References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Epidermolyse staphylococcique aigue chez une quinquagenaire
Fumal, I.; Paquet, Philippe ULg; JACOB, ERIC et al

in Revue Médicale de Liège (2001), 56(11), 745-747

Staphylococcal scalded skin syndrome is a bullous disease induced by bacterial exfoliative exotoxins. Children are most often affected. The prognosis is worse in adults and calls for treatment in an ... [more ▼]

Staphylococcal scalded skin syndrome is a bullous disease induced by bacterial exfoliative exotoxins. Children are most often affected. The prognosis is worse in adults and calls for treatment in an intensive care unit. We report a case in a quinquagenerian woman who suffered from angina a few days before the bullous erythroderma. [less ▲]

Detailed reference viewed: 63 (0 ULg)
Full Text
Peer Reviewed
See detailInvisible fences: Prose poetry as a genre in French and American literature
Delville, Michel ULg

in Modernism-Modernity (2001), 8(4), 705-706

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailScantling Optimization Based on Convex Linearizations and a Dual Approach
Rigo, Philippe ULg; Fleury, Claude ULg

in Marine Structures (2001)

Detailed reference viewed: 68 (18 ULg)
Full Text
Peer Reviewed
See detailAssociation of telechelic ionomers in apolar solvents
Chassenieux, Christophe; Nicolai, Taco; Tassin, Jean-François et al

in Macromolecular Rapid Communications (2001), 22

The associative properties of well-defined halatotelechelic ionomers in an apolar solvent were studied by means of dynamic mechanical spectroscopy, small-angle X-ray scattering, as well as static and ... [more ▼]

The associative properties of well-defined halatotelechelic ionomers in an apolar solvent were studied by means of dynamic mechanical spectroscopy, small-angle X-ray scattering, as well as static and dynamic light scattering. The effect of end-functionalisation was investigated by comparing results on polystyrene (PS) and polyisoprene (PI) precursors using PS and PI of the same molar mass modified at one or both ends with sulfonate groups. The molar-mass dependence was investigated by comparing difunctionalised samples with different molar masses and the influence of the backbone by comparing polystyrene and polyisoprene. Experimental data suggest a relatively simple model for the association of this material. At low concentrations, the polymer chains organise themselves into well-defined reversed micelles, so-called flowers. Above a certain concentration C+, the flowers associate into larger aggregates according to an open association mechanism. At a critical concentration Cgel, the aggregates fill the whole space and a transient network is formed most likely by a percolation process. A semi-quantitative description of the associating process can be obtained by considering an open association mechanism properly combined with inter-particle interactions. [less ▲]

Detailed reference viewed: 38 (2 ULg)
Full Text
Peer Reviewed
See detailDispenses de permis d'urbanisme en Région wallonne
Delnoy, Michel ULg

in Immobilier (2001), 18

Detailed reference viewed: 21 (4 ULg)
Full Text
Peer Reviewed
See detailpH-dependent micellization of poly(2-vinylpyridine)-block-poly((dimethylamino) ethyl methacrylate) diblock copolymers
Gohy, Jean-François; Antoun, Sayed; Jérôme, Robert ULg

in Macromolecules (2001), 34(21), 7435-7440

A series of poly(2-vinylpyridine)-block-poly((dimethylamino)ethyl methacrylate) diblock copolymers (P2VP-b-PDMAEMA) have been synthesized by living anionic polymerization of the comonomers. Dynamic light ... [more ▼]

A series of poly(2-vinylpyridine)-block-poly((dimethylamino)ethyl methacrylate) diblock copolymers (P2VP-b-PDMAEMA) have been synthesized by living anionic polymerization of the comonomers. Dynamic light scattering (DLS) has been used to study the associative behavior of these copolymers in water as a function of copolymer composition, pH, ionic strength, and temperature. Three association regimes are observed. At low pH, loose aggregates are formed, although both the P2VP and PDMAEMA blocks are protonated. At intermediate pH, micelles are observed that consist of an uncharged hydrophobic P2VP core surrounded by a protonated PDMAEMA corona. At higher pH, the PDMAEMA corona is essentially uncharged and collapses at temperatures higher than 40 °C, which results in unstable micelles. The micelles observed by transmission electron microscopy are spherical. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailNeural and cognitive bases of upper limb apraxia in corticobasal degeneration
Peigneux, Philippe ULg; Salmon, Eric ULg; Garraux, Gaëtan ULg et al

in Neurology (2001), 57(7), 1259-1268

OBJECTIVE: To investigate the neural and cognitive bases of upper limb apraxia in corticobasal degeneration (CBD). METHODS: Eighteen patients with CBD underwent a cognitive neuropsychological assessment ... [more ▼]

OBJECTIVE: To investigate the neural and cognitive bases of upper limb apraxia in corticobasal degeneration (CBD). METHODS: Eighteen patients with CBD underwent a cognitive neuropsychological assessment of apraxia and resting [(18)F]-fluorodeoxyglucose PET scanning. Two complementary measures of apraxia were computed for each modality of gesture production. First, a performance score measured error frequency during gesture execution. Second, as a more stringent test of the integrity of the praxis system, the correction score measured the patient's ability to correct his or her errors on a second attempt. For each measure type, a cut-off score for the presence of apraxia was defined with regard to healthy controls. Using each cut-off score, the regional cerebral glucose metabolism of patients with CBD with apraxia (i.e., performing below cut-off score) was compared with that of patients with CBD without apraxia. RESULTS: Mean performance scores were below normal values in all modalities. Anterior cingulate hypometabolism predominated in patients with CBD who performed below the cut-off performance score. At variance, mean correction scores were below normal values for gesture imitation only. Hypometabolism in superior parietal lobule and supplementary motor area characterized patients with CBD who were unable to correct their errors at the same rate as control subjects did. CONCLUSIONS: Distinct neural networks underlie distinct aspects of the upper limb apraxic deficits in CBD. Extending previous findings of gesture production deficits in CBD, the use of complementary measures of apraxic behavior discloses a visuoimitative upper limb apraxia in CBD, underlain by a metabolic decrease in a parietofrontal neural network. [less ▲]

Detailed reference viewed: 68 (3 ULg)
Full Text
Peer Reviewed
See detailValeur d'expropriation des sites d'intérêt régional en RW
Delnoy, Michel ULg

in Immobilier (2001), 17

Detailed reference viewed: 29 (1 ULg)
Full Text
Peer Reviewed
See detailA first detailed study of the colliding wind WR+O binary WR 30a
Gosset, Eric ULg; Royer, P.; Rauw, Grégor ULg et al

in Monthly Notices of the Royal Astronomical Society (2001), 327

We present a detailed, extensive investigation of the photometric and spectroscopic behaviour of WR 30a. This star is definitely a binary system with a period around 4.6d. We propose the value P=4.619d ... [more ▼]

We present a detailed, extensive investigation of the photometric and spectroscopic behaviour of WR 30a. This star is definitely a binary system with a period around 4.6d. We propose the value P=4.619d (sigma=0.002d). The identification of the components as WO4+O5((f)) indicates a massive evolved binary system; the O5 component is a main-sequence or, more likely, a giant star. The radial velocities of the O star yield a circular orbit with an amplitude K[SUB]O[/SUB]=29.9 (sigma=2.1)kms[SUP]-1[/SUP] and a mass function of 0.013 (sigma=0.003)M[SUB]solar[/SUB]. The spectrum of WR 30a exhibits strong profile variations of the broad emission lines that are phase-locked with the orbital period. We report the detection of the orbital motion of the WO component with K[SUB]WO[/SUB]=189kms[SUP]-1[/SUP], but this should be confirmed by further observations. If correct, it implies a mass ratio M[SUB]WO[/SUB] /M[SUB]O[/SUB]=0.16. The star exhibits sinusoidal light variations of amplitude 0.024mag peak-to-peak with the minimum of light occurring slightly after the conjunction with the O star in front. On the basis of the phase-locked profile variations of the Civ lambda4658 blend in the spectrum of the WO, we conclude that a wind-wind collision phenomenon is present in the system. We discuss some possibilities for the geometry of the interaction region. [less ▲]

Detailed reference viewed: 23 (6 ULg)
Full Text
Peer Reviewed
See detailHighly regular polyampholytic structures adsorbed directly from solution
Mahltig, Boris; Müller-Buschbaum, P.; Wolkenhauer, M. et al

in Journal of Colloid and Interface Science (2001), 242(1), 36-43

This article concerns the adsorption of the diblock polyampholyte poly(methacrylic acid)-block-poly((dimethylamino)ethyl methary-late) (PMAA-b-PDMAEMA) from aqueous solution on silicon substrates. The ... [more ▼]

This article concerns the adsorption of the diblock polyampholyte poly(methacrylic acid)-block-poly((dimethylamino)ethyl methary-late) (PMAA-b-PDMAEMA) from aqueous solution on silicon substrates. The investigated polyampholyte is characterized by a small molecular weight around 15,000 g/mol and a big positively charged PDMAEMA block. The adsorbed amount determined by ellipsom-etry was str ongly influenced by the pH of the adsorption solution. Using dynamic light scattering polyampholytic structures with diameters around 50 nm were found in aqueous solution. The hy-drodynamic diameter was hardly affected by changing the pH of the polymer solution. Analogous regular structures were also found by scanning force microscopy (SFM) and grazing incidence, small angle X-ray scattering (GISAXS) at the silicon surface after the adsorption process. While SFM provides a topographical image of a small part of the adsorbed polyampholytic layer; GISAXS was used to get a statistical description of the lateral surface structures. The adsorbed structures were highly regular and their sizes were nearly pH independent over a lar ge pH region. Only directly at the isoelectric point of the polyampholyte larger adsorbed structures were observed. Compared with earlier investigated PMAA-b-PDMAEMA systems we are now able to prepare highly regular polyampholytic structures at silicon surfaces. There are two kinds of interactions for the adsorbed micelles. First, the charged block of the chains is directly attracted to the substrate via electrostatic interactions, while the uncharged part of the chains is only hydrophobically attracted via the hydrophobic core of the adsorbed micelle. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Peer Reviewed
See detailThe role of proton precipitation in the excitation of auroral FUV emissions
Hubert, Benoît ULg; Gérard, Jean-Claude ULg; Bisikalo, D. V. et al

in Journal of Geophysical Research (2001), 106

Far ultraviolet remote sensing from a high-altitude satellite is extensively used to image the global aurora, derive its energetics, and follow its dynamical morphology. It is generally assumed that the ... [more ▼]

Far ultraviolet remote sensing from a high-altitude satellite is extensively used to image the global aurora, derive its energetics, and follow its dynamical morphology. It is generally assumed that the observed emissions are dominated by the interaction of the precipitated electrons with the thermospheric constituents. A model to calculate far ultraviolet emissions excited by auroral electrons and protons and the secondary electrons they generate has been used to calculate the volume excitation rate of the H I Ly-alpha, O I 1304 and 1356 Å, N I 1493 Å multiplets, and the N[SUB]2[/SUB] Lyman-Birge-Hopfield (LBH) bands. The characteristic energy and the energy flux are derived from the observed statistical distribution of precipitated protons and electrons. This model is applied to the midnight aurora, the noon cusp, and a proton-dominated aurora for moderately disturbed conditions. We show that in the first two cases, direct electron impact dominates the vertically integrated emission rate over the proton component, although proton excitation plays an important role at some altitudes in the daytime cusp. In afternoon regions of the auroral zone near the auroral boundary, secondary electrons due to proton ionization are the main source of FUV emissions. The energy dependence of the efficiency of LBH band emission viewed from high altitude is calculated for electron and proton precipitations. Maps of the N[SUB]2[/SUB] LBH emission excited by both components are obtained, and regions of proton-dominated auroral emission are identified. It is found that the distribution of the ratio of proton-induced to electron-induced brightness resembles maps of the ratio of the respective precipitated energy fluxes. Proton-dominated FUV emissions are thus located in a C-shaped sector extending from prenoon to midnight magnetic local times with a maximum proton contribution near the equatorward boundary of the statistical electron oval. The distribution of the Ly-alpha/LBH intensity ratio is found to mimic the ratio of the proton flux/total energy flux, although it is insufficient by itself to accurately determine the relative fraction of auroral energy carried by the protons. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailCharacteristics of Necrotoxigenic Escherichia Coli Isolated from Septicemic and Diarrheic Calves between 1958 and 1970
Van Bost, Sigrid; Babe, Marie Hélène; Jacquemin, Etienne et al

in Veterinary Microbiology (2001), 82(4), 311-20

A total of 434 Escherichia coli isolated from septicemic calves between 1958 and 1965 and 430 E. coli isolated from diarrheic calves between 1967 and 1970 were studied by colony hybridisation and PCR ... [more ▼]

A total of 434 Escherichia coli isolated from septicemic calves between 1958 and 1965 and 430 E. coli isolated from diarrheic calves between 1967 and 1970 were studied by colony hybridisation and PCR assays for the presence of the cnf1- and the cnf2-like genes. They were also studied for the presence of genes coding for putative virulence factors associated with the CNF toxins including F17-, Pap- and Sfa-fimbrial adhesins and the recently described CDT-III toxin and AfaVIII-afimbrial adhesin. Thirty (7%) of the 434 septicemic strains were positive for CNF by colony hybridisation. Twenty-six were confirmed as necrotoxigenic E. coli type 2 (NTEC2) and four as NTEC1 by PCR. Thirty-five (8%) of the 430 diarrheic strains were positive for CNF by colony hybridisation. Five of them were studied by PCR and confirmed as NTEC1. The 26 septicemic NTEC2 strains and 20 of the 35 diarrheic NTEC including three of the five NTEC1 were positive for CDT-III. All adhesins studied were present in NTEC as well as in non-NTEC. NTEC1 were mainly Pap-, Sfa- and/or Afa8-positive, whereas NTEC2 were mainly F17- and/or Afa8-positive. This study shows that necrotoxigenic E. coli with their associated adhesins and toxins were present in calves as early as 1958, but their prevalence seems to have increased since that time. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Peer Reviewed
See detailFifth supplement to the catalogue of observed periods of Ap stars
Renson, Pierre ULg; Catalano, F. A.

in Astronomy and Astrophysics (2001), 378

New data on the periods of Ap stars with references are presented. 161 further stars are introduced for which a periodic variability has recently been discovered or it was known before but it was not ... [more ▼]

New data on the periods of Ap stars with references are presented. 161 further stars are introduced for which a periodic variability has recently been discovered or it was known before but it was not reported in previous issues of this catalogue. For many stars also present in previous issues of the catalogue new determinations of the periods are given. Recently attributed variable star names are also quoted. Tables 1 to 3 are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A&A/378/113 [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailSelf-aggregation of poly(methyl methacrylate)-block-poly(sulfonated glycidyl methacrylate) copolymers
Gohy, Jean-François; Antoun, Sayed; Jérôme, Robert ULg

in Polymer (2001), 42(21), 8637-8645

The self-aggregation of a series of amphiphilic poly(methyl methacrylate)-block-poly(sulfonated glycidyl methacrylate) (PMMA-b-PSGMA) copolymers in water has been studied by dynamic light scattering (DLS ... [more ▼]

The self-aggregation of a series of amphiphilic poly(methyl methacrylate)-block-poly(sulfonated glycidyl methacrylate) (PMMA-b-PSGMA) copolymers in water has been studied by dynamic light scattering (DLS), size exclusion chromatography (SEC) and surface tension measurements. All the copolymers considered in this study form micelles which, however, aggregate into larger structures as shown by DLS and SEC. These larger structures dissociate into smaller ones upon increasing the temperature. The extent of this dissociation process is strongly dependent on the copolymer composition. It is large as the content of the hydrophilic block is high. [less ▲]

Detailed reference viewed: 52 (3 ULg)
Full Text
Peer Reviewed
See detailMetallocene catalyzed polymerization of ethylene in the presence of graphite. 2. Structure and electrical properties of the composites
Pluta, Miroslaw; Alexandre, Michaël ULg; Blacher, Silvia ULg et al

in Polymer (2001), 42(22), 9293-9300

Structure and electrical properties of conducting polyethylene/graphite composites have been studied in relation to the preparation method, i.e. (i) the polymerization-filling technique (PFT), in which ... [more ▼]

Structure and electrical properties of conducting polyethylene/graphite composites have been studied in relation to the preparation method, i.e. (i) the polymerization-filling technique (PFT), in which the polyolefinic chains are growing from the graphite surface, (ii) the slurry polymerization of ethylene in the presence of untreated graphite, and (iii) the mechanical blending of preformed polyethylene and graphite. The extent of the filler dispersion depends on the method used for the composite preparation. Moreover, the graphite particles can be oriented by the molding of the samples used for the measurement of the electrical properties. This orientation is as pronounced as the melt viscosity of polyethylene is low, this characteristic feature changing with the preparation method. These structural details have consequences on the electrophysical properties and the percolation threshold. Finally, the thermal dependence of the electrical resistivity has been studied. [less ▲]

Detailed reference viewed: 64 (2 ULg)
Full Text
Peer Reviewed
See detailDid Psychrophilic Enzymes Really Win the Challenge?
Zecchinon, Laurent ULg; Claverie, P.; Collins, T. et al

in Extremophiles : Life Under Extreme Conditions (2001), 5(5), 313-21

Organisms living in permanently cold environments, which actually represent the greatest proportion of our planet, display at low temperatures metabolic fluxes comparable to those exhibited by mesophilic ... [more ▼]

Organisms living in permanently cold environments, which actually represent the greatest proportion of our planet, display at low temperatures metabolic fluxes comparable to those exhibited by mesophilic organisms at moderate temperatures. They produce cold-evolved enzymes partially able to cope with the reduction in chemical reaction rates and the increased viscosity of the medium induced by low temperatures. In most cases, the adaptation is achieved through a reduction in the activation energy, leading to a high catalytic efficiency, which possibly originates from an increased flexibility of either a selected area of or the overall protein structure. This enhanced plasticity seems in return to be responsible for the weak thermal stability of cold enzymes. These particular properties render cold enzymes particularly useful in investigating the possible relationships existing between stability, flexibility, and specific activity and make them potentially unrivaled for numerous biotechnological tasks. In most cases, however, the adaptation appears to be far from being fully achieved. [less ▲]

Detailed reference viewed: 26 (0 ULg)
Full Text
Peer Reviewed
See detailNeuroprotection with Anaesthetic Agents
Hans, Pol ULg; BONHOMME, Vincent ULg

in Current Opinion in Anaesthesiology (2001), 14(5), 491-6

The term 'neuroprotection' is used to refer to any prophylactic measure that is initiated during the peri-ischaemic period in order to improve neuronal survival. Cell death after ischaemia has an ... [more ▼]

The term 'neuroprotection' is used to refer to any prophylactic measure that is initiated during the peri-ischaemic period in order to improve neuronal survival. Cell death after ischaemia has an immediate, necrotic and a delayed, apoptotic origin. The major biochemical mechanisms that are involved in this process include transmembrane ionic fluxes and intracellular calcium increase, excitotoxicity, free radical formation, peroxynitrite production, release of inflammatory mediators, mitochondrial dysfunction, cytochrome c release, and activation of caspases and transcription factors. Strategies of neuroprotection essentially impact on those biochemical pathways. The label 'neuroprotectant' requires that the therapy has basic properties that are consistent with potential mechanisms of neuroprotection, and that conclusive results are available from animal studies that can be converted into clinical benefit. The present review focuses on neuroprotective effects of anaesthetics and is based on the most recently published reports. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailWhy is stromatactis so rare in Mesozoic carbonate mud mounds?
Neuweiler, F.; Bourque, P. A.; Boulvain, Frédéric ULg

in Terra Nova (2001), 13(5), 338-346

The sedimentary-diagenetic structure stromatactis is wide-spread in Palaeozoic spiculitic carbonate mud mounds, but occurs only sporadically in Mesozoic sponge carbonate mud mounds. Comparative analysis ... [more ▼]

The sedimentary-diagenetic structure stromatactis is wide-spread in Palaeozoic spiculitic carbonate mud mounds, but occurs only sporadically in Mesozoic sponge carbonate mud mounds. Comparative analysis of Palaeozoic and Mesozoic stromatactis limestones suggests that this variation results from the degree of siliceous sponge skeletal rigidity and the amount of internal sediment accumulation in the original cavity network. Partial to entire filling by internal sediment resulted in a continuum, from a small amount of internal sediment and large amount of cement (stromatactis, common in the Palaeozoic), to only internal sediments (aborted stromatactis, common in the Mesozoic). These observations match independent lines of evidence concerning the siliceous sponge evolution and sediment recycling (e.g. bioerosion) across the Palaeozoic to Mesozoic biotic revolution. [less ▲]

Detailed reference viewed: 42 (6 ULg)
Peer Reviewed
See detailAnthrax et charbon
Arrese, J. E.; Pierard-Franchimont, Claudine ULg; Pierard, Gérald ULg

in Revue Médicale de Liège (2001), 56(10), 685-8

Anthrax is a name given in French language to two distinct infectious diseases. One corresponds to carbuncle which is a collection of boils. The other one corresponds to the English term anthrax. This ... [more ▼]

Anthrax is a name given in French language to two distinct infectious diseases. One corresponds to carbuncle which is a collection of boils. The other one corresponds to the English term anthrax. This condition has a clinical presentation and an outcome that vary according to the inoculation site being cutaneous, pulmonary or digestive, and to the bacterial and toxin spread in the body. [less ▲]

Detailed reference viewed: 9 (3 ULg)
Full Text
Peer Reviewed
See detailOsteo-onychodysplasie héréditaire (Nail Patella Syndrome)
Vanhooteghem, Olivier ULg; Henrijean, A.; Richert, Bertrand ULg et al

in Annales de Dermatologie et de Vénéréologie (2001), 128(10, Pt 1), 1063-7

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailExtensive inflammatory pseudotumor of the pituitary.
Hansen, Isabelle ULg; Petrossians, Patrick ULg; Thiry, Albert ULg et al

in Journal of Clinical Endocrinology and Metabolism (2001), 86(10), 4603-4610

A 40-yr-old female presented with an extensive lesion of the sellar area and the sphenoid sinus, spreading to the optic nerves and associated with pachymeningitis. Histological findings were consistent ... [more ▼]

A 40-yr-old female presented with an extensive lesion of the sellar area and the sphenoid sinus, spreading to the optic nerves and associated with pachymeningitis. Histological findings were consistent with an inflammatory pseudotumor, and steroid treatment allowed the disappearance of all the lesions. Inflammatory pseudotumors of the pituitary are very rare. This case appears unique with regard to the extension of the lesions and the dramatic response to medical treatment. The differential diagnosis of inflammatory lesions of the pituitary is difficult. It relies mainly on histological analysis and includes sarcoidosis, Wegener's granulomatosis, histiocytosis (Langerhans, Rosai-Dorfman, and Erdheim-Chester diseases) and lymphocytic hypophysitis. [less ▲]

Detailed reference viewed: 40 (1 ULg)
Peer Reviewed
See detailFactor Xiiia-Positive Dendrocyte Rarefaction in Ehlers-Danlos Syndrome, Classic Type
Hermanns-Lê, Trinh ULg; Pierard, Gérald ULg

in American Journal of Dermatopathology (2001), 23(5), 427-30

The Ehlers-Danlos syndrome (EDS) represents a heterogeneous group of connective tissue disorders recognized by distinct clinical, microscopic, and biochemical aspects. In particular, some histologic and ... [more ▼]

The Ehlers-Danlos syndrome (EDS) represents a heterogeneous group of connective tissue disorders recognized by distinct clinical, microscopic, and biochemical aspects. In particular, some histologic and ultrastructural clues have been reported in the literature. We present a novel immunohistochemical aid to the diagnosis of the most frequent type of EDS. Factor XIIIa-positive dendrocytes are almost absent in the reticular dermis and markedly reduced in number and size in the adventitial dermis. By contrast, the densities of vimentin-positive cells and CD34-positive cells were unremarkable. The biologic significance of this finding is unknown. However, at least a subset of dermal dendrocytes interacts with fibroblasts. This mechanism could be affected in the classic form of EDS. [less ▲]

Detailed reference viewed: 24 (5 ULg)
Full Text
Peer Reviewed
See detailDifferential expression of the GTL2 gene within the callipyge region of ovine chromosome 18.
Bidwell, C. A.; Shay, T. L.; Georges, Michel ULg et al

in Animal Genetics (2001), 32(5), 248-56

The inheritance pattern of the skeletal muscle hypertrophy phenotype caused by the callipyge gene has been characterized as polar overdominance. We hypothesized that this trait may be caused by a gain or ... [more ▼]

The inheritance pattern of the skeletal muscle hypertrophy phenotype caused by the callipyge gene has been characterized as polar overdominance. We hypothesized that this trait may be caused by a gain or loss of gene expression because of the reversible nature of the phenotype in paternal vs. maternal inheritance. Suppression subtraction cDNA probes were made from skeletal muscle mRNA of normal (NN) and callipyge (C(Pat)N(Mat)) animals and hybridized to Southern blots containing bacterial artificial chromosomes (BACs) that comprise a physical contig of the callipyge region. The CN-NN probes hybridized to two ovine and seven bovine BACs. Sequence analysis of fragments within those BACs indicated short regions of similarity to mouse gene trap locus (gtl2). Northern blots analysis of RNA from hypertrophy-responsive muscles show a population of GTL2 mRNA centred around 2.4 kb that were abundantly expressed in 14-day prenatal NN and C(Pat)N(Mat) lambs but were down-regulated in day 14 and day 56 postnatal NN lambs. The expression of GTL2 remained elevated in 14- and 56-day-old C(Pat)N(Mat) lambs as well as in 56-day-old N(Pat)C(Mat) and CC lambs. Expression of GTL2 in the supraspinatus, which does not undergo hypertrophy, was very low for all genotypes and ages. Isolation of cDNA sequences show extensive alternative splicing and a lack of codon bias suggesting that GTL2 does not encode a protein. The mutation of the callipyge allele has altered postnatal expression of GTL2 in muscles that undergo hypertrophy and will help identify mechanisms involved in growth, genomic imprinting and polar overdominance. [less ▲]

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailMobilisation au fil de l'eau : dix années d'expérience de contrat de rivière en Wallonie (Belgique)
Rosillon, Françis ULg; Vander Borght, Paul ULg

in Annales des mines (2001)

Restaurer la rivière et les ressources en eau mais surtout amorcer une dynamique nouvelle et des comportements plus responsables, tels étaient les objectifs du contrat de rivière. Le bilan est aujourd’hui ... [more ▼]

Restaurer la rivière et les ressources en eau mais surtout amorcer une dynamique nouvelle et des comportements plus responsables, tels étaient les objectifs du contrat de rivière. Le bilan est aujourd’hui largement positif et l’expérience pourrait être partagée avec d’autres pays européens. Même si le modèle reste encore largement perfectible. [less ▲]

Detailed reference viewed: 25 (10 ULg)
Full Text
Peer Reviewed
See detailDay-by-day maturation of the long-term expression of cocaine sensitization acquired before weaning in the rat
Tirelli, Ezio ULg

in Behavioral Neuroscience (2001), 115(5), 1101-1110

This study aimed to identify the ontogenetic period during which long-term expression of behavioral sensitization to cocaine begins to emerge. Rat pups aged 4, 8, 12, or 16 days received a pretreatment of ... [more ▼]

This study aimed to identify the ontogenetic period during which long-term expression of behavioral sensitization to cocaine begins to emerge. Rat pups aged 4, 8, 12, or 16 days received a pretreatment of 4 daily injections of 15 mg/kg sc cocaine paired with the test chamber for 45 min. Pups were then tested for sensitization in that context after abstinence intervals ranging from 2 to 10 days. On test days, pups were videotaped, and their behavior was scored later. Sensitization was detected after intervals of 2, 4, 5, or 9 days in pups aged 4-7, 8-11, 12-15, or 16-19 days during pretreatment, respectively. These results suggest that the mechanisms for long-term retention of sensitization mature incrementally in the rat, starting to emerge gradually after the I st week of age, whereas those relevant to short-term retention and initiation of sensitization are present earlier. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailReduced dopamine function in depressed patients is related to suicidal behavior but not its lethality
Pitchot, William ULg; Adam, Martine ULg; Gonzalez Moreno, A. et al

in Psychoneuroendocrinology (2001), 26(7), 689-696

Several lines of evidence suggest a role for dopamine in the control of suicidal behaviour. Previously, we suggested an involvement of D2-dopaminergic function in the biology of suicide by demonstrating a ... [more ▼]

Several lines of evidence suggest a role for dopamine in the control of suicidal behaviour. Previously, we suggested an involvement of D2-dopaminergic function in the biology of suicide by demonstrating a smaller growth hormone (GH) response to apomorphine, a dopaminergic agonist, in depressed patients who later died by suicide. The purpose of the present study was to assess GH response to apomorphine in major depressed in-patients with a history of highly lethal suicide attempt compared to depressed patients with a low lethal lifetime suicide attempt history and non-attempters. The study was performed in a sample of 26 male depressed in-patients with a history of suicide attempt compared to 26 male depressed non-attempters. We observed a significant difference between suicide attempters and non-attempters (for GH peak, 6.3+/-5.1 ng/ml vs 15.8+/-14.2 ng/ml, F=10.3, df=1, 50, P=0.002). Moreover, GH peak responses to apomorphine did not differ between depressed patients with a high lethal lifetime suicide attempt history and patients who made low lethal lifetime suicide attempt. In conclusion, the results of the present study support a role for dopamine in the biology of suicidal behaviour. More specifically, an impaired GH response to apomorphine could be a marker of suicide risk. [less ▲]

Detailed reference viewed: 20 (1 ULg)
Peer Reviewed
See detailEffets indesirables cutanes des interferons
Paquet, Philippe ULg; Pierard-Franchimont, Claudine ULg; Arrese, J. E. et al

in Revue Médicale de Liège (2001), 56(10), 699-702

The alpha, beta and gamma recombinant interferons are indicated in a growing spectrum of therapeutic indications. Some unwanted side effects occur on the skin. The main clinical presentations include ... [more ▼]

The alpha, beta and gamma recombinant interferons are indicated in a growing spectrum of therapeutic indications. Some unwanted side effects occur on the skin. The main clinical presentations include vesiculo-bullous sometimes infiltrated dermatitis, vasculitis, necrosis, ulceration and alopecia. Exacerbation of dermatoses such as psoriasis is also possible. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailDetermination of a gravity and shock score for prognosis in equine surgical colic
Grulke, Sigrid ULg; Olle, E.; Detilleux, Johann ULg et al

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (2001), 48(8), 465-73

A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain ... [more ▼]

A retrospective study evaluated 200 surgical colic cases. A gravity score (GS) based on four clinical parameters estimating intestinal obstruction (rectal palpation, borborygmi, abdominal distension, pain) and classified into three categories was established and tested to determine if it could evaluate prognosis. A shock score (SS) based on six parameters was also attributed to each case. The overall survival rate was 54%. The statistical analysis showed a significant (P < 0.01) difference in the survival rate in the different categories of the GS, as well as in the categories of the SS. A multivariate logistic regression model showed that horses with GS 3 are 10.6 times more likely to die than those with GS 1. A model combining the two scores showed an odds ratio of 7.1 for GS 3 versus GS 1, and for SS 3 versus SS 1, the odds ratio was 7.2. [less ▲]

Detailed reference viewed: 41 (12 ULg)
Full Text
Peer Reviewed
See detail5',6'-Dehydroguiachrysine and 5',6'-Dehydroguiaflavine, Two Curarizing Quaternary Indole Alkaloids from the Stem Bark of Strychnos Guianensis
Penelle, J.; Christen, P.; Molgo, J. et al

in Phytochemistry (2001), 58(4), 619-26

Two new quaternary indole alkaloids 5',6'-dehydroguiachrysine (1) and 5',6'-dehydroguiaflavine (2) were isolated from Strychnos guianensis stem bark. Their structures were determined by analysis of ... [more ▼]

Two new quaternary indole alkaloids 5',6'-dehydroguiachrysine (1) and 5',6'-dehydroguiaflavine (2) were isolated from Strychnos guianensis stem bark. Their structures were determined by analysis of spectral data. Their inhibitory effects on neuromuscular transmission are also reported and compared to that of other quaternary alkaloids. [less ▲]

Detailed reference viewed: 17 (3 ULg)
Full Text
Peer Reviewed
See detailIsolation of Bovine Follicular Dendritic Cells Allows the Demonstration of a Particular Cellular Prion Protein
Thielen, Caroline ULg; Mélot, France ULg; Jolois, Olivier ULg et al

in Cell & Tissue Research (2001), 306(1), 49-55

As interaction of cellular prion protein (PrPc) and the infectious agent (PrPres) appears to be a crucial pathogenic step promoted by homology, variation in PrPc isoforms on bovine immune cells may ... [more ▼]

As interaction of cellular prion protein (PrPc) and the infectious agent (PrPres) appears to be a crucial pathogenic step promoted by homology, variation in PrPc isoforms on bovine immune cells may explain the absence of infectivity in most bovine lymph organs. In this study, we examined PrPc expression in bovine lymph organs (tonsils and lymph nodes) and on isolated follicular dendritic cells (FDCs). We used a panel of different monoclonal antibodies (MoAbs) raised against different epitopes of prion protein. Two MoAbs recognise amino acids 79-92 (SAF 34 and SAF 32 Mo-Abs); the 6H4 antibody reacts with a specific peptide comprising the 144-152 amino acids, and the 12F10 MoAb recognises the sequence 142-160. After immunolabelling of frozen sections of lymph organs with 6H4 or 12F10 MoAbs, we detected cellular prion protein in germinal centres. However, using the SAF 34 or SAF 32 antibodies, PrPc was revealed outside the lymphoid tissues. No PrPc was observed in the germinal centres. Therefore, we adapted the method of FDC isolation, making it suitable for the study of PrPc expression on their surface. Using electron microscopy, the presence of PrPc on the surface of FDCs was demonstrated only with 6H4 MoAb. These results suggest that bovine follicular dendritic cells express a particular form of prion protein. Either the N-terminal part of PrPc is cleaved or the accessibility of the specific epitope (79-92) of SAF 34 MoAb is abolished by interaction with other molecules. This particular isoform of PrPc on bovine FDCs might be related to the apparent absence of infectivity in lymph organs in cattle affected by bovine spongiform encephalopathy. [less ▲]

Detailed reference viewed: 48 (9 ULg)
Full Text
Peer Reviewed
See detailEtude pilote pour le developpement d'un registre de l'infarctus du myocarde en region liegeoise (Belgique)
Paul, I.; Bilge, A.; Bolly, F. et al

in Revue d'Epidémiologie et de Santé Publique (2001), 49(5), 423-9

BACKGROUND: In 1998, a permanent registry of myocardial infarction was developed in the Liege area (Belgium) to provide updated, exhaustive and validated data on the morbidity and mortality from ... [more ▼]

BACKGROUND: In 1998, a permanent registry of myocardial infarction was developed in the Liege area (Belgium) to provide updated, exhaustive and validated data on the morbidity and mortality from cardiovascular causes, to define the patients' profile, to identify myocardial infarction therapeutic strategies and to complete and make comparisons with data collected in other parts of the country through methodologically identical registers. METHODS: All acute coronary events lethal or non lethal among individuals from both genders aged from 25 to 69 years and living in the area were registered according to the methodology developed for the MONICA project (Multinational Monitoring of Trends and Determinants in Cardiovascular Diseases) of WHO. The three main selected data sources were: death certificates, general practitioners and cardiologists, hospitals. The events were categorised according to symptoms, cardiac enzymes, electrocardiogram, history of chronic ischaemic heart disease and necropsy findings. RESULTS: The coronary-event rates were 283/100,000 in men and 102/100,000 in women. The case fatality rate, 28 days after the onset of the symptoms, was 30.6% for men and 36.2% for women, and 77.5% of deaths occurred in the first 24 hours after the onset of the symptoms. CONCLUSION: The development of a myocardial infarction register at a regional level requires the involvement of all health professionals dealing with that pathology. Such register has valuable public health interests, providing exhaustive and validated data on the pathology and its evolution as well as useful information for improving therapeutic strategies and developing adapted preventive measures. [less ▲]

Detailed reference viewed: 19 (7 ULg)
Full Text
Peer Reviewed
See detailUne volumineuse masse intestinale
Delbecque, Katty ULg; Meiers, I.; Hardy, N. et al

in Annales de Pathologie (2001), 21(5), 451-2

Detailed reference viewed: 50 (1 ULg)
Full Text
Peer Reviewed
See detailEffect of Nitrous Oxide on the Bispectral Index and the 95% Spectral Edge Frequency of the Electroencephalogram During Surgery
Hans, Pol ULg; Bonhomme, Vincent ULg; Benmansour, H. et al

in Anaesthesia (2001), 56(10), 999-1002

We studied the effect of nitrous oxide on the bispectral index and 95% spectral edge frequency of the electroencephalogram in 20 patients undergoing lumbar surgery under general anaesthesia combined with ... [more ▼]

We studied the effect of nitrous oxide on the bispectral index and 95% spectral edge frequency of the electroencephalogram in 20 patients undergoing lumbar surgery under general anaesthesia combined with epidural administration of 5 mg morphine. [less ▲]

Detailed reference viewed: 44 (4 ULg)
Full Text
Peer Reviewed
See detailDaytime 50 Hz Magnetic Field Exposure and Plasma Melatonin and Urinary 6-Sulfatoxymelatonin Concentration Profiles in Humans
Crasson, Marion ULg; Beckers, Véronique; Pequeux, Christel ULg et al

in Journal of Pineal Research (2001), 31(3), 234-41

Concern about the health effects of extremely low frequency (ELF) magnetic fields (MF) has been raised by epidemiological studies indicating an association between certain cancers and living near power ... [more ▼]

Concern about the health effects of extremely low frequency (ELF) magnetic fields (MF) has been raised by epidemiological studies indicating an association between certain cancers and living near power lines or working in high electric field environments. Alterations in pineal function have been proposed as a mechanism through which power-frequency MFs may interact with living organisms. A double blind laboratory study was performed to evaluate daytime exposure effects of 100 microT root mean square (rms) 50 Hz MF. Three head exposure sessions of 30 min each were performed: sham, continuous, and intermittent (15 s on/off cycles) MFs were presented to each subject in early or late afternoon (13:30 or 16:30 hr). Twenty-one healthy male volunteers (20-27 yr old) participated in these 3-weekly experimental conditions. Blood samples were drawn for serum melatonin measurement, hourly at night (from 20:00 to 07:00 hr) under controlled environmental conditions. Urinary excretion of 6-sulfatoxymelatonin (aMT6s), the main melatonin metabolite, was measured for a 17 hr period, by means of urine samples taken at 19:00 hr (14:00-19:00 hr "afternoon period"), 23:00 hr (19:00-23:00 hr "evening period"), and 07:00 hr, day 2 (23:00-07:00 hr day 2 "night-time period"). There were no significant differences in either plasma melatonin or in aMT6s excretion profiles in the three experimental conditions. However, a tendency for a smaller increase of night-time urinary aMT6s after continuous MF exposure was found (P=0.08) particularly in men with the lower excretion rate of aMT6s ("Low Group") (P=0.07). We conclude that this study does not indicate that daytime acute MF exposure influences either melatonin secretion or aMT6s excretion. Inter-individual differences in pineal production of melatonin, however, have to be taken into account in further studies. [less ▲]

Detailed reference viewed: 40 (3 ULg)
Full Text
Peer Reviewed
See detailAcute St-Segment Elevation in Neisseria Meningitis
Gach, Olivier ULg; LANCELLOTTI, Patrizio ULg; PIERARD, Luc ULg

in Acta Cardiologica (2001), 56(5), 327-9

Meningitis due to Neisseria meningitidis occurred in a young man. On admission, he was in septic shock and the electrocardiogram revealed convex upwards ST-segment elevation in inferior and lateral leads ... [more ▼]

Meningitis due to Neisseria meningitidis occurred in a young man. On admission, he was in septic shock and the electrocardiogram revealed convex upwards ST-segment elevation in inferior and lateral leads. Rapid and complete normalisation of the ECG was observed and the patient had a favourable evolution with intensive therapy.The mechanisms of the ECG abnormalities in this disease are unclear. In this patient, ST-segment elevation was probably related to severe transmural ischaemia or prolonged coronary artery spasm as suggested by increase and decrease of cardiac enzymes and transient echocardiographic wall motion abnormalities without pericarditis. However, myocarditis could not be completely ruled out. [less ▲]

Detailed reference viewed: 23 (3 ULg)
Full Text
Peer Reviewed
See detailEvaluation of the Simple Calculated Osteoporosis Risk Estimation (Score) in a Sample of White Women from Belgium
Ben Sedrine, Wafa ULg; Devogelaer, J. P.; Kaufman, J. M. et al

in BONE (2001), 29(4), 374-80

Identifying patients at risk of developing an osteoporosis-related fracture will continue to be a challenge. The "gold standard" for osteoporosis diagnosis is bone densitometry. However, economic issues ... [more ▼]

Identifying patients at risk of developing an osteoporosis-related fracture will continue to be a challenge. The "gold standard" for osteoporosis diagnosis is bone densitometry. However, economic issues or availability of the technology may prevent its use under a mass screening scenario. A risk assessment instrument, the "simple calculated osteoporosis risk estimation" (SCORE), has been reported to appropriately identify women likely to have low (t score < or = -2 SD) bone mineral density (BMD) and who should be referred for bone densitometry. The aim of our study is to evaluate the discriminatory performance of SCORE in a random sample of postmenopausal white women from Belgium. For this purpose, we gathered medical data on 4035 consecutive patients aged > or = 45 years, either consulting spontaneously or referred for a BMD measurement to an outpatient osteoporosis center located at the University of Liege, Belgium. BMD measurements, using dual-energy X-ray absorptiometry (DXA) technology, were taken at the hip (total and neck) and lumbar spine (L2-4). At the recommended cutoff point of 6, SCORE had a sensitivity of 91.5% to detect low BMD at any of the measured sites, a specificity of 26.5%, a positive predictive value of 52.8%, and a negative predictive value of 77.7%. According to SCORE, 18% of the patients would not be recommended for densitometry. Among these, 10.9% were misclassified as they had osteoporosis (t score < or = -2.5 SD) at one or more of the sites investigated. The negative predictive errors of SCORE, when failing to detect osteoporosis, were only 1% for the total hip, 3.2% for the femoral neck, and 8.8% for the lumbar spine. We conclude that, notwithstanding the high values of sensitivity, SCORE specificity is too low to be useful as a diagnostic tool for screening patients at high risk to later develop osteoporosis. Nevertheless, from a resource allocation perspective, this instrument can be used with relative confidence to exclude patients who do not need a BMD measurement, and would therefore provide an opportunity to realize substantial cost savings in comparison to a mass screening strategy. [less ▲]

Detailed reference viewed: 208 (8 ULg)
Peer Reviewed
See detailLa bronchopneumopathie chronique obstructive (BPCO): concepts actuels
Louis, Renaud ULg

in Revue Médicale de Liège (2001), 56(10), 689-95

COPD is a widespread disease in western countries affecting 10% of adults and linked to tobacco consumption in 90% of cases. This disorder is characterized by a reduced maximal expiratory flow which is ... [more ▼]

COPD is a widespread disease in western countries affecting 10% of adults and linked to tobacco consumption in 90% of cases. This disorder is characterized by a reduced maximal expiratory flow which is irreversible and slowly progressive over the years. In those who are genetically prone to develop a COPD, chronic exposure to toxic fumes or particles generates a bronchiolitis and an emphysema which cause disturbances in ventilation mechanics and alveolar gaz exchange. This leads to symptoms of breathlessness, hypoxemia, pulmonary hypertension and, in end stage disease, cor pulmonale. The smoking cessation is the only way to prevent the occurrence of COPD. [less ▲]

Detailed reference viewed: 157 (22 ULg)
Peer Reviewed
See detailRipple and Kink Dynamics
Caps, Hervé ULg; Vandewalle, Nicolas ULg

in Physical Review. E : Statistical, Nonlinear, and Soft Matter Physics (2001), 64(4, Pt 1), 41302

We propose a relevant modification of the Nishimori-Ouchi model [Phys. Rev. Lett. 71, 197 (1993)] for granular landscape erosion. We explicitly introduce an additional parameter: the angle of repose ... [more ▼]

We propose a relevant modification of the Nishimori-Ouchi model [Phys. Rev. Lett. 71, 197 (1993)] for granular landscape erosion. We explicitly introduce an additional parameter: the angle of repose straight theta(r), and an additional process: avalanches. We show that the straight theta(r) parameter leads to an asymmetry of the ripples, as observed in natural patterns. The temporal evolution of the maximum ripple height h(max) is limited and not linear according to recent observations. The ripple symmetry and the kink dynamics are studied and discussed. [less ▲]

Detailed reference viewed: 21 (2 ULg)
Full Text
Peer Reviewed
See detailEtude clinique du mois. Rôle néphroprotecteur des antagonistes du récepteur de l'angiotensine II (ARA) dans le diabète de type 2: résultats des études IDNT et RENAAL
Weekers, Laurent ULg; Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (2001), 56(10), 723-726

Nephropathy associated with type 2 diabetes mellitus is a rising cause of end-stage renal disease and is a major public health problem. If blocking of the renin angiotensin system has a well established ... [more ▼]

Nephropathy associated with type 2 diabetes mellitus is a rising cause of end-stage renal disease and is a major public health problem. If blocking of the renin angiotensin system has a well established nephroprotective effect in type 1 diabetic nephropathy, this remained to be shown for type 2 diabetes. Two large outcome trials using angiotensin II receptor antagonists (ARA's) in proteinuric chronic renal impairment and hypertensive type 2 diabetic patients have now closed this gap: the Irbesartan Diabetic Nephropathy Trial (IDNT) and the Reduction of Endpoints in NIDDM with Angiotensin II Antagonist Losartan (RENAAL) trial. Both trials showed a significant reduction in the primary pre-specified end-point of death, or worsening of renal function (doubling of serum creatinine) or the development of end-stage renal disease. This effect goes beyond the reduction in blood pressure and makes of ARA's one of the important tools in the treatment of type 2 diabetic nephropathy. [less ▲]

Detailed reference viewed: 300 (1 ULg)
Peer Reviewed
See detailVasculite pustuleuse severe et polymorphe induite par le losartan
Pierard Franchimont, C.; Henry, Frédérique ULg; Pierard, Gérald ULg

in Annales de Dermatologie et de Vénéréologie (2001), 128(10, Pt 1), 1040-2

INTRODUCTION: We report a case of severe pustular vasculitis most probably due to losartan (Cozaar) which is an antihypertensive drug inhibiting the receptors for angiotensin II. CASE REPORT: A 64-year ... [more ▼]

INTRODUCTION: We report a case of severe pustular vasculitis most probably due to losartan (Cozaar) which is an antihypertensive drug inhibiting the receptors for angiotensin II. CASE REPORT: A 64-year-old woman, suffering from hypertension, developed a widespread and rapidly evolving bullous and pustular eruption without any other clinical sign. Leucocytoclastic vasculitis and junctional blisters were present with deposits of both IgA and IgG at these sites. Withdrawal of losartan, which was the only drug taken by the patient, and tapered corticotherapy cleared the lesions. The rash left a prominent mottled hyperpigmentation. It did not recur during the following months despite the presence of antineutrophil cytoplasmic antibodies. DISCUSSION: So far only a few cutaneous side effects have been reported following losartan intake. This drug was the likely culprit in the present case. Vasculitis was extremely severe and polymorphous. [less ▲]

Detailed reference viewed: 45 (0 ULg)
Peer Reviewed
See detailLe sens de la coherence et l'engagement dans un programme de revalidation cardiaque suite a un infarctus du myocarde: resultats preliminaires
Breuer, Bénédicte; Etienne, Anne-Marie ULg

in Revue Médicale de Liège (2001), 56(10), 703-708

This study investigate the impact of the sense of coherence or SOC (1) on the commitment to health behaviors, as the participation in a revalidation program (physical training) after a first myocardial ... [more ▼]

This study investigate the impact of the sense of coherence or SOC (1) on the commitment to health behaviors, as the participation in a revalidation program (physical training) after a first myocardial infarction. Fifteen subjects have been assessed three times after their myocardial infarction. The data indicate a possible effect of the sense of coherence which is higher among participants in comparison with subjects who refused to be involved in the program. [less ▲]

Detailed reference viewed: 133 (37 ULg)
Full Text
Peer Reviewed
See detailEtude de la variabilite interindividuelle de la variation contingente negative (VCN)
Crasson, Marion ULg; Lembreghts, M.; el Ahmadi, A. et al

in Neurophysiologie Clinique = Clinical Neurophysiology (2001), 31(5), 300-20

The use of CNV in clinical practice requires the choice of a standardised protocol, the constitution of reference normative data and the consideration of intra- and interindividual variability. For this ... [more ▼]

The use of CNV in clinical practice requires the choice of a standardised protocol, the constitution of reference normative data and the consideration of intra- and interindividual variability. For this purpose, we recorded CNV in 86 control subjects (44 men and 42 women, 18 to 62 years old (mean age = 34 +/- 13 years) during a reaction time paradigm with a warning signal and a 1-second S1-S2 interval. Moreover, the role of inter-stimulation interval was analysed in a group of 12 subjects through the comparison of recordings made with 1- and 3-second intervals. The CNV amplitude, its morphology and topographic distribution as well as its resolution mode and evolution through the recording were studied. The subjects' performances and their interactions with electroencephalographic data were also included in the analyses. Our results underscore the contribution of age and gender and psychological factors to CNV variability. CNV amplitude (both M1 and M2) increased and changed topographic distribution toward more central sites in older. Men had faster reaction times than women and lower post-S1 P300. Moreover, the life events-related stress and the subject's current anxiety level were accompanied by a decreased CNV amplitude. [less ▲]

Detailed reference viewed: 100 (1 ULg)
Full Text
Peer Reviewed
See detailOxidative Processes in Human Promonocytic Cells (Thp-1) after Differentiation into Macrophages by Incubation with Chlamydia Pneumoniae Extracts
Mouithys-Mickalad, Ange ULg; Deby-Dupont, Ginette; Nys, Monique ULg et al

in Biochemical and Biophysical Research Communications (2001), 287(3), 781-8

Human monocytes differentiated into macrophages by Chlamydia pneumoniae were able to oxidize blood lipoproteins, as discovered by Kalayoglu et al. (1998). Using a model of human promonocytic cells (THP-1 ... [more ▼]

Human monocytes differentiated into macrophages by Chlamydia pneumoniae were able to oxidize blood lipoproteins, as discovered by Kalayoglu et al. (1998). Using a model of human promonocytic cells (THP-1), the cells were differentiated into macrophages by preincubation with C. pneumoniae extract, and further stimulated by phorbol myristate acetate. In these conditions, the differentiated cells oxidized a thiol compound and released superoxide anion as demonstrated respectively by gas liquid chromatography and electron spin resonance. The thiol oxidation and superoxide anion release were inhibited by diphenyliodonium, a NADPH oxidase and NOsynthase inhibitor, proving that the respiratory burst and the NOsynthase were involved in the oxidation processes occurring in the differentiated THP-1. The role of H(2)O(2) (derived from superoxide anion) was indicated by the enhancing effect of a peroxidase on the thiol oxidation. The presence of alpha-tocopherol in the surrounding medium strongly diminished the oxidation of the thiol target. [less ▲]

Detailed reference viewed: 41 (7 ULg)
Full Text
Peer Reviewed
See detailEstablishment of Latency Associated with Glycoprotein E (Ge) Seroconversion after Bovine Herpesvirus 1 Infection in Calves with High Levels of Passive Antibodies Lacking Ge Antibodies
Schynts, F.; Lemaire, Mylène; Ros, C. et al

in Veterinary Microbiology (2001), 82(3), 211-22

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels ... [more ▼]

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels of passive immunity from cows repeatedly vaccinated with gE deleted marker vaccine. Four out of the six calves developed gE antibodies 3-5 weeks after infection, whereas the two other ones remained seronegative to gE. After 5 months of infection, the six calves were treated with dexamethasone. Virus was only re-excreted by the four calves which previously seroconverted against gE. The two other calves became seronegative against BHV-1, 30-32 weeks after infection. A second dexamethasone treatment performed 11 months after infection failed to demonstrate a latent infection in these two calves. Moreover, the lack of identification of a cell-mediated immune response, after the two dexamethasone treatments, and the failure to detect BHV-1 DNA sequences in trigeminal ganglia strongly suggest that these two calves were not latently infected. In conclusion, the presence of high levels of maternal immunity lacking gE antibodies does not prevent latency after infection with a low titre of BHV-1. Moreover, latency is associated with a serological response to gE. These results confirm that the gE deletion is a good marker to identify young calves latently infected with a field virus. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailInduction of Sparc by Vegf in Human Vascular Endothelial Cells
Kato, Y.; Lewalle, J. M.; Baba, Y. et al

in Biochemical and Biophysical Research Communications (2001), 287(2), 422-6

SPARC/osteonectin/BM-40 is a matricellular protein that is thought to be involved in angiogenesis and endothelial barrier function. Previously, we have detected high levels of SPARC expression in ... [more ▼]

SPARC/osteonectin/BM-40 is a matricellular protein that is thought to be involved in angiogenesis and endothelial barrier function. Previously, we have detected high levels of SPARC expression in endothelial cells (ECs) adjacent to carcinomas of kidney and tongue. Although SPARC-derived peptide showed an angiogenic effect, intact SPARC itself inhibited the mitogenic activity of vascular endothelial growth factor (VEGF) for ECs by the inhibiting phosphorylation of flt-1 (VEGF receptor 1) and subsequent ERK activation. Thus, the role of SPARC in tumor angiogenesis, stimulation or inhibition, is still unclear. To clarify the role of SPARC in tumor growth and progression, we determined the effect of VEGF on the expression of SPARC in human microvascular EC line, HMEC-1, and human umbilical vein ECs. VEGF increased the levels of SPARC protein and steady-state levels of SPARC mRNA in serum-starved HMEC-1 cells. Inhibitors (SB202190 and SB203580) of p38, a mitogen-activated protein (MAP) kinase, attenuated VEGF-stimulated SPARC production in ECs. Since intact SPARC inhibits phosphorylation ERK MAP kinase in VEGF signaling, it was suggested that SPARC plays a dual role in the VEGF functions, tumor angiogenesis, and extravasation of tumors mediated by the increased permeability of endothelial barrier function. [less ▲]

Detailed reference viewed: 32 (5 ULg)
Full Text
Peer Reviewed
See detailLatency and Reactivation of a Glycoprotein E Negative Bovine Herpesvirus Type 1 Vaccine: Influence of Virus Load and Effect of Specific Maternal Antibodies
Lemaire, Mylène; Schynts, Frédéric; Meyer, Gilles et al

in Vaccine (2001), 19(32), 4795-804

The effects of the vaccination of neonatal calves with a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV-1) were investigated in naive and passively immunised calves either with the ... [more ▼]

The effects of the vaccination of neonatal calves with a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV-1) were investigated in naive and passively immunised calves either with the recommended dose or a 5-fold concentrated one. After inoculation (PI), all calves excreted the virus vaccine except three passively immunised calves inoculated with the lower titre. No antibody response could be detected in passively immunised calves, whatever the dose used, and they all became BHV-1 seronegative and remained so after dexamethasone treatment (PDT). Nevertheless, as shown by a gamma-interferon assay, all calves that excreted the vaccine PI developed a cell-mediated immune response and a booster response was observed PDT, suggesting viral reactivation. The vaccine virus was recovered PDT from nasal secretions in two calves and BHV-1 DNA were detected in trigeminal ganglia from five calves belonging to all inoculated groups. The results show that the BHV-1 gE-negative vaccine can establish latency not only in naive but also in passively immunised neonatal calves after a single intranasal inoculation. Moreover, this study shows for the first time that the gE-negative vaccine, when used in passively immunised calves, can lead to seronegative vaccine virus carriers. [less ▲]

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailHemodiafiltration does not improve haemodynamics in a porcine model of endotoxin shock.
LAMBERMONT, Bernard ULg; Moonen, Martial; KOLH, Philippe ULg et al

in Intensive Care Medicine (2001), 27(2),

Detailed reference viewed: 30 (4 ULg)
Full Text
Peer Reviewed
See detailOptical spectroscopy of XMEGA targets in the Carina Nebula - III. The multiple system Tr 16-104 (â ¡CPD -59° 2603)
Rauw, Grégor ULg; Sana, H.; Antokhin, I. I. et al

in Monthly Notices of the Royal Astronomical Society (2001), 326

We discuss the orbital elements of the multiple system Tr 16-104 which is usually believed to be a member of the open cluster Trumpler 16 in the Carina complex. We show that Tr 16-104 could be a ... [more ▼]

We discuss the orbital elements of the multiple system Tr 16-104 which is usually believed to be a member of the open cluster Trumpler 16 in the Carina complex. We show that Tr 16-104 could be a hierarchical triple system consisting of a short-period (2.15d) eclipsing O7V+O9.5V binary bound to a B0.2 IV star. Our preliminary orbital solution of the third body indicates that the B star most probably describes an eccentric orbit with a period of ~285 or ~1341d around the close binary. Folding photometric data from the literature with our new ephemerides, we find that the light curve of the close binary exhibits rather narrow eclipses indicating that the two O stars must be well inside their Roche lobes. Our analysis of the photometric data yields a lower limit on the inclination of the orbit of the close binary of i>=77°. The stellar radii and luminosities of the O7 V and O9.5 V stars are significantly smaller than expected for stars of this spectral type. Our results suggest that Tr 16-104 lies at a distance of the order of 2.5kpc and support a fainter absolute magnitude for zero-age main-sequence O stars than usually adopted. We find that the dynamical configuration of Tr 16-104 corresponds to a hierarchical system that should remain stable provided that it suffers no strong perturbation. Finally, we also report long-term temporal variations of high-velocity interstellar Caii absorptions in the line of sight towards Tr 16-104. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailPlasticity of rat central inhibitory synapses through GABA metabolism
Engel, Dominique ULg; Pahner, I; Schulze, K et al

in Journal of Physiology (2001), 535(2), 473-82

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailImpaired Accumulation of Granulocytes in the Lung During Ozone Adaptation
Fievez, Laurence ULg; Kirschvink, N.; Dogne, S. et al

in Free Radical Biology & Medicine (2001), 31(5), 633-641

Respiratory alterations induced by an acute exposure to ozone (O(3)) paradoxically resolve during multiday exposure. This adaptation is characteristically accompanied by a gradual attenuation of lung ... [more ▼]

Respiratory alterations induced by an acute exposure to ozone (O(3)) paradoxically resolve during multiday exposure. This adaptation is characteristically accompanied by a gradual attenuation of lung neutrophilia. As maintenance of neutrophilia at the site of inflammation is due to cytokine-mediated delayed neutrophil apoptosis, which is associated with reduced levels of Bax, a proapoptotic protein, we sought to determine whether defects in these mechanisms could account for O(3) adaptation. Lung granulocytes obtained at different time points from calves exposed to 0.75 ppm O(3) for 12 h/d for 7 consecutive days neither showed enhancement of survival nor Bax deficiency, when compared to blood granulocytes. To further investigate the effects of an exogenous oxidative stress on neutrophil survival, human granulocytes were treated with hydrogen peroxide alone, or in combination with granulocyte/macrophage colony-stimulating factor, an antiapoptotic cytokine. Both treatments led to rapid apoptosis associated with downregulation of Bcl-x(L) and Bcl-2, two antiapoptotic proteins. This study shows that O(3) adaptation is associated with a failure in the mechanisms leading to accumulation of neutrophils at the site of inflammation, and suggests that this defect is due to direct proapoptotic effects of exogenous oxidative stress on granulocytes [less ▲]

Detailed reference viewed: 22 (3 ULg)
Full Text
Peer Reviewed
See detailS Phase Dependence and Involvement of Nf-Kappab Activating Kinase to Nf-Kappab Activation by Camptothecin
Habraken, Yvette ULg; Piret, Bernard; Piette, Jacques ULg

in Biochemical Pharmacology (2001), 62(5), 603-16

Camptothecin (CPT) and derivatives are topoisomerase I poisons currently used as anticancer drugs. Their cytotoxicity is maximal for cells in S phase. Using asynchronous and S phase-synchronized HeLa ... [more ▼]

Camptothecin (CPT) and derivatives are topoisomerase I poisons currently used as anticancer drugs. Their cytotoxicity is maximal for cells in S phase. Using asynchronous and S phase-synchronized HeLa cells, we showed that both the nuclear factor-kappaB (NF-kappaB) activation and its transcriptional activity, induced by CPT treatment, are enhanced in S phase cells. After CPT treatment, NF-kappaB activation reached a maximum within 2-3 hr and was still detectable after 24 hr. The nature of the complex evolved with time, forming mostly p50/p65 after 2 hr to almost exclusively p52 after 24 hr. In HeLa cells, the different steps of the induction were readily observable in S phase synchronized cells, whereas they were barely noticeable in a randomly growing cell population. The signal progressed through the activation of the IKK complex, the phosphorylation of IkappaBalpha, and the degradation of phosphorylated-IkappaBalpha and -IkappaBbeta. The stable expression of wild-type HA-tagged-IkappaBalpha or mutated HA-tagged-IkappaBalpha (S32,36A) allowed us to confirm the essential role of Ser32 and Ser36. NF-kappaB-activating kinase (NIK) could play a role upstream of the IKK complex, as the transient expression of a kinase inactive mutant NIK(K429,430A) abolished the activation of NF-kappaB by CPT. A kinase inactive mutant of mitogen-activated protein/ERK kinase kinase 1 (MEKK1), another kinase susceptible of acting upstream of the signalsome, did not. Cytotoxicity studies with clonal populations expressing different amounts of wild-type or mutated IkappaBalpha revealed that the overexpression of wild-type IkappaBa in large amount increases the sensitivity of HeLa cells to CPT more efficiently than a lower level of expression of non-phosphorylable IkappaBalpha. [less ▲]

Detailed reference viewed: 33 (5 ULg)
Full Text
Peer Reviewed
See detailOptical spectroscopy of X-Mega targets - II. The massive double-lined O-type binary HD 93205
Morrell, N. I.; Barbá, R. H.; Niemela, V. S. et al

in Monthly Notices of the Royal Astronomical Society (2001), 326

A new high-quality set of orbital parameters for the O-type spectroscopic binary HD 93205 has been obtained combining échelle and coudé CCD observations. The radial velocity orbits derived from the Heii ... [more ▼]

A new high-quality set of orbital parameters for the O-type spectroscopic binary HD 93205 has been obtained combining échelle and coudé CCD observations. The radial velocity orbits derived from the Heii lambda4686Å (primary component) and Hei lambda4471Å (secondary component) absorption lines yield semi-amplitudes of 133+/-2 and 314+/-2kms[SUP]-1[/SUP] for each binary component, resulting in minimum masses of 31 and 13M[SUB]solar[/SUB] (q=0.42). We also confirm for the binary components the spectral classification of O3V+O8V previously assigned. Assuming for the O8V component a `normal' mass of 22-25M[SUB]solar[/SUB] we would derive for the primary O3V a mass of `only' 52-60M[SUB]solar[/SUB] and an inclination of about 55° for the orbital plane. We have also determined for the first time a period of apsidal motion for this system, namely 185+/-16yr using all available radial velocity data sets of HD 93205 (from 1975 to 1999). Phase-locked variations of the X-ray emission of HD 93205 consisting of a rise of the observed X-ray flux near periastron passage are also discussed. [less ▲]

Detailed reference viewed: 27 (3 ULg)
Peer Reviewed
See detailTransfer of drospirenone to breast milk after a single oral administration of 3 mg drospirenone +30 mu g ethinylestradiol to healthy lactating women
Blode, H.; Foidart, Jean-Michel ULg; Heithecker, R.

in European Journal of Contraception & Reproductive Health Care (2001), 6(3), 167-171

Drospirenone (DPSP) is a synthetic progestogen which has been developed in combination with ethinylestradiol (EE) for use as an oral contraceptive (Yasmin(R), Schering AG, Berlin, Germany). The ... [more ▼]

Drospirenone (DPSP) is a synthetic progestogen which has been developed in combination with ethinylestradiol (EE) for use as an oral contraceptive (Yasmin(R), Schering AG, Berlin, Germany). The pharmacokinetic characteristics ofDRSP were evaluated in serum and breast milk from lactating women who received a single oral dose of 3 mg DRSP + 30 mug EE, to determine the fraction of the dose of DRSP which transfers to breast milk. Nine healthy, lactating women were included into the present study and pharmacokinetic data were obtained from six participants. The maximum DRSP concentrations (data given as mean standard deviation) were reached on average 2.5 +/- 1.2 and 2.8 +/- 1.3 h in serum and breast milk, respectively after oral administration of 3 mg DRSP + 30 mug EE, and amounted on average to 30.8 +/- 14.4 and 13.5 +/- 11.7 ng DRSP/ml in serum and breast milk. The mean breast milk versus serum concentration ratios of DPSP increased from 0.16 to 0.57 within 2 h after dosing and decreased to 0.16 after 24 h. The average ratio of AUC(0-48 h) , values in breast milk versus serum was 0.23 +/- 0.09. The mean DRSP concentration in breast milk over the 24-h period after dosing was 3.7 +/- 1.9 ng/ml. The amount of DRSP measured to be transferred into breast milk in the six women participating in the present study was, on average, 635 ng (range 256.2-1357.9 ng) within 24 h, corresponding to about 0.02% of the maternal dose. Based on the average concentration of the drug in breast milk over 24 h and assuming a daily ingestion of approximately 800 ml breast milk, the daily dose that reaches an infant via breast milk is estimated to be approximately 3 mug DPSP. The subjective and objective tolerances of 3 mg DRSP + 30 mug EE were good, with no adverse events reported. [less ▲]

Detailed reference viewed: 41 (1 ULg)
Full Text
Peer Reviewed
See detailIonic end-capping of (semi)telechelic polymers by mesogens: a novel route to liquid crystalline polymers
Gohy, Jean-François; Jérôme, Robert ULg

in Progress in Polymer Science (2001), 26(7), 1061-1099

A mesogenic cation has been associated with low molecular weight carboxylato- and sulfonato-(semi)telechelic polymers to form liquid crystalline halato(semi)telechelic polymers (LC H(S)TPs). Two methods ... [more ▼]

A mesogenic cation has been associated with low molecular weight carboxylato- and sulfonato-(semi)telechelic polymers to form liquid crystalline halato(semi)telechelic polymers (LC H(S)TPs). Two methods have been used to end-cap the linear polymer chains at one (or both) end(s) by a mesogen through ionic bonding. The first method relies on the ion-exchange reaction between the metal counterion of halato(semi)telechelic polymers and an ionic mesogen. The second method is based on the proton-transfer from a sulfonic or carboxylic acid end-group to a tertiary aliphatic amine, this approach being controlled by the relative pKa's of the acid and basic groups. The resulting materials have been characterized by differential scanning calorimetry (DSC), polarized optical microscopy (POM), small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS). On the basis of these experimental results, a model for the supramolecular organization of the LC H(S)TPs has been proposed. The final morphology results from the interplay of two competitive effects: the dipolar interaction of the ion-pairs known for multiplets formation and the propensity of the mesogenic moiety to form mesophases. The outcome of this competition depends on the mobility of the mesogenic counterion, i.e. on the strength of the dipolar interactions, the mobility of the polymer backbone and the mesogen/polymer ratio, which is controlled by the polymer molecular weight. A rod-like organization of the multiplets and a stretching of the polymer chains in the very close vicinity of the mesogenic core has been found in sulfonate polystyrenes, in agreement with the Eisenberg, Hird and Moore model for ionomers. The thickness of this region of restricted mobility has been estimated to 1 nm, which is the order of magnitude of the persistence length of polystyrene. Finally, LC H(S)TPs have been tested as interfacial agents in polystyrene/liquid crystal dispersions. The dipolar interactions of the ion pairs are clearly favorable to the additive localization at the polymer/LC interface. The higher polarity of the ammonium sulfonate pairs compared to the parent ammonium carboxylate ion pairs accordingly accounts for a higher interfacial activity. [less ▲]

Detailed reference viewed: 74 (1 ULg)
Full Text
Peer Reviewed
See detailDietary Polyamines and Non-Neoplastic Growth and Disease
Deloyer, Patricia; Peulen, Olivier ULg; Dandrifosse, Guy ULg

in European Journal of Gastroenterology & Hepatology (2001), 13(9), 1027-32

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have ... [more ▼]

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have a potential role in growth and development of the digestive system in neonatal mammals (and fishes). In humans, this property could be of importance in preventing the appearance of food allergies. Dietary polyamines also seem necessary for the maintenance of normal growth and general properties of adult digestive tract. Their possible therapeutic effects have been investigated in gastric, intestinal, and, more recently, whole-body healing. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Peer Reviewed
See detailL'image du mois. Tumeurs et ulcerations en rouge et blanc
Goffin, Véronique ULg; Hermanns-Lê, Trinh ULg; Pierard, Gérald ULg

in Revue Médicale de Liège (2001), 56(9), 597-8

Detailed reference viewed: 6 (1 ULg)
Peer Reviewed
See detailLe cas clinique du mois. A propos d'un cas d'hypertension portopulmonaire
Blaise, Pierre ULg; Louis, Renaud ULg; Quaden, C. et al

in Revue Médicale de Liège (2001), 56(9), 607-11

A case of portopulmonary hypertension discovered during exploration of an atypical faint is described and the pathophysiology and management are discussed.

Detailed reference viewed: 25 (0 ULg)
Peer Reviewed
See detailStructure of the Telomeric Ends of Mt DNA, Transcriptional Analysis and Complex I Assembly in the Dum24 Mitochondrial Mutant of Chlamydomonas Reinhardtii
Duby, Franceline ULg; Cardol, Pierre ULg; Matagne, René-Fernand ULg et al

in Molecular Genetics & Genomics (2001), 266(1), 109-14

The dum24 mutant of Chlamydomonas reinhardtii contains four types of altered mitochondrial linear genomes: two types of deleted monomers and two types of dimers resulting from fusions between some ... [more ▼]

The dum24 mutant of Chlamydomonas reinhardtii contains four types of altered mitochondrial linear genomes: two types of deleted monomers and two types of dimers resulting from fusions between some monomers via their deleted ends. All molecules lack at least cob, nd4 and the 3' end of nd5, three adjacent genes located in the left part of the genome. We present evidence showing that in dum24, as in other deletion mutants, the deletions extend to the left telomeric end, and propose that the only replicative forms in the mutants are the dimeric DNA molecules that possess intact telomeric structures at both ends. Two abnormally large transcripts produced from chimeric genes are detected in dum24, which throws some light on the location of potential promoter sequences and processing signals in the mitochondrial genome. Using BN-PAGE analysis and immunological methods to detect complex I, we further show that dum24 mitochondria do not possess the normal multimeric complex I (850 kDa), but produce a smaller, partially assembled, complex (650 kDa), demonstrating a role for ND4 and/or ND5 subunits(s) in complex I assembly. [less ▲]

Detailed reference viewed: 31 (3 ULg)
Peer Reviewed
See detailA la recherche de la sagesse entre les defensines et les antiseptiques a la surface de la peau
Goffin, Véronique ULg; Pierard, Gérald ULg

in Revue Médicale de Liège (2001), 56(9), 635-8

The density in the natural bacterial flora is quite controlled at the skin surface. The barrier to their penetration inside the body is a major function of the stratum corneum. Its more or less ... [more ▼]

The density in the natural bacterial flora is quite controlled at the skin surface. The barrier to their penetration inside the body is a major function of the stratum corneum. Its more or less anfractuous structure, its hydration and acidic pH are important to maintain the microbiological equilibrium. The non-immune innate or inducible natural defenses rely on the production of epithelial peptide antibiotics (defensins, calprotectin) by keratinocytes. The phagocytic and immune defenses are superimposed to such mechanisms. This whole conundrum may be helped by, but it may also suffer from some skin care products and antiseptics. [less ▲]

Detailed reference viewed: 10 (2 ULg)
Peer Reviewed
See detailUltrasonographic examination of the palmar aspect of the fetlock and pastern: Technique and normal images
Busoni, Valeria ULg

in Ippologia (2001), 12(3), 5

In soft tissue injuries of the equine limbs, the ultrasonographic examination is essential to make a diagnosis, to establish an orthapaedic treatment and to suggest a prognosis. A good knowledge of ... [more ▼]

In soft tissue injuries of the equine limbs, the ultrasonographic examination is essential to make a diagnosis, to establish an orthapaedic treatment and to suggest a prognosis. A good knowledge of ultrasonographic anatomy is the base of musculo-skeletal ultrasonography: this paper presents the ultrasonographic anatomy of the palmar aspect of the pastern and fetlock regions and gives useful technical details to perform a complete ultrasonographic examination of these regions. The ultrasonographic anatomy is described and illustrated by transverse and longitudinal ultrasonographic Sections obtained with a 7.5 MHz linear transducer. [less ▲]

Detailed reference viewed: 75 (4 ULg)
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Syndrome carcinoïde sur tumeur sécrétante du grêle terminal avec cardiopathie
Compere, C.; Geronooz, Isabelle; Croes, F. et al

in Revue Médicale de Liège (2001), 56(9), 599-606

Uncommon but classically described, intestinal carcinoid tumors represent a variety of neuroendocrine tumors. Able to secrete amines and hormones, they can produce characteristic symptoms called ... [more ▼]

Uncommon but classically described, intestinal carcinoid tumors represent a variety of neuroendocrine tumors. Able to secrete amines and hormones, they can produce characteristic symptoms called "carcinoid syndrome". A cardiopathy is sometimes associated with the disease and carries a high risk of mortality and morbidity. [less ▲]

Detailed reference viewed: 351 (7 ULg)
Full Text
Peer Reviewed
See detailLe musée mélancolique. Tentatives pour photographier nos manières de voir
Havelange, Carl ULg

in publics et musées. Revue internationale de muséologie (2001), 1999 (paru en 2001)(16), 10-16

Detailed reference viewed: 25 (1 ULg)
Full Text
Peer Reviewed
See detailThe Penicillin Resistance of Enterococcus Faecalis Jh2-2r Results from an Overproduction of the Low-Affinity Penicillin-Binding Protein Pbp4 and Does Not Involve a Psr-Like Gene
Duez, Colette ULg; Zorzi, Willy ULg; Sapunaric, Frédéric ULg et al

in Microbiology (2001), 147(Pt 9), 2561-9

A penicillin-resistant mutant, JH2-2r (MIC 75 microg ml(-1)), was isolated from Enterococcus faecalis JH2-2 (MIC 5 microg ml(-1)) by successive passages on plates containing increasing concentrations of ... [more ▼]

A penicillin-resistant mutant, JH2-2r (MIC 75 microg ml(-1)), was isolated from Enterococcus faecalis JH2-2 (MIC 5 microg ml(-1)) by successive passages on plates containing increasing concentrations of benzylpenicillin. A comparison of the penicillin-binding protein (PBP) profiles in the two strains revealed a more intensely labelled PBP4 in JH2-2r. Because the sequences of the JH2-2 and JH2-2r pbp4 genes were strictly identical, even in their promoter regions, this intensive labelling could only be associated with an overproduction of the low-affinity PBP4. No psr gene analogous to that proposed to act as a regulator of PBP5 synthesis in Enterococcus hirae and Enterococcus faecium could be identified in the vicinity of pbp4 in E. faecalis JH2-2 and JH2-2r. However, a psr-like gene distant from pbp4 was identified. The cloning and sequencing of that psr-like gene from both E. faecalis strains indicated that they were identical. It is therefore postulated that the PBP4 overproduction in E. faecalis JH2-2r results from the modification of an as yet unidentified factor. [less ▲]

Detailed reference viewed: 106 (13 ULg)
Full Text
Peer Reviewed
See detailGeneration of rapid eye movements during paradoxical sleep in humans
Peigneux, Philippe ULg; Laureys, Steven ULg; Fuchs, Sonia et al

in NeuroImage (2001), 14(3), 701-708

Although rapid eye movements (REMs) are a prominent feature of paradoxical sleep (PS), their origin and functional significance remain poorly understood in humans. In animals, including nonhuman primates ... [more ▼]

Although rapid eye movements (REMs) are a prominent feature of paradoxical sleep (PS), their origin and functional significance remain poorly understood in humans. In animals, including nonhuman primates, REMs during PS are closely related to the occurrence of the so-called PGO waves, i.e., prominent phasic activities recorded throughout the brain but predominantly and most easily in the pons (P), the lateral geniculate bodies (G), and the occipital cortex (O). Therefore, and because the evolution of species is parsimonious, a plausible hypothesis would be that during PS in humans, REMs are generated by mechanisms similar to PGO waves. Using positron emission tomography and iterative cerebral blood flow measurements by H(2)(15)O infusions, we predicted that the brain regions where the PGO waves are the most easily recorded in animals would be differentially more active in PS than in wakefulness, in relation with the density of the REM production [i.e., we looked for the condition (PS versus wakefulness) by performance (REM density) interaction]. Accordingly, we found a significant interaction effect in the right geniculate body and in the primary occipital cortex. The result supports the hypothesis of the existence of processes similar to PGO waves in humans, responsible for REM generation. The interest in the presence of PGO waves in humans is outstanding because the cellular processes involved in, or triggered by, PGO waves might favor brain plasticity during PS. [less ▲]

Detailed reference viewed: 25 (2 ULg)
Full Text
Peer Reviewed
See detailA mutation in the GTPase domain of the large subunit rRNA is involved in the suppression of a -1T frameshift mutation affecting a mitochondrial gene in Chlamydomonas reinhardtii
Matagne, René-Fernand ULg; Baurain, Denis ULg

in Molecular Genetics & Genomics (2001), 266(1), 103-108

The dum19 mutation isolated in Chlamydomonas reinhardtii is due to the deletion of one T at codon 152 of the mitochondrial cox1 gene sequence. Phenotypically, the dum19 mutant is characterized by a lack ... [more ▼]

The dum19 mutation isolated in Chlamydomonas reinhardtii is due to the deletion of one T at codon 152 of the mitochondrial cox1 gene sequence. Phenotypically, the dum19 mutant is characterized by a lack of cytochrome c oxidase activity and is unable to grow under heterotrophic conditions. A spontaneous pseudo-revertant that grows slowly in the dark was isolated from the dum19 mutant strain. A genetic and molecular analysis allowed us to demonstrate that the revertant phenotype is the consequence of two additional mutations that together act as a frameshift suppressor: an m mutation affecting a mitochondrial gene other than cox1 and an n mutation affecting a nuclear gene. On its own the n mutation does not act as a suppressor, whereas the m mutation very slightly compensates for the effect of the -1T mutation. Sequencing analysis showed that the m mutation affects the GTPase-associated domain of the large subunit (LSU) ofmitochondrial rRNA. Surprisingly, two substitutions, A1090 to G and A1098 to C, were found in the LSU rRNA of the revertant, the latter one being already present in the dum19 mutant strain itself. The A1090 to G substitution is thus involved in the suppression of the frameshift mutation, but it is not clear whether the change at position 1098 is also required for the expression of the suppressed phenotype. To our knowledge, this is the first example of a mutation in the GTPase-associated domain acting as a suppressor of a frameshift mutation. [less ▲]

Detailed reference viewed: 9 (1 ULg)
See detailHistoire des RAYMOND RASBERRY SINGERS
Sacré, Robert ULg

in Soul Bag (2001), (160), 32-35

ASTRAL HARMONIES - Histoire des Raymond Rasberry Singers : Opal Louis Nations , Dr. Odie H. Tolbert Jr.

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailNecrotoxigenic Escherichia Coli Type-2 Invade and Cause Diarrhoea During Experimental Infection in Colostrum-Restricted Newborn Calves
Van Bost, Sigrid; Roels, S.; Mainil, Jacques ULg

in Veterinary Microbiology (2001), 81(4), 315-29

There exists experimental evidence that necrotoxigenic Escherichia coli (NTEC) strains producing the cytotoxic necrotising factor 1 cause intestinal and extra-intestinal disease in piglets. On the other ... [more ▼]

There exists experimental evidence that necrotoxigenic Escherichia coli (NTEC) strains producing the cytotoxic necrotising factor 1 cause intestinal and extra-intestinal disease in piglets. On the other hand, no experimental model has been developed with NTEC strains producing the cytotoxic necrotising factor 2. In all, 14 colostrum-restricted calves were orally challenged with two strains isolated from the faeces of a diarrheic calf (B20a) or from the heart blood of a septicaemic calf (1404). All calves had diarrhoea which lasted until euthanasia in eight of them. In those calves, diarrhoea was correlated with the faecal excretion of the challenge strains. At necropsy, vascular congestion of the intestinal mucosa, hypertrophy of the mesenteric lymph nodes (MLN) and some congestion of the lungs were observed. Bacteriology confirmed the colonisation of the intestine by the challenge strains which were also recovered from the heart blood, the lungs and/or the liver. Histological sections confirmed enterocolitis, lymphadenitis and limited bronchopneumonia. In the intestinal tissue sections, bacteria testing positive in an in situ DNA hybridisation assay with a CNF2 probe were observed. Those results were confirmed by immunohistochemistry with a polyclonal anti-O78 and a monoclonal anti-F17b antisera. Three of the five control calves receiving either saline or a CNF(-), F17a strain (25KH09) had no clinical signs or lesions. The other two presented a profuse liquid diarrhoea but those calves were positive for the presence of K99(+) E. coli. In this model, both NTEC2 strains were thus, able to colonise the intestine, to cause long-lasting diarrhoea and to invade the blood stream with localisation in various internal organs in colostrum-restricted conventional newborn calves. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailLiving ring-opening (co)polymerization of 6,7-dihydro-2(5H)-oxepinone into unsaturated aliphatic polyesters
Lou, Xudong; Detrembleur, Christophe ULg; Lecomte, Philippe ULg et al

in Macromolecules (2001), 34(17), 5806-5811

Homopolymerization of the unsaturated cyclic ester, 6,7-dihydro-2(5H)-oxepinone (DHO), and copolymerization with epsilon-caprolactone (epsilon-CL) are an easy way to produce unsaturated aliphatic ... [more ▼]

Homopolymerization of the unsaturated cyclic ester, 6,7-dihydro-2(5H)-oxepinone (DHO), and copolymerization with epsilon-caprolactone (epsilon-CL) are an easy way to produce unsaturated aliphatic polyesters in a controlled manner, Polymerization of DHO initiated by Al((OPr)-Pr-i)(3) in toluene at room temperature proceeds by a coordination-insertion mechanism and is living as certified by the agreement between the experimental molecular weight at total monomer conversion and the value predicted from the initial monomer to initiator molar ratio. The polydispersity is also low (Mw/Mn less than or equal to 1.2). Random copolymerization of DHO and epsilon-CL leads to the same conclusion. Beyond the complete monomer conversion, the poly(DHO) chains experience mainly intermolecular transesterification reactions at the same rate as poly(epsilon CL) under the same conditions. The livingness of the ROP of both DHO and epsilon-CL allows diblock copolymers to be synthesized with predicted composition and molecular weight. Homopoly(DHO) is semicrystalline with Tm at 35°C and Tg at -50°C. Tm and thermal stability can be modulated by copolymerization of DHO with epsilon-CL. [less ▲]

Detailed reference viewed: 54 (3 ULg)
Full Text
Peer Reviewed
See detailP65 Homodimer Activity in Distal Airway Cells Determines Lung Dysfunction in Equine Heaves
Sandersen, Charlotte ULg; Bureau, Fabrice ULg; Turlej, R. et al

in Veterinary Immunology and Immunopathology (2001), 80(3-4), 315-26

Nuclear factor-kappaB (NF-kappaB) activity, which is a key regulator of inflammatory gene expression, is increased in bronchial epithelial cells from horses suffering from heaves (a hypersensitivity ... [more ▼]

Nuclear factor-kappaB (NF-kappaB) activity, which is a key regulator of inflammatory gene expression, is increased in bronchial epithelial cells from horses suffering from heaves (a hypersensitivity-associated inflammatory condition of the lung). To determine whether this increased activity extends to distal airways and to other pulmonary cells, cells recovered by broncho-alveolar lavage (BAL) in healthy and heaves-affected horses were assessed for NF-kappaB activity. NF-kappaB activity was much higher in BAL cells from heaves-affected horses, especially during crisis (disease exacerbation), than in cells from healthy horses. Moreover, the level of NF-kappaB activity found in BAL cells was positively correlated to total lung resistance and to the proportion of neutrophils present in BAL fluid. Finally, prototypical p65-p50 NF-kappaB heterodimers were absent from BAL cells, which mostly contained p65 homodimers. These results (1) show that increased NF-kappaB activity is a general feature of heaves lung; (2) demonstrate the importance of p65 homodimers in neutrophilic inflammation; and (3) suggest that the use of specific NF-kappaB inhibitors could improve lung function in heaves-affected horses. [less ▲]

Detailed reference viewed: 25 (9 ULg)
Peer Reviewed
See detailComparison of Thiopentone/Guaifenesin, Ketamine/Guaifenesin and Ketamine/Midazolam for the Induction of Horses to Be Anaesthetised with Isoflurane
Gangl, M.; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2001), 149(5), 147-51

Forty-eight horses subjected to elective surgery were randomly assigned to three groups of 16 horses. After premedication with 0.1 mg/kg acepromazine intramuscularly and 0.6 mg/kg xylazine intravenously ... [more ▼]

Forty-eight horses subjected to elective surgery were randomly assigned to three groups of 16 horses. After premedication with 0.1 mg/kg acepromazine intramuscularly and 0.6 mg/kg xylazine intravenously, anaesthesia was induced either with 2 g thiopentone in 500 ml of a 10 per cent guaifenesin solution, given intravenously at a dose of 1 ml/kg (group TG), or with 100 mg/kg guaifenesin and 2.2 mg/kg ketamine given intravenously (group KG), or with 0.06 mg/kg midazolam, and 2.2 mg/kg ketamine given intravenously (group KM). Anaesthesia was maintained with isoflurane. The mean (sd) end tidal isoflurane concentration (per cent) needed to maintain a light surgical anaesthesia (stage III, plane 2) was significantly lower in group KM (0.91 [0.03]) than in groups TG (1.11 [0.03]) and KG (1.14 [0.03]). The mean (sd) arterial pressure (mmHg) was significantly lower in group KG (67.4 [2.07]) than in groups TC (75.6 [2.23]) and KM (81.0 [2.16]). There were no significant differences in the logarithm of the heart rate, recovery time or quality of recovery between the three induction groups. However, pronounced ataxia was observed in the horses of group KM, especially after periods of anaesthesia lasting less than 75 minutes. [less ▲]

Detailed reference viewed: 17 (4 ULg)
Full Text
Peer Reviewed
See detailFast Clean-up for Polychlorinated Dibenzo-P-Dioxins, Dibenzofurans and Coplanar Polychlorinated Biphenyls Analysis of High-Fat-Content Biological Samples
Focant, Jean-François ULg; Eppe, Gauthier ULg; Pirard, Catherine ULg et al

in Journal of Chromatography. A (2001), 925(1-2), 207-21

A fast clean-up procedure for the low level analysis of polychlorinated dibenzo-p-dioxins. polychlorinated dibenzofurans and coplanar polychlorinated biphenyls in highly fatty biological matrices using ... [more ▼]

A fast clean-up procedure for the low level analysis of polychlorinated dibenzo-p-dioxins. polychlorinated dibenzofurans and coplanar polychlorinated biphenyls in highly fatty biological matrices using high capacity disposable multi-layer silica columns is presented. Results were compared with gel permeation chromatography for removal of lipids. Analytical criteria such as recovery rates, repeatability, reproducibility and robustness are evaluated through a broad range of biological matrices and reference materials analysis. The final proposed procedure for the complete analysis, including pressurized liquid extraction, Power-Prep system clean-up and GC-high-resolution MS analysis requires only 48 h, and allows the simultaneous preparation of up to 10 samples. [less ▲]

Detailed reference viewed: 59 (19 ULg)
Full Text
Peer Reviewed
See detailInterstellar Bubbles in Two Young H II Regions
Nazé, Yaël ULg; Chu, You-Hua; Points, Sean D et al

in Astronomical Journal (The) (2001), 122

Massive stars are expected to produce wind-blown bubbles in the interstellar medium; however, ring nebulae, suggesting the existence of bubbles, are rarely seen around main-sequence O stars. To search for ... [more ▼]

Massive stars are expected to produce wind-blown bubbles in the interstellar medium; however, ring nebulae, suggesting the existence of bubbles, are rarely seen around main-sequence O stars. To search for wind-blown bubbles around main-sequence O stars, we have obtained high-resolution Hubble Space Telescope WFPC2 images and high-dispersion echelle spectra of two pristine H II regions, N11B and N180B, in the Large Magellanic Cloud. These H II regions are ionized by OB associations that still contain O3 stars, suggesting that the H II regions are young and have not hosted any supernova explosions. Our observations show that wind-blown bubbles in these H II regions can be detected kinematically, but not morphologically, because their expansion velocities are comparable to or only slightly higher than the isothermal sound velocity in the H II regions. Bubbles are detected around concentrations of massive stars, individual O stars, and even an evolved red supergiant (a fossil bubble). Comparisons between the observed bubble dynamics and model predictions show a large discrepancy (1-2 orders of magnitude) between the stellar wind luminosity derived from bubble observations and models and that derived from observations of stellar winds. The number and distribution of bubbles in N11B differ from those in N180B, which can be explained by the difference in the richness of stellar content between these two H II regions. Most of the bubbles observed in N11B and N180B show a blister structure, indicating that the stars were formed on the surfaces of dense clouds. Numerous small dust clouds, similar to Bok globules or elephant trunks, are detected in these H II regions, and at least one of them hosts on-going star formation. [less ▲]

Detailed reference viewed: 35 (0 ULg)
Full Text
Peer Reviewed
See detailKinetic Study of Two Novel Enantiomeric Tricyclic Beta-Lactams Which Efficiently Inactivate Class C Beta-Lactamases
Vilar, M.; Galleni, Moreno ULg; Solmajer, T. et al

in Antimicrobial Agents and Chemotherapy (2001), 45(8), 2215-23

A detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, Lek 156 and Lek 157, and representative beta-lactamases and D-alanyl-D-alanine peptidases (DD ... [more ▼]

A detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, Lek 156 and Lek 157, and representative beta-lactamases and D-alanyl-D-alanine peptidases (DD-peptidases) is presented. Both compounds are very efficient inactivators of the Enterobacter cloacae 908R beta-lactamase, which is usually resistant to inhibition. Preliminary experiments indicate that various extended-spectrum class C beta-lactamases (ACT-1, CMY-1, and MIR-1) are also inactivated. With the E. cloacae 908R enzyme, complete inactivation occurs with a second-order rate constant, k(2)/K', of 2 x 10(4) to 4 x 10(4) M(-1) s(-1), and reactivation is very slow, with a half-life of >1 h. Accordingly, Lek 157 significantly decreases the MIC of ampicillin for E. cloacae P99, a constitutive class C beta-lactamase overproducer. With the other serine beta-lactamases tested, the covalent adducts exhibit a wide range of stabilities, with half-lives ranging from long (>4 h with the TEM-1 class A enzyme), to medium (10 to 20 min with the OXA-10 class D enzyme), to short (0.2 to 0.4 s with the NmcA class A beta-lactamase). By contrast, both carbapenems behave as good substrates of the Bacillus cereus metallo-beta-lactamase (class B). The Streptomyces sp. strain R61 and K15 extracellular DD-peptidases exhibit low levels of sensitivity to both compounds. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailPositron Emission Tomography (PET) for Staging Low-Grade Non-Hodgkin's Lymphomas (NHL)
Najjar, F.; Hustinx, Roland ULg; Jerusalem, Guy ULg et al

in Cancer Biotherapy & Radiopharmaceuticals (2001), 16(4), 297-304

Although positron emission tomography (PET) imaging is now recognized as a useful tool for staging intermediate and high-grade non-Hodgkin's lymphoma (NHL), few data are available regarding its accuracy ... [more ▼]

Although positron emission tomography (PET) imaging is now recognized as a useful tool for staging intermediate and high-grade non-Hodgkin's lymphoma (NHL), few data are available regarding its accuracy in low grade NHL. We therefore studied 36 patients with histologically proven low-grade NHL. Whole-body 2-(fluorine-18) fluoro-2-deoxy-D-glucose (FDG) PET was performed at the time of initial diagnosis (n = 21) or for disease recurrence (n = 15) prior to any treatment. PET results were compared to those of physical examination and computed tomography (CT). PET studies were read without knowledge of any clinical data. Any focus of increased activity was described and given a probability of malignancy using a 5 point-scale (0: normal to 4: definitively malignant). An individual biopsy was available for a total of 31 lesions. The sensitivity and specificity were 87% and 100% for FDG-PET, 100% and 100% for physical examination and 90% and 100% for CT respectively. In addition, 42 of 97 peripheral lymph node lesions observed by FDG-PET were clinically undetected, whereas the physical examination detected 23 additional nodal lesions. PET and CT both indicated 12 extranodal lymphomatous localizations. FDG-PET showed 7 additional extranodal lesions while 5 additional unconfirmed lesions were observed on CT. Regarding bone marrow infiltration, PET and biopsy were concordant in 24 patients with 11 true positive (TP) and 13 true negative (TN). However PET was FN in 11 patients and no biopsy was performed in one patient. The combination PET/CT/physical examination seems to be more sensitive than the conventional approach for staging low grade NHL. Its sensitivity however is unacceptably low for diagnosing bone marrow infiltration. [less ▲]

Detailed reference viewed: 45 (2 ULg)
Peer Reviewed
See detailAnalytic Quantification of Phototype-Related Regional Skin Complexion
Hermanns, Jean-François ULg; Petit, L.; Hermanns-Lê, Trinh ULg et al

in Skin Research & Technology (2001), 7(3), 168-71

BACKGROUND/AIMS: Narrow-band spectrophotometry that yields melanin (M) and erythema (E) indexes is a convenient method for assessing skin colour. The objective of the study was to assess the phenotype ... [more ▼]

BACKGROUND/AIMS: Narrow-band spectrophotometry that yields melanin (M) and erythema (E) indexes is a convenient method for assessing skin colour. The objective of the study was to assess the phenotype-associated body site differences in skin complexion. METHOD: The Mexameter MX16 was used to record the skin colour in 137 subjects. Evaluations were made on the inner arm, volar forearm, dorsal forearm and forehead. RESULTS: The inner arm and volar forearm had similar values for each of the colorimetric values. The dorsal forearm exhibited the highest M values, and the forehead had the highest E values. Significant positive linear correlations were found between M values from the distinct body sites. The E values showed a correlation only between the inner arm and the volar forearm. CONCLUSION: Regional skin colour differences are obvious. Sun-exposed skin does not react in the same way on the forearm and forehead. Regional variations in M can be predicted from the innate M value. In contrast, the regional variability in E is highly variable among subjects. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailReversal of Chloroquine and Mefloquine Resistance in Plasmodium Falciparum by the Two Monoindole Alkaloids, Icajine and Isoretuline
Frederich, Michel ULg; Hayette, Marie-Pierre ULg; Tits, Monique ULg et al

in Planta Medica (2001), 67(6), 523-7

Eight naturally occurring monoindole alkaloids were evaluated in vitro for their ability to inhibit Plasmodium falciparum growth and, in drug combination, to reverse the resistance of a chloroquine ... [more ▼]

Eight naturally occurring monoindole alkaloids were evaluated in vitro for their ability to inhibit Plasmodium falciparum growth and, in drug combination, to reverse the resistance of a chloroquine-resistant strain of Plasmodium falciparum. None of these indole alkaloids has significant intrinsic antiplasmodial activity (IC(50) > 10 microM or 5 microg/ml). Nevertheless, three alkaloids (icajine, isoretuline and strychnobrasiline) did reverse chloroquine resistance at concentrations between 2.5 and 25 microg/ml (IF of 12.82 for isoretuline on W2 strain). The Interaction Factor (IF) equals 2, < 2, or > 2 for additive, antagonistic or synergistic effects of alkaloids on chloroquine inhibition, respectively. Icajine and isoretuline were also assessed in vitro for their mefloquine potentiating activity on a mefloquine-resistant strain of Plasmodium falciparum. Only icajine proved to be synergistic with mefloquine (IF = 15.38). [less ▲]

Detailed reference viewed: 51 (8 ULg)
Full Text
Peer Reviewed
See detailEnvironmental evaluation of materials and buildings processes : application of the life cycle analysis to the construction of an industrial hall
Courard, Luc ULg; Rademaker, C.; Teller, P.

in Materials and Structures (2001), 34

Methodologies based on Life Cycle Assesment (L.C.A) give the opportunity to realise a global and complete evaluation of the environmental effects of products from their production to their use and ... [more ▼]

Methodologies based on Life Cycle Assesment (L.C.A) give the opportunity to realise a global and complete evaluation of the environmental effects of products from their production to their use and elimination (from craddle to grave); methods used for analysis are internationally wellknown and standardised. These methods are rarely used in construction materials area, although materials flows and energy consumption are important and constructions have a long way of life. A calculation methodology, based on ecofactors/ecopoints, has been used for the analysis of industrial hall made of concrete, steel, or concrete/wood structure; materials but also construction process are compared at the point of view of their environmental impact. This analysis completes the technical and economical approaches for the construction owner and designer and gives a global view of the interaction between construction and civil engineering, and environment. [less ▲]

Detailed reference viewed: 67 (5 ULg)
Full Text
See detailAnalyse de 516 rapports de reaction apres transfusion de produits sanguins labiles
Frere, M.C.; Rapaille, A.; Bouillenne, C. et al

in Transfusion Clinique et Biologique (2001), 8(4), 333-42

BACKGROUND: In order to assess the implemented preventive measures of transfusion reactions (TR) and to make a study of residual reactions, we analyzed 516 TR reports from 14 hospitals, for three years ... [more ▼]

BACKGROUND: In order to assess the implemented preventive measures of transfusion reactions (TR) and to make a study of residual reactions, we analyzed 516 TR reports from 14 hospitals, for three years since 1996 to 1998. METHODS: Clinical signs were classified according to seven etiologic categories. Systematic anti-erythrocyte and anti-leucocyte detection, as well as bacterial control of the returned bag were performed. RESULTS: The TR incidence is 3.7 per 1.000 products. Platelet concentrates (PC) provoke 7.4 TR per 1.000 transfusions, and red cell concentrates (RCC) 3.8. There are as many TR with apheresis platelets (AP), pre-storage leuco-depleted, as with random platelets, post-storage leuco-depleted, and as many with leuco-depleted RCC as with non leuco-depleted RCC. Leuco-depleted AP provoke more allergic reactions than other blood components. TR with AP are much more frequent in children than in adults. Plasma removal from AP before transfusion decreases reaction frequency. CONCLUSIONS: The lack in efficacy failure of pre-storage deleucocytation in TR prevention should be due to related patient factors. Etiology of AP allergic reactions deserves further study. PC suspension in synthetic medium before transfusion is an efficient means for RT decreasing. Hemovigilance system has to be improved so that all TR be reported. [less ▲]

Detailed reference viewed: 25 (1 ULg)
Full Text
Peer Reviewed
See detailComment intégrer l'automesure de la pression artérielle dans la mise au point de l'hypertendu
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (2001), 56(8), 552-556

Blood pressure measurement is the cornerstone of the diagnosis and follow up of arterial hypertension. Because of its large variability, the office blood pressure measurement can hardly be trusted. The ... [more ▼]

Blood pressure measurement is the cornerstone of the diagnosis and follow up of arterial hypertension. Because of its large variability, the office blood pressure measurement can hardly be trusted. The blood pressure self measurement allows more precisely to select the hypertensive patients (BP > 135/85 mmHg), to follow with more accuracy patients with important blood pressure variability and to check the efficacy of antihypertensive treatment. The self blood pressure measurement is at the early stage of its scientific period of development but requires the use of strictly validated devices which only measure the blood pressure at the brachial level. [less ▲]

Detailed reference viewed: 206 (0 ULg)
Peer Reviewed
See detailLes toxidermies iatrogenes, une rancon de la quete de la sante
Fumal, I.; Sriha, B.; Paquet, Philippe ULg et al

in Revue Médicale de Liège (2001), 56(8), 583-91

Muco-cutaneous drug reactions are side effects secondary to the intake of various medications. They are quite frequent and diverse in their clinical presentation, pathogeny, histology and prognosis. It is ... [more ▼]

Muco-cutaneous drug reactions are side effects secondary to the intake of various medications. They are quite frequent and diverse in their clinical presentation, pathogeny, histology and prognosis. It is important to diagnose and adequately monitor the disease in its early evolving phase. [less ▲]

Detailed reference viewed: 27 (7 ULg)
Peer Reviewed
See detailMutations Inactivating Mitochondrial Genes in Chlamydomonas Reinhardtii
Remacle, Claire ULg; Duby, Franceline ULg; Cardol, Pierre ULg et al

in Biochemical Society Transactions (2001), 29(Pt 4), 442-6

Chlamydomonas reinhardtii is now becoming a useful model for the study of mitochondrial genetics in a photosynthetic organism. The small (15.8 kb) mitochondrial genome C. reinhardtii has been sequenced ... [more ▼]

Chlamydomonas reinhardtii is now becoming a useful model for the study of mitochondrial genetics in a photosynthetic organism. The small (15.8 kb) mitochondrial genome C. reinhardtii has been sequenced completely and all the genes have been identified. Several mutants inactivated in mitochondrial genes encoding components of the respiratory complexes I, III and IV have been characterized at the molecular level. Assembly of complex I in several mutant strains and mapping of mitochondrial mutations by recombinational analysis are also described. [less ▲]

Detailed reference viewed: 23 (7 ULg)
Full Text
Peer Reviewed
See detailContribution of MT1-MMP and of Human Laminin-5 Gamma2 Chain Degradation to Mammary Epithelial Cell Migration
Gilles, Christine ULg; Polette, M.; Coraux, C. et al

in Journal of Cell Science (2001), 114(Pt 16), 2967-76

Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We ... [more ▼]

Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migration using an in vitro outgrowth migration assay. Stronger expression of MT1-MMP was observed at the mRNA and at the protein level in cells at the periphery of the outgrowth. As shown by videomicroscopy, these cells were involved in an orientated cell migration, in contrast to stationary cells distant from the periphery. Furthermore, MT1-MMP was mainly distributed in lamellipodia of migratory cells, as well as at their basal surface in contact with the substrate. Laminin-5 (Ln-5), a recently described substrate for MT1-MMP, was deposited preferentially in the matrix by migratory cells. Fragments of the gamma2 subunit of Ln-5 were also identified in migratory cultures of MCF10A cells, attesting to its proteolytic degradation. These fragments corresponded in size to those we observed after incubation of purified human Ln-5 with the recombinant catalytic domain of human MT1-MMP. We also show that anti-Ln5 blocking antibodies, MMP inhibitors (BB94 and TIMP-2) and MT1-MMP antisense oligonucleotides significantly decreased MCF10A cell migration. Taken together, these observations demonstrate that MT1-MMP is spatially and temporally regulated during MCF10A cell migration, and suggest that MT1-MMP-mediated pericellular proteolysis of Ln-5 gamma2 chain could contribute to this process. [less ▲]

Detailed reference viewed: 69 (4 ULg)
Full Text
See detailL'Intérêt Herpétologique des carrières du Condroz oriental (Province de Liège, Belgique)
Graitson, Eric ULg

in Natura Mosana (2001), 53(2), 110-118

Detailed reference viewed: 41 (6 ULg)
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Syndrome dyskinetique majeur induit par la ranitidine
Fouddah, A.; Canivet, Jean-Luc ULg; Damas, Pierre ULg

in Revue Médicale de Liège (2001), 56(8), 548-551

We report a case of severe dyskinetic syndrome, consisting of intense myoclonia movements, associated with choreiform activity involving the face and upper extremities. The abnormal movements occurred in ... [more ▼]

We report a case of severe dyskinetic syndrome, consisting of intense myoclonia movements, associated with choreiform activity involving the face and upper extremities. The abnormal movements occurred in a patient with confusion and visual hallucinations. This syndrome had an abrupt onset in a patient recovering from coronary artery bypass surgery complicated by an early post-operative cardiac arrest and acute renal failure. Dyskinesia appeared several days after intravenous administration of ranitidine for stress ulcer prophylaxis. Several etiologies were raised in this case among which were post-anoxic myoclonia and metabolic encephalopathy. Cessation of histamine receptor blocker therapy for 48 hours was associated with return of normal cognitive function and disappearance of abnormal movements. This confirmed the iatrogenic nature of the syndrome related to administration of ranitidine. [less ▲]

Detailed reference viewed: 49 (0 ULg)
Peer Reviewed
See detailCascades of Popping Bubbles Along Air/Foam Interfaces
Vandewalle, Nicolas ULg; Lentz, J. F.

in Physical Review. E : Statistical, Nonlinear, and Soft Matter Physics (2001), 64(2, Pt 1), 21507

We report image analysis of popping bubbles during the collapsing of two-dimensional (2D) and 3D aqueous foams. Although temporal and spatial correlations between successive popping bubbles within ... [more ▼]

We report image analysis of popping bubbles during the collapsing of two-dimensional (2D) and 3D aqueous foams. Although temporal and spatial correlations between successive popping bubbles within avalanches are emphasized, the breaking of a soap film at the air/foam interface seems to be independent of (i) the topology, (ii) the local curvature, and (iii) the size of the popping bubble. Possible mechanisms for cascades of pops are proposed and discussed. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailRelationship between bone mass density and tensile strength of the skin in women
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Vanderplaetsen, S. et al

in European Journal of Clinical Investigation (2001), 31(8), 731-735

BACKGROUND: Skin and bone may be affected similarly during the climacteric and during long-term corticotherapy. Little is known about the correlation between the respective alterations in bone mass ... [more ▼]

BACKGROUND: Skin and bone may be affected similarly during the climacteric and during long-term corticotherapy. Little is known about the correlation between the respective alterations in bone mass density (BMD) and tensile strength of the skin. MATERIALS AND METHODS: A total of 100 women aged 19-88 years, receiving, or not, hormone replenishment therapy or systemic corticosteroids, were enrolled in the study. Tensile strength of the inner forearm skin was measured using the suction method operated in both the steep and progressive-force application modes. BMD was measured by dual X-ray absorptiometry at the hip, femoral neck and lumbar spine. RESULTS: Being heterogeneous, the population of volunteers yielded a wide range of BMD and cutaneous tensile strength values. However, significant correlations were found between BMD and tensile skin parameters. In particular, a positive correlation was yielded between the biological elasticity of skin and the BMD of the hip and femoral neck. CONCLUSION: An overall correlation is shown between skin elasticity on a relatively sun-protected area and cortico-trabecular BMD. [less ▲]

Detailed reference viewed: 9 (3 ULg)
Full Text
Peer Reviewed
See detailNeurotransmitters as Early Signals for Central Nervous System Development
Nguyen, Laurent ULg; Rigo, Jean-Michel; Rocher, Véronique et al

in Cell & Tissue Research (2001), 305(2), 187-202

During brain ontogenesis, the temporal and spatial generation of the different types of neuronal and glial cells from precursors occurs as a sequence of successive progenitor stages whose proliferation ... [more ▼]

During brain ontogenesis, the temporal and spatial generation of the different types of neuronal and glial cells from precursors occurs as a sequence of successive progenitor stages whose proliferation, survival and cell-fate choice are controlled by environmental and cellular regulatory molecules. Neurotransmitters belong to the chemical microenvironment of neural cells, even at the earliest stages of brain development. It is now established that specific neurotransmitter receptors are present on progenitor cells of the developing central nervous system and could play, during neural development, a role that has remained unsuspected until recently. The present review focuses on the occurrence of neurotransmitters and their corresponding ligand-gated ion channel receptors in immature cells, including neural stem cells of specific embryonic and neonatal brain regions. We summarize in vitro and in vivo data arguing that neurotransmitters could regulate morphogenetic events such as proliferation, growth, migration, differentiation and survival of neural precursor cells. The understanding of neurotransmitter function during early neural maturation could lead to the development of pharmacological tools aimed at improving adult brain repair strategies. [less ▲]

Detailed reference viewed: 58 (3 ULg)
Peer Reviewed
See detailReduced Glutathione System: Role in Cancer Development, Prevention and Treatment (Review)
Locigno, R.; Castronovo, Vincenzo ULg

in International Journal of Oncology (2001), 19(2), 221-36

Reduced glutathione (GSH), a ubiquitous thiol-containing tripeptide, is unanimously recognized to play a central role in cell biology. It is highly implicated in the cellular defense against xenobiotics ... [more ▼]

Reduced glutathione (GSH), a ubiquitous thiol-containing tripeptide, is unanimously recognized to play a central role in cell biology. It is highly implicated in the cellular defense against xenobiotics and naturally occurring deleterious compounds such as free radicals and hydroperoxides. Consequently, GSH is an essential actor in several human diseases including cancer and cardio-vascular diseases. Its implication in oncogenesis has led to the development of new strategies to improve both prevention and treatment of cancer. The present review proposes an in depth analysis or our current knowledge of the relations between GSH and cancer. [less ▲]

Detailed reference viewed: 43 (1 ULg)
Full Text
Peer Reviewed
See detailA unified approach to transient stability contingency filtering, ranking and assessment
Ernst, Damien ULg; Ruiz-Vega, Daniel; Pavella, Mania ULg et al

in IEEE Transactions on Power Systems (2001), 16(3), 435-443

This paper proposes a unified approach to contingency filtering, ranking and assessment in power system transient stability studies. The approach consists of two-block techniques in which the first block ... [more ▼]

This paper proposes a unified approach to contingency filtering, ranking and assessment in power system transient stability studies. The approach consists of two-block techniques in which the first block selects from a list of contingencies the a priori “interesting” ones, that the second block ranks and assesses. This Filtering, Ranking and Assessment (FILTRA) approach relies on SIME (for SIngle Machine Equivalent). SIME is a hybrid direct-time-domain stability method which combines accuracy and flexibility skills of time-domain methods with sound additional possibilities of direct methods. The FILTRA approach is fully general: it may adapt itself to the specifics of any power system (modeling, protection characteristics, etc.), any contingency scenario and mode of (in)stability (first- or multi-swing, local or inter-area mode) and any application context (planning, operation planning and real-time operation). The approach is illustrated on two EHV power systems, having different structures, control and protective devices. The adequacy of its filtering and ranking capabilities is illustrated in terms of performance criteria such as reliability (ability to consistently capture all dangerous contingencies), effectiveness (ability to avoid false alarms) and computational efficiency (ability to comply with real-time requirements). Further, the assessment task is shown to provide the operator with sound information and effective means of control. [less ▲]

Detailed reference viewed: 71 (3 ULg)
Full Text
Peer Reviewed
See detailThe functional anatomy of inhibition processes investigated with the Hayling task
Collette, Fabienne ULg; Van der Linden, Martial ULg; Delfiore, Guy et al

in Neuroimage (2001), 14(2), 258-267

The cortical areas involved in inhibition processes were examined with positron emission tomography (PET). The tasks administered to subjects were an adaptation of the Hayling test. In the first condition ... [more ▼]

The cortical areas involved in inhibition processes were examined with positron emission tomography (PET). The tasks administered to subjects were an adaptation of the Hayling test. In the first condition (response initiation), subjects had to complete sentences with a word clearly suggested by the context, whereas in the second condition (response inhibition), subjects had to produce a word that made no sense in the context of the sentence. Results indicated that the response initiation processes were associated to increases of activity in the left inferior frontal gyrus (BA 45/47), whereas response inhibition processes led to increases in a network of left prefrontal areas, including the middle (BA 9 and BA 10) and inferior (BA 45) frontal areas. [less ▲]

Detailed reference viewed: 63 (4 ULg)
Full Text
Peer Reviewed
See detailUse of fluorescent polyethylene glycols (PEGs) to assess intestinal permeability in vitro
Loret, S; Pirson, L; Nollevaux, G et al

in Pflügers Archiv : European Journal of Physiology (2001), 442(5), 187

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailQuantitative Analysis of the Stabilization by Substrate of Staphylococcus Aureus Pc1 Beta-Lactamase
Lejeune, Annabelle ULg; Vanhove, Marc; Lamotte-Brasseur, Josette et al

in Chemistry & Biology (2001), 8(8), 831-42

BACKGROUND: The stabilization of enzymes in the presence of substrates has been recognized for a long time. Quantitative information regarding this phenomenon is, however, rather scarce since the enzyme ... [more ▼]

BACKGROUND: The stabilization of enzymes in the presence of substrates has been recognized for a long time. Quantitative information regarding this phenomenon is, however, rather scarce since the enzyme destroys the potential stabilizing agent during the course of the experiments. In this work, enzyme unfolding was followed by monitoring the progressive decrease of the rate of substrate utilization by the Staphylococcus aureus PC1 beta-lactamase, at temperatures above the melting point of the enzyme. RESULTS: Enzyme inactivation was directly followed by spectrophotometric measurements. In the presence of substrate concentrations above the K(m) values, significant stabilization was observed with all tested compounds. A combination of unfolding kinetic measurements and enzymatic studies, both under steady-state and non-steady-state regimes, allowed most of the parameters characteristic of the two concurrent phenomena (i.e. substrate hydrolysis and enzyme denaturation) to be evaluated. In addition, molecular modelling studies show a good correlation between the extent of stabilization, and the magnitude of the energies of interaction with the enzyme. CONCLUSIONS: Our analysis indicates that the enzyme is substantially stabilized towards heat-induced denaturation, independently of the relative proportions of non-covalent Henri-Michaelis complex (ES) and acyl-enzyme adduct (ES*). Thus, for those substrates with which the two catalytic intermediates are expected to be significantly populated, both species (ES and ES*) appear to be similarly stabilized. This analysis contributes a new quantitative approach to the problem. [less ▲]

Detailed reference viewed: 45 (5 ULg)
Full Text
Peer Reviewed
See detailReactive blending of functional PS and PMMA: interfacial behavior of in situ formed graft copolymers
Yin, Zhihui; Koulic, Christian; Pagnoulle, Christophe et al

in Macromolecules (2001), 34(15), 5132-5139

ω-Isocyanate PMMA, α-anhydride PMMA, and PS-co-PSNH2 have been prepared by atom transfer radical polymerization (ATRP) with controlled molecular weights (104 and 3.5 × 104) and low polydispersity (1.2 ... [more ▼]

ω-Isocyanate PMMA, α-anhydride PMMA, and PS-co-PSNH2 have been prepared by atom transfer radical polymerization (ATRP) with controlled molecular weights (104 and 3.5 × 104) and low polydispersity (1.2). They have been used as precursors of PS-g-PMMA copolymers and let to react in the melt (170 °C, for 10 min) under moderate shear rate. The well-controlled molecular characteristics of these precursors are a substantial advantage to study the effect of the kinetics of the interfacial reaction on the phase morphology. When the grafting reaction is fast (NH2/anhydride pair) and low molecular weight chains are used, the interfacial reaction is quasi-complete and a nanophase morphology is observed, whereas limited reaction and formation of microphases are observed in all the other cases. A high reaction yield requires not only that the functional groups are highly mutually reactive but also that the interface is anytime made available to the functional polymers for the reaction to progress. Then, a nanophase morphology may be observed, which is that of the copolymer formed by the interfacial reaction. A low reaction yield is dictated by either a slow interfacial reaction or a slow diffusion of the copolymer away from the interface. In the latter case, the phases formed by the unreacted precursors are stabilized by the copolymer which resides at the interface. [less ▲]

Detailed reference viewed: 40 (1 ULg)
Full Text
Peer Reviewed
See detailStructural Determinants of Cold Adaptation and Stability in a Large Protein
D'Amico, Salvino ULg; Gerday, Charles ULg; Feller, Georges ULg

in Journal of Biological Chemistry (2001), 276(28), 25791-6

The heat-labile alpha-amylase from an antarctic bacterium is the largest known protein that unfolds reversibly according to a two-state transition as shown by differential scanning calorimetry. Mutants of ... [more ▼]

The heat-labile alpha-amylase from an antarctic bacterium is the largest known protein that unfolds reversibly according to a two-state transition as shown by differential scanning calorimetry. Mutants of this enzyme were produced, carrying additional weak interactions found in thermostable alpha-amylases. It is shown that single amino acid side chain substitutions can significantly modify the melting point T(m), the calorimetric enthalpy Delta H(cal), the cooperativity and reversibility of unfolding, the thermal inactivation rate constant, and the kinetic parameters k(cat) and K(m). The correlation between thermal inactivation and unfolding reversibility displayed by the mutants also shows that stabilizing interactions increase the frequency of side reactions during refolding, leading to intramolecular mismatches or aggregations typical of large proteins. Although all mutations were located far from the active site, their overall trend is to decrease both k(cat) and K(m) by rigidifying the molecule and to protect mutants against thermal inactivation. The effects of these mutations indicate that the cold-adapted alpha-amylase has lost a large number of weak interactions during evolution to reach the required conformational plasticity for catalysis at low temperatures, thereby producing an enzyme close to the lowest stability allowing maintenance of the native conformation. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailConsidérations sur l'analyse statistique de données sensorielles
Claustriaux, Jean-Jacques ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(3), 155-158

This publication introduces some steps to build an adequate statistical data analysis for sensory studies.

Detailed reference viewed: 31 (3 ULg)
Full Text
Peer Reviewed
See detailModular Structure, Local Flexibility and Cold-Activity of a Novel Chitobiase from a Psychrophilic Antarctic Bacterium
Lonhienne, T.; Zoidakis, J.; Vorgias, C. E. et al

in Journal of Molecular Biology (2001), 310(2), 291-7

The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature ... [more ▼]

The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature chitobiase ArChb possesses four functionally independent domains: a catalytic domain stabilized by Ca(2+), a galactose-binding domain and an immunoglobulin-like domain followed by a cell-wall anchorage signal, typical of cell-surface proteins from Gram-positive bacteria. Binding of saccharides was analyzed by differential scanning calorimetry, allowing to distinguish unequivocally the catalytic domain from the galactose-binding domain and to study binding specificities. The results suggest that ArChb could play a role in bacterium attachment to natural hosts. Kinetic parameters of ArChb demonstrate perfect adaptation to catalysis at low temperatures, as shown by a low activation energy associated with unusually low K(m) and high k(cat) values. Thermodependence of these parameters indicates that discrete amino acid substitutions in the catalytic center have optimized the thermodynamic properties of weak interactions involved in substrate binding at low temperatures. Microcalorimetry also reveals that heat-lability, a general trait of psychrophilic enzymes, only affects the active site domain of ArChb. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Peer Reviewed
See detailMechanism of Colon Cancer Cell Apoptosis Mediated by Pyropheophorbide-a Methylester Photosensitization
Matroule, Jean-Yves; Carthy, Chris M; Granville, David J et al

in Oncogene (2001), 20

Pyropheophorbide-a methylester (PPME) is a second generation of photosensitizers used in photodynamic therapy (PDT). We demonstrated that PPME photosensitization triggered apoptosis of colon cancer cells ... [more ▼]

Pyropheophorbide-a methylester (PPME) is a second generation of photosensitizers used in photodynamic therapy (PDT). We demonstrated that PPME photosensitization triggered apoptosis of colon cancer cells as measured by using several classical parameters such as DNA laddering, PARP cleavage, caspase activation and mitochondrial release of cytochrome c. Preincubation of cells with N-acetyl cysteine (NAC) or pyrolidine dithiocarbamate (PDTC) protected against apoptosis mediated by PPME photosensitization showing that reactive oxygen species (ROS) are involved as second messengers. On the other hand, photosensitization carried out in the presence of deuterium oxide (D2O) which enhances singlet oxygen (1O2) lifetime only increases necrosis without affecting apoptosis. Since PPME was localized in the endoplasmic reticulum (ER)/Golgi system and lysosomes, other messengers than ROS were tested such as calcium, Bid, Bap31, phosphorylated Bcl-2 and caspase-12 but none was clearly identified as being involved in triggering cytochrome c release from mitochondria. On the other hand, we demonstrated that the transduction pathways leading to NF-kappaB activation and apoptosis were clearly independent although NF-kappaB was shown to counteract apoptosis mediated by PPME photosensitization. [less ▲]

Detailed reference viewed: 16 (0 ULg)