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See detailChest wall motion during epidural anesthesia in dogs.
Warner, David O; Brichant, Jean-François ULg; Ritman, Erik L et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1991), 70(2), 539-47

To determine the relative contribution of rib cage and abdominal muscles to expiratory muscle activity during quiet breathing, we used lumbar epidural anesthesia in six pentobarbital sodium-anesthetized ... [more ▼]

To determine the relative contribution of rib cage and abdominal muscles to expiratory muscle activity during quiet breathing, we used lumbar epidural anesthesia in six pentobarbital sodium-anesthetized dogs lying supine to paralyze the abdominal muscles while leaving rib cage muscle motor function substantially intact. A high-speed X-ray scanner (Dynamic Spatial Reconstructor) provided three-dimensional images of the thorax. The contribution of expiratory muscle activity to tidal breathing was assessed by a comparison of chest wall configuration during relaxed apnea with that at end expiration. We found that expiratory muscle activity was responsible for approximately half of the changes in thoracic volume during inspiration. Paralysis of the abdominal muscles had little effect on the pattern of breathing, including the contribution of expiratory muscle activity to tidal breathing, in most dogs. We conclude that, although there is consistent phasic expiratory electrical activity in both the rib cage and the abdominal muscles of pentobarbital-anesthetized dogs lying supine, the muscles of the rib cage are mechanically the most important expiratory muscles during quiet breathing. [less ▲]

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See detailInduction expérimentale de la pneumonie pasteurellique chez la chèvre : étude des modifications physiopathologiques
Bakima, M.; Kaeckenbeeck, A.; Meniai, K. et al

in Annales de Médecine Vétérinaire (1991), 135

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See detailAbcès thyroïdien a streptocoque pneumoniae associé à un goitre nodulaire toxique. A propos d'un cas
Panas, N.; Zgheib, A.; Desaive, Claude ULg et al

in Semaine des Hôpitaux de Paris (La) (1991), 34

An unusual case of acute suppurative thyroiditis in association with toxic nodular goiter is reported by the authors. Surgical therapy was necessary due to the severity of the local and systemic ... [more ▼]

An unusual case of acute suppurative thyroiditis in association with toxic nodular goiter is reported by the authors. Surgical therapy was necessary due to the severity of the local and systemic inflammation induced by this association. [less ▲]

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See detailEvolution des pressions en oxygène et en dioxyde de carbone et du pH du sang artériel pendant la croissance
Bakima, M.; Lomba, F.; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (1991), 135

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See detailPollution of P Cygni line profiles by Fe IV and Fe V photospheric absorption in the ultraviolet spectrum of O-type stars
Nemry, F.; Surdej, Jean ULg; Hernaiz, A.

in Astronomy and Astrophysics (1991), 247

It is reported that the main pollution in the spectral ranges 1270-1500 and 1500-1860 A is caused by photospheric absorption lines of FeV and FeIV, respectively. Making use of the first order moment W1 of ... [more ▼]

It is reported that the main pollution in the spectral ranges 1270-1500 and 1500-1860 A is caused by photospheric absorption lines of FeV and FeIV, respectively. Making use of the first order moment W1 of a P Cygni line profile, it is shown that the effect of this photospheric pollution may lead to systematic errors, exceeding typically 50 percent in the determination of mass loss rates. It is also emphasized that the importance to correct the observed P Cygni line profiles for this pollution when studying the dependence of the physical parameters, terminal velocity, etc. of the stellar winds upon the various classes and types of O stars. [less ▲]

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See detailMonoclonal antibodies to oxytocin: production and characterization
Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean ULg et al

in Journal of Neuroimmunology (1991), 31

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin ... [more ▼]

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found to be very specific for OT using three different techniques (EIA, RIA and ICC); it si directed to the C-terminal tripeptide. The other two probably recongize tyrosine-containing epitope(s) also shared by vasopressin and other related nonapeptides. [less ▲]

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See detailUne plaquette filigranée du XIIIe siècle découverte à Stavelot. Identification et conservation
Van den Bossche, Benoît ULg; Dewanckel, Gilberte; Paquet, Pierre ULg et al

in Bulletin de l'Institut Royal du Patrimoine Artistique (1991), 23

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See detailVector mesons and partial restoration of chiral symmetry
Mendez Galain, Ramon; Ripka, Georges; Jaminon, Martine ULg et al

in Europhysics Letters [=EPL] (1991), 14

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See detailEffets du blocage des recepteurs beta-adrenergiques sur l'hyperlactacidemie induite par des exercices d'intensites differentes.
Scheen, André ULg; Camus, G.; Fossion, Anny ULg

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1991), 99(4), 331-4

The effects of beta-adrenergic blockade on the exercise-induced hyperlactatemia (Lap) have been studied in 31 adult male subjects [age: 25 +/- 1 years; body weight: 69 +/- 1 kg; VO2max: 54 +/- 1 ml O2.kg ... [more ▼]

The effects of beta-adrenergic blockade on the exercise-induced hyperlactatemia (Lap) have been studied in 31 adult male subjects [age: 25 +/- 1 years; body weight: 69 +/- 1 kg; VO2max: 54 +/- 1 ml O2.kg-1.min-1 (mean values +/- SEM)] randomly divided in 3 groups. All exercises were performed on a 10% inclined treadmill. In group 1 (n = 11), the subjects were walking during 20 minutes at 5 km.h-1 (55.6 +/- 1.4% VO2max). In group 2 (n = 10), they were running during 9 minutes at 8 km.h-1 (79.4 + 1.5% VO2max). The subjects of the third group (n = 10) were submitted to a 4 minutes run at 9.5 km.h-1 92 +/- 1.6% VO2max). These exercises were performed 1 hour after ingestion of a placebo or a single dose of 40 mg propranolol, in a double-blind randomized order. Blood samples were drawn at regular time intervals from an antecubital vein. Exercise tachycardia was reduced by about 20% (P less than 0.001) by propranolol in each group. Lap was significantly reduced by 15% by propranolol (P less than 0.005) at the lowest exercise intensity (55.6% VO2max), remained unchanged at 79.4% VO2max and was significantly enhanced by 16% during the recovery period following the run at 92% VO2max. These results clearly showed that the effects of acute beta-adrenergic blockade on Lap depend on exercise intensity. [less ▲]

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See detailThe germinal centre: a monastery or a bar?
Heinen, Ernst ULg; Tsunoda, T.; Marcoty, C. et al

in Research in Immunology (1991), 142(3), 242-4

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See detailThe cytokines: a possible role in sepsis
Damas, Pierre ULg

in Current Opinion in Anaesthesiology (1991), 4(2), 241-246

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See detailUne soeur aînée de la SNT: la ligue du Coin de Terre et du foyer insaisissable
Mougenot, Catherine ULg

in Cahiers de l'Urbanisme (Les) (1991), 9(Hiver), 56-68

Le mode de vie créé et véhiculé par le concept de "cité-jardin" s'est particulièrement bien imposé en Belgique. Explication sociologique et analyse des institutions constitutives de cette tendance.

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See detailLa recherche en Brucellose entreprise par le CEB (1e partie)
Limet, J.; Oanh Vo, T. K.; Saegerman, Claude ULg et al

in Les élevages belges (1991)

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See detailontogeny of radial and other astroglial cells in murine cerebral cortex
Misson, Jean-Paul ULg; takahashi, takao; caviness, verne S

in Glia (1991), 4

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See detailDes zones industrielles aux parcs scientifiques, technologiques et d'affaires. Trente ans de mutations des sites d'entreprises
Merenne-Schoumaker, Bernadette ULg

in Hommes et Terres du Nord (1991), 1991(2-3), 152-157

Thirty years of enterprise sites' mutations This article's object tries to study the enterprise sites' evolution since thirty years. With this aim in view, it confronts the sixty years sites (the ... [more ▼]

Thirty years of enterprise sites' mutations This article's object tries to study the enterprise sites' evolution since thirty years. With this aim in view, it confronts the sixty years sites (the industrial estates) and the to-day sites (the science, technological and business parks). It also analyses the impact of this evolution on the locations. The collected data show the growing importance of zonings and the weight of the enterprises' real estate. So locations of economic activities (new or transfered) are more and more influenced by the property developers' offer strategy (public and private sectors). If the first result of this evolution is to increase the spatial disparities (on regional and local levels), such an evolution also questions a certain classic literature where the demand's strategy explained the locations. [less ▲]

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See detailCo-operative binding of the glucocorticoid receptor DNA binding domain is one of at least two mechanisms for synergism.
Baniahmad, Claudia; Muller, Marc ULg; Altschmied, Joachim et al

in Journal of Molecular Biology (1991), 222(2), 155-65

Steroid induction of responsive genes functions through the synergistic activity of steroid receptor binding sequences with adjacent binding sites either for other transcription factors or for further ... [more ▼]

Steroid induction of responsive genes functions through the synergistic activity of steroid receptor binding sequences with adjacent binding sites either for other transcription factors or for further steroid receptors. Analysis of the human glucocorticoid receptor revealed that the DNA-binding domain of the receptor is sufficient to mediate co-operative binding to adjacent receptor binding sites. This is a novel feature of the domain in addition to its DNA-binding, trans-activating and trans-repressing properties. Chimaeric proteins containing the N- or C-terminal receptor halves fused to the GAL4 DNA-binding domain do not co-operate in DNA-binding, however they do functionally synergize. Thus, at least two mechanisms contribute to the synergism of the human glucocorticoid receptor bound to two adjacent receptor binding sites. [less ▲]

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See detailStudy of production of iturin from Bacillus subtilis : a potential antifungal agent.
Jacques, Ph; Vanhentenryck, F.; Thonart, Philippe ULg

in Archives Internationales de Physiologie et de Biochimie (1991), 99(B62),

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See detailHydrolysate preparation and comparative amino acid determination by cation-exchange and gas-liquid chromatography in diet ingredients
De Schrijver, Ronny; Van Eenaeme, Christian ULg; Fremaut, D. et al

in Cerevisia and Biotechnology (1991), 16(4)

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See detailA Note On The Rate Of Passage Of Digesta In Pigs Ileo-Rectostomized Or Fitted With An Ileal T-Cannula
Leterme, Pascal; Pirard, L.; Thewis, André ULg et al

in Animal Production (1991), 53(2), 253-256

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See detailHierarchy and scales in marine ecohydrodynamics
Nihoul, Jacques ULg; Djenidi, Salim ULg

in Earth-Science Reviews (1991), 31(3-4), 255-277

Recent investigations reveal, in marine ecosystems, an ecohydrodynamic hierarchical organization resulting from the different rates of ecological processes confronted to a multi-scale physical environment ... [more ▼]

Recent investigations reveal, in marine ecosystems, an ecohydrodynamic hierarchical organization resulting from the different rates of ecological processes confronted to a multi-scale physical environment. Major marine hydrodynamic processes are briefly analyzed here in an ecohydrodynamic perspective, emphasizing the effects they have on ecosystems at different levels of hierarchy and identifying appropriate “spectral windows” for modelling. A case study application to the Northern Bering Sea's Summer Ecohydrodynamics is given in illustration. [less ▲]

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See detailA method to compute reactive power margins with respect to voltage collapse
Van Cutsem, Thierry ULg

in IEEE Transactions on Power Systems (1991), 6

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See detailItaly : two Perspectives. Racism in Paradise ?
Martiniello, Marco ULg; Kazim, P.

in Race & Class (1991), 32(3), 79-89

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See detailLocating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy.
Thiry, Marc ULg; Thiry-Blaise, L.

in Nucleic Acids Research (1991), 19(1), 11-5

Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-external transcribed spacer ... [more ▼]

Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-external transcribed spacer sequence is predominantly visualized in the fibrillar centers of nucleoli, a non-transcribed spacer sequence is preferentially detected in the interstices, in close contact with the fibrillar centers and which interrupt the surrounding dense fibrillar component. Occasionally these two spacers are also observed in clumps of dense nucleolus-associated chromatin. These observations provide insights into the organization of ribosomal repeats within the nucleolus. [less ▲]

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See detailRight aortic arch with aberrant left subclavian artery. Report of two cases.
SAKALIHASAN, Natzi ULg; DEFRAIGNE, Jean ULg; Limet, Raymond ULg

in Surgical & Radiologic Anatomy [=SRA] (1991), 13(4), 327-331

Two cases of adult asymptomatic right aortic arch with an aberrant subclavian artery are reported. They were discovered at time of coronary surgery. Preoperative coronary arteriography failed to ... [more ▼]

Two cases of adult asymptomatic right aortic arch with an aberrant subclavian artery are reported. They were discovered at time of coronary surgery. Preoperative coronary arteriography failed to demonstrate the anomalies. In one case, the right arch was suspected on chest x-ray and preoperative barium oesophagography. In one case, the proximal suture of one saphenous bypass graft was performed on the left common carotid artery. Right aortic arch is a malformation rarely discovered in adults. It generally produces no symptoms when not associated with cardiac disease. [less ▲]

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See detailVariations saisonnières du régime alimentaire du chevreuil (Capreolus capreolus) en Haute Belgique
Degrez, Irma; Libois, Roland ULg

in Cahiers d'Ethologie (1991), 11(1), 17-30

In Belgium, information related to the deer food are quite uncommon and, as far as the roe deer is concerned, restricted to the hunting season (autumn). Our study was conducted in two areas of southern ... [more ▼]

In Belgium, information related to the deer food are quite uncommon and, as far as the roe deer is concerned, restricted to the hunting season (autumn). Our study was conducted in two areas of southern Belgium. The first, in Hotton (50°15 N, 5°27E) is a mixed oak-beech forest with a important cover of brambles. Faeces samples were collected monthly from October 1987 to September 1988. The second area, inside the Nature reserve of "Les Hautes Fagnes" (50°30N, 6°06 E; 600 m) is an old peat-bog (Eriophorum vaginatum, Molinia coerulea, Vaccinium myrtillus and V. uliginosum) surrounded by spruce plantations. Sampling took place there only in April and in June 1989. Faeces were boiled and filtered before digestion in the Scharrer- Kurschner mixture for 30 min. Then samples were sieved under water and a small amount taken and mounted on a slide for microscopic observation. More than 13,000 small vegetal pieces were identified to the species by comparison between the observed epidermal features and a reference collection of plant epidermis. Woody plants are the main food of the roe deer in both areas: in the Hautes Fagnes, heather (Calluna vulgaris) and bilberry (Vaccinium) are the main food items whereas in Hotton, brambles are the most important part the diet in each season. In Hotton, we found that the diet was much more diversified during the green season than in the winter. The winter diet comprises mainly brambles but also heather an Vaccinium myrtillus in low quantities. Spruce needles are present only at the end of the winter. In the spring, grasses were taken but their contribution to the food of the deer was never important. During the summer, the roe deer ate more tree leaves (Quercus robur and Sorbus aucuparia). In the Hautes Fagnes, grasses were important only in the spring diet and tree leaves (Sorbus) in the summer. Some tree species were very common in both study areas (beech and spruce saplings) but nearly not eaten. The roe deer can thus considered as a selective feeder preferring high quality foods (spring grasses or young leaves) to bulky one (old grasses or hard leaves). [less ▲]

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See detailPhotoionization Mass Spectrometry of Kinetic energy-selected ions. An Ion Retarding Potential Difference method applied to NO+ and O+ from N2O.
Locht, Robert ULg; Hagenow, G.; Hottmann, K. et al

in Chemical Physics (1991), 151

A new method is described to obtain photoionization efficiency curves of kinetic energy-selected fragment ions. The method is tested and discussed in the case of the dissociative photoionization yields of ... [more ▼]

A new method is described to obtain photoionization efficiency curves of kinetic energy-selected fragment ions. The method is tested and discussed in the case of the dissociative photoionization yields of NO+ and O+ from N2O. The attention is focussed on the dissociative autoionization processes in the threshold region, in the Franck-Condon region of the N2O(A2Sigma+) state and in the Franck-Condon gap between the A2Sigma+ and B2Pi states. [less ▲]

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See detailMICROSCOPIC RHEOLOGICAL MODELS AND EXTENDED IRREVERSIBLE THERMODYNAMICS
Dauby, Pierre ULg; Lebon, Georgy ULg

in Applied Mathematics Letters (1991), 4(6), 39-43

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See detailArrest of MCF-7 cell migration by laminin in vitro: possible mechanisms.
Coopman, P.; Verhasselt, B.; Bracke, M. et al

in Clinical & Experimental Metastasis (1991), 9(5), 469-84

Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by ... [more ▼]

Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha 6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100 micrograms/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin. [less ▲]

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See detailGeographic structure and potential ecological factors in Belgium
Dufrêne, Marc ULg; Legendre, P.

in Journal of Biogeography (1991), 18(3), 257-266

The available potential ecological factors have been scored in the form of presence/absence in Universal Transverse Mercator (UTM) squares in Belgium. A correspondence analysis shows a strong underlying ... [more ▼]

The available potential ecological factors have been scored in the form of presence/absence in Universal Transverse Mercator (UTM) squares in Belgium. A correspondence analysis shows a strong underlying gradient in the data set which induces an extraordinary horseshoe effect. This gradient follows closely the altitude component. Applying the k-means clustering method on UTM squares produced geographically compact groups which are largely hierarchically nested. This indicates strong regional trends in the ecological data set. As homogeneous groups may also be artefacts created by the clustering algorithms on a continuous gradient, the relevance of the borders between homogeneous areas is tested. In general, k-means borders correspond to the main breaking lines between adjacent UTM squares. They can be referred to as natural borders. -Authors [less ▲]

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See detailModélisation des mouvements aérodynamiques générés par le chauffage en culture protégée.
Aubinet, Marc ULg; Deltour, Jules; Nijskens, J.

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(1), 39-58

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See detailAN APPROACH TO THE ASYMMETRIC-SYNTHESIS OF L-6-[F-18]FLUORODOPA VIA NCA NUCLEOPHILIC FLUORINATION
Lemaire, Christian ULg; Guillaume, Marcel; Cantineau, Robert et al

in Applied Radiation & Isotopes (1991), 42(7), 629-635

The NCA asymmetric synthesis of L-6-[F-18]fluorodopa starting from (1R,2R,5R)-[(+)-2-hydroxypinanyl-3-idene]glycine t-butyl ester as chiral agent has been developed. After F-18-fluorination of the two ... [more ▼]

The NCA asymmetric synthesis of L-6-[F-18]fluorodopa starting from (1R,2R,5R)-[(+)-2-hydroxypinanyl-3-idene]glycine t-butyl ester as chiral agent has been developed. After F-18-fluorination of the two commercially available aldehydes either 6-nitroveratraldehyde or 6-nitropiperonal, the required alkylating [F-18]fluorobenzyl bromide derivative can be easily prepared by treatment with NaBH4 followed by SOBr2. Alkylation of the Schiff base was carried out with the lithium salt of 2,2,6,6-tetramethylpiperidine as base in anhydrous THF at -78-degrees-C. Following hydrolysis of the protecting groups with hydroxylamine and Hl, the L-amino acid was obtained in 75% L form (ee 50%) with a 10% decay corrected (120 min) radiochemical yield. [less ▲]

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See detailEssai de discrimination du tissu urbain par segmentation d'image
Collette, Bénédicte; Nadasdi, Istvan; Donnay, Jean-Paul ULg

in Mappemonde (1991), (4), 31-35

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See detailDaily Seasonalities on the Brussels Spot Equity Market
Corhay, Albert ULg

in Cahiers Economiques de Bruxelles (1991), (132), 415-430

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See detailAcyltransferase activities of the high-molecular-mass essential penicillin-binding proteins
Adam, Maggy; Damblon, Christian ULg; Jamin, Marc et al

in Biochemical Journal (1991), 279(Part 2), 601-604

The high-molecular-mass penicillin-binding proteins (HMM-PBPs), present in the cytoplasmic membranes of all eubacteria, are involved in important physiological events such as cell elongation, septation or ... [more ▼]

The high-molecular-mass penicillin-binding proteins (HMM-PBPs), present in the cytoplasmic membranes of all eubacteria, are involved in important physiological events such as cell elongation, septation or shape determination. Up to now it has, however, been very difficult or impossible to study the catalytic properties of the HMM-PBPs in vitro. With simple substrates, we could demonstrate that several of these proteins could catalyse the hydrolysis of some thioesters or the transfer of their acyl moiety on the amino group of a suitable acceptor nucleophile. Many of the acyl-donor substrates were hippuric acid or benzoyl-D-alanine derivatives, and their spectroscopic properties enabled a direct monitoring of the enzymic reaction. In their presence, the binding of radioactive penicillin to the PBPs was also inhibited. [less ▲]

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See detailLa topoclimatologie, un outil au service de l'agronomie. Exemples pris en Belgique
Erpicum, Michel ULg

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(1)

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See detailWord interruption in self-repairing
Brédart, Serge ULg

in Journal of Psycholinguistic Research (1991), 20(2), 123-137

Levelt (1983) proposed that the reason why speakers often do not interrupt an erroneous word before self-repairing is a lack of trouble detection before the end of that word. However, this explanation ... [more ▼]

Levelt (1983) proposed that the reason why speakers often do not interrupt an erroneous word before self-repairing is a lack of trouble detection before the end of that word. However, this explanation does not apply to merely inappropriate words. According to Levelt, this latter kind of word is completed for pragmatic reasons. In the present paper, a new corpus containing 1225 repairs is analyzed. From Levelt's theoretical framework, it was predicted that for erroneous words the longer the reparandum the higher the amount of word interruptions. Another prediction was that this decrease across word length should be slighter for nonerroneous words than for erroneous ones. Both predictions were confirmed. Results were consistent with Levelt's hypothesis and especially with the idea that erroneous word completion is not a real exception to the main interruption rule in speech self-repairing. [less ▲]

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See detailDiversity of the Mechanisms of Resistance to Beta-Lactam Antibiotics
Frère, Jean-Marie ULg; Joris, Bernard ULg; Granier, B. et al

in Research in Microbiology (1991), 142(6, Jul-Aug), 705-10

The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and ... [more ▼]

The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and properties of the beta-lactamase(s) and the diffusion barrier that the outer-membrane of Gram-negative bacteria can represent. Those three factors can be modified by mutations or by the horizontal transfer of genes or portions of genes. [less ▲]

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See detailNucleotide sequence of the bovine cyclic-AMP responsive DNA binding protein (CREB2) cDNA.
Willems, Luc ULg; Kettmann, Richard ULg; Chen, G. et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1991), 1(6),

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See detailAider l'apprenant à detecter ses propres erreurs : qu'en aurait dit DE FINETTI ?
Leclercq, Dieudonné ULg

in Enjeux : Revue de Formation Continuée et de Didactique du Français (1991), 22

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See detailLe décor des bâtons percés
Noiret, Pierre ULg

in Préhistoire ariégeoise - Bulletin de la Société préhistorique Ariège-Pyrénées (1991), XLVI

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See detailThree-dimensional electron microscopy of ribosomal chromatin in two higher plants: a cytochemical, immunocytochemical, and in situ hybridization approach.
Motte, Patrick ULg; Loppes, Roland ULg; Menager, M. et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1991), 39(11), 1495-506

We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the ... [more ▼]

We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the spatial organization of plant intranucleolar DNA, since their nucleoli have been previously reconstructed in 3-D from serial ultra-thin sections. We used the osmium ammine-B complex (a specific DNA stain) on thick sections of Lowicryl-embedded root fragments. Immunocytochemical techniques using anti-DNA antibodies and rDNA/rDNA in situ hybridization were also applied on ultra-thin sections. We showed on tilted images that the OA-B stains DNA throughout the whole thickness of the section. In addition, very low quantities of cytoplasmic DNA were stained by this complex, which is now the best DNA stain used in electron microscopy. Within the nucleoli the DNA was localized in the fibrillar centers, where large clumps of dense chromatin were also visible. In the two plant species intranucleolar chromatin forms a complex network with strands partially linked to chromosomal nucleolar-organizing regions identified by in situ hybridization. This study describes for the first time the spatial arrangement of the intranucleolar chromatin in nucleoli of higher plants using high-resolution techniques. [less ▲]

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See detailFouilles paléolithiques en Belgique méridionale (été 1992)
Noiret, Pierre ULg; Straus, Lawrence; Otte, Marcel ULg

in Vie archéologique (1991), 37

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See detailFantaisie verbale chez quelques monologuistes francophones
Defays, Jean-Marc ULg

in Bulletin Francophone de Finlande (1991), (7), 75-85

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See detailObservations ultraviolettes des planètes géantes et de leurs satellites à l'aide du Télescope Spatial Hubble
Gérard, Jean-Claude ULg; Dols, Vincent

in Nouvelles de la Science et des Technologies (1991)

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See detailTestosterone Metabolism in the Avian Hypothalamus
Balthazart, Jacques ULg

in Journal of Steroid Biochemistry & Molecular Biology (1991), 40(4-6), 557-70

Many central actions of testosterone (T) require the transformation of T into several metabolites including 5 alpha-dihydrotestosterone (5 alpha-DHT) and estradiol (E2). In birds as in mammals, 5 alpha ... [more ▼]

Many central actions of testosterone (T) require the transformation of T into several metabolites including 5 alpha-dihydrotestosterone (5 alpha-DHT) and estradiol (E2). In birds as in mammals, 5 alpha-DHT and E2, alone or in combination, mimic most behavioral effects of T. The avian brain is, in addition, able to transform T into 5 beta-DHT, a metabolite which seems to be devoid of any behavioral or physiological effects, at least in the context of reproduction. By in vitro product-formation assays, we have analyzed the distribution, sex differences and regulation by steroids of the 3 main T metabolizing enzymes (aromatase, 5 alpha- and 5 beta-reductases) in the brain of the Japanese quail (Coturnix c. japonica) and the zebra finch (Taeniopygia guttata castanotis). In the hypothalamus of quail and finches, aromatase activity is higher in males than in females. It is also decreased by castration and increased by T. The activity of the 5 alpha-reductase is not sexually differentiated nor controlled by T. The 5 beta-reductase activity is often higher in females than in males but this difference disappears in gonadectomized birds and no clear effect of T can be observed at this level. The zebra finch brain also contains a number of steroid-sensitive telencephalic nuclei [e.g. hyperstriatum ventrale, pars caudale (HVc) and robustus archistriatalis (RA)] which play a key role in the control of vocalizations. These nuclei also contain T-metabolizing enzymes but the regulation of their activity is substantially different from what has been observed in the hypothalamus. Aromatase activity is for example higher in females than in males in HVc and RA and the enzyme in these nuclei is not affected by castration nor T treatment. In these nuclei, the 5 alpha-reductase activity is higher in males than in females and the reverse is true for the 5 beta-reductase. These sex differences in activity are not sensitive to gonadectomy and T treatment and might therefore be organized by neonatal steroids. We have been recently able to localize aromatase-immunoreactive (AR-ir) neurons by ICC in the brain of the quail and zebra finch. Positive cells are found in the preoptic area, ventromedial and tuberal hypothalamus. AR-ir material is found in the perikarya of cells and fills the entire cellular processes including axons. At the electron microscope level, immunoreactive material can clearly be observed in the synaptic boutons. This observation raises questions concerning the mode of action of estrogens produced by central aromatization of T. [less ▲]

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See detailDecision Trees and Transient Stability of Electric Power Systems
Wehenkel, Louis ULg; Pavella, Mania ULg

in Automatica (1991), 27(1), 115-134

An inductive inference method for the automatic building of decision trees is investigated. Among its various tasks, the splitting and the stop splitting criteria successively applied to the nodes of a ... [more ▼]

An inductive inference method for the automatic building of decision trees is investigated. Among its various tasks, the splitting and the stop splitting criteria successively applied to the nodes of a grown tree, are found to play a crucial role on its overall shape and performances. The application of this general method to transient stability is systematically explored. Parameters related to the stop splitting criterion, to the learning set and to the tree classes are thus considered, and their influence on the tree features is scrutinized. Evaluation criteria appropriate to assess accuracy are also compared. Various tradeoffs are further examined, such as complexity vs number of classes, or misclassification rate vs type of misclassification errors. Possible uses of the trees are also envisaged. Computational issues relating to the building and the use of trees are finally discussed. [less ▲]

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See detailEtrangetés dans le Carnaval de Wilson Harris
Pagnoulle, Christine ULg

in Palimpsestes (1991), 6

The article offers an exploration of the disconcerting strangeness at work in Wilson Harris' novels and the strategies called for when translating them, on the basis of a couple of pages drawn from the ... [more ▼]

The article offers an exploration of the disconcerting strangeness at work in Wilson Harris' novels and the strategies called for when translating them, on the basis of a couple of pages drawn from the novel aptly entitled Carnival. [less ▲]

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See detailCytokines et choc septique
Lamy, Maurice ULg; Damas, Pierre ULg

in Bulletin de l'Académie Royale de Médecine de Belgique (1991), 146(6-7), 270

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See detailSequence analysis of the ARG7 gene of Schizosaccharomyces pombe coding for argininosuccinate lyase. Expression of the gene in Saccharomyces cerevisiae.
Loppes, Roland ULg; Michels, R; Decroupette, I et al

in Current Genetics (1991), 19(4), 255-60

The complete nucleotide sequence of the ARG7 gene, coding for argininosuccinate lyase (EC 4.3.2.1), in the fission yeast (Schizosaccharomyces pombe) has been determined. It consists of an open reading ... [more ▼]

The complete nucleotide sequence of the ARG7 gene, coding for argininosuccinate lyase (EC 4.3.2.1), in the fission yeast (Schizosaccharomyces pombe) has been determined. It consists of an open reading frame of 461 codons. The deduced protein has a molecular weight of 51,200 Da. The gene is devoid of introns which is confirmed by the fact that it is expressed in Escherichia coli after spontaneous insertion of a bacterial sequence probably bearing a prokaryotic promoter. A perfect "TATA" box is found at -72 and the major transcription initiation site in Saccharomyces cerevisiae is located at -11 as shown by primer extension experiments. Comparison of the S. pombe lyase with related proteins from other organisms reveals an important degree of conservation except in the carboxyterminal part of the polypeptide. Additionally, a deletion removing 66 amino acids of the carboxy terminus yields an enzyme exhibiting some biological activity. A unique 1,500 b transcript was found in S. cerevisiae when the intact gene was present, but the deleted version of the gene gave rise to at least three transcripts of 1,800, 2,800 and 3,900 b. [less ▲]

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See detailModèles mathématiques en hydrogéologie et paramétrisation
Dassargues, Alain ULg

in Annales de la Société Géologique de Belgique (1991), 113(2), 217-229

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See detailSciences de l’Antiquité et informatique
Denooz, Joseph ULg

in Nouvelles de la Science et des Technologies (1991), 9(1), 35-41

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See detailTransient T and B cell activation after neonatal induction of tolerance to MHC class II and Mls alloantigens
Schurmans, Stéphane ULg; Brighouse, G.; Kramar, G. et al

in Journal of Immunology (1991), 146

The neonatal injection of semiallogeneic F1 spleen cells into newborn parental mice results in the induction of tolerance to the corresponding alloantigen (alloAg) and chimerism. In these F1 cell-injected ... [more ▼]

The neonatal injection of semiallogeneic F1 spleen cells into newborn parental mice results in the induction of tolerance to the corresponding alloantigen (alloAg) and chimerism. In these F1 cell-injected mice, we have previously observed that this state of specific tolerance is associated with the development of a transient lupus-like autoimmune syndrome. In this study, we show that neonatal injection of mice with spleen cells differing from the host at major histocompatibility complex (MHC) class I, class II, class (I + II), or minor lymphocyte stimulating (Mls) alloAg induced a state of specific tolerance characterized by the absence of alloreactive CTL and/or Th cell responses in the spleen and the thymus of 6- to 12-week-old injected mice. However, in mice rendered tolerant to MHC class II or class (I + II) alloAg, the presence of high levels of IgG1 antibodies, of circulating immune complexes, of anti-ssDNA autoantibodies, and of tissue lesions were transiently observed. In these mice, an increased Ia Ag expression on lymphoid spleen cells was also detected at 1 wk. The elevated production of IgG1 and the overexpression of Ia Ag were almost completely prevented by treatment with an anti-IL-4 mAb. Such manifestations of B cell activation and autoimmunity were not observed in mice neonatally injected with F1 cells differing from the host only at MHC class I Ag. In mice neonatally tolerized to Mls Ag, a transient increase in IgG2a production and an overexpression of Ia Ag were detected without features of autoimmunity, and were prevented by anti-INF-gamma mAb treatment. In mice rendered tolerant to MHC class II, class (I + II), or Mls alloAg at birth, the manifestations of B cell activation were associated with the presence of in vivo-activated alloreactive CD4+ T cells in the spleen--but not the thymus--of 1-wk-old injected mice. Together, these results suggest that in mice neonatally injected with semiallogeneic F1 cells, the process of tolerance induction is not efficient during the early postnatal period, and could allow the maturation and peripheralization of some alloreactive CD4+ T cells, leading to transient B cell activation and, depending on the alloAg, to autoimmunity [less ▲]

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See detailChemical anatomy of the spinal cord
Schoenen, Jean ULg

in Neurologic Clinics (1991), 9

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See detailDe Grady suffragant. Dialogue rimé et chanson publiés à Liège en 1762
Boutier, Marie-Guy ULg

in Dialectes de Wallonie (1991), 19/20

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See detailLeukemia Viruses
Burny, A.; Willems, Luc ULg

in Encyclopedia of Immunology, Academic Press (1991)

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See detailChoice of sedation according to ventilatory mode
Lamy, Maurice ULg; Damas, Pierre ULg; Joris, Jean ULg

in Journal of drug development (1991), 4(Suppl 3), 77-81

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See detailThe glucocorticoid receptor.
Muller, Marc ULg; Renkawitz, R.

in Biochimica et Biophysica Acta (1991), 1088

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See detailStructure de l'acide (tert-butylamino-4 oxo-5 triazol-1,2,4 yle-1)acétique
Dupont, L.; Englebert, S.; Dideberg, O. et al

in Acta Crystallographica (1991), C47

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See detailTruncal Vagotomy and Pyloroplasty Combined with Valvular Replacement in Patients with Ulcer Disease
Defraigne, Jean-Olivier ULg; Innocenti, C.; DEKOSTER, Guy ULg et al

in Journal of Cardiovascular Surgery (The) (1991), 32(1), 42-5

In 1988, 5 patients (3 men and 2 women) with ulcer disease (mean age 56 +/- 8 years) underwent valvular replacement for aortic (No. = 4) or mitral disease (No. = 1). All patients had had gastroduodenal ... [more ▼]

In 1988, 5 patients (3 men and 2 women) with ulcer disease (mean age 56 +/- 8 years) underwent valvular replacement for aortic (No. = 4) or mitral disease (No. = 1). All patients had had gastroduodenal ulcers. Preoperative gastroscopy demonstrated active ulcers (No. = 4) and a healed pyloric ulcer with pyloric stenosis (No. = 1). Despite the presence of ulcers, a non-biologic prosthesis was preferred in each patient because of their young age (No. = 3), chronic atrial fibrillation requiring anticoagulant therapy (No. = 1), and refusal of the eventuality of subsequent reoperation (No. = 1). In each patient, a truncal vagotomy with pyloroplasty was performed simultaneously with the valvular procedures by the same incision. The postoperative courses were uneventful. With a mean follow-up of 15 +/- 3 months, no gastrointestinal bleeding was observed during anticoagulant therapy. With anticoagulant drugs, bleeding may occur with a frequency of 4% per patient treatment-year, half of which are gastrointestinal in origin. Nevertheless, in selected patients with gastroduodenal ulcers, performing a vagotomy-pyloroplasty simultaneously with valvular replacement allows implantation of a non-biologic prosthesis, with greater durability than bioprosthesis. [less ▲]

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See detailCutaneous and basophilic sensitivity to substance P and gastrin in non-atopic versus atopic subjects.
Louis, Renaud ULg; Radermecker, Maurice ULg

in Allergy (1991), 46(1), 30-4

We compared the cutaneous reaction to intradermal injection of substance P, gastrin and histamine in asymptomatic atopic subjects with a history of hay fever and/or asthma versus non-atopic healthy ... [more ▼]

We compared the cutaneous reaction to intradermal injection of substance P, gastrin and histamine in asymptomatic atopic subjects with a history of hay fever and/or asthma versus non-atopic healthy volunteers. We also studied in these two groups the basophilic histamine release induced by substance P and gastrin with that obtained with anti-human IgE and Con A. Intradermal injection of substance P (3-300 pM) and gastrin (3-30 pM) caused a wheal and flare reaction which was comparable in both groups of subjects. Substance P 10(-4)M caused a mean basophilic histamine release of about 15% in atopic and non-atopic subjects. Gastrin was not effective in this model. Anti-IgE and Con A-induced histamine release was significantly higher in atopic than in non-atopic volunteers. [less ▲]

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See detailÉtude hydrodynamique d'un fermenteur airlift adapté pour le mode de fonctionnement fed-batch
Groslambert, Sylvie ULg; Schlitz, Marc; Crine, Michel ULg

in Récents Progrès en Génie des Procédés (1991), 5(16), 11-16

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See detailA Note On The Comparison Of Methods For Estimating The Ileal Digestibility Of Amino-Acids In Pigs
Leterme, Pascal; Pirard, L.; Thewis, André ULg

in Animal Production (1991), 52(1), 404-406

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See detailElectron microscopical investigations of hexagonal phase precipitation in Zn---12 wt% Al---1 wt% Cu and Zn---27 wt% Al---2 wt% Cu alloys
Rachev, P.; Terziev, L.; Lecomte-Beckers, Jacqueline ULg et al

in Acta Metallurgica et Materialia (1991), 39(9), 2177-2182

In Zn---12 wt% Al---1 wt% Cu and Zn---27 wt% Al---2 wt%Cu alloys aged at 100° and 250°C the precipitation of h.c.p. phases was studied by means of transmission electron microscopy. The presence of the ... [more ▼]

In Zn---12 wt% Al---1 wt% Cu and Zn---27 wt% Al---2 wt%Cu alloys aged at 100° and 250°C the precipitation of h.c.p. phases was studied by means of transmission electron microscopy. The presence of the metastable ηm and ηx-phases was established using the Moiré pattern method. The ηm → η phase transition was observed. Screw dislocations are visualized in (114) with b logical and {220} in η phase precipitates by means of Moiré fringes. [less ▲]

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See detailDoes environmental exposure to cadmium represent a health risk? Conclusions from the Cadmibel Study.
Lauwerys, R.; Bernard, A.; Buchet, J.-P. et al

in Acta Clinica Belgica (1991), 46

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See detailL'intérêt porté par les poètes et les traducteurs russes à l'Ode III, 30 d'Horace (Exegi monumentum)
Mouton, Charles ULg

in Latomus : Revue d'Etudes Latines (1991), L(3), 654-670

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See detailUne évolution inéluctable
Reginster, Jean-Yves ULg

in Jeune Pratique (1991), 50

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See detailBlunted erythropoietin production and decreased erythropoiesis in early pregnancy.
Beguin, Yves ULg; Lipscei, G.; Thoumsin, H. et al

in Blood (1991), 78(1), 89-93

After decreasing in the first trimester of pregnancy, the total red blood cell mass increases in the second and third trimesters to peak at term at about 120% to 125% of nonpregnant values, but how this ... [more ▼]

After decreasing in the first trimester of pregnancy, the total red blood cell mass increases in the second and third trimesters to peak at term at about 120% to 125% of nonpregnant values, but how this is brought about by changes in the rate of erythropoiesis is not known. We evaluated erythropoiesis by measuring serum transferrin receptor (TfR) levels in 406 women during normal pregnancy (N = 317), at delivery (N = 63), or in the early postpartum (N = 27). Despite the presence of the placenta and the frequent occurrence of iron deficiency, TfR levels remained low in the first two trimesters and increased in the third trimester and at delivery. To explain why erythropoiesic activity was relatively low in early pregnancy, we also measured serum immunoreactive erythropoietin (Epo) in relation to the degree of anemia. There was a very strong correlation between serum TfR and Epo levels in the entire group (r = .59, P less than .0001) as well as in each period of pregnancy. Epo levels remained low for the degree of anemia and did not correlate with hematocrit in the first two trimesters, but recovered afterwards. In the early postpartum, Epo production and erythropoiesis were normal. We conclude that: (1) erythropoiesis is decreased in the first part of pregnancy but increases afterwards; and (2) blunted Epo production in early pregnancy could be responsible for that observation. [less ▲]

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See detailLes vérocytotoxines d'Escherichia coli et leur diagnostic par sondes génétiques
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (1991), 135

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See detailVelogenetics or the synergistic use of Marker Assisted Selection and Germ-Line Manipulation
Georges, Michel ULg; Massey, J. M.

in Theriogenology (1991), 35(1), 151-156

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See detailNouvelles données sur la bande de Sambre-et-Meuse à Ombret (Huy, Belgique)
Hance, Luc ULg; Steemans, Philippe ULg; Goemaere, E et al

in Annales de la Société Géologique de Belgique (1991), 114(1), 253--264

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See detailObservations on seed protein electrophoretic patterns of some species within the genus Phaseolus.
Baudoin, Jean-Pierre ULg; Schmit, V.; Wathelet, Bernard ULg

in Annual Report of the Bean Improvment Cooperative (1991), 34

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See detailLes Pachytheca de La Gileppe et de Nonceveux (Dévonien inférieur de Belgique)
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1991), 113

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See detailIntroduction à l'arithmétique des algèbres de quaternions
Pays, Isabelle ULg; Tignol, Jean-Pierre

in Note di Matematica e Fisica, Cerfim, Locarno (5) (1991)

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See detailFaire partie d’une association, aujourd’hui : qui sont les jeunes qui participent, au Québec, en France et en Belgique ?
Fournier, Bernard ULg

in Égalité ; revue acadienne d’analyse politique (1991), 30(automne), 39-67

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See detailPour un roman sans manifeste
Demoulin, Laurent ULg

in Ecritures (1991), 1

Description des nouvelles tendances du roman écrit en français.

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See detailReproductive Development in Lolium temulentum L.: Spike Morphogenesis and Grain Set Limitations
Périlleux, Claire ULg; Bernier, Georges ULg; Kinet, Jean Marie

in Journal of Experimental Botany (1991), 42(4), 501

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was ... [more ▼]

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was to assess whether Lolium temulentum strain Ceres, a qualitative long-day grass, could serve as a model of reproductive development for cereals. The morphological patterns from floral transition to seed set were studied. [less ▲]

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See detailAdult respiratory distress syndrome: local or systemic disease
Deby, Ginette ULg; Lamy, Maurice ULg; Deby, C. et al

in Critical Care (1991), 4(1), 57-83

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See detailEffects of different calcium formulations on calcium absorption in healthy volunteers
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Franchimont, P

in Advances in Therapy (1991), 8(2), 86-91

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See detailSEARCH FOR PARVALBUMIN IN CARDIAC-MUSCLE OF NORMAL AND HEMOGLOBIN-MYOGLOBIN FREE ANTARCTIC FISH
Laforet, C.; Feller, Georges ULg; Gerday, Colette ULg

in Journal of Muscle Research and Cell Motility (1991), 12(1), 115-116

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See detailA Densitometric Method for the Determination of Three Clinically Important Monosaccharides in Urine
Poukens-Renwart, Pascale; Angenot, Luc ULg

in Journal of Planar Chromatography (1991), 4(January/February), 77-79

A quantitative, densitometric, thin layer chromatographic method has been developed for the determination of three clinically important monosaccharides in ureine, after desalting.The analysis of these ... [more ▼]

A quantitative, densitometric, thin layer chromatographic method has been developed for the determination of three clinically important monosaccharides in ureine, after desalting.The analysis of these monosaccharides in urine allows to observe the potential changes induced by antiinflammatories in human small intestine permeability. Xylose, 3-O-methylglucose and rhamnose were estimated quantitatively in urine; arabinose was used as internal standard. The densitometry was performed at a wavelenght of 400 nm after a visualization reaction on HPTLC plates. In all cases, coefficients of variation of the method were less than 2.7%. [less ▲]

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See detailStudy of the Mechanism of Siderophore Production by Transposon-Mutagenesis of Pseudomonas fluorescens
Delfosse, Ph; Jacques, Ph.; Cornelis, P. et al

in Archives Internationales de Physiologie et de Biochimie (1991), 99(B7),

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See detailLes télécommunications d'entreprise : influence sur le développement régional et la répar­tition spatiale des activités
Jacob, Damien ULg

in Bulletin de la Société Belge d'Etudes Géographiques = Tijdschrift van de Belgische Vereniging voor Aardrijkskundige Studies (1991)

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See detailSpectroscopic investigation of structure in octarellin (a de novo protein designed to adopt the alpha/beta-barrel packing)
Beauregard, Marc; Goraj, Karine; Goffin, Vincent et al

in Protein Engineering (1991), 4(7), 745-9

We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990) Protein ... [more ▼]

We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990) Protein Engng, 3, 259-266]. Infrared and Raman spectra analyses of octarellin's secondary structure reveal the expected percentage of alpha-helices (30%) and a higher beta-sheet content (40%) than predicted from the design. When the Raman spectra obtained with octarellin and native triosephosphate isomerase (a natural alpha/beta-barrel) are compared, similar percentages of secondary structures are found. Thermal denaturation of octarellin monitored by CD confirms that its secondary structures are quite stable, whereas its native-like tertiary fold is not. Tyrosine residues, predicted to be partially hidden from solvent, are actually exposed as revealed by Raman and UV absorption spectra. We conclude that the attempted alpha/beta-barrel conformation in octarellin may be loosely packed. The criteria used to design octarellin are discussed and improvements suggested. [less ▲]

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See detailEtude lithostratigraphique, biostratigraphique et sédimentologique du sondage de Lessines (bord méridional du Massif de Brabant, Belgique)
Herbosch, A.; Vanguestaine, M.; Degardin, J. M. et al

in Anales de la société géologique de belgique (1991), 114

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See detailAbnormal gene expression in skin fibroblasts from a Hutchinson-Gilford patient.
Colige, Alain ULg; Roujeau, J. C.; De la Rocque, F. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1991), 64(6), 799-806

We had the opportunity to investigate a new case of Hutchinson-Gilford progeria, a rare disease commonly regarded as a model in the study of aging. Two strains of fibroblasts (strains 1 and 2) were ... [more ▼]

We had the opportunity to investigate a new case of Hutchinson-Gilford progeria, a rare disease commonly regarded as a model in the study of aging. Two strains of fibroblasts (strains 1 and 2) were derived from two pieces of a skin biopsy. These two populations multiplied as normal cells at low population doubling level but senesced rapidly and stopped proliferating after 14 or 15 population doubling levels. Interestingly, an unusual pattern of growth in clusters was observed for strain 1. The level of collagen and noncollagen protein synthesis of both strains of affected fibroblasts was similar to that of normal fibroblasts as determined by [3H]proline incorporation measurement and was similarly affected by varying serum concentrations. The pattern of the main types of newly synthesized collagen polypeptides analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was similar in normal and progeria cells. The steady-state level of mRNAs coding for macromolecules of the extracellular matrix did not provide any differences between affected and control fibroblasts except for a strong increase of elastin and of alpha 1 and alpha 2 type IV procollagen mRNA mainly in strain 1 and less marked in strain 2. Interestingly, senescent progeria fibroblasts exhibited a reduced level of all the tested mRNAs, whereas collagen type IV and elastin mRNAs remained elevated. As suggested by immunofluorescence and immunoblotting studies, the increased amount of type IV mRNAs was paralleled by an enhanced production of type IV collagen by fibroblasts in vitro. Histologic examination of the skin revealed a superabundant network of abnormal elastic fibers in the reticular dermis and a thickening of basement membranes. The relationship between these alterations and aging in progeria is discussed. [less ▲]

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See detailTypicality in categorization, recognition and identification : evidence from face recognition
Valentine, Tim; Ferrara, André ULg

in British Journal of Psychology (1991), 82

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See detailThe synthesis of no-carrier-added DL-4-[18F]fluorodeprenyl via the nucleophilic aromatic substitution reaction.
Plenevaux, Alain ULg; Fowler, Joanna S.; Dewey, Stephen L. et al

in International Journal of Radiation Applications and Instrumentation. Part A : Applied Radiation and Isotopes (1991), 42(2), 121-7

No-carrier-added DL-alpha-methyl-beta-4-[18F]fluorophenyl-N-methyl-N-propynylethylamin e (DL-4-[18F]fluorodeprenyl) was synthesized via the following 3-step procedure: (1) nucleophilic aromatic ... [more ▼]

No-carrier-added DL-alpha-methyl-beta-4-[18F]fluorophenyl-N-methyl-N-propynylethylamin e (DL-4-[18F]fluorodeprenyl) was synthesized via the following 3-step procedure: (1) nucleophilic aromatic substitution by [18F]fluoride on 4-nitrobenzaldehyde to produce 4-[18F]fluorobenzaldehyde (yield 65%); (2) the reaction of 4-[18F]fluorobenzaldehyde with (1-chloro-1-(trimethylsilyl)ethyl)lithium followed by hydrolysis to give 4-[18F]fluorophenylacetone (yield 50%); and (3) reductive alkylation of 4-[18F]fluorophenylacetone with N-methyl-propynylamine in the presence of NaBH3CN (yield 35%) followed by HPLC purification to give a racemic mixture of 4-[18F]fluorodeprenyl. The overall yield was 11% (EOB corrected), the synthesis time was 90 min and the specific activity greater than 0.57 Ci/mumol (end of synthesis). This synthesis approach, the conversion of an aromatic aldehyde to a homologous methyl ketone, extends the flexibility of the nucleophilic aromatic substitution reaction by applying it to the synthesis of radiotracers which do not bear electron-withdrawing activating groups on the aromatic ring. The tissue distribution of DL-4-[18F]fluorodeprenyl in mice at 1, 10 and 60 min was also measured and showed that metabolic defluorination was not significant. Clearance of radioactivity from brain after injection of DL-4-[18F]fluorodeprenyl was more rapid than that previously observed for [11C]L-deprenyl. [less ▲]

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See detailTAXONOMIC STUDY OF MARINE OSCILLATORIACEAN STRAINS (CYANOPHYCEAE, CYANOBACTERIA) WITH NARROW TRICHOMES .1. MORPHOLOGICAL VARIABILITY AND AUTECOLOGICAL FEATURES
Wilmotte, Annick ULg

in Archiv für Hydrobiologie (1991), 92

Twenty marine and one freshwater strain, belonging to the genera Oscillatoria, Phormidium, Plectonema and Spirulina (sensu GEITLER), exhibiting a cell width less than 3-mu-m were cultivated in different ... [more ▼]

Twenty marine and one freshwater strain, belonging to the genera Oscillatoria, Phormidium, Plectonema and Spirulina (sensu GEITLER), exhibiting a cell width less than 3-mu-m were cultivated in different light intensities, temperatures and salinities. The study of the morphological variability allows one to determine the stable and variable morphological characters. The tolerances to the culture conditions, the possibility of survival in unfavourable conditions and the capacity of chromatic adaptation were investigated. The autecological characters are discussed in relation to the original habitat as well as to the taxonomic grouping of the strains based on the morphology. [less ▲]

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See detailEvidence of West European defined miospore zones in the Uppermost Devonian and Lower Carboniferous of the Amazonas Basin (Brazil).
Loboziak, S; Streel, Maurice ULg; Caputo, M.V. et al

in Geobios (1991), 24(1), 5-11

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See detailScale economies and performances in the French Insurance Industry
Fecher-Bourgeois, Fabienne ULg; Kessler, D.; Pestieau, Pierre ULg et al

in Geneva Papers on Risk & Insurance - Issues & Practice (1991), (60),

The purpose of this paper is to provide for both life and non life insurance a measurement of economies of scale and an assessment of productive performance of French companies. We use a translog cost ... [more ▼]

The purpose of this paper is to provide for both life and non life insurance a measurement of economies of scale and an assessment of productive performance of French companies. We use a translog cost function to check the presence of scale economies and a parametric approach to construct our yardstick of productive efficiency. Our panel data covers 84 life and 243 non life companies for the period 1984–89. The main findings indicate both the existence of increasing returns to scale and a wide dispersion in the rates of inefficiency across companies. Those two results are shown to be closely linked to a number of characteristics of the companies studied, particularly their institutional form. [less ▲]

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See detailLa surveillance de l'état de l'environnement wallon par bioindicateurs.
Goffart, Philippe; Dufrêne, Marc ULg; Marchal, Didier

in Gomphus (1991), 7

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See detailEnquete serologique sur la prevalence de l'infection par l'herpes virus bovin type 4 dans les troupeaux bovins des plaines d'Accra au Ghana.
Marchot, Philippe; Thiry, Etienne ULg; Jetteur, P. et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (1991), 44(4), 405-6

Sera from cattle of the Accra Plains in Ghana were screened for bovine type-4 herpesvirus (BHV-4) antibodies by an indirect immunofluorescence test. Among the 176 screened serums, 14% were found positive ... [more ▼]

Sera from cattle of the Accra Plains in Ghana were screened for bovine type-4 herpesvirus (BHV-4) antibodies by an indirect immunofluorescence test. Among the 176 screened serums, 14% were found positive at a 1/100 dilution. Further studies are necessary for a better understanding of the relationships between BHV-4 or some other agents and necrotic vaginitis associated with poor fertility observed in cattle in this area. [less ▲]

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See detailProduction and purification of biologically active recombinant tilapia (Oreochromis niloticus) prolactins
Swennen, D.; Rentier-Delrue, Françoise ULg; Auperin, B. et al

in Journal of Endocrinology (1991), 131(2), 219-27

Recombinant expression vectors carrying tilapia prolactin-I or -II (tiPRL-I or tiPRL-II) cDNA were constructed and the tiPRL-I and II proteins were produced in E. coli as inclusion bodies. These inclusion ... [more ▼]

Recombinant expression vectors carrying tilapia prolactin-I or -II (tiPRL-I or tiPRL-II) cDNA were constructed and the tiPRL-I and II proteins were produced in E. coli as inclusion bodies. These inclusion bodies were dissolved in 6 mol urea/l. Refolding of the proteins was followed by SDS-PAGE under non-reducing conditions so as to visualize the oxidized state of the molecules. Proteins tiPRL-I and tiPRL-II were purified by gel filtration and ion-exchange chromatography. The N-terminal sequence and bioactivities of both purified proteins were then analysed. Recombinant tiPRL-I and tiPRL-II induced a significant rise in plasma calcium levels as well as in mucocyte density in the abdominal skin epithelium. When tested on kidney membrane, both proteins exhibited potency in competing with 125I-labelled tiPRL-I for binding sites, but tiPRL-I seemed to be more potent than tiPRL-II in competing for these sites. The results obtained for the biological activities tested suggest that both recombinant prolactins were correctly refolded and had retained the full biological activity previously observed with the natural hormone preparations extracted from the animals. [less ▲]

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See detailPancreatic failure in relation to trauma and sepsis in intensive care patients
Lamy, Maurice ULg; Deby, Ginette ULg; Damas, Pierre ULg et al

in Applied Cardiopulmonary Pathophysiology [=ACP] (1991), 4

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