lefebvre, Pierre ULg

in Diabète & Métabolisme (1995), 21(1), 3-25

Amylin is a 37 amino-acid peptide mainly produced by the islet beta-cell. Aggregation of amylin is partly responsible for amyloid formation. Amyloid deposits occur both extracellularly and intracellularly ... [more ▼]

Amylin is a 37 amino-acid peptide mainly produced by the islet beta-cell. Aggregation of amylin is partly responsible for amyloid formation. Amyloid deposits occur both extracellularly and intracellularly and may contribute to beta-cell degeneration. Amylin is packed in beta-cell granules and cosecreted with insulin in response to the same stimuli but, unlike other beta-cell products, it is produced from specific a gene on chromosome 12. Basal, plasma amylin concentrations are around 5 pM, and increase fourfold after meals or glucose. Higher levels are found in cases of insulin resistance, obesity, gestational diabetes and in some patients with NIDDM. Low or absent levels are found in insulin-dependent diabetic patients. There are similarities between amylin and non beta-cell peptides such as calcitonin gene related peptides (CGRP). They may bind to the same receptor, determine similar post-receptor phenomena and qualitatively similar actions but with different degree of potency. The actions of amylin are multiple and mostly exerted in the regulation of fuel metabolism. In muscle, amylin opposes glycogen synthesis, activates glycogenolysis and glycolysis (increasing lactate production). Consequently, amylin increases lactate output by muscle and increases the plasma lactate concentration. In fasting conditions, this lactate may serve as a gluconeogenic substrate for the liver, contributing to replenish depleted glycogen stores and to increase glucose production. In non-fasting conditions, lactate can be transformed by liver in triglycerides. It is not clear at present whether amylin actions on the liver are direct or mediated by changes in circulating metabolites. A probably indirect effect of amylin in muscle is to decrease insulin- (or glucose)-induced glucose uptake, which may contribute to insulin resistance. Other actions include inhibition of glucose-stimulated insulin secretion and, in general, actions mimicking CGRP effects. Some of these actions are seen at supraphysiological concentrations. The physiopathological consequences of amylin deficiency, or excess are under active by investigated. [less ▲]

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See detailTreatment of patent ductus arteriosus by placement of intravascular coils in a pup
Snaps, Frédéric ULg; Mc Entee, Kathleen ULg; Saunders, J. et al

in Journal of the American Veterinary Medical Association [=JAVMA] (1995), 6(6), 724-725

A 3-month-old female mixed-breed pup was referred for evaluation and treatment of patent ductus arteriosus (PDA). Selective angiography was performed to confirm the diagnosis. A cone-shaped PDA with a ... [more ▼]

A 3-month-old female mixed-breed pup was referred for evaluation and treatment of patent ductus arteriosus (PDA). Selective angiography was performed to confirm the diagnosis. A cone-shaped PDA with a large aortic base was outlined. A second selective angiographic procedure was performed to functionally close the PDA by use of thrombotic coils; complete obstruction was obtained. Twenty-four hours after the procedure, the murmur disappeared. One month later, the dog was reexamined and found to be clinically normal. [less ▲]

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See detailLes fondements de l'analyse numérique de photos prises au fisheye pour des besoins topoclimatologiques
Cornélis, Bernard ULg; Erpicum, Michel ULg

in Publications de l'Association Internationale de Climatologie (1995), 8

Fisheye pictures are frequently used in forestry and in ecology for canopy measurements. In climatology, it seems that they have only been used for illustration purposes. In fact, fisheye pictures are ... [more ▼]

Fisheye pictures are frequently used in forestry and in ecology for canopy measurements. In climatology, it seems that they have only been used for illustration purposes. In fact, fisheye pictures are rich in climatological information. Digital fisheye image processing can be used for applied climatology as well as for topoclimatological studies. It allows the quantification of insolation, diffuse radiation, sky-view factor,.… In other words, it provides an objective assessment of the exposure of a site or of a measuring device. This article introduces the basis for digital fisheye image processing. The possibilities offered by this tool will be illustrated with sunshine measurements in an urban environment. [less ▲]

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See detailISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
ELLING, Lothar; GULDENBERG, Birgit; GROTHUS, Marita et al

in Biotechnology & Applied Biochemistry (1995), 21(Part 1), 29-37

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the ... [more ▼]

The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time [less ▲]

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See detailPharma clinics. Comment je traite ... une anemie (3e partie).
Beguin, Yves ULg

in Revue Médicale de Liège (1995), 50(12), 497-8

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See detailUtilisation de la paille dans des rations d'engraissement: ingestion, dynamique de la digestion et cinétique de dégradabilité dans le rumen
Mayombo, Asangule Pierre; Van Eenaeme, Christian ULg; Gauthier, Sabine et al

in Annales de Zootechnie (1995), 44

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See detailOverexpression of a novel member of the mitochondrial carrier family rescues defects in both DNA and RNA metabolism in yeast mitochondria.
Van Dyck, E.; Jank, B.; Ragnini, A. et al

in Molecular & General Genetics [=MGG] (1995), 246

The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast ... [more ▼]

The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast Saccharomyces cerevisiae. A multicopy suppressor capable of rescuing the respiratory deficient phenotype associated with null alleles of either gene has been isolated. This suppressor is a nuclear gene that was called RIM2/MRS12. The RIM2/MRS12 gene encodes a predicted protein of 377 amino acids that is essential for mitochondrial DNA metabolism and proper cell growth. Inactivation of this gene causes the total loss of mitochondrial DNA and, compared to wild-type rhoo controls, a slow-growth phenotype on media containing glucose. Analysis of the RIM2/MRS12 protein sequence suggests that RIM2/MRS12 encodes a novel member of the mitochondrial carrier family. In particular, a typical triplicate structure, where each repeat consists of two putative transmembrane segments separated by a hydrophilic loop, can be deduced from amino acid sequence comparisons and the hydropathy profile of RIM2/MRS12. Antibodies directed against the aminoterminus of RIM2/MRS12 detect this protein in mitochondria. The function of the RIM2/MRS12 protein and the substrates it might transport are discussed. [less ▲]

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