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See detailHypofractionation in retinoblastoma: an increased risk of retinopathy.
Coucke, Philippe ULg; Schmid; Balmer et al

in Radiotherapy & Oncology (1993), 28(2), 157-161

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See detailNew Pyridobenzodiazepine Derivatives as Potential Antipsychotics: Synthesis and Neurochemical Study
Liégeois, Jean-François ULg; Bruhwyler, J.; Damas, Jacques ULg et al

in Journal of Medicinal Chemistry (1993), 36(15), 2107-14

The discovery of a new, safe, atypical antipsychotic remains an important challenge. To achieve this goal, a series of N-methylpiperazinopyrido[2,3-b] [1,4]- and -[1,5]- and -pyrido[4,3-b][1,4]- and -[1,5 ... [more ▼]

The discovery of a new, safe, atypical antipsychotic remains an important challenge. To achieve this goal, a series of N-methylpiperazinopyrido[2,3-b] [1,4]- and -[1,5]- and -pyrido[4,3-b][1,4]- and -[1,5]- benzodiazepines were synthesized. The dopaminergic (D1, D2), serotonergic (5-HT2), and cholinergic (M) affinities, frequently remarked in the action mechanisms of antipsychotic drugs, were determined using their respective in vitro receptor binding assays. All affinities were reduced for each compound. Optimal substituents were found to be in the 2- or 8-position for the retention of affinities, while substitution at the 5-position by acyl or alkyl groups dramatically diminished binding affinities. Pyridobenzodiazepine derivatives, such as clozapine, were found to be inactive or only weakly effective against apomorphine-mediated stereotypes in rats. In an original and complex behavioral model developed in dogs and successfully used to differentiate distinct classes of psychotropic drugs and to discriminate between typical and atypical neuroleptic drugs, 8-chloro-6-(4-methyl-1-piperazinyl)-11H-pyrido[2,3-b] [1,4]benzodiazepine (9), 8-methyl-6-(4-methyl-1-piperazinyl)-11H-pyrido [2,3-b][1,4]benzodiazepine (12), and 5-(4-methyl-1-piperazinyl)-11H-pyrido[2,3-b][1,5]benzodiazepine (16) showed most of the behavioral characteristics previously described for neuroleptics. Their neurochemical profiles, particularly their 5-HT2/D2 pKi ratios, were compatible with an atypical antipsychotic effect. These compounds were selected for further investigation. The proposed modulations could lead to new possibilities for the pharmacochemistry of diarylazepines. [less ▲]

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See detailThe Dual Role of Thymic Neurohypophysial-Related Self Peptides in T Cell Selection. Physiological and Pharmacological Implications
Geenen, Vincent ULg; Martens, Henri ULg; Robert, Françoise et al

in Annals of the New York Academy of Sciences (1993), 689

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See detailEffect of hot N(4S) atoms on the NO solar cycle variation in the lower thermosphere
Gérard, Jean-Claude ULg; Shematovich, V. I.; Bisikalo, D. V.

in Journal of Geophysical Research (1993), 98

The variation of the nitric oxide peak density near 110 km with solar activity is calculated using a photochemical diffusive model of thermospheric odd nitrogen. This model includes the reaction of ... [more ▼]

The variation of the nitric oxide peak density near 110 km with solar activity is calculated using a photochemical diffusive model of thermospheric odd nitrogen. This model includes the reaction of translationally excited ('hot') nitrogen atoms with O2 as a source of nitric oxide, in addition to the classical photochemistry. It is confirmed that the dissociation of N2 by energetic photoelectrons due to the ionization of atmospheric constituents by solar soft X-rays is an important source of atomic nitrogen which controls the observed NO maximum near 110 km. The consideration of the hot N(4S) source increases the NO peak density by 45 to 60 percent dependent on the solar activity level considered. The calculated NO peak density increases by a factor of about 3.5 from low to high solar activity conditions, in agreement with the Solar Mesosphere Explorer satellite observations. The absolute concentrations calculated in the model with an N(2D) effective yield of 54 percent from N2 electron impact dissociation are midway between the two sets of solar cycle NO variation measurements currently available. [less ▲]

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See detailCorrigenda to the 4th edition of the Bright Star Catalogue
Renson, Pierre ULg

in Bulletin d'Information du Centre de Données Stellaires (1993), 43

Not Available

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See detailUn messager intercellulaire original, le NO (monoxyde d'azote)
Liégeois, Jean-François ULg; Damas, Jacques ULg

in Revue Médicale de Liège (1993), 48(7), 406-13

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See detailVers l'interdiction d'expulsion des étrangers intégrés?, note sous Cour européenne des droits de l'homme, 26 mars 1992
Carlier, Jean-Yves ULg

in Revue Trimestrielle des Droits de l'Homme [= RTDH] (1993), 4(15), 449-466

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See detailDiagnostic des infections cutanées par les herpes virus de types simplex et varicelle-zona
Nikkels, Arjen ULg; Hermanns-Le, Trinh ULg; Tassoudji, Nazli ULg et al

in Revue Médicale de Liège (1993), 48(7), 401-405

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See detailCharacterization of the regulatory functions of varicella-zoster virus open reading frame-4 gene-product
Defechereux, Patricia; Melen-Lamalle, Laurence ULg; Baudoux, Laurence et al

in Journal of Virology (1993), 67(7), 4379-4385

Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ... [more ▼]

Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ICP27 of herpes simplex virus type 1. To investigate the regulatory properties of the ORF4 gene product, we performed a series of transient expression assays in Vero cells, using a plasmid expressing ORF4 as effector and several VZV genes and heterologous genes as targets. The VZV target plasmids contained promoter/regulatory regions from genes belonging to the three putative VZV kinetic classes fused to the chloramphenicol acetyltransferase (CAT) gene. The heterologous target plasmids consisted of promoter/regulatory regions of human cytomegalovirus, Rous sarcoma virus, and human immunodeficiency virus type 1 fused to the reporter gene. These experiments demonstrated that the ORF4 gene product activated expression of ORF62 in a dose-dependent fashion but had no effect on the expression of the three other putative immediate-early genes (ORF4, ORF61, and ORF63). When various amounts of ORF4 were transfected in the presence of early gene promoters, dose-dependent transactivation was evidenced with the thymidine kinase gene (ORF36) and the major DNA-binding protein gene (ORF29) promoters; interestingly, little activity was detected with the promoter of the DNA polymerase gene (ORF28). No activation of late gene expression, represented by the glycoprotein I and glycoprotein II genes, was seen even over a wide range of concentrations of input ORF4 plasmid. Expression of pCMVCAT, pRSVCAT, and pHIVCAT was also stimulated by the ORF4 gene product. CAT mRNA analysis showed that activation of VZV target promoters occurs at the transcriptional and/or posttranscriptional level. [less ▲]

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See detailPresurgical Octreotide Treatment in Acromegaly
Stevenaert, Achille ULg; Beckers, Albert ULg

in Acta Endocrinologica (1993), 129(1), 18-20

The effects of octreotide pretreatment before surgery were assessed in 48 acromegalic patients who underwent trans-sphenoidal surgery to remove pituitary adenoma, and compared with 107 patients who did ... [more ▼]

The effects of octreotide pretreatment before surgery were assessed in 48 acromegalic patients who underwent trans-sphenoidal surgery to remove pituitary adenoma, and compared with 107 patients who did not receive octreotide before surgery. Pretreated patients received octreotide 100 micrograms, thrice daily for 3 to 6 weeks (group 1), or 100 to 500 micrograms, thrice daily for 3 to 39 months (group 2). Total remission of symptoms was seen in 54%. Partial remission of symptoms occurred in 39%. Octreotide pretreatment reduced plasma GH levels by > or = 50% in all patients. GH levels were reduced to < 2 micrograms/1 in 3/14 group 1 cases and 13/34 group 2 cases. Tumour shrinkage occurred in 56.5% of patients. Marked tumour shrinkage (> 25%) occurred in more group 2 patients (10/32 vs 1/14). The tumour was soft in 39 patients, firm in 6 and hard in 3 cases. Soft consistency aided surgical removal of adenomas. Remission was greater in enclosed adenomas than in invasive tumours in both octreotide pretreated and nonpretreated acromegalics (76 vs 30%). Remission was more likely if the adenoma was less than 16 mm. Remission rate was significantly greater (p < 0.05) in all octreotide pretreated than in untreated patients for enclosed adenoma, but not for invasive adenoma. Octretide pretreatment can be recommended for three to four months prior to surgery. [less ▲]

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See detailLe clonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Francis ULg; Ectors, Fabien ULg; Delval, Alain et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1993), 148

In 1987, Prather et al. have performed the first embryo cloning by nuclear transfer in the bovine species. Since, many researchers try to develop and to apply the technique. While the enucleation of the ... [more ▼]

In 1987, Prather et al. have performed the first embryo cloning by nuclear transfer in the bovine species. Since, many researchers try to develop and to apply the technique. While the enucleation of the recipient oocyte, the injection of the donor blastomere and the fusion procedure are now well controlled, on the other hand, maturation and activation as the development and freezing of the cloned embryos need tobe more investigated. The cloned embryo is more fragile. An increase in embryonic mortality is observed after transfer in a recipient cow. [less ▲]

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See detailComparative evaluation of three heat and moisture exchangers during short-term postoperative mechanical ventilation
Sottiaux, Thierry; Mignolet, Ghislaine ULg; Damas, Pierre ULg et al

in CHEST (1993), 104(1), 220-224

This study compared performance of three heat and moisture exchangers (HME) during short-term postoperative mechanical ventilation. Temperature and absolute humidity (AH) were measured at various points ... [more ▼]

This study compared performance of three heat and moisture exchangers (HME) during short-term postoperative mechanical ventilation. Temperature and absolute humidity (AH) were measured at various points of the ventilatory circuit. There was no statistical difference between the groups, regarding ambient and body To, body weight, fraction of inspired oxygen, tidal volume, and respiratory rate. Only the hygroscopic HME (groups 2 and 3) provide adequate conditioning with regard to AH and To of the inspiratory gases. The performance of hydrophobic HME (group 1) was inferior and appears to be unsatisfactory. Indirect evaluation (variations of inspiratory gases and tracheal temperatures, AH of the expired gases) confirmed the superiority of the hygroscopic HME. These data suggest that humidification of inspiratory gases with a hygroscopic HME is a defensible practice during short-term postoperative mechanical ventilation. Performance of hydrophobic HME may be weak and can expose the patient to an unacceptable risk of endotracheal tube occlusion. [less ▲]

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See detailThird-body irradiation as an effective palliative treatment for painful multiple bone metastases resistant to chemo- or hormonal treatment
Douglas, P; Rossier, Ph.; Mirimanoff, R-O et al

in Radiotherapy & Oncology (1993), 28(1), 76-78

Fifty-three patients had 54 third-body areas irradiated for breast and prostate bone metastases using the third-body irradiation technique during a period of 6 years. These patients were previously ... [more ▼]

Fifty-three patients had 54 third-body areas irradiated for breast and prostate bone metastases using the third-body irradiation technique during a period of 6 years. These patients were previously treated with chemotherapy, hormonal therapy and limited field irradiation. Seventy percent responded completely and 24% partially. This modality is safe and effective for pain relief. [less ▲]

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See detailEffect of Transdermal 17 Beta-Estradiol and Oral Conjugated Equine Estrogens on Biochemical Parameters of Bone Resorption in Natural Menopause
Reginster, Jean-Yves ULg; Christiansen, C.; Dequinze, B. et al

in Calcified Tissue International (1993), 53

OBJECTIVE: To evaluate and compare the effects or oral and transdermal estrogen replacement therapy on biochemical markers of bone resorption in early postmenopausal women. DESIGN: Controlled, randomized ... [more ▼]

OBJECTIVE: To evaluate and compare the effects or oral and transdermal estrogen replacement therapy on biochemical markers of bone resorption in early postmenopausal women. DESIGN: Controlled, randomized group comparison. SETTING: Outpatient clinic for menopausal women and research into osteoporosis. SUBJECTS: Sixty healthy women menopausal for less than 5 years and who had never received any medications interfering with bone metabolism. INTERVENTIONS: The 60 women were randomly allocated to 3 months therapy with either oral conjugated estrogens (0.625 mg/day) (n = 28) or transdermal estradiol (50 micrograms/day) (n = 32) in cyclical combination with medroxyprogesterone acetate (5 mg/day). MAIN OUTCOME MEASURES: Traditional (urinary calcium/creatinine and hydroxyproline/creatinine) and the new specific (urinary pyridinoline/creatinine and deoxypyridinoline/creatinine) markers of bone resorption were determined before and after 3 months of treatment. RESULTS: In both groups, circulating levels of estrone and estradiol were significantly (P < 0.001) increased during treatment. In women treated with oral conjugated equine estrogens, urinary calcium/creatinine and hydroxyproline/creatinine ratios were significantly (P < 0.05) reduced. Pyridinoline/creatinine ratio fell from 69.1 (4) [mean (SEM)] to 50 (4) mumol/mumol (P < 0.01) and deoxypyridinoline/creatinine ratio fell from 10.8 (1) [mean (SEM)] to 8.3 (0.8) mumol/mumol (P < 0.01). In the group treated with transdermal estradiol, urinary hydroxyproline/creatinine ratio was significantly (P < 0.05) reduced. Pyridinoline/creatinine ratio fell from 66.3 (4) [mean (SEM)] to 46.2 (3) mumol/mumol (P < 0.01) and deoxypyridinoline/creatinine ratio fell from 11.5 (1.5) [mean (SEM)] to 7.7 (0.6) mumol/mumol (P < 0.01). There were no differences between the evolution of the biochemical variables in the two groups. CONCLUSION: These results suggest that oral conjugated equine estrogens and transdermal estradiol, in the given doses, are equally effective in reducing postmenopausal bone resorption. [less ▲]

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See detailRecent and prospective decorrelation techniques for image processing
Comes, Serge; Maes, Stéphane; Van Droogenbroeck, Marc ULg

in Annales des Télécommunications = Annals of Telecommunications (1993), 48(7-8), 390-403

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See detailInteractions between Tumoral Mcf7 Cells and Fibroblasts on Matrigel and Purified Laminin
Noël, Agnès ULg; Nusgens, Betty ULg; Lapiere, C. H. et al

in Matrix (Stuttgart, Germany) (1993), 13(4), 267-73

A reconstituted basement membrane (matrigel) and/or fibroblasts promote the growth of human breast tumors in athymic nude mice. We have investigated in vitro the effect of matrigel or purified ... [more ▼]

A reconstituted basement membrane (matrigel) and/or fibroblasts promote the growth of human breast tumors in athymic nude mice. We have investigated in vitro the effect of matrigel or purified glycoproteins (laminin and fibronectin) on tumoral MCF7 cells-fibroblasts interactions. In coculture on matrigel, MCF7 cells organized into clusters attached on top of fibroblasts aggregates. During the process resulting in tumor cells-fibroblasts aggregation, fibroblasts actively migrated while MCF7 cells were passively transported. Using purified proteins, specific antibodies and synthetic peptides, we show that cell aggregation induced by immobilized and soluble laminin is antagonized by exogenous fibronectin or fibronectin synthesized by fibroblasts. [less ▲]

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See detailSuprasellare Raumforderung als Erstmanifestation einer Tuberkulose im Kindesalter.
Reul, J.; RAMAEKERS, Vincent ULg; Thron, A.

in Deutsche medizinische Wochenschrift (1946) (1993), 118(22), 820-4

Three days after having sustained a mild trauma to the head a seven-year-old boy developed seizure-like rotatory vertigo. Computed tomography, magnetic resonance imaging and cerebral angiography ... [more ▼]

Three days after having sustained a mild trauma to the head a seven-year-old boy developed seizure-like rotatory vertigo. Computed tomography, magnetic resonance imaging and cerebral angiography demonstrated a suprasellar space-occupying lesion. Pulmonary tuberculosis was discovered in subsequent diagnostic work-up. Cerebrospinal fluid examination was unremarkable except for immunological tests (tuberculostearic acid) which pointed to central nervous system (CNS) involvement so that a tuberculoma was suspected. The lesion decreased in size on tuberculostatic treatment (200 mg/d isoniazid, 200 mg/d rifampicin, two times 250 mg/d pyrazinamide). In the next 12 months there merely persisted a mild abnormality of the blood-brain barrier with a little contrast-medium uptake, which regressed in the following 6 months. The differential diagnosis between CNS tuberculosis and brain tumour or pyogenic abscess can be difficult in children if there are no pulmonary signs and/or the cerebrospinal fluid is normal. [less ▲]

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See detailThe Addition of Nine Residues at the C-Terminus of Human Prolactin Drastically Alters Its Biological Properties
Goffin, Vincent; Struman, Ingrid ULg; Goormaghtigh, E. et al

in European Journal of Biochemistry (1993), 214(2), 483-90

We have added nine extra residues to the C-terminal of human prolactin and analysed the effect of this mutation on the ability of the hormone to bind to its lactogenic receptor and to induce Nb2 cell ... [more ▼]

We have added nine extra residues to the C-terminal of human prolactin and analysed the effect of this mutation on the ability of the hormone to bind to its lactogenic receptor and to induce Nb2 cell division. Both properties are markedly affected when compared to the natural 23-kDa human prolactin. Since no alteration of the global protein folding was detected either by circular dichroism or by infrared spectroscopy, the decrease in biological potency can be exclusively attributed to an effect of the nine additional residues on their near environment. From infrared analysis and secondary structure prediction, the elongated tail is assumed to be involved in a beta-sheet with a few residues initially belonging to the fourth helix. Moreover, from the X-ray structures of porcine and human growth hormones, two proteins homologous to prolactins, the nine extra residues are likely to fold within a concave pocket delimited by helices 1 and 4, and the second half of the loop connecting helices 1 and 2 (loop 1). Thereby, we suggest that the additional residues prevent some residues belonging to this pocket from interacting with the lactogenic receptor. This is in perfect agreement with our earlier proposal that the binding site of prolactin to the lactogenic receptor is homologous to that of growth hormone to the somatogenic receptor, i.e. essentially composed of residues belonging to this concave pocket. [less ▲]

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See detailCoupled buoyancy and thermocapillary convection in a viscoelastic Maxwell fluid
Dauby, Pierre ULg; Parmentier, P.; Lebon, Georgy ULg et al

in Journal of Physics : Condensed Matter (1993), 5

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See detailCharacterization of the Sporulation-Related Gamma-D-Glutamyl-(L)Meso-Diaminopimelic-Acid-Hydrolysing Peptidase I of Bacillus Sphaericus NCTC 9602 as a Member of the Metallo(Zinc) Carboxypeptidase A Family. Modular Design of the Protein
Hourdou, Marie-Laure; Guinand, Micheline; Vacheron, Marie-Jeanne et al

in Biochemical Journal (1993), 292(Pt 2), 563-570

The sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 has been analysed by proton-induced X-ray emission. It contains 1 equivalent ... [more ▼]

The sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 has been analysed by proton-induced X-ray emission. It contains 1 equivalent Zn2+ per mol of protein. As derived from gene cloning and sequencing, the B. sphaericus Zn peptidase I is a two-module protein. A 100-amino-acid-residue N-terminal domain consisting of two tandem segments of similar sequences, is fused to a 296-amino-acid-residue C-terminal catalytic domain. The catalytic domain belongs to the Zn carboxypeptidase A family, the closest match being observed with the Streptomyces griseus carboxypeptidase [Narahashi (1990) J. Biochem. 107, 879-886] and with the family prototype, bovine carboxypeptidase A. The catalytic domain of the B. sphaericus peptidase I possesses, distributed along the amino-acid sequence, peptide segments, a triad His162-Glu165-His307 and a dyad Tyr347-Glu366 that are equivalent to secondary structures, the zinc-binding triad His69-Glu72-His196 and the catalytic dyad Tyr248-Glu270 of bovine carboxypeptidase A respectively. The N-terminal repeats of the B. sphaericus peptidase I have similarity with the C-terminal repeats of the Enterococcus hirae muramidase 2, the Streptococcus (now Enterococcus) faecalis autolysin and the Bacillus phi PZA and phi 29 lysozymes, to which a role in the recognition of a particular moiety of the bacterial cell envelope has been tentatively assigned. Detergents enhance considerably the specific activity of the B. sphaericus peptidase I. [less ▲]

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See detailClinical application of a noninvasive multi-electrode array EMG for the recording of single motor unit activity.
RAMAEKERS, Vincent ULg; Disselhorst-Klug, C.; Schneider, J. et al

in Neuropediatrics (1993), 24(3), 134-8

Using a recently developed noninvasive EMG recording technique with multi-electrode arrays we investigated the pattern and distribution of motor unit action potentials (MUAP) following maximal voluntary ... [more ▼]

Using a recently developed noninvasive EMG recording technique with multi-electrode arrays we investigated the pattern and distribution of motor unit action potentials (MUAP) following maximal voluntary contraction of the musculus abductor pollicis brevis. An additional parameter, i.e. muscular conduction velocity (CV) in single motor units, was calculated from the multi-electrode array EMG recordings. From 63 healthy children of various age the normal EMG pattern and CV were derived and compared to the EMG of diagnosed patients known to suffer from Duchenne muscular dystrophy and from spinal muscular atrophy. In normal individuals the muscular CV in neonates was lowest at 1-2 m/s and gradually reached a plateau of 2.9-4 m/s from the age of 4 years onwards. The EMG in 31 children with Duchenne muscular dystrophy showed an abnormal pattern with low amplitude action potentials. In 30 out of 31 patients a significantly lower muscular CV was found. In 10 children with spinal muscular atrophy the EMG showed action potentials of abnormally large amplitude and a reduced recruitment of firing motor units. The muscular CV remained within the normal range. Compared to classical needle EMG the application of this new noninvasive EMG technique in children is painless and offers an easy-to-handle diagnostic tool to differentiate between neuromuscular diseases of denervating or of myopathic origin. [less ▲]

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See detailLaryngeal Mask
Janssens, Marc ULg; Lamy, Maurice ULg

in Intensive Care World (1993), 10(2), 99-102

The laryngeal mask, provides a totally patent airway when positioned in the hypopharynx with remarkable patient tolerance, even at very light planes of sedation. The major advantages of the laryngeal mask ... [more ▼]

The laryngeal mask, provides a totally patent airway when positioned in the hypopharynx with remarkable patient tolerance, even at very light planes of sedation. The major advantages of the laryngeal mask are its ease of insertion, the absence of contact with the vocal cards, and the fact that it frees the hands of the anesthesiologist. Contraindications to its use result from its failure to seal the airway against regurgitation of gastric content. Laryngeal masks can be used easily instead of facial masks during anesthesia with spontaneous ventilation and, with experience, can be used for longer procedures using controlled ventilation. Suspected difficult intubation and establishment of a patent airway in emergency conditions are good indications for the use of this device. The laryngeal mask does not replace endotracheal intubation. It can, however, permit better management of the airway while waiting for personnel trained in endotracheal intubation. The nature of the pulmonary pathology seen in intensive care patients limits use of the laryngeal mask during intensive care. In the operating room few complications have been described, and postoperative discomfort is minimal. The laryngeal mask is a device positioned in the hypopharynx which allows separation of the digestive tract from the airway, without violation of either the larynx or the upper oesophageal sphincter. An endotracheal tube, because of its positioning, hinders normal glottic movement and narrows the airway.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailGravitational lensing statistics based on a large sample of highly luminous quasars
Surdej, Jean ULg; Claeskens, Jean-François ULg; Crampton, D. et al

in Astronomical Journal (The) (1993), 105

Results on gravitational lensing statistics applied to a sample of 469 highly luminous quasars are reported. The objects were directly imaged, either from the ground under optimal seeing conditions, or ... [more ▼]

Results on gravitational lensing statistics applied to a sample of 469 highly luminous quasars are reported. The objects were directly imaged, either from the ground under optimal seeing conditions, or using the HST. We have derived values for the effectiveness parameter F of galaxies, modeled by means of singular isothermal spheres, to produce macrolensed images of distant quasars, and upper limits on the density parameter Omega(L) of compact objects with masses between 10 exp 10 and 10 exp 12 solar masses. Adopting H(0)= 50 km/s/Mpc, Omega(0)= 1, and Lambda = 0, we find that at the 99.7 percent confidence level, F is between 0.005 and 0.478 and that Omega(L) is less than 0.02. A critical discussion of these results is presented. Finally, comparing the efficiencies of ground-based and space instruments used to search for gravitational lens systems among highly luminous quasars, we conclude that for the near future, ground-based direct imaging characterized by a good dynamical range still constitutes the best observational strategy. [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 11. Synthesis and use of alkylaluminium dialkoxides and dithiolates as promoters of hydroxy telechelic poly(epsilon-capprolactone) and alph,omega-dihydroxy triblock copolymers containing outer polyester blocks
Dubois, Philippe; Degée, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1993), 26(11), 2730-2735

Diethylaluminum dialkoxides (Et2AlOXOAlEt2, where X is either an aliphatic radical or a polymer chain) and related dithiolates have been investigated as potential initiators for the ring-opening ... [more ▼]

Diethylaluminum dialkoxides (Et2AlOXOAlEt2, where X is either an aliphatic radical or a polymer chain) and related dithiolates have been investigated as potential initiators for the ring-opening polymerization of ε-caprolactone. Hydroxy telechelic poly(ε-caprolactone) and alpha,omega-dihydroxypoly(ε-caprolactone-b-X-b-ε-caprolactone) have been accordingly prepared and characterized. A hydroxy group is selectively attached at both ends as demonstrated by IR and H-1 NMR spectroscopies and confirmed by titration of the derivatized alpha,omega-dicarboxylic acid (co)polymers. Polymerization of epsilon-caprolactone is living and yields linear polyester chains of a predictable molecular weight and of quite a narrow molecular weight distribution. [less ▲]

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See detailHalofantrine resistance in African countries
Carme, Bernard; Gay, Frédéric; Hayette, Marie-Pierre ULg et al

in Lancet (1993), 341

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See detailWich is the Star V380 Per?
Renson, Pierre ULg

in Information Bulletin on Variable Stars (1993), 3887

Not Available

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See detailErratum - Q:1208+1011 - the Most Distant Multiply Imaged Quasar or a Binary
Magain, Pierre ULg; Surdej, Jean ULg; Vanderriest, C. et al

in Astronomy and Astrophysics (1993), 272

In the title of this Letter, the word "multiply" was accidentally omitted. The correct title is shown above.

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See detailIntracellular cation concentrations in essential hypertension and chronic renal failure
Krzesinski, Jean-Marie ULg; Du, F.; Rorive, Georges ULg

in Clinical & Experimental Hypertension (1993), 15(3), 461-478

The aim of this study was to test basal and after treatment erythrocyte sodium and calcium concentrations, and calcium-ATPase activity and platelet cytosolic free calcium and pH in 20 normotensive ... [more ▼]

The aim of this study was to test basal and after treatment erythrocyte sodium and calcium concentrations, and calcium-ATPase activity and platelet cytosolic free calcium and pH in 20 normotensive controls, 20 hemodialysis-dependent chronic renal failure patients and in 18 essential hypertensives. Prior to treatment, essential hypertensive and uremic patients presented similar higher platelet calcium concentrations and lower pH than the normotensive control group. The erythrocyte sodium, calcium, and magnesium concentrations were only significantly elevated in chronic renal failure, with a significant decrease in the calcium-ATPase activity in the latter population. Hemodialysis partially reversed these intracellular ionic abnormalities with normalization of platelet pH. Significant correlations have been noted between weight loss and decreases in platelet calcium concentration (r = 0.60, p < 0.01) or in erythrocyte sodium (r = 0.50, p < 0.05). The systolic blood pressure decrease was only correlated to the increase in calcium-ATPase activity (r = 0.57, p < 0.05). Antihypertensive treatment (captopril and nifedipine) only tended to normalize the intracellular calcium concentration with correlation between the decrease of the latter and blood pressure decrease (r = 0.64 for the systolic blood pressure and 0.68 for the diastolic blood pressure, p < 0.01). Thus, in essential hypertension and in uremia, some cellular ionic abnormalities exist in platelets in baseline condition. Moreover, in uremia, erythrocyte presents abnormal ionic pattern. Some, but not all of these abnormalities could be corrected by treatment affecting blood pressure (cellular calcium) in essential hypertension or by hemodialysis (cellular sodium, calcium, and pH). In the latter treatment, the changes are linked to extracellular fluid modification. In essential hypertension, the intracellular calcium reduction was linked to blood pressure decrease. [less ▲]

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See detailLes manifestations cutanées liées à l'insuffisance veineuse chronique IVC
Dezfoulian, Bita ULg; de LA BRASSINNE, Michel ULg

in Medical Trends (1993), 5

Toutes les altérations consécutives à la dilatation des veines des membres inférieurs, à l'incompétence de leurs valvules et à l'augmentation de pression veineuse constituent l'insuffisance veineuse ... [more ▼]

Toutes les altérations consécutives à la dilatation des veines des membres inférieurs, à l'incompétence de leurs valvules et à l'augmentation de pression veineuse constituent l'insuffisance veineuse chronique (IVC). Après avoir brièvement rappelé les mécanismes physiopathologiques à la base de l'IVC, nous évoquons dans cet article les manifestations cutanées associées à cette dernières ainsi que les traitements possibles, lorsqu'ils existent. [less ▲]

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See detailCloning and Sequencing of the Low-Affinity Penicillin-Binding Protein 3r-Encoding Gene of Enterococcus Hirae S185: Modular Design and Structural Organization of the Protein
Piras, Graziella; Raze, Dominique; el Kharroubi, Aboubaker et al

in Journal of Bacteriology (1993), 175(10), 2844-2852

The clinical isolate Enterococcus hirae S185 has a peculiar mode of resistance to penicillin in that it possesses two low-affinity penicillin-binding proteins (PBPs): the 71-kDa PBP5, also found in other ... [more ▼]

The clinical isolate Enterococcus hirae S185 has a peculiar mode of resistance to penicillin in that it possesses two low-affinity penicillin-binding proteins (PBPs): the 71-kDa PBP5, also found in other enterococci, and the 77-kDa PBP3r. The two PBPs have the same low affinity for the drug and are immunochemically related to each other. The PBP3r-encoding gene has been cloned and sequenced, and the derived amino acid sequence has been compared by computer-assisted hydrophobic cluster analysis with that of the low-affinity PBP5 of E. hirae R40, the low-affinity PBP2' of Staphylococcus aureus, and the PBP2 of Escherichia coli used as the standard of reference of the high-M(r) PBPs of class B. On the basis of the shapes, sizes, and distributions of the hydrophobic and nonhydrophobic clusters along the sequences and the linear amino acid alignments derived from this analysis, the dyad PBP3r-PBP5 has an identity index of 78.5%, the triad PBP3r-PBP5-PBP2' has an identity index of 29%, and the tetrad PBP3r-PBP5-PBP2'-PBP2 (of E. coli) has an identity index of 13%. In spite of this divergence, the low-affinity PBPs are of identical modular design and possess the nine amino acid groupings (boxes) typical of the N-terminal and C-terminal domains of the high-M(r) PBPs of class B. At variance with the latter PBPs, however, the low-affinity PBPs have an additional approximately 110-amino-acid polypeptide stretch that is inserted between the amino end of the N-terminal domain and the carboxy end of the membrane anchor. While the enterococcal PBP5 gene is chromosome borne, the PBP3r gene appears to be physically linked to the erm gene, which confers resistance to erythromycin and is known to be plasmid borne in almost all the Streptococcus spp. examined. [less ▲]

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See detailInduction of a Serotonin-S2 Receptor Blockade During Early or Late Stage of Acute Respiratory Distress Syndrome in Double-Muscled Calves: A Comparative Study
Genicot, Bruno; Mouligneau, Frédéric; Lindsey, J. K. et al

in Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine (1993), 40(4), 241-248

Ninety-four Belgian White and Blue double-muscled calves were involved in this study which aimed to compare the efficacy of a serotonin-S2 receptor blockade at two different stages of an acute respiratory ... [more ▼]

Ninety-four Belgian White and Blue double-muscled calves were involved in this study which aimed to compare the efficacy of a serotonin-S2 receptor blockade at two different stages of an acute respiratory distress syndrome (ARDS), i.e. at the occurrence of first clinical signs or when another anti-inflammatory compound was clinically shown to be ineffective. Metrenperone, a 5-hydroxytryptamine (5-HT2) blocker, was injected (intramuscularly, 5 times, at 12 hourly intervals, dose rate: 0.1 mg/kg) to (1) 58 calves referred to our laboratory after a treatment (group I) which lasted from 3 to 5 days and which did not improve the clinical status of the animals and (2) 36 calves investigated as soon as first clinical signs occurred (group II). Following the severity of the ARDS, the animals from group I needed to be classified into 2 groups: group IA (moderate ARDS - n = 45) and group IB (severe ARDS - n = 13). For animals in group I, the antibacterial compound previously used was maintained and administered during 4 more days whilst the previous anti-inflammatory drug was suppressed and replaced by metrenperone administered as mentioned above. In group II, each animal was treated with ceftiofur sodium as an antibacterial agent. A clinical score and a breathing score were calculated at each step of the investigation period, i.e. before (T0) and 1 hour (T1) after the first treatment, during the interval 12H-48H (T2) which followed this treatment and 168 H (T3) after this treatment. Four clinical parameters were also taken into account separately: rectal and cutaneous temperatures, respiratory and heart rates.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailRetinoic Acid Stimulates Regeneration of Mammalian Auditory Hair Cells
Lefèbvre, Philippe ULg; Malgrange, Brigitte ULg; Staecker, H. et al

in Science (1993), 260(5108), 692-5

Sensorineural hearing loss resulting from the loss of auditory hair cells is thought to be irreversible in mammals. This study provides evidence that retinoic acid can stimulate the regeneration in vitro ... [more ▼]

Sensorineural hearing loss resulting from the loss of auditory hair cells is thought to be irreversible in mammals. This study provides evidence that retinoic acid can stimulate the regeneration in vitro of mammalian auditory hair cells in ototoxic-poisoned organ of Corti explants in the rat. In contrast, treatment with retinoic acid does not stimulate the formation of extra hair cells in control cultures of Corti's organ. Retinoic acid-stimulated hair cell regeneration can be blocked by cytosine arabinoside, which suggests that a period of mitosis is required for the regeneration of auditory hair cells in this system. These results provide hope for a recovery of hearing function in mammals after auditory hair cell damage. [less ▲]

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See detailThe Mechanism of Action of DD-Peptidases: The Role of Tyrosine-159 in the Streptomyces R61 DD-Peptidase
Wilkin, Jean-Marc; Jamin, Marc; Damblon, Christian ULg et al

in Biochemical Journal (1993), 291(Part 2), 537-544

Tyrosine-159 of the Streptomyces R61 penicillin-sensitive DD-peptidase was replaced by serine or phenylalanine. The second mutation yielded a very poorly active protein whose rate of penicillin binding ... [more ▼]

Tyrosine-159 of the Streptomyces R61 penicillin-sensitive DD-peptidase was replaced by serine or phenylalanine. The second mutation yielded a very poorly active protein whose rate of penicillin binding was also drastically decreased, except for the reactions with nitrocefin and methicillin. The consequences of the first mutation were more surprising, since a large proportion of the thiolesterase activity was retained, together with the penicillin-binding capacity. Conversely, the peptidase properties was severely affected. In both cases, a drastic decrease in the transferase activity was observed. The results are compared with those obtained by mutation of the corresponding residue in the class A beta-lactamase of Streptomyces albus G. [less ▲]

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See detailHeterogeneity versus variability in Neuhauser megalocornea-mental retardation (MMR) syndromes: Report of new cases and delineation of 4 probable types
Verloes, Alain ULg; Journel, H.; Elmer, C. et al

in American Journal of Medical Genetics (1993), 46(2), 132-137

Megalocornea (corneal diameter > or = 13 mm) is associated with mental and neurological impairment, and minor anomalies in Neuhauser syndrome (megalocornea-mental retardation syndrome). Here we report 4 ... [more ▼]

Megalocornea (corneal diameter > or = 13 mm) is associated with mental and neurological impairment, and minor anomalies in Neuhauser syndrome (megalocornea-mental retardation syndrome). Here we report 4 new cases of megalocornea and mental retardation. Those unrelated patients have a consistent pattern of anomalies with possible recessive inheritance which clearly differs from that of the original patients of Neuhauser et al. [1975]. We discuss the heterogeneity of the syndromes with megalocornea and mental retardation. Based on these cases and on a review of the literature, we suggest a provisional clinically oriented classification in 5 subtypes: (1) a recessive form type Neuhauser (with iris hypoplasia and minor anomalies), (2) a recessive form type Frank-Temtamy (with camptodactyly, scoliosis and growth retardation), (3) a recessive type 3, including our 4 personal cases (with normal irides, severe hypotonia, relative or absolute macrocephaly and minor anomalies), (4) a possible Frydman type (with normal irides, megalencephaly and obesity), and (5) provisionally unclassifiable cases. [less ▲]

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See detailLes étoiles doubles ou multiples et leurs magnitudes.
Renson, Pierre ULg

in Ciel (Le) (1993), 55

Not Available

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See detailThird supplement to the catalogue of observed periods of Ap stars
Catalano, F. A.; Renson, Pierre ULg; Leone, F.

in Astronomy and Astrophysics. Supplement Series (1993), 98

New data on the periods of Ap stars with references are presented. Twelve further stars are introduced for which a periodic variability has recently been discovered or not reported in previous issues of ... [more ▼]

New data on the periods of Ap stars with references are presented. Twelve further stars are introduced for which a periodic variability has recently been discovered or not reported in previous issues of this catalog. For many stars also present in previous issues of the catalog new determinations of the periods are given. Recently attributed variable star names are also quoted. [less ▲]

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See detailOn the reduction of narrow-band photometry
Manfroid, Jean ULg

in Astronomy and Astrophysics (1993), 271

A method for the reduction of narrow-band photometry is presented based on a multi-night, multi-star, multi-telescope model, and incorporating the spectral properties of the atmosphere.

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See detailA New, Highly Sensitive Method for the Detection and Quantification of Penicillin-Binding Proteins
Galleni, Moreno ULg; Lakaye, Bernard ULg; Lepage, Sophie et al

in Biochemical Journal (1993), 291((Pt 1)), 19-21

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye ... [more ▼]

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells. [less ▲]

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See detailEfficiency of 5-Hydroxytryptamine Receptor Blockade as Therapeutic Measure During Acute Respiratory Distress Syndrome in Double-Muscled Cattle
Genicot, Bruno; Mouligneau, Frédéric; Lindsey, J. K. et al

in Zentralblatt für Veterinarmedizin. Reihe A (1993), 40(3), 185-193

During this investigation, which involved 58 Belgian White and Blue double-muscled calves affected by a naturally occurring Acute Respiratory Distress Syndrome, the clinical efficiency of a 5-HT2 receptor ... [more ▼]

During this investigation, which involved 58 Belgian White and Blue double-muscled calves affected by a naturally occurring Acute Respiratory Distress Syndrome, the clinical efficiency of a 5-HT2 receptor blockade with metrenperone (group A) was compared to the efficiency of a non-steroidal (flunixine meglumine--group B) and a steroidal (prednisolone sodium succinate--group C) antiinflammatory drug. Each animal of this trial was treated with ceftiofur sodium as antimicrobial agent. A clinical score and a breathing score were calculated at each step of the investigation period, i.e. before (T0) and 1 hour (T1), 12 hours (H), 24 H, 48H and 168 H (T3) after the first treatment, the interval 12H-48H being considered as period T2. Three clinical parameters were also taken into account separately: rectal and peripheral temperatures and heart rate. A significant improvement of the clinical score was registered at T2 in group A and at T3 in groups A and B, while this score did not significantly change in group C. In group A, the breathing score was significantly improved at T2 and T3, but not in groups B and C. Peripheral and rectal temperatures recorded at T1 were, in group A, significantly increased and decreased respectively, but not significantly changed in groups B and C. The proportions requiring change of treatment during the investigation period were significantly (P = 0.022) different in the three groups, being 5.6, 21.4 and 50.0% in groups A, B and C respectively.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailAngiosarcome hépatique chez une patiente ayant présenté une hépatite autoimmune
Reginster, M. A.; Mazy, V.; Waltregny, David ULg et al

in Revue Médicale de Liège (1993), 48(4), 220-3

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See detailRecovery from neurological deficits following biotin treatment in a biotinidase Km variant
RAMAEKERS, Vincent ULg; Brab, M.; Rau, G. et al

in Neuropediatrics (1993), 24(2), 98-102

A 15-year-old boy suffered from progressive bilateral optic neuropathy of acute onset at the age of 10 years. Subsequently he developed spastic paraparesis and a predominantly motor type neuro-axonal ... [more ▼]

A 15-year-old boy suffered from progressive bilateral optic neuropathy of acute onset at the age of 10 years. Subsequently he developed spastic paraparesis and a predominantly motor type neuro-axonal neuropathy in all limbs. The basic error has been elucidated to be due to an unusual biotinidase Km variant with biphasic enzyme kinetics causing systemic biotin depletion and consequent multiple biotin-dependent carboxylase deficiency. After daily oral substitution with 10 mg biotin metabolic derangements subsided rapidly. Follow-up studies over one year after substitution with biotin demonstrated remarkable recovery from part of the previously present neuro-ophthalmological, motor and cognitive deficits. The previously extinguished flash-evoked visual potentials now showed clear responses after six months of substitution with biotin. In contrast with reports in literature, these findings indicated that neurological damage associated with biotinidase deficiency, rather than being permanent, is to some extent reversible. [less ▲]

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See detailGrowth-induced haemodynamic changes in healthy Friesian calves
Amory, Hélène ULg; Desmecht, Daniel ULg; Linden, Annick ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1993), 132(17), 426-434

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See detailAdaptive Mathematical Morphology for Range Imagery
Verly, Jacques ULg; Delanoy, Richard L.

in IEEE Transactions on Image Processing (1993), 2(2), 272-275

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See detailSpontaneous Rupture of the Iliac Vein
Van Damme, Hendrik ULg; Hartstein, Gary ULg; Limet, Raymond ULg

in Journal of Vascular Surgery (1993), 17(4), 757-8

A spontaneous rupture of the left iliac vein is described. A 2 cm tear on the anterior surface of the left iliac vein was discovered at emergency laparotomy on a patient who was suspected of having a huge ... [more ▼]

A spontaneous rupture of the left iliac vein is described. A 2 cm tear on the anterior surface of the left iliac vein was discovered at emergency laparotomy on a patient who was suspected of having a huge intraabdominal hemorrhage. Prodromal symptoms, cause, and treatment are discussed. The nine previously reported cases are reviewed. This case represents the seventh successful repair of an idiopathic rupture of an iliac vein. [less ▲]

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See detailCongenital cervical spinal muscular atrophy: a non-familial, non progressive condition of the upper limbs.
Hageman, G.; RAMAEKERS, Vincent ULg; Hilhorst, B. G. et al

in Journal of neurology, neurosurgery, and psychiatry (1993), 56(4), 365-8

Two patients with congenital cervical spinal muscular atrophy had symmetrical severe muscle weakness and wasting confined to the upper limbs, areflexia and congenital contractures. The shoulders were ... [more ▼]

Two patients with congenital cervical spinal muscular atrophy had symmetrical severe muscle weakness and wasting confined to the upper limbs, areflexia and congenital contractures. The shoulders were internally rotated, elbows extended and wrists flexed. There were no sensory or bulbar symptoms, scoliosis, long tract signs or lower limb involvement. This condition should be regarded as a neurogenic type of arthrogryposis, limited to the upper limbs. [less ▲]

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See detailDiscrimination of animals treated with growth promoters
Scippo, Marie-Louise ULg; Degand, Guy ULg; Gaspar, Pol et al

in Meat Focus International (1993)

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See detailL'information financière, fondement de la gestion stratégique des Petites et Moyennes Entreprises familiales
Van Caillie, Didier ULg

in Comptabilité et Fiscalité Pratiques (1993), 93/4

This paper highlights the role of financial information as a key element which is underlying to any strategic management policy in family businesses

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See detailL'age: un facteur limitant pour la chirurgie carotidienne?
Van Damme, Hendrik ULg; Declercq, I.; Smitz, Simon ULg et al

in Revue Médicale de Liège (1993), 48(4), 195-206

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See detailExpression of the C-Erbb2 Gene in the Bt474 Human Mammary Tumor Cell Line: Measurement of C-Erbb2 Mrna Half-Life
Pasleau, Françoise ULg; Grooteclaes, Madeleine; Gol-Winkler, Rose

in Oncogene (1993), 8(4), 849-54

BT474 and SK-BR-3 mammary adenocarcinoma cells contain eight copies of the c-erbB2 gene but overexpress the mRNA 80 times over the levels measured in normal breast or in the HBL-100 cell line. Using ... [more ▼]

BT474 and SK-BR-3 mammary adenocarcinoma cells contain eight copies of the c-erbB2 gene but overexpress the mRNA 80 times over the levels measured in normal breast or in the HBL-100 cell line. Using Northern blot analysis and molecular titration based on RNAase protection assay, the decrease in the c-erbB2 mRNA level was monitored in BT474 cells treated with actinomycin D from 1 up to 24 h. The c-erbB2 degradation rate during the first 12 h corresponds to a calculated c-erbB2 mRNA half-life of approximately 7 h. Forty percent of the mRNA present in the cells before treatment remains undegraded after transcription has been blocked for 24 h. Pretreatment with cycloheximide results in complete mRNA degradation in 24 h, suggesting that labile proteins stabilize part of the c-erbB2 mRNA population. Comparison with the c-erbB2 mRNA turnover in HBL-100 'normal' cells indicated that the accumulation of the c-erbB2 gene product in the tumor cells is not the result of stabilization of the messenger. Rather, it is correlated with an increased rate of c-erbB2 mRNA transcription as indicated by run-on transcription assays. Both BT474 and SK-BR-3 tumor cell lines were found to synthesize 20-40 times more c-erbB2 mRNA than HBL-100 cells. [less ▲]

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See detailL'adolescent face au choix des études supérieures
Stassart, Martine ULg

in Cahiers du CEP (Centre d'Etudes Pathoanalytiques) (1993), 1

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See detailL'hyperréactivité bronchique non spécifique: données épidémiologiques et signification clinique
Louis, Renaud ULg; Corhay, Jean-Louis ULg; Bury, Thierry ULg et al

in Revue Médicale de Liège (1993), 48(4), 213-9

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See detailExploration et traitement des photodermatoses
Nikkels, Arjen ULg; Mallet, V.; Pierard, Gérald ULg

in Revue Médicale de Liège (1993), 48(4), 189-194

Le diagnostic d'une photodermatose est facilement évoqué devant une éruption rythmée par l'exposition au soleil. L'exploration photobiologique est pourtant nécessaire pour le classement nosologique de ces ... [more ▼]

Le diagnostic d'une photodermatose est facilement évoqué devant une éruption rythmée par l'exposition au soleil. L'exploration photobiologique est pourtant nécessaire pour le classement nosologique de ces affections. Nous présentons une revue de l'exploration photobiologique, de ses indications et interprétation, ainsi que des mesures thérapeutiques qui peuvent en découler. [less ▲]

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See detailAnalysis of Endotoxin Effects on Pulmonary Circulation in Terms of Pressure-Flow Characteristics
D'Orio, Vincenzo ULg; Fatemi, M.; Marnette, J. M. et al

in Circulatory Shock (1993), 39(4), 285-92

The purpose of the present work was to explore the hypothesis that pulmonary vasoconstriction secondary to endotoxin insult results mainly from an increase in the critical closing pressure of the ... [more ▼]

The purpose of the present work was to explore the hypothesis that pulmonary vasoconstriction secondary to endotoxin insult results mainly from an increase in the critical closing pressure of the pulmonary vessels. Specifically, we reasoned that in the face of a Starling resistor located between pulmonary arteries and left atrium, upstream transmission of increased left atrial pressure (Pla) would be inversely related to the level of the pressure intercept (Pi) obtained by extrapolation from the linear pulmonary arterial pressure (Ppa)--flow (Q degrees) plot. Six dogs (group E) were infused with Escherichia coli endotoxin (0.25 microgram/kg/min) for 2 hr, whereas six additional dogs (group C) served as control. During baseline conditions, Pi approximated LAP in both groups. In group C dogs, increasing LAP at constant Q degrees led to a proportional augmentation of Ppa. In group E dogs, endotoxin resulted in a shift of the Ppa-Q relationships to higher pressures due to both increases in Pi and slope. In addition, changes in Pla over the same range as in control dogs affected Ppa only at the highest levels of Pla. We conclude that endotoxin insult increases the critical closing pressure that exceeds Pla and induces the occurrence of a Starling resistor responsible for the production of an effective vascular waterfall. [less ▲]

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See detailDestruction of Stearic Acid Nitroxyl Radicals Mediated by Photoexcited Merocyanine 540 in Liposomal and Micellar Systems
Hoebeke, Maryse ULg; Seret, Alain ULg; Piette, Jacques ULg et al

in Biochemistry (1993), 32(10), 2730-2736

Fatty acid spin labels have been included into liposomes and micelles, in order to study the photochemical behavior of merocyanine 540 toward nitroxyl radicals situated at various depths in the bilayer or ... [more ▼]

Fatty acid spin labels have been included into liposomes and micelles, in order to study the photochemical behavior of merocyanine 540 toward nitroxyl radicals situated at various depths in the bilayer or the surfactant layer. Visible illumination of the dye, either free in ethanol or bound to liposomes or micelles, leads to the reduction of the electron spin resonance signal of the label. The efficiency of the interaction between merocyanine 540 and spin labels depends on the depth at which the nitroxyl moiety is localized in the micelle or vesicle. Fluorescence measurements indicate that the first excited singlet state of merocyanine 540 is not directly implicated in the reaction mechanism. Flash photolysis experiments conducted in aqueous solutions of hexadecyltrimethylammonium bromide micelles show that the presence of nitroxyl radical decreases the rate constant of triplet decay in a concentration-dependent fashion. The corresponding quenching rate constant (kq) is determined for the different spin labels. The kq values and the reduction rates of ESR signal show the same dependence on the localization of the nitroxyl moiety in the micelles. [less ▲]

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See detailSmall-angle X-ray scattering study of carboxylato-telechelic poly(tert-butyl acrylate)s
Fontaine, Frédéric; Ledent, Jean; Sobry, Roger et al

in Macromolecules (1993), 26(6), 1480-1482

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See detailAnionic polymerization of acrylic monomers. 10. 13C and 7Li NMR studies on the monomeric model of living poly(methyl)methacrylate)
Wang, Jin-Shan; Jérôme, Robert ULg; Warin, R. et al

in Macromolecules (1993), 26(6), 1402-1406

A monomeric model structurally comparable to the active end of a living poly(methyl methacrylate) (PMMA), i.e., a methyl α-lithioisobutyrate (MIBLi)/THF solution, was studied by both Li-7 and C-13 NMR. It ... [more ▼]

A monomeric model structurally comparable to the active end of a living poly(methyl methacrylate) (PMMA), i.e., a methyl α-lithioisobutyrate (MIBLi)/THF solution, was studied by both Li-7 and C-13 NMR. It has been shown that two different coordination aggregates exist in a MEBLi/THF solution. That situation corresponds to a slow exchange process between tetramer and dimer, i.e. the later being favored by a decrease in temperature or concentration. Such an equilibrium is characterized with ΔH = -2.1 kcal/mol and ΔS = -13.7 cal/mol.K by means of Li-7 NMR. Again, C-13 NMR results obtained suggest that a MIBLi/THF solution displays a charge-delocalized character, which is different from a O-Li-bonded bonded structure in solid state established by crystal analyses of several similar ester enolates. [less ▲]

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See detailImmortalization of Human Cervical Keratinocytes by Human Papillomavirus Type 33
Gilles, Christine ULg; Piette, Jacques ULg; Rombouts, S. et al

in International Journal of Cancer = Journal International du Cancer (1993), 53(5), 872-9

Ten immortalized cell lines were established by transfection of human cervical keratinocytes (CK) with HPV-33 DNA and some of their characteristics were investigated. The following observations were made ... [more ▼]

Ten immortalized cell lines were established by transfection of human cervical keratinocytes (CK) with HPV-33 DNA and some of their characteristics were investigated. The following observations were made: (a) several cell lines have reached over 100 population doublings in vitro; (b) 3 transcripts were observed, 2 being encoded by the E6/E7 open reading frames (ORFs); (c) cytogenetic analyses showed important genetic modifications such as aneuploidy and isochromosome formation of the q arm of chromosome 8; (d) 2 of the 10 cell lines developed colonies in soft agar but none was able to form tumors when injected s.c. into nude mice; (e) Southern analysis suggested that a single copy of HPV-33 is integrated at a single common site within the genome of the 10 cell lines. These immortalized cell lines should be useful for studying mechanisms involved in proliferation, differentiation and neoplastic transformation of CK by HPV-33. [less ▲]

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See detailThe Catecholaminergic System of the Quail Brain: Immunocytochemical Studies of Dopamine Beta-Hydroxylase and Tyrosine Hydroxylase
Bailhache, T.; Balthazart, Jacques ULg

in Journal of Comparative Neurology (The) (1993), 329(2), 230-56

The distribution of dopamine beta-hydroxylase and tyrosine hydroxylase, two key enzymes in the biosynthesis of catecholamines, was investigated by immunocytochemistry in the brain of male and female ... [more ▼]

The distribution of dopamine beta-hydroxylase and tyrosine hydroxylase, two key enzymes in the biosynthesis of catecholamines, was investigated by immunocytochemistry in the brain of male and female Japanese quail. Cells or fibers showing dopamine beta-hydroxylase and tyrosine hydroxylase immunoreactivity were considered to be noradrenergic or adrenergic, while all structures showing only tyrosine hydroxylase immunoreactivity were tentatively considered to be dopaminergic. The major dopaminergic and noradrenergic cell groups that have been identified in the brain of mammals could be observed in the Japanese quail, with the exception of a tuberoinfundibular dopaminergic group. The dopamine beta-hydroxylase-immunoreactive cells were found exclusively in the pons (locus ceruleus and nucleus subceruleus ventralis) and in the medulla (area of the nucleus reticularis). The tyrosine hydroxylase-immunoreactive cells had a much wider distribution and extended from the preoptic area to the level of the medulla. They were, however, present in larger numbers in the area ventralis of Tsai and in the nucleus tegmenti pedunculo-pontinus, pars compacta, which respectively correspond to the ventral tegmental area and to the substantia nigra of mammals. A high density of dopamine beta-hydroxylase- and tyrosine hydroxylase-immunoreactive fibers and punctate structures was found in several steroid-sensitive brain regions that are implicated in the control of reproduction. In the preoptic area and in the region of the nucleus accumbens-nucleus stria terminalis, immunonegative perikarya were completely surrounded by immunoreactive fibers forming basket-like structures. Given that some of these cells contain the enzyme aromatase, these structures may represent the morphological substrate for a regulation of aromatase activity by catecholamines. The dopamine beta-hydroxylase-immunoreactive fibers were also present in a larger part of the preoptic area of females than in males. This sex difference in the noradrenergic innervation of the preoptic area presumably reflects the sex difference in norepinephrine content in this region. [less ▲]

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See detailEconomic, Clinical and Functional Consequences of a Treatment Using Metrenperone During an Outbreak of Shipping Fever in Cattle
Genicot, Bruno; Mouligneau, Frédéric; Rollin, Frédéric ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1993), 132(10), 245-247

Eighteen of 91 seven- to nine-month-old Belgian white and blue double-muscled male fattening cattle developed typical signs of shipping fever. They were all injected intramuscularly once a day for three ... [more ▼]

Eighteen of 91 seven- to nine-month-old Belgian white and blue double-muscled male fattening cattle developed typical signs of shipping fever. They were all injected intramuscularly once a day for three days with 5 mg/kg of enrofloxacin, and in addition nine selected at random were injected intramuscularly five times at 12 hour intervals with 0.1 mg/kg of metrenperone, a 5-hydroxytryptamine blocker, the other nine receiving a placebo. During the outbreak of shipping fever metrenperone showed effective antipyretic properties, and all the calves treated with it made a complete recovery. Moreover, during the 360 day fattening period following the outbreak, the cattle treated with metrenperone gained on average 45.4 kg more weight than the control cattle. [less ▲]

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See detailHIV-1 promoter activation following an oxidative stress mediated by singlet oxygen
Legrand, Sylvie ULg; Hoebeke, Maryse ULg; Vaira, Dolorès ULg et al

in Journal of Photochemistry and Photobiology B : Biology (1993), 17(3), 229-237

Various biological processes, such as photosensitization or inflammatory reactions, can generate singlet oxygen (O-1(2)) as one of the major oxidative species. Because this oxidant can be generated either ... [more ▼]

Various biological processes, such as photosensitization or inflammatory reactions, can generate singlet oxygen (O-1(2)) as one of the major oxidative species. Because this oxidant can be generated either extracellularly or intracellularly, it can cause severe damage to various biological macromolecules, even to those deeply embedded inside the cells such as DNA. Sublethal biological modifications induced by different DNA-damaging agents can promote various cellular responses initiated by the activation of various cellular genes and certain heterologous viruses. Since O-1(2) fulfils essential prerequisites for a genotoxic substance, we have examined the effects of an oxidative stress, mediated by this species, on cells harbouring a heterologous promoter-leader sequence derived from the human immunodeficiency virus type 1 (HIV-1). Our results demonstrate that HIV-1 long terminal repeat (LTR), integrated into the cellular I)NA of epithelial cells, can be transactivated following an oxidative stress mediated by O-1(2). In addition, using HIV-1 latently infected promonocytes or lymphocytes, it can be shown that virus reactivation can be induced through a sublethal dose of O-1(2) generated intracellularly. An extracellular generation of O-1(2) can promote a substantial lethal effect without HIV-1 reactivation. These data may be relevant to the understanding of the events converting a latent infection into a productive one and to the appearance of the acquired immune deficiency syndrome. [less ▲]

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See detailBehavioral sensitization and tolerance to the D-sub-2 agonist RU 24213 : dissociation between several patterns in mice
Tirelli, Ezio ULg; Jodogne, C.

in Pharmacology, Biochemistry & Behavior (1993), 44(3), 627-632

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See detailL'angoisse minimaliste
Demoulin, Laurent ULg

in Indications. La Revue des Romans (1993), 1(50), 27-31

Description de quelques caractéristiques du roman français contemporain.

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See detailSequential anti-core glycolipid immunoglobulin antibody activities in patients with and without septic shock and their relation to outcome
Nys, Monique ULg; Damas, Pierre ULg; Joassin, Luc et al

in Annals of Surgery (1993), 217(3), 300-306

OBJECTIVE: This study follows the sequential changes in anti-lipopolysaccharide antibodies in infected patients with and without septic shock. SUMMARY BACKGROUND DATA: A relation between high endogenous ... [more ▼]

OBJECTIVE: This study follows the sequential changes in anti-lipopolysaccharide antibodies in infected patients with and without septic shock. SUMMARY BACKGROUND DATA: A relation between high endogenous levels of anti-LPS antibodies and protection against bacteremia and septic shock in at-risk patient groups has been observed. However, information on the daily follow-up and kinetics of apparition or disappearance of anti-LPS antibody activities and their relations with the protective properties of the different immunoglobulin classes has not been clearly investigated. METHODS: Two hundred and five septic surgical patients were studied during their stay in the intensive care unit during a period of 3 years. Among these patients, septic shock developed in 54 and 47 died. A sensitive ELISA was used to study circulating IgM and IgG antibodies to the core glycolipid (CGL) region of Salmonella minnesota R595. The activities were measured each day when sepsis occurred and every hour during septic shock. RESULTS: Anti-CGL IgM activity was found in 32% of the septic patients. This response, however, most often appeared to be transient. A strong correlation was observed between the occurrence of septic shock and the absence of anti-CGL IgM activity on admission to the ICU (p < 0.02). Anti-CGL IgG activity was detected in 82% of the patients and better correlated with outcome for patients with high or rising activities during their hospitalization (p < 0.0005). In patients with septic shock or irreversible organ failure, a fall in the anti-CGL IgG activity was observed before death, suggesting that the IgG antibodies were consumed during this acute event. Therefore, the anti-CGL IgG activity measured by ELISA could be used as a marker of the evolution of the illness. CONCLUSIONS: Our observations demonstrate the interest to follow-up the evolution of the anti-CGL antibodies during sepsis. The fall of these antibodies during septic shock and in patients who died was an additional argument to perform, as an additive form, passive antibody therapy to decrease lethality in this group of patients. [less ▲]

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See detailBrain Aromatase and the Control of Male Sexual Behavior
Balthazart, Jacques ULg; Foidart, Agnès ULg

in Journal of Steroid Biochemistry & Molecular Biology (1993), 44(4-6), 521-40

The activational effects of testosterone (T) on male copulatory behavior are mediated by its aromatization into estradiol. In quail, we have shown by stereotaxic implantation of steroids and metabolism ... [more ▼]

The activational effects of testosterone (T) on male copulatory behavior are mediated by its aromatization into estradiol. In quail, we have shown by stereotaxic implantation of steroids and metabolism inhibitors and by electrolytic lesions that the action of T and its aromatization take place in the sexually dimorphic medial preoptic nucleus (POM). The distribution and regulation of brain aromatase was studied in this species by product-formation assays measuring aromatase activity (AA) in microdissected brain regions and by immunocytochemistry (ICC). Aromatase-immunoreactive (ARO-ir) neurons were found in four brain regions: the POM, the septal region, the bed nucleus of the stria terminals (BNST) and the tuberal hypothalamus. ARO-ir cells actually outline the POM boundaries. ARO-ir material is found not only in the perikarya of neurons but also in the full extension of their cellular processes including the axons and the presynaptic boutons. This is confirmed at the light level by the demonstration of immunoreactive fibers and punctate structures in brain regions that are sometimes fairly distant from the closest ARO-ir cells. A lot of ARO-ir cells in the POM and BNST do not contain immunoreactive estrogen receptors (ER-ir) as demonstrated by double label ICC. These morphological data suggest an unorthodox role for the enzyme or the locally formed estrogens. In parallel with copulatory behavior, the preoptic AA decreases after castration and is restored by T to levels seen in sexually mature males. This probably reflects a change in enzyme concentration rather than a modulation of the activity in a constant number of molecules since the maximum enzymatic velocity (Vmax) only is affected while the affinity (Km) remains unchanged. In addition, T increases the number of ARO-ir neurons in POM and other brain areas suggesting that the concentration of the antigen is actually increased. This probably involves the direct activation of aromatase transcription as demonstrated by RT-PCR studies showing that aromatase mRNA is increased following T treatment of castrates. These activating effects of T seem to result from a synergistic action of androgenic and estrogenic metabolites of the steroid. The anatomical substrate for these regulations remains unclear at present especially in POM where ARO-ir cells do not in general contain ER-ir while androgen receptors appear to be rare based on both [3H] dihydrotestosterone autoradiography and ICC. Transynaptic mechanisms of control may be considered. A modulation of brain aromatase by catecholamines is also suggested by a few pharmacological studies.(ABSTRACT TRUNCATED AT 400 WORDS) [less ▲]

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See detailComparative immunohistochemical study of herpes-simplex and varicella-zoster infections
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1993), 422(2), 121-126

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new ... [more ▼]

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387-and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis. [less ▲]

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See detailSobre a construção de porjetores ortogonais
Iemma, A. F.; Palm, Rodolphe ULg; Claustriaux, Jean-Jacques ULg

in Revista de Matemática e Estatìstica (1993), 11

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See detailBarium isotopes in the very metal-poor star HD 140283
Magain, Pierre ULg; Zhao, G.

in Astronomy and Astrophysics (1993), 268

The fractions of odd and even isotopes of barium in the metal-poor star HD140283 are determined from an analysis of the profile of the Ba II resonance line, taking advantage of the differences in ... [more ▼]

The fractions of odd and even isotopes of barium in the metal-poor star HD140283 are determined from an analysis of the profile of the Ba II resonance line, taking advantage of the differences in hyperfine structure splitting between the different isotopes. Although one would expect an increased contribution of the r-process in very metal-poor stars, it is found that the isotopic ratio is very close to solar. [less ▲]

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See detailMagnitudes des étoiles doubles.
Renson, Pierre ULg

in Ciel et Terre (1993), 109

Not Available

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See detailEvaluation quantitative de l'érythème par colorimétrie. Effets de l'histamine de corticostéroïdes et d'extraits végétaux
Arrese Estrada, Jorge ULg; Nikkels, Arjen ULg; El Fekih, N. et al

in Journal d'Actualités Médicales Belges (1993), 5

L'évaluation de l'érythème est difficile et souvent aléatoire en clinique. Afin d'étudier la couleur des téguments, la colorimétrie est proposée depuis quelques années. Elle permet plus de rigueur. En ... [more ▼]

L'évaluation de l'érythème est difficile et souvent aléatoire en clinique. Afin d'étudier la couleur des téguments, la colorimétrie est proposée depuis quelques années. Elle permet plus de rigueur. En tous cas, elle permet d'aligner des chiffres dont les variations peuvent être étudiées dans le temps. Le présent travail a permis de tester, de "calibrer" la méthodologie colorimétrique dans le domaine de l'érythème cutané. D'une part, l'effet vasodilatateur (érythémateux) de l'histamine, de l'autre, le blanchiment induit par l'application de topiques corticoïdes et phytopharmaceutiques ont été mesurés et discutés. [less ▲]

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See detailStrychnopentamine, a Potential Anticancer Agent
Quetin-Leclercq, J.; Bouzahzah, B.; Pons, A. et al

in Planta Medica (1993), 59(1), 59-62

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg ... [more ▼]

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg strychnopentamine (1 per day) induce a significant decrease of the number of tumor cells and a significant increase of the survival of the treated mice. Observed side effects are partial haemolysis and some liver damage. [less ▲]

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See detailLas cosas escritas en la Nueva España
Vanden Berghe, Kristine ULg

in L'ordinaire. Mexique Amérique Centrale (1993), 143

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See detailBrachymorphism-Onychodysplasia-Dysphalangism Syndrome
Verloes, Alain ULg; Bonneau, D.; Guidi, O. et al

in Journal of Medical Genetics (1993), 30(2), 158-61

Three unrelated children are reported with intrauterine proportionate growth retardation and facial dysmorphism (broad nose, flat malar area, large mouth, pointed chin), microcephaly, hypo/aplasia of the ... [more ▼]

Three unrelated children are reported with intrauterine proportionate growth retardation and facial dysmorphism (broad nose, flat malar area, large mouth, pointed chin), microcephaly, hypo/aplasia of the terminal fifth digits, and (sub)normal intelligence. Radiological findings include hypo/aplasia or fusion of the distal phalanges of the fifth finger and toe, brachymesophalangism V, and nail dysplasia or aplasia. One child had cystic adenomatoid disease of the lung. The pattern of anomalies presented by these children closely resembles a syndrome incompletely delineated in 1971 by Senior in six children, which has often been considered to be a mild form of Coffin-Siris syndrome. We suggest that this is an independent entity (BOD syndrome). The aetiology is still unknown. Differential diagnosis and nosological difficulties are discussed. [less ▲]

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See detailEfficient Immunoselection of Cytolytic Effectors with a Magnetic Cell Sorter
Jacobs, Nathalie ULg; Moutschen, Michel P; Boniver, Jacques ULg et al

in Research in Immunology (1993), 144(2), 141-50

This paper describes a rapid and efficient method for the sorting of in vitro activated cytolytic effectors cells. For cytotoxic assays, a large number of cells with conserved function must be rapidly ... [more ▼]

This paper describes a rapid and efficient method for the sorting of in vitro activated cytolytic effectors cells. For cytotoxic assays, a large number of cells with conserved function must be rapidly obtained. Immunomagnetic sorting was chosen because it is faster than flow cytometry sorting. The MACS system requires the use of paramagnetic beads of small diameter (100-150 nm), reputed to interfere minimally with cell function. In order to generate the cytolytic effectors, peripheral blood lymphocytes were cultivated in the presence of interleukin-2 (50 U/ml) and anti-CD3 monoclonal antibody (BMA030, 100 ng/ml) for 4 days. Cell separation was based on the membrane expression of the CD3 complex. The purity obtained for positive (CD3+) cell sorting with the MACS was higher than 95%. The purity of negative (CD3-) cell fraction was more variable, but further purification by flow cytometry rapidly yielded purity higher than 95%. Cytotoxic assays were performed against four target cell lines (K562, Daudi, HL60 and U937) and proliferation assays showed that both negatively and positively selected populations had conserved their function acquired during culture in the presence of anti-CD3 mAb and IL2. [less ▲]

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See detailAspects immunologiques et hématologiques de l'infection par le virus HIV
Delhougne, B.; Fillet, Georges ULg

in Revue Médicale de Liège (1993), 48(2), 72-80

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See detailAn Unusual Case of Cor Triatriatum: Clinical Presentation and the Haemodynamics of a Rare Anomaly
Radermecker, M. A.; Grenade, Thierry ULg; Jalali, H. et al

in Cardiovascular Surgery (1993), 1(1), 83-5

The case of a 24-year-old woman with cor triatriatum sinister and a persistent left superior vena cava connected to the junction of the left upper pulmonary vein and left atrium is reported. A diagnosis ... [more ▼]

The case of a 24-year-old woman with cor triatriatum sinister and a persistent left superior vena cava connected to the junction of the left upper pulmonary vein and left atrium is reported. A diagnosis of partial anomalous pulmonary venous return had been made and the patient underwent surgery. Inspection of the left atrium revealed a complex form of left triatrial heart. The general haemodynamics of the malformation are discussed, with particular emphasis on the features accounting for variation in the clinical presentation. This rare anomaly often remains undiagnosed before surgery and should be considered whenever obstruction of pulmonary venous flow above the mitral valve is not explained by pulmonary vein stenosis, mitral or mitral valve-linked abnormalities, atrial tumours or anomalous pulmonary venous drainage with high resistance to flow. [less ▲]

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See detailMultiple System Organ Failure after open-heart surgery in infants and children
Seghaye, Marie-Christine ULg; Engelhardt, W.; Grabitz, R. G. et al

in Thoracic and Cardiovascular Surgeon (1993), 41(1), 49-53

Between January 1985 and March 1989 we retrospectively observed Multiple System Organ Failure (MSOF) in 16 of 460 children (3.5%) who underwent cardiac surgery with cardiopulmonary bypass for congenital ... [more ▼]

Between January 1985 and March 1989 we retrospectively observed Multiple System Organ Failure (MSOF) in 16 of 460 children (3.5%) who underwent cardiac surgery with cardiopulmonary bypass for congenital heart disease. MSOF was arbitrarily defined as a clinical entity with failure of two or more vital organ systems associated with high fever, thrombocytopenia, and cardiocirculatory insufficiency and occurring within the first postoperative week. In 13 children the first clinical manifestations of MSOF were evident on the first postoperative day and in the other 3 on the second or third postoperative day. All children showed acute renal failure, acute hepatic failure, high fever, and thrombocytopenia. Most of them showed respiratory insufficiency and neurological involvement. Seven of the 16 children died. Four of the 9 surviving patients had neurological sequelae still present 6 months after the operation, and the others recovered completely. [less ▲]

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See detailCloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10
Arpigny, J. L.; Feller, Georges ULg; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1993), 1171(3), 331-3

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M ... [more ▼]

A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold. [less ▲]

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See detailAutres médiateurs de l'inflammation et du sepsis
Lamy, Maurice ULg; Deby, Ginette ULg; Damas, Pierre ULg

in Revue du Praticien (La) (1993), 43(1), 19-25

During sepsis, the systemic inflammatory response is characterized by the release of numerous mediators supporting and dispersing inflammation. In Gram negative sepsis, the endotoxins play a starting role ... [more ▼]

During sepsis, the systemic inflammatory response is characterized by the release of numerous mediators supporting and dispersing inflammation. In Gram negative sepsis, the endotoxins play a starting role, while in other sepsis, the triggering mediators or mechanisms are unknown but lead to a similar inflammatory reaction. Coagulation and complement cascades are activated, with the release of chemoattractive substances, mediators and proteases and the activation of phagocytic cells. Macrophages/monocytes and polymorphonuclear leucocytes produce then active oxygen species and cytokines; they degranulate (releasing active enzymes such as myeloperoxidase), they express an increasing number of membrane receptors able to interact with endothelial cells and release a supplementary lot of inflammatory mediators (prostanoids, platelet activating factor, leukotrienes ... ). Platelets, also activated, produce the same mediators (TXA2, PAF ...) or specific ones such as serotonine, platelet factor 4, growth factors. Last, but not least, the endothelial cells are stimulated, directly (by endotoxins) or undirectly (by cytokines, C5a, PAF ...). These cells play then a main role by their own phagocytic activity, by alteration of their antithrombotic and fibrinolytic potential, by their secretion of inflammatory mediators and by an increased expression of receptors of adhesivity for the activated phagocytes or platelets, what leads to endothelium injury with membrane permeability alterations. These cascades of activation, these extensions of the inflammatory reaction by the mediators and by the phagocytes and platelets can explain the frequency of multiple organ failure during sepsis as well as the difficulty of an adequate pharmacological therapy. [less ▲]

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See detailA first look at the ASSI ultraviolet results
Chakrabarti, S.; Gladstone, G. R.; Tobiska, W. K. et al

in Advances in Space Research (1993), 13

The Airglow and Solar Spectrometer Instrument (ASSI) on the San Marco D satellite has obtained near-simultaneous measurements of solar irradiances and airglow emissions in the 200-7000 A spectral region ... [more ▼]

The Airglow and Solar Spectrometer Instrument (ASSI) on the San Marco D satellite has obtained near-simultaneous measurements of solar irradiances and airglow emissions in the 200-7000 A spectral region. The satellite was placed in an equatorial, elliptical orbit on 25 March 1988, which permitted observations of airglow emissions in the 280-600-km altitude range at various local times. The instrument complement on the satellite provides an opportunity both for self-consistent examination of the excitation mechanisms of various airglow features and for constraining model parameters. An overview of the data obtained by ASSI is presented along with preliminary modeling results of the UV airglow. [less ▲]

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See detailVivre avec un nouveau coeur
Triffaux, Jean-Marc ULg

in Revue Médicale de Liège (1993), 48(1), 19-25

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See detailA novel mutation in the proteolipid protein gene leading to Pelizaeus-Merzbacher disease.
Otterbach, B.; Stoffel, W.; RAMAEKERS, Vincent ULg

in Biological chemistry Hoppe-Seyler (1993), 374(1), 75-83

Point mutations of the gene of human proteolipid protein (PLP) have been recognized as the molecular basis of one form of leukodystrophy, the X-chromosome-linked Pelizaeus-Merzbacher disease (PMD). We ... [more ▼]

Point mutations of the gene of human proteolipid protein (PLP) have been recognized as the molecular basis of one form of leukodystrophy, the X-chromosome-linked Pelizaeus-Merzbacher disease (PMD). We report the molecular analysis of four PMD patients in three unrelated families and describe a point mutation (G-->A transition) in exon V which leads to the substitution of Gly216 by a serine residue in a highly conserved extracytosolic domain and a Mae I RFLP. Molecular modelling with energy minimization indicates that this seemingly minor alteration of the amino-acid sequence induces a considerable conformational change and tight packing of the polypeptide chain apparently not compatible with the regular PLP function in oligodendrocytes. This mutation has been detected and characterized by PCR amplification of genomic DNA using intron and exon primers and the complete sequence analysis of the seven exons and a 300 bp promoter region of the PLP gene of two affected brothers. The sequence analysis of a PCR fragment representing exon V amplified from genomic DNA of different kindreds of the pedigree revealed the mother as the only carrier indicating that the mutation has occurred de novo in the mother's germline. PLP gene (including the 8.8 kb intron I) rearrangements have been excluded by Southern blot hybridization and overlapping PCR amplification of genomic DNA. [less ▲]

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See detailPhotophysical investigation of Rose Bengal in aqueous sodium taurocholate solutions
Seret, Alain ULg; Van de Vorst, Albert

in Journal of Photochemistry and Photobiology B : Biology (1993), 17(1), 47-56

The photophysics of rose bengal has been studied in aqueous sodium taurocholate solutions with or without 0.6 mol NaCl dm−3. The ground and first excited singlet states solubilize only in the aggregates ... [more ▼]

The photophysics of rose bengal has been studied in aqueous sodium taurocholate solutions with or without 0.6 mol NaCl dm−3. The ground and first excited singlet states solubilize only in the aggregates formed by sodium taurocholate, whereas the first triplet state solubilizes both in the bulk phase and in the aggregates. A lower limit of the corresponding entry and exit rate constants ke and ks, respectively has been estimated: ke ≥ 5 X 106 dm3 mol−1 s−1 (2x107 dm3 mol−1 s−1 with 0.6 mol NaCl dm−3) and ks ≥ 105 s−1. The triplet state as well as the semioxidized radical react with the taurocholate anion. The former reaction leads to the formation of semireduced dye. The latter is responsible for the detection of only semioxidized radicals which move freely in the aqueous phase. The results are compared with those obtained previously in aqueous sodium dodecyl sulphate solutions and the possible biological implication is discussed. [less ▲]

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See detailPlasma Na-K ATPase inhibitor activity and intracellular ions during hemodialysis
Krzesinski, Jean-Marie ULg; Du, F.; Pequeux, M. L. et al

in International Journal of Artificial Organs (1993), 16(1), 23-30

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic ... [more ▼]

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic controls (NC). As compared with the NC population, significantly enhanced values for erythrocyte (RBC) Na, Ca, platelet cytosolic Ca and EDLS were observed in all the uremic patients tested just before a dialysis session, as well as a decrease in RBC Ca ATPase and in the platelet pH. In uremia, significant correlations have been noted between RBC Na and platelet Ca (r = 0.6) or systolic BP (r = 0.45); between platelet Ca and systolic blood pressure (r = 0.8) or diastolic BP (r = 0.5) and between EDLS and RBC Na, Ca or platelet Ca (r = 0.5). Anti-hypertensive treatment has no influence on these parameters. During dialysis, a significant decrease has been noted in RBC Na, Ca, platelet Ca, SBP (only in untreated patients) and EDLS and an increase in RBC Ca ATPase and platelet pH. These modifications are significantly correlated with the weight change. [less ▲]

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See detailPaget's Disease of Bone Treated with a Five Day Course of Oral Tiludronate
Reginster, Jean-Yves ULg; Lecart, Marie-Paule ULg; Deroisy, Rita ULg et al

in Annals of the Rheumatic Diseases (1993), 52(1), 54-7

Chloro-4-phenyl thiomethylene bisphosphonate (tiludronate) is a new drug which can be used as an inhibitor of bone resorption. As it remains in bone for a long time, and as mineralisation defects have ... [more ▼]

Chloro-4-phenyl thiomethylene bisphosphonate (tiludronate) is a new drug which can be used as an inhibitor of bone resorption. As it remains in bone for a long time, and as mineralisation defects have only been seen at doses much higher than those required to decrease osteoclastic activity, it could be given at high doses over a short period of time. Eighteen patients with Paget's disease of bone were randomly allocated to three therapeutic groups receiving respectively 600, 800, and 1200 mg/day tiludronate for five days. Serum alkaline phosphatase activity and the urinary hydroxyproline/creatinine ratio were quickly and drastically reduced in all three groups. A significant reduction of serum alkaline phosphatases and the hydroxyproline/creatinine ratio was still present six months after the five day therapeutic course, reflecting a sustained activity of tiludronate even after stopping treatment. Dose dependent short and long term reductions of bone turnover rate were observed. Biochemical assessment of haematological, renal, or hepatic tolerance did not show any toxicity of tiludronate. Fifty per cent of patients treated by a dose of 1200 mg/day reported gastrointestinal disturbances, however, making this dosage unsuitable for clinical practice. [less ▲]

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See detailMisdiagnosed Malignancy in Transplanted Organs
Detry, Olivier ULg; Detroz, Bernard ULg; D'Silva, M. et al

in Transplant International (1993), 6(1), 50-4

The case reports of three patients who received cancer-bearing organs at this institution are presented. A fourth recipient, who was to be transplanted with a cancerous kidney, was spared this disastrous ... [more ▼]

The case reports of three patients who received cancer-bearing organs at this institution are presented. A fourth recipient, who was to be transplanted with a cancerous kidney, was spared this disastrous complication. The relevant data regarding the donors is also alluded to, with special reference to the type and site of the primary malignancy. Following these case reports, the implications of these issues, their possible prevention, and further management are discussed. [less ▲]

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See detailIdentification of Different Target Glycoproteins for Bovine Herpes Virus Type 1-Specific Cytotoxic T Lymphocytes Depending on the Method of in Vitro Stimulation
Denis, M.; Slaoui, M.; Keil, G. et al

in Immunology (1993), 78(1), 7-13

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from ... [more ▼]

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated from immune cattle. Peripheral blood mononuclear cells (PBMC) expanded in vitro in the presence of interleukin-2 (IL-2) and lysed both gIII- and gIV-infected target cells. Secondary in vitro stimulation of PBMC was also performed in the presence of either fixed BHV-1-infected autologous fibroblasts or ultraviolet (UV)-inactivated virus. Both methods of antigen presentation allowed the proliferation of BHV-1-specific CTL but the target glycoprotein for these CTL differed depending on the method of stimulation. Vaccinia-gIV-infected targets were lysed predominantly when PBMC were stimulated by fixed infected fibroblasts, whilst PBMC stimulated by UV-inactivated virus lysed mostly vaccinia-gIII-infected targets. This observation could be explained by a different processing pathway of BHV-1 antigens in each cell type involved. [less ▲]

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See detailResults of metagnostic (system-oriented) and diagnostic (process-oriented) models
Beckers, Jean-Marie ULg; Haus, J.; Nihoul, Jacques ULg et al

in Progress in Belgian Oceanographic Research (1993)

After a review of the Mediterranean system, and the justification of the scientific interest, we examine the type of model (scope, purview and resolution) used for the description and simulation of the ... [more ▼]

After a review of the Mediterranean system, and the justification of the scientific interest, we examine the type of model (scope, purview and resolution) used for the description and simulation of the Mediterranean circulation from a system-oriented view and a process-oriented view. These two aspects of mathematical simulations are then illustrated by two examples: a) The simulation of the month to month variability of the general circulation in the Western Mediterranean Sea shows the main physical features, but the choice of initial conditions is crucial for a realistic simulation, and an inverse model is proven to be absolutely necessary for a valuable prognostic model. b) Secondly, a high resolution simulation of the Algerian Current instability is performed. It is well known that the Atlantic water flows along the Algerian coast as a light water intrusion. This current is unstable, and mesoscale activities generate cyclones and anticyclones, but only the latter grow enough to get separated from the mean flow. Numerical simulations, in an idealized case, and reality show strong instabilities that are analyzed by energy budgets. It is suggested that the instabilities are primarily baroclinic, which is consistent with initial vertical movements at the front. [less ▲]

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See detailComparison between Alprazolam and Hydroxyzine for Oral Premedication
Franssen, Colette ULg; Hans, Pol ULg; Brichant, Jean-François ULg et al

in Canadian Journal of Anaesthesia = Journal Canadien d'Anesthésie (1993), 40(1), 13-7

The safety and efficacy of alprazolam and hydroxyzine administered orally as surgical premedicants were compared in a double-blind controlled study. Sixty-five patients were given either alprazolam 1 mg ... [more ▼]

The safety and efficacy of alprazolam and hydroxyzine administered orally as surgical premedicants were compared in a double-blind controlled study. Sixty-five patients were given either alprazolam 1 mg or hydroxyzine 75 mg, one to two hours before surgery. Anxiety was assessed by both the patient and the anaesthetist, the patient using a visual analogue scale, the anaesthetist employing both analogue and ordinal ratings. Sedation was assessed by the anaesthetist only, using the same two methods. Amnesia was appraised with a simple memory test. Safety was assessed by recording adverse effects and measuring haemodynamic variables. Premedication with alprazolam produced a modest reduction in anxiety (28%) (P < 0.01) while hydroxyzine had no detectable effect. The comparison of the sedation level and of the memory test revealed no difference between the two premedicants. Minor side effects were only observed in the hydroxyzine group. Changes in blood pressure were more pronounced in the hydroxyzine group. This study shows that alprazolam and hydroxyzine are safe and efficient oral premedicants. However, alprazolam is preferable to hydroxyzine in terms of anxiolytic and adverse effects. [less ▲]

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See detailPulmonary Response to Intravenous Administration of 5-Hydroxytryptamine after Type-2 Receptor Blockade in Healthy Calves
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in American Journal of Veterinary Research (1993), 54(1), 168-73

The purpose of this study was to investigate the mechanism by which 5-hydroxytryptamine (5-HT) modifies respiratory function, specifically, hyperventilation, diffuse bronchoconstriction, and pulmonary ... [more ▼]

The purpose of this study was to investigate the mechanism by which 5-hydroxytryptamine (5-HT) modifies respiratory function, specifically, hyperventilation, diffuse bronchoconstriction, and pulmonary arterial hypertension in cattle. We determined whether the IV response to 5-HT in calves was attributable to stimulation of 5-HT2 receptors. Six healthy unsedated young bull calves of the Friesian (n = 4) and of the Belgian White and Blue (n = 2) breeds were used. A specific 5-HT2 antagonist (metrenperone, 0.05 mg/kg of body weight) was administered IM 30 minutes before the cattle were given a 5-minute IV 5-HT infusion. Pulmonary function values were registered before, during, and after the 5-HT challenge infusion. Minute volume increased significantly, because of an increase in respiratory rate. Conversely, lung dynamic compliance, total pulmonary resistance, and pulmonary arterial pressure were not changed. We concluded that in cattle, 5-HT-induced ventilatory response is not mediated through activation of 5-HT2 receptors. However, the 5-HT2 receptors are involved in 5-HT-induced broncho- and pulmonary vasoconstriction. [less ▲]

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See detailPour une didactique des sources audiovisuelles
Jadoulle, Jean-Louis ULg

in Histoire et Enseignement : Bulletin (1993), 43e année(n°4), 11-13

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See detailNew Immigrants and Democratic Society. Minority Integrations in Western Societies
Martiniello, Marco ULg

in International Migration Review (1993), 27(101), 204-205

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See detailPurification and Biochemical Characterization of Recombinant Human Placental Growth Hormone Produced in Escherichia Coli
Igout, Ahmed ULg; Van Beeumen, Jozef; Frankenne, Francis ULg et al

in Biochemical Journal (1993), 295(3), 719-724

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively ... [more ▼]

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively replaces in the maternal bloodstream. hGH- V cDNA has previously been cloned and isolated. Analysis of its nucleotide sequence has revealed a 191-residue protein, hPGH, differing from hGH at 13 positions. The calculated pI is more basic than that of the pituitary hormone. Here we have inserted hGH- VcDNA into the pIN-III-ompA3 plasmid in order to produce hPGH in its native form in Escherichia coli D1210. Expression of hGH- V cDNA in E. coli is significantly lower than that of hGH cDNA with the same expression system. The hPGH produced in E. coli was purified in quantities sufficient to allow its biochemical and immunochemical characterization. The molecular mass of the protein was determined by electrospray m.s. The determined mass, 22320 Da, agrees well with the molecular mass calculated from the translated cDNA sequence, assuming the presence of two disulphide bridges. Having established the technique for producing hPGH with a primary structure identical to the natural, non-glycosylated, 22 kDa isoform, we can now plan the full physicochemical and pharmaceutical characterization of this new hormonal entity. [less ▲]

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See detailStructure sociologique de l'élecotrat écologiste en Wallonie : une première exploration
Jacquemain, Marc ULg; Vandekeere, Michel; Doutrelepont, René

in Res Publica (1993), 25

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much ... [more ▼]

The paper describes the main characteristics of ecological constituency through results of surveys. It shows how ecologist voter differ sharply from the rest of the population beeing much younger and much more educated, e.a. [less ▲]

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See detailUn historique de la carte géologique de la Belgique
Boulvain, Frédéric ULg

in Professional paper Belgian geological survey (1993), 262

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