References of "Article"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailFailure to Infect Cats with Bovine Herpesvirus Type-4 Strain Movar 33/63
Thiry, Etienne ULg; Chappuis, G.; Bublot, M. et al

in Veterinary Record : Journal of the British Veterinary Association (1991), 128(26), 614-5

Full Text
Peer Reviewed
See detailCloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144
Feller, Georges ULg; Thiry, M.; Arpigny, J. L. et al

in Gene (1991), 102(1), 111-5

The cloning and expression of genes from a psychrotrophic bacterium in a mesophilic host are described. Three lipase (Lip)-encoding genes (lip) from the antarctic psychrotroph, Moraxella TA144, were ... [more ▼]

The cloning and expression of genes from a psychrotrophic bacterium in a mesophilic host are described. Three lipase (Lip)-encoding genes (lip) from the antarctic psychrotroph, Moraxella TA144, were cloned by inserting Sau3AI-generated DNA fragments into the BamHI site of the pSP73 plasmid vector. To prevent heat denaturation of the gene product, the screening procedure on agar plates containing an emulsified lipid involved growing of Escherichia coli recombinant colonies at 25 degrees C followed by incubation at 0 degree C. The three recombinant (reLip) were cell-associated and differed by their respective specificity towards p-nitrophenyl esters of various aliphatic chain lengths. These cloned reLip conserved the main character of the wild-type enzymes, i.e. a dramatic shift of the optimal temperature of activity towards low temperatures and pronounced heat lability. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailTemporal Control of Bovine Herpesvirus Type 4 Glycoprotein Synthesis
Dubuisson, J.; Pastoret, Paul-Pierre ULg; Thiry, Etienne ULg

in Journal of General Virology (1991), 72((Pt 6)), 1429-34

The glycoprotein gp6/gp10/gp17, gp11/VP24 and gp8 are major bovine herpesvirus type 4 antigens. The temporal expression of these glycoproteins was studied and it was shown that gp6/gp10/gp17 appeared as ... [more ▼]

The glycoprotein gp6/gp10/gp17, gp11/VP24 and gp8 are major bovine herpesvirus type 4 antigens. The temporal expression of these glycoproteins was studied and it was shown that gp6/gp10/gp17 appeared as early as 6 h post-inoculation (p.i.), and gp11/VP24 and gp8 were detected 8 h p.i. Moreover, a precursor of two components of gp6/gp10/gp17 glycoprotein [p(gp10/gp17)] was a beta-gamma protein, whereas the gp11/VP24 and gp8 glycoproteins were gamma proteins. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailEfficacy of Sustained-Release Vaginal Oestriol in Alleviating Urogenital and Systemic Climacteric Complaints
Foidart, Jean-Michel ULg; Vervliet, J.; Buytaert, Ph

in Maturitas (1991), 13(2), 99-107

In a double-blind, placebo-controlled study, 109 patients suffering from local and vasomotor postmenopausal complaints were randomly assigned to treatment with either depot vaginal suppositories ... [more ▼]

In a double-blind, placebo-controlled study, 109 patients suffering from local and vasomotor postmenopausal complaints were randomly assigned to treatment with either depot vaginal suppositories containing 3.5 mg oestriol (E3) or a placebo. The treatment schedule comprised one vaginal suppository twice weekly for 3 weeks initially, followed by maintenance therapy with one vaginal suppository weekly for the 6-month study period. The effectiveness of the therapy was assessed on the basis of questionnaires (Kupperman index for vasomotor complaints and an original urogenital index for local complaints) and gynaecological examinations which included assessments of vaginal cytology, vaginal pH and Doderlein bacilli. To rule out induced endometrial proliferation, endometrial biopsies were performed in 50 women before and after the study. The vaginal depot (E3) formulation showed highly significant superiority over the placebo with respect to therapeutic effect on local urogenital complaints and alleviation of vasomotor complaints, including hot flushes. Analysis of the endometrial biopsies indicated that the monotherapy used caused no endometrial stimulation. Taking into account the minimal rate of adverse effects, the 3.5 mg E3 depot formulation studied represents a useful variant in the range of preparations available for the treatment of post-menopausal complaints. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailInceste et abus de pouvoir
Glowacz, Fabienne ULg

in Perspectives : Revue sur les Enjeux Sociaux des Pratiques Psychologiques (1991), 18

Detailed reference viewed: 17 (6 ULg)
Peer Reviewed
See detailPhotosensitized Production of Singlet Oxygen by Merocyanine 540 Bound to Liposomes
Hoebeke, Maryse ULg; Piette, Jacques ULg; van de Vorst, A.

in Journal of Photochemistry and Photobiology B : Biology (1991), 9(3-4), 281-94

The production of singlet oxygen by merocyanine 540 was studied in dimyristoyl-phosphatidylcholine liposomes using two singlet oxygen probes: 9,10-anthracenedipropionic acid (water soluble) and 9,10 ... [more ▼]

The production of singlet oxygen by merocyanine 540 was studied in dimyristoyl-phosphatidylcholine liposomes using two singlet oxygen probes: 9,10-anthracenedipropionic acid (water soluble) and 9,10-dimethylanthracene (liposoluble). Upper and lower limits of singlet oxygen quantum yield for bound merocyanine 540 were determined to be 0.055 and 0.015 respectively. The diffusion characteristics of singlet oxygen were examined using the isotropic enhancement effect of D2O and the inhibitory effect of sodium azide. It was shown that 1O2 spent more than 87% of its lifetime in a vesicle environment. When the singlet-reacting substrate and the dye were both located in the bilayer, approximately 40% of the singlet oxygen remained in the liposomes where it was originally generated. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailActualités en médecine de l'appareil locomoteur. Vascularité urticarienne: à propos d'un cas
Wang, François-Charles ULg; de Marneffe, Pierre; Reginster, Jean-Yves ULg et al

in Revue Médicale de Liège (1991), 46(6), 336-42

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailCyclical Cushing's disease : a case report.
Velkeniers, B.; Beckers, Albert ULg; Stevenaert, Achille ULg et al

in Pathology - Research & Practice (1991), 187(5), 603-607

A 41-year-old man with clinical Cushing's syndrome and intermittent central ACTH hypersecretion for a period of 9 1/2 years follow-up is described. Episodes of biochemical and clinical remission ... [more ▼]

A 41-year-old man with clinical Cushing's syndrome and intermittent central ACTH hypersecretion for a period of 9 1/2 years follow-up is described. Episodes of biochemical and clinical remission alternated with periods of florid Cushing's disease, characterized by circadian hyperpulsatile ACTH and cortisol secretion. Responses to metyrapone and inhibition of ACTH and cortisol hypersecretion after high dose dexamethasone during active phases of the disease favored a central origin of ACTH hypersecretion, confirmed by simultaneous bilateral venous sampling of the sinus petrosus inferior. Prolonged clinical remission followed near total anterior hypophysectomy. However, on anatomopathological examination of the pituitary neither corticotroph cell hyperplasia nor a microadenoma could be documented. The possibility of a functional ACTH hypersecretion is discussed. [less ▲]

Detailed reference viewed: 22 (2 ULg)
Peer Reviewed
See detailQuantitative Buffy Coat Analysis Related to Adrenocortical Function in Horses During a Three-Day Event Competition
Linden, Annick ULg; Art, Tatiana ULg; Amory, Hélène ULg et al

in Zentralblatt für Veterinarmedizin. Reihe A (1991), 38(5), 376-82

This study was carried out to investigate stress induced in a cross-country by measuring plasma cortisol and to compare the changes in cortisol level with the leucocytic changes measured with the ... [more ▼]

This study was carried out to investigate stress induced in a cross-country by measuring plasma cortisol and to compare the changes in cortisol level with the leucocytic changes measured with the quantitative buffy coat (QBC) analysis, which is a new method applicable in field conditions for the evaluation of haematological parameters in horses. Seven healthy horses competing in a national three-day event were investigated. Venous blood was sampled under resting condition, 2 min and 180 min after the completion of the cross-country and analysed for haematological parameters, plasma cortisol, protein and lactate levels. Immediately after the event, there was a significant increase in all values except in the granulocytes (Gr) to lymphocytes-monocytes (LM) ratio. 180 min after the event, all the parameters returned to their resting levels while white blood cells (WBC), Gr and Gr/LM ratio were significantly higher with respect to their resting values. A high correlation (r = 0.82) was found between the relative increase in plasma cortisol levels 2 min after exercise and the relative increase in Gr/LM 180 min after exercise. Therefore it was concluded that the Gr/LM ratio measured 180 min after exercise with the QBC analysis is a reliable index to estimate the plasma cortisol levels measured 2 min after exercise. This new technique could consequently be used in order to evaluate, in field conditions, the exercise-induced stress. [less ▲]

Detailed reference viewed: 53 (3 ULg)
Peer Reviewed
See detailTgf-beta1 Modulates bFGF Receptor Message Expression in Cultured Adult Auditory Neurons
Lefebvre, P. P.; Staecker, H.; Weber, T. et al

in Neuroreport (1991), 2(6), 305-8

Basic fibroblast growth factor (bFGF) has been shown to have neuronotrophic effects on cultured neurons. Transforming growth factor beta (TGFss1) has been implicated in the modulation of cellular ... [more ▼]

Basic fibroblast growth factor (bFGF) has been shown to have neuronotrophic effects on cultured neurons. Transforming growth factor beta (TGFss1) has been implicated in the modulation of cellular receptors for bFGF in several cell types. In this study, we show that TGFss1 is expressed in cultured adult mouse auditory neurons in response to explanation injury and acts in an autocrine fashion to increase the level of expression of bFGF receptors message in these same neurons. Based on these in-vitro results, we propose that these trophic factors (i.e. TGFss1 and bFGF) play a significant role in the response to injury by the mature auditory system. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailExperimental and Numerical Approaches of Contact with Friction and Wear in Large Deformation
Cescotto, Serge ULg; Charlier, Robert ULg; Bricout, J. P. et al

in European Journal of Mechanical Engineering (1991), 36(n° 2),

Detailed reference viewed: 6 (2 ULg)
Peer Reviewed
See detailExpression of Laminin by Human Fibroblasts, Ht1080 Fibrosarcoma Cells and Mcf-7 Breast Adenocarcinoma Cells. Lack of Regulation by the Cell Density and Extracellular Matrix
Munaut, Carine ULg; Noël, Agnès ULg; Sobel, M. et al

in Cell Biology International Reports (1991), 15(6), 499-509

We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 cells on various substrates (plastic, collagen type I, laminin). All cell types used adhered on the three ... [more ▼]

We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 cells on various substrates (plastic, collagen type I, laminin). All cell types used adhered on the three substrates with, however, a delayed attachment on laminin. On all substrates, cell grew as monolayer with the exception of MCF-7 cells that formed clusters on laminin. The epithelial MCF-7 cells as well as mesenchymal cells (fibroblasts and tumoral HT1080 cells) synthesized laminin and expressed mRNA coding for laminin B1 chain and for the 67 kD laminin binding protein. The levels of these mRNAs were not modulated by culture conditions which affect cell morphology nor by cell density. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailNucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues
Feller, Georges ULg; Thiry, M.; Gerday, Charles ULg

in DNA & Cell Biology (1991), 10(5), 381-8

The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a ... [more ▼]

The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a predicted Mr of 47,222. This enzyme contains a Ser-centered consensus sequence and a conserved His-Gly dipeptide found in most lipase amino-terminal domains. These sequences are involved in the lipase active site conformation since substitution of the conserved Ser or His residues by Ala and Gln, respectively, results in the loss of both lipase and esterase activities. Structural factors that would allow proper enzyme flexibility at low temperatures are discussed. It is suggested that only subtle changes in the primary structure of these psychrotrophic enzymes can account for their ability to catalyze lipolysis at temperatures close to 0 degrees C. [less ▲]

Detailed reference viewed: 17 (2 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 5. Synthesis and characterization of diblock copolymers based on poly-ε-caprolactone and poly(L,L or D,L)lactide by aluminum alkoxides
Jacobs, C.; Dubois, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1991), 24(11), 3027-3034

Aluminum isopropoxide is known to be an effective initiator for the ring-opening polymerization of lactides (D,L and L,L) and epsilon-caprolactone (epsilon-CL). For a question of mutual reactivity, the ... [more ▼]

Aluminum isopropoxide is known to be an effective initiator for the ring-opening polymerization of lactides (D,L and L,L) and epsilon-caprolactone (epsilon-CL). For a question of mutual reactivity, the sequential polymerization of these two comonomers can only be achieved when epsilon-CL is first polymerized followed by the lactide. Formation of a large amount of homo PLA is however observed and has been attributed to a great difference in the mean degree of association of aluminum alkoxides in toluene in the presence of epsilon-CL and LA. In toluene, the mean number (n) of active sites per Al(O(i)Pr)3 molecule jumps from 1 to 3 when epsilon-CL is substituted by LA. The addition of a small amount of an alcohol, like 2-propanol, is effective in preventing the aluminum alkoxide from associating, and n is then 3 whatever the monomer used. 2-Propanol participates in the polymerization of epsilon-CL and LA as supported by a decrease in the molecular weight of the final polymer in relation to the molar amount of added alcohol. This means that the alcohol molecules are rapidly exchanged with the alkoxide groups on Al. Under such conditions, the formation of pure diblock copolymers P(epsilon-CL-b-LA) is reported. Another successful strategy for the synthesis of the diblock copolymer consists in using an Al derivative that bears only one alkoxide group to initiate the polymerization of the two cyclic esters. In this regard, a diethylaluminum alkoxide is of great value, particularly the ethoxide one. A third, conceptually similar, approach to the synthesis of P(epsilon-CL-b-LA) copolymers relates to the reaction of a performed hydroxy-terminated PCL with triethylaluminum and the use of that macroinitiator in the ring-opening polymerization of the lactide. [less ▲]

Detailed reference viewed: 23 (3 ULg)
Full Text
Peer Reviewed
See detailGround-Based Infrared Measurements of HNO3 Total Column Abundances: Long-Term Trend and Variability
Rinsland, C.P.; Zander, Rodolphe ULg; Demoulin, Philippe ULg

in Journal of Geophysical Research (1991), 96(D5), 9379-9389

The long-term trend and variability of the total column amount of atmospheric nitric acid (HNO3) have been investigated based on time series of infrared solar absorption spectra recorded at two remote ... [more ▼]

The long-term trend and variability of the total column amount of atmospheric nitric acid (HNO3) have been investigated based on time series of infrared solar absorption spectra recorded at two remote high-altitude sites, the International Scientific Station of the Jungfraujoch (ISSJ) in the Swiss Alps (altitude 3.6 km, latitude 46.5°N, longitude 8.0°E) and the National Solar Observatory McMath solar telescope facility on Kitt Peak (altitude 2.1 km, latitude 31.9°N, longitude 111.6°W), southwest of Tucson, Arizona. The HNO3 v5 band Q branch at 879.1 cm-1 and three P branch manifolds near 869 cm-1 were analyzed using a nonlinear least squares spectral fitting technique and a consistent set of spectroscopic line parameters. The ISSJ measurements evaluated in the present work consist of two solar spectra recorded with a grating spectrometer in June 1951 and a set of observations obtained with a high-resolution Fourier transform spectrometer between June 1986 and June 1990. The modern ISSJ measurements show a ~20% peak-to-peak amplitude seasonal cycle with a winter maximum superimposed on significant variability and a summer minimum; the June results from 1986 to 1990 are both higher and lower than the two retrieved June 1951 HNO3 total column amounts. The fitted trend, (-0.16 +- 0.50) %/yr, 2 sigma, indicates that there has been no detectable change in the HNO3 total column over the last 4 decades. The Kitt Peak measurements, recorded with a high-resolution Fourier transform spectrometer between December 1980 and June 1990, also show marked variability in the HNO3 total column, but in contrast to the ISSJ measurements, no obvious seasonal cycle is observed. The deduced trend in the total column above Kitt Peak, (-0.8 +- 1.6) %/yr, 2 sigma, is consistent with the ISSJ time series of measurements, in that no significant HNO3 long-term trend has been found. The sets of measurements from the two sites are compared with each other and with previously published results, with emphasis on the reported variability of HNO3 and the changes in the HNO3 total column with season and latitude. [less ▲]

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailA standard numbering scheme for the class A beta-lactamases
Ambler, R. P.; Coulson, A. F. W.; Frère, Jean-Marie ULg et al

in Biochemical Journal (1991), 276(Pt 1), 269-270

Detailed reference viewed: 25 (2 ULg)
Peer Reviewed
See detailEmg Activity in Pericranial Muscles During Postural Variation and Mental Activity in Healthy Volunteers and Patients with Chronic Tension Type Headache
Schoenen, Jean ULg; Gérard, P.; De Pasqua, Victor ULg et al

in Headache (1991), 31(5), 321-4

EMG activity was recorded over frontalis, temporalis and trapezius muscles in a supine position, a standing position and during a mental task in 32 female patients suffering from chronic tension-type ... [more ▼]

EMG activity was recorded over frontalis, temporalis and trapezius muscles in a supine position, a standing position and during a mental task in 32 female patients suffering from chronic tension-type headache and in 20 healthy volunteers. Measurements in patients were made before and after biofeedback therapy. All EMG levels were on average significantly higher in patients than in controls. 62.5% of patients had at least one abnormal EMG level, but only 34% were beyond the normal range, if 1 muscle and 1 recording condition was considered. EMG levels were not correlated with headache severity, anxiety or response to biofeedback treatment. It is therefore suggested that pericranial EMG activity is not pathogenetic in chronic tension type headache, but merely one of several pathophysiologic changes, that are produced by a central dysfunction. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
See detailLes auto-anticorps et le profil immunopathologique des lupus érythémateux
Nikkels, Arjen ULg; Tassoudji, Nazli ULg; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (1991), 46(5), 279-285

Nous présentons une synthèse de la signification diagnostique, clinique et pronostique d'une série d'auto-anticorps apparaissant dans les diverses formes de lupus érythémateux.

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailDéveloppement embryonnaire caprin in vitro: étude des conditions de culture
Fieni, Francis; Buggin, M.; Tainturier, Daniel et al

in Recueil de Médecine Vétérinaire (1991), 167(5), 435-444

Three culture Media: PBS; HAM F10 and B2 Menezo were used to compare and study the in vitro development of goats embryos. After 48h of culture in these media, the rate of Morulas developed was o%, 19% and ... [more ▼]

Three culture Media: PBS; HAM F10 and B2 Menezo were used to compare and study the in vitro development of goats embryos. After 48h of culture in these media, the rate of Morulas developed was o%, 19% and 42% respectively, and that of blastocysts was 25%, 73% and 95%. The B2 Menezo medium would therefore appear well adapted to the culture of blastocysts. The morulas, however, irrespective of the medium used showed limited development. [less ▲]

Detailed reference viewed: 161 (2 ULg)
Full Text
Peer Reviewed
See detailPlasma Vitamin E, Total Lipids and Myeloperoxidase Levels During Spinal Surgery. A Comparison between Two Anesthetic Agents: Propofol and Isoflurane
Hans, Pol ULg; Canivet, Jean-Luc ULg; Pincemail, Joël ULg et al

in Acta Anaesthesiologica Scandinavica (1991), 35(4), 302-5

Plasma levels of vitamin E (Vit. E), total lipids (TL), Vit. E to TL ratio and myeloperoxidase (MPO) were studied in 20 patients undergoing lumbar spinal surgery and randomly allocated to two anesthetic ... [more ▼]

Plasma levels of vitamin E (Vit. E), total lipids (TL), Vit. E to TL ratio and myeloperoxidase (MPO) were studied in 20 patients undergoing lumbar spinal surgery and randomly allocated to two anesthetic groups: propofol (bolus dose + continuous infusion and thiopental/isoflurane. Peripheral blood samples were withdrawn prior to induction, each hour during anesthesia and 1 h after the end of surgery. Mean Vit. E and TL levels as well as mean Vit. E to TL ratios remained in the normal range over the entire period of study whatever the anesthetic regimen. MPO levels rose significantly in the post-operative period only, but without statistical difference between the two groups. Therefore, anesthesia with propofol or thiopental/isoflurane modifies neither total lipid concentrations nor plasma Vit. E, which is a potent endogenous inhibitor of lipid peroxidation bound to lipoproteins. The rise of plasma MPO suggests a moderate post-operative neutrophil activation which is not influenced by anesthetic techniques. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Peer Reviewed
See detailEconomic and Production Consequences of Liver Fluke Disease in Double-Muscled Fattening Cattle
Genicot, Bruno; Mouligneau, Frédéric; Lekeux, Pierre ULg

in Zentralblatt für Veterinarmedizin. Reihe B = Journal of Veterinary Medicine. Series B (1991), 38(3), 203-208

The frequency of liver fluke disease in fattening units was determined by the analysis of random faeces samples issued from 1,513 Belgian White Blue bulls aged from 5 to 7 months and weighing from 200 to ... [more ▼]

The frequency of liver fluke disease in fattening units was determined by the analysis of random faeces samples issued from 1,513 Belgian White Blue bulls aged from 5 to 7 months and weighing from 200 to 300 kg. 12.5% of the investigated bulls were positive for liver fluke disease. These bulls were spread over 56.5% of the investigated fattening units. Furthermore the infestation rate varied from 0 to 33.3% inside the fattening units. In order to assess the economic consequences of bovine fascioliasis in double-muscled cattle and the beneficial effects of a treatment against such a disease, a trial including 30 Belgian White Blue bulls, weighing 365 +/- 9 kg and aged from 10 to 12 months, was conducted in a selected fattening unit. On the basis of faecal examinations, the 30 animals were subdivided in negative (group A; n = 10) and positive animals (n = 20) for fascioliasis, the latter being either treated with nitroxinil (group B; n = 10) or not (group C; n = 10) on day 0 of this trial which was conducted during 75 days. The daily body gains in group C (1.661 +/- 0.140 kg) were significantly lower than those in group A (1.975 +/- 0.120 kg). On the other hand there was no significant difference between the daily body gains registered in group B (1.960 +/- 0.085 kg) and A. The estimated financial loss, due to flukes and accounted on a 75 day-period, averaged 2,748 Belgian Francs per bull in group C.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 33 (1 ULg)
Full Text
Peer Reviewed
See detailBiosynthesis of the 67 kDa high affinity laminin receptor.
Castronovo, Vincenzo ULg; Claysmith, A. P.; Barker, K. T. et al

in Biochemical and Biophysical Research Communications (1991), 177(1), 177-83

High affinity interactions between cells and laminin are mediated, at least in part, by the 67 kDa laminin receptor (67 LR). A 37 kDa nascent polypeptide (37 LRP), predicted by a full length cDNA clone ... [more ▼]

High affinity interactions between cells and laminin are mediated, at least in part, by the 67 kDa laminin receptor (67 LR). A 37 kDa nascent polypeptide (37 LRP), predicted by a full length cDNA clone and obtained by in vitro translation of hybrid-selected laminin receptor mRNA, has been immunologically identified in cancer cell extracts as the putative precursor of the 67 LR. In this study, we used affinity purified antibodies developed against cDNA-deduced 37 LRP synthetic peptides in pulse chase experiments and demonstrated a precursor-product relationship between the 37 LRP and the 67 LR. Immunoblot, pulse chase and immunofluorescence experiments showed that transient transfection of the full length 37 LRP cDNA clone induced a dramatic increase in the synthesis of the 37 LRP but not of the mature 67 LR. We propose that the 67 LR results from the association of two gene products: the 37 LRP and a polypeptide yet to be identified. [less ▲]

Detailed reference viewed: 7 (2 ULg)
Peer Reviewed
See detailEconomic and Production Repercussions of Deworming by Pulse Release Device and Oral Suspension in Grazing Double-Muscled Fattening Cattle
Genicot, Bruno; Mouligneau, Frédéric; Lekeux, Pierre ULg

in Zentralblatt für Veterinarmedizin. Reihe B = Journal of Veterinary Medicine. Series B (1991), 38(3), 238-240

In this field trial, the repercussions of 2 administration forms of oxfendazole, namely a single administration of a front-loaded device (group 1; n = 18) and a repeated administration of a 90.6 per cent ... [more ▼]

In this field trial, the repercussions of 2 administration forms of oxfendazole, namely a single administration of a front-loaded device (group 1; n = 18) and a repeated administration of a 90.6 per cent oral suspension (group 2; n = 18), were compared in first season-grazing double-muscled fattening bulls. The comparison was based on the production performances, the mean worm egg counts and the product- and labour-costs. The daily body gains determined on the whole trial period, i.e. 1.312 +/- 0.055 kg and 1.270 +/- 0.056 kg for groups 1 and 2 respectively, and mean worm egg counts per gram (on days 0 and 130:200.0 +/- 44.3 and 94.4 +/- 27.4 for group 1; 366.7 +/- 98.4 and 100.0 +/- 33.6 for group 2) did not differ significantly between the two groups. However, the total costs were lower with a front-loaded device (1,021 Belgian Francs) than with oral suspension (1,248 Belgian Francs per bull). [less ▲]

Detailed reference viewed: 9 (1 ULg)
Peer Reviewed
See detailExteroceptive Silent Period of Temporalis Muscle in Menstrual Headaches
Schoenen, Jean ULg; Bottin, D.; Sulon, J. et al

in Cephalalgia : An International Journal of Headache (1991), 11(2), 87-91

The second exteroceptive silent period (ES2) of temporalis muscle was recorded on days 1 and 15 of the menstrual cycle in 17 women, 9 of whom suffered regularly from tension-type headaches during ... [more ▼]

The second exteroceptive silent period (ES2) of temporalis muscle was recorded on days 1 and 15 of the menstrual cycle in 17 women, 9 of whom suffered regularly from tension-type headaches during menstruation. Mean duration of temporalis ES2 was significantly shorter on day 1 of the cycle than on day 15. This difference was due to a marked menstrual reduction of ES2 in the headache subgroup. A positive correlation was found between ES2 durations and oestradiol/progesterone ratios. We hypothesize that the variations of ES2 during the ovarian cycle result from the modulatory effects of oestrogens on descending aminergic pathways that control excitability of inhibitory brainstem interneurons mediating exteroceptive suppression of jaw-closing motoneurons. [less ▲]

Detailed reference viewed: 41 (2 ULg)
Full Text
Peer Reviewed
See detailComparison of Proteins of Simian Herpesvirus Aotus Type 2 and Bovine Herpesvirus Type 4
Dubuisson, J.; Danyi, Sophie ULg; Bublot, M. et al

in Journal of General Virology (1991), 72((Pt 5)), 1145-50

Genomes of herpesvirus aotus type 2 (HVA-2) and bovine herpesvirus type 4 (BHV-4) have previously been shown to be closely similar. Moreover, preliminary serological data indicated that HVA-2 is ... [more ▼]

Genomes of herpesvirus aotus type 2 (HVA-2) and bovine herpesvirus type 4 (BHV-4) have previously been shown to be closely similar. Moreover, preliminary serological data indicated that HVA-2 is antigenically related to BHV-4. To extend this study, structural components of four BHV-4 strains and HVA-2 were compared by SDS-PAGE, radioimmunoprecipitation and Western blotting. The overall pattern of structural proteins was the same for HVA-2 and BHV-4 but variations were observed in electrophoretic profiles of glycoproteins, mainly of the two major ones (gp6/gp10/gp17 and gp11/VP24). Variations between HVA-2 and BHV-4 glycoproteins were greater than those observed among BHV-4 strains. [less ▲]

Detailed reference viewed: 7 (1 ULg)
Peer Reviewed
See detailBlood Magnesium Levels in Migraine
Schoenen, Jean ULg; Sianard-Gainko, J.; Lenaerts, M.

in Cephalalgia : An International Journal of Headache (1991), 11(2), 97-9

Serum and erythrocyte magnesium levels were screened between attacks in patients with migraine without aura (n = 38) and with aura (n = 6), and for comparison in a group of patients suffering from chronic ... [more ▼]

Serum and erythrocyte magnesium levels were screened between attacks in patients with migraine without aura (n = 38) and with aura (n = 6), and for comparison in a group of patients suffering from chronic tension-type headache (n = 25) as well as a group of neurological, non-headache patients (n = 19). Serum magnesium levels were not significantly different between the four groups of patients. In contrast, magnesium in erythrocytes was on average significantly reduced in patients with migraine without aura compared to the other groups. It is hypothesized that this reduction might be due to an abnormal regulation of intracellular magnesium possibly reflecting at the periphery changes observed in the brain of migraineurs. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 4. Mechanism and kinetics of lactide homopolymerization by aluminum isopropoxide
Dubois, Philippe; Jacobs, R.; Jérôme, Robert ULg et al

in Macromolecules (1991), 24(9), 2266-2270

Aluminum isopropoxide is an effective initiator for the polymerization of lactides in toluene at 70-degrees-C. The ring-opening polymerization proceeds through a "coordination-insertion" mechanism and ... [more ▼]

Aluminum isopropoxide is an effective initiator for the polymerization of lactides in toluene at 70-degrees-C. The ring-opening polymerization proceeds through a "coordination-insertion" mechanism and selective rupture of the acyl oxygen bond of the monomer. A kinetic study has shown that the polymerization is first order in both the monomer and initiator. The polymerization is typically "living" until a molecular weight of ca. 90 000 is reached. At higher temperatures (100-degrees-C), inter- and intramolecular transesterification reactions are responsible for a limitation in the molecular weight of the growing chains. In toluene, at 70-degrees-C, each alkoxide of the aluminum isopropoxide participates in the polymerization. Any molecular weight below 90 000 can accordingly be predicted on the basis of the monomer/Al molar ratio, and the polydispersity is rather narrow (1.1-1.4). [less ▲]

Detailed reference viewed: 29 (1 ULg)
Peer Reviewed
See detailEffects of Growth on Functional and Morphological Echocardiographic Variables in Friesian Calves
Amory, Hélène ULg; Lekeux, Pierre ULg

in Veterinary Record : Journal of the British Veterinary Association (1991), 128(15), 349-54

For echocardiography to become a reliable tool for the diagnosis, monitoring and prognosis of cardiac diseases in cattle, it is necessary to determine normal values of echocardiographic variables and ... [more ▼]

For echocardiography to become a reliable tool for the diagnosis, monitoring and prognosis of cardiac diseases in cattle, it is necessary to determine normal values of echocardiographic variables and their changes during the growth of healthy animals. In this study, 53 echocardiographic protocols were collected from 17 healthy Friesian calves during their period of growth. The protocol consisted of the measurement or calculation of 10 dimensional and six functional variables from M-mode long axis views of the heart. The relationships between these variables and age, body-weight and body surface area were analysed. Most of the dimensional parameters increased significantly with body size and were best predicted by an allometric regression. However, the functional indices did not change with growth, and the normal values obtained in this study may be applied to calves of any body weight. [less ▲]

Detailed reference viewed: 5 (1 ULg)
Full Text
Peer Reviewed
See detailAbundances of neutron capture elements in metal-poor dwarfs. I - Yttrium and zirconium
Zhao, G.; Magain, Pierre ULg

in Astronomy and Astrophysics (1991), 244

The yttrium and zirconium abundances are determined in a sample of 20 metal-poor stars on the basis of high resolution, high SNR spectra. Significant differences between the behaviors of these two ... [more ▼]

The yttrium and zirconium abundances are determined in a sample of 20 metal-poor stars on the basis of high resolution, high SNR spectra. Significant differences between the behaviors of these two neighboring elements are found, zirconium being less deficient than yttrium in Population II stars. Moreover, there is a genuine cosmic scatter in the abundances of these two elements relative to iron, of the order of 20 percent. The scatter is lower when these elements are compared to titanium, which might indicate that a significant fraction of the lighter neutron-capture elements are produced in massive stars. [less ▲]

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailThe very massive star HDE269676 and its components
Heydari-Malayeri, M.; Hutsemekers, Damien ULg

in Astronomy and Astrophysics (1991), 244

It is shown that one of the most luminous stars of the LMC, HDE269676, is in fact a massive star cluster. On the basis of observations with the ESO New Technology Telescope (NTT) the main components of ... [more ▼]

It is shown that one of the most luminous stars of the LMC, HDE269676, is in fact a massive star cluster. On the basis of observations with the ESO New Technology Telescope (NTT) the main components of the cluster are classified and it is shown that there are several O type stars in HDE269676 instead of the one or two previously known. This is interesting for the initial mass function in the LMC and implies that the number of O type stars in the LMC is generally underestimated. The most massive star of the system being about a factor three less luminous than previously estimated is a super-giant O5f with a zero age main sequence mass of about 80 solar. [less ▲]

Detailed reference viewed: 13 (2 ULg)
Peer Reviewed
See detailTransfert des insulines U40 aux insulines U100: comparaison des profils insulinémiques et glycémiques chez le patient diabétique
Scheen, A. J.; Guiot, Julien; Stassen, M. P. et al

in Revue Médicale de Liège (1991), 46(4), 181-7

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailAdenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
Scippo, Marie-Louise ULg; Beckers, Albert ULg; Frankenne, Francis ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1991), 99(2), 135-140

We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products ... [more ▼]

We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products (immunohistochemistry). The GH-secreting tumor was shown to express the genes coding for GH and PRL and to synthesize the corresponding hormones. In the cases of prolactinomas, immunohistochemical data demonstrated the synthesis of prolactin only. In addition to the PRL gene, Northern blot analysis revealed the transcription of the alpha-subunit gene in one case. Hormone genes were found to be expressed in 7 out of the 10 silent tumors, whereas no hormone synthesis was detected in any of these tissues. LH-beta mRNA was found in 3 cases, FSH-beta mRNA in 5 cases and alpha-subunit gene was shown to be expressed in one case. Surprisingly, the level of expression of the FSH-beta-gene was higher than in normal tissue. This study confirms that some so called << silent >> adenomas are expressing alpha- and/or beta-subunit glycoprotein hormone genes, even if no hormone is synthesized. The therapeutic action of bromocriptine described in some << silent >> adenomas cases could be related to that hormone gene expression potentiality. [less ▲]

Detailed reference viewed: 16 (2 ULg)
Peer Reviewed
See detailAt the Cutting Edge. Biosynthesis and Paracrine/Cryptocrine Actions of 'Self' Neurohypophysial-Related Peptides in the Thymus
Geenen, Vincent ULg; Robert, F.; Martens, Henri ULg et al

in Molecular & Cellular Endocrinology (1991), 76(1-3), 27-31

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailSeasonal Cycle and Secular Trend of the Total and Tropospheric Column Abundance of Ethane Above the Jungfraujoch
Ehhalt, D.H.; Schmidt, U.; Zander, Rodolphe ULg et al

in Journal of Geophysical Research (1991), 96(D3), 4985-4994

Total column abundances of ethane (C2H6) above the Jungfraujoch Station, Switzerland, 3.58-km altitude, have been deduced from infrared solar spectra recorded in 1951 and from 1984 to 1988. The results ... [more ▼]

Total column abundances of ethane (C2H6) above the Jungfraujoch Station, Switzerland, 3.58-km altitude, have been deduced from infrared solar spectra recorded in 1951 and from 1984 to 1988. The results were derived from the spectroscopic analysis of the v7 band PQ3, PQ1, and RQ0 subbranches of C2H6 near 2976.8, 2983.4, and 2986.7 cm-1, respectively. The 1984-1988 results obtained from more than 300 observations at 0.005 cm-1 resolution show a definite seasonal variation in the total vertical column abundance of that gas, with a maximum of (1.43 +- 0.03) x 10E16 molecules/cm2 during March and April and a minimum in the fall. The corresponding C2H6 mixing ratio in the free troposphere was derived to 1.53 +- 0.14 ppb in early April; the ratio of maximum to minimum C2H6 concentration in the troposphere was found to be 1.88 +- 0.12. From the analysis of the same spectral features observed in March and April 1951, we have deduced a total vertical column abundance of (1.09 +- 0.10) x 10E16 molecules/cm2. Assuming an exponential increase with time and correcting for a small decrease in the stratospheric amount of C2H6 due to the man-induced increase in stratospheric chlorine, we obtain a secular trend in the tropospheric burden above the Jungfraujoch equal to (0.85 +- 0.3)%/yr. It is argued that this trend is valid for the entire northern hemisphere. [less ▲]

Detailed reference viewed: 6 (3 ULg)
Full Text
Peer Reviewed
See detailGrafts of Syngenic Cultured, Adult Dorsal Root Ganglion-Derived Schwann Cells to the Injured Spinal Cord of Adult Rats: Preliminary Morphological Studies
Martin, Didier ULg; Schoenen, Jean ULg; Delree, P. et al

in Neuroscience Letters (1991), 124(1), 44-8

Highly enriched cultures of Schwann cells were obtained from adult rat dorsal root ganglia and implanted (5 x 10(5) -9 x 10(5) cells) in the spinal cord of syngenic adult rats at the site of an acute ... [more ▼]

Highly enriched cultures of Schwann cells were obtained from adult rat dorsal root ganglia and implanted (5 x 10(5) -9 x 10(5) cells) in the spinal cord of syngenic adult rats at the site of an acute compression lesion produced by a subdural inflatable microballoon. These autografts survived and invaded the host tissue, reducing central cavitation and astrocytic gliosis. They dramatically promoted ingrowth of axons, the majority of which appeared to come from the dorsal roots as judged by their neuropeptide content. Invasion of the transplants by descending, e.g. aminergic fibers, was negligible at survival times of up to 4 months. Nonetheless, autologous Schwann cells, which are readily available in the host, represent a promising material for grafts into the injured spinal cord. [less ▲]

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailMacromolecular Engineering of polylactones and polylactides. 3. Synthesis, characterization and applications of poly(ε-caprolactone) macromonomers
Dubois, Philippe; Jérôme, Robert ULg; Teyssié, Philippe

in Macromolecules (1991), 24(5), 977-981

Well-defined omega-methacryloylpoly(epsilon-caprolactone) (or PCL) macromonomers have been prepared by using an aluminum alkoxide that bears a methacrylic double bond. The initiator results from the ... [more ▼]

Well-defined omega-methacryloylpoly(epsilon-caprolactone) (or PCL) macromonomers have been prepared by using an aluminum alkoxide that bears a methacrylic double bond. The initiator results from the equimolar reaction of triethylaluminum with 2-hydroxyethyl methacrylate (HEMA). The coordination-insertion type of polymerization is living and yields exclusively linear macromonomers of a predictable molecular weight with a narrow molecular weight distribution. IR and H-1 NMR studies show that the methacryloyl group associated with the active alkoxy group of the initiator is selectively attached to one chain end. Should the living polymerization be terminated by methacryloyl chloride, and a alpha,omega-dimethacryloyl-PCL, i.e. a alpha,omega-macromonomer is obtained. PCL macromonomers and HEMA are easily copolymerized in the presence of AIBN, resulting in a hydrophilic poly(HEMA) backbone grafted with hydrophobic PCL subchains. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Peer Reviewed
See detailNature of the Variability of VV Scl
Renson, Pierre ULg

in Information Bulletin on Variable Stars (1991), 3568

Not Available

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailThe most massive star of the largest H II region in the Small Magellanic Cloud
Heydari-Malayeri, M.; Hutsemekers, Damien ULg

in Astronomy and Astrophysics (1991), 243

It is shown that the brightest star in the most important H II region of the Small Magellanic Cloud (SMC) is a multiple system. The zero-age main sequence mass of this star drops significantly from the ... [more ▼]

It is shown that the brightest star in the most important H II region of the Small Magellanic Cloud (SMC) is a multiple system. The zero-age main sequence mass of this star drops significantly from the previously estimated value of 130 or 110 solar masses to less than 85 solar masses, while its present mass falls from 113 to 58 solar masses, both due to multiplicity and extinction correction. This is another piece of evidence against the existence of stars more massive than about 100 solar masses in the SMC. It is also shown that the present mass of one of the two known O3 stars in the SMC is significantly smaller than presently believed. [less ▲]

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailWR 22 is an Eclipsing Binary Star
Gosset, Eric ULg; Remy, Marc; Manfroid, Jean ULg et al

in Information Bulletin on Variable Stars (1991), 3571

Not Available

Detailed reference viewed: 4 (0 ULg)
Full Text
See detailAutomatic Photometry at La Silla
Sterken, C.; Manfroid, Jean ULg

in The Messenger (1991), 63

Not Available

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailLong-term photometry of variables at ESO. I - The first data catalogue (1982-1986)
Manfroid, Jean ULg; Sterken, C.; Bruch, A. et al

in Astronomy and Astrophysics. Supplement Series (1991), 87

This paper presents the catalog of photometric data in the Stromgren system obtained during the first four years (October 1982 - September 1986) of the Long-Term Photometry of Variables (LTPV) program at ... [more ▼]

This paper presents the catalog of photometric data in the Stromgren system obtained during the first four years (October 1982 - September 1986) of the Long-Term Photometry of Variables (LTPV) program at ESO. The data are available in computer-readable form. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailLe système immunitaire cutané et le photovieillissement
Nikkels, Arjen ULg; Tassoudji, Nazli ULg; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (1991), 46(3), 158-163

La connaissance du système immunitaire cutané a beaucoup progressé durant les dernières années. Considérée auparavant comme un organe passif de protection, on sait aujourd'hui que la peau est un organe ... [more ▼]

La connaissance du système immunitaire cutané a beaucoup progressé durant les dernières années. Considérée auparavant comme un organe passif de protection, on sait aujourd'hui que la peau est un organe immunocompétent. Le vieillissement cutané et l'exposition à la lumière ultraviolette ont une influence importante sur le système immunitaire cutané. Après une brève actualisation et description des cellules immunocompétentes de l'épiderme et du derme, nous considérerons la modulation du système immunitaire cutané liée au photovieillissement. [less ▲]

Detailed reference viewed: 42 (2 ULg)
Full Text
Peer Reviewed
See detailRecherche fondamentale et médecine
Ghuysen, Jean-Marie ULg

in Revue Médicale de Liège (1991), 46(3), 115-122

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailAbsence of Laminin Deposition in Breast Cancer and Metastases except to the Brain
Castronovo, Vincenzo ULg; Bracke, M. E.; Mareel, M. M. et al

in Pathology - Research & Practice (1991), 187(2-3), 201-8

Laminin, a major glycoprotein of basement membrane has been found to play significant roles during invasion and metastases. In this study, we have examined the distribution of laminin in several human ... [more ▼]

Laminin, a major glycoprotein of basement membrane has been found to play significant roles during invasion and metastases. In this study, we have examined the distribution of laminin in several human brain carcinoma metastases, human breast cancers, skin and lymph node metastases of breast cancer as well as in an in vitro and an in vivo model of invasion. A laminin accumulation was demonstrated a) at the border between human metastatic carcinoma cells and surrounding neural tissue; b) at the invasive edge between MO4 cells (a highly malignant cell line which synthesizes large amounts of laminin) and host tissues of syngenic mice; c) at the front of invasion between MO4 cells and precultured heart fragments in an in vitro model of invasion. Laminin, but not type IV collagen, promoted attachment of MO4 cells. This attachment was inhibited by preincubation of laminin matrix support with (+)-catechin, a flavonoid which also prevented invasion of the precultured heart fragment in vitro. Our data demonstrate that laminin accumulates between malignant cells and host tissue in human brain metastases and in an in vitro and an in vivo model of invasion. In these later models, accumulation of laminin is the consequence, at least in part, of its biosynthesis by MO4 cells. Since laminin promotes attachment of malignant cells in vitro, increases invasiveness and metastatic activities of murine malignant cells, it is tempting to speculate that laminin synthesized by invasive cells and accumulated at the front of invasion plays a significant role in the first step of invasion. [less ▲]

Detailed reference viewed: 42 (4 ULg)
Full Text
Peer Reviewed
See detailAntigenic and Genomic Identity between Simian Herpesvirus Aotus Type 2 and Bovine Herpesvirus Type 4
Bublot, M.; Dubuisson, J.; Van Bressem, M. F. et al

in Journal of General Virology (The) (1991), 72((Pt 3)), 715-9

Herpesvirus aotus type 2 (HVA-2) was isolated from a culture of kidney cells from a healthy owl monkey (Aotus trivirgatus). Bovine herpesvirus type 4 (BHV-4) is frequently isolated from diseased and even ... [more ▼]

Herpesvirus aotus type 2 (HVA-2) was isolated from a culture of kidney cells from a healthy owl monkey (Aotus trivirgatus). Bovine herpesvirus type 4 (BHV-4) is frequently isolated from diseased and even healthy cattle and occasionally from sheep, wild ruminants and cats. The two viruses are related antigenically, as was revealed by an indirect fluorescent antibody test using polyclonal antisera from experimentally infected rabbits or monoclonal antibodies raised against six BHV-4 proteins, three of which were glycosylated. The genome structures of the two viruses consist of a unique central sequence flanked at both ends by G + C-rich tandem repeats. Restriction maps (produced using EcoRI, BamHI and HindIII) of these two viruses were nearly identical but the unique sequence of the HVA-2 genome possessed two additional BamHI sites. Four genomic regions of variable size were detected, two located in the unique part, one in the repetitive part and one in the left junction between the unique and the repeated part of the genome; these slight variations were similar to those observed between various BHV-4 isolates. These results suggest that HVA-2 and BHV-4 belong to the same virus species; HVA-2 could be either a BHV-4 contaminant of owl monkey kidney cell cultures or an isolate from an owl monkey accidentally infected with BHV-4. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailCytological effects of culture media conditioned B16 melanoma cells and 3T3 fibroblasts
Coucke, Paul; Siwek, B.; Gillet, Marie-Claire ULg et al

in Anticancer Research (1991), 11(2), 801-804

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these ... [more ▼]

Cytotoxic soluble fractions (M.W.<1,000) were prepared from media conditioned by mixed cultures of 3T3 fibroblasts and B16 cells. The ultrastructural analyses of cells (B16 or 3T3) treated with these fractions revealed in them mitochondria swelling, blebs, broken membranes and dead cells. [less ▲]

Detailed reference viewed: 8 (2 ULg)
Peer Reviewed
See detailNeuroanatomical Specificity in the Co-Localization of Aromatase and Estrogen Receptors
Balthazart, Jacques ULg; Foidart, Agnès ULg; Surlemont, C. et al

in Journal of Neurobiology (1991), 22(2), 143-57

The relative distributions of aromatase and of estrogen receptors were studied in the brain of the Japanese quail by a double-label immunocytochemical technique. Aromatase immunoreactive cells (ARO-ir ... [more ▼]

The relative distributions of aromatase and of estrogen receptors were studied in the brain of the Japanese quail by a double-label immunocytochemical technique. Aromatase immunoreactive cells (ARO-ir) were found in the medial preoptic nucleus, in the septal region, and in a large cell cluster extending from the dorso-lateral aspect of the ventromedial nucleus of the hypothalamus to the tuber at the level of the nucleus inferioris hypothalami. Immunoreactive estrogen receptors (ER) were also found in each of these brain areas but their distribution was much broader and included larger parts of the preoptic, septal, and tuberal regions. In the ventromedial and tuberal hypothalamus, the majority of the ARO-ir cells (over 75%) also contained immunoreactive ER. By contrast, very few of the ARO-ir cells were double-labeled in the preoptic area and in the septum. More than 80% of the aromatase-containing cells contained no ER in these regions. This suggests that the estrogens, which are formed centrally by aromatization of testosterone, might not exert their biological effects through binding with the classical nuclear ER. The fact that significant amounts of aromatase activity are found in synaptosomes purified by differential centrifugation and that aromatase immunoreactivity is observed at the electron microscope level in synaptic boutons suggests that aromatase might produce estrogens that act at the synaptic level as neurohormones or neuromodulators. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailCytotoxic and mitogenic activities in culture media conditioned by mouse B16 melanoma cells and 3T3 fibroblasts
Siwek, Brigitte; Wauthy, Jacques ULg; Coucke, Paul et al

in Anticancer Research (1991), 11(2), 755-759

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They ... [more ▼]

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They are generally more active on B16 than on fibroblasts. [less ▲]

Detailed reference viewed: 10 (1 ULg)
See detailProjets d'emploi et lieux d'apprentissage privilégiés d'étudiants en fin de scolarité secondaire
Robert, Jocelyne ULg

in Recherche en Education. Théorie & Pratique (1991), 4

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailEnhancement of 1,3-bis(2-chloroethyl)-1-nitrosourea resistance by gy gamma-irradiation or drug pretreatment in rat hepatoma cells
Habraken, Yvette ULg; Laval, Françoise

in Cancer Research (1991), 51(4), 499-503

Detailed reference viewed: 1 (0 ULg)
Full Text
Peer Reviewed
See detailNucleotide Sequence of the Lipase Gene Lip3 from the Antarctic Psychotroph Moraxella Ta144
Feller, Georges ULg; Thiry, M.; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1991), 1088(2), 323-4

A lipase gene (lip3) from the psychotrophic strain Moraxella TA144 has been cloned and sequenced. The deduced primary structure of the lipase preprotein is composed of 315 amino acids with a predicted Mr ... [more ▼]

A lipase gene (lip3) from the psychotrophic strain Moraxella TA144 has been cloned and sequenced. The deduced primary structure of the lipase preprotein is composed of 315 amino acids with a predicted Mr of 34,772. This enzyme contains two consensus peptides showing cluster of glycine residues that may be involved in domain flexibility. The cloned gene product conserves the low temperature activity and the thermolability properties of the wild enzyme. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailRagged N-Termini and Other Variants of Class a Beta-Lactamases Analysed by Chromatofocusing
Matagne, André ULg; Joris, Bernard ULg; Van Beeumen, J. et al

in Biochemical Journal (1991), 273(273), 503-10

Four beta-lactamases excreted by Gram-positive bacteria exhibited microheterogeneity when analysed by chromatofocusing or ion-exchange chromatography. Ragged N-termini were in part responsible for the ... [more ▼]

Four beta-lactamases excreted by Gram-positive bacteria exhibited microheterogeneity when analysed by chromatofocusing or ion-exchange chromatography. Ragged N-termini were in part responsible for the charge variants, but deamidation of an asparagine residue was also involved, at least for the Bacillus licheniformis enzyme. The activity of a contaminating proteinase could also be demonstrated in the case of Actinomadura R39 beta-lactamase. With that enzyme, proteolysis resulted in partial inactivation, but the inactivated fragments were easily separated from the active forms. With these, as with the other enzymes, the kinetic parameters of the major variants were identical with those of the mixture within the limits of experimental error, so that the catalytic properties of these enzymes can be determined with the 'heterogeneous' preparations. [less ▲]

Detailed reference viewed: 6 (3 ULg)
Full Text
Peer Reviewed
See detailThyrotropin-Secreting Pituitary Adenomas: Report of Seven Cases
Beckers, Albert ULg; Abs, R.; Mahler, C. et al

in Journal of Clinical Endocrinology and Metabolism (1991), 72(2), 477-83

Seven patients with hyperthyroidism due to a TSH-secreting pituitary macroadenoma have been observed of a total of 800 patients with pituitary tumors over a period of 15 yr. Serum TSH levels varied ... [more ▼]

Seven patients with hyperthyroidism due to a TSH-secreting pituitary macroadenoma have been observed of a total of 800 patients with pituitary tumors over a period of 15 yr. Serum TSH levels varied between 1.1-36.3 mU/L. The serum alpha-subunit level was low in 1 case, while in 4 other cases the concentration was elevated and varied between 3.7-7.8 micrograms/L. Serum TSH beta levels were normal in the 4 cases in which it was determined. Serum GH or PRL levels were elevated in 5 cases. In 1 patient the cosecretion of TSH, GH, and PRL was confirmed by immunocytochemical examination. Serum TSH and alpha-subunit responses to TRH, GnRH, CRF, GRF, dexamethasone, methimazole, T3, and bromocriptine administration were variable when studied. Serum TSH and alpha-subunit circadian rhythms were absent in 1 case and inverted in another. A serum alpha-subunit pulsatility without TSH pulses was observed in 1 patient. Five patients underwent transsphenoidal adenomectomy. Three of 4 patients operated on in our center were cured, but a recurrence of the adenoma was found in 1 of them after 5 yr. The fifth patient was not cured. Treatment with octreotide in 3 patients resulted in normalization of serum TSH, GH, and thyroid hormones levels. Cosecretion of PRL in 1 case and alpha-subunit in 2 cases was also inhibited. Partial tachyphylaxis occurred in 1 patient. In summary, heterogeneity in clinical presentation, hormonal expression, and therapeutic response appears to characterize these TSH-secreting adenomas. [less ▲]

Detailed reference viewed: 59 (0 ULg)
Peer Reviewed
See detailPrenatal Diagnosis of Cystic Hygroma and Chorioangioma in the Wolf-Hirschhorn Syndrome
Verloes, Alain ULg; Schaaps, Jean-Pierre ULg; Herens, Christian ULg et al

in Prenatal Diagnosis (1991), 11(2), 129-32

We report the prenatal diagnosis of Wolf-Hirschhorn syndrome (4p-) in a 24-week-old fetus. Echographic features included cystic hygroma, a complex heart defect with right ventricular hypoplasia, and a ... [more ▼]

We report the prenatal diagnosis of Wolf-Hirschhorn syndrome (4p-) in a 24-week-old fetus. Echographic features included cystic hygroma, a complex heart defect with right ventricular hypoplasia, and a large placental chorioangioma. We suggest that chorioangioma may be associated with chromosomal imbalance and that systematic careful morphologic examination of the fetus and karyotyping of any pregnancy in which large chorioangioma is detected is advisable. Jugular lymphatic obstruction sequence has not been reported so far in association with 4p-syndrome. [less ▲]

Detailed reference viewed: 11 (3 ULg)
Peer Reviewed
See detailL'adénome prostatique et son traitement en 1990
Bonnet, Pierre ULg; De Leval, Jean ULg

in Revue Médicale de Liège (1991), 46(2), 45-57

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailMédicaments anti-asthmatiques. De la pharmacologie à la clinique
Louis, Renaud ULg; Radermecker, Maurice ULg

in Revue Médicale de Liège (1991), 46(2), 58-81

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailComparative Morphometric Study of Eruptive Puva-Induced and Chronic Sun-Induced Lentigines of the Skin
Nikkels, Arjen ULg; Ben Mosbah, T.; Pierard-Franchimont, Claudine ULg et al

in Analytical and Quantitative Cytology and Histology (1991), 13(1), 23-26

Computerized image analysis was used to compare lentigines (brown freckle-like cutaneous spots) induced by treatment with psoralens and ultraviolet light A (PUVA-induced lentigines) with those induced by ... [more ▼]

Computerized image analysis was used to compare lentigines (brown freckle-like cutaneous spots) induced by treatment with psoralens and ultraviolet light A (PUVA-induced lentigines) with those induced by solar exposure (actinic lentigines). For these conditions, which appear to be distinctive clinically and histologically, the number, size and shape of the macules were analyzed, revealing significant differences in the two latter parameters. This indicates that UV irradiations of different wave lengths act differently on melanocytes and/or on the interrelation between melanocytes and keratinocytes. The overall effect is much more self-limited in PUVA lentigines than in actinic lentigines, suggesting that the pathophysiology of these lesions is probably different. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailAnalyse qualitative de la modification de comportements d’enseignement par la technique de la ligne de base multiple
Cloes, Marc ULg; Piéron, Maurice ULg

in STAPS : Revue Internationale des Sciences du Sport et de l'Education Physique (1991), 24

Multiple baseline technique has been frequently related to success in teacher training. In this study, it was applied to change three enthusiastic behaviors by a pre-service teacher (hustles, dynamism ... [more ▼]

Multiple baseline technique has been frequently related to success in teacher training. In this study, it was applied to change three enthusiastic behaviors by a pre-service teacher (hustles, dynamism, feedback). The experimental design was planned for 20 weeks. It comprised 28 lessons and was divided into several phases (baseline, treatment, maintenance, follow up). The experimental design was successful as well in target behavior modification as in short - and long-terme stability of the changes. Qualitative analysis of the results provided useful information about the evolution of target behaviors. The procedure pointed out: (1) the specificity of the evolution of the behavioral changes; (2) the influence of various factors on the short term stability of the behavioral changes; (3) the influence of the duration of the treatment on the long term stability of the behavioral changes, and (4) the positive effects of a timely recall intervention. Data confirmed the reinforcing effect of a systematic follow up in teacher prepatation. [less ▲]

Detailed reference viewed: 56 (4 ULg)
Peer Reviewed
See detailQuantitative M-Mode and Two-Dimensional Echocardiography in Calves
Amory, Hélène ULg; Jakovljevic, S.; Lekeux, Pierre ULg

in Veterinary Record : Journal of the British Veterinary Association (1991), 128(2), 25-31

A standardised echocardiographic protocol was applied to 18 healthy calves for the estimation of 51 anatomical and functional cardiac variables. These variables were measured in a long axis and a short ... [more ▼]

A standardised echocardiographic protocol was applied to 18 healthy calves for the estimation of 51 anatomical and functional cardiac variables. These variables were measured in a long axis and a short axis view of the heart, and both two-dimensional and cursor-directed time-motion (M-) modes were used. The repeatability of each of the measurements was estimated by comparing values obtained twice within 24 hours, and most of the 51 variables showed a high degree of repeatability. Statistical analysis revealed a close correlation between cardiac measurements taken post mortem and by in vivo echocardiography, and between the echocardiographic values obtained in different scanning planes of the heart or ultrasonic modes, indicating that quantitative echocardiography is a reliable technique in calves. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailNebulae around R Coronae Borealis stars - Clues to their evolutionary status?
Pollacco, D. L.; Hill, P. W.; Houziaux, Léo ULg et al

in Monthly Notices of the Royal Astronomical Society (1991), 248

Imaging and spectroscopy are presented of the classical R CrB star UW Cen obtained when the star was in a deep minimum. These show the presence of a faint reflection nebula, the inner parts of which ... [more ▼]

Imaging and spectroscopy are presented of the classical R CrB star UW Cen obtained when the star was in a deep minimum. These show the presence of a faint reflection nebula, the inner parts of which display two sets of symmetrical 'jets' centered on the star. The possibilities of whether the nebular structure can be associated with last thermal pulse evolution, white dwarf merger, or the mechanism causing the characteristic R CrB minima are discussed. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailSecond supplement to the catalogue of observed periods of Ap stars
Catalano, F. A.; Renson, Pierre ULg; Leone, F.

in Astronomy and Astrophysics. Supplement Series (1991), 87

Supplementary data on the periods of Ap stars with references are presented 20 new stars are introduced for which a periodic variability has been discovered since 1987 or it was known before but it was ... [more ▼]

Supplementary data on the periods of Ap stars with references are presented 20 new stars are introduced for which a periodic variability has been discovered since 1987 or it was known before but it was not reported in the previous issues of this catalogue. For many of the stars in the previous catalogues new determinations of the period are introduced. Recently attributed variable star names are also reported. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailInvasion of Reconstituted Basement Membrane Matrix Is Not Correlated to the Malignant Metastatic Cell Phenotype
Noël, Agnès ULg; Calle, A.; Emonard, H. et al

in Cancer Research (1991), 51(1), 405-14

Interactions between tumor cells and basement membranes represent a critical step in the progression of neoplasia and in the metastatic process. Reconstituted basement membrane matrix, matrigel, has been ... [more ▼]

Interactions between tumor cells and basement membranes represent a critical step in the progression of neoplasia and in the metastatic process. Reconstituted basement membrane matrix, matrigel, has been recently used with the aim of developing an in vitro assay of tumor cell invasiveness. We have extended these studies by comparing the invasiveness of a large series of normal and malignant epithelial and mesenchymal cells of human and animal origin cultured on matrigel. Normal cells (fibroblasts, glomerular mesangial cells, keratinocytes), human fibrosarcoma cells (HT1080), and reticular sarcoma cells (M5076) clearly established invasive capabilities in the matrix. However, all the other tested cell lines, malignant or virally transformed cells invasive in vivo (MCF7, T47D, SA52, SW613, MO4, A431, BeWo), as well as normal nontransformed cells (MOH22) were incapable of penetration. The morphological features of matrigel invasion by normal fibroblasts and HT1080 cells are described at the light and electron microscope levels. The extent of degradation of a radiolabeled matrigel is minimal and similar in several cell lines reported to be noninvasive or invasive in vivo. Our data suggest that matrigel does not provide a universal model to correlate the invasiveness of cells in vivo and in vitro. [less ▲]

Detailed reference viewed: 8 (3 ULg)
Peer Reviewed
See detailSous-groupes libres dans les groupes d’automorphismes d’arbres
Pays, Isabelle ULg; Valette, Alain

in L'enseignement mathématique (1991), 37

Detailed reference viewed: 54 (0 ULg)
Full Text
Peer Reviewed
See detailDistribution of Aromatase-Immunoreactive Cells in the Mouse Forebrain
Balthazart, Jacques ULg; Foidart, Agnès ULg; Surlemont, C. et al

in Cell & Tissue Research (1991), 263(1), 71-9

The distribution of aromatase-immunoreactive cells was studied by immunocytochemistry in the mouse forebrain using a purified polyclonal antibody raised against human placental aromatase. Labeled ... [more ▼]

The distribution of aromatase-immunoreactive cells was studied by immunocytochemistry in the mouse forebrain using a purified polyclonal antibody raised against human placental aromatase. Labeled perikarya were found in the dorso-lateral parts of the medial and tuberal hypothalamus. Positive cells filled an area extending between the subincertal nucleus in the dorsal part, the ventromedial hypothalamic nucleus in the ventral part, and the internal capsule and the magnocellular nucleus of the lateral hypothalamus in the lateral part. The same distribution was seen in the two strains of mice that were studied (Jackson and Swiss), and the number of immunoreactive perikarya did not seem to be affected by castration or testosterone treatment. No immunoreactivity could be detected in the medial regions of the preoptic area and hypothalamus; these were expected to contain the enzyme based on assays of aromatase activity performed in rats and on indirect autoradiographic evidence in mice. Our data raise questions concerning the distribution of aromatase in the brain and the mode of action of the centrally produced estrogens. [less ▲]

Detailed reference viewed: 10 (2 ULg)
Peer Reviewed
See detailAspects immunologiques actuels de la maladie de Crohn
Louis, Edouard ULg; Belaiche, Jacques ULg; Gast, Pierrette ULg et al

in Revue Médicale de Liège (1991), 46(1), 2-11

Detailed reference viewed: 22 (4 ULg)
Full Text
Peer Reviewed
See detailMagnetic Stimulation of the Motor Cortex in Cervical Spondylosis
Maertens De Noordhout, Alain ULg; Remacle, J. M.; PEPIN, Jean-Louis ULg et al

in Neurology (1991), 41(1), 75-80

We report a new technique of transcranial magnetic stimulation of the motor cortex to measure conduction within central motor pathways of 67 patients with cervical spondylosis or disk herniation. There ... [more ▼]

We report a new technique of transcranial magnetic stimulation of the motor cortex to measure conduction within central motor pathways of 67 patients with cervical spondylosis or disk herniation. There were upper motor neuron signs in 34 patients (51%) and x-ray evidence of cervical cord compression in 44 (66%). Muscle action potentials (MAPs) to cortical stimulation were abnormal in 84% of patients with, and 22% of those without, radiologic signs of cervical cord compression. Median nerve somatosensory evoked potentials were altered in only 25% of patients. The frequency of MAP alterations correlated with upper motor neuron signs. In 5 (11%) of the 44 patients with x-ray evidence of cervical cord compression, subclinical cord compression was disclosed by cortical stimulation. In 10 patients restudied 3 months after surgical decompression, normalization of central motor conduction time did not occur, indicating permanent damage to the cervical cord. [less ▲]

Detailed reference viewed: 5 (3 ULg)
Peer Reviewed
See detailTraitement immunosuppresseur et maladie de Crohn
Delwaide, Jean ULg; Louis, Edouard ULg; Gast, Pierrette ULg et al

in Revue Médicale de Liège (1991), 46(1), 22-8

Detailed reference viewed: 41 (1 ULg)
Peer Reviewed
See detailLes indices d'activité dans la maladie de Crohn
Belaiche, Jacques ULg; Louis, Edouard ULg; Delwaide, Jean ULg et al

in Revue Médicale de Liège (1991), 46(1), 11-5

Detailed reference viewed: 19 (2 ULg)
Peer Reviewed
See detailLes nouveaux dérivés de la salazosulfapyridine dans le traitement de la maladie de Crohn
Belaiche, Jacques ULg; Delwaide, Jean ULg; Louis, Edouard ULg et al

in Revue Médicale de Liège (1991), 46(1), 16-22

Detailed reference viewed: 50 (1 ULg)
Peer Reviewed
See detailNotes sur la dégradabilité dans le rumen de 6 aliments riches en matières grasses
Dufrasne, Isabelle ULg; Istasse, Louis ULg; Van Eenaeme, Christian ULg et al

in Revue de l’Agriculture = Landbouwtijdschrift (1991), 44(1), 31-38

Detailed reference viewed: 26 (7 ULg)
Peer Reviewed
See detailVector mesons and partial restoration of chiral symmetry
Mendez Galain, Ramon; Ripka, Georges; Jaminon, Martine ULg et al

in Europhysics Letters [=EPL] (1991), 14

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailThe glucocorticoid receptor.
Muller, Marc ULg; Renkawitz, R.

in Biochimica et Biophysica Acta (1991), 1088

Detailed reference viewed: 9 (1 ULg)
See detailL'intérêt porté par les poètes et les traducteurs russes à l'Ode III, 30 d'Horace (Exegi monumentum)
Mouton, Charles ULg

in Latomus : Revue d'Etudes Latines (1991), L(3), 654-670

Detailed reference viewed: 10 (2 ULg)
Peer Reviewed
See detailDiversity of the Mechanisms of Resistance to Beta-Lactam Antibiotics
Frère, Jean-Marie ULg; Joris, Bernard ULg; Granier, B. et al

in Research in Microbiology (1991), 142(6, Jul-Aug), 705-10

The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and ... [more ▼]

The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and properties of the beta-lactamase(s) and the diffusion barrier that the outer-membrane of Gram-negative bacteria can represent. Those three factors can be modified by mutations or by the horizontal transfer of genes or portions of genes. [less ▲]

Detailed reference viewed: 41 (3 ULg)
Peer Reviewed
See detailYohimbine can induce ethanol tolerance in an in vitro preparation of rat locus coeruleus.
Verbanck, P.; Seutin, Vincent ULg; Massotte, Laurent ULg et al

in Alcoholism, Clinical & Experimental Research (1991), 15(6), 1036-9

Noradrenergic neurons have been implicated in the development of ethanol dependence and tolerance. Moreover, the development of an hyposensitivity of alpha 2 adrenoceptors has been postulated during long ... [more ▼]

Noradrenergic neurons have been implicated in the development of ethanol dependence and tolerance. Moreover, the development of an hyposensitivity of alpha 2 adrenoceptors has been postulated during long-term exposition to ethanol. In order to test the putative role of alpha 2 receptors in ethanol intoxication, we have studied the interaction between ethanol and yohimbine, an alpha 2 antagonist, on the spontaneous firing rate of rat locus coeruleus (LC) in an in vitro slice model. The spikes from single neurons were recorded by glass microelectrodes. Ethanol at 100 mM, a concentration that parallels the behavioral effects in the human and in the animals, inhibits the firing activity of some LC cells. This inhibition was quickly reversed after stopping the ethanol perfusion and was observed for each further administration. However, if yohimbine (20 microM) was simultaneously perfused, the ethanol-induced inhibition was rapidly antagonized. This effect is reversible after long time washout of yohimbine. This suggests that alpha 2 adrenoceptors could be implicated in the inhibitory effect of ethanol on LC noradrenergic neurons and perhaps in the development of tolerance. However, other hypotheses are discussed, because yohimbine can also antagonize other types of receptors. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailVertical Column Abundances and Seasonal Cycle of Acetylene, C2H2, above the Jungfraujoch Station, Derived from IR Solar observations
Zander, Rodolphe ULg; Rinsland, C.P.; Ehhalt, D.H. et al

in Journal of Atmospheric Chemistry (1991), 13

Monthly mean total vertical column abundances of acetylene have been determined from series of infrared solar spectra recorded at the Jungfraujoch station, Switzerland, between June 1986 and April 1991 ... [more ▼]

Monthly mean total vertical column abundances of acetylene have been determined from series of infrared solar spectra recorded at the Jungfraujoch station, Switzerland, between June 1986 and April 1991. The data have been obtained by nonlinear least-squares fittings of the v5 band R19 transition of C2H2 at 776.0818 cm-1. The average of 22 monthly mean total vertical columns of C2H2 retrieved during that time interval of almost 5 years was found to be equal to (1.81 +- O.12) X 1OE15 molec/cm2, which corresponds to an average mixing ratio of (0.22 +- O.013) ppbv (parts per billion by volume) in a troposphere extending from the altitude of the station (3.58 km), up to 10.5 km. Despite the large variability found from year to year, a least-squares sine fit to the data reveals a seasonal variation with an amplitude of about +- 40 % of the mean; the maximum occurs during mid-winter and the minimum in the summer. The present results are compared critically with similar in-situ data found in the literature. A sinusoidal fit to ail such free troposphere measurements made in-situ between 30° N and 60° N indicates good agreement in shape and phase with the seasonal variation derived above the Jungfraujoch, but their average column abundance, 2.3 X lOE15 molec/cm2, is about 30 % higher; this difference is explained on the basis of non-upwelling meteorological conditions generally prevailing during ground-based remote solar observations. [less ▲]

Detailed reference viewed: 8 (2 ULg)
Full Text
See detailObservations on seed protein electrophoretic patterns of some species within the genus Phaseolus.
Baudoin, Jean-Pierre ULg; Schmit, V.; Wathelet, Bernard ULg

in Annual Report of the Bean Improvment Cooperative (1991), 34

Detailed reference viewed: 4 (1 ULg)
Peer Reviewed
See detailMICROSCOPIC RHEOLOGICAL MODELS AND EXTENDED IRREVERSIBLE THERMODYNAMICS
Dauby, Pierre ULg; Lebon, Georgy ULg

in Applied Mathematics Letters (1991), 4(6), 39-43

Detailed reference viewed: 3 (0 ULg)
See detailLa recherche en Brucellose entreprise par le CEB (1e partie)
Limet, J.; Oanh Vo, T. K.; Saegerman, Claude ULg et al

in Les élevages belges (1991)

Detailed reference viewed: 7 (1 ULg)
Peer Reviewed
See detailStructure and Beneficiation of a rich gold ore
Ek, Corneille; Pirard, Eric ULg; Baguette, Luc

in ATB Métallurgie (1991), 1-2

Detailed reference viewed: 41 (6 ULg)
Full Text
See detailEchantillonnage à probabilité de sélection proportionnelle à la prédiction et inventaires forestiers.
Hebert, Jacques ULg; Rondeux, Jacques ULg

in Annales de Gembloux (1991), 97(3), 221-231

The so-called 3 P sampling system (sampling with Probability Proportional to Prediction) is a variation of list sampling that does not require a list of population elements prior to the inventory. Every ... [more ▼]

The so-called 3 P sampling system (sampling with Probability Proportional to Prediction) is a variation of list sampling that does not require a list of population elements prior to the inventory. Every tree in a timber sale has to be visited and one needs an estimate of the total volume. In order to correct the total estimated volume, a small number of sample trees are selected according to a unequal-probability selection rule. The predicted tree volume is paired with a random number. Trees with predicted values larger than or equal to the random number are precisely measured. The average ratio of the measured volume to the estimated volume in the sample is used to adjust the total estimated volume. Such a system of inventory is often designed where a highly precise estimate of the volume or value of standing trees is required. [less ▲]

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailInfluence of Electronic Excitation in the Charge Reversal of halogenated Cations.
Leyh, Bernard ULg; Wankenne, H.

in International Journal of Mass Spectrometry and Ion Processes (1991), 107

Charge reversal of high kinetic energy (8keV) cations during collisions with atomic target gases (G=Xe, Kr, Ne) has been studied for CCln+ (n=1 or 2) ions resulting from dissociative electroionization of ... [more ▼]

Charge reversal of high kinetic energy (8keV) cations during collisions with atomic target gases (G=Xe, Kr, Ne) has been studied for CCln+ (n=1 or 2) ions resulting from dissociative electroionization of CCl4. The influence of electronic excitation of the incident cation CCln+ and of the nature of the target gas G on the anion yield have been examined. In our experiments, the two-collision process CCln+->CCln+G+->(CCln-)+2G+ is predominant. By comparing the anion and cation yields as a function of the kinetic energy of the ionizing electrons, we found evidence, with some target gases, of the existence of long-lived excited states of CCl+ and CCl2+, which lead to an increase in the anion yield in comparison with charge reversal of the correponding ground state ions. For CCl+, we deduce an excitation energy of 4+/-1 eV with a lifetime longer than 6.2 µs. These results are compatible with the a3Pi long-lived state of CCl+ previously detected by optical spectroscopy and by charge-stripping experiments. Our experimental data on charge reversal and metastable dissociation of CCl2+ reveal the existence of a long-lived state (lifetime>8.2 µs) of CCl2+ lying at 4.3+/-1 eV above the ground state.Semi-empirical MNDO calculations show that the energy of the first quartet state, a4A2, is compatible with the observed excitation energy. The spin-orbit coupling-induced transition from the a4A2 state to the B2A2 state is suggested to play an important role in the metestable dissociation CCl2+*->(CCl+)+Cl. The origin of the increase in the anion yield when long-lived excited states of CCl+ and CCl2+ are charge-reversed is discussed. Step a is suggested to be responsible for this yield enhancement which is observed when an appropriate target gas makes this step quasi-resonant. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailLocalization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in Electron Microscopy Reviews (1991), 4(1), 85-110

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well ... [more ▼]

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA topoisomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailIntérêt de l'enfant et paiement des pensions alimentaires après divorce en Belgique
Bawin-Legros, Bernadette; Gauthier, Anne; Guillaume, Jean-François ULg

in Population : Revue Trimestrielle de l'Institut National d'Etudes Démographiques (1991), 4

This paper is a study of parental behaviour, and more particularly that of fathers, after divorce, in which findings from a national survey carried out in Belgium are used. The payment of alimony is ... [more ▼]

This paper is a study of parental behaviour, and more particularly that of fathers, after divorce, in which findings from a national survey carried out in Belgium are used. The payment of alimony is considered to be not solely a financial solution, but as an indication of a certain acceptance of parental responsibility which is rooted in the marriage and family background that existed before the couple separated. Legal practise, which generally gives care and control of the child to the mother, also results in the continuity of the traditional type of the division of labour between the parents after their marriage has broken down. This supports the moral principle of a privileged mother-child relationship, but may also lead to a deterioration in the living standards of the mothers' households. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Peer Reviewed
See detailCytochemical distinction of various nucleolar components in insect cells.
Thiry, Marc ULg; Schoonbroodt, Stéphanie ULg; Goessens, Guy ULg

in Biology of the Cell (1991), 72(1-2), 133-40

The fine structure of the insect Sf9 cell nucleolus has been investigated by means of different cytochemical and immunocytochemical techniques at the electron microscope level. Apart from a few ... [more ▼]

The fine structure of the insect Sf9 cell nucleolus has been investigated by means of different cytochemical and immunocytochemical techniques at the electron microscope level. Apart from a few perinucleolar condensed chromatin clumps, the insect cell nucleolus comprises two compartments. The first of these consists of a roundish compact zone formed of fibrillar material. The other is composed of fibrillar and granular structures organized into a network separated by interstitial spaces. But, unlike mammalian cell nucleoli, any fibrillar center has been observed in the Sf9 cell nucleolus, even after actinomycin D treatment. We also show that the compact fibrillar zone of Sf9 cell nucleoli contains silver-stainable material and DNA. In actinomycin D-treated cells, a preferential contact of this compact fibrillar zone with condensed chromatin has been visualized. Finally, silver-stainable material has been found to persist throughout the whole mitosis. These results suggest that the compact fibrillar zone at the insect Sf9 cell nucleolus should, at least partly, correspond to the fibrillar center of mammalian cell nucleoli. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailTruncal Vagotomy and Pyloroplasty Combined with Valvular Replacement in Patients with Ulcer Disease
Defraigne, Jean-Olivier ULg; Innocenti, C.; DEKOSTER, Guy ULg et al

in Journal of Cardiovascular Surgery (The) (1991), 32(1), 42-5

In 1988, 5 patients (3 men and 2 women) with ulcer disease (mean age 56 +/- 8 years) underwent valvular replacement for aortic (No. = 4) or mitral disease (No. = 1). All patients had had gastroduodenal ... [more ▼]

In 1988, 5 patients (3 men and 2 women) with ulcer disease (mean age 56 +/- 8 years) underwent valvular replacement for aortic (No. = 4) or mitral disease (No. = 1). All patients had had gastroduodenal ulcers. Preoperative gastroscopy demonstrated active ulcers (No. = 4) and a healed pyloric ulcer with pyloric stenosis (No. = 1). Despite the presence of ulcers, a non-biologic prosthesis was preferred in each patient because of their young age (No. = 3), chronic atrial fibrillation requiring anticoagulant therapy (No. = 1), and refusal of the eventuality of subsequent reoperation (No. = 1). In each patient, a truncal vagotomy with pyloroplasty was performed simultaneously with the valvular procedures by the same incision. The postoperative courses were uneventful. With a mean follow-up of 15 +/- 3 months, no gastrointestinal bleeding was observed during anticoagulant therapy. With anticoagulant drugs, bleeding may occur with a frequency of 4% per patient treatment-year, half of which are gastrointestinal in origin. Nevertheless, in selected patients with gastroduodenal ulcers, performing a vagotomy-pyloroplasty simultaneously with valvular replacement allows implantation of a non-biologic prosthesis, with greater durability than bioprosthesis. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailDNase I-sensitive sites within the nuclear architecture visualized by immunoelectron microscopy.
Thiry, Marc ULg

in DNA & Cell Biology (1991), 10(3), 169-80

Nick-translation using mild digestion with DNase I allows preferential labeling of actively transcribing or potentially active genes, as compared with inactive genes. We have adapted this method to the ... [more ▼]

Nick-translation using mild digestion with DNase I allows preferential labeling of actively transcribing or potentially active genes, as compared with inactive genes. We have adapted this method to the level of electron microscopy to see the DNase I-sensitive regions in situ in Ehrlich tumor cells. In interphase cells treated with very low concentrations of DNase I, labeled sequences are found at the borders and in the close vicinity of condensed chromatin blocks. Labeling of condensed areas of chromatin requires higher DNase I concentrations and longer incubation in the nick-translation medium. In the nucleolus, the first sites to be nick-translated are the fibrillar centers and the interstices surrounding them, whereas the dense fibrillar component never contains labeled sequences. When cells are pretreated with actinomycin D, only a few perinucleolar clumps of condensed chromatin are labeled under the same conditions. This method provides a new tool for studying the functional organization of chromatin within a cell. The precise location of nick-translated sites in nucleolar components observed could change classical views concerning the functional organization of the nucleolus. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailLa résistance aux pesticides : mécanismes et prévention
Delaunois, A.; Gustin, Pascal ULg; Ansay, M.

in Annales de Médecine Vétérinaire (1991), 135

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailIn situ nick translation at the electron microscopic level: a tool for studying the location of DNAse I-sensitive regions within the cell.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1991), 39(6), 871-4

The in situ nick translation method was adapted to the ultrastructural level, to study the location of DNAse I-sensitive sequences within the cell. Ultra-thin sections of Lowicryl-embedded cells were ... [more ▼]

The in situ nick translation method was adapted to the ultrastructural level, to study the location of DNAse I-sensitive sequences within the cell. Ultra-thin sections of Lowicryl-embedded cells were incubated in a medium containing DNAse I, DNA polymerase I, and all four deoxyribonucleotides, some being biotinylated. The nick-translated sites were then visualized by an indirect immunogold labeling technique. The resulting labeling pattern is closely dependent on the DNAse I concentration in the nick-translation medium. The method reveals with great precision the specific DNAse I-sensitive regions within the nucleus. This technique can be used to discriminate between active and inactive regions of interphase chromatin. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailPotentialités de l'application de l'oxymétrie à infra-rouge basée sur la pulsation dans l'espèce canine
Lemaire, M.; Art, Tatiana ULg; Henroteaux, Marc ULg et al

in Annales de Médecine Vétérinaire (1991), 135

Detailed reference viewed: 11 (2 ULg)
Peer Reviewed
See detailA Monoclonal Elisa for Bovine Viral Diarrhoea Pestivirus Antigen Detection in Persistently Infected Cattle
Mignon, Bernard ULg; Dubuisson, J.; Baranowski, E. et al

in Journal of Virological Methods (1991), 35(2), 177-188

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal ... [more ▼]

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailReproductive Development in Lolium temulentum L.: Spike Morphogenesis and Grain Set Limitations
Périlleux, Claire ULg; Bernier, Georges ULg; Kinet, Jean Marie

in Journal of Experimental Botany (1991), 42(4), 501

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was ... [more ▼]

Reproductive development in cereals is not easy to investigate because their quantitative response to environmental factors makes it difficult to synchronize the plants. In this paper, one of our aims was to assess whether Lolium temulentum strain Ceres, a qualitative long-day grass, could serve as a model of reproductive development for cereals. The morphological patterns from floral transition to seed set were studied. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailItaly : two Perspectives. Racism in Paradise ?
Martiniello, Marco ULg; Kazim, P.

in Race & Class (1991), 32(3), 79-89

Detailed reference viewed: 19 (1 ULg)
Full Text
See detailErythropoïétine : utilisation diagnostique et thérapeutique
Beguin, Yves ULg; Fillet, Georges ULg

in Revue de l’Association Belge des Technologues de Laboratoire = Tijdschrift van de Belgische Vereniging van Laboratoriumtechnologen (1991), 18(3), 161-168

L'érythropoïétine est une hormone produite par le rein en réponse à une hypoxie rénale et qui stimule l'activité érythropoïétique de la moelle osseuse. Le dosage de l'érythropoïétine sérique, toujours ... [more ▼]

L'érythropoïétine est une hormone produite par le rein en réponse à une hypoxie rénale et qui stimule l'activité érythropoïétique de la moelle osseuse. Le dosage de l'érythropoïétine sérique, toujours interprété en fonction de l'hématocrite circulant, est utile dans le diagnostic différentiel des polyglobulies et dans certains cas particuliers d'anémie. L'utilisation thérapeutique de l'érythropoïétine recombinante a permis de corriger l'anémie de l'insuffisance rénale chronique et est actuellement étudiée dans d'autres indications telles que les anémies inflammatoires. [less ▲]

Detailed reference viewed: 40 (4 ULg)
Peer Reviewed
See detailEtude sur les fluctuations de température relevées dans 20 compartiments de maternité en porcherie d'élevage
Debliquy, P.; Nicks, Baudouin ULg; Canart, Bernard et al

in Annales de Médecine Vétérinaire (1991)

Detailed reference viewed: 5 (0 ULg)
See detailLa rentabilite du triticale
Cors, François; Bodson, Bernard ULg; Falisse, André ULg

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(4), 519-535

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailImpact de la pollution atmosphérique sur le système respiratoire. I : l’ammoniac
Gustin, Pascal ULg; Urbain, B.; Ansay, M. et al

in Annales de Médecine Vétérinaire (1991), 135

Detailed reference viewed: 34 (2 ULg)
Peer Reviewed
See detailWhat's in a name ? Access to information from people's names
Valentine, T.; Brédart, Serge ULg; Lawson, R. et al

in European Journal of Cognitive Psychology (1991), 3(1), 147-176

Detailed reference viewed: 52 (5 ULg)
See detailLes "Villes-santé" de l'O.M.S
Gosset, Christiane ULg

in Education Santé (1991)

Detailed reference viewed: 17 (5 ULg)
Full Text
Peer Reviewed
See detailMolecular size and symmetry of Pseudomonas aeruginosa catabolic ornithine carbamoyltransferase. An X-ray crystallography analysis.
Marcq, S.; Diaz-Ruano, A.; Charlier, Paulette ULg et al

in Journal of Molecular Biology (1991), 220(1), 9-12

The catabolic ornithine carbamoyltransferase (EC 2.1.3.3) from Pseudomonas aeruginosa, that shows allosteric behaviour, and a mutant version of this enzyme has been crystallized in several different ... [more ▼]

The catabolic ornithine carbamoyltransferase (EC 2.1.3.3) from Pseudomonas aeruginosa, that shows allosteric behaviour, and a mutant version of this enzyme has been crystallized in several different crystal forms. All of these have been characterized by X-ray diffraction methods. A 4.5 A resolution data set has been collected on a triclinic crystal. Analysis of the data using the self-rotation function shows that 12 monomers associate to form a particle with cubic 23 point group symmetry. [less ▲]

Detailed reference viewed: 6 (1 ULg)