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See detailIGF-II in primary human colorectal tumors: peptide level, activated promoters, parental imprinting and gene rearrangement.
Winkler, Rose ULg; Delacroix, Laurence ULg; Bensbaho, K. et al

in Hormone & Metabolic Research (1999), 31(2-3), 148-54

IGF-II is a polypeptide growth factor with growth and differentiation promoting activities, involved in human development. We have reported previously IGF-II mRNA and peptide overexpression in primary ... [more ▼]

IGF-II is a polypeptide growth factor with growth and differentiation promoting activities, involved in human development. We have reported previously IGF-II mRNA and peptide overexpression in primary human colon cancers. Here we show that the IGF-II peptide content is increased in six primary colon cancers compared to the corresponding healthy tissues. The IGF-II transcripts in healthy and cancerous colon tissues were identified by Northern blotting and RT-PCR. Promoters P3 and P4 were active in most tissues. Relaxation of parental imprinting was observed in two tumors and one healthy tissue, without any correlation with the IGF-II transcript levels. Rearrangements of the IGF-II gene in two tumors containing very high amounts of IGF-II mRNA are described. Fragments containing the breakpoints were cloned by the vectorette-PCR strategy. In both tumors, the breakpoints occurred in repetitive sequences. In one tumor (T11), the breakpoint was localized 2 kb downstream the end of exon 9. The second tumor (T18) contains two modified alleles. In one rearranged allele the breakpoint is located in exon 9. The exact position of the breakpoint in the second rearranged allele has not been identified. In future experiments, the correlation between the gene rearrangements and IGF-II mRNA overexpression will be studied. [less ▲]

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See detailHousekeeping Genes as Internal Standards: Use and Limits
Thellin, Olivier ULg; Zorzi, Willy ULg; Lakaye, Bernard ULg et al

in Journal of Biotechnology (1999), 75(2-3), 291-5

Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their ... [more ▼]

Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phsophoribosyltransferase (HRPT), L32. 28S, and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferred types of internal standards are then proposed for each of these samples and thereafter the general procedure concerning the choice of an internal standard and the way to manage its uses are discussed. [less ▲]

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See detailContribution de l'hypertension arterielle au risque vasculaire du diabetique.
Marre, M.; Bouhanick, B.; Hadjadj, S. et al

in Diabètes & Métabolism (1999), 25 Suppl 3

Hypertension is a major cardiovascular risk factor in diabetic subjects. Recent trials have suggested that blood pressure objectives should be < or = 140/80 mmHg. However, there is currently no evidence ... [more ▼]

Hypertension is a major cardiovascular risk factor in diabetic subjects. Recent trials have suggested that blood pressure objectives should be < or = 140/80 mmHg. However, there is currently no evidence supporting any particular preferential drug strategy for this treatment objective. [less ▲]

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See detailIntracellular visualization of BrdU-labeled plasmid DNA/cationic liposome complexes.
El Ouahabi, A.; Thiry, Marc ULg; Schiffmann, S. et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1999), 47(9), 1159-66

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA ... [more ▼]

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA complexed with a cationic lipid, Vectamidine (3-tetradecylamino-N-tert-butyl-N'-tetradecylpropionamidine) in BHK21 cells. To facilitate its detection inside the cells, bromodeoxyuridine (BrdU) was incorporated into plasmid DNA under conditions that minimize plasmid alteration. BrdU was localized in cells incubated with Vectamidine/BrdU-labeled plasmid DNA complexes by immunogold labeling and electron microscopy (EM). Labeling was predominantly associated with aggregated liposome structures at the surface of and inside the cells. EM observations of cells transfected with Vectamidine/DNA complexes showed that the liposome/DNA aggregates accumulate in large vesicles in the cell cytosol. On the other hand, using rhodamine-labeled Vectamidine and revealing BrdU with FITC-conjugated antibodies permitted simultaneous detection in the cells of both components of the complexes with confocal laser scanning microscopy. The DNA and lipids co-localized at the surface of and inside the cells, indicating that the complex is internalized as a whole. Our results show that the BrdU-labeled plasmid DNA detection system can be a useful tool to visualize exogenous DNA entry into cells by a combination of electron and confocal microscopy. [less ▲]

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See detailChanges in mediobasal hypothalamic gonadotropin-releasing hormone messenger ribonucleic acid levels induced by mating or ovariectomy in a reflex ovulator, the ferret.
Bakker, Julie ULg; Rubin, B. S.; Baum, M. J.

in Endocrinology (1999), 140(2), 595-602

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large ... [more ▼]

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large release of GnRH from the mediobasal hypothalamus (MBH). In Exp 1 we asked whether GnRH messenger RNA (mRNA) levels increase in response to mating so as to replenish the MBH GnRH stores needed to sustain the preovulatory LH surge. Estrous females were killed 0, 0.25, 0.5, 1, 3, 6, 14, or 24 h after the onset of a 10-min intromission from a male. Coronal brain sections ranging from the rostral preoptic area caudally to the posterior hypothalamus were processed for in situ hybridization using a 35S-labeled oligoprobe complementary to the human GnRH-coding region. We found no evidence of increased MBH GnRH mRNA levels during the ferret's mating-induced preovulatory LH surge. Instead, the number of GnRH mRNA-expressing cells dropped significantly in the arcuate region beginning 6 h after onset of intromission and remained low thereafter. Furthermore, cellular GnRH mRNA levels decreased in the arcuate region toward the end of the preovulatory LH surge. In Exp 2 we asked whether ovarian hormones regulate MBH GnRH mRNA levels in the female ferret. Ovariectomy of estrous females significantly reduced the number of GnRH mRNA-expressing cells in the arcuate region. This decrease was probably not due to the absence of circulating estradiol. Gonadally intact anestrous females had levels of MBH GnRH mRNA similar to those in estrous females even though plasma estradiol levels were equally low in anestrous females and ovariectomized females. Ovarian hormones other than estradiol may stimulate MBH GnRH mRNA levels in anestrous and estrous females. [less ▲]

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See detail4-Ethyl-2,3-dihydro-4H-pyrido[3,2-e]-1,2,4-thiadiazine 1,1-dioxide and 4-ethyl-2,3-dihydro-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide
Dupont, L.; Pirotte, Bernard ULg; Podona, T. et al

in Acta Crystallographica (1999), C55

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See detailNew Therapeutic options
Reginster, Jean-Yves ULg; Henrotin, Yves ULg

in Bone Depeche (1999), 5

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See detailComparison of performance, water intake and feeding behaviour of weaned pigs fed either pellets or meal
Laitat, Martine ULg; Vandenheede, Marc ULg; Desiron, Alain et al

in Animal Science (1999), 69

Performance, water intake and feeding behaviour of two groups of 30 (trial 1), 40 (trial 2) or 50 (trial 3) weaned pigs offered either pellets ol meal of the same formulation were compared. Average daily ... [more ▼]

Performance, water intake and feeding behaviour of two groups of 30 (trial 1), 40 (trial 2) or 50 (trial 3) weaned pigs offered either pellets ol meal of the same formulation were compared. Average daily weight gains (ADG) were higher for pigs given pellets rather than meal in trials 2 (413 v. 363 g/day P < 0.001) and 3 (356 v. 324 g/day, P < 0.05). Mean daily water intake (DWI) was higher with meal than with pellets but only during trial 1 (2.31 y. 1.65 l/day, P < 0.01). The occupation time (OT) and the number of animals using the feeder simultaneously (N) were higher when pigs were given meal rather than pellets, whatever the animal density: trial 1: 82.6 v. 69.9% (P = 0.05) and 3.8 v. 2.3 (P < 0.01); trial 2: 90.9 v. 77.9% (P > 0.05) and 5.2 v. 3.1 (P < 0.01); trial 3: 96.2 v. 83.6% (P < 0.05) and 5.9 v. 3.8 (P < 0.01). When using pellets, OT and N were always significantly lower during the night than during the day but when using meal in groups of 40 and 50, OT during the night was almost as high as during the day. The greater the group size, the lower were ADG (both diets) and DWI (only with meal) and the higher were OT and N (both diets). Furthermore, significant linens and curvilinear regressions of DWI, OT and N according to time were calculated. In conclusion, pigs need more time to eat meal than to eat pellets. Thus the number of pigs per feeder has to be adapted to the food presentation. Too high number of pigs per feeder impairs feeding behaviour and eventually welfare, by preventing preferential diurnal feeding activity and this may affect productivity [less ▲]

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See detailCompte-rendu de l'ouvrage de Bawin-Legros Bernadette : Familles modes d'emploi. Étude sociologique des ménages belges
Orianne, Jean-François ULg

in Recherches Sociologiques et Anthropologiques (1999), (3),

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See detailA sharp decrease of resistivity in La0.7Ca0.3Mn0.96Cu0.04O3: Evidence for Cu-assisted coherent tunneling of spin polarons
Sergeenkov, S. A.; Bougrine, Hassan ULg; Ausloos, Marcel ULg et al

in JETP Letters (1999), 69(11), 858-862

Nearly a 50% decrease of the resistivity rho(T,x) is observed upon just 4% Cu doping at the Mn site of La2/3Ca1/3Mn1-xCuxO3. When the observed phenomenon is attributed to a decrease of the spin-polaron ... [more ▼]

Nearly a 50% decrease of the resistivity rho(T,x) is observed upon just 4% Cu doping at the Mn site of La2/3Ca1/3Mn1-xCuxO3. When the observed phenomenon is attributed to a decrease of the spin-polaron energy E-sigma(x) below T-C(x), all of the data are found to be well fitted by the nonthermal coherent tunneling expression rho(T,x)=rho(0)e(-gamma M2(T,x)), assuming that the magnetization in the ferromagnetic state is given by the expression M(T,x)=M-R(x)+M-0(x)tanh{T-[root(C)(x)/T](2)-1}. The best fits through all the data points suggest M-0(x)similar or equal to root 1-xM(0)(0), M-R(x)similar or equal to x root M-0(0), and E-sigma(x)similar or equal to E-sigma(0)(1-x)(4) for the explicit x dependence of the Cu-induced modifications of the Mn-spin-dominated zero-temperature spontaneous magnetization, residual paramagnetic contribution, and spin-polaron tunneling energy, respectively, with E-sigma(0)=0.12 eV. (C) 1999 American Institute of Physics. [S0021-3640(99)00811-7]. [less ▲]

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See detailInfluence de facteurs abiotiques ou biotiques sur l'evaluation de la sensibilite d'Aphidius rhopalosiphi De Stefani-Perez a la phosalone.
Guelton, O.; Mahaut, T.; Deleu, R. et al

in Parasitica (1999), 54(2-3),

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See detailBovine herpesvirus 1-induced apoptotic cell death: role of glycoprotein D
Hanon, Emmanuel; Keil, Günther; Van Drunen Littel-Van Den Hurk, Sylvia et al

in Virology (1999), 257

Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in peripheral blood mononuclear cells and in bovine B lymphoma (BL-3) cells. Attachment but not penetration of BHV-1 is necessary to induce ... [more ▼]

Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in peripheral blood mononuclear cells and in bovine B lymphoma (BL-3) cells. Attachment but not penetration of BHV-1 is necessary to induce apoptosis in target cells, suggesting that one or more BHV-1 envelope glycoproteins could be involved in the activation of the apoptotic process. In the present study, we demonstrate that, although BHV-1 virions devoid of glycoprotein D (BHV-1 gD-/-) still bind to BL-3 cells, they are no longer able to induce apoptosis. In contrast, virions that contain glycoprotein D (gD) in the viral envelope but do not genetically encode gD (BHV-1 gD-/+) induce a level of apoptosis comparable to that produced by wild-type (wt) BHV-1. In addition, monoclonal antibodies directed against gD, but not against gB or gC, strongly reduced the high levels of apoptosis induced by BHV-1. These observations demonstrate that the induction of apoptosis is directly due to BHV-1 viral particles harboring gD in the viral envelope. Interestingly, binding of affinity-purified gD to BL-3 cells did not induce apoptosis but inhibited the ability of wt BHV-1 to induce apoptosis. Altogether, these results provide evidence for the direct or indirect involvement of gD in the mechanism by which BHV-1 induces apoptosis. [less ▲]

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See detailCryptospores and spores from the Ordovician to the Llandovery. A review
Steemans, Philippe ULg

in Acta Universitatis Carolinae. Geologica (1999), (1/2), 271--274

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See detailCurrent perspectives on developmental dysphasias
Rondal, Jean-Adolphe ULg; Comblain, Annick ULg

in Journal of Neurolinguistics (1999), 12(3-4), 181-212

This paper documents the major difficulties observed in the oral language development of individuals with mental retardation of genetic origin. The extent of inter- and within-syndrome variability is ... [more ▼]

This paper documents the major difficulties observed in the oral language development of individuals with mental retardation of genetic origin. The extent of inter- and within-syndrome variability is evaluated. More specifically, a comparative analysis of typical language phenotypes in several genetic syndromes is attempted and the possible brain underpinnings of the observed differences are envisaged. Recent cases of favorable language development in individuals with mental retardation are summarized and explanatory variables are discussed. [less ▲]

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See detailCytokinin modulates catalase activity and coumarin accumulation in in vitro cultures of tobacco
Petit-Paly, Geneviève; Franck, Thierry ULg; Brisson, Louise et al

in Journal of Plant Physiology (1999), 155(1), 9-15

Cytokinins (CKs) represent an important class of phytohormones particularly known for their antisenescence properties that might be regulated through an effect on the oxidative metabolism. In the present ... [more ▼]

Cytokinins (CKs) represent an important class of phytohormones particularly known for their antisenescence properties that might be regulated through an effect on the oxidative metabolism. In the present work, we demonstrate the effect of CKs on catalase activity in tobacco cultivated in vitro. The catalase activity observed in suspension-cultured cells decreased slightly during the first hour of CK treatment and increased thereafter to double the level detected in untreated cells. In contrast to these results, catalase activity was inhibited in shoot cultures in which the endogenous levels of CK were elevated by the introduction of the isopentenyltransferase gene or by an exogenous feeding of CK to the cultures. Interestingly, this catalase inhibition correlated with an accumulation of scopolin, an inducible coumarin. Taken together, our results show that CK modulates (directly or undirectly) catalase activity. The inverse relationship that was always found between scopolin accumulation and catalase activity is discussed in terms of vitrification and habituation. [less ▲]

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See detailPregnancy-Associated Glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from north-east of Brazil.
Melo de Sousa, Noelita ULg; Garbayo, J. M.; Figueiredo, J. R. et al

in Small Ruminant Research (1999), 32

Pregnancy diagnosis is a critical procedure for monitoring goat reproductive performance. In the present investigation, pregnancy-associated glycoprotein (PAG) profiles during pregnancy and first month ... [more ▼]

Pregnancy diagnosis is a critical procedure for monitoring goat reproductive performance. In the present investigation, pregnancy-associated glycoprotein (PAG) profiles during pregnancy and first month postpartum, as well as progesterone (P4) concentrations throughout pregnancy, were determined in native goats from the north-east of Brazil. A total of 30 goats (Moxoto, n = 19 and Caninde, n = 11) were used. Peripheral blood was collected on days 0 (Day 0 = AI), 11, 16, 18, 20, 24 and 30 after AI. Samples were subsequently collected every 2 weeks until day 145, and weekly during the first month postpartum. The levels of PAG and P4 were determined by radioimmunoassay (RIA). The profiles of PAG were not different between the breeds throughout pregnancy. However, a significant effect of the week of pregnancy (p < 0.01) and the number of fetuses (p < 0.05) in PAG concentrations were found. Lower concentrations of PAG were observed during weeks 1 and 3, compared to the rest of the gestation. Thereafter, there was a significant (p < 0.05) increase in PAG concentrations from week 5 to 7 and a significant decrease (p < 0.05) in week 9. The PAG concentrations remained relatively constant until week 17, and finally decreased slowly until parturition. After parturition, PAG concentrations decreased rapidly, and continued to decrease until week 4. During pregnancy, mean P4 concentrations had weekly variations among the animals (p < 0.01) and the period of pregnancy (p < 0.01), but P4 was not affected by the breed or number of fetuses. These results indicate that PAG and P4 concentrations were similar for Caninde and Moxoto goats and that, for both the breeds, the stage of pregnancy and the number of fetuses could influence PAG production. [less ▲]

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See detailLatest Devonian to Early Carboniferous biostratigraphy of Northern Brazil: an update.
Melo, JHG; Loboziak, S; Streel, Maurice ULg

in Bulletin du Centre de Recherches Elf Exploration Production (1999), 22(1), 13-33

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See detailIntragranular and intergranular superconducting properties of bulk melt-textured YBCO
Vanderbemden, Philippe ULg; Cloots, Rudi ULg; Ausloos, Marcel ULg et al

in IEEE Transactions on Applied Superconductivity (1999), 9(2), 2308-2311

We have investigated the superconducting properties of bulk melt-processed YBCO using different experimental techniques. First, single domain materials (i.e. containing no grain boundaries) have been ... [more ▼]

We have investigated the superconducting properties of bulk melt-processed YBCO using different experimental techniques. First, single domain materials (i.e. containing no grain boundaries) have been studied. Electrical resistivity, current-voltage characteristics, M-H loops, and A,C. susceptibility. have been measured for different field and current directions. The intragranular properties are characteristic of high-quality melt-processed YBCO material,,vith a critical current density J(c)(T=77K, B=1T//c) > 10(4) A/cm(2). The anisotropy ratio J(c)(ab)/ J(c)(c) is found to be close to 3. Both magnetic and transport measurements show that defects in the microstructure do not significantly impede the current flow inside the single domain. These results were compared to those measured on samples containing one "natural" single grain boundary which sometimes appears during the grain growth process. The intergranular properties show a much stronger current and magnetic field dependence than that measured within the grain and these differences are discussed. [less ▲]

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See detailUse of Anaesthesia Simulator: Initial Impressions of Its Use in Two Belgian University Centers
Larbuisson, Robert ULg; Pendeville, P.; Nyssen, Anne-Sophie ULg et al

in Acta Anaesthesiologica Belgica (1999), 50(2), 87-93

459 trainees in Anesthesia and Intensive Care Medicine, accompanied by fully certified specialists from several Belgian University Hospital Centers, spend at least a 3 hour session at the Anaesthesia ... [more ▼]

459 trainees in Anesthesia and Intensive Care Medicine, accompanied by fully certified specialists from several Belgian University Hospital Centers, spend at least a 3 hour session at the Anaesthesia Simulator. Each session comprises three segments: the briefing, the simulation session and the debriefing. The use of simulations allows significant individualization of the learning experience. The simulator helps to develop the capacity to understand, explain a phenomenon and to resolve problems. Another important aspect of the use of the simulator involves the trainee's "right to make mistakes". This allows to widen the spectrum of executional situations, and decreases the number of dangerous situations. Two University Centers (ULg and UCL) have each organized simulator sessions despite some differences in their approaches. The simulator is a teaching tool worthy of an obligatory role in the most up-to-date training possible of modern anesthesiologist. This is all the more important given that the current practice of anesthesiology is so complex that any error could cost a human life. [less ▲]

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See detailPreparation and pharmacological evaluation of the R- and S-enantiomers of 3-(2’-butylamino)-4H- and 3-(3’-methyl-2’-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide, two tissue selective ATP-sensitive potassium channel openers
Khelili, S.; De Tullio, Pascal ULg; Lebrun, P. et al

in Bioorganic & Medicinal Chemistry (1999), 7(8), 1513-1520

The preparation and the pharmacological evaluation of the R- and S-isomers of 3-(2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 42) and 3-(3-methyl-2-butylamino)-4H-pyrido[4,3-e]-1,2,4 ... [more ▼]

The preparation and the pharmacological evaluation of the R- and S-isomers of 3-(2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 42) and 3-(3-methyl-2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 44), two potassium channel openers, is described. Their optical purity was estimated by means of capillary electrophoresis (R- and S-BPDZ 42) and chiral HPLC (R- and S-BPDZ 44). The absolute configuration of each isomer of BPDZ 44 was deduced from crystallographic data. Pharmacological assays performed with the R- and S-isomers of BPDZ 44 revealed only slight differences in their activity on pancreatic B-cells but significant differences in their activity on vascular smooth muscle cells: the R-isomer being sixfold more potent than its corresponding S-isomer. The R-isomer of BPDZ 42 was shown to be more potent than its corresponding S-isomer on the endocrine pancreas. S-BPDZ 44 as well as R- and S-BPDZ 42 were found to exhibit tissue selectivity for the pancreatic versus the vascular smooth muscle tissue. [less ▲]

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See detailThe neural correlates of updating information in verbal working memory
Van der Linden, Martial ULg; Collette, Fabienne ULg; Salmon, Eric ULg et al

in Memory (1999), 7(5-6), 549-560

The aim of the present study was to re-examine cerebral areas subserving the updating function of the central executive with a running span task requiring subjects to watch strings of consonants of ... [more ▼]

The aim of the present study was to re-examine cerebral areas subserving the updating function of the central executive with a running span task requiring subjects to watch strings of consonants of unknown length and then to recall serially a specific number of recent items. In order to dissociate more precisely the updating process from the storage function, a four-item instead of a six-item memory load was used, contrary to our previous study (Salmon et al., 1996). In addition, a serial recall procedure was preferred to a recognition procedure in order to suppress the use of visuospatial strategies. The most significant increase of rCBF occurred in the left frontopolar cortex (Brodmann's area 10), spreading to the left middle frontal (Brodmann's area 46). Results suggest that frontopolar activation underlies an updating process in working memory. [less ▲]

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See detailProjet Solidarité : Accompagnement d'équipes éducatives dans la réalisation de leur projet d'établissement
Munten, J.; Lenoir, A.; Mouvet, Bernadette ULg

in Point sur la Recherche en Education (Le) (1999), 12

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See detailSite index curves and autecology of ash, sycamore and cherry in Wallonia (Southern Belgium)
Claessens, Hugues ULg; Pauwels, Dominique; Thibaut, André et al

in Forestry (1999), 72(3), 171-182

The work described in this article forms part of an exploratory study whose aim was to determine the main aspects of the autecology of ash (Fraxinus excelsior L.), sycamore (Acer pseudoplatanus L.) and ... [more ▼]

The work described in this article forms part of an exploratory study whose aim was to determine the main aspects of the autecology of ash (Fraxinus excelsior L.), sycamore (Acer pseudoplatanus L.) and cherry (Prunus avium L.) in Wallonia (Southern Belgium). The potential productivity of these species was studied using the site index approach (the height a crop achieves at a given age) which is the most widely accepted means for estimating site quality. As a first step, a set of site index curves were constructed from stem analysis and semi-permanent plots data using the Johnson (1935) and Schumacher (1939) model for ash and the Duplat and Tran-Ha (1986) model II for sycamore and cherry. For ash, dominant height achieved at age 50 is related to various soil-site characters (through adequate multiple regression analysis) in order to make the predictions of site quality applicable to both forested and non-forested land. Furthermore the sites expressed through soil attributes are classified in 'a site catalogue' for each species according to their productivity level. [less ▲]

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See detailPrise en charge médico-chirurgicale des enfants cardiopathes: résultats de l'expérience liégeoise
Massin, M.M.; RADERMECKER, Marc ULg; Jalali, H. et al

in Revue Médicale de Liège (1999), 54(2), 95-9

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and ... [more ▼]

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and interventional cardiac catheterization, and of the cardiac surgery in the 123 cardiac children who were referred to us during the three first years of operation of our medico-surgical team. RESULTS: 89 children underwent a catheterization, including 12 therapeutic interventions, whereas 68 were operated. In the two domains, the results are completely comparable with those of the established centers. CONCLUSIONS: The widening of the activity of paediatric cardiology and the creation of an activity of pediatric cardiac surgery in Liege appeared important to us for the quality of management of the cardiac children. This goal could be reached only if our results were comparable with those of the literature. The bet seems to be held. The ultimate objective is to contribute by an adequate policy of management to the quality of life and the health of the cardiac children. [less ▲]

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See detailDo leaching experiments affect the Sm-Nd signature of deep-sea sediments? The example of the northern North Atlantic
Innocent, Christophe; Fagel, Nathalie ULg; Stevenson, Ross

in Canadian Journal of Soil Science (1999), 79

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See detailProtochlorophyllide-NADP(+) and protochlorophyllide-NADPH complexes and their regeneration after flash illumination in leaves and etioplast membranes of dark-grown wheat
Franck, Fabrice ULg; Bereza, B.; Boddi, B.

in Photosynthesis Research (1999), 59(1), 53-61

The fast (1 min) regeneration process of the photoactive Pchlide forms after a light flash was studied in etiolated wheat leaves, and this process was simulated in vitro by incubating etioplast inner ... [more ▼]

The fast (1 min) regeneration process of the photoactive Pchlide forms after a light flash was studied in etiolated wheat leaves, and this process was simulated in vitro by incubating etioplast inner membranes of wheat with excess NADPH or NADP(+). The 77 K fluorescence spectra were recorded after flash illumination, dark incubation and a subsequent flash illumination of the samples. A non-photoactive Pchlide form with an emission maximum at 650 nm was transiently detected in leaves during regeneration of a photoactive Pchlide form with an emission maximum at 654 nm. Gaussian deconvolution of fluorescence spectra of isolated membranes showed that this 650 nm form appeared in conditions of excess NADP(+), as suggested in previous studies. Additionally a Pchlide form emitting at 638.5 nm was detected in the same conditions. The analysis of the spectra of leaves at different times after a flash indicated that these two non-photoactive forms are involved as intermediates in the regeneration of photoactive Pchlide. This regeneration is in correlation with the production of the Chlide form emitting at 676 nm. The results demonstrate that, in vivo, part of the NADPH:protochlorophyllide oxidoreductase is reloading with nonphotoactive Pchlide on a fast time-scale and that the 676 nm Chlide form is the released product of the phototransformation in this process. [less ▲]

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See detailStudies of the glutathione S-transferases activities and their localisation in Adalia bipunctata L. (Coleoptera : Coccinellidae).
Francis, Frédéric ULg; Broon, D.; Haubruge, Eric ULg

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1999), 64(3a), 349-355

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See detailNonprofit Organisations and Social Economy: Two Ways of Understanding the Third Sector
Mertens de Wilmars, Sybille ULg

in Annals of Public and Cooperative Economics = Annales de l'Economie Publique, Sociale et Coopérative (1999), 70:3

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by ... [more ▼]

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by focusing on the distinction between third sector and state-owned bodies. Next I propose to translate the non capitalist and the non governmental conditions into operational criteria. I show that these criteria vary according to the nature of the organizations (general interest versus mutual interest). This statement allows me to justifiy the emergence of two different ways of understanding the third-sector in anglo-saxon and french-speaking traditions. [less ▲]

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See detailComparison of intracellular and extracellular particles of the vaccinia virus
Vanderplasschen, Alain ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154

The construction of recombinant vaccinia viruses (VV) is definitely a promising approach to develop vaccines for pets. To produce safer VV recombinant vaccines, it is necessary to understand the ... [more ▼]

The construction of recombinant vaccinia viruses (VV) is definitely a promising approach to develop vaccines for pets. To produce safer VV recombinant vaccines, it is necessary to understand the pathogenesis of VV infection. The goal of this study was to compare the biological properties of the extracellular and intracellular infectious particles of VV. Four biological properties were investigated: (i) the binding into cellular receptor(s); (ii) the mechanism of entry; (iii) the resistance to neutralisation by antibodies; (iv) the resistance to complement lysis. The results obtained in this study emphasize the differences existing between the two infectious forms of VV and demonstrate the adaptation of the extracellular particle to its role of virus dissemination in vivo. The results of this study implicate that VV strains used as expression vector in vaccinology should be deleted for genes required for EEV morphogenesis. [less ▲]

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See detailBisphosphonates : an update
Reginster, Jean-Yves ULg

in Osteologia-Osteology (1999), 2

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See detailIdentification of mechanisms involved in the acute airway toxicity induced by parathion.
Segura, P.; Chavez, J.; Montano, L. M. et al

in Naunyn-Schmiedeberg's Archives of Pharmacology (1999), 360(6), 699-710

Organophosphates are still widely used worldwide and cause thousands of intoxications every year. In this work we investigated the mechanisms of parathion (Pth) airway toxicity, using biochemical and ... [more ▼]

Organophosphates are still widely used worldwide and cause thousands of intoxications every year. In this work we investigated the mechanisms of parathion (Pth) airway toxicity, using biochemical and functional approaches. A plethysmographic technique for unrestrained guinea pigs was used to analyze Pth-induced modifications of airway mechanics and responsiveness to acetylcholine (ACh: 0.1-3.2 mg/ml, 2-min inhalation each dose). The isolated perfused rabbit lung preparation was used to study the acute effects of Pth on airway responsiveness to ACh (10(-8)-10(-3) M), histamine (10(-8)-10(-3) M) and substance P (10(-10)-10(-6) M), pulmonary acetylcholinesterase inhibition and cytochrome P450 (P450) activity, and their modifications with previous administration of Pth (1 mg/kg s.c. daily, 7 days). We found that: (1) In guinea pigs Pth (3.2-17 mg/kg i.p.) produced a dose-dependent increase in a lung resistance index (iRL), which was greatly reverted (approximately 50%) by salbutamol (2 mg/ml, 2-min inhalation, or 10 microg/kg i.p.). This salbutamol effect was transient (5-10 min), suggesting that this bronchodilator triggered additional obstructive mechanisms. (2) Pth increased the water content in lung parenchyma samples, but not in trachea or bronchi, and augmented the respiratory secretions measured through monosaccharide content in bronchoalveolar lavage. (3) The increase in iRL was greater in female animals, probably due to a higher P450 basal activity, and completely blocked by pharmacological inhibition of P450 with piperonyl butoxide (500 mg/kg i.p.). (4) In male guinea pigs a subclinical dose of Pth (10 mg/kg i.p.) induced airway hyperresponsiveness to ACh. In isolated perfused rabbit lung Pth (10(-6) M) produced airway hyperresponsiveness to ACh and histamine, the latter prevented by atropine (10(-5) M). (5) Repetitive exposure to subclinical doses (1 mg/kg s.c.) of Pth during 1 week caused approximately 80% inhibition of P450 activity in rabbits, which was not enough, however, to prevent the functional manifestation of Pth toxicity in the airways. [less ▲]

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See detailEndogenous auxin and cytokinin levels in shoots of a nonrooting tobacco mutant
Faivre-Rampant, O.; Kevers, Claire ULg; Gaspar, Th

in Bulletin de la Societe Royale des Sciences de Liege (1999), 68(5-6), 318

[No abstract available]

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See detailLe cas clinique du mois. Rupture couverte d'un anevrysme infectieux post-typhique de l'aorte abdominale sous-renale.
SCHILS, Fabian ULg; SakalihasanN, Natzi ULg; Sente, J. M. et al

in Revue Médicale de Liège (1999), 54(10), 786-8

Mycotic aneurysms are uncommon but remain one of the most challenging clinical problems for the vascular surgeon. They are associated with high morbidity and mortality, and the clinical signs and symptoms ... [more ▼]

Mycotic aneurysms are uncommon but remain one of the most challenging clinical problems for the vascular surgeon. They are associated with high morbidity and mortality, and the clinical signs and symptoms are non specific. We report here the case of an abdominal aorta mycotic aneurysm due to salmonellosis treated by insertion of an arterial homograft. [less ▲]

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See detailLa desinfection des endoscopes par machines automatiques est-elle devenue obligatoire?
Delwaide, Jean ULg; Pelckmans, P.; Defrance, P. et al

in Revue Médicale de Bruxelles (1999), 20(1), 49-50

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See detailHarm avoidance and serotonin.
Hansenne, Michel ULg; Ansseau, Marc ULg

in Biological Psychology (1999), 51(1), 77-81

The relationships between the Tridimensional Personality Questionnaire (TPQ) and serotonergic activity has been described in some studies with controversial results. These studies have focused on specific ... [more ▼]

The relationships between the Tridimensional Personality Questionnaire (TPQ) and serotonergic activity has been described in some studies with controversial results. These studies have focused on specific patient populations rather than normal controls. Therefore, the aim of the present study is to examine the relationships between the TPQ and serotonergic activity in a group of non-patient subjects. Twenty-three normal subjects answered the TPQ, and the serotonergic activity was assessed by the prolactin response to a highly potent and selective 5-HT1a agonist (flesinoxan). A positive relationship between harm avoidance and PRL response to flesinoxan was found. This study is consistent with the hypothesized link between serotonergic activity and the harm avoidance dimension of the biosocial model of Cloninger. [less ▲]

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See detailEffects of exercise on concentrations of immunoreactive endothelin in bronchoalveolar lavage fluid of normal horses and horses with chronic obstructive pulmonary disease
Benamou, A. E.; Art, Tatiana ULg; Marlin, David J et al

in Equine Veterinary Journal. Supplement (1999), 30

Chronic obstructive pulmonary disease (COPD) represents a major cause of loss of performance in the horse. The role of endothelin (ET), a potent bronchoconstrictive and vasoactive peptide, is currently ... [more ▼]

Chronic obstructive pulmonary disease (COPD) represents a major cause of loss of performance in the horse. The role of endothelin (ET), a potent bronchoconstrictive and vasoactive peptide, is currently being investigated in asthma and other obstructive respiratory diseases in man. We have previously found elevated systemic and pulmonary endothelin levels in horses during exacerbation of COPD. In the present study, our aim was to examine possible variations in ET concentrations occurring during exercise in COPD horses. We compared the effects of intense treadmill exercise on the recovery of endothelin (ET) in the bronchoalveolar lavage fluid (BALF) as well as in arterial and venous blood, in a group of 5 healthy horses and a group of 5 COPD horses studied alternately in remission and while symptomatic. We also investigated the possible correlations between ET levels and pulmonary function tests during the study. While exercise did not affect the ET levels recovered in BALF among controls, it caused a significant increase (P = 0.02) among symptomatic COPD horses. During remission, wide variations of ET levels among horses, at rest and during exercise, made any significant interpretation difficult. No correlation could be found between exercise-induced changes in ET concentrations and pulmonary function tests or changes in arterial oxygen tension with exercise. We conclude that exercise appears to affect the release of ET by the airways in COPD horses, in contrast to healthy horses. It is still unclear, however, whether these differences relate to adjustments of lung function during exercise. [less ▲]

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See detailLes mouvements Maï Maï de la République Démocratique du Congo. D'où viennent-ils et qui sont-ils?
Kabamba, Bob ULg

in International Documentation Network on the Great African Lakes Region (1999)

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See detailEfficient Implicit Schemes for Finite Element Impact Simulation
Ponthot, Jean-Philippe ULg; Graillet, D.

in International Journal of Crashworthiness (1999), 4(3), 273-285

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See detailTEL is a sequence-specific transcriptional repressor.
Lopez, Rodolphe; Carron, Clémence; Oury, Cécile ULg et al

in Journal of Biological Chemistry (1999)

TEL is a gene frequently involved in specific chromosomal translocations in human leukemia and sarcoma that encodes a member of the ETS family of transcriptional regulators. TEL is unusual among other ETS ... [more ▼]

TEL is a gene frequently involved in specific chromosomal translocations in human leukemia and sarcoma that encodes a member of the ETS family of transcriptional regulators. TEL is unusual among other ETS proteins by its ability to self-associate in vivo, a property that is essential to the oncogenic activation of TEL-derived fusion proteins. We show here that TEL is a sequence-specific transcriptional repressor of ETS-binding site-driven transcription of model and natural promoters. [less ▲]

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See detailChemical changes and influences of rapeseed antinutritional factors on lamb physiology and performance - 1. Animal performance and thyroid histology
Derycke, G.; Mabon, N.; Mandiki, S. N. M. et al

in Animal Feed Science & Technology (1999), 81(1-2), 81-91

Sixty six Texel, Suffolk or crossbred lambs allocated to three groups were used. From the end of their first month of age till slaughtering, they were fed ad libitum with concentrates containing 0 ... [more ▼]

Sixty six Texel, Suffolk or crossbred lambs allocated to three groups were used. From the end of their first month of age till slaughtering, they were fed ad libitum with concentrates containing 0% (Control) or 25% of rapeseed meal obtained from either Samourai (Samourai) or Honk (Honk) variety. The Samourai and Honk concentrates contained 1.95 and 4.22 mmoles/g DM of glucosinolates, respectively. Lambs were weaned at 88 +/- 8 days of age and 24 +/- 5 kg of live weight and were slaughtered when their fattening state was estimated to be optimal. Apart from low concentrate intake for suckling lambs at the beginning of the experiment in the Honk, no negative effect of low glucosinolates (LG)-rapeseed meal was noted concerning animal performance. The quality of perirenal fat was better for lambs which received rapeseed meal since lower (P < 0.5) proportions of saturated fatty acids (C-10 : 0, C-12 : 0, C-14 : 0) and higher contents (P < 0.05) of some unsaturated fatty acids (C-18 : 1trans, C-22 : 2) were determined. The thyroid weight was significantly higher (P < 0.05) for the Honk (3.04 +/- 0.8 g) than in the Control (2.6 +/- 0.5 g) and the Samourai (2.7 +/- 0.6 g). Moreover, the percentages of small thyroid follicles were lower (P < 0.05) in the Honk than in the Control and Samourai groups. Conversely, the percentages of large thyroid follicles were higher (P < 0.05) in the Honk than in the two other groups. These results suggest that rapeseed meal can be used as the only protein supplement for fattening lambs despite the modification of thyroid histology by high glucosinolate content. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailLa mesure du courant critique des matériaux supraconducteurs à haute température critique
Vanderbemden, Philippe ULg

in Bulletin de l'Association des Ingénieurs sortis de l'Université de Liège (AILg) (1999), 6

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See detailCell surface immunophenotype and gelatinase activity of the human breast carcinoma cell line (MCF-7/6) with functionally defective E-cadherin.
Hlavcak, P.; Sedlakova, O.; Sedlak, J. et al

in Neoplasma (1999), 46(1), 12-6

Expression of differentiation and adhesion cell surface antigens (LewisX - CD15, CD44, syndecan 1 - CD138 and basigin/EMMPRIN - CD147) were determined on the cell surface of human breast carcinoma MCF7 ... [more ▼]

Expression of differentiation and adhesion cell surface antigens (LewisX - CD15, CD44, syndecan 1 - CD138 and basigin/EMMPRIN - CD147) were determined on the cell surface of human breast carcinoma MCF7 cells in vitro with the aid of flow cytometry and compared with that of MCF-7/6 cells, with functionally defective E-cadherin system and increased biological aggressiveness. The major cell surface alterations in MCF-7/6 cells compared with the parental MCF-7 cell line were a markedly increased CD15 (LewisX) and CD44 antigen cell surface expression on MCF-7/6 cells. There were no major differences between parental MCF-7 and MCF-7/6 cells in cell surface syndecan 1, basigin/EMMPRIN, E-cadherin and high affinity non-integrin laminin receptor expression. The constitutive cell surface gelatinase A and B activities were absent on MCF-7 and faint in MCF-7/6 cells. Both phorbol ester TPA and tumor necrosis factor TNF-alpha induced a marked up-regulation of gelatinase B only in MCF-7/6 cells. No marked differences in penetration of MCF-7 vs. MCF-7/6 cells into collagen/fibroblast matrix in vitro were observed. The increased expression of CD15 (LewisX), CD44 antigen and TNF-alpha-inducible gelatinase B on MCF-7/6 cells may represent auxiliary factors contributing to the increased biological aggressiveness of MCF-7/6 cells. [less ▲]

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See detailFrom a New-World Poetics to a New-World Vision: African America in the Works of Edouard Glissant and Caryl Phillips
Ledent, Bénédicte ULg

in Commonwealth : Essays and Studies (1999), 21(2), 29-36

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See detailSome comments on context embodiment in zootherapy
Servais, Véronique ULg

in Journal of the International Society for Anthrozoology (1999), 12(1), 5-15

The Autidolfijn Project was undertaken in 1991 in Bruges, Belgium, to asses the effect of interactions with captive dolphins on learning in autistic children. The project lasted 4 years and ended with ... [more ▼]

The Autidolfijn Project was undertaken in 1991 in Bruges, Belgium, to asses the effect of interactions with captive dolphins on learning in autistic children. The project lasted 4 years and ended with ambiguous results. Although one group of children did seem to gain learning benefits from working with the dolphins, difficulties arising from the experimental set-up itself seemed to play a role in how well the groups performed. The Autidolfijn project was intended as an experiment, but it was imperfectly controlled and consequently the data cannot be used to aswer directly the question of the therapeutic effect dolphins have on children with autism. Here the data generated by Autidolfijn are used as ethnographic data attached to a particular situation. We feel they can help us understand the difficulties involved in trying to assess experimentally the positive effects of animals on people (cf. Rowan, 1996 ; Marino and Lilienfeld, 1998 ; Nathanson, 1998). [less ▲]

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See detailMélanges Philippe Minguet
Duchesne, Jean-Patrick ULg

in Art&fact (1999), 18

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See detailRôles de la structure et de la communication familiale
Born, Michel ULg; Helin, Dominique; Chevalier, V.

in JDJ (journal droit des jeunes) (1999)

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See detailAre The Fusion Processes Involved In Birth, Life And Death Of The Cell Depending On Tilted Insertion Of Peptides Into Membranes?
Peuvot, J.; Schanck, A.; Lins, Laurence ULg et al

in Journal of Theoretical Biology (1999), 198(2), 173-81

Various peptide segments have been modeled as asymmetric amphipathic alpha-helices. Theoretical calculations have shown that they insert obliquely into model membranes. They have been named "tilted ... [more ▼]

Various peptide segments have been modeled as asymmetric amphipathic alpha-helices. Theoretical calculations have shown that they insert obliquely into model membranes. They have been named "tilted peptides". Molecular modeling results reported here also evidence the presence of tilted peptides in ADM-1 protein of Caenorhabditis elegans that may be involved in fusion events, in meltrin alpha, a protein implicated in myoblast fusion, in hemagglutinin of influenza virus, in the E2 glycoprotein of rubella virus, in the S protein of hepatitis B virus, in a subdomain of Ebola virus and in the malaria CS protein. Experimental results have indicated that tilted peptide fragments may be involved in cellular life events like sperm-egg fecondation, muscle development, protein translocation through signal sequences and cellular death caused by viral infection or parasite infestation. We speculate that membrane destabilization by these tilted peptides may be an important common step in life processes involving fusion phenomena. [less ▲]

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See detailTrophic Response Curves of Aquatic Bryophytes in Lowland Calcareous Streams
Vanderpoorten, Alain ULg; Durwael, L.

in Bryologist (1999), 102(4), 720-728

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See detailCan the standardized uptake value characterize primary brain tumors on FDG-PET?
Hustinx, Roland ULg; Smith, R. J.; Benard, F. et al

in European Journal of Nuclear Medicine (1999), 26(11), 1501-9

The aim of this study was to evaluate the usefulness of measuring the standardized uptake value (SUV) in primary brain tumors on fluorine-18 fluorodeoxyglucose (FDG) positron emission tomography (PET ... [more ▼]

The aim of this study was to evaluate the usefulness of measuring the standardized uptake value (SUV) in primary brain tumors on fluorine-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) scans. Two groups of patients were studied. Whole-brain glucose cerebral metabolic rates (wCMRs) and SUVs were obtained in 20 normal subjects. Twenty-seven patients with histology-proven malignant primary CNS tumors (high-grade gliomas n=22, primitive neuroectodermal tumors n=3, ependymomas n=2) were also studied. The degree of FDG uptake was assessed by visual inspection and thereafter regions of interest were placed over the lesion, the contralateral cortex and white matter and the whole brain. Average (avg) and maximum (max) pixel values were determined in each site. Based on these measurements, SUV, tumor to cortex (T/C) and tumor to white matter (T/WM) activity ratios were calculated. There was no correlation between wCMRs (4.55+/-0.36 mg min(-1) 100 g(-1)) and wSUVs (5.41+/-0.43) in the normal subjects (r=0.18, P=0.45). In the second group, 17 lesions were described as definitely and seven as probably malignant. However, SUVs in these tumors and in the contralateral cortex were not significantly different. Although the SUVs were generally higher in the tumor than in the contralateral white matter, there was a significant overlap between the values. The range of the SUVs was wide: 2.54-11.8 for the tumors, 2.98-9.96 for the cortex and 1.87-6.76 for the white matter. SUVs in the normal cortex were negatively correlated with blood glucose level at the time of the injection. SUVs in the whole brain and in the cortex were lower in patients previously treated by irradiation, even months after completion of the treatment. No correlation was detectable between any of the SUVs and the age of the patients, tumor type, time post injection, use of dexamethasone, patient weight, dose injected and visual score. With cutoff levels of 1.5 for T max/WM and 0.6 for T max/C, the sensitivity of the activity ratios was 74% and 96% respectively. In conclusion, SUVs do not correlate with CMRs across subjects and appear to be of limited value in characterizing brain tumors. Visual assessment and measurement of the activity ratios currently remain the most reliable methods of analysis. [less ▲]

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See detailNote sous Anvers, 23 juin 1999
Orban, Lionel ULg

in Actualités du Droit (1999)

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See detailLa socio-prévention offensive du hooliganisme
Comeron, Manuel ULg; Kellens, Georges ULg

in Revue Sport (1999), 1/2(165-166), 6-11

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See detailBovine Leukemia Virus-Induced Persistent Lymphocytosis In Cattle Does Not Correlate With Increased Ex Vivo Survival Of B Lymphocytes
Dequiedt, Franck ULg; Cantor, Gh.; Hamilton, Vt. et al

in Journal of Virology (1999), 73(2),

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See detailThe interannual change of atmospheric CO2: contribution of subtropical ecosystems?
Gérard, Jean-Claude ULg; Nemry, B.; François, Louis ULg et al

in Geophysical Research Letters (1999), 26(2), 243-246

The global terrestrial carbon cycle model CARAIB (CARbon Assimilation In the Biosphere) is used to study the response of the terrestrial ecosystems to the large scale climate variations over the period ... [more ▼]

The global terrestrial carbon cycle model CARAIB (CARbon Assimilation In the Biosphere) is used to study the response of the terrestrial ecosystems to the large scale climate variations over the period 1980-1993. The global net carbon exchange flux with the atmosphere is calculated and compared with the terrestrial contribution derived from the deconvolution of the atmospheric CO2 and delta(13)C measurements. A fairly large CO2 biospheric source is predicted during the strong El Nino events of 1982-83 and 1986-87 as a consequence of the induced global warming. The direct and indirect temperature controls of the primacy production and respiration dominate the CO2 anomaly. An analysis of the relative contribution by latitudinal bands and ecosystems shows that low-latitude vegetation dominates the variability at the El Nino time scale. In savannas, the model indicates that the interannual changes result, to a large extent, from the control of soil water content on gross primary production (GPP). In the tropical cain forests, both respiration and GPP contribute to the response of the net biospheric flux. [less ▲]

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See detailInventaire des plantes aquatiques du lit majeur de la Moselle, France, Luxembourg et Allemagne
Klein, J. P.; Vanderpoorten, Alain ULg

in Willemetia (1999), 3(18), 1-6

The inventory of the aquatic flora found in the Moselle river indicates the state of botanical knowledge at the end of 1999. It includes a total of 48 aquatic macrophytes and 20 bryophytes. Among the ... [more ▼]

The inventory of the aquatic flora found in the Moselle river indicates the state of botanical knowledge at the end of 1999. It includes a total of 48 aquatic macrophytes and 20 bryophytes. Among the recorded species two are protected in France (Luronium natans, Pilularia globulifera) and two in the Lorraine region (Potamogeton gramineus, Vallisneria spiralis). [less ▲]

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See detailDefect segmentation on 'Jonagold' apples using colour vision and a Bayesian classification method
Leemans, Vincent ULg; Magein, Hugo ULg; Destain, Marie-France ULg

in Computers & Electronics in Agriculture (1999), 23(1), 43-53

This paper shows how the information enclosed in a colour image of a bi-colour apple can be used to segment defects. A method to segment pixels, based on a Bayesian classification process, is proposed ... [more ▼]

This paper shows how the information enclosed in a colour image of a bi-colour apple can be used to segment defects. A method to segment pixels, based on a Bayesian classification process, is proposed. The colour frequency distributions of the healthy tissue and of the defects were used to estimate the probability distribution of each class. The results showed that most defects, namely bitter pit, fungi attack, scar tissue, frost damages, bruises, insect attack and scab, are segmented. However, russet was sometimes confused with the transition area between ground and blush colour. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailDeactivation mechanisms and regeneration of a bimetallic hydrodechlorination catalyst
Heinrichs, Benoît ULg; Noville, Francis ULg; Schoebrechts, Jean-Paul et al

in Studies in Surface Science and Catalysis (1999), 126

The temporal evolution of the activity and selectivity of a 1.9%Pd-3.7%Ag/SiO2 catalyst during selective hydrodechlorination of 1,2-dichloroethane into ethylene is examined. A comparison between the ... [more ▼]

The temporal evolution of the activity and selectivity of a 1.9%Pd-3.7%Ag/SiO2 catalyst during selective hydrodechlorination of 1,2-dichloroethane into ethylene is examined. A comparison between the physico-chemical properties of the fresh catalyst and of the deactivated one suggests a deactivation mechanism by poisoning or coking of Ag sites at the surface of the active Pd-Ag particles. A regeneration treatment is proposed which allows to restore the initial properties of the fresh catalyst. [less ▲]

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See detailEletriptan: a viewpoint
Schoenen, Jean ULg

in CNS Drugs (1999), 12(4), 334-335

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See detailPotential applications of PET imaging in developing novel cancer therapies.
Hustinx, Roland ULg; Eck, S. L.; Alavi, A.

in Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine (1999), 40(6), 995-1002

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See detailAnalysis of Scintigraphical Lung Images before and after Treatment of Horses Suffering from Chronic Pulmonary Disease
Votion, Dominique ULg; Ghafir, Y.; Vandenput, Sandrina ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 144(9), 232-236

Scintigraphical analysis of the ratio of inhalation (I) to perfusion (Q) was designed to determine whether chronic pulmonary disease in horses induced mismatches in I/Q and to assess whether medical ... [more ▼]

Scintigraphical analysis of the ratio of inhalation (I) to perfusion (Q) was designed to determine whether chronic pulmonary disease in horses induced mismatches in I/Q and to assess whether medical treatment would restore an I/Q distribution pattern identical to that of control horses. In addition, the results of the I/Q analysis were correlated with the alveolar-arterial PO2 difference (AaDO2). The I/Q matching found in a group of control horses was compared with the I/Q analysis of a group of diseased horses before and after their clinical signs had been treated. The analysis indicated that there was mismatching between I and Q before they were treated. The treatment improved the diseased horses, pulmonary function but there was still heterogeneity in the I/Q distribution after they had been treated. The I/Q analysis parameters were well correlated with the ratio of ventilation (VA) and pulmonary blood flow (Q), evaluated by the determination of AaDO2 [less ▲]

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See detailLes travailleurs migrants à travers la presse quotidienne de Shenzhen (1993-1999)
Florence, Eric ULg

in Techniques, Territoires et Société (1999), Vol. 36(n° 1), 285-293

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See detailSPECT and PET imaging of brain tumors.
Hustinx, Roland ULg; Alavi, A.

in Neuroimaging Clinics of North America (1999), 9(4), 751-66

The unique capacity of measuring or visualizing intracellular biochemical processes allows nuclear medicine techniques to determine functional and metabolic activities of various disorders. This article ... [more ▼]

The unique capacity of measuring or visualizing intracellular biochemical processes allows nuclear medicine techniques to determine functional and metabolic activities of various disorders. This article describes the critical role of single-photon emission computed tomography (SPECT) and positron emission tomography (PET) imaging of brain tumors beyond what can be achieved by anatomic methods. [less ▲]

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See detailAquaporin-4 water channel plays a role in the pathogenesis of cerebral edema in fulminant hepatic failure
Margulies, J. E.; Thompson, R. C.; Wycoff, K. et al

in Surgical Forum (1999), 46

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See detailThe influence of tissular cross-talking on osteoarthritis progression: role of non steroidal antiinflammatory drugs
Pelletier, JP; Reginster, Jean-Yves ULg

in Osteoarthritis and Cartilage (1999), 7

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See detailCell-Cell and Cell-Matrix Interactions During Breast Cancer Progression
Noël, Agnès ULg; Kebers, F.; Maquoi, Erik ULg et al

in Current Topics in Pathology. Ergebnisse der Pathologie (1999)

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See detailMatrix-metalloproteinases in bronchopulmonary carcinomas.
Martinella-Catusse, C.; Nawrocki, B.; Gilles, Christine ULg et al

in Histology and Histopathology (1999), 14(3), 839-43

Matrix metalloproteinases (MMPs) represent a group of enzymes involved in the degradation of most of the components of the extracellular matrix and therefore participate in tumoural invasion. MMPs ... [more ▼]

Matrix metalloproteinases (MMPs) represent a group of enzymes involved in the degradation of most of the components of the extracellular matrix and therefore participate in tumoural invasion. MMPs, especially gelatinases A and B, MT1-MMP, the activator of gelatinase A, and stromelysin-3 were found overexpressed in many cancers including bronchopulmonary carcinomas. In vivo observations revealed that fibroblasts are the principal source of production of MMPs. Some of these enzymes such as MT1-MMP and stromelysin 3, displayed a focal stromal localisation near preinvasive and invasive tumour clusters. Furthermore, some tumour cell lines were shown to stimulate the expression of MT1-MMP by fibroblasts. All these in vivo and in vitro results suggest that certain tumour cells produce diffusible factors which could influence the MMP stromal expression. Among these factors, the TCSF (Tumor Collagenase Stimulatory Factor) which is known to upregulate some MMPs in vitro could be a good candidate for this stromal regulation, since it is produced by bronchial tumour cells in vivo. In this review, we address such a cooperation between tumour and stromal cells for the production of MMPs and emphasize their necessity for tumoural progression in bronchopulmonary carcinomas [less ▲]

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See detailWomen’s Work in Rural China, Change and Continuity in an Era of Reform.
Florence, Eric ULg

in China Information (1999), Volume XIV(Summer/Spring 1999), 137-138

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See detailIdentification Of Mitochondrial Plasmid Like Dnas In Picea Abies (L.) Karst.
Chandelier, A.; Du Jardin, Patrick ULg; Avril, C. et al

in Plant Cell Reports (1999), 18(10),

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See detail16K Human Prolactin Inhibits Vascular Endothelial Growth Factor-Induced Activation of Ras in Capillary Endothelial Cells
D'Angelo, Gisela; Martini, Jean-François; Iiri, Taroh et al

in Molecular Endocrinology (Baltimore, Md.) (1999), 13(2), 692-704

Signaling pathways mediating the antiangiogenic action of 16K human (h)PRL include inhibition of vascular endothelial growth factor (VEGF)-induced activation of the mitogen-activated protein kinases (MAPK ... [more ▼]

Signaling pathways mediating the antiangiogenic action of 16K human (h)PRL include inhibition of vascular endothelial growth factor (VEGF)-induced activation of the mitogen-activated protein kinases (MAPK). To determine at which step 16K hPRL acts to inhibit VEGF-induced MAPK activation, we assessed more proximal events in the signaling cascade. 16K hPRL treatment blocked VEGF-induced Raf-1 activation as well as its translocation to the plasma membrane. 16K hPRL indirectly increased cAMP levels; however, the blockade of Raf-1 activation was not dependent on the stimulation of cAMP-dependent protein kinase (PKA), but rather on the inhibition of the GTP-bound Ras. The VEGF-induced tyrosine phosphorylation of the VEGF receptor, Flk-1, and its association with the Shc/Grb2/Ras-GAP (guanosine triphosphatase-activating protein) complex were unaffected by 16K hPRL treatment. In contrast, 16K hPRL prevented the VEGF-induced phosphorylation and dissociation of Sos from Grb2 at 5 min, consistent with inhibition by 16K hPRL of the MEK/MAPK feedback on Sos. The inhibition of Ras activation was paralleled by the increased phosphorylation of 120 kDa proteins comigrating with Ras-GAP. Taken together, these findings show that 16K hPRL inhibits the VEGF-induced Ras activation; this antagonism represents a novel and potentially important mechanism for the control of angiogenesis. [less ▲]

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See detailLes convergences culturelles dans les sociétés pluriethniques de K. Fall et al.
Martiniello, Marco ULg

in Recherches Sociologiques (1999), 2

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See detailImpaired motor coordination and Purkinje cell excitability in mice lacking calretinin
Schiffmann, Serge N.; Cheron, Guy; Lohoff, Ann et al

in Proceedings of the National Academy of Sciences of the United States of America (1999), 96

n the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium ... [more ▼]

n the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca2+]i). Ca2+ binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr-/-) are impaired in tests of motor coordination. An impairment in Ca2+ homeostasis in Cr-/- Purkinje cells was supported by the high Ca2+-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr-/- alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology [less ▲]

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See detailActualites therapeutiques 1999.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(9), 725-34

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The originality of each molecule as well as ... [more ▼]

The most important drugs registered and/or launched in Belgium during the last year in the various disciplines of internal medicine will be briefly described. The originality of each molecule as well as its modalities of appropriate use in clinical practice will be stressed. [less ▲]

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See detailAnimal model genetic evaluation of type traits for five dairy cattle breeds
Gengler, Nicolas ULg; Wiggans, G. R.; Wright, J. R.

in Journal of Dairy Science (1999), 82(6), 13501-135022

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See detailBreeding for "low-gossypol seed and high-gossypol plants" in upland cotton. Analysis of tri-species hybrids and backcross progenies using AFLPs and mapped RFLPs.
Vroh Bi, I.javascript:void(0); Maquet, A.; Baudoin, Jean-Pierre ULg et al

in Theoretical and Applied Genetics (1999), 99(7-8),

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See detailThe YOL112w and YNL293w genes encodes activities related to cellular growth polarization in Saccharomyces cerevisiae
Bouchat, Olivier; Bach, Stéphane; Portetelle, Daniel ULg et al

in Archives Internationales de Physiologie et de Biochimie (1999), 107

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See detailMolecular Cloning And Characterization Of The Enzyme Udp-Glucose: Protein Transglucosylase From Potato
Bocca, Sn.; Kissen, R.; Rojas-Beltran, Ja. et al

in Plant Physiology and Biochemistry (1999), 37(11),

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See detailObzor verkhnego paleolita Moldavii
Otte, Marcel ULg; Noiret, Pierre ULg; López Bayón, Ignacio

in Stratum Plus (1999), 1

The existing in Moldova Aurignacian industries of the lamellar type are represented by the stone collections of two kinds: with leaf-shaped points and backed points with a concave edge. Together with this ... [more ▼]

The existing in Moldova Aurignacian industries of the lamellar type are represented by the stone collections of two kinds: with leaf-shaped points and backed points with a concave edge. Together with this the Corpach IV industry which includes backed points (segments) must have been related to Gravette. Other industries should not be considered separately and apparently can be grouped under the title "industries de type Paléolithique supérieur ancien à pointes foliacées". They are supposed to spring from the Middle Palaeolithic industries with leaf-shaped pieces (from Micoquian of Central Europe), that were further developed in the Aurignacian direction. If the Aurignacian of Moldova has evidently a foreign origin, then the Gravette traditions go back to some related centers. It is reflected in the compatibilty of backed pieces and leaf-shaped points in one and the same industry, dated back to the early stage of the Upper Palaeolithic. It is clearly seen in the collections of lower layers of Molodova V where homogeneousness of the complex is evident starting with the tenth layer. The appearance of some new types of tools (for instance, shouldered points) in the upper layers of this site does not fail the general stability and the cultural originality of the industry. [less ▲]

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See detailA Basis for Using Fire Modeling with 1-D Thermal Analyses of Partitions to Simulate 2-D and 3-D Structural Performance in Real Fires
Cooper, Len; Franssen, Jean-Marc ULg

in Fire Safety Journal (1999), 33

A basis is presented for identifying partition design for which: 1) the use of one-dimensional thermal analysis in the fire modeling part of the problem would lead to reliable simulations of the fire ... [more ▼]

A basis is presented for identifying partition design for which: 1) the use of one-dimensional thermal analysis in the fire modeling part of the problem would lead to reliable simulations of the fire environment, and 2) the resulting simulations can be used to provide the necessary boundary conditions input to solve the thermal/structural part of the problem, using two- or three-dimensional analyses, as required. [less ▲]

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See detailIonization potentials of atoms and ions from lithium to tin (Z = 50)
Biémont, Emile ULg; Frémat, Y.; Quinet, Pascal ULg

in At. Data Nucl. Data Tables (1999), 71

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See detailEffet de la paille de froment et de la sciure d’épicéa sur la dégradation de l’azote urinaire en présence d’uréase
Nimenya, H.; Delaunois, Annie; Bloden, Serge ULg et al

in Annales de Médecine Vétérinaire (1999), 143

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See detailCLF Based designs with robustness to dynamic input uncertainties
Jankovic, Mrdjan; Sepulchre, Rodolphe ULg; Kokotovic, Petar

in Systems & Control Letters (1999), 37

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See detailLifetimes and transition probabilities in In II
Biémont, Emile ULg; Zeippen, C. J.

in At. Data Nucl. Data Tables (1999), 72

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See detailGrowth hormone binding protein (GHBP) during lactation in cows.
Renaville, Robert ULg; Prandi, A.; Massart, Serge et al

in Archivio Veterinario Italiano (1999), 50

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See detailAbsces mammaire et toxemie gravidique revelateurs d'une tuberculose multiresistante.
GACH, Olivier ULg; CORHAY, Jean-Louis ULg; Lousberg, Laurent ULg et al

in Revue des Maladies Respiratoires (1999), 16(5), 842-5

We report here the case of a 33-year-old woman admitted in hospital for eclampsia. An infectious course led to the diagnosis of tuberculosis breast abscess with laryngitis and tuberculous bilateral ... [more ▼]

We report here the case of a 33-year-old woman admitted in hospital for eclampsia. An infectious course led to the diagnosis of tuberculosis breast abscess with laryngitis and tuberculous bilateral excavated bronchopneumonia. The isolated strain demonstrated resistance to the principals antituberculous agents. However outcome was favorable after cesarean and treatment adapted to sensitivity studies. [less ▲]

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See detailEtude du role de Clostridium perfringens dans l'entérotoxémie bovine
Manteca, Christophe; Daube, Georges ULg; Mainil, Jacques ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(6, Pt 2),

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as ... [more ▼]

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as clinical signs, if any. The most pronounced lesion is a necrohaemorrhagic enteritis of the jejunum, the ileum, and sometimes the colon. Suckling beef calves are the most frequently affected ones. In 67% of the 78 field cases investigated, some kind of stress was observed 24 to 36 hours prior to the death: change in diet or pasture, vaccination... The most frequently isolated bacteria, and the one isolated in highest numbers, was non-sporulated non-enterotoxigenic toxinotype A Clostridium perfringens. Reproduction of the lesions was successful in a ligated intestinal loop assay in one calf with a few of these strains, more especially with one of them, which was shown later to produce another recently described toxin, the beta 2 toxin. A role for this beta 2 toxin in bovine enterotoxaemia is thus speculated for future research. [less ▲]

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See detailLa mora debitoris est-elle une faute?
Gerkens, Jean-François ULg

in Mélanges offerts en l'Honneur de Fritz Sturm (1999), I

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See detailL'opposition à mariage doit être confinée dans des limites strictes. Note sous Civ. Bruxelles, 24 septembre 1997
Genicot, Gilles ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (1999)

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See detailLys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures
Alvarez, Marco; Wouters, Johan; Maes, Dominique et al

in Journal of Biological Chemistry (1999), 274(27), 19181-7

The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His12-Lys13 pair instead of the Asn12-Gly13 pair ... [more ▼]

The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His12-Lys13 pair instead of the Asn12-Gly13 pair normally present in mesophilic TIMs. His12 in bTIM was proposed to prevent deamidation at high temperature, while the precise role of Lys13 is unknown. To investigate the role of the His12 and Lys13 pair in the enzyme's thermoadaptation, we reintroduced the "mesophilic residues" Asn and Gly into both thermophilic TIMs. Neither double mutant displayed diminished structural stability, but the bTIM double mutant showed drastically reduced catalytic activity. No similar behavior was observed with the tTIM double mutant, suggesting that the presence of the His12 and Lys13 cannot be systematically correlated to thermoadaptation in TIMs. We determined the crystal structure of the bTIM double mutant complexed with 2-phosphoglycolate to 2.4-A resolution. A molecular dynamics simulation showed that upon substitution of Lys13 to Gly an increase of the flexibility of loop 1 is observed, causing an incorrect orientation of the catalytic Lys10. This suggests that Lys13 in bTIM plays a crucial role in the functional adaptation of this enzyme to high temperature. Analysis of bTIM single mutants supports this assumption. [less ▲]

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See detailLa vaccination contre le virus respiratoire syncytial bovin
Thiry, Etienne ULg; Pastoret, Paul-Pierre ULg; Valarcher, J. F. et al

in Bulletin des Groupements Techniques Vétérinaires (1999), 5

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