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See detailMulti-period stochastic optimization problems in transportation management (Ph. D. Thesis Summary)
Pironet, Thierry ULg

in 4OR : Quarterly Journal of the Belgian, French and Italian Operations Research Societies (2014)

Ph. D Thesis summary see other reference on ORBI

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See detailTowards understanding wild boar Sus scrofa movement: a synthetic movement ecology approach
Morelle, Kevin ULg; Podgórski, Tomasz; Prévot, Céline et al

in Mammal Review (2014)

In recent decades, the wild boar Sus scrofa has simultaneously increased its population size and colonized new habitats, causing more ecological and socio-economic concern than perhaps any other ungulate ... [more ▼]

In recent decades, the wild boar Sus scrofa has simultaneously increased its population size and colonized new habitats, causing more ecological and socio-economic concern than perhaps any other ungulate species. However, the drivers and mechanisms of the species' spatial ecology remain poorly understood. Thanks to a recently developed framework, the movement ecology of any organism can now be tackled within a consistent and unified theoretical approach. Based on this framework, we reviewed the literature on wild boar movement ecology to assess current knowledge and to identify important gaps. By using important navigational (e.g. olfactory sense) and cognitive (e.g. spatial memory, learning from conspecifics) abilities, wild boar have developed complex movement strategies to cope with external factors. However, there is a lack of detailed information on the role played by the internal state (motivation) and motion capacity in shaping the spatial ability of the species. Specific aspects of the movement ecology of the wild boar, together with its high diet plasticity and its high prolificacy, are probably the most important causes of the rapid spread of wild boar worldwide. We hope our review will inspire other scientists to apply their biological models to the movement ecology paradigms. Furthermore, we suggest that future researchers dealing with the movement ecology of any species should explicitly state the components and interactions of the framework investigated to facilitate further understanding and comparison among studies. [less ▲]

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See detailBlock, random and palm-tree amphiphilic fluorinated copolymers: controlled synthesis, surface activity and use as dispersion polymerization stabilizers
Alaimo, David ULg; Beigbeder, Alexandre; Dubois, Philippe et al

in Polymer Chemistry (2014), 5(18), 5273-5282

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See detailThermo-responsive gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) core–corona nanoparticles as a drug delivery system
Liu, Ji ULg; Detrembleur, Christophe ULg; Hurtgen, Marie et al

in Polymer Chemistry (2014), 5(18), 5289-5299

Core–corona gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) nanoparticles (gold@PVOH-b-PNVCL NPs) were fabricated via an in situ method, where a gold salt was reduced within the macromolecular aqueous ... [more ▼]

Core–corona gold/poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) nanoparticles (gold@PVOH-b-PNVCL NPs) were fabricated via an in situ method, where a gold salt was reduced within the macromolecular aqueous solution. Arrangement of macromolecular chains on the surface of gold cores was studied by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy and infrared spectroscopy. The responsiveness to temperature and the preserved colloidal stability of the gold@PVOH-b-PNVCL NPs above the lower critical solution temperature (LCST) were confirmed by dynamic light scattering and turbidity measurements. The drug loading capacity (DLC of ca. 1.3–2.8 wt%) of the gold@PVOH-b-PNVCL NPs as a drug delivery system (DDS) was tested with Nadolol®, a hydrophilic drug, and the release behaviours were studied at several temperatures. PVOH-b-PNVCL copolymers with an LCST of a few degrees above the biological temperature (37 °C), for example, PVOH180-b-PNVCL110 (LCST of 41 °C), are preferential, due to the slower release at 37 °C, but a faster release at temperatures that are a few degrees higher. The cytocompatibility of the gold@PVOH-b-PNVCL NPs against mouse fibroblastic L929 cells was evaluated via the MTS assay. Cellular uptake within MEL-5 human melanoma cells was studied by confocal laser scanning microscopy, fluorescence-activated cell sorting and TEM techniques and it showed that gold@PVOH-b-PNVCL NPs preferably accumulated within the cellular cytoplasm, with an incubation concentration and period-dependent uptake process. All these results corroborated a general utility of these thermo-responsive gold@PVOH-b-PNVCL NPs for drug delivery and controlled drug release. [less ▲]

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See detailChemical Mass Balance model applied to an olfactory annoyance problematic
Clarke, Kevin; Redon, Nathalie; Romain, Anne-Claude ULg et al

in Environmental Science & Technology (2014), Just accepted manuscript(web),

The source-receptor model CMB (Chemical Mass Balance) generally used for air pollution studies is applied for the first time to solve odour signatures issues. The olfactory annoyance perceived at a ... [more ▼]

The source-receptor model CMB (Chemical Mass Balance) generally used for air pollution studies is applied for the first time to solve odour signatures issues. The olfactory annoyance perceived at a receptor site of an industrial area is often the result of a combination of different smells, emitted by several industrial sources. To identify the main responsible for the inconvenience, tools able to determine the contribution of each source directly where the annoyance is perceived are widely developed and deployed. In this work, the contributions of VOC sources, coming from a waste treatment plant containing 3 potential sources of olfactory annoyance (waste storage, production of biogas, and compost piles of green wastes), and perceived at a village located downwind, are studied by chemical analyses. The CMB methodology is applied and the results are finally compared to olfactometric methods, in order to validate whether it is appropriate to use this model for olfactory problems or not. [less ▲]

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See detailMetabolic reprogramming in transformed mouse cortical astrocytes: a proteomic study.
Bentaib, Azeddine; De Tullio, Pascal ULg; Chneiweiss, Herve et al

in Journal of proteomics (2014)

Metabolic reprogramming is thought to play a key role in sustaining the survival and proliferation of cancer cells. These changes facilitate for example the uptake and release of nutrients required for ... [more ▼]

Metabolic reprogramming is thought to play a key role in sustaining the survival and proliferation of cancer cells. These changes facilitate for example the uptake and release of nutrients required for nucleotide, protein and lipid synthesis necessary for macromolecule assembly and tumor growth. We applied a 2D-DIGE (Two-Dimensional Differential in-Gel Electrophoresis) quantitative proteomic analysis to characterize the proteomes of mouse astrocytes that underwent in vitro cancerous transformation, and of their normal counterparts. Metabolic reprogramming effects on enzymatic and structural protein expression as well as associated metabolites abundance were quantified. Using enzymatic activity measurements and zymography, we documented and confirmed several changes in abundance and activity of various isoenzymes likely to participate in metabolic reprogramming. We found that after transformation, the cells increase their expression of glycolytic enzymes, thus augmenting their ability to use aerobic glycolysis (Warburg effect). An increased capacity to dispose of reducing equivalents through lactate production was also documented. Major effects on carbohydrates, amino acids and nucleotides metabolic enzymes were also observed. Conversely, the transformed cells reduced their enzymatic capacity for reactions of tricarboxylic acid oxidation, for neurotransmitter (glutamate) metabolism, for oxidative stress defense and their expression of astroglial markers. BIOLOGICAL SIGNIFICANCE: The use of a global approach based on a 2D DIGE analysis allows obtaining a comprehensive view of the metabolic reprogramming undergone by astrocytes upon cancerous transformation. Indeed, except for a few enzymes such as pyruvate carboxylase and glutaminase that were not detected in our initial analysis, pertinent information on the abundance of most enzymes belonging to pathways relevant to metabolic reprogramming was directly obtained. In this in vitro model, transformation causes major losses of astrocyte-specific proteins and functions and the acquisition of metabolic adaptations that favor intermediate metabolites production for increased macromolecule biosynthesis. Thus our approach appears to be readily applicable for the investigation of changes in protein abundance that determine various transformed cell phenotypes. It could similarly be applied to the evaluation of the effects of treatments aimed at correcting the consequences of cell transformation. [less ▲]

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See detailanxiety at the first radiotherapy session for non-metastatic brest cancer: key communication and communication-related predictors
Lewis, Florence; Merckaert, Isabelle; Liénard, Aurore et al

in Radiotherapy & Oncology (2014)

Background and purpose Patients may experience clinically relevant anxiety at their first radiotherapy (RT) sessions. To date, studies have not investigated during/around the RT simulation the key ... [more ▼]

Background and purpose Patients may experience clinically relevant anxiety at their first radiotherapy (RT) sessions. To date, studies have not investigated during/around the RT simulation the key communication and communication-related predictors of this clinically relevant anxiety. Material and methods Breast cancer patients (n = 227) completed visual analog scale (VAS) assessments of anxiety before and after their first RT sessions. Clinically relevant anxiety was defined as having pre- and post-first RT session VAS scores ⩾4 cm. Communication during RT simulation was assessed with content analysis software (LaComm), and communication-related variables around the RT simulation were assessed with questionnaires. Results Clinically relevant anxiety at the first RT session was predicted by lower self-efficacy to communicate with the RT team (OR = 0.65; p = 0.020), the perception of lower support received from the RT team (OR = 0.70; p = 0.020), lower knowledge of RT-associated side effects (OR = 0.95; p = 0.057), and higher use of emotion-focused coping (OR = 1.09; p = 0.013). Conclusions This study provides RT team members with information about potential communication strategies, which may be used to reduce patient anxiety at the first RT session. [less ▲]

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See detailInvestigating apical adverse effects of four endocrine active substances in the freshwater gastropod Lymnaea stagnalis
Giusti, Arnaud ULg; Lagadic, Laurent; Barsi, Alpar et al

in Science of the Total Environment (2014), 493

The hermaphroditic gastropod Lymnaea stagnalis is proposed as a candidate species for the development of OECD guidelines for testing of the reprotoxicity of chemicals, including endocrine active ... [more ▼]

The hermaphroditic gastropod Lymnaea stagnalis is proposed as a candidate species for the development of OECD guidelines for testing of the reprotoxicity of chemicals, including endocrine active substances (EASs). Up to now, only a few putative EASs have been tested for their reproductive toxicity in this species. In this study, we investigate the effects of four EASs with different affinities to the vertebrate estrogen and androgen receptors (chlordecone as an estrogen; cyproterone acetate, fenitrothion and vinclozolin as anti-androgens) on the reproduction of L. stagnalis in a 21-day semi-static test. Testosterone and 17α-ethinylestradiol (EE2) were used as the reference compounds. The tested EASs had no significant effect on growth and survival at the tested concentration ranges (ng to μg/L). Classical reproduction endpoints (i.e., oviposition and fecundity) were not responsive to the tested chemicals, except for chlordecone and 17α-ethinylestradiol, which hampered reproduction from 19.6 μg/L and 17.6 μg/L, respectively. The frequency of polyembryonic eggs, used as an additional endpoint, demonstrated the effects of all compounds except EE2. The molecular pathways, which are involved in such reproduction impairments, remain unknown. Our results suggest that egg quality is a more sensitive endpoint as compared to other reproductive endpoints commonly assessed in mollusk toxicity tests. [less ▲]

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See detailMultiqubit symmetric states with maximally mixed one-qubit reductions
Baguette, Dorian ULg; Bastin, Thierry ULg; Martin, John ULg

in Physical Review. A (2014), 90

We present a comprehensive study of maximally entangled symmetric states of arbitrary numbers of qubits in the sense of the maximal mixedness of the one-qubit reduced density operator. A general criterion ... [more ▼]

We present a comprehensive study of maximally entangled symmetric states of arbitrary numbers of qubits in the sense of the maximal mixedness of the one-qubit reduced density operator. A general criterion is provided to easily identify whether given symmetric states are maximally entangled in that respect or not. We show that these maximally entangled symmetric (MES) states are the only symmetric states for which the expectation value of the associated collective spin of the system vanishes, as well as in corollary the dipole moment of the Husimi function. We establish the link between this kind of maximal entanglement, the anticoherence properties of spin states, and the degree of polarization of light fields. We analyze the relationship between the MES states and the classes of states equivalent through stochastic local operations with classical communication (SLOCC). We provide a nonexistence criterion of MES states within SLOCC classes of qubit states and show in particular that the symmetric Dicke state SLOCC classes never contain such MES states, with the only exception of the balanced Dicke state class for even numbers of qubits. The 4-qubit system is analyzed exhaustively and all MES states of this system are identified and characterized. Finally the entanglement content of MES states is analyzed with respect to the geometric and barycentric measures of entanglement, as well as to the generalized N-tangle. We show that the geometric entanglement of MES states is ensured to be larger than or equal to 1/2, but also that MES states are not in general the symmetric states that maximize the investigated entanglement measures. [less ▲]

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See detailFundamental Limitations of High Contrast Imaging Set by Small Sample Statistics
Mawet, D.; Milli, J.; Wahhaj, Z. et al

in Astrophysical Journal (2014), 792

In this paper, we review the impact of small sample statistics on detection thresholds and corresponding confidence levels (CLs) in high-contrast imaging at small angles. When looking close to the star ... [more ▼]

In this paper, we review the impact of small sample statistics on detection thresholds and corresponding confidence levels (CLs) in high-contrast imaging at small angles. When looking close to the star, the number of resolution elements decreases rapidly toward small angles. This reduction of the number of degrees of freedom dramatically affects CLs and false alarm probabilities. Naively using the same ideal hypothesis and methods as for larger separations, which are well understood and commonly assume Gaussian noise, can yield up to one order of magnitude error in contrast estimations at fixed CL. The statistical penalty exponentially increases toward very small inner working angles. Even at 5-10 resolution elements from the star, false alarm probabilities can be significantly higher than expected. Here we present a rigorous statistical analysis that ensures robustness of the CL, but also imposes a substantial limitation on corresponding achievable detection limits (thus contrast) at small angles. This unavoidable fundamental statistical effect has a significant impact on current coronagraphic and future high-contrast imagers. Finally, the paper concludes with practical recommendations to account for small number statistics when computing the sensitivity to companions at small angles and when exploiting the results of direct imaging planet surveys. [less ▲]

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See detailAssessing the operation rules of a reservoir system based on a detailed modelling-chain
Bruwier, Martin ULg; Erpicum, Sébastien ULg; Pirotton, Michel ULg et al

in Natural Hazards & Earth System Sciences Discussion (2014), 2

According to available climate change scenarios for Belgium, drier summers and wetter winters are expected. In this study, we focus on two muti-purpose reservoirs located in the Vesdre catchment, which is ... [more ▼]

According to available climate change scenarios for Belgium, drier summers and wetter winters are expected. In this study, we focus on two muti-purpose reservoirs located in the Vesdre catchment, which is part of the Meuse basin. The current operation rules of the reservoirs are first analysed. Next, the impacts of two climate change scenarios are assessed and enhanced operation rules are proposed to mitigate these impacts. For this purpose, an integrated model of the catchment was used. It includes a hydrological model, one-dimensional and two-dimensional hydraulic models of the river and its main tributaries, a model of the reservoir system and a flood damage model. Five performance indicators of the reservoir system have been defined, reflecting its ability to provide sufficient drinking, to control floods, to produce hydropower and to reduce low-flow condition. As shown by the results, enhanced operation rules may improve the drinking water potential and the low-flow augmentation while the existing operation rules are efficient for flood control and for hydropower production. [less ▲]

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See detailThe PLATO 2.0 Mission
Rauer, H.; Catala, C.; Aerts, C. et al

in Experimental Astronomy (2014)

PLATO 2.0 has recently been selected for ESA’s M3 launch opportunity (2022/24). Providing accurate key planet parameters (radius, mass, density and age) in statistical numbers, it addresses fundamental ... [more ▼]

PLATO 2.0 has recently been selected for ESA’s M3 launch opportunity (2022/24). Providing accurate key planet parameters (radius, mass, density and age) in statistical numbers, it addresses fundamental questions such as: How do planetary systems form and evolve? Are there other systems with planets like ours, including potentially habitable planets? The PLATO 2.0 instrument consists of 34 small aperture telescopes (32 with 25 s readout cadence and 2 with 2.5 s candence) providing a wide field-of-view (2232 deg 2) and a large photometric magnitude range (4–16 mag). It focusses on bright (4–11 mag) stars in wide fields to detect and characterize planets down to Earth-size by photometric transits, whose masses can then be determined by ground-based radial-velocity follow-up measurements. Asteroseismology will be performed for these bright stars to obtain highly accurate stellar parameters, including masses and ages. The combination of bright targets and asteroseismology results in high accuracy for the bulk planet parameters: 2 %, 4–10 % and 10 % for planet radii, masses and ages, respectively. The planned baseline observing strategy includes two long pointings (2–3 years) to detect and bulk characterize planets reaching into the habitable zone (HZ) of solar-like stars and an additional step-and-stare phase to cover in total about 50 % of the sky. PLATO 2.0 will observe up to 1,000,000 stars and detect and characterize hundreds of small planets, and thousands of planets in the Neptune to gas giant regime out to the HZ. It will therefore provide the first large-scale catalogue of bulk characterized planets with accurate radii, masses, mean densities and ages. This catalogue will include terrestrial planets at intermediate orbital distances, where surface temperatures are moderate. Coverage of this parameter range with statistical numbers of bulk characterized planets is unique to PLATO 2.0. The PLATO 2.0 catalogue allows us to e.g.: - complete our knowledge of planet diversity for low-mass objects, - correlate the planet mean density-orbital distance distribution with predictions from planet formation theories,- constrain the influence of planet migration and scattering on the architecture of multiple systems, and - specify how planet and system parameters change with host star characteristics, such as type, metallicity and age. The catalogue will allow us to study planets and planetary systems at different evolutionary phases. It will further provide a census for small, low-mass planets. This will serve to identify objects which retained their primordial hydrogen atmosphere and in general the typical characteristics of planets in such low-mass, low-density range. Planets detected by PLATO 2.0 will orbit bright stars and many of them will be targets for future atmosphere spectroscopy exploring their atmosphere. Furthermore, the mission has the potential to detect exomoons, planetary rings, binary and Trojan planets. The planetary science possible with PLATO 2.0 is complemented by its impact on stellar and galactic science via asteroseismology as well as light curves of all kinds of variable stars, together with observations of stellar clusters of different ages. This will allow us to improve stellar models and study stellar activity. A large number of well-known ages from red giant stars will probe the structure and evolution of our Galaxy. Asteroseismic ages of bright stars for different phases of stellar evolution allow calibrating stellar age-rotation relationships. Together with the results of ESA’s Gaia mission, the results of PLATO 2.0 will provide a huge legacy to planetary, stellar and galactic science. [less ▲]

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See detailHow (not) to perform ecosystem service valuation - Pricing Gorillas in the Mist -
Boeraeve, Fanny ULg; Dendoncker, Nicolas; Jacobs, Sander et al

in Biodiversity and Conservation (2014)

Monetary valuation of ecosystem services (ES) is gaining growing interest in scientific papers, policies and awareness-raising documents for its potential as a communication tool illustrating the societal ... [more ▼]

Monetary valuation of ecosystem services (ES) is gaining growing interest in scientific papers, policies and awareness-raising documents for its potential as a communication tool illustrating the societal importance of biodiversity. However, simultaneously, its limitations are increasingly discussed in the literature. In this paper we argue that monetary valuation of ES should be seen as representing only one component of ES valuations. We provide basic standards to ensure integrated approaches to ES valuation that can effectively contribute to preserving cultural and biological diversity by acknowledging boundaries to resource exploitation and by building on the various interests and socio-cultural values of involved stakeholders. We base our discussion on a recent study that assesses the economic value of the world-famous Virunga National Park in the Democratic Republic of Congo, home to some of the last mountain gorillas (Gorilla beringei beringei). We alert against some ES monetary valuation that narrowly frames biodiversity conservation in terms of economic calculus and argue that subjugating conservation efforts to profit logics downplays the importance of intrinsic, symbolic and other non-economic values of biodiversity. We conclude by providing principles and methodological guidelines to enhance ES valuation as a tool to promote awareness rising for biodiversity conservation through the understanding the overall importance of biodiversity for human societies. [less ▲]

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See detailElastic damage to crack transition in a coupled non-local implicit discontinuous Galerkin/extrinsic cohesive law framework
Wu, Ling ULg; Becker, Gauthier ULg; Noels, Ludovic ULg

in Computer Methods in Applied Mechanics & Engineering (2014), 279

One current challenge related to computational fracture mechanics is the modeling of ductile fracture and in particular the damage to crack transition. On the one hand, continuum damage models, especially ... [more ▼]

One current challenge related to computational fracture mechanics is the modeling of ductile fracture and in particular the damage to crack transition. On the one hand, continuum damage models, especially in their non-local formulation which avoids the loss of solution uniqueness, can capture the material degradation process up to the localization of the damage, but are unable to represent a discontinuity in the structure. On the other hand cohesive zone methods can represent the process zone at the crack tip governing the crack propagation, but cannot account for the diffuse material damaging process. In this paper we propose to combine, in a small deformations setting, a non-local elastic damage model with a cohesive zone model. This combination is formulated within a discontinuous Galerkin nite element discretization. Indeed this DG weak formulation can easily be developed in a non-local implicit form and naturally embeds interface elements that can be used to integrate the traction separation law of the cohesive zone model. The method remains thus consistent and computationally e cient as compared to other cohesive element approaches. The effects of the damage to crack transition and of the mesh discretization are respectively studied on the compact tension specimen and on the double-notched specimen, demonstrating the efficiency and accuracy of the method. [less ▲]

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See detailDiscovery of a Companion Candidate in the HD 169142 Transition Disk and the Possibility of Multiple Planet Formation
Reggiani, Maddalena; Quanz, Sascha P.; Meyer, Michael R. et al

in Astrophysical Journal Letters (2014), 792

We present L'- and J-band high-contrast observations of HD 169142, obtained with the Very Large Telescope/NACO AGPM vector vortex coronagraph and the Gemini Planet Imager, respectively. A source located ... [more ▼]

We present L'- and J-band high-contrast observations of HD 169142, obtained with the Very Large Telescope/NACO AGPM vector vortex coronagraph and the Gemini Planet Imager, respectively. A source located at 0.''156 ± 0.''032 north of the host star (P.A. = 7.4° ± 11.3°) appears in the final reduced L' image. At the distance of the star (~145 pc), this angular separation corresponds to a physical separation of 22.7 ± 4.7 AU, locating the source within the recently resolved inner cavity of the transition disk. The source has a brightness of L' = 12.2 ± 0.5 mag, whereas it is not detected in the J band (J >13.8 mag). If its L' brightness arose solely from the photosphere of a companion and given the J - L' color constraints, it would correspond to a 28-32 MJup object at the age of the star, according to the COND models. Ongoing accretion activity of the star suggests, however, that gas is left in the inner disk cavity from which the companion could also be accreting. In this case, the object could be lower in mass and its luminosity enhanced by the accretion process and by a circumplanetary disk. A lower-mass object is more consistent with the observed cavity width. Finally, the observations enable us to place an upper limit on the L'-band flux of a second companion candidate orbiting in the disk annular gap at ~50 AU, as suggested by millimeter observations. If the second companion is also confirmed, HD 169142 might be forming a planetary system, with at least two companions opening gaps and possibly interacting with each other. [less ▲]

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See detailImmunovirologic Evaluation of Triomune (Lamivudine, Stavudine and Nevirapine) Antiretroviral Therapy in First Line HIV-1 Adult Patients in N’Djamena, Chad
Adawaye, Chatté Idekhim ULg

in Immunovirologic Evaluation of Triomune (Lamivudine, Stavudine and Nevirapine) Antiretroviral Therapy in First Line HIV-1 Adult Patients in N’Djamena, Chad (2014), 4

Dans cet article, nous avons évalué les taux des lymphocytes TCD4 et les charges virales chez des patients Vivant avec le VIH sous Triomune, à N'Djaména au Tchad. Cette évaluation a lieu au J0 avant le ... [more ▼]

Dans cet article, nous avons évalué les taux des lymphocytes TCD4 et les charges virales chez des patients Vivant avec le VIH sous Triomune, à N'Djaména au Tchad. Cette évaluation a lieu au J0 avant le traitement et après huit (8) mois de traitement. Nous avons remarqué que le taux d'échec à la Triomune était de 43,75% (21/48). [less ▲]

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See detailPlanets and Stellar Activity: Hide and Seek in the CoRoT-7 system
Haywood, R. D.; Cameron, A. C.; Queloz, D. et al

in Monthly Notices of the Royal Astronomical Society (2014), 443(3), 2517-2531

Since the discovery of the transiting Super-Earth CoRoT-7b, several investigations have been made of the number and precise masses of planets present in the system, but they all yield different results ... [more ▼]

Since the discovery of the transiting Super-Earth CoRoT-7b, several investigations have been made of the number and precise masses of planets present in the system, but they all yield different results, owing to the star's high level of activity. Radial velocity (RV) variations induced by stellar activity therefore need to be modelled and removed to allow a reliable detection of all planets in the system. We re-observed CoRoT-7 in January 2012 with both HARPS and the CoRoT satellite, so that we now have the benefit of simultaneous RV and photometric data. We fitted the off-transit variations in the CoRoT lightcurve using a harmonic decomposition similar to that implemented in Queloz et al. (2009). This fit was then used to model the stellar RV contribution, according to the methods described by Aigrain et al. (2011). This model was incorporated into a Monte Carlo Markov Chain in order to make a precise determination of the orbits of CoRoT-7b and CoRoT-7c. We also assess the evidence for the presence of one or two additional planetary companions. [less ▲]

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See detailDephosphorylated-uncarboxylated Matrix Gla protein concentration is predictive of vitamin K status and is correlated with vascular calcification in a cohort of hemodialysis patients.
DELANAYE, Pierre ULg; KRZESINSKI, Jean-Marie ULg; Warling, X et al

in BMC Nephrology (2014), 15

Background: Matrix Gla protein (MGP) is known to act as a potent local inhibitor of vascular calcifications. However, in order to be active, MGP must be phosphorylated and carboxylated, with this last ... [more ▼]

Background: Matrix Gla protein (MGP) is known to act as a potent local inhibitor of vascular calcifications. However, in order to be active, MGP must be phosphorylated and carboxylated, with this last process being dependent on vitamin K. The present study focused on the inactive form of MGP (dephosphorylated and uncarboxylated: dp-ucMGP) in a population of hemodialyzed (HD) patients. Results found in subjects being treated or not with vitamin K antagonist (VKA) were compared and the relationship between dp-ucMGP levels and the vascular calcification score were assessed. Methods: One hundred sixty prevalent HD patients were enrolled into this observational cohort study, including 23 who were receiving VKA treatment. The calcification score was determined (using the Kauppila method) and dp-ucMGP levels were measured using the automated iSYS method. Results: dp-ucMGP levels were much higher in patients being treated with VKA and little overlap was found with those not being treated (5604 [3758; 7836] vs. 1939 [1419; 2841] pmol/L, p <0.0001). In multivariate analysis, treatment with VKA was the most important variable explaining variation in dp-ucMGP levels even when adjusting for all other significant variables. In the 137 untreated patients, dp-ucMGP levels were significantly (p < 0.05) associated both in the uni- and multivariate analysis with age, body mass index, plasma levels of albumin, C-reactive protein, and FGF-23, and the vascular calcification score. Conclusion: We confirmed that the concentration of dp-ucMGP was higher in HD patients being treated with VKA. We observed a significant correlation between dp-ucMGP concentration and the calcification score. Our data support the theoretical role of MGP in the development of vascular calcifications. We confirmed the potential role of the inactive form of MGP in assessing the vitamin K status of the HD patients. [less ▲]

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See detailStand structure and species co-occurence in mixed and monodominant Central African tropical forests
Djuikouo, Marie Noël K.; Peh, Kelvin S.H.; Nguembou et al

in Journal of Tropical Ecology (2014), 30(5), 447-455

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See detailGabriel García Márquez herschrijft Columbus
Vanden Berghe, Kristine ULg

in Streven. Cultureel Maatschappelijk maandblad (2014), 81/8

In "De herfst van de patriarch" (1975) parodieert Gabriel García Márquez een fragment uit de logboeken van Columbus. In de parodie komen enkele thema's en stijlkenmerken naar voren die het oeuvre van de ... [more ▼]

In "De herfst van de patriarch" (1975) parodieert Gabriel García Márquez een fragment uit de logboeken van Columbus. In de parodie komen enkele thema's en stijlkenmerken naar voren die het oeuvre van de Colombiaanse schrijver ten volle typeren, zoals het idee dat de geschiedenis zich herhaalt, het rechtmatige streven van Latijns-Amerika naar culturele en politieke ontvoogding tegenover Spanje en de VS, en het gevoel voor humor van de auteur - ook wanneer hij het over dergelijke ernstige kwesties heeft. [less ▲]

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See detailLes drones au secours de la grande faune menacée de RDC
Linchant, Julie ULg; Lejeune, Philippe ULg; Vermeulen, Cédric ULg

in Parcs et Réserves (2014), 69(3), 5-13

Dans un contexte international de pressions de plus en plus intenses sur la faune sauvage, caractérisées notamment par une recrudescence intense du braconnage, le suivi régulier de la faune est essentiel ... [more ▼]

Dans un contexte international de pressions de plus en plus intenses sur la faune sauvage, caractérisées notamment par une recrudescence intense du braconnage, le suivi régulier de la faune est essentiel pour assurer sa conservation. La situation de la biodiversité en République Démocratique du Congo est particulièrement préoccupante suite aux nombreux conflits armés et aux pressions anthropiques qui en découlent, ainsi qu’au manque de moyens et d’investissement. Habituellement, le suivi de la faune est réalisé à l’aide d’inventaires pédestres et aériens. Cependant, ces méthodes font face à de nombreuses contraintes, notamment leur coût et la logistique importante qu’elles demandent. Elles sont aussi dangereuses pour les opérateurs, ces derniers risquant leur vie lors d’accidents de vol ou lors de rencontres avec des braconniers ou des animaux potentiellement dangereux. Dans ce contexte, l’utilisation des drones civils, actuellement en pleine expansion, représente une alternative aux méthodes classiques pour le suivi de la grande faune et la surveillance des aires protégées. En effet, cette nouvelle technologie présente un coût plus faible, une logistique facile et une prise en main rapide, ainsi qu’un minimum de risque pour les opérateurs. Néanmoins, elle en est à ses balbutiements et de nouvelles méthodes de suivi doivent être mises au point pour relever le défi imposé par les contraintes liées à ce matériel innovant, tels que la faible autonomie et le traitement de grands volumes de données. L’Université de Liège-Gembloux Agro-Bio Tech a bâti, en partenariat avec le CIFOR, le projet WiMUAS visant à développer ces nouvelles méthodes dans le cadre des aires protégées en RDC. Cet article s’appuie sur une revue bibliographique récente pour en présenter les trois principaux objectifs en regard des développements existants : - (i) la mise au point de nouvelles méthodes d’inventaire et de suivis ponctuels de la grande faune par analyse d’images et de vidéos obtenues par drone ; - (ii) la gestion des périphéries et activités illégales notamment grâce à la cartographie précise de ces activités qui peut être obtenue via l’imagerie drone ; - (iii) la lutte anti-braconnage qui pourrait être appuyée par une reconnaissance du terrain par drone pour rechercher et fournir des indices en limitant la mise en danger des gardes. [less ▲]

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See detailTranscranial magnetic stimulation combined with high density EEG in altered states of consciousness
Napolitani, M.; Bodart, Olivier ULg; Canali, P. et al

in Brain injury : [BI] (2014), 28(9), 1180-1189

Background: This review discusses the advantages of transcranial magnetic stimulation combined with high-density electroencephalography (TMS-hdEEG) over other current techniques of brain imaging. Methods ... [more ▼]

Background: This review discusses the advantages of transcranial magnetic stimulation combined with high-density electroencephalography (TMS-hdEEG) over other current techniques of brain imaging. Methods and results: Its application was reviewed, focusing particularly on disorders of consciousness, in the perspective of recent theories of consciousness. Assessment of non-communicative patients with disorders of consciousness remains a clinical challenge and objective measures of the level of consciousness are still needed. Current theories suggest that a key requirement for consciousness is the brain’s capacity to rapidly integrate information across different specialized cortical areas. TMS-EEG allows the stimulation of any given cortical area and the recording of the immediate electrical cortical response. This technique has recently been successfully employed to measure changes in brain complexity under physiological, pharmacological and pathological conditions. Conclusions: This suggests that TMS-EEG is a reliable tool to discriminate between conscious and unconscious patients at the single subject level. Future works are needed to validate and implement this technique as a clinical tool. [less ▲]

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See detailApproche prothétique rationnelle et conservatrice d'usure dentaire avancée
Vanheusden, Alain ULg

in Revue d'Odonto-Stomatologie [=ROS] (2014), 43(3), 251-268

For several years, the number of young adults consulting for tooth tissue loss of non carious origin has been significantly increasing. However, many practitioners are still reluctant to treat these ... [more ▼]

For several years, the number of young adults consulting for tooth tissue loss of non carious origin has been significantly increasing. However, many practitioners are still reluctant to treat these lesions, especially when they are severe. Nevertheless, thanks to the constant improvement of the protocols of enamel and dentin bonding and a greater expertise in the field of prosthetic biomaterials, some rational, reliable and minimally invasive therapeutic approaches can often be proposed. The purpose of this article is to describe, stage by stage, a protocol of treatment based on bonded partial prosthetic restorations, both on anterior and posterior teeth. [less ▲]

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See detailHammer head beam solution for beam-to-column joints in seismic resistant building frames
Hoang, Van Long ULg; Jaspart, Jean-Pierre ULg; Demonceau, Jean-François ULg

in Journal of Constructional Steel Research (2014)

This paper presents a research on an innovative stiffened extended end-plate joint, used to connect I-shaped beams to partially-encased composite wide flange columns. In the joint, T-shaped hammer heads ... [more ▼]

This paper presents a research on an innovative stiffened extended end-plate joint, used to connect I-shaped beams to partially-encased composite wide flange columns. In the joint, T-shaped hammer heads cut from the same I-profiles than the beams are used, instead of using traditional haunches. At the joint level, the column web is strengthened by two lateral plates welded to the column flanges; these plates also reinforce the column flanges. This type of joint is proposed to use in the seismic resistance building frames, as a full-strength and a fully-rigid joint solution. Firstly, a test program carried out within a RFCS European project titled HSS-SERF “High Strength Steel in Seismic Resistant Building Frames”, 2009–2013, will be presented. Then, analytical developments based on the component approach and aimed at predicting the joint response will be described; their validity will be demonstrated through comparisons with the tests. Moreover, a new design concept for full strength joint accounting for the actual position of the plastic hinge and the possible individual overstrength factors for each component is proposed, respecting the requirements of EN1998-1-1. [less ▲]

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See detailSynthesis, Surface characterization and photocatalytic activity of TiO2 supported on almond shell activated carbon
Omri, Abdessalem; Lambert, Stéphanie ULg; Geens, Jérémy ULg et al

in Journal of Materials Science & Technology (2014), 30(9), 894-902

Three types of photocatalysts were synthesized by metal organic chemical vapor deposition and impregnation methods using the almond shell activated carbon as support. These photocatalysts denoted by (TiO2 ... [more ▼]

Three types of photocatalysts were synthesized by metal organic chemical vapor deposition and impregnation methods using the almond shell activated carbon as support. These photocatalysts denoted by (TiO2/ASAC (V), TiO2/ASAC (I1) and TiO2/ASAC (I2)) were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and nitrogen adsorptionedesorption isotherms. SEM observation shows that TiO2 was deposited on activated carbon surface. XRD results confirm that TiO2 existed in a mixture of anatase and rutile phases. The DRS spectra show the characteristic absorption edge of TiO2 at approximate 380 nm corresponding to the optical band gap of 3.26 eV. Besides, FTIR spectrum indicated the presence of (TieO) groups. The specific surface area of photocatalysts decreased drastically in comparison with the original activated carbon. The catalysts were very efficient for the photodegradation of total organic carbon (TOC) from industrial phosphoric acid solution under UV irradiation. The kinetics of photocatalytic TOC degradation was found to follow a pseudofirst- order model. The prepared TiO2/ASAC showed high photoactivity for the photodegradation of TOC in the following order: TiO2/ASAC (V) > TiO2/ASAC (I1) > TiO2/ASAC (I2) > ASAC > TiO2 (P25). [less ▲]

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See detailBioaccumulation of polybrominated diphenyl ethers (PBDEs) in Gammarus pulex: Relative importance of different exposure routes and multipathway modeling
Lebrun, Jérémie; Leroy, Delphine; Giusti, Arnaud ULg et al

in Aquatic Toxicology (2014), 154

Characterizing the exposure routes of an organism and its ability to regulate accumulated contaminants is a crucial step toward developing a biomonitor. To date, very little data are available on the ... [more ▼]

Characterizing the exposure routes of an organism and its ability to regulate accumulated contaminants is a crucial step toward developing a biomonitor. To date, very little data are available on the bioaccumulation kinetics of PBDEs in freshwater biota. This study aims at investigating the potential use of a litter-degrader widely distributed in European freshwaters, Gammarus pulex, as an indicator of exposure to PBDEs. In aquatic microcosms, gammarids were exposed to a mixture of brominated congeners (BDE-28, 47, 66, 85, 99, 100, 153, 154 and 183) to assess their ability to bioconcentrate PBDEs. Results show that all tested congeners are highly internalized by G. pulex and uptake rates of PBDEs are closely related to their partition coefficients (Kow). The determination of the elimination rate of BDE-47, the congener most readily accumulated by gammarids, indicated that metabolism and excretion of this congener are low in G. pulex, which argues in favor of its use as a quantitative biomonitor. Finally, bioaccumulation experiments were performed using contaminated leaves to determine the relative importance of dietary uptake in the contamination of gammarids. Even though water is the preeminent exposure route, a significant uptake of BDE-47 through food was observed (27%). We propose a biodynamic model that takes into account both exposure routes to describe BDE-47 bioaccumulation. This study supports the use of this ubiquitous amphipod as an early warning monitor of the bioavailable contamination of freshwaters by PBDEs. [less ▲]

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See detailFraming and Political Discourse Analysis: Bush's Trip to Europe in 2005
Azpíroz Manero, María Luisa ULg

in Observatorio (OBS*) (2014), 8(3), 075-96

Framing empirical research analyzes the construction (frame building) and influence (frame effects) of the frames with which different social agents interpret and communicate reality. Based on Entman’s ... [more ▼]

Framing empirical research analyzes the construction (frame building) and influence (frame effects) of the frames with which different social agents interpret and communicate reality. Based on Entman’s (2004; 2008) and Kuypers’ (2008; 2009) contributions, this paper proposes and applies a qualitative methodology for the analysis of frame building in political discourse. It is located, therefore, on the strategic and methodological lines of framing research in political communication (Bartolomé; Rodríguez & Sádaba, 2012). As Kinder and Sanders state, frames are “invented and employed by political elites, often with an eye on advancing their own interests or ideologies, and intended to make favorable interpretations prevail” (Kinder & Sanders, 1990, p. 74). The paper includes a theoretical framework that reviews the evolution of framing literature in Communication, focusing especially on empirical studies. The contributions adopted for the elaboration of the methodology and the application process are explained in detail. The context of the case study, Bush’s discourse about the War on Terror on his trip to Europe in February 2005, is revised. Finally, the results obtained and the conclusions are detailed. [less ▲]

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See detailThe puzzling new class of variable stars in NGC 3766 : old friend pulsators?
Salmon, Sébastien ULg; Montalban Iglesias, Josefa ULg; Reese, Daniel et al

in Astronomy and Astrophysics (2014), 569

The recent variability survey of the NGC 3766 cluster revealed a considerable number of periodic variable stars in a region of the H-R diagram where no pulsation is expected. This region lies between the ... [more ▼]

The recent variability survey of the NGC 3766 cluster revealed a considerable number of periodic variable stars in a region of the H-R diagram where no pulsation is expected. This region lies between the instability strips of the delta Scuti and SPB stars. Moreover the periods of the new phenomenon, P~0.1-0.7 d, do not allow to associate it a priori to either of these two types of pulsations. Stars in the NGC 3766 cluster are known as fast rotators with rotational velocities typically larger than half of their critical velocity. Rotation can affect both the geometrical properties and period domain of pulsations. It also alters the apparent stellar luminosity through gravity darkening, effect seldom taken considered in theoretical studies of the rotation-pulsation interaction. We explore if both of these effects are able to deliver a consistent interpretation for the observed properties of the "new variables" in NGC 3766: explaining their presence outside the known instability strips and their variability periods. We carry out an instability analysis of SPB models within the framework of the Traditional Approximation of Rotation and study the visibility of modes according to the angle of view and rotation. We also check how gravity darkening affects the effective temperature and luminosity of stellar models for different angles of view and rotation velocities. At the red (cold) border of the instability strip, prograde sectoral modes are preferentially excited and their visibilities are maximum when seen equator-on. Furthermore low-mass SPB models seen equator-on can appear in the gap between non-rotating SPB and delta Scuti stars due to gravity darkening. In that case, periods of these most visible modes are shifted to the 0.2-0.5 d range due to the effects of the Coriolis force. We hence suggest that the new variable stars observed in NGC 3766 are actually fast rotating SPB pulsators. [less ▲]

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See detailMinerals and trace elements in traditional foods of rural areas of Lhasa Prefecture, Tibet Autonomous Region (P.R. China)
DERMIENCE, Michael ULg; Li, Xiao Wei; Mathieu, Françoise et al

in Journal of Food Composition and Analysis (2014), 35(2),

Traditional foods play a major role in the diet of rural people living in the Tibet Autonomous Region of the People's Republic of China. Because these foods are mainly derived from local agriculture as ... [more ▼]

Traditional foods play a major role in the diet of rural people living in the Tibet Autonomous Region of the People's Republic of China. Because these foods are mainly derived from local agriculture as well as artisanal production, their mineral composition may show significant discrepancies when compared with food composition data. This study aims at providing relevant data on the mineral composition of the main Tibetan foods. Sixteen different foodstuffs were sampled, including water, concentrated brewed black tea, chang, tsampa, wheat flour, dried cheese, dried yak meat, dried mutton, blood sausage, dried wild peaches, dried Chinese radish, and dried nettles. They were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) for 19 minerals: Na, K, P, Ca, Mg, Fe, Zn, Mn, Cu, Ni, Se, Mo, Al, As, Cr, Co, Cd, Pb, and V. The validity of the results was ensured by the use of standard reference materials. A statistical comparison of the mean mineral contents of the analyzed foods against food composition data from the China Food Composition (CFC) table was carried out. It revealed significant discrepancies, emphasizing the importance of food analysis for nutritional assessment in Tibet autonomous region. To the best of our knowledge, the mineral compositions of some traditional Tibetan foods are here reported for the first time. [less ▲]

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See detailMode of Action of Lactoperoxidase as Related to Its Antimicrobial Activity: A Review
Bafort, Françoise ULg; Parisi, Olivier ULg; Perraudin, Jean-Paul et al

in Enzyme Research (2014), 2014

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See detail" PIONEERS of the BLUES REVIVAL" by Steve CUSHING
Sacré, Robert ULg

in ABS Magazine (2014), (n° 43),

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See detailOn the Elasto-viscoplastic behavior of the Ti5553 alloy
ben bettaieb, Mohamed; Van Hoof, Thibaut; Pardoen, thomas et al

in Materials Science & Engineering : A (2014), 617

The elastoviscoplastic behavior of the Ti533 alloy is characterized and compared to the classical Ti-6A14V alloy. The true stress-strain curves are determined based on tensile tests performes under ... [more ▼]

The elastoviscoplastic behavior of the Ti533 alloy is characterized and compared to the classical Ti-6A14V alloy. The true stress-strain curves are determined based on tensile tests performes under different strain rates at room temperature and at 150°C, from which the elastic constants and the parameters of a Norton-Hoff viscoplastic model are identified. The strength of the Ti5553 alloy is 20-40% higherthan the strength of the Ti-6A1-4V alloy. The Ti5553 alloy constitutes thus a promising candidate for advanced structural applications. In view of modeling structural applications of forming operations, the elastic and plastic initial anisotropy of the two alloys is investigated by combining compression on cylinders with elliptical sections, uniaxial tensile tests in different material directions , plane strain and shear tests. The initial anisotropy of the different alloys is very weak which justifies the modeling of the mechanical behavior with isotropic yield surface. The identified elastoviscoplastic model is validated by comparing experimental results with FE predictions both on cylindrical specimens subjected to tensile tests and on flat specimens subjected to plane strain conditions. [less ▲]

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See detailFrom fair milk to fair enterprise: the consequences of an unexpected "bricolage"
Feyereisen, Marlène ULg; Melard, François ULg

in Outlook on Agriculture (2014), 43(3), 207-211

In recent years, the European Union and the Common Agricultural Policy have made considerable changes in policy related to the dairy sector. After two successive dairy crises in 2009 and 2012, the dairy ... [more ▼]

In recent years, the European Union and the Common Agricultural Policy have made considerable changes in policy related to the dairy sector. After two successive dairy crises in 2009 and 2012, the dairy sector in Belgium was weakened considerably. The Belgian fair-trade milk enterprise, Fairebel, was created by dairy farmers a few months after the 2009 European milk crisis, seeking to develop their alternative, supported, owned by and for traditional dairy farmers. This case study brings into question ‘fair trade’ as a concept and practice in terms of trade between enterprises in Europe, and the surprise effects of interaction when analysed at both European and regional scales. The study concludes that the best innovation processes are not necessarily radical, and that tinkering – or bricolage – is the path taken by many innovations. [less ▲]

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See detailMathematical modeling of HIV dynamics after antiretroviral therapy initiation: A clinical research study
Moog, Claude; Rivadeneira, Pablo; Stan, Guy-Bart et al

in AIDS Research and Human Retroviruses (2014), 30(9), 831-834

Immunological failure is identified from the estimation of certain parameters of a mathematical model of the HIV infection dynamics. This identification is supported by clinical research results from an ... [more ▼]

Immunological failure is identified from the estimation of certain parameters of a mathematical model of the HIV infection dynamics. This identification is supported by clinical research results from an original clinical trial. Standard clinical data were collected from infected patients starting Highly Active Anti-Retroviral Therapy (HAART), just after one month following therapy initiation and were used to carry out the model identification. The early diagnosis is shown to be consistent with the patients monitoring after six months. [less ▲]

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See detailInterview STARS OF HEAVEN
Sacré, Robert ULg

in ABS Magazine (2014), (n° 43),

Interview avec photos

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See detailSTARS of HEAVEN - interview ( Chicago, Juin 2014)
Sacré, Robert ULg

in ABS Magazine (2014), (n° 43), 28-32

Interview des membres des Stars of Heaven ; photos

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See detailA two-level emergency control scheme against power system voltage instability
Otomega, Bogdan; Glavic, Mevludin ULg; Van Cutsem, Thierry ULg

in Control Engineering Practice (2014), 30

A two-level adaptive control scheme against power system voltage instability is proposed, to deal with emergency conditions by acting on distribution transformers and/or by curtailing some loads. The ... [more ▼]

A two-level adaptive control scheme against power system voltage instability is proposed, to deal with emergency conditions by acting on distribution transformers and/or by curtailing some loads. The lower level includes distributed controllers, each acting once the voltage at a monitored transmission bus settles below a threshold value. The upper level benefits from wide-area monitoring and adjusts in real-time the voltage thresholds of the local controllers. Emergency detection is based on the sign of sensitivities. The proposed scheme is robust with respect to communication failures. Its performance is illustrated through detailed simulations of a small but realistic 74-bus test system. [less ▲]

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See detailPhysical Therapists’ Knowledge, Attitudes, and Beliefs about Physical Activity: A Prerequisite to their Role in Physical Activity Promotion?
Mouton, Alexandre ULg; MUGNIER, Benoît ULg; Demoulin, Christophe ULg et al

in Journal of Physical Therapy Education (2014), 28(3), 120-127

Background. The promotion of physical activity (PA) is widely recognized as a major component of the prevention and management of several chronic diseases. As experts in human movement, physical ... [more ▼]

Background. The promotion of physical activity (PA) is widely recognized as a major component of the prevention and management of several chronic diseases. As experts in human movement, physical therapists (PTs) are logical agents for the promotion of PA. Nevertheless, to date only few studies have investigated factors that influence PA promotion. Objectives. This study aimed to analyze the relationships between physical therapist knowledge, attitudes, and beliefs about PA, and their own selfreported PA promotion. Design. A sample of 185 PTs (male = 102) completed a cross-sectional survey through an online questionnaire. It included 25 multiple choice, Likert scale, and open-ended questions related to (1) personal characteristics, (2) knowledge, attitudes, and beliefs about PA, and (3) self-reported PA promotion by PTs. A deductive and inductive content analysis of the openended questions was also performed. Results. The questionnaire revealed that 99% of PTs agreed (n = 30) or completely agreed (n = 154) with the fact that they have to contribute to PA promotion in their patients. Significant correlations were found between physical therapist knowledge of the definition of PA, the benefits of PA, and their self-reported PA promotion (0.251, P < .01 and 0.180, P < .05, respectively). Additional multivariate analysis of the predictors of the self-reported PA promotion exposed significant univariate effects of knowledge, attitudes, and beliefs about PA (P < .01). Conclusion. Although the role of PTs in PA promotion seems evident, this survey underlines important misconceptions about PA within this group of health care professionals. Significant relationships between physical therapist knowledge, attitudes, and beliefs about PA and self reported promotion of PA highlight the need for physical therapist education programs to emphasize teaching the fundamentals of PA. [less ▲]

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See detailInter-method variability in bone alkaline phosphatase measurement : clinical impact on the management of dialysis patients
CAVALIER, Etienne ULg; Souberbielle, Jean-Claude; GADISSEUR, Romy ULg et al

in Clinical Biochemistry (2014), 47(13-14), 1227-30

BACKGROUND: Bone-specific alkaline phosphatase (BAP) is now recommended to assess bone turnover in hemodialysis (HD) patients. However, little is known about potential variability between methods ... [more ▼]

BACKGROUND: Bone-specific alkaline phosphatase (BAP) is now recommended to assess bone turnover in hemodialysis (HD) patients. However, little is known about potential variability between methods available to measure BAP. METHODS: We measured BAP in 76 HD patients with six different assays (Beckman-Coulter Ostase IRMA, Beckman-Coulter Ostase Access, IDS iSYS Ostase, IDS Ostase enzyme immunoassay, DiaSorin Liaison Ostase and Quidel MicroVue BAP). RESULTS: We observed a high correlation between all the assays ranging from 0.9948 (IDS iSYS vs. IDS EIA) to 0.9215 (DiaSorin Liaison vs. Quidel MicroVue). However, using the regression equations, the equivalent concentration of a Beckman-Coulter Access value of 10μg/L can range from 7.7 to 14.4μg/L and of 20μg/L can range from 16.9 to 27.9μg/L with other assays. According to Beckman-Coulter Access, 13%, 50% and 37% of the patients presented BAP values ≤10, between 10 and 20 and ≥20μg/L, respectively. Discrepancies are observed when other assays are used (concordance from 10 to 100%). CONCLUSIONS: Analytical problems leading to inter-method variation should be overcome to improve the usefulness of this marker in clinical practice. According to correlation results, recalibration of BAP assays is necessary but should not be a major issue. [less ▲]

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See detailMémoire épisodique dans la maladie d’Alzheimer : Déclin de la remémoration consciente et de la familiarité ?
Simon, Jessica ULg; Bastin, Christine ULg

in Medecine Sciences : M/S (2014), 30

According to the dual-process recognition models, the retrieval of information from long-term memory is supported by recollection and familiarity. Alzheimer’s disease is consistently found to affect ... [more ▼]

According to the dual-process recognition models, the retrieval of information from long-term memory is supported by recollection and familiarity. Alzheimer’s disease is consistently found to affect recollection. As for familiarity, however, no consensus has been reached so far. Some studies are in favor of an early impairment of familiarity, while others are in favor of a preservation of familiarity in the mild and moderate stages of the disease. This lack of consensus can be partly explained by methodological differences between studies. We discuss three methodological dimensions: the type of recognition test, the type of paradigm for estimating familiarity and recollection, and the nature of the material used. These differences reveal the complex nature of familiarity which would be underlied by different mechanisms that may be selectively altered or preserved in the disease. [less ▲]

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See detailConsommation de produits d'origine animale dans la concession forestière 039/11 de la SODEFOR à Oshwe (R.D. Congo)
Semeki Ngabinzeke, Jean; Belani Masamba, Justin; Ntoto M'Vubu et al

in Tropicultura (2014), 32(3), 147-155

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See detailComentário sobre o artigo de Pizzanelli
Jamoulle, Marc ULg

in Revista Brasileira de Medicina de Família e Comunidade (2014), 9(31),

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See detailMultilocus sequence typing analysis and antibiotic resistance of Clostridium difficile strains isolated from retail meat and humans in Belgium
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Avesani, Véronique et al

in Food Microbiology (2014), 42

Clostridium difficile has been isolated from food animals and meat, specially ground pork and ground beef. The recovered isolates were closely related to C. difficile human strains, indicating that ... [more ▼]

Clostridium difficile has been isolated from food animals and meat, specially ground pork and ground beef. The recovered isolates were closely related to C. difficile human strains, indicating that animals and food are possible transmission routes of human C. difficile infection. The main objective of this study was to characterize C. difficile isolates from retail meat and to compare with human isolates recovered from hospital patients in Belgium. Raw meat (beef and pork) was obtained from the retail trade. C. difficile was recovered from 2.3% of the beef samples and from 4.7% of the pork samples. A total of 4 different PCR-ribotypes were identified with a large percentage of types 078 and 014. Resistance to moxifloxacin and erythromycin was detected. The multi-locus sequence typing (MLST) analysis showed that meat and human isolates cluster in the same lineage. This study reveals the presence of toxigenic C. difficile in retail meat in Belgium with predominance PCR-ribotypes 078 and 014, which are among the four most prevalent ribotypes of C. difficile isolated from humans in Europe. [less ▲]

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See detailControllable pulse parameter transcranial magnetic stimulator with enhanced circuit topology and pulse shaping
Peterchev, Angel; D'Ostilio, Kevin ULg; Rothwell, John et al

in Journal of Neural Engineering (2014), 11(5),

Abstract. Objective. This work aims at flexible and practical pulse parameter control in transcranial magnetic stimulation (TMS), which is currently very limited in commercial devices. Approach. We ... [more ▼]

Abstract. Objective. This work aims at flexible and practical pulse parameter control in transcranial magnetic stimulation (TMS), which is currently very limited in commercial devices. Approach. We present a third generation controllable pulse parameter device (cTMS3) that uses a novel circuit topology with two energy-storage capacitors. It incorporates several implementation and functionality advantages over conventional TMS devices and other devices with advanced pulse shape control. cTMS3 generates lower internal voltage differences and is implemented with transistors with lower voltage rating than prior cTMS devices. Main results. cTMS3 provides more flexible pulse shaping since the circuit topology allows four coil-voltage levels during a pulse, including approximately zero voltage. The nearzero coil voltage enables snubbing of the ringing at the end of the pulse without the need for a separate active snubber circuit. cTMS3 can generate powerful rapid pulse sequences (< 10 ms inter pulse interval) by increasing the width of each subsequent pulse and utilizing the large capacitor energy storage, allowing the implementation of paradigms such as paired-pulse and quadripulse TMS with a single pulse generation circuit. cTMS3 can also generate theta (50 Hz) burst stimulation with predominantly unidirectional electric field pulses. The cTMS3 device functionality and output strength are illustrated with electrical output measurements as well as a study of the effect of pulse width and polarity on the active motor threshold in 10 healthy volunteers. Significance. The cTMS3 features could extend the utility of TMS as a research, diagnostic, and therapeutic tool. [less ▲]

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See detailBiointerface multiparametric investigation of intraocular lens acrylic materials
Bertrand, Virginie ULg; Bozukova, Dimitriya; Svaldo Lanero, Tiziana et al

in Journal of Cataract & Refractive Surgery (2014), 40(9), 1536-1544

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See detailA Geometrical Acoustics Approach Linking Surface Scattering and Reverberation in Room Acoustics
Embrechts, Jean-Jacques ULg

in Acta Acustica United with Acustica (2014), 100(5), 864-879

A general model of the influence of surface scattering on the reverberation time would have several applications in room acoustics. Such a model is not yet available, and it is the purpose of this paper ... [more ▼]

A general model of the influence of surface scattering on the reverberation time would have several applications in room acoustics. Such a model is not yet available, and it is the purpose of this paper to investigate a geometrical acoustics approach. Starting from the radiative transfer equation, an exponential solution is first developed for the reverberation energy decay in rooms with diffusely reflecting boundaries. Differences with the diffuse sound field (Sabine) theory are highlighted, leading to a modified formula for the reverberation time which is shown to be more in accordance with ray tracing simulations. A general model is then proposed for rooms in which specular and diffuse reflections coexist. This general model is applied in rooms where the specular contribution can be assumed to be quasi-isotropic and uniform. Under this assumption, the reverberation decay is represented by the sum of two exponential functions, depending on the scattering coefficients. However, it is shown that the influence of surfaces’ scattering on reverberation is rather limited in this case. On the contrary, rooms with a pair of parallel surfaces are prone to create significant anisotropy in the cloud of image sources. An analytical formulation is proposed in this case for the specular and diffuse contributions, provided that some assumptions are again made on the specularly reflected sound field. The final expression is not really intuitive concerning the relation between scattering coefficients and reverberation, but it contains all the variables influencing this relation. This allows fast evaluations of the effect of surface scattering in particular situations. Finally, the application of this model to room acoustics computer simulations is illustrated by an example. [less ▲]

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See detailLa stratégie chinoise en Amérique latine : entre coopération et hégémonie. Le cas du Brésil
Wintgens, Sophie ULg

in Revue de la Faculté de Droit de l'Université de Liège (2014), 59(2), 301-336

Cet article part du constat que la Chine a fortement accru sa présence en Amérique latine durant la dernière décennie. Il observe plus précisément que les pays avec lesquels elle a rapidement augmenté ses ... [more ▼]

Cet article part du constat que la Chine a fortement accru sa présence en Amérique latine durant la dernière décennie. Il observe plus précisément que les pays avec lesquels elle a rapidement augmenté ses échanges économiques et commerciaux se sont également engagés dans de nouveaux schémas de coopération et partenariat, posant la question de la Chine comme potentielle « alternative » aux partenaires occidentaux traditionnels de ce sous-continent. Là où nombre d’études s’interrogent sur l’impact des relations sino-latino-américaines, celle-ci propose d’analyser la stratégie chinoise envers l’Amérique latine, en s’appuyant sur le cas du partenariat stratégique avec le Brésil. Dans ce cadre, nous postulons que l’accroissement de l’influence de la Chine dans cette région du monde ne dépend pas seulement de la disponibilité de ses ressources (économiques, politiques, culturelles, militaires, etc.), ni de son aptitude à les mobiliser adéquatement, mais qu’elle tient également à la représentation qu’elle véhicule en tant qu’acteur mondial (coopération Sud-Sud) ainsi qu’à sa reconnaissance par les autres acteurs du système international. En ce sens, la puissance de la Chine se mesure également à travers sa capacité à s’affirmer comme une référence pour ses pairs émergents, voire auprès des puissances occidentales. En appréhendant les différentes dimensions de la puissance chinoise à travers ses actions de politique extérieure et les discours afférents, nous mesurerons dès lors la capacité d’influence de la Chine en Amérique latine, et plus précisément vis-à-vis du Brésil, à travers l’écart entre son discours (coopération) et la réalité (hégémonie). [less ▲]

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See detailOn the role of pressure in elasto-inertial turbulence
Terrapon, Vincent ULg; Dubief, Yves; Soria, Julio

in Journal of Turbulence (2014), 16(1), 26-43

The dynamics of elasto-inertial turbulence is investigated numerically from the perspective of the coupling between polymer dynamics and flow structures. In particular, direct numerical simulations of ... [more ▼]

The dynamics of elasto-inertial turbulence is investigated numerically from the perspective of the coupling between polymer dynamics and flow structures. In particular, direct numerical simulations of channel flow with Reynolds numbers ranging from 1000 to 6000 are used to study the formation and dynamics of elastic instabilities and their effects on the flow. Based on the splitting of the pressure into inertial and polymeric contributions, it is shown that the polymeric pressure is a non-negligible component of the total pressure fluctuations, although the rapid inertial part dominates. Unlike Newtonian flows, the slow inertial part is almost negligible in elasto-inertial turbulence. Statistics on the different terms of the Reynolds stress transport equation also illustrate the energy transfers between polymers and turbulence and the redistributive role of pressure. Finally, the trains of cylindrical structures around sheets of high polymer extension that are characteristics of elasto-inertial turbulence are shown to be correlated with the polymeric pressure fluctuations. [less ▲]

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See detailLower critical field and SNS-Andreev spectroscopy of 122-arsenides: Evidence of nodeless superconducting gap
Abdel-Hafiez, M.; Pereira, P.J.; Kuzmichev, S.A. et al

in Physical Review. B : Condensed Matter (2014), 90

Using two experimental techniques, we studied single crystals of the 122-FeAs family with almost the same critical temperature, Tc. We investigated the temperature dependence of the lower critical field ... [more ▼]

Using two experimental techniques, we studied single crystals of the 122-FeAs family with almost the same critical temperature, Tc. We investigated the temperature dependence of the lower critical field Hc1(T) of a Ca0.32Na0.68Fe2As2 (Tc ≈ 34 K) single crystal under static magnetic fields H parallel to the c axis. The temperature dependence of the London penetration depth can be described equally well either by a single anisotropic s-wave-like gap or by a two-gap model, while a d-wave approach cannot be used to fit the London penetration depth data. Intrinsic multiple Andreev reflection effect spectroscopy was used to detect bulk gap values in single crystals of the intimate compound Ba0.65K0.35Fe2As2, with the same Tc. We estimated the range of the large gap value L = 6–8 meV (depending on small variation of Tc) and its a k space anisotropy of about 30%, and the small gap Delta ≈ 1.7 ± 0.3 meV. This clearly indicates that the gap structure of our investigated systems more likely corresponds to a nodeless s-wave two gaps. [less ▲]

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See detailNeutrophil contribution to spinal cord injury and repair
Neirinckx, Virginie ULg; Coste, Cécile ULg; Franzen, Rachelle ULg et al

in Journal of Neuroinflammation (2014), 11(1),

Spinal cord injuries remain a critical issue in experimental and clinical research nowadays, and it is now well accepted that the immune response and subsequent inflammatory reactions are of significant ... [more ▼]

Spinal cord injuries remain a critical issue in experimental and clinical research nowadays, and it is now well accepted that the immune response and subsequent inflammatory reactions are of significant importance in regulating the damage/repair balance after injury. The role of macrophages in such nervous system lesions now becomes clearer and their contribution in the wound healing process has been largely described in the last few years. Conversely, the contribution of neutrophils has traditionally been considered as detrimental and unfavorable to proper tissue regeneration, even if there are very few studies available on their precise impact in spinal cord lesions. Indeed, recent data show that neutrophils are required for promoting functional recovery after spinal cord trauma. In this review, we gathered recent evidence concerning the role of neutrophils in spinal cord injuries but also in some other neurological diseases, highlighting the need for further understanding the different mechanisms involved in spinal cord injury and repair. [less ▲]

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See detailAntibiotic resistance trend of Staphylococcus aureus isolated between 2010 and 2012 from mastitis cases in Azawak zebu in Niger
Issa Ibrahim, Abdoulkarim ULg; Duprez, Jean-Noël ULg; Bada-Alambedji, Rianatou et al

in African Journal of Microbiology Research (2014), 8((35)), 3271-3275

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See detailIntérêt des cellules stromales mésenchymateuses en transplantation d’organes solides
Delens, Loic ULg; Jouret, François ULg; DETRY, Olivier ULg et al

in Revue Médicale Suisse (2014), 10

Solid organ transplantation (SOT) currently represents the best therapeutic option in end-stage diseases caused by the irrevocable functional loss of an organ. Still, SOT is associated with immunological ... [more ▼]

Solid organ transplantation (SOT) currently represents the best therapeutic option in end-stage diseases caused by the irrevocable functional loss of an organ. Still, SOT is associated with immunological and non-immunological injuries, whose severity impacts on early functional recovery and long-term survival of the transplant. Current research focuses on the identification of innovative approaches to 1) attenuate ischemia/reperfusion-induced damage, 2) accelerate processes of tissue repair, and 3) induce in fine graft tolerance. Encouraging observations from both preclinical studies and clinical trials suggest that the administration of mesenchymal stromal cells at the time of SOT might be beneficial, as a result of theirs immunomodulatory, anti-inflammatory and regenerative properties. [less ▲]

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See detailIntérêt des cellules stromales mésenchymateuses en transplantation d’organes solides
Delens, Loic ULg; Jouret, François ULg; DETRY, Olivier ULg et al

in Revue Médicale Suisse (2014), 10

Solid organ transplantation (SOT) currently represents the best therapeutic option in end-stage diseases caused by the irrevocable functional loss of an organ. Still, SOT is associated with immunological ... [more ▼]

Solid organ transplantation (SOT) currently represents the best therapeutic option in end-stage diseases caused by the irrevocable functional loss of an organ. Still, SOT is associated with immunological and non-immunological injuries, whose severity impacts on early functional recovery and long-term survival of the transplant. Current research focuses on the identification of innovative approaches to 1) attenuate ischemia/reperfusion-induced damage, 2) accelerate processes of tissue repair, and 3) induce in fine graft tolerance. Encouraging observations from both preclinical studies and clinical trials suggest that the administration of mesenchymal stromal cells at the time of SOT might be beneficial, as a result of theirs immunomodulatory, anti-inflammatory and regenerative properties. [less ▲]

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See detailIn vivo skin fluorescence imaging in young Caucasian adults with early malignant melanomas
PIERARD, Gérald ULg; Hermanns-Lê, Trinh ULg; Pierard, Sébastien ULg et al

in Clinical, Cosmetic and Investigational Dermatology (2014), 7

Background: Human cutaneous malignant melanoma (CMM) is an aggressive cancer showing a dramatic worldwide increase in incidence over the past few decades. The most prominent relative epidemiological ... [more ▼]

Background: Human cutaneous malignant melanoma (CMM) is an aggressive cancer showing a dramatic worldwide increase in incidence over the past few decades. The most prominent relative epidemiological increase has been disclosed in young women. The aim of the study was to assess the effects of chronic sun exposures in order to rate the extend of melanocytic stimulations in the vicinity of CMM. Methods: The study was designed to evaluate the melanin distribution and density using ultraviolet light illumination. The present study was performed on surgical excision specimens of thin CMM lesion removed from the upper limbs of 55 Caucasian adults (37 women and 18 men). Two control groups comprised 23 men and 21 women of similar ages who had medium-size congenital melanocytic nevi, also present on the upper limbs. The peritumoral skin was scrutinized using a Visioscan® VC98 device, revealing the faint mosaic melanoderma (FMM) pattern that grossly indicates early signs of chronic photodamage in epidermal melanin units. Results: The median extent of relative FMM was significantly higher in the CMM male group. By contrast, the CMM female group showed a reverse bimodal distribution in FMM size. Only 12/37 (32.5%) of the CMM female group had an increased FMM size, whereas 25/37 (67.5%) of females with CMM had a global FMM extent in the normal range, relative to the controls. Conclusion: Thin CMM supervening in young women appear unrelated to repeat photoexposure. Other mechanisms are possibly involved. [less ▲]

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See detailPathogenicity of Aspergillus clavatus produced in a fungal biofilm bioreactor toward Culex quinquefasciatus (Diptera: Culicidae)
Seye, Fawrou; Bawin, Thomas ULg; Boukraa, Slimane ULg et al

in Journal of Pesticide Science (2014), 39(3), 127-132

Many entomopathogenic fungi have been demonstrated to be potential agents for efficiently controlling mosquito populations. In the present study, we investigated a bioreactor system to produce metabolites ... [more ▼]

Many entomopathogenic fungi have been demonstrated to be potential agents for efficiently controlling mosquito populations. In the present study, we investigated a bioreactor system to produce metabolites and conidia by combining technological advantages of submerged and solid-state fermentations. The efficiency of fungal products was tested toward mosquitoes. Aspergillus clavatus (Eurotiales: Trichocomaceae) was grown by semi-solid-state fermentation in a bioreactor for up to 7 days. Depending on conidial doses (2.5×10^7, 5×10^7, 7.5×10^7, 10×10^7 and 12.5×10^7 conidia/mL), mortality ranged from 37.2±15.0 to 86.3±5.0% toward larvae and from 35.8±2.0 to 85.2±1.5% toward adults. The metabolites (10, 20, 40, 60, 80 and 100% v/v) yielded mortality from 23.7±15.0 to 100.0±0.1% toward larvae, and two sprayed volumes (5 and 10 mL) reached 45.5±1.4 and 75.6±2.6% mortality, respectively, toward adults. [less ▲]

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See detailDevelopmental profiles of GFAP-positive astrocytes in sheep cerebellum
Salouci, Moustafa ULg; Antoine, Nadine ULg; Shikh Al Sook, Mohamad Khir ULg et al

in Veterinary Research Communications (2014)

Astroglial account for the largest glial population in the brain and play a variety of vital functions in the development of the central nervous system (CNS). An immunohistochemical study was performed in ... [more ▼]

Astroglial account for the largest glial population in the brain and play a variety of vital functions in the development of the central nervous system (CNS). An immunohistochemical study was performed in 19 ovine foetuses ranging from two to five months of gestation, one newborn lamb and three adult sheep. Using the anit-glial fibrillary acidic protein (GFAP) marker, several variations were found in the degree of GFAP positive (GFAP+) astrocyte distribution between the different zones in the cerebellum of sheep during brain development. Our study indicates that the first appearance of astrocytes from restricted zones in the cerebellum occurs around the eighth week of gestation. Bergmann cells were found to be present from around the 15th week of gestation onwards. Our findings suggest that the maturation of astrocytes begins in the caudal parts of the cerebellum, developing from their initial ventral regions to spread first to dorsal regions radially within the white matter, then followed by the more rostral parts of the cerebellum. Astrocytes were also found to proliferate in the vermis before appearing in the cerebellar hemispheres. [less ▲]

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See detailNoise Reduction Properties of an Experimental Bituminous Slurry with Crumb Rubber Incorporated by the Dry Process
Bueno, Moises; Luong, Jeanne ULg; Teran, Fernando et al

in Coatings (2014), 4(3), 602-613

Nowadays, cold technology for asphalt pavement in the field of road construction is considered as an alternative solution to conventional procedures from both an economic and environmental point of view ... [more ▼]

Nowadays, cold technology for asphalt pavement in the field of road construction is considered as an alternative solution to conventional procedures from both an economic and environmental point of view. Among these techniques, bituminous slurry surfacing is obtaining an important role due to the properties of the obtained wearing course. The functional performance of this type of surfaces is directly related to its rough texture. Nevertheless, this parameter has a significant influence on the tire/road noise generation. To reduce this undesirable effect on the sound performance, new designs of elastic bituminous slurries have been developed. Within the FENIX project, this work presents the acoustical characterization of an experimental bituminous slurry with crumb rubber from wasted automobile tires incorporated by the dry process. The obtained results show that, under controlled operational parameters, the close proximity sound levels associated to the experimental slurry are considerably lower than those emitted by a conventional slurry wearing course. However, after one year of supporting traffic loads and different weather conditions, the evaluated bituminous slurry, although it conserves the original noise reduction properties in relation to the conventional one, noticeably increases the generated sound emission. Therefore, it is required to continue improving the design of experimental surfaces in order to enhance its long-term performance. [less ▲]

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See detailRole of Imaging in the Staging and Response Assessment of Lymphoma : Consensus of the International Conference on Malignant Lymphomas Imaging Working Group
BARRINGTON, SALLY F.; MIKKHAEEL, N. GEORGE; KOSTAKOGLU, LALE et al

in Journal of Clinical Oncology (2014)

This article comprises the consensus reached to update guidance on the use of PET-CT for staging and response assessment for 18F FDG-avid lymphomas in clinical practice and late-phase trials.

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See detailLabeo rosae (Cypriniformes: Cyprinidae) in the Congo basin: a relict distribution or a historical introduction?
Van SteenBerge, Maarten; Gajdzik, Laura ULg; Chilala, Alex et al

in Journal of Fish Biology (2014)

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See detailA Deep Chandra Observation of the Giant H II Region N11. I. X-Ray Sources in the Field
Nazé, Yaël ULg; Wang, Q. Daniel; Chu, You-Hua et al

in Astrophysical Journal Supplement Series (2014), 213

A very sensitive X-ray investigation of the giant H II region N11 in the Large Megallanic Cloud was performed using the Chandra X-ray Observatory. The 300 ks observation reveals X-ray sources with ... [more ▼]

A very sensitive X-ray investigation of the giant H II region N11 in the Large Megallanic Cloud was performed using the Chandra X-ray Observatory. The 300 ks observation reveals X-ray sources with luminosities down to 10[SUP]32[/SUP] erg s[SUP]–1[/SUP], increasing the number of known point sources in the field by more than a factor of five. Among these detections are 13 massive stars (3 compact groups of massive stars, 9 O stars, and one early B star) with log (L [SUB]X[/SUB]/L [SUB]BOL[/SUB]) ~–6.5 to –7, which may suggest that they are highly magnetic or colliding-wind systems. On the other hand, the stacked signal for regions corresponding to undetected O stars yields log (L [SUB]X[/SUB]/L [SUB]BOL[/SUB]) ~–7.3, i.e., an emission level comparable to similar Galactic stars despite the lower metallicity. Other point sources coincide with 11 foreground stars, 6 late-B/A stars in N11, and many background objects. This observation also uncovers the extent and detailed spatial properties of the soft, diffuse emission regions, but the presence of some hotter plasma in their spectra suggests contamination by the unresolved stellar population. [less ▲]

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See detailStandardization of DiaSorin and Roche automated third generation PTH assays with an international standard: impact on clinical populations
CAVALIER, Etienne ULg; DELANAYE, Pierre ULg; LUKAS, Pierre ULg et al

in Clinical Chemistry & Laboratory Medicine (2014), 52(8), 1137-41

Background: Standardization of parathyroid hormone (PTH) assays is a major issue, especially in hemodialyzed (HD) patients. Two automated third generation PTH assays (Roche Elecsys and DiaSorin Liaison ... [more ▼]

Background: Standardization of parathyroid hormone (PTH) assays is a major issue, especially in hemodialyzed (HD) patients. Two automated third generation PTH assays (Roche Elecsys and DiaSorin Liaison) are now available. These assays are specific for the (1-84) PTH and do not cross-react with the (7-84) fragment, contrary to second generation (intact) assays. We aimed to calibrate the two methods against the WHO International PTH Standard (IS) 95/646 to see if the two assays could provide comparable results in a population of healthy subjects, HD patients and patients suffering from primary hyperparathyroidism (PHP). Methods: We selected 79 healthy subjects and two populations of patients presenting PTH disorders: 56 HD and 27 PHP patients. We reconstituted the IS in a pool of human serum containing undetectable levels of 1-84 PTH and prepared 13 serum standards ranging from 0 to 2000 pg/mL. The standards were run on the two instruments to calibrate the assays on the IS. The different populations were run before and after restandardization. Results: As these kits were differently calibrated, the results obtained after restandarization were significantly different. Restandardization process improved concordance between assays and, taking the analytical variability of the two kits into account, the results could be considered to be similar. Conclusions: Restandardization of automated third generation PTH assays with the WHO 1-84 PTH Standard significantly reduces inter-method variability. Reference ranges and raw values are totally transposable from one method to the other in healthy subjects, but also in diseased patients, e.g., with HD or those suffering from PHP. [less ▲]

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See detailEvaluation of [18F]UCB-H as a novel PET tracer for synaptic vesicle protein 2A in the brain.
Warnock, Geoffrey; Aerts, Joël ULg; Bahri, Mohamed Ali ULg et al

in Journal of Nuclear Medicine (The) (2014), 55(8), 1336-1341

Synaptic vesicle 2 (SV2) proteins are critical to proper nervous system function and are involved in vesicle trafficking. The SV2A isoform has been identified as the binding site of the antiepileptic ... [more ▼]

Synaptic vesicle 2 (SV2) proteins are critical to proper nervous system function and are involved in vesicle trafficking. The SV2A isoform has been identified as the binding site of the antiepileptic levetiracetam (LEV), making it an interesting therapeutic target for epilepsy. [18F]UCB-H is a novel PET imaging agent with a nanomolar affinity for human SV2A. Methods: preclinical PET studies were carried out in isoflurane anesthetized rats. Arterial input function was measured using an arteriovenous shunt and beta microprobe system. [18F]UCB-H was injected IV (140 ± 20 MBq bolus). Results: brain uptake of [18F]UCB-H was high, matching the expected homogeneous distribution of SV2A. The distribution volume (Vt) for [18F]UCB-H was calculated using Logan’s graphical analysis and the effect of LEV pretreatment on Vt measured. In control animals the mean whole-brain Vt was 9.76 ± 0.52 ml/cm3 (mean ± SD, n=4, test-retest), and the mean reproducibility in test-retest studies was 10.4 ± 6.5 %. Uptake of [18F]UCB-H was dose-dependently blocked by pretreatment with LEV (0.1 - 100 mg/kg IV). Conclusion: our results indicate that [18F]UCB-H is a suitable radiotracer for the imaging of SV2A in vivo. This is the first PET tracer for in vivo quantification of SV2A. The necessary steps for implementation of [18F]UCB-H production under GMP conditions and first in human studies are planned. [less ▲]

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See detailcompte rendu de : Geiger, Joseph. Hellenism in the East: studies on Greek intellectuals in Palestine.
Rochette, Bruno ULg

in Bryn Mawr Classical Review [=BMCR] (2014)

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See detailRural Credit and Farms Efficiency : Modelling Farmers Crédit Allocation Decisions, Evidences from Benin
Sossou, Comlan ULg; Noma, Freddy; Yabi, Jacob

in Economics Research International (2014), 2014

This paper analyses farmers’ credit allocation behaviors and their effects on technical efficiency. Data were collected from 476 farmers using themultistage sampling procedure.The stochastic frontier ... [more ▼]

This paper analyses farmers’ credit allocation behaviors and their effects on technical efficiency. Data were collected from 476 farmers using themultistage sampling procedure.The stochastic frontier truncated-normal with conditional mean model is used to assess allocation schemes effects on technical efficiency. Tobitmodel reveals the impact of farmers’ sociodemographic characteristics on efficiency scores. Results reveal that farm revenue (about 2,262,566 Fcfa on average) is positively correlated with land acreage, quantity of labour, and costs of fertilizers and insecticides. Farmers’ behaviors respond to six schemes which are categorized in two allocations contexts : out-farm and in-farm allocations. The model shows that only scheme (e) positively impacts technical efficiency.This scheme refers to the decision to invest credit to purchase better quality of pesticides, herbicides, fertilizers, and so forth. The positive effect of the scheme (c) may be significant under conditions of farmers’ education level improvement. Then, scheme (e) is a better investment for all farmers, but effect of credit allocation to buy agricultural materials is positive only for educated farmers. Efficiency scores are reduced by household size and gender of the household head. Therefore a household with more than 10 members and a woman as head is likely to not be technically efficient. [less ▲]

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See detailReliabilität und Validität des "Voice Handicap Index (VHI) adaptiert an die Gesangsstimme"
Morsomme, Dominique ULg; Barsties, B.; Kropp, J. et al

in Laryngo Rhino Otologie (2014)

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See detailScalar triplet flavored leptogenesis: a systematic approach
Aristizabal Sierra, Diego ULg; Dhen, Mikael; Hambye, Thomas

in Journal of Cosmology and Astroparticle Physics [= JCAP] (2014), 2014

Type-II seesaw is a simple scenario in which Majorana neutrino masses are generated by the exchange of a heavy scalar electroweak triplet. When endowed with additional heavy fields, such as right-handed ... [more ▼]

Type-II seesaw is a simple scenario in which Majorana neutrino masses are generated by the exchange of a heavy scalar electroweak triplet. When endowed with additional heavy fields, such as right-handed neutrinos or extra triplets, it also provides a compelling framework for baryogenesis via leptogenesis. We derive in this context the full network of Boltzmann equations for studying leptogenesis in the flavored regime. To this end we determine the relations which hold among the chemical potentials of the various particle species in the thermal bath. This takes into account the standard model Yukawa interactions of both leptons and quarks as well as sphaleron processes which, depending on the temperature, may be classified as faster or slower than the Universe Hubble expansion. We find that when leptogenesis is enabled by the presence of an extra triplet, lepton flavor effects allow the production of the B-L asymmetry through lepton number conserving CP asymmetries. This scenario becomes dominant as soon as the triplets couple more to leptons than to standard model scalar doublets. In this case, the way the B-L asymmetry is created through flavor effects is novel: instead of invoking the effect of L-violating inverse decays faster than the Hubble rate, it involves the effect of L-violating inverse decays slower than the Hubble rate. We also analyze the more general situation where lepton number violating CP asymmetries are present and actively participate in the generation of the B-L asymmetry, pointing out that as long as L-violating triplet decays are still in thermal equilibrium when the triplet gauge scattering processes decouple, flavor effects can be striking, allowing to avoid all washout suppression effects from seesaw interactions. In this case the amount of B-L asymmetry produced is limited only by a universal gauge suppression effect, which nevertheless goes away for large triplet decay rates. [less ▲]

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See detailExperimental study of wings undergoing active root flapping and pitching
Abdul Razak, Norizham; Dimitriadis, Grigorios ULg

in Journal of Fluids & Structures (2014), 49

This paper presents the results of experiments carried out on mechanical wings undergoing active root flapping and pitching in the wind tunnel. The objective of the work is to investigate the effect of ... [more ▼]

This paper presents the results of experiments carried out on mechanical wings undergoing active root flapping and pitching in the wind tunnel. The objective of the work is to investigate the effect of the pitch angle oscillations and wing profile on the aerodynamic forces generated by the wings. The experiments were repeated for different reduced frequency, airspeed, flapping and pitching kinematics, geometric angle of attack and wing sections (one symmetric and two cambered airfoils). A specially designed mechanical flapper was used, modelled on large migrating birds. It is shown that, under pitch leading conditions, good thrust generation can be obtained at a wide range of Strouhal numbers if the pitch angle oscillation is adjusted accordingly. Consequently, high thrust was measured at both the lowest and the highest tested Strouhal numbers. Furthermore, the work demonstrates that the aerodynamic forces can be sensitive to the Reynolds number, depending on the camber of the wings. Under pitch lagging conditions, where the effective angle of attack amplitude is highest, the symmetric wing was affected by the Reynolds number, generating less thrust at the lowest tested Reynolds value. In contrast, under pure flapping conditions, where the effective angle of attack amplitude was lower but still significant, it was the cambered wings that demonstrated Reynolds sensitivity. [less ▲]

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See detailImpacts d’une association culturale de pois et de pommes de terre sur les pucerons et auxiliaires aphidiphages en Chine
Bosquée, Emilie ULg; Chevalier Mendes Lopes, Thomas ULg; Serteyn, Laurent et al

in Entomologie Faunistique = Faunistic Entomology (2014), 67

Crop associations can have beneficial effects on the control of insect pests. This study was conducted in the Shandong province (China) to assess the impact of potatoes and peas association on the ... [more ▼]

Crop associations can have beneficial effects on the control of insect pests. This study was conducted in the Shandong province (China) to assess the impact of potatoes and peas association on the populations of aphids and their natural enemies. Observations on potato plants and trapping (yellow pan traps) were performed. During the season, the degree of infestation didn’t differ significantly between the association and the pure stand. However, one week after the aphids abundance peak, predators and parasitoid mummies were significantly more abundant in the association compared with the pure stand. Trapping give a more general idea about the richness and diversity of adult natural enemies present in the environment. The parasitoid specie Aphidius gifuensis (Ashmead) was the most abundant among beneficials. Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) were prevalent among predators. Few hoverflies and lacewings were trapped. This study contributes to better evaluate the potential of crop associations as an alternative method to control aphids without insecticides in this region. [less ▲]

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See detailComparison and impact of waste heat recovery technologies on passenger car fuel consumption on a normalized driving cycle
Legros, Arnaud ULg; Guillaume, Ludovic ULg; Diny, Mouad et al

in Energies (2014)

The purpose of this article was to compare different waste heat recovery system technologies designed for automotive applications. A complete literature review is done and results in two comparative ... [more ▼]

The purpose of this article was to compare different waste heat recovery system technologies designed for automotive applications. A complete literature review is done and results in two comparative graphs. In the second part, simulation models are built and calibrated in order to assess the fuel consumption reduction that can be achieved on a real driving cycle. The strength of this article is that the models are calibrated using actual data. Finally, those simulations results are analyzed and the Rankine cycle and turbocompound are the two most profitable solutions. However the simulations of the turbocompound shows its limitations because the impact on the exhaust pressure drop is not taken into account in the assessment of the car fuel consumption. Fuel reduction of up to 6% could be achieved, depending on the driving cycle and the waste heat recovery technology. [less ▲]

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See detailThiophene derivatives with donor–π–acceptor structures for enhanced light-absorption properties and efficient cationic polymerization upon green-light irradiation
Telitel, Sofia; Ouhib, Farid ULg; Fouassier, Jean-Pierre et al

in Macromolecular Chemistry and Physics (2014), 215(16), 1514-1524

Positively charged thermoresponsive nanogels (NGs) are easily synthesized by precipitation polymerization of N-isopropyl­acrylamide and 2-dimethyl(aminoethyl)­methacrylate crosslinked with dendritic ... [more ▼]

Positively charged thermoresponsive nanogels (NGs) are easily synthesized by precipitation polymerization of N-isopropyl­acrylamide and 2-dimethyl(aminoethyl)­methacrylate crosslinked with dendritic polyglycerol. The NGs are characterized in terms of size, thermoresponsive properties, and Z potential. Their potential applications as drug delivery carriers are evaluated by the encapsulation and release of different anticancer drugs. As model drugs, doxorubicin and methotrexate are studied. [less ▲]

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See detailEvaluation of the quantitative performances of Supercritical Fluid Chromatography : from method development to validation
Dispas, Amandine ULg; Lebrun, Pierre ULg; Ziemons, Eric ULg et al

in Journal of Chromatography. A (2014), 1353(Method Validation), 78-88

Recently, the number of papers about SFC increased drastically but scientists did not truly focus their work on quantitative performances of this technique. In order to prove the potential of UHPSFC, the ... [more ▼]

Recently, the number of papers about SFC increased drastically but scientists did not truly focus their work on quantitative performances of this technique. In order to prove the potential of UHPSFC, the present work discussed about the different steps of the analytical life cycle of a method: from development to validation and application. Moreover, the UHPSFC quantitative performances were evaluated in comparison with UHPLC, which is the main technique used for quality control in the pharmaceutical industry and then could be considered as a reference. The methods were developed using Design Space strategy, leading to the optimization of robust method. In this context, when the Design Space optimization shows guarantee of quality, no more robustness study is required prior to the validation. Then, the methods were geometrically transferred in order to reduce the analysis time. The UHPSFC and UHPLC methods were validated based on the total error approach using accuracy profile. Even if UHPLC showed better precision and sensitivity, UHPSFC method is able to give accurate results in a dosing range larger than the 80–120% range required by the European Medicines Agency. Consequently, UHPSFC results are valid and could be used for the control of active substance in a finished pharmaceutical product. Finally, UHPSFC validated method was used to analyse real samples and gave similar results than the reference method (UHPLC). [less ▲]

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See detailIdentification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
Martin, Marjolaine ULg; Biver, Sophie ULg; Steels, Sébastien ULg et al

in Applied and Environmental Microbiology (2014), 80(16), 4958-4967

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two ... [more ▼]

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. [less ▲]

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See detailForage plants as alternative feed resource for sustainable pig production in the tropics: a review
Kambashi Mutiaka, Bienvenu ULg; Boudry, Christelle ULg; Picron, Pascale ULg et al

in Animal (2014), 8(8), 1298-1311

Globally, pressure on concentrate feed resources is increasing, especially in the tropics where many countries are net importers of food. Forage plants are a possible alternative but their use as feed ... [more ▼]

Globally, pressure on concentrate feed resources is increasing, especially in the tropics where many countries are net importers of food. Forage plants are a possible alternative but their use as feed ingredients for pigs raises several issues related to their higher fibre and plant secondary metabolites contents as well as their lower nutritive value. In this paper, the nutritive value of several forage species as well as the parameters that influence this nutritive value in relationship to the plant family, the physiological stage, the plant part and the preservation method (fresh, hay and silage) are reviewed. The influence of the breed and the physiological status of the animal on animal voluntary intake of fibre-rich ingredients, digestibility as related to gastrointestinal volume and transit time and growth performances are also discussed. The final section highlights the assets and drawbacks of forage plants in pig diets and stresses the need for proper economic evaluation to conclude on the benefits of the use of forage plants in pig feed. [less ▲]

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See detailIs wild boar heading towards movement ecology? A review of trends and gaps
Morelle, Kevin ULg; Lehaire, François ULg; Lejeune, Philippe ULg

in Wildlife Biology (2014), 20(4), 196-205

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See detailMulti-period vehicle loading with stochastic release dates
Arda, Yasemin ULg; Crama, Yves ULg; Kronus, David ULg et al

in EURO Journal on Transportation and Logistics (2014), 3(2), 93-119

This paper investigates a multi-period vehicle loading problem with stochastic information regarding the release dates of items to be transported. The deterministic version of the problem can be ... [more ▼]

This paper investigates a multi-period vehicle loading problem with stochastic information regarding the release dates of items to be transported. The deterministic version of the problem can be formulated as a large-scale set covering problem. Several heuristic algorithms are proposed to generate decision policies for the stochastic optimization model over a long rolling horizon. The resulting policies have been extensively tested on instances which display the main characteristics of the industrial case-study that motivated the research. The tests demonstrate the benefits of the multi-period stochastic model over simple myopic strategies. A simple and efficient heuristic is shown to deliver good policies and to be robust against errors in the estimation of the probability distribution of the release dates. [less ▲]

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See detailQuality of Work Life, Burnout, and Stress in Emergency Department Physicians: A Qualitative Review
Bragard, Isabelle ULg; Dupuis, Gilles; Fleet, Richard

in European Journal of Emergency Medicine (2014)

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See detailWASP-117b: a 10-day-period Saturn in an eccentric and misaligned orbit
Lendl, Monika ULg; Triaud, A. H. M. J.; Anderson, D. R. et al

in Astronomy and Astrophysics (2014), 568

We report the discovery of WASP-117b, the first planet with a period beyond 10 days found by the WASP survey. The planet has a mass of M_p = 0.2755 (+/-0.0090) M_jup, a radius of R_p = 1.021 (-0.065 +0 ... [more ▼]

We report the discovery of WASP-117b, the first planet with a period beyond 10 days found by the WASP survey. The planet has a mass of M_p = 0.2755 (+/-0.0090) M_jup, a radius of R_p = 1.021 (-0.065 +0.076) R_jup and is in an eccentric (e = 0.302 +/-0.023), 10.02165 +/- 0.00055 d orbit around a main-sequence F9 star. The host star's brightness (V=10.15 mag) makes WASP-117 a good target for follow-up observations, and with a planetary equilibrium temperature of T_eq = 1024 (-26 +30) K and a low planetary density (rho_p = 0.259 (-0.048 +0.054) rho_jup) it is one of the best targets for transmission spectroscopy among planets with periods around 10 days. From a measurement of the Rossiter-McLaughlin effect, we infer a projected angle between the planetary orbit and stellar spin axes of beta = -44 (+/-11) deg, and we further derive an orbital obliquity of psi = 69.5 (+3.6 -3.1) deg. Owing to the large orbital separation, tidal forces causing orbital circularization and realignment of the planetary orbit with the stellar plane are weak, having had little impact on the planetary orbit over the system lifetime. WASP-117b joins a small sample of transiting giant planets with well characterized orbits at periods above ~8 days. [less ▲]

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See detailThe cerato-mandibular ligament: a key functional trait for grazing in damselfishes (Pomacentridae)
Olivier, Damien ULg; Frederich, Bruno ULg; Spanopoulos-Zarco, Milton et al

in Frontiers in Zoology (2014), 11(63), 1-14

Introduction: The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The ... [more ▼]

Introduction: The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The Pomacentridae possess a synapomorphic trait, the cerato-mandibular (c-md) ligament, which joins the hyoid bar to the inner part of the lower jaw. It has previously been shown that this ligament is a key trait in communication in damselfishes because it enables them to slam the oral jaws shut causing teeth collision and sound production. This specific behavior of mouth closing could, however, also be used for other tasks, such as feeding. Many territorial damselfishes are referred to as farmers, due to their ability to manage algal crops on which they feed. This study hypothesizes that the c-md ligament provides an advantage for grazing filamentous algae, and should thus be considered a key trait for farming behavior. Results: The kinematic patterns associated with sound production and biting filamentous algae or attached animal prey are all based on the same mechanism and are associated with a slam of the oral jaws. We observed that transection of the c-md ligaments makes the fish unable to perform such actions. We also counted biting rates on filamentous algae in fish with and without the c-md ligament and observed a drop of more than 80% in the latter. Conclusion: This study shows that the c-md ligament is a key trait both for sound production and for grazing activities in damselfishes. The buccal jaw slam enables the fish to perform accurate strikes on small filamentous algae. This kind of bite probably plays a major role in farming activity and allows grazing damselfishes to occupy distinct niches, possibly increasing their competitive evolutionary success. [less ▲]

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See detailA Remark on the Poroelastic Center of Dilation
Huynen, Alexandre ULg; Detournay, Emmanuel

in Journal of Elasticity (2014), 116(2), 189-206

In the original derivation due to Dougall (1897), the center of dilation is constructed from the superposition of orthogonal force dipoles. However, it can also be built by combining dislocation dipoles ... [more ▼]

In the original derivation due to Dougall (1897), the center of dilation is constructed from the superposition of orthogonal force dipoles. However, it can also be built by combining dislocation dipoles. While the two representations are equivalent in elasticity, the different nature of the force and dislocation dipoles leads to different transient behaviors in poroelasticity, when the response evolves from undrained to drained. Indeed, we show that the poroelastic solution of a center of dilation is obtained by superposing the corresponding elastic solution and either an instantaneous fluid sink or an instantaneous fluid source, depending on whether the center of dilation is treated as a singular stress or displacement discontinuity. A simple explanation to the instantaneous injection or withdrawal of a finite volume of fluid from/to the singularity is given by adopting an exploded view of the poroelastic center of dilation. The exploration of the drained, undrained and transient responses of this singularity also requires to clarify the close relationship between an instantaneous fluid source and a continuous fluid dodecapole, an object constructed by collapsing onto a point three orthogonal pairs of fluid dipoles. [less ▲]

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See detailCase studies of incorporation of prior information in electrical resistivity tomography: comparison of different approaches
Caterina, David ULg; Hermans, Thomas ULg; Nguyen, Frédéric ULg

in Near Surface Geophysics (2014), 12(4), 451-465

Many geophysical inverse problems are ill-posed and their solution non-unique. It is thus important to reduce the amount of mathematical solutions to more geologically plausible models by regularizing the ... [more ▼]

Many geophysical inverse problems are ill-posed and their solution non-unique. It is thus important to reduce the amount of mathematical solutions to more geologically plausible models by regularizing the inverse problem and incorporating all available prior information in the inversion process. We compare three different ways to incorporate prior information for electrical resistivity tomography (ERT): using a simple reference model or adding structural constraints to Occam's inversion and using geostatistical constraints. We made the comparison on four real cases representing different field applications in terms of scales of investigation and level of heterogeneities. In those cases, when electromagnetic logging data are available in boreholes to control the solution, it appears that incorporating prior information clearly improves the correspondence with logging data compared to the standard smoothness constrain. However, the way to incorporate it may have a major impact on the solution. A reference model can often be used to constrain the inversion; however, it can lead to misinterpretation if its weight is too strong or the resistivity values inappropriate. When the computation of the vertical and/or horizontal correlation length is possible, the geostatistical inversion gives reliable results everywhere in the section. However, adding geostatistical constraints can be difficult when there is not enough data to compute correlation lengths. When a known limit between two layers exists, the use of structural constrain seems to be more indicated particularly when the limit is located in zones of low sensitivity for ERT. This work should help interpreters to include their prior information directly into the inversion process through an appropriate way. [less ▲]

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See detailPhénologie et intensité de la migration postnuptiale diurne en Ardenne du nord-est par le suivi migratoire (Ramecroix, 2006 à 2010)
Beckers, Arnaud ULg; Baron, André

in Aves (2014), 51(2), 87-106

We present the results of five years of monitoring postnuptial migration in the northeast Ardennes (the Ramecroix site) from 2006 to 2010. The timing and intensity of migration have been calculated for ... [more ▼]

We present the results of five years of monitoring postnuptial migration in the northeast Ardennes (the Ramecroix site) from 2006 to 2010. The timing and intensity of migration have been calculated for the 19 commonest species. Phenology for each species varied markedly from year to year. The passage of two particular species (Northern Lapwing and Song Trush) was extremely concentrated in time. Comparison with data from the Netherland and from Les Awirs (Liège, Belgium) show differences between locations, for which we propose some explanations. The full dataset constitute a useful benchmark for future studies on the spatial variability of migration flow. [less ▲]

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See detailDetection of nitric oxide by diaminofluorescein visualizes the skeleton in living zebrafish.
Renn, Jörg ULg; Pruvot, Benoist; Muller, Marc ULg

in Journal of Applied Ichthyology (2014), 30(4), 701706

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone ... [more ▼]

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone research in fish, but do not visualize cartilage. In the present study, we show that nitric oxide (NO) labelling by DAF-FM DA visualizes both bone and cartilage in vivo during zebrafish skeletogenesis. NO detection performed in Tg(osterix:mCherry) or in combination with Alizarin Red in wild-type zebrafish reveals that intense staining through NO labelling colocalizes with the appearance of osteoblasts and characterizes ossified structures. Cartilage structures are clearly distinguished in the living larvae, although the labelling is less intensive when compared to ossified structures. This method is the first and easy to handle alternative to cartilage and bone double stainings on fixed samples. In contrast to most live skeletal stainings, which only stain the mineralized bone structures, this protocol in addition allows in vivo visualization of cartilage. [less ▲]

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See detailThe replication characteristics of infectious laryngotracheitis virus (ILTV) in the respiratory and conjunctival mucosa
Reddy, V.R.; Steukers, L.; Li, Y. et al

in Avian Pathology : Journal of the W.V.P.A (2014), 21

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See detailOrganic and mineral imprints in fossil photosynthetic mats of an East Antarctic lake
Lepot, Kevin; Compère, Philippe ULg; Gerard, E et al

in Geobiology (2014), 12(5), 424-450

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See detailHow to correctly determine the kinetics of a photocatalytic degradation reaction?
Pirard, Sophie ULg; Malengreaux, Charline ULg; Toye, Dominique ULg et al

in Chemical Engineering Journal (2014), 249

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See detailNatural intrauterine infection with Schmallenberg virus in malformed newborn calves: pathology and distribution of viral RNA
Bayrou, Calixte ULg; Garigliany, Mutien-Marie ULg; Sarlet, Michaël ULg et al

in Emerging Infectious Diseases (2014), 20(8),

We comprehensively surveyed morphologic alterations in calves naturally infected in utero by Schmallenberg virus (SBV) and born deformed. SBV-specific RNA was distributed unevenly in different tissues ... [more ▼]

We comprehensively surveyed morphologic alterations in calves naturally infected in utero by Schmallenberg virus (SBV) and born deformed. SBV-specific RNA was distributed unevenly in different tissues. Implications for diagnosic procedures are highlighted. [less ▲]

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See detailPhytosanitary treatment of European pallets by microwave: developing a program to ensure compliance with ISPM 15 and monitoring its efficacy on the house longhorn beetle (Hylotrupes bajulus L.)
Henin, Jean-Marc; Leyman, Michael; Bauduin, Aline et al

in European Journal of Wood and Wood Products (2014)

Microwave heating was recently approved by the FAO as a significantly effective phytosanitary treatment for wood packaging material. According to ISPM 15 (FAO 2009), the target organisms are eradicated if ... [more ▼]

Microwave heating was recently approved by the FAO as a significantly effective phytosanitary treatment for wood packaging material. According to ISPM 15 (FAO 2009), the target organisms are eradicated if a temperature higher than 60 °C is maintained for 60 s across the entire profile of the board (i.e. 60 °C/60 s). A study using pallet boards was carried out in order to set up a treatment program that would meet ISPM 15 requirements in terms of wood temperature and insect mortality. A 4 m-long industrial tunnel oven (maximum power of 28.8 kW) was used to carry out the experiments. Temperature was measured by means of a VarioCAM® infrared camera. The most relevant results we found were: (i) achieving a temperature of 63.2 °C (Populus sp.) or 64.8 °C (Pinus sylvestris L.) on the upper surface of 22 mm-thick boards enabled compliance with FAO requirements (i.e. 60 °C/60 s), whatever the moisture content, basic density and initial temperature of the wood (provided the latter exceeded 0 °C); (ii) larvae >150 mg represented the most microwave-resistant life stage of Hylotrupes bajulus L.; (iii) the mortality rate of the larvae was influenced by the moisture content of the boards. Using the Gompertz model, we estimated the upper surface temperature that would be needed to achieve a 99.99683% mortality rate (the Probit 9 mortality level of efficacy) for the most microwave-resistant life stage of H. bajulus. This temperature was estimated to be 46.8 °C and 57 °C for wood with >50 and <25% moisture content, respectively. [less ▲]

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See detailNew carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

in Journal of Applied Microbiology (2014)

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing ... [more ▼]

Aims Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. Methods and Results Both libraries were screened on esculin-supplemented medium so as to isolate candidates showing β-glucosidase activity. Candidate analysis revealed seven putative β-glycosidases and two putative glycosyltransferases, displaying 25 to 82% identity to known enzymes. The putative β-glycosidases belong to families GH1, GH3 and GH20 and the two putative glycosyltransferases, probably, to new families. In characterization tests performed on bacteria in suspension or spread on agar plates, some candidates appeared to hydrolyse several natural and synthetic substrates. These tests also highlighted interesting industrial characteristics, such as the activity of four β-glycosidases under alkaline conditions and the esculin-hydrolysing activity of a β-glucosidase candidate in the presence of glucose. Conclusions Seven putative β-glycosidases and two putative glycosyltransferases were found by functional screening of two metagenomic DNA libraries derived from agricultural soil. Significance and Impact of the Study This study has identified β-glycosidases and putative glycosyltransferases that have or may have interesting industrial characteristics. [less ▲]

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See detailNeoadjuvant chemotherapy in breast cancer induces miR-34a and miR-122 expression
FRERES, Pierre ULg; JOSSE, Claire ULg; Bovy, Nicolas ULg et al

in Journal of Cellular Physiology (2014)

Circulating microRNAs (miRNAs) have been extensively studied in cancer as biomarkers but little is known regarding the influence of anti-cancer drugs on their expression levels. In this article, we ... [more ▼]

Circulating microRNAs (miRNAs) have been extensively studied in cancer as biomarkers but little is known regarding the influence of anti-cancer drugs on their expression levels. In this article, we describe the modifications of circulating miRNAs profile after neoadjuvant chemotherapy (NAC) for breast cancer. The expression of 188 circulating miRNAs was assessed in the plasma of 25 patients before and after NAC by RT-qPCR. Two miRNAs, miR- 34a and miR-122, that were significantly increased after NAC, were measured in tumor tissue before and after chemotherapy in 7 patients with pathological partial response (pPR) to NAC. These 2 chemotherapy-induced miRNAs were further studied in the plasma of 22 patients with adjuvant chemotherapy (AC) as well as in 12 patients who did not receive any chemotherapy. Twenty-five plasma miRNAs were modified by NAC. Among these miRNAs, miR-34a and miR-122 were highly upregulated, notably in pPR patients with aggressive breast cancer. Furthermore, miR-34a level was elevated in the remaining tumor tissue after NAC treatment. Studying the kinetics of circulating miR-34a and miR-122 expression during NAC revealed that their levels were especially increased after anthracycline-based chemotherapy. Comparisons of the plasma miRNA profiles after NAC and AC suggested that chemotherapy-induced miRNAs originated from both tumoral and non-tumoral compartments. This study is the first to demonstrate that NAC specifically induces miRNA expression in plasma and tumor tissue, which might be involved in the anti-tumor effects of chemotherapy in breast cancer patients. [less ▲]

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See detailSound production to electric discharge: sonic muscle evolution in progress in Synodontis spp. catfishes (Mochokidae)
Boyle, Kelly; Colleye, Orphal ULg; Parmentier, Eric ULg

in Proceedings of the Royal Society B : Biological Sciences (2014), 281

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See detailPhotographier le travail, représenter le futur. Iconographie du chantier des Halles à Paris
Dondero, Maria Giulia ULg

in Communication et Langages (2014), 180

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from ... [more ▼]

This article investigates the semiotic strategies of visual representation in the urban space, as exemplified by the renovation project of the Forum des Halles in Paris. The corpus of images, taken from the brochure of the construction site and from the website www.parisleshalles.fr, includes photographic documentation and illustrations of the future buildings. The analysis of the visual enunciation marks out the relative simulacra of the producers and consumers of these images, and a semiotic study of the modes of representation of the buildings (virtualisation, actualisation, realisation, etc.) helps understanding how the present and the future are articulated in the iconography of a construction work in progress. [less ▲]

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See detailSeasonal variability of meiofauna, especially harpacticoid copepods, in Posidonia oceanica macrophytodetritus accumulations
Mascart, Thibaud ULg; Lepoint, Gilles ULg; Deschoemaeker, Silke et al

in Journal of Sea Research (2014)

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated ... [more ▼]

The overall aim of this study was (1) to assess the diversity and density of meiofauna taxa, especially harpacticoid copepod species, present within accumulated seagrass macrophytodetritus on unvegetated sand patches and (2) to elucidate the community structure of detritus-associated harpacticoid copepods in relation to natural temporal variability of physico-chemical characteristics of accumulations. This was investigated in a Posidonia oceanica (L.) Delile seagrass ecosystem in the northwest Mediterranean Sea (Bay of Calvi, Corsica, 42°35’N, 8°43’E) using a triplicate macrophytodetritus core field sampling in two contrasting sites over the four seasons of 2011. Meiofauna higher taxa consisted of 50% Copepoda, which 87% belonged to the Harpacticoida order. Nematoda was the second most abundant taxa. The copepod community displayed a wide variety of morphologically similar and ecologically different species (i.e. mesopsammic, phytal, phytal-swimmers, planktonic and parasitic). The harpacticoid copepod community followed a strong seasonal pattern with highest abundances and species diversity in May-August, revealing a link with the leaf litter epiphyte primary production cycle. Aside from the important role in sheltering, housing and feeding potential of macrophytodetritus, a harpacticoid community BEST analysis demonstrated a positive correlation with habitat complexity and a negative correlation with water movements and P. oceanica leaf litter accumulation. [less ▲]

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See detailThe vegetative state/unresponsive wakefulness syndrome: a systematic review of prevalence studies
Van Erp, WS; Lavrijsen, JC; Van De Laar, FA et al

in European Journal of Neurology (2014)

One of the worst outcomes of acquired brain injury is the vegetative state, recently renamed ‘unresponsive wakefulness syndrome’ (VS/UWS). A patient in VS/UWS shows reflexive behaviour such as spontaneous ... [more ▼]

One of the worst outcomes of acquired brain injury is the vegetative state, recently renamed ‘unresponsive wakefulness syndrome’ (VS/UWS). A patient in VS/UWS shows reflexive behaviour such as spontaneous eye opening and breathing, but no signs of awareness of the self or the environment. We performed a systematic review of VS/UWS prevalence studies and assessed their reliability. Medline, Embase, the Cochrane Library, CINAHL and PsycINFO were searched in April 2013 for cross-sectional point or period prevalence studies explicitly stating the prevalence of VS/UWS due to acute causes within the general population. We additionally checked bibliographies and consulted experts in the field to obtain ‘grey data’ like government reports. Relevant publications underwent quality assessment and data-extraction. We retrieved 1032 papers out of which 14 met the inclusion criteria. Prevalence figures varied from 0.2 to 6.1 VS/UWS patients per 100 000 members of the population. However, the publications’ methodological quality differed substantially, in particular with regards to inclusion criteria and diagnosis verification. The reliability of VS/UWS prevalence figures is poor. Methodological flaws in available prevalence studies, the fact that 5/14 of the studies predate the identification of the minimally conscious state (MCS) as a distinct entity in 2002, and insufficient verification of included cases may lead to both overestimation and underestimation of the actual number of patients in VS/UWS. [less ▲]

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See detailLiver proteomic response to hypertriglyceridemia in human-apolipoprotein C-III transgenic mice at cellular and mitochondrial compartment levels
Ehx, Grégory ULg; Gerin, Stéphanie ULg; Mathy, Grégory et al

in Lipids in Health and Disease (2014), 13

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute ... [more ▼]

Background: Hypertriglyceridemia (HTG) is defined as a triglyceride (TG) plasma level exceeding 150 mg/dl and is tightly associated with atherosclerosis, metabolic syndrome, obesity, diabetes and acute pancreatitis. The present study was undertaken to investigate the impact of hypertriglyceridemia on the mitochondrial, sub-mitochondrial and cellular proteomes in the hepatocytes of a hypertriglyceridemic transgenic mouse model overexpressing the human apolipoproteinC-III. Methods: Quantitative comparative proteomics (2D-DIGE) was carried out in both “low-expressor” (LE) and “high-expressor” (HE) mice, respectively exhibiting moderate and severe HTG, to characterize the effect of the TG plasma level on the proteomic response. Results: The mitoproteome analysis revealed the occurrence of a large-scale adaptation in transgenic mice consisting of a general down-regulation of matricial proteins and up-regulation of inner membrane proteins. Remarkably, the magnitude of these proteomic changes appears to strongly depend on the TG plasma level. Altogether, our different analyses indicate that, in HE mice, the capacity of several metabolic pathways is altered to promote the availability of acetyl-CoA, glycerol-3-phosphate, ATP and NADPH for de novo TG biosynthesis. The up-regulation of several cytosolic ROS detoxifying enzymes also tend to confirm that the cytoplasm of HTG mice is subjected to oxidative stress as previously stated. The up-regulation of cytosolic ferritin indicates that iron over-accumulation could take place in the cytosol of HE mice hepatocytes and contribute to (i) enhance oxidative stress and (ii) promote cellular proliferation. Conclusions: The present analyses demonstrate that important TG dose-responsive metabolic adaptations are set up in human apolipoproteinC-III-overexpressing mice. Our results indicate that these adaptations could support the higher TG production rates which have been previously reported in this HTG model, and also suggest that cytosolic oxidative stress may result from FFA over-accumulation, iron overload and enhanced activity of some ROS-producing catabolic enzymes. [less ▲]

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See detailDetermination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).
Lecrenier, Marie-Caroline ULg; Ledoux, Quentin; Berben, Gilbert et al

in Scientific reports (2014), 4

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone ... [more ▼]

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application. [less ▲]

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See detailAssessing spatiotemporal variability and trends (2000-2013) of modeled and measured Greenland Ice Sheet albedo
Alexander, P.; Tedesco, M.; Fettweis, Xavier ULg et al

in Cryosphere Discussions (The) (2014), 8

Accurate measurements and simulations of Greenland Ice Sheet (GrIS) surface albedo are essential, given the crucial role of surface albedo in modulating the amount of absorbed solar radiation and ... [more ▼]

Accurate measurements and simulations of Greenland Ice Sheet (GrIS) surface albedo are essential, given the crucial role of surface albedo in modulating the amount of absorbed solar radiation and meltwater production. In this study, we assess the spatio-temporal variability of GrIS albedo (during June, July, and August) for the period 2000–2013. We use two remote sensing products derived from data collected by the Moderate Resolution Imaging Spectroradiometer (MODIS), as well as outputs from the Modèle Atmosphérique Régionale (MAR) regional climate model (RCM) and data from in situ automatic weather stations. Our results point to an overall consistency in spatiotemporal variability between remote sensing and RCM albedo, but reveal a difference in mean albedo of up to ~0.08 between the two remote sensing products north of 70° N. At low elevations, albedo values simulated by the RCM are positively biased with respect to remote sensing products and in situ measurements by up to ~0.1 and exhibit low variability compared with observations. We infer that these differences are the result of a positive bias in simulated bare-ice albedo. MODIS albedo, RCM outputs and in situ observations consistently point to a~decrease in albedo of −0.03 to −0.06 per decade over the period 2003–2013 for the GrIS ablation zone (where there is a net loss of mass at the GrIS surface). Nevertheless, satellite products show a~decline in albedo of −0.03 to −0.04 per decade for regions within the accumulation zone (where there is a net gain of mass at the surface) that is not confirmed by either the model or in situ observations. [less ▲]

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