References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailPsychiatrie en osteoporose
Halkin, V; Reginster, Jean-Yves ULg

in Agenda Psychiatrie (L') (2000), 14

Detailed reference viewed: 9 (1 ULg)
Full Text
See detailMotivations et apports d'une prise en charge ambulatoire pluridisciplinaire de la santé des adolescents
Bourguignon, Jean-Pierre ULg; Grignard, Sophie ULg; Glowacz, Fabienne ULg et al

in Bulletin d'Education du Patient à sa Maladie (2000), 19(3), 126-129

D’importants problèmes de santé sont identifiés par les professionnels chez les adolescents. Toutefois, ces problèmes de santé ne sont que peu ou pas soulevés par les adolescents eux-mêmes car ils ... [more ▼]

D’importants problèmes de santé sont identifiés par les professionnels chez les adolescents. Toutefois, ces problèmes de santé ne sont que peu ou pas soulevés par les adolescents eux-mêmes car ils consultent peu à ce sujet. En particulier, ils évoquent rarement les aspects psychosociaux des problèmes auxquels ils sont confrontés. Ils fonctionnent dans le clivage entre somatique et psychologique bien que ces deux aspects soient indissociables. Les adolescents sont souvent amenés en consultation par d’autres, dont les parents. Un travail d’identification de la demande réelle de l’adolescent est donc nécessaire pour que celui-ci puisse se mobiliser vers une prise en charge. La confidentialité est cruciale de même que le respect de l’adolescent, ainsi que la reconnaissance de ses craintes et des limites qu’il met à l’accès aux informations par l’anamnèse et l’examen clinique. Il appartient au médecin de lire la demande de l’adolescent dans un double registre, à la fois somatique et psychique et de resituer cette demande dans le contexte de vie particulier à l’adolescent. Aussi, la lecture de la demande intégrera non seulement les problèmes invoqués par l’adolescent mais aussi ceux relevés par l’anamnèse et l’examen clinique. Une telle lecture peut prendre du temps pour les intervenants de santé mais aussi pour l’adolescent, avant qu’il n’aboutisse à l’émergence de sa demande et, dès lors, à une demande de prise en charge. Celle-ci devra parfois faire appel à une équipe spécifique et pluridisciplinaire telle que celle où collaborent les auteurs du présent article, au Centre de Santé de l’Adolescent à Liège. [less ▲]

Detailed reference viewed: 326 (35 ULg)
Full Text
Peer Reviewed
See detailFormes et formalités. Remarques sur la poétique du nom chez Mallarmé
Durand, Pascal ULg

in Formules (2000), 4

Detailed reference viewed: 48 (13 ULg)
Full Text
Peer Reviewed
See detailInduction of phenolic compounds in two cultivars of cucumber by treatment of healthy and powdery mildew-infected plants with extracts of Reynoutria sachalinensis
Daayf, Fouad; Ongena, MARC ULg; Boulanger, Renald et al

in Journal of Chemical Ecology (2000), 26

Accumulation of phenolic compounds (p-coumaric, caffeic, and ferulic acids and p-coumaric acid methyl ester) was followed in susceptible (Mustang) and tolerant (Flamingo) cucumber (Cucumis sativus ... [more ▼]

Accumulation of phenolic compounds (p-coumaric, caffeic, and ferulic acids and p-coumaric acid methyl ester) was followed in susceptible (Mustang) and tolerant (Flamingo) cucumber (Cucumis sativus) cultivars. The objective was to determine whether these compounds played a role in resistance against powdery mildew following a prophylactic treatment with Milsana (leaf extracts from the giant knot weed Reynoutria sachalinensis, polygonaceae). This treatment significantly reduced the incidence of powdery mildew in both cultivars. Phenolic compounds were extracted from leaves. In the hydrolyzed fraction containing phenolic aglycones, levels of p-coumaric, caffeic, and ferulic acids and of p-coumaric acid methyl ester increased in all treatments (with leaf extracts of R. Sachalinensis, powdery mildew, or both) except the control, one or two days after treatment. In the fraction containing free phenolics, from the tested compounds, only ferulic acid showed an increase in cv. Flamingo (tolerant), and was particularly evident following treatments. On the other hand, the amounts of hydroxycinnamic acids increased rapidly in the two cultivars following Milsana treatment, suggesting their role in disease reduction. All compounds showed antifungal activity when tetsed against common pathogens of cucumber (Botrytis cinerea, Pythium ultimum, and P. aphanidermatum), but in general methyl esters were more fungitoxic than their corresponding free acids. This study suggests that cucumber is able to release antifungal compounds that are instrumental in repressing powdery mildew infection. This response is seemingly independent from the level of genetic resistance associated with each cultivar. [less ▲]

Detailed reference viewed: 67 (0 ULg)
Full Text
See detailInitial Medical Management of Severe Acute Ulcerative Colitis
Belaiche, Jacques ULg; Louis, Edouard ULg

in Acta Gastro-Enterologica Belgica (2000), 63(3, Jul-Sep), 275-8

Severe colitis is life-threatening complication of ulcerative colitis. Early recognition of the severity of the colitis, intensive medical therapy and prompt surgery when necessary have all contributed to ... [more ▼]

Severe colitis is life-threatening complication of ulcerative colitis. Early recognition of the severity of the colitis, intensive medical therapy and prompt surgery when necessary have all contributed to improved outcome. Initial medical treatment should be instituted as soon as the diagnosis is made with an intravenous corticosteroid associated with supportive treatment. If the patient fails to response to this intensive treatment after 5-7 days, cyclosporin should be initiated. If cyclosporin is not used then colectomy should be performed immediately. Moreover, significant deterioration at any point during medical therapy is an indication for colectomy. The gravity of the patient's condition require close interaction between physician an surgeon. [less ▲]

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailHigh Resolution Electron Energy Loss Spectroscopy Investigation of the Vibrational Structure and Order of an Octadecanethiol Monolayer on Gold Substrates
Duwez, Anne-Sophie ULg; Yu, Li-Ming; Riga, Joseph et al

in Journal of Physical Chemistry B (2000), 104

The vibrational spectra induced by electron beam from octadecanethiol monolayers adsorbed on gold are reported as a contribution to study in detail the structure of the layers, through an analysis of the ... [more ▼]

The vibrational spectra induced by electron beam from octadecanethiol monolayers adsorbed on gold are reported as a contribution to study in detail the structure of the layers, through an analysis of the interaction mechanisms between the electrons and these organic ultrathin films. To show the different spatial distribution of scattered electrons from dipole and impact interactions with the monolayer, angular distributions of elastic and inelastic scattered electrons were measured for various primary energies. The absolute electron scattering loss probabilities from both dipole and impact interactions were estimated for different vibrational modes. The study of these different mechanisms was first hampered by the poor order of the layers adsorbed on evaporated gold. It became therefore highly desirable to perform the experiments on a single-crystal substrate. Using Au(111) and Au(100) single crystals as substrates, it was possible to separate the spectral response from impact and dipole interacting electrons. A negative ion resonant behavior has been observed in the energy dependence of the electron scattering differential cross sections [less ▲]

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailL'antibiothérapie en néonatalogie: mise-au-point
Langhendries, JP; Kalenga, Masendu ULg; Battisti, Oreste ULg et al

in Journal du Pédiatre Belge (2000), 2

Detailed reference viewed: 82 (11 ULg)
See detailLa mémoire de travail : une approche neuropsychologique et par imagerie cérébrale
Collette, Fabienne ULg; Van der Linden, Martial ULg

in Thérapie (2000), 55(4), 455-460

The working memory model proposed by Baddeley and Hitch has frequently been used in cognitive psychology and neuropsychological studies. This model consists of several interacting components, responsible ... [more ▼]

The working memory model proposed by Baddeley and Hitch has frequently been used in cognitive psychology and neuropsychological studies. This model consists of several interacting components, responsible for the storage and processing of the information stored in working memory. Many neuropsychological and functional imagery data are consistent with that formulation of working memory. Moreover, many cognitive tasks have been specifically designed to explore particular aspects of working memory functioning. Taken as a whole, these data confirm that the working memory model remains a useful theoretical framework to explore memory functioning both in normal subjects and in pathological conditions. [less ▲]

Detailed reference viewed: 22 (6 ULg)
Full Text
Peer Reviewed
See detailRecension: "Théorie critique du principe général de bonne foi en droit privé"
Kohl, Benoît ULg

in Actualités du Droit (2000)

Detailed reference viewed: 213 (5 ULg)
Peer Reviewed
See detailLa pleuropneumonie contagieuse bovine
Linden, Annick ULg; Desmecht, Daniel ULg

in Vlaams Diergeneeskundig Tijdschrift (2000), 69

Detailed reference viewed: 17 (2 ULg)
Full Text
Peer Reviewed
See detailSomatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and their metabolic pathways
Kevers, Claire ULg; Le Gal, Nathalie; Monteiro, Marta et al

in Plant Growth Regulation (2000), 31(3), 209-214

A callus with embryogenic capacity was generated from root sections of Panax ginseng and used as an inoculum source for embryogenic liquid cultures in a three-step process: - a suspension culture of cell ... [more ▼]

A callus with embryogenic capacity was generated from root sections of Panax ginseng and used as an inoculum source for embryogenic liquid cultures in a three-step process: - a suspension culture of cell aggregates in the presence of an auxin/cytokinin mixture, - an induction medium containing auxin only (for 5 to 30 days), - a regeneration medium containing cytokinin only (for one month). Up to 25 embryos were recovered per 2.5 g of aggregates in these conditions. Incorporation of polyamines or their precursors arginine and ornithine into either the induction or regeneration media increased the number of embryos produced by up to 4 times. Inhibitors of both biosynthesis and biodegradation of polyamines reduced the number of embryos. These results support earlier findings of the role of polyamines in the process of somatic embryogenesis. The success of these liquid cultures opens up the possibility of producing somatic embryos of Panax ginseng in bioreactors. [less ▲]

Detailed reference viewed: 68 (1 ULg)
Peer Reviewed
See detailPerturbed Proximal Point Algorithm with Nonquadratic Kernel
Brohé, Myrana; Tossings, Patricia ULg

in Serdica Mathematical Journal (2000), 26

Let H be a real Hilbert space and T be a maximal monotone operator on H. A well-known algorithm, developed by R.T. Rockafellar, for solving the problem "To find x in H such that 0 in Tx" is the proximal ... [more ▼]

Let H be a real Hilbert space and T be a maximal monotone operator on H. A well-known algorithm, developed by R.T. Rockafellar, for solving the problem "To find x in H such that 0 in Tx" is the proximal point algorithm. Several genaralizations have been considered by several authors: introduction of a perturbation, introduction of a variable metric in the perturbed algorithm, introduction of a pseudo-metric in place of the classical regularization, ... We summarize some of these extensions by taking into account a pseudo-metric as regularization and a perturbation in an inexact version of the algorithm. [less ▲]

Detailed reference viewed: 25 (1 ULg)
Peer Reviewed
See detailInternal and International migration. Chinese Perspectives.
Florence, Eric ULg

in Revue Bibliographique de Sinologie = Review of Bibliography in Sinology (2000), Vol. XVII

Detailed reference viewed: 45 (0 ULg)
Full Text
Peer Reviewed
See detailInfluence of colors on habituation of visual evoked potentials in patients with migraine with aura and in healthy volunteers.
Afra, J; Ambrosini, A; Génicot, Roger ULg et al

in Headache (2000), 40(1), 36-40

OBJECTIVE: To investigate whether colored glasses influence the habituation of visual evoked potentials. BACKGROUND: We have previously shown that during pattern-reversal stimulations lasting 2 minutes ... [more ▼]

OBJECTIVE: To investigate whether colored glasses influence the habituation of visual evoked potentials. BACKGROUND: We have previously shown that during pattern-reversal stimulations lasting 2 minutes the amplitude of the visual evoked potential increases in migraine with and without aura between attacks, whereas it decreases in healthy volunteers. Red light was found to increase visually evoked EEG fast activity only in children with migraine with aura. Wearing rose-tinted glasses for 4 months decreased attack frequency in parallel with a reduction of the visually evoked EEG fast activity. METHODS: We compared the change in amplitude of the visual evoked potential using five different tinted glasses in 12 patients with migraine with aura and in 10 healthy volunteers. During continuous stimulation at 3.1 Hz, five blocks of 50 responses were sequentially averaged using red, yellow, green, blue, and grey glasses and without glasses in a random order and analyzed in terms of latencies and N1-P1 amplitudes. Amplitude changes were calculated for each block by comparison with the first block in every condition and analyzed statistically using Zerbe's method. RESULTS: In healthy volunteers, the visual evoked potential amplitude increased with red glasses compared to without glasses (P = .05) or with green glasses (P = .03). In patients with migraine with aura, no significant difference was detected using colored glasses. Our findings in healthy volunteers are in line with earlier reports of increased excitability of the human visual cortex when exposed to red light. The lack of such a pattern in patients with migraine with aura suggests that the visual cortex is interictally hypoexcitable rather than hyperexcitable, which is consistent with studies of transcranial magnetic stimulation. [less ▲]

Detailed reference viewed: 53 (6 ULg)
Full Text
Peer Reviewed
See detailConvergentie en divergentie in de Nederlandse standaardtaal. Het stripverhaal Suske en Wiske als casus.
Meesters, Gert ULg

in Nederlandse Taalkunde (2000), 5(2), 164-176

Detailed reference viewed: 99 (1 ULg)
Full Text
Peer Reviewed
See detailElectrochemical study of the uranium exchange by polypyrrole-based resins
Jérôme, Christine ULg; Martinot, Lucien; Jérôme, Robert ULg

in Journal of Applied Polymer Science (1999), 74(14), 3473-3484

Electrochemical control of the binding of uranium ions by redox active polypyrrole resins was studied. The experimental conditions for this binding were optimized with respect to the uranium ions to be ... [more ▼]

Electrochemical control of the binding of uranium ions by redox active polypyrrole resins was studied. The experimental conditions for this binding were optimized with respect to the uranium ions to be fixed, the resin, and the solution. [less ▲]

Detailed reference viewed: 33 (3 ULg)
Full Text
Peer Reviewed
See detailDispense de permis d'urbanisme en Région wallonne (III)
Delnoy, Michel ULg

in Immobilier (1999), 23

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailAnti-Inflammatory and Immunological Effects of Centaurea Cyanus Flower-Heads
Garbacki, Nancy ULg; Gloaguen, Vincent; Damas, Jacques ULg et al

in Journal of Ethnopharmacology (1999), 68(1-3), 235-41

Centaurea cyanus flower-heads are used in European phytotherapy for the treatment of minor ocular inflammations. Different pharmacological experiments (inhibition of carrageenan, zymosan and croton oil ... [more ▼]

Centaurea cyanus flower-heads are used in European phytotherapy for the treatment of minor ocular inflammations. Different pharmacological experiments (inhibition of carrageenan, zymosan and croton oil-induced oedemas, inhibition of plasma haemolytic activity, induction of anaphylatoxin activity) showed that polysaccharides extracted from C. cyanus flower-heads had anti-inflammatory properties and interfered with complement. Moreover, these polysaccharides were found to be mainly composed of galacturonic acid, arabinose, glucose, rhamnose and galactose. [less ▲]

Detailed reference viewed: 123 (7 ULg)
Full Text
Peer Reviewed
See detailDispense de permis d'urbanisme en Région wallonne (II)
Delnoy, Michel ULg

in Immobilier (1999), 22

Detailed reference viewed: 28 (0 ULg)
Full Text
Peer Reviewed
See detailSynthesis and characterization of poly(methyl methacrylate)-block-poly(n-butyl acrylate)-block-poly(methyl methacrylate) copolymers by two-step controlled radical polymerization (ATRP) catalyzed by NiBr2(PPh3)2, 1+
Moineau, Georges; Minet, Michaël; Teyssié, Philippe et al

in Macromolecules (1999), 32(25), 8277-8282

Poly(methyl methacrylate)-block-poly(n-butyl acrylate)-block-poly(methyl methacrylate) triblock copolymers (MnBM) have been synthesized by the sequential controlled radical polymerization (atom transfer ... [more ▼]

Poly(methyl methacrylate)-block-poly(n-butyl acrylate)-block-poly(methyl methacrylate) triblock copolymers (MnBM) have been synthesized by the sequential controlled radical polymerization (atom transfer radical polymerization, ATRP) of n-butyl acrylate (n-BuA) followed by methyl methacrylate (MMA). The polymerization of n-BuA has been first initiated by the difunctional (diethyl meso-2,5-dibromoadipate) initiator in the presence of the NiBr2(PPh3)2 catalyst. After isolation, the α,ω-dibromo poly(n-butyl acrylate) chains have been used as macroinitiators for the polymerization of either n-BuA or MMA leading to chain extension or to the desired triblock copolymers, respectively. The kinetic study of the two-step process has shown that the initiation of the MMA polymerization by the poly(n-BuA) macroinitiator is slow and leads to PMMA outer blocks of broad polydispersity. Differential scanning calorimetry (DSC) and dynamical mechanical analysis (DMA) have confirmed the two-phase morphology of the triblocks. Finally, DMA and tensile testing of these copolymers have emphasized poor mechanical properties in possible relation to the broad polydispersity of the PMMA outer blocks. [less ▲]

Detailed reference viewed: 82 (3 ULg)
See detailMaisons de justice
Dantinne, Michaël ULg

in Journal des Tribunaux (1999), (5947),

Description de l'institution des maisons de justice en Belgique.

Detailed reference viewed: 25 (1 ULg)
Peer Reviewed
See detailApplications of statistical physics to economic and financial topics
Ausloos, Marcel ULg; Vandewalle, Nicolas ULg; Boveroux, P. et al

in Physica A: Statistical Mechanics and its Applications (1999), 274(1-2), 229-240

Problems in economy and finance have started to attract the interest of statistical physicists. Fundamental problems pertain to the existence or not of long-, medium-, short-range power-law correlations ... [more ▼]

Problems in economy and finance have started to attract the interest of statistical physicists. Fundamental problems pertain to the existence or not of long-, medium-, short-range power-law correlations in economic systems as well as to the presence of financial cycles. Methods like the extended detrented fluctuation analysis, and the multi-affine analysis are recalled emphasizing their value in sorting out correlation ranges and predictability. Among spectacular results, the possibility of crash predictions is indicated. The well known financial analyst technique, the so-called moving average, is shown to raise questions about fractional Brownian motion properties. Finally, the (m,k)-Zipf method and the i-variability diagram technique are presented for sorting out short range correlations. Analogies with other fields of modem applied statistical physics are also presented in view of some universal openess. [less ▲]

Detailed reference viewed: 68 (7 ULg)
Peer Reviewed
See detailApplications of statistical physics - Proceedings of the NATO Advanced Research Workshop held at Technical University of Budapest Hungary, 19-22 May 1999 - Preface
Gadomski, A.; Kertesz, J.; Stanley, H. E. et al

in Physica A: Statistical Mechanics and its Applications (1999), 274(1-2), -

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailThermal crosslinking of poly(methyl methacrylate) by reaction of methyl ester and quaternary ammonium salt and application to (meth)acrylate-based TPEs
Tong, Jiang-Dong; Goethals, Eric; Jérôme, Robert ULg

in Journal of Polymer Science. Part A, Polymer Chemistry (1999), 37(23), 4402-4411

Copolymers of N-vinylbenzyl N-methyl pyrrolidinium chloride (VBMPC) and methyl methacrylate, PVBMPC-co-poly(methyl methacrylate) (PMMA), were synthesized by free-radical copolymerization and proved to be ... [more ▼]

Copolymers of N-vinylbenzyl N-methyl pyrrolidinium chloride (VBMPC) and methyl methacrylate, PVBMPC-co-poly(methyl methacrylate) (PMMA), were synthesized by free-radical copolymerization and proved to be prone to crosslinking as a result of the reaction of methyl ester groups with benzyl methyl pyrrolidinium chloride (BMPC) moieties at temperatures higher than 110 °C. When the VBMPC content was lower than 20 wt %, these copolymers were miscible with homo-PMMA. Blends of homo-PMMA and PVBMPC-co-PMMA fully could be cured above 150 °C, when the molecular weight of PMMA exceeded 10,000 and the VBMPC content of the copolymer was higher than 5 wt %. This reaction was carried out to crosslink selectively the PMMA microdomains of PMMA-b-poly(isooctyl acrylate) (PIOA)-b-PMMA (MIM) triblock copolymers to explain the mechanism for the mechanical failure of fully (meth)acrylic thermoplastic elastomers. Comparison of the ultimate tensile properties of MIM block copolymers, when the dispersed PMMA phases and PIOA matrix were crosslinked, led to the conclusion that the ductile failure of the hard PMMA microdomains rather than the elastic failure of the PIOA matrix was the reason for the mechanical failure of MIM triblocks. [less ▲]

Detailed reference viewed: 146 (2 ULg)
Full Text
Peer Reviewed
See detailDeep Imaging of AX J2019+112: The Luminosity of a ``Dark Cluster''
Benítez, Narciso; Broadhurst, Tom; Rosati, Piero et al

in Astrophysical Journal (1999), 527

We detect a distant cluster of galaxies centered on the QSO lens and luminous X-ray source AX J2019+112, a.k.a. the ``Dark Cluster.'' Using deep V and I Keck images and wide-field K[SUB]s[/SUB] imaging ... [more ▼]

We detect a distant cluster of galaxies centered on the QSO lens and luminous X-ray source AX J2019+112, a.k.a. the ``Dark Cluster.'' Using deep V and I Keck images and wide-field K[SUB]s[/SUB] imaging from the New Technology Telescope (NTT), a tight red sequence of galaxies is identified within a radius of 0.2 h[SUP]-1[/SUP] Mpc of the known z=1.01 elliptical lensing galaxy. The sequence, which includes the central elliptical galaxy, has a slope in good agreement with the model predictions of Kodama et al. for z~1. We estimate the integrated rest-frame luminosity of the cluster to be L[SUB]V[/SUB]>=3.2x10[SUP]11[/SUP] h[SUP]-2[/SUP] L[SUB]solar[/SUB] (after accounting for significant extinction at the low latitude of this field), more than an order of magnitude higher than previous estimates. The central region of the cluster is deconvolved using the technique of Magain, Courbin, & Sohy, revealing a thick central arc coincident with an extended radio source. All the observed lensing features are readily explained by differential magnification of a radio-loud active galactic nucleus by a shallow elliptical potential. The QSO must lie just outside the diamond caustic, producing two images; the arc is a highly magnified image formed from a region close to the center of the host galaxy, projecting inside the caustic. The mass-to-light ratio within an aperture of 0.4 h[SUP]-1[/SUP] Mpc is M[SUB]X[/SUB]/L[SUB]V[/SUB]=224[SUP]+112[/SUP][SUB]- 78[/SUB]h(M/L[SUB]V[/SUB])[SUB]solar[/SUB], using the X-ray temperature. The strong lens model yields a compatible value, M/L[SUB]V[/SUB]=372[SUP]+94[/SUP][SUB]- 94[/SUB]h(M/L[SUB]V[/SUB])[SUB]solar[/SUB], whereas an independent weak-lensing analysis sets an upper limit of M/L[SUB]V[/SUB]<520h(M/L[SUB]V[/SUB])[SUB]solar[/SUB], typical of massive clusters. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailThe self-enrichment of galactic halo globular clusters. A clue to their formation?
Parmentier, Geneviève ULg; Jehin, Emmanuel ULg; Magain, Pierre ULg et al

in Astronomy and Astrophysics (1999), 352

We present a model of globular cluster self-enrichment. In the protogalaxy, cold and dense clouds embedded in the hot protogalactic medium are assumed to be the progenitors of galactic halo globular ... [more ▼]

We present a model of globular cluster self-enrichment. In the protogalaxy, cold and dense clouds embedded in the hot protogalactic medium are assumed to be the progenitors of galactic halo globular clusters. The massive stars of a first generation of metal-free stars, born in the central areas of the proto-globular cluster clouds, explode as Type II supernovae. The associated blast waves trigger the expansion of a supershell, sweeping all the material of the cloud, and the heavy elements released by these massive stars enrich the supershell. A second generation of stars is born in these compressed and enriched layers of gas. These stars can recollapse and form a globular cluster. This work aims at revising the most often encountered argument against self-enrichment, namely the presumed ability of a small number of supernovae to disrupt a proto-globular cluster cloud. We describe a model of the dynamics of the supershell and of its progressive chemical enrichment. We show that the minimal mass of the primordial cluster cloud required to avoid disruption by several tens of Type II supernovae is compatible with the masses usually assumed for proto-globular cluster clouds. Furthermore, the corresponding self-enrichment level is in agreement with halo globular cluster metallicities. [less ▲]

Detailed reference viewed: 323 (8 ULg)
Full Text
Peer Reviewed
See detail50 Hz Magnetic Field Exposure Influence on Human Performance and Psychophysiological Parameters: Two Double-Blind Experimental Studies
Crasson, Marion ULg; Legros, Jean-Jacques ULg; Legros, Willy ULg et al

in Bioelectromagnetics (1999), 20(8), 474-86

Two double-blind studies were performed to examine magnetic field (MF) exposure effects and to determine the impact of temporal variation (continuous vs. intermittent exposure) of 100 mu T(rms) 50 Hz MF ... [more ▼]

Two double-blind studies were performed to examine magnetic field (MF) exposure effects and to determine the impact of temporal variation (continuous vs. intermittent exposure) of 100 mu T(rms) 50 Hz MF diurnal exposure on psychological and psychophysiological parameters in healthy humans. Three cephalic exposure sessions of 30-min, i.e., sham, continuous, and intermittent (15 s ON/OFF cycles) MF conditions, were involved. Each subject participated in all sessions, which were spaced at 1-wk intervals. In each session, mood ratings and performance measures were obtained before, during, or after exposure and several electrophysiological data (event-related brain potentials [ERP]) were recorded after each exposure session. These criteria were chosen to evaluate sensory functions as well as automatic and voluntary attentional processes. In experiment 1, 21 healthy male volunteers (20 to 27 years of age) were studied. Ten subjects were exposed at 13:30 h, and 11 subjects were exposed at 16:30 h. Statistically significant changes in the amplitude of ERP were observed after MF exposure in the dichotic listening task, indexing selective attention processes. Eighteen of the 21 original male volunteers took part in experiment 2, undertaken to better understand the results related to information processing involved in selective attention and control for ultradian rhythmicity. Exposure time for all the subjects was at 13:30 h. The analysis of the data again revealed significant amplitude changes of the ERP recorded in the dichotic listening task. Moreover, they demonstrated ERP latency and reaction time slowing in the oddball paradigm, a visual discrimination task after real MF exposure. These results also indicate that a low level 50 Hz MF may have a slight influence on event-related potentials and reaction time under specific circumstances of sustained attention. [less ▲]

Detailed reference viewed: 107 (2 ULg)
Peer Reviewed
See detailAnalytic and clinical evaluation of the Abbott AxSYM cardiac troponin I assay
Elst, K. M. V.; Chapelle, Jean-Paul ULg; Boland, P. et al

in American Journal of Clinical Pathology (1999), 122(6), 745-752

Detailed reference viewed: 28 (0 ULg)
Peer Reviewed
See detailLa loi de protection du malade mental du 26 juin 1990. Présentation et jurisprudence
Boxho, Philippe ULg

in Journal des Juges de Paix = Tijdschrift van de Vrederecters (1999), 12

Detailed reference viewed: 43 (17 ULg)
Full Text
Peer Reviewed
See detailDes éditeurs sans édition. Genèse et structure de l’espace éditorial en Belgique francophone
Durand, Pascal ULg; Winkin, Yves

in Actes de la Recherche en Sciences Sociales (1999), 130

L'édition n'est pas seulement un objet à étudier; elle est aussi un observatoire des pratiques culturelles. En prenant pour terrain d'analyse le marché de la production du livre en Belgique francophone ... [more ▼]

L'édition n'est pas seulement un objet à étudier; elle est aussi un observatoire des pratiques culturelles. En prenant pour terrain d'analyse le marché de la production du livre en Belgique francophone, on se donne le moyen de saisir au concret le rapport d'implication réciproque entretenu par les œuvres avec les vecteurs de leur production et de leur diffusion. Ce rapport, comme ces vecteurs, ont une histoire, particulièrement déterminante et complexe, mais aussi répétitive, dans les territoires belges. Ainsi, il est frappant de constater l’impossibilité, pour l’édition en Belgique, de lui désigner dans l’histoire un moment de naissance à partir duquel elle se serait continûment déployée. C’est, à différents moments, de naissances à répétition qu’il s’agit plutôt et donc, aussi bien, d’extinctions successives. D'où le fait qu'aucune culture éditoriale, au sens le plus littéraire de l'expression, ne se soit véritablement développée en Belgique francophone. Plantin au XVIe siècle édifie certes, à force d’énergie, d’ambition intellectuelle et d’opportunisme politico-religieux, une maison de rayonnement international, mais fait figure d’isolé, mal relayé de surcroît par ses héritiers. Les imprimeurs liégeois et bruxellois, du XVIIIe siècle aux années 1850, inondent l’Europe d’ouvrages contrefaits : ils bloquent pour longtemps le système éditorial dans des routines de reproduction et s’effondreront sous le coup de la Convention franco-belge de 1852. Au XIXe siècle, Casterman fait tourner ses presses au profit des missels et des ouvrages de dévotion : les convoyeurs de la haute littérature attendront. Dans les années 1890, quelques petits éditeurs parviennent à esquisser les contours d’une édition spécifiquement littéraire : tous font très vite faillite quand ils ne s’écroulent pas sous le poids de la censure. Il faudra attendre le nouveau Casterman et Marabout pour que se déploient en Belgique des maisons de rayonnement international, mais dans des genres dominés tels que la bande dessinée et le livre de poche industriel. Des attitudes toutefois se maintiennent, se transmettent peut-être d'une génération à l'autre. L'hypothèse conductrice de l'article est la suivante : de Plantin aux éditeurs littéraires contemporains se construit en Belgique un habitus techniciste, autrement dit une représentation de l'activité éditoriale comme fabrication de livres plutôt que comme production de biens symboliques aptes à circuler sur le marché littéraire à force égale avec ceux que la France, ayant mieux négocié le passage de l'imprimerie à l'édition, produit de son côté. Cette situation n'est pas sans présenter quelques ressources pour l'avenir. A l'heure des mouvements de concentration internationaux, il se pourrait bien en effet que les éditeurs de Belgique, comme ceux d'autres zones marginales, contribuent, du fait de leur fonctionnement apparemment "archaïque", à maintenir la possibilité d'une édition indépendante. [less ▲]

Detailed reference viewed: 227 (32 ULg)
Full Text
Peer Reviewed
See detailThe homeodomain-containing proteins: an update on their interacting partners
Chariot, Alain ULg; Gielen, Jacques; Merville, Marie-Paule ULg et al

in Biochemical Pharmacology (1999), 58

Homeodomain-containing proteins are transcription regulators controlling the coordinated expression of genes involved in development, differentiation, and cellular transformation. They share a highly ... [more ▼]

Homeodomain-containing proteins are transcription regulators controlling the coordinated expression of genes involved in development, differentiation, and cellular transformation. They share a highly conserved 60-amino-acid region (the "homeodomain"), which allows them to bind DNA and modulate the expression of multiple target genes, whose identities remain largely unknown. Although each HOX gene product exhibits in vivo specificity, they harbor very similar DNA-binding affinities in vitro, suggesting that other mechanisms such as protein-protein interactions are critical to modulate their function. In this commentary, we describe the proteins that can interact with the HOX gene products, including newly identified partners such as CREB binding protein and the NF-kappaB/IkappaB-alpha proteins. We also outline the molecular programs that are regulated by the transcriptional complexes involving the HOX gene products and where new pharmacological tools could find interesting targets. [less ▲]

Detailed reference viewed: 25 (3 ULg)
Full Text
Peer Reviewed
See detailVimentin Contributes to Human Mammary Epithelial Cell Migration
Gilles, Christine ULg; Polette, M.; Zahm, J. M. et al

in Journal of Cell Science (1999), 112((Pt 24)), 4615-25

Vimentin expression in human mammary epithelial MCF10A cells was examined as a function of their migratory status using an in vitro wound-healing model. Analysis of the trajectories of the cells and their ... [more ▼]

Vimentin expression in human mammary epithelial MCF10A cells was examined as a function of their migratory status using an in vitro wound-healing model. Analysis of the trajectories of the cells and their migratory speeds by time lapse-video microscopy revealed that vimentin mRNA and protein expression were exclusively induced in cells at the wound's edge which were actively migrating towards the center of the lesion. Actin labeling showed the reorganization of actin filaments in cells at the wound's edge which confirmed the migratory phenotype of this cell subpopulation. Moreover, the vimentin protein disappeared when the cells became stationary after wound closure. Using cells transfected with the vimentin promoter controlling the green fluorescent protein gene, we also demonstrated the specific activation of the vimentin promoter in the migratory cells at the wound's edge. Transfection of the antisense vimentin cDNA into MCF10A cells clearly reduced both their ability to express vimentin and their migratory speed. Taken together, these observations demonstrate that vimentin is transiently associated with, and could be functionally involved in, the migratory status of human epithelial cells. [less ▲]

Detailed reference viewed: 94 (5 ULg)
Full Text
Peer Reviewed
See detailCabergoline in the treatment of hyperprolactinemia: a study in 455 patients.
Verhelst, Johan; Abs, Roger; Maiter, Dominique et al

in Journal of Clinical Endocrinology and Metabolism (1999), 84(7), 2518-22

Cabergoline is a new long-acting dopamine agonist that is very effective and well tolerated in patients with pathological hyperprolactinemia. The aim of this study was to examine, in a very large number ... [more ▼]

Cabergoline is a new long-acting dopamine agonist that is very effective and well tolerated in patients with pathological hyperprolactinemia. The aim of this study was to examine, in a very large number of hyperprolactinemic patients, the ability to normalize PRL levels with cabergoline, to determine the effective dose and tolerance, and to assess the effect on clinical symptoms, tumor shrinkage, and visual field abnormalities. We also evaluated the effects of cabergoline in a large subgroup of patients with bromocriptine intolerance or -resistance. We retrospectively reviewed the files of 455 patients (102 males and 353 females) with pathological hyperprolactinemia treated with cabergoline in 9 Belgian centers. Among these patients, 41% had a microadenoma; 42%, a macroadenoma; 16%, idiopathic hyperprolactinemia; and 1%, an empty sella. The median pretreatment serum PRL level was 124 microg/L (range, 16-26,250 microg/L). A subgroup of 292 patients had previously been treated with bromocriptine, of which 140 showed bromocriptine intolerance and 58 showed bromocriptine resistance. Treatment with cabergoline normalized serum PRL levels in 86% of all patients: in 92% of 244 patients with idiopathic hyperprolactinemia or a microprolactinoma and in 77% of 181 macroadenomas. Pretreatment visual field abnormalities normalized in 70% of patients, and tumor shrinkage was seen in 67% of cases. Side effects were noted in 13% of patients, but only 3.9% discontinued therapy because of side effects. The median dose of cabergoline at the start of therapy was 1.0 mg/week but could be reduced to 0.5 mg/week once control was achieved. Patients with a macroprolactinoma needed a higher median cabergoline dose, compared with those with idiopathic hyperprolactinemia or a microprolactinoma: 1.0 mg/week vs. 0.5 mg/week, although a large overlap existed between these groups. Twenty-seven women treated with cabergoline became pregnant, and 25 delivered a healthy child. One patient had an intended abortion and another a miscarriage. In the patients with bromocriptine intolerance, normalization of PRL was reached in 84% of cases, whereas in the bromocriptine-resistant patients, PRL could be normalized in 70%. We confirmed, in a large-scale retrospective study, the high efficacy and tolerability of cabergoline in the treatment of pathological hyperprolactinemia, leaving few patients with unacceptable side effects or inadequate clinical response. Patients with idiopathic hyperprolactinemia or a microprolactinoma, on average, needed only half the dose of cabergoline as those with macroprolactinomas and have a higher chance of obtaining PRL normalization. Cabergoline also normalized PRL in the majority of patients with known bromocriptine intolerance or -resistance. Once PRL secretion was adequately controlled, the dose of cabergoline could often be significantly decreased, which further reduced costs of therapy. [less ▲]

Detailed reference viewed: 73 (2 ULg)
Full Text
Peer Reviewed
See detailMaximum-likelihood reconstruction with ordered subsets in bone SPECT
Blocklet, Didier; Seret, Alain ULg; Popa, Niculaie et al

in Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine (1999), 40(12), 1978-1984

This study was aimed at determining whether the ordered-subset expectation maximum (OSEM) is more effective than filtered backprojection (FBP) for bone SPECT in the routine clinical context. Methods ... [more ▼]

This study was aimed at determining whether the ordered-subset expectation maximum (OSEM) is more effective than filtered backprojection (FBP) for bone SPECT in the routine clinical context. Methods. Fifty-seven consecutive bone SPECT studies were analyzed. They included pelvic and lumbar spine, thoraco lumbar spine, head and neck, feet and shoulders. A 64-projection SPECT study was acquired over 360° by single-head cameras 2–3h after the injection of 750 MBq 99mTc-methylene diphosphonate. Three observers compared the OSEM and FBP reconstructed images. Results. Streak artifacts, always present with FBP, were rarely generated with the OSEM. When present (n = 24), artifacts associated with negative values near hyperactivities in FBP were not generated with the OSEM in 67% of the cases (n = 16), permitting a satisfactory interpretation of these regions. In half of the other cases (17%, n = 4/24), interpretation was precluded. In only one case did the three observers agree that more hyperactivities were seen with the OSEM. Ninety-six percent of the OSEM pictures were superior or equal to FBP for anatomic resolution and were clearly better in 12% of the cases. The extent of the lesion with the OSEM seemed better or equally defined in 96% and clearly better in 14% of the cases. The low-activity regions were better or equally visualized in all cases and were clearly better seen in 23% of the cases. The quality of the pictures was found to be better or superior with the OSEM in 98% of the cases and definitely better in 65% of the cases. Conclusion: Replacement of FBP by the OSEM in bone SPECT would be beneficial to clinical practice. [less ▲]

Detailed reference viewed: 77 (5 ULg)
Full Text
See detailLes ingénieurs chimistes et leurs instruments: Le cas de l'analyse polarimétrique dans l'industrie sucrière
Melard, François ULg

in Chimie Nouvelle (1999), 17(68), 3008-3011

This article put forward the complexe relations that exists between chemists ingeneers and the development of the Sugar industry in Belgium (in the 19s century). The argument is that the technical ... [more ▼]

This article put forward the complexe relations that exists between chemists ingeneers and the development of the Sugar industry in Belgium (in the 19s century). The argument is that the technical dimension of the implementation of a new chemical protocol for the delivery of sugar beets may be of some importance in order to understand the sociological dimension of contractual relations between actors and the role of scientific expertise in such a context. [less ▲]

Detailed reference viewed: 77 (18 ULg)
Peer Reviewed
See detailL'image du mois. Fermeture percutanée de la communication interauriculaire
Massin, M.; Legrand, Victor ULg; Materne, Pierre ULg et al

in Revue Médicale de Liège (1999), 54(12), 907-8

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailIntérêt de la tomographie a émission de positons dans l'évaluation des tumeurs digestives
Hustinx, Roland ULg; Paulus, Patrick; Daenen, Frédéric ULg et al

in Revue Médicale de Liège (1999), 54(12), 925-30

Imaging and endoscopic techniques have taken an increasing part in the management of gastroenterological disorders. Among these techniques, FDG-PET imaging has emerged as a powerful tool in the management ... [more ▼]

Imaging and endoscopic techniques have taken an increasing part in the management of gastroenterological disorders. Among these techniques, FDG-PET imaging has emerged as a powerful tool in the management of several cancer diseases, including tumors of the digestive tract. In particular, the role of PET for diagnosing and staging recurrent colorectal cancers, and for differentiating mass forming pancreatitis from carcinoma is now well established. In this review, we will briefly discuss the place of PET imaging in the work-up of the tumors of the digestive tract. [less ▲]

Detailed reference viewed: 24 (2 ULg)
Peer Reviewed
See detailMice Transgenic for a Soluble Form of Murine Cytotoxic T Lymphocyte Antigen 4 Are Refractory to Murine Acquired Immune Deficiency Sydrome Development
de Leval, Laurence ULg; Debrus, S.; Lane, P. et al

in Immunology (1999), 98(4), 630-8

Interactions between B and CD4+ T cells are central to the pathogenesis of retrovirus-induced murine acquired immune deficiency virus (MAIDS). Prompted by previous work showing that treatment with ... [more ▼]

Interactions between B and CD4+ T cells are central to the pathogenesis of retrovirus-induced murine acquired immune deficiency virus (MAIDS). Prompted by previous work showing that treatment with cytotoxic T lymphocyte antigen 4 immunoglobulin (CTLA4Ig) partly inhibited the disease, we studied the course of infection in mice deficient for CD28-B7 interactions (mCTLA4-Hgamma1 transgenic mice). Despite a relative viral load identical to that of non-transgenic mice, the transgenic mice did not develop any of the major MAIDS symptoms (i.e. lymphoproliferation and immune anergy). The mCTLA4-Hgamma1 did not however, completely inhibit B-cell activation as indicated by a slight hypergammaglobulinaemia and microscopic blastic transformation. Absence of MAIDS in transgenic mice was associated with much lower levels of both interleukin-4 and interferon-gamma transcripts following viral infection. These results support the theory that the CD28/B7 costimulatory pathway is a critical determinant to MAIDS development. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailFrom living to controlled aluminium alkoxide mediated ring-opening polymerization of (di)lactones, a powerful tool for the macromolecular engineering of aliphatic polyesters
Mecerreyes, David; Jérôme, Robert ULg

in Macromolecular Chemistry and Physics (1999), 200(12), 2581-2590

The aluminium alkoxide mediated ring-opening polymerization of (di)lactones offers a great versatility to the point where the main situations covered by the definition of living and controlled ... [more ▼]

The aluminium alkoxide mediated ring-opening polymerization of (di)lactones offers a great versatility to the point where the main situations covered by the definition of living and controlled polymerizations can be illustrated. Furthermore, substitution of a coordination-insertion mechanism for the traditional anionic polymerization of these monomers results in species of lower reactivity and in a kinetic control of the polyaddition reaction. This control, combined with the use of Al alkoxides of different structures and functionalities, has opened the way to the macromolecular design of a family of biocompatible and biodegradable polyesters, e.g., controlled synthesis of telechelic polymers, block and graft copolymers, and polymers of various molecular architectures. These polymers can be used as such, or as building parts of more sophisticated polymeric assemblies. [less ▲]

Detailed reference viewed: 72 (5 ULg)
Peer Reviewed
See detailPharma-clinics le médicament du mois. La nevirapine. Viramune
Nkoghe, D.; Leonard, Philippe ULg; Moutschen, Michel ULg et al

in Revue Médicale de Liège (1999), 54(12), 948-51

Nevirapine is the first non nucleoside reverse transcriptase inhibitor registered in Belgium and indicated in the treatment of HIV-1 infection. In association with 2 nucleoside analogues, its efficiency ... [more ▼]

Nevirapine is the first non nucleoside reverse transcriptase inhibitor registered in Belgium and indicated in the treatment of HIV-1 infection. In association with 2 nucleoside analogues, its efficiency is similar to a tritherapy with protease inhibitor, particularly in naive patients with low viral load. It has a good tolerance profile and is easy to take. Studies in progress should permit to widen its indications. [less ▲]

Detailed reference viewed: 19 (2 ULg)
Full Text
Peer Reviewed
See detailLa prévention des rechutes adictives
Kempeneers, Philippe ULg

in Revue Francophone de Clinique Comportementale et Cognitive (1999), 4(4), 24-34

Relapse prevention (RP) is a model of therapeutic intervention started in the field of substance abuses but applicable to all addictive behaviors. This papper first specifies the concept of addiction ... [more ▼]

Relapse prevention (RP) is a model of therapeutic intervention started in the field of substance abuses but applicable to all addictive behaviors. This papper first specifies the concept of addiction. Follows a description of the principles of RP concerning the relapse process and a specification of RP as a clinical and theorical model. Finally there is a more detailed presentation of several mechansisms characteristics of addictive relapses as well as the modes of treatment the RP offers [less ▲]

Detailed reference viewed: 104 (9 ULg)
Peer Reviewed
See detailRepeatability of the Intensity Dependence of Cortical Auditory Evoked Potentials in the Assessment of Cortical Information Processing
Sandor, P. S.; Roon, K. I.; Ferrari, M. D. et al

in Cephalalgia : An International Journal of Headache (1999), 19(10), 873-9

Data on repeatability and optimal settings are needed when studying the influence of drugs on the intensity dependence of auditory evoked cortical potentials (IDAP). IDAP was recorded at intervals of 1, 2 ... [more ▼]

Data on repeatability and optimal settings are needed when studying the influence of drugs on the intensity dependence of auditory evoked cortical potentials (IDAP). IDAP was recorded at intervals of 1, 2, and 24 h at two centers in 22 healthy volunteers. Settings were modified to compare fixed versus randomly varied stimulus repetition rate, as well as 30 Hz and 100 Hz low pass filters. Repeatability was assessed for different intervals and different settings. Group means did not differ between centers, the 2-h or 24-h retest, or when using different settings. We observed an order effect for the 1 h retest. Fixed repetition rate and the 30 Hz filter improved repeatability with still high intraindividual variability. IDAP group means can be compared between centers for retest intervals of 2 h and 24 h and different settings. Variability is too large to compare individuals. [less ▲]

Detailed reference viewed: 22 (0 ULg)
Full Text
Peer Reviewed
See detailApplication of Supercritical Carbon Dioxide for the Preparation of a Piroxicam-Beta-Cyclodextrin Inclusion Compound
Van Hees, Thierry ULg; Piel, Géraldine ULg; Evrard, Brigitte ULg et al

in Pharmaceutical Research (1999), 16(12), 1864-70

PURPOSE: Piroxicam is a poorly soluble NSAID, whose solubility is enhanced when included into beta-cyclodextrin. The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 ... [more ▼]

PURPOSE: Piroxicam is a poorly soluble NSAID, whose solubility is enhanced when included into beta-cyclodextrin. The preparation of a piroxicam-beta-cyclodextrin inclusion compound using supercritical CO2 was investigated. METHODS: The solubility and the stability of piroxicam in supercritical CO2 were determined. Then, the influence of the temperature, the pressure and the time of exposure on the inclusion rate was studied. RESULTS: The solubility of piroxicam varied over a wide range depending on the temperature and pressure (from 0.006 to 1.500 mg/g of CO2). The temperature and the time of exposure had a great influence on the inclusion yield, while pressure did not and a complete inclusion was achieved by keeping a physical mixture of piroxicam and beta-cyclodextrin (1:2.5 mol/mol) for 6 hours at 150 degrees C and 15 MPa of CO2. This complex was characterized by Differential Scanning Calorimetry, differential solubility and Fourier Transform Infrared Spectrometry. CONCLUSIONS: Supercritical carbon dioxide may prove to be a novel useful complexation method of drugs into beta-cyclodextrin. [less ▲]

Detailed reference viewed: 76 (2 ULg)
Peer Reviewed
See detailPharma-clinics comment je traite ... une infection par le VIH. III. Les inhibiteurs non nucléosidiques de la transcriptase inverse
Nkoghe, D.; Moutschen, Michel ULg; Leonard, Philippe ULg et al

in Revue Médicale de Liège (1999), 54(12), 909-11

Non nucleoside reverse transcriptase inhibitors (NNRTI) are a new arm in the treatment of the HIV infection. They inhibit the replication by direct non competitive binding to the enzyme, and do not ... [more ▼]

Non nucleoside reverse transcriptase inhibitors (NNRTI) are a new arm in the treatment of the HIV infection. They inhibit the replication by direct non competitive binding to the enzyme, and do not require phosphorylation. The fast emergence of resistance in monotherapy obliges to use them in a triple association. The 103 mutation confers a cross-resistance. The most common adverse event is rash. Association with nucleoside analogues is additive or even synergistic. They are metabolized by the cytochrome P450. Within a combined therapy, their efficiency is comparable to protease inhibitors, notably in patients with low viral load. [less ▲]

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailDispense de permis d'urbanisme en Région wallonne (I)
Delnoy, Michel ULg

in Immobilier (1999), 21

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailTranscription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp
Kausel, Gudrun; Vera, María Inés; San Martin, Rody et al

in Journal of Cellular Biochemistry (1999), 75(4), 598-609

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and ... [more ▼]

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat. J. Cell. Biochem. 75:598-609, 1999. © 1999 Wiley-Liss, Inc. [less ▲]

Detailed reference viewed: 19 (4 ULg)
Peer Reviewed
See detailEvolution on trees: a brief review about the extinctions of species, and the case of genocide in a Darwinistic punctuated equilibrium evolution model
Ausloos, Marcel ULg; Kramer, M.; Vandewalle, Nicolas ULg

in Physica A: Statistical Mechanics and its Applications (1999), 273(1-2), 33-45

Numerical and theoretical investigations of species evolution in presence of extinction rules are recalled in the framework of punctuated equilibrium evolution models. Previous results, on the Bak-Sneppen ... [more ▼]

Numerical and theoretical investigations of species evolution in presence of extinction rules are recalled in the framework of punctuated equilibrium evolution models. Previous results, on the Bak-Sneppen model are mentioned together with extensions taking into account a variable number of species. Conditions for limited growth due to species interactions, external fields, or intrinsic population density conditions have already been considered. An original genocide model is presented as a step towards describing effects due to the extinction of species. Critical exponents for (i) the avalanche distribution. (ii) the teady-state approach, and (iii) the tree size are given. A mean-field approximation is shown to be quite accurate for describing the critical state. The screening of species from the evolution process is not numerically studied. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailInflammatory Effect of Intratracheal Instillation of Ultrafine Particles in the Rabbit: Role of C-Fiber and Mast Cells
Nemmar, A.; Delaunois, Annie ULg; Nemery, B. et al

in Toxicology and Applied Pharmacology (1999), 160(3), 250-61

The effects of ultrafine polystyrene carboxylate-modified (fluorospheres) on inflammatory processes are being investigated in rabbit lungs. One milliliter of sterile NaCl (0.9%) containing 4 mg of ... [more ▼]

The effects of ultrafine polystyrene carboxylate-modified (fluorospheres) on inflammatory processes are being investigated in rabbit lungs. One milliliter of sterile NaCl (0.9%) containing 4 mg of ultrafine particles (UFP) was intratracheally instilled into anesthetized rabbits. The control animals were only instilled with sterile NaCl (0.9%). Twenty hours after being instilled, the rabbits were killed and their lungs were excised and then tracheally perfused with phosphate-buffered physiological solution (PBS). The lung effluents, collected from small holes made in the pleura, were analyzed for substance P (SP) and histamine content by radioimmunoassay (RIA) methods, after administration of drugs. In addition, in other groups of rabbits, the lung wet/dry (W/D) weight ratio was monitored, as were the cellular and protein contents in bronchoalveolar lavage (BAL). Electron microscopy examination was also performed. In tracheally superfused experiments, UFP induced a significant enhancement of both SP and histamine releases after administration of capsaicin (10(-4) M), to stimulate C-fiber, and carbachol (10(-4) M), a cholinergic agonist. A significant increase in histamine release was also recorded in the UFP-instilled group following the administration of both SP (10(-6) M) plus thiorphan (10(-5) M) and compound 48/80 (C48/80) (10(-3) M) to stimulate mast cells. In addition, the BAL fluid analysis of UFP groups showed an influx of neutrophils and an increase in total protein concentration. An increase in the lung WW/DW ratio was also recorded. Both epithelial and endothelial injuries were observed in the lungs of UFP-instilled rabbits. The pretreatment of rabbits in vivo with a mixture of either SR 140333 and SR 48368, a tachykinin NK(1) and NK(2) receptor antagonist, or a mixture of terfenadine and cimetidine, a histamine H(1) and H(2) receptor antagonist, prevented UFP- induced neutrophil influx and increased total proteins and lung WW/DW ratio. Therefore, it can be concluded that chemicaly inert, electrically charged UFP induce a pulmonary inflammatory process during which the release of SP and histamine from C-fibers and mast cells was enhanced after various stimuli. These latter mediators can also modulate the inflammatory process. [less ▲]

Detailed reference viewed: 34 (1 ULg)
Full Text
See detailLa "culture médiatique" au XIXe siècle
Durand, Pascal ULg

in Les Dossiers de l'ORM (1999), 6

Detailed reference viewed: 57 (12 ULg)
Full Text
Peer Reviewed
See detailInfluence of Melting and Rheological Properties of Fatty Binders on the Melt Granulation Process in a High-Shear Mixer
Evrard, Brigitte ULg; Amighi, K.; Beten, D. et al

in Drug Development & Industrial Pharmacy (1999), 25(11), 1177-84

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely ... [more ▼]

The preparation of granules by melt granulation was investigated using a laboratory-scale high-shear mixer (Pellmix PL 1/8) and binary mixtures containing lactose and different lipidic binders, namely, Compritol 888, Cutina HR, or Precirol ATO5. [less ▲]

Detailed reference viewed: 32 (0 ULg)
Full Text
Peer Reviewed
See detailIdentification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products
Meyer, Gilles; Bare, O.; Thiry, Etienne ULg

in Journal of General Virology (The) (1999), 80(Pt 11), 2849-59

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 ... [more ▼]

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 glycoprotein gH was sequenced. A high degree of conservation was found between BHV-1 and BHV-5 deduced gH amino acid sequences (86. 4%), which is also observed for all alphaherpesvirus gH sequences. Transcriptional analysis revealed a 3.1 kb mRNA as the specific gH transcript which was detected 2 h post-infection (p.i.). Twelve out of twenty-one MAbs directed against BHV-1 gH immunoprecipitated a 108-110 kDa glycoprotein, which was then designated BHV-5 gH. Synthesis and intracellular processing of BHV- 5 gH was analysed in infected MDBK cells using gH cross-reacting MAbs. Glycoprotein gH was expressed as a beta-gamma protein, detected by radioimmunoprecipitation as early as 3 h p.i. Glycosylation studies indicated that BHV-5 gH contains N-linked carbohydrates which are essential for the recognition of the protein by the MAbs. This suggests that N-linked glycans are involved in protein folding or are targets for the gH cross-reacting MAbs. Plaque- reduction neutralization assays showed that at least one BHV-1 gH antigenic domain is lacking in BHV-5 which may possibly relate to in vivo differences in virus tropism. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailRégénération osseuse: on touche au but!
Mainjot, Amélie ULg

in Journal du Dentiste, cahiers scientifiques (1999)

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailA Cell Type-Specific and Gap Junction-Independent Mechanism for the Herpes Simplex Virus-1 Thymidine Kinase Gene/Ganciclovir-Mediated Bystander Effect
Princen, Frederic; Robe, Pierre ULg; Lechanteur, Chantal ULg et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (1999), 5(11), 3639-44

Tumor cells expressing the herpes simplex virus type 1 thymidine kinase (HSV-tk) gene are killed by nucleoside analogues such as ganciclovir (GCV). GCV affects not only the cells expressing HSV-tk but ... [more ▼]

Tumor cells expressing the herpes simplex virus type 1 thymidine kinase (HSV-tk) gene are killed by nucleoside analogues such as ganciclovir (GCV). GCV affects not only the cells expressing HSV-tk but also neighboring cells that do not express the gene; this phenomenon commonly is called "bystander effect." GCV metabolites transfer via gap junctional intercellular communication (GJIC) accounts for the bystander effect in different cell lines, but other mechanisms have also been described. In this study, we analyzed the mechanisms of the bystander effect in two cell lines exhibiting different capacities of communication (DHD/K12 and 9L). The 9L cells exhibited a very good bystander effect, which was completely blocked by a long-term inhibitor of GJIC, 18 alpha-glycyrrhetinic acid. DHD/K12 cells exhibited a moderate bystander effect that was not abolished by 18 alpha-glycyrrhetinic acid or 1-octanol, another strong inhibitor of GJIC. Interestingly, we also observed a bystander effect in cultures where HSV-tk-expressing DHD/K12 cells were physically separated from their untransfected counterparts but grown in the same medium. Moreover, the transfer of filtered conditioned medium from GCV-treated HSV-tk-expressing DHD/K12 cells to DHD/K12 parental cells induced a decrease of survival in a concentration-dependent manner, suggesting that the bystander effect in this cell line was mediated by a soluble factor. [less ▲]

Detailed reference viewed: 38 (3 ULg)
Peer Reviewed
See detailCrise familiale et tribunal : quelle urgence pour l'expert?
Malchair, Alain ULg

in Revue Française et Francophone de Psychiatrie et de Psychologie Médicale (1999), 32

Detailed reference viewed: 37 (14 ULg)
Peer Reviewed
See detailChirurgie, radiotherapie ou hormonotherapie dans le traitement du cancer de la prostate
Bonnet, Pierre ULg; Coppens, Luc ULg; Andrianne, Robert ULg et al

in Revue Médicale de Liège (1999), 54(11), 875-85

Prostatic cancer (PC) became the first diagnosed cancer in western men and is the second leading cause of cancer death in men. Wide utilisation of serum PSA and free PSA measurements, identifies patients ... [more ▼]

Prostatic cancer (PC) became the first diagnosed cancer in western men and is the second leading cause of cancer death in men. Wide utilisation of serum PSA and free PSA measurements, identifies patients requiring transrectalultrasonography (TRUS) and TRUS guided biopsies. Most prostatic cancers diagnosed today are locally limited and may be treated by radical surgery or radiotherapy. In case of disseminated disease, hormonal manipulations remain the treatment of choice. In that field, many new drugs have been designed to allow medical castration with less complications, especially regarding sexual potency. [less ▲]

Detailed reference viewed: 186 (20 ULg)
Peer Reviewed
See detailHigh Concentrations of Histamine Stimulate Equine Polymorphonuclear Neutrophils to Produce Reactive Oxygen Species
Benbarek, H.; Mouithys-Mickalad, Ange ULg; Deby-Dupont, G. et al

in Inflammation Research (1999), 48(11), 594-601

OBJECTIVE AND DESIGN: Because high concentrations of histamine are locally released in inflammation, we investigated the effects of supraphysiological doses of histamine on the production of reactive ... [more ▼]

OBJECTIVE AND DESIGN: Because high concentrations of histamine are locally released in inflammation, we investigated the effects of supraphysiological doses of histamine on the production of reactive oxygen species (ROS) by neutrophils. MATERIALS AND METHODS: Isolated equine neutrophils were activated by 10(-4) to 5 x 10(-3) M histamine. The production of ROS and free radicals was estimated by luminol-enhanced chemiluminescence (CL) and electron spin resonance (ESR) with spin trapping technique. In this model of histamine-stimulated neutrophils, we tested the antagonists of H1 and H2 histamine receptors, the role of Ca2+ and Mg2+, the role of staurosporine and pertussis toxin (inhibitors of protein kinase C and proteins G) and the effects of superoxide dismutase, catalase, hydroxyl radical scavengers (phenylalanine and mannitol) and N(G)-monomethyl-L-arginine (L-NMMA), inhibitor of NO-synthase. RESULTS: Histamine (from 10(-5) to 10(-3) M) stimulated neutrophils to produce CL and ESR signals characterized by spin adducts of superoxide anion and/or hydroxyl radicals. The CL response was inhibited by 10(-4) and 10(-3) M H1 receptor antagonists (promethazine, pyrilamine, and diphenhydramine), by Ca2+ and Mg2+ depletion and by 10 nmoles staurosporine. CL was partially inhibited by pertussis toxin (4 microg/ mL). The ESR signals were practically suppressed by pyrilamine (an H1 receptor antagonist) and superoxide dismutase, and partially inhibited by catalase, hydroxyl radical scavengers and L-NMMA (respectively 59, +/- 30% and 68% inhibition). CONCLUSIONS: High concentrations of histamine stimulated the neutrophils to product ROS and free radicals via H1 receptors and the NADPH-oxidase pathway. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailAirway Mast-Cell Activation in Asthmatics Is Associated with Selective Sputum Eosinophilia
Bettiol, Jane; Radermecker, Maurice ULg; Sele, Jocelyne ULg et al

in Allergy (1999), 54(11), 1188-93

BACKGROUND: Tryptase is a serine endoprotease selectively released from mast cells. Although mast cells are known to be activated after experimental allergic provocation, their role in naturally occurring ... [more ▼]

BACKGROUND: Tryptase is a serine endoprotease selectively released from mast cells. Although mast cells are known to be activated after experimental allergic provocation, their role in naturally occurring asthma is still debated. METHODS: We have investigated the levels of tryptase in the whole induced sputum collected from 51 asthmatics (31 atopic and 20 intrinsic) seen in our outpatient clinic and 22 normal nonatopic healthy volunteers. Tryptase was measured by a new immunoassay based on B12 monoclonal antibody recognition of total tryptase (UniCAP System, Pharmacia) with a sensitivity of 1 ng/ml. RESULTS: While being below the threshold of detection in all normal volunteers, tryptase was detectable in the sputum from 9/51 asthmatics (18%) including five atopic and four intrinsic asthma cases. In these patients, among whom three were asymptomatic asthmatics, the values ranged between 1 and 6.1 ng/ml. The asthmatics with detectable sputum tryptase had greater sputum eosinophil counts (P<0.05) but lower neutrophil counts (P<0.05) than those in whom tryptase was undetectable. When compared to control subjects, asthmatics without tryptase had still greater eosinophil counts (P<0.0001) but also raised neutrophil counts (P<0.05). No significant difference could be found between asthmatics with tryptase and those without tryptase with respect to the age, the baseline lung function, the methacholine bronchial responsiveness, and the frequency of treatment with inhaled steroids. CONCLUSIONS: With the UniCAP System, tryptase was detectable in the sputum from 18% of asthmatics irrespective of atopy and current symptoms. Asthmatics with tryptase appeared to have a selective increase in sputum eosinophil counts while those without tryptase displayed a mixed sputum granulocyte infiltration with raised eosinophil and neutrophil counts. [less ▲]

Detailed reference viewed: 43 (3 ULg)
Peer Reviewed
See detailExperimental Infection of Specific Pathogen-Free New Zealand White Rabbits with Five Strains of Amyxomatous Myxoma Virus
Marlier, Didier ULg; Cassart, Dominique ULg; Boucraut-Baralon, C. et al

in Journal of Comparative Pathology (1999), 121(4), 369-84

Myxomatosis is a specific disease of the European rabbit (Oryctolagus cuniculus) due to a virus belonging to the genus Leporipoxvirus. Forty-seven years after its deliberate introduction into Europe, the ... [more ▼]

Myxomatosis is a specific disease of the European rabbit (Oryctolagus cuniculus) due to a virus belonging to the genus Leporipoxvirus. Forty-seven years after its deliberate introduction into Europe, the clinical aspects and the epizootiology of myxomatosis have changed. Two forms (nodular and amyxomatous) of the disease have been identified to date. A comparative study was made of the clinical signs, pathogenesis and gross lesions observed in male specific pathogen-free New Zealand White rabbits inoculated with five strains of amyxomatous myxoma virus. All five strains induced the characteristic amyxomatous myxomatosis clinical syndrome with clinical signs that differed only in intensity. The varying clinical intensity, together with the results of virological examination question the virulence of at least three of the five strains. Genomic analysis confirmed that the five strains came from the Lausanne strain introduced in 1952 in France and not from an unnoticed introduction of a Californian strain of myxoma virus. No link was found between the amyxomatous myxoma virus strains and the SG33 vaccine strain. 1999 Harcourt Publishers Ltd. [less ▲]

Detailed reference viewed: 41 (5 ULg)
Full Text
Peer Reviewed
See detailMiscibility of poly(vinylidene fluoride) and poly(methylmethacrylate-co-zinc polyacrylate) ionomers
Moussaif, Noureddin; Jérôme, Robert ULg

in Polymer (1999), 40(24), 6831-6839

A random copolymer of methyl methacrylate and 5.7 mol.% of acrylic acid has been neutralized by zinc cation to different extents in order to study the effect of zinc carboxylate pendant groups on the ... [more ▼]

A random copolymer of methyl methacrylate and 5.7 mol.% of acrylic acid has been neutralized by zinc cation to different extents in order to study the effect of zinc carboxylate pendant groups on the miscibility of poly(methyl methacrylate) (PMMA) and poly(vinylidene fluoride) (PVDF). The interaction parameter (χ) has been calculated from the experimental depression of the PVDF melting point. χ is minimum at zinc carboxylate content of 2.8 mol.%. The dynamic mechanical analysis of the PVDF-PMMA ionomer binary blends does not agree with the additivity rule of the properties, consistently with the phase morphology that changes with composition. Blends are indeed amorphous as long as the PVDF content does not exceed 40 wt.%, otherwise, semicrystalline PVDF coexists with an amorphous mixed PVDF/copolymer phase. [less ▲]

Detailed reference viewed: 54 (2 ULg)
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Le cerebral salt wasting syndrome: a propos d'un cas
Llabres, V.; Canivet, Jean-Luc ULg; Hennuy, V. et al

in Revue Médicale de Liège (1999), 54(11), 850-853

A 75 year old woman was found to have a posterior cerebellar lesion which after surgical removal was shown to be a meningioma. Her postoperative course was complicated by a MRSA meningitis and on day 23 ... [more ▼]

A 75 year old woman was found to have a posterior cerebellar lesion which after surgical removal was shown to be a meningioma. Her postoperative course was complicated by a MRSA meningitis and on day 23 after resection, a polyuria up to 11.7 1/24 h became apparent. The diagnosis of cerebral wasting syndrome (CSWS) was made based on biological and clinical features such as an excessive natriuresis (143 mmol/l) resulting in hyponatremia (130 mmol/l) and an osmolarity higher in urine than in blood. A low central venous pressure and a low wedge pressure confirming a volumic depletion indicated the diagnosis of CSWS. This syndrome has marked similarities with the Inappropriate Secretion of Antidiuretic Hormone Syndrome (SIADH) in terms of biological finding with regards to clinical context and presentation. Without an adequate assessment, a patient with CSWS may be misdiagnosed as SIADH. However recognition is important, as water restriction which is part of SIADH treatment, is detrimental to patients with CSWS and can possibly be lethal. [less ▲]

Detailed reference viewed: 36 (2 ULg)
Peer Reviewed
See detailStudy of the protective effects of hyperimmune immunoglobulins G and M against endotoxin in mice and rats
Nys, Monique ULg; Damas, Jacques ULg; Damas, Pierre ULg et al

in Medical Microbiology & Immunology (1999), 188(2), 55-64

We prepared solutions of human IgM and IgG to various lipopolysaccharide (LPS) species. These were then tested, along with solutions of non-LPS specific human IgG or IgM, for their ability to confer ... [more ▼]

We prepared solutions of human IgM and IgG to various lipopolysaccharide (LPS) species. These were then tested, along with solutions of non-LPS specific human IgG or IgM, for their ability to confer passive immunity against experimental endotoxemia in two animal models. The immunoglobulins were first tested for an effect on the lethality induced by seven different LPSs in actinomycin-D sensitized mice, or by three different bacteria in normal mice. When the immunoglobulins were administered 1 h before challenge, a small protective effect was observed. This protection was dependent upon both the anti-LPS agent, the chemical composition of the LPS, or the strain of gram-negative bacteria used for injection. The anti-LPS IgM and IgG preparations reduced the mortality induced by Escherichia coli but not by Serratia marcescens or Klebsiella pneumoniae, indicating protection by strain-specific antibodies. When the antibodies were preincubated with LPS or bacteria for 30 min before administration, almost complete protection was seen. The influence of these immunoglobulin preparations or of human albumin (as a control) on the hypotensive and vascular-permeabilizing effects of LPS in rats was then studied. A dose-dependent inhibitory effect was observed with IgG preparations and albumin. At 200 mg/kg, anti-LPS IgG reduced the effects of LPS, while at 400 mg/kg, both anti-LPS and normal IgG preparations showed protection, as did human albumin used at the same dose. The IgM-enriched preparation worsened the initial hypotensive phase after LPS, whereas the anti-LPS IgM significantly reduced the second phase of the hypotension, but only at the largest dose of 400 mg/kg. In this second model using the rat, a clear difference between the activity of IgG and IgM was thus observed. We conclude that pretreatment with human immunoglobulins from large plasma pools modestly, but significantly, attenuated the effects of murine and rat Gram-negative sepsis, but that protection was incomplete. Our results suggest that single regimen intervention strategies may not be sufficient to influence the course of the disease. [less ▲]

Detailed reference viewed: 62 (0 ULg)
Full Text
Peer Reviewed
See detailLa “culture médiatique” au XIXe siècle. Essai de définition-périodisation
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999)

Detailed reference viewed: 201 (5 ULg)
Full Text
See detailL'approche expérimentale est-elle adaptée à l'évaluation des actions d'éducation pour la santé ?
Liesse, Anne; Vandoorne, Chantal ULg

in Education Santé (1999), (143), 5-8

Detailed reference viewed: 89 (21 ULg)
Full Text
Peer Reviewed
See detailMid-term follow-up after multiple system organ failure following cardiac surgery in children.
Heying, R.; SEGHAYE, Marie-Christine ULg; Grabitz, R. G. et al

in Acta Paediatrica (1999), 88(11), 1238-43

Multiple system organ failure after cardiac surgery in children is a severe complication with unknown mid- and long-term sequelae. We therefore evaluated 11 children (aged 20-126 mo, median: 67 mo) having ... [more ▼]

Multiple system organ failure after cardiac surgery in children is a severe complication with unknown mid- and long-term sequelae. We therefore evaluated 11 children (aged 20-126 mo, median: 67 mo) having survived multiple system organ failure after cardiac operations for congenital cardiac defects in a cross-sectional follow-up study 12-76 mo (median: 32 mo) after surgery. Clinical and laboratory examinations included cardiac, pulmonary, renal, hepatic, neurological and psychological function tests. All patients had adequate cardiac function. Lung mechanics were abnormal in three children and glomerular renal function was abnormal in two patients. Slight elevation of gamma-glutamyl transpeptidase and coagulation factor deficiency was present in six and seven patients, respectively (five of whom had undergone the Fontan operation). Severe neurological sequelae such as diplegia (n = 1) and mental retardation (n = 1) were observed in two patients. In addition, five children presented delayed motor, graphomotor and/or speech development. Two children were found to have abnormal intelligence. We conclude that with the exception of neurological impairment, mid-term sequelae of multiple system organ failure after cardiac surgery in children are mild. However, longer follow-up using an appropriate control group is mandatory. [less ▲]

Detailed reference viewed: 16 (1 ULg)
Peer Reviewed
See detailInfluence of human anti-lipopolysaccharide immunoglobulins on tissue distribution and clearance of lipopolysaccharide in rats
Nys, Monique ULg; Damas, Jacques ULg; Damas, Pierre ULg et al

in Medical Microbiology & Immunology (1999), 188(2), 65-71

To examine the influence of passive immunization on the biological fate of injected lipopolysaccharide (LPS), we used a human IgG preparation (anti-LPS IgG) rich in antibodies to a large panel of smooth ... [more ▼]

To examine the influence of passive immunization on the biological fate of injected lipopolysaccharide (LPS), we used a human IgG preparation (anti-LPS IgG) rich in antibodies to a large panel of smooth and rough purified LPS extracts as well as a normal IgG preparation (standard IgG). Our approach was to compare the uptake of 125I-labeled LPS by the tissues of saline or IgG-treated rats. After intravenous injection, one fraction of 125I-labeled Escherichia coli O55:B5 LPS is rapidly taken up by tissues, while another fraction remained in the blood. Uptake of 125I-labeled LPS was principally observed into the liver and spleen. In rats treated prophylactically with standard IgG, these tissues accumulated significantly larger amount of LPS than the tissues of rats treated with anti-LPS IgG. Nevertheless, both IgG preparations increased the specific binding of LPS by the liver and spleen. High levels of homologous unlabeled LPS decreased the uptake of LPS by the liver, presumably by occupying tissue receptors, whereas in the presence of E. coli O127:B8 LPS, an increase of the uptake of 125I-labeled LPS by the liver and lungs was observed. The pharmacokinetics and tissue distribution of LPS-IgG complexes pre-formed in vitro were compared. In the presence of standard IgG, a unexpected increase of the uptake of LPS by the tissues was recorded, whereas LPS-anti-LPS IgG complexes decreased the binding of 125I-labeled LPS to the tissues. On the other hand, the vascular effects induced by LPS did not appear to be modified in rats pretreated with either IgG preparation. In conclusion, although passive immunization against LPS slightly modified the uptake and clearance of LPS, neither in vitro nor in vivo formation of LPS-anti-LPS IgG complexes afforded a very significant protection against the toxic effects of LPS. [less ▲]

Detailed reference viewed: 22 (0 ULg)
Full Text
Peer Reviewed
See detailLa fundación vanguardista de la traducción
Willson, Patricia ULg

in Espacios de crítica y producción (1999), (25), 54-58

This article examines and contradicts some of the stereotypes in the field of criticism about the image of Borges as translator. From the collation of his translations of works by Faulkner, Joyce and ... [more ▼]

This article examines and contradicts some of the stereotypes in the field of criticism about the image of Borges as translator. From the collation of his translations of works by Faulkner, Joyce and Woolf, the relationship between these versions and his first essays and fictions is shown, especially the ones with a «criollista» tone. [less ▲]

Detailed reference viewed: 250 (16 ULg)
Full Text
Peer Reviewed
See detailAge-dependence of the anticonvulsant effects of the GABA uptake inhibitor tiagabine in vitro
Sabau, A; Frahm, C; Pfeiffer, M et al

in European Journal of Pharmacology (1999), 383(3), 259-266

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailAntiplatelet Activity of Clopidogrel in Coronary Artery Bypass Graft Surgery Patients
David, Jean-Louis ULg; Limet, Raymond ULg

in Thrombosis and Haemostasis (1999), 82(5), 1417-21

Clopidogrel is a recently introduced platelet ADP receptor antagonist, belonging to the thienopyridine derivatives, like its analogue ticlopidine. Its potential advantage is to be safer than ticlopidine ... [more ▼]

Clopidogrel is a recently introduced platelet ADP receptor antagonist, belonging to the thienopyridine derivatives, like its analogue ticlopidine. Its potential advantage is to be safer than ticlopidine. At 75 mg/od clopidogrel significantly inhibits platelet aggregation in ambulatory patients with symptomatic atherosclerotic disease and it prevents the recurrence of ischemic events more efficiently than aspirin. Its adequate dose in more acute situations remained to be determined. Therefore, sixty two patients with coronary artery disease were randomly assigned in four groups treated, within 24 h after coronary artery bypass graft, by clopidogrel 50 mg/od, 75 mg/od or 100 mg/od or by ticlopidine 250 mg/bid which was considered as the reference. The tolerance of clopidogrel was fairly good during the whole period of the study. Bleeding time and ex-vivo platelet aggregation induced by ADP 2 microM and 5 microM were performed at day -1, +9 and +28 after surgery. Like ticlopidine, the three dose levels of clopidogrel significantly inhibited ex-vivo platelet activity and prolonged the bleeding time at day 28. However, unlike ticlopidine, the inhibitory effects of clopidogrel were not significant at day 9, especially with 75 mg/od, a dose which was found to significantly protect patients in a chronic situation. Hence, although the clinical outcome for patients included in this limited study was the same in the four groups, these results suggest that the dose regime of clopidogrel should be more extensively investigated during the early period following coronary artery bypass graft, facing an overproduction of young and hyperreactive platelets. By analogy, the dose regime should be also investigated in other situations with an acute risk of arterial thrombotic occlusion. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailConfrontation anatomo-clinique. A propos d'un cas de carcinome renal a cellules claires
Daloze, A.; Delbecque, Katty ULg; Delvenne, Philippe ULg et al

in Revue Médicale de Liège (1999), 54(11), 859-63

The authors report the case of a patient with a history of hypertension and multiple intracerebral hemorrhages who was found at post mortem examination to have a renal cell carcinoma. The relationship ... [more ▼]

The authors report the case of a patient with a history of hypertension and multiple intracerebral hemorrhages who was found at post mortem examination to have a renal cell carcinoma. The relationship between renal carcinoma and hypertensive intracerebral hemorrhages is discussed. [less ▲]

Detailed reference viewed: 40 (2 ULg)
Full Text
Peer Reviewed
See detailEvaluation of frequency and type of errors detected by a computerized record and verify system during radiation treatment
Barthelemy, Nicole ULg; Sabatier, Jacques; Dewé, Walthère ULg et al

in Radiotherapy & Oncology (1999), 53(2), 149-54

Background: Computerized record and verify systems (RVS) have been introduced to improve the precision of radiation treatment delivery. These systems prevent the delivery of ionizing radiations when the ... [more ▼]

Background: Computerized record and verify systems (RVS) have been introduced to improve the precision of radiation treatment delivery. These systems prevent the delivery of ionizing radiations when the settings of the treatment machine do not match the intended parameters within some maximal authorized deviation. Purpose: To assess the potential alteration of the frequency of errors associated with the use of RVS during radiation treatment delivery. Materials and methods: The software of the RVS was altered in order to record the settings actually used for radiation treatment delivery whereas the verification function was suppressed. At the end of the study period, the settings used during daily administration of radiation treatment were compared to the parameters recorded in the RVS using the computer. They were also compared with the planned ones written in the patient treatment chart. Results: Out of the 147 476 parameters examined during the study period, 678 (0.46%) were set erroneously. At least one error occurred in 628 (3.22%) of the 19 512 treated fields. An erroneous parameter was introduced in the RVS memory in 22 (1.17%) of the 1885 fields. Conclusions: RVS has the potential to improve precision of radiation treatment delivery by detecting a significant number of setting errors. However, excessive confidence in RVS could lead to repeated errors as there is a potential for the entry of erroneous parameters into the RVS memory. [less ▲]

Detailed reference viewed: 63 (8 ULg)
Peer Reviewed
See detailA common mutation (epsilon1267delG) in congenital myasthenic patients of Gypsy ethnic origin
Abicht, A.; Stucka, R.; Karcagi, V. et al

in Neurology (1999), 53(7), 1564-9

OBJECTIVE: Mutation analysis of the acetylcholine receptor (AChR) epsilon subunit gene in patients with sporadic or autosomal recessive congenital myasthenic syndromes (CMS). BACKGROUND: The nicotinic ... [more ▼]

OBJECTIVE: Mutation analysis of the acetylcholine receptor (AChR) epsilon subunit gene in patients with sporadic or autosomal recessive congenital myasthenic syndromes (CMS). BACKGROUND: The nicotinic AChR of skeletal muscle is a neurotransmitter-gated ion channel that mediates synaptic transmission at the vertebrate neuromuscular junction. Mutations in its gene may cause congenital myasthenic syndromes. A recently described mutation in exon 12 of the AChR epsilon subunit (epsilon1267delG) disrupts the cytoplasmic loop and the fourth transmembrane region (M4) of the AChR epsilon subunit. METHODS: Forty-three CMS patients from 35 nonrelated families were clinically classified as sporadic cases of CMS (group III according to European Neuromuscular Centre consensus) and were analyzed for epsilon1267delG by PCR amplification and sequence analysis. RESULTS: The authors report the complete genomic sequence and organization of the gene coding for the epsilon subunit of the human AChR (accession number AF105999). Homozygous epsilon1267delG was identified in 13 CMS patients from 11 independent families. All epsilon1267delG families were of Gypsy or southeastern European origin. Genotype analysis indicated that they derive from a common ancestor (founder) causing CMS in the southeastern European Gypsy population. Phenotype analysis revealed a uniform pattern of clinical features including bilateral ptosis and mild to moderate fatigable weakness of ocular, facial, bulbar, and limb muscles. CONCLUSIONS: The mutation epsilon1267delG might be frequent in European congenital myasthenic syndrome patients of Gypsy ethnic origin. In general, patients (epsilon1267delG) were characterized by the onset of symptoms in early infancy, the presence of ophthalmoparesis, positive response to anticholinesterase treatment, and the benign natural course of the disease. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailLe schéma de développement de l'espace régional
Delnoy, Michel ULg

in Immobilier (1999), 18

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailLa loi du 4 mai 1999 instaurant la responsabilité pénale des personnes morales: une extension du filet pénal modalisée
Masset, Adrien ULg

in Journal des Tribunaux (1999), 5940(1999), 653-660

Le droit pénal belge, après plus de 130 années de fidélité au principe de l’irresponsabilité pénale des sociétés, vient de bouleverser cette question en adoptant la loi du 4 mai 1999 instaurant la ... [more ▼]

Le droit pénal belge, après plus de 130 années de fidélité au principe de l’irresponsabilité pénale des sociétés, vient de bouleverser cette question en adoptant la loi du 4 mai 1999 instaurant la responsabilité pénale des personnes morales: les sociétés et groupements peuvent être sanctionnés de peines en cumul ou non avec les personnes physiques au travers desquelles ils ont agi. [less ▲]

Detailed reference viewed: 362 (35 ULg)
Full Text
Peer Reviewed
See detailPregnancy-associated glycoprotein levels in pregnant goats inoculated with Toxoplasma gondii or Listeria monocytogenes: a retrospective study
Zarrouk, A.; Engeland, I. V.; Sulon, Joseph ULg et al

in Theriogenology (1999), 52(6), 1095-104

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were ... [more ▼]

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were examined regularly by ultrasonography. In T. gondii-infected females (n = 5), a slow decrease of PAG was observed throughout a period of 55 to 74 d after inoculation. Afterwards, the goats either aborted (n = 4) or kidded 1 dead and 1 weak fetus (n = 1). In L. monocytogenes-infected females (n = 8), a marked decrease of PAG was observed from the day of inoculation. Abortion occurred within 9 to 11 d post inoculation (n = 7). Only 1 goat kidded a healthy fetus. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Peer Reviewed
See detailPhase segregation and avalanches in multispecies sandpiles
Vandewalle, Nicolas ULg

in Physica A: Statistical Mechanics and its Applications (1999), 272(3-4), 450-458

A stochastic lattice model for the simulation of sandpiles is presented. The model allows for the numerical investigation of the phase segregation phenomenon occurring when different granular species are ... [more ▼]

A stochastic lattice model for the simulation of sandpiles is presented. The model allows for the numerical investigation of the phase segregation phenomenon occurring when different granular species are initially mixed together before deposition. The model includes the collective motion of grains along the pile surface and produces the so-called landslides. The parameters of the model are discussed. The distribution of avalanche sizes is found to broaden when the number of grain types increases. This result raises new questions about previously reported experimental observations of avalanche size distributions. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailMedial temporal lobe metabolic impairment in dementia associated with motor neuron disease
Garraux, Gaëtan ULg; Salmon, Eric ULg; Degueldre, Christian ULg et al

in Journal of the Neurological Sciences (1999), 168(2), 145-150

In the course of their disease certain patients with frontotemporal dementia (FTD) develop clinical features compatible with a motor neuron disease (FTD-MND). Previous reports have suggested that the ... [more ▼]

In the course of their disease certain patients with frontotemporal dementia (FTD) develop clinical features compatible with a motor neuron disease (FTD-MND). Previous reports have suggested that the functional pattern is similar in FTD and FTD-MND. However, some neuropathological studies suggest greater involvement of medial temporal regions in FTD-MND than in FTD. Using statistical parametric mapping (SPM96), we compared the metabolic patterns obtained at rest with positron emission tomography in 10 FTD patients and three FTD-MND patients with those obtained from 46 healthy subjects (HS). Mean age, duration of illness and dementia stage did not differ statistically between the FTD and FTD-MND groups. In comparison with HS, both groups showed frontal and anterior temporal hypometabolism at P<0.001. When the FTD-MND group was compared to the FTD group, significant hypometabolism was only observed in bilateral amygdala, bilateral hippocampus, and bilateral enthorinal and parahippocampal regions (Brodmann's areas, BA 28/36) at P<0.005. We found no significant differences in regional glucose uptake when FTD patients were contrasted to FTD-MND patients. Our results suggest statistically comparable frontal and lateral temporal hypometabolism in both conditions but greater impairment of medial temporal lobe activity in FTD-MND. Our results and a review of the literature support the hypothesis that there is a functional continuum between classical motor neuron disease (cMND), FTD-MND, and FTD. [less ▲]

Detailed reference viewed: 28 (1 ULg)
Full Text
Peer Reviewed
See detailEffectiveness of poly(vinylpyridine) block copolymers as stabilizers of aqueous titanium dioxide dispersions of a high solid content
Creutz, Serge; Jérôme, Robert ULg

in Langmuir (1999), 15(21), 7145-7156

Diblock copolymers of 4-vinylpyridine (4VP) and sodium methacrylate (MANa) have been synthesized and tested as dispersants for aqueous dispersions (80 wt % solid) of alumina-coated titanium dioxide. P4VP ... [more ▼]

Diblock copolymers of 4-vinylpyridine (4VP) and sodium methacrylate (MANa) have been synthesized and tested as dispersants for aqueous dispersions (80 wt % solid) of alumina-coated titanium dioxide. P4VP is strongly anchored onto the pigment surface. The molecular composition of the block copolymers has an effect on the amount of copolymer required for imparting good stability to the slurry. Where the two parent triblock copolymers are concerned, triblocks consisting of PMANa outer blocks have a stabilizing efficiency comparable to the diblock copolymers, whereas the reverse structure is much less efficient. In the case of a nonblock distribution of 4VP and MANa in the copolymer chains, the stabilization effect is lost. Finally, the comparison of different stabilizing blocks attached to the same anchoring block confirms that the electrosteric stabilization mechanism is superior to the steric stabilization mechanism. A decrease in the anchoring strength of the diblocks has a deleterious effect on the dispersion stability. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailOxidation Sensitivity May Be a Useful Tool for the Detection of the Hematotoxic Potential of Newly Developed Molecules: Application to Antipsychotic Drugs
Liégeois, Jean-François ULg; Bruhwyler, J.; Petit, C. et al

in Archives of Biochemistry & Biophysics (1999), 370(1), 126-37

Some antipsychotic agents have been found to produce agranulocytosis and aplastic anemia. The oxidation phenomena and/or the formation of free radicals has been suggested to be causally related to various ... [more ▼]

Some antipsychotic agents have been found to produce agranulocytosis and aplastic anemia. The oxidation phenomena and/or the formation of free radicals has been suggested to be causally related to various hematological disorders, e.g., agranulocytosis. Using five experimental conditions, we tested the oxidative potential of compounds with and without a history of hematological side effects, e.g., agranulocytosis and aplastic anemia. A statistical analysis was undertaken for each experimental condition and a multivariate analysis combining all results was performed. Two peroxidase-induced free radical models did not successfully discriminate between drugs with and without a history of causing hematologic problems (<70%). The lipid peroxidation system provided even less satisfactory discrimination, with only 56.25% correct classification. However, an 87.5% correct classification was obtained when using the oxidation potentials of these drugs determined at pH 4.7 and at pH 7.4. A multivariate analysis taking into account the five variables provided 87.5% success in classification. The two clusters were better discriminated in terms of a "distance coefficient." In a second analysis, the putative antipsychotic pyridobenzodiazepine analogues (JL5, JL8, JL18, and JL25) were classified in the cluster of toxic compounds, while the oxa- and thiazepine analogues (JL2, JL3, and JL13) were classified as nontoxic compounds. On the other hand, a few metabolites of clozapine and fluperlapine were classified in the toxic compound group. The procedure described herein is, to our knowledge, the first which classifies molecules of different structures as well as different pharmacological profiles according to their hematotoxic potential. Such a procedure could be used to predict drug-induced hematological side effects. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailOn some stability properties of the discretization of damped propagation of shallow-water inertia–gravity waves on the Arakawa B-grid
Beckers, Jean-Marie ULg

in Ocean Modelling (1999), 1(2-4), 53-69

We investigate a problem mentioned by Deleersnijder and Campin (Deleersnijder, E., & Campin, J. -M. (1993). Ocean Modelling 97, 2), who looked at the problem of the discretization of inertia–gravity waves ... [more ▼]

We investigate a problem mentioned by Deleersnijder and Campin (Deleersnijder, E., & Campin, J. -M. (1993). Ocean Modelling 97, 2), who looked at the problem of the discretization of inertia–gravity waves in the staggered B-grid. We generalize this study by adding diffusion. General stability conditions are found with the help of Miller's theorem, and the paradox found in Deleersnijder and Campin (Deleersnijder, E., & Campin, J. -M. (1993). Ocean Modelling 97, 2) is discussed. It is argued that it stems from inappropriate application of boundary conditions in conjunction with a Coriolis force treatment which could produce mechanical work. [less ▲]

Detailed reference viewed: 14 (4 ULg)
See detailEffets antitumoraux des agonistes dopaminergiques et des analogues de la somatostatine
Beckers, Albert ULg; Valdes Socin, Hernan Gonzalo ULg; Stevenaert, Achille ULg

in Médecine Thérapeutique Endocrinologie (1999), 1(2), 183-191

Le traitement médicamenteux des adénomes hypophysaires commence son histoire au début des années 70. La bromocriptine qui vient d’être découverte s’avère efficace pour traiter l’hyperprolactinémie et ... [more ▼]

Le traitement médicamenteux des adénomes hypophysaires commence son histoire au début des années 70. La bromocriptine qui vient d’être découverte s’avère efficace pour traiter l’hyperprolactinémie et restaurer la fertilité chez les femmes hyperprolactinémiques. Son effet antitumoral est découvert un peu plus tard lorsque des scanners performants permettent d’observer une diminution de volume de l’adénome. Au cours des vingt dernières années, des progrès substantiels ont été accomplis dans le traitement médical des adénomes hypophysaires et dans les techniques qui permettent leur observation et leur suivi. Des molécules plus puissantes que la bromocriptine sont maintenant disponibles pour le traitement des adénomes à prolactine (PRL). Les plus utilisées et les plus connues sont le quinagolide et la cabergoline. En outre, de nouveaux traitements médicamenteux, s’adressant cette fois à l’adénome somatotrope, ont vu le jour. La somatostatine a été découverte en 1973. Sa demi-vie très courte et les effets rebond observés après son injection ne permettaient toutefois pas de l’utiliser pour soigner l’acromégalie. Il a fallu attendre le milieu des années 80 pour disposer du premier analogue efficace dans cette indication : l’octréotide. Depuis, les préparations à effet retard ont vu le jour (Sandostatine® LAR, Somatuline® LP). Elles permettent une normalisation de la sécrétion de l’hormone de croissance (growth hormone, GH) dans l’acromégalie dans plus de la moitié des cas. Il est rapidement devenu évident que ces médicaments étaient aussi capables d’entraîner une réduction du volume des adénomes dans un pourcentage significatif de cas. Parallèlement, les progrès techniques ont permis la mise au point de scanners hyperperformants et, surtout, d’examens par résonance magnétique nucléaire (IRM). Actuellement, ce type d’examens a remplacé le scanner dans l’évaluation des adénomes hypophysaires. Il permet, en effet, de repérer des lésions de plus petite taille qu’avant, de mieux définir les limites entre la tumeur, l’hypophyse normale et les structures avoisinantes, notamment dans les macro-adénomes, et, en outre, de mieux caractériser les phénomènes intratumoraux tels que la nécrose ou l’hémorragie. Dans cet article nous revoyons les effets antitumoraux des analogues de la somatostatine et des agonistes dopaminergiques dans l’acromégalie et chez les patients porteurs d’adénome à prolactine. Les divers types de réponses, aussi bien dans les micro-adénomes que dans les macro-adénomes, seront envisagés à l’occasion de la description de cas cliniques illustratifs, puis une synthèse générale sera présentée. [less ▲]

Detailed reference viewed: 177 (0 ULg)
Full Text
Peer Reviewed
See detailThe Catalytic, Glycosyl Transferase and Acyl Transferase Modules of the Cell Wall Peptidoglycan-Polymerizing Penicillin-Binding Protein 1b of Escherichia Coli
Terrak, Mohammed ULg; Ghosh, Tushar K.; van Heijenoort, Jean et al

in Molecular Microbiology (1999), 34(2), 350-364

The penicillin-binding protein (PBP) 1b of Escherichia coli catalyses the assembly of lipid-transported N-acetyl glucosaminyl-beta-1, 4-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelyl ... [more ▼]

The penicillin-binding protein (PBP) 1b of Escherichia coli catalyses the assembly of lipid-transported N-acetyl glucosaminyl-beta-1, 4-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelyl+ ++- (L)-D-alanyl-D-alanine disaccharide pentapeptide units into polymeric peptidoglycan. These units are phosphodiester linked, at C1 of muramic acid, to a C55 undecaprenyl carrier. PBP1b has been purified in the form of His tag (M46-N844) PBP1bgamma. This derivative provides the host cell in which it is produced with a functional wall peptidoglycan. His tag (M46-N844) PBP1bgamma possesses an amino-terminal hydrophobic segment, which serves as transmembrane spanner of the native PBP. This segment is linked, via an congruent with 100-amino-acid insert, to a D198-G435 glycosyl transferase module that possesses the five motifs characteristic of the PBPs of class A. In in vitro assays, the glycosyl transferase of the PBP catalyses the synthesis of linear glycan chains from the lipid carrier with an efficiency of congruent with 39 000 M-1 s-1. Glu-233, of motif 1, is central to the catalysed reaction. It is proposed that the Glu-233 gamma-COOH donates its proton to the oxygen atom of the scissile phosphoester bond of the lipid carrier, leading to the formation of an oxocarbonium cation, which then undergoes attack by the 4-OH group of a nucleophile N-acetylglucosamine. Asp-234 of motif 1 or Glu-290 of motif 3 could be involved in the stabilization of the oxocarbonium cation and the activation of the 4-OH group of the N-acetylglucosamine. In turn, Tyr-310 of motif 4 is an important component of the amino acid sequence-folding information. The glycosyl transferase module of PBP1b, the lysozymes and the lytic transglycosylase Slt70 have much the same catalytic machinery. They might be members of the same superfamily. The glycosyl transferase module is linked, via a short junction site, to the amino end of a Q447-N844 acyl transferase module, which possesses the catalytic centre-defining motifs of the penicilloyl serine transferases superfamily. In in vitro assays with the lipid precursor and in the presence of penicillin at concentrations sufficient to derivatize the active-site serine 510 of the acyl transferase, the rate of glycan chain synthesis is unmodified, showing that the functioning of the glycosyl transferase is acyl transferase independent. In the absence of penicillin, the products of the Ser-510-assisted double-proton shuttle are glycan strands substituted by cross-linked tetrapeptide-pentapeptide and tetrapeptide-tetrapeptide dimers and uncross-linked pentapeptide and tetrapeptide monomers. The acyl transferase of the PBP also catalyses aminolysis and hydrolysis of properly structured thiolesters, but it lacks activity on D-alanyl-D-alanine-terminated peptides. This substrate specificity suggests that carbonyl donor activity requires the attachment of the pentapeptides to the glycan chains made by the glycosyl transferase, and it implies that one and the same PBP molecule catalyses transglycosylation and peptide cross-linking in a sequential manner. Attempts to produce truncated forms of the PBP lead to the conclusion that the multimodular polypeptide chain behaves as an integrated folding entity during PBP1b biogenesis. [less ▲]

Detailed reference viewed: 62 (17 ULg)
Full Text
Peer Reviewed
See detailNew catalysis for fast bulk ring-opening polymerization of lactide monomers
Degée, Philippe; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecular Symposia (1999), 144

The ring-opening polymerization of lactides has been studied in bulk using either 2-ethylhexanoic acid tin(II) salt, Sn(Oct)2, or aluminum triisopropoxide, Al(OiPr)3, as the initiator over a wide range of ... [more ▼]

The ring-opening polymerization of lactides has been studied in bulk using either 2-ethylhexanoic acid tin(II) salt, Sn(Oct)2, or aluminum triisopropoxide, Al(OiPr)3, as the initiator over a wide range of temperature and monomer-to-initiator molar ratio, A high increase in the bulk polymerization rate has been observed when the initiator was added with an equimolar amount of a Lewis base, such as triphenylphosphine and 4-picoline (C6H7N) added to Sn(Oct)2 and Al(OiPr)3, respectively Melt stable polylactides of high molecular weight and reasonably narrow molecular weight distribution have been accordingly prepared. The use of the Sn(Oct)2. equimolar combination has allowed for reaching an acceptable balance between propagation and depolymerization rates, so that the polymerization is fast enough to be performed through a continuous single-stage process in a twin-screw extruder A global activation mechanism is proposed and discussed by comparison with both investigated initiation systems. [less ▲]

Detailed reference viewed: 93 (15 ULg)
Full Text
Peer Reviewed
See detailA general framework for power system transient stability control
Pavella, Mania ULg; Ernst, Damien ULg; Ruiz-Vega, Daniel

in IEEE Power Engineering Society Letters (1999), 19(10), 45-46

Detailed reference viewed: 47 (6 ULg)
Peer Reviewed
See detailReflux gastro-oesophagien et asthme
Lamproye, Anne ULg; Louis, Edouard ULg; Louis, Renaud ULg et al

in Revue Médicale de Liège (1999), 54(10), 805-8

Gastro-oesophageal reflux (GER) is more frequent in asthmatic patients than in the community at large. A causative association between the two diseases is suspected. Twenty-four hour ambulatory ... [more ▼]

Gastro-oesophageal reflux (GER) is more frequent in asthmatic patients than in the community at large. A causative association between the two diseases is suspected. Twenty-four hour ambulatory intraoesophageal pH monitoring represents the golden standard for the diagnosis of GER. The medical and/or surgical treatment of reflux in asthmatic patients with GER can improve pulmonary symptoms and to a lesser extent pulmonary function. The selection of the patients who will benefit from a GER treatment is difficult. Some symptoms like intrinsic asthma, nocturnal crises, could predict a good response to GER treatment. [less ▲]

Detailed reference viewed: 44 (6 ULg)
Full Text
Peer Reviewed
See detailL'enfant dans l'Égypte gréco-romaine
Marganne, Marie-Hélène ULg

in Revue de Pédiatrie (La) (1999), 30(298-7), 30-37

Detailed reference viewed: 23 (5 ULg)
Full Text
Peer Reviewed
See detailAlphacalcidol in Prevention of Glucocorticoid-Induced Osteoporosis
Reginster, Jean-Yves ULg; de Froidmont, C.; Lecart, M. P. et al

in Calcified Tissue International (1999), 65(4), 328-31

One of the major drawbacks of glucocorticoids long-term therapy is the occurrence of a severe osteoporosis characterized by fractures occurring at different sites, mainly at the level of trabecular bone ... [more ▼]

One of the major drawbacks of glucocorticoids long-term therapy is the occurrence of a severe osteoporosis characterized by fractures occurring at different sites, mainly at the level of trabecular bone. One of the major determinants of glucocorticoid-induced osteoporosis is a decrease in the intestinal absorption of calcium (Ca) leading to a secondary hyperparathyroidism. D-hormones have been shown to significantly improve Ca absorption in the gut and subsequently to decrease parathyroid hormone circulating levels, hence normalizing bone turnover. In a recent study evaluating 145 patients suffering from diseases requiring long-term treatment with high doses of corticosteroids, we have demonstrated a significant benefit of alphacalcidol (1 microg/day) over placebo in terms of changes in bone mineral density of the lumbar spine. These results are in accordance with studies showing better prevention of bone loss and vertebral fractures in cardiac transplant patients treated with alphacalcidol than those treated with etidronate. There is now a convergent body of evidence to suggest that alphacalcidol is a reasonable, safe, and effective option for the prevention of glucocorticoid-induced osteoporosis, provided that serum Ca is monitored on a regular basis. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailPharma clinics. Le médicament du mois. La lercanidipine (Zanidip)
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1999), 54(10), 832-834

Lercanidipine (Zanidip) is a new calcium antagonist belonging to the dihydropyridine family suitable for the first-line treatment of hypertension. This molecule displays a high specificity and selectivity ... [more ▼]

Lercanidipine (Zanidip) is a new calcium antagonist belonging to the dihydropyridine family suitable for the first-line treatment of hypertension. This molecule displays a high specificity and selectivity for vascular smooth muscle cells and has, in spite of a short plasma half-life, a long duration of action due to its liposolubility. The usual and once a day dose to treat high blood pressure of any grade is 10 mg, which if needed could be increased to 20 mg once a day. It has a gradual onset of effect on blood pressure, thus leading to a good tolerability and hopefully a better compliance in the treatment of hypertension. No adaptation of the dose is needed in older patients or in patients with moderate renal or liver impairment. [less ▲]

Detailed reference viewed: 279 (0 ULg)
Full Text
Peer Reviewed
See detailAssociation of Tibolone and Fluoride Displays a Pronounced Effect on Bone Mineral Density in Postmenopausal Osteoporotic Women
Reginster, Jean-Yves ULg; Agnusdei, D.; Gennari, C. et al

in Gynecological Endocrinology : The Official Journal of the International Society of Gynecological Endocrinology (1999), 13(5), 361-8

A double-blind, placebo-controlled, randomized, prospective two-center study was carried out to assess the effects of tibolone + fluoride versus placebo + fluoride therapy on trabecular and cortical bone ... [more ▼]

A double-blind, placebo-controlled, randomized, prospective two-center study was carried out to assess the effects of tibolone + fluoride versus placebo + fluoride therapy on trabecular and cortical bone in postmenopausal osteoporotic women. Ninety-four subjects (mean age 61.1 years, postmenopausal 13.5 years on average) with low bone mineral density (BMD) at baseline were randomized to 2.5 mg of tibolone (Org OD 14, Livial) plus 26.4 mg of fluoride (Fluocalcic) or placebo plus 26.4 mg of fluoride daily over 2 years; 55 (58.5%) subjects completed the study, the main reason for discontinuation being untoward gastrointestinal effects. BMD at the lumbar spine was measured by both dual photon absorptiometry (DPA) and dual-energy X-ray absorptiometry (DXA), and at the hip by DXA at 6-month intervals. Baseline values (DXA, g/cm2) for tibolone + fluoride and placebo + fluoride groups were 0.733 and 0.744 for the lumbar spine, and 0.761 and 0.788 for the hip. Change from baseline and percentage change from baseline were calculated for the intent-to-treat and completers groups. An analysis of variance (ANOVA) model or Wilcoxon test was used for statistical evaluation. There was a mean increase in BMD at the lumbar spine measured by DPA of 25.3% and 12.3% in tibolone + fluoride and placebo + fluoride groups, respectively (p = 0.01); with DXA, respective changes were 32.6% and 14.0% (p = 0.013). Data on BMD at the hip showed mean increases of 7.9% and 2.6% for the tibolone + fluoride and placebo + fluoride groups, respectively. We conclude that combined tibolone + fluoride treatment induces a highly significant increase in BMD at the lumbar spine without simultaneous loss of the cortical bone allowing for a meaningful reduction of the fluoride dose when given in combination with tibolone. [less ▲]

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailLack of Cell Wall Peptidoglycan Versus Penicillin Sensitivity: New Insights into the Chlamydial Anomaly
Ghuysen, Jean-Marie ULg; Goffin, Colette

in Antimicrobial Agents and Chemotherapy (1999), 43(10), 2339-2344

Detailed reference viewed: 48 (4 ULg)
Full Text
Peer Reviewed
See detailLa chirurgie des yeux dans les papyrus grecs d’Égypte
Marganne, Marie-Hélène ULg

in Réalités Ophtalmologiques (1999), 68

Detailed reference viewed: 40 (1 ULg)
Full Text
Peer Reviewed
See detailShemin Pathway and Peroxidase Deficiency in a Fully Habituated and Fully Heterotrophic Non-Organogenic Sugarbeet Callus: An Adaptative Strategy or the Consequence of Modified Hormonal Balances and Sensitivities in These Cancerous Cells? A Review an
Gaspar, Thomas ULg; Kevers, Claire ULg; Bisbis, Badia et al

in Cell Proliferation (1999), 32(5), 249-70

There are many arguments for considering a specific fully habituated (auxin and cytokinin-independent) and fully heterotrophic non-organogenic (HNO) sugarbeet callus cell line as terminating a neoplastic ... [more ▼]

There are many arguments for considering a specific fully habituated (auxin and cytokinin-independent) and fully heterotrophic non-organogenic (HNO) sugarbeet callus cell line as terminating a neoplastic progression, and thus to be made of cancerous cells. The similarities with animal tumour and cancer cells are recalled. All types of habituated tissues examined in the literature share at least three common biochemical characteristics: low apparent peroxidase activity, high content of polyamines (PAs) and low production of ethylene. However, results concerning their auxin and cytokinin levels are not consistent. Peroxidase synthesis in the achlorophyllous HNO callus appears to arise from aminolevulinic acid (ALA) synthesis through the Shemin pathway, commonly used by animals and fungi. This pathway is limited by disturbed nitrogen metabolism that diverts glutamate (directly used for ALA synthesis in green higher plants) from the Kreb's cycle into PA synthesis. There is no argument to suggest that the low ethylene production is caused by a competition with PAs for their common precursor, S-adenosylmethionine. The results we report here indicate modified anabolic and catabolic pathways of auxins and cytokinins but also the possibilities of unusual compounds playing similar roles (dehydrodiconiferyl alcohol glucosides, for instance). A higher turnover of PAs is shown in the HNO callus, which could suggest a role for H2O2 and gamma-aminobutyric acid, products or intermediates in the PA catabolic pathway, as secondary messengers. The habituated cells retain some sensitivity towards exogenous auxins and cytokinins. Their increased sensitivity to PAs and ethylene suggests modified hormonal balances for the control of these actively dividing cells. [less ▲]

Detailed reference viewed: 56 (0 ULg)
Peer Reviewed
See detailCrossover Iliofemoral Bypass Grafting for Treatment of Unilateral Iliac Atherosclerotic Disease
Defraigne, Jean-Olivier ULg; Vazquez, C.; Limet, Raymond ULg

in Journal of Vascular Surgery : Official Publication, the Society for Vascular Surgery and International Society For Cardiovascular Surgery, North American Chapter (1999), 30(4), 693-700

PURPOSE: In patients with unilateral iliac disease, a less invasive procedure than aortobifemoral bypass grafting may be desirable, especially in poor-risk patients or when sexual dysfunction is feared ... [more ▼]

PURPOSE: In patients with unilateral iliac disease, a less invasive procedure than aortobifemoral bypass grafting may be desirable, especially in poor-risk patients or when sexual dysfunction is feared. In these cases, femorofemoral (FF) bypass grafting is often proposed. Compared with FF bypass grafting, iliofemoral (IF) bypass grafting avoids bilateral exposure of the groins, which may reduce the risk of infection. When the primitive iliac artery is occluded from its origin or heavily calcified, one may use the contralateral artery as inflow, after a small retroperitoneal exposure, to perform a crossover iliofemoral (CIF) bypass grafting procedure, through the Retzius space. Our 10-year experience with CIF bypass grafting in a select group of patients was studied. METHODS: Between 1986 and 1996, 36 patients underwent CIF bypass grafting for symptomatic unilateral iliac occlusion or stenosis. All patients were examined by means of Doppler ultrasound scanning and underwent bilateral multiplane angiography. Patients were considered for this procedure when the ipsilateral common iliac artery was occluded from its origin or was diffusely and heavily calcified. The decision to perform a CIF bypass grafting procedure was made when no significant disease of the contralateral common iliac artery was seen, and patients who had features of contralateral iliac disease were excluded. The main outcomes were perioperative mortality and morbidity, long-term primary and secondary patency rates, and limb salvage rate. RESULTS: The study included 31 men and five women, with a mean age of 58.8 years. Indications for bypass grafting were disabling claudication (26 of 36 patients, 72%) and limb-threatening ischemia (10 of 26 patients, 28%). Twelve procedures were performed simultaneously: endarterectomy of the recipient common femoral artery (n = 3), femoropopliteal bypass grafting (n = 4, 11.1%), profundoplasty (n = 4, 11%), and right internal carotid endarterectomy (n = 1). New postoperative erectile dysfunction did not develop in any of the patients. The survival rate was 97.3% at 1 year and 68.5% at 5 years. The primary and secondary patency rates were 94% and 100%, respectively, at 1 year and 76.7% and 95%, respectively, at 5 years. The limb salvage rate was 100% at 1 year and 87% at 3 years. CONCLUSION: The operative mortality associated with CIF is low. The long-term primary and secondary patency rates are satisfactory, and they are lower than those reported for aortobifemoral bypass grafting. This procedure does not preclude a later performance of an aortobifemoral bypass grafting procedure. CIF bypass grafting is not only suitable for poor-risk patients with a limited life expectancy who have the appropriate arterial anatomy, but also may be warranted for young patients in whom erectile dysfunction is feared. [less ▲]

Detailed reference viewed: 30 (0 ULg)
Peer Reviewed
See detailRole of Nuclear Factor-Kappa B in Colon Cancer Cell Apoptosis Mediated by Aminopyropheophorbide Photosensitization
Matroule, J. Y.; Hellin, A. C.; Morliere, P. et al

in Photochemistry & Photobiology (1999), 70(4), 540-8

Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells ... [more ▼]

Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photosensitization was cytotoxic for HCT-116 cells through an induction of apoptosis. Indeed, DNA fragmentation (DNA laddering and terminal deoxyuridine nick-end labeling) and chromatin condensation (4',6-diamidine-2'-phenylindole staining) could be visualized soon after photosensitization. Because nuclear factor (NF)-kappa B is involved in the response to many photosensitizers, we also demonstrated its nuclear translocation in two waves: a rapid and transient one, followed by a slow and sustained phase. The NF-kappa B turned out to be involved in an antiapoptotic response to APP-mediated photosensitization because the HCT-116 cell line expressing the dominant negative mutant of inhibitor-kappa B alpha was more sensitive to apoptosis as measured by DNA fragmentation and caspase activation. These data unambiguously show that a membrane-located photosensitizer can lead to effective apoptosis, reinforcing the idea that PDT can be an effective means to eradicate colon cancer cells. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailCulture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials in Vitro
Simain-Sato, Franklin ULg; Lahmouzi, Jamila ULg; Kalykakis, G. K. et al

in Journal of Periodontology (1999), 70(10), 1234-9

BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts ... [more ▼]

BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery. METHODS: Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours. RESULTS: When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used. CONCLUSIONS: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR. [less ▲]

Detailed reference viewed: 80 (2 ULg)
Full Text
Peer Reviewed
See detailLateral structures of thin films of ampholytic diblock copolymers adsorbed from dilute aqueous solution at the solid/liquid Interface
Walter, H.; Muller-Buschbaum, P.; Gutmann, J. S. et al

in Langmuir (1999), 15(20), 6984-6990

The lateral structures of dried thin films of the ampholytic diblock copolymer poly((methacrylic acid)-block-((dimethylamino)ethyl methacrylate)) adsorbed from dilute aqueous solution onto silicon ... [more ▼]

The lateral structures of dried thin films of the ampholytic diblock copolymer poly((methacrylic acid)-block-((dimethylamino)ethyl methacrylate)) adsorbed from dilute aqueous solution onto silicon substrates were investigated by scanning force microscopy (SFM) and diffuse X-ray scattering. The adsorbed amount of polymer, as a function of pH, reveals a maximum near the isoelectric point (IEP) of the polyampholyte. Different lateral structures are determined for samples adsorbed at pH values above the maximum of the adsorbed amount, as compared to the ones adsorbed at pH values below this maximum. At a pH around the IEP, the polyampholyte precipitates and the layers are formed by adsorption of flocks with sizes in the micrometer range. Lateral structures measured by SFM are similarly detected by diffuse X-ray scattering. The later technique delivers a statistical description of the lateral surface structures averaged over a larger area. The structures investigated are related to the polyampholyte structure in solution and can be explained by the interplay of the electrostatic interactions of the two oppositely charged blocks of the polyampholyte and the charged surface, as well as by the dependencies of charge densities on pH. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailBiological properties of human prolactin analogs depend not only on global hormone affinity, but also on the relative affinities of both receptor binding sites
Kinet, Sandrina; Bernichtein, Sophie; Kelly, Paul A. et al

in Journal of Biological Chemistry (1999), 274(37), 26033-43

Zinc increases the affinity of human growth hormone (hGH) for the human prolactin receptor (hPRLR) due to the coordination of one zinc ion involving Glu-174(hGH) and His-18(hGH). In contrast, binding of ... [more ▼]

Zinc increases the affinity of human growth hormone (hGH) for the human prolactin receptor (hPRLR) due to the coordination of one zinc ion involving Glu-174(hGH) and His-18(hGH). In contrast, binding of hPRL to the hPRLR is zinc-independent. We engineered in binding site 1 of hPRL a hGH-like zinc coordination site, by mutating Asp-183(hPRL) (homologous to Glu-174(hGH)) into Glu (D183E mutation). This mutation was also introduced into G129R hPRL, a binding site 2 mutant (Goffin, V., Kinet, S., Ferrag, F., Binart, N., Martial, J. A. , and Kelly, P. A. (1996) J. Biol. Chem. 271, 16573-16579). These analogs were characterized using a stable clone expressing both the hPRLR and a PRLR-responsive reporter gene. The D183E mutation per se decreases the binding affinity and transcriptional activity of hPRL. However, this loss is partially rescued by the addition of zinc and the effect is much more marked on bioactivity than on binding affinity. These data indicate that the D183E mutation confers zinc sensitivity to hPRL biological properties. Due to an impaired site 2, the agonistic activity of G129R analog is almost nil. Although the double mutant D183E/G129R displays lower affinity ( approximately 1 log) compared with G129R hPRL, it unexpectedly recovers partial agonistic activity in the absence of zinc. Moreover, whereas zinc increases the affinity of D183E/G129R, it paradoxically abolishes its agonistic activity. Our results demonstrate that the biological properties of hPRL analogs do not necessarily parallel their overall affinity. Rather, the relative affinities of the individual binding sites 1 and 2 may play an even more important role. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailEstrogen Receptor-Beta in Quail: Cloning, Tissue Expression and Neuroanatomical Distribution
Foidart, Agnès ULg; Lakaye, Bernard ULg; Grisar, Thierry ULg et al

in Journal of Neurobiology (1999), 40(3), 327-42

A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA ... [more ▼]

A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA sequence of the quail ERbeta that contains a complete open reading frame. For the first time in an avian species, this cDNA sequence and the corresponding amino acid sequence are described. They are compared with the known ERbeta sequences previously described in mammals and with the ERalpha sequences identified in a selection of mammalian and avian species. The analysis by Northern blotting of the ERbeta mRNA expression in the brain and kidneys revealed the presence of several transcripts. The presence of ERbeta identified by reverse transcriptase-polymerase chain reaction demonstrated a widespread distribution quite different from the distribution of ERalpha. The complete neuroanatomical distribution of ERbeta mRNA as determined by in situ hybridization with 35S- and 33P-labeled oligoprobes is also presented. Transcripts are present in many nuclei implicated in the control of reproduction such as the medial preoptic nucleus, the nucleus striae terminalis, and the nucleus taeniae, the avian homologue of the amygdala. These data demonstrate the presence of ERbeta in a nonmammalian species and indicate that the (neuro)-anatomical distribution of this receptor type has been conserved in these two classes of vertebrates. The role of this receptor in the control of reproduction and other physiological processes should now be investigated. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Peer Reviewed
See detailComparison of Eae, Tir, Esp a and Esp b Genes of Bovine and Human Attaching and Effacing Escherichia Coli by Multiplex Polymerase Chain Reaction
China, B.; Goffaux, F.; Pirson, V. et al

in FEMS Microbiology Letters (1999), 178(1), 177-82

Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments ... [more ▼]

Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
See detailDeconvolving spectra of lensing galaxies, QSO hosts, and more ...
Courbin, F.; Magain, Pierre ULg; Sohy, Sandrine ULg et al

in The Messenger (1999), 97

Detailed reference viewed: 3 (0 ULg)