References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailL’ostéomalacie : étiologie, clinique, physiopathologie et thérapeutique
Reginster, Jean-Yves ULg

in Le Monde Médical de la Femme (1996), 330(23), 18-21

Detailed reference viewed: 132 (3 ULg)
Full Text
Peer Reviewed
See detail9-Methoxygeissoschizol, an alkaloid from bark of strychnos guianensis
Mavar-Manga, Hélène; Quetin-Leclercq, Joëlle; Llabres, Gabriel ULg et al

in Phytochemistry (1996), 43(5), 1125-1127

The isolation and structure determination of 9-methoxygeissoschizol from the stem bark of Strychnos guianensis is described. Elucidation of its structure is mainly based on NMR studies.

Detailed reference viewed: 21 (2 ULg)
Peer Reviewed
See detailBronchopulmonary and Disseminated Granulomatous Disease Associated with Aspergillus Fumigatus and Candida Species Infection in a Golden Retriever
Clercx, Cécile ULg; Mc Entee, Kathleen ULg; Snaps, Frédéric ULg et al

in Journal of the American Animal Hospital Association (1996), 32(2, Mar-Apr), 139-45

A seven-year-old, female golden retriever was referred for a paroxysmal, chronic cough and dyspnea, dysphagia, facial pruritus, anterior uveitis, and deteriorating general condition. A severe, mixed ... [more ▼]

A seven-year-old, female golden retriever was referred for a paroxysmal, chronic cough and dyspnea, dysphagia, facial pruritus, anterior uveitis, and deteriorating general condition. A severe, mixed interstitial and alveolar pattern, with poorly defined amorphous lesions, was seen on thoracic radiographs. Multiple, whitish nodules disseminated on the hyperemic respiratory mucosa were noted on bronchoscopy. Escherichia coli and Aspergillus fumigatus were cultured from the bronchoalveolar lavage. Granulomatous lesions in numerous organs were identified during necropsy, and Aspergillus fumigatus and Candida spp. were cultured from lung and kidney tissues. Microscopic granulomatous lesions were compatible with mycotic infection; however fungal organisms were not observed. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailLes canaux potassiques ATP-dépendants et la sécrétion d'insuline: effets du BPDZ 62
Ouedraogo, R.; Pirotte, Bernard ULg; Antoine, M. H. et al

in Journal de Pharmacie de Belgique (1996), 51

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailRéactions physiologiques face à des conditions extrêmes de chaleur et d'humidité relative. Etude de la récupération après l'exercice
Art, Tatiana ULg; Votion, Dominique ULg; Lekeux, Pierre ULg

in Pratique Vétérinaire Equine (1996), 28

The purpose of this study was to follow the recovery of horses during 1hour after treadmill exercise performed either in temperated atmospheric conditions (TC) (ambient temperature: 15°C; relative ... [more ▼]

The purpose of this study was to follow the recovery of horses during 1hour after treadmill exercise performed either in temperated atmospheric conditions (TC) (ambient temperature: 15°C; relative humidity: 55%) or in hot and humid conditions (HHC) (ambient temperature: 30°C; relative humidity: 75%). Five healthy fit standardbred horses (493± 14 kg; 4± 0,5 yrs) were investigated twice at eight days interval, in TC and in HHC. Some ventilatory and cardiorespiratory measurements were made during a standardised treadmill exercise test (SET), other measurements, i.e. cardiac and respiratory frequencies and rectal temperature, during a 1-hour recovery period. The SET consisted in 8 min warm-up and in 8 min exercise (1 min at 1,7, 4, 7, 8, 9 and 10 m/sec and 2 min at 4 m/sec with a 6% (3.4°) slope). Venous blond was sampled before, 2 min, 60 min and 48 hours after the completion of the test for biochemical analysis, namely plasma electrolytes and enzymatic activities. Exercising in HHC induced a reduction of the aerobic metabolism to the total energy requierement, i.e. a 20 % decrease of VO, and 55 % increase in lactate, as well as a decrease in VE. The increase in rectal and skip temperature, the dehydration and the weight loss were higher in HHC than in TC. During the recovery period the HR and f remained higher up to 30 minutes after the end of the SET in HHC while the rectal temperature remained higher up to 60 min after the end of this test. Sa, despite the fact that our horses recovered in TC after exercising in HHC, there were significant differences between their physiological parameters measured during the recovery. This means that the recovery in air-conditionned unit would probably be an insufficient measure to ensure an adequate cooling of the horses and justify consequently the adaptations proposed by the FEI, i.e. the decrease of the distance of the run and the increase of the lime devoted to the hall between phases [less ▲]

Detailed reference viewed: 126 (3 ULg)
Full Text
Peer Reviewed
See detailPrimary Systemic Amyloidosis - A report of 2 cases
Dezfoulian, Bita ULg; Arrese Estrada, Jorge ULg; Fernandez, A. et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1996), 192

We report 2 cases of primary systemic amyloidosis. A monoclonal gammopathy was confirmed at the postmortem examination of the first patient. An extensive search for evidence of chronic infection ... [more ▼]

We report 2 cases of primary systemic amyloidosis. A monoclonal gammopathy was confirmed at the postmortem examination of the first patient. An extensive search for evidence of chronic infection, inflammation, neoplasms and paraproteinemia was conclusively negative in the other patient. The recognition of cutaneous signs of primary systemic amyloidosis is crucial to insure a rapid management aimed at postponing the fatal issue of the disease. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailNouveaux bisphosphonates dans l’ostéoporose : craintes et espoirs
Reginster, Jean-Yves ULg

in Revue du Rhumatisme (1996), 63(7-8), 545-548

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailManifestaciones cardiovasculares en el SIDA
Valdes, E. F.; Rostello, E.; Villanueva, H. R. et al

in Prensa Medica Argentina (1996), 83

Cardiovascular complications in acquired immunodeficiency syndrome. AIDS miocardiopathy has been described since 1983. Physiopathologic events leading to AIDS myocardiopathy are largely unknown: viral ... [more ▼]

Cardiovascular complications in acquired immunodeficiency syndrome. AIDS miocardiopathy has been described since 1983. Physiopathologic events leading to AIDS myocardiopathy are largely unknown: viral miocardiopathy, cytokines induced lesions, nutritional status, microvascular and ischemic miocadiopathy have been suspected. We describe a consecutive series of 50 patients (43 males, 7 women) with HIV infection and severe immunodepression (CD4<200/ml) that had clinical, ECG and echocardiographic evaluation. There were 24 drug addicted patients, 17 homosexual patients, and 10 heterosexuals. Some of these patients had the following opportunistic infections: tuberculosis (n=20), Pneumocystis carinii (n=9), Criptocococis (n=9), oropharyngeal candidosis (n=5), toxoplasmosis (n=5), histoplasmosis (n=1) and Kaposi’s sarcoma (n=2). After clinical evaluation 15/50 patients had some kind of cardiovascular complication: 6 patients had congestive heart failure, 5 patients presented with pericardial effusion, one patient has a reversible cardiac arrest, and one patient had pulmonary valvular endocarditis. After Doppler echocardiographic evaluation (according to the American echocardiography association), we found 20 patients with left ventricular diastolic dysfunction, 9 patients with right ventricular diastolic dysfunction and 6 patients with left ventricular systolic dysfunction. In conclusion, our series found left diastolic dysfunction in nearly 40 % of cases and cardiavasular events (pericarditis, heart congestive failure, arrhythmias) in 26% of cases. Cardiovascular complications have prognostic implications in AIDS and shoul be early detected. Further pathological and immunohistochemical studies are needed to clarify the phyiopathological etiologies in AIDS miocardiopathy. [less ▲]

Detailed reference viewed: 35 (7 ULg)
Peer Reviewed
See detailUne approche heuristique de l'analyse.
Hauchart, C.; Schneider, Marguerite ULg

in Repères IREM (1996), 25

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailLichens et champignons lichénicoles nouveaux ou intéressants pour la flore de Belgique et des régions voisines. VII.
van den Boom, Pieter; Diederich, Paul; Sérusiaux, Emmanuël ULg

in Bulletin de la Société des Naturalistes Luxembourgeois (1996), 97

Detailed reference viewed: 22 (0 ULg)
Peer Reviewed
See detailEnrico Mattioda, Teorie della tragedia nel settecento, Modena, Mucchi, 1994, 366
Curreri, Luciano ULg

in Franco-Italica (1996), 9

Detailed reference viewed: 22 (0 ULg)
Peer Reviewed
See detailIdentification of antigens recognized in the developing mouse brain by the RC2 antibody, a marker of radial glia
Leprince, Pierre ULg; Chanas-Sacre, G.; Wattiez, R. et al

in International Journal of Developmental Neuroscience (1996), 14(Sup. 1), 84

Detailed reference viewed: 23 (8 ULg)
Full Text
Peer Reviewed
See detailThe existence of an urban underclass in Belgium
Martiniello, Marco ULg

in New Community (1996), 22(4), 655-669

Detailed reference viewed: 19 (12 ULg)
Full Text
See detailCartes de contrôle : les cartes de Shewhart.
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1996), (2), 41

This note describes the SHEWHART control charts for subgroup means, standard deviation, medians, ranges, proportions of defectives, numbers of defectives and numbers of defects. Minitab and SAS outputs ... [more ▼]

This note describes the SHEWHART control charts for subgroup means, standard deviation, medians, ranges, proportions of defectives, numbers of defectives and numbers of defects. Minitab and SAS outputs are given for all these charts. [less ▲]

Detailed reference viewed: 49 (0 ULg)
Full Text
Peer Reviewed
See detailThe riddle of formycin A insulinotropic action
Malaisse, W. J.; Lebrun, P.; Pirotte, Bernard ULg et al

in Biochemical and Molecular Medicine (1996), 57

Detailed reference viewed: 9 (1 ULg)
Full Text
See detailCartes de contrôle : combinaison de résultats, données corrélées ou multivariées
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1996), (3), 1-37

This note first describes moving average, exponentially weighted moving average and cumulative sum control charts. Then, control charts for autocorrelated and for multivariate data are examined. Minitab ... [more ▼]

This note first describes moving average, exponentially weighted moving average and cumulative sum control charts. Then, control charts for autocorrelated and for multivariate data are examined. Minitab outputs are given for these control charts. [less ▲]

Detailed reference viewed: 34 (1 ULg)
Full Text
Peer Reviewed
See detailDu gang au groupe social: une analyse socio-préventive
Comeron, Manuel ULg

in Cahiers de la Sécurité (1996)

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de ... [more ▼]

Au delà d'une enceinte ludique, le stade de football est le théatre d'un supporterisme démonstratif: agressivité des ultra ou hooligan, exaspération partisane, foule. Le supporter est l'objet d'étude de la recherche qui évalue aussi les politiques publiques de prévention. [less ▲]

Detailed reference viewed: 69 (10 ULg)
Full Text
Peer Reviewed
See detailMuscle protein metabolism in relation to growth rate in double muscled Belgian Blue bulls: an integrated approach
Van Eenaeme, Christian ULg; Hornick, Jean-Luc ULg; Baldwin, Paule et al

in Mededelingen van de Faculteit van Landbouwwetenschappen, Rijksuniversiteit, Gent (1996), 61/4a

Detailed reference viewed: 9 (1 ULg)
Full Text
See detailLa classification numérique : principes et applications
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1996), (1), 28

The principles of some clustering techniques are described and illustrated by an example. The data consist of chemical contents for 45 types of mineral water.

Detailed reference viewed: 147 (5 ULg)
Peer Reviewed
See detailEffect Of Propylene Glycol Supplementation Around Parturition On Milk Yield, Reproduction Performance And Some Hormonal And Metabolic Characteristics In Dairy Cows
Formigoni, A.; Cornil, Mc.; Prandi, A. et al

in Journal of Dairy Research (1996), 63(1),

Detailed reference viewed: 45 (3 ULg)
Full Text
Peer Reviewed
See detailA Morphometric and Functional Study of the Toxicity of Atmospheric Ammonia in the Extrathoracic Airways in Pigs
Urbain, B.; Gustin, Pascal ULg; Charlier, G. et al

in Veterinary Research Communications (1996), 20(4), 381-399

The effects of atmospheric ammonia (NH3) on the nasal and tracheal mucosa of pigs were investigated by morphometric and functional methods. Pigs were exposed to four concentrations of NH3 [5 (control), 25 ... [more ▼]

The effects of atmospheric ammonia (NH3) on the nasal and tracheal mucosa of pigs were investigated by morphometric and functional methods. Pigs were exposed to four concentrations of NH3 [5 (control), 25, 50 and 100 ppm] for 6 days in a specially designed air-pollutant exposure chamber. Samples were taken from the turbinates and the trachea, and the respiratory mucosa was examined by light and scanning electron microscopy. Dose-response curves to carbachol and isoproterenol were constructed using isolated strips of tracheal smooth muscle, with or without epithelium. In pigs exposed to ammonia, considerable mucosal injuries were observed in the turbinates but not in the trachea. The number of neutrophils in the epithelial layer and in the lamina propria, and epithelial hyperplasia were closely and significantly correlated with the concentrations of ammonia (r = 0.894, p < 0.001; r = 0.727, P < 0.001; and r = 0.818, p < 0.001, respectively). Except for the lamina propria, all these changes were significant (p < 0.05) at ammonia concentrations as low as 25 ppm. The percentage of the surface of the turbinate mucosa that was ciliated tended to decrease with increasing ammonia concentration (r = 0.439, p < 0.082). Ammonia induced smooth-muscle hyperresponsiveness to carbachol with a close linear correlation between individual values of the carbachol-induced maximal effect and the NH3 concentrations (r = 0.526, p < 0.003). While mechanical destruction of the epithelium induced an increase in Emax in the control group, no difference was observed between the intact and denuded strips from animals exposed to ammonia. The response to isoproterenol was not influenced by ammonia. It was concluded that quantitative histological analysis of the inflammatory infiltration and epithelial hyperplasia in the turbinates is a useful tool for quantifying the effects of atmospheric pollutants in pigs; a 6-day exposure to ammonia induces nasal irritation and functional disturbances of the tracheal smooth-muscle contractions at concentrations as low as 25 ppm [less ▲]

Detailed reference viewed: 14 (0 ULg)
Peer Reviewed
See detailCompte rendu de l'ouvrage de P. Contamine, Des pouvoirs en France. 1300/1500
Marchandisse, Alain ULg

in Le Moyen Âge (1996), 102

Detailed reference viewed: 10 (1 ULg)
See detailLa nutrition et le couple "mère-enfant"
Hennart, P; Porignon, Denis ULg; Donnen, P

in Académie Royale des Sciences d'Outre-Mer (1996)

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailIs IL-6 capable to modify chondrocyte metabolism ?
Henrotin, Yves ULg; Labasse, A; Jaspar, JM et al

in Clinical Rheumatology (1996), 15

Detailed reference viewed: 3 (2 ULg)
Full Text
See detailDéplacements prémigratoire et itinéraire de migration d'une cigogne noire (Cicinia nigra) juvénile suivie par télémétrie satellitaire
Libois, Roland ULg; Jadoul, Gérard

in Aves (1996), 33(1), 1-10

On 19th of July 1995, a juvenile black stork was caught in the vicinity of Bastogne (Province of Luxembourg, Belgium) and was fitted a PTT platform weighing about 75 grams. The locations obtained by the ... [more ▼]

On 19th of July 1995, a juvenile black stork was caught in the vicinity of Bastogne (Province of Luxembourg, Belgium) and was fitted a PTT platform weighing about 75 grams. The locations obtained by the Argos system were sorted according to their precision and the fixes of good quality (A,0,2 or 3 in the Argos classification) were mapped. From the 19th July to the first migration day (28 August at 09h GMT), more than fifty percent of the 109 obtained fixes are located inside a zone of 100 km² (5.65 km radius) centered near the capture point. Moreover, that zone bas been visited nearly every day by the bird. Twenty more percent of the fixes are situated in a wider area of 300 km² (9.77 km radius). That region is covered by spruce plantations, deciduous forests and cattle meadows with numerous small watercourses and several ponds including fish ponds. Some medium distance (10 to 110 km apart from the home range centre) movements were also recorded, mainly eastwards and westwards. No difference has been evidenced in the home range use during the 4 decades of monitoring. The migration travel was monitored till the 06th September, when the transmitter failed due to a technical misconception. At that time, the stork was located in the region of Fès (Morocco). Within 9 days, the bird has travelled a distance of ca 2,400 km (mean daily movement: 237 km). The Pyrenees were crossed in the high valley of the river Tech (Pyrénées Orientales, France) only 3 days after the departure and the bird has been seen by Spanish colleagues when flying across the strait of Gibraltar (Tarifa, 5 September, 07.55 h GMT). [less ▲]

Detailed reference viewed: 27 (3 ULg)
See detailGaston Leroux et la dissociation de la matière romanesque
Denis, Benoît ULg

in Tapis-franc. Revue du roman populaire (1996), (7), 56-66

Detailed reference viewed: 28 (0 ULg)
Peer Reviewed
See detailL’immigration. De l’homme seul à la famille de A. Zerhaoui
Martiniello, Marco ULg

in Revue Européenne des Migrations Internationales (1996), 12(1), 224-225

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailEndometrial laser interstitial hyperthermy: a potential modality for endometrial ablation.
Donnez, Jacques; Polet, Roland; Mathieu, Pierre-Emmanuel et al

in Obstetrics & Gynecology (1996), 87(3), 459-64

This study was undertaken to evaluate the efficacy and safety of an intrauterine device designed for endometrial ablation by Nd-YAG laser interstitial hyperthermy. Eight hysterectomy specimens were ... [more ▼]

This study was undertaken to evaluate the efficacy and safety of an intrauterine device designed for endometrial ablation by Nd-YAG laser interstitial hyperthermy. Eight hysterectomy specimens were treated in vitro with an output power of 30 W for 5 minutes; tissue temperatures, recorded by thermocouples, were plotted to draw time-temperature and time-distance curves. As a preliminary series, ten patients were treated, with the first five subjected to local temperature monitoring; all ten were followed-up for 6-17 months. Immediate and delayed (6 weeks) histologic data were obtained. The intramural temperature at 6 mm from the fibers ranged from 55-60C). In vivo, the cooling effect of the arterial blood flow lowered the serosal temperatures under 41C. The histologic data and the hysterographic pictures suggest that endometrial ablation was effective. This new device does not require distending medium, hysteroscopic control, or high-powered Nd-YAG laser machines. Preliminary long-term results suggest that the device could be used for endometrial ablation. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailA double-blind, three-week study to compare the efficacy and safety of meloxicam 7.5 mg and meloxicam 15 mg in patients with rheumatoid arthritis.
REGINSTER, Jean-Yves ULg; Distel, M.; Bluhmki, E.

in British Journal of Rheumatology (1996), 35(Suppl 1), 17-21

A multicentre, double-blind, randomized study was conducted in patients with rheumatoid arthritis (RA) in order to compare the efficacy and safety of two different doses of meloxicam, a new preferential ... [more ▼]

A multicentre, double-blind, randomized study was conducted in patients with rheumatoid arthritis (RA) in order to compare the efficacy and safety of two different doses of meloxicam, a new preferential cyclooxygenase-2 (COX-2) inhibitor. Four hundred and twenty-three patients were randomized to receive once-daily oral meloxicam 7.5 mg (n = 216) or meloxicam 15 mg (n = 207) for 3 weeks. The Ritchie joint index and pain in the morning were significantly improved versus baseline (P < 0.001) in both groups. There were no significant differences between the effects of each dose with respect to these measures nor with respect to final assessment of global efficacy by the patients. However, the 15 mg dose was associated with a significantly (P < 0.05) better effect on morning stiffness and grip strength. No differences between the doses were observed with regard to the other secondary efficacy parameter (pain at night, body weight and erythrocyte sedimentation rate). Both doses of meloxicam were well tolerated. There were no differences between the doses with respect to global tolerance as assessed by the patient and the patients, 'general condition'. In conclusion, meloxicam at a once-daily dose of either 7.5 or 15 mg is well tolerated and effective in the treatment of patients with RA. [less ▲]

Detailed reference viewed: 18 (4 ULg)
Peer Reviewed
See detailCompte rendu de J.-L. LEMAITRE, Le Limousin monastique
George, Philippe ULg

in Moyen Age (Le) (1996)

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailType II to type I transformation of chronically stimulated goat latissimus dorsi muscle: a histoenzymological, biochemical, bioenergetic, and functional study.
Radermacker, M. A.; Focant, B.; Hautecler, T. et al

in European Surgical Research = Europäische Chirurgische Forschung = Recherches Chirurgicales Européennes (1996), 28

Five goat latissimus dorsi muscles (LDM) were submitted to a progressive chronic electrostimulation program to reach an integrated understanding of the fast-to-slow transformation process in large mammals ... [more ▼]

Five goat latissimus dorsi muscles (LDM) were submitted to a progressive chronic electrostimulation program to reach an integrated understanding of the fast-to-slow transformation process in large mammals. LDM were regularly sampled and followed during a period of 8 months. Each sample was simultaneously assessed for histoenzymological study, myosin and LDH isoforms and bioenergetic capacities [NADH dehydrogenase cytochrome c oxidoreductase (NADH Cyt c OR), succinate dehydrogenase cytochrome c oxidoreductase (Succ Cyt c OR), cytochrome c oxidase (Cyt c Ox) and LDH]. Such muscles were also tested with and without completion of II to I transformation for their mechanical properties in isometric and isotonic strain gauge testing. The conversion of fast-to-slow myosin monitored by heavy chain (HC I) and light chain slow component (LC2s) began a few days after stimulation and was almost 100% after 100 days. The H-LDH isoforms evolved similarly but did not reach 100% conversion after 200 days. The activity of respiratory chain oxidases increased within 36 h but to a variable extent and peaked after 32 days, corresponding to a 75% transformation of myosin compared to initial levels. NADH Cyt c OR, Succ Cyt c OR, and Cyt c Ox, respectively increased 10-, 5- and 5-fold. These activities then significantly decreased before the completion of the myofibrillar transformation and reached a plateau with stable activities that remained 2- to 3-fold higher than the unstimulated LDM. LDH activity sharply decreased until day 62 (5-fold) and then plateaued. Functionally, muscle showed a reduced speed of contraction and moderate reduction in power output but had become fatigue-resistant. This study documents the transformation process in large mammals and suggests the dynamic relation between workload, aerobic-anaerobic metabolism and the contractile myofibrillar system. [less ▲]

Detailed reference viewed: 18 (7 ULg)
Full Text
Peer Reviewed
See detailThe ovarian follicle in cow: in vivo growth and in vitro culture
Beckers, Jean-François ULg; Drion, Pierre ULg; Figueiredo, J. R. et al

in Reproduction in Domestic Animals (1996), 31

Detailed reference viewed: 82 (10 ULg)
Peer Reviewed
See detailMorphometric, immunohistological and three-dimensional evaluation of the endometrium of menopausal women treated by oestrogen and Crinone, a new slow-release vaginal progesterone.
Casanas-Roux, Françoise; NISOLLE, Michelle ULg; Marbaix, Etienne et al

in Human Reproduction (1996), 11(2), 357-63

Recently advanced computerized technology was applied to the investigation of morphometric, immunohistological and three-dimensional changes of the endometrial mucosa in order to evaluate quantitatively ... [more ▼]

Recently advanced computerized technology was applied to the investigation of morphometric, immunohistological and three-dimensional changes of the endometrial mucosa in order to evaluate quantitatively the effects of three doses of a new slow-release vaginal progesterone on the endometrium in post-menopausal women. A total of 20 menopausal women, deprived of ovarian function, were given oestrogen for 12 days and a combined therapy of oestrogen (administered orally) and progesterone for another 12 day period. Progesterone was administered vaginally through a new gel (Crinone) utilizing a bioadhesive, biocompatible polymer as a base to achieve a sustained release effect. An endometrial biopsy was taken before treatment, after oestrogen-only treatment and after the oestro-progestogen therapy. Before treatment, all the patients exhibited an atrophic endometrium. After oestrogen-only treatment, typical proliferative changes occurred: an increase in the endometrium thickness, an increase in the mitotic index, numerous cylinder-like glands and no coiled glands, and high concentrations of oestrogen receptors (ER) and progesterone receptors (PR). After the oestro-progestogen therapy, whatever the dose of progesterone given, a secretory transformation of the endometrial mucosa occurred, mitotic activity decreased significantly, more ramified and coiled glands were observed, and a decrease in PR content was noted in epithelial and stromal nuclei, and a decrease in PR content was also observed in epithelial nuclei but not in stromal nuclei. Accurate new techniques of image analysis have shown that crinone therapy could eliminate the proliferative effects of oestrogen treatment in post-menopausal women, despite doses as low as 45 mg of progesterone administered vaginally every other day. The results suggest that the sustained release effects of Crinone are clinically relevant. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailGlucose, alpha-amino nitrogen and amino acid exchange across the hindlimb in young bulls maintained at two growth rates
Hornick, Jean-Luc ULg; Van Eenaeme, Christian ULg; Gauthier, Sabine et al

in Canadian Journal of Animal Science (1996), 76(2), 193-202

The effect of growth rate and protein supplementation on muscle metabolism of eight bulls from the Belgian Blue breed, double-muscled type, was investigated by the arterio-venous difference technique. A ... [more ▼]

The effect of growth rate and protein supplementation on muscle metabolism of eight bulls from the Belgian Blue breed, double-muscled type, was investigated by the arterio-venous difference technique. A low growth (LG) group was maintained at a low growth rate over 36 d, and a rapid growth (RG) group for 28 d before receiving a fattening diet allowing for a rapid growth. At the end of the RG period the RG bulls received a supplement of protected soybean meal. Animals were fitted with an aortic ultrasonic blood flow probe and with catheters in the aorta and the vena cava. The blood flow in the hindlimbs of bulls varied greatly by time of the day but was higher in the RG group. The RG group had a higher arterio-venous difference (AVD) and uptake of alpha-amino nitrogen while AVD in essential amino acids was four times higher and uptake eight times higher. Significant higher AVD or uptake was observed in individual amino acids such as leucine, isoleucine and lysine. The supplementation with protected soybean meal had significant negative effect on the uptake of several amino acids. It was concluded that caution should be exercised when measuring punctually blood flow in muscle tissue, for example by dilution techniques. At high growth rate, the requirements for amino acids are larger than for glucose. Excess protein provides no additional benefit. [less ▲]

Detailed reference viewed: 23 (0 ULg)
Peer Reviewed
See detailSaint Remacle, évangélisateur en Ardenne (ca. 650). Mythe et réalité
George, Philippe ULg

in Bulletin de l'Institut Historique Belge de Rome (1996), XXXVIII

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailThree hTIM mutants that provide new insights on why TIM is a dimer
Mainfroid, Véronique; Terpstra, Peter; Beauregard, Marc et al

in Journal of Molecular Biology (1996), 257(2), 441-56

Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced, in ... [more ▼]

Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced, in which a glutamine residue was substituted for either Met14 or Arg98, both of which are interface residuces. These substitutions strongly interfere with TIM subunit association, since these mutant TIMs appear to exist as compact monomers in dynamic equilibrium with dimers. In kinetic studies, the M14Q mutant exhibits significant catalytic activity, while the R98Q enzyme is inactive. The M14Q enzyme is nevertheless much less active than unmutated hTIM. Moreover, its specific activity is concentration dependent, suggesting a dissociation process in which the monomers are inactive. In order to determine the conformational stability of the wild-type and mutant hTIMs, unfolding of all three enzymes was monitored by circular dichroism and tryptophan fluorescence spectroscopy. In each case, protein stability is concentration dependent, and the unfolding reaction is compatible with a two-state model involving the native dimer and unfolded monomers. The conformational stability of hTIM, as estimated according to this model, is 19.3 (+/-0.4) kcal/mol. The M14Q and R98Q replacements significantly reduce enzyme stability, since the free energies of unfolding are 13.8 and 13.5 (+/- 0.3) kcal/mol respectively, for the mutants, A third mutant, in which the M14Q and R98Q replacements are cumulated, behaves like a monomer. The stability of this mutant is not concentration-dependent, and the unfolding reaction is assigned to a transition from a folded monomer to an unfolded monomer. The conformational stability of this double mutant is estimated 2.5 (+/-0.1) kcal/mol. All these data combined suggest that TIM monomers are thermodynamically unstable. This might explain why TIM occurs only as a dimer. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailParadoxical Sleep Deprivation Increases the Content of Glutamate and Glutamine in Rat Cerebral Cortex
Bettendorff, Lucien ULg; Sallanon-Moulin, M.; Touret, Monique et al

in Sleep (1996), 19(1), 65-71

We investigated the influence of the sleep/waking cycle, the effects of paradoxical sleep deprivation (PSD) and of the vigilance-promoting drug modafinil on the amino acid contents of rat brain cortex. No ... [more ▼]

We investigated the influence of the sleep/waking cycle, the effects of paradoxical sleep deprivation (PSD) and of the vigilance-promoting drug modafinil on the amino acid contents of rat brain cortex. No significant nycthemeral variations in amino acid levels could be detected. PSD (12-24 hours), using the water tank method, significantly increased the levels of glutamate and glutamine. The increase was still observed after the sleep rebound period. gamma-Aminobutyric acid (GABA) levels did not change significantly during the instrumental sleep deprivation but increased during the rebound period. Control experiments indicate that the increase in glutamate and glutamine levels is due to PSD rather than to the stress associated with the experimental procedure. The increase in glutamate content cannot arise only from transamination reactions, because the levels of other amino acids (such as aspartate) did not decrease. Modafinil treatment did not significantly modify the brain cortex content of any of the amino acids tested. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Peer Reviewed
See detailLe chaponnage, une technique qui revient à la mode!
Marlier, Didier ULg; Vindevogel, Henri ULg

in Annales de Médecine Vétérinaire (1996), 140

Detailed reference viewed: 480 (0 ULg)
Full Text
Peer Reviewed
See detailDéveloppement d'un modèle de type arbre appliqué à la croissance des peuplements feuillus irréguliers du sud de la Belgique
Lejeune, Philippe ULg

in Canadian Journal of Forest Research = Journal Canadien de la Recherche Forestière (1996), 26(10), 1838-1848

A model predicting circumference growth of individual trees has been developed for mixed irregular stands dominated by Fagus silvatica L. in southeastern Belgium. This model integrates simultaneously tree ... [more ▼]

A model predicting circumference growth of individual trees has been developed for mixed irregular stands dominated by Fagus silvatica L. in southeastern Belgium. This model integrates simultaneously tree, stand, and growing site characteristics without considering distances between individual trees. It has a determination coefficent of 40.1 % and a residual standard deviation of 0.45cm/year. Forest mensuration variables considered in the model are circumference, tree social position represented by the total basal area of trees greater than the subject tree, stand basal area, and some index of stand structure corresponding to the ratio of the variance over the mean circumference. The site component is essentially expressed by the length of the growing period. The low accuracy obtained for individual tree growth is relative because the results are used mainly after individual trees have been distributed into size classes. We have been able to show that the introduction of crown description in the model can increase its accuracy. But this modification requires a submodel describing crown changes over time. [less ▲]

Detailed reference viewed: 18 (5 ULg)
Peer Reviewed
See detailCloning and Sequencing of the Dnak Locus in Streptomyces Coelicolor A3(2)
Brans, Alain ULg; Loriaux, Axelle; Joris, Bernard ULg et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1996), 6(3), 179-84

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK ... [more ▼]

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively. [less ▲]

Detailed reference viewed: 20 (7 ULg)
Full Text
Peer Reviewed
See detailInterleukine 1 et métabolisme osseux
Reginster, Jean-Yves ULg; Zheng, SX

in Revue du Praticien (La) (1996), 46

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailWhat about lorisk Figo Stage Ia and Ib, G1-G2 endometrial adenocarcinoma ?
Delaloye, J-F; Megalo; COUCKE, Philippe ULg et al

in Radiotherapy & Oncology (1996), 43(Supp 1), 6

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailSensitivity calculations and variance analysis in plant measurement reconciliation
Heyen, Georges ULg; Marechal, Eric; Kalitventzeff, Boris ULg

in Computers & Chemical Engineering (1996), 20(Suppl. A), 539-544

Analysis of the results of a data reconciliation program is made easier by extracting more information from the Jacobian matrix of the constraint equations. Standard deviation for all state variables ... [more ▼]

Analysis of the results of a data reconciliation program is made easier by extracting more information from the Jacobian matrix of the constraint equations. Standard deviation for all state variables (measured or not measured) is related to the standard deviation of measurements. Distinction between variables that are actually corrected by the validation process, and those that are merely derived from a single measurement is straightforward. Based on this information, decisions can be taken : deletion of unnecessary measurements, addition of new measurement points and their optimal selection, or identification of key measurements for which any enhancement of accuracy would result in significant improvement in the quality of the process validation. [less ▲]

Detailed reference viewed: 29 (5 ULg)
Peer Reviewed
See detailComparative genome organization of vertebrates. The First International Workshop on Comparative Genome Organization.
Andersson, L.; Archibald, A.; Ashburner, M. et al

in Mammalian Genome : Official Journal of the International Mammalian Genome Society (1996), 7(10), 717-34

Detailed reference viewed: 4 (1 ULg)
Full Text
Peer Reviewed
See detailLocal orders in II-VI liquid compounds
Gaspard, Jean-Pierre ULg; Raty, Jean-Yves ULg; Ceolin, R. et al

in Journal of Non-Crystalline Solids (1996), 205-207

The structure of liquid CdTe, HgTe and ZnTe is studied by neutron scattering. Upon melting, drastically different behaviors are found: CdTe and ZnTe keep their fourfold coordination in the melt and remain ... [more ▼]

The structure of liquid CdTe, HgTe and ZnTe is studied by neutron scattering. Upon melting, drastically different behaviors are found: CdTe and ZnTe keep their fourfold coordination in the melt and remain semiconductors, whereas HgTe becomes sixfold coordinated and metallic. This dissimilarity is discussed in terms of the electronegativity difference and of the repulsive potential hardness. Entropic aspects are also discussed [less ▲]

Detailed reference viewed: 15 (0 ULg)
Peer Reviewed
See detailAu fur et à mesure qu'elles se construisent, les trajectoires professionnelles divergent entre les sexes : l'exemple belge
Gavray, Claire ULg

in Formation Emploi : Revue Française de Sciences Sociales (1996), 93

Detailed reference viewed: 15 (2 ULg)
Peer Reviewed
See detailVardaman's Fish and Addie's Jar: Faulkner's Tales of Mourning and Desire
Delville, Michel ULg

in HJEAS, Hungarian Journal of English and American Studies (1996)

Detailed reference viewed: 82 (2 ULg)
Peer Reviewed
See detailComparison of food conversion by the Pirapatinga Piaractus brachypomus under different times of feeding
Baras, E.; Mélard, Charles ULg; Grignard, J. C. et al

in Progressive Fish-Culturist (1996), 58

Detailed reference viewed: 19 (3 ULg)
Peer Reviewed
See detailIn vitro activity of amoxycillin/clavulanate and ticarcillin/clavulanate compared with that of other antibiotics against anaerobic bacteria: comparison with the results of the 1987 survey
Pierard, denis; De Meyer A; Rosseel, P. et al

in Acta Clinica Belgica (1996), 51(2), 70-9

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight ... [more ▼]

The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight Belgian university hospitals and compared with that of 8 other antibiotics using the NCCLS reference agar dilution procedure. Production of beta-lactamase was detected by the nitrocefin test in 48% of the isolates. At NCCLS-recommended breakpoints, more than 90% of isolates were susceptible to amoxycillin/clavulanate, ticarcillin/clavulanate, piperacillin/tazobactam, imipenem, chloramphenicol and metronidazole but only 77%, 72% and 48% to cefoxitin, clindamycin and penicillin, respectively. In comparison with the results of a similar survey conducted in 1987 no major changes in susceptibility were observed except for the susceptibility to clindamycin that declined from 83% to 72% overall, and from 83% to 66% in the B. fragilis group. Furthermore one isolate of Clostridium clostridioforme was found produce beta-lactamase and few B. fragilis group isolates showed reduced susceptibility to metronidazole. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailPrediction of signal peptide functional properties: a study of the orientation and angle of insertion of yeast invertase mutants and human apolipoprotein B signal peptide variants.
Talmud, P.; Lins, Laurence ULg; Brasseur, Robert ULg

in Protein Engineering (1996), 9(4), 317-21

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using ... [more ▼]

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using molecular modelling of the invertase signal peptide we have analysed the hydrophobicity potential and the change in dielectric constant of the energy transfer, when the molecule moves from a hydrophobic to a hydrophilic phase at the simulated hydrophobic-hydrophilic interface. This modelling has been carried out on wild type and mutant invertase signal peptides of altered function, previously reported in the literature. While the predicted angle of insertion correlates with the measured extent of invertase secretion, with an optimum angle of 45 degrees, mutations that change the angle of orientation reduce the extent of invertase secretion. We have applied these same molecular modelling principles to the naturally occurring variants of the human apolipo-protein B (apoB) signal peptide, that confer a secretion defective phenotype when fused to yeast invertase and expressed in yeast. Our modelling thus identifies a strong correlation between the predicted angle of insertion of the signal peptide into the membrane and its ability to direct secretion. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailEffect Of Oral And Parenteral N Nutrition Vs N-Free Nutrition On The Endogenous Amino Acid Flow At The Ileum Of The Pig
Leterme, Pascal; Monmart, Thierry; Thewis, André ULg et al

in Journal of the Science of Food and Agriculture (1996), 71(2), 265-271

Two methods were tested for suppressing the depressive effect of N-free diets on the digestive secretions in pigs: the blood perfusion of amino acids (AA) or the peptide alimentation method. In the latter ... [more ▼]

Two methods were tested for suppressing the depressive effect of N-free diets on the digestive secretions in pigs: the blood perfusion of amino acids (AA) or the peptide alimentation method. In the latter, enzymically hydrolysed casein (EHC), composed of oligopeptides and free AA, was used as the source of nitrogen. The unabsorbed dietary N molecules were discarded from the ileal digesta by ultrafiltration or gel filtration, assuming that the endogenous fraction did not contain significant amounts of small molecules. The AA supply by blood perfusion had no effect on the ileal endogenous AA losses (8.0 g AA kg-' DM intake) in growing pigs (k 50 kg), compared with the N-free diet alone (8.3 g), whereas the EHC supplementation significantly increased them (18.0 8). The increase was due to both endogenous and dietary N. The presence of unabsorbed dietary AA in the ileal digesta was confirmed by the AA profile of the soluble molecules with a very low molecular mass (< 3 kDa), which was close to that of EHC. Both ultrafiltration (cut-offs of 3 or 10 kDa) and gel filtration methods, utilised to discard the remaining dietary molecules, also eliminated a significant proportion of endogenous AA. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailEffect of total treatment time on event-free survival in carcinoma of the cervix
Delaloye, J-F; COUCKE, Philippe ULg; Pampallona, S et al

in Gynecologic Oncology (1996), 60

Detailed reference viewed: 6 (1 ULg)
See detailPrécarité psycho-sociale différentielle des hommes et des femmes
Born, Michel ULg; Gavray, Claire ULg

in Cahiers de l'IPSA (1996), 19

Detailed reference viewed: 34 (6 ULg)
Peer Reviewed
See detailEffets de la PEEP sur les propriétés hémodynamiques du réseau artériel systémique
LAMBERMONT, Bernard ULg; DETRY, Olivier ULg; fossion, A et al

in Réanimation (1996), 4

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailThe Erythrocyte/Brain Glucose Transporter (Glut1) May Adopt A Two-Channel Transmembrane Alpha/Beta Structure.
Ducarme, P.; Rahman, M.; Lins, Laurence ULg et al

in Journal of Molecular Modeling (1996), 2(2), 27-45

There are two models of topology for the membrane domains of the erythrocyte/brain facilitative glucose transporter, GLUT1. The first is composed of 12 membrane-spanning a-helices, the second of 16 ... [more ▼]

There are two models of topology for the membrane domains of the erythrocyte/brain facilitative glucose transporter, GLUT1. The first is composed of 12 membrane-spanning a-helices, the second of 16 membrane-spanning b-strands. We have used Jähnig’s and Eisenberg’s methods to identify possible transmembrane segments (10 spanning a-helices and 4 b-strands). The topology proposed is more consistent with available experimental data from FTIR, CD and mapping experiment than the previous models . We suggest that GLUT1 might form two channels, one of which is responsible for glucose transport. This agrees with the theoretical and experimental arguments. Finally, an analysis of the mutation periodicity and of the mean hydrophobicity for the GLUT family is provided in order to evaluate the packing of the protein in the membrane. Keywords: [less ▲]

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailBacteriogical evidence of leptospira infetion in wild mammals from Azores archipelago, Portugal (short report)
Collares Pereira, Margarida; Santos Reis, Margarida; Oom, Maria do Mar et al

in Vie et milieu (1996), 46(3-4), 380-381

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailStructure-specific nuclease activity in yeast nucleotide excision repair protein RAD2
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1995), 270(50), 30194-8

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailDynamic properties of the transient network formed by telechelic ionomers studied by dynamic light scattering and dynamic mechanical analysis
Johannsson, Ragnar; Chassenieux, Christophe; Durand, Dominique et al

in Macromolecules (1995), 28(25), 8504-8510

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L ... [more ▼]

Solutions of alpha,omega-sodium sulfonatopolyisoprene (alpha,omega NaPIPS) in toluene are studied using static and dynamic light scattering and dynamic mechanical analysis. At concentrations above 4 g/L, which is well below the overlap concentration of the polymer chains (C*), a transient network is formed characterized by a plateau modulus at high frequencies and a relatively narrow viscoelastic relaxation time distribution. The intensity autocorrelation function of the transient network shows two relaxational modes: a relatively fast diffusional mode close to the co-operative diffusional mode of semidilute solutions of the unfunctionalized polymers and a slow broader mode which becomes independent of the scattering angle at a concentrations close to C*. The relation between this slow mode and the viscoelastic relaxation is discussed. Both the slow mode and the viscoelastic relaxation processes have an Arrhenius temperature dependence with the same activation energy, E(a) = 25 kJ/mol. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Peer Reviewed
See detailThe robustness of self-organized criticality against extinctions in a tree-like model of evolution
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Europhysics Letters [=EPL] (1995), 32(7), 613-618

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the ... [more ▼]

We investigate a so-called punctuated-equilibrium model of tree-like evolution containing extinctions for the weakest species with respect to a strength parameter r. Without extinctions (for r = 0), the model leads to self-organized criticality. For r not equal 0, a transition from growing trees to finite ones takes place at some critical r(c) = 0.48 +/- 0.01 value. For 0 less than or equal to r less than or similar to r(c), self-organized criticality is thus robust against extinctions of the weakest species. The size distribution of avalanches follows a power law behaviour with an exponent 3/2 which seems to be independent of the parameter r. The growing trees are found to be self-similar with a non-universal fractal dimension D-f non-trivially ranging from 2 to 1 depending on the parameter r. This constraint model opens up the field of description of various possible physical events for such an evolution model. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailEIT: Extreme-Ultraviolet Imaging Telescope for the SOHO Mission
Delaboudinière, J.-P.; Artzner, G. E.; Brunaud, J. et al

in Solar Physics (1995), 162

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer ... [more ▼]

The Extreme-ultraviolet Imaging Telescope (EIT) will provide wide-field images of the corona and transition region on the solar disc and up to 1.5 Ro above the solar limb. Its normal incidence multilayer-coated optics will select spectral emission lines from Fe IX (171 Å), Fe XII (195 Å), Fe XV (284 Å), and He II (304 Å) to provide sensitive temperature diagnostics in the range from 6 × 10[SUP]4[/SUP] K to 3 × 10[SUP]6[/SUP] K. The telescope has a 45 x 45 arcmin field of view and 2.6 arcsec pixels which will provide approximately 5-arcsec spatial resolution. The EIT will probe the coronal plasma on a global scale, as well as the underlying cooler and turbulent atmosphere, providing the basis for comparative analyses with observations from both the ground and other SOHO instruments. This paper presents details of the EIT instrumentation, its performance and operating modes. [less ▲]

Detailed reference viewed: 53 (6 ULg)
Full Text
Peer Reviewed
See detailPre- and Post-Translational Regulation of Aromatase by Steroidal and Non-Steroidal Aromatase Inhibitors
Foidart, Agnès ULg; Tlemcani, O.; Harada, N. et al

in Brain Research (1995), 701(1-2), 267-78

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir ... [more ▼]

Treatment of castrated quail with testosterone (T) reliably activates male copulatory behavior and, at the same time, increases the aromatase activity (AA), the number of aromatase-immunoreactive (ARO-ir) cells and the concentration of aromatase mRNA as measured by RT-PCR in the brain. All these effects can be mimicked by estrogens. The behavioral effects of T can be blocked by a variety of aromatase inhibitors and, in parallel, the AA is strongly inhibited in the preoptic area (POA). We showed recently that the steroidal inhibitor, 4-OH-androstenedione (OHA) markedly decreases the immunostaining density of brain ARO-ir cells while the non-steroidal inhibitor, R76713 (racemic Vorozole; VOR) unexpectedly increased the density of this staining, despite the fact that the enzyme activity was completely inhibited. To generalize these findings and try to identify the underlying mechanism, we compared here the effects of two steroidal (OHA and androstatrienedione [ATD]) and two non-steroidal (VOR and Fadrozole [FAD]) aromatase inhibitors on the aromatase immunostaining and aromatase mRNA concentration in the brain of castrated quail concurrently treated with T. The 4 inhibitors significantly blocked the activation by T of male copulation. The two steroidal inhibitors decreased the immunostaining of brain ARO-ir cells but both VOR and FAD markedly enhanced the density of this staining. In parallel, OHA and ATD completely blocked the T-induced increase in aromatase mRNA concentration, while VOR and FAD had no effect on these RNA concentrations in the POA-anterior hypothalamus and they decreased them only slightly in the posterior hypothalamus. Taken together these results suggest that the inhibition of AA by ATD or OHA and the subsequent removal of locally produced estrogens blocks the synthesis of aromatase presumably at the transcriptional level. By contrast, the two non-steroidal inhibitors tested here block AA but in parallel increase the aromatase immunostaining. This effect does not result from an enhanced transcription and it is therefore speculated that these compounds increase either the translation of the aromatase mRNA or the half-life of the protein itself. [less ▲]

Detailed reference viewed: 20 (1 ULg)
See detailLes clivages entérinés dans la constitution belge
Verjans, Pierre ULg

in Agenda Interculturel (1995), (139), 19-24

Detailed reference viewed: 28 (1 ULg)
Peer Reviewed
See detailPlasma Membrane-Dependent Activation of Gelatinase a in Human Vascular Endothelial Cells
Lewalle, J. M.; Munaut, Carine ULg; Pichot, B. et al

in Journal of Cellular Physiology (1995), 165(3), 475-83

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the ... [more ▼]

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the matrix metalloproteinase family, degrades BM type IV collagen and is involved in the BM breakdown by migrating tumor cells and endothelial cells (EC). Gelatinase A is synthesized as latent proenzyme and must be activated in order to express its proteolytic activity. A plasma membrane-dependent mechanism of activation has been described for several tumor and transformed cells lines. In the present study, we show that latent (72 kD) and mature (62-59 kD) forms of gelatinase A are present in EC membrane fraction from Triton X-114 extract while only latent form is found in the cytosolic fraction. The incubation of EC membrane fraction with exogenous latent gelatinase A resulted in a significant activation giving rise to 62-59 kD mature forms. 12-O-tetradecanoylphorbol-13-acetate (TPA), a strong potentiator of angiogenesis in vitro and in vivo, increases the amount of both latent and activated forms of gelatinase A in EC membrane fraction as well as the ability of this latter fraction to activate exogenous latent gelatinase A. We show that the mRNA transcript coding for the membrane-integrated MMP, the MT-MMP, previously described as a potential gelatinase A activator in invasive tumor cells is also expressed in vascular EC and is regulated through a TPA sensitive process. This enzyme may be responsible for membrane-dependent gelatinase A activation in normal vascular EC and may therefore be a determinant in the control of BM proteolysis during angiogenesis. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailThe use of styrenic copolymers to generate polyimide nanofoams
Hedrick, James; Hawker, Craig J; DiPietro, R. et al

in Polymer (1995), 36(25), 4855-4866

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block ... [more ▼]

New routes for the synthesis of high T-g thermally stable polymer foams with pore sizes in the nanometre regime have been developed. Foams were prepared by casting well-defined microphase-separated block copolymers comprising a thermally stable block and a thermally labile material. At properly designed volume fractions, the morphology provides a matrix of the thermally stable material with the thermally labile material as the dispersed phase. Upon thermal treatment, the thermally unstable block undergoes thermolysis generating pores, the size and shape of which are dictated by the initial copolymer morphology. Several labile blocks were surveyed including polystyrene, poly(alpha-methylstyrene) and several alpha-methylstyrene/styrene copolymers. Each of these polymers can unzip to its monomer upon heating; however, the rate is substantially slower for polystyrene. The copolymers were synthesized through either the poly(amic acid) precursor, followed by chemical imidization to the polyimide form, or the poly(amic alkyl ester) precursor followed by thermal imidization. The decomposition of the labile coblock was studied by thermogravimetric and dynamic mechanical analysis. Upon decomposition, the foams showed pore sizes in the nanometre regime along with the expected reduction in mass density. [less ▲]

Detailed reference viewed: 35 (4 ULg)
Full Text
See detailLa passion du philosophe
Durand, Pascal ULg

in Avancées (1995)

Detailed reference viewed: 29 (11 ULg)
Peer Reviewed
See detailIn vitro drug sensitivity and clinical aspects of Plasmodium falciparum malaria in African children
Carme, Bernard; Gay, Frederic; Ndounga, Mathieu et al

in Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (1995), 46(4), 270-274

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1 ... [more ▼]

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1: 39 asymptomatic school children, Group 2: 16 children with uncomplicated malaria, Group 3: 40 with severe but not pernicious malaria and Group 4: 20 with pernicious malaria. The drugs tested were chloroquine (CQ), quinine (QN) and mefloquine (MQ). The sensitivity level was assessed by a 48-hour in vitro maturation test involving the uptake of tritiated hypoxanthine, the initial blood level of parasite being > or = 0.1% in all cases. For QN and MQ, the median IC50 values showed no significant difference related to clinical status, age or parasitaemia levels. For CQ, the proportion of resistant strains and the 50 inhibitory concentration (IC50) values were greater in the cases of children hospitalised for malaria but there were no differences related to clinical severity of these hospitalised children nor, within each group, to the age or parasitaemia levels. The percentage of subjects with an IC50 value greater than the 90 percentile of the IC50 of the asymptomatic group, which we propose as the severity index related to chemoresistance, was 15% for uncomplicated malaria, 38% for severe but non-pernicious forms and 35% for pernicious malaria. The IC50 for QN was significantly higher in CQ-resistant strains and there was a positive correlation for CQ vs QN and for QN vs MQ. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Peer Reviewed
See detailDiscussions about the Bp-d diagrams in polynomial for marine propeller series
Marchal, Jean ULg; Shen, Yide ULg; Kicheva, D.

in RINA (1995), volume 137

Detailed reference viewed: 39 (1 ULg)
Full Text
Peer Reviewed
See detailTolerance to Osmotic Shocks in Rats Kidney Cortex and Medulla
Gilles, Raymond ULg; Compère, Philippe ULg; el Goumzili, M. et al

in Tissue & Cell (1995), 27(6), 667-77

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic ... [more ▼]

Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic shocks induces dramatic modifications in chromatin organization. The present paper reports on the changes of medulla cell chromatin in situ, in rat kidney slices submitted to osmotic challenges and in vitro, on preparations of extracted chromatin submitted to changes in environmental ion concentrations. Our results show that the chromatin of kidney medulla cells: (1) does not behave differently from the other mammalian chromatins when submitted in situ or in vitro to osmotic challenges; (2) presents in vitro physico-chemical characteristics similar to those of the other mammalian chromatins; and (3) is protected in vitro, as the other mammalian chromatins, from the disrupting effects of increases in inorganic ion concentrations by different compensatory organic solutes. The ability of kidney medulla cells to adapt to large increases in osmolarity could thus be related to a rapid control of the level of such compounds rather than to some rather specific, intrinsic molecular adaptations of macromolecules. [less ▲]

Detailed reference viewed: 23 (1 ULg)
Full Text
Peer Reviewed
See detailThe Beta-Lactamase Secreted by the Antarctic Psychrophile Psychrobacter Immobilis A8
Feller, Georges ULg; Sonnet, P.; Gerday, Charles ULg

in Applied and Environmental Microbiology (1995), 61(12), 4474-6

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal ... [more ▼]

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases. [less ▲]

Detailed reference viewed: 16 (0 ULg)
See detailCécité et fantastique dans rapport sur les aveugles d'Alberto Breccia
Denis, Benoît ULg; Ghizzardi, Yves

in Voir (1995), (11), 52-63

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailComment évaluer la sécrétion insulinique en pratique?
Scheen, A. J.; Paquot, Nicolas ULg; Letiexhe, M. R. et al

in Diabète & Métabolisme (1995), 21(6), 458-64

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailBeef production in Belgium: to which extent manage extensification
Istasse, Louis ULg; Clinquart, Antoine ULg; Dufrasne, Isabelle ULg et al

in Proceeding of a workshop held in Paris on Novembre 22, 23 and 24, 1995 (1995)

Detailed reference viewed: 18 (2 ULg)
Full Text
Peer Reviewed
See detailMutations Affecting the Mitochondrial Genes Encoding the Cytochrome Oxidase Subunit I and Apocytochrome B of Chlamydomonas Reinhardtii
Colin, Martine ULg; Dorthu, M. P.; Duby, Franceline ULg et al

in Molecular & General Genetics [=MGG] (1995), 249(2), 179-84

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase ... [more ▼]

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase pathway is still active the mutants have lost the capacity to grow heterotrophically (dark + acetate) and display reduced growth under mixotrophic conditions (light + acetate). In crosses between wild-type and mutant cells, the meiotic progeny only inherit the character transmitted by the mt- parent, which indicates that the mutations are located in the 15.8 kb linear mitochondrial genome. Two new mutants (dum-18 and dum-19) have now been isolated and characterized genetically, biochemically and at the molecular level. In addition, two previously isolated mutants (dum-11 and dum-15) were characterized in more detail. dum-11 contains two types of deleted mitochondrial DNA molecules: 15.1 kb monomers lacking the subterminal part of the genome, downstream of codon 147 of the apocytochrome b (COB) gene, and dimers resulting from head-to-head fusion of asymmetrically deleted monomers (15.1 and 9.5 kb DNA molecules, respectively). As in the wild type, the three other mutants contain only 15.8 kb mitochondrial DNA molecules. dum-15 is mutated at codon 140 of the COB gene, a serine (TCT) being changed into a tyrosine (TAC). dum-18 and dum-19 both inactivate cytochrome c oxidase, as a result of frameshift mutations (addition or deletion of 1 bp) at codons 145 and 152, respectively, of the COX1 gene encoding subunit I of cytochrome c oxidase. In a total of ten respiratory deficient mitochondrial mutants characterized thus far, only mutations located in COB or COX1 have been isolated.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 31 (4 ULg)
Peer Reviewed
See detailGenetic Mapping of Mitochondrial Markers by Recombinational Analysis in Chlamydomonas Reinhardtii
Remacle, Claire ULg; Colin, Martine ULg; Matagne, René-Fernand ULg

in Molecular & General Genetics [=MGG] (1995), 249(2), 185-90

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type ... [more ▼]

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type minus (paternal) parent. In contrast mitotic zygotes transmit maternal and paternal mitochondrial DNA copies to their diploid progeny and recombinational events between molecules of both origins frequently occur. Six mitochondrial mutants unable to grow in the dark (dk- mutants) were crossed in various combinations and the percentages of wild-type dk+ recombinants were determined in mitotic zygotes when all progeny cells had become homoplasmic for the mitochondrial genome. In crosses between strains mutated in the COB (apocytochrome b) gene and strains mutated in the COX1 (subunit 1 of cytochrome oxidase) gene, the frequency of recombination was 13.7% (+/- 3.2%). The corresponding physical distance between the mutation sites was 4.3 kb. In crosses between strains carrying mutations separated by about 20 bp, a recombinational frequency of 0.04% (+/- 0.02%) was found. Two other mutants not yet characterized at the molecular level were also used for recombinational studies. From these data, a linear genetic map of the mitochondrial genome could be drawn. This map is consistent with the positions of the mutation sites on the mitochondrial DNA molecule and thereby validates the method used to generate the map. The frequency of recombination per physical distance unit (3.2% +/- 0.7% per kilobase) is compared with those obtained for other organellar genomes in yeasts and Chlamydomonas. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailMacromolecular engineering of polylactones and polylactides. 20. Effect of monomer, solvent, and initiator on the ring-opening polymerization as initiated with aluminum alkoxides
Ropson, Nathalie; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(23), 7589-7598

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization ... [more ▼]

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization initiated by aluminum alkoxides. In toluene, aluminum triisopropoxide, a commonly used initiator, forms tetrameric (A(4)) and/or trimeric (A(3)) aggregates, which are dissociated upon the addition of the cyclic monomer. Actually, LA is responsible for the complete deaggregation of both A(3) and A(4) into single species (A(1)) trisolvated by the monomer (Al coordination number, CN = 6), whereas A(3) is selectively deaggregated by epsilon-CL. This behavior is consistent with the higher stability of A(4) compared to A(3). Whatever the monomer, three chains are initiated by the active A(1) species. When conversion of epsilon-CL is complete, the active aluminum trialkoxide sites are solvated by one (CN = 4) and more probably three (CN = 6) carbonyl groups of the attached poly-epsilon-caprolactone chains. At the complete LA conversion, only one carbonyl group of the polylactide chains is coordinated to Al (CN = 4). In THF, there is a competition between monomer and solvent for coordination to Al, which explains why polymerization is slower in THF than in toluene. In both toluene and THF, diethylaluminum monoalkoxide forms cyclic trimers that are solvated by the monomer (epsilon-CL) at least on the NMR time scale. Nevertheless, a fractional kinetic order in initiator is observed which gives credit to a fast reversible dissociation of the epsilon-CL-solvated trimers. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailPhase-shifting real time interferometry that uses bismut silicon oxide crystals
Georges, Marc ULg; Lemaire, Philippe

in Applied Optics (1995), 34(32), 7497-7506

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with ... [more ▼]

A bismuth silicon oxide crystal is used in the diffusion regime as a dynamic recording medium in a real-time holographic interferometer based on anisotropic self-diffraction. This device is connected with an interferogram-analysis method that uses the phase-shifting technique for quantitative measurement of diffusive-reflecting object deformations. In addition to the usual error sources in phase shifting, the temporal interferogram erasure is studied and is found weakly perturbative for the measured phase. Itis shown that quantitative measurements are possible for low-intensity object beams (8 μW/cm2) and a large observed area. Apractical situation of defect monitoring in a composite structure is presented. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailThe Replication in Vitro of the Gammaherpesvirus Bovine Herpesvirus 4 Is Restricted by Its DNA Synthesis Dependence on the S Phase of the Cell Cycle
Vanderplasschen, Alain ULg; Goltz, M.; Lyaku, J. et al

in Virology (1995), 213(2), 328-40

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to ... [more ▼]

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to determine the relationship between BHV-4 infection and the cell cycle. The temporal expression of BHV-4 late (L) proteins in unsynchronized cell cultures was first investigated by flow cytometry. Interestingly, L protein expression occurred in a limited number of cells infected with a high multiplicity of infection, and a reciprocal correlation between the percentage of positive cells and the cell density at the time of infection was demonstrated. Moreover, the finding that a BHV-4 early-late protein was expressed in nearly all the cells suggested that a blockage in the viral replication cycle occurred in some infected cells at the stage of viral DNA synthesis or L protein expression. Because this blockage could be the consequence of the dependence of one or both of these events on the cell cycle, they were investigated after infection of synchronized cell cultures. The following findings were made. (i) Cell transition through the S phase quantitatively increased the rate of BHV-4 DNA replication. (ii) BHV-4 DNA synthesis could not be detected in cells arrested in G0. (iii) Synchronization of MDBK cells with Lovastatin before infection increased the percentage of cells expressing L proteins. (iv) In contrast, infection of cells arrested in G0 led to few positive cells. Taken together these results showed that BHV-4 DNA replication and consequently the expression of L proteins are dependent on the S phase of the cell cycle. This dependence could be of importance for several biological properties of BHV-4 infection in vitro and might have implications for the biology of the virus in vivo. [less ▲]

Detailed reference viewed: 18 (4 ULg)
Peer Reviewed
See detailHighly-Expressed P100/P52 (Nfkb2) Sequesters Other Nf-Kappa B-Related Proteins in the Cytoplasm of Human Breast Cancer Cells
Dejardin, Emmanuel ULg; Bonizzi, Giuseppina; Bellahcene, Akeila ULg et al

in Oncogene (1995), 11(9), 1835-41

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the ... [more ▼]

Several observations have suggested that NF-kappa B transcription factors could be involved in carcinogenesis. To investigate the possibility that members of the NF-kappa B family participate in the molecular control of the transformed phenotype, we examined the expression of these proteins in human breast cancer cell lines as well as in primary tumors. Western Immunoblots demonstrated high expression of the p52 precursor p100 (NFKB2) in several breast cancer cell lines while human mammary epithelial cells express this protein only faintly. Eighteen primary breast tumors out of 24 displayed significant expression of the p100/p52 protein. In MDA-MB-435 cells, overexpressed p100 and p52 are predominantly cytoplasmic and coimmunoprecipitation experiments demonstrated that p100 sequesters the heterodimer p50/p65 in the cytoplasm. We demonstrate that most p65 protein is complexed with p100 in these cells while it is complexed predominantly with I kappa B-alpha in cell lines expressing less p100. Our data strengthen the hypothesis that NF-kappa B could be involved in carcinogenesis and suggest that the p100/p52 NF-kappa B subunit could play a role in the development of human breast cancers, possibly by sequestering other NF-kappa B-related proteins in the cytoplasm. [less ▲]

Detailed reference viewed: 52 (4 ULg)
Full Text
Peer Reviewed
See detailPhotometric monitoring (1987 to 1994) of the gravitational lens candidate UM 425.
Courbin, F.; Magain, Pierre ULg; Remy, M. et al

in Astronomy and Astrophysics (1995), 303

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged ... [more ▼]

We present the results of a 7 year long photometric monitoring of two components (A and B) of UM 425, thought to be images, separated by 6.5", of the same z=1.47 quasar. These components have been imaged through an R filter in order to obtain their light curves. The photometry was obtained by simultaneously fitting a stellar two-dimensional profile on each component. The brightest image (component A, m_R_=15.7) shows a slow and smooth increase in brightness of 0.2 magnitude in seven years, while the faintest one (component B, m_R_=20.1) displays an outburst of 0.4 magnitude which lasts approximately two years. The variation of component B may be interpreted in two ways, assuming UM 425 is gravitationally lensed. If it is due to an intrinsic variation of the quasar, we derive a lower limit of 3 years on the time delay from the fact that it is not observed in component A. On the other hand, if it is a microlensing "High Amplification Event", we estimate the size of the source to be ~10^-3^pc, in agreement with standard models of AGNs. These observations are consistent with the gravitational lens interpretation of the object. Furthermore, all the CCD frames obtained under the best seeing conditions have been co-added, in an attempt to detect the deflector. The final R image reveals a rich field of faint galaxies in the magnitude range m_R_~22-24. No obvious deflector, nor any system of arcs or arclets is detected, down to a limiting magnitude of m_R_~24. [less ▲]

Detailed reference viewed: 15 (3 ULg)
Full Text
Peer Reviewed
See detailStellar calibration of CCD flat fielding.
Manfroid, Jean ULg

in Astronomy and Astrophysics. Supplement Series (1995), 113

The role of star observations is advocated in the photometric calibration of the low spatial-frequency non-uniformities of CCD detectors.

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailEffect of naloxone and metoclopropamide on LH secretion in a hyperprolactinemic, hypogonadotropic, postmenopausal woman, fertility and sterility.
Verhelst, J.; Beckers, Albert ULg; Abs, R.

in Fertility and Sterility (1995), 64(5), 969-971

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year ... [more ▼]

OBJECTIVE: To determine the role of opioidergic and dopaminergic activity in the suppression of GnRH0LH in a hyperprolactinemic state. DESIGN: Case report. SETTING: University hospital. PATIENT: A 68-year-old woman with a macroprolactinoma. INTERVENTIONS: Serial 10-hour IV infusions of naloxone and metoclopramide. MAIN OUTCOME MEASURE: Serum LH concentration. RESULTS: Naloxone induced a small but significant rise of serum LH levels, which displayed a pulsatile pattern. By contrast, metoclopramide elicited no significant response in LH secretion. CONCLUSION: Opioidergic but not dopaminergic neurotransmission plays a direct role in the suppression of LH secondary to hyperprolactinemia. [less ▲]

Detailed reference viewed: 35 (0 ULg)
Peer Reviewed
See detailLe cas clinique du mois. Dépression majeure chez un patient diabétique
Pitchot, William ULg; Ansseau, Marc ULg

in Revue Médicale de Liège (1995), 50(11), 451-2

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailFactors influencing the immediate and late outcome of cushing-disease treated by transsphenoidal surgery : A retrospective study by the european cushings-disease survey group
Bochicchio, D.; Losa, M.; Buchfelder, M. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3114-3120

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of ... [more ▼]

Hypercortisolism attributable to hypersecretion of ACTH by a pituitary adenoma is an uncommon and progressively lethal disease. Because of its rarity, it has been difficult to collect a large series of patients in order to identify the prognostic factors influencing the outcome after transsphenoidal surgery. We conducted a multicenter, retrospective analysis of the early and late results of surgical treatment of Cushing's disease. Files of patients with Cushing's disease who underwent transsphenoidal surgery between 1975 and 1990 were collected from 25 institutions throughout Europe. Data from 668 of 716 patients were suitable for statistical analyses. Surgical mortality was 1.9%, and major morbidity occurred in 97 patients (14.5%). Clinical and biochemical remission of Cushing's disease after surgery occurred in 510 cases (76.3%). Identification of the tumor by neuroradiological imaging or at operation with histopathological corroboration was associated with remission of hypercortisolism. Recurrence of the disease occurred in 65 (12.7%) of 510 patients in remission after surgery at a mean time of 39.3 months (range 6-104 months). The distribution of the recurrences did not show any apparent plateau or cluster throughout the follow-up period. Low postoperative steroid levels, absence of cortisol response to CRH, and the need for long-term glucocorticoid substitution therapy were all associated with a high probability of long-term remission. Our study demonstrates that transsphenoidal surgery is a safe and effective treatment for patients with Cushing's disease. However, after successful surgery there is a steady increase in the percentage of recurrences, which continues with time. Patients who after operation had hypoadrenocorticism and needed long-term glucocorticoid substitution therapy had the lowest risk of relapse. [less ▲]

Detailed reference viewed: 227 (1 ULg)
Full Text
Peer Reviewed
See detailIs Sepsis a Mediator-Inhibitor Mismatch?
Lamy, Maurice ULg; Deby, Ginette ULg

in Intensive Care Medicine (1995), 21(Suppl 2), 250-7

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detail"Music alone survives”? The collapse of faith in sonnets by Geoffrey Hill and G.M. Hopkins’
Pagnoulle, Christine ULg

in Cahiers Victoriens & Edouardiens (1995), 42

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's ... [more ▼]

The present article explores the various ways in which Geoffrey Hill uses the regularity of the sonnet form to express the impossibility of faith, and how this collapse was announced in some of Hopkins's most famous celebratory sonnet 'The Windhover.' [less ▲]

Detailed reference viewed: 46 (12 ULg)
Full Text
Peer Reviewed
See detailEfficacy and Safety of Drugs for Paget's Disease of Bone
Reginster, Jean-Yves ULg; Lecart, M. P.

in BONE (1995), 17(5 Suppl), 485-488

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and ... [more ▼]

Paget's disease of bone is characterized by an anarchic bone turnover starting with excessive resorption caused by structural and functional abnormalities involving osteoclasts. Calcitonin and bisphosphonates are now considered as the main therapeutic approaches for this disease. Daily parenteral administration of calcitonin to patients with Paget's disease of bone results in a significant fall in serum alkaline phosphatase and urinary hydroxyproline levels. This treatment has also been reported to be effective in relieving clinical symptoms of the disease, mainly bone pain. The drawbacks of injectable calcitonin have stimulated interest in alternative routes of delivery. Substantial evidence of calcitonin bioavailability and bioefficacy equivalent to those of parenteral administration is currently available for only two alternative routes: nasal spray and rectal suppository. Since many results have been published showing a dramatic effect of several bisphosphonates in Paget's disease of bone, nasal and rectal calcitonin are no longer considered as the treatments of choice in this condition. A major advantage of the use of bisphosphonates over calcitonin in Paget's disease is that biochemical and histologic suppression of disease activity may persist for many years after the cessation of treatment. Oral etidronate and intravenous pamidronate have been extensively used and have provided satisfactory benefits to the patient. Since the risk/benefit ratio of alendronate does not appear to be completely positive, it is likely that the future of treatment of Paget's disease of bone will be based on the oral formulation of the new bisphosphonates, including tiludronate, risedronate or dimethyl-pamidronate. [less ▲]

Detailed reference viewed: 7 (2 ULg)
Full Text
Peer Reviewed
See detailThe prevalence of colonic polyps in acromegaly : a prospective colonoscopic and pathological study in 103 patients.
Delhougne, B.; Deneux, C.; Abs, R. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(11), 3223-3226

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results ... [more ▼]

Patients with acromegaly are reported to be at risk of developing adenomatous colonic polyps, which are considered to be preneoplastic lesions. This assumption is, however, usually drawn from results obtained in rather small series of patients or without a control group. We, therefore, undertook a prospective colonoscopic and pathological study comprising 103 acromegalic patients and 138 nonacromegalic control subjects referred for irritable bowel syndrome. The prevalence of adenomatous colonic polyps was significantly increased in acromegalic patients compared to that in control subjects (22.3% vs. 8.0%; P = 0.0024). The significance was similarly present in male acromegalic patients (28.6% vs. 5.5% in male control subjects; P = 0.0026), but was absent in female acromegalic patients. The prevalence of colonic polyps was also significantly increased in the group of acromegalic patients under 55 yr of age (20.0% vs. 3.0% in the control group of the same age; P = 0.0026). Other characteristics of adenomatous colonic polyps in acromegaly were the multiplicity and the presence proximal to the splenic flexure. No difference in the duration of acromegaly was found between patients with or without adenomatous polyps. The prevalence of hyperplastic colonic polyps was also significantly increased to 24.3% in acromegalic patients vs 4.4% in control subjects (P < 0.001). In conclusion, in view of the increased incidence of adenomatous colonic polyps, colonoscopy should be part of the follow-up examination in acromegaly. [less ▲]

Detailed reference viewed: 63 (6 ULg)
Full Text
Peer Reviewed
See detailPreparation of a Macroporous Biodegradable Polylactide Implant for Neuronal Transplantation
Schugens, C.; Grandfils, Christian ULg; Jérôme, Robert ULg et al

in Journal of Biomedical Materials Research (1995), 29(11), 1349-62

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an ... [more ▼]

This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an original method consisting of the aggregation of calibrated poly-D,L-lactide microparticles. The size of the interstices between the aggregated microspheres was in a direct relationship to the microsphere diameter. A first approach was based on coating the microspheres with poly(vinyl alcohol) followed by chemically crosslinking the coating layers that were in mutual contact. This method was disregarded because of the acute cytotoxicity of glutaraldehyde used as the crosslinking agent, the absence of macroporosity, and the complete lack of flexibility. A physical technique of aggregation was then tested, which relied on the plasticization of poly-D,L-lactide microspheres with triethylcitrate to the point where microspheres strongly adhered to each other when they were in contact. This method has proved to be straightforward and definitely superior to the chemical approach, particularly with respect to cytotoxicity, control of macroporosity, and flexibility. A polymer support was thus successfully which was biodegradable, macroporous( interconnected pores of 10-100 microns in diameter), and flexible. This potential medical device is presently being used for neuronal transplantation in the central nervous system. [less ▲]

Detailed reference viewed: 27 (5 ULg)
Peer Reviewed
See detailEvaluation and comparison of the behaviour at sea and forces in cables of two types of offshore tension leg platforms
Marchal, Jean ULg; Lejeune, A.

in International Symposium on CABLE DYNAMICS (1995)

Detailed reference viewed: 12 (5 ULg)
Full Text
Peer Reviewed
See detailRetinoic Acid Induces Three Newly Cloned Hoxa1 Transcripts in Mcf7 Breast Cancer Cells
Chariot, Alain ULg; Moreau, Louis; Senterre, Geoffrey et al

in Biochemical and Biophysical Research Communications (1995), 215(2), 713-20

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of ... [more ▼]

Coordinated expression of genes involved in development, differentiation and malignant transformation is regulated by transcription factors including homeodomain-containing proteins. However, most of their cDNA sequences are still unknown. We report here the molecular characterization of three newly cloned HOXA1 transcripts from human breast cancer cells. In addition, we provide evidence that these alternatively spliced transcripts encode one homeodomain-containing protein and two products lacking the conserved DNA-binding domain. Moreover, we demonstrate that all three HOXA1 transcripts are induced by retinoic acid in MCF7 cells. Taken together, our results suggest that HOXA1 gene may be a key element in the establishment of the breast cancer cell phenotype. [less ▲]

Detailed reference viewed: 17 (6 ULg)
Full Text
Peer Reviewed
See detailAnionic polymerization of pivalolactone initiated by alkali metal alkoxides
Jedlinski, Zbigniew; Kurcok, Piotr; Kowalczuk, Marek et al

in Macromolecules (1995), 28(21), 7276-7280

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the ... [more ▼]

Polymerization of pivalolactone (alpha,alpha-dimethyl-beta-propiolactone) initiated with alkali metal alkoxides has been studied. It has been revealed that after addition of an alkoxide anion, from the initiator, onto the carbonyl carbon atom of the monomer the selective cleavage of the acyl-oxygen bond of the monomer leads to the formation of alkoxide propagating species. Thus, it has been demonstrated that, in contrast to alpha-unsubstituted beta-lactones and higher lactones, the anionic polymerization of pivalolactone proceeds through either alkoxide or carboxylate propagation centers, depending on the nature of initiator used, i.e. an alkoxide or a carboxylate, respectively. In the former case, however, cyclic oligomers are also formed which are unusual in the anionic polymerization of other beta-lactones. This indicates that, whatever the lactone ring size (four-, six-, or seven-membered lactones), intramolecular transesterification reactions can take place if alcoholate ions are propagating species. [less ▲]

Detailed reference viewed: 28 (1 ULg)