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See detailBrain Aromatase and the Control of Male Sexual Behavior
Balthazart, Jacques ULg; Foidart, Agnès ULg

in Journal of Steroid Biochemistry & Molecular Biology (1993), 44(4-6), 521-40

The activational effects of testosterone (T) on male copulatory behavior are mediated by its aromatization into estradiol. In quail, we have shown by stereotaxic implantation of steroids and metabolism ... [more ▼]

The activational effects of testosterone (T) on male copulatory behavior are mediated by its aromatization into estradiol. In quail, we have shown by stereotaxic implantation of steroids and metabolism inhibitors and by electrolytic lesions that the action of T and its aromatization take place in the sexually dimorphic medial preoptic nucleus (POM). The distribution and regulation of brain aromatase was studied in this species by product-formation assays measuring aromatase activity (AA) in microdissected brain regions and by immunocytochemistry (ICC). Aromatase-immunoreactive (ARO-ir) neurons were found in four brain regions: the POM, the septal region, the bed nucleus of the stria terminals (BNST) and the tuberal hypothalamus. ARO-ir cells actually outline the POM boundaries. ARO-ir material is found not only in the perikarya of neurons but also in the full extension of their cellular processes including the axons and the presynaptic boutons. This is confirmed at the light level by the demonstration of immunoreactive fibers and punctate structures in brain regions that are sometimes fairly distant from the closest ARO-ir cells. A lot of ARO-ir cells in the POM and BNST do not contain immunoreactive estrogen receptors (ER-ir) as demonstrated by double label ICC. These morphological data suggest an unorthodox role for the enzyme or the locally formed estrogens. In parallel with copulatory behavior, the preoptic AA decreases after castration and is restored by T to levels seen in sexually mature males. This probably reflects a change in enzyme concentration rather than a modulation of the activity in a constant number of molecules since the maximum enzymatic velocity (Vmax) only is affected while the affinity (Km) remains unchanged. In addition, T increases the number of ARO-ir neurons in POM and other brain areas suggesting that the concentration of the antigen is actually increased. This probably involves the direct activation of aromatase transcription as demonstrated by RT-PCR studies showing that aromatase mRNA is increased following T treatment of castrates. These activating effects of T seem to result from a synergistic action of androgenic and estrogenic metabolites of the steroid. The anatomical substrate for these regulations remains unclear at present especially in POM where ARO-ir cells do not in general contain ER-ir while androgen receptors appear to be rare based on both [3H] dihydrotestosterone autoradiography and ICC. Transynaptic mechanisms of control may be considered. A modulation of brain aromatase by catecholamines is also suggested by a few pharmacological studies.(ABSTRACT TRUNCATED AT 400 WORDS) [less ▲]

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See detailSequential anti-core glycolipid immunoglobulin antibody activities in patients with and without septic shock and their relation to outcome
Nys, Monique ULg; Damas, Pierre ULg; Joassin, Luc et al

in Annals of Surgery (1993), 217(3), 300-306

OBJECTIVE: This study follows the sequential changes in anti-lipopolysaccharide antibodies in infected patients with and without septic shock. SUMMARY BACKGROUND DATA: A relation between high endogenous ... [more ▼]

OBJECTIVE: This study follows the sequential changes in anti-lipopolysaccharide antibodies in infected patients with and without septic shock. SUMMARY BACKGROUND DATA: A relation between high endogenous levels of anti-LPS antibodies and protection against bacteremia and septic shock in at-risk patient groups has been observed. However, information on the daily follow-up and kinetics of apparition or disappearance of anti-LPS antibody activities and their relations with the protective properties of the different immunoglobulin classes has not been clearly investigated. METHODS: Two hundred and five septic surgical patients were studied during their stay in the intensive care unit during a period of 3 years. Among these patients, septic shock developed in 54 and 47 died. A sensitive ELISA was used to study circulating IgM and IgG antibodies to the core glycolipid (CGL) region of Salmonella minnesota R595. The activities were measured each day when sepsis occurred and every hour during septic shock. RESULTS: Anti-CGL IgM activity was found in 32% of the septic patients. This response, however, most often appeared to be transient. A strong correlation was observed between the occurrence of septic shock and the absence of anti-CGL IgM activity on admission to the ICU (p < 0.02). Anti-CGL IgG activity was detected in 82% of the patients and better correlated with outcome for patients with high or rising activities during their hospitalization (p < 0.0005). In patients with septic shock or irreversible organ failure, a fall in the anti-CGL IgG activity was observed before death, suggesting that the IgG antibodies were consumed during this acute event. Therefore, the anti-CGL IgG activity measured by ELISA could be used as a marker of the evolution of the illness. CONCLUSIONS: Our observations demonstrate the interest to follow-up the evolution of the anti-CGL antibodies during sepsis. The fall of these antibodies during septic shock and in patients who died was an additional argument to perform, as an additive form, passive antibody therapy to decrease lethality in this group of patients. [less ▲]

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See detailL'angoisse minimaliste
Demoulin, Laurent ULg

in Indications. La Revue des Romans (1993), 1(50), 27-31

Description de quelques caractéristiques du roman français contemporain.

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See detailComparative immunohistochemical study of herpes-simplex and varicella-zoster infections
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1993), 422(2), 121-126

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new ... [more ▼]

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387-and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis. [less ▲]

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See detailSobre a construção de porjetores ortogonais
Iemma, A. F.; Palm, Rodolphe ULg; Claustriaux, Jean-Jacques ULg

in Revista de Matemática e Estatìstica (1993), 11

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See detailBarium isotopes in the very metal-poor star HD 140283
Magain, Pierre ULg; Zhao, G.

in Astronomy and Astrophysics (1993), 268

The fractions of odd and even isotopes of barium in the metal-poor star HD140283 are determined from an analysis of the profile of the Ba II resonance line, taking advantage of the differences in ... [more ▼]

The fractions of odd and even isotopes of barium in the metal-poor star HD140283 are determined from an analysis of the profile of the Ba II resonance line, taking advantage of the differences in hyperfine structure splitting between the different isotopes. Although one would expect an increased contribution of the r-process in very metal-poor stars, it is found that the isotopic ratio is very close to solar. [less ▲]

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See detailMagnitudes des étoiles doubles.
Renson, Pierre ULg

in Ciel et Terre (1993), 109

Not Available

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See detailEvaluation quantitative de l'érythème par colorimétrie. Effets de l'histamine de corticostéroïdes et d'extraits végétaux
Arrese Estrada, Jorge ULg; Nikkels, Arjen ULg; El Fekih, N. et al

in Journal d'Actualités Médicales Belges (1993), 5

L'évaluation de l'érythème est difficile et souvent aléatoire en clinique. Afin d'étudier la couleur des téguments, la colorimétrie est proposée depuis quelques années. Elle permet plus de rigueur. En ... [more ▼]

L'évaluation de l'érythème est difficile et souvent aléatoire en clinique. Afin d'étudier la couleur des téguments, la colorimétrie est proposée depuis quelques années. Elle permet plus de rigueur. En tous cas, elle permet d'aligner des chiffres dont les variations peuvent être étudiées dans le temps. Le présent travail a permis de tester, de "calibrer" la méthodologie colorimétrique dans le domaine de l'érythème cutané. D'une part, l'effet vasodilatateur (érythémateux) de l'histamine, de l'autre, le blanchiment induit par l'application de topiques corticoïdes et phytopharmaceutiques ont été mesurés et discutés. [less ▲]

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See detailAspects immunologiques et hématologiques de l'infection par le virus HIV
Delhougne, B.; Fillet, Georges ULg

in Revue Médicale de Liège (1993), 48(2), 72-80

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See detailLas cosas escritas en la Nueva España
Vanden Berghe, Kristine ULg

in L'ordinaire. Mexique Amérique Centrale (1993), 143

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See detailStrychnopentamine, a Potential Anticancer Agent
Quetin-Leclercq, J.; Bouzahzah, B.; Pons, A. et al

in Planta Medica (1993), 59(1), 59-62

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg ... [more ▼]

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg strychnopentamine (1 per day) induce a significant decrease of the number of tumor cells and a significant increase of the survival of the treated mice. Observed side effects are partial haemolysis and some liver damage. [less ▲]

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See detailEfficient Immunoselection of Cytolytic Effectors with a Magnetic Cell Sorter
Jacobs, Nathalie ULg; Moutschen, Michel P; Boniver, Jacques ULg et al

in Research in Immunology (1993), 144(2), 141-50

This paper describes a rapid and efficient method for the sorting of in vitro activated cytolytic effectors cells. For cytotoxic assays, a large number of cells with conserved function must be rapidly ... [more ▼]

This paper describes a rapid and efficient method for the sorting of in vitro activated cytolytic effectors cells. For cytotoxic assays, a large number of cells with conserved function must be rapidly obtained. Immunomagnetic sorting was chosen because it is faster than flow cytometry sorting. The MACS system requires the use of paramagnetic beads of small diameter (100-150 nm), reputed to interfere minimally with cell function. In order to generate the cytolytic effectors, peripheral blood lymphocytes were cultivated in the presence of interleukin-2 (50 U/ml) and anti-CD3 monoclonal antibody (BMA030, 100 ng/ml) for 4 days. Cell separation was based on the membrane expression of the CD3 complex. The purity obtained for positive (CD3+) cell sorting with the MACS was higher than 95%. The purity of negative (CD3-) cell fraction was more variable, but further purification by flow cytometry rapidly yielded purity higher than 95%. Cytotoxic assays were performed against four target cell lines (K562, Daudi, HL60 and U937) and proliferation assays showed that both negatively and positively selected populations had conserved their function acquired during culture in the presence of anti-CD3 mAb and IL2. [less ▲]

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See detailMultiple System Organ Failure after open-heart surgery in infants and children
Seghaye, Marie-Christine ULg; Engelhardt, W.; Grabitz, R. G. et al

in Thoracic and Cardiovascular Surgeon (1993), 41(1), 49-53

Between January 1985 and March 1989 we retrospectively observed Multiple System Organ Failure (MSOF) in 16 of 460 children (3.5%) who underwent cardiac surgery with cardiopulmonary bypass for congenital ... [more ▼]

Between January 1985 and March 1989 we retrospectively observed Multiple System Organ Failure (MSOF) in 16 of 460 children (3.5%) who underwent cardiac surgery with cardiopulmonary bypass for congenital heart disease. MSOF was arbitrarily defined as a clinical entity with failure of two or more vital organ systems associated with high fever, thrombocytopenia, and cardiocirculatory insufficiency and occurring within the first postoperative week. In 13 children the first clinical manifestations of MSOF were evident on the first postoperative day and in the other 3 on the second or third postoperative day. All children showed acute renal failure, acute hepatic failure, high fever, and thrombocytopenia. Most of them showed respiratory insufficiency and neurological involvement. Seven of the 16 children died. Four of the 9 surviving patients had neurological sequelae still present 6 months after the operation, and the others recovered completely. [less ▲]

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See detailAn Unusual Case of Cor Triatriatum: Clinical Presentation and the Haemodynamics of a Rare Anomaly
Radermecker, M. A.; Grenade, Thierry ULg; Jalali, H. et al

in Cardiovascular Surgery (1993), 1(1), 83-5

The case of a 24-year-old woman with cor triatriatum sinister and a persistent left superior vena cava connected to the junction of the left upper pulmonary vein and left atrium is reported. A diagnosis ... [more ▼]

The case of a 24-year-old woman with cor triatriatum sinister and a persistent left superior vena cava connected to the junction of the left upper pulmonary vein and left atrium is reported. A diagnosis of partial anomalous pulmonary venous return had been made and the patient underwent surgery. Inspection of the left atrium revealed a complex form of left triatrial heart. The general haemodynamics of the malformation are discussed, with particular emphasis on the features accounting for variation in the clinical presentation. This rare anomaly often remains undiagnosed before surgery and should be considered whenever obstruction of pulmonary venous flow above the mitral valve is not explained by pulmonary vein stenosis, mitral or mitral valve-linked abnormalities, atrial tumours or anomalous pulmonary venous drainage with high resistance to flow. [less ▲]

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See detailBrachymorphism-Onychodysplasia-Dysphalangism Syndrome
Verloes, Alain ULg; Bonneau, D.; Guidi, O. et al

in Journal of Medical Genetics (1993), 30(2), 158-61

Three unrelated children are reported with intrauterine proportionate growth retardation and facial dysmorphism (broad nose, flat malar area, large mouth, pointed chin), microcephaly, hypo/aplasia of the ... [more ▼]

Three unrelated children are reported with intrauterine proportionate growth retardation and facial dysmorphism (broad nose, flat malar area, large mouth, pointed chin), microcephaly, hypo/aplasia of the terminal fifth digits, and (sub)normal intelligence. Radiological findings include hypo/aplasia or fusion of the distal phalanges of the fifth finger and toe, brachymesophalangism V, and nail dysplasia or aplasia. One child had cystic adenomatoid disease of the lung. The pattern of anomalies presented by these children closely resembles a syndrome incompletely delineated in 1971 by Senior in six children, which has often been considered to be a mild form of Coffin-Siris syndrome. We suggest that this is an independent entity (BOD syndrome). The aetiology is still unknown. Differential diagnosis and nosological difficulties are discussed. [less ▲]

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See detailCloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10
Arpigny, J. L.; Feller, Georges ULg; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1993), 1171(3), 331-3

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M ... [more ▼]

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold. [less ▲]

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See detailAutres médiateurs de l'inflammation et du sepsis
Lamy, Maurice ULg; Deby, Ginette ULg; Damas, Pierre ULg

in Revue du Praticien (La) (1993), 43(1), 19-25

During sepsis, the systemic inflammatory response is characterized by the release of numerous mediators supporting and dispersing inflammation. In Gram negative sepsis, the endotoxins play a starting role ... [more ▼]

During sepsis, the systemic inflammatory response is characterized by the release of numerous mediators supporting and dispersing inflammation. In Gram negative sepsis, the endotoxins play a starting role, while in other sepsis, the triggering mediators or mechanisms are unknown but lead to a similar inflammatory reaction. Coagulation and complement cascades are activated, with the release of chemoattractive substances, mediators and proteases and the activation of phagocytic cells. Macrophages/monocytes and polymorphonuclear leucocytes produce then active oxygen species and cytokines; they degranulate (releasing active enzymes such as myeloperoxidase), they express an increasing number of membrane receptors able to interact with endothelial cells and release a supplementary lot of inflammatory mediators (prostanoids, platelet activating factor, leukotrienes ... ). Platelets, also activated, produce the same mediators (TXA2, PAF ...) or specific ones such as serotonine, platelet factor 4, growth factors. Last, but not least, the endothelial cells are stimulated, directly (by endotoxins) or undirectly (by cytokines, C5a, PAF ...). These cells play then a main role by their own phagocytic activity, by alteration of their antithrombotic and fibrinolytic potential, by their secretion of inflammatory mediators and by an increased expression of receptors of adhesivity for the activated phagocytes or platelets, what leads to endothelium injury with membrane permeability alterations. These cascades of activation, these extensions of the inflammatory reaction by the mediators and by the phagocytes and platelets can explain the frequency of multiple organ failure during sepsis as well as the difficulty of an adequate pharmacological therapy. [less ▲]

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See detailA first look at the ASSI ultraviolet results
Chakrabarti, S.; Gladstone, G. R.; Tobiska, W. K. et al

in Advances in Space Research (1993), 13

The Airglow and Solar Spectrometer Instrument (ASSI) on the San Marco D satellite has obtained near-simultaneous measurements of solar irradiances and airglow emissions in the 200-7000 A spectral region ... [more ▼]

The Airglow and Solar Spectrometer Instrument (ASSI) on the San Marco D satellite has obtained near-simultaneous measurements of solar irradiances and airglow emissions in the 200-7000 A spectral region. The satellite was placed in an equatorial, elliptical orbit on 25 March 1988, which permitted observations of airglow emissions in the 280-600-km altitude range at various local times. The instrument complement on the satellite provides an opportunity both for self-consistent examination of the excitation mechanisms of various airglow features and for constraining model parameters. An overview of the data obtained by ASSI is presented along with preliminary modeling results of the UV airglow. [less ▲]

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See detailVivre avec un nouveau coeur
Triffaux, Jean-Marc ULg

in Revue Médicale de Liège (1993), 48(1), 19-25

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See detailMisdiagnosed Malignancy in Transplanted Organs
Detry, Olivier ULg; Detroz, Bernard ULg; D'Silva, M. et al

in Transplant International (1993), 6(1), 50-4

The case reports of three patients who received cancer-bearing organs at this institution are presented. A fourth recipient, who was to be transplanted with a cancerous kidney, was spared this disastrous ... [more ▼]

The case reports of three patients who received cancer-bearing organs at this institution are presented. A fourth recipient, who was to be transplanted with a cancerous kidney, was spared this disastrous complication. The relevant data regarding the donors is also alluded to, with special reference to the type and site of the primary malignancy. Following these case reports, the implications of these issues, their possible prevention, and further management are discussed. [less ▲]

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See detailPhotophysical investigation of Rose Bengal in aqueous sodium taurocholate solutions
Seret, Alain ULg; Van de Vorst, Albert

in Journal of Photochemistry and Photobiology B : Biology (1993), 17(1), 47-56

The photophysics of rose bengal has been studied in aqueous sodium taurocholate solutions with or without 0.6 mol NaCl dm−3. The ground and first excited singlet states solubilize only in the aggregates ... [more ▼]

The photophysics of rose bengal has been studied in aqueous sodium taurocholate solutions with or without 0.6 mol NaCl dm−3. The ground and first excited singlet states solubilize only in the aggregates formed by sodium taurocholate, whereas the first triplet state solubilizes both in the bulk phase and in the aggregates. A lower limit of the corresponding entry and exit rate constants ke and ks, respectively has been estimated: ke ≥ 5 X 106 dm3 mol−1 s−1 (2x107 dm3 mol−1 s−1 with 0.6 mol NaCl dm−3) and ks ≥ 105 s−1. The triplet state as well as the semioxidized radical react with the taurocholate anion. The former reaction leads to the formation of semireduced dye. The latter is responsible for the detection of only semioxidized radicals which move freely in the aqueous phase. The results are compared with those obtained previously in aqueous sodium dodecyl sulphate solutions and the possible biological implication is discussed. [less ▲]

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See detailPlasma Na-K ATPase inhibitor activity and intracellular ions during hemodialysis
Krzesinski, Jean-Marie ULg; Du, F.; Pequeux, M. L. et al

in International Journal of Artificial Organs (1993), 16(1), 23-30

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic ... [more ▼]

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic controls (NC). As compared with the NC population, significantly enhanced values for erythrocyte (RBC) Na, Ca, platelet cytosolic Ca and EDLS were observed in all the uremic patients tested just before a dialysis session, as well as a decrease in RBC Ca ATPase and in the platelet pH. In uremia, significant correlations have been noted between RBC Na and platelet Ca (r = 0.6) or systolic BP (r = 0.45); between platelet Ca and systolic blood pressure (r = 0.8) or diastolic BP (r = 0.5) and between EDLS and RBC Na, Ca or platelet Ca (r = 0.5). Anti-hypertensive treatment has no influence on these parameters. During dialysis, a significant decrease has been noted in RBC Na, Ca, platelet Ca, SBP (only in untreated patients) and EDLS and an increase in RBC Ca ATPase and platelet pH. These modifications are significantly correlated with the weight change. [less ▲]

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See detailPaget's Disease of Bone Treated with a Five Day Course of Oral Tiludronate
Reginster, Jean-Yves ULg; Lecart, Marie-Paule ULg; Deroisy, Rita ULg et al

in Annals of the Rheumatic Diseases (1993), 52(1), 54-7

Chloro-4-phenyl thiomethylene bisphosphonate (tiludronate) is a new drug which can be used as an inhibitor of bone resorption. As it remains in bone for a long time, and as mineralisation defects have ... [more ▼]

Chloro-4-phenyl thiomethylene bisphosphonate (tiludronate) is a new drug which can be used as an inhibitor of bone resorption. As it remains in bone for a long time, and as mineralisation defects have only been seen at doses much higher than those required to decrease osteoclastic activity, it could be given at high doses over a short period of time. Eighteen patients with Paget's disease of bone were randomly allocated to three therapeutic groups receiving respectively 600, 800, and 1200 mg/day tiludronate for five days. Serum alkaline phosphatase activity and the urinary hydroxyproline/creatinine ratio were quickly and drastically reduced in all three groups. A significant reduction of serum alkaline phosphatases and the hydroxyproline/creatinine ratio was still present six months after the five day therapeutic course, reflecting a sustained activity of tiludronate even after stopping treatment. Dose dependent short and long term reductions of bone turnover rate were observed. Biochemical assessment of haematological, renal, or hepatic tolerance did not show any toxicity of tiludronate. Fifty per cent of patients treated by a dose of 1200 mg/day reported gastrointestinal disturbances, however, making this dosage unsuitable for clinical practice. [less ▲]

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See detailA novel mutation in the proteolipid protein gene leading to Pelizaeus-Merzbacher disease.
Otterbach, B.; Stoffel, W.; RAMAEKERS, Vincent ULg

in Biological chemistry Hoppe-Seyler (1993), 374(1), 75-83

Point mutations of the gene of human proteolipid protein (PLP) have been recognized as the molecular basis of one form of leukodystrophy, the X-chromosome-linked Pelizaeus-Merzbacher disease (PMD). We ... [more ▼]

Point mutations of the gene of human proteolipid protein (PLP) have been recognized as the molecular basis of one form of leukodystrophy, the X-chromosome-linked Pelizaeus-Merzbacher disease (PMD). We report the molecular analysis of four PMD patients in three unrelated families and describe a point mutation (G-->A transition) in exon V which leads to the substitution of Gly216 by a serine residue in a highly conserved extracytosolic domain and a Mae I RFLP. Molecular modelling with energy minimization indicates that this seemingly minor alteration of the amino-acid sequence induces a considerable conformational change and tight packing of the polypeptide chain apparently not compatible with the regular PLP function in oligodendrocytes. This mutation has been detected and characterized by PCR amplification of genomic DNA using intron and exon primers and the complete sequence analysis of the seven exons and a 300 bp promoter region of the PLP gene of two affected brothers. The sequence analysis of a PCR fragment representing exon V amplified from genomic DNA of different kindreds of the pedigree revealed the mother as the only carrier indicating that the mutation has occurred de novo in the mother's germline. PLP gene (including the 8.8 kb intron I) rearrangements have been excluded by Southern blot hybridization and overlapping PCR amplification of genomic DNA. [less ▲]

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See detailIdentification of Different Target Glycoproteins for Bovine Herpes Virus Type 1-Specific Cytotoxic T Lymphocytes Depending on the Method of in Vitro Stimulation
Denis, M.; Slaoui, M.; Keil, G. et al

in Immunology (1993), 78(1), 7-13

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from ... [more ▼]

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from immune cattle. Peripheral blood mononuclear cells (PBMC) expanded in vitro in the presence of interleukin-2 (IL-2) and lysed both gIII- and gIV-infected target cells. Secondary in vitro stimulation of PBMC was also performed in the presence of either fixed BHV-1-infected autologous fibroblasts or ultraviolet (UV)-inactivated virus. Both methods of antigen presentation allowed the proliferation of BHV-1-specific CTL but the target glycoprotein for these CTL differed depending on the method of stimulation. Vaccinia-gIV-infected targets were lysed predominantly when PBMC were stimulated by fixed infected fibroblasts, whilst PBMC stimulated by UV-inactivated virus lysed mostly vaccinia-gIII-infected targets. This observation could be explained by a different processing pathway of BHV-1 antigens in each cell type involved. [less ▲]

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See detailResults of metagnostic (system-oriented) and diagnostic (process-oriented) models
Beckers, Jean-Marie ULg; Haus, J.; Nihoul, Jacques ULg et al

in Progress in Belgian Oceanographic Research (1993)

After a review of the Mediterranean system, and the justification of the scientific interest, we examine the type of model (scope, purview and resolution) used for the description and simulation of the ... [more ▼]

After a review of the Mediterranean system, and the justification of the scientific interest, we examine the type of model (scope, purview and resolution) used for the description and simulation of the Mediterranean circulation from a system-oriented view and a process-oriented view. These two aspects of mathematical simulations are then illustrated by two examples: a) The simulation of the month to month variability of the general circulation in the Western Mediterranean Sea shows the main physical features, but the choice of initial conditions is crucial for a realistic simulation, and an inverse model is proven to be absolutely necessary for a valuable prognostic model. b) Secondly, a high resolution simulation of the Algerian Current instability is performed. It is well known that the Atlantic water flows along the Algerian coast as a light water intrusion. This current is unstable, and mesoscale activities generate cyclones and anticyclones, but only the latter grow enough to get separated from the mean flow. Numerical simulations, in an idealized case, and reality show strong instabilities that are analyzed by energy budgets. It is suggested that the instabilities are primarily baroclinic, which is consistent with initial vertical movements at the front. [less ▲]

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See detailComparison between Alprazolam and Hydroxyzine for Oral Premedication
Franssen, Colette ULg; Hans, Pol ULg; Brichant, Jean-François ULg et al

in Canadian Journal of Anaesthesia = Journal Canadien d'Anesthésie (1993), 40(1), 13-7

The safety and efficacy of alprazolam and hydroxyzine administered orally as surgical premedicants were compared in a double-blind controlled study. Sixty-five patients were given either alprazolam 1 mg ... [more ▼]

The safety and efficacy of alprazolam and hydroxyzine administered orally as surgical premedicants were compared in a double-blind controlled study. Sixty-five patients were given either alprazolam 1 mg or hydroxyzine 75 mg, one to two hours before surgery. Anxiety was assessed by both the patient and the anaesthetist, the patient using a visual analogue scale, the anaesthetist employing both analogue and ordinal ratings. Sedation was assessed by the anaesthetist only, using the same two methods. Amnesia was appraised with a simple memory test. Safety was assessed by recording adverse effects and measuring haemodynamic variables. Premedication with alprazolam produced a modest reduction in anxiety (28%) (P < 0.01) while hydroxyzine had no detectable effect. The comparison of the sedation level and of the memory test revealed no difference between the two premedicants. Minor side effects were only observed in the hydroxyzine group. Changes in blood pressure were more pronounced in the hydroxyzine group. This study shows that alprazolam and hydroxyzine are safe and efficient oral premedicants. However, alprazolam is preferable to hydroxyzine in terms of anxiolytic and adverse effects. [less ▲]

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See detailPulmonary Response to Intravenous Administration of 5-Hydroxytryptamine after Type-2 Receptor Blockade in Healthy Calves
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in American Journal of Veterinary Research (1993), 54(1), 168-73

The purpose of this study was to investigate the mechanism by which 5-hydroxytryptamine (5-HT) modifies respiratory function, specifically, hyperventilation, diffuse bronchoconstriction, and pulmonary ... [more ▼]

The purpose of this study was to investigate the mechanism by which 5-hydroxytryptamine (5-HT) modifies respiratory function, specifically, hyperventilation, diffuse bronchoconstriction, and pulmonary arterial hypertension in cattle. We determined whether the IV response to 5-HT in calves was attributable to stimulation of 5-HT2 receptors. Six healthy unsedated young bull calves of the Friesian (n = 4) and of the Belgian White and Blue (n = 2) breeds were used. A specific 5-HT2 antagonist (metrenperone, 0.05 mg/kg of body weight) was administered IM 30 minutes before the cattle were given a 5-minute IV 5-HT infusion. Pulmonary function values were registered before, during, and after the 5-HT challenge infusion. Minute volume increased significantly, because of an increase in respiratory rate. Conversely, lung dynamic compliance, total pulmonary resistance, and pulmonary arterial pressure were not changed. We concluded that in cattle, 5-HT-induced ventilatory response is not mediated through activation of 5-HT2 receptors. However, the 5-HT2 receptors are involved in 5-HT-induced broncho- and pulmonary vasoconstriction. [less ▲]

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See detailNew Immigrants and Democratic Society. Minority Integrations in Western Societies
Martiniello, Marco ULg

in International Migration Review (1993), 27(101), 204-205

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See detailAtomic data and the spectrum of the solar photosphere.
Grevesse, Nicolas ULg; Noels-Grötsch, Arlette ULg

in Physica Scripta (1993), 47

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the ... [more ▼]

Even though the solar photospheric abundances are now on the whole in very good agreement with the meteoritic values, some problems remain which will only be solved with a higher level of accuracy in the atomic data, especially the transition probabilities for faint lines. The authors justify this request for many more very accurate gf-values through a few particularly striking points. [less ▲]

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See detailDetection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by PCR in the worm nereis diversicolor
Salzet-Raveillon, B.; Rentier-Delrue, Françoise ULg; Dhainaut, A.

in Cellular and Molecular Biology (1993)

Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment (1-33th) and an 43 amino acids long internal fragment, two oligonucleotides primers were ... [more ▼]

Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment (1-33th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated. [less ▲]

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See detailA sulfonylthiourea (BM 20) related to torasemide: a new loop diuretic with relative potassium-sparing properties
Masereel, B.; Schynts, M.; Krzesinski, Jean-Marie ULg et al

in Journal of Pharmacy & Pharmacology (1993), 45

A series of sulphonylthioureas related to torasemide, a high ceiling loop diuretic, were synthesized and found to inhibit the Na+ 2Cl- K+ co-transporter of the thick ascending limb of the loop of Henlé ... [more ▼]

A series of sulphonylthioureas related to torasemide, a high ceiling loop diuretic, were synthesized and found to inhibit the Na+ 2Cl- K+ co-transporter of the thick ascending limb of the loop of Henlé. Their diuretic properties were studied (30 mg kg-1) after oral administration to rats. Lipophilic derivatives very active in-vitro, were found inactive orally and intraperitoneally in rats. The four most active compounds were examined for their dose-dependent diuresis. Three of them showed a potency, water and electrolyte excretion similar to torasemide. The fourth molecule, a sulphonylthiourea (BM 20), exhibited relative potassium-sparing properties and a minimal diuretic dose of 0.001 mg kg-1, 200 times lower than torasemide. [less ▲]

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See detailChiral theory of mesons in dense baryonic matter (II) : Meson propagation
Jaminon, Martine ULg; Mendez Galain; Stassart, Pierre ULg

in Nuclear Physics A (1993), 556

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See detailIdentification of 108k, 93k, and 42k Glycoproteins of Bovine Herpesvirus-1 by Monoclonal Antibodies
Baranowski, E.; Dubuisson, J.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 133(1-2), 97-111

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins gI, gIII, and gIV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by immunization of ... [more ▼]

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins gI, gIII, and gIV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by immunization of mice with either BHV-1 envelope or virus infected cells, from which the glycoproteins gI, gIII, and gIV were removed by immunoaffinity. In the latter immunization procedure mice were tolerized either against normal cellular antigens with or without glycoproteins gI, gIII, gIV, and nucleocapsid. From 154 anti-BHV-1 hybridomas isolated, 39 MAbs precipitated a 108K glycoprotein. Two other glycoproteins of respectively 42K and 93K were precipitated each by one MAb. These three glycoproteins were detected in infected cell lysate. Nine anti-108K glycoprotein MAbs neutralized BHV-1 infectivity and three non-neutralizing MAbs were able to reduce plaque development when virus was grown in the presence of these MAbs. It is therefore suggested that this glycoprotein is involved in viral entry into the cell and in cell-to-cell spread of the virus. [less ▲]

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See detailDire et écrire pour aller lire en géographie
Partoune, Christine ULg

in Bulletin de la Société Géographique de Liège (1993), 28

Texts, maps and the various documents that we use at school are almost all basically rational and cut off from reality. Before tackling them, it is proposed that we first delve into this "reality" by ... [more ▼]

Texts, maps and the various documents that we use at school are almost all basically rational and cut off from reality. Before tackling them, it is proposed that we first delve into this "reality" by means of fiction. Verbal expression gaines and written games enable us to explore beforehand the mental representation of pupils and provide us with interesting questions for subsequent research. Two approaches of this type are detailed here in order to propose a discussion of their interest and of their relevance. [less ▲]

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See detailLaminin Receptors and Laminin-Binding Proteins During Tumor Invasion and Metastasis
Castronovo, Vincenzo ULg

in Invasion & Metastasis (1993), 13(1), 1-30

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]

Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several controversies and confusion generated mainly by two events. First, the identification of several new laminin-binding proteins has raised the difficult task of attributing specific functions to specific receptors in contrast to initial beliefs that all cellular laminin-driven biological activities were mediated through the 67 LR. The second source of controversy is the large molecular-weight discrepancy between the 37-kD polypeptide encoded by the 67 LR cDNA clone and the mature 67 LR. In this manuscript, a critical and extensive review of the data accumulated on the 67 LR is presented regarding both its molecular structure and its role during tumor invasion and metastasis. A hypothetical model of the 67 LR is also proposed. Since the first identification of the 67 LR, at least 14 other cell surface molecules have been reported to be potential laminin receptors or laminin-binding proteins. These include members of the beta-galactoside-binding lectin family, seven members of the integrin family, the galactosyltransferase and some not yet fully characterized cell surface molecules. These laminin receptors and laminin-binding proteins are also described and their functions are also discussed with a particular emphasis on their participation in the constitution of the invasive phenotype. [less ▲]

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See detailLaparoscopic supracervical (subtotal) hysterectomy (LASH).
Donnez, Jacques; NISOLLE, Michelle ULg

in Journal of Gynecologic Surgery (1993), 9(2), 91-4

The use of laparoscopically assisted vaginal hysterectomy with or without annexectomy has been widely discussed. We report the technique of laparoscopic supracervical (subtotal) hysterectomy (LASH), which ... [more ▼]

The use of laparoscopically assisted vaginal hysterectomy with or without annexectomy has been widely discussed. We report the technique of laparoscopic supracervical (subtotal) hysterectomy (LASH), which was first performed in 1990. Laparoscopic supracervical hysterectomy was carried out in a series of 36 women. The duration time was 60 min in experienced hands. There were no major complications. The feasibility and low morbidity rate of this laparoscopic approach led us to propose LASH in certain indications, particularly in cases of a uterus with multiple submucosal myomas where hysteroscopic therapy is less successful. [less ▲]

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See detailFurther studies on the genetic diversity of lima beans (Phaseolus lunatus L.) using allozymes
Maquet, Alain; Wathelet, Bernard ULg; Baudoin, Jean-Pierre ULg

in Annual Report of the Bean Improvment Cooperative (1993), 36

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See detailMachine Intelligent Automatic Recognition of Critical Mobile Targets in Laser Radar Imagery
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

in Lincoln Laboratory Journal (1993), 6(1), 161-186

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See detailDifferent Mechanisms of Extracellular Matrix Remodeling by Fibroblasts in Response to Human Mammary Neoplastic Cells
Noël, Agnès ULg; Munaut, Carine ULg; Nusgens, Betty ULg et al

in Invasion & Metastasis (1993), 13(2), 72-81

Human breast tumors are often associated with a fibrotic reaction termed desmoplasia. Tumor cells may indirectly modulate the composition of the extracellular matrix by influencing fibroblast properties ... [more ▼]

Human breast tumors are often associated with a fibrotic reaction termed desmoplasia. Tumor cells may indirectly modulate the composition of the extracellular matrix by influencing fibroblast properties. They may also directly interact with collagen fibrils leading to retraction of the matrix. We have studied in vitro the influence of various human mammary tumor cells on the proliferation rate of normal human fibroblasts and on their level of collagen synthesis, as well as their release of collagenase activity. Interactions between neoplastic cells and collagen matrix were investigated by incorporation of tumor cells in collagen gels (lattices) and measurement of their retraction. All cells tested (HBL100, SW613, SA52, MDA-MB-231, MCF7, MCF7/6, MCF7 ras, BT20 and T47D) were able to modulate the composition of the extracellular matrix by one or several of the mechanisms investigated. Our results also demonstrate an opposite regulation of collagen and collagenase production. The effects on the collagen metabolism and on fibroblast proliferation are probably mediated by soluble cytokines since they are reproduced by incubating the fibroblasts in the presence of medium conditioned by tumor cells. The desmoplastic reaction may thus result from different mechanisms dependent upon tumor cell types. [less ▲]

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See detailSubjective shortening in human's memory for stimulus duration
Wearden, J. H.; Ferrara, André ULg

in Quarterly Journal of Experimental Psychology (1993), 46B(2), 163-186

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See detailComputed subsidence of the central area of Shanghai
Dassargues, Alain ULg; Radu, Jean-Pol ULg; Charlier, Robert ULg et al

in Bulletin of Engineering Geology & the Environment (1993), 47

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See detailThe peripheral analgesic effects of opiods
Joris, Jean ULg; Hargreaves, Kenneth M

in APS Journal (1993), 2(1), 51-59

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See detailProduction of active oxygen species by isolated human chondrocytes.
Henrotin, Yves ULg; Deby-Dupont, G.; DEBY, C. et al

in British Journal of Rheumatology (1993), 32(7), 562-7

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric ... [more ▼]

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric method (production of dichlorofluorescein from a precursor in the presence of horseradish peroxidase and H2O2) and by a chromatographic method (measurement of ethylene production from gamma-methiol-keto-butyric acid after .OH attack). Chondrocytes were tested, both with and without activation by phorbol myristate acetate (PMA: 10(-6) M), in the presence of Ca2+ (1 x 10(-4) M) and Mg2+ (2 x 10(-4) M) or after variable periods of anoxia under nitrogen (4 to 12 h) followed by reoxygenation (with 95% O2, 5% CO2). Under these experimental conditions, the PMA-excited chondrocytes produced from 80 to 180 nmol of hydrogen peroxide per 1 x 10(6) cells and chondrocytes subjected to anoxia-reoxygenation produced up to 1700 nmol H2O2 per 1 x 10(6) cells. The hydroxyl radical production by PMA or anoxia-reoxygenation excited cells reached 600% of the production of non-excited cells and 1300% when they were subjected to successive stimulations by PMA and anoxia-reoxygenation. The possible pathological significance of these observations is discussed. The results indicate that stimulated human chondrocytes are capable of producing active oxygen species which could play a major role in joint inflammation and cartilage damage. [less ▲]

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See detailHomologous recombinant growth hormone and calcium metabolism in the tilapia oreochromis mossambicus, adapted to freshwater
Flik, G.; Atsma, W.; Fenwick, J. C. et al

in Journal of Experimental Biology (1993)

Homologous recombinant tilapia growth hormoe was tested for its effect on calcium metabolism in freshwater tilapia Oreochromis mossambicus. A comparison of the role of GH and prolactin in calcium ... [more ▼]

Homologous recombinant tilapia growth hormoe was tested for its effect on calcium metabolism in freshwater tilapia Oreochromis mossambicus. A comparison of the role of GH and prolactin in calcium regulation of the tilapia led to the conclusion that GH has specific calciopropic effects on freshwater Tilapia that differ from those of prolactin. [less ▲]

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See detailVidéographie à caractère historique : essai d'inventaire et Audiovisuel, histoire et enseignement : repères bibliographiques
Jadoulle, Jean-Louis ULg

in Histoire et Enseignement : Bulletin (1993), 44e année(n°4),

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See detailThe use of the soil map of Belgium in the assessment of landslide risk
Closson, Damien; Engels, Patrick ULg; Demaret, Xavier

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(3), 165172

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See detailLe nom du bison en grec et en latin
Purnelle, Gérald ULg

in Latomus : Revue d'Etudes Latines (1993), 52(3), 567-580

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See detailReaction Dynamics of the Dichlorocarbene Anion Using tandem Mass Spectrometry.
Leyh, Bernard ULg; Mélon, Pierre

in Organic Mass Spectrometry (1993), 28

The dissociation dynamics of CCl2- anions were studied under various internal energy conditions. This study involved the measurement of daughter-ion spectra and branching ratios and of the amount of ... [more ▼]

The dissociation dynamics of CCl2- anions were studied under various internal energy conditions. This study involved the measurement of daughter-ion spectra and branching ratios and of the amount of kinetic energy released (KER) on the fragments. The competition between C and Cl loss for CCl2- ions created by many of the following sequence of processes: CCl4->CCl2+->(G=Xe, Kr C6H6)->CCl2--> fragments (1); CCl4->(CCl2--+[Cl,Cl]+;CCl2-; CCl2- (Ne)-> Fragments (2); CCl4->(CCl2)2+ (C6H6)->CCl2--> fragments (3) was particularly investigated. For process (1), the amount of fragmentating CCl2- ions is 8-10 times larger with Kr than with Xe or C6H6. The KER for Cl loss is smaller than 0.20 eV with the three target gases used. For the loss of C, the KER is much larger (0.7 eV). This KER is, however, equal to 0.21 eV for the collisionally activated C loss in process (2). For this latter process, the C loss vs Cl loss branching ratio decreases with increasing internal energy. The KERs measured for process (3) are vary similar to those obtained for process (1). It is suggested that repulsive or slightly bound excited states which correlate directly to the Cl2-(X2Sigma+)+C asymptote and which are vibronically coupled to the ground state play an important role in these dissociation processes. [less ▲]

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See detailLésion du nerf spinal accessoire
WANG, François-Charles ULg; Laurent, Luc; CRIELAARD, Jean-Michel ULg

in Acta physiotherapeutica (1993)

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See detailNitric oxide: a new messenger in the brain.
Bruhwyler, J.; Chleide, E.; Liégeois, Jean-François ULg et al

in Neuroscience & Biobehavioral Reviews (1993), 17(4), 373-84

The important role played by nitric oxide (NO) in the central nervous system has largely been emphasized in the recent literature. It can originate at least from four different sources: the endothelium of ... [more ▼]

The important role played by nitric oxide (NO) in the central nervous system has largely been emphasized in the recent literature. It can originate at least from four different sources: the endothelium of cerebral vessels, the immunostimulated microglia and astrocytes, the nonadrenergic noncholinergic nerve, and the glutamate neuron. NO has been implicated in a large number of pathologies (such as neurotoxicity in Alzheimer's disease and Huntington's disease, cerebral ischemia, stroke, and anxiety) and also in normal physiological functions (such as memory and learning, regulation of the cerebrovascular system, modulation of the wakefulness, mediation of nociception, olfaction, food intake and drinking, regulation of noradrenaline, and dopamine release). The aim of this paper is to review and to integrate the most recent advances in our understanding of the roles of NO in the brain. [less ▲]

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See detailNUCLEOPHILIC ENANTIOSELECTIVE SYNTHESIS OF 6-[F-18]FLUORO-L-DOPA VIA 2 CHIRAL AUXILIARIES
Lemaire, Christian ULg; Plenevaux, Alain ULg; Cantineau, Robert et al

in Applied Radiation & Isotopes (1993), 44(4), 737-744

Asymmetric nucleophilic synthesis of 6-[F-18]fluoro-L-dopa was investigated in order to reach an enantiomeric excess of close to 100% of the L form of this amino acid. The radiochemical synthesis required ... [more ▼]

Asymmetric nucleophilic synthesis of 6-[F-18]fluoro-L-dopa was investigated in order to reach an enantiomeric excess of close to 100% of the L form of this amino acid. The radiochemical synthesis required [F-18]fluoride as fluorinating agent and regioselective nucleophilic substitution of commercially available 6-nitroveratraldehyde. The [F-18]fluorobenzaldehyde thus obtained was easily converted to the corresponding 2-[F-18]fluoro-4,5-dimethoxybenzyl bromide. This alkylating agent was added to the lithium enolates of 1-(S)-(-)camphor imine of t-butyl glycinate (1) and (S)-(-)- 1 -Boc-2-t-butyl-3-methyl-4-imidazolidinone [(S)- Boc-BMI] (2) in order to compare the enantiomeric excess of the L form obtained in each case with these two chiral inductors. The L-isomer of fluorodopa was isolated after H1 hydrolysis and HPLC purification in 5-10% radiochemical yield (decay corrected). The overall synthesis time was of 110 min. Through this synthetic pathway, the L-isomer of fluorodopa was obtained in 83% e.e with 1 and 96% e.e with 2 respectively, as determined by chiral HPLC. A practical three step preparative scale synthesis of 6-[F-19]fluoro-D,L-dopa is also presented. [less ▲]

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See detailCompte-rendu de l'ouvrage : Avignon au Moyen Age. Textes et documents
Xhayet, Geneviève ULg

in Moyen Age (Le) (1993), 99

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See detailAdemhalingsfunktie als eventueel limiterende factor voor de gezondheid, de produktie en prestaties bij de dieren
Lekeux, Pierre ULg

in Vlaams Diergeneeskundig Tijdschrift (1993), 62

After a description of the varions links of the oxygen line and a definition of the notion «limiting factor», this paper describes why the pulmonary function can become a limiting factor in some domestic ... [more ▼]

After a description of the varions links of the oxygen line and a definition of the notion «limiting factor», this paper describes why the pulmonary function can become a limiting factor in some domestic animals which have been intensively selected for improved economic or athletic performances [less ▲]

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See detailLe traitement des adénomes à prolactine : utilisation d'un nouvel agoniste dopaminergique à longue durée d'action, la cabergoline, chez 56 patients
Beckers, Albert ULg; Abs, R.; Demez, Pierre ULg et al

in Médecine et Hygiène (1993), 51(1990), 1978-1980

Nous avons utilisé la cabergoline, un nouvel agoniste dopaminergique, dans le traitement des adénomes hypophysaires à prolactine. Cinquante-six patients atteints de micro-adénome dans 35 cas et de ... [more ▼]

Nous avons utilisé la cabergoline, un nouvel agoniste dopaminergique, dans le traitement des adénomes hypophysaires à prolactine. Cinquante-six patients atteints de micro-adénome dans 35 cas et de macroadénome dans 21 cas ont été traités durant des périodes variant entre 1 et 17 mois à des doses de cabergoline variant entre 0,25 mg et 2 mg par semaine, prises à raison de 1 à 4 fois par semaine. Cette nouvelle substance nous est apparue extrêmement efficace et très bien tolérée [less ▲]

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See detailNeutrophilic Eccrine Hidradenitis. A Case Report
Nikkels, Arjen ULg; Hansen, Isabelle ULg; Collignon, Jacques ULg et al

in Acta Clinica Belgica (1993), 48(6), 397-400

Neutrophilic eccrine hidradenitis (NEH) is a rare neutrophilic dermatosis occurring most frequently during induction chemotherapy for a variety of malignancies. We report a case of NEH in a 41-year-old ... [more ▼]

Neutrophilic eccrine hidradenitis (NEH) is a rare neutrophilic dermatosis occurring most frequently during induction chemotherapy for a variety of malignancies. We report a case of NEH in a 41-year-old woman with acute myeloblastic leukemia under daunorubicin, cytarabine and etoposide chemotherapy. She developed red, tender and painful nodules on a shoulder. The lesions resolved spontaneously. Histological examination is mandatory as the clinical presentation of this dermatosis is highly polymorphic. Leukemia cutis, sepsis, deep fungal infection and Sweet's syndrome must be excluded as these implicate different therapies. [less ▲]

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See detailCloning and characterization of G protein-coupled receptors
Parmentier, M.; Libert, F.; Perret, J. et al

in Advances in Second Messenger and Phosphoprotein Research (1993), 28

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See detailExteroceptive suppression of temporalis muscle activity: methodological and physiological aspects.
Schoenen, Jean ULg

in Cephalalgia : An International Journal of Headache (1993), 13(1), 3-10

In recent years studies of the suppression of EMG activity in temporalis muscle induced by stimulation in the trigeminal territory have opened new perspectives in headache research. The various methods ... [more ▼]

In recent years studies of the suppression of EMG activity in temporalis muscle induced by stimulation in the trigeminal territory have opened new perspectives in headache research. The various methods that have been used in different laboratories are reviewed and some of the physiological modulations of temporalis exteroceptive suppression are described. Among different methods of recording, averaging 10 full-wave rectified EMG responses produces results with acceptable variability and discomfort. In order to obtain maximal responses the intensity of the stimulation should reach at least 20 mA. To avoid habituation of the second temporalis exteroceptive suppression period (ES2), the stimulation frequency has to be at 0.1 Hz or below. The level of voluntary contraction is not a critical variable as long as it reaches 50% of maximum. Some physiological variations of temporalis suppression are well documented. In females, ES2 is shorter during menstruation than at mid-cycle and correlated with the estradiol/progesterone ratio in plasma. Conditioning temporalis ES2 by a preceding peripheral stimulus markedly reduces its duration, which is partly reversible by naloxone. Various pharmacological agents are able to modify temporalis ES2: its duration is increased by 5-HT1 antagonists, but decreased by 5-HT uptake blockers; contradictory results have been obtained with acetylsalicylic acid. These results suggest that inhibitory brain-stem interneurons mediating temporalis ES2 are inhibited by serotonergic afferents, probably from the raphe magnus nucleus, and that the latter receives an excitatory input from the periaqueductal gray matter and other limbic structures, in part via opioid receptors. [less ▲]

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See detailUn historique de la carte géologique de la Belgique
Boulvain, Frédéric ULg

in Professional paper Belgian geological survey (1993), 262

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See detailRestriction Maps of the DNA of Cervid Herpesvirus 1 and Cervid Herpesvirus 2, Two Viruses Related to Bovine Herpesvirus 1
Vanderplasschen, Alain ULg; Bublot, M.; Pastoret, Paul-Pierre ULg et al

in Archives of Virology (1993), 128(3-4), 379-88

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as ... [more ▼]

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1 DNA as probes. [less ▲]

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See detailApports récents de la génétique dans les maladies inflammatoires intestinales
Louis, Edouard ULg; Belaiche, Jacques ULg; Franchimont, D. et al

in Acta Clinica Belgica (1993), 48(6), 383-91

It is clear since many years that the liability to develop inflammatory bowel disease is influenced by genetic factors. Recent progresses in the field of genetic markers and genetic models bring the ... [more ▼]

It is clear since many years that the liability to develop inflammatory bowel disease is influenced by genetic factors. Recent progresses in the field of genetic markers and genetic models bring the concept of genetic heterogeneity. This new concept leads us to try to classify different forms of inflammatory bowel disease and could have in the future an impact on the prevention, the follow up and the treatment of these diseases. [less ▲]

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See detailAnalytical calculation of the electrostatic interaction energy within the CNDO framework
Dehareng, Dominique ULg; Dive, Georges ULg; Ghuysen, Jean-Marie ULg

in International Journal of Quantum Chemistry (1993), 46(6), 711-734

This work analyses the adequacy of an analytical electrostatic energy formulation within the CNDO framework to predict the stable conformations of large molecular complexes. Comparisons are made with ab ... [more ▼]

This work analyses the adequacy of an analytical electrostatic energy formulation within the CNDO framework to predict the stable conformations of large molecular complexes. Comparisons are made with ab initio results for small systems such as water-formamide, methanol-water-imidazole, or guanine-cytosine and with AM1 results for two large systems: a molecular tweezer + the 9-methyladenine complex and a model active site of the alpha-chymotrypsin and its ligand complex. This approach is efficient in providing reliable conformers for large molecular systems in a very fast way. [less ▲]

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See detailKonie pociagowe ardenskie
Burny, Philippe ULg; Lebailly, Philippe ULg; Ensch, D. et al

in Przeglad Hodowlany (1993), (3/1993), 21-22

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See detailPotentialités de modulation de la réaction inflammatoire
Delannoy, I.; Miossec, P.; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (1993), 137

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See detailImmunodetection of RNA on ultra-thin sections incubated with polyadenylate nucleotidyl transferase.
Thiry, Marc ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1993), 41(5), 657-65

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and ... [more ▼]

A new method is described for locating RNA on ultra-thin sections. Sections of aldehyde-fixed, plastic-embedded cells were incubated in a medium containing polyadenylate nucleotidyl transferase (PnT) and biotinylated ATP. The labeled nucleotides bound to RNA at the surface of the ultra-thin sections were than visualized by an indirect immunogold labeling technique. The resulting labeling pattern was dependent on the presence of divalent cations in the PnT medium. The method revealed with great precision the specific RNA-containing structures within Ehrlich tumor cells. The method is applicable to Epon sections. However, the labeling intensity varies according to the fixation used. Best results were obtained on acetylated cell sections. The method can be combined with EDTA regressive staining. The in situ PnT method provides a very useful tool for pinpointing the precise location of RNA within biological material at the ultrastructural level. [less ▲]

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See detailThe oncoprotein Bcl-3 directly transactivates through kappa B motifs via association with DNA-binding p50B homodimers.
Bours, Vincent ULg; Franzoso, G.; Azarenko, V. et al

in Cell (1993), 72(5), 729-39

Bcl-3 is an I kappa B-related protein with ankyrin repeat motifs. Its gene is located at a site of recurrent translocations in a subset of B cell chronic lymphocytic leukemias. Bcl-3 associates tightly ... [more ▼]

Bcl-3 is an I kappa B-related protein with ankyrin repeat motifs. Its gene is located at a site of recurrent translocations in a subset of B cell chronic lymphocytic leukemias. Bcl-3 associates tightly with p50B (NFKB2, p52) homodimers in cells, and together these proteins form a ternary complex with DNA at kappa B sites. Such an association functionally leads to a novel and potent form of transactivation through the kappa B motif: the tethering of Bcl-3 to DNA via the p50B homodimers allows Bcl-3 to transactivate directly, while p50B homodimers alone cannot. Transactivation mediated by Bcl-3 requires two cooperating domains located amino- and carboxy-terminal to the ankyrin domain. Bcl-3 is localized to the nucleus, and a Bcl-3-p50B complex is detected in certain lymphoid cells. Our data reveal a novel role for Bcl-3, distinct from that of the inhibitor I kappa B. The results have implications for tumorigenesis. [less ▲]

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See detailUltrastructure of CD57+ cells isolated from human tonsils or blood.
Bouzahzah, F.; Heinen, Ernst ULg; Antoine, Nadine ULg et al

in European Journal of Morphology (1993), 31(1-2), 82-6

The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human ... [more ▼]

The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human tonsils and blood by microdissection, rosetting with sheep red blood cells and magnetic cell sorting (MACS) and examined the ultrastructural morphology of these cells. Clear differences were found in cell aspect: blood NK contained large granules which were not found in the tonsillar CD57+ cells. These latter appeared medium-sized and not fully activated. After immunolabeling, the tonsillar CD57+ cells were mainly found in the light zone of the germinal centers. [less ▲]

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See detailEffects of Neurochemical Lesions of the Preoptic Area on Male Sexual Behavior in the Japanese Quail
Bailhache, T.; Surlemont, C.; Balthazart, Jacques ULg

in Brain Research Bulletin (1993), 32(3), 273-83

Two experiments were carried out during which the noradrenergic neurotoxin, 5-amino-2,4-dihydroxy-alpha-methylphenylethylamine (5-ADMP) was applied to the brain of quail in order to evaluate the role of ... [more ▼]

Two experiments were carried out during which the noradrenergic neurotoxin, 5-amino-2,4-dihydroxy-alpha-methylphenylethylamine (5-ADMP) was applied to the brain of quail in order to evaluate the role of the noradrenergic system in the control of male copulatory behavior. In the first experiment, the ICV injection of 5-ADMP slightly enhanced the sexual behavior observed in testosterone (T)-treated castrated male quail. This brings additional support to the notion that norepinephrine tonically inhibits male copulatory behavior in quail. In the second experiment, 5-ADMP implanted directly into the preoptic area disrupted the restoration by T of copulatory behavior in castrated quail and, at the same time, produced a brain lesion that partly destroyed the sexually dimorphic medial preoptic nucleus, a previously established site of T action on behavior. These lesions produced by a high (presumably too high) concentration of neurotoxin provided an independent confirmation of effects previously observed after electrolytic lesions. Correlation analyses also confirmed that the medial part of the POM just rostral to the anterior commissure is more closely associated with copulatory behavior and may, therefore, represent a key center for steroid action on this behavior. [less ▲]

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See detailDinant/Anseremme: Trou Magrite
Noiret, Pierre ULg

in Chronique de l'Archéologie wallonne (1993), 1

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See detailEnzymatic hydrolysis of reconstituted dimyristoylphosphatidylcholine-apo A-I complexes.
Lins, Laurence ULg; Piron, S.; Conrath, K. et al

in Biochimica et Biophysica Acta (1993), 1151(2), 137-42

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form ... [more ▼]

Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form discoidal particles of defined dimensions. While the knowledge of the apo A-I sequence and secondary structure has been used to make predictions about its mode of association with lipids, the available experimental data necessary to propose a precise model of these discoidal structures are still limited. An important step in our understanding of these structures would be to identify the apolipoprotein lipid-associated domains. Proteolysis of apo A-I-DMPC reconstituted HDL (rHDL) and free apo A-I is used here to identify lipid-protected domains of apo A-I. Free cleaved peptides were separated from rHDL associated peptides by density gradient centrifugation. The lipid-associated peptides were further analyzed by SDS-PAGE and transferred by Western blot to a ProBlott membrane for sequencing. Cleavage occurred at residue 43 with proteinase K, 46 with trypsin and residue 47 or 48 with pronase. A large domain from about residue 45 to the C-terminal remains highly protected against hydrolysis eventhough it contains several bonds susceptible to proteolytic cleavage. No protected fragments were detected by SDS-PAGE after enzymatic cleavage of free apo A-I in identical experimental conditions. [less ▲]

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See detailProductive Performance of the French Insurance Industry
Fecher-Bourgeois, Fabienne ULg; Kessler, D.; Perelman, Sergio ULg et al

in Journal of Productivity Analysis (1993), (4), 77-93

The purpose of this paper is to provide for both life and non-life insurance an assessment of the relative productive performance of French companies. We use parametric and nonparametric approaches to ... [more ▼]

The purpose of this paper is to provide for both life and non-life insurance an assessment of the relative productive performance of French companies. We use parametric and nonparametric approaches to construct a frontier to be used as a yardstick of productive efficiency. Our data basis covers 84 life and 243 non-life companies for the period 1984-1989. The main findings show a high correlation between parametric and nonparametric results and a wide dispersion in the rates of inefficiency across companies. This dispersion can be reduced when controlling for variations in scale, ownership, distribution, reinsurance, and claims ratios. [less ▲]

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See detailKaberdoes
Vromans, Joseph ULg

in Nederlands Van Nu (1993), 41(5),

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See detailModeling of the chemical processes in a longwall face underground gasifier at great depth
COEME, Antoinette; Pirard, Jean-Paul ULg; MOSTADE, Marc

in In Situ (1993), 17(1), 83-104

In order to simulate the cavity growth of an underground gasifier in thin seams, at great depth, Wilks1,2 has developed an original model based on the distribution of fluid flows around an injection well ... [more ▼]

In order to simulate the cavity growth of an underground gasifier in thin seams, at great depth, Wilks1,2 has developed an original model based on the distribution of fluid flows around an injection well. In this paper, we aim to introduce within this model a description of chemical processes on the wall and within the fluid phase, in order to determine the temperature and concentration profiles along the coal wall. These profiles are dependent only on two factors: the transfer coefficients and the energy balance of the gas generator. A study of the sensitivity of the operating variables has been carried out. [less ▲]

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See detailA new topical treatment for resistant herpes simplex infections.
Snoeck, R.; Andrei, G.; De Clercq, E. et al

in New England Journal of Medicine [=NEJM] (1993), 329(13), 968-9

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See detailCompte-rendu de J. Cerny : Papyrus hiératiques de Deir-el-Médineh
Winand, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1993), 78

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See detailMorphometric study of the stromal vascularization in peritoneal endometriosis.
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Anaf, Vincent et al

in Fertility and Sterility (1993), 59(3), 681-4

To evaluate the stromal vascularization of different appearances of peritoneal endometriosis, biopsies were taken from peritoneal areas with endometriosis in a series of 135 infertile women and classified ... [more ▼]

To evaluate the stromal vascularization of different appearances of peritoneal endometriosis, biopsies were taken from peritoneal areas with endometriosis in a series of 135 infertile women and classified as typical (black), red, or white lesions. The number of capillaries per mm2 of stroma, their mean surface area, and the ratio of capillaries/stroma surface area, and the mitotic activity were analyzed in typical, red, and white lesions. Significant differences were found between the different subgroups. The higher vascularization and mitotic activity observed in red lesions suggested the hypothesis that such lesions are very active and probably the first stage of early implantation of endometrial glands and stroma. The poor vascularization and the absence of mitosis observed in white lesions suggested that these lesions are much less active than red lesions and are a quiescent stage of the disease. Our study proves that the "activity" of peritoneal endometriosis is related to the vascularity. This concept must be considered in the further discussion of American Fertility Society Endometriosis Classification. Typical, red and white lesions are three different stages of the peritoneal disease and their relative relation to infertility is also probably different. [less ▲]

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See detailAssociation between the effacing (eae) gene and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle
Mainil, Jacques ULg; Jacquemin, E.; Kaeckenbeeck, A. et al

in American Journal of Veterinary Research (1993), 54

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See detailTransmission du virus BVD/MD et aspects épidémiologiques de l'infection
Boulanger, D.; Quatpers, D.; Mignon, Bernard ULg et al

in Bulletin des Groupements Techniques Vétérinaires (1993), 4

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See detailControl of the illegal administration of natural steroid hormones in urine and tissues of veal calves and in plasma of bulls
Scippo, Marie-Louise ULg; Gaspar, Pol; Degand, Guy ULg et al

in Analytica Chimica Acta (1993), 275(1-2), 57-74

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See detailHeightened selectivity in aromatic nitrations and chlorinations by the use of solid supports and catalysts
Delaude, Lionel ULg; Laszlo, Pierre ULg; Smith, Keith

in Accounts of Chemical Research (1993), 26(12), 607-613

The heightened selectivity in aromatic nitrations and chlorinations using solid supports and catalysts is reviewed with 67 references.

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See detailModifications of the extracellular matrix of aneurysmal abdominal aortas as a function of their size.
SAKALIHASAN, Natzi ULg; HEYERES, Antoine ULg; Richelle, Betty ULg et al

in European Journal of Vascular Surgery (1993), 7(6), 633-7

Collagen and elastin are the main extracellular matrix proteins providing the aortic wall with adequate mechanical properties and resistance for proper function. Our study aimed at investigating the ... [more ▼]

Collagen and elastin are the main extracellular matrix proteins providing the aortic wall with adequate mechanical properties and resistance for proper function. Our study aimed at investigating the relationship between the elastin concentration of the wall of normal and aneurysmal abdominal aortas (AAA), the collagen concentration, and its extractability, as a function of their size. Infrarenal aortas were collected from 30 patients undergoing operative repair of abdominal aortic aneurysm. Age-matched control samples were obtained from eight autopsies of individuals without vascular disease. Samples were divided into five groups according to the aortic diameter: control group (group N, n = 8); < 50 mm (group I, n = 6; between 50-75 mm (group II, n = 10); > 75 mm (group III, n = 7); and ruptured (group IV, n = 7). The collagen concentration in samples from group I was similar to the controls. An increased collagen concentration was observed in group II and remained at the same level in the largest and ruptured aneurysms. Extractability of collagen was found to be increased in group III and was even higher in group IV. A highly significant reduction in elastin concentration was observed in group I and there was progressive reduction with increasing diameter and rupture. A significant correlation could be established between aortic diameter, increased collagen extractability and decreased elastin content. [less ▲]

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See detailSolid-state nuclear magnetic resonance studies of the transformation of the zeolite Y catalyst in the course of hydrochlorination of 1-methylcyclohexene by thionyl chloride
Kolodziejski, Waclaw; Delaude, Lionel ULg; Laszlo, Pierre ULg et al

in Applied Catalysis A : General (1993), 98(1), 71-79

1-Methylcyclohexene was hydrochlorinated with SOCl2 to 1-chloro-1-methylcyclohexane at room temperature in CH2Cl2 as the solvent and in presence of the faujasitic zeolite ZF520 catalyst. 4-Coordinated ... [more ▼]

1-Methylcyclohexene was hydrochlorinated with SOCl2 to 1-chloro-1-methylcyclohexane at room temperature in CH2Cl2 as the solvent and in presence of the faujasitic zeolite ZF520 catalyst. 4-Coordinated framework aluminium in the parent sample coexisting with a small amount of immobile extra-framework aluminium at ca. -1 ppm is completely removed after just one reaction run and transformed into two distinct 6-coordinated species resonating at 0.2 and -3.1 ppm. The former line is assigned to Al(H2O)63+ cations, the latter is likely to come from a species with mixed solvation shells, probably Al(Cl-)(H2O)52+ and Al(OH-)(H2O)52+. Crystallinity of the catalyst is seriously affected by the reaction. The spectra confirm that before reaction the zeolite contains Si(3Si,1OH) and Si(2Si,2OH) groups. During the reaction most hydroxyl groups created by dealumination are removed, although some Si(3Si,1OH) remain. MAS NMR spectra are fully consistent with the proposed reaction mechanism. [less ▲]

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See detailOkadaic acid, a protein phosphatase inhibitor, enhances transcription of a receptor gene containing sequence A of the human prolactin promoter
Wera, S.; Belayew, A.; Martial, Joseph ULg

in Molecular Endocrinology (1993), 7(8), 965-71

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter ... [more ▼]

Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL promoter. We have investigated whether protein phosphatases could be involved in this regulation. GC-type rat pituitary tumor cells were transfected with sequence -138 to -35 of the hPRL gene promoter, upstream from a thymidine kinase promoter and a chloramphenicol acetyltransferase (CAT) reporter gene. Addition of okadaic acid (OA), a specific inhibitor of protein phosphatases 1 and 2A, stimulates transient expression of the CAT gene. The dose-response curve shows a maximal effect at 25 nM OA (2.2-fold stimulation above controls). The OA effect is also observed with a natural 4500-base pair hPRL promoter. A single copy of the hPRL promoter sequence -115 to -85 (sequence A) confers to a thymidine kinase-CAT construct an identical response to OA, whereas a single copy of the proximal Pit-1 binding site does not. Synergism is observed between cAMP and OA in activating PRL gene transcription. This synergism is also observed with a single copy of sequence A. The effect of cAMP is not mediated by an L-type Ca2+ channel, since addition of the Ca2+ channel antagonist verapamil does not decrease it, nor does complexing extracellular Ca2+ significantly reduce it. Furthermore, OA and the Ca2+ channel opener BAY K8644 exert additive effects. [less ▲]

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See detailEmperipolesis of lymphoid cells by human follicular dendritic cells in vitro.
Tsunoda, R.; Nakayama, M.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailRetinoic acid induction of major histocompatibility complex class I genes in NTera-2 embryonal carcinoma cells involves induction of NF-kappa B (p50-p65) and retinoic acid receptor beta-retinoid X receptor beta heterodimers.
Segars, J. H.; Nagata, T.; Bours, Vincent ULg et al

in Molecular & Cellular Biology (1993), 13(10), 6157-69

Retinoic acid (RA) treatment of human embryonal carcinoma (EC) NTera-2 (NT2) cells induces expression of major histocompatibility complex (MHC) class I and beta-2 microglobulin surface molecules. We found ... [more ▼]

Retinoic acid (RA) treatment of human embryonal carcinoma (EC) NTera-2 (NT2) cells induces expression of major histocompatibility complex (MHC) class I and beta-2 microglobulin surface molecules. We found that this induction was accompanied by increased levels of MHC class I mRNA, which was attributable to the activation of the two conserved upstream enhancers, region I (NF-kappa B like) and region II. This activation coincided with the induction of nuclear factor binding activities specific for the two enhancers. Region I binding activity was not present in undifferentiated NT2 cells, but binding of an NF-kappa B heterodimer, p50-p65, was induced following RA treatment. The p50-p65 heterodimer was produced as a result of de novo induction of p50 and p65 mRNAs. Region II binding activity was present in undifferentiated cells at low levels but was greatly augmented by RA treatment because of activation of a nuclear hormone receptor heterodimer composed of the retinoid X receptor (RXR beta) and the RA receptor (RAR beta). The RXR beta-RAR beta heterodimer also bound RA responsive elements present in other genes which are likely to be involved in RA triggering of EC cell differentiation. Furthermore, transfection of p50 and p65 into undifferentiated NT2 cells synergistically activated region I-dependent MHC class I reporter activity. A similar increase in MHC class I reporter activity was demonstrated by cotransfection of RXR beta and RAR beta. These data show that following RA treatment, heterodimers of two transcription factor families are induced to bind to the MHC enhancers, which at least partly accounts for RA induction of MHC class I expression in NT2 EC cells. [less ▲]

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See detailConnection and frame design for economy
Anderson, D.; Colson, A.; Jaspart, Jean-Pierre ULg

in Stavební Obzor (1993), 10

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See detailAnalyse des comportements d'entraîneurs de volley-ball pendant des rencontres officielles
Cloes, Marc ULg; Delhaes, Jean-Pierre; Piéron, Maurice

in Sport (1993), 141(1), 16-25

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se ... [more ▼]

L'observation systématique des comportements des entraîneurs sportifs a connu un réel développement au cours de la dernière décennie. Les études relevées dans la littérature spécialisée peuvent se regrouper selon leurs objectifs: (1) l'analyse descriptive des comportements d'entraîneurs et de sportifs, parfois de très haut niveau; (2) la comparaison de situations pédagogiques, notamment en observant une même personne exerçant les fonctions d'entraîner et d'enseignant; (3) la comparaison des interventions des entraîneurs en fonction du niveau d'habileté des sportifs qu'ils dirigent; (4) la comparaison du comportement des entraîneurs en fonction des succès ou des échecs de leur équipe. ... [less ▲]

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See detailExemples d'utilisation de la Carte des Sols de Belgique comme base d'un systeme d'information geographique pour la gestion des terroirs.
Engels, Patrick ULg; Bock, Laurent ULg; Neven, Cécile et al

in Bulletin des Recherches Agronomiques de Gembloux (1993), 28(2-3),

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See detailStructure of the apolipoprotein A-IV/lipid discoidal complexes: an attenuated total reflection polarized Fourier transform infrared spectroscopy study.
Lins, Laurence ULg; Brasseur, Robert ULg; Rosseneu, M. et al

in Biochimica et Biophysica Acta (1993), 1149(2), 267-77

Discoidal lipid particles were prepared from a reaction mixture containing apo A-IV and dimyristoylphosphatidylcholine (DMPC) or dipalmitoylphosphatidylcholine (DPPC) in the molar ratio of 185:1 (lipid ... [more ▼]

Discoidal lipid particles were prepared from a reaction mixture containing apo A-IV and dimyristoylphosphatidylcholine (DMPC) or dipalmitoylphosphatidylcholine (DPPC) in the molar ratio of 185:1 (lipid/protein). The complexes were isolated by gel filtration and characterized in terms of composition and size. Infrared attenuated total reflection spectroscopy was used to estimate the secondary structure of apolipoprotein A-IV and the orientation of its amphipathic alpha-helices with respect to the lipid hydrocarbon chains. In addition, infrared spectra were analyzed in terms of the conformation and organization of different regions of the lipid molecules in the particles. This approach has been applied successfully to reconstituted HDL particles prepared from a reaction mixture containing DPPC and apo A-I in the molar ratio of 150:1 (Wald, J.H., Goormaghtigh, E., De Meutter, J., Ruysschaert, J.M. and Jonas, A. (1990) J. Biol. Chem. 265, 20044-20050). Apo A-IV helicity increased for the protein bound to DMPC or DPPC but the increase was more pronounced for the apo A-IV/DMPC particles. In both complexes, the alpha helical amphipathic segments of the protein were parallel to the lipid acyl chains and no significant modification of the overall organization of the lipid molecules in the lipid bilayer was observed. The presence of apo A-IV seems only to affect the conformation of the lipid hydrocarbon chains in close contact with the protein in the discoidal particles. [less ▲]

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See detailComparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo
Ectors, Fabien ULg; Thonon, Fabienne; Delval, Alain et al

in Livestock Production Science (1993), 36

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC ... [more ▼]

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC conditioned medium (CM) or in rabbit oviducts, versus in vivo produced embryos. There was no significant difference in terms of percentages of cleaved and 8-cell stages obtained between CM and BOEC. In CM, 24.8% of the cleaved embryos became blastocysts. In BOEC, 14.5% of the cleaved embryos became blastocysts. Among the 190 zygotes transferred in the rabbit oviducts, 127 have been recovered 5 days later, and 17.4% became blastocysts. There was no significant difference in term of blastocyst formation between the development in rabbit and in BOEC. However, there was a significant difference between the CM group and the two other groups. The numbers of cells in blastocysts from different sources were investigated: in vivo blastocysts contained 107 cells assumed to be 100%, in vitro blastocysts developed in rabbit oviduct 100.1 cells (93.3%), BOEC blastocysts 90.8 cells (84.6%) and CM blastocysts 72.3 cells (67.3%). This study confirmed earlier works on the oviduct effect on blastocyst quality in terms of development rate and cell number. [less ▲]

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See detailThe oncoprotein Bcl-3 can facilitate NF-kappa B-mediated transactivation by removing inhibiting p50 homodimers from select kappa B sites.
Franzoso, G.; Bours, Vincent ULg; Azarenko, V. et al

in EMBO Journal (1993), 12(10), 3893-901

Previously we have proposed a role for Bcl-3 in facilitating transactivation through kappa B sites by counteracting the inhibitory effects of bound, non-transactivating homodimers of the p50 subunit of NF ... [more ▼]

Previously we have proposed a role for Bcl-3 in facilitating transactivation through kappa B sites by counteracting the inhibitory effects of bound, non-transactivating homodimers of the p50 subunit of NF-kappa B. Such homodimers are abundant for example in nuclei of unstimulated primary T cells. Here we extend the model and provide new evidence which fulfills a number of predictions. (i) Bcl-3 preferentially targets p50 homodimers over NF-kappa B heterodimers since the homodimers are completely dissociated from kappa B sites at concentrations of Bcl-3 which do not affect NF-kappa B. (ii) Select kappa B sites associate very strongly and stably with p50 homodimers, completely preventing binding by NF-kappa B. Such kappa B sites are likely candidates for regulation by p50 homodimers and Bcl-3. (iii) Bcl-3 and p50 can be co-localized in the nucleus, a requirement for active removal of homodimers from their binding sites in vivo. (iv) The ankyrin repeat domain of Bcl-3 is sufficient for the reversal of p50 homodimer-mediated inhibition, correlating with the ability of this domain alone to inhibit p50 binding to kappa B sites in vitro. Our data support the model that induction of nuclear Bcl-3 may be required during cellular stimulation to actively remove stably bound p50 homodimers from certain kappa B sites in order to allow transactivating NF-kappa B complexes to engage. This exact mechanism is demonstrated with in vitro experiments. [less ▲]

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See detailCompte rendu de Aux sources du monachisme colombanien
George, Philippe ULg

in Moyen Age (Le) (1993)

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