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See detailMolecular and pigment studies of the picophytoplankton in a region of the Southern Ocean (42-54 degrees S, 141-144 degrees E) in March 1998
Wilmotte, Annick ULg; Demonceau, Caroline; Goffart, Anne ULg et al

in Deep-Sea Research Part II, Topical Studies in Oceanography (2002), 49(16), 3351-3363

Seven filtered seawater samples (depths between 30 and 55 m) collected during the SAZ project of the Austral summer of 1997-1998 were used for a simultaneous study of the picophytoplankton pigments based ... [more ▼]

Seven filtered seawater samples (depths between 30 and 55 m) collected during the SAZ project of the Austral summer of 1997-1998 were used for a simultaneous study of the picophytoplankton pigments based on high-performance liquid chromatography (HPLC) analyses and flow cytometry, and of the molecular diversity of the picophytoplankton based on their rDNA sequences. The sampling sites could be divided into three temperature zones, distinguished by their proximity to the Sub-Antarctic and Polar Fronts. HPLC analysis of total chlorophylls and carotenoids showed fairly low phytoplankton concentrations (77-262 ng chl al(-1)), with minimal values of the pigments in the two samples of the Polar Front Zone around 54degreesS (water temperature of 4degreesC at time of collection). In this zone, a similar decrease of particles, identified as cyanobacteria on the basis of their fluorescence, was observed by flow cytometry. Sequences very similar to the 16S rDNA sequence of Synechococcus WH8103 were present in all samples. This Synechococcus genotype is thus found in the Southern Ocean in addition to the Atlantic and Pacific locations where it has been previously observed. The yield of PCR products was lower in the two samples taken in the Polar Front Zone, showing a good agreement between molecular and pigment data. 16S rDNA sequences of plastids of eukaryotic algae also were retrieved, mostly related to those of an environmental clone called OM164, which has not been cultivated but has phylogenetic affinities to the Raphidophyceae. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailPositron emission tomography (PET) evaluation of abdominal aortic aneurysm (AAA)
SakalihasanN, Natzi ULg; Van Damme, Hendrik ULg; Gomez, P. et al

in European Journal of Vascular and Endovascular Surgery (2002), 23(5), 431-436

Background: aneurysmal disease is associated with all inflammatory Cell infiltrate and enzymatic degradation of the vessel wall. Aim of the study: to detect increased metabolic activity in abdominal ... [more ▼]

Background: aneurysmal disease is associated with all inflammatory Cell infiltrate and enzymatic degradation of the vessel wall. Aim of the study: to detect increased metabolic activity in abdominal aortic aneurysms (AAA) by means of positron emission tomography (PET-imaging). Study design: twenty-six patients with AAA underwent PET-imaging Results: in tell patients, PET-imaging revealed increased, fluoro-deoxy-glucose (18-FDG) uptake at the level of the aneurysm. Patients with positive PET-imaging had one or more of the following elements in their clinical history: history Of recent non-aortic surgery (n = 4) a painful inflammatory aortic aneurysm (n = 2). moderate low back pain (n = 2), rapid (>5 mm in 6 months) expansion (n = 4), discovery by PET-scan of a previously undiagnosed lung cancer (n = 3) or parotid tumour (n = 1). Five patients with a positive PET scan required urgent surgery within two to 30 days. Among the 16 patients with negative PET-imaging of their aneurysm, only one had recent non-aortic surgery, none of them required urgent surgery, only two had a rapidly expanding AAA, and in only one patient, PET-imaging revealed an unknown lung cancer. Conclusion: these data suggest a possible association between increased 18-FDG uptake and AAA expansion and rupture. [less ▲]

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See detailSyndrome cérébelleux paradoxal provoqué par un méningiome chez une vache
Danlois, F.; Poncelet, Luc; Rollin, Frédéric ULg

in Annales de Médecine Vétérinaire (2002), 146

A paradoxical vestibular syndrome caused by a fibroblastic meningioma is described in a 20 month-old Holstein heifer. Clinical signs included head tilt, jerking movements of the head and neck, circling to ... [more ▼]

A paradoxical vestibular syndrome caused by a fibroblastic meningioma is described in a 20 month-old Holstein heifer. Clinical signs included head tilt, jerking movements of the head and neck, circling to the left, and also abducted and hypermetric right limbs. The animal was culled and the forebrain therefore was unavailable for histopathological study. The tumour was found on the right side, adherent to the dura mater at the level of the pontomedullary junction. The paradoxical vestibular signs were explained by the presence of the mass causing a loss of cerebellar inhibition over homolateral vestibular nuclei resulting in hyperactivity on the same side as the tumour and simulating a contralateral functional loss. The jerking head and neck movements might be caused by the same lesion although a concurrent forebrain lesion could not be excluded. [less ▲]

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See detailLes acteurs et les enjeux au coeur de l'évaluation
Vandoorne, Chantal ULg

in Education santé (2002), 173(173), 9-11

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See detailComparison of the collision-induced dissociation of duplex DNA at different collision regimes: Evidence for a multistep dissociation mechanism
Gabelica, Valérie ULg; De Pauw, Edwin ULg

in Journal of the American Society for Mass Spectrometry (2002), 13(1), 91-98

The dissociation mechanism of duplex DNA has been investigated in detail by collision-induced dissociation experiments at different collision regimes. MS/MS experiments were performed either in a ... [more ▼]

The dissociation mechanism of duplex DNA has been investigated in detail by collision-induced dissociation experiments at different collision regimes. MS/MS experiments were performed either in a quadrupole collision cell (hybrid quadrupole-TOF instrument) or in a quadrupole ion trap with different activation times and energies. In addition to the noncovalent dissociation of the duplex into the single strands, other covalent bond fragmentation channels were observed. Neutral base loss from the duplex is favored by slow activation. In fast activation conditions, however, the major reaction channel is the noncovalent dissociation into single strands, which is highly entropy-favored. Fast activation regimes can favor the entropy-driven noncovalent dissociation, while in slow heating conditions the competition with enthalpy-driven covalent fragmentation can completely hinder the dissociation of the complex. We also evidence that the noncovalent dissociation of DNA duplex is a multistep process involving a progressive unzipping, preferentially at terminal positions. This is proposed to be a general feature for complexes containing a high number of contributing interactions organized at the interface of the ligands. The overall (observed) dissociation kinetics of noncovalent complexes can therefore depend on a complicated mechanism for which a single transition state description of the kinetics is too simplistic. [less ▲]

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See detailHeat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice.
McMillan, D Randy; Christians, Elisabeth; Forster, Michael et al

in Molecular & Cellular Biology (2002), 22(22), 8005-14

Members of the heat shock factor (HSF) family are evolutionarily conserved regulators that share a highly homologous DNA-binding domain. In mammals, HSF1 is the main factor controlling the stress ... [more ▼]

Members of the heat shock factor (HSF) family are evolutionarily conserved regulators that share a highly homologous DNA-binding domain. In mammals, HSF1 is the main factor controlling the stress-inducible expression of Hsp genes while the functions of HSF2 and HSF4 are less clear. Based on its developmental profile of expression, it was hypothesized that HSF2 may play an essential role in brain and heart development, spermatogenesis, and erythroid differentiation. To directly assess this hypothesis and better understand the underlying mechanisms that require HSF2, we generated Hsf2 knockout mice. Here, we report that Hsf2(-/-) mice are viable and fertile and exhibit normal life span and behavioral functions. We conclude that HSF2, most probably because its physiological roles are integrated into a redundant network of gene regulation and function, is dispensable for normal development, fertility, and postnatal psychomotor function. [less ▲]

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See detailSeparation, identification and quantitation of ceramides in human cancer cells by liquid chromatography–electrospray ionization tandem mass spectrometry
Fillet, Marianne ULg; Van Heugen, Jean-Claude ULg; Servais, Anne-Catherine ULg et al

in Journal of Chromatography. A (2002), 949

Ceramides are important intracellular second messengers that play a role in the regulation of cell growth, differentiation and programmed cell death. Qualitative and quantitative analysis of these second ... [more ▼]

Ceramides are important intracellular second messengers that play a role in the regulation of cell growth, differentiation and programmed cell death. Qualitative and quantitative analysis of these second messengers requires sensitive and specific analytical methods to detect endogenous levels of individual ceramide species and to differentiate between them. Nine synthetic ceramides were separated by liquid chromatography coupled to tandem mass spectrometry on a C bonded silica 18 column. The lipids were eluted in gradient elution mode using a mixture of water, acetonitrile and 2-propanol as mobile phase. They were detected by reaction monitoring performed on positive ion electrospray generated ions. Collision-induced fragmentations conducted on ceramides produced a well characteristic product ion at m/z 264, making multiple reaction monitoring (MRM) well suited for various ceramides quantitative measurements. After optimization of the extraction step, the proposed methodology was able to identify and quantify different ceramide species issued from human cancer cells. The method could be validated for C , C and C ceramides, quantified at the nanogram level. The validation exhibits good 16 18 20 results with respect to linearity, accuracy and precision. [less ▲]

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See detailLipid-Interacting Properties Of The N-Terminal Domain Of Human Apolipoprotein C-III
Lins, Laurence ULg; Flore, Christelle ULg; Chapelle, L. et al

in Protein Engineering (2002), 15(6), 513-20

The lipid-interacting properties of the N-terminal domain of human apolipoprotein C-III (apo C-III) were investigated. By molecular modeling, we predicted that the 6-20 fragment of apo C-III is obliquely ... [more ▼]

The lipid-interacting properties of the N-terminal domain of human apolipoprotein C-III (apo C-III) were investigated. By molecular modeling, we predicted that the 6-20 fragment of apo C-III is obliquely orientated at the lipid/water interface owing to an asymmetric distribution of the hydrophobic residues when helical. This is characteristic of 'tilted peptides' originally discovered in viral fusion proteins and later in various proteins including some involved in lipoprotein metabolism. Since most tilted peptides were shown to induce liposome fusion in vitro, the fusogenic capacity of the 6-20 fragment of apo C-III was tested on unilamellar liposomes and compared with the well characterized SIV fusion peptide. Mutants were designed by molecular modeling to assess the role of the hydrophobicity gradient in the fusion. FTIR spectroscopy confirmed the predominantly helical conformation of the peptides in TFE solution and also in lipid-peptide complexes. Lipid-mixing experiments showed that the apo C-III (6-20) peptide is able to increase the fluorescence of a lipophilic fluorescent probe. The vesicle fusion was confirmed by core-mixing and leakage assays. The hydrophobicity gradient plays a key role in the fusion process because the mutant with no hydrophobic asymmetry but the same mean hydrophobicity as the wild type does not induce significant lipid fusion. The apo C-III (6-20) fragment is, however, less fusogenic than the SIV peptide, in agreement with their respective mean hydrophobicity. Since lipid fusion should not be the physiological function of the N-terminal domain of apo CIII, we suggest that its peculiar distribution of hydrophobic residues is important for the lipid-binding properties of apo C-III and should be involved in apolipoprotein and lipid exchanges crucial for triglyceride metabolism. [less ▲]

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See detailStaalname van de hersenstam voor TSE-Onderzoek bij het schaap
Jacobs, C.; Gabriel, Annick ULg; Simoens, P.

in Vlaams Diergeneeskundig Tijdschrift (2002), 71

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See detailCréation d'un site Internet d'Evidence-Based Medicine.
Delvenne, Catherine ULg; Pasleau, Françoise ULg

in Médi-Sphère (2002), (67), 41-43

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See detailUnified stress update algorithms for the numerical simulation of large deformation elasto-plastic and elasto-viscoplastic processes
Ponthot, Jean-Philippe ULg

in International Journal of Plasticity (2002), 18(1), 91-126

This paper is concerned with unified stress update algorithms for elastoplastic and elasto-viscoplastic constitutive equations for metals submitted to large deformations. We present here a newly developed ... [more ▼]

This paper is concerned with unified stress update algorithms for elastoplastic and elasto-viscoplastic constitutive equations for metals submitted to large deformations. We present here a newly developed time integration algorithm which is, in the case of J2 flow theory material behavior, an extension to the viscoplastic range of the classical radial return algorithm for plasticity. The resulting unified implicit algorithm is both efficient and very inexpensive. Moreover, if there is no viscosity effect (rate-independent material) the presented algorithm degenerates exactly into the classical radial return algorithm for plasticity. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailComposite structures optimization using sequential convex programming
Bruyneel, Michaël ULg; Fleury, Claude ULg

in Advances in Engineering Software (2002), 33(7-10), 697-711

The design of composite structures is considered here. The approximation concepts approach is used to solve the optimization problem. The convex approximations of the MMA family are briefly described ... [more ▼]

The design of composite structures is considered here. The approximation concepts approach is used to solve the optimization problem. The convex approximations of the MMA family are briefly described. Several modifications of these approximations are presented. They are now based on gradient information at two successive iterations, avoiding the use of the expensive second-order derivatives. A two-point fitting scheme is also described, where the function value at the preceding design point is used to improve the approximation. Numerical examples compare these new purely non-monotonous schemes to the existing ones for the selection of optimal fibers orientations in laminates. It is shown how these two-point based approximations are well adapted to the problem and can improve the optimization task, leading to reasonable computational efforts. A procedure is also derived for considering simultaneously monotonous and non-monotonous structural behaviors. The resulting generalized approximation scheme is well suited for the optimization of composite structures when both plies thickness and fibers orientations are considered as design variables. It is concluded that the newly developed approximation schemes of the MMA family are reliable for composite structures optimization. All the studied approximations are convex and separable: the optimization problem can then be solved using a dual approach. (C) 2002 Civil-Comp Ltd and Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailJuvenile interphalangeal degenerative joint disease in the ardenner horse: radiographical evaluation's protocol (part II)
Lejeune, Jean-Philippe ULg; Schneider, Nicole ULg; Duvivier, Dominique Hannia et al

in Annales de Médecine Vétérinaire (2002), 146(3, JUN-JUL), 173-179

Juvenile interphalangeal degenerative joint disease is frequently observed in ardenner horses. The aim of this study is to established a radiographic examination's protocol of the distal limb and to apply ... [more ▼]

Juvenile interphalangeal degenerative joint disease is frequently observed in ardenner horses. The aim of this study is to established a radiographic examination's protocol of the distal limb and to apply it to ardenner horses. This investigation method takes assess of the standings, the conformation and of the potential lesions of the digits. An essential point of this protocol is the quantification of the asymmetrical articular compression of the interphalangeal joints. Thirty-two ardenner foals of 250 +/- 21 days were radiographied. The most frequently observed anomalies are first, the existence of abnormal standings such as distal interphalangeal flexion and external articular compression of the interphalangeal joints. Second, it shows in some cases, an abnormal conformation of the second phalanx. Finally, this protocol points out the existence of juvenile degenerative joint disease and of ossification of the fibrocartilages in some foals at this age. [less ▲]

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See detailLes Parents maltraités
Malchair, Alain ULg

in Neurone (2002), 7(3), 83-86

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See detailThree years experiment with the same tin oxide sensor arrays for the identification of malodorous sources in the environment
Romain, Anne-Claude ULg; Nicolas, Jacques ULg; Andre, Philippe ULg

in Sensors and actuators. B, Chemical (2002), (84), 271-277

The paper discusses the ability of recognition models to classify malodours in the environment 3 years after their calibration, with the same sources releasing odours. Two methodologies applicable either ... [more ▼]

The paper discusses the ability of recognition models to classify malodours in the environment 3 years after their calibration, with the same sources releasing odours. Two methodologies applicable either in the lab or in the field are used. The effect of the long term drift of the sensors is highlighted on the results of principal component analysis (PCA) and of discriminant function analysis (DFA). The paper examines also the applicability of some drift counteraction methods proposed in the literature. Handled with some care, a correction based on the drift direction in the principal components subspace should be applicable to the classification of real odorous sources in the field. [less ▲]

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See detailEvidence of an equilibrium between selenides and osmium(VIII) reagents and selenoxides and osmium(VI) reagents
Krief, A.; Destree, A.; Durisotti, V. et al

in Chemical Communications (2002), (6), 558-559

Driving the equilibrium between selenides and osmium( VIII) reagents with selenoxides and osmium( VI) by a subsequent reaction (rearrangement of allyl selenoxides to allyl alcohols or addition of osmium ... [more ▼]

Driving the equilibrium between selenides and osmium( VIII) reagents with selenoxides and osmium( VI) by a subsequent reaction (rearrangement of allyl selenoxides to allyl alcohols or addition of osmium( VIII) species on C C double bonds) to one side, allows the transformation of methyl geranyl selenides to linalool and of methyl citronellyl selenoxide to 6,7-dihydroxy citronellyl selenide. [less ▲]

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See detailUne étude inédite de Franz Cumont sur le vocabulaire des inscriptions funéraires chrétiennes de Gaule
Rochette, Bruno ULg

in Bulletin de l'Institut Historique Belge de Rome (2002), 72

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See detailKinetic study of the acid hydrolysis of various oligofructose samples
Blecker, Christophe ULg; Fougnies, C.; Van Herck, J. C. et al

in Journal of Agricultural and Food Chemistry (2002), 50(6), 1602-1607

The kinetic of acid hydrolysis of five commercially available oligofructose samples used as food ingredients has been investigated as a function of the dry matter concentration, reaction pH, and ... [more ▼]

The kinetic of acid hydrolysis of five commercially available oligofructose samples used as food ingredients has been investigated as a function of the dry matter concentration, reaction pH, and temperature. The initial fructose release rate is found to be roughly proportional to the inverse of the average polymerization degree in number. A pseudo first order kinetic is found with respect to the fructosyl chain end concentration and to the proton concentration. Arrhenius plot is found to reasonably fit the data in a relatively wide temperature range (7-130 degreesC). The results allow the estimation of the fructose release rate in many foodstuff processing conditions. [less ▲]

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See detailSingle-domain antibody fragments with high conformational stability.
Dumoulin, Mireille ULg; Conrath, Katja; Van Meirhaeghe, Annemie et al

in Protein Science : A Publication of the Protein Society (2002), 11(3), 500-15

A variety of techniques, including high-pressure unfolding monitored by Fourier transform infrared spectroscopy, fluorescence, circular dichroism, and surface plasmon resonance spectroscopy, have been ... [more ▼]

A variety of techniques, including high-pressure unfolding monitored by Fourier transform infrared spectroscopy, fluorescence, circular dichroism, and surface plasmon resonance spectroscopy, have been used to investigate the equilibrium folding properties of six single-domain antigen binders derived from camelid heavy-chain antibodies with specificities for lysozymes, beta-lactamases, and a dye (RR6). Various denaturing conditions (guanidinium chloride, urea, temperature, and pressure) provided complementary and independent methods for characterizing the stability and unfolding properties of the antibody fragments. With all binders, complete recovery of the biological activity after renaturation demonstrates that chemical-induced unfolding is fully reversible. Furthermore, denaturation experiments followed by optical spectroscopic methods and affinity measurements indicate that the antibody fragments are unfolded cooperatively in a single transition. Thus, unfolding/refolding equilibrium proceeds via a simple two-state mechanism (N <--> U), where only the native and the denatured states are significantly populated. Thermally-induced denaturation, however, is not completely reversible, and the partial loss of binding capacity might be due, at least in part, to incorrect refolding of the long loops (CDRs), which are responsible for antigen recognition. Most interestingly, all the fragments are rather resistant to heat-induced denaturation (apparent T(m) = 60-80 degrees C), and display high conformational stabilities (DeltaG(H(2)O) = 30-60 kJ mole(-1)). Such high thermodynamic stability has never been reported for any functional conventional antibody fragment, even when engineered antigen binders are considered. Hence, the reduced size, improved solubility, and higher stability of the camelid heavy-chain antibody fragments are of special interest for biotechnological and medical applications. [less ▲]

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See detailLymecycline and minocycline in inflammatory acne: a randomized, double-blind intent-to-treat study on clinical and in vivo antibacterial efficacy.
Pierard, Claudine ULg; Goffin, Véronique ULg; Arrese Estrada, Jorge ULg et al

in Skin Pharmacology & Applied Skin Physiology (2002), 15(2), 112-9

BACKGROUND: Some antibiotics represent a mainstay in acne treatment. However, studies comparing their efficacies are rare. AIM: To evaluate the clinical and in vivo antibacterial effect of lymecycline and ... [more ▼]

BACKGROUND: Some antibiotics represent a mainstay in acne treatment. However, studies comparing their efficacies are rare. AIM: To evaluate the clinical and in vivo antibacterial effect of lymecycline and minocycline at different dosages. METHOD: Eighty-six patients with moderate to severe acne were enrolled in a randomized, double-blind, intent-to-treat study comparing in three parallel groups the effect of (1) lymecycline 300 mg daily for 12 weeks, (2) minocycline 50 mg daily for 12 weeks and (3) minocycline 100 mg daily for 4 weeks followed by 50 mg daily for 8 weeks. Evaluations were made at the screening visit and at five on-treatment visits. They consisted of clinical counts of acne lesions and evaluations of bacterial viability using dual flow cytometry performed on microorganisms collected from sebaceous infundibula by cyanoacrylate strippings. RESULTS: Patients receiving minocycline 100/50 mg had the best clinical outcome, particularly in the reduction of the number of papules. By the end of the trial, the microbial response to minocycline 100/ 50 mg was also superior to either of the other two treatments. There were less live and more dead bacteria. CONCLUSION: In this trial, minocycline 100/50 mg was superior for the treatment of inflammatory acne when compared to lymecycline 300 mg and minocycline 50 mg. [less ▲]

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See detailDe la pluridisciplinarité à l'interdisciplinarité. Réflexions critiques sur la formation des enseignants en sciences sociales
Guillaume, Jean-François ULg; Barras, Dominique; Xhonneux, Michel ULg

in Puzzle (2002), 11

Réflexion sur le dispositif de formation des enseignants en sciences sociales, à l'Université de Liège, et sur les conditions requises pour une approche "interdisciplinaire".

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See detailRefroidissement de +32°C à +5°C du sperme dilué de taureau de race Borgou au Bénin
Adamou-N'Diaye, M.; Gbangboche, A. B.; Adjovi, A. et al

in Annales des Sciences Agronomiques du Bénin (2002), 3(1), 81-93

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See detailSite-directed mutagenesis studies of the hypoxia-inducible factor-1alpha DNA-binding domain.
Michel, Gaetan; Minet, Emmanuel; Mottet, Denis ULg et al

in Biochimica et Biophysica Acta (2002), 1578(1-3), 73-83

Hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor, is activated when cells are exposed to hypoxia. It is composed of two subunits, HIF-1alpha and ARNT. When activated, it binds to ... [more ▼]

Hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor, is activated when cells are exposed to hypoxia. It is composed of two subunits, HIF-1alpha and ARNT. When activated, it binds to the hypoxia-responsive element (HRE) and up-regulates the expression of several genes (vascular endothelial growth factor (VEGF), erythropoietin (EPO), enolase, em leader ). By molecular modeling, a 3-D model for the complex between the DNA-binding domain of HIF-1 (bHLH domain) and its consensus DNA sequence has been developed. Specific interactions between three amino acids (Ser22, Ala25, Arg30) of the HIF-1alpha subunit and DNA bases were identified. In order to further investigate the role of these amino acids, we generated four mutants of the HIF-1alpha subunit using site-directed mutagenesis. The activity of each mutant was tested using a reporter gene containing either 6 HRE sequences or the authentic human VEGF promoter. The results show that three mutants, Ala25Ser, Ala26Glu and Arg30Ala, were no longer active in the reporter gene assay. On the other hand, the Ser22Ala mutant increased the reporter gene expression, in normoxia as well as in hypoxia. These results correlate with the ones obtained when the DNA-binding capability of the mutants was assayed by electrophoretic mobility shift assays (EMSA): Arg30Ala and Ala26Glu mutants bind very weakly to HRE while the Ser22Ala mutant has the same binding capacity as the wild-type HIF-1alpha. These results bring new insights on the specificity of the protein/DNA interactions for HIF-1 towards HRE. [less ▲]

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See detailA multistage field test of wheelchair users for evaluation of fitness and prediction of peak oxygen consumption
Vanderthommen, Marc ULg; Francaux, M.; Colinet, Cédrik et al

in Journal of Rehabilitation Research & Development (2002), 39(6, Nov-Dec), 685-692

An incremental multistage field test (MFT) for wheelchair users was developed to evaluate physical fitness and predict peak oxygen consumption (VO2). Using auditory feedback, the participants (n = 37 ... [more ▼]

An incremental multistage field test (MFT) for wheelchair users was developed to evaluate physical fitness and predict peak oxygen consumption (VO2). Using auditory feedback, the participants (n = 37) were directed to wheel around an octagonal course, increasing their velocity every minute until exhaustion. Wheelchair velocity and metabolic parameters were recorded with the use of a speedometer and a portable spirometer system. The average number of exercise levels performed (MFT score) was 9.17 +/- 5.81, resulting in a peak heart rate (HR) of 99.0 +/- 13.9% of the theoretical maximum. A test-retest analysis (n = 10) showed that the MFT was reliable regarding MFT score, peak VO2, and peak HR reached. Stepwise multiple regression based on individual, wheelchair, propulsion technique, and physiological parameters revealed that the MFT score was the best and only predictor of peak VO2 (mL/min/kg) (= 18.03 + 0.78 MFT score, r(2) = 0.59). The MFT assesses wheelchair mobility and estimates peak VO2 encountered during the test. [less ▲]

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See detailAromatic Side-Chain Interactions In Proteins. II. Near- And Far-Sequence Phe-X Pairs
Thomas, Annick ULg; Meurisse, R.; Brasseur, Robert ULg

in Proteins-Structure Function and Genetics (2002), 48(4), 635-44

We have collected all aromatic pairs (3152) involving an N-phenyl partner in a dataset of 593 proteins of the PDB: 728 of these pairs involve a partner residue less than 6 apart in the sequence. These ... [more ▼]

We have collected all aromatic pairs (3152) involving an N-phenyl partner in a dataset of 593 proteins of the PDB: 728 of these pairs involve a partner residue less than 6 apart in the sequence. These near-sequence Phe-X pairs correspond to specific conformations that stabilize secondary structures, mainly alpha-helices when the residues are 1, 3, and 4 apart, and beta-strands when they are 2 apart in the sequence. These conformations are not spatially random and have been examined in detail. The remaining phenylalanine pairs (2424) are between partners more than 5 apart in the sequence. Of these far-sequence pairs, 34% of occurrences are in sheets. Next in frequencies are pairs that bridge a beta-strand to a helix (24%), followed by pairs that bridge a beta-strand to a random coiled structure (15%). Helix to helix pairs only constitute 12% of these far-sequence pairs. Analysis of the pairing frequency supports the hypothesis that aromatic interactions are late events of protein folding. [less ▲]

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See detailActualites therapeutiques 2002 (1).
Scheen, André ULg

in Revue Médicale de Liège (2002), 57(11), 719-31

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The main characteristics of each molecule and ... [more ▼]

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The main characteristics of each molecule and its modalities of appropriate use in clinical practice will be emphasized. For all these molecules, both the efficacy and tolerance have been proven in randomised clinical trials according to the rules of Evidence-Based Medicine. [less ▲]

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See detailThe 2.4-A crystal structure of the penicillin-resistant penicillin-binding protein PBP5fm from Enterococcus faecium in complex with benzylpenicillin.
Sauvage, Eric ULg; Kerff, Frédéric ULg; Fonze, E. et al

in Cellular and Molecular Life Sciences : CMLS (2002), 59(7), 1223-32

Penicillin-binding proteins (PBPs) are membrane proteins involved in the final stages of peptidoglycan synthesis and represent the targets of beta-lactam antibiotics. Enterococci are naturally resistant ... [more ▼]

Penicillin-binding proteins (PBPs) are membrane proteins involved in the final stages of peptidoglycan synthesis and represent the targets of beta-lactam antibiotics. Enterococci are naturally resistant to these antibiotics because they produce a PBP, named PBP5fm in Enterococcus faecium, with low-level affinity for beta-lactams. We report here the crystal structure of the acyl-enzyme complex of PBP5fm with benzylpenicillin at a resolution of 2.4 A. A characteristic of the active site, which distinguishes PBP5fm from other PBPs of known structure, is the topology of the loop 451-465 defining the left edge of the cavity. The residue Arg464, involved in a salt bridge with the residue Asp481, confers a greater rigidity to the PBP5fm active site. In addition, the presence of the Val465 residue, which points into the active site, reducing its accessibility, could account for the low affinity of PBP5fm for beta-lactam. This loop is common to PBPs of low affinity, such as PBP2a from Staphylococcus aureus and PBP3 from Bacillus subtilis. Moreover, the insertion of a serine after residue 466 in the most resistant strains underlines even more the determining role of this loop in the recognition of the substrates. [less ▲]

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See detailAdvantages and disadvantages of a control programme against infectious bovine rhinotracheitis in Belgium
Limbourg, B.; Kerkhofs, P.; Massard, C. et al

in Annales de Médecine Vétérinaire (2002), 146(2, APR-MAY), 57-69

A working party of the "Association d'Epidemiologie et de Sante Animale" assessed the strategy for a control of infectious bovine rhinotracheitis (IBR) in Belgium and studied its feasability and the ... [more ▼]

A working party of the "Association d'Epidemiologie et de Sante Animale" assessed the strategy for a control of infectious bovine rhinotracheitis (IBR) in Belgium and studied its feasability and the different programmes to be applied. Epidemiology of IBR in Belgium, vaccination, diagnostic methods, economic aspects of IBR control, Belgian laws regarding IBR and the motivation of people in the field were described. The advantages and disadvantages of three programmes were analysed: to maintain the current situation, to protect cattle against clinical disease and to control and eradicate IBR. [less ▲]

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See detailL'étude MICROHOPE
Krzesinski, Jean-Marie ULg

in Journal de Cardiologie = Tijdschrift voor Cardiologie (2002), 14(5), 170-172

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See detailDe la philosophie à la sociologie en compagnie de Pierre Bourdieu
Frère, Bruno ULg

in Revue de l'Institut de Sociologie (2002), 1-4

La compréhension adéquate des influences de Pierre Bourdieu peuvent nous permettre de diversifier sa pensée comme il diversifiait lui-même celle des autres. On sait qu’il utilise Wittgenstein dès l ... [more ▼]

La compréhension adéquate des influences de Pierre Bourdieu peuvent nous permettre de diversifier sa pensée comme il diversifiait lui-même celle des autres. On sait qu’il utilise Wittgenstein dès l'élaboration de son vocabulaire critique défiant les « prénotions » et les préjugés condamnés comme étant le fruit d'une sociologie spontanée et profane qui mine le jugement objectif. Mais il le quitte au moment où celui-ci manifeste le désir de revenir « au point de vue du sens commun ». Il semble bien que nous ayons alors affaire à « une quête fervente de l'ordinaire et du familier » à laquelle Bourdieu ne se référera plus.. En tenir compte peut nous permettre d’ouvrir le dialogue avec la sociologie pragmatique de Luc Boltanski et Laurent Thévenot. A la suite de Bourdieu, c’est alors en partie avec eux qu’on peut prolonger véritablement l’exigence wittgensteinienne : étudier les « exemples primitifs des langages communs ». Ces sociologies, on le sait, accueillent a priori le discours des acteurs comme le fruit d’un travail interprétatif cohérent. Qu’ils soient « justes » ou non par rapport à l’état du monde n’est pas l’essentiel. L’enjeu n’est plus d’évaluer leur valeur de vérité par rapport à la réalité - ce qu’autorise la pensée de Bourdieu - mais d’approcher « des sujets parlants ordinaires [… ]co-énonciateurs du discours intervenant en permanence dans la construction du sens » . Tous sont capables de construire et de déconstruire leurs pratiques et ce jeu de construction-déconstruction revêt un intérêt en soi. Il s’agit moins de nier le fait qu’un langage quelconque puisse être pétri d’illusions que de chercher à en saisir la portée significative. En définitive, les objectifs de ces paradigmes sont à la fois différents et tout à fait complémentaires. Il faut à présent chercher à saisir correctement les objets sociologiques ambigus qu’une sociologie hybride permettrait d’éclairer. C'est ce que nous nous suggérons de le présent article. [less ▲]

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See detailThe concept of age in marine modelling II. Concentration distribution function in the English Channel and the North Sea
Delhez, Eric ULg; Deleersnijder, Eric

in Journal of Marine Systems (2002), 31(4), 279-297

The age of seawater and the age of real or idealized tracers are often used as diagnostic tools to better understand complex hydrodynamic flows. In most studies, the focus is on some averages of the ages ... [more ▼]

The age of seawater and the age of real or idealized tracers are often used as diagnostic tools to better understand complex hydrodynamic flows. In most studies, the focus is on some averages of the ages of the different particles making up a water parcel. The theory developed in Delhez et al. [Ocean Modell. I (1999) 17] and Deleersnijder et al. [J. Mar. Syst. 28 (2001) 229] provides, however, a more detailed description of the distribution of the ages of these particles through the so-called concentration distribution function. In this paper, the numerical aspects of the resolution of the evolution equation for the concentration distribution function in a five-dimensional space (time x 3D space x age dimension) are developed. Evolution equations for the moments of the concentration distribution function up to any order are also derived. A real case application of this theory to the simulation of the advection-dispersion of tracers (technetium-99) discharged at the nuclear fuel reprocessing plant of Cap de La Hague is described. The comparison of the results with those from previous studies demonstrates the advantages of the new method for the computation of the mean age. In particular, the method provides a detailed description of the temporal variations of the mean age only. The analysis of the full concentration distribution function and of its basic statistics shows that the standard deviation of the age of the different particles is far from negligible and should never be overlooked when analyzing age fields. A simplified analytical example suggests that the standard deviation of the age distribution is a measure of the integrated diffusion undergone by the tracer along its path from the source to the observation point. (C) 2002 Elsevier Science B.V All rights reserved. [less ▲]

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See detailPéritexte auctorial ou éditorial ? Réflexions théoriques et suggestions pratiques
Vanhaegendoren, Koen ULg

in Annales de l’Université de Craiova, Série de littérature roumaine et comparée (2002), 24(1-2), 184-192

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See detailComparison of Ricotta cheese made by high pressure treatment with that produced by heat treatment of sweet whey
Besbes, S.; Blecker, Christophe ULg; Attia, H. et al

in Sciences des Aliments (2002), 22(5), 601-615

In this study, reconstituted sweet whey was acidified and then treated by high hydrostatic pressure in order to obtain Ricotta-like product. At pH 5.2, whey protein aggregation was effective beyond 200 ... [more ▼]

In this study, reconstituted sweet whey was acidified and then treated by high hydrostatic pressure in order to obtain Ricotta-like product. At pH 5.2, whey protein aggregation was effective beyond 200 MPa for an application time of 30 min. Moist yield was appreciably improved when pressurisation time increased at 400 MPa. It seemed that granulometric properties of Ricotta-like cheese were not affected by increasing pressurisation time. Ricotta-like products obtained after pressurisation at 400 MPa for 30, 45 and 60 min, have shown a lower yield and hardness but a higher cohesiveness than traditional Ricotta cheese. They also had more homogenous granulometric profiles. [less ▲]

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See detailMétastase synoviale d'un cancer colique se présentant comme une arthrite du genou. Caractérisation par tomographie à émission de positons au 18FDG
Beckers, C.; Foidart, J.; Thiry, Albert ULg et al

in Revue Médicale de Liège (2002), 57(5), 467-474

A case of carcinomatous monoarthritis involving the left knee due to colonic adenocarcinoma is described. Large recurrent synovial effusion, that will be later hematic, lytic lesion of the bones and ... [more ▼]

A case of carcinomatous monoarthritis involving the left knee due to colonic adenocarcinoma is described. Large recurrent synovial effusion, that will be later hematic, lytic lesion of the bones and chondrolysis were noted. Knee positron emitting tomography scan using FDG (FDG-PET) revealed a diffuse increased uptake in soft tissues assumed to be synovium, the hypertrophy of which was identified by ultrasonography. Whole body PET scan showed extensive lymph node, visceral and bone metastases, suggesting that the increase in the synovium could also be of metastatic origin. The final diagnosis of synovial carcinomatosis secondary to the known colonic adenocarcinoma was confirmed by histological analysis of biopsies obtained by arthroscopy. A review of the literature is realised. To our knowledge, this is the first synovial metastasis studied by FDG-PET. [less ▲]

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See detailApplication of the laser beam deflection to the study of temperature filed and heat transfer in natural convection,
Cerisier, P.; Sylvain, J. D.; Dauby, Pierre ULg

in Experiments in Fluids (2002), 33

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See detailLe contrat de travail et la signature électronique
Kefer, Fabienne ULg; Barth, H.

in Orientations : la Revue du Droit Social et de la Gestion du Personnel (2002)

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See detailCapacity utilisation and profitability: A decomposition of short-run profit efficiency
Coelli, Tim; Grifell-Tatjé, Emili; Perelman, Sergio ULg

in International Journal of Production Economics (2002), 79

The principal aim of this paper is to measure the amount by which the profit of a multi-input, multi-output firm deviates from maximum short-run profit, and then to decompose this profit gap into ... [more ▼]

The principal aim of this paper is to measure the amount by which the profit of a multi-input, multi-output firm deviates from maximum short-run profit, and then to decompose this profit gap into components that are of practical use to managers. In particular, our interest is in the measurement of the contribution of unused capacity, along with measures of technical inefficiency, and allocative inefficiency, in this profit gap. We survey existing definitions of capacity and, after discussing their shortcomings, we propose a new ray economic capacity measure that involves shortrun profit maximisation, with the output mix held constant. We go on to describe how the gap between observed profit and maximum profit can be calculated and decomposed using linear programming methods. The paper concludes with an empirical illustration, involving data on 28 international airline companies. The empirical results indicate that these airline companies achieve profit levels which are on average US$815m below potential levels, and that 70% of the gap may be attributed to unused capacity. [less ▲]

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See detailEvaluation of the Hycel Diagnostics Diana-5 Hematology Analyzer
Boemer, François ULg; Cielniaszek, Nadine; Paulus, Jean-Michel ULg et al

in Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology (2002), 8

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision ... [more ▼]

The Hycel Diagnostics Diana-5, a compact 5-part differential hematology analyzer, was evaluated in the hematology laboratory of Liège University Hospital, Belgium. Within-and between-batch precision, linearity, and absence of carryover were all excellent. All parameters were stable for 48 hours at 4 ° C and for 8 hours at room temperature. Comparability of complete blood counts to Bayer Technicon Advia 120 measurements was highly satisfactory, with the exception of the mean cell volume, which was slightly underestimated by the Diana-5 compared to the Advia 120. Comparisons of differential leukocyte counts to 400-cell microscopic differentials were excellent for neutrophils, lymphocytes, and eosinophils and were acceptable for monocytes. The efficiency of the flagging system was high for the detection of blasts and less satisfactory for the detection of immature granulocytes and atypical lymphocytes. The instrument was reliable and easy to use. [less ▲]

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See detailDonnées actuelles sur la toxicité de l'oxygène
Deby, Ginette ULg; Deby, C.; Lamy, Maurice ULg

in Réanimation (2002), 11(1), 28-39

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See detailLe medicament du mois. La rosiglitazone (Avandia).
Scheen, André ULg

in Revue Médicale de Liège (2002), 57(4), 236-9

Rosiglitazone (Avandia, Glaxo-SmithKline) belongs to a new family of oral hypoglycaemic agents, thiazolidinediones or glitazones. These molecules act as selective agonists of nuclear receptors (PPAR gamma ... [more ▼]

Rosiglitazone (Avandia, Glaxo-SmithKline) belongs to a new family of oral hypoglycaemic agents, thiazolidinediones or glitazones. These molecules act as selective agonists of nuclear receptors (PPAR gamma) and improve insulin sensitivity. In Belgium as in all European countries, rosiglitazone is indicated for the treatment of type 2 diabetes, only in combination with another antidiabetic oral agent, in patients insufficiently controlled with metformin or a sulphonylurea at a maximal tolerated dose. In these patients, rosiglitazone, at a daily dose of 4 mg (sometimes 8 mg/day with metformin), reduces fasting glycaemia by 2-3 mmol/l and glycated haemoglobin level by about 1%. It exerts also favourable effects on some risk factors related to insulin resistance syndrome, which may contribute to improve cardiovascular prognosis of patients with type 2 diabetes. Hepatic safety of rosiglitazone seems to be good, although it is still recommended to check liver enzymes regularly. As all glitazones, rosiglitazone moderately promotes weight gain. It can also induce some fluid retention which may reveal or aggravate heart failure in at risk patients. [less ▲]

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See detailLes conditions d'un bien-être pour les animaux de rente.
Vandenheede, Marc ULg

in Les Elevages Belges (2002), 4

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See detailCuttings of the non-rooting rac tobacco mutant overaccumulate phenolic compounds
Faivre-Rampant, Odile; Charpentier, Jean Paul; Kevers, Claire ULg et al

in Functional Plant Biology (2002), 29(1), 63-71

The auxin and phenolic contents, as well as phenylalanine ammonia-lyase (PAL) activity, were determined in in vitro cultured shoots of the recalcitrant-to-root rac mutant of tobacco, and compared with ... [more ▼]

The auxin and phenolic contents, as well as phenylalanine ammonia-lyase (PAL) activity, were determined in in vitro cultured shoots of the recalcitrant-to-root rac mutant of tobacco, and compared with wild-type shoots. The mutant and wild-type shoots showed similar auxin changes during the culture cycle, but with higher contents for the mutant. A transient peak of auxin (corresponding to the achievement of the rooting inductive phase) occurred at day 14 in both types of shoots, but earlier in the basal parts of the wild-type stems. The rac shoots contained more phenolics, corresponding with an increased PAL activity. The most abundant phenolic compound found in the two types of tobacco was chlorogenic acid, which was more abundant in the rac shoots. Rutin was also detected at a higher concentration in the mutant shoots. Basal parts of wild-type shoots treated with 10(-3) chlorogenic acid reacted by accumulating auxins and, unlike untreated controls, did not form adventitious roots. The relationships between those biochemical analyses in relation to the growth limitation of the rac mutant, and the inhibition of its root development, are discussed. [less ▲]

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See detailInvasive tricholemmal carcinoma of the nose.
Van Zele, Dirk; Arrese Estrada, Jorge ULg; Heymans, Olivier et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (2002), 204(4), 315-7

Tricholemmal carcinoma is a rare entity. It must be distinguished from the benign proliferating variant of the tricholemmal cyst. We report a case of tricholemmal carcinoma massively invading the nose ... [more ▼]

Tricholemmal carcinoma is a rare entity. It must be distinguished from the benign proliferating variant of the tricholemmal cyst. We report a case of tricholemmal carcinoma massively invading the nose. The neoplasm was excised surgically with a satisfactory outcome. [less ▲]

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See detailIncreased Cell Proliferation, But Not Reduced Cell Death, Induces Lymphocytosis In Bovine Leukemia Virus-Infected Sheep
Debacq, C.; Asquith, B.; Kerkhofs, P. et al

in Proceedings of the National Academy of Sciences of the United States of America (2002), 99(15),

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See detailLa dignité humaine comme concept juridique
Fierens, Jacques ULg

in Journal des Tribunaux (2002)

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See detailLes facteurs neurotrophiques : un mythe thérapeutique ?
Rogister, Bernard ULg

in Ahead in Neurology (2002), 5(1), 3-20

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See detailComparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma
Perenyi, Z. S.; Szenci, Otto; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2002), 37(2), 100-104

Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression pattern. When PAGs are released in the maternal blood they can be used for pregnancy diagnosis, pregnancy follow-up and for the monitoring of the trophoblastic function. Three different radioimmunoassay systems (RIA 1, RIA 2 and RIA 3) using antisera produced against PAG I67 (RIA 1), PAG55+62 (RIA 2) and PAG55+59 (RIA 3) were used in this investigation in order to measure the PAG concentration in plasma samples withdrawn from pregnant cows and heifers during different periods following artificial insemination (AI). These systems were able to detect PAG molecules in the maternal blood as early as 21 days after AI in different concentrations (RIA 1: 0.43 +/- 0.24 ng/ml, mean +/- SD; RIA 2: 0.48 +/- 0.24 ng/ml; RIA 3: 0.64 +/- 0.37 ng/ml). On days 32 and 42 RIA 2 (4.30 +/- 1.32 ng/ml and 5.56 +/- 1.95 ng/ml) and RIA 3 (4.17 +/- 1.15 ng/ml and 5.60 +/- 1.89 ng/ml) presented significantly (p < 0.0001) higher PAG concentrations than those of RIA 1 (2.43 +/- 0.81 ng/ml and 4.01 +/- 1.48 ng/ml), respectively. After day 21, significant correlations (p < 0.0001; r >/= 0.929) were determined between the three systems. Additionally the three individual PAG profiles presented in this study showed that PAG molecules secreted in the maternal blood between 21 and 50 days after AI were better recognized by the RIA 2 and RIA 3 systems. This study clearly indicated that the ability of a RIA test to recognize PAG molecules in the maternal blood can be improved by carefully selecting the antiserum. [less ▲]

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See detailPrediction of carcass lean content by real-time ultrasound in Piétrain and negative stress Piétrain
Youssao, A. K. L.; Verleyen, Vincent ULg; Leroy, Pascal ULg

in Animal Science (2002), 75

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See detailDes pratiques aux performances : la littératie chez les jeunes de 15 ans
Lafontaine, Dominique ULg

in Lettre de la DFLM (La) (2002), 30

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See detailVolumineuse masse pelvienne chez un homme jeune
Andrianne, Robert ULg; Detroz, Bernard ULg; Delbecque, Katty ULg et al

in Revue Médicale de Liège (2002), 57(3), 148-154

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See detailL'allergie aux aromes et parfums: anathème sur un attrait épicurien?
Goffin, Véronique ULg; Nikkels, Arjen ULg; Cornil, F. et al

in Revue Médicale de Liège (2002), 57(9), 605-9

Aromas and fragrances are present in many cosmetics, some topical drugs, food and various hygiene, household and industrial products. They can be responsible for contact dermatitis. Multiple ... [more ▼]

Aromas and fragrances are present in many cosmetics, some topical drugs, food and various hygiene, household and industrial products. They can be responsible for contact dermatitis. Multiple sensitizations can even involve in various combinations some fragrance compounds, a given degradation product or a contaminant. The diagnosis relies on clinical examination and oriented anamnesis. A histological examination is sometimes necessary. Specific path testing brings insight on the culprit chemical compounds. [less ▲]

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See detailCharacterisation of aphid myrosinase and degradation studies of glucosinolates
Francis, Frédéric ULg; Lognay, Georges ULg; Wathelet, Jean-Paul ULg et al

in Archives of Insect Biochemistry and Physiology (2002), 50(4), 173-182

Myrosinase from Brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a DEAE column. The chromatography yielded a single peak and a 115.6-fold purification ... [more ▼]

Myrosinase from Brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a DEAE column. The chromatography yielded a single peak and a 115.6-fold purification. Further FPLC gel filtration gave a single peak at 120 kDa. Denaturing SDS/PAGE of the protein revealed a single band at 60 kDa, indicating that the native B. brassicae myrosinase is a dimer. Kinetic parameters towards 8 glucosinolates were calculated. Strong differences of V-max and Km were observed depending on the substrate. Degradation products of each glucosinolate were identified and quantified by GC-MS and GLC-FID, respectively. Using both crude aphid homogenates and purified myrosinase, two unique hydroxyglucosinolates, 3-butenyl- and benzyl-isothiocyanates were identified from progoitrin ((2S)-2-hydroxybut-3-enyl-glucosinolate) and sinalbin (4-hydroxybenzyl-glucosinolate) degradation respectively. Addition of ascorbic acid to the reaction mixtures containing sinalbin and progoitrin caused the production of hydroxyloted degradation products usually associated with plant myrosinase metabolisation. The occurrence of the myrosinase system in B. brassicae is discussed in terms of similar allelochemical adaptation between the herbivore and its host plant. (C) 2002 Wiley-Liss, Inc. [less ▲]

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See detailL'école médicale d'Alexandrie et son influence sur la médecine de l'Egypte gréco-romaine
Marganne, Marie-Hélène ULg

in Medicina nei Secoli : Arte e Scienza (2002), 14(2), 359-82

The paper provides new information on the most famous centre of rational medicine in the Graeco-Roman world and its influence in Egypt. It uses at its point of departure a remarkable but insufficiently ... [more ▼]

The paper provides new information on the most famous centre of rational medicine in the Graeco-Roman world and its influence in Egypt. It uses at its point of departure a remarkable but insufficiently known documentation: Greek literary papyri (from IV/III B.C. to A.D. VI/VII), which often are unique witnesses to lost medical works, bearing testimony to original theories, practices and vocabulary. [less ▲]

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See detailDifferential taurine responsiveness to ethanol in high- and low-alcohol sensitive rats : a brain microdialysis study
Quertemont, Etienne ULg; Linotte, Sylvie; De Witte, Philippe

in European Journal of Pharmacology (2002), 444(3), 143-150

Several microdialysis studies have investigated the effects of acute ethanol on extracellular amino acids in various rat brain regions, However, these studies led to conflicting results, suggesting that ... [more ▼]

Several microdialysis studies have investigated the effects of acute ethanol on extracellular amino acids in various rat brain regions, However, these studies led to conflicting results, suggesting that individual differences between rat strains and lines may play an important role. In the present study, high-alcohol sensitive (HAS) and low-alcohol sensitive (LAS) rats were used to investigate the possible relationship between ethanol sensitivity and the concentrations of extracellular amino acids in the nucleus accumbens. Several groups of HAS and LAS rats were injected with either saline or ethanol (1.0, 2.0 or 3.0 g/kg, i.p.) and the concentrations of amino acids in the nucleus accumbens microdialysates were assayed by electrochemical detection. Acute ethanol induced a dose-dependent increase in extracellular taurine concentrations. However, this increase was significantly reduced at 2,0 and 3.0 g,,kg ethanol in HAS rats relative to LAS rats. Since the biological functions of taurine suggest its implication in the reduction of ethanol adverse effects, a higher increase in taurine concentrations may contribute to the lower ethanol sensitivity of LAS rats. Although 2.0 and 3.0 g/kg ethanol did not affect extracellular glutamate concentrations, a significant increase in glutamate was observed after 1.0 g/kg ethanol to HAS rats but not to LAS rats. Such an effect remains unexplained but suggests that discrepancies between the results of previous microdialysate studies may be related to differences in the ethanol sensitivities of various rat strains. (C) 2002 Elsevier Science B.V All rights reserved. [less ▲]

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See detailWeapons of Mass Destruction Export Control Regime : From Export Control List to Catch-all Clause
Michel, Quentin ULg

in Studia Diplomatica (2002), Vol. LV : 2002(n°5/6), 39-50

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See detailEffect of deep digital flexor tendon orientation on magnetic resonance imaging signal intensity in isolated equine limbs - The magic angle effect
Busoni, Valeria ULg; Snaps, Frédéric ULg

in Veterinary Radiology & Ultrasound (2002), 43(5, Sep-Oct), 428-430

Ten normal equine isolated limbs were imaged using a knee coil in a 1.5 Tesla magnetic field, with short echo time sequences (TE < 15 ms). Magnetic resonance imaging was performed on each isolated limb in ... [more ▼]

Ten normal equine isolated limbs were imaged using a knee coil in a 1.5 Tesla magnetic field, with short echo time sequences (TE < 15 ms). Magnetic resonance imaging was performed on each isolated limb in different positions, with and without extension of the metacarpophalangeal joint. Deep digital flexor tendon orientation ranged from 20 to 60 degrees in relation to the static magnetic field. Increased intratendinous signal intensity was observed when the angle between the deep digital flexor tendon and the constant magnetic field approached 55 degrees ("magic angle"). The increased signal intensity was independent from extension of the metacarpophalangeal joint. Recognition of the magic angle phenomenon is essential for proper evaluation of magnetic resonance imaging studies of the equine foot. [less ▲]

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See detailPhylogeny of the genus Apodemus with a special emphasis on the subgenus Sylvaemus using the nuclear IRBP gene and two mitochondrial markers: cytochrome b and 12S rRNA.
Michaux, Johan ULg; Chevret, P.; Filippucci, M*-G et al

in Molecular Phylogenetics & Evolution (2002), 23(2), 123-36

Phylogenetic relationships among 17 extant species of Murinae, with special reference to the genus Apodemus, were investigated using sequence data from the nuclear protein-coding gene IRBP (15 species ... [more ▼]

Phylogenetic relationships among 17 extant species of Murinae, with special reference to the genus Apodemus, were investigated using sequence data from the nuclear protein-coding gene IRBP (15 species) and the two mitochondrial genes cytochrome b and 12S rRNA (17 species). The analysis of the three genes does not resolve the relationships between Mus, Apodemus, and Rattus but separates Micromys from these three genera. The analysis of the two mitochondrial regions supported an association between Apodemus and Tokudaia and indicated that these two genera are more closely related to Mus than to Rattus or Micromys. Within Apodemus, the mitochondrial data sets indicated that 8 of the 9 species analyzed can be sorted into two main groups: an Apodemus group, with A. agrarius, semotus, and peninsulae, and a Sylvaemus group, with uralensis, flavicollis, alpicola, sylvaticus, and hermonensis. The position of Apodemus mystacinus is ambiguous and might be either included in Sylvaemus or considered a distinct subgenus, Karstomys, more closely related to Sylvaemus than to Apodemus. Estimation of the divergence time for these taxa suggests a separation between 7 and 8 My ago for the three groups (mystacinus and the two subgenera Apodemus and Sylvaemus). Within each subgenus, divergence times are between 5.4 and 6 My for Apodemus and between 2.2 and 3.5 My for Sylvaemus and mystacinus. [less ▲]

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See detailÉtude dendrométrique d’Acacia auriculiformis A. Cunn. ex Benth. en mélange sur vertisol au Bénin
Fonton, N. H.; Glele Kakai, R.; Rondeux, Jacques ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 29-37

Acacia auriculiformis is a fast growing species planted on vertisols in Benin. An inventory of 11 mixed stands of Acacia, which are 7 to 9 years old, has been realized to estimate growth parameters of the ... [more ▼]

Acacia auriculiformis is a fast growing species planted on vertisols in Benin. An inventory of 11 mixed stands of Acacia, which are 7 to 9 years old, has been realized to estimate growth parameters of the stands. The present study shows that, for the dendrometrical parameters, there is no difference between species associations at a 0.05 significance level. Nevertheless, the largest values of quadratic mean diameter of the stems foot are observed in the species associations including Leucaena leucocephala. Moreover, the branch volume decreases with the increasing of the number of stems per tree. The most appropriate volume function for Acacia auriculiformis is the one that takes into account the diameter of the stems foot and the total height. Otherwise, the species associations do not influence the profile of Acacia trees, which is constant within the associations. The predicted conversion and cutting coefficients show that the stacked wood volume represents about 144 % of the real stand volume. [less ▲]

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See detailLes herpèsviroses des petits ruminants
Thiry, Etienne ULg; Keuser, Véronique; Tempesta, Maria

in Point Vétérinaire (2002), 33(74-76),

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See detailLa loi du 17 avril 2002 instaurant la peine de travail comme peine autonome en matière correctionnelle et de police
Jacobs, Ann ULg; Dantinne, Michaël ULg

in Revue de Droit Pénal et de Criminologie (2002)

Présentation et analyse juridique et criminologique de la loi du 17 avril 2002 introduisant la peine de travail en droit belge.

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See detailFcpac: Fast Calculation Of Partial Atomic Charges Using Strictly Localized Molecular Orbitals
Swinnen, M.; Thomas, Annick ULg; Brasseur, Robert ULg

in Computational Materials Science (2002), 25(4), 590-595

Most force fields of molecular mechanics use constant partial atomic charges whereas it is now admitted that those charges strongly vary with the environment. Charge variations are particularly important ... [more ▼]

Most force fields of molecular mechanics use constant partial atomic charges whereas it is now admitted that those charges strongly vary with the environment. Charge variations are particularly important in hydrogen bonds where polar entities come close together. Moreover, organizing H-bonds in networks, like in secondary structures of proteins, go with cooperative effects which lead to an overall electrostatic stabilization of the system. This is why variable atomic charges are required to correctly mimic these effects. Different methods of charge calculation were developed, most use polarizable dipole or electronegativity equalization. In this work, we propose a semi-empirical method (fast calculation of partial atomic charges (FCPAC)) which derives partial atomic charges from strictly localized molecular orbitals. The approximations enable to treat molecular systems with hundreds of atoms within reasonable delays. Results reported here show that charges calculated with FCPAC are similar to charges derived from Mulliken’s population analysis of a restricted Hartree–Fock wave function using the split valence basis set 6-31G and including polarization orbitals p and d respectively for hydrogen and heavy atoms, RHF/6-31G(d,p). Comparison with ab initio calculation was also performed on a system composed of five formamide molecules disposed in the same geometry as peptide bonds in an a helix. Charges variations are similar and suggest that FCPAC is suitable for quantitative estimation of cooperative effects. [less ▲]

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See detailNudging hair shedding by antidandruff shampoos. A comparison of 1% ketoconazole, 1% piroctone olamine and 1% zinc pyrithione formulations.
Franchimont, Claudine ULg; Goffin, Véronique ULg; Henry, Frédérique ULg et al

in International Journal of Cosmetic Science (2002), 24(5), 249-56

Hair shedding and hair thinning have been reported to be affected by dandruff and seborrhoeic dermatitis. The present study was conducted in 150 men presenting with telogen effluvium related to androgenic ... [more ▼]

Hair shedding and hair thinning have been reported to be affected by dandruff and seborrhoeic dermatitis. The present study was conducted in 150 men presenting with telogen effluvium related to androgenic alopecia associated with dandruff. They were randomly allocated to three groups receiving each one of the three shampoos in the market containing either 1% ketoconazole (KTZ), 1% piroctone olamine (PTO) or 1% zinc pyrithione (ZPT). Shampoos had to be used 2-3 times a week for 6 months. Hair shedding during shampoo was evaluated semiquantitatively. Hair density on the vertex was evaluated on photographs using a Dermaphot. Trichograms were used for determining the anagen hair percentage and the mean proximal hair shaft diameter using computerized image analysis. The sebum excretion rate (SER, mug cm(-2) h(-1)) was also measured using a Sebumeter((R)). The three treatments cleared pruritus and dandruff rapidly. At end point, hair density was unchanged, although hair shedding was decreased (KTZ: -17.3%, PTO: -16.5%, ZPT: -10.1%) and the anagen hair percentage was increased (KTZ: 4.9%, PTO: 7.9%, ZPT: 6.8%). The effect on the mean hair shaft diameter was contrasted between the three groups of volunteers (KTZ: 5.4%, PTO: 7.7%, ZPT: -2.2%). In conclusion, telogen effluvium was controlled by KTZ, PTO and ZPT shampoos at 1% concentration. In addition, KTZ and PTO increased the mean hair shaft thickness while discretely decreasing the sebum output at the skin surface. [less ▲]

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See detailLa vaccination contre la fièvre aphteuse : perspectives
Thiry, Etienne ULg; De Clercq, K.; Saegerman, Claude ULg et al

in Bulletin des Groupements Techniques Vétérinaires (2002), 14

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See detailAnalyse de l'impact physiologique et economique de l'elagage des arbres d'alignement en port libre.
Toussaint, André ULg; Kervyn de Meerendre, V.; Delcroix, B. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(2), 99-107

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See detailDetermination of affinity, stoichiometry and sequence selectivity of minor groove binder complexes with double-stranded oligodeoxynucleotides by electrospray ionization mass spectrometry
Rosu, Frédéric ULg; Gabelica, Valérie ULg; Houssier, Claude ULg et al

in Nucleic Acids Research (2002), 30(16), 82

Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove ... [more ▼]

Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove binders (Hoechst 33258, Hoechst 33342, DAPI, netropsin and berenil) and 12mer oligonucleotide duplexes with a central sequence (A/T)(4) flanked by G/C base pairs were chosen as model systems. To validate the electrospray ionization mass spectrometry (ESI-MS) method, comparisons were made with circular dichroism and fluorescence spectroscopy measurements. ESI-MS allowed the detection of minor (2 drug + DNA) species for Hoechst 33258, Hoechst 33342, DAPI and berenil with duplex d(GGGG(A/T)(4)GGGG). d(CCCC(A/T)(4)CCCC), which were undetectable with the other techniques. Assuming that the duplexes and the complexes have the same electrospray response factors, the equilbrium association constants of the 1:1 and 2:1 complexes were determined by ESI-MS, and the values show a good quantitative agreement with fluorescence determined constants for Hoechst 33258 and Hoechst 33342. It is also shown that ESI-MS can quickly give reliable information on the A/T sequence selectivity of a drug: the signal of a complex is directly related to the affinity of the drug for that particular duplex. The potential of ESI-MS as a qualitative and quantitative affinity screening method is emphasized. [less ▲]

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See detailKeratinocyte injury in drug-induced toxic epidermal necrolysis: Simultaneous but distinct topographic expression of CD95R and calprotectin.
Paquet, Philippe ULg; Pierard, Gérald ULg

in International Journal of Molecular Medicine (2002), 10(2), 145-7

Keratinocyte injury in drug-induced toxic epidermal necrolysis (TEN) is attributed to a dysregulation in the complex apoptotic machinery. This study was performed to investigate some aspects of the ... [more ▼]

Keratinocyte injury in drug-induced toxic epidermal necrolysis (TEN) is attributed to a dysregulation in the complex apoptotic machinery. This study was performed to investigate some aspects of the apoptotic pathomechanism involving calprotectin and the CD95 receptor (CD95R, FasR) in TEN. The expression of these two molecules corresponds to calcium-dependent and calcium-independent processes, respectively. Biopsies were collected from blistering skin in 21 TEN patients and from clinically uninvolved skin in 16 of these patients. Immunohistochemistry was performed using specific antibodies directed to CD95R and calprotectin. Half (8/16) of the biopsy specimens taken from clinically uninvolved sites showed the expression of calprotectin throughout the epidermis or restricted to the suprabasal layers. In these samples, a strong CD95R immunoreactivity was often restricted to the basal layer (8/16). Calprotectin was present in all biopsies of bullous skin, especially in suprabasal layers (15/21) or throughout the epidermis (6/21). By contrast, the prominent expression of CD95R was confined almost exclusively to the basal layer (15/21), and more rarely throughout the epidermis (2/21), and it remained sometimes unexpressed (4/21). A clear-cut boundary was often present between the areas labeled by the two antibodies with exceptional overlap between them. The simultaneous expression of calprotectin and CD95R in TEN at distinct levels of the epidermis indicates that the pathomechanism leading to keratinocyte death does not belong to a single process in TEN. Their expression in clinically uninvolved skin suggests that these processes are early and widespread events in TEN. [less ▲]

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See detailCaractérisation mécanique et chimique des surfaces et interfaces polymères par microscopies de force atomique
Cuenot, Stéphane; Duwez, Anne-Sophie ULg; Martin, Philippe et al

in Chimie Nouvelle (2002), 20

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See detailDetection Of Apple Chlorotic Leaf Spot Virus Using A 5 ' Nuclease Assay With A Fluorescent 3 ' Minor Groove Binder-Dna Probe
Vendrame, Marina; Kummert, Jean; Lepoivre, Philippe ULg et al

in Journal of Virological Methods (2002), 104(1), 99-106

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See detailEffects of incidental and intentional feature binding on recognition: a behavioural and PET activation study
Lekeu, Françoise ULg; Van der Linden, Martial ULg; Collette, Fabienne ULg et al

in Neuropsychologia (2002), 40(2), 131-144

Using Positron Emission Tomography (PET), we investigated cerebral regions associated with the episodic recognition of words alone and words bound to contextual colours. Two modes of colour encoding were ... [more ▼]

Using Positron Emission Tomography (PET), we investigated cerebral regions associated with the episodic recognition of words alone and words bound to contextual colours. Two modes of colour encoding were tested: incidental and intentional word-to-colour binding. Word-only recognition was associated with brain activation in a lexico-semantic left middle temporal region and in the cerebellum following an incidental colour encoding, and with brain activation in the left posterior middle frontal gyrus, right anterior cingulate and right inferior frontal gyrus following an intentional encoding. Recognition of bound features was associated with activation in left prefrontal and superior parietal regions following an incidental colour encoding, and with preferential right prefrontal cortex activation following an intentional colour encoding. Our results are in line with the hypothesis of a parietal involvement in context processing, and prefrontal areas in monitoring retrieval processes. Our results also support the hypothesis of a 'cortical asymmetry for reflective activity' (CARA). [less ▲]

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See detailLes bancs solaires au banc des accusés.
Uhoda, I.; Petite, Louicette ULg; Pierard, Claudine ULg et al

in Revue Médicale de Liège (2002), 57(1), 29-32

Sunbeds used in sun parlours do not garantee a controlled innocuity. By contrast, health hazards are obvious in many instances. Forceful tanning is a transitory aspect behind which the spectrum of many ... [more ▼]

Sunbeds used in sun parlours do not garantee a controlled innocuity. By contrast, health hazards are obvious in many instances. Forceful tanning is a transitory aspect behind which the spectrum of many skin changes includes accelerated photoageing, irreversible pigmentary and keratotic changes, and cutaneous malignancies including malignant melanoma. In the absence of protection, the eye is also affected. Even if tanning salon exposure increases the risk for skin cancer by a small amount, it represents a significant potential public health hazard given the number of people attending salons. Digital dermoscopy and ultraviolet light videoscopy are new methods allowing to predict the cutaneous ransom to be paid by the sunbed worshippers. [less ▲]

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See detailRéplication, clivage-encapsidation et recombinaison de l'ADN des herpèsvirus
Schynts, Frédéric; Meurens, François; Muylkens, Benoît ULg et al

in Virologie (2002), 6

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See detailJuvenile interphalangeal degenerative joint disease in the ardenner horse: morphometrical evaluation's protocol (part I)
Lejeune, Jean-Philippe ULg; Schneider, Nicole ULg; Duvivier, Dominique Hannia et al

in Annales de Médecine Vétérinaire (2002), 146(2, APR-MAY), 113-120

The observation of a high incidence of juvenile interphalangeal degenerative joint disease in ardenner horses motivated the constitution of a research project at the "Centre Europeen du Cheval de Mont-le ... [more ▼]

The observation of a high incidence of juvenile interphalangeal degenerative joint disease in ardenner horses motivated the constitution of a research project at the "Centre Europeen du Cheval de Mont-le-Soie" (Belgium). Its aim is to study the morphology of the horse, the conformation of its limbs as well as the potential outbreak and evolution of osteoarthritic lesions. Therefore an investigation method composed of morphometric and radiographic evaluation's protocols has been established. The morphometric evaluation's protocol presented here enables to characterise first the general morphology of the horse and second the foot, his asymmetries and his connections with the ground. This protocol is applied to 32 ardenner foals of 250 21 days of age. This group represents 9.3% of the births registered during the year 2000. For general morphometry, different values of the parameters are observed in this group. Only the higher size of the males makes the difference between the two populations (males and females) at this age. The morphometric study of the foot points out a widening of the external part of the hoof and an angle foot/ground too high in comparison with the established standards. Asymmetrical heights of hoof's quarters are also observed. [less ▲]

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See detailMurine 5T multiple myeloma cells induce angiogenesis in vitro and in vivo
Van Valckenborgh, E.; De Raeve, H.; Devy, L. et al

in British Journal of Cancer (2002), 86(5), 796-802

Multiple myeloma is a B cell malignancy. Recently, it has been demonstrated that bone marrow samples of patients with multiple myeloma display an enhanced angiogenesis. The mechanisms involved seem to be ... [more ▼]

Multiple myeloma is a B cell malignancy. Recently, it has been demonstrated that bone marrow samples of patients with multiple myeloma display an enhanced angiogenesis. The mechanisms involved seem to be multiple and complex. We here demonstrate that the murine 5T multiple myeloma models are able to induce angiogenesis in vitro by using a rat aortic ring assay and in vivo by determining the microvessel density. The rat aortic rings cultured in 5T multiple myeloma conditioned medium exhibit a higher number of longer and more branched microvessels than the rings cultured in control medium. In bone marrow samples from 5T multiple myeloma diseased mice, a statistically significant increase of the microvessel density was observed when compared to bone marrow samples from age-matched controls. The angiogenic phenotype of both 5T multiple myeloma cells could be related, at least in part, to their capacity to produce vascular endothelial growth factor. These data clearly demonstrate that the 5T multiple myeloma models are good models to study angiogenesis in multiple myeloma and will allow to unravel the mechanisms of neovascularisation, as well as to test new putative inhibitors of angiogenesis. [less ▲]

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See detailLymphocyte Kinetics: The Interpretation Of Labelling Data
Asquith, B.; Debacq, C.; Macallan, Dc. et al

in Trends In Immunology (2002), 23(12),

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See detailA simple approach for the design of steel and composite sway building frames
Maquoi, René ULg; Jaspart, Jean-Pierre ULg

in Steel Structures (2002), 2(n°1), 1-11

The Merchant-Rankine approach is a practical design procedure which allows to predict the ultimate resistance of sway steel building frames with rigid structural joints. In the present paper the ... [more ▼]

The Merchant-Rankine approach is a practical design procedure which allows to predict the ultimate resistance of sway steel building frames with rigid structural joints. In the present paper the background and the traditional field of application of the Merchant-Rankine formula are first described. Its extension to the design of steel frames with so-called semi-rigid joints is then presented and justified. Finally its application to steel-concrete sway composite building frames is investigated and first conclusions of this work are drawn. [less ▲]

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See detailLe miracle 'flamand' n'est pas prêt-à-porter…
Lafontaine, Dominique ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2002), 9-10

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See detailContribution à l'étude de la qualité bactériologique des eaux de surface à vocation touristique en Wallonie (Belgique)
Rosillon, Françis ULg; Culot, Marc ULg; Bosser, C. et al

in Cahiers de l'association scientifique européenne pour l'eau et la santé (2002), 7(1), 3-13

Au cours de la saison estivale 2000 et 2001, 33 zones récréatives en cours d'eau ont fait l'objet d'un suivi de la qualité bactériologique. Au-delà d'une simple surveillance, un profil de zone a été ... [more ▼]

Au cours de la saison estivale 2000 et 2001, 33 zones récréatives en cours d'eau ont fait l'objet d'un suivi de la qualité bactériologique. Au-delà d'une simple surveillance, un profil de zone a été établi. Celui-ci consiste à identifier et à hiérarchiser l'importance des sources de contamination fécale pouvant influencer la zone récréative. Lors d'événements pluvieux, une dégradation plus importante de la qualité des eaux a pu être mise en évidence. La diversité des sources de contamination implique une démarche multi-acteurs. A des actions ponctuelles de remédiation, s'ajoutent des mesures préventives d'accompagnement au sein de périmètres de protection. Une meilleure connaissance de la dynamique des germes fécaux dans le milieu naturel et du phénomène d'auto-épuration bactérienne devrait permettre de mieux définir les limites de ces périmètres de protection [less ▲]

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See detailMutations des localisations des services et mutations urbaines. Questionnements et perspectives
Merenne-Schoumaker, Bernadette ULg

in Revista de Geografia, Universidade Federal de Pernambuco (2002), 17(2), 41-56

The aim of this paper is to analyse the relationships between urban mutations and service locations. Work is developed in four times: facts, causes, consequences and prospects. Without doubt, the spatial ... [more ▼]

The aim of this paper is to analyse the relationships between urban mutations and service locations. Work is developed in four times: facts, causes, consequences and prospects. Without doubt, the spatial transfers of service activities can be considered as both, a cause and a consequence of urban sprawl. It is related to the release of mobility constraints, to the specific changes that affected many economic activities, and also to the new behaviours of many actors (customers/ visitors, property developers and public authorities). As this evolution is incompatible with the objectives of sustainable urban development, several action possibilities can be proposed: new management of the daily mobilities, reduction in space consumption and new governance. [less ▲]

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See detailThe role of the main silica precursor and the additive in the preparation, of low-density xerogels
Alié, Christelle ULg; Pirard, René ULg; Pirard, Jean-Paul ULg

in Journal of Non-Crystalline Solids (2002), 311(3), 304-313

The incorporation of an additive during sol-gel synthesis reduces shinkage during ambient drying. The following additives have been studied: 3-,(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3 ... [more ▼]

The incorporation of an additive during sol-gel synthesis reduces shinkage during ambient drying. The following additives have been studied: 3-,(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3-aminopropyltriethoxysilane (AES) and 3-(2-aminoethylamino)propyltriethoxysilane (EDAES) and the main silica precursors were tetraethylorthosilicate (TEOS) and tetrapropylorthosilicate (TPOS). When the additive contains methoxy groups (EDAS), it acts as a nucleation agent of the silica particles and exactly the same properties (pore volume, specific surface area, particle and aggregate size) are obtained whether the main reagent is TEOS or TPOS. The nucleation mechanism is based on the difference in reactivity between additive and main reagent. In case of nucleation by the additive, the nucleation agent fixes the properties whatever the main silica precursor is. When both the additive and the main reagent contain ethoxy groups (series AES-TEOS and EDAES-TEOS), there is no nucleation mechanism by the additive, and the silica particle size remains nearly constant. With less reactive main reagent (series AES-TPOS and EDAES-TPOS), pore volumes up to 17 cm(3)/g have been obtained with pore sizes up to nearly 10 pm and very big particles (similar to100 nm). The absence of nucleation by the additive for the couples AES-TPOS and EDAES-TPOS could be due to the fact that the difference in reactivity between ethoxy groups and propoxy groups is not sufficient to initiate the nucleation mechanism by the additive. In the absence of nucleation by the additive, the main reagent plays a role: highly porous materials with very large p. ores are prepared with TPOS. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailComment j'explore ... une métastase orpheline d'un mélanome.
Henry, Frédérique ULg; Pierard, Claudine ULg; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (2002), 57(6), 405-7

A presumed melanoma metastasis is sometimes found without any evidence for the primary neoplasm. Such an orphan metastasis merits a complementary exploration. Searching for the primary melanoma must then ... [more ▼]

A presumed melanoma metastasis is sometimes found without any evidence for the primary neoplasm. Such an orphan metastasis merits a complementary exploration. Searching for the primary melanoma must then be performed using non invasive and clinically oriented methods. Beside a thorough skin examination, otorhinolaryngological, ophtalmic and gynecologic searches are recommended. In addition, paraclinical investigations are conducted according to the metastatic location, the gender and the functional complaints of the patient. The prognosis of these orphan metastases is not more dramatic than when the primary lesion is identified. Therapies are similar to those proposed in the classical presentation of the disease. [less ▲]

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See detailEcology of Salmonella in slaughter pigs digestive tract and study of the contamination of carcasses
Boudry, Christelle ULg; Korsak Koulagenko, Nicolas ULg; Jacob, Benoit et al

in Annales de Médecine Vétérinaire (2002), 146(6, DEC-JAN), 353-360

This article presents a study on the Salmonella asymptomatic carriage of finishing pigs and its consequences on the contamination of carcasses. Twenty finishing pigs were followed to determine the faecal ... [more ▼]

This article presents a study on the Salmonella asymptomatic carriage of finishing pigs and its consequences on the contamination of carcasses. Twenty finishing pigs were followed to determine the faecal shedding of Salmonella. No sample was positive but at the slaughterhouse, the analysis of tonsils and certain parts of the digestive tract (mesenterial lymph nodes, ileum and large intestine content) revealed that 70% of the pigs were positive. The strains isolated from the faecal samples of the large intestine and from tonsils were identical, which might indicate that there has been an oral contamination from pig to pig due to the shedding of Salmonella or a carriage of different strains in different organs. The mesenterial lymph nodes (40 % positive) were contaminated by strains which were different from those from the faecal samples and tonsils. Salmonella strains were only found on the swabs of one carcass. A complementary assay has shown the negative role played by the scalding step. This latter may contaminate the oral and rectal cavities of slaughtered animals. This entire study reveals the difficulty to predict the contamination risk at the slaughterhouse from the analysis of finishing pigs faecal samples. Therefore, a better knowledge of the carriage and the shedding of Salmonella during the finishing period and during the slaughter is necessary to minimise meat contamination. [less ▲]

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See detailEuclidean and fractal computer-assisted corneofungimetry: a comparison of 2% ketoconazole and 1% terbinafine topical formulations.
Arrese Estrada, Jorge ULg; Fogouang, L.; Pierard, Claudine ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (2002), 204(3), 222-7

BACKGROUND: The corneofungimetry bioassay was designed as a unique model predicting the efficacy of topical and oral antifungals in dermatomycoses. OBJECTIVE: In this 2-step study performed in two groups ... [more ▼]

BACKGROUND: The corneofungimetry bioassay was designed as a unique model predicting the efficacy of topical and oral antifungals in dermatomycoses. OBJECTIVE: In this 2-step study performed in two groups of 15 volunteers, corneofungimetry was used to compare the effect of 5-day b.i.d. treatments with 2% ketoconazole and 1% terbinafine creams. METHODS: The bioassay was performed using 10 isolates of each of the 3 fungi Trichophyton rubrum, T. mentagrophytes var. interdigitale and Candida albicans put to grow on human stratum corneum. Controls were stratum corneum either untreated or enriched in propylene glycol contained in an unmedicated vehicle. Quantitative assessments were made using both Euclidean and fractal geometry parameters. RESULTS: In comparison with untreated stratum corneum, the fungitoxic activity of the 2% ketoconazole and 1% terbinafine formulations was obvious and similar against dermatophytes. By contrast, 2% ketoconazole was significantly more active against C. albicans than 1% terbinafine. The propylene-glycol-containing vehicle did not exhibit a significant effect upon the dermatophyte growth. Positive linear correlations were yielded between the extent area and the fractal dimension D of dermatophyte mycelia. By contrast, D appeared unrelated to the relative area of dermatophyte growth compared to controls. CONCLUSION: The combination of Euclidean and fractal analyses improves the information provided by the corneofungimetry bioassay. Creams containing 2% ketoconazole and 1% terbinafine appear equally effective against dermatophytes while the former is more potent against Candida albicans. [less ▲]

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See detailAn Immunologic investigation of canine eosinophilic bronchopneumopathy.
Clercx, Cécile ULg; Peeters, Dominique ULg; German, Alex et al

in Journal of Veterinary Internal Medicine (2002), 16

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See detailLes acquis des jeunes de 15 ans en sciences : des progrès restent à faire...
Lafontaine, Dominique ULg; Burton, Réginald

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2002), 9-10

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See detailGastric collision between a papillotubular adenocarcinoma and a gastrinoma in a patient with Zollinger-Ellison syndrome.
De Leval, Laurence ULg; Hardy, Noelle; Deprez, Manuel ULg et al

in Virchows Archiv : An International Journal of Pathology (2002), 441(5), 462-5

We report a unique case of a gastric collision tumor composed of an intramural gastrin-secreting tumor and a papillotubular adenocarcinoma of the intestinal type discovered at autopsy in a patient with ... [more ▼]

We report a unique case of a gastric collision tumor composed of an intramural gastrin-secreting tumor and a papillotubular adenocarcinoma of the intestinal type discovered at autopsy in a patient with Zollinger-Ellison syndrome. There was extensive metastatic dissemination of the neuroendocrine component to regional lymph nodes and to the liver. The unusual macroscopical, histological, and immunohistochemical features of this case and its specific clinical setting are discussed. [less ▲]

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See detailIsokinetic assessment of the forearm and wrist muscles
Forthomme, Bénédicte ULg; Croisier, Jean-Louis ULg; Foidart-Dessalle, Marguerite ULg et al

in Isokinetics & Exercise Science (2002), 10(3), 121-128

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See detailBiometrological assessment of skin protectors against moderate cold threat.
Claes, Geert; Pierard, Gérald ULg

in Exogenous Dermatology : Physical, Chemical, Biological (2002), 1

Background : Cold injuries represent a serious problem in winter sport practice and other outdoor activities. Some protection is expected to be achieved by specific topical preparations aiming at ... [more ▼]

Background : Cold injuries represent a serious problem in winter sport practice and other outdoor activities. Some protection is expected to be achieved by specific topical preparations aiming at decreasing heat loss by water evaporation from the skin. Objective : To compare the efficacy of 6 topical formulations enriched or not in rubefacients. The role of the vehicle was also assessed. Methods : A total of 30 trained cyclists tested the products on their forearms. The skin temperature and electrometric values were recorded at baseline following applications of the test products, and after 30 min-bicycle riding in moderately cold and humid outdoor conditions. Results : At baseline, skin temperature was increased by rubefacients dispersed a lipogel and o/w emulsion. This parameter decreased at all test sites after riding, but the values remained higher at the rubefacient-treated sites compared to the control untreated site. The oil and lipogel formulations achieved partial occlusion decreasing the transepidermal water loss. This effect was maintained after exercise without, however, hindering sweating. Conclusion : Among the presently tested formulations, the rubefacient-enriched lipogels appeared to be the most efficient formulations for maintaining skin temperature in the normal range during sport practice in a moderately cold environment. [less ▲]

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See detailEmbryo transfer as a method to eliminate pathogenic agents in a rabbit colony
Ectors, Fabien ULg; Delfosse, Henri; De Weder, L. et al

in Vlaams Diergeneeskundig Tijdschrift (2002), 71

To regain the SPF status of a contaminated but genetically valuable rabbit breeding unit, embryos from the contaminated does were transferred into SPF recipient females. Embryos were collected on day 3 of ... [more ▼]

To regain the SPF status of a contaminated but genetically valuable rabbit breeding unit, embryos from the contaminated does were transferred into SPF recipient females. Embryos were collected on day 3 of gestation by flushing uterine horns. All usable embryos were frozen, part of them were not transferred and kept in liquid nitrogen forming a stock of highly valuable genotypes. Thirty-two stimulated does produced 893 embryos, among which 821 (92%) had an intact zona pellucida and were cryopreserved. From this stock, 478 embryos were thawed, 466 were recovered (97.5%) and 417 were of good quality (87.2%). In 30 does, 10 to 18 embryos were surgically transferred per recipient doe and 24.9% (104/417) of them developed to term after transfer. This corresponds to an average number of 3.47 (104/30) live newborns per recipient. Health screenings performed on sanitized rabbits confirmed the disappearance of pathogenic agents. [less ▲]

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See detailObservations sur l'adage: Periculum est emptoris
Gerkens, Jean-François ULg

in Cahiers du Centre de Recherches en Histoire du Droit et des Institutions (2002), (17), 117-133

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See detailMagnetic and transport measurements on melt-textured DyBCO single domains
Vanderbemden, Philippe ULg; Misson, Vincent; Ausloos, Marcel ULg et al

in Physica C: Superconductivity (2002), 372(Part 2), 1225-1228

In this communication we report critical current measurements of melt processed DyBa2Cu3O7-x samples determined by several measuring techniques. First the material was characterized by AC susceptibility ... [more ▼]

In this communication we report critical current measurements of melt processed DyBa2Cu3O7-x samples determined by several measuring techniques. First the material was characterized by AC susceptibility and DC magnetization. The results are characteristic of good quality melt-processed (RE)BCO material with T-c = 89 K and Jc(77 K, 1 T) = 10^4 A/cm^2. Next, pulsed currents were used in order to determine both I-V curves and transport critical currents. The origin of the discrepancy between transport and magnetic data has been discussed. The results point out significant local variations of the critical current density throughout the single domain. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailAre central executive functions working in patients with focal frontal lesions?
Andrès Bénito, P.; Van der Linden, Martial ULg

in Neuropsychologia (2002), 40(7), 835-845

The aim of this study was to examine the hypothesis of a link between frontal cortex and two executive functions in working memory: the capacity to perform a dual task and the ability to inhibit ... [more ▼]

The aim of this study was to examine the hypothesis of a link between frontal cortex and two executive functions in working memory: the capacity to perform a dual task and the ability to inhibit irrelevant information. A dual task designed to assess the capacity to perform storage and processing simultaneously and a directed forgetting task designed to assess the capacity to actively inhibit no-longer relevant information were administered to a group of patients with focal frontal lesions and to a group of control participants. The results revealed that despite showing reduced short-term storage, frontal patients performed the dual task and inhibited the no-longer relevant information as well as control participants. These findings suggest that not all-executive processes are exclusively sustained by the frontal cortex [Quart J Exp Psychol 9 (1996) 5; Curr Opin Neurobiol 10 (2000) 195; Neuropsychology (1994) 544; The Cognitive Neuropsychology of Alzheimer-type dementia. Oxford, New York: Oxford University Press, 1996]. [less ▲]

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See detailSkin manifestations of Bartonella infections.
Chian, Cesar A; Arrese Estrada, Jorge ULg; Pierard, Gérald ULg

in International Journal of Dermatology (2002), 41(8), 461-6

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See detailLlandovery miospore biostratigraphy and stratigraphic evolution of the Parana Basin, Paraguay - Palaeogeographic implications
Steemans, Philippe ULg; Pereira, E.

in Bulletin de la Société Géologique de France (2002), 173(5), 407-414

The Parana Basin covers 1,600,000 km(2) including parts of southern Brazil, Paraguay, Uruguay and Argentina. It contains rocks ranging from the Ordovician to Tertiary in age. The present study is focused ... [more ▼]

The Parana Basin covers 1,600,000 km(2) including parts of southern Brazil, Paraguay, Uruguay and Argentina. It contains rocks ranging from the Ordovician to Tertiary in age. The present study is focused on the Itacurubi Group, of Llandovery age, from Paraguay. The sedimentology and palynology have been studied in three boreholes from the central part of Paraguay. The Itacurubi Group consists of the Euzebio Ayala, Vargas Pena and Cariy Formations. It corresponds to a complete transgressive - regressive cycle with maximum flooding in the Vargas Pena Formation. The sediments of the group were deposited in a glacio-marine regime and are included in the second order sequence. The palynofacies are dominated by acritarchs and chitinozoans, with miospores rare. Among the latter, cryptospores are most abundant and trilete spores very rare. The miospore assemblages are typical of the Llandovery. The presence of Laevolancis divellomedia and the successive first occurrence of the trilete spore genus Ambitisporites, followed by Archaeozonotriletes, allow the recognition of three biozones : divellomedia I, divellomedia II and chulus-nanus. Correlations between the three boreholes based on miospore biostratigraphy and sequence stratigraphy techniques are similar. The miospore assemblages have important palaeogeographic implications: (1) Ordovician / Silurian miospore assemblages, identified in South America are similar to those described in palaeogeographically distinct regions such as China, UK, Belgium, USA etc. This suggests that the phytogeographical differentiation proposed by Gray et al. [1992], with a Malvinokaffric Realm characterised by smooth tetrads and an extra-Malvinokaffric Realm characterised by ornamented tetrads, has to be reconsidered. Clearly, the same miospores are recovered from both regions, no matter if they are close to the palaeo-equator or the palaeo-pole, as is the case in Paraguay. These findings suggest that the same vegetation could survive under various climates. (2) In theory, large oceans could be impassable geographic barriers for land plant miospores larger than 25 mum in diameter. As similar miospore assemblages occur, around the Ordovician - Silurian boundary, on the Gondwana, Avalonia and Laurentia plates, it seems likely that these palaeo-continental plates were in close proximity permitting the expansion of the vegetation through these continents. Therefore, palaeogeographic reconstructions with narrow oceans between the continents better explain early miospore biogeographies in early Silurian times. (3) Maps produced by Dalziel et al. [1994] better explain the diachronism of trilete spore first appearances Hirnantian in Turkey, Rhuddanian in Saudi Arabia, early Aeronian in Libya, late Aeronian in Paraguay, latest Aeronian in UK, and possibly Telychian in USA. (4) The Baltica plate could possibly have been isolated by a geographic barrier during the Ashgill and the Llandovery, as only simple naked tetrads are known from that plate. The first appearance of the trilete spores in Gotland during the early Wenlock could correspond to the end of this geographic isolation. This apparent isolation of the Baltica plate could be due to a lack of data. [less ▲]

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