References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailSens de la cohérence, colère et trouble obsessionnel-compulsif
Lambert, Stéphanie; Etienne, Anne-Marie ULg; Fontaine, Ovide ULg

in Revue Francophone de Clinique Comportementale et Cognitive (2001), 6(4), 5-16

This study assesses the relationship between the Sense of Coherence (SoC) of Antonovsky, anger and the obsessive-compulsive disorder. The sample is composed of 42 subjects divided in two groups, 21 ... [more ▼]

This study assesses the relationship between the Sense of Coherence (SoC) of Antonovsky, anger and the obsessive-compulsive disorder. The sample is composed of 42 subjects divided in two groups, 21 subjects suffering of obsessive-compulsive disorder and 21 subjects matched controls. They completed a demographic questionnaire, the list of obsessions and compulsions of Goodman & al.(1989), translation Mollard, Cottraux & Bouvard (1989), the Visual Analogues Scales, the Orientation Life Questionnaire of Antonovsky (1987), the State-Trait Anger Expression Inventory of Spielberger (STAXI), the State-Trait Anxiety Inventory of Spielberger (STAI), and the Beck Depression Inventory (BDI). The results show higher scores of anxiety and depression in obsessive-compulsive patients than in controls subjects. According to the results obtained with the Anger Inventory, the obsessive-compulsive subjects show higher scores of state-anger, of anger-expression and higher scores of anger-control. The results of the Sense of Coherence scale reveal a lower SoC in TOC group than in control group. The SoC is also negatively associated with depression and anxiety in both groups. The SoC is negatively associated with the control of anger in the TOC group and with the suppression of anger in the control group. The theorical and clinical implications of these results are discussed. [less ▲]

Detailed reference viewed: 351 (9 ULg)
Full Text
Peer Reviewed
See detailAttachment of “menstrual” endometrium to peritoneum?
Donnez, Jacques; NISOLLE, Michelle ULg

in Fertility and Sterility (2001), 76(3), 642

Detailed reference viewed: 34 (3 ULg)
Full Text
Peer Reviewed
See detailA self-consistent model of the Jovian auroral thermal structure
Grodent, Denis ULg; Waite, J. Hunter; Gérard, Jean-Claude ULg

in Journal of Geophysical Research (2001), 106(A7), 12933-12952

A one-dimensional (1-D) model coupling a two-stream electron transport model of energy deposition with a 1-D thermal conduction model has been developed. It is applied to investigate the links between ... [more ▼]

A one-dimensional (1-D) model coupling a two-stream electron transport model of energy deposition with a 1-D thermal conduction model has been developed. It is applied to investigate the links between auroral heat input and the vertical temperature of Jupiter's upper atmosphere. Two energy distributions meant to reproduce the emissions of a diffuse and a discrete aurora are used to evaluate the importance of the energy spectrum of the incident electrons for the thermal balance of Jupiter's auroral thermosphere. The values of observable quantities such as the altitude of the H-2 emission peak, thermal infrared (LR), ultraviolet (UV) emissions, and temperatures associated with various optical signatures are used to constrain the parameters of these distributions. It is shown that the high-energy component of these energy distributions heats a region of the homosphere between 10(-4) and 10(-6) bar and mainly controls the H-2 temperature and the far-UV (FUV) emission. A 3-keV soft electron component is necessary to heat the region directly above the homopause, between 10(-6) and 10(-9) bar. It has a large influence on the H-2 and H-3(+) temperatures and on the H-3(+) near-IR(NIR) emission. It is used in conjunction with a weak 100 eV component which is responsible for heating the thermosphere, from 10(-9) to 10(-12) bar and exerts a control on the exospheric temperature. The calculated temperatures, UV, and IR emissions suggest that the model probably misses a nonparticle heat source in the 10(-5) bar region, that is expected to balance the strong hydrocarbon cooling. Sensitivity tests are performed to evaluate the importance of the parameters of the energy distributions. They show that the FUV color ratio increases with the characteristic energy (or high-energy cutoff) of the high-energy component, while the H-2 rovibrational temperature varies inversely. A trade-off is therefore necessary for these two parameters to simultaneously meet their observational constraints. Further tests demonstrate the essential thermostatic role played by H-3(+), which regulates the net heating in the thermosphere. An increased eddy diffusion reproduces the effect of a possible auroral upwelling of methane but gives rise to an H-2 temperature smaller than the observed value. [less ▲]

Detailed reference viewed: 28 (14 ULg)
Full Text
Peer Reviewed
See detailLes bioindustries fertilisent la valorisation
Thonart, Philippe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(3), 131-134

Detailed reference viewed: 7 (2 ULg)
Full Text
Peer Reviewed
See detailPerspectives de vaccination anti-dermatophytique chez les carnivores domestiques
Descamps, F.; Brouta, F.; Losson, Bertrand ULg et al

in Annales de Médecine Vétérinaire (2001), 145

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailChanges in polyamines, auxins and peroxidase activity during in vitro rooting of Fraxinus angustifolia shoots: an auxin-independent rooting model
Tonon, Giustino; Kevers, Claire ULg; Gaspar, Thomas ULg

in Tree Physiology (2001), 21(10), 655-663

Among shoots of Fraxinus angustifolia Vahl raised in vitro, 76% rooted after culture on root induction medium for 5 days in darkness followed by culture on root expression medium for 15 days in light. The ... [more ▼]

Among shoots of Fraxinus angustifolia Vahl raised in vitro, 76% rooted after culture on root induction medium for 5 days in darkness followed by culture on root expression medium for 15 days in light. The addition of 20.7 muM indole-butyric acid (IBA) to the root induction medium did not significantly increase the rooting percentage (88%). Putrescine, spermidine, cyclohexylamine (CHA) and aminoguanidine (AG) enhanced rooting up to 100% (98.66% for AG), when applied during root induction in the absence of IBA, otherwise these compounds inhibited rooting, as did spermine and difluoromethylornithine (DFMO) + difluoromethylarginine (DFMA). The root induction phase was characterized by a temporary increase in endogenous free indole-acetic acid (IAA) and putrescine concentrations during root induction, whereas the root expression phase was characterized by increased peroxidase activity and low concentrations of polyamines. These changes were specifically associated with the rooting process and did not depend on the presence of exogenous IBA, because application of exogenous IBA enhanced the amount of IAA in the cuttings but did not affect rooting or the pattern of changes in polyamines and peroxidase. The effects of CHA, AG and DFMO + DFMA on endogenous concentrations of auxins and polyamines highlight the close relationship between the effects of IAA and putrescine in root induction and suggest that polyamine catabolism has an important role in root formation and elongation. [less ▲]

Detailed reference viewed: 37 (7 ULg)
Full Text
Peer Reviewed
See detailHuman - ovine comparative sequencing of a 250 kilobase imprinted domain encompassing the callipyge (clpg) gene and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11 and MEG8
Charlier, Carole ULg; SEGERS, Karin ULg; Wagenaar, D. et al

in Genome Research (2001), 11

Two ovine BAC clones and a connecting long-range PCR product, jointly spanning ∼250 kb and representing most of theMULGE5-OY3 marker interval known to contain the clpglocus, were completely sequenced. The ... [more ▼]

Two ovine BAC clones and a connecting long-range PCR product, jointly spanning ∼250 kb and representing most of theMULGE5-OY3 marker interval known to contain the clpglocus, were completely sequenced. The resulting genomic sequence was aligned with its human ortholog and extensively annotated. Six transcripts, four of which were novel, were predicted to originate from within the analyzed region and their existence confirmed experimentally: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. RT-PCR experiments performed on a range of tissues sampled from an 8-wk-old animal demonstrated the preferential expression of all six transcripts in skeletal muscle, which suggests that they are under control of common regulatory elements. The six transcripts were also shown to be subject to parental imprinting: DLK1, DAT, andPEG11 were shown to be paternally expressed and GTL2,antiPEG11, and MEG8 to be maternally expressed. [less ▲]

Detailed reference viewed: 30 (4 ULg)
Full Text
Peer Reviewed
See detailEvolution of aphidophagous ladybird populations in a vegetable crop and implications as biological agents.
Francis, Frédéric ULg; Colignon, P.; Hastir, P. et al

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (2001), 66(2a), 333-40

Aphidophagous predators such as hoverfly and ladybird beetles are effective biological agents to control aphid pests in perennial and annual cultivated species. Introduction and conservation of beneficial ... [more ▼]

Aphidophagous predators such as hoverfly and ladybird beetles are effective biological agents to control aphid pests in perennial and annual cultivated species. Introduction and conservation of beneficial insects are two ways to increase natural control of pests. Whether massive releases of entomophagous insects are expensive and time consuming, the preservation of predator natural populations can be expected by reducing and by adapting chemical treatments in crop fields. Vegetable cultivated areas increased in Belgium for several years, mainly Fabaceae species such as peas and beans. In this work, the evolution of ladybird species population was assessed from May to June in broad bean fields (Vicia faba L.) between Waremme and Hannut, in Hesbaye. Weekly, the aphid and aphidophagous beetle populations were collected from yellow traps and determined on plants by visual observations. Even if five ladybird species were identified, three of them represented more than 95% of the collected insects (Coccinella septempunctata L., Propylea quatuor-decimpunctata L. and Psyllobora vingintiduopunctata L.). Evolution of coccinellid populations during the cultivation season was discussed in relation to the presence of potential aphid preys and the agrochemical treatments which were applied. Integrated pest management in vegetable fields constitute a reliable way to increase the quality level of fresh vegetables in terms of pesticide residue limitations. [less ▲]

Detailed reference viewed: 57 (5 ULg)
Full Text
Peer Reviewed
See detailInsémination artificielle des vaches ‘Azawak’ et taurin ‘Gourunsi’ au Burkina Faso
Zongo, M.; Boly, H.; Sawadogo, L. et al

in Tropicultura (2001), 19(2), 75-78

La synchronisation des chaleurs et la fertilité en insémination artificielle des zébus ‘Azawak’ (Bos indicus, n = 66) et des taurins ‘Gourunsi’ (Bos taurus, n = 20) ont été appréciées en zone périurbaine ... [more ▼]

La synchronisation des chaleurs et la fertilité en insémination artificielle des zébus ‘Azawak’ (Bos indicus, n = 66) et des taurins ‘Gourunsi’ (Bos taurus, n = 20) ont été appréciées en zone périurbaine soudano-sahélienne de Ouagadougou au Burkina Faso. La synchronisation a été effectuée avec des implants sous cutanés de progestagène placés sous la peau de l’oreille pendant 10 jours. Le traitement a été complété par l’injection de prostaglandine F2 et de PMSG, respectivement au 8ième et au 10ième jours de la pose de l’implant. Le taux d’œstrus induits observés par l’éleveur était de 94,2% dans les 48 heures qui suivaient le retrait de l'implant. Il variait significativement (P<0,05) selon les groupes zébus ‘Azawak’ et taurins ‘Gourunsi’ (100% et 75%, respectivement). Les taux de fertilité, déterminés par palpation rectale chez les zébus ‘Azawak’ et les taurins ‘Gourunsi’, ont été respectivement 24,2% et 10%. La fertilité a varié selon le moment de l’insémination: lorsque les femelles étaient inséminées 36 à 48 heures après le retrait de l’implant, les taux de fertilité ont été faibles (0 à 12,5% chez les taurins ‘Gourunsi’ et les zébus ‘Azawak’, respectivement). Par ailleurs, lorsque les inséminations étaient réalisées 24 à 36 heures après le retrait de l’implant, les taux de fertilité ont été respectivement de 50% et 42,3%. [less ▲]

Detailed reference viewed: 118 (5 ULg)
See detailLe déroulement de la conférence sur le développement rural durable dans l'ensemble des pays d'Europe.
Christians, Charles ULg; Schmitz, Serge ULg

in Bulletin de la Société Géographique de Liège (2001), 41

Présentation de l'organisation, du thème, de l'objectif et des activités (travaux sur le terrain et Conférence).

Detailed reference viewed: 24 (2 ULg)
Full Text
See detailÉtude de l'influence de l'hydrodynamique sur une bactérie filamenteuse par analyse d'images
Groslambert, Sylvie ULg; Blacher, Silvia ULg; Crine, Michel ULg

in Récents Progrès en Génie des Procédés (2001), 78(18), 153-158

Detailed reference viewed: 9 (3 ULg)
Full Text
Peer Reviewed
See detailAb initio simulations of liquid semiconductors using the pseudopotential-density functional method
Chelikowsky, J. R.; Derby, J. J.; Godlevsky, V. V. et al

in Journal of Physics : Condensed Matter (2001), 13(41), 817-854854

One of the most difficult problems in condensed matter physics is describing the microscopic nature of the liquid state. Owing to the dynamical nature of the liquid state, it is not possible to discuss a ... [more ▼]

One of the most difficult problems in condensed matter physics is describing the microscopic nature of the liquid state. Owing to the dynamical nature of the liquid state, it is not possible to discuss a particular microscopic structure; only ensemble averages can be specified. Such averages can be performed via well crafted molecular dynamics simulations: the length of the simulation, the size of the ensemble and the nature of the interatomic forces must all be carefully analysed. Historically, a problematic issue in doing such simulations is that of how to describe the interatomic forces in the liquid state. This matter is especially challenging for the melt of semiconductors, such as silicon or gallium arsenide, where the chemical bond contains a strong covalent component. It is difficult to use pairwise interatomic potentials in such cases. Although many-body potentials can be utilized for simulations of these materials, one must map quantum phenomena such as hybridization onto classical interatomic potentials. This mapping is complex and difficult. In this review, we illustrate how one can avoid this problem by utilizing quantum forces to simulate liquids. Our focus is on the pseudopotential-density functional method. Within the pseudopotential method, only the valence electrons are explicitly treated and within the density functional theory, exchange and correlation terms are mapped onto an effective one-electron potential [less ▲]

Detailed reference viewed: 26 (1 ULg)
Full Text
See detailQuelle biodiversite pour nos lisieres forestieres?
Branquart, E.; Doucet, Jean-Louis ULg; Liesse, D. et al

in Parcs & Réserves (2001), 56(1), 26-32

Detailed reference viewed: 63 (8 ULg)
Full Text
See detailAperçu du régime alimentaire du Grand Cormoran (Phalacrocorax carbo) dans les eaux intérieures du Pas-de-Calais (France)
Libois, Roland ULg

in Aves (2001), 38(2), 49-59

The stomach contents of 40 great cormorants shot during "regulation" operations in the Pas-de-Calais have been analysed. Eight stomachs were empty and 426 fishes belonging to 15 species were recovered in ... [more ▼]

The stomach contents of 40 great cormorants shot during "regulation" operations in the Pas-de-Calais have been analysed. Eight stomachs were empty and 426 fishes belonging to 15 species were recovered in the others. The dominant prey is the roach. Other regular prey are the common bream and the perch. Other fish (Salmonids, pike, pikeperch, ruffe, rudd, lamprey) appear in less than 5 stomachs. Most of the prey are small sized fish, less than 12 cm (total length). The biomass of 80% of the preys is less than 20g. However, the role of the greater fish in the diet must be emphasized: those weighing more than 200g (1% in numbers) reprensent 20% of the ingested biomass. [less ▲]

Detailed reference viewed: 86 (3 ULg)
Full Text
Peer Reviewed
See detailSymptoms modifying effect of avocado/soybean unsaponifiables (ASU) in knee osteoarthritis. A double blind, prospective, placebo-controlled study.
Appelboom, T; Schuermans, J; Verbruggen, G et al

in Scandinavian Journal of Rheumatology (2001), 30(4), 242-7

OBJECTIVES: We compare the symptomatic effects of 300 or 600mg daily of ASU in patients with knee osteoarthritis. METHODS: A multicenter, double blind, study comparing a daily intake of 300mg or 600mg of ... [more ▼]

OBJECTIVES: We compare the symptomatic effects of 300 or 600mg daily of ASU in patients with knee osteoarthritis. METHODS: A multicenter, double blind, study comparing a daily intake of 300mg or 600mg of ASU and placebo. The study lasted 3 months and involved patients of both genders, aged 45 to 80 years and presenting with femoro-tibial knee osteoarthritis. The primary endpoint was NSAIDs and analgesics intake between D30 and D90. RESULTS: All efficacy parameters were significantly improved (p<0.01), in the two ASU groups compared to the placebo group. At D90, NSAIDs and analgesics intake decreased by more than 50% in 71% of the patients receiving ASU 300mg or 600mg, compared to 36% of the patients receiving placebo. From DO to D90 Lequesne's index dropped by 3.9 and 2.9 points in ASU 300mg and 600mg groups, respectively, against 1.6 in those receiving placebo. CONCLUSION: The efficacy of ASU at a dosage of 300mg/day and 600mg/day was consistently superior to that of placebo at all endpoints, with no differences observed between the two doses. [less ▲]

Detailed reference viewed: 38 (5 ULg)
Full Text
Peer Reviewed
See detailComment je traite une ascite
Gielen, S.; Delwaide, Jean ULg; Detry, Olivier ULg et al

in Revue Médicale de Liège (2001), 56(12), 809-815

Ascites is the most common of the major complications of cirrhosis. The initial evaluation of a patient with ascites should include a history, physical evaluation and some investigations. Treatment should ... [more ▼]

Ascites is the most common of the major complications of cirrhosis. The initial evaluation of a patient with ascites should include a history, physical evaluation and some investigations. Treatment should consist of treating the underlying liver disease, sodium restricted diet (2 g of Na+/day) and diuretics. This regimen is effective in 90 % of patients. The treatment options for the diuretic-resistant patients include serial therapeutic paracentesis, peritoneovenous shunting, TIPSand liver transplantation. The treatment and prophylaxis of spontaneous bacterial peritonitis which is a frequent and severe complication in cirrhotic patients with ascites is also important. The differential diagnosis with secondary bacterial peritonitisis is essential because the latter usually does not resolve unless patients are surgically treated. [less ▲]

Detailed reference viewed: 88 (4 ULg)
Peer Reviewed
See detailNicardipine Protocol for Cabg Using the Radial Artery Clinical and Angiographic Data
RADERMECKER, Marc ULg; Grenade, Thierry ULg; Cao-Thian, S. K. et al

in Acta Chirurgica Belgica (2001), 101(4, Jul-Aug), 185-9

The routine use of arterial grafts in coronary surgery is facilitated by peroperative adjunction of antispasmodic drug to reduce the event of spasm. Diltiazem has been favoured in most clinical studies ... [more ▼]

The routine use of arterial grafts in coronary surgery is facilitated by peroperative adjunction of antispasmodic drug to reduce the event of spasm. Diltiazem has been favoured in most clinical studies devoted to the radial artery graft. The aim of this study was to assess the efficacy of a spasm preventing protocol associating hydrostatic dilation of the graft with a diluted solution of papaverine and nicardipine infusion, starting preoperatively and continued postoperatively in i.v. and per os forms. Between September 1996 and March 1997, a consecutive series of 50 patients underwent myocardial revascularization using the radial artery. The radial artery was prepared by hydrostatic dilation with papaverine (1%) and nicardipine was administrated at 0.25 microgram/kg/min and titrated according to the arterial systemic pressure. Operative mortality was 4% (sepsis). There was no evidence of perioperative MI nor hypoperfusion syndrome. Mean CKMB level at 18 hours was 36 micrograms/l. No ischaemic anomalies of the ECG were detected. Angiography performed in the last 20 patients showed a 98% (51/52) permeability rate for all graft; 19/20 radial grafts (95%) were patent. One radial graft presented a 50% stenosis at the proximal anastomosis, and another a moderate spasm (40%) in the middle part of the conduit. This study confirms that the radial artery conduit can be used with satisfactory results for routine coronary artery bypass. The use of nicardipine allows the control the vasoreactivity of the radial graft without totally obviating at least angiographic spasm. This drug is easy to titrate, and well tolerated in association to beta-blockers in the routine perioperative management of the coronary patients. [less ▲]

Detailed reference viewed: 51 (10 ULg)
Peer Reviewed
See detailPotential of the Migraine Disability Assessment (MIDAS) Questionnaire as a public health initiative and in clinical practice.
Edmeads, J.; Lainez, J. M.; Brandes, J. L. et al

in Neurology (2001), 56(6 Suppl 1), 29-34

Migraine is not always well managed in clinical practice, often being under-diagnosed and under-treated. As a result, many sufferers never consult a physician or lapse from care after physician contact ... [more ▼]

Migraine is not always well managed in clinical practice, often being under-diagnosed and under-treated. As a result, many sufferers never consult a physician or lapse from care after physician contact. Although most migraine care is provided by general practitioners, others, including specialists, emergency room physicians, pharmacists, and alternative practitioners, may also be involved. A method of standardizing clinical information about migraine is essential for coordinated, logical, and systematic care. The impact of migraine on the patient is an important clinical parameter but one that is seldom inquired about, perhaps because it exhibits such marked variability among and within individuals. Headache-related disability can be an objective and measurable index of this impact. The Migraine Disability Assessment (MIDAS) Questionnaire is a simple and validated instrument with potential for use in clinical practice, research, and public health. It can improve communication between patients and health-care professionals regarding the impact of migraine which, in turn, allows tailoring of the intensity of treatment to the severity of the illness. Changes in the MIDAS score may serve as an end point in assessing treatment efficacy. In populations, MIDAS scores may indicate the burden of migraine in the community and spark public health initiatives to improve management. [less ▲]

Detailed reference viewed: 42 (2 ULg)
Full Text
Peer Reviewed
See detailA finite element formulation for spectral problems in optical fibers
Guenneau, S.; Nicolet, A.; Zolla, F. et al

in COMPEL (2001), 20(1), 120--131

Detailed reference viewed: 9 (2 ULg)
Peer Reviewed
See detailRevenant & exorcisme à Liège. Quête de reliques en Sardaigne (1634-1652)
George, Philippe ULg

in Bulletin de la Commission Royale d'Histoire (2001), CLXVII

Detailed reference viewed: 62 (11 ULg)
Peer Reviewed
See detailIatrogenic thyrotoxicosis. Causal circumstances, pathophysiology and principles of treatment. Review of the literature
Meurisse, Michel ULg; Preud'Homme, Laurence ULg; Lamberty, Geoffrey ULg et al

in Acta Chirurgica Belgica (2001), 101(6), 257-266

Thyrotoxicosis is the clinical syndrome that results when tissues are exposed to high levels of circulating thyroid hormones. In most instances, thyrotoxicosis is due to hyperthyroidism, a term reserved ... [more ▼]

Thyrotoxicosis is the clinical syndrome that results when tissues are exposed to high levels of circulating thyroid hormones. In most instances, thyrotoxicosis is due to hyperthyroidism, a term reserved for disorders characterized by overproduction of thyroid hormones by the thyroid gland. Nevertheless, thyrotoxicosis may also result from a variety of conditions other than thyroid hyperfunction. The present report focuses on the etiologies, pathophysiology and treatment of iatrogenic thyrotoxicosis. Iatrogenic thyrotoxicosis may be caused by 1) subacute thyroiditis (a result of lymphocytic infiltration, cellular injury, trauma or radiation) with release of preformed hormones into circulation, 2) excessive ingestion of thyroid hormones ("thyrotoxicosis factitia"), 3) iodine-induced hyperthyroidism (radiological contrast agents, topical antiseptics or other medications). Among these causes of iatrogenic thyrotoxicosis, that induced by the iodine overload and cytotoxicity associated with amiodarone represents a significant challenge. Successful management of amiodarone-induced thyrotoxicosis requires close cooperation between endocrinologists and endocrine Surgeons. Surgical treatment may have a leading yet often underestimated role in view of the potential life-threatening severity of this disease, whereas others kinds of iatrogenic thyrotoxicosis are usually treated conservatively. [less ▲]

Detailed reference viewed: 26 (1 ULg)
See detailDernier hoquet à Francorchamps
Abu Dalu, Firass ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2001)

Detailed reference viewed: 27 (0 ULg)
Peer Reviewed
See detailPalaeogeographic and palaeoenvironmental characteristics of major marine incursions in northwestern Europe during the Westphalian C (Bolsovian).
Dusar, M; Paproth, E; Streel, Maurice ULg et al

in Geologica Belgica (2001), (2000)3(3-4), 331-347

Detailed reference viewed: 36 (2 ULg)
Full Text
Peer Reviewed
See detailProcollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis.
Fernandes, R. J.; Hirohata, S.; Engle, J. M. et al

in Journal of Biological Chemistry (2001), 276(34), 31502-9

The amino and carboxyl propeptides of procollagens I and II are removed by specific enzymes as a prerequisite for fibril assembly. Null mutations in procollagen I N-propeptidase (ADAMTS-2) cause ... [more ▼]

The amino and carboxyl propeptides of procollagens I and II are removed by specific enzymes as a prerequisite for fibril assembly. Null mutations in procollagen I N-propeptidase (ADAMTS-2) cause dermatosparaxis in cattle and the Ehlers-Danlos syndrome (dermatosparactic type) in humans by preventing proteolytic excision of the N-propeptide of procollagen I. We have found that procollagen II is processed normally in dermatosparactic nasal cartilage, suggesting the existence of another N-propeptidase(s). We investigated such a role for ADAMTS-3 in Swarm rat chondrosarcoma RCS-LTC cells, which fail to process the procollagen II N-propeptide. Stable transfection of RCS-LTC cells with bovine ADAMTS-2 or human ADAMTS-3 partially rescued the processing defect, suggesting that ADAMTS-3 has procollagen II N-propeptidase activity. Human skin and skin fibroblasts showed 30-fold higher mRNA levels of ADAMTS-2 than ADAMTS-3, whereas ADAMTS-3 mRNA was 5-fold higher than ADAMTS-2 mRNA in human cartilage. We propose that both ADAMTS-2 and ADAMTS-3 process procollagen II, but ADAMTS-3 is physiologically more relevant, given its preferred expression in cartilage. The findings provide an explanation for the sparing of cartilage in dermatosparaxis and, perhaps, for the relative sparing of some procollagen I-containing tissues. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailA comparison of blade tip timing data analysis methods
Carrington, Ian B; Wright, Jan Robert; Cooper, Jonathan E et al

in Proceedings of the Institution of Mechanical Engineers - Part G - Journal of Aerospace Engineering (2001), 215(6), 301-312

The experimental determination of the vibration characteristics of rotating engine blades is very important for fatigue failure considerations. One of the most promising techniques for measuring the ... [more ▼]

The experimental determination of the vibration characteristics of rotating engine blades is very important for fatigue failure considerations. One of the most promising techniques for measuring the frequency of blade vibrations is blade tip timing. In this paper, three vibration analysis methods were specifically formulated and applied to the tip timing problem for the first time, using data obtained from a simple mathematical blade tip timing simulation. The results from the methods were compared statistically in order to determine which of the techniques is more suitable. One of the methods, the global autoregressive instrumental variables approach, produced satisfactory results at realistic noise levels. However, all of the techniques produced biased results under certain circumstances. [less ▲]

Detailed reference viewed: 138 (2 ULg)
Peer Reviewed
See detailL'etude clinique du mois. L'etude CALM ou interet de combiner un inhibiteur de l'enzyme de conversion et un antagoniste du recepteur de type 1 de l'angiotensine II dans le traitement de la nephropathie diabetique.
Philips, Jean-Christophe ULg; Weekers, Laurent ULg; Scheen, André ULg

in Revue Médicale de Liège (2001), 56(2), 126-8

The main objective of the CALM (Candesartan And Lisinopril Microalbuminuria) study is to assess the effect of a dual blockade of the renin-angiotensin system--using both an angiotensin converting enzyme ... [more ▼]

The main objective of the CALM (Candesartan And Lisinopril Microalbuminuria) study is to assess the effect of a dual blockade of the renin-angiotensin system--using both an angiotensin converting enzyme inhibitor (ACE-I) and an angiotensin II type 1 receptor blocker--in patients with type 2 diabetes, high blood pressure and microalbuminuria. The study included 200 patients randomized to receive candesartan 16 mg or lisinopril 20 mg for 12 weeks, followed by 12 weeks of the same monotherapy or a combination treatment. Main outcomes are the reduction of microalbuminuria and blood pressure. All three of the treatments are effective, but the dual blockade is respectively 18%, 8 mmHg and 5 mmHg more effective in reducing microalbuminuria, systolic and diastolic blood pressure. No comparison is made between this "new" association and the more frequently used biotherapy (i.e. ACE-I plus thiazidic diuretic) and therefore its usefulness in regular practice is still to be determined. [less ▲]

Detailed reference viewed: 155 (0 ULg)
Full Text
See detailProthèses péniennes : taille du pénis implanté et dimensions de la virilité
Kempeneers, Philippe ULg; Andrianne, Robert ULg; Mormont, Christian ULg et al

in Cahiers de Sexologie Clinique (2001), 27(149), 18-27

The implantation of a penile prosthesis frequently results in a reduction of the erectile length compared with natural erections. Some patients complain about this fact and their satisfaction at ... [more ▼]

The implantation of a penile prosthesis frequently results in a reduction of the erectile length compared with natural erections. Some patients complain about this fact and their satisfaction at prosthesis assisted sexuality is significantly diminished. Obviously, the degree of complaint is not proportional to the objective degree of penile reduction. For an objective reality to lead to complaints, subjective criteria of appreciation have to intervene. A survey questionnaire completed by 39 implanted patients and 30 partners shows that complaints about the size of the implanted penis are mostly related to a social representation of sexuality which associates the male erotic value to criteria of erectile length, spontaneity and “naturalness”. This representation also tends to be projected in the partner’s mind, independently of her actual attitudes and feelings. Therefore, the injurious impact of penile reduction on satisfaction at the prosthetic device seems to be inseparable from a global representation of prosthetic sexuality which is experienced as a “parodic”, “reduced” virility. The reduced size of the penis focuses feelings of vexation which cannot be reduced to simple functional considerations, it embodies the painful and complex image of a lost virility. From this point of view, psychosexological counselling would be indicated in order to help some couples to improve their adaptation to penile prosthesis. Moreover, an Attitude scale towards Penile Implant (API) derived from the survey questionnaire might enable the identification of risk-patients. [less ▲]

Detailed reference viewed: 901 (14 ULg)
Full Text
Peer Reviewed
See detailThe effects of JL 13, a pyridobenzoxazepine with potential atypical antipsychotic activity, in animal models for schizophrenia
Ellenbroek, Bart; Liégeois, Jean-François ULg; Bruhwyler, Jacques et al

in Journal of Pharmacology and Experimental Therapeutics (2001), 298

Detailed reference viewed: 9 (0 ULg)
Full Text
See detailBelgique: l'euthanasie pourrait ne pas constituer une infraction
Mormont, Christian ULg

in Courrier de l'éthique médicale (2001), 1(2), 33-34

Detailed reference viewed: 47 (1 ULg)
Full Text
Peer Reviewed
See detailDistinct carbohydrate recognition domains of an invertebrate defense molecule recognize Gram-negative and Gram-positive bacteria.
Bilej, M.; De Baetselier, P.; Van Dijck, E. et al

in Journal of Biological Chemistry (2001), 276(49), 45840-7

Coelomic fluid of Eisenia foetida earthworms (Oligochaeta, Annelida) contains a 42-kDa defense molecule named CCF for coelomic cytolytic factor. By binding microbial antigens, namely the O-antigen of ... [more ▼]

Coelomic fluid of Eisenia foetida earthworms (Oligochaeta, Annelida) contains a 42-kDa defense molecule named CCF for coelomic cytolytic factor. By binding microbial antigens, namely the O-antigen of lipopolysaccharide (LPS), beta-1,3-glucans, or N,N'-diacetylchitobiose present, respectively, on Gram-negative bacteria or yeast cell walls, CCF triggers the prophenoloxidase activating pathway. We report that CCF recognizes lysozyme-predigested Gram-positive bacteria or the peptidoglycan constituent muramyl dipeptide as well as muramic acid. To identify the pattern recognition domains of CCF, deletion mutants were tested for their ability to reconstitute the prophenoloxidase cascade in E. foetida coelomic fluid depleted of endogenous CCF in the presence of LPS, beta-1,3-glucans, N,N'-diacetylchitobiose, and muramic acid. In addition, affinity chromatography of CCF peptides was performed on immobilized beta-1,3-glucans or N,N'-diacetylchitobiose. We found that the broad specificity of CCF for pathogen-associated molecular patterns results from the presence of two distinct pattern recognition domains. One domain, which shows homology with the polysaccharide and glucanase motifs of beta-1,3-glucanases and invertebrate defense molecules located in the central part of the CCF polypeptide chain, interacts with LPS and beta-1,3-glucans. The C-terminal tryptophan-rich domain mediates interactions of CCF with N,N'-diacetylchitobiose and muramic acid. These data provide evidence for the presence of spatially distinct carbohydrate recognition domains within this invertebrate defense molecule. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailImage du mois. Abces cerebral et toxoplasmose.
Omazic, A. F.; Welter, P.; Piette, Jacques ULg et al

in Revue Médicale de Liège (2001), 56(8), 541-2

Detailed reference viewed: 24 (6 ULg)
Full Text
Peer Reviewed
See detailUretere retrocave chez un enfant de dix ans.
Kakanou, A.; NCHIMI LONGANG, Alain ULg; Ghuysen, M. S. et al

in Annales de Chirurgie (2001), 126(2), 156-8

Retrocaval ureter is an uncommon abnormality of the inferior vena cava, which is rarely detected in the child due to its non-specific symptomatology. Despite a varying state of severity, chronic ureteral ... [more ▼]

Retrocaval ureter is an uncommon abnormality of the inferior vena cava, which is rarely detected in the child due to its non-specific symptomatology. Despite a varying state of severity, chronic ureteral obstruction generally leads to the deterioration of renal function during adulthood. Treatment depends on the symptomatology in question, and surgical management should be as conservative as possible. [less ▲]

Detailed reference viewed: 11 (2 ULg)
See detailPropositions relatives à l'enseignement
de Landsheere, Gilbert ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2001), (5-6), 79-84

Comme pour le texte 'L'avenir de l'enseignement' qui est publié dans ces Cahiers, il s'agit d'un texte qui date d'une dizaine d'années. Ces propositions ont été formulées peu de temps après la ... [more ▼]

Comme pour le texte 'L'avenir de l'enseignement' qui est publié dans ces Cahiers, il s'agit d'un texte qui date d'une dizaine d'années. Ces propositions ont été formulées peu de temps après la communautarisation complète de l'enseignement. A présent que 'nous sommes maîtres chez nous', qu'en est-il ? Quel chemin avons-nous parcouru ? S'agissait-il de prémonitions ? De rêves et d'utopies ? Chacun peut s'en faire une idée. Chacun peut aussi apprécier, sur la base d'un texte, ce qui se disait alors et ce que Gilbert de Landsheere envisageait comme souhaitable. [less ▲]

Detailed reference viewed: 46 (8 ULg)
Full Text
Peer Reviewed
See detailLarge pion pole in Z(S)(MOM)/(Z(P)(MOM) from Wilson action data
Cudell, Jean-René ULg; Le Yaouanc, A.; Pittori, C.

in Physics Letters B (2001), 516(1-2), 92-102

Detailed reference viewed: 16 (4 ULg)
Full Text
Peer Reviewed
See detailGenesis of clone size heterogeneity in megakaryocytic and other hemopoietic colonies: the stochastic model revisited
Paulus, Jean-Michel ULg; Levin, Jack; Debili, Najet et al

in International Society for Experimental Hematology (2001), 29(nd), 1256-69

OBJECTIVE: We previously showed that the distributions of the numbers of doublings (NbD) undergone by individual megakaryocyte progenitors before commitment to polyploidization are markedly skewed and can ... [more ▼]

OBJECTIVE: We previously showed that the distributions of the numbers of doublings (NbD) undergone by individual megakaryocyte progenitors before commitment to polyploidization are markedly skewed and can consistently be fitted to straight lines when plotted on semilogarithmic coordinates. The slope of such lines, which yields the probability of polyploidization per doubling, is made less steep by stimulators of megakaryocyte colony formation and is less steep in mixed erythroid-megakaryocyte than in pure megakaryocyte colonies. Therefore, megakaryocytopoiesis provides a unique model for the study of clonal heterogeneity in a hemopoietic lineage, which is the subject of this review. DATA SOURCES: Articles relevant to the interpretation of these data were selected from the authors' and public databases. DATA SYNTHESIS: Exponential NbD distributions were first explained by postulating that following the assembly of thrombopoiesis-specific regulators, megakaryocyte progenitors require only a single random event to arrest proliferation and commit to polyploidization. However, this stochastic model was refuted by data indicating that intrinsic properties of individual progenitors affect the NbD they achieve. We suggest that the unequal repartition of critical compounds (including receptors, signaling molecules, and gene regulators) inherent in the stem cell-progenitor transition causes a heritable heterogeneity in megakaryocyte progenitor responsiveness to polyploidization inducers. This model would be compatible with 1) the evidence for intraclonal synchronization in megakaryocyte and other hemopoietic clones generated by committed progenitors; 2) the low probability of polyploidization of the relatively insensitive bipotent megakaryocyte progenitors; and 3) the thesis that stimulators act in part by recruiting megakaryocyte progenitor cells endowed with lesser responsiveness to polyploidization inducers and higher proliferative potential. CONCLUSION: The responsiveness of individual megakaryocyte progenitors to polyploidization inducers may be a major determinant of the exponential shape of NbD distributions. [less ▲]

Detailed reference viewed: 29 (6 ULg)
Full Text
Peer Reviewed
See detailLiquid distribution images on structured packing by X-ray computed tomography
Marchot, Pierre ULg; Toye, Dominique ULg; Pelsser, A. M. et al

in AIChE Journal (2001), 47(6), 1471-1476

Detailed reference viewed: 33 (5 ULg)
Full Text
Peer Reviewed
See detailNovel plasma extraction procedure and development of a specific enzyme immunoassay of oxytocin: application to biological and clinical investigations of small-cell carcinoma of the lung
Pequeux, Christel ULg; Hendrick, Jean-Claude; Hagelstein, Marie-Thérèse ULg et al

in Scandinavian Journal of Clinical & Laboratory Investigation (2001), 61(5), 407-415

Paraneoplastic secretion of the lactation-inducing hormone oxytocin (OT) has been reported in about 30% of cases of small cell carcinoma of the lung (SCCL). In order to investigate the role of OT in the ... [more ▼]

Paraneoplastic secretion of the lactation-inducing hormone oxytocin (OT) has been reported in about 30% of cases of small cell carcinoma of the lung (SCCL). In order to investigate the role of OT in the biology of SCCL tumours, a specific enzyme-immunoassay(EIA) for OT, which can be applied to both human plasma <br />and culturemedium, has been developed. OT EIA is performed on 96-well microtiter plates coated with a rabbit polyclonal antibody (Ab) anti-OT (O4). This antibody does not exhibit any significant cross-reactivity either with vasopressin (VP) or with vasotocin (VT). The immunological reaction involving Ab anti-OT is a competition between the tracer (biotinylatedOT) and syntheticOT (standard curve) or OT present in biological samples. In order to limit interference induced by plasma proteins, plasma samples are titrated by a one-step centrifugation on centricon YM-3 (cut-oOE 3000 Da). After plasma filtration, 90.7 ± 5.1 (SD) % (n = 22) immunoreactive ( IR) OT is recovered. The sensitivity of OT EIA is 1 pmol/L, while intra- and inter-assay coefficients of variation (CV) are around 3.41% and 2.84%, respectively. In healthy volunteers, plasma IR OT is 7.28 ± 4.49 (SD) pmol/L (n = 32) with no gender diOEerence. As shown by the data both from plasma of SCCL patients and from supernatants and cell contents of SCCL cell lines, this EIA procedure offers a novel, reproducible, specific and sensitive method for the measurement of IR OT. [less ▲]

Detailed reference viewed: 84 (13 ULg)
Full Text
Peer Reviewed
See detailA Parametric Distance Function Approach for Malmquist Productivity Index Estimation
Fuentes, Hugo J.; Grifell-Tatjé, Emili; Perelman, Sergio ULg

in Journal of Productivity Analysis (2001), 15

Malmquist indexes of productivity are generally estimated using index number techniques or non-parametric frontier approaches. The aim of this paper is to showthat Malmquist indexes can be estimated in a ... [more ▼]

Malmquist indexes of productivity are generally estimated using index number techniques or non-parametric frontier approaches. The aim of this paper is to showthat Malmquist indexes can be estimated in a similar way using parametric-deterministic or parametric-stochastic frontier approaches. To allow a multi-output multi-input technology and for technical change in production, we adopt an output distance function which is specified in a translog form. We then show that using the estimated parameters, several radial distance functions can be calculated and combined in order to estimate and decompose the productivity index. Finally, this approach is applied to a panel of Spanish insurance companies. The main results confirm those generally obtained for financial services: very low rates of growth and technical change in spite of a rapid deregulation process and expansion of activity. [less ▲]

Detailed reference viewed: 108 (2 ULg)
Peer Reviewed
See detailSmall Town China. Goverance, Economy, Environment and Lifestyle in Three Zhen
Florence, Eric ULg

in Revue Bibliographique de Sinologie = Review of Bibliography in Sinology (2001), Vol. XVIII

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailTraçabilité dans la filière viande. I. La traçabilité administrative.
Evrard, V.; China, B.; Noirfalise, R. et al

in Annales de Médecine Vétérinaire (2001), 145

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une ... [more ▼]

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une perturbation du marché de la viande. Pour redresser l’image des produits carnés belges, il est important de pouvoir en déterminer et en garantir l’origine. En Belgique, il existe divers systèmes de traçabilité administrative dont le principal est le système SANITEL qui comprend un système automatisé de traitement de données relatives à l’identification et l’enregistrement des animaux. Au-delà de l’aspect légal et réglementaire, différentes initiatives, visant une amélioration de la qualité, fleurissent : "les labels". Ceux-ci intègrent fréquemment la traçabilité dans leur cahier des charges. La traçabilité administrative n’est pas infaillible, la perte de documents et les fraudes peuvent ternir l’image de celle-ci. C’est pourquoi le système documentaire a été associé aux empreintes génétiques des animaux. 5 [less ▲]

Detailed reference viewed: 122 (3 ULg)
Full Text
Peer Reviewed
See detailSémiologie cardiaque dans l’espèce équine. 1- L’anamnèse et l’examen clinique.
Amory, Hélène ULg

in Pratique Vétérinaire Equine (2001), 33

Detailed reference viewed: 47 (3 ULg)
See detailEugène Monseur (1860-1912)
Boutier, Marie-Guy ULg

in Wallonnes : Chronique de la Société de Langue et de Littérature Wallonnes (2001), (1),

Detailed reference viewed: 39 (1 ULg)
Full Text
Peer Reviewed
See detailThe early Paleozoic carbon cycle
Godderis, Y.; François, Louis ULg; Veizer, J.

in Earth And Planetary Science Letters (2001), 190(3-4), 181-196

A review of O, C, Sr and S isotope trends for the entire Phanerozoic shows that the present-day values of isotope signals are similar to those at the Proterozoic termination. The sharp rise in Sr-87/Sr-86 ... [more ▼]

A review of O, C, Sr and S isotope trends for the entire Phanerozoic shows that the present-day values of isotope signals are similar to those at the Proterozoic termination. The sharp rise in Sr-87/Sr-86 since similar to 65 Ma has been attributed to an uplift and subsequent metamorphism and erosion associated with the Himalayas and Tibet. This orogenic evolution has been postulated to have influenced the global organic and inorganic carbon cycles and climate as well. A similar large-scale orogeny, the Pan-African event, also dominated the Neoproterozoic (Vendian) times, and the similarity of modern and Neoproterozoic isotope values for seawater may therefore have had a comparable tectonic cause. In this contribution, we present the results of a numerical model of the coupled C-alkalinity-S-Sr cycles suggesting that the early Paleozoic (from early Cambrian to late Devonian) evolution of Sr, O, C and S seawater isotope signals could have been the consequence of progressive oxidation of a large reduced carbon reservoir exhumed during the Pan-African orogeny. The delta O-18 measured in brachiopod shells is used as a forcing of the model, postulating that any change in the oxygen isotopic composition of seawater is the result of a disequilibrium in the organic carbon subcycle through the coupling of the oxygen isotopic and carbon cycles. The calculated delta C-13, Sr-87/Sr-86 and delta S-34 are in good agreement with the data, as is the reasonable calculated history for atmospheric pCO(2) and its relation to global climate. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailSexual compatibility between two heterochronic morphs in the Alpine newt, Triturus alpestris
Denoël, Mathieu ULg; Poncin, Pascal ULg; Ruwet, Jean-Claude

in Animal Behaviour (2001), 62(3), 559-566

Paedomorphosis, in which individuals retain ancestral characteristics in the adult stage, is widespread in newts and salamanders and is suspected to play an important role in evolution. In some species ... [more ▼]

Paedomorphosis, in which individuals retain ancestral characteristics in the adult stage, is widespread in newts and salamanders and is suspected to play an important role in evolution. In some species, paedomorphosis is facultative with some individuals forgoing metamorphosis. Optimality models have been proposed to explain the maintenance of this polymorphism, but require the integration of reproductive patterns into the models. We investigated the frequencies of inbreeding and outbreeding in two syntopic heterochronic morphs of the Alpine newt. The two morphs are sexually compatible: encounters between and within morphs were equally successful in terms of spermatophore transfer. Behavioural observations were in agreement with the sexual compatibility observed. Nevertheless, paedomorphic males displayed to females less frequently than metamorphic males. The two morphs differ largely on the basis of sexual secondary characteristics, but the majority of these traits did not affect mating success. Because of the large flow of genes between the two heterochronic morphs and because of the absence of spatial and temporal isolation, these results do not support sympatric speciation models, but are in favour of the maintenance of polymorphism in natural populations. (C) 2001 The Association for the Study of Animal Behaviour. [less ▲]

Detailed reference viewed: 155 (20 ULg)
Full Text
Peer Reviewed
See detailInhibition of protein phosphatase PP1 in GH3B6, but not in GH3 cells, activates the MEK/ERK/c-fos pathway and the human prolactin promoter, involving the coactivator CPB/p300
Manfroid, Isabelle ULg; Martial, Joseph ULg; Muller, Marc ULg

in Molecular Endocrinology (Baltimore, Md.) (2001), 15(4), 625-37

The human (hPRL) PRL gene proximal promoter (-164/+15) is the target for numerous signal transduction pathways involving protein kinases. The inhibitor of Ser/Thr-protein phosphatases okadaic acid (OA ... [more ▼]

The human (hPRL) PRL gene proximal promoter (-164/+15) is the target for numerous signal transduction pathways involving protein kinases. The inhibitor of Ser/Thr-protein phosphatases okadaic acid (OA) was shown to induce this promoter in rat pituitary GH3B6 through a synergism between increased amounts of the ubiquitous factor AP-1 and the pituitary-specific factor Pit-1. Here we show that this activation results mainly from transcriptional stimulation of the c-fos promoter leading to increased AP-1 activity. We report the surprising absence of the hPRL and c-fos promoter stimulation by OA in GH3 cells, closely related to GH3B6 cells, and we use this discrepancy to dissect the precise mechanism of action. c-fos gene activation involves the mitogen-activated kinase (MAPK)-ternary complex factor (TCF) pathway and can be obtained by expressing active V12ras in both cell lines. We show that OA acts by inhibiting protein phosphatase PP1, thereby protecting MAPK kinase (MEK)1/2 and/or a MEK1/2-kinase from dephosphorylation. PP1 inhibition of MEK activation by V12ras does not occur in GH3 cells, indicating that a distinct, PP1-sensitive phosphorylation site is used in GH3B6 cells to activate the TCF pathway in GH3B6 cells. Finally, we show that the synergistic OA activation of the hPRL promoter by Pit-1 and AP-1 is independent of the Pit-1 transactivation domain and is mediated by the general coactivator (CRE-binding protein)-binding protein (CBP)/p300. [less ▲]

Detailed reference viewed: 15 (2 ULg)
See detailIncinérateurs, crise dioxine et risques sanitaires pour la population belge
Bernard; Fierens; Mairesse et al

in Bulletin de la Classe des Sciences. Académie Royale de Belgique (2001), 1-6

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detail« En quête d’un nouveau droit du travail »
Florence, Eric ULg

in Revue Bibliographique de Sinologie = Review of Bibliography in Sinology (2001), Vol. XIV

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailGénéralisation de l'apprentissage d'une langue moderne à l'école primaire ; première analyse de la situation
Straeten, M.; Blondin, Christiane ULg

in Point sur la Recherche en Education (Le) (2001), 19

Detailed reference viewed: 43 (0 ULg)
Full Text
Peer Reviewed
See detailCompte rendu de Frédéric Barbier, Histoire du livre
Durand, Pascal ULg

in Bulletin des Bibliothèques de France (2001), 46/4

Detailed reference viewed: 133 (16 ULg)
Full Text
Peer Reviewed
See detailBinding and multiple instantiation in a distributed network of spiking nodes
Sougné, Jacques ULg

in Connection Science (2001), 13(2), 99-126

An implementation of a distributed connectionist network of spiking neuron-like elements is presented. Spiking nodes fire at a precise moment and transmit their activation, with particular strenghts and ... [more ▼]

An implementation of a distributed connectionist network of spiking neuron-like elements is presented. Spiking nodes fire at a precise moment and transmit their activation, with particular strenghts and delays, to nodes connected to them. Wen the potential of the node reaches a particular threshold, it emits a spike. Thereafter, the potential is reset to a resting value. The receiving nodes accumulate potential, but also, slowly lose their potential through decay. As with real neurons, after firing there is a short refractory period during which the node will be completely insensitive to incoming signals, after which its sensitivity will slowly increase. Precise timing properties are used to represent symbols in a distributed manner and also to solve the problems of variable binding and multiple instantiation. Several predictions about human short-term memory, predicate processing, complex reasoning and multiple instantiation arise from this model. This network shows how symbolic processing can be achieved using neurobiologically and psychologically plausible mechanisms that also have the advantages of generalization and noise tolerance found in connectionist networks. [less ▲]

Detailed reference viewed: 41 (5 ULg)
Full Text
Peer Reviewed
See detailCrystallographic analysis of family 11 endo-beta-1,4-xylanase Xyl1 from Streptomyces sp. S38.
Wouters, J.; Georis, J.; Engher, D. et al

in Acta Crystallographica Section D-Biological Crystallography (2001), 57(Pt 12), 1813-9

Family 11 endo-beta-1,4-xylanases degrade xylan, the main constituent of plant hemicelluloses, and have many potential uses in biotechnology. The structure of Xyl1, a family 11 endo-xylanase from ... [more ▼]

Family 11 endo-beta-1,4-xylanases degrade xylan, the main constituent of plant hemicelluloses, and have many potential uses in biotechnology. The structure of Xyl1, a family 11 endo-xylanase from Streptomyces sp. S38, has been solved. The protein crystallized from ammonium sulfate in the trigonal space group P321, with unit-cell parameters a = b = 71.49, c = 130.30 A, gamma = 120.0 degrees. The structure was solved at 2.0 A by X-ray crystallography using the molecular-replacement method and refined to a final R factor of 18.5% (R(free) = 26.9%). Xyl1 has the overall fold characteristic of family 11 xylanases, with two highly twisted beta-sheets defining a long cleft containing the two catalytic residues Glu87 and Glu177. [less ▲]

Detailed reference viewed: 30 (9 ULg)
Full Text
Peer Reviewed
See detailErosion of Deccan Traps determined by river geochemistry: impact on the global climate and the Sr-87/Sr-86 ratio of seawater
Dessert, C.; Dupre, B.; François, Louis ULg et al

in Earth And Planetary Science Letters (2001), 188(3-4), 459-474

The impact of the Deccan Traps on chemical weathering and atmospheric CO2 consumption on Earth is evaluated based on the study of major elements, strontium and Sr-87/Sr-86 isotopic ratios of the main ... [more ▼]

The impact of the Deccan Traps on chemical weathering and atmospheric CO2 consumption on Earth is evaluated based on the study of major elements, strontium and Sr-87/Sr-86 isotopic ratios of the main rivers flowing through the traps, using a numerical model which describes the coupled evolution of the chemical cycles of carbon. alkalinity and strontium and allows one to compute the variations in atmospheric pCO(2), mean global temperature and the Sr-87/Sr-86 isotopic ratio of seawater, in response to Deccan trap emplacement. The results suggest that the rate of chemical weathering of Deccan Traps (21-63 t/km(2)/yr) and associated atmospheric CO consumption (0.58-2.54 x 10(6) mol C/km(2)/yr) are relatively high compared to those linked to other basaltic regions. Our results on the Deccan and available data from other basaltic regions show that runoff and temperature are the two main parameters which control the rate of CO2 consumption during weathering of basalts, according to the relationship: f = R-f x C(0)exp[-Ea/R(1/T-1/298)] where f is the specific CO2 consumption rate (mol/km(2)/yr), R-f is runoff (mm/yr), C-0 is a constant (= 1764 mu mol/l), Ea represents an apparent activation energy for basalt weathering (with a value of 42.3 kJ/mol determined in the present study), R is the gas constant and T is the absolute temperature (K-o). Modelling results show that emplacement and weathering of Deccan Traps basalts played an important role in the geochemical cycles of carbon and strontium. In particular, the traps led to a change in weathering rate of both carbonates and silicates, in carbonate deposition on seafloor, in Sr isotopic composition of the riverine flux and hence a change in marine Sr isotopic composition. As a result, Deccan Traps emplacement was responsible for a strong increase of atmospheric pCO(2) by 1050 ppmv followed by a new steady-state pCO(2) lower than that in pre-Deccan times by 57 ppmv, implying that pre-industrial atmospheric pCO(2) would have been 20% higher in the absence of Deccan basalts. pCO(2) evolution was accompanied by a rapid warming of 4 degreesC, followed after 1 Myr by a global cooling of 0.55 degreesC. During the warming phase, continental silicate weathering is increased globally. Since weathering of continental silicate rocks provides radiogenic Sr to the ocean, the model predicts a peak in the Sr-87/Sr-86 ratio of seawater following the Deccan Traps emplacement. The amplitude and duration of this spike in the Sr isotopic signal are comparable to those observed at the Cretaceous-Tertiary boundary. The results of this study demonstrate the important control exerted by the emplacement and weathering of large basaltic provinces on the geochemical and climatic changes on Earth. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 111 (1 ULg)
Full Text
See detailLa sémiologie selon L.J. Prieto
Badir, Sémir ULg

in Linx (2001), 44

Detailed reference viewed: 31 (0 ULg)
Peer Reviewed
See detailCharge critique et longueur de flambement des éléments à inertie variable et aux conditions d'extrémité élastiques
Latteur, Pierre ULg

in revue scientifique des Instituts supérieurs industriels francophones de Belgique (2001), 15

Detailed reference viewed: 146 (2 ULg)
Peer Reviewed
See detailmarchés publics et économie sociale: l'union impossible?
Navez, Françoise ULg; Durviaux, Ann-Lawrence ULg

in Actualités du Droit (2001), 2

L'article envisage la question de l'introduction de considérations sociales dans les marchés publics: les obstacles et la technique de la clause sociale.

Detailed reference viewed: 168 (24 ULg)
Full Text
See detailPrésentation
Badir, Sémir ULg; Arrivé, Michel

in Linx (2001), 44

Detailed reference viewed: 16 (1 ULg)
Full Text
See detailLes mélèzes
Rondeux, Jacques ULg; Pauwels, D.

in Fiche technique (2001)

Detailed reference viewed: 13 (5 ULg)
Full Text
See detailConsidérations sur le taux du ressort lorsque les bases de détermination de la valeur du litige font défaut
Boularbah, Hakim ULg

in Revue de Droit Judiciaire et de la Preuve = Tijdschrift voor Procesrecht en Bewijsrecht (2001)

Detailed reference viewed: 48 (0 ULg)
Full Text
Peer Reviewed
See detailMercury porosimetry: applicability of the buckling-intrusion mechanism to low-density xerogels
Alié, Christelle ULg; Pirard, René ULg; Pirard, Jean-Paul ULg

in Journal of Non-Crystalline Solids (2001), 292(1-3), 138-149

Mineral materials can be either crushed or invaded by mercury during mercury porosimetry experiments. It has been shown here that many low-density xerogels exhibit the two volume variation mechanisms ... [more ▼]

Mineral materials can be either crushed or invaded by mercury during mercury porosimetry experiments. It has been shown here that many low-density xerogels exhibit the two volume variation mechanisms successively, compaction followed by intrusion. when submitted to mercury porosimetry and that a unimodal pore size distribution can be obtained by applying Pirard's collapse model below the pressure of transition P-1 and Washburn's intrusion theory above P-t. To confirm the validity of the use of the buckling law, one low-density xerogel was wrapped in a tight membrane (intrusion is prevented and the sample is crushed during the whole porosimetry experiment). The analysis of the mercury porosimetry data of the wrapped sample by the buckling law leads to a continuous unimodal distribution similar to the distribution of the unwrapped sample obtained by applying the buckling law below P-t and the intrusion law above P-t. The position of P-t is characteristic of the tested material: when submitted to mercury pressure. aerogels and low-density xerogels only collapse in case of very small aggregates whereas they are crushed and then intruded in case of larger silica aggregates. The fact that compacted slabs of monodisperse non-aggregated silica spheres (of the same size range as the xerogels and aerogels) show only intrusion during mercury porosimetry experiments implies that the particles need to be aggregated so that the compaction mechanism takes place. The position of the change of mechanism from crushing to intrusion is not directly related to the size of the elementary particles but is linked to the size of the aggregates of silica particles. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 38 (4 ULg)
Full Text
Peer Reviewed
See detailSecondary fabrics revealed by remanence anisotropy: methodological study and examples from plutonic rocks
Trindade, Ricardo I.F.; Bouchez, Jean-Luc; Bolle, Olivier ULg et al

in Geophysical Journal International (2001), 147(2), 310-318

Detailed reference viewed: 14 (2 ULg)
Peer Reviewed
See detailOptimisation des arcs paraboliques et en chaînette – aide à la conception sur base d'indicateurs morphologiques
Latteur, Pierre ULg; Samyn, Philippe; De Wilde, Patrick

in Revue Française de Génie Civil (2001), 5

Detailed reference viewed: 33 (0 ULg)
Full Text
Peer Reviewed
See detailKinetics of light emission and oxygen consumption by bioluminescent bacteria.
Bourgeois, J. J.; Sluse, Francis ULg; Baguet, F. et al

in Journal of Bioenergetics & Biomembranes (2001), 33

Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a ... [more ▼]

Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium phosphoreum incubated on respiratory substrates chosen for their different reducing power. The general patterns of the luminescence time courses are different among species but not among substrates. During steady-state conditions, substrates, which are less reduced than glycerol, have, paradoxally, a better luminescence efficiency. Oxygen consumption by luciferase has been evaluated to be approximately 17% of the total respiration. Luciferase is a regulatory enzyme presenting a positive cooperative effect with oxygen and its affinity for this final electron acceptor is about 4-5 times higher than the one of cytochrome oxidase. The apparent Michaelis constant for luciferase has been evaluated to be in the range of 20 to 65 nM O2. When O2 concentrations are as low as 10 nM, luminescence can still be detected; this means that above this concentration, strict anaerobiosis does not exist. By n-butyl malonate titration, it was clearly shown that electrons enter the luciferase pathway only when the cytochrome pathway is saturated. It is suggested that, in bioluminescent bacteria, luciferase acts as a free-energy dissipating valve when anabolic processes (biomass production) are impaired. [less ▲]

Detailed reference viewed: 38 (1 ULg)
Full Text
Peer Reviewed
See detailc-JUN gene induction and AP-1 activity is regulated by a JNK-dependent pathway in hypoxic HepG2 cells.
Minet, E.; Michel, G.; Mottet, Denis ULg et al

in Experimental Cell Research (2001), 265(1), 114-24

Hypoxia is an important pathophysiological stress that occurs during blood vessel injuries and tumor growth. It is now well documented that hypoxia leads to the activation of several transcription factors ... [more ▼]

Hypoxia is an important pathophysiological stress that occurs during blood vessel injuries and tumor growth. It is now well documented that hypoxia leads to the activation of several transcription factors which participate in the adaptive response of the cells to hypoxia. Among these transcription factors, AP-1 is rapidly activated by hypoxia and triggers bFGF, VEGF, and tyrosine hydroxylase gene expression. However, the mechanisms of AP-1 activation by hypoxia are not well understood. In this report, we studied the events leading to AP-1 activation in hypoxia. We found that c-jun protein accumulates in hypoxic HepG2 cells. This overexpression is concomitant with c-jun phosphorylation and JNK activation. Moreover, we showed that AP-1 is transcriptionally active. We also observed that AP-1 transcriptional activity is inhibited by a MEKK1 dominant negative mutant. Moreover, the MEKK1 dominant negative mutant as well as deletion of the AP-1 binding sites within the c-jun promoter inhibited the c-jun promoter activation by hypoxia. All together, these results indicate that, in hypoxic HepG2 cells, AP-1 is activated through a JNK-dependent pathway and that it is involved in the regulation of the c-jun promoter, inducing a positive feedback loop on AP-1 activation via c-jun overexpression. [less ▲]

Detailed reference viewed: 62 (3 ULg)
Peer Reviewed
See detailHpa Axis Dysfunction in Major Depression: Relationship to 5-Ht(1a) Receptor Activity
Pitchot, William ULg; Herrera, C.; Ansseau, Marc ULg

in Neuropsychobiology (2001), 44(2), 74-7

Major depression is associated with a dysfunction of the serotonergic activity and the hypothalamic-pituitary-adrenal (HPA) axis. Moreover, a reciprocal relationship between the serotonergic and HPA axis ... [more ▼]

Major depression is associated with a dysfunction of the serotonergic activity and the hypothalamic-pituitary-adrenal (HPA) axis. Moreover, a reciprocal relationship between the serotonergic and HPA axis systems has been hypothesized. The purpose of the present study was to assess the relationship between sensitivity of 5-HT(1A) receptors as measured by hormonal (ACTH, cortisol and PRL) and temperature responses to flesinoxan and HPA axis activity as measured by the dexamethasone suppression test. The sample included 21 inpatients with major depression. Dexamethasone nonsuppressors exhibited lower ACTH responses to flesinoxan as compared with dexamethasone suppressors. The results showed that a dysfunction in 5-HT(1A) receptor activity could be due to a hypersecretion of cortisol. [less ▲]

Detailed reference viewed: 17 (1 ULg)
See detailHelping children to learn languages (Aider son enfant à apprendre les langues)
Blondin, Christiane ULg

in European Parents Association - Info Bulletin (2001), (Hiver 2001), 11-16

Detailed reference viewed: 31 (3 ULg)
Full Text
Peer Reviewed
See detailPreparation of low-density xerogels by incorporation of additives during synthesis
Alié, Christelle ULg; Ferauche, Fabrice; Pirard, René ULg et al

in Journal of Non-Crystalline Solids (2001), 289(1-3), 88-96

Low-density xerogels were prepared by incorporation of an additive to alcogels prior to gelation. The additives studied are 3-(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3 ... [more ▼]

Low-density xerogels were prepared by incorporation of an additive to alcogels prior to gelation. The additives studied are 3-(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3-aminopropyltrimethoxysilane (AMS), propyltrimethoxysilane (PMS), tetramethylorthosilicate (TMOS) and 3-aminopropyltriethoxysilane (AES) using tetraethylorthosilicate (TEOS) as main silica precursor. Samples were also prepared with EDAS as additive and TMOS as main silica reagent. When the additive contains methoxy groups, it reacts first, forms nuclei on which the main reagent TEOS reacts to form the silica particles. The nucleation mechanism by the additive occurs only in case of a difference of reactivity between additive and main silica precursor. The other group of the additive (amine, alkyl group, ...) influences only the gelation time. In case of ethoxy groups (series AES/TEOS) or methoxy groups (series EDAS/TMOS) for both additive and main reagent, there is no nucleation by the additive. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 20 (3 ULg)
Full Text
Peer Reviewed
See detailLe contrôle de la constitutionnalité des lois aux Pays-Bas
Kohl, Benoît ULg

in Chroniques de Droit Public = Publiekrechtelijke Kronieken (2001)

Detailed reference viewed: 42 (2 ULg)
Full Text
Peer Reviewed
See detailL'impact de la readaptation cardiaque sur la qualite de vie apres un pontage aorto-coronaire : Une etude belge
Etienne, Anne-Marie ULg; Fontaine, Ovide ULg; Dupuis, Gilles et al

in Revue Francophone de Clinique Comportementale et Cognitive (2001), 6(2), 15-25

The aim of this study was to measure the quality of life of coronary artery bypass surgery patients enrolled or not in a rehabilitation program. The study was prospective and longitudinal and used ... [more ▼]

The aim of this study was to measure the quality of life of coronary artery bypass surgery patients enrolled or not in a rehabilitation program. The study was prospective and longitudinal and used patients’ self selection as the allocation strategy to constitute the two groups: rehabilitation and no-rehabilitation. Forty first coronary bypass surgery patients (38 men and 2 women, mean age 59 +/-8) were recruited from four hospitals in a French area of Belgium. Patients were evaluated before and after surgery with: the Quality of Life Systemic Inventory (QLSI), the Beck Depression Inventory, the State Anxiety Inventory, a somatic symptoms questionnaire, a physical activities questionnaire, and a medical questionnaire. Both groups improved on the QLSI global score of quality of life, but the effect size (eta squared) was greater in the rehab group (0.5) compared to the no rehab group (0.25) [less ▲]

Detailed reference viewed: 438 (7 ULg)
Full Text
See detailLe dispositif d'évaluation externe en Communauté française de Belgique
Lafontaine, Dominique ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2001), (7-8), 195-218

Detailed reference viewed: 126 (10 ULg)
Full Text
Peer Reviewed
See detailModified hormonal balance in rooting-recalcitrant rac mutant tobacco shoots
Faivre-Rampant, Odile; Kevers, Claire ULg; Dommes, Jacques ULg et al

in Plant Biosystems (2001), 135(1), 85-93

The rooting-recalcitrant rac tobacco mutant has been multiplied in vitro via outgrowth of axillary buds in parallel to the DS wild-type. The mutant shoots grew at a lower rate and did not root whatever ... [more ▼]

The rooting-recalcitrant rac tobacco mutant has been multiplied in vitro via outgrowth of axillary buds in parallel to the DS wild-type. The mutant shoots grew at a lower rate and did not root whatever the treatments, whereas the wild-type shoots rooted spontaneously during the culture cycle without auxin treatment. The mutant and wild-type shoots showed similar peroxidase variations along the culture cycle (21 days) but with higher levels of activity for the rac mutant: minimum peroxidase activity occurrey at day 14 in whole shoots of both tobacco genotypes, but already at day 7 in the basal parts of the stems (where roots appear) of the wild-type tobacco, while it was delayed in the mutant. Free and conjugated auxin and polyamine levels were also determined in whole shoots and basal parts of the stems. The rac mutant was characterised by higher auxin and polyamine contents. A peak of auxins and polyamines appeared at day 14 in the whole shoots whatever the tobacco genotype. This peak was delayed in the basal parts of the rac stems compared to the wild-type ones. The mutant shoots contained higher levels of benzyladenine and isopentenyladenosine at the end of the culture cycle, whereas zeatin riboside was more abundant in wild-type shoots. In response to increasing concentrations of indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA), only the wild-type shoots responded by an increase in growth rate followed by inhibition at high concentrations. The rac shoot responses were very low or nonexistent. Peroxidase activity was also measured in E basal parts of tobacco stems grown in the presence of IBA. Results suggest growth inhibition related to auxin accumulation, possibly combined with elevated putrescine content. Second, rooting induction seems to take place in both tobacco genotypes; however, the process of root formation is blocked in the mutant. The lack of initiation and expression phases of rooting in relation to auxin content in the mutant is discussed. [less ▲]

Detailed reference viewed: 72 (5 ULg)
Full Text
Peer Reviewed
See detailVingt ans après... Regards d'un didacticien sur l'enseignement de la littérature française de Belgique
Dumortier, Jean-Louis ULg

in Textyles : Revue des Lettres Belges de Langue Française (2001), 19

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailThe use of additives to prepare low-density xerogels
Alié, Christelle ULg; Pirard, René ULg; Lecloux, A. J. et al

in Journal of Non-Crystalline Solids (2001), 285

Detailed reference viewed: 16 (6 ULg)
Peer Reviewed
See detailLe développement de partenariats multilingues. Une expérience aux dimensions européennes en Communauté française de Belgique
Campolini, L.; Jucquois, G.; Flamini, F. et al

in Annales d'Etudes Européennes de l'Université Catholique de Louvain (2001), 5

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailComparison between the GIRBAS Scale and the Acoustic and Aerodynamic Measures Provided by EVA for the Assessment of Dysphonia following Unilateral Vocal Fold Paralysis.
Morsomme, Dominique ULg; Jamart, Jacques; Wery, Carine et al

in Folia Phoniatrica et Logopaedica (2001), 53(6), 317-25

The aim of this study is to establish relevant objective parameters for evaluating dysphonia following unilateral vocal fold paralysis. To do so, the study compares objective and perceptual voice measures ... [more ▼]

The aim of this study is to establish relevant objective parameters for evaluating dysphonia following unilateral vocal fold paralysis. To do so, the study compares objective and perceptual voice measures. The objective measures were obtained using a voice analysis software (Evaluation Vocale Assistee), whereas the perceptual measures were established with the GIRBAS Scale (grade, instability, roughness, breathiness, asthenia, and strain). All measurements were performed on 40 voice samples: 28 dysphonic subjects with unilateral laryngeal paralysis, and 12 control subjects. The intra- and inter-judge agreements were fairly good, at least for control subjects. The six GIRBAS measures obtained from the pathological voices were higher than those from the control voices (p < 0.001) and the correlation between both groups was good. Grade, breathiness and asthenia correlated with the objective parameters that express the aperiodicity of the phonatory signal (p < 0.01), namely, the coefficient of variability of the fundamental frequency, the coefficient of variability of intensity, and jitter. Our findings suggest that the perceptual reality of laryngeal paralysis-induced dysphonia depends more on grade, breathiness and asthenia than it does on roughness or instability. [less ▲]

Detailed reference viewed: 65 (8 ULg)
Full Text
Peer Reviewed
See detailPleiotropic Mutants Hypersensitive to Heavy Metals and to Oxidative Stress in Chlamydomonas Reinhardtii
Hanikenne, Marc ULg; Matagne, René F.; Loppes, Roland ULg

in FEMS Microbiology Letters (2001), 196(2),

Insertional mutagenesis was used in Chlamydomonas reinhardtii to isolate original mutants hypersensitive to multiple drugs and physical agents. Out of 5200 transformants analyzed, 13 mutants belonging to ... [more ▼]

Insertional mutagenesis was used in Chlamydomonas reinhardtii to isolate original mutants hypersensitive to multiple drugs and physical agents. Out of 5200 transformants analyzed, 13 mutants belonging to seven phenotypic classes were isolated. Five were exclusively sensitive to cadmium and represented two loci. The other mutants were pleiotropic and presented a cross sensitivity to several (2--6) of the following agents: cadmium, copper, lead, paraquat, hydrogen peroxide, UVC and light. In all mutants analyzed, the hypersensitive phenotype was most probably due to a single mutational event. The sensitivity of several pleiotropic mutants to a broad range of physical and chemical agents suggests that the disrupted genes are involved in multiple stress responses. [less ▲]

Detailed reference viewed: 46 (2 ULg)
Full Text
Peer Reviewed
See detailIntensive and Prolonged Health Promotion Strategy May Increase Awareness of Osteoporosis among Postmenopausal Women
Tellier, V.; De Maeseneer, J.; De Prins, L. et al

in Osteoporosis International (2001), 12(2), 131-5

The aim of the study was to measure the results of a 15-year health promotion strategy towards osteoporosis, in an urban community of subjects over 45 years old, in terms of osteoporosis awareness and ... [more ▼]

The aim of the study was to measure the results of a 15-year health promotion strategy towards osteoporosis, in an urban community of subjects over 45 years old, in terms of osteoporosis awareness and handling. To this end an ancillary study to a large survey of the Belgian population's self-perceived health status was carried out. A rectangular sample of 4800 individuals over 45 years old was randomly selected in two Belgian cities, among the affiliates of the two main health insurance providers. One of the cities (Liege) had been, since the early 1980s, the target of a constant health promotion strategy, directed to both the medical community and the general population, aimed at increasing osteoporosis awareness in women after the menopause. During the same period, no particular steps were taken in the other city (Aalst) to increase osteoporosis awareness in the community. In our study, the participants were asked to spontaneously report any chronic, serious and/or severe disorders that they had been suffering from, for at least 6 months, during the previous 12 months. They also provided a list of drugs they were taking at the time of the survey. Osteoporosis was reported to be a disease affecting 1.5% of men in Aalst and 1.3% of men in Liege (p = 0.61). For women, osteoporosis was reported to be present in 4.8% in Aalst and 10.8% in Liege (p<0.001). Self-reporting of osteoporosis prevalence in Liege was statistically significantly higher in women aged 45-64 years, 65-74 years or over 75 years (p<0.001). Obesity, alcohol consumption or physical activity were equally distributed between women from Liege and Aalst. Prescription drugs used for osteoporosis had been delivered to a similar proportion of men in Aalst and Liege. In women, a statistically significant difference in these prescription drugs was observed between Liege and Aalst, both for the overall population (p<0.001) and in each of the age classes (p<0.001 for 45-64 years and 65-74 years; p<0.009 for over 75 years). A continuous long-term health promotion strategy, directed toward both physicians and the general population, thus appears to increase awareness about osteoporosis in women over 45 years and/or in the medical community. This is reflected by an increase in self-reported prevalence of osteoporosis and in the prescription of drugs aimed at prevention and treatment of this disorder. Whether these observations reflect an appropriate diagnosis and a proper handling of the disease remains to be evaluated by objective diagnostic tools such as bone densitometry and by an evaluation of the effectiveness of prescription practices in postmenopausal women. [less ▲]

Detailed reference viewed: 55 (1 ULg)
Full Text
Peer Reviewed
See detailBelgian normative data of the temperament and character inventory
Hansenne, Michel ULg; Le Bon, O.; Gauthier, Audrey ULg et al

in European Journal of Psychological Assessment (2001), 17(1), 56-62

The Temperament and Character Inventory (TCI) is a 226-item self-questionnaire developed to assess the seven dimensions of personality described by Cloninger and his colleagues. Normative data from 322 ... [more ▼]

The Temperament and Character Inventory (TCI) is a 226-item self-questionnaire developed to assess the seven dimensions of personality described by Cloninger and his colleagues. Normative data from 322 representative French-speaking subjects from Belgium are presented and the psychometric properties are discussed. Mean scores of temperament dimensions were appreciably different from those published by Cloninger. In our sample, novelty seeking and self-transcendence scores were lower and harm avoidance scores were higher compared to US norms. The other dimensions were almost identical. The factorial analysis showed that the hypothesized factor structure of temperament and character dimensions was almost confirmed. The present study also confirmed that the TCI scales were weakly related among themselves. The relationships were consistent with those reported by previous reports. Gender differences were also found for different dimensions. [less ▲]

Detailed reference viewed: 77 (13 ULg)
Full Text
Peer Reviewed
See detailMicrophase separation at the surface of block copolymers, as studied with atomic force microscopy
Rasmont, A.; Leclère, Philippe; Doneux, C. et al

in Colloids and Surfaces B : Biointerfaces (2000), 19(4), 381-395

Atomic force microscopy (AFM) is used to study the phase separation process occurring in block copolymers in the solid state. The simultaneous measurement of the amplitude and the phase of the oscillating ... [more ▼]

Atomic force microscopy (AFM) is used to study the phase separation process occurring in block copolymers in the solid state. The simultaneous measurement of the amplitude and the phase of the oscillating cantilever in the tapping mode operation provides the surface topography along with the cartography of the microdomains of different mechanical properties. This technique thus allows to characterize the size and shape of those microdomains and their organization at the surface (e.g. cubic lattice spheres, hexagonal lattice of cylinders, or lamellae). In this study, a series of symmetric triblock copolymers made of a inner elastomeric sequence (poly(butadiene) or poly(alkylacrylate)) and two outer thermoplastic sequences (poly(methylmethacrylate)) is analyzed by AFM in the tapping mode. The microphase separation and their morphology are essential factors for the potential of these materials as a new class of thermoplastic elastomers. Special attention is paid to the control of the surface morphology, as observed by AFM, by the molecular structure of the copolymers (volume ratio of the sequences, molecular weight, length of the alkyl side group) and the experimental conditions used for the sample preparation. The molecular structure of the chains is completely controlled by the synthesis, which relies on the sequential living anionic polymerization of the comonomers. The copolymers are analyzed as solvent-cast films, whose characteristics depend on the solvent used and the annealing conditions. The surface arrangement of the phase-separated elastomeric and thermoplastic microdomains observed on the AFM phase images is discussed on the basis of quantitative information provided by the statistical analysis by Fourier transform and grain size distribution calculations. [less ▲]

Detailed reference viewed: 58 (4 ULg)
Full Text
Peer Reviewed
See detailCharacterization of porous polylactide foams by image analysis and impedance spectroscopy
Maquet, Véronique; Blacher, Silvia ULg; Pirard, René ULg et al

in Langmuir (2000), 16(26), 10463-10470

The texture of highly porous polylactide foams prepared by freeze-drying of poly-L-lactide (L-PLA) and poly-DL-lactide (DL-PLA) solutions of various concentrations was investigated by two novel ... [more ▼]

The texture of highly porous polylactide foams prepared by freeze-drying of poly-L-lactide (L-PLA) and poly-DL-lactide (DL-PLA) solutions of various concentrations was investigated by two novel methodologies, image analysis and impedance spectroscopy. Image analysis of scanning electron micrographs of transverse cross-sections at two different magnifications gave information on both the macroporosity (1 mum < width 10 <mu>m) and ultramacroporosity (width > 10 mum). Impedance spectroscopy was used to investigate the transport properties of the three-dimensional porous matrices by measurement of ionic conduction. Image analysis showed that: (a) the macroporosity, which mainly contributes to porosity, is independent of the sample composition; (b) when the concentration of the polymer solution is increased, the density of the ultramacropores decreases and their average diameter increases; (c) the distribution of the ultramacropores is more homogeneous in the semicrystalline L-PLA. foams than in the amorphous DL-PLA counterparts, in which the ultramacropores tend to make clusters. The dielectric properties changed at low frequency, in relation to modifications in the ultramacroporosity. Ultramacropores of the L-PLA foams were found to be more open and more sensitive to the concentration of the polymer solutions compared with DL-PLA. Expectedly, the mechanical properties of the PLA foams changed with the structure of the ultramacroporous network. These results encourage further investigations on the texture of porous supports, to collect pertinent information on the physical macro- and ultramacroenvironment in which cells will reside. [less ▲]

Detailed reference viewed: 44 (14 ULg)
Full Text
Peer Reviewed
See detailPeripheral Nerve Regeneration Using Bioresorbable Macroporous Polylactide Scaffolds
Maquet, Véronique; Martin, Didier ULg; Malgrange, Brigitte ULg et al

in Journal of Biomedical Materials Research, Part A (2000), 52(4), 639-51

The ability of DRG-derived neurons to survive and attach onto macroporous polylactide (PLA) foams was assessed in vitro. The foams were fabricated using a thermally induced polymer-solvent phase ... [more ▼]

The ability of DRG-derived neurons to survive and attach onto macroporous polylactide (PLA) foams was assessed in vitro. The foams were fabricated using a thermally induced polymer-solvent phase separation. Two types of pore structures, namely oriented or interconnected pores, can be produced, depending on the mechanism of phase separation, which in turn can be predicted by the thermodynamics of the polymer-solvent pair. Coating of the porous foams with polyvinylalcohol (PVA) considerably improved the wettability of the foams and allowed for cell culture. The in vitro biocompatibility of the PVA-coated supports was demonstrated by measuring cell viability and neuritogenesis. Microscopic observations of the cells seeded onto the polymer foams showed that the interconnected pore networks were more favorable to cell attachment than the anisotropic ones. The capacity of highly oriented foams to support in vivo peripheral nerve regeneration was studied in rats. A sciatic nerve gap of 5-mm length was bridged with a polymer implant showing macrotubes of 100 microm diameter. At 4 weeks postoperatively, the polymer implant was still present. It was well integrated and had restored an anatomic continuity. An abundant cell migration was observed at the outer surface of the polymer implant, but not within the macrotubes. This dense cellular microenvironment was found to be favorable for axogenesis. [less ▲]

Detailed reference viewed: 45 (9 ULg)
Full Text
Peer Reviewed
See detailType Iv Collagen Induces Matrix Metalloproteinase 2 Activation in Ht1080 Fibrosarcoma Cells
Maquoi, Erik ULg; Frankenne, F.; Noël, Agnès ULg et al

in Experimental Cell Research (2000), 261(2), 348-59

Matrix metalloproteinase 2 (MMP-2) activation has been described as a "master switch" which triggers tumor spread and metastatic progression. We show here that type IV collagen, a major component of ... [more ▼]

Matrix metalloproteinase 2 (MMP-2) activation has been described as a "master switch" which triggers tumor spread and metastatic progression. We show here that type IV collagen, a major component of basement membranes, promotes MMP-2 activation by HT1080 cells. When plated on plastic, HT1080 cells constitutively processed the 66-kDa pro-MMP-2 into a 62-kDa intermediate activated form, most probably through a membrane type (MT) 1 MMP-dependent mechanism. In the presence of type IV collagen, part of this intermediate form was further processed to fully activated 59-kDa MMP-2. This activation was prevented by tissue inhibitor of MMP (TIMP)-2 and a broad-spectrum hydroxamic acid-based synthetic MMP inhibitor (GI129471). Type IV collagen-mediated pro-MMP-2 activation did not involve either a transcriptional modulation of MMP-2, MT1-MMP, or TIMP-2 expression nor any alteration of MT1-MMP protein synthesis or processing. An inverse relationship between MMP-2 activation and the concentration of secreted TIMP-2 was observed. This is consistent with our previous report that TIMP-2 degradation is probably linked to the MT1-MMP-dependent MMP-2 activation mechanism. Because invasive tumor cells must breach basement membranes at different steps of the metastatic dissemination, the ability of HT1080 cells to activate pro-MMP-2 in the presence of type IV collagen might represent a key regulatory mechanism for the acquisition of an invasive potential. [less ▲]

Detailed reference viewed: 47 (0 ULg)
Full Text
Peer Reviewed
See detailWater-soluble complexes formed by sodium poly(4-styrenesulfonate) and a poly(2-vinylpyridinium)-block-poly(ethyleneoxide) copolymer
Gohy, Jean-François; Varshney, Sunil K; Antoun, Said et al

in Macromolecules (2000), 33(25), 9298-9305

Formation of interpolyelectrolyte complexes (IPEC) between sodium poly(4-styrenesulfonate) (PSSO3Na) and a diblock copolymer consisting of a protonated poly(2-vinylpyridine) (P2VP) block and a neutral ... [more ▼]

Formation of interpolyelectrolyte complexes (IPEC) between sodium poly(4-styrenesulfonate) (PSSO3Na) and a diblock copolymer consisting of a protonated poly(2-vinylpyridine) (P2VP) block and a neutral poly(ethylene oxide) (PEO) one was investigated in water. The main experimental variables were the molecular weight (MW) of the PSSO3Na polyanion, the molar ratio of the cationic and anionic species (i.e., the stoichiometry of the complex), and the pH of the aqueous solution. It was observed that (i) the interpolyelectrolyte complexes self-assemble at least at low pH into the core of monodisperse spherical micelles surrounded by a corona of PEO blocks and possibly of uncomplexed PSSO3Na segments, (ii) their stability depends on the pH in relation to the degree of protonation of the P2VP block, whatever the MW of the PSSO3Na polyanion and the cation/anion stoichiometry, (iii) the complexes are dissociated above a critical pH and are salt-sensitive, falling apart above a critical salt concentration, and (iv) a cooperative mechanism operates, which is completely reversible. [less ▲]

Detailed reference viewed: 43 (3 ULg)
Full Text
Peer Reviewed
See detailAdsorption of Poly(vinylpyrrolidone)/Surfactant(s) mixtures at the silica/water interface: a calorimetric investigation
Thibaut, Anne; Misselyn-Bauduin, Anne-Marie; Broze, Guy ULg et al

in Langmuir (2000), 16(25), 9841-9849

The adsorption of binary mixtures of poly(vinylpyrrolidone) (PVP) and sodium dodecyl sulfate (SDS) and of ternary mixture of PVP, SDS, and pentaethylene glycol monodecyl ether (C10E5) onto silica has been ... [more ▼]

The adsorption of binary mixtures of poly(vinylpyrrolidone) (PVP) and sodium dodecyl sulfate (SDS) and of ternary mixture of PVP, SDS, and pentaethylene glycol monodecyl ether (C10E5) onto silica has been studied by the measurements of the adsorbed amount of PVP and the heat of adsorption. The amount of PVP adsorbed from the binary mixtures decreases as the SDS concentration is increased, whereas the enthalpy change passes through a maximum at SDS concentration of ca. 4 g/L. The chain conformation of PVP adsorbed on silica has been estimated from the adsorption data in relation to the surfactant concentration. It changes from loops and tails to trains upon increasing SDS concentration. At SDS concentrations higher than 4 g/L, a comblike conformation is observed. The adsorption of PVP/SDS/C10E5 ternary mixtures depends on the polymer concentration regime. [less ▲]

Detailed reference viewed: 45 (1 ULg)
Full Text
Peer Reviewed
See detailSpecific Passive Immunity Reduces the Excretion of Glycoprotein E-Negative Bovine Herpesvirus Type 1 Vaccine Strain in Calves
Lemaire, Mylène; Hanon, E.; Schynts, Frédéric et al

in Vaccine (2000), 19(9-10), 1013-7

We investigated the excretion of either a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV1) vaccine strain or a conventional modified-live vaccine strain in both naive and passively immunised ... [more ▼]

We investigated the excretion of either a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV1) vaccine strain or a conventional modified-live vaccine strain in both naive and passively immunised calves. The replication of gE-negative strain was considerably reduced in the maternally immunised calves, in comparison with the non-immune calves. On the other hand, the excretion of the gE-positive conventional vaccine strain was not reduced and even seemed to be prolonged in the presence of maternal antibodies. These results suggest that BHV1 gE may play a role in virus survival in the presence of antibodies. [less ▲]

Detailed reference viewed: 21 (0 ULg)
Peer Reviewed
See detailTemporal differentiation in two strains of small rodents: a wood mouse (Apodemus sylvaticus) and an albino mouse (Mus musculus OF1)
Lejeune, Helga ULg; Huynen, Marie-Claude ULg; Ferrara, André ULg

in Behavioural Processes (2000), 52(2-3), 155-169

Wood mice (Apodemus sylvaticus) and OF1 albino mice (Mus musculus) were compared over durations ranging from 0.5 to 7 s, using the differential reinforcement of response duration schedule (DRRD) and a ... [more ▼]

Wood mice (Apodemus sylvaticus) and OF1 albino mice (Mus musculus) were compared over durations ranging from 0.5 to 7 s, using the differential reinforcement of response duration schedule (DRRD) and a 'platform' response, i.e. staying on a small platform for a specified criterion duration to be reinforced. Species-related differences were found for mean response durations, efficiency and the number of trials needed to reach a preset performance criterion. Coefficients of variation of response durations did not differ. Overall, OF1 mice needed more trials than wood mice to reach a temporal criterion. However, over 3-7 s, data from both strains almost fitted the behavioral assumptions of Scalar Timing theory. Performance of mongolian gerbils (Meriones unguiculatus) trained in a similar setting was shown for visual comparison. [less ▲]

Detailed reference viewed: 39 (8 ULg)
Full Text
Peer Reviewed
See detailPlacental growth hormone (GH), GH-binding protein, and insulin-like growth factor axis in normal, growth-retarded, and diabetic pregnancies: Correlations with fetal growth
McIntyre, H. D.; Serek, R.; Crane, D. I. et al

in Journal of Clinical Endocrinology and Metabolism (2000), 85(3), 1143-1150

We previously described significant changes in GH-binding protein (GHBP) in pathological human pregnancy. There was a substantial elevation of GHBP in cases of noninsulin-dependent diabetes mellitus and a ... [more ▼]

We previously described significant changes in GH-binding protein (GHBP) in pathological human pregnancy. There was a substantial elevation of GHBP in cases of noninsulin-dependent diabetes mellitus and a reduction in insulin-dependent diabetes mellitus. GHBP has the potential to modulate the proportion of free placental GH (PGH) and hence the impact on the maternal GH/insulin-like growth factor I (IGF-I) axis, fetal growth, and maternal glycemic status. The present study was undertaken to investigate the relationship among glycemia, GHBP, and PGH during pregnancy and to assess the impact of GHBP on the concentration of free PGH. We have extended the analysis of specimens to include measurements of GHBP, PGH, IGF-I, IGF-II, IGF-binding protein-1 (IGFBP-1), IGFSP-2, and IGFBP-3 and have related these to maternal characteristics, fetal growth, and glycemia. The simultaneous measurement of GHBP and PGH has for the first time allowed calculation of the free component of PGH and correlation of the free component to indexes of fetal growth and other endocrine markers. PGH, free PGH, IGF-I, and IGF-II were substantially decreased in IUGR at 28-30 weeks gestation (K28) and 36-38 weeks gestation (K36). The mean concentration (+/-SEM) of total PGH increased significantly from K28 to K36 (30.0 +/- 2.2 to 50.7 +/- 6.2 ng/mL; n = 40), as did the concentration of free PGH (23.4 +/- 2.3 to 43.7 +/- 6.0 ng/mL; n = 38). The mean percentage of free PGH was significantly less in IUGR than in normal subjects (67% vs. 79%; P < 0.01). Macrosomia was associated with an increase in these parameters that did not reach statistical significance. Multiple regression analysis revealed that PGH/IGF-I and IGFBP-5 account for 40% of the variance in birth weight. IGFBP-3 showed a significant correlation with IGF-I, IGF-II, and free and total PGK at K28 and K36. Noninsulin-dependent diabetes mellitus patients had a lower mean percentage of free PGH (65%; P < 0.01), and insulin-dependent diabetics had a higher mean percentage of free PGH (87%; P < 0.01) than normal subjects. Mean postprandial glucose at K28 correlated positively with PGH and free PGH (consistent with the hyperglycemic action of GH). GHBP correlated negatively with both postprandial and fasting glucose. Although GHBP correlated negatively with PGH (r = -0.52; P <.001), free PGH and total PGH correlated very closely (r = 0.98). The results are consistent with an inhibitory function for GHBP in vivo and support a critical role for placental GH and IGF-I in driving normal fetal growth. [less ▲]

Detailed reference viewed: 35 (8 ULg)
Full Text
Peer Reviewed
See detailVariations of the Ap star HDE 318107
Manfroid, Jean ULg; Mathys, G.

in Astronomy and Astrophysics (2000), 364

The rotation period of the Ap star HDE 318107 has been determined from magnetic and photometric observations obtained between August 1992 and September 1998.

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailGenotypic Characterization of Enteropathogenic Escherichia Coli (Epec) Isolated in Belgium from Dogs and Cats
Goffaux, F.; China, B.; Janssen, L. et al

in Research in Microbiology (2000), 151(10), 865-71

Enteropathogenic Escherichia coli (EPEC) are isolated from man and farm animals but also from dogs and cats. They produce typical histological lesions called 'attaching and effacing' lesions. Both plasmid ... [more ▼]

Enteropathogenic Escherichia coli (EPEC) are isolated from man and farm animals but also from dogs and cats. They produce typical histological lesions called 'attaching and effacing' lesions. Both plasmid and chromosomal elements are involved in the pathogenesis of EPEC infection. The presence of these genetic elements was investigated in 14 dog and three cat EPEC isolates. A bfpA-related gene was detected in five of the 17 isolates in association with high molecular weight plasmids, and a locus of enterocyte effacement (LEE) was present in all isolates. The LEE was inserted in the selC region in only 12% of the isolates. The eae, tir, espA and espB genes were analyzed by multiplex PCR. The results indicated the presence of those genes in the tested isolates with heterogeneity in the gene subtypes present: eae gamma-tir alpha-espA alpha-espB alpha (65%), eae beta-tir beta-espA beta-espB beta (29%), eae alpha-tir alpha-espA alpha-espB alpha (6%). Moreover, the espD gene was also present in dog and cat EPEC. The DEPEC and CEPEC form a heterogeneous group and five of them are closely related to human EPEC. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Peer Reviewed
See detailTaxonomy of stock market indices
Bonanno, G.; Vandewalle, Nicolas ULg; Mantegna, R. N.

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (2000), 62(6),

We investigate sets of financial nonredundant and nonsynchronously recorded time series. The sets are composed by a number of stock market indices located all over the world in five continents. By ... [more ▼]

We investigate sets of financial nonredundant and nonsynchronously recorded time series. The sets are composed by a number of stock market indices located all over the world in five continents. By properly selecting the time horizon of returns and by using a reference currency we find a meaningful taxonomy. The detection of such a taxonomy proves that interpretable information can be stored in a set of nonsynchronously recorded time series. [less ▲]

Detailed reference viewed: 20 (1 ULg)
Full Text
Peer Reviewed
See detailBone Sialoprotein Mrna and Protein Expression in Human Multiple Myeloma Cell Lines and Patients
Bellahcene, Akeila ULg; Van Riet, I.; de Greef, C. et al

in British Journal of Haematology (2000), 111(4), 1118-21

Bone sialoprotein (BSP) is a glycoprotein essentially found in mineralizing connective tissues. We have recently demonstrated that BSP is ectopically expressed by carcinomas that metastasize to bone with ... [more ▼]

Bone sialoprotein (BSP) is a glycoprotein essentially found in mineralizing connective tissues. We have recently demonstrated that BSP is ectopically expressed by carcinomas that metastasize to bone with high frequency. Multiple myeloma (MM) is characterized by the localization of tumour plasma cells in the bone marrow. In this study, BSP expression was evaluated in human myeloma cell lines and in bone marrow aspirates and one ascites fluid from MM patients. BSP was detectable in conditioned media of MM cell lines. Using FACS analysis and in situ hybridization, we demonstrated that tumour cells from all MM patients and cell lines analysed express BSP at both the protein and the mRNA level. [less ▲]

Detailed reference viewed: 28 (3 ULg)
Full Text
Peer Reviewed
See detailA 295-Kda Intermediate Filament-Associated Protein in Radial Glia and Developing Muscle Cells in Vivo and in Vitro
Chanas-Sacre, Grazyna; Thiry, Marc ULg; Pirard, Sandrine et al

in Developmental Dynamics : An Official Publication of the American Association of Anatomists (2000), 219(4), 514-25

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized ... [more ▼]

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized by this antibody still needs to be identified. We have characterized further its localization in vivo, its expression and subcellular localization in vitro, as well as its molecular nature. Histologic investigations of whole mouse embryos reveal an equally intense expression of RC2 immunostaining in radial glial cells in brain and spinal cord and in skeletal muscle. In glial cells cultures, the RC2 antibody recognizes an epitope located on the glial cytoskeleton and identified as an intermediate filament associated protein (IFAP) at the ultrastructural level. RC2 immunostaining in those cells is strongly dependent on the presence of a serum-derived activity. Serum-removal causes a decrease of the staining while adding serum back to the cells induces reexpression of RC2 immunoreactivity. By Western blotting, we find that in intermediate filament (IF) preparations obtained from cultured cerebellar glia, the RC2 antibody recognizes a 295-kDa protein whose expression is also dependent on the presence of serum in culture medium. In developing muscle cells, RC2 immunostaining is observed from the myoblast stage and disappears after complete myotube fusion. Both in vivo and in vitro, staining is first seen as a loose capping around myoblasts nuclei and progressively concentrates into Z-disks in association with the muscle IF protein desmin. The RC2 antibody also recognizes a 295-kDa protein band in muscle tissue protein extracts. Thus, the RC2 antibody recognizes a developmentally regulated cytoskeletal protein that is expressed, like other previously identified IFAPs, by cells of the glial and myogenic lineages and whose expression in vitro seems to be controlled by a signaling mechanism known to modulate astroglial morphology. [less ▲]

Detailed reference viewed: 80 (4 ULg)
Full Text
Peer Reviewed
See detailRegulation of Etioplast Pigment-Protein Complexes, Inner Membrane Architecture, and Protochlorophyllide a Chemical Heterogeneity by Light-Dependent Nadph:Protochlorophyllide Oxidoreductases a and B
Franck, Fabrice ULg; Sperling, U.; Frick, G. et al

in Plant Physiology (2000), 124(4), 1678-96

The etioplast of dark-grown angiosperms is characterized by the prolamellar body (PLB) inner membrane, the absence of chlorophyll, and the accumulation of divinyl and monovinyl derivatives of ... [more ▼]

The etioplast of dark-grown angiosperms is characterized by the prolamellar body (PLB) inner membrane, the absence of chlorophyll, and the accumulation of divinyl and monovinyl derivatives of protochlorophyll(ide) a [Pchl(ide) a]. Either of two structurally related, but differentially expressed light-dependent NADPH:Pchlide oxidoreductases (PORs), PORA and PORB, can assemble the PLB and form dark-stable ternary complexes containing enzymatically photoactive Pchlide-F655. Here we have examined in detail whether these polypeptides play redundant roles in etioplast differentiation by manipulating the total POR content and the PORA-to-PORB ratio of etiolated Arabidopsis seedlings using antisense and overexpression approaches. POR content correlates closely with PLB formation, the amounts, spectroscopic properties, and photoreduction kinetics of photoactive Pchlide, the ratio of photoactive Pchlide-F655 to non-photoactive Pchl(ide)-F632, and the ratio of divinyl- to monovinyl-Pchl(ide). This last result defines POR as the first endogenous protein factor demonstrated to influence the chemical heterogeneity of Pchl(ide) in angiosperms. It is intriguing that excitation energy transfer between different spectroscopic forms of Pchl(ide) in etiolated cotyledons remains largely independent of POR content. We therefore propose that the PLB contains a minimal structural unit with defined pigment stoichiometries, within which a small amount of non-photoactive Pchl(ide) transfers excitation energy to a large excess of photoactive Pchlide-F655. In addition, our data suggests that POR may bind not only stoichiometric amounts of photoactive Pchlide, but also substoichiometric amounts of non-photoactive Pchl(ide). We conclude that the typical characteristics of etioplasts are closely related to total POR content, but not obviously to the specific presence of PORA or PORB. [less ▲]

Detailed reference viewed: 17 (2 ULg)