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See detailLa protéine P53, protectrice de l'intégrité du génome: rôle dans la genèse des cancers
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Gielen Jacques et al

in Revue Médicale de Liège (1994), 49(5), 274-84

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et ... [more ▼]

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et déterminent leur appartenance tissulaire. Des données récentes indiquent que l'apparition des cancers résulte du déséquilibre de cet état dynamique soit part l'activation de facteurs positifs désignés sous le terme d'oncogènes, soit par l'inactivation de facteurs négatifs qui sont regroupés dans la famille des gènes suppresseurs de tumeurs. Le gène codant pour la protéine p53 est un membre particulièrement important de cette dernière famille. En effet, son inactivation, par mutation et/ou délétion, est l'une des altérations génétiques la plus fréquemment détectée dans les cancers. La fonction principale attribuée à la protéine p53 consiste à préserver le génome des altérations susceptibles d'entraîner, entre autres, la cellule dans un processus de transformation maligne. C'est en permettant la réparation des altérations du DNA survenues lors de sa réplication avant la mitose ou provoquées par des agents extérieurs, que la protéine p53 semble exercer ses fonctions. Des altérations du gène p53 entraînant des perturbations de la fonction de la protéine p53 ont été identifiées au niveau de la plupart des lésions tumorales malignes. A ce titre, cette protéine semble jouer un rôle déterminant au niveau de la genèse des cancers. Aussi le gène p53 fait-il l'objet de recherches intensives qui devraient déboucher sur la mise au point de nouveaux moyens de détection et d'évaluation pronostique des cancers. D'autre part, des expériences visant à restaurer la fonction de la protéine p53 au niveau des cellules cancéreuses ouvrent de nouvelles et séduisantes perspectives thérapeutiques. [less ▲]

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See detailEfficacy and Tolerability of a New Formulation of Oral Tiludronate (Tablet) in the Treatment of Paget's Disease of Bone
Reginster, Jean-Yves ULg; Treves, R.; Renier, J. C. et al

in Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research (1994), 9(5), 615-9

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial ... [more ▼]

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial. Patients received a daily dose of 400 mg oral tiludronate (two tablets). Treatment was for 6 months. Serum alkaline phosphatase activity (SAP) and fasting urinary excretion of hydroxyproline/creatine (OH/Cr) were measured every 3 months, as were biochemical parameters reflecting renal, hepatic, and hematologic functions. Analgesic efficacy was self-evaluated from a visual analog scale (VAS). Statistical analysis revealed a significant reduction from baseline in SAP and OH/Cr levels, as well as VAS scores. In the whole population with evaluation under treatment, there was a reduction in initial SAP activity after 3 months (47.2 +/- 2.2%, mean +/- SEM) and 6 months (58.3 +/- 2.3%). In the population with SAP levels above twice the upper limit at inclusion and with evaluation at month 3 and month 6 (n = 96), the reduction in SAP levels was 49.3 +/- 2.4% after 3 months and of 59.5 +/- 2.6% after 6 months (ANOVA time effect, p = 0.0001). Aside from mild gastrointestinal disturbances, as experienced with other oral bisphosphonates, clinical tolerance was good. Exhaustive biochemical investigation failed to reveal significant toxicity of tiludronate tablets at the dose of 400 mg/day. The dose of 400 mg daily of this new formulation appears to be a satisfactory tiludronate regimen for the treatment of Paget's disease of bone. [less ▲]

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See detailMacrophages and Tumor Necrosis Factor Alpha in Toxic Epidermal Necrolysis
Paquet, Philippe ULg; Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg et al

in Archives of Dermatology (1994), 130(5), 605-608

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell ... [more ▼]

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell infiltrate varied according to the stage of the disease. The main constant feature was the prominent involvement of the monocyte-macrophage lineage, including factor XIIIa+HLA-DR+ dendrocytes and CD68+ Mac 387+ macrophages, before and during the epidermal necrosis. The number of CD4+ and CD8+ lymphocytes was comparatively small. This was associated with a dense labeling of the epidermis for tumor necrosis factor alpha. CONCLUSIONS: Cells of the monocyte-macrophage lineage largely outnumber lymphocytes in the lesions of toxic epidermal necrolysis. Tumor necrosis factor alpha is likely a major cytokine that is responsible for necrosis. [less ▲]

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See detailDirect NMR evidence for a local resriction in the segmental chain mobility of a model ionomer
Vanhoorne, Pierre; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(9), 2548-2552

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which ... [more ▼]

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which the chain segments bearing the sodium carboxylate end groups have been selectively protonated; the second one has a protonated polystyrene backbone terminated at both ends with a deuterated polystyrene segment bearing the sodium carboxylate groups. Both polymers have been studied by high-resolution solid-state C-13 NMR. Line-width measurements of the protonated units have clearly shown that the dipolar interactions of the sodium carboxylate end groups are responsible for a mobility restriction of the chain end segments. This experiment is thought to be the first direct evidence for the model recently proposed by Eisenberg et al. for ionomer morphology according to which the ionic multiplets are surrounded with a shell of constrained polymer segments. [less ▲]

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See detailAgm-1470, a Potent Angiogenesis Inhibitor, Prevents the Entry of Normal but Not Transformed Endothelial Cells into the G1 Phase of the Cell Cycle
Antoine, Nadine ULg; Greimers, Roland ULg; De Roanne, C. et al

in Cancer Research (1994), 54(8), 2073-6

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this ... [more ▼]

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this molecule appears to be a most promising anticancer drug, its mechanism of action has not yet been elucidated. In this study, we examined the effects of AGM-1470 on the cell cycle of normal and transformed endothelial cells. We showed that AGM-1470, at picomolar concentrations, specifically inhibits the proliferation of both bovine aortic endothelial cells and human umbilical vein endothelial cells. AGM-1470 was ineffective in significantly inhibiting the proliferation of Ea.hy926 cells, a hybrid cell line obtained by the fusion of human umbilical vein endothelial cells with a human carcinoma cell line, or cEnd.1 cells, a polyoma middle T oncogene-transformed endothelioma cell line derived from mouse embryo. Using a double labeling technique with anti-Ki67 antibodies and propidium iodide, we demonstrated, with flow cytometry analysis, that AGM-1470 specifically prevents the entry of endothelial cells into the G1 phase of the cell cycle. We also showed that AGM-1470 was ineffective in inhibiting endothelial cell migration toward laminin or capillary-like tube formation inside a type I collagen matrix induced by phorbol esters. Our data strongly suggest that AGM-1470 is a molecule that specifically inhibits a cell cycle control pathway active in normal cells but which could be bypassed or altered in transformed cells. [less ▲]

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See detail4 YEARS RESEARCH ON THE REPRODUCTIVE-BEHAVIOR AND HYBRIDIZATION OF BARBUS-BARBUS AND BARBUS-MERIDIONALIS
Poncin, Pascal ULg

in Bulletin Français de la Pêche et de la Pisciculture (1994), 334

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The ... [more ▼]

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The reproductive behaviour is described as well as the behavioural mechanisms of hybridization. During the spawning act - sometimes preceded by <<forehead swim>> in which two fish swim together, at high speed, head against head the female and the few accompanying males rise from the gravel and move their genital papilla in the gravel while releasing ova or sperm (fig. 1 and 2). The reproductive behaviours of Barbus barbus and Barbus meridionalis appear to be similar and hybridizations were observed, showing that no complete behavioural barrier blocks the mating of the two species (fig. 2). The daily rhythms of activity are compared within the two species. The influence of temperature and daylength on the daily rhythms of activity is discussed. in winter conditions, barbels exhibit a night activity rhythm. Spawning occurs during daytime or during the night (fig. 3). The influence of spawning stimuli is analysed using dummies of different sizes and colors (photo I). Barbels seem to prefer white, grey, yellow and brown colors to red or black colors. [less ▲]

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See detailKnowledge-Based System for the Automated Solid-Phase Extraction of Basic Drugs from Plasma Coupled with Their Liquid Chromatographic Determination. Application to the Biodetermination of Beta-Receptor Blocking Agents
Hubert, Philippe ULg; Chiap, Patrice ULg; Moors, M. et al

in Journal of Chromatography. A (1994), 665(1), 87-99

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges ... [more ▼]

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges (DECs) by means of a sample processor. A knowledge-based system was developed to facilitate the development of fully automated methods for the solid-phase extraction of relatively hydrophobic basic drugs from plasma, coupled with their determination by high-performance liquid chromatography (HPLC). The DEC filled with 50 mg of cyanopropyl-bonded silica phase is first conditioned with methanol and buffer solution (pH 7.4). After sample application, the DEC sorbent is washed with the same buffer. The analytes are then desorbed with an appropriate eluent and the eluate is finally diluted with the same buffer as used in the HPLC mobile phase before injection. Under these conditions, only three variables are still to be optimized: the composition and volume of the elution solvent and the volume of buffer to be added to the eluate. On the basis of this general strategy, a decision tree providing information about suggested starting conditions and guidelines for the optimization of the three variables was developed and implemented by use of a hypermedia software. This didactic expert system was evaluated using several beta-receptor blocking agents as model compounds and the operating conditions obtained for the automated SPE of these compounds are presented. A method for the determination of propranolol in plasma using the SPE conditions deduced from the knowledge-based system was validated. The absolute recovery of propranolol is ca. 93% and the limit of detection is 1.3 ng ml-1. The mean within-day and between-day reproducibilities are 2.3 and 3.6%, respectively. [less ▲]

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See detailIncreased Parasellar Activity on Gallium Spect Is Not Specific for Active Cluster Headache
Sianard-Gainko, J.; Milet, J.; Ghuysen, V. et al

in Cephalalgia : An International Journal of Headache (1994), 14(2), 132-3

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients ... [more ▼]

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients, 54% of episodic CH patients in an active period, 56% of episodic CH patients in remission and 71% of migraineurs. No significant correlations were found between the SPECT images and the duration of the disease, of cluster periods or of remissions. Increased parasellar activity on Gallium SPECT is thus not specific for CH, nor for the active period of episodic CH. The method lacks reliability for investigation of putative cavernous sinus inflammation. [less ▲]

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See detailComparison of Cardiac Function in Double-Muscled Calves and in Calves with Conventional Muscular Conformation
Amory, Hélène ULg; Desmecht, Daniel ULg; Linden, Annick ULg et al

in American Journal of Veterinary Research (1994), 55(4), 561-6

During growth, central venous, right ventricular, pulmonary arterial, pulmonary capillary wedge, and systemic arterial pressures, heart rate, and cardiac output were repeatedly measured in 41 Friesian ... [more ▼]

During growth, central venous, right ventricular, pulmonary arterial, pulmonary capillary wedge, and systemic arterial pressures, heart rate, and cardiac output were repeatedly measured in 41 Friesian calves, considered as having conventional muscular conformation, and in 19 Belgian White and Blue double-muscled calves. A total of 123 and 70 recordings were collected in conventional and double-muscled calves, respectively. These circulatory indices were calculated: stroke volume, cardiac and stroke indices, pulmonary and systemic pulse pressures, pulmonary and systemic vascular resistance indices, and right and left ventricular work indices. Results indicated that systemic arterial and pulse pressures, as well as cardiac output, stroke volume, cardiac and stroke indices, and right and left ventricular work indices were significantly (P < or = 0.05 to 0.001) lower but, in contrast, pulmonary and systemic vascular resistance indices were significantly (P < or = 0.001) higher in double-muscled than in conventional calves. Right-sided vascular pressures and heart rate were similar in the 2 groups. These results indicated that global cardiac performance may be considerably poorer in double-muscled calves. Diminished cardiac performance of double-muscled calves appears to be related neither to relative bradycardia nor to reduced ventricular preload. The potential role of increased ventricular afterload or of reduced myocardial contractility in double-muscled cattle should be determined by direct measurements. [less ▲]

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See detailLa prévention de l'ostéoporose postménopausique en cas de contre-indications des œstrogènes
Reginster, Jean-Yves ULg

in Revue Médicale de Liège (1994), 49(4), 194-8

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See detailA new family of "ligated" anionic initiators for the "living" polymerization of (meth)acrylic esters
Bayard, Philippe; Jérôme, Robert ULg; Teyssié, Philippe et al

in Polymer Bulletin (1994), 32(4), 381-385

A new family of ligands (dual sigma - pi ligand), i.e. chelating lithium alkoxides, Li-O-(CH2-CH2O)nCH3, is shown to be very effective in promoting the living anionic Polymerization of methacrylic and ... [more ▼]

A new family of ligands (dual sigma - pi ligand), i.e. chelating lithium alkoxides, Li-O-(CH2-CH2O)nCH3, is shown to be very effective in promoting the living anionic Polymerization of methacrylic and acrylic esters, including a number of primary alkyl acrylates such as n-butyl acrylate and 2-ethylhexylacrylate. [less ▲]

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See detailFlow cytometry in prostate cancer: Histological and clinical correlation
de Leval, Laurence ULg; Waltregny, David ULg; Greimers, Roland ULg et al

in Acta Urologica Belgica (1994), 62(1), 37-43

DNA histograms were obtained by flow cytometry for 39 human prostate carcinomas (27 total prostatectomy specimens, 5 biopsies and 7 transuretral resections). The study was performed on formalin-fixed and ... [more ▼]

DNA histograms were obtained by flow cytometry for 39 human prostate carcinomas (27 total prostatectomy specimens, 5 biopsies and 7 transuretral resections). The study was performed on formalin-fixed and paraffin-embedded material. In this report, ploidy index did not seem to be a good marker of prognosis as no significant variation in ploidy was found neither among the different stages nor among the different Gleason categories. Proliferative index of the tumors seemed to be a more sensitive parameter; a significant relation was observed between proliferative index and stage of the tumor. The authors discuss these results under the light of previously reported observations. [less ▲]

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See detailExpression and Induction of Drug-Metabolizing Enzymes in Cultured Fetal Rat Hepatocytes
Kremers, Pierre ULg; Roelandt, L.; Stouvenakers, Nadine ULg et al

in Cell Biology and Toxicology (1994), 10(2), 117-25

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations ... [more ▼]

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations. Hepatocytes cultured up to 3 weeks in the presence of dexamethasone and phenobarbital still expressed some drug-metabolizing enzyme activities (e.g., ECOD). The enzymatic activities were measured both directly on monolayers during culture and on the corresponding harvested and homogenized cells. The results correlate perfectly with each other. The 'on cell' procedure allows us to repeat the assay or to measure several activities on the same cells at different time intervals. The presence of dexamethasone in the culture medium allows the expression and the induction of several cytochrome P450 isoenzymes, namely, those hydroxylating testosterone. This makes the model particularly attractive for induction experiments as well as for metabolic or toxicological studies needing longer treatments. [less ▲]

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See detailSex Difference in the Neurotensin-Immunoreactive Cell Populations of the Preoptic Area in Quail (Coturnix Japonica)
Absil, Philippe ULg; Balthazart, Jacques ULg

in Cell & Tissue Research (1994), 276(1), 99-116

The distribution of neurotensin-immunoreactive cells and fibers was analyzed by immunocytochemistry in the forebrain of male and female Japanese quail (Coturnix japonica) by using an antibody directed ... [more ▼]

The distribution of neurotensin-immunoreactive cells and fibers was analyzed by immunocytochemistry in the forebrain of male and female Japanese quail (Coturnix japonica) by using an antibody directed against the C-terminal part of the molecule. [less ▲]

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See detailPolitique et pouvoir
Verjans, Pierre ULg

in Revue Nouvelle (1994)

Las travaux publiés à Liège illustrent une nouvelle tendance à l'intérêt pour les études sur les migrations.

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See detailDiagnostic: L'apport du laboratoire
Nikkels, Arjen ULg; Rentier, Bernard ULg

in Virologics (1994), 1

Chez le sujet immunodéprimé (SIDA, greffe, cancer, médicaments), la femme enceinte et le nouveau-né, la gravité potentielle des infections herpétiques exige l'instauration rapide d'un traitement efficace ... [more ▼]

Chez le sujet immunodéprimé (SIDA, greffe, cancer, médicaments), la femme enceinte et le nouveau-né, la gravité potentielle des infections herpétiques exige l'instauration rapide d'un traitement efficace. Etant donné que, chez ces sujets, des formes atypiques rendent le tableau clinique souvent déroutant, il est indispensable de pouvoir disposer de techniques diagnostiques rapides et précises. [less ▲]

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See detailVers une Politique Européenne de l'Immigration, Research Paper n° 3
Martiniello, Marco ULg

in The Philip Morris Institute for Public Policy (1994)

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See detailCrime Prevention in Belgium: Towards "integrated prevention"
Kellens, Georges ULg; Comeron, Manuel ULg

in International Criminal Police Review (1994), (447), 7-15

Tragic events can provide the opportunity for an overall rethink of crime prevention. Recent history in belgium has led to crime prevention being recentred at local level for that is where it can be ... [more ▼]

Tragic events can provide the opportunity for an overall rethink of crime prevention. Recent history in belgium has led to crime prevention being recentred at local level for that is where it can be deployed most effectively. [less ▲]

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See detailPossible pathogenic role of IgE in Henoch-Schonlein purpura
Davin, J. C.; Pierard, Gérald ULg; Dechenne, Charles ULg et al

in Pediatric Nephrology : Journal of the International Pediatric Nephrology Association (1994), 8(2), 169-171

The incidences of clinical and biological markers of atopy were investigated in 16 children with IgA nephropathy (IgAN) (group A) and in 22 with Henoch-Schonlein purpura nephritis (HSPN) (group B). The ... [more ▼]

The incidences of clinical and biological markers of atopy were investigated in 16 children with IgA nephropathy (IgAN) (group A) and in 22 with Henoch-Schonlein purpura nephritis (HSPN) (group B). The incidence of increased plasma IgE levels according to age-matched normal values was significantly higher in group B (17/22, 77%) than in group A (7/16, 44%) (P < 0.05). Although not significant, the incidences of positive RAST tests and of a history of typical atopic symptoms were also higher in group B [10/22 (45%) and 11/22 (50%), respectively] than in group A [4/16 (25%) and 5/16 (31%), respectively]. Moreover, IgE deposits were demonstrated by a peroxidase/anti-peroxidase method on cutaneous Langerhans and mast cells in 4 of 6 patients with HSPN. Thus immunoallergy might account, in some cases, for the cutaneous, intestinal and pulmonary signs observed in HSPN, but not in IgAN. We postulate stimulation of IgE-sensitized mast cells by specific antigens in the presence of IgA circulating immune complexes (CIC), release of vasoactive substances, increased capillary permeability and perivascular deposition of IgA CIC. [less ▲]

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See detailSelective localization of carbon black in immiscible polymer blends : a useful tool to design electrical conductive composites
Gubbels, Frédéric; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(7), 1972-1974

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See detailBinding Site-Shaped Repeated Sequences of Bacterial Wall Peptidoglycan Hydrolases
Ghuysen, Jean-Marie ULg; Lamotte-Brasseur, Josette; Joris, Bernard ULg et al

in FEBS Letters (1994), 342(1), 23-28

The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of ... [more ▼]

The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of Bacillus licheniformis were previously reported to have similarities with the amino acid sequence of the non-catalytic N-terminal module of the Streptomyces albus G Zn DD-peptidase. This peptidase is a bipartite protein of known three-dimensional structure. Its non-catalytic N-terminal module possesses, exposed at the surface, an elongated crevice which is defined by a loop-helix-loop-helix motif that consists of two repeats, each 16 amino acid residues long, connected by a heptapeptide and whose design is compatible with its possible functioning as a substrate recognition and binding site. Amino acid alignments suggest that cavities nearly identical in shape to that present in the non-catalytic module of the S. albus peptidase, are borne by the C-terminal regions of the CwlA amidase (in one copy), the lysozyme and the ORFL3 and CwlL amidases (in two copies). Since a common feature of the five enzymes is their substrate, the bacterial cell wall peptidoglycan, we interpret the striking similarity of their non-catalytic N- or C-terminal modules to suggest that these modules are involved in the binding of these exocellular enzymes to their insoluble wall substrate. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 13. Carbon-13 NMR characterization of the mixed complexation of methyl α-lithioisobutyrate by lithium tert-butyoxide in tetrahydrofuran
Wang, Jin-Shan; Jérôme, Robert ULg; Warin, Roger et al

in Macromolecules (1994), 27(24), 1691-1696

The mixed complexation of methyl alpha-lithioisobutyrate(MIBLi)(a monomeric model structurally corresponding to the living end of PMMA-Li+) with lithium tert-butoxide (LiOtBu) in THF has been studied for ... [more ▼]

The mixed complexation of methyl alpha-lithioisobutyrate(MIBLi)(a monomeric model structurally corresponding to the living end of PMMA-Li+) with lithium tert-butoxide (LiOtBu) in THF has been studied for the first time by using C-13 NMR spectroscopy. It is shown that LiOtBu, like LiCl, is very effective in coordinating with MIBLi tetramer in THF, incrementally replacing MIBLi with the formation of other tetrameric complexes, MIBxLi4(OtBu)4-x (x = 1-3). Complextion of MIBLi by LiOtBu induces a shielding C-13 shift of the MIBLi C(alpha) resonance, which might suggest that lithium cations associated with the electron-rich ligands, i.e., LiOtBu, can also form weaker bonds with the carbonyl oxygen of MIBLi (i.e., mu-type complexes), thereby decreasing the localization of the negative charge on that latter atom. In comparison with LiCl, one of the most significant features is that only one single type of species results from mixed complexation of MIBLi and LiCl at LiCl/MIBLi = 1, 2, and greater-than-or-equal-to 3, respectively, at low temperature, whereas several LiOtBu-complexed species may coexist, whatever the LiOtBu/MIBLi ratio; furthermore, their exchange is found to be very slow at a temperature as high as 0-degrees-C. Tentatively, this striking feature may explain nicely the large difference in molecular weight distribution of poly(meth)acrylates anionically prepared in the presence of LiOtBu and LiCl, respectively. [less ▲]

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See detailLe médecin de famille et l'hôpital de jour: complémentarité de soins
Triffaux, Jean-Marc ULg; Halkin, Stéphane ULg; Bertrand, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(3), 133-40

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See detailManifestations cutanées des maladies pulmonaires
Corhay, Jean-Louis ULg; Bury, Thierry ULg; Louis, Renaud ULg et al

in Revue Médicale de Liège (1994), 49(3), 141-52

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See detailPotentiation of Histamine Release from Human Leucocytes by Paf
Louis, Renaud ULg; Bury, Thierry ULg; Corhay, Jean-Louis ULg et al

in Agents and Actions (1994), 41(1-2), 5-10

Studies on the effects of PAF on histamine release from human leucocytes have yielded conflicting results. We therefore investigated the effects of PAF on leucocytic histamine release (HR) focusing on ... [more ▼]

Studies on the effects of PAF on histamine release from human leucocytes have yielded conflicting results. We therefore investigated the effects of PAF on leucocytic histamine release (HR) focusing on direct as well as on modulating effects. Peripheral blood leucocytes of normal and atopic subjects were incubated with PAF, anti-IgE and FMP for 30 min at 37 degrees C, and histamine was measured fluorometrically. Unlike anti-IgE (1/2000) and FMP (10(-5) M) which caused histamine release (HR) of 34 +/- 7% and 31 +/- 8%, respectively, PAF by itself (10(-11)-10(-5) M) failed to induce any significant HR from human leucocytes (< 3%) in normal (n = 14) and atopic subjects (n = 6). Nevertheless, in normals as well as atopics, PAF, but not lyso-PAF, enhanced anti-IgE (1/2000) and FMP (10(-5) M)-induced HR in a concentration-related manner. Maximal potentiation of histamine release caused by FMP and anti-IgE was achieved with PAF (10(-7)) (mean +/- SEM: 26 +/- 5%, n = 5, p < 0.01) and PAF (10(-5)) (mean +/- SEM: 20 +/- 7%, n = 7, p < 0.05), respectively. This potentiation was suppressed by WEB2086 (10(-5) M), a specific PAF antagonist. The time course of the enhancing effect produced by PAF was dependent on the type of secretagogue. The enhancement was nearly maximal when PAF and FMP were added simultaneously to the leucocytes, whereas a preincubation of 20 min with PAF was required to get maximal enhancement with anti-IgE. The enhancing activity of PAF on HR induced by both anti-IgE and FMP was reversed by washing the cells after preincubation. While PAF enhancement of FMP-induced HR persisted on mononuclear cell fraction containing basophils, that of anti-IgE-induced HR was considerably reduced under these conditions.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailPorc et reduction des rejets azotes.
Leterme, Pascal; Thewis, André ULg

in Agricontact (1994), (259), 3-4

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See detailThe Perturbed Proximal Point Algorithm and Some of its Applications
Tossings, Patricia ULg

in Applied Mathematics & Optimization (1994), 29(2), 125-159

Following the works of R.T. Rockafellar, to search for a zero of a maximal monotone operator, and of B. Lemaire, to solve convex optimization problems, we present a perturbed version of the proximal point ... [more ▼]

Following the works of R.T. Rockafellar, to search for a zero of a maximal monotone operator, and of B. Lemaire, to solve convex optimization problems, we present a perturbed version of the proximal point algorithm. We apply this new algorithm to convex optimization and to variational inclusions or, more particularly, to variational inequalities. [less ▲]

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See detailAromatase-Immunoreactive Cells in the Quail Brain: Effects of Testosterone and Sex Dimorphism
Foidart, Agnès ULg; de Clerck, A.; Harada, N. et al

in Physiology & Behavior (1994), 55(3), 453-64

We previously demonstrated that testosterone (T) increases aromatase activity (AA) and that AA is sexually dimorphic (males > females) in the quail preoptic area (POA). The precise anatomical localization ... [more ▼]

We previously demonstrated that testosterone (T) increases aromatase activity (AA) and that AA is sexually dimorphic (males > females) in the quail preoptic area (POA). The precise anatomical localization of these effects is, however, impossible to obtain by biochemical assays even when samples are dissected by the Palkovits punch technique. We were recently able to set up an immunocytochemical (ICC) procedure that permits visualization of aromatase-immunoreactive (ARO-ir) cells in the quail brain. This showed that the ARO-ir cells of the quail POA actually outline the sexually dimorphic medial preoptic nucleus (POM). This ICC technique was used here to analyze the sex dimorphism of the quail preoptic aromatase and the localization of T effects on ARO-ir cells. In Experiment 1, the number of ARO-ir cells was counted in one section every 100 microns throughout the rostral to caudal extent of the POM of castrated birds that had been treated with increasing doses of T (5, 10, or 20 mm long Silastic implants). These T-treatments produced a dose-related increase in the sexual behavior of the birds and they increased the number of ARO-ir cells in POM, in the septal regions, and in the bed nucleus of the stria terminalis (BNST). The effect had a particularly large amplitude in the part of the POM located under the anterior commissure (AC). In Experiment 2, the same procedure was used to reanalyze the sex difference of the preoptic aromatase system. This showed that the POM of adult males contains more stained cells than the POM of females but only in a restricted region located just under and rostral to the AC. No significant sex difference was observed in the septum or in the BNST. In Experiment 3, the number of ARO-ir cells was determined in the POM of males and females that had been gonadectomized and treated with a same dose of T (40 mm implants). No sex difference in the number of ARO-ir cells could be detected in these conditions. This suggests that the sex difference in AA that had been previously observed in T-treated birds results either from a difference in aromatase concentration or activity in a similar number of positive cells or from a difference in the number of ARO-ir cells that is very discrete from the anatomical point of view.(ABSTRACT TRUNCATED AT 400 WORDS) [less ▲]

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See detailL'associatif au carrefour des économies formelle et informelle
Defourny, Jacques ULg

in Recherches Sociologiques et Anthropologiques (1994), (1994/3), 109-127

Depuis toujours, la vie associative s’est exprimée à la fois dans des formes officiellement admises par les gouvernants et dans des cadres informels, souvent même clandestins. La reconnaissance du statut ... [more ▼]

Depuis toujours, la vie associative s’est exprimée à la fois dans des formes officiellement admises par les gouvernants et dans des cadres informels, souvent même clandestins. La reconnaissance du statut juridique d’association sans but lucratif (A.S.B.L.) en 1921 a ouvert la voie à un développement considérable des structures associatives en Belgique, mais une part substantielle du phénomème associatif continue à se manifester en dehors de ce statut. Quant au bénévolat, très important dans de nombreuses associations, il renforce encore l’ancrage de l’associatif dans l’économie informelle. La coexistence de dimensions formelles et informelles ainsi que la pauvreté des statistiques officielles expliquent pour une bonne part le peu d’intérêt des économistes pour les associations. Ces dernières années cependant, l’analyse économique des associations a progressé et quelques enquêtes sont venues compléter les élémentaires recensements officiels. L’inventaire des données actuellement disponibles en Belgique montre toutefois l’ampleur du chemin qui reste à parcourir. [less ▲]

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See detailL'invasion cancéreuse mammaire: le rôle clé des cellules normales des tissus hôtes
Foidart, Jean-Michel ULg; Polette, M.; Birembaut, P. et al

in Bulletin de l'Académie Nationale de Médecine (1994), 178(3), 533-42542-4

Tumor progression is influenced by extracellular matrices and by soluble factors or cytokines locally produced by host tissue cells (fibroblasts, immune cells ...). Such factors may also accumulate in ... [more ▼]

Tumor progression is influenced by extracellular matrices and by soluble factors or cytokines locally produced by host tissue cells (fibroblasts, immune cells ...). Such factors may also accumulate in close association with some extracellular matrix molecules in the tumor. They may also be unmasked during breaking down of extracellular matrices. The most insidious aspect of tumors is their propensity to locally invade normal tissues of the host and to form secondary foci in organs at distant sites from the primary tumor called metastases. During this process, invasive cells come into contact with host tissue cells such as fibroblasts, endothelial cells, macrophages, lymphocytes. These cells are not the passive witnesses of the metastatic cascade but actively participate to the malignant invasion. Through soluble messages (cytokines) and through insoluble molecules of the extracellular matrix, neoplastic and normal cells mutually modulate their activities. Cancer cells regulate the biosynthetic activities of fibroblasts and alter in this way the scaffold of the tumor. Reciprocally, host cells secrete extracellular matrix proteins and cytokines which influence the growth and activities of tumor cells. They also produce at the periphery of tumor cells proteolytic enzymes which promote host tissue destruction and cancerous cells migration. Among these enzymes, matrix metalloproteinases appear to play a key role during invasion and metastasis. Tumors represent thus a complex ecosystem. Tumor cells interact with several components of the extracellular matrix and with host cells (immune cells, fibroblasts, endothelial cells). Such multiple cell-cell and cell-matrix interactions condition angiogenesis, tumor growth, destruction of host tissues, local migration of cancer cells and their metastatic dissemination. It is probable that a precise knowledge of the genes which are selectively activated in tumors under the influence of the host cells or of the tumor cells will allow to define new therapeutic strategies. These treatments will aim not at destroying the metastatic neoplastic cells but at preventing their growth by interfering with their microenvironment. [less ▲]

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See detailGonadotropin Releasing Hormone Inhibitory Autofeedback by Subproducts Antagonist at N-Methyl-D-Aspartate Receptors: A Model of Autocrine Regulation of Peptide Secretion
Bourguignon, Jean-Pierre ULg; Alvarez Gonzalez, Maria-Luz ULg; Gerard, Arlette ULg et al

in Endocrinology (1994), 134(3), 1589-92

The secretion of Gonadotropin-releasing Hormone (GnRH) involves activation of N-methyl-D-aspartate (NMDA) receptors. Here, we show that pulsatile GnRH secretion from hypothalamic explants is suppressed by ... [more ▼]

The secretion of Gonadotropin-releasing Hormone (GnRH) involves activation of N-methyl-D-aspartate (NMDA) receptors. Here, we show that pulsatile GnRH secretion from hypothalamic explants is suppressed by 1-5GnRH, an endogenous breakdown product of GnRH, while 2-10GnRH has no effect. GnRH secretion evoked by NMDA is selectively inhibited by 1-5GnRH and this effect is similar to that of AP-5, a competitive antagonist at NMDA receptors. In addition, 1-5GnRH accounts for a dose-related inhibition of tritiated glutamate binding to hypothalamic membrane preparations. Using GnRH secretion as a model of NMDA-receptor controlled system, the effect of different peptides has been studied. Growth Hormone Releasing Factor (GRF), Insulin-like Growth Factor-I (IGF-I) and Proinsulin result in inhibition of GnRH secretion. Bioactive subproducts of those peptides (1-29GRF, 4-701GF-I and insulin) do not show any effect, suggesting that their classical receptors are not involved. In contrast, GnRH secretion is inhibited by other subproducts (1-37GRF, 1-31GF-I and C-peptide) all terminating in a glutamate residue. These subproducts selectively suppress the NMDA-evoked secretion of GnRH. Protease inhibitors prevent the inhibitory effects of IGF-I on GnRH secretion. This, breakdown products of different peptide hormones are possible endogenous antagonists at NMDA receptors. This effect could account for an autocrine or paracrine limitation of NMDA-receptor-mediated secretion of peptides. [less ▲]

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See detailImmunocytochemical localization of vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the bovine ovary
Hulshof, S. C. J.; Dijkstra, G.; Van der Beek, E. M. et al

in Biology of Reproduction (1994), 50(3), 553-560

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty ... [more ▼]

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty, and from adult cows at three stages of the estrous cycle. The appearance of VIP and NPY immunoreactivity of 4.5-6 mo of gestation coincided with the onset of follicular development. In contrast to NPY, VIP was first found in the cortex. Both VIP and NPY immunoreactivity increased with age. From 9 mo of gestation onwards, VIP and NPY were found around blood vessels and non-vascular smooth muscle cells, in the stroma near preantral follicles, and in the theca externa of antral follicles. In addition, VIP-positive cells were observed exclusively in the granulosa layer of the preovulatory follicle at the time of the LH surge. The distribution of VIP- and NPY-immunoreactive fibers in the ovary may point to an effect of these neuropeptides on various physiological processes, including follicle development and ovarian blood flow. In addition, the presence of VIP-positive cells in the granulosa layer of the preovulatory follicle is indicative of a role for VIP in ovulation. [less ▲]

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See detailQuenching of Merocyanine 540 Triplet State by Nitroxyl Radicals in Liposomal Systems: A Laser Flash Photolysis Study
Hoebeke, Maryse ULg; Enescu, M.; Lindqvist, L.

in Journal of Photochemistry and Photobiology B : Biology (1994), 22(3), 229-33

Laser flash photolysis experiments were undertaken to investigate the interaction between stearic acid nitroxide spin probes and photoexcited merocyanine 540 (MC540) in dimyristoyl-L-alpha ... [more ▼]

Laser flash photolysis experiments were undertaken to investigate the interaction between stearic acid nitroxide spin probes and photoexcited merocyanine 540 (MC540) in dimyristoyl-L-alpha-phosphatidylcholine liposomes (membrane model). The measurements of the paramagnetic signal decay kinetics of four different spin-labelled stearic acids (n-DSA) show that the direct interaction between the dye and the probe is affected by the position of the nitroxyl group along the carbon chain. Laser flash photolysis results reveal a significant decrease in the MC540 triplet lifetime in the presence of n-DSA, the effect depending on the depth at which the nitroxyl moiety is localized in the bilayer. Previous results on the rate of disappearance of the electron spin resonance (ESR) nitroxide signal on continuous photolysis of the same systems do not show the same dependence on the localization of the nitroxyl moiety in the liposome. Although the MC540 triplet state seems to be implicated in the reaction process, the results suggest that ESR and laser flash experiments demonstrate two different kinds of mechanism. [less ▲]

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See detailIn Vivo 31p Nuclear Magnetic Resonance Spectroscopy of Skeletal Muscle Energetics in Endotoxemic Rats: A Prospective, Randomized Study
Gilles, R. J.; D'Orio, Vincenzo ULg; Ciancabilla, F. et al

in Critical Care Medicine (1994), 22(3), 499-505

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal ... [more ▼]

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal muscle energetics in endotoxemic and in control rats, by in vivo 31P nuclear magnetic resonance (NMR) spectroscopy at rest, under regional ischemia, and during reperfusion. SETTING: Biochemical NMR laboratory equipped for surgery and hemodynamic monitoring. SUBJECTS: Wistar rats were randomized to different groups. Eight rats were injected with Escherichia coli endotoxin (15 mg/kg, survival time 19 +/- 4 hrs) intraperitoneally. Seven other rats served as controls. The additional nine rats were studied for the saturation recovery pulse sequence. INTERVENTIONS: In the treatment group, endotoxin was injected 8 hrs before NMR spectroscopy. The right hind limbs were studied under anaesthesia using a surface coil NMR probe. Their high-energy phosphate contents and intracellular pH were determined by 31P NMR spectroscopy. After baseline measurements, an ischemia-reperfusion challenge was imposed on the muscle by transient clamping of the abdominal aorta. Contralateral femoral artery pressure was constantly monitored. MEASUREMENTS AND MAIN RESULTS: During the baseline period, the endotoxin-treated muscles did not show any difference in the distribution of the high-energy phosphate compounds or in intracellular pH, as compared with the controls. Ischemia resulted in a significantly faster decline of the inorganic phosphate/creatine phosphate ratio in the endotoxin-treated rats (1.35 +/- 0.17 vs. 0.51 +/- 0.06 at the end of the 38-min ischemic period). Skeletal muscle acidosis developed earlier and was deeper in the endotoxemic animals (pH: 6.94 +/- 0.02 vs. 7.02 +/- 0.03 at the end of ischemia). During reperfusion, the calculated time constants of recovery of inorganic phosphate to phosphocreatine ratios were identical between groups. CONCLUSIONS: Resting nonischemic muscles of endotoxin-treated rats show no evidence of alterations in high-energy phosphate metabolism. However, under ischemic conditions, high-energy phosphate metabolism deteriorates faster in the skeletal muscles of treated animals. These data support the hypothesis of a greater mismatch during perfusion at very low pressure between residual oxygen availability and oxygen needs in the endotoxin-treated muscle cell. [less ▲]

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See detailSalt-free polyelectrolyte behavior of polystyrene-based telechelic ionomers in a polar solvent. 1. Viscosity and low-angle light scattering studies
Wu, Jhi-Li; Wang, Yu-Min; Hara, Masanori et al

in Macromolecules (1994), 27(5), 1195-1200

Some characteristic behavior, typically observed for salt-free polyelectrolytes in water, was observed for the first time for polystyrene (PS)-based telechelic ionomers, which have ionic groups located ... [more ▼]

Some characteristic behavior, typically observed for salt-free polyelectrolytes in water, was observed for the first time for polystyrene (PS)-based telechelic ionomers, which have ionic groups located only at the chain ends, when dissolved in a polar solvent, dimethylformamide (DMF). The characteristic behavior observed by both viscosity and low-angle light scattering measurements for the telechelic ionomers, especially that of the monofunctional ionomer, demonstrates an important role of intermolecular electrostatic interactions in causing the characteristic behavior of telechelic ionomers. The behavior observed in our experiments can best be described by the following picture: each ionomer molecule with a coil conformation, very close to that of polystyrene of the same molecular weight, is separated from yet strongly interacts with other ionomer molecules; and these strong intermolecular electrostatic interactions cause a marked upturn in reduced viscosity and a reduction in scattered intensity. [less ▲]

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See detailEngineering and Overexpression of Periplasmic Forms of the Penicillin-Binding Protein 3 of Escherichia Coli
Fraipont, Claudine ULg; Adam, Maggy; Nguyen-Disteche, Martine et al

in Biochemical Journal (1994), 298(1), 189-195

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of ... [more ▼]

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of F37-V577 PBP3 and G57-V577 PBP3 respectively into the periplasm. The modified ftsI genes were placed under the control of the fused lpp promoter and lac promoter/operator; expression of the truncated PBP3s was optimized by varying the copy number of the recombinant plasmids and the amount of LacI repressor, and export was facilitated by increasing the SecB content of the producing strain. The periplasmic PBP3s (yield 8 mg/l of culture) were purified to 70% protein homogeneity. They require the presence of 0.25 M NaCl to remain soluble. Like the membrane-bound PBP3, they undergo processing by elimination of the C-terminal decapeptide I578-S588, they bind penicillin in a 1:1 molar ratio and they catalyse hydrolysis and aminolysis of acyclic thioesters that are analogues of penicillin. The membrane-anchor-free PBP3s have ragged N-termini. The G57-V577 PBP3, however, is less prone to proteolytic degradation than the F37-V577 PBP3. [less ▲]

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See detailPyridobenzoxazepine and Pyridobenzothiazepine Derivatives as Potential Central Nervous System Agents: Synthesis and Neurochemical Study
Liégeois, Jean-François ULg; Rogister, F. A.; Bruhwyler, J. et al

in Journal of Medicinal Chemistry (1994), 37(4), 519-25

In order to characterize the pharmacological profile of the different chemical classes of pyridobenzazepine derivatives, a series of N-methylpiperazinopyrido[1,4]- and -[1,5]- benzoxa- and benzothiazepine ... [more ▼]

In order to characterize the pharmacological profile of the different chemical classes of pyridobenzazepine derivatives, a series of N-methylpiperazinopyrido[1,4]- and -[1,5]- benzoxa- and benzothiazepine derivatives were prepared. The affinities for D2, D1, 5-HT2, and cholinergic (M) receptors were measured. In comparison to dibenzazepine reference compounds, a strong decrease of the affinities was observed, less pronounced, however, for the substituted analogues. Oxazepine and thiazepine analogues like clozapine (except 8-chloro-6-(4-methylpiperazin-1-yl)-pyrido[2,3-b][1,4]benzoxazepin e (9) and 8-chloro-6-(4-methylpiperazin-1-yl)pyrido[2,3-b][1,4]- benzothiazepine (11)) were found to be inactive against apomorphine stereotypies. In the open-field test in rats, different molecules showed a high disinhibitory activity as observed with anxiolytic drugs. Moreover, 8-chloro-5-(4-methylpiperazin-1-yl)pyrido[2,3-b][1,5]benzoxazepine (14) presented a clozapine-like profile that was confirmed in the behavioral model in dogs and showed most of the behavioral characteristics described for antipsychotic drugs. Its neurochemical profile, in particular the 5-HT2/D2 ratio, was also compatible with atypical antipsychotic activity. [less ▲]

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See detailFIELD OBSERVATIONS ON A MATING ATTEMPT OF A SPAWNING GRAYLING, THYMALLUS-THYMALLUS WITH A FEEDING BARBEL, BARBUS-BARBUS
Poncin, Pascal ULg

in Journal of Fish Biology (1994), 45(5), 904-906

During the spawning period of the grayling, one male was sexually stimulated by a feeding barbel and consequently exhibited the behavioural pattern of 'approach', 'quivering' and 'dorsal fin clasping'.

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See detailCoordinate Enhancement of Gelatinase a Mrna and Activity Levels in Human Fibroblasts in Response to Breast-Adenocarcinoma Cells
Noël, Agnès ULg; Polette, M.; Lewalle, J. M. et al

in International Journal of Cancer = Journal International du Cancer (1994), 56(3), 331-6

Gelatinases/type-IV collagenases are metalloproteinases involved in some carcinoma invasion and metastatic processes. The exact cellular source of the 72-kDa gelatinase A is controversial. We have ... [more ▼]

Gelatinases/type-IV collagenases are metalloproteinases involved in some carcinoma invasion and metastatic processes. The exact cellular source of the 72-kDa gelatinase A is controversial. We have analyzed the expression of mRNA coding for gelatinase A in vivo by in situ hybridization on breast-cancer tissues. The mRNA for gelatinase A was present in fibroblasts. We have therefore evaluated the gelatinase-A activity in vitro, in co-cultures of different breast adenocarcinoma cell lines and human fibroblasts. In monoculture, none of the tumor cells tested produced detectable amounts of gelatinase A. The gelatinase-A activity was enhanced in cultures of fibroblasts maintained in the presence of MDA-MB 231 or SKBR3 cells, or their conditioned medium. This increased enzymatic activity was evidenced both in the culture medium and in the membrane fraction and was paralleled by enhancement of the steady-state levels of mRNA. These results are an in vitro demonstration of a regulation of fibroblasts gelatinase-A production by soluble factors secreted by breast-tumor cells. [less ▲]

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See detailEffects of Alpha-Hederin, a Saponin Extracted from Hedera Helix, on Cells Cultured in Vitro
Danloy, S.; Quetin-Leclercq, J.; Coucke, P. et al

in Planta Medica (1994), 60(1), 45-9

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in ... [more ▼]

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in vitro. Our results indicate that, in a serum-free medium, alpha-hederin is cytotoxic and inhibits proliferation in both cell lines at rather low concentrations (< 5 micrograms/ml) after only 8 hours of treatment. Its cytotoxicity decreases in the presence of serum in which BSA seems to be able to bind the saponin. alpha-Hederin also induces vacuolization of the cytoplasm and membrane alterations leading to cell death. [less ▲]

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See detailSelf-Treatment of Acute Migraine with Subcutaneous Sumatriptan Using an Auto-Injector Device: Comparison with Customary Treatment in an Open, Longitudinal Study
Schoenen, Jean ULg; Bulcke, J.; Caekebeke, Jo et al

in Cephalalgia : An International Journal of Headache (1994), 14(1), 55-63

In a multicenter open longitudinal clinical trial where 479 patients suffering from migraine with or without aura were recruited, patients treated at home one to three migraine attacks with their ... [more ▼]

In a multicenter open longitudinal clinical trial where 479 patients suffering from migraine with or without aura were recruited, patients treated at home one to three migraine attacks with their customary treatment, and subsequently, over a 3-month period, one to three migraine attacks with 6 mg sumatriptan sc using an autoinjector. The headache response to customary treatment was 19% at 1 h and 30.5% at 2 h, and was not significantly different when only attacks treated "adequately" according to accepted treatment recommendations were considered: 16% at 1 h and 35% at 2 h. In contrast, 69% and 82% of patients treated with 6 mg sumatriptan sc had mild headache or no headache at 1 and 2 h respectively, regardless of migraine type or duration of symptoms prior to treatment. Other migraine symptoms (nausea, vomiting, photo- and phonophobia) were effectively treated with sumatriptan. Recurrence of migraine was observed in 31% of patients and was well controlled by a second injection of sumatriptan. It is concluded that 6 mg sumatriptan sc, self-administered using an autoinjector, is well tolerated and more effective than most currently used acute treatments for migraine in a population of severely affected patients consulting a neurologist. [less ▲]

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See detailLes traitements physiques des eaux dures.
Marcoen, Jean Marie ULg; Lédion, J.

in Tribune de l'Eau (La) (1994), 47

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See detailCalcul de l'équilibre calcocarbonique des eaux sur calculatrice de poche.
Wuidar, A.; Marcoen, Jean Marie ULg

in Tribune de l'Eau (La) (1994), 47(1), 11-13

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See detailHomeobox Genes: Potential Candidates for the Transcriptional Control of the Transformed and Invasive Phenotype
Castronovo, Vincenzo ULg; Kusaka, Masami; Chariot, Alain ULg et al

in Biochemical Pharmacology (1994), 47(1), 137-43

The transformation of a cell and the acquisition of the invasive and metastatic phenotype result from the activation of a group of complex cellular processes rather than from the effect of a single gene ... [more ▼]

The transformation of a cell and the acquisition of the invasive and metastatic phenotype result from the activation of a group of complex cellular processes rather than from the effect of a single gene product. It is likely that the coordination of the multiple genes involved in malignancy is under the control of a few genes that act as master genes or orchestrator genes. The latter probably code for transcription factors that control the genetic program for tumor invasion and metastasis. Homeobox genes are a family of transcription factors that contain a 183 bp highly conserved nucleotide sequence coding for a 61 amino acid domain that binds specifically to DNA. First discovered in Drosophila as genes controlling segmentation and segment identity, homeobox genes have since been identified in many other species including nematodes, frog, mouse and human. There is strong support for the suggestion that homeobox genes play a key role in development and differentiation. In humans, there are 38 homeobox genes organized in four clusters that are localized on chromosomes 2, 7, 12 and 17. The specific functions of each of these genes are generally unknown. Alterations in expression of several homeobox genes have been reported in a variety of malignant lesions, suggesting that they could play a role in the development of cancer. Using reverse transcriptase reaction coupled with polymerase chain reaction and degenerate oligonucleotides corresponding to the 5' and 3' ends of the highly conserved homeodomain, we amplified 130 bp cDNA fragments from the human breast cancer cell line MCF7 that were subsequently cloned into pBluescript vector. Sequencing of the clones, resulted in the identification of the homeodomains of four different human homeobox genes: HOXB6, HOXA1, HOXA10 and HOXC6. Further studies should determine the specific role of these four homeobox genes in the development and progression of human breast cancer and potentially determine if they might be good targets for gene therapy. [less ▲]

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See detailThe Nf-Kappa B Transcription Factor and Cancer: High Expression of Nf-Kappa B- and I Kappa B-Related Proteins in Tumor Cell Lines
Bours, Vincent ULg; Dejardin, Emmanuel ULg; Goujon-Letawe, F. et al

in Biochemical Pharmacology (1994), 47(1), 145-9

NF-kappa B is a pleiotropic transcription factor which controls the expression of many genes and viruses. To date, there is good evidence, but no definitive proof, for its role in tumor formation and ... [more ▼]

NF-kappa B is a pleiotropic transcription factor which controls the expression of many genes and viruses. To date, there is good evidence, but no definitive proof, for its role in tumor formation and development of metastasis. To investigate the possibility that members of the NF-kappa B family could participate in the molecular control of the transformed and invasive phenotype, we examined the expression of these proteins in a variety of human tumor cell lines. The expression of p50, p65, p52 and I kappa B was quantified at the protein level using western immunoblot and mobility shift assay and at the RNA level by northern blot. We observed high expression of the NF-kappa B inhibitor I kappa B in the ovarian carcinoma cell line OVCAR-3 together with constitutive nuclear NF-kappa B activity. We also studied the colon carcinoma cell line HT-29 and its metastatic counterpart HTM-29 and we observed specific expression of the p52 NF-kappa B-related protein in the metastatic cells. Our data confirm that NF-kappa B could be involved in the genesis of a variety of cancers including solid tumors and provide us with interesting models to explore the exact role of these transcription factors in cancer. [less ▲]

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See detailFurther observational evidence that MG J0414+0534 is a gravitational mirage
Angonin-Willaime, M.-C.; Vanderriest, C.; Hammer, F. et al

in Astronomy and Astrophysics (1994), 281

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the ... [more ▼]

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the (extremely red and almost featureless) spectra of the 2 brightest components are very similar. These are strong indications that the system results from multiple gravitational imaging of a single source, but the nature of this source is not yet clear. It could be the nucleus of a low metallicity galaxy at a high redshift or a new type of object. Significant differences are observed between the flux ratios of the images at radio and optical wavelenghts. The most likely explanation for this effect is a differential amplification of the image pair A[SUB]1[/SUB]-A[SUB]2[/SUB] because of the large magnification gradient near a caustic. [less ▲]

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See detailEvidence for violent ejection of nebulae from massive stars
Hutsemekers, Damien ULg

in Astronomy and Astrophysics (1994), 281(Letters), 81-84

We report the results of a systematic search for nebulae around Luminous Blue Variable LBVs) and the discovery of a strong correlation between the mass of the nebulae and the luminosity of the central ... [more ▼]

We report the results of a systematic search for nebulae around Luminous Blue Variable LBVs) and the discovery of a strong correlation between the mass of the nebulae and the luminosity of the central stars. This correlation holds for both the dust and ionized gas masses of the nebulae. The existence of a 'nebular mass-stellar luminosity' relation and the fact that not all LBVs are presently associated with a nebula give evidence against a continuous mass-loss mechanism for the formation of these nebulae. Further, the good agreement found between the observed relation and predictions by Maeder (1989), suggests that all these nebulae may be due to a violent ejection of matter caused by an instability of structural origin. [less ▲]

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See detailHypnose, hypnosédation. Conceptions actuelles et leurs applications en chirurgie plastique
Faymonville, Marie-Elisabeth ULg; Fissette, Jean ULg; Mambourg, P. H. et al

in Revue Médicale de Liège (1994), 49(1), 13-22

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See detailLe pied diabétique: etiopathogénie, prévention et traitement
Van Damme, Hendrik ULg; Paquet, Philippe ULg; Maertens de Noordhout, B. et al

in Revue Médicale de Liège (1994), 49(1), 1-13

-Le pied diabétique est la conséquence des altérations dégénératives du système vasculonerveux observées dans un diabète de longue durée. La neuropathie diabétique est le facteur essentiel dans la plupart ... [more ▼]

-Le pied diabétique est la conséquence des altérations dégénératives du système vasculonerveux observées dans un diabète de longue durée. La neuropathie diabétique est le facteur essentiel dans la plupart des cas, responsable d'hypoalgésies, microtraumatismes et ulcérations, déformation du pied (amyotrophie), et d'une eutosympethectomie (peau sèche, fissurée). Une macroangiopathie (médiacalcinose, occlusions artérielles périphériques) n'est retrouvée que dans 30 % des cas. Une microangiopathie compromet la trophicité des tissus et ralentit leur cicatrisation. Enfin, tout diabétique présente une susceptibilité élevée aux infections. Cette multitude de facteurs en cause impose des mesures de prévention. Un équilibre du profil glycémique retardera les atteintes vasculo-nerveuses. L'hygiène du pied consistera en bains de pieds, soins d'ongles et d'hyperkératoses, chaussures adaptées. En cas de troubles trophiques, une décharge d'appui sers nécessaire. Une désinfection rigoureuse, associée à une antibiothérapie (après prélèvement, si possible) aidera à éviter l'évolution vers l'abcès profond. La moindre collection sera drainée, après excision large des tissus nécrotiques. Les nécroses sèches (talons, orteils) traduisent souvent une artériopethie, pour laquelle un geste de revascularisation (protondoolestie; pontage distal) pourra être pris en considération. Parfois, l'état septique du patient, ou l'étendue de la gangrène, imposera une amputation. L'approche du pied diabétique doit toujours être multidisciplinaire (diabétologue, dermatologue, orthésiste, orthopédiste, chirurgien vasculaire), et doit commencer par des mesures préventives. [less ▲]

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See detailTransplantations de tumeurs malignes insoupçonnées lors de greffes d'organes
Detry, Olivier ULg; Detroz, Bernard ULg; D'Silva, M. et al

in Revue Médicale de Liège (1994), 49(1), 23-31

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See detailPhotometric variations of Ap stars
Manfroid, Jean ULg; Renson, Pierre ULg

in Astronomy and Astrophysics (1994), 281

The photometric variations of seven Ap stars have been analyzed from observations mainly obtained in the framework of the Long-Term Photometry of Variables project at European Southern Observatory (ESO ... [more ▼]

The photometric variations of seven Ap stars have been analyzed from observations mainly obtained in the framework of the Long-Term Photometry of Variables project at European Southern Observatory (ESO). New values for the periods and detailed light curves in the uvby system are provided. Alpha Scl is shown to display anomalous variations that set it apart from the other stars. [less ▲]

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See detailEffects of Acute Injection of Methylprednisolone in Man on Immunological and Non-Immunological Histamine Release from Leucocytes and Its Potentiation by Interleukin-3
Louis, Renaud ULg; Bury, Thierry ULg; Corhay, Jean-Louis ULg et al

in Clinical & Experimental Allergy : Journal of the British Society for Allergy & Clinical Immunology (1994), 24(1), 60-5

We investigated the effects of intravenous injection of methylprednisolone (MPR) compared with placebo (saline) on ex vivo leucocytic histamine release in eight healthy volunteers. All subjects received ... [more ▼]

We investigated the effects of intravenous injection of methylprednisolone (MPR) compared with placebo (saline) on ex vivo leucocytic histamine release in eight healthy volunteers. All subjects received in a randomized and a single-blind manner the placebo and MPR, 20 mg and 125 mg, each injection given in 2 week intervals. On each occasion blood samples were taken just before and 24 h after the intravenous injection to determine circulating leucocyte counts and leucocytic histamine release induced by anti-IgE (1/2000) and FMP (Formyl-Methionyl-Phenylalanine) (10(-5) M) and its modulation by IL-3 (2 ng/ml). MPR 20 mg and 125 mg significantly increased circulating leucocyte counts (P < 0.05 and P < 0.001 respectively) but decreased leucocytic histamine content (P < 0.05 and P < 0.001 respectively) by 24 h. Placebo had no effect. As for circulating basophils, after 24 h they were decreased by 125 mg MPR (P < 0.05) but increased by 20 mg (P < 0.05). Anti-IgE-induced HR was significantly inhibited by 125 mg MPR (P < 0.05) but not by 20 mg MPR or by the placebo. In contrast, neither MPR (20 mg and 125 mg) nor placebo significantly reduced FMP-induced HR. The strong potentiation by IL-3 of HR evoked by anti-IgE and FMP at baseline (P < 0.001) persisted 24 h after injection of MPR or placebo (P < 0.001 except P < 0.05 for anti-IgE-induced HR after 125 mg MPR).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLe point sur la production d’embryons in vitro: limitations et perspectives de la recherche
Thonon, Fabienne; Ectors, Fabien ULg; Delval, Alain et al

in Annales de Médecine Vétérinaire (1994), 138(1), 33-40

Recent progress in gamete biology and early embryo development was responsible for great evolution of reproduction techniques in farm animals. Among these techniques, in vitro production of bovine embryos ... [more ▼]

Recent progress in gamete biology and early embryo development was responsible for great evolution of reproduction techniques in farm animals. Among these techniques, in vitro production of bovine embryos was developed from slaughterhouse ovaries. This method has been used for individual animals. However, large variations in results were observed, due to several factors, including reproductive state and age of the donor. Inspite of this limitation, in vitro production of bovine embryos may be helpful, when slaughtering of valuable cows is recommended eg for brucellosis disease. Researches are purchased in order to increase the number of oocytes obtained per "elite" cow and to improve the ability of in vitro embryos to support freezing. [less ▲]

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See detailLong-Term (3 Years) Prevention of Trabecular Postmenopausal Bone Loss with Low-Dose Intermittent Nasal Salmon Calcitonin
Reginster, Jean-Yves ULg; Denis, D.; Deroisy, Rita ULg et al

in Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research (1994), 9(1), 69-73

The long-term effect of intermittent low-dose nasal salmon calcitonin on trabecular early postmenopausal bone loss was assessed as follow-up to a previously published study. Randomized controlled group ... [more ▼]

The long-term effect of intermittent low-dose nasal salmon calcitonin on trabecular early postmenopausal bone loss was assessed as follow-up to a previously published study. Randomized controlled group comparison was made of 287 healthy women with 6-36 months of natural menopause and no treatment interfering with calcium metabolism at an outpatient clinic for research in bone and cartilage metabolism. The 287 women were randomly allocated to 3 years of treatment with either 500 mg/day, 5 days/week of calcium or the same amount of calcium plus 50 IU/day, 5 days per week of nasal salmon calcitonin. A total of 186 women complied with the study protocol throughout. The main outcome measures were bone mineral density of the lumbar spine (DPA) and biochemical parameters reflecting bone turnover (serum alkaline phosphatases, urinary calcium/creatinine, and hydroxyproline/creatinine ratio). The average changes in bone mineral density after 36 months showed a positive (p < 0.05) outcome (1.8 +/- 5.7%; mean +/- SD) in the group treated with salmon calcitonin and calcium and a significant (p < 0.01) loss (-5.8 +/- 4.8%) in patients receiving calcium alone. The difference between the evolution of the two groups was significantly (p < 0.01) different after 6 months of treatment and remained so until the end of the study. No significant changes were recorded in biochemical parameters reflecting bone turnover. As previously shown during a 1 year follow-up, nasal salmon calcitonin given at low dose and intermittently, in association with calcium, can counteract trabecular postmenopausal bone loss. [less ▲]

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See detailLa tribologie, une spécialité liégeoise
Bozet, Jean-Luc ULg

in Athena (1994), 97

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See detailAnaphylatoxin C5a Production During Short-Term Submaximal Dynamic Exercise in Man
Camus, G.; Duchateau, J.; Deby, Ginette ULg et al

in International Journal of Sports Medicine (1994), 15(1), 32-5

We studied the effects of short-term submaximal exercise on the plasma levels of myeloperoxidase ([MPO]) and C5a anaphylatoxin ([C5a]), taken as specific markers of polymorphonuclear neutrophil (PMN) and ... [more ▼]

We studied the effects of short-term submaximal exercise on the plasma levels of myeloperoxidase ([MPO]) and C5a anaphylatoxin ([C5a]), taken as specific markers of polymorphonuclear neutrophil (PMN) and complement activation, respectively. Eleven young, healthy male volunteers were subjected to a constant-load concentric exercise on a cycle ergometer (20 min at 80% maximal oxygen uptake). Mean resting MPO and C5a concentrations were 437 +/- 113 and 0.47 +/- 0.21 ng/ml, respectively. During exercise, [MPO] and [C5a] increased significantly (p < 0.001) towards respective peak values of 649 +/- 131 and 1.3 +/- 0.6 ng/ml. A rapid decrease of both [MPO] and [C5a] was observed during recovery. The similar time course of [MPO] and [C5a] changes and the highly significant relationship between these two variables (r = 0.651; p < 0.001) argues for the possible involvement of the complement anaphylatoxin C5a in the process of PMN degranulation. During exercise, the number of circulating PMN increased (+80%; p < 0.001) and remained practically unchanged up to 20 minutes of recovery. As [MPO] and PMN count were not significantly related (r = 0.2; p < 0.1), we concluded that the activation of PMN was independent of their mobilization. [less ▲]

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See detailEffects of Apomorphine on Sexual Behavior in Male Quail
Absil, Philippe ULg; Das, S.; Balthazart, Jacques ULg

in Pharmacology, Biochemistry & Behavior (1994), 47(1), 77-88

In the rat, dopamine (DA) facilitates male copulatory behavior. Indirect evidence based largely on neuroanatomical data suggest that in quail DA is also implicated in the control of male reproductive ... [more ▼]

In the rat, dopamine (DA) facilitates male copulatory behavior. Indirect evidence based largely on neuroanatomical data suggest that in quail DA is also implicated in the control of male reproductive behavior but there is no pharmacological evidence to support this conclusion. To test this idea, castrated testosterone (T)-treated male quail were injected with various doses of the dopaminergic agonist apomorphine (APO) in the range 1-10,000 micrograms/kg. The sexual behavior of birds was recorded starting 15 min after APO injection for a duration of 30 min. A dose-dependent inhibition of male reproductive behavior that lasted for the entire duration of the test was observed. In a second experiment, gonadectomized T-treated male Japanese quail were injected daily with APO (0, 10, or 1,000 micrograms/kg) during 8 days. Their sexual interactions with a partner were quantified either 24 h or 15 min after the last injection. No influence of the treatment on copulatory behavior was observed 24 h after the last injection, but a strong inhibition was present when the test was performed 15 min after. To research whether the inhibitory effects of APO were due to a preferential action on D2 presynaptic autoreceptors, male quail were pretreated with two different D2 antagonists (spiperone or pimozide; 0.5 or 2 mg/kg) before being injected with APO (100 micrograms or 1 mg/kg). Spiperone facilitated male sexual behavior but did not suppress the inhibitory effect of APO. No significant effect of pimozide was observed. These results support the notion that DA modulates male sexual activity in the Japanese quail. The specific role of the different dopaminergic receptor subtypes remains, however, to be elucidated. [less ▲]

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See detailDetection of Human Papillomaviruses in Paraffin-Embedded Biopsies of Cervical Intraepithelial Lesions: Analysis by Immunohistochemistry, in Situ Hybridization, and the Polymerase Chain Reaction
Delvenne, Philippe ULg; Fontaine, Marie-Anne; Delvenne, Catherine ULg et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (1994), 7(1), 113-119

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ ... [more ▼]

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (Vira-Type in situ assay; Digene Diagnostics, Silver Spring, MD) or probes labeled with digoxigenin by the random primer technique. Immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared to 61% (86/140) for the in situ assay with the digoxigenin-labeled probes. Biotinylated probes proved to be slightly less sensitive than digoxigenin-labeled probes, with a detection rate of 53% (74/140). Although less sensitive in our series taken as a whole, immunohistochemistry was positive in a few cases of CILs negative by in situ hybridization, so that the association of these techniques gave the highest detection rate (66%; 92/140). The CILs that remained negative with these methods (34%; 48/140) were investigated by the polymerase chain reaction (PCR) using consensus primers to determine definitively the presence of HPV in these lesions and were reviewed histologically to assess the diagnosis of CILs. The PCR method increased the detection rate of HPV in our series to 76% (107/140). The diagnosis of CILs was confirmed for all the biopsy specimens positive by PCR (15/15; 100%) and for all the HPV negative tissues with histological features of a high-grade lesions (7/7; 100%).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailInsulin expression in pancreatic islet cells relies on cooperative interactions between the helix loop helix factor E47 and the homeobox factor STF-1.
Peers, Bernard ULg; Sharma, S.; Teitelman, G. et al

in Molecular Endocrinology (1994), 8(12), 1798-806

The development of endocrine cell types within the pancreas is thought to involve the progressive restriction of pluripotential stem cells, which gives rise to the four major cell types: insulin ... [more ▼]

The development of endocrine cell types within the pancreas is thought to involve the progressive restriction of pluripotential stem cells, which gives rise to the four major cell types: insulin-, glucagon-, somatostatin-, and pancreatic polypeptide-expressing cells. The mechanism by which these peptide hormone genes are induced and then either maintained or repressed during development is unknown, but their coexpression in early precursor cells suggests the involvement of common regulatory factors. Here we show that the somatostatin transcription factor STF-1 is also a principal regulator of insulin expression in beta-cells of the pancreas. STF-1 stimulates the insulin gene by recognizing two well defined islet-specifying elements on the insulin promoter and by subsequently synergizing in trans with the juxtaposed helix-loop-helix protein E47. Within the STF-1 protein, an N-terminal trans-activation domain functions cooperatively with E47 to stimulate insulin transcription. As truncated STF-1 polypeptides lacking the N-terminal activation domain strongly inhibit insulin promoter activity in beta-islet cells, our results suggest that the specification of islet cell types during development may be in part determined by the expression of STF-1 relative to other islet cell factors. [less ▲]

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See detailSymbolic Verification with Periodic Sets
Boigelot, Bernard ULg; Wolper, Pierre ULg

in Lecture Notes in Computer Science (1994), 818

Symbolic approaches attack the state explosion problem by introducing implicit representations that allow the simultaneous manipulation of large sets of states. The most commonly used representation in ... [more ▼]

Symbolic approaches attack the state explosion problem by introducing implicit representations that allow the simultaneous manipulation of large sets of states. The most commonly used representation in this context is the Binary Decision Diagram (BDD). This paper takes the point of view that other structures than BDD's can be useful for representing sets of values, and that combining implicit and explicit representations can be fruitful. It introduces a representation of complex periodic sets of integer values, shows how this representation can be manipulated, and describes its application to the state-space exploration of protocols. Preliminary experimental results indicate that the method can dramatically reduce the resources required for state-space exploration. [less ▲]

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See detailVariations of lyoluminescence with commercial origin of the dosimeters
Galand, Eric; Niezette, Jacques; Vanderschuren, Jacques et al

in Radiation Physics and Chemistry (1994), 43

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See detailModélisation mathématique appliquée à la délimitation des zones de protection: cas du site de captage de Vivegnis (plaine alluviale de la Meuse, Belgique)
Derouane, Johan; Dassargues, Alain ULg

in Bulletin du Centre d'Hydrogéologie de l'Université de Neuchätel (Suisse) (1994), 13

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See detailPump up the volume! Mogelijkheid en onmogelijkheid van het manifest in postmoderne tijden
Spinoy, Erik ULg

in De Zingende Zaag (1994), (24-25), 102-123

This article reflects on the fact that manifests are a predominantly modernist genre. In postmodern times, manifests can only reappear as simulations or parodies.

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See detailAirway occlusion pressure and diaphragm global electromyograph analysis for evaluation of inspiratory muscle drive and neuromechanical coupling in cattle
Desmecht, Daniel ULg; Linden, Annick ULg; Rollin, Frédéric ULg et al

in American Journal of Veterinary Research (1994), 55

Although healthy and diseased bovine respiratory tracts have been intensively studied during the last years, to the authors' knowledge, there have been no attempts to objectively examine the inspiratory ... [more ▼]

Although healthy and diseased bovine respiratory tracts have been intensively studied during the last years, to the authors' knowledge, there have been no attempts to objectively examine the inspiratory drive from the brain to the nerves and muscles and its transformation in pressure. Such technique would be useful in assessing the possibility of altered ventilatory drive or inspiratory muscle fatigue in the context of an animal with ventilatory failure. The relation among ventilation, airway opening occlusion pressure generated 100 milliseconds after onset of inspiration (Pawo100ms) and 6 indexes describing diaphragmatic electromyographic activity (EMGdi) recorded via implanted fishhooks was evaluated during free and impeded CO2 rebreathing in 6 young bulls. The best significant linear correlations (r > 0.8) with inspiratory center afferent stimulation, as judged by end-tidal CO2 concentration in expired air, were found for Pawo100ms, peak moving time average or variance EMGdi, and mean integrated EMGdi, whatever had been the respiratory impedance. However, with an inspiratory load, Pawo100ms responses systematically had greater increase for a given change in the driving EMGdi, implying dependence of the former not only on neural input, but also on configurational factors that determine inspiratory muscle excitation-pressure generation couplings. The reproducibility of EMGdi absolute values and changes was satisfactory up to 10 hours, but could not be repeated from one day to the other. It was concluded that, provided the constancy of the electrical coupling of the recording system to the tissue being studied is ensured, specific EMGdi and Pawo100ms values correlate reliably with amount of CO2 during free and loaded breathing.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLocalisation scintigraphique de tumeurs neuroendocrines et de leurs métastases par un analogue de la somatostatine: [111In-DTPA-d-phe1] octreotide
Beckers, Albert ULg; Pham, V. T.; Abs, R. et al

in Médecine & Chirurgie Digestives (1994), 23(3), 145-150

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See detailPilgrimage out of Dispossession
Pagnoulle, Christine ULg

in Commonwealth : Essays and Studies (1994), 17(1), 100-106

Goodison's poems testify to her proud self-assertion, both as woman and as a descendant of African slaves.

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See detailL'entérotoxémie bovine en Belgique. I. Introduction et contexte bibliographique.
Manteca, C.; Daube, Georges ULg

in Annales de Médecine Vétérinaire (1994), 138

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See detailEvaluation de la fonction pulmonaire du cheval par la technique scintigraphique
Votion, Dominique ULg; Art, Tatiana ULg; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (1994), 138

Scintigraphy of the respiratory system, a technique used extensively in human medecine, provides a direct and non-invasive measure of both total and regional ventilation and perfusion of each lung. This ... [more ▼]

Scintigraphy of the respiratory system, a technique used extensively in human medecine, provides a direct and non-invasive measure of both total and regional ventilation and perfusion of each lung. This article remind you some nuclear notions and describes scintigraphical techniques for horses. The resuits of studies suggest that ventilation! perfusion scintigraphy provides valuable additionnai informations on regional lung function that are not obtainable from conventional investigations. The mucociliary clearance rates from the trachea can be determinated using scintigraphy and the differenttherapeutic drugs, which are claimed to increase mucokinesis, are successively presented. Methods of radiation protection in equine nuclear medecine are described [less ▲]

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See detailEtude épidémiologique de la toxoplasmose chez les femmes enceintes à Amiens (Picardie). Nécessité d'une enquête nationale
Carme, Bernard; Lenne, Evelyne; Tirard, Véronique et al

in Médecine et Maladies Infectieuses (1994), 24

L'étude avait pour but d'évaluer le niveau d'immunité vis à vis de la toxoplasmose des femmes venant d'accoucher à Amiens entre mars 1993 et avril 1994 ainsi que leur connnaissance des mesures préventives ... [more ▼]

L'étude avait pour but d'évaluer le niveau d'immunité vis à vis de la toxoplasmose des femmes venant d'accoucher à Amiens entre mars 1993 et avril 1994 ainsi que leur connnaissance des mesures préventives vis à vis de cette parasitose. Cette étude a permis de mettre en évidence la bonne connaissance des mesures préventives vis à vis de la toxoplasmose chez les femmes non immunes. Ceci a permis également de préciser les données épidémiologiques de la toxoplasmose à Amiens. Le choix d'un protocole incluant les femmes au moment de l'accouchement pourrait être complété par celui de femmes en début de grosesse pour éviter d'exclure les interruptions de grossesse. Cette étude devrait déboucher sur une enquête nationale qui permettrait de préciser l'épidémiolgoie de la toxoplasmose en France. [less ▲]

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See detailFunctional effects of a muscarinic receptor blockade during acute respiratory distress syndrome in double-muscled calves
Genicot, Bruno; Mouligneau, Frédéric; Close, Roland et al

in Veterinary Record : Journal of the British Veterinary Association (1994), 134(5), 110-113

Eighteen Belgian white and blue double-muscled calves suffering from the acute respiratory distress syndrome were studied. Fifteen of the calves inhaled ipratropium bromide (0.6 mg) four times a day for ... [more ▼]

Eighteen Belgian white and blue double-muscled calves suffering from the acute respiratory distress syndrome were studied. Fifteen of the calves inhaled ipratropium bromide (0.6 mg) four times a day for three to four days whereas the other three control calves inhaled sterile 0.9 per cent saline. All the animals were injected with ceftiofur sodium (1 mg/kg/day) for five days, the first injection being given one hour after the first inhalation of ipratropium bromide or saline. Arterial oxygen tension, alveolar arterial oxygen difference, carbon dioxide tension and arterial pH, respiratory and heart rates, oscillatory resistance and phase angle, measured by the mono-frequency forced oscillation technique, were recorded both before and one hour and 168 hours after the first inhalation. The measurement of oscillatory resistance and phase angle made it possible to resolve the impedance of the respiratory system into its real and imaginary components. The oscillatory compliance (Cosc) was determined from the imaginary component (Im). By one hour after the first inhalation of ipratropium bromide the oscillatory resistance was already significantly reduced and Im and Cosc had significantly increased, but the other parameters showed no significant improvement. However, between one hour and 168 hours after the first inhalation all the parameters reached physiological values. The control calves did not show any change. It was concluded that the pulmonary dysfunction associated with the acute respiratory distress syndrome in these calves was at least partly due to a severe bronchoconstriction. [less ▲]

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See detailDe réels progrès ont-ils été faits dans le traitement de l'ostéoporose ?
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Franchimont, P

in Les Nouvelles de Rhumatologie (1994), 4

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See detailL'enseignement du français instrumental à l'université: quelles possibilités?
Vanden Berghe, Kristine ULg; Sanctobin, Véronique; Slootmaekers, An et al

in Rencontres (1994), 5

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See detailDistribution and Regulation of Estrogen-2-Hydroxylase in the Quail Brain
Balthazart, Jacques ULg; Stoop, R.; Foidart, Agnès ULg et al

in Brain Research Bulletin (1994), 35(4), 339-45

The anatomical distribution and endocrine regulation of the estrogen-2-hydroxylase activity were investigated in the brain of adult male and female Japanese quail. Significant levels of enzymatic activity ... [more ▼]

The anatomical distribution and endocrine regulation of the estrogen-2-hydroxylase activity were investigated in the brain of adult male and female Japanese quail. Significant levels of enzymatic activity were detected in all brain regions that were studied, but the highest levels were observed in preoptic and hypothalamic brain nuclei that are known to contain high levels of aromatase activity. These data are consistent with previous results suggesting that the placental aromatase is also responsible for the estrogen-2-hydroxylase activity. However, there is a marked sex difference and a control by T of aromatase activity in the quail brain, and no such difference in 2-hydroxylase activity could generally be detected except in the VMN. Further studies will be needed to know whether the previously published conclusions concerning the human placenta also apply to the brain. The present data are consistent with the idea that estrogens formed locally in the brain by testosterone aromatization could affect reproduction by interfering with the catecholaminergic transmission after being metabolized into catechol-estrogens. [less ▲]

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See detailCompte-rendu de Lucie Antoniol, Lire Ryle aujourd'hui. Aux sources de la philosophie analytique
Leclercq, Bruno ULg

in Revue Philosophique de Louvain (1994), 92

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See detailMassive Ketonuria During Sedation with Propofol in a 12 Year Old Girl with Severe Head Trauma
Canivet, Jean-Luc ULg; Gustad, K.; Leclercq, P. et al

in Acta Anaesthesiologica Belgica (1994), 45(1), 19-22

Severe ketonuria developed during sedation with propofol in a 12 year old girl with brain injury. Deep sedation with propofol (5.1 mg/kg/h) was required because of agitation and severe intracranial ... [more ▼]

Severe ketonuria developed during sedation with propofol in a 12 year old girl with brain injury. Deep sedation with propofol (5.1 mg/kg/h) was required because of agitation and severe intracranial hypertension; as a part of our management protocol, glucose intake was restricted to 5 Kcal/h. After 18 hours of propofol infusion there was intense ketonuria (8+ by Ketostix) without any evidence of metabolic acidosis (pH, HCO3- and anion gap were within normal values). At this time, indirect calorimetry (Deltatrac) confirmed that energy expenditure was principally based on fat consumption (70% of energy expenditure). Lowering the propofol infusion rate and increasing glucose intake reduced fat consumption to 39% within 8 hours: at this time, Ketostix was negative for ketone bodies. This case illustrates a potential risk of ketonuria during prolonged sedation with propofol (a 10% solution of intralipid), particularly if glucose intake is restricted. Monitoring urinary ketone bodies is recommended under these circumstances. [less ▲]

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See detailVers une théorie générale du signe
Badir, Sémir ULg

in Degrés : Revue de Synthèses à Orientation Sémiologique (1994), 79-80

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See detailCompte rendu de L.G. Westerink, Stephanus of Athens. Commentary on Hippocrates'Aphorisms, Sections I-II, Berlin, 1985
Marganne, Marie-Hélène ULg

in Archives Internationales d'Histoire des Sciences (1994), 44

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See detailModification of immunoreactive EGF receptor after acute tubular necrosis induced by tobramycin or cisplatin
Leonard, I.; Zanen, J.; Nonclercq, D. et al

in Renal Failure (1994), 16(5), 583-608

Acute tubular necrosis induced by aminoglycoside antibiotics and various other nephrotoxins is followed by a regenerative process which leads to the restoration of damaged tubules. Several lines of ... [more ▼]

Acute tubular necrosis induced by aminoglycoside antibiotics and various other nephrotoxins is followed by a regenerative process which leads to the restoration of damaged tubules. Several lines of evidence indicate that tubular regeneration is mediated by polypeptide growth factors such as epidermal growth factor (EGF). Previous studies devoted to cisplatin nephrotoxicity have shown that this agent causes tubular cystic degeneration possibly related to an impairment of renal tissue repair. Thus, we examined on a comparative basis the time course of the regenerative response subsequent to tubular damage induced by tobramycin or cisplatin, particular attention being paid to renal EGF and its receptor. Female Sprague-Dawley rats (160-180 g body weight) were treated during 4 consecutive days with daily doses of 200 mg/kg tobramycin i.p. (BID) or 2 mg/kg cisplatin (once a day). Sham-treated rats were given 0.9% NaCl i.p. following the same protocol. Groups of experimental animals (n = 5-10) were terminated at increasing time intervals (1, 4, 7, 14, 21, 60 days) after cessation of treatment. One hour prior to sacrifice, each individual received i.p. 200 mg/kg 5-bromo-2'-deoxyuridine (BrdU) for the immunohistochemical demonstration of cell proliferation. Blood was collected at the time of sacrifice in order to assess glomerular filtration rate by measuring serum creatinine and BUN levels. Kidneys were analyzed with respect to total EGF determined by RIA in renal tissue homogenates, and soluble EGF was assayed in extracts prepared by centrifugation. Renal tissue was processed for the immunohistochemical detection of S-phase cells, of EGF, of EGF receptors, and of the intermediate filament vimentin, the latter being used as a marker of epithelium dedifferentiation. In absence of nephrotoxic alterations, EGF was immunolocalized in distal tubules, whereas EGF receptor immunostaining was seen in proximal tubules cells. Vimentin immunostaining was confined to glomeruli and blood vessels. Tobramycin and cisplatin caused acute tubular necrosis in proximal convoluted tubules and proximal straight tubules, respectively. Tissue damage was accompanied by renal dysfunction reflected by an elevation of serum creatinine and BUN levels. Tubular necrosis was followed by a proliferative response indicative of tubular regeneration. Regenerative hyperplasia was associated with a reduction of total immunoreactive EGF due to a decrease of tissue-bound proEGF. Tubules undergoing regenerative repair were characterized by a disappearance of EGF receptors and the presence of immunoreactive vimentin. In tobramycin-treated rats, renal dysfunction lasted for 4-7 days and was fully reversible, as indicated by the return of serum markers to normal values. [less ▲]

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See detailAspergillose chez les oiseaux marins échoués sur la côte belge
Jauniaux, Thierry ULg; Coignoul, Freddy ULg

in Annales de Médecine Vétérinaire (1994), 138(4), 277-281

Pulmonary aspergillosis is a respiratory disease usually present in birds. The agent is an ubiquitous and saprophitic fungus, belonging to the Aspergillus genus, A. fumigatus being the predominant species ... [more ▼]

Pulmonary aspergillosis is a respiratory disease usually present in birds. The agent is an ubiquitous and saprophitic fungus, belonging to the Aspergillus genus, A. fumigatus being the predominant species. Debilitation and stress are favourable for the mycosis installation in captive wild birds (Zoo and rehabilitation centers). During 90-91 and 91-92 winters, 386 birds found dead at the belgian seashore were necropsied and 9 birds were carriers of specific lesions. Complementary investigations were undertaken. Most animals were oiled and had been kept for some time in rehabilitation centers. [less ▲]

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See detailUn affranchi trop discret? : A propos des lignes 13-19 du P. Matr. Daris 2
Straus, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1994), LXIX(137), 127-131

Born of manumitted parents, Sarapion introduice himself as a freeborn though he too is a freedman. Observations on the way he got a patch of land and on the affective and sexual life of the slaves.

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See detailConséquences microcirculatoires et cellulaires de l'endotoxine
D'Orio, Vincenzo ULg

in Réanimation (1994), 1

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See detailCrise de presse. Le journalisme au péril du « reportage » (France, 1870-1890)
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1994), 24(automne), 123-152

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See detailLe Paléolithique supérieur de Moldavie. Recherches récentes
Noiret, Pierre ULg; López Bayón, Ignacio

in Bulletin de l'Association scientifique liégeoise pour la Recherche archéologique (1994), XXI

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See detailJean Capart ou la naissance de l’égyptologie belge
Winand, Jean ULg

in Egyptes (1994), 4

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See detailUtility of non-invasive ambulatory bood pressure monitoring in a hypertension clinic
Saint-Remy, Annie ULg; Dubois, Bernard ULg; Krzesinski, Jean-Marie ULg et al

in CVD Epidemiology newsletter (1994), 49(Spring), 50

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See detailPlasma Beta-Endorphin Response of Thoroughbred Horses to Maximal Exercise
Art, Tatiana ULg; Franchimont, P.; Lekeux, Pierre ULg

in Veterinary Record : Journal of the British Veterinary Association (1994), 135(21), 499-502

Ten horses underwent a standardised strenuous treadmill exercise test, before, during and after which measurements were made of plasma beta-endorphin and cortisol concentrations, blood lactate, glucose ... [more ▼]

Ten horses underwent a standardised strenuous treadmill exercise test, before, during and after which measurements were made of plasma beta-endorphin and cortisol concentrations, blood lactate, glucose, haemoglobin and pH, the activities of creatine kinase, lactate dehydrogenase and aspartate amino-transferase, and heart rate, oxygen uptake and expired minute volume. The correlations between the exercise-induced response of beta-endorphin and the changes observed in the other physiological measurements were examined. There was a large variation in the beta-endorphin response of the horses to exercise. The increase in beta-endorphin was correlated significantly and inversely with the treadmill velocity at which maximal oxygen uptake was reached. It was also significantly and directly correlated with the heart rate during recovery, the increase in plasma lactate concentration and the change in blood pH, indicating that the exercise-induced increase in beta-endorphin concentration was smaller in horses with a higher aerobic capacity [less ▲]

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See detailGreffes de moelle dans les hémoglobinopathies
Beguin, Yves ULg

in Mini Acta (1994), 26

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See detailDetection des molecules responsables de l'activite biocide des feuilles de Boscia senegalensis (Pers.) Lam ex Poir. par echantillonage d'espace de tete et spectrometrie de masse couplee a la chromatographie en phase gazeuse.
Lognay, Georges ULg; Marlier, M.; Seck, D. et al

in Bulletin des Recherches Agronomiques de Gembloux (1994), 29(1),

Des échantillons de feuilles de Boscia senegalensis (Prns.) I.ev ex Porn. ont été tnaintenus dans des conditions contrôlées sous un flux d'hélium, à l'intérieur d'une.platine de désorption thermique ... [more ▼]

Des échantillons de feuilles de Boscia senegalensis (Prns.) I.ev ex Porn. ont été tnaintenus dans des conditions contrôlées sous un flux d'hélium, à l'intérieur d'une.platine de désorption thermique directement connectée à un GC-MS. A côté du trans-2-hexénal, responsable de la note "verte" des feuilles, l'isothiocyanate de méthyle et le cyanure de méthyle (acétonitrile) ont été détectés. Ces deux produits se sont formés par dégradation enzymatique in situ d'un précurseur, la glucocapparine. Ils ont été caractérisés par leur spectre de masse. La libération de cyanure de méthyle à partir d'échantillons biologiques est expliquée. [less ▲]

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See detailLa régionalisation du droit pénal international
Flore, Daniel ULg; Thomas, F.

in Revue internationale de droit pénal (1994)

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See detailThe experience of the CHU Liege with conservative surgery in the management of upper urinary tract tumors.
Bouffioux, Christian ULg; Andrianne, Robert ULg; Bonnet, Pierre ULg et al

in Acta Urologica Belgica (1994), 62(4), 39-43

A review of 20 cases of conservative surgery for urothelial tumors of the upper tract is presented. The morbidity is limited; the recurrence rate is around 15% in the ipsilateral urinary tract with a mean ... [more ▼]

A review of 20 cases of conservative surgery for urothelial tumors of the upper tract is presented. The morbidity is limited; the recurrence rate is around 15% in the ipsilateral urinary tract with a mean follow up of 41 months. Conservative surgery does not seem to carry a higher fatal risk as compared to more radical surgery. It has indications in some cases which are defined. [less ▲]

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See detailRecension de l'ouvrage de Silvano Petrosino, "Jacques Derrida et la loi du possible"
Steinmetz, Rudy ULg

in Cahiers Internationaux de Symbolisme (1994)

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See detailTamoxifen restores the E-cadherin function in human breast cancer MCF-7/6 cells and suppresses their invasive phenotype
Bracke, M. E.; Charlier, Corinne ULg; Bruyneel, E. A. et al

in Cancer Research (1994), 54

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See detailEvaluation de la chimiorésistance de Plasmodium falciparum. Revue des tests in vitro: méthodologie, intérêts et limites
Hayette, Marie-Pierre ULg; Carme, Bernard

in Publications Médicales Africaines (1994), 129

The chloroquine-resistance of Plasmodium falciparum appeared first in Southeast Asia and South-America during the sixties. It reached further the african continent in 1977, through Kenya and Tanzania. In ... [more ▼]

The chloroquine-resistance of Plasmodium falciparum appeared first in Southeast Asia and South-America during the sixties. It reached further the african continent in 1977, through Kenya and Tanzania. In Africa, the front of the resistance spread from East to West, and now completely covers Black Africa with different degrees of resistance. Rapidly, "in vivo" and "in vitro" tests were developped to assess the sensitivity of P. falciparum strains against the different antimalarial drugs. This paper is a review of existing "in vitro" and "in vivo" tests with their interest and limits. [less ▲]

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See detailTechniques moleculaires de detection et d'identification des agents phytopathogenes.
Lepoivre, Philippe ULg; Kummert, J.; Colinet, D. et al

in Cahiers Agricultures (1994), 3(4),

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See detailChromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers
Cockett, N. E.; Jackson, S. P.; Shay, T. D. et al

in Proceedings of the National Academy of Sciences of the United States of America (1994), 91

A mutation causing muscular hypertrophy, with associated leanness and improved feed efficiency, has been recently identified in domestic sheep (Ovis aries). Preliminary results indicate that an autosomal ... [more ▼]

A mutation causing muscular hypertrophy, with associated leanness and improved feed efficiency, has been recently identified in domestic sheep (Ovis aries). Preliminary results indicate that an autosomal dominant gene may be responsible for this economically advantageous trait. We have exploited the conservation in sequence and chromosomal location of DNA markers across Bovidae to map the corresponding callipyge locus to ovine chromosome 18 using a battery of bovine chromosome 21 markers. Chromosomal localization of the ovine callipyge locus is the first step toward positional cloning of the corresponding gene. [less ▲]

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