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See detailReliability of the TEC computed using GPS measurements: the problem of hardware biases
Warnant, René ULg

in Acta Geodaetica et Geophysica Hungarica (1997), 32(3-4), 451-459

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See detailVerapamil, a phenylalkylamine Ca2+ channel blocker, inhibits ATP-sensitive K+ channels in insulin-secreting cells
Lebrun, P.; Antoine, M.-H.; Ouedraogo, R. et al

in Diabetologia (1997), 40

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See detail"Saint Lambert au banquet de Jupille". Auguste Chauvin (1810-1884) et la peinture d'histoire
George, Philippe ULg

in Bulletin de la Société Royale Le Vieux-Liège (1997), T. XIII, n° 276

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See detailAutoregulation of endogenous glucose production in man.
Tappy, L.; Tounian, P.; Paquot, Nicolas ULg

in Biochemical Society Transactions (1997), 25(1), 11-3

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See detailRoquentin et les types sans importance sociale
Denis, Benoît ULg

in Etudes Françaises (1997), XXXIII(3), 105-119

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See detailCrystallization and preliminary X-ray analysis of bacteriophage lambda lysozyme in which all tryptophans have been replaced by aza-tryptophans
Evrard, Christine ULg; Declercq, Jean-Paul; Fastrez, Jacques

in Acta Crystallographica Section D-Biological Crystallography (1997), D53

After many unsuccessful attempts to crystallize the bacteriophage lambda lysozyme, a mutant where all the tryptophan residues have been replaced by aza-tryptophans has been crystallized by the vapor ... [more ▼]

After many unsuccessful attempts to crystallize the bacteriophage lambda lysozyme, a mutant where all the tryptophan residues have been replaced by aza-tryptophans has been crystallized by the vapor-diffusion method. The crystals are orthorhombic and belong to space group P212121 with cell dimensions a = 73.01, b = 78.80, c = 82.31 Å. Diffraction data were collected using synchrotron radiation sources. Crystals diffract to a resolution of 2.3 Å. Data from two different platinum derivatives were also recorded to 2.8 and 2.5 Å, respectively. [less ▲]

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See detailDesign for an Ipriflavone Multicenter European Fracture Study
Reginster, Jean-Yves ULg; Bufalino, L.; Christiansen, C. et al

in Calcified Tissue International (1997), 61(Suppl 1), 28-32

In order to investigate the efficacy of ipriflavone (i.p.) on the prevention of vertebral fractures and the effect on bone mineral density (BMD) in women with postmenopausal osteoporosis, a large ... [more ▼]

In order to investigate the efficacy of ipriflavone (i.p.) on the prevention of vertebral fractures and the effect on bone mineral density (BMD) in women with postmenopausal osteoporosis, a large multicentric European study was designed and is presently ongoing. Included in the study were 460 Caucasian, nonobese postmenopausal women aged > 45 and < 75 years, menopaused for at least 12 months. Inclusion was on the basis of a lumbar bone mineral density (BMD) lower than 2 SD compared with healthy women aged 50 years, corresponding to values below 0.860 g/cm2 (antero-posterior measurement) by Hologic QDR 1000. Women with prevalent vertebral fractures were excluded as well as those presenting secondary osteoporosis or having been treated with medications that could affect bone metabolism. This study was designed as a 3-year, double-blind, placebo-controlled, parallel group study that randomized the women to the oral administration of either 3 x 200 mg/day of i.p. or placebo. All patients received a daily supplement of 500 mg calcium. The primary purpose of the study was to evaluate the efficacy of i.p. in preventing vertebral nontraumatic fractures. Fracture is defined here as a > or = 20% decrease in any anterior, central, or posterior T4-L4 vertebral height. Blinded vertebral X-ray readings and vertebral morphometry have been centralized in an independent Center, with standardized evaluation of two experts. Power calculations have been based on the hypothesis that 21% of placebo-treated patients would fracture within 3 years and that treatment with i.p. would lead to a 50% reduction in the incidence of fracture. Statistical tests have been designed to have a power of 80%, with a type I error equal to 5%. Secondary endpoints were changes in vertebral, radial, and femoral BMD. Centralized controls on 100% BMD scans would ensure the good quality of BMD readings. This study should verify the hypothesis that i.p. significantly decreases the risk of vertebral fracture in postmenopausal, osteoporotic women. [less ▲]

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See detailAnaemia of lung cancer is due to impaired erythroid marrow response to erythropoietin stimulation as well as relative inadequacy of erythropoietin production.
Dowlati, A.; R'Zik, Samir ULg; Fillet, Georges ULg et al

in British Journal of Haematology (1997), 97(2), 297-9

Many studies have been done in order to elucidate the pathogenesis of the anaemia of chronic disorders accompanying cancer, with conflicting results. This is probably due to the heterogeneity of the ... [more ▼]

Many studies have been done in order to elucidate the pathogenesis of the anaemia of chronic disorders accompanying cancer, with conflicting results. This is probably due to the heterogeneity of the patient population selected for these studies (many patients treated by chemotherapy). To avoid this pitfall, in this study a very homogenous group of chemotherapy and radiotherapy-naive patients with lung cancer were selected. Serum erythropoietin and soluble transferrin receptor measurements suggested that the anaemia of non-treated lung cancer is mainly due to an impaired erythroid marrow response to erythropoietin stimulation. However, a relative inadequacy of erythropoietin production may also contribute. [less ▲]

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See detailFunctional anatomy of verbal and visuospatial span tasks in Alzheimer's disease
Collette, Fabienne ULg; Salmon, Eric ULg; Van der Linden, Martial ULg et al

in Human Brain Mapping (1997), 5(2), 110-118

The aim of the study was to emphasize cerebral regions which subserve the performance of short-term memory tasks in patients with Alzheimer's disease. We correlated scores obtained on span tasks with ... [more ▼]

The aim of the study was to emphasize cerebral regions which subserve the performance of short-term memory tasks in patients with Alzheimer's disease. We correlated scores obtained on span tasks with cerebral metabolism measured at rest with positron emission tomography. Scores obtained on the digit span task correlated with glucose metabolism in a brain area centered on the premotor cortex and extending to the adjacent motor and parietal gyri. There exists some evidence suggesting that this area may subserve the sequential organization of material stored in short-term memory. In a secondary analysis, we also observed significant interregional correlations between left-sided brain areas which are part of the neural network subserving verbal working memory processes in healthy controls. These data suggest that individual performance on verbal span tasks in AD patients may essentially depend on the preservation of their ordination processing capacity. The absence of correlation with prefrontal regions suggests that AD patients might not spontaneously engage central executive resources to reach their maximal span score. For simultaneous visuospatial span task, the performance of patients correlated with posterior brain regions, and not with prefrontal cortices. Hum. Brain Mapping 5:110-118, 1997. © 1997 Wiley-Liss Inc. [less ▲]

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See detailIron management in patients on rHuEpo
Cavill, I.; Macdougall, I. C.; Gokal, R. et al

in British Journal of Renal Medicine (1997), 2

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See detailPost-operative adjuvant therapy for non-small-cell lung cancer
Barthelemy, Nicole ULg; Johnson; Arriagada et al

in Lung Cancer (1997), 17(supp 1),

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See detailVrbis-Orbis (Ovide, Fastes, II, 684)
Rochette, Bruno ULg

in Latomus : Revue d'Etudes Latines (1997), 56(3), 551-553

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See detailPhénomènes karstiques dans les roches plutoniques et métamorphiques du sud Cameroun
Vicat, Jean-Paul; Lips, Bernard; Pouclet, André et al

in Karstologia (1997), 29

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See detailStepwise introduction of pi-electron cross-conjugaison: a possible access to [5] radialenes ?
Geneste, Florence; Moradpour, Alec; Dive, Georges ULg

in Journal of Organic Chemistry (1997), 62(16), 5339-5343

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See detailUne maison médiévale à Dinant
Hoffsummer, Patrick ULg

in Annales de la Société Archéologique de Namur (1997), 71

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See detailLow Thiamine Diphosphate Levels in Brains of Patients with Frontal Lobe Degeneration of the Non-Alzheimer's Type
Bettendorff, Lucien ULg; Mastrogiacomo, Frank; Wins, Pierre et al

in Journal of Neurochemistry (1997), 69(5), 2005-2010

We compared the thiamine and thiamine phosphate contents in the frontal, temporal, parietal, and occipital cortex of six patients with frontal lobe degeneration of the non-Alzheimer's type (FNAD) or ... [more ▼]

We compared the thiamine and thiamine phosphate contents in the frontal, temporal, parietal, and occipital cortex of six patients with frontal lobe degeneration of the non-Alzheimer's type (FNAD) or frontotemporal dementia with five age-, postmortem delay-, and agonal status-matched control subjects. Our results reveal a 40-50% decrease in thiamine diphosphate (TDP) in the cortex of FNAD patients, whereas thiamine monophosphate was increased 49-119%. TDP synthesizing and hydrolyzing enzymes were unaffected. The activity of citrate synthase, a mitochondrial marker enzyme, was decreased in the frontal cortex of patients with FNAD, but no correlation with TDP content was found. These results suggest that decreased contents of TDP, which is essentially mitochondrial, is a specific feature of FNAD. As TDP is an essential cofactor for oxidative metabolism and neurotransmitter synthesis, and because low thiamine status (compared with other species) is a constant feature in humans, a nearly 50% decrease in cortical TDP content may contribute significantly to the clinical symptoms observed in FNAD. This study also provides a basis for a trial of thiamine, to improve the cognitive status of the patients. [less ▲]

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See detailDopamine 'D2-like' receptor agonists in combination with cocaine: absence of interactive effects on locomotor activity.
Tirelli, Ezio ULg; Reggers, Jean ULg; Terry, P.

in Behavioural Pharmacology (1997), 8(2-3), 147-59

This study examined interactions between cocaine and drugs that act as direct agonists at subtypes of "D2-like" dopamine receptors. The drugs 7-OH-DPAT, quinpirole and RU24213 were studied alone and in ... [more ▼]

This study examined interactions between cocaine and drugs that act as direct agonists at subtypes of "D2-like" dopamine receptors. The drugs 7-OH-DPAT, quinpirole and RU24213 were studied alone and in combination with cocaine for their effects on locomotor activity in non-habituated mice. Locomotor activity was measured by photobeam crossings over 140 min. At the doses given (7-OH-DPAT: 0.006-6.4 mg/kg; quinpirole: 0.001-1 mg/kg; RU24213: 0.008-8 mg/kg) all three direct agonists dose-dependently reduced locomotor activity throughout the test, whereas cocaine (0.6-20 mg/kg) produced dose-related hyperactivity. Next, for each direct agonist, a series of doses was selected (up to threshold behaviourally-active doses) as pretreatments to a sub-maximally stimulant dose of cocaine (15 mg/kg). 7-OH-DPAT and quinpirole did not modulate the effects of cocaine; RU24213 produced, at best, a very modest attenuation of the effects of cocaine. Finally, a series of cocaine doses (below stimulant threshold) was given before a single dose of each direct agonist (the lowest dose to reduce activity significantly). Cocaine did not reliably alter the hypoactivity produced by any of the D2-like agonists. By demonstrating negligible interactions between cocaine and D2-like agonists, the results fail to demonstrate any necessary involvement of D2-like receptors in one of the behavioural effects of cocaine. [less ▲]

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See detailThe effect of dispersion and atomisation conditions on the compaction response of silicon nitride granules
Destoop, Vincent; Mertens, Anne ULg; Hendrix, W. et al

in Key Engineering Materials [=KEM] (1997), 132-136

The relationship between the dispersion conditions of silicon nitride slurries and the compaction behaviour of spray dried granules is reported in the case of two commercial powders. Two parameters have ... [more ▼]

The relationship between the dispersion conditions of silicon nitride slurries and the compaction behaviour of spray dried granules is reported in the case of two commercial powders. Two parameters have been retained: the solid loading, varied between 10 and 30 vol.% and the degree of stability of the suspension, controlled by adjustment of the pH. [less ▲]

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See detailCompte rendu de L. Couloubaritsis, J.-J. Wunenburger, La couleur, Bruxelles, 1993 (Recueil, 4)
Marganne, Marie-Hélène ULg

in Archives Internationales d'Histoire des Sciences (1997), 47

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See detailGluonic content of the scalar meson nonet in a scaled effective Lagrangian
Jaminon, Martine ULg; Van den Bossche, Bruno

in Nuclear Physics A (1997), 619

A scaled SU(3) Nambu-Jona-Lasinio Lagrangian is used to compute the mass of the nine scalar mesons in the vacuum and the mass of the glueball. This chiral model provides a gluonic content to the scalar ... [more ▼]

A scaled SU(3) Nambu-Jona-Lasinio Lagrangian is used to compute the mass of the nine scalar mesons in the vacuum and the mass of the glueball. This chiral model provides a gluonic content to the scalar mesons as well as a quark content to the glueball. It is shown that a suitable choice of the vacuum gluon condensate allows reproduction of the experimental masses of the scalar mesons except for K*0(1430). This choice corresponds to a weak coupling between the gluon and quark condensates, giving f0(1500) or fJ(1710) which is nearly a pure glueball. [less ▲]

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See detailFreeze-drying of lactic acid bacteria : Lactobacillus acidophilus
Desmons, S.; Zgoulli, S.; Destain, Jacqueline ULg et al

in Mededelingen - Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent (1997), 62/4b

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See detailThe faint young sun climatic paradox: Influence of the continental configuration and of the seasonal cycle on the climatic stability
Longdoz, Bernard; François, Louis ULg

in Global and Planetary Change (1997), 14(3-4), 97-112

A quasi-three-dimensional climate model is used to study the early state of the Earth when the solar luminosity was 70% of the present value, Usually, climatic simulations going back to this period lead ... [more ▼]

A quasi-three-dimensional climate model is used to study the early state of the Earth when the solar luminosity was 70% of the present value, Usually, climatic simulations going back to this period lead to a completely frozen planet contrasting with the geologic evidences of sedimentary rock formation and thus of the presence of liquid water at the surface of the continents during the Archean (4.6-2.5 billion years before present). Here, several model simulations are performed for solar luminosities varying between 0.7 and 1 times the present value. Using the present-day continental configuration and taking the seasonal cycle into account, a steady state is found in which glaciation is complete but snow covers only some oceanic coasts, leaving the continents essentially snow-free. As a result, the albedo of the continental area is strongly reduced compared to that of the frozen ocean, Some continental temperatures can almost reach the freezing point of water in summer (-1 degrees C in the center of Eurasia). This result can be explained by the behavior of the detailed hydrologic cycle included in the model. During the decrease of the solar luminosity, the jump to a completely frozen Earth occurs when the solar luminosity reaches 0.86 times its present value. The behavior of the climatic system is substantially different with a global ocean configuration. In the absence of land surfaces, the meridional heat transport, explicitly calculated, is less effective and the glaciation of a model latitude zone does not lead to the glaciation of its equatorward neighbor. The climate instability is relatively local and the jump to the completely frozen state is much more progressive than in the case of the modem continental configuration. The role of the seasonal cycle in the paleoclimatic simulation is also studied. Due to the non-linearity of the model, removing the seasonal cycle drives the system to an increase of the annual mean planetary albedo and to a decrease of the relative value (0.82) of the critical solar luminosity at which the jump to the completely frozen solution occurs. [less ▲]

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See detailL'article 185, alinéa 2, du Code civil et les opérations sur titres
Partsch, Philippe-Emmanuel ULg

in Revue Pratique des Sociétés (1997)

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See detailCiclosporine et maladies systémiques
Andre, Béatrice ULg; Ribbens, Clio ULg; Kaye, O. et al

in Médecine et Hygiène (1997), 55

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See detailEtude de la taille des nanoparticules après incorporation dans des sphéroïdes gastrorésistants
Duvivier, F; Grandfils, Christian ULg; Léonard, F et al

in Journal de Pharmacie de Belgique (1997), 52(3), 123

Nanoparticles can be used as an attractive carrier for the controlled delivery of drugs by the oral route. This nanoparticular delivery system can protect an active ingredient from enzymatic degradations ... [more ▼]

Nanoparticles can be used as an attractive carrier for the controlled delivery of drugs by the oral route. This nanoparticular delivery system can protect an active ingredient from enzymatic degradations and enhance its absorption from the intestinal mucosa. We studied the development of a gastroresistant dosage form for the oral administration of nanoparticles. Fast disintegrating pellets were used for the administration of nanoparticles and for their transit to the site of absorption. A gastroresistant film was applied onto the pellets to protect the nanoparticles and/ or the active substance from the acid gastric environment. The integrity of the nanoparticle size was assessed after two critical manufacturing steps: the extrusion/spheronisation process and the coating process. The determination of the nanoparticles size distribution was performed by photon correlation spectroscopy (PCS). The comparison of the PCS results between the original nanoparticles and the nanoparticles incorporated in the pellets shows a very low aggregation. Moreover the coating process was not a critical step since it was not responsible for an additional agglomeration of the nanoparticles. In conclusion, our manufacturing process allows the redispersion of the nanoparticles with a little change of the size distribution. In a next study we well try to quantify the release of the nanoparticles from the pellets. [less ▲]

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See detailHuman TEF-5 is preferentially expressed in placenta and binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer
Jacquemin, Patrick; Martial, Joseph ULg; Davidson, Irwin

in Journal of Biological Chemistry (1997), 272(20), 12928-37

We report the cloning of a cDNA encoding the human transcription factor hTEF-5, containing the TEA/ATTS DNA binding domain and related to the TEF family of transcription factors. hTEF-5 is expressed in ... [more ▼]

We report the cloning of a cDNA encoding the human transcription factor hTEF-5, containing the TEA/ATTS DNA binding domain and related to the TEF family of transcription factors. hTEF-5 is expressed in skeletal and cardiac muscle, but the strongest expression is observed in the placenta and in placenta-derived JEG-3 choriocarcinoma cells. In correlation with its placental expression, we show that hTEF-5 binds to several functional enhansons of the human chorionic somatomammotropin (hCS)-B gene enhancer. We define a novel functional element in this enhancer comprising tandemly repeated sites to which hTEF-5 binds cooperatively. In the corresponding region of the hCS-A enhancer, which is known to be inactive, this element is inactivated by a naturally occurring single base mutation that disrupts hTEF-5 binding. We further show that the binding of the previously described placental protein f/chorionic somatomammotropin enhancer factor-1 to TEF-binding sites is disrupted by monoclonal antibodies directed against the TEA domain and that this factor is a proteolytic degradation product of the TEF factors. These results strongly suggest that hTEF-5 regulates the activity of the hCS-B gene enhancer. [less ▲]

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See detailThe TEL gene products: nuclear phosphoproteins with DNA binding properties.
Poirel, H.; Oury, Cécile ULg; Carron, Clémence et al

in Oncogene (1997)

The human TEL gene is involved in several 12p13 chromosomal abnormalities present in various human hematological malignancies, the most frequent being the t(12;21)(p13;q22), specific for childhood acute ... [more ▼]

The human TEL gene is involved in several 12p13 chromosomal abnormalities present in various human hematological malignancies, the most frequent being the t(12;21)(p13;q22), specific for childhood acute lymphoblastic leukemia. The predicted product of TEL harbours an amino acid region similar to the ETS DNA binding domain. We now report the isolation of the murine TEL cDNA and the characterization of the human TEL proteins. Human and murine TEL proteins are particularly homologous within their aminoterminal regions and their ETS domains. TEL proteins are nuclear and display specific DNA binding activity toward classical ETS binding sites. In addition, we show that TEL mRNAs initiate translation at either of the two first inframe ATGs (codon 1 and 43) to encode 50 kDa and 57 kDa TEL proteins. In vivo, each of these primary translational products is modified by multiple phosphorylation events. [less ▲]

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See detailLa survie des savoirs médiévaux dans la littérature populaire. Le rôle des incunables
Opsomer, Carmélia ULg

in Bulletin de la Société des Bibliophiles liégeois (1997), 23

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See detailThe Effects of Hypertonic Saline in Healthy and Diseased Animals
Cambier, Carole ULg; Ratz, V.; Rollin, Frédéric ULg et al

in Veterinary Research Communications (1997), 21(5), 303-316

In this review, the pharmacological effects of administering hypertonic solutions to both healthy animals and during experimentally induced diseases are considered with a view to understanding the ... [more ▼]

In this review, the pharmacological effects of administering hypertonic solutions to both healthy animals and during experimentally induced diseases are considered with a view to understanding the mechanisms behind the possible clinical efficacy of such treatment. The review focuses successively on haemorrhagic shock, endotoxic shock and hypokalaemic metabolic alkalosis. How hypertonic saline solutions affect oxygen transport by haemoglobin is also considered. [less ▲]

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See detailAerosol therapy in the equine species
Duvivier, Dominique Hannia; Votion, Dominique ULg; Vandenput, Sandrina ULg et al

in Veterinary Journal (1997), 154(3), 189-202

Inhalation therapy plays an increasing role in the management of equine respiratory disorders. This alternative to systematic treatment permits a high concentration of medication to act locally while ... [more ▼]

Inhalation therapy plays an increasing role in the management of equine respiratory disorders. This alternative to systematic treatment permits a high concentration of medication to act locally while minimizing side effects and residues. In human medicine, literature in this field is prolific and continuously renewed, whereas in veterinary medicine, applications of aerosol therapy are less extensive. This review considers the principles of action of the different types of devices used for inhalation, i.e., nebulization, metered-dose inhalation and dry powder inhalation, describes the technical and practical requirements for their use in the equine species and considers the advantages and disadvantages of each inhalation device. The pharmacological agents currently administered to horses by inhalation are also discussed. Perspectives of aerosol therapy in the equine species, including aerosols already used in human medicine and their potential applications for horses are described [less ▲]

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See detailSur le bilinguisme dans les armées d'Hannibal
Rochette, Bruno ULg

in Etudes Classiques (Namur, Belgium) (1997), 65

Examen des divers témoignages antiques relatifs à la question linguistique au sein de l'armée d'Hannibal. Fort hétéroclite par son recrutement, cette armée présentait inévitablement une grande bigarrure ... [more ▼]

Examen des divers témoignages antiques relatifs à la question linguistique au sein de l'armée d'Hannibal. Fort hétéroclite par son recrutement, cette armée présentait inévitablement une grande bigarrure linguistique. On y trouvait cependant des interprètes et des soldats capables de s'exprimer dans la langue des ennemis ou de s'improviser eux-mêmes interprètes. De fait il existait un bilinguisme associant la connaissance d'une ou de plusieurs langues locales, d'une part, et du grec ou du latin, d'autre part. [less ▲]

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See detailUse of anti inflammatory drugs in the treatment of bovine respiratory disease complex
Lekeux, Pierre ULg; VandeWeerdt, M. L.

in Israel Journal of Veterinary Medicine (1997), 52(2-3), 113-116

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See detailHypochlorous acid activates NF-kappaB transcription factor in T lymphocytes
Schoonbroodt, Sonia; Legrand-Poels, Sylvie ULg; Best-Belpomme, Martin et al

in Biochemical Journal (1997)

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See detailDesigning and implementing a GIS in an international context
Pantazis, Dimos N; Cornélis, Bernard ULg

in Transactions in GIS (1997), 1(4), 301-320

International collaborations between private companies, national research laboratories, and universities in the field of GIS have opened up new ways of exchanging knowledge and experiences between ... [more ▼]

International collaborations between private companies, national research laboratories, and universities in the field of GIS have opened up new ways of exchanging knowledge and experiences between different countries. However, when a collaboration involves the actual design and implementation of an operational GIS, rather than simply theoretical or applied research, or the development of specific applications, many problems can arise, sometimes leading either to the partial success or the complete failure of the project. Some of these problems are frequently encountered in all kinds of international collaborations: problems arising from different cultural mentalities, difficulties establishing a single common leadership, difficulties with communication and information exchange, and so on. Problems relating to the specific nature of the GIS are also present, for example the absence of digital geographic data format standards, the absence of metadata standards, the omission to use GIS or IS design methods, etc. This paper focuses on a joint Sino-Belgian project for the design and implementation of an urban geographical information system (UGIS) integrating remote sensing techniques in Hangzhou city, China and examines the general, specific, and international aspects of the problems encountered in an international collaboration. [less ▲]

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See detailIncorporation of rapeseed meal extracted by pressure in a diet for growing fattening bulls .2. Glucosinolates and their breakdown products in the rumen
Wathelet, Jean-Paul ULg; Istasse, Louis ULg; Mayombo, A. P. et al

in Annales de Zootechnie (1997), 46(1), 71-79

Eight bulls from the Belgian Blue breed were offered diets in which soya bean meal and linseed meal were substituted by rapeseed meal (variety Samourai) in order to provide, respectively, 33, 66 and 100 ... [more ▼]

Eight bulls from the Belgian Blue breed were offered diets in which soya bean meal and linseed meal were substituted by rapeseed meal (variety Samourai) in order to provide, respectively, 33, 66 and 100% of the protein. The glucosinolate content was equal to 17.6 mu mol/g dry matter. Analyses of wet residues from rapeseed meal incubated in the rumen showed a rapid decrease of the glucosinolate content which disappeared after 5 h of incubation. The 3-hydroxypent-4-enenitrile, the but-3-enylisothiocyanate and the 5-vinyl-1,3-oxazolidine-2-thione antinutritional factors obtained from the glucosinolates were identified by GC-MS during the first 5 h. The 3-phenylpropanenitrile, the 3-methylindol, the 2-phenylacetic and 3-phenylpropanoic acids were also present in the residues and in the rumen. Although high sensitivity of the methods were used, no glucosinolates and their direct breakdown products were detected in the liquid and the solid content of the rumen. [less ▲]

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See detailBiomarqueurs et bioindicateurs chez les vertébrés : importance pour l’évaluation de la santé d’un écosystème
Lessire, Françoise ULg; Delaunois, Annie; Gustin, Pascal ULg et al

in Annales de Médecine Vétérinaire (1997), 141

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See detailElectron tomography of metaphase nucleolar organizer regions: evidence for a twisted-loop organization.
Heliot, L.; Kaplan, H.; Lucas, L. et al

in Molecular Biology of the Cell (1997), 8(11), 2199-216

Metaphase nucleolar organizer regions (NORs), one of four types of chromosome bands, are located on human acrocentric chromosomes. They contain r-chromatin, i.e., ribosomal genes complexed with proteins ... [more ▼]

Metaphase nucleolar organizer regions (NORs), one of four types of chromosome bands, are located on human acrocentric chromosomes. They contain r-chromatin, i.e., ribosomal genes complexed with proteins such as upstream binding factor and RNA polymerase I, which are argyrophilic NOR proteins. Immunocytochemical and cytochemical labelings of these proteins were used to reveal r-chromatin in situ and to investigate its spatial organization within NORs by confocal microscopy and by electron tomography. For each labeling, confocal microscopy revealed small and large double-spotted NORs and crescent-shaped NORs. Their internal three-dimensional (3D) organization was studied by using electron tomography on specifically silver-stained NORs. The 3D reconstructions allow us to conclude that the argyrophilic NOR proteins are grouped as a fiber of 60-80 nm in diameter that constitutes either one part of a turn or two or three turns of a helix within small and large double-spotted NORs, respectively. Within crescent-shaped NORs, virtual slices reveal that the fiber constitutes several longitudinally twisted loops, grouped as two helical 250- to 300-nm coils, each centered on a nonargyrophilic axis of condensed chromatin. We propose a model of the 3D organization of r-chromatin within elongated NORs, in which loops are twisted and bent to constitute one basic chromatid coil. [less ▲]

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See detailApplication of canonical transformation with missing values to multitrait evaluation of Jersey type
Gengler, Nicolas ULg; Wiggans, G. R.; Wright, J. R. et al

in Journal of Dairy Science (1997), 80(10), 2563-2571

A multitrait animal model was used to calculate predicted transmitting ability and reliabilities for final score and 15 linear type traits of 225,632 US Jersey cows. Records were adjusted for age and ... [more ▼]

A multitrait animal model was used to calculate predicted transmitting ability and reliabilities for final score and 15 linear type traits of 225,632 US Jersey cows. Records were adjusted for age and stage of lactation before analysis. The model contained effects for interactions of herd and date scored; year scored, parity, and age; and herd and sire; effects of permanent environment and additive genetics were also included. Of the 381,511 records included, some observations were missing for final score (8%), body depth (43%), and teat length (33%). The evaluation system used a canonical transformation, included several random effects, and estimated missing values with each iteration. Inbreeding was considered in the computations. Convergence was achieved in approximately 50 rounds of iteration. Correlations between animal and sire model predicted transmitting ability ranged from 0.56 to 0.95 and generally were higher for bulls than for cows and for more recent birth years. Genetic trend was strongly positive for dairy form, final score, and rear udder traits (height and width) and negative for udder depth. For other traits, genetic trend was small. This methodology should improve the accuracy of genetic evaluations for type traits of US Jerseys. [less ▲]

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See detailPredictions in overdispersed series of counts using an approximate predictive likelihood
Lambert, Philippe ULg

in Journal of Forecasting (1997), 16

The generalized autoregression model or GARM, originally used to model series of non-negative data measured at irregularly spaced time points (Lambert, 1996a), is considered in a count data context. It is ... [more ▼]

The generalized autoregression model or GARM, originally used to model series of non-negative data measured at irregularly spaced time points (Lambert, 1996a), is considered in a count data context. It is first shown how the GARM can be expressed as a GLM in the special case of a linear model for some transform of the location parameter. The Butler approximate predictive likelihood (Butler, 1986, Rejoinder) is then used to define likelihood prediction envelopes. The width of these intervals is shown to be slightly wider than the Fisher (1959, pp. 128-33) and Lejeune and Faulkenberry (1982) predictive likelihood-based envelopes which assume that the parameters have fixed known values (equal to their maximum likelihood estimates). The method is illustrated on a small count data set showing overdispersion. [less ▲]

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See detailEvidence for a Non-Gabaergic Action of Quaternary Salts of Bicuculline on Dopaminergic Neurones
Seutin, Vincent ULg; Scuvée-Moreau, Jacqueline ULg; Dresse, Albert ULg

in Neuropharmacology (1997), 36(11-12, Nov-Dec), 1653-7

Intracellular recordings were made from neurones, presumed to be dopaminergic, in the rat midbrain slice preparation. Bicuculline methiodide (BMI) and methochloride (BMC) reversibly blocked the slow ... [more ▼]

Intracellular recordings were made from neurones, presumed to be dopaminergic, in the rat midbrain slice preparation. Bicuculline methiodide (BMI) and methochloride (BMC) reversibly blocked the slow, apamin-sensitive component of the afterhyperpolarization in these cells. The IC50 for this effect was about 26 microM. In comparison, BMC antagonized the input resistance decrease evoked by muscimol (3 microM) with an IC50 of 7 microM. The base of bicuculline was less potent in blocking the slow afterhyperpolarization. SR95531 (2-[carboxy-3'-propyl]-3-amino-6-paramethoxy-phenyl-pyridaziniu m bromide), another competitive GABA(A) antagonist, and picrotoxin, a non-competitive GABA(A) antagonist, also blocked the action of muscimol (IC50s: 2 and 12 microM respectively), but had no effect on the afterhyperpolarization at a concentration of up to 100 microM. Moreover, 100 microM SR95531 did not affect the blockade of the afterhyperpolarization by BMC. This blockade persisted in the presence of tetrodotoxin and was apparently not due to a reduction of calcium entry, suggesting that it involved a direct action on the channels which mediate this afterhyperpolarization. These results strongly suggest that quaternary salts of bicuculline act on more than one target in the central nervous system. Thus, the involvement of GABA(A) receptors in a given effect cannot be proven solely on the basis of its blockade by these agents. [less ▲]

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See detailSuivi satellitaire de la migration automnale de la cigogne noire. Aléas et perspectives d'avenir
Libois, Roland ULg; Jadoul, Gérard

in Aves (1997), 34(1), 20-22

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See detailA novel seizure-induced synaptotagmin gene identified by differential display.
Babity, J. M.; Armstrong, J. N.; Plumier, Jean-Christophe ULg et al

in Proceedings of the National Academy of Sciences of the United States of America (1997), 94(6), 2638-41

Systemic administration of kainic acid, a cyclic analogue of glutamate, produces many of the clinical features of human temporal lobe epilepsy and status epilepticus in rats, including the induction of ... [more ▼]

Systemic administration of kainic acid, a cyclic analogue of glutamate, produces many of the clinical features of human temporal lobe epilepsy and status epilepticus in rats, including the induction of motor convulsions and the degeneration of neurons in the hippocampus and piriform cortex. Differential display PCR was used to identify mRNAs that are differentially expressed between degenerating and nondegenerating tissues in the brain after kainic acid-induced seizure activity. A novel cDNA fragment expressed in the degenerating hippocampus and piriform cortex, but not in the nondegenerating parietal cortex, was identified, cloned, and sequenced. This novel cDNA fragment identified a new member of the synaptotagmin gene family that is rapidly and transiently induced in response to seizure activity. Differential expression of this synaptotagmin gene, syt X, was confirmed by Northern blot analysis and in situ hybridization. This novel, inducible synaptotagmin gene may provide a direct link between seizure-induced neuronal gene expression and subsequent modulation of synaptic structure and function. [less ▲]

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See detailThyroid hormone inhibits the human prolactin gene promoter by interfering with activating protein-1 and estrogen stimulations
Pernasetti, Flavia; Caccavelli, L.; Van de Weerdt, Cécile ULg et al

in Molecular Endocrinology (Baltimore, Md.) (1997), 11(7), 986-96

Transcription of the human PRL (hPRL) gene in the pituitary is subject to tissue-specific and multihormonal regulation involving two main regulatory regions, a proximal promoter and a distal enhancer. In ... [more ▼]

Transcription of the human PRL (hPRL) gene in the pituitary is subject to tissue-specific and multihormonal regulation involving two main regulatory regions, a proximal promoter and a distal enhancer. In this report we show that thyroid hormone inhibits the expression of the hPRL gene in rat pituitary cells. Transient expression experiments show that thyroid hormone regulation involves a strong inhibitory element, located in the proximal (-164/-35) promoter, which is modulated by a more distal stimulatory response control region. Gel retardation experiments reveal that the thyroid hormone receptor does not bind to the proximal negative element. We show the existence of an activating protein-1 (AP-1) response element located at positions -61 to -54 of the proximal promoter, conferring AP-1 stimulation to the hPRL promoter. This AP-1 induction is abolished when hormone-bound thyroid hormone receptor is present, indicating that there is an interference between the thyroid hormone receptor and AP-1 regulatory pathways. Furthermore, using the complete hPRL upstream region, we show that estrogen induction is abolished by simultaneous thyroid hormone treatment. [less ▲]

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See detailThe Nucleotide Sequence Of Saccharomyces Cerevisiae Chromosome XII
Johnston, M.; Hillier, L.; Riles, L. et al

in Nature (1997), 387(6632),

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See detailRuptured Aneurysm of the Profunda Femoral Artery Associated with Polyaneurysmal Disease
Defraigne, Jean-Olivier ULg; Vasquez, C.; Limet, Raymond ULg

in Acta Chirurgica Belgica (1997), 97(2), 93-6

A 73-year-old man was admitted for rupture of an atherosclerotic aneurysm of the profunda femoris artery associated to popliteal and bilateral axillary arteries aneurysms. The aneurysm of the profunda ... [more ▼]

A 73-year-old man was admitted for rupture of an atherosclerotic aneurysm of the profunda femoris artery associated to popliteal and bilateral axillary arteries aneurysms. The aneurysm of the profunda femoris artery was developed between the branches of the crural nerves. Considering the patency of the superficial femoral artery and of the leg arterie, and to avoid injury to the crural nerve, no reconstruction of the profunda femoris was attempted. The aneurysm was ligated proximally and buttress sutures were placed on the site of rupture to achieve thrombosis of the aneurysms. Most of the published cases of aneurysm of the profunda femoris artery are false aneurysms developed after various trauma. Atherosclerotic aneurysm of the profunda femoris artery is a rare lesion and rupture is exceptional. [less ▲]

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See detailEstimation of (co)variance components for Jersey type traits using a repeatability model
Gengler, Nicolas ULg; Wiggans, G. R.; Wright, J. R. et al

in Journal of Dairy Science (1997), 80(8), 1801-1806

(Co)variance components for final score and 15 linear type traits of Jersey cows were estimated by multitrait REML using multiple diagonalization and a repeatability model with 34,999 records of 22,354 ... [more ▼]

(Co)variance components for final score and 15 linear type traits of Jersey cows were estimated by multitrait REML using multiple diagonalization and a repeatability model with 34,999 records of 22,354 cows. Multiple diagonalization gave relative off-diagonals (ratio of squared off-diagonals to the product of diagonals) of <0.1%. Heritabilities and repeatabilities, respectively, were estimated as 0.29 and 0.48 for final score, 0.40 and 0.57 for stature, 0.26 and 0.39 for strength, 0.28 and 0.43 for dairy form, 0.13 and 0.25 for foot angle, 0.13 and 0.25 for rear legs (side view), 0.27 and 0.41 for body depth, 0.31 and 0.52 for rump angle, 0.22 and 0.33 for thurl width, 0.22 and 0.36 for fore udder attachment, 0.28 and 0.46 for rear udder height, 0.26 and 0.42 for rear udder width, 0.32 and 0.48 for udder depth, 0.20 and 0.36 for udder cleft, 0.29 and 0.46 for front teat placement, and 0.31 and 0.48 for teat length. Estimates of heritability generally were higher, and estimates of repeatability were lower, than values used previously for USDA genetic evaluations, which were based on data from the 1970s and early 1980s. Final score was highly correlated both genetically and phenotypically with dairy form and rear udder traits. These estimates of heritabilities and (co)variance components are necessary for multitrait genetic evaluation of linear type traits of US Jerseys. [less ▲]

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See detailRéflexions sur l’iconologie de la châsse de saint Remacle
Van den Bossche, Benoît ULg

in Bulletin de la Société d’Art et d’Histoire du Diocèse de Liège (1997), LXII

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See detailA 12 Kb Nucleotide Sequence Containing The Alanine Dehydrogenase Gene At 279 Degrees On The Bacillus Subtilis Chromosome
Oudega, B.; Vandenbol, Micheline ULg; Koningstein, G.

in Microbiology-Uk (1997), 143

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See detailThe role of bisphosphonates in the treatment of osteoporosis
Reginster, Jean-Yves ULg; Halkin, V.; Gosset, Christiane ULg et al

in Drugs of Today (Barcelona, Spain : 1998) (1997), 33

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See detailPrevalence and characterization of Microsporum canis carriage in cats.
Mignon, Bernard ULg; Losson, Bertrand ULg

in Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology (1997), 35(4), 249-256

In order to determine the prevalence and to characterize the carriage of Microsporum canis in cats, different mycological examinations (including a culture obtained by hair brushing and Wood's light ... [more ▼]

In order to determine the prevalence and to characterize the carriage of Microsporum canis in cats, different mycological examinations (including a culture obtained by hair brushing and Wood's light examination) were performed on 632 animals of different origins. Group 1 comprised 467 healthy pet cats belonging to veterinary students. In this group, prevalence of carriage was 2.1%: eight cats were asymptomatic transient carriers and one cat was an asymptomatic infected animal presenting discrete Wood's-positive lesions disseminated on the whole body that were visible after sedation and clipping. The carriage prevalence was higher (15.7%) in group 2 comprising 134 European cats destroyed in a pound and kept together. In two additional groups of cats, it was shown that an infected cat was responsible for the dissemination of fungal material into its environment including the other in-contact animals. When the active source of fungus was removed, the dissemination stopped, resulting in a decrease in the amount of infective material recovered from both the animal carriers and the environmental surfaces. This was also observed in two experimental groups of guinea pigs. No association between feline immunodeficiency virus infection and the M. canis carriage was observed in a retrospective case-control study performed on group 2. None of these cats was feline leukaemia virus positive. [less ▲]

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See detailDilatometric and calorimetric study of the graphitisation of high chromium cast iron
Terziev, L.; Lecomte-Beckers, Jacqueline ULg; Bojinov, V. et al

in Journal de Chimie Physique et de Physico-Chimie Biologique (1997), 94[5]

Cette étude est consacrée à la formation du graphite dans des fontes à haute teneur en chrome (8%) et spécialement à la détermination des conditions de formation de graphite secondaire par des traitements ... [more ▼]

Cette étude est consacrée à la formation du graphite dans des fontes à haute teneur en chrome (8%) et spécialement à la détermination des conditions de formation de graphite secondaire par des traitements thermiques adaptés. La solidification de la fonte étudiée a été examinée par analyse thermique différentielle (ATD). Les effets d'un traitement à haute température ont été examinés à la fois par calorimétrie et dilatomètre différentielle. Le graphite primaire se forme vers 950°C. Le graphite secondaire précipite sur le graphite primaire lors du traitement de déstabilisation de l'austénite à haute température. La forme et la quantité de graphite secondaire dépendent de la température de traitement. [less ▲]

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See detailCompte rendu de A. ANGENENDT, Heilige und Reliquien, Munich, 1994
George, Philippe ULg

in Francia. 1, Mittelalter (1997)

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See detailOrigine sociale et réussite scolaire : un modèle en Chaîne de Markov estimé sur données individuelles belges
Donni, Olivier; Lejeune, Bernard ULg

in Recherches Economiques de Louvain (1997), 63(4), 421-446

Cet article étudie les relations entre l'origine socio-économique des élèves belges et leur parcours scolaire primaire et secondaire. Il repose sur l'hypothèse que le parcours scolaire des élèves peut ... [more ▼]

Cet article étudie les relations entre l'origine socio-économique des élèves belges et leur parcours scolaire primaire et secondaire. Il repose sur l'hypothèse que le parcours scolaire des élèves peut être décrit par un processus de Markov. Sur la base d'un échantillon transversal d'un millier d'enfants, en fonction de variables socio-économiques et pour chacune des années de scolarité obligatoire, les probabilités individuelles de poursuivre un parcours scolaire ''normal'', c.-à-d. sans redoublement ni relégation dans une filière de second choix, sont estimées. Nos résultats montrent que derrière les substantiels taux agrégés de redoublement qui caractérisent le système scolaire belge se cachent en fait de profondes inégalités sociales. [less ▲]

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See detailCalibration of a finite element model for bolted endplate steel connections
Bursi, O.; Jaspart, Jean-Pierre ULg

in Journal of Constructional Steel Research (1997), 44(No 3), 225-262

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See detailAge model estimation in paleoclimatic research : fuzzy regression and radiocarbon uncertainties
Boreux, Jean-Jacques ULg; Pesti, G.; Duckstein, L. et al

in Palaeogeography, Palaeoclimatology, Palaeoecology (1997), 128(1), 29-37

A fuzzy linear regression-based method to relate the depth and age of sediment layers is described. Because algae, moss or local plants respond to climatic change, the age and depth of sediment layers are ... [more ▼]

A fuzzy linear regression-based method to relate the depth and age of sediment layers is described. Because algae, moss or local plants respond to climatic change, the age and depth of sediment layers are interrelated variables but the link between them is often imprecise. In most cases, due to the limited number of layers in a core (i.e., small number of data points), estimation of the slope and uncertainties in classical regression analysis does not take into account the uncertainties in radiocarbon dating. Here, fuzzy linear regression, which may be applied even to a very small data set, is utilized. The method, illustrated through a practical example, with eight data points, appears to be a promising tool in stratigraphic studies to link sediment age to layer depth and takes into account uncertainties from radiocarbon dating. [less ▲]

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See detailAttitude des élèves face à l'école et au cours d'éducation physique, âge et retard scolaire
Piéron, Maurice; Delfosse, Catherine; Ledent, Maryse et al

in Revue de l'Education Physique (1997), 37(1), 31-41

Le terme motivation fait partie du langage habituel des psychologues, des enseignants, des sportifs,... Nous pourrions continuer cette liste sans parvenir à lui donner un caractère exhaustif. Parfois ... [more ▼]

Le terme motivation fait partie du langage habituel des psychologues, des enseignants, des sportifs,... Nous pourrions continuer cette liste sans parvenir à lui donner un caractère exhaustif. Parfois utilisé de manière abusive, ce concept présente néanmoins des aspects théoriques et pratiques fondamentaux. A la notion de motivation, on associe très fréquemment celle d'attitude. Toutes deux sont définies en référence à l'affect, c'est-à-dire à une impression d'attraction ou de répulsion vis-à-vis d'un objet déterminé. ... [less ▲]

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See detailAutomatic external filling for the ion source gas bottle of a Van de Graaff accelerator
Bastin, Thierry ULg; Dehove, C.; Dumont, Paul-Dominique ULg et al

in Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (1997), 129

We describe a fully automatic system we developed to fill, from an external gas bottle, the ion sourer terminal gas storage bottle of a 2 MV Van de Graaff accelerator without depressing the 25 bar ... [more ▼]

We describe a fully automatic system we developed to fill, from an external gas bottle, the ion sourer terminal gas storage bottle of a 2 MV Van de Graaff accelerator without depressing the 25 bar insulating gas. The system is based on a programmable automate ordering electropneumatical valves. The only manual operation is the connection of the external gas cylinder. The time needed for a gas change is reduced to typically 15 min (depending on the residual pressure wished for the gas removed from the terminal bottle). To check this system we study the ionic composition of the ion beam delivered by our accelerator after different gas changes. The switching magnet of our accelerator was used to analyse the ionic composition of the accelerated beams in order to verify the degree of elimination of the previous gases in the system. (C) 1997 Elsevier Science B.V. [less ▲]

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See detailPit-1 gene HinfI RFLP and growth traits in double-muscled Belgian Blue cattle
Renaville, Robert ULg; Gengler, Nicolas ULg; Parmentier, Isabelle et al

in Journal of Animal Science (1997), 75(1), 146

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See detailFamilial Crohn's Disease: A Study of 18 Families
Franchimont, D.; Belaiche, Jacques ULg; Louis, Edouard ULg et al

in Acta Gastro-Enterologica Belgica (1997), 60(2, Apr-Jun), 134-7

The high frequency of familial Crohn's disease (CD) suggests a genetic predisposition. The most recent data from epidemiology and molecular biology are consistent with a multifactorial, polygenic ... [more ▼]

The high frequency of familial Crohn's disease (CD) suggests a genetic predisposition. The most recent data from epidemiology and molecular biology are consistent with a multifactorial, polygenic inheritance with a possible genetic heterogeneity. The aim of our study was, first to compare familial and sporadic CD on the basis of the type and location of the disease, and age at diagnosis, and second, to evaluate among families, the concordance rate for the type and location of the disease. PATIENTS AND METHODS: 18 families with 2 (n = 16) and 3 (n = 2) affected first degree relatives were studied. They were compared to a population of 154 sporadic CD coming from the same gastroenterology unit. RESULTS: Age at diagnosis was the same in sporadic and familial CD. There was an increased frequency of ileal (p = 0.02), and fibro stenotic (p = 0.005) CD and a decreased frequency of colonic (p = 0.006) and inflammatory (p = 0.02) disease, in familial CD. There was a significant increase in concordance rate for fibrostenotic disease (p < 0.001) and a decrease for inflammatory disease (p < 0.01), among the families. The observed concordance rate for the location of the disease was not significantly different from the expected one. In conclusion, these data suggest that CD may be heterogenous and that different clinical patterns may be determined either by genetic or environmental factors. [less ▲]

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See detailStudy on the Production of Xylanolytic complex from Pencillium canescens 10-10c.
Gaspar, A.; Cosson, T.; Roques, C. et al

in Applied Biochemistry and Biotechnology (1997), 67

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See detailFirst epidemiological data on pathogenic leptospires isolated on the Azorean islands
Collares-Pereira, Margarida; Korver, H.; Terpstra, W. J. et al

in European Journal of Epidemiology (1997), 13

Insectivores (Erinaceus europaeus) and rodents (Rattus rattus, R. norvegicus and Mus musculus) from different islands of the Azores archipelago were found to carry three distinct Leptospira interrogans s ... [more ▼]

Insectivores (Erinaceus europaeus) and rodents (Rattus rattus, R. norvegicus and Mus musculus) from different islands of the Azores archipelago were found to carry three distinct Leptospira interrogans s.l. serovars (copenhageni, icterohaemorrhagiae and ballum) which have never been previously investigated there. The house mouse and the black rat were the major Leptospira reservoir showing isolation rates ranging from 0% for both species (in Graciosa) to 88 % and 33 %, respectively (in São Miguel). This study also showed that the majority of the animals with positive kidney cultures exhibited specific agglutinins agaibst the isolated strains of Leptospira. The observed isalation rates in the different islands, with a very interesting island variation in prevalence, suggest that small mammals, serving as sylvatic reservoirs of pathogenic leptospires, may represent an important risk to the health of humans and the livestock, particularly in the islands of Terceira and São Miguel. [less ▲]

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See detailOzone-Induced Stimulation of Pulmonary Sympathetic Fibers: A Protective Mechanism against Edema
Delaunois, Annie ULg; Segura, P.; Dessy, Cécile ULg et al

in Toxicology and Applied Pharmacology (1997), 147(1), 71-82

Tropospheric ozone exerts well-described toxic effects on the respiratory tract. Less documented, by contrast, is the ability of ozone to induce protective mechanisms against agents that are toxic to the ... [more ▼]

Tropospheric ozone exerts well-described toxic effects on the respiratory tract. Less documented, by contrast, is the ability of ozone to induce protective mechanisms against agents that are toxic to the lungs. In particular, interactions between ozone and the sympathetic nervous system have never been considered. Using a model of permeability edema in isolated perfused rabbit lungs, we report here that, immediately after exposure of rabbits to 0.4 ppm ozone for 4 hr, the pulmonary microvascular responses to acetylcholine and substance P are completely blocked. Several lines of evidence, including partial inhibition of the ozone-induced protective effect by several drugs (alpha2- and beta-adrenergic antagonists, neuropeptide Y antagonist, guanethidine), measured levels of released catecholamines in blood and urine and the in vitro response of isolated lungs exposed to 0.4 ppm ozone all seem to suggest that ozone can stimulate pulmonary adrenergic fibers and induce the local release of catecholamines and neuropeptide Y, this resulting in transient protection against pulmonary edema. We also showed that, 48 hr after the exposure, ozone increased the baseline microvascular permeability and the response to low concentrations of acetylcholine [less ▲]

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See detailCortical application of potassium chloride induces the low-molecular weight heat shock protein (Hsp27) in astrocytes.
Plumier, Jean-Christophe ULg; David, Jean-Claude; Robertson, H. A. et al

in Journal of Cerebral Blood Flow & Metabolism (1997), 17(7), 781-90

Spreading depression induces tolerance to ischemic injury, and ischemic tolerance has been associated with expression of heat shock proteins (Hsp). Here we examine Hsp27 expression after KCl-induced ... [more ▼]

Spreading depression induces tolerance to ischemic injury, and ischemic tolerance has been associated with expression of heat shock proteins (Hsp). Here we examine Hsp27 expression after KCl-induced spreading depression. Twenty-minute cortical KCl application induced Hsp27 immunoreactivity in glial fibrillary acidic protein-positive astrocytes of the ipsilateral neocortex. Systemic administration of MK-801 (3 mg/kg) suppressed KCl-induced Hsp27 expression in the parietal cortex. Astrocytes in the posterior cingulate and retrosplenial cortex did not express Hsp27 after KCl application but did express Hsp27 after systemic administration of high dose MK-801 (9 mg/kg). Whereas Hsp27 was usually observed in all layers of the parietal cortex after 5-minute application of KCl, in 2 of 6 rats, Hsp27 was seen in clusters of astrocytes or in astrocytes in the superficial layers I to III of the parietal cortex. We conclude that (1) cortical application of KCl triggered Hsp27 astrocytic expression; (2) astrocytes in the cingulate and retrosplenial cortex responded differently compared with astrocytes of the parietal cortex; (3) Hsp27 expression progressed from small clusters of astrocytes throughout superficial layers of the cortex that joined and recruited astrocytes in deeper layers; (4) several mechanisms induced Hsp27 astrocytic expression. We propose that Hsp27 is involved in spreading depression-induced ischemic tolerance through protection of astrocyte function. [less ▲]

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See detailLes armées d'Alexandre le Grand et les langues étrangères
Rochette, Bruno ULg

in Antiquité Classique : Revue Interuniversitaire d'Etudes Classiques (1997), 66

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See detailLes strychnos africains et leurs alcaloïdes
Delaude, Clément ULg; Delaude, Lionel ULg

in Bulletin de la Société Royale des Sciences de Liège (1997), 66(4), 183-286

In the Greek Antiquity, the term strychnos was first coined to describe various toxic Solanacae. Nowadays, the genus Strychnos created by Linnaeus in 1753 is the numerically most important genus from the ... [more ▼]

In the Greek Antiquity, the term strychnos was first coined to describe various toxic Solanacae. Nowadays, the genus Strychnos created by Linnaeus in 1753 is the numerically most important genus from the Loganiaceae family and comprises 196 species growing in the warm regions of Asia (58 species), America (64) and Africa (75). Species on the three continents are almost totally segregated. Only Strychnos potatorum is common to Asia and Africa. The toxicity of Strychnos was empirically well-known from time immemorial. Inhabitants of South-Eastern Asia and India had an ancestral knowledge of Strychnos nux vomica, whose deadly poisonous seeds were later imported and marketed in Europe to kill rodents and small predators. Concentrated extracts from Amazonian Strychnos, known as curares, are also remarkable hunting poisons, used by South American Indians. Accordingly, modern scientific investigations of Strychnos and their alkaloids started with Asian or American species used as traditionnal poisons. Strychnine was the first alkaloid found in Strychnos. It was isolated from Asian S. nux vomica in 1818, but its structure was fully elucidated only in the 1950s. Meanwhile, various curarizing alkaloids from American Strychnos were identified. Based on these early results, Strychnos alkaloids were initially classified according to their geographical origin. Asian Strychnos were assumed to contain strychnine and related compounds, whereas curarizing ammonium salts were associated with American Strychnos. African species were assimilated to their Asian counterparts and deemed of less interest. During the last three decades, however, systematic screening of African Strychnos has revealed the presence of numerous, varied alkaloids in these plants and demonstrated that the correlation between geographical distribution and alkaloid content was erroneous. Presently, 47 African species have been investigated and their alkaloids analyzed, significantly more than Asian and American materials (about 10 and 20 species, respectively). In this review, we present the alkaloids isolated so far from African Strychnos. 309 structures are listed and sorted according to their molecular weight, the most useful index key in modern research based on mass spectrometry. [less ▲]

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See detailAcute Functional Iron Deficiency in Obese Subjects During a Very-Low-Energy All-Protein Diet
Beguin, Yves ULg; Grek, Vincent; Weber, Georges ULg et al

in American Journal of Clinical Nutrition (1997), 66(1), 75-9

We examined whether a very-low-energy all-protein diet (VLED) would produce detectable changes in iron as well as in other trace elements. Twenty-five obese patients consumed for 2 wk a VLED containing 70 ... [more ▼]

We examined whether a very-low-energy all-protein diet (VLED) would produce detectable changes in iron as well as in other trace elements. Twenty-five obese patients consumed for 2 wk a VLED containing 70 g protein after a 1-wk period during which total daily energy intake was progressively reduced to 1.26 MJ. Serum iron fell sharply by approximately equal to 50% (P < 0.0001), and despite a small decrease in total-iron-binding capacity, transferrin saturation decreased from 30 +/- 11% to 18 +/- 5% (P < 0.0001). Serum ferritin did not change significantly but serum soluble transferrin receptor (sTfR), an indicator of iron deficiency, increased progressively from 4630 +/- 1110 to 6070 +/- 1390 micrograms/L (P < 0.0001). Changes in sTfR correlated inversely with prior changes in serum iron. Changes in iron metabolism did not translate into changes in erythropoiesis or red cell indexes, but the white blood cell count decreased from 7.3 +/- 1.6 to 6.2 +/- 1.9 x 10(9)/L (P < 0.002). There was no evidence of deficiency for the other trace elements and minerals tested. Daily supplementation with 200 mg Fe in 18 other subjects only partially corrected these observations despite some increase in iron stores. These results indicate that during a 2-wk VLED serum iron is significantly depressed, inducing functional tissue iron deficiency too short in duration to produce alterations in red blood cell indexes. These changes are not mediated by absolute iron deficiency, inflammation, or protein malnutrition but could be related to alterations in the iron storage and release behavior of the reticuloendothelial cell during energy deprivation alone. [less ▲]

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See detailAntitumor and radiosensitizing effects of (E)-2'-Deoxy-2'-(Fluoromethylene) cytidine, a novel inhibior of ribonucleotide diphosphate reductase on human colon carcinoma xenografts in nude mice.
Sun, Lin-Quan; Li, Ye-Xiong; Guillou, Louis et al

in Cancer Research (1997), 57

Antitumor and radiosensitizing effects of (E).2'-deoxy.2'-(fluromethyl ene) cytidine (FMdC), a novel inhibitor of ribonucleotide reductase, were evaluated on nude mice bearing s.c. xenografts and liver ... [more ▼]

Antitumor and radiosensitizing effects of (E).2'-deoxy.2'-(fluromethyl ene) cytidine (FMdC), a novel inhibitor of ribonucleotide reductase, were evaluated on nude mice bearing s.c. xenografts and liver metastases of a human colon carcinoma. FMdC given once daily or twice weekly has a dose-dependent antitumor effect. The maximum tolerated dose In the mice was reached with 10 mgi'kg applied daily over 12 days. Twice weekly administration of FMdC reduced its toxicity but lowered the antitumor effect. Treatment of preestablished liver micrometastases obtained via intrasplenic injection of tumor cells, with 5 or 10 mgfkg FMdC, signifi candy prolonged the survival of the mice as compared to controls (P < 0.025 and P < 0.001, respectively). Ten mg/kg resulted in longer survival than S mg/kg FMdC (P < 0.05). Radiotherapy alone of s.c. xenografts (10 fractions over 12 days) yielded the radiation dose required to produce local tumor control in 50% of the treated mice (TCD@O)of 43.0 Gy. When combined with FMdC, TCDsawas reduced to 22.5 and 19.0 Gy at doses of 5 and 10 mg/kg given i.p. 1 h before each irradiation, respec tively. The corresponding enhancement ratios were 1.91 and 2.43, respec lively. FMdC produced moderate and reversible myelosuppression. When 5 mg/kg FMdC was combined with irradiation, there was no increased skin or hematological toxicity as compared to radiotherapy or FMdC alone. At the 10 mg/kg level, however, lower leukocyte counts were observed. These results show that FMdC appears to be a potent anticancer drug and radiosensitizer [less ▲]

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See detailRoma ou Ruma ?
Rochette, Bruno ULg

in Maia (1997), 49

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See detailPhysical activity, obesity, and cardiovascular risk factors in children. The Belgian Luxembourg Child Study II.
Guillaume, Michèle ULg; Lapidus, L.; Bjondorp, P. et al

in Obesity Research (1997), 5(6), 549-56

Physical activity was measured in relation to cardiovascular (CV) risk factors in a randomly selected population of 1028 children from Province de Luxembourg in Belgium, a mainly rural area with a high ... [more ▼]

Physical activity was measured in relation to cardiovascular (CV) risk factors in a randomly selected population of 1028 children from Province de Luxembourg in Belgium, a mainly rural area with a high prevalence of such risk factors among adults and children. Physical activity was estimated as participation in sport activities, a major indicator of leisure-time physical activity in schoolchildren, and physical inactivity was estimated as frequency and duration of television (TV) watching. Boys participated more frequently in sport activities than girls did (p = 0.001). A majority of the children watched TV daily. After age adjustment, bodyweight (girls, p < 0.012; boys, p < 0.027) and, in boys, body mass index (BMI) (p < 0.039) were related to days per week of TV watching. No significant relationships with other CV risk factors remained after adjustments for BMI. In analyses of independent contributions of age, TV watching, and sports activity on CV risk factors, age showed highly significant relationships. In boys, TV showed relationships with BMI (p < 0.04) and (borderline) with systolic blood pressure, independent of age and sports activity, whereas the latter was significantly related to subscapular skinfold (p < 0.04) and (borderline) with triceps skinfold and cholesterol. In girls, no significant independent contributions to risk factor associations were found. The father's education was directly associated with sports activities, whereas the mother being a housewife showed negative relationships to physical activity and positive to TV watching in their children, suggesting socioeconomic influence on the activity patterns of children. Furthermore, registrations suggested less physical activity in the most rural part of the area. It is concluded that children in this mainly rural area watch TV frequently. In boys, physical inactivity, measured both as TV watching and as registrations of sports activities, contributes independently to body fat mass. In girls, no contribution or weaker contributions of physical inactivity were found. This suggests that contributory factors leading to obesity might be different in girls and boys. [less ▲]

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See detailCharacterization of non pigmented B16 melanoma cell-derived cytotoxic factors.
Siwek, B.; De Pauw-Gillet, Marie-Claire ULg; Quetin-Leclercq, J. et al

in Chemico-Biological Interactions (1997), 103(1), 59-73

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests ... [more ▼]

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests used showed that ultrafiltrated conditioned media (CM U1 fraction) contained several cytotoxic factors with a Mw lower than 1000 Da. These factors seemed to act either directly or indirectly on cell membranes, mitochondria, on the cell cycle and on protein and DNA synthesis. A cytotoxic activity could be found even after high dilution of CM U1. These cytotoxic factors were rapidly released by B16 cells in culture, independently of cell confluence. Their activities in the treated cells were also very fast and the cytotoxic effects were irreversible after only a few hours of treatment. These factors were not intermediate products during melanogenesis, neither polyamines, nor proteases. At least one of them seemed to be a small acidic and basic stable peptide without disulfide bounds but not heat stable. The synthesis of at least one of these cytotoxic factors was inhibited by cycloheximide and the cytotoxic activity was partially destroyed by pronase and trypsin, but not by pepsin. The cytotoxicity was not modified by copper complexants or free radical inhibitors (bovine serum albumin (BSA), tyrosine, superoxyde dismutase (SOD), catalase, vitamin E). Furthermore the levels of glutathione peroxydase activity and reduced glutathione did not change after treatment by CM U1 as compared to controls. [less ▲]

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See detailLoss of calcyphosin gene expression in mouse and other rodents
Clément, S.; Dumont, J.; Schurmans, Stéphane ULg

in Biochemical and Biophysical Research Communications (1997), 232

alcyphosine-for calcium binding and regulated by cyclic AMP through phosphorylation protein-is a target of both the cyclic AMP and the Ca(+2)-phophatidylinositol cascades first isolated from dog thyroid ... [more ▼]

alcyphosine-for calcium binding and regulated by cyclic AMP through phosphorylation protein-is a target of both the cyclic AMP and the Ca(+2)-phophatidylinositol cascades first isolated from dog thyroid, and then from rabbit and human brain. Although the exact function of this 24kD protein is unknown, calcyphosine could be implicated in the cross-signaling between these cascades to coordinate cellular proliferation and differentiation. Here, we report the sequence of a pseudogene which is the murine calcyphosine homologue, and demonstrate that it represents the unique sequence homologous to the dog calcyphosine gene in the murine genome. The lack of expression of this murine pseudogene in brain and thyroid-two major sites of dog calcyphosine expression-was extended to 5 other rodents, and suggest the existence of alternative pathway(s) to fill the function of calcyphosine in rodents [less ▲]

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See detailRadiotherapie externe focalisee apres resection de metastase cerebrale unique: etude prospective de phase I-II
Zouhair; COUCKE, Philippe ULg; Douglas, Pelham et al

in Bulletin du Cancer. Radiothérapie : Journal de la Société Française du Cancer : Organe de la Société Française de Radiothérapie Oncologique (1997), 1

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See detailWhat Is the Risk of Transferral of an Undetected Neoplasm During Organ Transplantation?
Detry, Olivier ULg; Bonnet, Pierre ULg; Honore, Pierre ULg et al

in Transplantation Proceedings (1997), 29(5), 2410-1

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See detailTerm analysis and Hyperfine Structure in Neutral Vanadium
Palmeri, P.; Biémont, Emile ULg; Quinet, P. et al

in Physica Scripta (1997), 55

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See detailSpecific repertoire of olfactory receptor genes in the male germ cells of several mammalian species
Vanderhaeghen, P.; Schurmans, Stéphane ULg; Vassart, G. et al

in Genomics (1997), 39

Olfactory receptors constitute the largest family among G protein-coupled receptors, with up to 1000 members expected. We have previously shown that genes belonging to this family were expressed in the ... [more ▼]

Olfactory receptors constitute the largest family among G protein-coupled receptors, with up to 1000 members expected. We have previously shown that genes belonging to this family were expressed in the male germ line from both dog and human. We have subsequently demonstrated the presence of one of the corresponding olfactory receptor proteins during dog spermatogenesis and in mature sperm cells. In this study, we investigated whether the unexpected pattern of expression of olfactory receptors in the male germ line was conserved in other mammalian species. Using reverse transcription-PCR with primers specific for the olfactory receptor gene family, about 20 olfactory receptor cDNA fragments were cloned from the testis of each mammalian species tested. As a whole, they displayed no sequence specificity compared to other olfactory receptors, but highly homologous, possibly orthologous, genes were amplified from different species. Finally, their pattern of expression, as determined by RNase protection assay, revealed that many but not all of these receptors were expressed predominantly in testis. The male germ line from each mammalian species tested ins thus characterized by a specific repertoire of olfactory receptors, which display a pattern of expression suggestive of their potential implication in the control of sperm maturation, migration, or fertilization [less ▲]

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See detailLat. collatrare
Rochette, Bruno ULg

in Latomus : Revue d'Etudes Latines (1997), 56

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See detailUltrastructural nucleolar alterations induced by an ametantrone/polyr(A-U) complex.
Thiry, Marc ULg; Jamison, J. M.; Gilloteaux, J. et al

in Experimental Cell Research (1997), 236(1), 275-84

In the present study we examined the ultrastructural modifications as well as the precise distribution of DNA and RNA in RT4 cell nucleoli following a 3-h exposure to nontoxic or toxic doses of ... [more ▼]

In the present study we examined the ultrastructural modifications as well as the precise distribution of DNA and RNA in RT4 cell nucleoli following a 3-h exposure to nontoxic or toxic doses of ametantrone (AMT), poly(adenylate-uridylate) (polyr(A-U), or an AMT/polyr(A-U) combination. While distribution of nucleic acids within the various nucleolar components is not modified following all treatments, the nucleoli exhibit several ultrastructural changes: redistribution of the nucleolar components, decrease in the number of fibrillar centers, and increase in the size of the fibrillar centers. The relative frequencies of the test agents to induce the apparition of nucleoli of compact type are AMT/polyr(A-U) > AMT approximately polyr(A-U) > sham-treated, while the abilities of the test agents to induce the nucleolar segregation are AMT/polyr(A-U) approximately AMT > polyr(A-U) > sham-treated cells. These ultrastructural changes are characteristics of drugs that intercalate into DNA and inhibit rDNA transcription as well as rRNA processing and release of nascent preribosomes from the nucleolus. [less ▲]

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See detailSympathetic modulation of hypoxic pulmonary vasoconstriction in intact dogs
Brimioulle, Serge; Vachiéry, Jean-Luc; Brichant, Jean-François ULg et al

in Cardiovascular Research (1997), 34

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See detailVision artificielle et quantification de la forme de pommes
Leemans, Vincent ULg; Magein, Hugo ULg; Destain, Marie-France ULg

in Cahiers Agricultures (1997), 6

Golden delicious apples were characterised automatically by machine vision, taking into account six view of the cheeks and one of the stem end. The Fourier transform of the fruit boundaries were computed ... [more ▼]

Golden delicious apples were characterised automatically by machine vision, taking into account six view of the cheeks and one of the stem end. The Fourier transform of the fruit boundaries were computed and normalised. All the relevant information about the shape was included in the first fifteen harmonics. Moreover, particular harmonics were found to characterise the shape of the Golden delicious variety. By using the amplitude of particular harmonics in a discriminant analysis, apple were sorted into categories defined by EU, with a precision around 96%. The proposed method may also be used to compare varieties objectively, in order to identify relation between physiology and shape. [less ▲]

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See detailFrom an architectural sketch to its semantic representation
Leclercq, Pierre ULg

in International Journal of Construction Information Technology (1997), 4(2), 67-84

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See detailLe bois de tension. 1 Définition et distribution dans l'arbre.
Jourez, Benoît ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1997), 1(2), 100-112

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See detailPrimary radiation therapy or surgery combined or not to radiation therapy in the management of squamous cell carcinoma of the penis
Zouhair, Abderrahim; Ozsahin, Mahmut; Douglas, Pelham et al

in International Journal of Radiation, Oncology, Biology, Physics (1997), 39(2 (Supplément)), 295

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See detailThe intensity of emotional facial expression and decoding accuracy
Hess, U; Blairy, Sylvie ULg; Kleck, R

in Journal of Nonverbal Behavior (1997), 21

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See detailA new generation of hydrocolloid dressings in combination with topical corticosteroids in the treatment of psoriasis vulgaris
Dezfoulian, Bita ULg; de LA BRASSINNE, Michel ULg; Rosillon, D.

in Journal of the European Academy of Dermatology & Venereology (1997), 9

Aim : A clinical, randomized, parallel, multicenter study was undertaken to compare the efficacy and Ihe tolerability of two known steroid creams, betamethasone 17-valerate and clobetasol propionate ... [more ▼]

Aim : A clinical, randomized, parallel, multicenter study was undertaken to compare the efficacy and Ihe tolerability of two known steroid creams, betamethasone 17-valerate and clobetasol propionate, alone or left under a hydrocolloid dressing (Contreei'* Derma Cover), in the treatment of chronic plaques of psoriasis. Methods : A total of 70 patients with symmetrical localized psoriasis were divided randomly into two groups for the study. Each patient applied ihe corticosteroid preparation under Contreet° Derma Cover once a week on one plaque and the same topical corticosteroid alone h.i.d on the other plaque; the treatment lasted 3 weeks for betamethasone 17-valerate, and 2 weeks for clobetasol propionate. Results : The heahng rate reached 79% with the Contreet" combined treatment versus 15% (P < 0,0001) with the standard treatment for betamethasone 17-valeratc (group I) and Sb% versus 14*70 (P < 0. 0001) for clobetasol propionate (group It). The mean difference in healing rate was 65% {95% confidence interval (C.I), 46-84%) and 69% (95% CL, 50-87%) in groups I and II, respectively. Conclusion : The use of the new generation of hydrocolloid dressings in combination with steroid preparations can be considered as another therapeutic option for the treatment of chronic localized psoriatic lesions. Furthermore, with this method the amount of the topical corticosteroid used can be reduced. [less ▲]

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See detailImaging the solar corona in the EUV
Delaboudiniere, J.-P.; Stern, R. A.; Maucherat, A. et al

in Advances in Space Research (1997), 20

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the ... [more ▼]

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the Sun. As one of the instruments onboard SOHO, the EIT (Extreme ultraviolet Imaging Telescope) images the Sun's corona in 4 EUV wavelengths. The He II filter at 304 AË images the chromosphere and the base of the transition region at a temperature of 5 - 8 x 10^4 K; the Fe IX-X filter at 171 AË images the corona at a temperature of ~ 1.3 x 10^6 K; the Fe XII filter at 195 AË images the quiet corona outside coronal holes at a temperature of ~ 1.6 x 10^6 K; and the Fe XV filter at 284 AË images active regions with a temperature of ~ 2.0 x 10^6 K. About 5000 images have been obtained up to the present. In this paper, we describe also some aspects of the telescope and the detector performance for application in the observations. Images and movies of all the wavelengths allow a look at different phenomena present in the Sun's corona, and in particular, magnetic field reconnection. [less ▲]

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See detailEndoscopic laser treatment of uterine malformations.
Donnez, Jacques; NISOLLE, Michelle ULg

in Human Reproduction (1997), 12(7), 1381-7

Hysteroscopic resection of an intrauterine septum may benefit patients suffering from infertility or recurrent pregnancy loss. A partial or complete uterine septum can be easily resected using a Nd-YAG ... [more ▼]

Hysteroscopic resection of an intrauterine septum may benefit patients suffering from infertility or recurrent pregnancy loss. A partial or complete uterine septum can be easily resected using a Nd-YAG laser. If present, the vaginal septum may also be removed during the same procedure. The reproductive outcome of women treated by operative hysteroscopy for an intrauterine septum is reviewed. To avoid pregnancy in a non-communicating rudimentary horn, the removal of the horn and the homolateral tube may be performed by either bipolar coagulation or a CO2 laser. [less ▲]

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See detailReprésentation de l'espace ouvert dans un système d'information de projet urbain
Dupagne, A.; Teller, Jacques ULg

in Ingénierie des Systèmes d'Information (1997), 5(2), 219-240

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See detailLa cavernostomie: une ancienne technique parfois efficace dans le traitement des abcès pulmonaires
Defraigne, Jean-Olivier ULg; Siquet, J.; Limet, Raymond ULg

in Revue Médicale de Liège (1997), 52(7), 498-501

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See detailExpression of interstitial collagenase (matrix metalloproteinase-1) is related to the activity of human endometriotic lesions
Kokorine, Isabelle; NISOLLE, Michelle ULg; Donnez, Jacques et al

in Fertility and Sterility (1997), 68(2), 246-251

Objective: To determine whether interstitial collagenase (matrix metalloproteinase-1), known to play a pivotal role in the initiation of menstruation, contributes to the pathogenesis of endometriosis ... [more ▼]

Objective: To determine whether interstitial collagenase (matrix metalloproteinase-1), known to play a pivotal role in the initiation of menstruation, contributes to the pathogenesis of endometriosis. Design: Serial sections of peritoneal red and black endometriotic lesions, ovarian endometriotic cysts, and rectovaginal adenomyotic nodules were analyzed by in situ hybridization for the expression of matrix metalloproteinase-1 by silver staining for the integrity of the fibrillar extracellular matrix and by immunolabeling for the abundance of sex steroid receptors. Setting: Academic hospital and research laboratory. Patient(s): Premenopausal women undergoing laparoscopy for endometriosis. Intervention(s): Biopsy of endometriotic lesions, combined with endometrium whenever possible. Main Outcome Measure(s): Expression of matrix metalloproteinase-1 messenger RNA (mRNA). Result(s): Matrix metalloproteinase-1 mRNA was expressed focally in red peritoneal and ovarian endometriosis irrespective of the phase of the menstrual cycle but was not detectable in black peritoneal and rectovaginal lesions. Foci of matrix metalloproteinase-1 expression closely correlated with matrix breakdown and with the absence of P receptors in adjacent epithelial cells. Conclusion(s): Correlation of matrix metalloproteinase-1 expression with activity of endometriotic tissue suggests its involvement in tissue remodeling and bleeding, and possibly in the secondary shedding and reimplantation of endometriotic lesions. [less ▲]

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See detailLe maintien des droits aux prestations de santé lors d'une séparation de fait ou de corps
Michiels, Olivier ULg

in Divorce : Actualité Juridique, Sociale et Fiscale (1997)

L'article examine la question du maintien des droits aux prestations de santé lors d'une séparation de corps ou d'une séparation de fait.

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See detailSynthesis and characterization of porous silica-alumina xerogels
VanCantfort, Olivier; Abid, A.; Michaux, Bernard et al

in Journal of Sol-Gel Science and Technology (1997), 8

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See detailThe Penicillin sensory transducer, blar, involved in the inducibility of beta-lactamase synthesis in bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle
Hardt, Karin; Joris, Bernard ULg; Lepage, Sophie et al

in Molecular Microbiology (1997), 23(5), 935-944

Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in ... [more ▼]

Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis, is embedded in the plasma membrane bilayer via four transmembrane segments TM1-TM4 that forma four-alpha-helix bundle. The extracellular 262-amino-acid-residue polypeptide, S340-R601, that is fused at the carboxy end of TM4, possesses the amino acid sequence signature of a penicilloyl serine transferase. It probably functions as penicillin sensor. As an independent entity, this polypeptide behaves as a high-affinity penicillin-binding protein. As a component of the full-size BlaR, it adopts a different conformation presumably because of interactions with the extracellular 63-amino-acid-residue P53-S115 loop that connects TM2 and TM3. Reception of the penicillin-induced signal requires a precise conformation of the sensor but it does not involve penicilloylation of the serine residue S402 of motif STYK. Signal transmission through the plasma membrane by the four-alpha-helix bundle may proceed in a way comparable to that of the aspartate receptor, Tar. Signal emission in the cytosol by the intracellular 189-amino-acid-residue Y134-K322 loop that connects TM3 and TM4, may proceed via the activation of a putative metallopeptidase. [less ▲]

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