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See detailMiddle and Upper Devonian Miospore Zonation of Eastern Europe.
Avkhimovitch, V.I.; Tchibrikova, E.V.; Obukhovskaya, T.G. et al

in Bull. Centres Rech. Explor.-Prod. Elf Aquitaine, (1993), 17

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See detailEvolutionary relationships among higher fungi inferred from small ribosomal subunit RNA sequence analysis
Wilmotte, Annick ULg; Van De Peer, Yves; Goris, Anne et al

in Systematic & Applied Microbiology (1993), 16

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The ... [more ▼]

The primary structure of the small ribosomal subunit RNA (SSU rRNA) was determined for 13 species belonging to 10 ascomycete families and for the basidiomycetous anamorphic yeast Rhodotorula glutinis. The sequences were fitted into an alignment of all hitherto published complete or nearly complete eukaryotic small subunit rRNA sequences. The evolutionary relationships within the fungi were examined by construction of a tree from 87 SSU rRNA sequences, corresponding to 71 different species, by means of a distance matrix method and bootstrap analysis. It confirms the early divergence of the zygomycetes and the classical division of the higher fungi into basidiomycetes and ascomycetes. The basidiomycetes are divided into true basidiomycetes and ustomycetes. Within the ascomycetes, the major subdivisions hemiascomycetes and euascomycetes can be recognized. However, Schizosaccharomyces pombe does not belong to the cluster of the hemiascomycetes, to which it is assigned in classical taxonomic schemes, but forms a distinct lineage. Among the euascomycetes, the plectomycetes and the pyrenomycetes can be distinguished. Within the hemiascomycetes, the polyphyly of genera like Pichia or Candida and of families like the Dipodascaceae and the Saccharomycetaceae can be observed. [less ▲]

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See detailRestriction Maps of the DNA of Cervid Herpesvirus 1 and Cervid Herpesvirus 2, Two Viruses Related to Bovine Herpesvirus 1
Vanderplasschen, Alain ULg; Bublot, M.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 128(3-4), 379-88

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as ... [more ▼]

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. [less ▲]

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See detailLe traitement des adénomes à prolactine : utilisation d'un nouvel agoniste dopaminergique à longue durée d'action, la cabergoline, chez 56 patients
Beckers, Albert ULg; Abs, R.; Demez, Pierre ULg et al

in Médecine et Hygiène (1993), 51(1990), 1978-1980

Nous avons utilisé la cabergoline, un nouvel agoniste dopaminergique, dans le traitement des adénomes hypophysaires à prolactine. Cinquante-six patients atteints de micro-adénome dans 35 cas et de ... [more ▼]

Nous avons utilisé la cabergoline, un nouvel agoniste dopaminergique, dans le traitement des adénomes hypophysaires à prolactine. Cinquante-six patients atteints de micro-adénome dans 35 cas et de macroadénome dans 21 cas ont été traités durant des périodes variant entre 1 et 17 mois à des doses de cabergoline variant entre 0,25 mg et 2 mg par semaine, prises à raison de 1 à 4 fois par semaine. Cette nouvelle substance nous est apparue extrêmement efficace et très bien tolérée [less ▲]

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See detailPalynological data from the Devono-Carboniferous boundary beds in the new Stockum Trench II and the Hasselbachtal Borehole. Northern Rhenish Massif, Germany.
Higgs, K; Streel, Maurice ULg; Korn, D et al

in Annales de la Société Géologique de Belgique (1993), 115(2/1992), 551-557

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See detailMourir à son postmoderne
Demoulin, Laurent ULg

in Ecritures (1993), 5

Treize réflexions sur la notion de postmodernité, considérée étymologiquement, c'est-à-dire comme un "après" la modernité (et non comme un retour en arrière, ou une surmodernité). La postmodernité est à ... [more ▼]

Treize réflexions sur la notion de postmodernité, considérée étymologiquement, c'est-à-dire comme un "après" la modernité (et non comme un retour en arrière, ou une surmodernité). La postmodernité est à distinguer du postmodernisme (si celui-ci existe). [less ▲]

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See detailStructure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution
Noble, M. E.; Zeelen, J. P.; Wierenga, R. K. et al

in Acta Crystallographica Section D-Biological Crystallography (1993), 49(Pt 4), 403-17

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 ... [more ▼]

The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 A resolution with a crystal grown by the technique of hanging-drop crystallization from a mother liquor containing the transition-state analogue 2-phosphoglycolate (2PG). As a search model in the molecular replacement calculations, the refined structure of TIM from Trypanosoma brucei, which has a sequence identity of 46% compared to the enzyme from E. coli, was used. An E. coli TIM crystal grown in the absence of 2PG, diffracting to 2.6 A resolution, was later obtained by application of the technique of macro-seeding using a seed crystal grown from a mother liquor without 2PG. The final 2.6 A model has a crystallographic R factor of 11.9%, and agrees well with standard stereochemical parameters. The structure of E. coli TIM suggests the importance of residues which favour helix initiation for the formation of the TIM fold. In addition, TIM from E. coli shows peculiarities in its dimer interface, and in the packing of core residues within the beta-barrel. [less ▲]

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See detailTransmission du virus BVD/MD et aspects épidémiologiques de l'infection
Boulanger, D.; Quatpers, D.; Mignon, Bernard ULg et al

in Bulletin des Groupements Techniques Vétérinaires (1993), 4

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See detailPalynological age for the lower part of the Hangenberg Shales in Sauerland, Germany
Higgs, K; Streel, Maurice ULg

in Annales de la Société Géologique de Belgique (1993), 116(Z), 243-247

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See detailLog jam effect on bed-load mobility from experiments conducted in a small gravel-bed channel
Assani, Ali; Petit, François ULg

in Memorial symposium Pro. J. De Ploey Experimental geomorphology and landscape ecosystem changes (1993)

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See detailComparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo
Ectors, Fabien ULg; Thonon, Fabienne; Delval, Alain et al

in Livestock Production Science (1993), 36

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC ... [more ▼]

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC conditioned medium (CM) or in rabbit oviducts, versus in vivo produced embryos. There was no significant difference in terms of percentages of cleaved and 8-cell stages obtained between CM and BOEC. In CM, 24.8% of the cleaved embryos became blastocysts. In BOEC, 14.5% of the cleaved embryos became blastocysts. Among the 190 zygotes transferred in the rabbit oviducts, 127 have been recovered 5 days later, and 17.4% became blastocysts. There was no significant difference in term of blastocyst formation between the development in rabbit and in BOEC. However, there was a significant difference between the CM group and the two other groups. The numbers of cells in blastocysts from different sources were investigated: in vivo blastocysts contained 107 cells assumed to be 100%, in vitro blastocysts developed in rabbit oviduct 100.1 cells (93.3%), BOEC blastocysts 90.8 cells (84.6%) and CM blastocysts 72.3 cells (67.3%). This study confirmed earlier works on the oviduct effect on blastocyst quality in terms of development rate and cell number. [less ▲]

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See detailPeut-on réfléchir sereinement sur les migrations humaines ?
Martiniello, Marco ULg

in Agenda Interculturel (1993), 113

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See detailClonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(6), 427-431

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See detailEmperipolesis of lymphoid cells by human follicular dendritic cells in vitro.
Tsunoda, R.; Nakayama, M.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailL'apprentissage précoce des langues étrangères par la méthode 'immersive'
Comblain, Annick ULg; Rondal, Jean-Adolphe ULg

in Rééducation Orthophonique (1993), 31

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See detailC.R. de S. KAY, Subjectivity in Troubadour Poetry
Henrard, Nadine ULg

in Moyen Age (Le) (1993), IC

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See detailOn the Use of Distance in the Taxonomic Study of Critical Plant Groups-Case Studies of Western European Orchidaceae
Tyteca, D.; Dufrêne, Marc ULg

in Annals of Botany (1993), 71(3), 257-277

Four distance coefficients are compared on four data sets composed of samples coming from western European populations of the genera Dactylorhiza, Orchis and Epipactis (Orchidaceae). The performance of ... [more ▼]

Four distance coefficients are compared on four data sets composed of samples coming from western European populations of the genera Dactylorhiza, Orchis and Epipactis (Orchidaceae). The performance of the distance coefficients is evaluated through: (a) the quality of clusters obtained by five classical methods (as compared to a priori classification), (b) the Mantel statistic with respect to an a priori distance matrix resulting from previous knowledge, (c) the result obtained with the k-means method, and (d) principal coordinate diagrams. It appears that: (a) the Mahalanobis distance based on the pooled dispersion matrix performs best on the whole; (b) a distance based on the recently developed Common Principal Component model, used with a log transformation, also provides useful information and performs best on the largest data set; (c) the Gölz and Reinhard taxonomic distance, widely used among orchidologists, is attractive for its simplicity, yet good performance and the valuable information it provides, despite its theoretical shortcomings. A brief taxonomic discussion is made on the results obtained for the Dactylorhiza samples, especially about samples whose identification was in doubt. [less ▲]

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See detailFollicular Dendritic Cells Do Not Produce Tnf-Alpha nor its Receptor
Mancini, I.; Bosseloir, A.; Hooghe-Peters, E. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailCloning and characterization of G protein-coupled receptors
Parmentier, M.; Libert, F.; Perret, J. et al

in Advances in Second Messenger and Phosphoprotein Research (1993), 28

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See detailLa myocardiopathie hypertrophique obstructive: options therapeutiques chirurgicales.
Radermecker, Marc ULg; SAKALIHASAN, Natzi ULg; Partoune, B. et al

in Revue Médicale de Liège (1993), 48(12), 659-65

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See detailTransforming growth factor ß as a neuronoglial signal during peripheral nervous sytem response to injury.
Rogister, Bernard ULg; Delrée, P.; Leprince, Pierre ULg et al

in Journal of Neuroscience Research (1993), 34

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See detailAccelerated erythroid repopulation with no stem-cell competition effect in children treated with recombinant human erythropoietin after allogeneic bone marrow transplantation.
Locatelli, F.; Zecca, M.; Beguin, Yves ULg et al

in British Journal of Haematology (1993), 84(4), 752-4

We carried out a pilot study on the use of recombinant human erythropoietin (rHuEPO) to accelerate erythropoietic engraftment in paediatric patients undergoing allogeneic BMT. rHuEPO was administered ... [more ▼]

We carried out a pilot study on the use of recombinant human erythropoietin (rHuEPO) to accelerate erythropoietic engraftment in paediatric patients undergoing allogeneic BMT. rHuEPO was administered intravenously at a dose of 75 U/kg per day for 30 d after transplant. Erythroid repopulation, evaluated sequentially through the serum transferrin receptor, was faster in 15 patients receiving rHuEPO than in 16 historical controls (P = 0.0003). This faster erythroid engraftment resulted in a reduction in the total number of red blood cell units required to reach transfusion independence (2.7 +/- 1.2 v 4.2 +/- 2.3, P = 0.027). No significant difference in leucocyte or platelet regeneration was observed. These findings indicate that rHuEPO administration can accelerate erythroid recovery after allogeneic BMT and reduce red cell transfusion requirements with no stem-cell competition effect. [less ▲]

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See detailStructure sociologique de l'élecotrat écologiste en Wallonie : une première exploration
Jacquemain, Marc ULg; Vandekeere, Michel; Doutrelepont, René

in Res Publica (1993), 25

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much ... [more ▼]

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much more educated, e.a. [less ▲]

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See detailDecision tree approaches to voltage security assessment
Van Cutsem, Thierry ULg; Wehenkel, Louis ULg; Pavella, Mania ULg et al

in IEE Proceedings - Generation, Transmission & Distribution (1993), 140

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See detailPlasticity of Developing and Adult Dorsal Root Ganglion Neurons as Revealed in Vitro
Delree, P.; Ribbens, Clio ULg; Martin, Didier ULg et al

in Brain Research Bulletin (1993), 30(3-4), 231-7

We review recent data on the plasticity of dorsal root ganglion (DRG) neurons as revealed during cultivation in vitro. Some experiments on cultured developing DRG neurons and on adult DRG neurons in vivo ... [more ▼]

We review recent data on the plasticity of dorsal root ganglion (DRG) neurons as revealed during cultivation in vitro. Some experiments on cultured developing DRG neurons and on adult DRG neurons in vivo are also mentioned. Cultured developing and adult DRG neurons can be switched from an apolar to a multipolar phenotype by fetal calf serum or fibronectin. The effect is concentration dependent and occurs through an early modification of cell-substratum interaction. Adult DRG neurons synthesize and release within hours after injury TGF beta-1, which is a mitogen and a differentiation factor for Schwann cells. Finally, adult DRG neurons express in vitro neurotransmitters that are not expressed in vivo. This neurotransmitter plasticity can be modulated in vitro by some growth factors and in vivo by distal or proximal axotomy. [less ▲]

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See detailQuantitative estimation of chimerism in mice using microsatellite markers
Delhaise, F.; Zhao, X.; Dessy, F. et al

in Molecular Reproduction and Development (1993), 34(2), 127-132

An embryonic stem cell line was established from SV129 mouse blastocysts and used to generate chimeric mice by injection into OF1 blastocysts; 18 out of the 30 resulting offspring appeared chimeric as ... [more ▼]

An embryonic stem cell line was established from SV129 mouse blastocysts and used to generate chimeric mice by injection into OF1 blastocysts; 18 out of the 30 resulting offspring appeared chimeric as judged from their coat color patterns, and 3 of the 13 males proved to be germ-line chimeras as they transmitted the SV129 agouti phenotype to all or part of their offspring. The degree of chimerism of these males was evaluated for different tissues using polymorphic microsatellite markers amplified by the polymerase chain reaction. It was shown that these new markers can be effectively used to quantitatively estimate levels of chimerism. The CKMM (creatine kinase, muscle) microsatellite system was used to distinguish the SV129 from the OF1 genotype. In all performed tests, the correlation between DNA ratio and signal ratio, expressed as a base 10 logarithm, was shown to exceed or equal 0.98 for known DNA ratios (SV129/OF1) ranging from 1/99 to 99/1. Linear calibration methods were used to predict the % SV129 DNA of a test sample based on the obtained signal ratio. The accuracy of the prediction was evaluated by performing repeated measurements. Differences among three repeated estimates ranged from 2 to 17% for a given sample. Microsatellite systems should be very useful to monitor chimerism involving strains that can not be discerned with coat color or biochemical markers. This will be particularly important when ES methodology becomes available in species other than mice. [less ▲]

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See detailExteroceptive suppression of temporalis muscle activity in patients with chronic headache and in normal volunteers: methodology, clinical and pathophysiological relevance.
Schoenen, Jean ULg

in Headache (1993), 33(1), 3-17

Exteroceptive suppression of temporalis muscle activity was studied in patients with chronic headache and in healthy controls. Among different methods of recording, averaging 10 full-wave rectified EMG ... [more ▼]

Exteroceptive suppression of temporalis muscle activity was studied in patients with chronic headache and in healthy controls. Among different methods of recording, averaging 10 full-wave rectified EMG responses produces results with acceptable variability and discomfort. The late temporalis exteroceptive suppression period (ES2) is reduced on average in patients with chronic tension-type headache; this finding has been reproduced by several independent laboratories. Mean duration of temporalis ES2 is also diminished, but to a lesser degree, in daily drug abuse headache and, as shown by others, in episodic tension-type headache. It is normal in migraine between attacks, cluster headache and various types of symptomatic headaches. Temporalis ES2 may be decreased in untreated patients with major depression. In healthy volunteers, temporalis ES2 duration is reduced by a short-lasting painful stimulus to peripheral limbs after a delay of 50 to 60 ms, and by a sub-motor threshold electromagnetic stimulation to the contralateral cerebral cortex after a delay of 20 to 30 ms. In contrast, long-lasting trains of peripheral painful stimuli have no effect. Various pharmacological agents are able to modify temporalis ES2. Its duration is increased by 5-HT antagonists, but decreased by 5-HT uptake blockers. Pharmacological effects may differ between controls and patients. Considering these results and available data on the anatomo-functional organization of masticatory reflexes, we postulate that temporalis ES2 is a marker of the excitability of interneuronal nets in the ponto-medullary reticular formation. In chronic tension-type headache, excitability of these interneurons is decreased because of inadequate control by the serotonergic raphe magnus nucleus and the periaqueductal gray matter. Dysfunctioning of the latter structures might be caused by abnormal limbic inputs to the brain stem. Some steps of this pathophysiological hypothesis can be verified by modern neurophysiological techniques. [less ▲]

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See detailUse of an antisense oligonucleotide to inhibit expression of a mutated human procollagen gene (COL1A1) in transfected mouse 3T3 cells.
Colige, Alain ULg; Sokolov, B. P.; Nugent, P. et al

in Biochemistry (1993), 32(1), 7-11

A series of antisense oligonucleotides were developed to inhibit specifically expression of a mutated exogenous gene for collagen without inhibiting expression of an endogenous gene for the same protein ... [more ▼]

A series of antisense oligonucleotides were developed to inhibit specifically expression of a mutated exogenous gene for collagen without inhibiting expression of an endogenous gene for the same protein. The test system consisted of mouse NIH 3T3 cells that were stably transfected with an internally deleted construct of the human gene for the pro alpha 1(I) chain of type I procollagen [Olsen et al. (1991) J. Biol. Chem. 266, 1117]. The target site was a region at the 3' end of exon 1 and the first few nucleotides of intron 1 of the exogenous human gene that differed in sequence by nine nucleotides from the sequence of the endogenous mouse gene. Expression of the two genes was assayed by Western blot with cross-reacting antibodies and by steady-state levels of mRNAs. None of the oligonucleotides were effective in concentrations up to 25 microM when administered without any carrier. However, when administered with 5 or 10 micrograms/mL lipofectin, one of the oligonucleotides in concentrations of 0.1-0.2 microM inhibited expression of the exogenous gene from 50% to 80% without significant inhibition of expression of the endogenous gene. Also, a missense version of the same oligonucleotide had no significant effect, and the inhibition observed with the most effective oligonucleotide was abolished by a single base change. Time course experiments indicated that, after a 4-h treatment, inhibition appeared at 8 h and persisted for at least 22 h.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailDynamics of mitotic activity and expression of viral proteins gp51 and p24 of Bovine Leukemia Virus producing cells.
Roussev, Roussi; Polianova, M.; Portetelle, Daniel ULg et al

in Folia Biologica (1993), 39

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See detailSmall Bowel Enema and Diagnosis of Chronic Nonischemic Disturbance of Superior Mesenteric Venous Blood Flow
Boverie, J. H.; COUNET, D.; Meunier, Paul ULg et al

in Abdominal Imaging (1993), 18(3), 265-70

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis ... [more ▼]

Chronic nonischemic disturbance of mesenteric venous blood flow is reported in 11 patients with a mean age of 19 years. This entity, rarely discussed in the literature, is different from acute thrombosis and chronic thrombotic forms with portal hypertension or hypercoagulopathy. In eight patients this syndrome was secondary to organic lesions of different origin: mesenteric vein squeezed by fibrous bands or an abnormal jejunal artery (four cases), lymphoma involving the distal superior mesenteric veins (three cases), hemangioma causing microthrombi (one case). In three patients no etiology or predisposing factor was found. All patients presented with rectal hemorrhage. Small bowel enema showed a constant pattern in 11 patients: small nodules, modified by compression or peristalsis, involving the mesenteric border of the jejunoileal segment, and associated with thick, straight but regular folds. Mesenteric varices were suspected and led to angiographic studies which were normal in three cases, confirmed varices in eight cases, and thrombosis in four cases. Laparotomy was normal in three cases and established the etiological diagnosis in eight cases. Varices were shown in six cases. Arteriography and laparotomy were unable to reach a complete diagnosis. [less ▲]

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See detailL'âge est-il un facteur limitant pour la chirurgie carotidienne?
Van Damme, Hendrik ULg; Smitz, Simon ULg; Albert, Adelin ULg et al

in Journal des Maladies Vasculaires (1993), 18(3), 245-53

To evaluate the role of carotid surgery in elderly patients 75 years and older, we reviewed 912 carotid endarterectomies performed on 806 patients between 1987 and 1990. There were 151 patients (19% of ... [more ▼]

To evaluate the role of carotid surgery in elderly patients 75 years and older, we reviewed 912 carotid endarterectomies performed on 806 patients between 1987 and 1990. There were 151 patients (19% of the whole series) aged 75 years and older (160 endarterectomies, group A), including 29 octogenarians, vs 655 patients under 75 years of age (group B). There were more women in the elderly age group (44%) than in the younger one (38%). Symptoms, risk factors, operative outcome and follow up data of the two groups were compared. The risk profile was similar for the two age groups, with exception for coronary heart disease, less frequent in the older patient group (25% had previous infarction vs 44%). Indication for carotid endarterectomy was different in the two age groups: 41% of group A underwent prophylactic thrombendarterectomy for high degree stenosis, while only 30% of group B had asymptomatic carotid disease. In group A, 6% of the patients had carotid endarterectomy after recovering from a mild stroke, vs 2% in group B. Angiography revealed bilateral carotid disease in 59% of the group A patients (including 15% with contralateral occlusion) vs 40% in group B. Operative mortality was 1.5% for the younger age group vs 2.5% for the older age group. The cause of death was cardiac in 60%. A follow up is available for all patients who benefited carotid endarterectomy since 1976, including 180 patients aged 75 years or older.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailSynthetic model peptides for apolipoproteins. II. Characterization of the discoidal complexes generated between phospholipids and synthetic model peptides for apolipoproteins.
Corijn, J.; Deleys, R.; Labeur, C. et al

in Biochimica et Biophysica Acta (1993), 1170(1), 8-16

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied ... [more ▼]

The structure, composition and physico-chemical properties of complexes generated between phospholipids and synthetic model peptides for the amphipathic helices of the plasma apolipoproteins were studied. The sequences of the peptides were derived from that of the 18A peptide and designed to either enhance or decrease ionic interactions between pairs of peptides, as described in the accompanying paper. Complexes were prepared with dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), or with DPPC and cholesterol, and isolated on a Superose 6HR column. Association kinetics for the DMPC-peptides complexes were followed by measuring the turbidity as a function of the temperature. The diameters of the DPPC-peptide complexes, measured by gradient gel electrophoresis (GGE), were about 120 A. Fluorescence polarization measurements after labeling with diphenyl hexatriene (DPH) yielded transition temperatures of, respectively, 40.6, 41.5 and 41.8 degrees C for the DPPC/18AM1-, DPPC/18AM4- and DPPC/18A-peptide complexes. These values were confirmed by differential scanning calorimetry. Circular dichroism and infrared spectroscopy revealed that the peptides adopt an alpha-helical structure in solution and this percentage increased from 30-40% in the free peptides up to 50-60% in the complexes. Attenuated total reflection (ATR) infrared measurements of the complexes indicated that the peptides are oriented parallel to the acyl chains of the phospholipid bilayer. Denaturation of the peptides and of the peptide-lipid complexes was monitored by Trp fluorescence under addition of increasing amounts of GdmCl. The mid-points of the denaturation curves lie at, respectively, 0.05, 0.25 and 0.35 M GdmCl for the 18AM4, 18A and 18AM1 peptide and are shifted towards higher GdmCl concentrations after peptide-lipid binding. GdmCl denaturation decreased the alpha-helical content of the peptides and of the complexes, as monitored by circular dichroism measurement. The helix to random coil structure transition occurred at, respectively, 2.1, 2.2, and 2.0 M GdmCl for 18A, 18AM1 and 18AM4, compared to 5.1, 5.0, and 5.3 M in the corresponding complexes. These data suggest altogether that the structural properties, the mode of lipid-protein association and the stability of the phospholipid-peptide complexes are similar to those of native plasma apolipoproteins. The 18A and 18AM4 peptides which contain charged residues along the edge of the helix, leading to salt bridge formation between peptides were shown to mimic the amphipathic helices of the plasma apolipoproteins. [less ▲]

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See detailInventaire des végétaux éodévoniens de Belgique
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1993), 116

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See detailEnzymatic hydrolysis of reconstituted dimyristoylphosphatidylcholine-apo A-I complexes.
Lins, Laurence ULg; Piron, S.; Conrath, K. et al

in Biochimica et Biophysica Acta (1993), 1151(2), 137-42

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form ... [more ▼]

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form discoidal particles of defined dimensions. While the knowledge of the apo A-I sequence and secondary structure has been used to make predictions about its mode of association with lipids, the available experimental data necessary to propose a precise model of these discoidal structures are still limited. An important step in our understanding of these structures would be to identify the apolipoprotein lipid-associated domains. Proteolysis of apo A-I-DMPC reconstituted HDL (rHDL) and free apo A-I is used here to identify lipid-protected domains of apo A-I. Free cleaved peptides were separated from rHDL associated peptides by density gradient centrifugation. The lipid-associated peptides were further analyzed by SDS-PAGE and transferred by Western blot to a ProBlott membrane for sequencing. Cleavage occurred at residue 43 with proteinase K, 46 with trypsin and residue 47 or 48 with pronase. A large domain from about residue 45 to the C-terminal remains highly protected against hydrolysis eventhough it contains several bonds susceptible to proteolytic cleavage. No protected fragments were detected by SDS-PAGE after enzymatic cleavage of free apo A-I in identical experimental conditions. [less ▲]

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See detailSyngeneic Grafting of Adult Rat Drg-Derived Schwann Cells to the Injured Spinal Cord
Martin, Didier ULg; Schoenen, Jean ULg; Delree, P. et al

in Brain Research Bulletin (1993), 30(3-4), 507-14

A subdural inflatable micro-balloon was used to induce closed traumatic contusion to adult rat spinal cord. This spinal cord injury model was associated with reproducible and graded neurological deficits ... [more ▼]

A subdural inflatable micro-balloon was used to induce closed traumatic contusion to adult rat spinal cord. This spinal cord injury model was associated with reproducible and graded neurological deficits and histopathological alterations. At various delays after injury, transplantations of syngeneic adult cultured dorsal root ganglion-derived Schwann cells were performed into the spinal cord lesion. The transplants were well integrated and reduced the microcystic posttraumatic cavitation as well as the gliosis. Schwann cells transplants were invaded by numerous regenerating neurites most of which, based upon their neurotransmitter contents, seem to originate from the dorsal root ganglion. [less ▲]

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See detailThe fractal nature of the power spectrum as an indicator of chaos in the Bianchi-IX cosmological model
Demaret, Jacques; De Rop, Yves ULg

in Physics Letters B (1993), 299(3-4), 223-228

Using two different methods, we compute the fractal dimension of the power spectrum associated with numerical solutions of the spatially homogeneous Bianchi IX cosmological model in Misner-Chitre ... [more ▼]

Using two different methods, we compute the fractal dimension of the power spectrum associated with numerical solutions of the spatially homogeneous Bianchi IX cosmological model in Misner-Chitre coordinates. According to Perdang's criterion, the noninteger character of this dimension is reflection of chaos and can be used to measure it, in the same spirit as the Lyapunov exponent of the Kolmogorov entropy. The results obtained indicate the chaotic nature of the behaviour of the Bianchi IX model near the cosmological singularity. [less ▲]

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See detailNo significant effect of an "atraumatic" needle on incidence of post-lumbar puncture headache or traumatic tap.
Lenaerts, M.; Pepin, J. L.; Tombu, S. et al

in Cephalalgia : An International Journal of Headache (1993), 13(4), 296-7

We compared the incidence of post-lumbar puncture headache and of red blood cells in the first CSF sample (traumatic tap) in 35 inpatients punctured with a standard 20 G Yale needle and in 26 patients ... [more ▼]

We compared the incidence of post-lumbar puncture headache and of red blood cells in the first CSF sample (traumatic tap) in 35 inpatients punctured with a standard 20 G Yale needle and in 26 patients punctured with Sprotte's "atraumatic" needle. No significant difference was found between the two groups in incidence of headache or in frequency of traumatic taps. [less ▲]

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See detailProperties and primary structure of a cold-adapted lipase from an Antarctic bacterium
Arpigny, J. L.; Feller, Georges ULg; Gerday, Charles ULg

in Archives Internationales de Physiologie et de Biochimie (1993), 101

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See detailDynamics of erythropoietic recovery following bone marrow transplantation: role of marrow proliferative capacity and erythropoietin production in autologous versus allogeneic transplants.
Beguin, Yves ULg; Oris, Renée ULg; Fillet, Georges ULg

in Bone Marrow Transplantation (1993), 11(4), 285-92

The mechanisms of erythrocyte recovery after BMT are not known. We investigated the respective role of marrow function and erythropoietin production in 31 ABMT and 47 allogeneic BMT by analysing ... [more ▼]

The mechanisms of erythrocyte recovery after BMT are not known. We investigated the respective role of marrow function and erythropoietin production in 31 ABMT and 47 allogeneic BMT by analysing peripheral counts, serum erythropoietin levels, and serum transferrin receptor (TfR) levels which have been shown to be a quantitative measurement of erythropoiesis. Median times to complete neutrophil (25 vs 48 days, p < 0.0001) and platelet (45 vs 263 days, p < 0.001) recovery were faster after allogeneic BMT than ABMT, but complete erythrocyte recovery was slower (218 vs 101 days, p < 0.001). After ABMT, erythrocyte recovery paralleled that of neutrophils and platelets, and erythropoietin levels remained appropriate for the degree of anemia. After allogeneic BMT, erythrocytes developed independently of the other cell lines and defective erythropoietin production delayed recovery of adequate erythropoietic activity. This correlated with an alteration of renal function only in those patients remaining erythropoietin deficient beyond day 180. However, supranormal erythropoietin levels in interstitial pneumonia suggests that erythropoietin response to hypoxia is not abrogated. CMV infection could also affect erythropoiesis through erythropoietin production after ABMT as well as allogeneic BMT. It is concluded that after ABMT the development of erythropoiesis is determined by the overall marrow proliferative activity and erythropoietin plays only a facilitating role. After allogeneic BMT, erythropoiesis depends on erythropoietin levels which remain inadequate for prolonged periods of time. The results suggest that the administration of recombinant human erythropoietin could reduce transfusion requirements after BMT. [less ▲]

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See detailEnhancement of bovine leukemia virus-induced syncytia formation by di- and tripeptides.
Voneche, V.; Callebaut, I.; Lambrecht, B. et al

in Virology (1993), 192(1),

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by ... [more ▼]

Short hydrophobic peptides were previously shown to inhibit infectivity of para- and orthomyxoviruses. We tested the ability of a series of di- and tripeptides to interfere with cell fusion induced by bovine leukemia virus (BLV). Peptides containing a hydrophobic contribution and/or a positive net charge strongly enhanced syncytia formation induced by BLV on CC81 indicator cells. The size of the multinucleated cells was strongly increased (up to 10-fold) in the presence of the enhancer peptides whereas no effect was observed on the indicator cells in the absence of BLV. The peptides thus amplified the fusion process initiated by BLV envelope glycoproteins. The effect was dose-dependent at concentrations ranging from 20 to 640 microM and did not result from an increased expression of BLV proteins. The peptides did not compete with anti-gp51 monoclonal antibodies for the recognition of eight well-defined epitopes of gp51. We consequently hypothesize that the enhancer peptides interact with the membrane of BLV-producing cells and/or indicator cells and propose a model based on molecular modeling. [less ▲]

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See detailComparative study of the oxyhaemoglobin dissociation curve of four mammals: man, dog, horse and cattle
Clerbaux, Th; Gustin, Pascal ULg; Detry, B et al

in Comparative Biochemistry and Physiology. A : Comparative Physiology (1993), 106A(4), 687-694

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See detailSynthetic model peptides for apolipoproteins. I. Design and properties of synthetic model peptides for the amphipathic helices of the plasma apolipoproteins.
Brasseur, Robert ULg; Vanloo, B.; Deleys, R. et al

in Biochimica et Biophysica Acta (1993), 1170(1), 1-7

Amphipathic helical peptides are the lipid-binding motives of the plasma apolipoproteins, and synthetic peptide analogs have been used to unravel the mechanism of lipid association within this class of ... [more ▼]

Amphipathic helical peptides are the lipid-binding motives of the plasma apolipoproteins, and synthetic peptide analogs have been used to unravel the mechanism of lipid association within this class of proteins. Hydrophobic interactions between the apolar amino acid residues belonging to the hydrophobic face of the amphipathic helices and the lipids are the major driving forces in the peptide-lipid association to form discoidal complexes. Ionic interactions and salt bridge formation between contiguous peptide chains in the complex can, however, contribute to the overall stability of the lipid-protein particle. This was studied by designing peptide analogs to the helical repeats of the apolipoproteins with variable degrees of salt bridge formation between adjacent peptide chains. The most stable conformation for pairs of synthetic peptides was calculated by energy minimisation together with the energy of interaction between peptides. The sequence of the peptides was derived from that of the 18A peptide synthesized by Segrest et al., and the theoretical calculations confirmed that ionic interactions between residues close to each other, along the edge of two adjacent anti-parallel peptides, can significantly contribute towards the stability of a peptide-phospholipid complex. [less ▲]

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See detailOkadaic acid, a protein phosphatase inhibitor, enhances transcription of a receptor gene containing sequence A of the human prolactin promoter
Wera, S.; Belayew, A.; Martial, Joseph ULg

in Molecular Endocrinology (1993), 7(8), 965-71

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter ... [more ▼]

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter. We have investigated whether protein phosphatases could be involved in this regulation. GC-type rat pituitary tumor cells were transfected with sequence -138 to -35 of the hPRL gene promoter, upstream from a thymidine kinase promoter and a chloramphenicol acetyltransferase (CAT) reporter gene. Addition of okadaic acid (OA), a specific inhibitor of protein phosphatases 1 and 2A, stimulates transient expression of the CAT gene. The dose-response curve shows a maximal effect at 25 nM OA (2.2-fold stimulation above controls). The OA effect is also observed with a natural 4500-base pair hPRL promoter. A single copy of the hPRL promoter sequence -115 to -85 (sequence A) confers to a thymidine kinase-CAT construct an identical response to OA, whereas a single copy of the proximal Pit-1 binding site does not. Synergism is observed between cAMP and OA in activating PRL gene transcription. This synergism is also observed with a single copy of sequence A. The effect of cAMP is not mediated by an L-type Ca2+ channel, since addition of the Ca2+ channel antagonist verapamil does not decrease it, nor does complexing extracellular Ca2+ significantly reduce it. Furthermore, OA and the Ca2+ channel opener BAY K8644 exert additive effects. [less ▲]

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See detailFurther studies on the genetic diversity of lima beans (Phaseolus lunatus L.) using allozymes
Maquet, Alain; Wathelet, Bernard ULg; Baudoin, Jean-Pierre ULg

in Annual Report of the Bean Improvment Cooperative (1993), 36

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See detailLocalisation scintigraphique de tumeurs neuroendocrines et de leurs métastases par un analogue de la somatostatine: [111In-DTPA-d-phe1] octréotide.
Beckers, Albert ULg; Pham, V. T.; Abs, R. et al

in Revue Médicale de Liège (1993), 48(6), 326-34

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See detailLaminin Receptors and Laminin-Binding Proteins During Tumor Invasion and Metastasis
Castronovo, Vincenzo ULg

in Invasion & Metastasis (1993), 13(1), 1-30

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several controversies and confusion generated mainly by two events. First, the identification of several new laminin-binding proteins has raised the difficult task of attributing specific functions to specific receptors in contrast to initial beliefs that all cellular laminin-driven biological activities were mediated through the 67 LR. The second source of controversy is the large molecular-weight discrepancy between the 37-kD polypeptide encoded by the 67 LR cDNA clone and the mature 67 LR. In this manuscript, a critical and extensive review of the data accumulated on the 67 LR is presented regarding both its molecular structure and its role during tumor invasion and metastasis. A hypothetical model of the 67 LR is also proposed. Since the first identification of the 67 LR, at least 14 other cell surface molecules have been reported to be potential laminin receptors or laminin-binding proteins. These include members of the beta-galactoside-binding lectin family, seven members of the integrin family, the galactosyltransferase and some not yet fully characterized cell surface molecules. These laminin receptors and laminin-binding proteins are also described and their functions are also discussed with a particular emphasis on their participation in the constitution of the invasive phenotype. [less ▲]

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See detailFundic Argyrophil Cell Hyperplasia in Atrophic Gastritis: A Search for a Sensitive Diagnostic Method
Belaiche, Jacques ULg; Delwaide, Jean ULg; Vivario, M. et al

in Acta Gastro-Enterologica Belgica (1993), 56(1, Jan-Feb), 11-7

Hypergastrinemia induces argyrophil cell hyperplasia in oxyntic mucosa (FACH) in patients with non-antral atrophic gastritis, with or without pernicious anemia. This proliferation favours the development ... [more ▼]

Hypergastrinemia induces argyrophil cell hyperplasia in oxyntic mucosa (FACH) in patients with non-antral atrophic gastritis, with or without pernicious anemia. This proliferation favours the development of carcinoid tumours. In order to determine the most usual appropriate method to document FACH, we have studied 29 consecutive fundic biopsies from 26 patients with fundic chronic gastritis. The study encompassed gastrinemia levels, standard histology permitting the classification of chronic gastritis, demonstration of FACH by Grimelius stain, immunohistochemical studies using NSE, chromogranin A and by electron microscopy. The FACH was classified for each stain as slight, moderate or severe. The study displayed a relationship between the grade of gastritis and the density of argyrophil endocrine cells in oxyntic mucosa assessed by Grimelius stain (p < 0.0001) and chromogranin A (P < 0.01). There was also a relationship between the serum gastrin level and the density of argyrophil endocrine cells detected by these two stains (p < 0.001). A highly significant correlation was observed between Grimelius stain and chromogranin A (p < 0.0001). On the other hand, no significant correlation was noted with either NSE or electron microscopy. We conclude that Grimelius stain and immunohistochemical studies against chromogranin A were the best methods for the demonstration of FACH in atrophic gastritis. One of these two techniques is sufficient in current practice for defining the patients at risk for subsequent surveillance. [less ▲]

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See detailEffects on performance and meat characteristics of a diet supplemented with fat during the finishing period of growing fattening bulls
Hollo, Véronique; Marche, Christian; Dufrasne, Isabelle ULg et al

in Animal Production (1993), 56

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See detailMuscles rotateurs d'épaule et isocinétisme
Croisier, Jean-Louis ULg; Mommer, R.; Crielaard, Jean-Michel ULg

in Médecine et Hygiène (1993), 51

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See detailArt et nature
Duchesne, Jean-Patrick ULg

in Art&fact (1993), 12

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See detailExteroceptive suppression of temporalis muscle activity: methodological and physiological aspects.
Schoenen, Jean ULg

in Cephalalgia : An International Journal of Headache (1993), 13(1), 3-10

In recent years studies of the suppression of EMG activity in temporalis muscle induced by stimulation in the trigeminal territory have opened new perspectives in headache research. The various methods ... [more ▼]

In recent years studies of the suppression of EMG activity in temporalis muscle induced by stimulation in the trigeminal territory have opened new perspectives in headache research. The various methods that have been used in different laboratories are reviewed and some of the physiological modulations of temporalis exteroceptive suppression are described. Among different methods of recording, averaging 10 full-wave rectified EMG responses produces results with acceptable variability and discomfort. In order to obtain maximal responses the intensity of the stimulation should reach at least 20 mA. To avoid habituation of the second temporalis exteroceptive suppression period (ES2), the stimulation frequency has to be at 0.1 Hz or below. The level of voluntary contraction is not a critical variable as long as it reaches 50% of maximum. Some physiological variations of temporalis suppression are well documented. In females, ES2 is shorter during menstruation than at mid-cycle and correlated with the estradiol/progesterone ratio in plasma. Conditioning temporalis ES2 by a preceding peripheral stimulus markedly reduces its duration, which is partly reversible by naloxone. Various pharmacological agents are able to modify temporalis ES2: its duration is increased by 5-HT1 antagonists, but decreased by 5-HT uptake blockers; contradictory results have been obtained with acetylsalicylic acid. These results suggest that inhibitory brain-stem interneurons mediating temporalis ES2 are inhibited by serotonergic afferents, probably from the raphe magnus nucleus, and that the latter receives an excitatory input from the periaqueductal gray matter and other limbic structures, in part via opioid receptors. [less ▲]

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See detailDRC1 expression on normal and pathological lymphoid cells.
Antoine, Nadine ULg; Heinen, Ernst ULg; Marcoty, C. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailMachine Intelligent Automatic Recognition of Critical Mobile Targets in Laser Radar Imagery
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

in Lincoln Laboratory Journal (1993), 6(1), 161-186

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See detailSTUDY OF THE CRYSTALLIZATION PROCESS IN BI2-XPBXSR2CA2CU3O10-Y GLASS SYSTEMS - OPTICAL POLARIZED-LIGHT MICROSCOPY, ELECTRICAL AND MAGNETIC-PROPERTIES
Cloots, Rudi ULg; Romain, Anne-Claude ULg; Rulmont, André ULg et al

in Superconductor Science & Technology (1993), 6(12), 850-857

Synthesis of Bi2-xPbxSr2Ca2Cu3O10-y (x = 0 and x = 0.3) has been performed using the glass recrystallization route technique. After splat quenching, the samples were annealed at 820, 850 or 870-degrees-C ... [more ▼]

Synthesis of Bi2-xPbxSr2Ca2Cu3O10-y (x = 0 and x = 0.3) has been performed using the glass recrystallization route technique. After splat quenching, the samples were annealed at 820, 850 or 870-degrees-C for 96 or 144 h (with one intermediary grinding). High-resolution polarization micrographs and electrical (and magnetic susceptibility) measurements have been taken. Various growth mechanisms can thus be checked, and the physical properties are studied in relation to the microstructure. It is found that the 2223 phase does not crystallize directly from the amorphous one but results from a dissolution-reprecipitation process. The stability of the 2212 phase is emphasized. The 2201 phase precipitates at high temperature and rapidly reacts with the melt to form the 2223 phase. The kinetics of the lead-substituted sample seems to be controlled by the Ca2PbO4 phase. which acts as a flux. [less ▲]

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See detailThe Implications of European Union for the Performance of Public Enterprises
Gathon, Henry-Jean ULg; Pestieau, Pierre ULg

in Administration : Journal of the Institute of Public Administration of Ireland (1993), 41(Summer), 149-165

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See detailLe rôle central du thymus dans l'éducation des cellules T aux principes neuroendocrines
Geenen, Vincent ULg

in Verhandelingen - Koninklijke Vlaamse Academie voor Geneeskunde van Belgie (1993), LV

The thymus is the site of expression of peptides belonging to the neurohypophysial peptide family. The classical model of neurosecretion established by the Scharrers for the hypothalamo-neurohypophysial ... [more ▼]

The thymus is the site of expression of peptides belonging to the neurohypophysial peptide family. The classical model of neurosecretion established by the Scharrers for the hypothalamo-neurohypophysial axis howver cannot be applied to characterize the secretory pathways of neurohypophysial-related peptides in the thymic epithelium. The novel model of cryptocrine signaling has been proposed by John W. Funder to describe the molecular relationships between fixed epithelial cells and migratory differentiating cells. In the thymus, cryptocrine signaling is further closely associated to the presentation of the 'self' molecular structure by major histocompatibility complex-derived proteins to developing T cells. Altogether, the model of the thymic repertoire of neuroendocrine self-antigens transposes to the molecular level the dual physiological role of the thymus in T cell negative and positive selection. Moreover, this model should also contribute to a better understanding of the molecular mechanisms underlying the establishment of central T-cell self-tolerance to neurodocrine functions. [less ▲]

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See detailComparing measures of poverty and relative deprivation: an example for Belgium
Delhausse, Bernard; Luttgens, Axel; Perelman, Sergio ULg

in Journal of Population Economics (1993), 6

This paper presents three different poverty standards. A first approach takes the disposable income as an indicator of poverty. A second approach uses the Leyden approach. Finally an aggregate index of ... [more ▼]

This paper presents three different poverty standards. A first approach takes the disposable income as an indicator of poverty. A second approach uses the Leyden approach. Finally an aggregate index of deprivation, based on the observation of consumption events, is constructed through a particular econometric procedure proposed by Desai and Shah (1988). These alternative measures are then compared on a sample composed of 6380 Belgian households. Such an analysis can be expected to provide some further insight into the problem of measuring poverty, which has been the subject of a recent controversial debate. [less ▲]

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See detailLa morphologie synchronique du nom latin. Une bibliographie de base (1962-1991)
Pietquin, Paul ULg; Isebaert, Lambert

in Orbis : Bulletin international de documentation linguistique (1993), 36

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See detailPotato chloroplasts express an intron-encoded maturase-like polypeptide
du Jardin, Patrick ULg; Portetelle, Daniel ULg; Harvengt, L. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1993), 101

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See detailMonoamine Oxidase Inhibitors and Anesthesia: An Updated Literature Review
Blom-Peters, L.; Lamy, Maurice ULg

in Acta Anaesthesiologica Belgica (1993), 44(2), 57-60

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these ... [more ▼]

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these anesthetics. These drugs allow selective and rapidly reversible inhibition of type A-MAO. The pharmacologic effects of this new class of drug as well as their potential interactions with agents used in anesthesia are reviewed. [less ▲]

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See detailAtomic data and the spectrum of the solar photosphere.
Grevesse, Nicolas ULg; Noels-Grötsch, Arlette ULg

in Physica Scripta (1993), 47

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the ... [more ▼]

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the atomic data, especially the transition probabilities for faint lines. The authors justify this request for many more very accurate gf-values through a few particularly striking points. [less ▲]

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See detailFollicular dendritic cells: isolation procedures, short and long term cultures.
Heinen, Ernst ULg; Tsunoda, R.; Marcoty, C. et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailEffect of androgen treatmen on fear reactions in ewes
Vandenheede, Marc ULg; Bouissou, M. F.

in Hormones & Behavior (1993), 27

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See detailIsopyoverdin Pp BTP1, a biogenetically interesting novel siderophore from Pseudomonas putida
Jacques, Philippe; Gwose, Inga; Seinsche, Dieter et al

in Natural Product Letters (1993), 3

From the cultures of Pseudomonas putida BTP 1 a siderophore could be isolated which contains the chromophore 2a rather than 1a typical for pyoverdins. Its structure was elucidated by MS, NHR and chemical ... [more ▼]

From the cultures of Pseudomonas putida BTP 1 a siderophore could be isolated which contains the chromophore 2a rather than 1a typical for pyoverdins. Its structure was elucidated by MS, NHR and chemical degradation. This new structural type is of great interest. The currently accepted biogenetic pathway leading to 1a starts from D-Tyr and L-Dab which condense to give the ferribactin chromophore 4. Subsequent ring closure via the -nitrogen of Dab leads to 1a while alternative ring closure via the -nitrogen would yield 2a. It is for the first time that this iso-chromophore has been detected in a Pseudomonas siderophore. Its discovery confirms the assumed intermediacy of ferribactine in the biogenesis of pyoverdins. [less ▲]

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See detailEvidence that insulin-like growth factor 2 (IGF2) is the dominant thymic peptide of the insulin superfamily
Geenen, Vincent ULg; Achour, Imane; Robert, Françoise et al

in Thymus (1993), 21(2), 115-127

Central T-cell tolerance of neurondocrine functions has been proposed to be primarily induced by the thymic repertoire of neuroendocrine self antigens. The present study aimed at characterizing the human ... [more ▼]

Central T-cell tolerance of neurondocrine functions has been proposed to be primarily induced by the thymic repertoire of neuroendocrine self antigens. The present study aimed at characterizing the human thymic insulin-related self antigen able to represent the pancreatic islet ß cell function in face of the developing T cells. Immunofluorescence studies were performed on human and rat thymic sections, as wess as on the rat IT-45R1 thymic epithelial cell line using several antibodies to epitopes of the insulin peptide family. These studies identify beyond any doubt that IGF2 is the dominant thymic peptide of the insulin family. The sequence of an insulin-derived autoantigen is proposed. This autoantigen is a nonamer and has a hydrophobic residue leucine at position 9. In human species, this autoantigen would primarily be tolerogenic for the pancreatic ß-cell endocrine function during fetal development. [less ▲]

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See detailL'antiquité classique comme source d'inspiration dans l'oeuvre de Karel Tchapek
Mouton, Charles ULg

in Slavica Gandensia (1993), 20

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See detailVers une optimalisation de la formation langagière de futurs cadres: une expérience en semi-autonomie à la K.U.Leuven
Sanctobin, V.; Slootmaekers, A.; Vanden Berghe, Kristine ULg et al

in Association Belge de Linguistique Appliquée Papers (1993), 15

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See detailHormonal regulation of adult partner preference behavior in neonatally ATD-treated male rats.
Bakker, Julie ULg; Brand, T.; van Ophemert, J. et al

in Behavioral Neuroscience (1993), 107(3), 480-7

Male rats, neonatally treated with ATD (1,4,6-androstatriene-3,17-dione), which blocks the aromatization of testosterone into estradiol (E2), were tested for adult partner preference behavior (PPB ... [more ▼]

Male rats, neonatally treated with ATD (1,4,6-androstatriene-3,17-dione), which blocks the aromatization of testosterone into estradiol (E2), were tested for adult partner preference behavior (PPB; estrous female vs. active male). Castration caused a decrease in preference for the female partner in all males, with ATD males showing lower preference for the female partner than controls. Long-term castrated males did not show preference for either partner. Precastration levels of PPB in control males occurred after treatment with E2 or dihydrotestosterone (DHT) plus E2. DHT alone had no effect on PPB. With E2 alone, the ATD males clearly preferred the male partner. When DHT was added, these ATD males showed no preference for either partner or a low preference for the female partner. In conclusion, adult PPB in male rats is activated by endogenous testosterone or by both its metabolites (DHT and E2) or by E2 alone. ATD males showed a much lower preference for the female. There was a differential effect of DHT and E2: DHT had no effect, but E2 clearly caused ATD males to prefer the male partner and control males to prefer the female partner. [less ▲]

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See detailMicroencapsulation by Coacervation of Poly(lactide-co-glycolide)-II:Encapsulation of a Dispersed Aqueous Phase
Nihant, Nicole; Stassen, S; Grandfils, Christian ULg et al

in polymer International (1993), 32

This paper is related to the phase separation of different copolyesters of lactides and glycolide solutions induced by the addition of silicone oil in order to promote microencapsulation of proteins. This ... [more ▼]

This paper is related to the phase separation of different copolyesters of lactides and glycolide solutions induced by the addition of silicone oil in order to promote microencapsulation of proteins. This coating process can be divided in three successive steps: phase separation of the coating polymer; adsorption of the coacervate droplets around the drugs phase, and microcapsule solidification. This paper focuses on the physico-chemical analysis of the second step. The knowledge of the interfacial tensions between the three liquid phases allows understanding of the microscopic evolution of the phase separation medium. [less ▲]

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See detailA method of comparison of two close batches data: Application to analysis of fog formation causes
Boreux, Jean-Jacques ULg; Guiot, Joël

in Geophysical Research Letters (1993), 20(12), 1179-1182

Given suitable conditions of air temperature and humidity, the density of a fog and its microphysical properties depend mainly on the availability of cloud condensation nuclei (CCN) and their nature. Fogs ... [more ▼]

Given suitable conditions of air temperature and humidity, the density of a fog and its microphysical properties depend mainly on the availability of cloud condensation nuclei (CCN) and their nature. Fogs become particularly dense near certain industrial plants because of high concentration of hygroscopic combustion particles in the air. Their role in dense fog formation is estimated by comparing the local climates and CCN concentrations at two similar sites, the first being more subject to air pollution and dense fogs than the second. Orthogonal regression is applied to three meteorological variables (air temperature, relative humidity, wind speed) and CCN concentration. As we compare very close variables, bootstrap provides precise confidence intervals independent of Gaussian assumptions. Two sites are compared: they are located in the Meuse valley (Belgium) at a distance of about 15 km. We found that the local climate of the polluted site is not only colder and wetter but also richer in CCN that the control site. These results suggest interactions of natural and anthropogenic causes in dense fog formation at industrial site. This method is useful in various domains of geophysics when correlated time series have to be compared. [less ▲]

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See detailCloning and overexpression of the triosephosphate isomerase genes from psychrophilic and thermophilic bacteria. Structural comparison of the predicted protein sequences
Rentier-Delrue, Françoise ULg; Mande, Shekhar C; Moyens, Sylvianne et al

in Journal of Molecular Biology (1993), 229(1), 85-93

We focused on the temperature adaptation of triosephosphate isomerase (TIM; E.C. 5.3.1.1.) by comparing the structure of TIMs isolated from bacterial organisms living in either cold or hot environments ... [more ▼]

We focused on the temperature adaptation of triosephosphate isomerase (TIM; E.C. 5.3.1.1.) by comparing the structure of TIMs isolated from bacterial organisms living in either cold or hot environments. The TIM gene from psychrophilic bacteria Moraxella sp. TA137 was cloned and its nucleotide sequence determined. Its deduced amino acid sequence revealed 34% identity with the thermophilic bacteria Bacillus stearothermophilus TIM. Expression vectors were constructed and recombinant Moraxella TA137 and Bacillus stearothermophilus TIMs were overproduced and purified to homogeneity. Recombinant TIM inactivation constants (Ki), measured at various temperatures, compared to those of the mesophilic Escherichia coli recombinant TIM clearly show that Moraxella TA137 and B. stearothermophilus TIMs have respectively psychrophilic and thermophilic characteristics. To try to elucidate the structure-thermolability and structure-thermostability relationship, factors affecting the overall stability of these two TIMs were examined, based on the alignment with the mesophilic chicken TIM, the three-dimensional structure of which is already known. From this comparison, it appears that the adaptability of TIM to high temperature is favored by better stabilizing residues for the helix dipole as well as better helix-forming residues whereas the adaptability of TIM to low temperature seems to reside in the nature of helix-capping residues. [less ▲]

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See detailThe peripheral analgesic effects of opiods
Joris, Jean ULg; Hargreaves, Kenneth M

in APS Journal (1993), 2(1), 51-59

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See detailAspects cliniques de la néoplasie endocrinienne multiple de type 2 (NEM-2)
Moreau, L.; Beckers, Albert ULg

in Médecine & Chirurgie Digestives (1993), 23(1), 17-20

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See detailWanze/Huccorgne: Station de l'Hermitage
Noiret, Pierre ULg

in Chronique de l'Archéologie wallonne (1993), 1

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See detailMicroclimat et composition de l'air dans une enceinte fermée destinée à l'étude de la toxicité des polluants atmosphériques chez le porcelet
Urbain, B.; Gustin, Pascal ULg; Prouvost, J. F. et al

in Veterinary Research (1993), 24(6), 503-514

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate ... [more ▼]

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate, total dust, total viable particles, endotoxins in respirable dust (< 5 microns) and ammonia were measured in a 1.9 m3 environmental chamber designed for piglets. The activity of 1 piglet was recorded on video. Temperature and relative humidity were respectively 23.9 +/- 1.3 degrees C and 70.5 +/- 8.3% (mean +/- SD). Air flow rate was 10 m3/h. Dust, viable particles, endotoxin and ammonia concentrations were respectively 7 +/- 2 particles/ml, 3.4 x 10(4) +/- 2.9 x 10(4) BCFP (bacterial colony-forming particles)/m3, 4.8 +/- 1.5 ng/m3 and 6 +/- 0.8 ppm (mean +/- SD). All these parameters were comparable to the lowest values recorded in pigpens [less ▲]

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See detailCompte-rendu de l'ouvrage : Avignon au Moyen Age. Textes et documents
Xhayet, Geneviève ULg

in Moyen Age (Le) (1993), 99

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See detailLong-Term Photometry of Variables at ESO. II. The Second Data Catalogue (1986-1990).
Sterken, C.; Manfroid, Jean ULg; Anton, K. et al

in European Southern Observatory Scientific Report (1993), 12

Not Available

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See detailIdentification of eae genes in rabbit attaching and effacing enteropathogenic Escherichia coli (EPEC)
Pohl, P.; Peeters, J. E.; Jacquemin, E. R. et al

in Infection and Immunity (1993), 61

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See detailTYPE OF WEAR FOR THE PAIR TI6A14V/PCTFE IN AMBIENT AIR AND IN LIQUID-NITROGEN
Bozet, Jean-Luc ULg

in Wear (1993), 162(Part B), 1025-1028

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V ... [more ▼]

In the development of large, high pressure valves for cryotechnic rocket engines, the Tribology Department of the University of Liege had the opportunity to evaluate the pair Ti6Al4V/polychlorotrifluoroethylene (PCTFE) on a pin-on-disk tribometer in ambient air and liquid nitrogen with the contact pressure and sliding speed ranging, respectively, from 3 to 9 MPa and 0.03 to 0.05 m s-1. Because of the affinity of titanium for halogen elements, such as chlorine and fluorine, which are both present in PCTFE, we noticed the formation of an abrasive compound made of chlorine, fluorine, titanium and aluminium. These elements were detected by electron microscopy. In both environments the heat produced during sliding is responsible for the decomposition of the PCTFE. The surface of the polymer is softened by the heat and embedded abrasive particles then scratch the sliding track of the Ti6Al4V disk. This abrasiveness is confirmed with a three-dimensional stylus profilometer used on the Ti6Al4V disks. The low temperature and neutrality of liquid nitrogen hardly inhibit the decomposition of the polymer. This is related to the poor thermal conductivity of both titanium and PCTFE: the heat produced at the contact is fully available for the chemical reaction between the chlorine, fluorine and titanium, all the more so since this reaction requires little energy to take place. A better understanding of the wear behaviour of the pair PCTFE/Ti6Al4V in both air and liquid nitrogen should lead to the development of a surface treatment which prevents the formation of abrasive particles. We could then use titanium alloys as bulk materials for sliding parts in cryogenic valves and, because of the low specific mass of titanium, could benefit from the subsequent weight reduction. [less ▲]

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See detailOstéoporose postménopausique: le point sur la thérapeutique
Reginster, Jean-Yves ULg

in Medisearch (1993), 73

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See detailLes mécanismes biochimiques développés par les Pseudomonas fluorescents dans la lutte biologique contre les maladies des plantes transmises par le sol
Jacques, Philippe; Delfosse, Philippe; Ongena, MARC ULg et al

in Cahiers Agricultures (1993), 2

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See detailLa didactique universitaire : des méthodes et techniques à la redéfinition des objectifs
Merenne-Schoumaker, Bernadette ULg

in Education-Formation (1993), (232), 25-32

Courte réflexion sur la pédagogie universitaire portant à la fois sur les méthode set techniques et les objectifs de l'enseignement

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See detailEtude de l'effet de 2 additifs à base d'extrait de Yucca sur la réduction des émissions d'azote ammoniacal par des lisiers de porcs
Marlier, Didier ULg; Nicks, Baudouin ULg; Canart, Bernard

in Annales de Médecine Vétérinaire (1993), 137(7), 485-493

The effects of two commercial yucca extracts on the ammonia release from pig slurry have been tested in laboratory and in one fattening house. In laboratory, tests were carried on with 4 litre samples ... [more ▼]

The effects of two commercial yucca extracts on the ammonia release from pig slurry have been tested in laboratory and in one fattening house. In laboratory, tests were carried on with 4 litre samples whose ammonia production was recorded during 35 days. One of the two products, at the factory recommanded dose of 5 g/l, has successfully lowered the ammonia emission rate of the slurry. In the pig house, the two same products were given in the pigs' diet at the dose of 160 and 200 g/ton of feed. Inside a same building, pigs of one room received feed plus yucca extracts and those of the second room feed alone; none of the 2 products has significantly reduced the ammonia concentrations recorded on a three month period. One can expect improvements of the yucca extracts but up to now no guaranty can be given of their efficiency. [less ▲]

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See detailON THE FORMATION OF PHOTOSYSTEM-II CHLOROPHYLL PROTEINS AFTER A SHORT LIGHT FLASH IN ETIOLATED BARLEY LEAVES, AS MONITORED BY INVIVO FLUORESCENCE SPECTROSCOPY
Franck, Fabrice ULg

in Journal of Photochemistry and Photobiology B : Biology (1993), 18(1), 35-40

The biogenesis of photosystem II (PSII) was investigated using 77 K fluorescence spectroscopy in etiolated barley leaves subjected to a millisecond flash which reduced all protochlorophyllide (Pchlide ... [more ▼]

The biogenesis of photosystem II (PSII) was investigated using 77 K fluorescence spectroscopy in etiolated barley leaves subjected to a millisecond flash which reduced all protochlorophyllide (Pchlide) into chlorophyllide (Chlide). In darkness after the flash, a slow chlorophyll (Chl) band shift from 682 to 684 nm was associated with the development of PSII variable fluorescence. Two Chl fluorescence bands at 678 and 687 nm were formed during the shift, the 687 nm band corresponding to the spectrum of the PSII variable fluorescence. The development of PSII fluorescence was inhibited by chloramphenicol and enhanced by cyclobeximide, suggesting a competition between plastid- and nucleus-encoded proteins for Chl binding. [less ▲]

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See detailMathematical Programming Methods for Constrained Optimization: Dual Methods
Fleury, Claude ULg

in Progress in Astronautics and Aeronautics (1993), 150

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See detailThe Transcriptional Regulation of the Growth Hormone Gene Is Conserved in Vertebrate Evolution
Argenton, F.; Vianello, S.; Bernardini, S. et al

in Biochemical and Biophysical Research Communications (1993), 192(3), 1360-1366

Growth hormone (GH) gene expression in mammals is regulated by the interaction of the transcription factor Pit-1 with two binding sites within the proximal promoter. Four sequences, homologous to the ... [more ▼]

Growth hormone (GH) gene expression in mammals is regulated by the interaction of the transcription factor Pit-1 with two binding sites within the proximal promoter. Four sequences, homologous to the mammalian Pit-1 motif occur in the rainbow trout (Oncorhynchus mykiss) GHII (rtGH) gene promoter, two of which partly overlap. The three regions containing these putative Pit-1 binding sequences were protected from deoxyribonuclease I digestion by nuclear extracts of GC cells, a rat pituitary tumor cell line producing Pit-1. In gel shift assays, nuclear proteins from GC cells and from trout pituitaries were found to interact specifically with one of these protected sites. Transfection experiments showed that the rtGH promoter is transcnptionally active in GC cells, the response being strongly enhanced in the presence of a cAMP analogue. The results demonstrate that rat Pit-1 binds to and activates the rtGH promoter, indicating that the basic mechanisms regulating GH gene transcription have been conserved between fish and mammals. [less ▲]

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See detailOrganization of partner preference and sexual behavior and its nocturnal rhythmicity in male rats.
Bakker, Julie ULg; van Ophemert, J.; Slob, A. K.

in Behavioral Neuroscience (1993), 107(6), 1049-58

Male rats were neonatally treated with 1,4,6-androstatriene-3,17-dione (ATD), which blocks aromatization of testosterone (T) to estradiol (E2), from Days 0, 2, or 5 through 14. Adult partner preference ... [more ▼]

Male rats were neonatally treated with 1,4,6-androstatriene-3,17-dione (ATD), which blocks aromatization of testosterone (T) to estradiol (E2), from Days 0, 2, or 5 through 14. Adult partner preference behavior (PPB; choice between estrous female rat [F] and active male rat [M]) was studied in the early part of the dark phase of the light-dark (LD) cycle. ATD Day 0 Ms showed a preference for the stimulus M or showed no preference for either of the stimulus Ss. Controls preferred the estrous F. ATD Days 2 and 5 Ms showed PPB intermediate between ATD Day 0 Ms and controls. Thus the neonatally sensitive period for organization of adult PPB extends beyond Day 5. Furthermore, PPB showed a nocturnal rhythmicity in ATD Ms but not in controls. In the late part of the dark phase, all Ms showed a preference for the stimulus F. ATD Days 2 and 5 Ms and control Ms were no longer different in PPB, but ATD Day 0 Ms still showed significantly lower preference scores for F than all other Ms. Thus the E2 metabolite of T suppresses organization of an adult nocturnal rhythm in PPB. [less ▲]

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See detailLes formations frasniennes du Massif de Philippeville
Boulvain, Frédéric ULg; Coen, M.; Coen-Aubert, M. et al

in Professional Paper Belgian Geological Survey (1993), 259

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See detailProduction of hemicellulase by Penicillium canescens 10-10C. Arch. Int. Phys. Bioch., 101, B1-B00.
Fonder, C.; Rikir, R.; Kvesitadze, G. et al

in Archives Internationales de Physiologie et de Biochimie (1993), 101(B1-B100),

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See detailMiddle Devonian to Lower Carboniferous Miospores from selected boreholes in Amazonas and Parnaiba basins (Brazil): additional data, synthesis, and correlation.
Loboziak, S; Streel, Maurice ULg; Caputo, MV et al

in Documents du laboratoire de Géologie de Lyon (1993), 125

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