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See detailUltrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1993), 205(2), 430-2

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The ... [more ▼]

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleolar body until in close contact with the fibrillar centers. Morphometric analysis reveals that 32% of the fibrillar center surface is essentially occupied by condensed chromatin. Using the in situ terminal deoxynucleotidyl transferase-immunogold procedure for detecting DNA, we further show that evident label is exclusively found over the condensed chromatin and over the fibrillar centers, whereas no significant label is detected over the dense fibrillar component of the nucleolus. [less ▲]

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See detailTextual Space in Un chien Andalou
Drummond, Philippe; Van Cauwenberge, Geneviève ULg

in La revue belge du cinéma (1993), 33-35

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See detailLocalisation scintigraphique de tumeurs neuroendocrines et de leurs métastases par un analogue de la somatostatine: [111In-DTPA-d-phe1] octréotide.
Beckers, Albert ULg; Pham, V. T.; Abs, R. et al

in Revue Médicale de Liège (1993), 48(6), 326-34

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See detailPENICILLIN-BINDING PROTEIN 2X OF STREPTOCOCCUS-PNEUMONIAE - ENZYMATIC-ACTIVITIES AND INTERACTIONS WITH BETA-LACTAMS
JAMIN, M.; Damblon, Christian ULg; MILLIER, S. et al

in Biochemical Journal (1993), 292(Part 3), 735-741

The high-molecular-mass penicillin-binding protein (PBP) 2x, one of the primary targets of beta-lactam antibiotics in Streptococcus pneumoniae, has been produced as a soluble form and purified in large ... [more ▼]

The high-molecular-mass penicillin-binding protein (PBP) 2x, one of the primary targets of beta-lactam antibiotics in Streptococcus pneumoniae, has been produced as a soluble form and purified in large amounts. It has been shown to catalyse hydrolysis and transfer reactions with different ester and thiolester substrates and its catalytic behaviour was often similar to that of the soluble DD-peptidase from Streptomyces R61. This provided an easy method to monitor the activity of the PBP. For the first time, a reliable kinetic study of the interaction between a lethal target and beta-lactam antibiotics has been performed. Characteristic kinetic parameters were obtained with different beta-lactam compounds. These results not only validated the mechanism established with non-essential extracellular enzymes, but will also constitute the basis for comparative studies of the low-affinity variants from penicillin-resistant strains. [less ▲]

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See detailLa collaboration entre les CPAS et autres institutions d'Aide Sociale
Gavray, Claire ULg; Huvelle, P.

in Observatoire : Revue d'Action Sociale & Médico-Sociale (1993), 1-2

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See detailA finite element code for subsidence problems: LAGAMINE
Charlier, Robert ULg; Radu, Jean-Pol ULg; Li, Q. F.

in Bulletin of Engineering Geology & the Environment (1993), 47(1 / avril), 5-11

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See detailTYPE OF WEAR FOR THE PAIR TI6A14V/PCTFE IN AMBIENT AIR AND IN LIQUID-NITROGEN
Bozet, Jean-Luc ULg

in Wear (1993), 162(Part B), 1025-1028

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V ... [more ▼]

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V/polychlorotrifluoroethylene (PCTFE) on a pin-on-disk tribometer in ambient air and liquid nitrogen with the contact pressure and sliding speed ranging, respectively, from 3 to 9 MPa and 0.03 to 0.05 m s-1. Because of the affinity of titanium for halogen elements, such as chlorine and fluorine, which are both present in PCTFE, we noticed the formation of an abrasive compound made of chlorine, fluorine, titanium and aluminium. These elements were detected by electron microscopy. In both environments the heat produced during sliding is responsible for the decomposition of the PCTFE. The surface of the polymer is softened by the heat and embedded abrasive particles then scratch the sliding track of the Ti6Al4V disk. This abrasiveness is confirmed with a three-dimensional stylus profilometer used on the Ti6Al4V disks. The low temperature and neutrality of liquid nitrogen hardly inhibit the decomposition of the polymer. This is related to the poor thermal conductivity of both titanium and PCTFE: the heat produced at the contact is fully available for the chemical reaction between the chlorine, fluorine and titanium, all the more so since this reaction requires little energy to take place. A better understanding of the wear behaviour of the pair PCTFE/Ti6Al4V in both air and liquid nitrogen should lead to the development of a surface treatment which prevents the formation of abrasive particles. We could then use titanium alloys as bulk materials for sliding parts in cryogenic valves and, because of the low specific mass of titanium, could benefit from the subsequent weight reduction. [less ▲]

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See detailControl of the illegal administration of natural steroid hormones in urine and tissues of veal calves and in plasma of bulls
Scippo, Marie-Louise ULg; Gaspar, Pol; Degand, Guy ULg et al

in Analytica Chimica Acta (1993), 275(1-2), 57-74

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See detailAbility Of Amitraz To Maintain The Predator Exochomus-Quadripustulatus L (Col, Coccinellidae) In An Integrated Management Of Epulvinaria-Hydrangeae (Steinw) (Hom, Coccidae)
Tondeur, R.; Merlin, J.; Schiffers, Bruno ULg et al

in Journal of Applied Entomology-Zeitschrift Fur Angewandte Entomologie (1993), 115(1),

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See detailContingent negative variation: methods and potential interest in headache.
Schoenen, Jean ULg; Timsit-Berthier, M.

in Cephalalgia : An International Journal of Headache (1993), 13(1), 28-32

Contingent negative variation (CNV) is an event-related slow cerebral potential which has been found abnormal in migraine. Its methodology is described. Contrary to other neurophysiological techniques ... [more ▼]

Contingent negative variation (CNV) is an event-related slow cerebral potential which has been found abnormal in migraine. Its methodology is described. Contrary to other neurophysiological techniques, CNV needs special equipment and expertise. On average, CNV amplitude is increased and its habituation is lacking in migraine without aura between attacks, but not in migraine with aura. The sensitivity of CNV as a diagnostic tool is low, but its specificity is high. CNV amplitude normalizes after treatment with beta-blockers. The CNV abnormalities in migraine might be due to hyperreactivity of central catecholaminergic pathways. [less ▲]

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See detailResistance a la secheresse et reponses a l'acide abscissique: analyse d'une approche synthetique.
El-Jaafari, S.; Paul, Roger ULg; Lepoivre, Philippe ULg et al

in Cahiers Agricultures (1993), 2(4),

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See detailMolecular Biology of tilapia prolactins
Swennen, E.; Sekkali, B.; Poncelet, A. C. et al

in Fundamental and applied research for aquaculture (1993)

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See detailThe central role of the thymus in the education of T cells to neuroendocrine self principles
Geenen, Vincent ULg; Martens, Henri ULg; Cormann-Goffin, Nadine et al

in Archives Internationales de Physiologie et de Biochimie (1993), 101

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See detailStructure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution
Noble, M. E.; Zeelen, J. P.; Wierenga, R. K. et al

in Acta Crystallographica Section D-Biological Crystallography (1993), 49(Pt 4), 403-17

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 ... [more ▼]

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 A resolution with a crystal grown by the technique of hanging-drop crystallization from a mother liquor containing the transition-state analogue 2-phosphoglycolate (2PG). As a search model in the molecular replacement calculations, the refined structure of TIM from Trypanosoma brucei, which has a sequence identity of 46% compared to the enzyme from E. coli, was used. An E. coli TIM crystal grown in the absence of 2PG, diffracting to 2.6 A resolution, was later obtained by application of the technique of macro-seeding using a seed crystal grown from a mother liquor without 2PG. The final 2.6 A model has a crystallographic R factor of 11.9%, and agrees well with standard stereochemical parameters. The structure of E. coli TIM suggests the importance of residues which favour helix initiation for the formation of the TIM fold. In addition, TIM from E. coli shows peculiarities in its dimer interface, and in the packing of core residues within the beta-barrel. [less ▲]

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See detailMesurer les compétences en lecture: Apports et limites d'une enquête internationale
Lafontaine, Dominique ULg

in Lettre de la DFLM (La) (1993), 12

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See detailDes inscriptions de sceaux-cylindres diverses provenant d'Acemhöyük
Tunca, Önhan ULg

in Aspects of Art and Iconography: Anatolia and its Neighbors. Studies in Honor of Nimet Özgüç (1993)

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See detailExperts et novices face à un circuit simulé sur ordinateur
Lafontaine, Dominique ULg; Blondin, Christiane ULg; Closset, Jean-Louis ULg

in Education et Recherche (1993), 1

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See detailInterface entre médecin de famille, référent social et psychiatrie: un hôpital de jour
Triffaux, Jean-Marc ULg; Halkin, Stéphane ULg; Bertrand, Jean ULg

in Observatoire : Revue d'Action Sociale & Médico-Sociale (1993)

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See detailDu bon steak et du bon lait: d'abord interroger le naturel et en percer tous ces secrets
Renaville, Robert ULg; Burny, Arsène; Portetelle, Daniel ULg

in Agricontact (1993), 255

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See detailEffect of a Booster Vaccination against Influenza and Equine Herpes Virus on Cardio-Respiratory Adjustments to Strenuous Exercise and Training in Thoroughbred Horses
Art, Tatiana ULg; Lekeux, Pierre ULg

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (1993), 40(7), 481-491

This study was conducted in order to assess whether exercise- and training-induced cardio-respiratory adjustments are modified during the 10-day period which follows a booster vaccination with an oily ... [more ▼]

This study was conducted in order to assess whether exercise- and training-induced cardio-respiratory adjustments are modified during the 10-day period which follows a booster vaccination with an oily adjuvanted inactivated vaccine against influenza and equine herpesvirus-1 (Equiffa). Nine healthy vaccinated thoroughbred horses were used. Six were revaccinated and three were kept as control. All the horses completed a standardised exercise test (SET) that was repeated 4 times, i.e. 10 (SET1) and 2 (SET2) days before revaccination, and 2 (SET3) and 10 (SET4) days after revaccination. During the whole experimental period the horses were trained 6 days per week according to an interval training schedule. Respiratory airflow, tidal volume, respiratory rate and expired minute volume (VE) were measured using a face mask and 2 ultra-sonic pneumotachographs. The oxygen uptake (VO2) and carbon dioxide production (VCO2) were obtained on a breath-by-breath basis, using a mass spectrometer. Heart rate (HR) was continuously measured with a polar horse tester. Venous blood was sampled before and after the test and analysed for biochemical determinations. Blood was also sampled before and 21 days after the booster vaccination for circulating antibody titration. The peak VO2, VCO2, ventilatory equivalent (VE/VO2) and oxygen pulse (VO2/HR) were significantly improved by the 3-week training period. The other cardio-respiratory parameters as well as most of the blood parameters remained unchanged throughout the 4 SETs. The revaccination did not impair any of the parameters measured before, during, or after the SETs. All revaccinated horses showed a rise in antibodies against influenza virus type A1 and A2 and EVH-1 compared to the control horses. It was concluded that, in our horses, revaccination with an oily adjuvanted inactivated vaccine against influenza and EVH-1 did not impair their cardiorespiratory and metabolic adjustments to strenuous exercise and intense training, and that the seroconversion due to revaccination was unaffected by the intense daily exercise experienced by the animals in the post-vaccination period [less ▲]

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See detailLe récepteur NMDA : base moléculaire du mécanisme d’action de la kétamine
Hanon, E.; Gustin, Pascal ULg; Ansay, M.

in Annales de Médecine Vétérinaire (1993), 137

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See detailReturn interval, firm size and systematic risk on the Dutch stock market
Corhay, Albert ULg; Tourani-Rad, Alireza

in Review of Financial Economics (1993), 2(2), 19-28

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See detailEvaluation isocinétique des genoux ligamentaires en pré et postopératoire
Croisier, Jean-Louis ULg; Delcour, J. P.; Dupont, Géraldine ULg et al

in Lettre de Médecine Physique et de Réadaptation (1993), 29

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See detailMathematical Programming Methods for Constrained Optimization: Dual Methods
Fleury, Claude ULg

in Progress in Astronautics and Aeronautics (1993), 150

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See detailThiamin and Derivatives as Modulators of Rat Brain Chloride Channels
Bettendorff, Lucien ULg; Hennuy, Benoît ULg; Wins, Pierre et al

in Neuroscience (1993), 52(4), 1009-1017

Several membrane fractions were prepared from rat brain by differential and sucrose density gradient centrifugation. Most fractions took up 36Cl- rapidly at a rate linear with time during the first 30-60 ... [more ▼]

Several membrane fractions were prepared from rat brain by differential and sucrose density gradient centrifugation. Most fractions took up 36Cl- rapidly at a rate linear with time during the first 30-60 s, then the rate progressively slowed down. The lowest rate of uptake was found in the mitochondrial fraction. Oxythiamin partially inhibited 36Cl- uptake in all fractions. In P2 (crude synaptosomal fraction), oxythiamin decreased the initial rate of uptake by 32%, the apparent Ki being 1.5 mM. Thiamin and amprolium were less effective as inhibitors. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (0.1-1 mM) inhibited 36Cl- uptake by 40-50%. In the presence of this compound at a concentration > or = 5 x 10(-4) M, oxythiamin became ineffective. 36Cl- uptake was increased by GABA (0.1 mM) and this effect was antagonized by picrotoxin as expected, but not by oxythiamin. The rate of 36Cl- uptake did not appreciably depend on the external chloride concentration and was unaffected by bumetanide or by replacement of external Na+ by choline. Taken together, these data suggest that the oxythiamin-sensitive 36Cl- influx is essentially diffusional and is not related to the GABA receptor or the Na:K:2Cl co-transport. Partial replacement of external Na+ by K+ or treatment with 0.1 mM veratridine (which should both result in membrane depolarization) increased 36Cl- uptake by 50 and 30% respectively; the inhibitory effect of oxythiamin was enhanced to the same proportion.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailPlasticity of Developing and Adult Dorsal Root Ganglion Neurons as Revealed in Vitro
Delree, P.; Ribbens, Clio ULg; Martin, Didier ULg et al

in Brain Research Bulletin (1993), 30(3-4), 231-7

We review recent data on the plasticity of dorsal root ganglion (DRG) neurons as revealed during cultivation in vitro. Some experiments on cultured developing DRG neurons and on adult DRG neurons in vivo ... [more ▼]

We review recent data on the plasticity of dorsal root ganglion (DRG) neurons as revealed during cultivation in vitro. Some experiments on cultured developing DRG neurons and on adult DRG neurons in vivo are also mentioned. Cultured developing and adult DRG neurons can be switched from an apolar to a multipolar phenotype by fetal calf serum or fibronectin. The effect is concentration dependent and occurs through an early modification of cell-substratum interaction. Adult DRG neurons synthesize and release within hours after injury TGF beta-1, which is a mitogen and a differentiation factor for Schwann cells. Finally, adult DRG neurons express in vitro neurotransmitters that are not expressed in vivo. This neurotransmitter plasticity can be modulated in vitro by some growth factors and in vivo by distal or proximal axotomy. [less ▲]

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See detailSYNCYTIOTROPHOBLASTIC LOCALIZATION OF THE HUMAN GROWTH-HORMONE VARIANT MESSENGER-RNA IN THE PLACENTA
Scippo, Marie-Louise ULg; Frankenne, Francis ULg; HOOGHEPETERS, ELisabeth et al

in Molecular & Cellular Endocrinology (1993), 92(2), 7-13

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental ... [more ▼]

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using P-32-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only. [less ▲]

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See detailDynamics of mitotic activity and expression of viral proteins gp51 and p24 of Bovine Leukemia Virus producing cells.
Roussev, Roussi; Polianova, M.; Portetelle, Daniel ULg et al

in Folia Biologica (1993), 39

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See detailViolences des jeunes : à l'école et ailleurs ?
Born, Michel ULg; Gavray, Claire ULg

in Observatoire : Revue d'Action Sociale & Médico-Sociale (1993), 6

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See detailCaractéristiques du nid et du site de nidification chez le grèbe huppé (Podiceps cristatus). Influence sur le succès des éclosions
Marechal, Corinne ULg

in Cahiers d'Ethologie (1993), 13(1), 1-34

The study aimed at determining the influence of nest and nest-site characteristics on breeding success in a population of great Crested grebes (Podiceps cristatus) nesting on an old máeander of the river ... [more ▼]

The study aimed at determining the influence of nest and nest-site characteristics on breeding success in a population of great Crested grebes (Podiceps cristatus) nesting on an old máeander of the river Meuse on the Belgium-Dutch border. Evolution of clutches has been followed during two breeding seasons (1991 and 1992). The features of the nests and the nest-sites have been subjected to a multivariate analysis. It’s difficult to distinguish clearly between successful and unsuccessful nests during the same year. Moreover, most characteristics are not constant any year. Only two parameters seem to have the same effects on nesting success in 1991 and 1992 : the distance from nest to nearest neighbour and the water level in nest site. Nests distant from another nest and in deep water were less successful. Other possible causes of nesting failure have been tackled (investigator disturbance, bad weather conditions, fall of eggs, clutch-destruction by human). [less ▲]

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See detailEl analisis estructural semi-rigido: definicion, camp y mood de applicacion
Jaspart, Jean-Pierre ULg; Maquoi, René ULg

in Construcciones Metalicas y Caldereria (1993), 28

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See detailMesures quantitatives de l'erythropoiese. Le recepteur soluble de la transferrine.
Beguin, Yves ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1993), 148(1-2), 100-4105-7

Iron transport in plasma is carried out by transferrin, which donates iron to cells through its interaction with a specific membrane receptor. The soluble form of the transferrin receptor has been ... [more ▼]

Iron transport in plasma is carried out by transferrin, which donates iron to cells through its interaction with a specific membrane receptor. The soluble form of the transferrin receptor has been identified in plasma. Dosage of the soluble receptor represents a simple, reliable, quantitative assay of erythropoiesis, which should replace the standard ferrokinetic measurement. Using this dosage, it is now possible to investigate the pathophysiology of anemia in many conditions, and to analyze the erythropoietic response to treatment with recombinant erythropoietin. [less ▲]

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See detailCloning of a human GHF-1/Pit-1 cDNA variant
Pernasetti, Flavia; Wera, Stefaan; Belayew, Alexandra et al

in Nucleic Acids Research (1993), 21(15), 3584

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See detailAnalyse des comportements d'entraîneurs de volley-ball pendant des rencontres officielles
Cloes, Marc ULg; Delhaes, Jean-Pierre; Piéron, Maurice

in Sport (1993), 141(1), 16-25

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se ... [more ▼]

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se regrouper selon leurs objectifs: (1) l'analyse descriptive des comportements d'entraîneurs et de sportifs, parfois de très haut niveau; (2) la comparaison de situations pédagogiques, notamment en observant une même personne exerçant les fonctions d'entraîner et d'enseignant; (3) la comparaison des interventions des entraîneurs en fonction du niveau d'habileté des sportifs qu'ils dirigent; (4) la comparaison du comportement des entraîneurs en fonction des succès ou des échecs de leur équipe. ... [less ▲]

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See detailEffect of the vector-axial-vector coupling on the vector-meson modes
Jaminon, Martine ULg; Mendez Galain, Ramon

in Nuclear Physics A (1993), 564

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See detailIn vitro susceptibility of Clostridium perfringens isolated from farm animals to growth-enhancing antibiotics.
Devriese, L. A.; Daube, Georges ULg; Hommez, J. et al

in Journal of Applied Bacteriology (1993), 75

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See detailModelling the Arterial Wall by Finite Elements
Mosora, F.; Harmant, A.; Hallet, Claude ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1993), 101(3, May-Jun), 185-91

The mechanical behaviour of the arterial wall was determined theoretically utilizing some parameters of blood flow measured in vivo. Continuous experimental measurements of pressure and diameter were ... [more ▼]

The mechanical behaviour of the arterial wall was determined theoretically utilizing some parameters of blood flow measured in vivo. Continuous experimental measurements of pressure and diameter were recorded in anesthetized dogs on the thoracic ascending and midabdominal aorta. The pressure was measured by using a catheter, and the diameter firstly, at the same site, by a plethysmograph with mercury gauge and secondly, by a sonomicrometer with ferroelectric ceramic transducers. The unstressed radius and thickness were measured at the end of each experiment in situ. Considering that the viscous component is not important relatively to the nonlinear component of the elasticity and utilizing several equations for Young modulus calculation (thick and thin wall circular cylindrical tube formulas and Bergel's equation) the following values were obtained for this parameter: 0.6 MPa-2 MPa in midabdominal aorta and 2 MPa-6.5 MPa in thoracic ascending aorta. The behaviour of the aorta wall was modelled considering an elastic law and using the finite element program "Lagamine" working in large deformations. The discretized equilibrium equations are non-linear and a unique axi-symmetric, iso-parametric element of 1 cm in length with 8 knots was used for this bi-dimensional problem. The theoretical estimation of radius vessel, utilizing a constant 5 MPa Young modulus and also a variable one, are in good agreement with the experimental results, showing that this finite element model can be applied to study mechanical properties of the arteries in physiological and pathological conditions. [less ▲]

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See detailSyngeneic Grafting of Adult Rat Drg-Derived Schwann Cells to the Injured Spinal Cord
Martin, Didier ULg; Schoenen, Jean ULg; Delree, P. et al

in Brain Research Bulletin (1993), 30(3-4), 507-14

A subdural inflatable micro-balloon was used to induce closed traumatic contusion to adult rat spinal cord. This spinal cord injury model was associated with reproducible and graded neurological deficits ... [more ▼]

A subdural inflatable micro-balloon was used to induce closed traumatic contusion to adult rat spinal cord. This spinal cord injury model was associated with reproducible and graded neurological deficits and histopathological alterations. At various delays after injury, transplantations of syngeneic adult cultured dorsal root ganglion-derived Schwann cells were performed into the spinal cord lesion. The transplants were well integrated and reduced the microcystic posttraumatic cavitation as well as the gliosis. Schwann cells transplants were invaded by numerous regenerating neurites most of which, based upon their neurotransmitter contents, seem to originate from the dorsal root ganglion. [less ▲]

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See detailLasfe (CIL IV 2319 l) et quelques autres exemples d’usage abusif de l’épigraphie en phonétique historique
Purnelle, Gérald ULg

in Etudes Classiques (Namur, Belgium) (1993), 61

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See detailOlfactory receptors are displayed on dog mature sperm cells
Vanderhaeghen, P.; Schurmans, Stéphane ULg; Vassart, G. et al

in Journal of Cell Biology (1993), 123

Olfactory receptors constitute a huge family of structurally related G protein-coupled receptors, with up to a thousand members expected. We have shown previously that genes belonging to this family were ... [more ▼]

Olfactory receptors constitute a huge family of structurally related G protein-coupled receptors, with up to a thousand members expected. We have shown previously that genes belonging to this family were expressed in the male germ line from both dog and human. The functional significance of this unexpected site of expression was further investigated in the present study. We demonstrate that a few dog genes representative of various subfamilies of olfactory receptors are expressed essentially in testis, with little or no expression in olfactory mucosa. Other randomly selected members of the family show the expected site of expression, restricted to the olfactory system. Antibodies were generated against the deduced amino acid sequence of the most abundantly expressed olfactory receptor gene in dog testis. The purified serum was able to detect the gene product (DTMT receptor) in late round and elongated spermatids, as well as in the cytoplasmic droplet that characterizes the maturation of dog sperm cells, and on the tail midpiece of mature spermatozoa. Western blotting further confirmed the presence of a 40-kD immunoreactive protein in the membrane of mature sperm cells. Altogether , these results demonstrate that the main expression site of a subset of the large olfactory receptor gene family is not olfactory mucosa but testis. This expression correlates with the presence of the corresponding protein during sperm cell maturation, and on mature sperm cells. The pattern of expression is consistent with a role as sensor for unidentified chemicals possibly involved in the control of mammalian sperm maturation, migration, and/or fertilization [less ▲]

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See detailSell-Off en de welvaart van aandeelhouders
Corhay, Albert ULg; Tourani-Rad, Alireza

in Maandblad voor Accountancy en Bedrijfseconomie [=MAB] (1993), 67(10), 471-477

In het kader van de herstructurering van het Nederlandse bedrijfsleven vindt de laatste jaren een groot aantal Sell-Offs plaats. Theoretici betogen dat een Sell-Off een aantal voordelen voor het ... [more ▼]

In het kader van de herstructurering van het Nederlandse bedrijfsleven vindt de laatste jaren een groot aantal Sell-Offs plaats. Theoretici betogen dat een Sell-Off een aantal voordelen voor het afstotende moederbedrijf oplevert. Blijkens deze studie ondersteunt het empirische onderzoek de theorie. In Nederland lijken significante buitengewone positieve koerseffecten op de dag van aankondiging voor de aandeelhouders van de verkopende bedrijven met dit soort transacties gepaard te gaan. Tevens is vastgesteld dat de aard van de aankondiging en de bekendmaking van het motief voor de Sell-Off positieve significante invloed heeft op de grootte van het buitengewoon rendement. Ten slotte is geconcludeerd dat alleen in het geval van buiten Nederland gevestigde dochters sprake is van een positief significant rendement [less ▲]

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See detailMutational analysis of the p50 subunit of NF-kappa B and inhibition of NF-kappa B activity by trans-dominant p50 mutants.
Bressler, P.; Brown, K.; Timmer, W. et al

in Journal of Virology (1993), 67(1), 288-93

The NF-kappa B family of DNA-binding proteins regulates the expression of many cellular and viral genes. Each of these proteins has an N-terminal region that is homologous to the c-Rel proto-oncogene ... [more ▼]

The NF-kappa B family of DNA-binding proteins regulates the expression of many cellular and viral genes. Each of these proteins has an N-terminal region that is homologous to the c-Rel proto-oncogene product, and this Rel homology region defines both DNA binding and protein dimerization properties of the individual proteins. Most of the NF-kappa B family members have been shown to associate with themselves or with each other to form homodimers or heterodimers, and previous studies have shown that dimerization of NF-kappa B factors is necessary to provide a functional DNA binding domain. We have used site-directed mutagenesis to identify regions in the Rel homology domain of the p50/NF-kappa B protein that are important for DNA binding and protein dimerization. Our studies have identified mutations of p50 that interfere with DNA binding only and those that interfere with protein dimerization. Mutations of p50 which disrupt only DNA binding were still able to associate with other members of the NF-kappa B protein family. We demonstrate that such heterodimeric complexes inhibit transcriptional activation mediated in trans through a cis-acting kappa B motif; therefore, we have identified trans-dominant negative mutants of p50. [less ▲]

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See detailLes cellules ECL et leurs récepteurs: rôle physiologique et intérêt physiopathologique
Belaiche, Jacques ULg; Delwaide, Jean ULg; Louis, Edouard ULg et al

in Acta Gastro-Enterologica Belgica (1993), 56(3-4, May-Aug), 235-44

The enterochromaffin-like (ECL) cells represent the predominating endocrine cell population in the oxyntic mucosa of the stomach. They are under the influence of gastrin. Recently, a histamine production ... [more ▼]

The enterochromaffin-like (ECL) cells represent the predominating endocrine cell population in the oxyntic mucosa of the stomach. They are under the influence of gastrin. Recently, a histamine production was shown within the secretory granules of ECL cells. ECL cells appear to play a crucial role in the physiology of gastric acid secretion. There are many unknowns concerning the intervention of other trophic factors in addition to gastrin and concerning the receptors located on the cells. ECL cell hyperplasia is a well documented consequence of hypergastrinemia. The latter can result from a gastrinoma or from a reduction of gastric acid secretion related to pernicious anemia or to long-term treatment with antisecretory drugs. [less ▲]

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See detailRole of Neuropeptides in Acetylcholine-Induced Edema in Isolated and Perfused Rabbit Lungs
Delaunois, Annie ULg; Gustin, Pascal ULg; Ansay, Michel ULg

in Journal of Pharmacology and Experimental Therapeutics (The) (1993), 266(2), 483-491

Changes in pulmonary endothelial permeability and in microvascular hemodynamics in response to cumulative concentrations of acetylcholine (ACh) (10(-8) M to 10(-5) M) were investigated in isolated ... [more ▼]

Changes in pulmonary endothelial permeability and in microvascular hemodynamics in response to cumulative concentrations of acetylcholine (ACh) (10(-8) M to 10(-5) M) were investigated in isolated, perfused rabbit lungs. The total pressure gradient was partitioned into four components: arterial, pre- and postcapillary and venous. The capillary filtration coefficient (Kf, c) also was evaluated. ACh caused a significant increase in arterial and precapillary pressures at concentrations higher than 3 x 10(-6) M. The total pressure gradient and precapillary were significantly increased whereas arterial, postcapillary and venous pressure gradient remained unchanged. In papaverine (3 x 10(-4) M)-pretreated lungs, the vasoconstriction was abolished and a concentration-dependent increase in Kf,c was recorded from 10(-8) to 10(-5) M ACh. This reaction was accompanied by pulmonary edema. Atropine, indomethacin, aspirin, ketanserin, clonidine, morphine and (+/-)-CP 96-345, an antagonist of neurokinin NK1 receptors, completely prevented the effects of ACh on Kf,c. In contrast, cromolyn sodium and SR48968, a neurokinin NK2 antagonist, did not inhibit the response to ACh. Terfenadine together with cimetidine had a partially inhibitory effect. Changes in the Kf, c similar to those observed with ACh were induced by capsaicin (10(-4) M) by exogenous substance P (10(-7) M) and by 5-hydroxytryptamine (5-HT) (10(-4) M). The effects of SP were inhibited by aspirin, (+/-)-CP 96,345 and ketanserin, but not by atropine and antihistaminics. 5-HT effects were prevented by aspirin and not by (+/-)-CP 96,345. It was concluded that ACh-induced pulmonary edema was due to an increase in the capillary filtration coefficient.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailRestriction Maps of the DNA of Cervid Herpesvirus 1 and Cervid Herpesvirus 2, Two Viruses Related to Bovine Herpesvirus 1
Vanderplasschen, Alain ULg; Bublot, M.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 128(3-4), 379-88

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as ... [more ▼]

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. [less ▲]

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See detailDémocratie, discussion et écriture chez Habermas et Lyotard
Delruelle, Edouard ULg

in Cahiers Internationaux de Symbolisme (1993), 74-76

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See detailON THE FORMATION OF PHOTOSYSTEM-II CHLOROPHYLL PROTEINS AFTER A SHORT LIGHT FLASH IN ETIOLATED BARLEY LEAVES, AS MONITORED BY INVIVO FLUORESCENCE SPECTROSCOPY
Franck, Fabrice ULg

in Journal of Photochemistry and Photobiology B : Biology (1993), 18(1), 35-40

The biogenesis of photosystem II (PSII) was investigated using 77 K fluorescence spectroscopy in etiolated barley leaves subjected to a millisecond flash which reduced all protochlorophyllide (Pchlide ... [more ▼]

The biogenesis of photosystem II (PSII) was investigated using 77 K fluorescence spectroscopy in etiolated barley leaves subjected to a millisecond flash which reduced all protochlorophyllide (Pchlide) into chlorophyllide (Chlide). In darkness after the flash, a slow chlorophyll (Chl) band shift from 682 to 684 nm was associated with the development of PSII variable fluorescence. Two Chl fluorescence bands at 678 and 687 nm were formed during the shift, the 687 nm band corresponding to the spectrum of the PSII variable fluorescence. The development of PSII fluorescence was inhibited by chloramphenicol and enhanced by cyclobeximide, suggesting a competition between plastid- and nucleus-encoded proteins for Chl binding. [less ▲]

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See detailEchec des élèves, échec de l'école ?
Crahay, Marcel ULg

in Recherche en Education. Théorie & Pratique (1993), 11-12

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See detailApplications de l'échographie en reproduction bovine. 2. L'utérus gestant et non-gestant.
Hanzen, Christian ULg; Laurent, Yves; Jakovljevic, Samy

in Annales de Médecine Vétérinaire (1993)

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See detailPotentialités de modulation de la réaction inflammatoire
Delannoy, I.; Miossec, P.; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (1993), 137

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See detailDe lugebere droom van W.F. Hermans. Hermans'mythologie in het licht van A. Schopenhauer
Yans, Baudoin ULg

in Spiegel der Letteren (1993), (1), 47-61

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See detailInventaire des végétaux éodévoniens de Belgique
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1993), 116

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See detailImprovement Yarrowia lipolytica lipase production by mutant selection.
Destain, Jacqueline ULg; Roblain, D.; Thonart, Philippe ULg

in Archives Internationales de Physiologie et de Biochimie (1993), 101(B1-B25),

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See detailEffect of prolactin on alpha and beta chloride cells in the gill epithelium of the saltwater adapted tilapia " Oreochromis niloticus"
Pisam, B.; Auperin, B.; Prunet, P. et al

in Anatomical Record (1993), 235

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the ... [more ▼]

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the latter, fishes were injected every 2 days for a week with tilapia prolactin (ti-PRL I). Gills were prepared for electron microscopy in order to determine the types and surface areas of chloride cells in each experimental condition. Two types of chloride cells, the alpha and beta cells were easily distinguished on the basis of their location and ultrastructural features in the gills of freshwater fishes, while only one type of cell, the saltwater alpha cells presumably derived from the transformation of the freshwater alpha cells, were encountered in saltwater adapted animals. After PRL injection of saltwater adapted fishes, small chloride cells, which displayed ultrastructural features similar to those of beta cells in freshwater tilapia, reappeared in interlamellar regions of the gills. In the same experimental conditions, the voluminous saltwater alpha cells showed a tendency to resume ultrastructural features more characteristic of the freshwater alpha cells from which they were derived. These observations tend to indicate that prolactin behaves as a "freshwater adapting hormone" and that beta cells are specifically involved in fish adaptation to freshwater living conditions. [less ▲]

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See detailRetrieval failures in face naming
Brédart, Serge ULg

in Memory (1993), 1(4), 351-366

Several authors have reported that the incidence of retrieval failures is higher for people's names than for object names. The first aim of the paper was to evaluate the role of one factor that might ... [more ▼]

Several authors have reported that the incidence of retrieval failures is higher for people's names than for object names. The first aim of the paper was to evaluate the role of one factor that might contribute to making face naming difficult. Face naming usually requires the retrieval of one specific label: the name of the seen individual. Object naming is less restricting. First, object names may have synonyms. Second, labels available from different levels of categorisation of an object may be appropriate to name that object (e.g. trousers, jeans, Levis). Such a degree of freedom does not exist in naming faces. The hypothesis that face naming is made difficult by the simple fact that people have only one name was tested by studying faces having the exceptional property of bearing two names: faces of actors playing nameable characters (e.g. Harrison Ford playing Indiana Jones). Consistent with the hypothesis, data from two experiments showed that when bypassing a block is possible by producing another name that is known for a face, the incidence of blocks falls dramatically. The other aim of the paper was to test the reversed frequency effect in person naming reported previously in several diary studies, in an experimental setting. A direct frequency effect rather than a reversed frequency effect was obtained in the present study. [less ▲]

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See detailLes aquifères crayeux en Belgique
Dassargues, Alain ULg; Monjoie, Albéric ULg

in Hydrogéologie (1993), (2), 135-145

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See detailSodium et hypertension artérielle essentielle. Nouveau refrain pour une vieille chanson
Krzesinski, Jean-Marie ULg

in L'Information Cardiologique (1993), 17(7), 299-304

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See detailQuantitative estimation of chimerism in mice using microsatellite markers
Delhaise, F.; Zhao, X.; Dessy, F. et al

in Molecular Reproduction and Development (1993), 34(2), 127-132

An embryonic stem cell line was established from SV129 mouse blastocysts and used to generate chimeric mice by injection into OF1 blastocysts; 18 out of the 30 resulting offspring appeared chimeric as ... [more ▼]

An embryonic stem cell line was established from SV129 mouse blastocysts and used to generate chimeric mice by injection into OF1 blastocysts; 18 out of the 30 resulting offspring appeared chimeric as judged from their coat color patterns, and 3 of the 13 males proved to be germ-line chimeras as they transmitted the SV129 agouti phenotype to all or part of their offspring. The degree of chimerism of these males was evaluated for different tissues using polymorphic microsatellite markers amplified by the polymerase chain reaction. It was shown that these new markers can be effectively used to quantitatively estimate levels of chimerism. The CKMM (creatine kinase, muscle) microsatellite system was used to distinguish the SV129 from the OF1 genotype. In all performed tests, the correlation between DNA ratio and signal ratio, expressed as a base 10 logarithm, was shown to exceed or equal 0.98 for known DNA ratios (SV129/OF1) ranging from 1/99 to 99/1. Linear calibration methods were used to predict the % SV129 DNA of a test sample based on the obtained signal ratio. The accuracy of the prediction was evaluated by performing repeated measurements. Differences among three repeated estimates ranged from 2 to 17% for a given sample. Microsatellite systems should be very useful to monitor chimerism involving strains that can not be discerned with coat color or biochemical markers. This will be particularly important when ES methodology becomes available in species other than mice. [less ▲]

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See detailMuscles rotateurs d'épaule et isocinétisme
Croisier, Jean-Louis ULg; Mommer, R.; Crielaard, Jean-Michel ULg

in Médecine et Hygiène (1993), 51

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See detailInfluence of body size on tibial nerve somato sensory evoked potentials in dogs
Poncelet, Luc; Michaux, Charles; Balligand, Marc ULg

in American Journal of Veterinary Research (1993)

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See detailRespiratory response to abdominal and rib cage muscle paralysis in dogs.
Brichant, Jean-François ULg; Gorini, Massimo; De Troyer, André

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1993), 74(5), 2309-17

To assess the respiratory response to abdominal and rib cage muscle paralysis, we measured tidal volume, esophageal and gastric pressures, arterial blood gases, and the electromyogram (EMG) of the ... [more ▼]

To assess the respiratory response to abdominal and rib cage muscle paralysis, we measured tidal volume, esophageal and gastric pressures, arterial blood gases, and the electromyogram (EMG) of the diaphragm during progressive epidural anesthesia (lidocaine 2%) in 35 supine anesthetized dogs. The EMG activity of the diaphragm was measured with fine-wire electrodes; the abdominal cavity was thus left intact. Paralysis of the abdominal muscles alone did not produce any alterations. In contrast, when all rib cage muscles were also paralyzed, there were substantial increases in the peak height and the rate of rise of diaphragmatic EMG activity that were associated with a decrease in tidal volume and an increase in arterial PCO2 (PaCO2); swings in transdiaphragmatic pressure, however, were unchanged. The increased diaphragmatic activation due to rib cage muscle paralysis persisted after bilateral cervical vagotomy and was well explained by the increased PaCO2. These observations indicate that in the dog 1) the rib cage muscles contribute significantly to tidal volume, and their paralysis causes, through the increased hypercapnic drive, a compensatory increase in diaphragmatic activation; and 2) the rib cage inspiratory muscles enhance the diaphragm's ability to generate pressure during breathing. [less ▲]

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See detailCytokine and anti-cytokine strategies in inflammatory reaction modulation
Delannoy, I.; Lekeux, Pierre ULg; Miossec, P.

in Veterinary Research (1993), 24

Evidence of the importance of a cytokine cascade in the induction and control of the inflammatory reaction is increasing. Although cytokines are required in the inflammatory process in response to ... [more ▼]

Evidence of the importance of a cytokine cascade in the induction and control of the inflammatory reaction is increasing. Although cytokines are required in the inflammatory process in response to infection or injury, their overproduction, particularly that of interleukin-1 (IL-1) and tumor necrosis factor (TNF), can lead to local/systemic pathology. Selective inhibition of the synthesis or of the action of specific cytokines may be of therapeutic benefit. Various strategies for blocking IL-1 and TNF activities have been presented. While selective inhibition of cytokine synthesis is still in the early experimental phase, specific blockade of soluble cytokine action following synthesis and release from cells is undergoing preliminary clinical trials in humans. Animal data suggest that antimonoclonal therapy such as monoclonal antibodies to TNF, IL-1 receptor antagonists or soluble receptors to TNF and IL-1 can be effective in the modulation of the inflammatory reaction. Modulation of the cytokine network in some diseases might also include the use of anti-inflammatory cytokines. Nonetheless, the possibilities of side effects due to impaired host-defense mechanisms with the IL-1 or TNF blockade must also be taken into consideration. [less ▲]

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See detailStereoselective hydrogenation of methacycline to doxycycline catalysed by rhodium-carborane complexes
Pirotte, Bernard ULg; Felekidis, Apostolos ULg; Fontaine, M. et al

in Tetrahedron Letters (1993), 34

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See detailonce a day administration of amikacine in neonates: assessment of nephrotoxicity and ototoxicity
Battisti, Oreste ULg; Langhendries, J. P.; Bertrand, J. M. et al

in Developmental Pharmacology and Therapeutics (1993), 20

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See detailMonads and chaos : the vitality of Leibniz's philosophy
Bouquiaux, Laurence ULg

in Diogenes (1993)

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See detailThiamine Triphosphate Activates an Anion Channel of Large Unit Conductance in Neuroblastoma Cells
Bettendorff, Lucien ULg; Kolb, Hans-Albert; Schoffeniels, E.

in Journal of Membrane Biology (1993), 136(3), 281-8

In neuroblastoma cells, the intracellular thiamine triphosphate (TTP) concentration was found to be about 0.5 microM, which is several times above the amount of cultured neurons or glial cells. In inside ... [more ▼]

In neuroblastoma cells, the intracellular thiamine triphosphate (TTP) concentration was found to be about 0.5 microM, which is several times above the amount of cultured neurons or glial cells. In inside-out patches, addition of TTP (1 or 10 microM) to the bath activated an anion channel of large unit conductance (350-400 pS) in symmetrical 150 mM NaCl solution. The activation occurred after a delay of about 4 min and was not reversed when TTP was washed out. A possible explanation is that the channel has been irreversibly phosphorylated by TTP. The channel open probability (Po) shows a bell-shaped behavior as a function of pipette potential (Vp). Po is maximal for -25 mV < Vp < 10 mV and steeply decreases outside this potential range. From reversal potentials, permeability ratios of PCl/PNa = 20 and PCl/Pgluconate = 3 were estimated. ATP (5 mM) at the cytoplasmic side of the channel decreased the mean single channel conductance by about 50%, but thiamine derivatives did not affect unit conductance; 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (0.1 mM) increased the flickering of the channel between the open and closed state, finally leading to its closure. Addition of oxythiamine (1 mM), a thiamine antimetabolite, to the pipette filling solution potentiates the time-dependent inactivation of the channel at Vp = -20 mV but had the opposite effect at +30 mV. This finding corresponds to a shift of Po towards more negative resting membrane potentials. These observations agree with our previous results showing a modulation of chloride permeability by thiamine derivatives in membrane vesicles from rat brain. [less ▲]

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See detailMiddle Devonian to Lower Carboniferous Miospores from selected boreholes in Amazonas and Parnaiba basins (Brazil): additional data, synthesis, and correlation.
Loboziak, S; Streel, Maurice ULg; Caputo, MV et al

in Documents du laboratoire de Géologie de Lyon (1993), 125

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See detailRed Blood Cell Na-Li Countertransport, Hypertensive Heredity, and Cardiovascular Risk in Young Adults
Krzesinski, Jean-Marie ULg; Saint-Remy, Annie ULg; Du, F. et al

in American Journal of Hypertension : Journal of the American Society of Hypertension (1993), 6(4), 314-316

The erythrocyte Na-Li countertransport (CT) has been considered as a marker of genetic propension to essential hypertension, but also to be linked to atherosclerosis risk factors. We have evaluated these ... [more ▼]

The erythrocyte Na-Li countertransport (CT) has been considered as a marker of genetic propension to essential hypertension, but also to be linked to atherosclerosis risk factors. We have evaluated these relationships by measuring the Na-Li CT, blood pressure levels, the family predisposition to hypertension, body mass index, subscapular skin-fold thickness, waist/hip ratio, and plasma lipids in 43 young adults (22 to 23 years; 13 with a positive family history of hypertension), followed since adolescence (+/- 10 years) to analyze the natural history of blood pressure in this period of life. The Na-Li CT was negatively correlated with the HDL cholesterol (r = -0.37) and the HDL cholesterol/total cholesterol ratio (r = -0.44). This transport system was positively correlated to family history of hypertension (r = 0.38), waist/hip ratio, and the diastolic blood pressure. When the family history of hypertension was present, Na-Li CT and diastolic blood pressure were higher (P < .05), but the HDL cholesterol was lower (P < .01). After separating people according to the blood pressure level during adolescence in two groups, one lower than the 75th percentile (P75), and one higher, we notice that the latter remains characterized by a higher systolic blood pressure. But neither the Na-Li CT nor family history of hypertension and plasma lipids could explain the difference in the blood pressure behavior during this period. Thus, an increase of RBC Na-Li CT activity in young adults would suggest a higher cardiovascular risk rather than to be a simple marker of a hypertensive risk. [less ▲]

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See detailMaghrébins en Europe
Martiniello, Marco ULg; Geisser, V.; Lorcerie, F. et al

in Annuaire de l'Afrique du Nord (1993), XXXII

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See detailLes auteurs latins et les langues étrangères
Rochette, Bruno ULg

in Latomus : Revue d'Etudes Latines (1993), 52

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See detailLa ferritine érythrocytaire
Guillemin, Ch; Revenant, M. C.; Vernet, V. et al

in Annales de Biologie Clinique (1993), 51

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See detailBiological control of postharvest Botrytis cinerea and Penicillium on apples
Jijakli, Haissam ULg; Lepoivre, Philippe ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (1993), (16), 106-110

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See detailFructose utilization during exercise in men: rapid conversion of ingested fructose to circulating glucose.
Jandrain, Bernard ULg; Pallikarakis, N.; Normand, S. et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1993), 74(5), 2146-54

The aim of the present study was to compare the metabolic fate of repeated doses of fructose or glucose ingested every 30 min during long-duration moderate-intensity exercise in men. Healthy volunteers ... [more ▼]

The aim of the present study was to compare the metabolic fate of repeated doses of fructose or glucose ingested every 30 min during long-duration moderate-intensity exercise in men. Healthy volunteers exercised for 3 h on a treadmill at 45% of their maximal oxygen consumption rate. "Naturally labeled" [13C]glucose or [13C]fructose was given orally at 25-g doses every 30 min (total feeding: 150 g; n = 6 in each group). Substrate utilization was evaluated by indirect calorimetry, and exogenous sugar oxidation was measured by isotope ratio mass spectrometry on expired CO2. Results were corrected for baseline drift in 13C/12C ratio in expired air due to exercise alone. Fructose conversion to plasma glucose was measured combining gas chromatography and isotope ratio mass spectrometry. Most of the ingested glucose was oxidized: 81 +/- 4 vs. 57 +/- 2 g/3 h for fructose (2P < 0.005). Exogenous glucose covered 20.8 +/- 1.4% of the total energy need (+/- 6.7 MJ) compared with 14.0 +/- 0.6% for fructose (2P < 0.005). The contribution of total carbohydrates was significantly higher and that of lipids significantly lower with glucose than with fructose. The blood glucose response was similar in both protocols. From 90 to 180 min, 55-60% of circulating glucose was derived from ingested fructose. In conclusion, when ingested repeatedly during moderate-intensity prolonged exercise, fructose is metabolically less available than glucose, despite a high rate of conversion to circulating glucose. [less ▲]

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See detailL'âge est-il un facteur limitant pour la chirurgie carotidienne?
Van Damme, Hendrik ULg; Smitz, Simon ULg; Albert, Adelin ULg et al

in Journal des Maladies Vasculaires (1993), 18(3), 245-53

To evaluate the role of carotid surgery in elderly patients 75 years and older, we reviewed 912 carotid endarterectomies performed on 806 patients between 1987 and 1990. There were 151 patients (19% of ... [more ▼]

To evaluate the role of carotid surgery in elderly patients 75 years and older, we reviewed 912 carotid endarterectomies performed on 806 patients between 1987 and 1990. There were 151 patients (19% of the whole series) aged 75 years and older (160 endarterectomies, group A), including 29 octogenarians, vs 655 patients under 75 years of age (group B). There were more women in the elderly age group (44%) than in the younger one (38%). Symptoms, risk factors, operative outcome and follow up data of the two groups were compared. The risk profile was similar for the two age groups, with exception for coronary heart disease, less frequent in the older patient group (25% had previous infarction vs 44%). Indication for carotid endarterectomy was different in the two age groups: 41% of group A underwent prophylactic thrombendarterectomy for high degree stenosis, while only 30% of group B had asymptomatic carotid disease. In group A, 6% of the patients had carotid endarterectomy after recovering from a mild stroke, vs 2% in group B. Angiography revealed bilateral carotid disease in 59% of the group A patients (including 15% with contralateral occlusion) vs 40% in group B. Operative mortality was 1.5% for the younger age group vs 2.5% for the older age group. The cause of death was cardiac in 60%. A follow up is available for all patients who benefited carotid endarterectomy since 1976, including 180 patients aged 75 years or older.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLinks between Egyptian and Greek Medicine
Marganne, Marie-Hélène ULg

in Forum (1993), 3(5), 35-43

Given the present state of our knowledge, the question about the links between Egyptian and Greek medicine is far from being resolved. Some convergences can in fact be detected, but when you study them ... [more ▼]

Given the present state of our knowledge, the question about the links between Egyptian and Greek medicine is far from being resolved. Some convergences can in fact be detected, but when you study them further, you will find that they reveal a loan, or a simple coincidence, or else a false interpretation of the sources. At the end of the sixth century BC, Pharaonic medicine was outclassed and influenced little by little by Greek medicine. Lastly, the Coptic medical treatises, composed in Egyptian language, but written in Greek characters, combine some Greek and finally Arabic borrowings. [less ▲]

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See detailDensitometric Determination of Salicin in Willow Stem Bark
Poukens-Renwart, Pascale; Tits, Monique ULg; Angenot, Luc ULg

in Journal of Planar Chromatography (1993), 6(november-december), 434-437

Most of the salicin present in willow stem bark occurs as labile glycosides (e.g. salicin, salicortin, 2-O'-acetylsalicortin, 3-O'-acetylsalicortin, tremulacin and fragilin). On alkaline hydrolysis, these ... [more ▼]

Most of the salicin present in willow stem bark occurs as labile glycosides (e.g. salicin, salicortin, 2-O'-acetylsalicortin, 3-O'-acetylsalicortin, tremulacin and fragilin). On alkaline hydrolysis, these acylated derivatives decompose to salicin; in this way, the total amount of salicin can be determined. A HPTLC method has been developed for the quantitative determination of salicin in stem bark before and after alkaline hydrolysis. The mobile phase used for the separation is a modification of one described in the literature. a double or triple migration is necessary to separate salicin from other components of the extract. The results have been compared with those obtained after bidimensional chromatography using two phases described for the quantitative evaluation of salicin. to check that separation was complete, the spectrum of salicin obtained from willow extracts was superimposed on that of a salicin standard.Quantification was performed at lambda = 270nm, the wavelenght of maximum absorption in the IV spectrum of salicin. [less ▲]

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See detailEffects of a 1-year treatment with a low-dose combined oral contraceptive containing ethinyl estradiol and cyproterone acetate on glucose and insulin metabolism.
Scheen, André ULg; Jandrain, Bernard ULg; Humblet, Dominique ULg et al

in Fertility and Sterility (1993), 59(4), 797-802

OBJECTIVE: To study the effects of the slightly estrogen-dominant monophasic low-dose oral contraceptive (OC) Diane-35 (Schering AG, Berlin, Germany) (35 micrograms ethinyl estradiol [EE2] + 2 mg ... [more ▼]

OBJECTIVE: To study the effects of the slightly estrogen-dominant monophasic low-dose oral contraceptive (OC) Diane-35 (Schering AG, Berlin, Germany) (35 micrograms ethinyl estradiol [EE2] + 2 mg cyproterone acetate, a 17 alpha-hydroxyprogesterone derivative [17-OHP]) on glucose and insulin metabolism. DESIGN: Seven healthy young women were investigated by using the euglycemic hyperinsulinemic glucose clamp technique (insulin delivery rate = 100 mU/kg per hour for 120 minutes). This test was performed, after an overnight fast, during the last 7 days of a spontaneous cycle and within the last 5 days of pill intake during the sixth and twelfth cycle of a continuous treatment with Diane-35 in each subject. RESULTS: The three indexes measuring the insulin-induced glucose disposal during the clamp (glucose infusion rate, glucose metabolic clearance rate, and glucose infusion rate divided by plasma insulin plateau levels) were not significantly affected by Diane-35. In contrast, the metabolic clearance rate of the exogenous insulin infused during the clamp tended to be slightly increased with Diane-35 (significant after 6 but not after 12 cycles). CONCLUSION: These results suggest that a 1-year treatment with the OC Diane-35, which contains EE2 + a 17-OHP rather than a 19-nortestosterone derivative as the progestogen compound, does not significantly alter peripheral (presumably muscular) insulin sensitivity but slightly increases insulin (presumably hepatic) clearance. [less ▲]

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See detailDifferential location of nucleic acids within interchromatin granule clusters.
Thiry, Marc ULg

in European Journal of Cell Biology (1993), 62(2), 259-69

We have examined in great detail the distribution of nucleic acids within interchromatin granule clusters in different cell types by means of various immunocytochemical approaches. Using the in situ ... [more ▼]

We have examined in great detail the distribution of nucleic acids within interchromatin granule clusters in different cell types by means of various immunocytochemical approaches. Using the in situ polyadenylate nucleotidyl transferase-immunogold technique for RNA detection or anti-RNA antibodies, we decisively demonstrate the presence of appreciable amount of RNA in clusters of interchromatin granules of untreated cells. Neither the in situ terminal deoxynucleotidyl transferase-immunogold technique nor anti-DNA antibodies, nor the in situ nick-translation technique for DNA detection have revealed any DNA in the interchromatin granule clusters. However, dispersed chromatin sensitive to DNase I are found at the borders and in the close vicinity of interchromatin granule clusters. The results indicate that interchromatin granule clusters should not be nuclear structures directly involved in RNA transcription but rather in some other steps of RNA metabolism. [less ▲]

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See detailinfections par streptocoque B en période néonatale
Battisti, Oreste ULg; Vanclaire, J.; François, A. et al

in Archives Françaises de Pédiatrie (1993), 50

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See detailLate Famennian miospore assemblages from the Bergisch Gladbach - Paffrath Syncline, Rhenish Slate Mountains, Germany
Hartkopf-Fröder, C; Streel, Maurice ULg

in Annales de la Société Géologique de Belgique (1993), 116(2), 333-357

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See detailRELATIVE IMPORTANCE OF FACTORS CONTROLLING THE LEACHING AND UPTAKE OF INORGANIC-IONS IN THE CANOPY OF A SPRUCE FOREST
Hambuckers, Alain ULg; Remacle, Jean ULg

in Biogeochemistry (1993), 23(2), 99-117

Sequential sampling of precipitation under mature spruce trees and time-series analysis of the data were performed in order to assess, in natural conditions, the relative importance of different factors ... [more ▼]

Sequential sampling of precipitation under mature spruce trees and time-series analysis of the data were performed in order to assess, in natural conditions, the relative importance of different factors that could influence the leaching and uptake of inorganic ions in the canopy. Eleven rain events were analyzed in order to estimate how external factors, rain intensity, H+, and ionic concentration of the incident rain influence the ionic throughfall concentrations and the net throughfall fluxes. The results led to the conclusion that leaching or uptake mostly occur by diffusion. The influence of the open rain acidity was not conclusive; however, it was shown that the tested external factors only controlled a few percent of the variation of the data. By contrast, the autocorrelation of the data always explained a large portion of the variance. lt could result from the gradual changes in the course time of internal factors related to the exchange system including waxes, cuticles, apoplast and xylem sap. These constituents were known to control the exchange at the canopy surface and to be sensitive to the plant physiology and environmental conditions. [less ▲]

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See detailEchographic Characterization of Carotid Plaques
VAN DAMME, Hendrik ULg; TROTTEUR, Geneviève ULg; Vivario, M. et al

in Acta Chirurgica Belgica (1993), 93(5), 233-8

The histology of the endarterectomy specimen was compared with the preoperative echopattern of 154 plaques. Thirty-four ultrasound images were of poor quality. The 120 adequate echographies revealed 22 ... [more ▼]

The histology of the endarterectomy specimen was compared with the preoperative echopattern of 154 plaques. Thirty-four ultrasound images were of poor quality. The 120 adequate echographies revealed 22 dense hyperechogenic homogeneous plaques, 28 soft hypoechogenic homogeneous plaques, 53 inhomogeneous plaques, and 13 mixed plaques. According to the surface, lesions were subdivided as smooth (n = 63) or irregular (n = 57). Echography recognized fibrous plaques (dense homogeneous hyperechogeneous lesions), with a specificity of 87% and a sensitivity of 56%. Recent intraplaque haemorrhage is echographically apparent as a hypoechogenic area in 88% of cases, what corresponds to a specificity of 79% and a sensitivity of 75%. Echography proved to be more accurate than angiography in determining the presence or absence of ulceration. The ability to characterize the pathology of carotid atheroma by duplex scan make the clinicopathologic correlation of carotid plaques of practical importance. [less ▲]

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See detailEnhancement of bovine leukemia virus-induced syncytia formation by di- and tripeptides.
Voneche, V.; Callebaut, I.; Lambrecht, B. et al

in Virology (1993), 192(1),

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by ... [more ▼]

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by bovine leukemia virus (BLV). Peptides containing a hydrophobic contribution and/or a positive net charge strongly enhanced syncytia formation induced by BLV on CC81 indicator cells. The size of the multinucleated cells was strongly increased (up to 10-fold) in the presence of the enhancer peptides whereas no effect was observed on the indicator cells in the absence of BLV. The peptides thus amplified the fusion process initiated by BLV envelope glycoproteins. The effect was dose-dependent at concentrations ranging from 20 to 640 microM and did not result from an increased expression of BLV proteins. The peptides did not compete with anti-gp51 monoclonal antibodies for the recognition of eight well-defined epitopes of gp51. We consequently hypothesize that the enhancer peptides interact with the membrane of BLV-producing cells and/or indicator cells and propose a model based on molecular modeling. [less ▲]

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See detailEarly prediction of response to recombinant human erythropoietin in patients with the anemia of renal failure by serum transferrin receptor and fibrinogen.
Beguin, Yves ULg; Loo, Martine; R'Zik, Samir ULg et al

in Blood (1993), 82(7), 2010-6

Recombinant human erythropoietin (rHuEpo) has been shown to be effective in correcting the anemia of chronic renal failure, but the dose needed may be variable. The reason for this variation is not known ... [more ▼]

Recombinant human erythropoietin (rHuEpo) has been shown to be effective in correcting the anemia of chronic renal failure, but the dose needed may be variable. The reason for this variation is not known, but several factors could be involved, such as iron deficiency, inflammation, aluminum intoxication, hyperparathyroidism, blood losses, or marrow dysfunction. Treatment with rHuEpo was given intravenously thrice weekly after hemodialysis to 64 consecutive unselected patients with the anemia of chronic renal failure. The starting dose was 50 U/kg/dose, which was increased to 75 and 100 U/kg/dose if no response was observed after 1 and 2 months of treatment. After a minimum follow-up of 6 months, response was evaluated as early (hematocrit [Hct] > or = 30% before 3 months) or late (Hct > or = 30% after 3 months) response, or failure (target Hct not attained). We examined the value of various laboratory parameters (baseline values and early changes) as predictors of response to rHuEpo. The best prediction by pretreatment parameters only was obtained with baseline serum transferrin receptor (TfR) (< or > or = 3,500 ng/mL) and fibrinogen (< or > or = 4 g/L): 100% response rate when both parameters were low, versus only 29% when they were both high, and versus 67% when one was low and the other high. When the 2-week TfR increment was greater than 20%, the response rate was 96%. When TfR increment was less than 20%, the response rate was 100% when baseline TfR and fibrinogen were low, 12% when fibrinogen was elevated, and 62% when fibrinogen was low but baseline TfR high. The predictive value of baseline TfR and fibrinogen and of the 2-week increment of TfR was confirmed by life table analysis and stepwise discriminant analysis. Major reasons for failure or late response were identified and included subclinical inflammation, iron deficiency, functional iron deficiency, marrow disorders, hemolysis, bleeding, and low Epo dose. We conclude that response to rHuEpo can be predicted early by pretreatment fibrinogen and TfR, together with early changes of TfR levels. These prognostic factors illustrate the importance of the early erythropoietic response, subclinical inflammation, and functional iron deficiency. Early recognition of a low probability of response in a given patient could help identify and correct specific causes of treatment failure to hasten clinical improvement and avoid prolonged ineffective use of an expensive medication. [less ▲]

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See detailTests simples pour le diagnostic présumé des Escherichia coli isolées principalement chez les bovins et productrices des cytotoxines nécrosantes (CNF)
Pohl, P.; Oswald, E.; Van Robaeys, G. et al

in Annales de Médecine Vétérinaire (1993), 137

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See detailMicroencapsulation by Coacervation of Poly(lactide-co-glycolide)-II:Encapsulation of a Dispersed Aqueous Phase
Nihant, Nicole; Stassen, S; Grandfils, Christian ULg et al

in polymer International (1993), 32

This paper is related to the phase separation of different copolyesters of lactides and glycolide solutions induced by the addition of silicone oil in order to promote microencapsulation of proteins. This ... [more ▼]

This paper is related to the phase separation of different copolyesters of lactides and glycolide solutions induced by the addition of silicone oil in order to promote microencapsulation of proteins. This coating process can be divided in three successive steps: phase separation of the coating polymer; adsorption of the coacervate droplets around the drugs phase, and microcapsule solidification. This paper focuses on the physico-chemical analysis of the second step. The knowledge of the interfacial tensions between the three liquid phases allows understanding of the microscopic evolution of the phase separation medium. [less ▲]

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See detailSpelling en spellingverandering : een slingerbeweging in de tijd
Vromans, Joseph ULg

in Nederlands van Nu (1993), 41

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See detailLes colibacilloses dans l'espèce bovine
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (1993), 137

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