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See detailHeparin-coated Palmaz-Schatz stents in human coronary arteries. Early outcome of the Benestent-II Pilot Study.
Serruys, P. W.; Emanuelsson, H.; van der Giessen, W. et al

in Circulation (1996), 93(3), 412-22

BACKGROUND: The purpose of the Benestent-II Pilot Study was to evaluate the safety of delaying and eliminating anticoagulant therapy in patients receiving a heparin-coated stent in conjunction with ... [more ▼]

BACKGROUND: The purpose of the Benestent-II Pilot Study was to evaluate the safety of delaying and eliminating anticoagulant therapy in patients receiving a heparin-coated stent in conjunction with antiplatelet drugs. METHODS AND RESULTS: The study consisted of three initial phases (I, II, III) during which resumption of heparin therapy after sheath removal was progressively deferred by 6, 12, and 36 hours. In phase IV, coumadin and heparin were replaced by 250 mg ticlopidine and 100 mg aspirin. Of the 207 patients with stable angina pectoris and a de novo lesion in whom heparin-coated stent implantation was attempted, implantation was successful in 202 patients (98%). Stent thrombosis did not occur during all four phases, and the overall clinical success rate at discharge was 99%. Bleeding complications requiring blood transfusion or surgery fell from 7.9% in phase I to 5.9%, 4%, and 0% in the three following phases. Hospital stay was 7.4, 6.1, 7.2, and 3.1 days for the consecutive phases. The restenosis rate for the combined four phases was 13% (15% in phase I, 20% in phase II, 11% in phase III, and 6% in phase IV). The overall rate of reintervention for the four phases was 8.9%. At 6 months, 84%, 75%, 94%, and 92% of the patients of phases I to IV, respectively, were event free. For the four phases, the event-free rate was 86%, which compares favorably with the rate observed in the Benestent-I study (80%; relative risk, 0.68 [0.45 to 1.04]). CONCLUSIONS: The implantation of stents coated with polyamine and end-point-attached heparin in stable patients with one significant de novo coronary lesion is well tolerated, is associated with no (sub)acute stent thrombosis, and results in a favorable event-free survival after 6 months. [less ▲]

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See detailPrediction of response to recombinant human erythropoietin (rHuEpo) in anemia of malignancy.
Cazzola, M.; Ponchio, L.; Pedrotti, C. et al

in Haematologica (1996), 81(5), 434-41

BACKGROUND: Since only a portion of anemic patients outside the uremia setting benefit from erythropoietin treatment, a reliable means of predicting potential responders and nonresponders would be very ... [more ▼]

BACKGROUND: Since only a portion of anemic patients outside the uremia setting benefit from erythropoietin treatment, a reliable means of predicting potential responders and nonresponders would be very useful. MATERIALS AND METHODS: We retrospectively reviewed the clinical records of 58 patients with refractory anemia associated with various malignant disorders who had been treated with subcutaneous rHuEpo. The starting rHuEpo dose was 375 U/kg/week for 4 weeks, and was increased to 750 U/kg/week for another 4 weeks if no response was observed. Response was defined as a Hb increase > or = 2 g/dL with no need for blood transfusion. We examined the value of various laboratory parameters (baseline levels, 2-week and 4-week changes) as predictors of response. Endogenous erythropoietin production was evaluated by its serum level and erythroid activity was assessed through reticulocyte count and circulating transferrin receptor. RESULTS: Forty-eight individuals were evaluable, 58% of whom responded to rHuEpo within 8 weeks. Multiple regression analysis showed that 53% of the variation in the 8-week Hb concentration was explained by variations in baseline serum erythropoietin and the 2-week change in serum transferrin receptor (p < 0.001). Based on these two parameters, response prediction in individual patients would have resulted in a sensitivity of 96%, a specificity of 79% and an overall accuracy of 88%. In addition, 58% of the variation in the 8-week Hb was explained by variations in the 4-week changes in Hb and reticulocyte count (p < 0.001). Utilizing these latter parameters and baseline serum erythropoietin, response prediction in individual patients would have resulted in a sensitivity of 92%, a specificity of 82% and an overall accuracy of 88%. CONCLUSIONS: This retrospective analysis suggests that response to rHuEpo can be reasonably predicted by pretreatment serum erythropoietin together with early changes in simple laboratory parameters. [less ▲]

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See detailPremière approche de la flore macrolichénique corticole de la Région de Bruxelles-Capitale en situation de dépollution au SO2
Tanghe, Martin; Richel, Thierry; Crisan, Florin et al

in Belgian Journal of Botany (1996), 129

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See detailDélocalisation d'une division de l'entreprise et intérêt social
Thirion, Nicolas ULg

in Revue pratique des sociétés (1996), (1), 60-90

Les titulaires d'intérêts catégoriels dans une société (tels que les travailleurs) disposent-ils d'un droit d'agir en justice au nom de l'intérêt social, à la suite de la décision de délocalisation de l ... [more ▼]

Les titulaires d'intérêts catégoriels dans une société (tels que les travailleurs) disposent-ils d'un droit d'agir en justice au nom de l'intérêt social, à la suite de la décision de délocalisation de l'activité sociétaire? [less ▲]

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See detailActivités sportives et système immunitaire
PIRNAY, Freddy ULg; Bury, Thierry ULg

in Médecine et Hygiène (1996), 54

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See detailOn the structure of liquid tellurium
Bichara, C.; Raty, Jean-Yves ULg; Gaspard, Jean-Pierre ULg

in Journal of Non-Crystalline Solids (1996), 205-207

The atomic structure and the bonding mechanism in liquid tellurium are investigated by a tight binding Monte Carlo technique. The chain structure is preserved but a third covalent bond emerges with an ... [more ▼]

The atomic structure and the bonding mechanism in liquid tellurium are investigated by a tight binding Monte Carlo technique. The chain structure is preserved but a third covalent bond emerges with an intermediate interatomic separation (3.15 Aring). In addition a bond length alternation inside the chain appears in agreement with recent EXAFS experiments and the valence angles are significantly reduced. The electronic structure is studied and particular attention is paid to the modifications of lone pair interactions in the liquid structure [less ▲]

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See detailTribology in Aerospace Propulsion Mechanisms
Bozet, Jean-Luc ULg

in Space Scientific Research in Belgium, vol. IV : Space Technology (1996), 4

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See detailCongenital psoriasis following the lines of Blaschko.
Lehners-Weber, C.; de LA BRASSINNE, Michel ULg; Dezfoulian, Bita ULg et al

in Pediatric Dermatology (1996), 13(3), 219-221

Congenital psoriasis without an underlying nevus following the lines of Blaschko is described in a 6-year-old girl. In addition to this special type of psoriasis, she suffered from atopic dermatitis. The ... [more ▼]

Congenital psoriasis without an underlying nevus following the lines of Blaschko is described in a 6-year-old girl. In addition to this special type of psoriasis, she suffered from atopic dermatitis. The lesions were aggravated by sun exposure and phototherapy treatments, as well as by upper airways infections, without any clear explanation of these trigger factors. We propose to call this form of psoriasis congenital blaschkoid psoriasis. [less ▲]

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See detailImpairement of Memory for Spatial Context in Schizophrenia
Van der Linden, Martial ULg; Rohmer, J.G.; Rizzo, L. et al

in Neuropsychology (1996), 10

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See detailAnalysis of synchronization constraints and associated errors in collective work environments
Nyssen, Anne-Sophie ULg; Javaux, D.

in Ergonomics (1996), 39(10), 1249-1264

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See detailThe lichen genus Lecanactis and allied genera in Papua New Guinea
Egea, J.M.; Sérusiaux, Emmanuël ULg; Torrente, Pilar

in Mycotaxon (1996), 59

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See detailExpression of protein encoded by varicella-zoster virus open reading frame 63 in latently infected human ganglionic neurons
Mahalingam, Ravy; Wellish, Mary; Cohrs, Randall et al

in Proceedings of the National Academy of Sciences of the United States of America (1996), 93(5), 2122-2124

The ganglionic cell type in which varicellazoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open reading frame 63 protein, VZV open reading ... [more ▼]

The ganglionic cell type in which varicellazoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open reading frame 63 protein, VZV open reading frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system. [less ▲]

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See detailBenard-Marangoni instability in rigid rectangular containers
Dauby, Pierre ULg; Lebon, Georgy ULg

in Journal of Fluid Mechanics (1996), 329

Not Available

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See detailLa Grotte du Bois Laiterie, du Magdalénien au Mésolithique: différences comportementales
López Bayón, Ignacio; Straus, Lawrence; Otte, Marcel ULg et al

in Notae Praehistoricae (1996), 16

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See detailModulation Of Cd3-Gamma Gene Expression After Hiv Type 1 Infection Of The We17/10 T Cell Line Is Progressive And Occurs In Concert With Decreased Production Of Viral P24 Antigen
Willardgallo, Ke.; Delmellewibaut, C.; Segurazapata, I. et al

in Aids Research and Human Retroviruses (1996), 12(8),

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See detail"Born to be wild oder doch geleernt?" Neue verhaltensmedizinische Erkenntnisse zur Ätiopathogenese der Migräne
Gerber, W. D.; Kropp, P.; Schoenen, Jean ULg et al

in Verhaltenstherapie (1996), 6

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See detailFréquence et importance du charriage dans des rivières à charge de fond graveleuse
Petit, François ULg; Pauquet, A.; Pissart, Albert ULg

in Géomorphologie : Relief, Processus, Environnement (1996)

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See detailCombined Liver - Spleen -small intestine grafting in a rat model: Role of transplanting additional lymphoid tissue on survival.
D'Silva, Milbhor; Pirenne, Jacques; Nakhleh, R. E. et al

in International Surgery (1996), (81), 109-114

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See detailA field observation on the influence of aggressive behaviour on mating success in the European grayling
Poncin, Pascal ULg

in Journal of Fish Biology (1996), 48(4), 802-804

The biggest male grayling present on a spawning area was strongly territorial and developed more aggressive behaviours than smaller males. However, its spawning frequency was similar to that of smaller ... [more ▼]

The biggest male grayling present on a spawning area was strongly territorial and developed more aggressive behaviours than smaller males. However, its spawning frequency was similar to that of smaller males. (C) 1996 The Fisheries Society of the British Isles [less ▲]

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See detailChanges in nitrogen source modify distribution of excitation energy in the cyanobacterium Phormidium laminosum
deAlda, JAGO; Tapia, M. I.; Franck, Fabrice ULg et al

in Physiologia Plantarum (1996), 97(1), 69-78

In an attempt to clarify the interactions between the available nitrogen source and the photosystems in cyanobacteria, O-2 exchange and fluorescence emission were monitored in spheroplasts and intact ... [more ▼]

In an attempt to clarify the interactions between the available nitrogen source and the photosystems in cyanobacteria, O-2 exchange and fluorescence emission were monitored in spheroplasts and intact cells of the non N-2-fixing cyanobacterium Phormidium laminosum (strain OH-1-p.Cl-1) growing on different nitrogen sources or in the absence of nitrogen. Short-term (time scale of seconds to minutes), NH4+ addition to NO3--growing or N-starved cells and, to a minor extent, NO3- addition to N-starved cells, induced state 2 transitions both in light and dark. Long term (time scale of days), the fluorescence yield of PSI relative to that of PSII at 77 K was higher in NO3-- than in NH4-+ growing cells, and even higher in N-starved cells. In the dark, the plastoquinone pool was more reduced in NH4-+ than in NO3--growing cells. Both PSII and PSI activities and the degree of linking between both photosystems were affected in the long term, so that non-cyclic electron transport decreased in parallel to the ferredoxin requirement to assimilate each nitrogen source. Results indicate that nitrogen metabolism exerts short- and long-term control over the photosynthetic apparatus, which acclimates to the energy requirement of the available nitrogen source. [less ▲]

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See detailStimulus range effects in temporal bisection by humans.
Wearden, John H; Ferrara, André ULg

in Quarterly Journal of Experimental Psychology. B, Comparative & Physiological Psychology (1996), 49(1), 24-44

Two experiments with human subjects, using short-duration tones as stimuli to be judged, investigated the effect of the range of the stimulus set on temporal bisection performance. In Experiment 1, six ... [more ▼]

Two experiments with human subjects, using short-duration tones as stimuli to be judged, investigated the effect of the range of the stimulus set on temporal bisection performance. In Experiment 1, six groups of subjects were tested on a temporal bisection task, where each stimulus had to be classified as "short" or "long". For three groups, the difference between the longest (L) and shortest (S) durations in the to-be-bisected stimulus set was kept constant at 400 msec, and the L/S ratio was varied over values of 5:1 and 2:1. For three other groups, the L/S ratio was kept constant at 4:1 but the L-S difference varied from 300 to 600 msec. The bisection point (the stimulus value resulting in 50% "long" responses) was located closer to the arithmetic mean of L and S than the geometric mean for all groups except that for which the L/S ratio was 2:1, in which case geometric mean bisection was found. In Experiment 2, stimuli were spaced between L and S either linearly or logarithmically, and the L/S ratio took values of either 2:1 or 19:1. Geometric mean bisection was found in both cases when the L/S ratio was 2:1, but effects of stimulus spacing were found only when the L/S ratio was 19:1. Overall, the results supported a previous conjecture that the L/S ratio used in a bisection task played a critical role in determining the behaviour obtained. A theoretical model of bisection advanced by Wearden (1991) dealt appropriately with bisection point shifts discussed above but encountered difficulties with stimulus spacing effects. [less ▲]

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See detailRecently advanced computerized technology was applied to the investigation of morphometric, immunohistological and three-dimensional changes of the endometrial mucosa in order to evaluate quantitatively the effects of three doses of a new slow-release vaginal progesterone on the endometrium in post-menopausal women. A total of 20 menopausal women, deprived of ovarian function, were given oestrogen for 12 days and a combined therapy of oestrogen (administered orally) and progesterone for another 12 day period. Progesterone was administered vaginally through a new gel (Crinone) utilizing a bioadhesive, biocompatible polymer as a base to achieve a sustained release effect. An endometrial biopsy was taken before treatment, after oestrogen-only treatment and after the oestro-progestogen therapy. Before treatment, all the patients exhibited an atrophic endometrium. After oestrogen-only treatment, typical proliferative changes occurred: an increase in the endometrium thickness, an increase in the mitotic index, numerous cylinder-like glands and no coiled glands, and high concentrations of oestrogen receptors (ER) and progesterone receptors (PR). After the oestro-progestogen therapy, whatever the dose of progesterone given, a secretory transformation of the endometrial mucosa occurred, mitotic activity decreased significantly, more ramified and coiled glands were observed, and a decrease in PR content was noted in epithelial and stromal nuclei, and a decrease in PR content was also observed in epithelial nuclei but not in stromal nuclei. Accurate new techniques of image analysis have shown that crinone therapy could eliminate the proliferative effects of oestrogen treatment in post-menopausal women, despite doses as low as 45 mg of progesterone administered vaginally every other day. The results suggest that the sustained release effects of Crinone are clinically relevant.
Donnez, Jacques; NISOLLE, Michelle ULg; Casanas-Roux, Françoise et al

in Human Reproduction (1996), 11(1), 224-8

A computerized morphometrical investigation was performed on endometriotic tissue from the peritoneum (n = 225) and rectovaginal nodules (n = 65) to compare histologically and stereologically the ... [more ▼]

A computerized morphometrical investigation was performed on endometriotic tissue from the peritoneum (n = 225) and rectovaginal nodules (n = 65) to compare histologically and stereologically the rectovaginal septum endometriotic nodule to peritoneal endometriosis. Mitotic activity, stromal vascularization and the epithelium/stroma ratio were found to be significantly different in peritoneal and rectovaginal endometriosis. The evaluation revealed a major role of glandular epithelium in rectovaginal nodules where the stroma sometimes appeared absent around glandular epithelium. The study demonstrated opposite effects of gonadotrophin-releasing hormone agonists (GnRHa) and lynestrenol on the two lesions. Indeed, in peritoneal endometriosis, after GnRHa therapy, our study demonstrated a lower rate of mitosis and poor stromal vascularization. The same drug was unable to induce the same effects in the nodule although, in contrast, lynestrenol has a strong effect on nodule vascularization. In conclusion, it is suggested that the rectovaginal adenomyotic nodule is a specific disease, different from peritoneal endometriosis. It is not the consequence of 'deep infiltrating' endometriosis but can probably develop from Mullerian rests present in the rectovaginal septum. [less ▲]

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See detailNew isozymes markers for the study of the gene pool Phaseolus lunatus L.
Zoro Bi, Irié; Maquet, Alain; Wathelet, Bernard ULg et al

in Annual Report of the Bean Improvment Cooperative (1996), 39

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See detailBactericidal activity against Pseudomonas aeruginosa is acquired by cultured human monocyte-derived macrophages after uptake of myeloperoxidase.
Mathy-Hartert, M.; Deby, Ginette ULg; Melin, Pierrette ULg et al

in Experientia (1996), 52(2), 167-74

Myeloperoxidase (MPO) is an enzyme located within polymorphonuclear neutrophils capable of producing cytotoxic oxidant species that are particularly active against bacteria with polysaccharide capsules ... [more ▼]

Myeloperoxidase (MPO) is an enzyme located within polymorphonuclear neutrophils capable of producing cytotoxic oxidant species that are particularly active against bacteria with polysaccharide capsules. Pseudomonas aeruginosa (10(6) bacteria per 1ml) are killed within 1 h in vitro by a MPO/H2O2/C1- system (48mU=132ng of MPO). The question arose as to whether human macrophages would acquire cytotoxic activity when loaded with this enzyme. Monocytes were therefore isolated from human blood and cultured for up to ten days to induce maturation to macrophages. These cells lost endogenous MPO within five days while H2O2 production in response to stimulation by phorbol myristate acetate (10(-6)M) decreased to 23% within ten days. On the other hand, their capacity to take up exogenous MPO increased fourfold from day three to day ten. Human macrophages cultured from eight days (when both H2O2 production and MPO uptake were sufficient) were therefore used to study the effects of MPO uptake on cytocidal activity against Pseudomonas aeruginosa. After a 1 h MPO loading period, macrophages (5X10(5) cells per ml) were incubated in the presence of bacteria (0.5 to 2X10(6) bacteria per ml) for 2 h at 37 degrees C. At a bacteria/macrophage ratio of 1, only 34.8+/-7.0% of bacteria survived (compared to killing by non-loaded macrophages), while 74.4+/-9.3% survived at a ratio of 4. From these results, we conclude that loading macrophages with exogenous MPO could enhance their microbicidal activity, suggesting a potentially useful therapeutic application. [less ▲]

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See detailNéphropathie tubulo-interstitielle aigue avec uvéite: à propos d'un cas
Weber, T.; Beckers, C.; Kaye, O. et al

in Acta Clinica Belgica (1996), 51(1), 53-56

We report another case of acute interstitial nephritis with uveitis (TINU syndrome) in a 35-year-old woman. About thirty cases were described since the first ones 20 years ago. We discuss the assessment ... [more ▼]

We report another case of acute interstitial nephritis with uveitis (TINU syndrome) in a 35-year-old woman. About thirty cases were described since the first ones 20 years ago. We discuss the assessment needed to reach the diagnosis. The evolution is unusually favourable with steroid therapy. [less ▲]

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See detailSuccessful Tricuspid Valve Replacement and Pulmonary Valvulotomy for Carcinoid Heart Disease
DEFRAIGNE, Jean ULg; Jerusalem, O.; Soyeur, Daniel ULg et al

in Acta Chirurgica Belgica (1996), 96(4), 170-6

A case of successful tricuspid valve replacement with a mechanical prosthesis and pulmonary valvulotomy for carcinoid heart disease is reported. The patient was a 61 years old women. The primary tumor was ... [more ▼]

A case of successful tricuspid valve replacement with a mechanical prosthesis and pulmonary valvulotomy for carcinoid heart disease is reported. The patient was a 61 years old women. The primary tumor was in the terminal ileum. Liver metastasis and carcinoid syndrome were present since 8 years. After cardiac surgery, the patient survived 38 months and late death was related to disseminated metastasis. Even in case of metastasis, carcinoid tumor is slow growing. Without cardiac operation for correction of valvular lesions, terminal symptoms and death may often be related to cardiac operation for correction of valvular lesions, terminal symptoms and death may often be related to cardiac failure rather than to tumoral growth. Thus, even in presence of metastasis, cardiac surgery may be mandatory to improve both quality of life and survival. [less ▲]

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See detailLa Biologie clinique en pédiatrie: hypotonie et retard moteur
Misson, Jean-Paul ULg; Leroy, Patricia; Dubru, Jean-Marie ULg et al

in Acta Paediatrica Belgica (1996), 28(2), 95

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See detailComparative evaluation of impulse oscillometry and a monofrequency forced oscillation technique in clinically healthy calves undergoing bronchochallenges
Reinhold, Petra; MacLeod, D.; Lekeux, Pierre ULg

in Research in Veterinary Science (1996), 61

Multifrequency impulse oscillometry (IOS) was compared with a monofrequency forced oscillation technique (MFO) in calves undergoing experimentally induced bronchoconstriction and subsequent ... [more ▼]

Multifrequency impulse oscillometry (IOS) was compared with a monofrequency forced oscillation technique (MFO) in calves undergoing experimentally induced bronchoconstriction and subsequent bronchodilatation. The dynamic lung compliance (Cdyn) was also measured by conventional methods. For each test, the baseline mean and the responses to saline, a bronchoconstrictive agent (carbachol) and a bronchodilator (fenoterolhydrobromide) were calculated. Using the IOS, the information was markedly frequency-dependent. The resistance (R) and the magnitude of respiratory impedance (Z) were only sensitive at 5 Hz, leading to negative frequency dependence of these parameters as an indicator of peripheral airway obstruction. A high sensitivity for reactance (X) and phase angle phi values was observed between 5 and 20 Hz. For MFO (10 Hz), the parameters Ros (which includes resistive and capacitive components of the respiratory system), phase shift (psi), and the oscillatory derived compliance of the respiratory system (Crs) were of the greatest clinical potential. Crs showed a significant coefficient of linear correlation (r = 0.88, P < 0.001) with Cdyn. At the 10 Hz test frequency similar results were observed with MFO and IOS, suggesting that for healthy calves the measurement effect of an impulse is not significant. With respect to peripheral airway calibre, a test frequency less than 10 Hz appeared to be most sensitive and least variable. [less ▲]

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See detailCellular and molecular mechanisms involved in thymic T-cell education to neuroendocrine self principles
Geenen, Vincent ULg; Benhida, Abdellah; Kecha, Ouafae et al

in International Journal of Thymology (1996), 4

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See detailRemarques sur le bilinguisme gréco-latin
Rochette, Bruno ULg

in Etudes Classiques (Namur, Belgium) (1996), 64

Réflexions sur la coexistence de l'emploi des langues grecque et latine dans le monde méditerranéen antique, ce phénomène étant considéré principalement sous son aspect littéraire, dans une perspective ... [more ▼]

Réflexions sur la coexistence de l'emploi des langues grecque et latine dans le monde méditerranéen antique, ce phénomène étant considéré principalement sous son aspect littéraire, dans une perspective diachronique. Les pionniers de la littérature latine furent des affranchis de langue grecque qui s'exprimaient en latin. Dès la fin du deuxième siècle av. J.-C., même si l'apport des affranchis grecs à l'histoire romaine reste important, de nombreux Romains cultivés s'expriment en grec. Devant les ressources qu'offre la langue grecque, plus d'un auteur latin, jusqu'à la fin de la République, déplore la « patrii sermonis egestas ». Mais Cicéron affirme la richesse du lexique latin et entreprend avec un grand souci d'exactitude des traductions du grec en latin. Finalement, durant toute la période impériale, les deux langues se complètent et s'équilibrent. [less ▲]

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See detailLe vétérinaire et la qualité microbiologique des denrées alimentaires d’origine animale
Vindevogel, Henri ULg; Daube, Georges ULg; Denys, Jacques et al

in Annales de Médecine Vétérinaire (1996), 141

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See detailRéactions physiologiques face à des conditions extrêmes de chaleur et d'humidité relative: Etude des effets de l'exercice
Art, Tatiana ULg; Lekeux, Pierre ULg

in Pratique Vétérinaire Equine (1996), 28

The purpose of this study was to investigate the effect of a hot and humid environment on the pattern of breathing and the gas exchange in heavily exercising horses. Five healthy fit standardbred horses ... [more ▼]

The purpose of this study was to investigate the effect of a hot and humid environment on the pattern of breathing and the gas exchange in heavily exercising horses. Five healthy fit standardbred horses (493± 14 kg; 4± 0,5yrs) were investigated twice at eight days interval, once in temperated atmos¬pheric conditions (TC) (ambient temperature : 15o C; relative humidity : 55 %) and once in hot and humid conditions (HHC) (ambient temperature : 30°C; relative humidity : 75 %). The standardized treadmill exercise test consisted in 8 min warm-up and in 8 min exer¬cise (1 min at 1,7, 4, 7, 8, 9 and 10 m/sec and 2 min at 4 m/sec with a 6% slope). Respiratory airflow, tidal volume, respiratory frequency as well as the 02 and C02 fraction in the inspired and expired gases were continuously recorded, using 2 ultrasonic pneumo¬tachographs connected to a face mask and a mass spectrometer. Oxygen uptake (VO2, carbon dioxide output (VCO2,), respiratory quotient (RQ) and expired minute volume (VE.) as well as alveolar partial pressure in C02 (PAC02) were obtained on a breath-by--breath basis. The maximal values obtained during the highest intensity were used for the comparison between both tests. Heart rate and skip temperature were continuously recorded, using a heart rate recording system and a real time infrared thermographic unit, respecti¬vely. Venous blood was sampled before and 2 min after the completion of the test for hematological and biochemical analysis, namely plasma lactate (LA), beta-endorphins, cortisol (Cor). Running in HHC induced a significant decrease in peak VE, VO2, VCO2 and oxygen pulse, while RQ remained unchanged and PAC02 increased. The changes in skin temperature (ST), i.e. the difference between ST at rest and at 10 m/sec, was dramatically higher in HHC. As well, the test-induced changes, i.e. the differences between the values before and after the test, in Cor, beta-endorphins and LA values after the test were significantly higher. This study shows that performing a strenuous exercise in hot, humid conditions in unacclimatized horses induced a reduction of the aerobic capacity, i.e. decrease of the VO2 and increase of LA production. A relative hypoventilation could be partly responsible for this observation, but all the steps of the oxygen transport from the pulmonary ventilation to the muscle metabolism could also potentially be impaired in such conditions [less ▲]

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See detailCodon adjustment to maximise heterologous gene expression in Streptomyces lividans can lead to decreased mRNA stability and protein yield.
Lammertyn, E; Van Mellaert, L; Bijnens, A P et al

in Molecular & General Genetics [=MGG] (1996), 250(2), 223-9

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the ... [more ▼]

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the mTNF gene were therefore adapted to the codon bias of Streptomyces by site-directed mutagenesis. No improvement in mTNF yield could be detected. The stability of the transcript derived from the construct was shown to be more important for determining the final level of mTNF production. A strong correlation was observed between the yield of secreted biologically active mTNF and the amount of mTNF mRNA present in the cells. [less ▲]

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See detailEvaluation du débit sanguin au niveau du quadriceps électrostimulé et contracté volontairement
Vanderthommen, Marc ULg; BAUVIR, Philippe ULg; Depresseux, J.C. et al

in Annales de Kinésithérapie (1996), 23(1), 20-23

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See detailEvaluation of insulin-like growth factor-I and its plasma binding proteins in pigs.
Renaville, Robert ULg; Prandi, A.; Claes, Victor et al

in Archivio Veterinario Italiano (1996), 46

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See detailSequence stratigraphy of the Frasnian-Famennian transitional strata: a comparison between South China and Southern Belgium
Muchez, P.; Boulvain, Frédéric ULg; Dreesen, R. et al

in Palaeogeography, Palaeoclimatology, Palaeoecology (1996), 123

The sedimentological evolution of the Frasnian-Famennian transitional strata in South China and southern Belgium has been investigated. A similar trend in the deepening and shallowing of the sedimentation ... [more ▼]

The sedimentological evolution of the Frasnian-Famennian transitional strata in South China and southern Belgium has been investigated. A similar trend in the deepening and shallowing of the sedimentation environment occurs in the two palaeogeographically distinct areas. The stratigraphic succession has been subdivided into depositional sequences. A general deepening occurs in the Palmatolepis rhenana conodont zone. During the most rapid rise in sea level, a transgressive systems tract formed. This transgressive systems tract is followed by a highstand systems tract in the late P. rhenana zone. A major sequence boundary within the latter zone is indicated by an unconformity in intraplatform and platform areas and by a conformity in the basinal area. The uppermost sediments within the late rhenana zone correspond to a lowstand systems tract. A rapid flooding took place near the base of the Palmatolepis linguiformis zone and dark shales and limestones formed. A second sequence boundary coincides with or is very close to the Frasnian-Famennian boundary (base P. triangularis zone). The global extinction event at the Frasnian- Famennian boundary coincides with an important eustatic fall in sea level. The proposed stratigraphic subdivision should allow worldwide correlations of shallow and deep water deposits. [less ▲]

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See detailDifferential effects of gonadotropin-releasing hormone, dopamine and somatostatin and their second messengers on the mRNA levels of gonadotropin IIb subunit and growth hormone in the teleost fish, tilapia
Melamed, P.; Gur, G.; Elizur, A. et al

in Neuroendocrinology (1996)

In cultured pituitary cells of tilapia, gonadotropin-releasing hormone (GnRH; 10 nM 4-24 h), elevation of cyclic AMP (by 10 microM forskolin or 0.2 mM 3-isobutyl-1-methylxanthine: IBMX 0.5-36 h) or ... [more ▼]

In cultured pituitary cells of tilapia, gonadotropin-releasing hormone (GnRH; 10 nM 4-24 h), elevation of cyclic AMP (by 10 microM forskolin or 0.2 mM 3-isobutyl-1-methylxanthine: IBMX 0.5-36 h) or activation of protein kinase C (PKC; by 12.5 nM tetradecanoyl phorbol-13-acetate: TPA, 0.5-24 h) all increased gonadotropin (GtH) II beta steady state mRNA levels by three to four-fold. The involvement of PKA and PKC in the GnRH stimulatory effect on both GtH release and GtH II beta mRNA levels was corroborated by use of the PKA and PKC inhibitors, H89 and GF109203X, respectively (100 nM) which attenuated the GnRH effect. Incubation with actinomycin D (8 microM, 4-21 h) after preexposure for 24 h to either forskolin (10 microM) or TPA (12.5 nM), revealed that rates of transcript degradation were slower in forskolin-treated cells (T 1/2 = 14.1 h) than in control or TPA-treated cells (T 1/2 = 8.47 or 8.38 h), suggesting a stabilizing effect on the mRNA. Dopamine (DA; 10 microM, 4-36 h) had no apparent effect on steady state mRNA levels of GtH II beta, but reduced GtH release by as much as 75%. Steady state levels of growth hormone (GH) mRNA were not affected by exposure to GnRH (10 nM, 4-24 h), although GH release was more than doubled. Similarly, activation of PKC (by TPA 12.5 nM, 1.5-36 h), which was shown to be essential for the GnRH-stimulatory effect on GH release, did not alter levels of the GH transcript, but increased GH release by more than fivefold. DA (10 microM, 4-24 h) moderately increased GH transcript levels (160%) with similar kinetics but lower potency than direct elevation of cAMP (by 10 microM forskolin or 0.2 mM IBMX, 0.5-36 h) which increased transcript levels by more than fourfold. The involvement of PKA in the DA effect was confirmed when the PKA inhibitor H89 (100 nM, 15 min prior to DA exposure) attenuated the DA effect on GH mRNA levels. Exposure of cells to actinomycin D (8 microM, 2-16 h) after treatment with forskolin (10 microM, 24 h) led to a slower rate of transcript degradation than in control cells (T 1/2 = 6.5 h vs. T 1/2 = 4.36 h), suggesting that cAMP also elicits a stabilizing effect on GH mRNA. Somatostatin (100 nM, 0.5-36 h) had no clear effect on GH transcript levels, but reduced GH release by as much as 90%. These results suggest that activation of either cAMP-PKA or PKC pathways can, possibly by different mechanisms, stimulate mRNA levels of the GtH II beta gene, but that only the cAMP-PKA pathway stimulates GH mRNA levels. It would appear therefore that GnRH, although stimulating GH release, does not regulate GH transcription in this fish. [less ▲]

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See detailShedding of the 67-kD laminin receptor by human cancer cells.
Karpatova, M.; Tagliabue, E.; Castronovo, Vincenzo ULg et al

in Journal of Cellular Biochemistry (1996), 60(2), 226-34

The 67-kD laminin receptor (67LR) is a cell membrane-associated molecule exhibiting high affinity for the basement membrane glycoprotein, laminin. While export of the 67LR toward the extracellular matrix ... [more ▼]

The 67-kD laminin receptor (67LR) is a cell membrane-associated molecule exhibiting high affinity for the basement membrane glycoprotein, laminin. While export of the 67LR toward the extracellular matrix has been recently suggested by electron microscopy studies, there is to date no evidence of shedding of the 67LR from cells. Using two monoclonal antibodies directed against the 67LR, we developed a double-determinant radioimmunoassay that demonstrates that the 67LR is released from cancer cells into the culture medium. The shed molecule exhibited the same apparent molecular weight as that of the membrane-associated 67LR, suggesting that no proteolytic cleavage is involved in the process. Furthermore, we demonstrate that the 67LR is not anchored to the membrane through a glycolsyl-phosphatidylinositol bridge. However, the observation that lactose increased the release of 67LR suggests that a lectin-type interaction is involved in the cell membrane association of this laminin binding protein and the cell surface. Interestingly, the released 67LR recovered after HPLC gel filtration was found free as well as associated to high molecular weight complexes. The free 67LR retained its ability to bind to the cell surface. Our study is the first demonstration that the 67LR is effectively shed by cancer cells. The released free 67LR could play an important role in modulating interactions between cancer cells and laminin during tumor invasion and metastasis. [less ▲]

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See detailMise au point relative à l'utilisation de la gonadolibérine en reproduction bovine. 2. Domaines d'application clinique.
Hanzen, Christian ULg; Houtain, Jean Yves; Laurent, Yves

in Médecin Vétérinaire du Québec (1996), 26(2), 47-54

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See detailOrdovician and Silurian cryptospores and miospores from southeastern Turkey
Steemans, Philippe ULg; Le Hérissé, A; Bozdogan, N

in Review of Palaeobotany and Palynology (1996), 93(1-4), 35-76

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See detailAnatomie des monticules micritiques du Frasnien belge et contexte eustatique
Boulvain, Frédéric ULg; Herbosch, A.

in Bulletin de la Société Géologique de France (1996), 167(3), 391-398

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See detailImprovement in the inflammatory aspect of androgenetic alopecia. A pilot study with an antimicrobial lotion
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Tassoudji, Nazli ULg et al

in Journal of Dermatological Treatment (1996), 7

Androgenetic alopecia (AGA) is a common yet poorly understood condition. In particular, the significance of inflammatory cells close to the infrainfundibulum of transitional hairs remains obscure. This ... [more ▼]

Androgenetic alopecia (AGA) is a common yet poorly understood condition. In particular, the significance of inflammatory cells close to the infrainfundibulum of transitional hairs remains obscure. This study was conducted in 20 men who used a lotion containing the antimicrobials, piroctone olamine and triclosan, regularly for 18 months. At entry, mild pruritus and abnormal hair loss were reported by the volunteers. During treatments, pruritus resolved rapidly. Comparison of clinical photographs and trichograms taken at 3-month intervals suggested signs of hair regrowth with moderate increase in density of transitional hairs. Biopsies taken at 6-month intervals were processed for immunohistochemistry. There was a decrease in the density of activated T cells in the region of the follicular infrainfundibulum and isthmus over time. UgG deposits found within the epithelial sheaths at entry to the study were absent in the subsequent biopsies. The volume of the sebaceous glands, as assessed by computerized morphometry, was not modified by the treatment. It was concluded that inflammation associated with AGA transitional hairs may be driven by the presence of microorganisms harboured in the infrainfundibulum. They may modulate the severity of AGA and may potentially be controllable. This offers a new possibility of prevention and partial reversal of hair loss in AGA. [less ▲]

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See detailStrategic directions in concurrency research
Cleaveland, R.; Smolka, S. A.; Alur, R. et al

in ACM Computing Surveys (1996), 28(4), 607-625

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See detailLe campus universitaire comme espace public : des représentations aux pratiques
Frankignoulle, Pierre ULg; Bodson, Edith

in Etudes de Communication (1996), 18

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See detailTwo stage genome-wide search in inflammatory bowel disease provides evidence for susceptibility loci on chromosomes 3, 7 and 12.
Satsangi, J.; Parkes, M.; Louis, Edouard ULg et al

in Nature Genetics (1996), 14(2), 199-202

Crohn's disease (CD) and ulcerative colitis (UC), the chronic inflammatory bowel diseases (CIBD), are common causes of gastro-intestinal disease in the Western world, with a combined prevalence of 100-200 ... [more ▼]

Crohn's disease (CD) and ulcerative colitis (UC), the chronic inflammatory bowel diseases (CIBD), are common causes of gastro-intestinal disease in the Western world, with a combined prevalence of 100-200/100,000 (ref. 1). Epidemiological studies, particularly concordance rates in twin pairs and siblings, strongly implicate genetic susceptibility in the pathogenesis of CIBD. In fact, the relative contribution of genetic factors to the pathogenesis of CD may be greater than in schizophrenia, asthma or hypertension, and at least equivalent to that in insulin-dependent diabetes. Systematic screening of the entire human genome now provides a strategy for the identification of susceptibility genes in complex polygenic disorders. We undertook a two-stage genome search for susceptibility genes in inflammatory bowel disease involving 186 affected sibling pairs from 160 nuclear families. We provide strong evidence for the presence of susceptibility loci for both CD and UC on chromosome 3, 7 and 12. We obtained the highest lod score (5.47; P = 2.66 x 10(-7) with the marker D12S83 and lod scores of 3.08 and 2.69 for D7S669 and D3S1573, respectively. Our data suggest that CD and UC are closely related, but distinct, polygenic disorders that share some, but not all, susceptibility genes. [less ▲]

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See detailMeasurement of tomato firmness by using a non-destructive mechanical sensor
Lesage, Patrick; Destain, Marie-France ULg

in Postharvest Biology & Technology (1996), 8

A non-destructive mechanical sensor (Cantifruit) was designed to measure the firmness of tomatoes. It consists of a small plunger constrained to penetrate slightly into the fruits, by using an accurate ... [more ▼]

A non-destructive mechanical sensor (Cantifruit) was designed to measure the firmness of tomatoes. It consists of a small plunger constrained to penetrate slightly into the fruits, by using an accurate lever mechanism. A highly significant correlation exists between firmness measurements performed with this device and the Stable Micro Systems (SMS), fitted with the same plunger dimaeter. Using the Cantifruit, data related to firmness variability and changes are easily obtained. The firmness of tomatoes varies about 12 % around its circumference. In a single lot of tomatoes picked at the same time, the variability may exceed 25 %. If the tomatoes are stored at 4-5 °C and 92-99 % relative humidity (RH), their firmness decreases by about 20 % over ten days. [less ▲]

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See detailPhotosensitized generation of hydroxyl radical by eught new sulfur and selenium analogs of psoralen
Collet, A.; Hoebeke, Maryse ULg; Piette, J. et al

in Journal of Photochemistry and Photobiology B : Biology (1996), 35

comparative study by ESR of the hydroxyl radical generation by 8 eight new sulfur and selenium analogs of psoralen

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See detailProduction of anti-PMSG antibodies and its relation to the productivity of rabbit does
Lebas, F.; Theau-Clement, M.; Remy, Benoît ULg et al

in World Rabbit Science (1996), 4(2), 57-62

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See detailRetizonomonoletes hunanensis Fang et al. 1993 and the Retispora lepidophyta morphon.
Steemans, Philippe ULg; Fang, Xiaosi; Streel, Maurice ULg

in Inst. Géol. de l'Univ. Louvain (1996), 36

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See detailMitochondrial metabolite carrier family, topology, structure and functional properties: an overview.
Sluse, Francis ULg

in Acta Biochimica Polonica. Polish. (1996), 43(2), 349-360

A set of metabolite carriers operates the traffic of numerous molecules consumed or produced in mitochondrial matrix and/or cytosolic compartments. As their existence has been predicted by the ... [more ▼]

A set of metabolite carriers operates the traffic of numerous molecules consumed or produced in mitochondrial matrix and/or cytosolic compartments. As their existence has been predicted by the chemiosmotic theory, the first challenge, in the late sixties, was to prove their presence in the inner mitochondrial membrane and to describe the various transports carried out. The second challenge was to understand their mechanisms by the kinetic approach in intact mitochondria (seventies). The third challenge (late seventies-eighties) was to isolate and to reconstitute the carriers in liposomes in order to characterize the proteins and to establish the concept of a structural and a functional family as well as some structure-function relationship with the help of primary sequences. Genetics, molecular biology and genomic sequencing bring the fourth challenge (nineties): a raising number of putative carriers becomes known only by their primary sequences but their functions have to be discovered. The actual challenge of the future is the elucidation of the ternary structure of carrier proteins that together with site-directed mutagenesis and kinetic mechanism will permit to advance in the understanding of molecular mechanisms of transport processes. [less ▲]

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See detailHyperfine structure of Sc I by Infrared Fourier Transform Spectroscopy
Aboussaïd, A.; Carleer, M.; Hurtmans, D. et al

in Physica Scripta (1996), 53

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See detailMélanges Pierre Colman
Duchesne, Jean-Patrick ULg

in Art&fact (1996), 15

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See detailLa traversée des candiatures universitaires : Une analyse de cas
Beckers, Jacqueline ULg; Boxus, Elise ULg

in Res Academica (1996), 13(1-2), 85-104

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See detailCloning of choriocarcinoma cells shows that invasion correlates with expression and activation of gelatinase A
Crescimanno, C.; Foidart, Jean-Michel ULg; Noël, Agnès ULg et al

in Experimental Cell Research (1996), 227

Implantation and placental development are dependent upon trophoblast invasion of the endometrium. While the villous trophoblast does not display invasive behavior, the extravillous cytotrophoblast is ... [more ▼]

Implantation and placental development are dependent upon trophoblast invasion of the endometrium. While the villous trophoblast does not display invasive behavior, the extravillous cytotrophoblast is highly invasive. By cloning BeWo choriocarcinoma cells, we have isolated two distinct clones that share similarities with villous and extravillous cytotrophoblasts. When cultured at the surface of a type I collagen gel, BeWo MC-1 cells were not invasive, whereas BeWo MC-2 cells rapidly invaded this matrix. When injected subcutaneously in nude mice, BeWo MC-1 cells developed a localized tumor and BeWo MC-2 cells developed larger tumors with micrometastases. Gelatinase A expression and minute amounts of gelatinase B were detected in the parental cell line and in both clones. However, the parental and the BeWo MC-2 cells secreted 5- to 10-fold more gelatinase A than the BeWo MC-1 cells. Laminin and matrigel stimulated the production of gelatinase A in BeWo MC-2 cells. Type I collagen promoted the conversion of the 72-kDa progelatinase A in an active enzyme only in parental BeWo and in BeWo MC-2 cells. These clones provide an interesting model for studying the complex mechanisms regulating implantation as well as the controlled invasiveness during implantation compared to tumor invasion. [less ▲]

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See detailHow complex can be the Unimolecular Decomposition of a simple Molecule? The case of Acetylene. An Electron Impact and PIPECO Investigation.
Locht, Robert ULg; Servais, Christian ULg

in Zeitschrift für Physikalische Chemie (1996), 195

The dissociative ionization of C2H2, C2D2 and C2HD is presented in this work. Excepting the H2+ formation, all dissociation channels are thoroughly investigated by electron impact. The translational ... [more ▼]

The dissociative ionization of C2H2, C2D2 and C2HD is presented in this work. Excepting the H2+ formation, all dissociation channels are thoroughly investigated by electron impact. The translational energy distribution as a function of the impinging electron energy and the appearance energy as a function of the translational energy are measured for all fragment ions. KE versus AE diagrams are obtained and the isotope effect is examined. All observed thresholds are analyzed in detail and dissociation mechanisms are proposed. For the C2H+ ion the PIPECO technique has also been used. From these discussions the H-C2H, HC-CH and H-C2 binding energy values are proposed, i.e. 5.33+/-0.23 eV, 9.83+/0.10 eV and 5.44+/-0.40 eV respectively. The fragmentation path leading to C+, CH2+ and C2+ are discussed in terms of dissociation mechanisms involving the transient vinylidene structure of the molecular ion as an intermediate. [less ▲]

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See detailAnalyse de "La Mort" de Maurice Carême
Tilkin, Françoise ULg; Mathonet, Anne

in Espace pour un humanisme européen (1996), 29

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See detailLes constructions analogiques du futur III
Winand, Jean ULg

in Revue d'Egyptologie (1996), 47

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See detailAn improved preparation method of benzyl and thenyl triphenylphosphonium salt
Zhang, Jian-Xin; Dubois, Philippe ULg; Jérôme, Robert ULg

in Synthetic Communications (1996), 26(16), 3091-3095

New benzyl and thenyl phosphonium salts have been prepared by an improved method in solution from the parent alcohols and triphenylphosphine hydrobromide. Benzyl-type alcohols containing a tertiary amine ... [more ▼]

New benzyl and thenyl phosphonium salts have been prepared by an improved method in solution from the parent alcohols and triphenylphosphine hydrobromide. Benzyl-type alcohols containing a tertiary amine can also be converted within high yields to phosphonium salts by removing the formed water by azeotropic distillation. Solvent (methylene chloride, chloroform and acetonitrile) and temperature have been chosen in close relation to the alcohol reactivity. Purification is straightforward and yields are relatively high. [less ▲]

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See detailSpontaneous complete remission and recovery of donor haemopoiesis without GVHD after relapse and apparent marrow graft rejection in poor-prognosis myelodysplastic syndrome.
Beguin, Yves ULg; Collignon, Jacques ULg; Laurent, C. et al

in British Journal of Haematology (1996), 94(3), 507-9

We report a patient with poor-prognosis myelodysplastic syndrome (MDS) after successful treatment of lymphoma, who was given an allogeneic BMT, engrafted and achieved complete remission, but later had a ... [more ▼]

We report a patient with poor-prognosis myelodysplastic syndrome (MDS) after successful treatment of lymphoma, who was given an allogeneic BMT, engrafted and achieved complete remission, but later had a relapse of his MDS with complete disappearance of donor haemopoiesis. After two episodes of CMV pneumonia and continued prophylactic use of ganciclovir thereafter, he experienced a spontaneous complete disappearance of all signs of MDS, including myelofibrosis, and a complete return to donor haemopoiesis. This case is the first one to suggest a graft-versus-leukaemia effect (GVL) in MDS patients. It depicts the complex relationship between GVL, graft-versus-host disease (GVHD) and graft rejection. It could also constitute a clinical illustration of the possible antileukaemic effect of CMV infection and its treatment with ganciclovir. [less ▲]

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See detailHaemodynamic dysfunctions involved in experimental bovine pneumonic pasteurellosis
Desmecht, Daniel ULg; Amory, Hélène ULg; Linden, Annick ULg et al

in Journal of Experimental Animal Science (1996), 37

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See detailLes adénomes gonadotropes
Beckers, Albert ULg; Stevenaert, Achille ULg

in Revue Française d'Endocrinologie Clinique, Nutrition, et Métabolisme (La) (1996), 37(4-5), 299-305

Les adénomes hypophysaires gonadotropes qui sont parmi les plus fréquents, ont été identifiés récemment; on estime aujourd'hui qu'environ 80 % des adénomes hypophysaires dits non-sécrétants sont en fait ... [more ▼]

Les adénomes hypophysaires gonadotropes qui sont parmi les plus fréquents, ont été identifiés récemment; on estime aujourd'hui qu'environ 80 % des adénomes hypophysaires dits non-sécrétants sont en fait gonadotropes. Étant donné l'absente de symptomatologie spécifique, ils ne sont souvent reconnus que lorsqu'ils sont volumineux, ayant déjà entraîné un syndrome neurologique, une compression du chiasma optique. Dans la plupart des cas il est possible de les identifier grâce aux produits de sécrétion ou encore par méthodes d'immunohistochimie ou encore par la détection de l'ARN messager correspondant aux sous-unités hormonales. Le traitement reste essentiellement neuro-chirurgical (chirurgie trans-sphénoidale) car, dans la plupart des cas, les agonistes dopaminergiques et les analogues agonistes ou antagonistes de GnRH sont inefficaces. [less ▲]

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See detailThe current situation of wild mammals in Belgium: an outline
Libois, Roland ULg

in Hystrix: the Italian Journal of Mammalogy (1996), 8(1-2), 35-41

The distrbution of the wild mammals of Belgium has been intensively stutied during the years '78-81'. It was the first time that such a work was undertaken in Belgium and could lead to a better ... [more ▼]

The distrbution of the wild mammals of Belgium has been intensively stutied during the years '78-81'. It was the first time that such a work was undertaken in Belgium and could lead to a better understanding of the current status of the different species. Conservation measures were olso proposed to the political authorities who thereafter edicted a legal protection of the most vulnerable species. Unfortunately, habitat protection is rather unachieved and the situation of the most endangered species is still getting worse. [less ▲]

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See detailThe inducible 70,000 molecular/weight heat shock protein is expressed in the degenerating dentate hilus and piriform cortex after systemic administration of kainic acid in the rat.
Armstrong, J. N.; Plumier, Jean-Christophe ULg; Robertson, H. A. et al

in Neuroscience (1996), 74(3), 685-93

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic ... [more ▼]

Using both immunohistochemistry and in situ hybridization, we examined the rat brain for the expression of the inducible 70,000 mol. wt heat shock protein, Hsp70, at 3,6,12 and 24 h after systemic administration of kainic acid. In contrast to previous reports, the present study demonstrates that neurons in the regions most susceptible to seizure-induced cell death accumulate both Hsp70 messenger RNA and protein. Neurons in the denate hilus and piriform cortex contained Hsp70 messenger RNA at 6 h and protein at 12 h. These neutrons contained little or no Hsp70 messenger RNA or protein at 24 h when the majority of cells in these area were pyknotic. Injured neurons in areas such as the parietal cortex, which are less susceptible to seizure-induced cell death, expressed and maintained high levels of Hsp70 messenger RNA and protein at 12 and 24 h. This work suggest that Hsp70 messenger RNA and protein are rapidly and transiently expressed in dying neurons, and contradicts the notion that Hsp70 only accumulates in injured neurons that survive. [less ▲]

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See detailEffects of castration on fear reactions of male sheep.
Vandenheede, Marc ULg; Bouissou, Marie-France

in Applied Animal Behaviour Science (1996), 47

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See detailAssessment of nuclear totipotency of fetal bovine diploid germ cells by nuclear transfer.
Moens, A.; Chesne, P.; Delhaise, F. et al

in Theriogenology (1996), 46(5), 871-80

Nuclear transfer was used to study nuclear reprogramming of fetal diploid bovine germ cells collected at two stages of the fetal development. In the first case, germ cells of both sexes were collected ... [more ▼]

Nuclear transfer was used to study nuclear reprogramming of fetal diploid bovine germ cells collected at two stages of the fetal development. In the first case, germ cells of both sexes were collected during their period of intragonadal mitotic multiplication at 48 days post coitum (d.p.c.). In the second case, only male germ cells were collected after this period, between 105 and 185 d.p.c. Isolated germ cells were fused with enucleated oocytes. Reconstituted embryos were cultured in vitro and those reaching the compacted morula or blastocyst stage were transferred into synchronous recipient heifers. Of 511 reconstituted embryos with 48 d.p.c. germ cells (309 males and 202 females), 48% (247/511 ) cleaved; 2.7% (14/511 ) reached the compacted morula stage and 8 of them the blastocyst stage (1.6%). No difference was observed between sexes. All 14 compacted morulae/blastocysts were transferred into 6 recipients and one pregnancy was initiated. This recipient was slaughtered at Day 35 and an abnormal conceptus (extended trophectoderm and degenerated embryo) was collected. Its male sex, genetically determined, corresponded to that of donor fetus. Of 380 reconstituted embryos with male 105 to 185 d.p.c. germ cells, 72.1% (274/380 ) cleaved, 2.1% (8 380 ) reached the compact morula stage and 7 of these the blastocyst stage (1.8%). Three blastocysts and one morula were transferred into 4 recipients. Two became pregnant at Day 21 but only one at Day 35 which aborted around Day 40. Our results show that the nucleus of diploid bovine germ cells of both sexes can be reprogrammed. However, in the absence of further development of these reconstituted embryos, nuclear totipotency of bovine diploid germ cells remains to be evidenced. [less ▲]

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See detailNonspecific airway responsiveness of COPD horses : effects of environmental control
Vandenput, Sandrina ULg; Votion, Dominique ULg; Anciaux, N. et al

in Pflügers Archiv : European Journal of Physiology (1996), 431(6), 324

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See detailVers une assurance qualité intégrée de la chaîne agro-alimentaire.
Vindevogel, Henri ULg; Daube, Georges ULg; Hans, J. C. et al

in Annales de Médecine Vétérinaire (1996), 140

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See detailEffecten van internationale overnames op aandelenrendementen
Adriaanse, F.; Corhay, Albert ULg; Tourani-Rad, Alireza

in Maandblad voor Accountancy en Bedrijfseconomie [=MAB] (1996), 70(3), 123-132

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See detailGlycoprotein H (gII/gp108) and glycoprotein L form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1.
van Drunen Littel-van den Hurk, S.; Khattar, S.; Tikoo, S. K. et al

in Journal of General Virology (The) (1996), 77 ( Pt 7)

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by two ... [more ▼]

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by two different panels of monoclonal antibodies. The gII- and gp 108-specific monoclonal antibodies were shown to react with the same protein, which was identified by N-terminal sequencing as the homologue of herpes simplex virus type 1 (HSV-1) gH. When BHV-1 gH was purified by immunoadsorbent chromatography, gL was co-purified. The gH-gL complex induced the production of antibodies that neutralized virus infectivity and inhibited virus penetration. Affinity-purified gH-gL did prevent penetration, but not attachment of BHV-1, which suggests that the gH-gL complex is essential for penetration of BHV-1 into susceptible cells. [less ▲]

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See detailThe Ahrensburgian site at Zonhoven-Molenheide
Vermeersch, Pierre M.; Peleman, Chris; Rots, Veerle ULg et al

in Notae Praehistoricae (1996), 16

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See detailChantiers A et M. Stratigraphie et constructions (1989)
Limet, Henri; Tunca, Önhan ULg

in MARI 8 (1996)

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See detailMaladie de Hodgkin et facteurs de croissance
Fillet, Georges ULg

in Tempo Médical (1996), 166

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See detailDifferential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury.
Plumier, Jean-Christophe ULg; Robertson, H. A.; Currie, R. W.

in Journal of Molecular and Cellular Cardiology (1996), 28(6), 1251-60

Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer ... [more ▼]

Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer-perfused isolated rat heart after 30 min of coronary artery occlusion. The accumulation of mRNA for hsc70, hsp70, c-fos, c-jun, and Erg-1 was localized coincidently and was restricted to the ischaemic area of the heart. mRNA for these genes was undetectable at the end of the ischaemic period (no reperfusion). After 30 min of reperfusion, accumulation of mRNA for hsc70, hsp70, c-fos, and c-jun was detectable and increased with further reperfusion. Within the area labelled for these gene products was a central area of less intense labelling which corresponded to the necrotic zone. The immediate early gene product, jun-B, was localized in both the ischaemic and the non-ischaemic area of the hearts. These results suggest that the area of the heart where heat shock and immediate early gene transcripts accumulate, while injured, recovers transcriptional activity, and that the central area where minimal heat shock and immediate early gene transcripts accumulate, does not recover transcriptional activity and is irreversibly injured. [less ▲]

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See detailEffets du massage par appareil semi automatique : Etude scintigraphique et tonométrique
Crielaard, Jean-Michel ULg; Vanderthommen, Marc ULg; Dauchat, L. et al

in Kinésitherapie, les Annales (1996), 23(3), 102-105

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See detailDéveloppement actuel du concept de l'hygiène en agro-alimentaire et des exigences des normes européennes.
Vindevogel, Henri ULg; Daube, Georges ULg; Hans, J. C. et al

in Annales de Médecine Vétérinaire (1996), 140

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See detailInactivated Bovine Herpesvirus 1 Induces Apoptotic Cell Death of Mitogen-Stimulated Bovine Peripheral Blood Mononuclear Cells
Hanon, E.; Vanderplasschen, Alain ULg; Lyaku, S. et al

in Journal of Virology (1996), 70(6), 4116-4120

Bovine herpesvirus 1 (BHV-1) is able to inhibit the proliferation of bovine peripheral blood mononuclear cells. Here, we have demonstrated that live BHV-1 and, interestingly, inactivated BHV-1 can induce ... [more ▼]

Bovine herpesvirus 1 (BHV-1) is able to inhibit the proliferation of bovine peripheral blood mononuclear cells. Here, we have demonstrated that live BHV-1 and, interestingly, inactivated BHV-1 can induce apoptosis of mitogen-stimulated bovine peripheral blood mononuclear cells in vitro. [less ▲]

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See detailMagnetic texturing of bulk samples of the superconductor Bi2Sr2Ca0.8Dy0.2Cu2O8-y
Stassen, Sophie; Rulmont, André ULg; Vanderbemden, Philippe ULg et al

in Journal of Applied Physics (1996), 79(1), 553-555

The texturing process of Bi-Sr-Ca(Dy)-Cu-O (2212) is optimized by sintering under a magnetic field. The precursor crystalline powder is melted and grown under a 1.2 T magnetic field. Textured samples are ... [more ▼]

The texturing process of Bi-Sr-Ca(Dy)-Cu-O (2212) is optimized by sintering under a magnetic field. The precursor crystalline powder is melted and grown under a 1.2 T magnetic field. Textured samples are characterized by x-ray diffraction, scanning electron microscopy, electrical resistivity, and magnetic susceptibility. (C) 1996 American Institute of Physics. [less ▲]

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See detailLaparoscopic myomectomy today. Fibroids: management and treatment: the state of the art.
Donnez, Jacques; Mathieu, Pierre Emmanuel; Bassil, Salim et al

in Human Reproduction (1996), 11(9), 1837-40

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See detailTheoretical Radiative Lifetimes of 3p3(4S°)4s5S° and 3S° States in S I
Biémont, Emile ULg; Storey, P. J.; Zeippen, C. J.

in Astronomy and Astrophysics (1996), 309

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See detailUtilisation des antibiotiques dans la maladie de Crohn : de la théorie à la pratique
Louis, Edouard ULg; Drion, V.; Belaiche, Jacques ULg

in Médecine et Hygiène (1996), 54

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See detailInfluence of thermal acclimation on the survival of Sitophilus granarius (L) and Oryzaephilus surinamensis (L) at low temperatures
Mignon, Jacques ULg; Haubruge, Eric ULg; Gaspar, Charles ULg

in Netherlands Journal of Zoology (1996), 46(3-4), 317-325

Low temperatures have been used for many years to control populations of stored-product insects. The aim of aeration was primarily to cool down the grain and then to prevent its deterioration by reducing ... [more ▼]

Low temperatures have been used for many years to control populations of stored-product insects. The aim of aeration was primarily to cool down the grain and then to prevent its deterioration by reducing the number of insects. In Belgium, the mild winters enable insects to survive to the next season. In autumn, the progressive lowering of temperature has an acclimation effect on stored-product insects. The present study was undertaken to determine the survival at low temperatures of non cold-acclimated and laboratory- and field-cold-acclimated insects. We have chosen to work with the granary weevil Sitophilus granarius (L.) and the saw-toothed grain beetle Oryzaephilus surinamensis (L.). They are the most frequent stored-grain pests in Belgium. To compare the cold-hardiness of different laboratory cold-acclimated insects, S. granarius and O. surinamensis were placed at nine different cold-acclimation temperature regimes. Insects were kept at 5 degrees C for 2, 4 and 6 weeks or at -5 degrees C for 4, 7 and 14 days. To assess the field-cold-acclimation in autumn and in winter, insects were monthly taken from a bin and transferred to 5 degrees C for 6 weeks. S. granarius adults were more cold-hardy than O. surinamensis, but O. surinamensis adults compensated their cold-sensibility by a great ability to acclimate. S. granarius is able to survive the winter in Belgium because of its cold-hardiness while O. surinamensis survives because of its ability to acclimate to low temperatures. [less ▲]

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See detailBrain thiamine, its phosphate esters, and its metabolizing enzymes in Alzheimer's disease.
Mastrogiacomo, Frank; Bettendorff, Lucien ULg; Grisar, Thierry ULg et al

in Annals of Neurology (1996), 39

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See detailRecherches sur le Paléolithique supérieur de la Moldavie
Otte, Marcel ULg; López Bayón, Ignacio; Noiret, Pierre ULg et al

in Anthropologica et Praehistorica : Bulletin de la Société Royale Belge d'Anthropologie et de Préhistoire (1996), 107

The Moldavian sequence is an illustration of all the phases of the Upper Palaeolithic. It starts with archaic industries (with bifacial foliate points), then is going on with local Aurignacian industries ... [more ▼]

The Moldavian sequence is an illustration of all the phases of the Upper Palaeolithic. It starts with archaic industries (with bifacial foliate points), then is going on with local Aurignacian industries, and finally show a very intensive development of Gravettian industries. Its geographical position gives it a key-position for the study of the relationships between the hills of Central Europe and the wide eastern plains. [less ▲]

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See detailUse of the monofrequency forced oscillation technique to measure changes in upper airway resistance in Friesian and blue Belgian calves
Reinhold, Petra; Genicot, Bruno; Close, Roland et al

in Research in Veterinary Science (1996), 61(3), 227-230

Monofrequency forced oscillometry (MFO) using a sinusoidal test signal of one frequency (10 Hz) was compared with the classical pulmonary function technique (using an oesophageal balloon with airflow ... [more ▼]

Monofrequency forced oscillometry (MFO) using a sinusoidal test signal of one frequency (10 Hz) was compared with the classical pulmonary function technique (using an oesophageal balloon with airflow measurements) in two breeds of cattle which differ in their respiratory physiology, particularly to assess changes in upper airway resistance. Six healthy male Friesian calves (mean [SD] bodyweight 131 [12] kg) and six healthy male blue Belgian calves (mean [SD] bodyweight 137 [13] kg) were examined successively with their heads either in a normal position or held vertically, using a cross-over study design. In the normal position, higher resistances, a higher respiratory impedance, and lower values of the dynamic lung compliance (Cdyn) were measured in the blue Belgian than in the Friesian calves. In the vertical head position, total pulmonary resistance (RL) increased and Cdyn decreased significantly (P < 0.05). The changes in MFO-derived parameters were in accordance with the changes in classical parameters, and for both methods, the percentage changes were markedly higher in the blue Belgian calves. [less ▲]

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See detailVariations saisonnières du CO2 de l'air de deux grottes du Jura méridional: La Balme et Cerdon. Comparaison avec d'autres grottes françaises
Sbai, Abdelkader; Ek, Camille ULg; Renault, Philippe

in Annales de la Société Géologique de Belgique (1996), 119

Seasonal measurements of carbon dioxide were made over a period of one year in two caves from southern Jura: the caves of La Balme and of Cerdon. Carbon dioxide contents range from 300 to 15,500 ppm ... [more ▼]

Seasonal measurements of carbon dioxide were made over a period of one year in two caves from southern Jura: the caves of La Balme and of Cerdon. Carbon dioxide contents range from 300 to 15,500 ppm; these low values are correlated with the circulation of the air in the two caves and with the origin of the carbon dioxide. The values found in these caves are then compared with those measured by researchers in other French caves. [less ▲]

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See detailA Possible Role for the Alpha 1-->3 Galactosyl Epitope and the Natural Anti-Gal Antibody in Oncogenesis
Gollogly, L.; Castronovo, Vincenzo ULg

in Neoplasma (1996), 43(5), 285-9

Glycoconjugates and their antibodies are vital components of host-tumor interaction. This review concentrates on the oncological implications of research concerning the alpha gal triad; the alpha 1-->3 ... [more ▼]

Glycoconjugates and their antibodies are vital components of host-tumor interaction. This review concentrates on the oncological implications of research concerning the alpha gal triad; the alpha 1-->3 galactosyl epitope (alpha Gal), the enzyme responsible for its construction, alpha 1,3 galactosyl transferase (alpha 1-3GT), and its associated antibody: anti-gal. Alpha gal epitopes, previously assumed to be absent from human tissue, have been demonstrated on several human cancer cell lines, senescent red blood cells, and Graves' disease thyrocytes. Alpha-gal presence on neoplastic lines is correlated with increased metastatic formation in animal models. The mechanisms of human response to these neoantigens are complex, as natural anti-gal antibodies exist in high titers in normal sera, thus predicting immunological recognition of cells expressing alpha gal epitopes. Hypotheses vary regarding the pathogenic contributions of metastasis-associated phenomena such as de novo expression of alpha gal and its unmasking by desialylation. The means by which alpha gal is sporadically expressed in human tissue remain unknown, as the galactosyl transferase which produces this epitope in constitutively expressive animals has undergone significant mutation at the genomic level in humans. Pathological re-expression is presumed to require permissive changes at a cellular level. Detailing these alterations is a prerequisite to the comprehension of the metastatic phenotype. In this context, the possibility of therapeutic strategies affecting alpha gal expression are also discussed. [less ▲]

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See detailAnaesthesia for cardiac surgery
Larbuisson, Robert ULg; Lamy, Maurice ULg

in Current Opinion in Anaesthesiology (1996), 9

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See detailThe Appearance of CH3+ Ions from Methyl Halides by resonant photoionization. A translational energy surprisal analysis. Part I. CH3F.
Momigny, J.; Locht, Robert ULg

in Chemical Physics (1996), 206

A surprisal analysis has been applied to the translational energy distribution (TED) of CH3++F from CH3F giving rise to a new and deeper insight into the dissociation dynamics involved in the process. For ... [more ▼]

A surprisal analysis has been applied to the translational energy distribution (TED) of CH3++F from CH3F giving rise to a new and deeper insight into the dissociation dynamics involved in the process. For both the 16.5 eV and 21.22 eV photon energies used, the direct population at different energy levels of the B2E state of CH3F+ which is radiationlessly coupled to the repulsive CH3F+(A2A1) state, gives rise to wide and negatively surprised total TEDs. At both energies they have to be ascribed to severe limitations in the vibrational excitation of CH3+. Additionally, in the case of 21.22 eV photons, a non-statistical expression has to be used to account for the density of translational states. At both photon energies, the population of the CH3F+ electronic states through autoionization of Rydberg states accounts for (i) the concurrent appearance of TEDs governed by a pure RRKM behavour and (ii) the positively surprised TEDs where the angular momentum conservation plays an important role. At 16.85 eV photon energy the distributions are assigned to CH3F+ (X2E)->CH3+ (X1A'1)+F. At 21.22 eV photon energy, doubly excited electronic states of CH3F+ are assumed to take part and they are correlated with CH3+ (3A")+F channel. [less ▲]

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See detailCoulomb interaction and ferroelectric instability of BaTiO3
Ghosez, Philippe ULg; Gonze, X.; Michenaud, J. P.

in Europhysics Letters [=EPL] (1996), 33(9), 713-718

Using first-principles calculations, the phonon frequencies at the Gamma-point and the dielectric tensor are determined and analysed for the cubic and rhombohedral phases of BaTiO3. The dipole-dipole ... [more ▼]

Using first-principles calculations, the phonon frequencies at the Gamma-point and the dielectric tensor are determined and analysed for the cubic and rhombohedral phases of BaTiO3. The dipole-dipole interaction is then separated a la Cochran from the remaining short-range forces, in order to investigate their respective influence on lattice dynamics. This analysis highlights the delicate balance of forces leading to an unstable phonon in the cubic phase and demonstrates its extreme sensitivity to effective charge changes. Within our decomposition, the stabilization of the unstable mode in the rhombohedral phase or under isotropic pressure has a different origin. [less ▲]

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See detailAntithrombin III in the therapy of severe sepsis
Lamy, Maurice ULg; Eisele, B.

in Biomedical Progress (1996), 9

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See detailBulletin bibliographique de la Société Rencesvals, 1995-1996
Horrent, Jacques ULg; Henrard, Nadine ULg

in Bulletin Bibliographique de la Société Rencesvals (1996), (27), 167

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See detailEtude dynamographique de la marche de l'hémiplégique: effets de différentes modalités d'électrostimulation
Vanderthommen, Marc ULg; Lucas, C.; Laurent, J.M. et al

in Kine Acta-Symposium (1996)

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See detailRheinische Wortgeographie. Karten des Rheinischen Wörterbuchs und ihre computative Auswertung. Teil I
Lausberg, Helmut; Möller, Robert ULg

in Rheinische Vierteljahrsblätter (1996), 60

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See detailInfection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests.
Weynants, V.; Tibor, A.; Denoel, P. A. et al

in Veterinary Microbiology (1996), 48(1-2), 101-12

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of ... [more ▼]

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of brucellosis was reported in these animals and no Brucella abortus strains were isolated. After two years, no brucellosis outbreak was registered in all of the herds concerned. On this basis, all the serological reactions observed were classified as false positive. An ELISA using Yersinia Outer membrane Proteins (YOPs) as antigens was developed in order to discriminate between a Yersinia enterocolitica O:9 infection and a Brucella abortus infection. Antibodies against YOPs were detected in sera from Y. enterocolitica O:9 experimentally infected cattle (n = 4) but not in sera from B. abortus experimentally infected cattle (n = 4). In a field study, 66.7% of the 174 serum samples from cattle presenting false positive serological reactions showed anti-YOPs antibodies whereas only 10% of 454 sera, classified negative by the brucellosis screening tests, showed anti-YOPs antibodies. Our results suggest that infections with Y. enterocolitica O:9 may cause false positive reactions in brucellosis testing. [less ▲]

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See detailNew ribonucleotide reductase inhibitor, (E)-2′-deoxy-(fluromethylene) cytidine, acts as a radiosensitizer on human colon and cervix cancer cell lines
Coucke, Philippe ULg; LI, XY; Cottin, E et al

in Radiotherapy & Oncology (1996), 40(Supplément 1), 135

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See detailRecommendations for registration of drugs used in the treatment of osteoarthritis
Dougados, M; Devogelaer, JP; Annefeldt, M et al

in Annals of the Rheumatic Diseases (1996), 55

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See detailForbidden Lines in 6pk (j=1-5) configurations
Biémont, Emile ULg; Quinet, Pascal ULg

in Physica Scripta (1996), 54

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