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See detailIdentification and characterization of in vivo attenuated mutants of Brucella melitensis.
Lestrate, P.; Delrue, R. M.; DANESE, Isabelle ULg et al

in Molecular Microbiology (2000), 38(3), 543-51

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within ... [more ▼]

Brucella melitensis 16M is a Gram-negative alpha2-proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. Signature-tagged mutagenesis (STM) was used to identify genes required for the in vivo pathogenesis of Brucella. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5 day passage in BALB/c mice. The attenuation of 18 mutants was confirmed using an in vivo competition assay against the wild-type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone-like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of Brucella virulence. [less ▲]

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See detailCoumarinic derivatives as mechanism-based inhibitors of α-chymotrypsin and human leukocyte elastase
Pochet, Lionel; Doucet, Caroline; Dive, Georges ULg et al

in Bioorganic & Medicinal Chemistry (2000), 8(6), 1489-1501

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See detailTectonic vs. shallow origin of geodetically inferred ground movements: an NE Ardenne (Belgium) case
Demoulin, Alain ULg; Vliegen, Bernard; Charlier, Robert ULg

in Earth and Planetary Science Letters (2000), 181(1-2), 217-228

In order to get a better insight into the temporal pattern of height change variations in a moderately seismic intraplate area, a 1 km long section has been weekly leveled from April, 21, 1997 to January ... [more ▼]

In order to get a better insight into the temporal pattern of height change variations in a moderately seismic intraplate area, a 1 km long section has been weekly leveled from April, 21, 1997 to January, 12, 1998 in NE Ardenne (Belgium). As this section is located very close to a small reservoir of 25 X 10(6) m(3), it also allowed the influence of lake level variations on the nearby ground motion to be studied. The measured height difference variations show a maximum amplitude of 3.45 mm, with weekly values not exceeding 1 mm. A two-dimensional (2D) finite element modeling confirms that waterload variations in the lake are the primary cause of movement of the section, inducing maximum ground subsidence of about 2 cm and seasonal tilting of 3-4 mu rads within a range of 2-3 km. We also show that a fault passively alters the spatial distribution of the waterload-dependent movements. The removal of this component from the observed ground motion leaves oscillating residual displacements characterized by an amplitude of 1-1.5 mm and a 'period' of about 2 months. We demonstrate that these displacements are independent of rainfall and probably of groundwater fluctuation too. Although most of the residual motions take place on the fault straddled by the leveling section, no credible mechanism can be found to ascribe these motions to a tectonic process. (C) 2000 Elsevier Science B,V. All rights reserved. [less ▲]

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See detailIntestinal maturation induced by spermine in young animals
Peulen, Olivier ULg; Deloyer, P; Grandfils, Christian ULg et al

in Livestock Production Science (2000), 66

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See detailRelationship between CNV topography and irritability in depression
Papart, Patrick ULg; WEERTS, Pascale ULg; Pinto, Emmanuel ULg et al

in Journal of Psychophysiology (2000), 14(Suppl 1),

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See detailFerroelectricity in PbTiO3 thin films: A first principles approach
Rabe, K. M.; Ghosez, Philippe ULg

in Journal of Electroceramics (2000), 4(2-3), 379-383

The ground-state ferroelectric distortion of PbTiO3 thin films is studied using a first-principles effective Hamiltonian to describe the structural energetics at a microscopic level. Under short-circuit ... [more ▼]

The ground-state ferroelectric distortion of PbTiO3 thin films is studied using a first-principles effective Hamiltonian to describe the structural energetics at a microscopic level. Under short-circuit electrical and zero-stress mechanical boundary conditions, (0 0 1) films as thin as one unit cell are found to support a stable perpendicular polarization. Size effects in the layer-by-layer ferroelectric distortion are discussed. The continuum limit of the first-principles effective Hamiltonian has the same form as the widely-used Landau-Ginzburg expression for the free energy near T-c, so that the present results can be directly related to those of previous phenomenological studies. In particular, a microscopic interpretation of the extrapolation length delta is proposed. [less ▲]

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See detailRole of Tir and Intimin in the Virulence of Rabbit Enteropathogenic Escherichia coli (REPEC) Serotype O103:H2
Marchès, O.; Nougayrede, J. P.; Boullier, S. et al

in Infection and Immunity (2000), 68

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See detailNeural mechanisms of antinociceptive effects of hypnosis.
Faymonville, Marie ULg; Laureys, Steven ULg; Degueldre, Christian ULg et al

in Anesthesiology (2000), 92(5), 1257-67

BACKGROUND: The neural mechanisms underlying the modulation of pain perception by hypnosis remain obscure. In this study, we used positron emission tomography in 11 healthy volunteers to identify the ... [more ▼]

BACKGROUND: The neural mechanisms underlying the modulation of pain perception by hypnosis remain obscure. In this study, we used positron emission tomography in 11 healthy volunteers to identify the brain areas in which hypnosis modulates cerebral responses to a noxious stimulus. METHODS: The protocol used a factorial design with two factors: state (hypnotic state, resting state, mental imagery) and stimulation (warm non-noxious vs. hot noxious stimuli applied to right thenar eminence). Two cerebral blood flow scans were obtained with the 15O-water technique during each condition. After each scan, the subject was asked to rate pain sensation and unpleasantness. Statistical parametric mapping was used to determine the main effects of noxious stimulation and hypnotic state as well as state-by-stimulation interactions (i.e., brain areas that would be more or less activated in hypnosis than in control conditions, under noxious stimulation). RESULTS: Hypnosis decreased both pain sensation and the unpleasantness of noxious stimuli. Noxious stimulation caused an increase in regional cerebral blood flow in the thalamic nuclei and anterior cingulate and insular cortices. The hypnotic state induced a significant activation of a right-sided extrastriate area and the anterior cingulate cortex. The interaction analysis showed that the activity in the anterior (mid-)cingulate cortex was related to pain perception and unpleasantness differently in the hypnotic state than in control situations. CONCLUSIONS: Both intensity and unpleasantness of the noxious stimuli are reduced during the hypnotic state. In addition, hypnotic modulation of pain is mediated by the anterior cingulate cortex. [less ▲]

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See detailHuitième campagne de fouilles à Tell Beydar
Van der Stede, Véronique ULg; Talon, Philippe

in Annales d'Histoire de l'Art et d'Archéologie (2000), XXII

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See detailComparison between Open and Closed Repair for Abdominal Aortic Aneurysms: A Word of Caution
Limet, Raymond ULg; CREEMERS, Etienne ULg

in Acta Chirurgica Belgica (2000), 100(1), 12-5

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See detailAssessment of postprandial hepatic glycogen synthesis from uridine diphosphoglucose kinetics in obese and lean non-diabetic subjects.
Paquot, Nicolas ULg; Schneiter, P.; Scheen, André ULg et al

in International Journal of Obesity & Related Metabolic Disorders (2000), 24(10), 1297-302

BACKGROUND: Obese patients are frequently characterized by insulin resistance and decreased insulin-mediated glycogen synthesis in skeletal muscle. Whether they also have impaired postprandial hepatic ... [more ▼]

BACKGROUND: Obese patients are frequently characterized by insulin resistance and decreased insulin-mediated glycogen synthesis in skeletal muscle. Whether they also have impaired postprandial hepatic glycogen synthesis remains unknown. AIM: To determine whether postprandial hepatic glycogen synthesis is decreased in obese patients compared to lean subjects. METHODS: Lean and obese subjects with impaired glucose tolerance were studied over 4h after ingestion of a glucose load. Hepatic uridine diphosphoglucose kinetics were assessed using 13C-galactose infusion, with monitoring of urinary acetaminophen-glucuronide isotopic enrichment to estimate hepatic glycogen kinetics. RESULTS: Estimated net hepatic glycogen synthesis amounted to 18.6 and 22.6% of the ingested load in lean and obese subjects, respectively. CONCLUSION: Postprandial hepatic glycogen metabolism is not impaired in non-diabetic obese subjects. [less ▲]

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See detailRenal Artery Occlusion Following Blunt Abdominal Trauma
Elen, Philippe ULg; Ozlu, E.; SakalihasanN, Natzi ULg et al

in Acta Chirurgica Belgica (2000), 100(3, May-Jun), 107-10

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See detailRadiation therapy alone or combined surgery and radiation therapy in squamous-cell carcinoma of the penis?
Zouhair, A; COUCKE, Philippe ULg; Jeanneret, W et al

in European Journal of Cancer (2000), 37(2), 198203

To assess the prognostic factors and the outcome in patients with squamous-cell carcinoma of the penis, a retrospective review of 41 consecutive patients with non-metastatic invasive carcinoma of the ... [more ▼]

To assess the prognostic factors and the outcome in patients with squamous-cell carcinoma of the penis, a retrospective review of 41 consecutive patients with non-metastatic invasive carcinoma of the penis, treated between 1962 and 1994, was performed. The median age was 59 years (range: 35–76 years). According to the International Union Against Cancer (UICC) 1997 classification, there were 12 (29%) T1, 24 (59%) T2, 4 (10%) T3 and 1 TX (2%) tumours. The N-classification was distributed as follows: 29 (71%) patients with N0, 8 (20%) with N1, 3 (7%) with N2 and 1 (2%) with N3. Forty-four per cent (n=18) of the patients underwent surgery: partial penectomy with (n=4) or without (n=12) lymph node dissection, or total penectomy with (n=1) or without (n=1) lymph node dissection. 23 patients were treated with radiation therapy alone, and all but 4 of the patients who were operated upon received postoperative radiation therapy (n=14). The median follow-up period was 70 months (range 20–331 months). In a median period of 12 months (range 5–139 months), 63% (n=26) of the patients relapsed (local in 18, locoregional in 2, regional in 3 and distant in 3). Local failure (stump in the operated patients, and the tumour bed in those treated with primary radiation therapy) was observed in 4 out of 16 (25%) patients treated with partial penectomy ±postoperative radiotherapy versus 14 out of 23 (61%) treated with primary radiotherapy (P=0.06). 15 (83%) out of 18 local failures were successfully salvaged with surgery. In all patients, 5- and 10-year survival rates were 57% (95% confidence interval (CI), 41–73%) and 38% (95% CI, 21–55%), respectively. The 5-year local and locoregional rates were 57% (95% CI, 41–73%) and 48% (95% CI, 32–64%), respectively. In patients treated with primary radiotherapy, 5- and 10-year probabilities of surviving with penis preservation were 36% (95% CI, 22–50%) and 18% (95% CI, 2–34%), respectively. In multivariate analyses, survival was significantly influenced by the N-classification, and surgery was the only independent factor predicting the locoregional control. We conclude that, in patients with squamous-cell carcinoma of the penis, local control is better in patients treated with surgery. However, there seems to be no difference in terms of survival between patients treated by surgery and those treated by primary radiotherapy ±salvage surgery, with 39% having organ preservation. [less ▲]

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See detailLa place de l'intervention de Ross (autogreffe pulmonaire) dans la chirurgie de la valve aortique
RADERMECKER, Marc ULg; GRENADE, Thierry ULg; Gach, J. et al

in Revue Médicale de Liège (2000), 55(1), 35-8

The pulmonary autograft operation consists of an aortic replacement using the autologous pulmonary valve. The pulmonary valve is substituted by a cryopreserved pulmonary homograft. This operation is in ... [more ▼]

The pulmonary autograft operation consists of an aortic replacement using the autologous pulmonary valve. The pulmonary valve is substituted by a cryopreserved pulmonary homograft. This operation is in fact a delicate double valve replacement whose benefits are linked to the viability of the new aortic substitute. The pulmonary autograft has superb hemodynamic features and very low thrombogenicity. The report of a selected observation offers the occasion of defining the current indications of this operation in aortic valve surgery. [less ▲]

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See detailPour une démarche de qualité dans la formation continuée des enseignants : rôle de l'évaluation
Giot, Bernadette ULg

in Point sur la Recherche en Education (Le) (2000), 21

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See detailL'artériopathie des membres inférieurs chez la personne âgée
Petermans, Jean ULg; Zicot, M.

in Angéiologie (2000), 52(1), 13-17

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See detailDistribution and epidermal growth factor receptor expression of primordial follicles in human ovarian tissue before and after cryopreservation.
Qu, Jian Ping; Godin, Pierre-Arnaud; NISOLLE, Michelle ULg et al

in Human Reproduction (2000), 15(2), 303-10

The freezing of ovarian tissue and the growth of immature oocytes from primordial follicles is an interesting concept in ovarian tissue transplantation and in-vitro fertilization. In this study, the ... [more ▼]

The freezing of ovarian tissue and the growth of immature oocytes from primordial follicles is an interesting concept in ovarian tissue transplantation and in-vitro fertilization. In this study, the morphology and distribution of primordial follicles were studied in ovarian tissue from 24 women before and after cryopreservation. Cryopreservation did not significantly change either the morphology or number per unit volume of morphologically normal follicles in frozen ovarian tissue. Primordial follicles were predominant, accounting for 78.6% and 82.6% of total follicles in fresh and frozen ovarian tissues respectively. The distribution of follicles was extremely uneven in ovarian tissue. A large variation in follicle numbers was observed in ovarian tissue samples from patient to patient, and even in the same patient, indicating that the number of follicles counted in one sample of ovarian tissue may not represent the number of follicles in other tissue samples. Ovarian tissue could be frozen in the form of strips instead of fragments for fast processing and better viability of ovarian tissue in cryopreservation. The number of follicles in ovarian tissue declined with the increasing age of the patients. An immunohistochemical study showed that immunoreactivity for the epidermal growth factor (EGF) receptor was detected in primordial follicles of adult ovarian tissue. EGF receptor staining was most intense in the oocytes of primordial follicles. Weak staining for EGF receptor was observed in some surrounding pregranulosa cells. Immunohistochemical staining for EGF receptor was also present in the stromal cells of ovarian tissue, but to a much lesser degree. There was no significant difference in the immunohistochemical staining for EGF receptor in ovarian tissue before and after cryopreservation. [less ▲]

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See detailPhalangeal osteosonogrammetry study: age-related changes, diagnostic sensitivity, and discrimination power. The Phalangeal Osteosonogrammetry Study Group.
Wuster, C; Albanese, C; De Aloysio, D et al

in Journal of Bone and Mineral Research (2000), 15(8), 1603-14

Phalangeal osteosonogrammetry was introduced as a method for bone tissue investigation in 1992. It is based on the measure of the velocity of ultrasound (amplitude-dependent speed of sound [AD-SoS]) and ... [more ▼]

Phalangeal osteosonogrammetry was introduced as a method for bone tissue investigation in 1992. It is based on the measure of the velocity of ultrasound (amplitude-dependent speed of sound [AD-SoS]) and on the interpretation of the characteristics of the ultrasound signal. In this study we have collected a database of 10,115 subjects to evaluate the performance of AD-SoS and to develop a parameter that is able to quantify the signal characteristics: ultrasound bone profile index (UBPI). The database only includes females of which 4.5% had documented vertebral osteoporotic fractures, 16% lumbar spine dual X-ray absorptiometry (DXA), and 6% hip DXA. The analysis of the ultrasound signal has shown that with aging the UBPI, first wave amplitude (FWA), and signal dynamics (SDy) follow a trend that is different from the one observed for AD-SoS; that is, there is no increase during childhood. In the whole population, the risk of fracture per SD decrease for AD-SOS was odds ratio (OR) 1.71 (CI, 1.58-1.84). The AD-SoS in fractured subjects was significantly lower than in a group of age-matched nonfractured subjects (p < 0.0001). In a small cohort of hip-fractured patients UBPI proved to be lower than in a control age-matched group (p < 0.0001). When the World Health Organization (WHO) working group criteria were applied to this population to identify the T score value for osteoporosis, for AD-SoS we found a T score of -3.2 and for UBPI we found a T score of -3.14. Sixty-six percent of vertebral fractures were below the AD-SoS -3.2 T score and 62% were below UBPI -3.14. We observed the highest incidence of fractures (63.6%) among subjects with AD-SoS who had both DXA T score values below the threshold. We conclude from this study that ultrasound investigation at the hand phalanges is a valid methodology for osteoporosis assessment. It has been possible to quantify signal changes by means of UBPI, a parameter that will improve the possibility of investigating bone structure. [less ▲]

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See detailDes indicateurs européens de qualité de l'éducation : les langues étrangères : un premier exercice
Blondin, Christiane ULg; Demeuse, Marc ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2000), (3-4), 161-169

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See detailSurfactin and iturin A effects on Bacillus subtilis surface hydrophobicity.
Ahimou, F.; Jacques, P.; Deleu, Magali ULg

in Enzyme & Microbial Technology (2000), 27(10), 749-754

The synthesis of extracellular molecules such as biosurfactants should have major consequences on bacterial adhesion. These molecules may be adsorbed on surfaces and modify their hydrophobicities. Certain ... [more ▼]

The synthesis of extracellular molecules such as biosurfactants should have major consequences on bacterial adhesion. These molecules may be adsorbed on surfaces and modify their hydrophobicities. Certain strains of Bacillus subtilis synthesize the lipopeptides, which exhibit antibiotic and surface active properties. In this study the high-performance liquid chromatography (HPLC) analysis of the culture supernatants of the seven B. subtilis strains, showed that the lipopeptide profile varied greatly according to the strain. Among the three lipopeptide types, only iturin A was produced by all B. subtilis strains. Bacterial hydrophobicity, evaluated by the water contact angle measurements and the hydrophobic interaction chromatography, varied according to the strain. Two strains (ATCC 15476 and ATCC 15811) showing extreme behaviors in term of hydrophobicity were selected to study surfactin and iturin A effects on bacterial hydrophobicity. The two lipopeptides modified the B. subtilis surface hydrophobicity. Their effects varied according to the bacterial surface hydrophobic character, the lipopeptide type and the concentration. Lipopeptide adsorption increased the hydrophobicity of the hydrophilic strain but decreased that of the hydrophobic. Comparison of lipopeptide effects on B. subtilis surface hydrophobicity showed that surfactin was more effective than iturin A for the two strains tested. [less ▲]

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See detailViolence et adolescent, un lien naturel?
Malchair, Alain ULg

in Revue des Hôpitaux de Jour Psychiatriques et des Thérapies Institutionnelles (2000), (2), 23-26

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See detailInfluence of ventilatory pattern on respiratory impedance measured by impulse oscillometry in horses
Van Erck, Emmanuelle ULg; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Pflügers Archiv : European Journal of Physiology (2000), 440(R219-R220),

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See detailComparison of a nurse-directed weight-based heparin nomogram with a standard doctor-based regimen
FRAIPONT, V; LAMBERMONT, Bernard ULg; MOONEN, M et al

in Intensive Care Medicine (2000), 26(4), 218

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See detailComparative Effects Of Deltamethrin And Neem Kernel Solution Treatments On Diamondback Moth And Cotesia Plutellae (Hym., Braconidae) Parasitoid Populations In The Cotonou Peri-Urban Area In Benin
Goudegnon, Ae.; Kirk, Aa.; Schiffers, Bruno ULg et al

in Journal of Applied Entomology-Zeitschrift Fur Angewandte Entomologie (2000), 124(3-4), 141-144

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See detailEffect of gonadal steroids on pituitary LH secretion and mediobasal hypothalamic GnRH mRNA in ferrets.
Bakker, Julie ULg; Baum, M. J.

in Journal of Reproduction and Fertility (2000), 119(2), 315-21

In vitro release and content of GnRH in mediobasal hypothalamic slices are reduced by ovariectomy of female ferrets but are not affected by castration of male ferrets in breeding condition. The aim of the ... [more ▼]

In vitro release and content of GnRH in mediobasal hypothalamic slices are reduced by ovariectomy of female ferrets but are not affected by castration of male ferrets in breeding condition. The aim of the present study was to determine whether this sex difference reflects a sexually dimorphic effect of gonadal steroids on mediobasal hypothalamic GnRH mRNA content of male and female ferrets killed 4 weeks after gonadectomy, either with or without steroid hormone replacement. This time interval exceeds the 6-10 days needed for increments in plasma LH concentrations to stabilize after gonadectomy of ferrets of both sexes. In situ hybridization using an (35)S-labelled oligoprobe complementary to the human GnRH coding region showed that the number of mediobasal hypothalamic neurones and the cellular content of GnRH mRNA did not differ significantly among groups of male and female ferrets that were either in breeding condition or that had been gonadectomized and treated with sex steroids or oil vehicle. These results indicate that gonadal hormones regulate mediobasal hypothalamic GnRH biosynthesis and release in both sexes via post-transcriptional events that may include GnRH mRNA translation or the conversion of pre-pro GnRH precursor into mature GnRH. [less ▲]

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See detailSocial Feeding In Ladybird Beetles: Adaptive Significance And Mechanism
Hemptinne, Jl.; Gaudin, M.; Dixon, Afg. et al

in Chemoecology (2000), 10(3), 149-152

Young larvae of Adalia bipunctata search an area more intensively when exposed to the odour from other larvae feeding on aphids than when exposed only to the odour of aphids. In an olfactometer young ... [more ▼]

Young larvae of Adalia bipunctata search an area more intensively when exposed to the odour from other larvae feeding on aphids than when exposed only to the odour of aphids. In an olfactometer young larvae were significantly attracted either to the odour of crushed aphids or larvae feeding on aphids, but not to that of aphids, larvae, larvae plus aphids or larvae feeding on an artificial diet. That is, the change in searching behaviour appears in response to a volatile released by aphids when attacked. The odour released by crushed aphids is made up entirely of aphid alarm pheromone, b-farnesene. It is likely that the adaptive significance of this response is that it increases the ability of larvae to locate larvae that have already caught prey. By sharing the aphid kill of another larva it is likely that a first instar ladybird larva greatly increases its probability of surviving to the next instar. It is suggested that this social feeding is facilitated by egg clustering, which also may additionally account for why aphidophagous ladybirds lay their eggs in clusters. [less ▲]

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See detailDependency of Premature Ventricular Contractions on Heart Rate and Circadian Rhythms During Childhood
Massin, M. M.; Maeyns, K.; Withofs, Nadia ULg et al

in Cardiology (2000), 93(1-2), 70-3

The aim of the present study was to identify and quantify the rate dependence of premature ventricular contractions (PVC) during childhood. A 24-hour Holter recording was performed in 16 consecutive ... [more ▼]

The aim of the present study was to identify and quantify the rate dependence of premature ventricular contractions (PVC) during childhood. A 24-hour Holter recording was performed in 16 consecutive children, aged 22 days to 11 years (mean age 5.6 years), with frequent (>5,000/day), isolated monomorphic PVC. Those PVC were identified and the length of the preceding sinus cycle was measured. The values were ordered into 50-ms class intervals, and the percentage of PVC for each class was calculated and then analyzed by linear regression analysis. On the basis of the significance of the p value, and the positive or negative value of the slope, we identified a tachycardia-enhanced, a bradycardia-enhanced, and an indifferent pattern. Chronobiologic analysis was made by the cosinor method. All the patients had upper and lower limits of cycle length beyond which PVC disappeared. A tachycardia-enhanced pattern was present in 7 patients and an indifferent one in 9 patients. In the latter a second-degree polynomial correlation was systematically found. Children but not infants had a significant circadian variation in the frequency of PVC with a very variable time of highest incidence. In conclusion, it is possible to identify a circadian rhythm of PVC and a spontaneous trend between their incidence and the length of the preceding cardiac cycle in children. [less ▲]

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See detailNeuroendocrine regulation of GnRH release in induced ovulators.
Bakker, Julie ULg; Baum, M. J.

in Frontiers in Neuroendocrinology (2000), 21(3), 220-62

GnRH is the key neuropeptide controlling reproductive function in all vertebrate species. Two different neuroendocrine mechanisms have evolved among female mammals to regulate the mediobasal hypothalamic ... [more ▼]

GnRH is the key neuropeptide controlling reproductive function in all vertebrate species. Two different neuroendocrine mechanisms have evolved among female mammals to regulate the mediobasal hypothalamic (MBH) release of GnRH leading to the preovulatory secretion of LH by the anterior pituitary gland. In females of spontaneously ovulating species, including rats, mice, guinea pigs, sheep, monkeys, and women, ovarian steroids secreted by maturing ovarian follicles induce a pulsatile pattern of GnRH release in the median eminence that, in turn, stimulates a preovulatory LH surge. In females of induced ovulating species, including rabbits, ferrets, cats, and camels, the preovulatory release of GnRH, and the resultant preovulatory LH surge, is induced by the receipt of genital somatosensory stimuli during mating. Induced ovulators generally do not show "spontaneous" steroid-induced LH surges during their reproductive cycles, suggesting that the positive feedback actions of steroid hormones on GnRH release are reduced or absent in these species. By contrast, mating-induced preovulatory surges occasionally occur in some spontaneously ovulating species. Most research in the field of GnRH neurobiology has been performed using spontaneous ovulators including rat, guinea pig, sheep, and rhesus monkey. This review summarizes the literature concerning the neuroendocrine mechanisms controlling GnRH biosynthesis and release in females of several induced ovulating species, and whenever possible it contrasts the results with those obtained for spontaneously ovulating species. It also considers the adaptive, evolutionary benefits and disadvantages of each type of ovulatory control mechanism. In females of induced ovulating species estradiol acts in the brain to induce aspects of proceptive and receptive sexual behavior. The primary mechanism involved in the preovulatory release of GnRH among induced ovulators involves the activation of midbrain and brainstem noradrenergic neurons in response to genital-somatosensory signals generated by receipt of an intromission from a male during mating. These noradrenergic neurons project to the MBH and, when activated, promote the release of GnRH from nerve terminals in the median eminence. In contrast to spontaneous ovulators, there is little evidence that endogenous opioid peptides normally inhibit MBH GnRH release among induced ovulators. Instead, the neural signals that induce a preovulatory LH surge in these species seem to be primarily excitatory. A complete understanding of the neuroendocrine control of ovulation will only be achieved in the future by comparative studies of several animal model systems in which mating-induced as well as spontaneous, hormonally stimulated activation of GnRH neurons drives the preovulatory LH surge. [less ▲]

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See detailCrucial role of the amino-terminal tyrosine residue 42 and the carboxy-terminal PEST domain of IkappaBalpha in NF-kappaB activation by an oxidative stress
Schoonbroodt, Sonia; Ferreira, V.; Best-Belpomme, Martin et al

in Journal of Immunology (2000)

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See detailGenetic Determinants Of Bovine Leukemia Virus Pathogenesis
Willems, Luc ULg; Burny, A.; Collete, Delphine et al

in Aids Research and Human Retroviruses (2000), 16(16), 1787-95

The understanding of HTLV-induced disease is hampered by the lack of a suitable animal model allowing the study of both viral replication and leukemogenesis in vivo. Although valuable information has been ... [more ▼]

The understanding of HTLV-induced disease is hampered by the lack of a suitable animal model allowing the study of both viral replication and leukemogenesis in vivo. Although valuable information has been obtained in different species, such as rabbits, mice, rats, and monkeys, none of these systems was able to conciliate topics as different as viral infectivity, propagation within the host, and generation of leukemic cells. An alternate strategy is based on the understanding of diseases induced by viruses closely related to HTLV-1, like bovine leukemia virus (BLV). Both viruses indeed belong to the same subfamily of retroviruses, harbor a similar genomic organization, and infect and transform cells of the hematopoietic system. The main advantage of the BLV system is that it allows direct experimentation in two different species, cattle and sheep. [less ▲]

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See detailLes populations de grands mammifères et d'autruches du Parc National de Zakouma au Tchad : statuts et tendances évolutives
Dejace, Philippe; Gauthier, Laure; Bouché, Philippe ULg

in Revue d'Ecologie (2000), 55

Zakouma National Park is situated in the south-east of Chad Republic and covers more than 3 000 km2. Rehabilitated in 1989, it is certainly one of the best-preserved wildlife sanctuaries in Sahelian ... [more ▼]

Zakouma National Park is situated in the south-east of Chad Republic and covers more than 3 000 km2. Rehabilitated in 1989, it is certainly one of the best-preserved wildlife sanctuaries in Sahelian Africa. Aerial census shows that most of its original fauna is still present, making the Park an exception in the region despite the recent troubled history of this country. Total population estimates of elephants, buffaloes and topis are given and distribution maps are presented for eleven species. Results are compared with two previous estimates. Except for the ostrich and perhaps the warthog, probably in regression, the populations of two species are stable (hartebeest and giraffe) while the others are clearly increasing (buffalo, topi, elephant and roan antelope). Those encouraging results are the consequence of the remoteness of the park, wich is far from any big town, and also overflooded and isolated during the wet season. Those facts contribute to maintain the human pressure at a sustainable level. [less ▲]

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See detailRural Labor Flows in China
Florence, Eric ULg

in Revue Bibliographique de Sinologie = Review of Bibliography in Sinology (2000), Vol. XVIII

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See detailOn a q-analogue of the spin-orbit coupling
Micu, M.; Stancu, Floarea ULg

in Journal of Physics : A Mathematical & General (2000), A33

Based on the tensor method, a $q$-analogue of the spin-orbit coupling is introduced in a $q$-deformed Schr\" odinger equation, previously derived for a central potential. Analytic expressions for the ... [more ▼]

Based on the tensor method, a $q$-analogue of the spin-orbit coupling is introduced in a $q$-deformed Schr\" odinger equation, previously derived for a central potential. Analytic expressions for the matrix elements of the representations $j = \ell \pm 1/2$ are derived. The spectra of the harmonic oscillator and the Coulomb potential are calculated numerically as a function of the deformation parameter, without and with the spin-orbit coupling. The harmonic oscillator spectrum presents strong analogies with the bound spectrum of an Woods-Saxon potential customarily used in nuclear physics. The Coulomb spectrum simulates relativistic effects. The addition of the spin-orbit coupling reinforces this picture. [less ▲]

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See detailEffects of glucocorticoids on hepatic sensitivity to insulin and glucagon in man.
Dirlewanger, M.; Schneiter, P. H.; Paquot, Nicolas ULg et al

in Clinical Nutrition (2000), 19(1), 29-34

AIMS: This study was undertaken to determine the effects of a short-term dexamethasone treatment on hepatic sensitivities to insulin and glucagon. METHODS: Eleven healthy subjects were studied during one ... [more ▼]

AIMS: This study was undertaken to determine the effects of a short-term dexamethasone treatment on hepatic sensitivities to insulin and glucagon. METHODS: Eleven healthy subjects were studied during one or several of four protocols. In all protocols, somatostatin was infused continuously to inhibit pancreatic hormone secretion. In protocol 1, basal insulin was infused over 300 min while glucagon was infused at a rate of 0.5 mg/kg(-1)/min(-1)during 180 min, then at a rate of 1.5 ng/kg(-1)/min(-1)during 150 min. In protocol 2, the same experiment was performed after a 2 day treatment with 8 mg/day dexamethasone. In protocol 3, the two-step glucagon infusion was performed during insulin infusion at a rate aimed to reproduce the hyperinsulinemia observed during protocol 2. In protocol 4, continuous basal insulin and low glucagon (0.5 mg/kg(-1)/min(-1)) were infused over 330 min. RESULTS: In protocol 1, plasma glucose rose transiently by 2.0 +/- 0.3 mmol/l when the glucagon rate was increased and glucose production increased by 1.4 +/- 0.5 micromol/kg(-1)/min(-1). In protocol 2, the insulin infusion rate (1.85 +/- 0.36 nmol/kg(-1)/min(-1)) required to maintain glycemia was 3.3-fold higher than during protocol 1. Glucagon-induced stimulation of glycemia (by 1.47 +/- 0.5 mmol/l) and endogenous glucose production (by 0.8 +/- 0.3 micromol/kg(-1)/min(-1)) were blunted, but not abolished. In protocol 3, endogenous glucose production was suppressed by 75% by hyperinsulinemia and was not stimulated when the glucagon infusion rate was increased. In protocol 4, endogenous glucose production did not change significantly with time. CONCLUSION: These results indicate that high dose glucocorticoids induce a marked hepatic insulin resistance. Stimulation of glucose production by hyperglucagonemia was maintained in spite of hyperinsulinemia which can be attributed to either hepatic insulin resistance and/or increased hepatic glucagon sensitivity. [less ▲]

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See detailVariability of Doppler ultrasound measurements of hindlimb blood flow in conscious horses
Raisis, Anthea L; Young, L. E.; Meire, H. B. et al

in Equine Veterinary Journal (2000), 32(2), 125-132

Ultrasonography of the left and right femoral artery and vein was performed in 5 conscious horses. Velocity waveforms, recorded using low pulse repetition frequency, were used to calculate time-averaged ... [more ▼]

Ultrasonography of the left and right femoral artery and vein was performed in 5 conscious horses. Velocity waveforms, recorded using low pulse repetition frequency, were used to calculate time-averaged mean velocity (TAV), velocity of component a (TaVa), velocity of component b (TaVb) and volumetric flow. Waveform analysis included calculation of early diastolic deceleration slope (EDDS) and pulsatility index (PI) and subjective description of the waveform. Measurements were recorded at monthly intervals for 6 months. The repeated measurements were used to determine the within-horse variation. Horses were then anaesthetised with halothane and the same measurements recorded 60 min after induction. Differences between measurements recorded in conscious and anaesthetised horses were determined. Within-horse variability for diameters of femoral arteries and veins was small (CV<10%). Within variability for all other measurements was marked (CV>11%), with within-horse variability being the largest for femoral venous flow (CV>35%). Source of variability was measurement error and biological variation. Despite variability in conscious horses it was possible to detect changes in blood flow during anaesthesia. In the femoral arteries, volumetric flow was lower and EDDS and PI was higher in anaesthetised horses. In the femoral veins, volumetric flow was also lower in anaesthetised horses. Therefore Doppler ultrasound appears to be a useful technique for studying blood flow in conscious and anaesthetised horses [less ▲]

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See detailMise au point relative à la croissance folliculaire chez la vache.1. Aspects morphologiques et cinétiques
Hanzen, Christian ULg; Lourtie, O.; Derkenne, F. et al

in Annales de Médecine Vétérinaire (2000), 144

La mise en place du potentiel folliculaire se fait bien avant la naissance chez les mammifères. Le nombre maximal de cellules gamétiques contenues dans l'ovaire est atteint après une première phase de ... [more ▼]

La mise en place du potentiel folliculaire se fait bien avant la naissance chez les mammifères. Le nombre maximal de cellules gamétiques contenues dans l'ovaire est atteint après une première phase de multiplication aux environs du centième jour de gestation chez la vache et décroît progressivement ensuite jusqu'à la naissance. A cette première phase succède une phase de croissance et une phase de maturation, cette dernière concernant surtout l'ovocyte. Cette évolution ne concerne qu'un nombre réduit de follicules, la plupart d'entre eux subissant l'atrésie. La croissance folliculaire présente deux caractéristiques. Elle se manifeste sous la forme de croissances et de régressions successives de plusieurs follicules appelées vagues. Chacune d'entre elles consiste en l'émergence, tous les 7 à 9 jours environ, de plusieurs follicules de diamètre égal ou supérieur à 5 mm parmi lesquels apparaîtra le follicule dominant. Ces vagues ont été décrites au cours du cycle, pendant la puberté, le postpartum et la gestation. Par ailleurs, il est bien connu que parmi les follicules recrutés, c'est à dire dont la croissance s'est poursuivie au-delà d'une taille à laquelle ils subissent habituellement l'atrésie, un nombre limité, caractéristique de l'espèce, va être sélectionné pour arriver éventuellement au stade ovulatoire. Ceux-ci vont à ce moment exercer une dominance tout à la fois fonctionnelle et morphologique sur les autres follicules. [less ▲]

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See detailL’internationalisation des entreprises de distribution. Quelques observations au départ du cas européen
Merenne-Schoumaker, Bernadette ULg

in Géographies. Bulletin de l'Association de Géographes Français (2000), (2000-1), 66-73

To understand the internationalization of retailing, we must understand the internationalization of its entreprises. With this aim in view, one first case is chosen - Europa - and the processus is learned ... [more ▼]

To understand the internationalization of retailing, we must understand the internationalization of its entreprises. With this aim in view, one first case is chosen - Europa - and the processus is learned through the main questions of geography : what where ? which evolution ? who ? how ? why and why there and not elsewhere ? The lead of territory in the strategies and the choices of the firms is clearly showed; this fact confirms the geographical relevance of the thema « Globalization of Retailing ». [less ▲]

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See detailIdentification de cultivars de palmier dattier (Phoenix dactylifera L.) par l'amplification aléatoire d'ADN (RAPD)
Ben Abdallah, Abdallah; Stiti, Kadija; Du Jardin, Patrick ULg et al

in Cahiers Agricultures (2000), 9(2), 103-107

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See detailAssessment of postoperative nausea using a visual analogue scale.
Boogaerts, J G; Vanacker, E; Seidel, Laurence ULg et al

in Acta Anaesthesiologica Scandinavica (2000), 44(4), 470-4

BACKGROUND: Assessment of postoperative nausea intensity is difficult because nausea is a subjective and unpleasant sensation. We propose using the Visual Analogue Scale (VAS) device to increase the ... [more ▼]

BACKGROUND: Assessment of postoperative nausea intensity is difficult because nausea is a subjective and unpleasant sensation. We propose using the Visual Analogue Scale (VAS) device to increase the efficiency and precision in the assessment of nausea. We carried out a pilot study on postoperative patients suffering from nausea to measure the degree of agreement between the VAS scores and those given on a 4-point verbal descriptive scale (VDS). METHODS: Postoperative nausea was evaluated by means of a classical VAS (0-10 cm) device and a 4-point VDS (0=no nausea, 1=mild, 2=moderate, 3=severe) in 128 surgical spontaneously complaining patients. Evaluation was repeated 45 min after rescue medication given if nausea was intractable, lasted more than 10 min or at the request of the patient. Ordinal logistic regression was used to measure the association between VAS and VDS and to determine cut-off points on the VAS. RESULTS: The VAS device was easily understood and used by patients. VAS scores decreased significantly from 5.5+/-2.3 to 1.4+/-1.8 after rescue medication (P=0.002). Application of ordinal logistic regression to pre- and post-medication data combined yielded an agreement of 86% between VAS and VDS and the cut-off points on the VAS were estimated as follows: 0-1 (no nausea), 1+/-4 (mild), 4+/-7 (moderate) and 7+/-10 (severe). CONCLUSION: The VAS method proved to be useful for assessing quantitative nausea intensity and for testing the efficacy of rescue medication. It was found that a cut-off value of 4 on the VAS may be considered as a critical threshold triggering anaesthesiologists or nurses to administer rescue medication. [less ▲]

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See detailNote sous Bruxelles, 23 septembre 1999
Thirion, Nicolas ULg

in Droit des Affaires : Revue Internationale du Droit des Affaires = Ondernemingsrecht : Internationaal Tijdschrift voor Ondernemingsrecht [= DA OR] (2000), 55

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See detailPost-stroke reorganization of hand motor area: a 1 year longitudinal study with focal transcranial magnetic stimulation.
DELVAUX, Valérie ULg; ALAGONA G.; DE PASQUA, Victor ULg et al

in Clinical Neurophysiology (2000), 111(Suppl. 1), 12517-06

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See detailMieux connaître la forêt privée. Appel aux propriétaires.
Rondeux, Jacques ULg; Bourland, Nils ULg

in Silva Belgica (2000), 107(1), 28-29

Dans le cadre de l'appui scientifique à l'Inventaire permanent des Ressources ligneuses de Wallonie, l'Unité de Gestion et Economie forestières (Prof. J. RONDEUX) de la Faculté universitaire des Sciences ... [more ▼]

Dans le cadre de l'appui scientifique à l'Inventaire permanent des Ressources ligneuses de Wallonie, l'Unité de Gestion et Economie forestières (Prof. J. RONDEUX) de la Faculté universitaire des Sciences agronomiques de Gembloux (FUSAGx) a été chargée de compléter l'information générale relative à la forêt privée, en particulier pour mieux identifier son degré de morcellement et l'évolution attendue de celui-ci dans un avenir proche. Ce travail est réalisé en étroite collaboration avec la Cellule Inventaire des Ressources ligneuses Wallonnes (Ir. H. LECOMTE) et bénéficie du soutien de la Société Royale Forestière de Belgique. [less ▲]

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See detailComparison of rectal and infrared ear temperatures in older hospital inpatients.
Smitz, Simon ULg; Giagoultsis, T; Dewé, Walthère ULg et al

in Journal of the American Geriatrics Society (2000), 48(1), 63-6

OBJECTIVES: To assess the agreement between infrared emission detection (IRED) ear and rectal temperatures and to determine the validity of IRED ear thermometry in detecting rectal fever. DESIGN ... [more ▼]

OBJECTIVES: To assess the agreement between infrared emission detection (IRED) ear and rectal temperatures and to determine the validity of IRED ear thermometry in detecting rectal fever. DESIGN: Prospective, convenience sample, unblinded study. SETTING: An acute geriatric unit (teaching hospital) and a multidisciplinary intensive care unit. PARTICIPANTS: The study included 45 inpatients (26 women and 19 men), aged 78.3+/-6.9 years, admitted over a 4-month period. Twelve of the patients were definitely infected. MEASUREMENTS: Sequential rectal (RT) and ear temperature (ET) measurements were performed using mercury-in-glass and IRED ear thermometers, respectively. IRED ear temperatures were measured at both ears (unadjusted mode), with the highest of six ear temperatures considered the true value. RESULTS: Mean RT (37.39 degrees C +/- 0.52 degrees C) was significantly (P<.001) higher than mean ET (36.89 degrees C +/-0.59 degrees C). A highly significant positive correlation was found between RT and ET (slope = 0.69; 95% CI, 0.52-0.86; P<.001; r = 0.78). The mean bias (mean of the differences) between RT and ET was 0.50 degrees C +/-0.37 degrees C (95% CI, 0.41 degrees C-0.59 degrees C), and the 95% limits of agreement -0.22 degrees C and 1.23 degrees C (95% CI, -0.38 degrees C to 1.39 degrees C). According to the standard criterion (RT > or =37.6 degrees C), 14 patients were febrile. Using an optimum IRED ear fever threshold (37.2 degrees C), the sensitivity and specificity of IRED ear thermometry for predicting rectal fever were 86% and 89%, respectively (positive predictive value, 80%; negative predictive value, 93%). CONCLUSIONS: The degree of agreement between rectal temperature and the highest of six IRED ear temperatures was acceptable. Using an optimal IRED ear fever threshold of 37.2 degrees C (99 degrees F), IRED ear thermometry had acceptable sensitivity and specificity for predicting rectal fever. [less ▲]

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See detailSynthesis of new hydrophilic γ-substituted poly(ε-caprolactone)s
Lecomte, Philippe ULg; D'aloia, Violetta; Mazza, Michaël et al

in Polymer Preprints (2000), 41(2), 1534-1535

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See detail2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: evaluation of the spin trapping properties
Olive, Gilles ULg; Mercier, Anne; Le Moigne, François et al

in Free Radical Biology & Medicine (2000), 28(3), 403-408

The 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-l-oxide (EMPO), an easily prepared pyrroline-Noxide has been tested as a free radical scavenger. Spin adducts of superoxide, hydroxyl radical, and ... [more ▼]

The 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-l-oxide (EMPO), an easily prepared pyrroline-Noxide has been tested as a free radical scavenger. Spin adducts of superoxide, hydroxyl radical, and other free radicals were characterized in phosphate buffer at pH 7.0 and 5.6. At pH 7 in phosphate buffer, the EMPO/O2 2• spin adduct was estimated to be about five times more persistent than its DMPO analogue. Furthermore, its decay does not produce the EMPO/HO• adduct. [less ▲]

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See detailCore-polarization effects in the Cadmium Isoelectronic Sequence
Biémont, Emile ULg; Froese Fischer, C.; Godefroid, M. R. et al

in Physical Review. A (2000), 62

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See detailRe-entrant Peierls distortion in IV-VI compounds
Raty, Jean-Yves ULg; Gaspard, Jean-Pierre ULg; Bichara, C. et al

in Physica B: Condensed Matter (2000), 276-278

At room temperature, the local structure of crystalline group V elements (As, Sb,middotmiddotmiddot) and their IV-VI isoelectronic compounds (GeSe,middotmiddotmiddot) is governed by a Peierls distortion ... [more ▼]

At room temperature, the local structure of crystalline group V elements (As, Sb,middotmiddotmiddot) and their IV-VI isoelectronic compounds (GeSe,middotmiddotmiddot) is governed by a Peierls distortion of the simple cubic or NaCl structure which is a symmetry breaking electronic instability. The morphology of the distortion is determined by the filling ratio of the p-band; for a half-filled p-band, the sixfold coordination becomes 3 (short, covalent) +3 (long, van der Waals). In general, at high temperature, the structure recovers its higher coordination number. Neutron diffraction experiments have been made in the liquid state at lambda=0.7 Aring. It is observed that the Peierls distortion is still present in the liquid for most IV-VI compounds. This behavior is observed and discussed for a series of Sn and Ge chalcogenides: SnS, SnSe, GeS, GeSe and GeTe and their temperature evolution is discussed. GeSe and GeTe show an interesting re-entrant phase behavior. The heaviest IV-VI compound SnTe does not show a distorted state. We demonstrate that the hardness of the repulsive potential is a key parameter in this mechanism [less ▲]

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See detailPrivatisation d'entreprises publiques, économie de marché et transformation des systèmes juridiques étatiques
Thirion, Nicolas ULg

in Pyramides : Revue du Laboratoire d’Etudes et de Recherches en Administration Publique (2000), (2), 85-110

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See detailRespiration As The Main Determinant Of Carbon Balance In European Forests
Valentini, R.; Matteucci, G.; Dolman, Aj. et al

in Nature (2000), 404(6780),

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See detailEffect of grade on disease-free survival and overall survival in FIGO Stage I adenocarcinoma of the endometrium.
Delaloye, J-F; Pampallona, S; COUCKE, Philippe ULg et al

in European Journal of Obstetrics & Gynecology & Reproductive Biology (2000), 88

Abstract Objective : To analyse the effect of differentiation on disease-free survival (DFS) and overall survival (OS) in patients with stage I adenocarcinoma of the endometrium. Patients and methods ... [more ▼]

Abstract Objective : To analyse the effect of differentiation on disease-free survival (DFS) and overall survival (OS) in patients with stage I adenocarcinoma of the endometrium. Patients and methods : From 1979 to 1995, 350 patients with FIGO stage IA–IC with well (G1), moderately (G2) or poorly (G3) differentiated tumors were treated with surgery and high dose-rate brachytherapy with or without external radiation. Median age was 65 years (39–86 years). Results : The 5-year DFS was 8863% for the G1 tumors, 7764% for the G2 tumors, and 6767% for the G3 tumors (P50.0049). With regard to the events contributing to DFS, the 5-year cumulative percentage of local relapse was 4.6% for the G1 tumors, 9.0% for the G2 tumors, and 4.6% (P50.027) for the G3 tumors. Cumulative percentage of metastasis was 1.4, 6.3 and 7.2% (P,0.001), respectively, whereas percentages of death were 6.0, 7.9 and 20.7% (P,0.001). The 5-year OS was 9163, 8364 and 7667%, respectively (P50.0018). In terms of multivariate hazard ratios (HR), the relative differences between the three differentiation groups correspond to an increase of 77% of the risk of occurrence of either of the three events considered for the DFS (HR51.77, 95% Cl [0.94–3.33]), (P50.078) for the G2 tumors and of 163% (HR52.63, 95% Cl [1.27–5.43]), (P50.009) for the G3 tumors with respect to the G1 tumors. The estimated relative hazards for OS are, respectively, in line with those for DFS: HR51.51 (P50.282) for the G2 tumors; and HR53.37 (P50.003) for the G3 tumors. Conclusion : Patients with grade 1 tumors are those least exposed to either local relapse, metastasis, or death. In contrast patients with grade 2 tumors seem to be at higher risk of metastasis, whereas patients with grade 3 tumors appear at higher risk of death. Since we have looked at the first of three competing events (local relapse, metastasis and death), this suggests that patients with grade 3 tumors probably progress to death so fast that local relapse, if any, cannot be observed. Ó 2000 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailLocalization of Nadph-Protochlorophyllide Reductase in Plastids of Barley at Different Greening Stages
Barthelemy, X.; Bouvier, G.; Radunz, A. et al

in Photosynthesis Research (2000), 64(1), 63-76

The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley ... [more ▼]

The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley seedlings. Pchlide pigment and POR protein contents were evaluated in different plastid membrane fractions by fluorescence spectroscopy and immunoblot analysis using a monospecific polyclonal antibody raised against the purified enzyme. Fluorescence analysis showed the presence of Pchlide in both the envelope and thylakoid membranes. During greening, the Pchlide content, expressed on a total protein basis, decreased in thylakoid membranes, whereas it increased in the envelope membranes. POR proteins were detected mainly in thylakoid membranes at early greening stages. In contrast, the weak amount of POR proteins was associated more specifically with envelope membranes of mature chloroplasts. Whatever the greening stage, thylakoid-bound Pchlide and POR proteins were more abundant in the thylakoid regions which remained unsolubilized after mild Triton treatment used as standard procedure to prepare PS II particles. This suggests the preferential association of Pchlide and POR to the appressed regions of thylakoids. [less ▲]

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See detailGenetic Knowledge and Social Marginalization in the People's Republic of China
Florence, Eric ULg

in Revue Bibliographique de Sinologie = Review of Bibliography in Sinology (2000), Vol. XVII

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See detailAcute pancreatitis attributed to the use of interferon alfa-2b
Eland, I. A.; Rasch, M. C.; Sturkenboom, M. J. et al

in Gastroenterology (2000), 119(1), 230-233

Two patients experienced episodes of acute pancreatitis shortly after starting treatment with interferon alfa-2b (IFN-alpha) for a chronic hepatitis C infection. The first patient was a 40-year-old man ... [more ▼]

Two patients experienced episodes of acute pancreatitis shortly after starting treatment with interferon alfa-2b (IFN-alpha) for a chronic hepatitis C infection. The first patient was a 40-year-old man who developed acute pancreatitis after 15 weeks of treatment with 3 MU IFN-alpha subcutaneously (SC) 3 times weekly and 1200 mg ribavirin. After disappearance of symptoms and normalization of laboratory values, oral intake of solid foods and IFN-alpha therapy were restarted. Within hours, a relapse of acute pancreatitis occurred. A rechallenge with IFN-alpha 4 days later was followed by a prompt increase in serum lipase level, and IFN-alpha therapy was discontinued. The second patient was a 38-year-old man who developed acute pancreatitis 2 hours after SC administration of 5 MU IFN-alpha. Ultrasound endoscopy showed sludge in the gallbladder. The patient was rechallenged 5 weeks later with 3 MU IFN-alpha SC. Although serum amylase and lipase levels increased after readministration of IFN-alpha, treatment was continued. The patient was readmitted 2 weeks later with severe abdominal pain, and IFN-alpha administration was discontinued. Considering the temporal relationship between the start of IFN-alpha treatment and development of acute pancreatitis, the absence of other clear etiologic factors for acute pancreatitis, disappearance of symptoms after discontinuation of IFN-alpha, and positive reactions to rechallenge, IFN-alpha is the most probable cause for development of acute pancreatitis in these patients. [less ▲]

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See detailBeta-radiation for coronary in-stent restenosis
Latchem, D-R; Urban, P; Goy, JJ et al

in Catheterization and Cardiovascular Interventions (2000), 51(4), 422-429

To determine the feasibility and safety of an intracoronary beta-radiation device in preventing the recurrence of in-stent restenosis (ISR) after successful angioplasty, we studied 37 patients treated ... [more ▼]

To determine the feasibility and safety of an intracoronary beta-radiation device in preventing the recurrence of in-stent restenosis (ISR) after successful angioplasty, we studied 37 patients treated with beta-radiation (30-mm strontium-90 source) after angioplasty. The mean reference diameter was 2.9 ± 0.5 mm, and 62% of lesions were diffuse, including four total occlusions. Beta-radiation was successfully delivered in 36 of 37 (97%) cases. Over the course of 7.1 ± 4.5 mo follow-up, there were no myocardial infarctions and three deaths: one from preexisting malignancy, one from progressive cardiac failure, and one from sudden cardiac death. Target vessel revascularization (TVR) was performed in seven of 36 (19%) patients. Thirty patients underwent angiography at 6 mo; three (10%) experienced restenosis (diameter stenosis > 50%) at the target site, four (13%) had edge stenoses, and two (7%) had late (> 1 mo) thrombotic occlusions. Beta-radiation for ISR is associated with encouragingly low rates of target lesion restenosis and TVR. Further improvements are needed to solve the limitations of the edge effect and late occlusion. [less ▲]

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See detailEcological, morphological and allozymic differentiation between diploid and tetraploid knapweeds (Centaurea jacea) from a contact zone in the Belgian Ardennes
Hardy, O. J.; Vanderhoeven, SONIA ULg; De Loose, M. et al

in New Phytologist (2000), 146(2), 281-290

In the northeastern part of Belgium, the Centaurea jacea complex shows extensive morphological variation and is represented by a diploid (2n = 22) and a tetraploid (2n = 44) cytotype. Polysomic ... [more ▼]

In the northeastern part of Belgium, the Centaurea jacea complex shows extensive morphological variation and is represented by a diploid (2n = 22) and a tetraploid (2n = 44) cytotype. Polysomic inheritance of allozyme markers in the tetraploids, suggesting autopolyploidy, is here demonstrated for the first time. In order to test whether the tno cytotypes occupy distinct habitats and possess different gene pools, patterns of allozymic and morphological variation were investigated in relation to ploidy level and site characteristics in 26 populations from the Belgian Ardennes. The two cytotypes showed a parapatric distribution, the diploids occurring at higher elevations (mostly above 500 m) than the tetraploids (mostly below 500 m). Three mixed populations were found near the contact zone of the two cytotypes. Within the mixed populations no triploid plant and no evidence for gene flow between cytotypes were found, despite widely overlapping flowering periods. The two cytotypes can be distinguished on the basis of morphological traits and enzymatic gene pools. The congruence of morphological and allozymic variation with chromosome numbers suggests a secondary contact between the two cytotypes with limited gene flow between them. The origin and persistence of the parapatric distribution are discussed. [less ▲]

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See detailLes composantes d'un management efficace dans le cadre des organisations sportives
Cloes, Marc ULg

in Sport (2000), 172

L'objectif de cet article consiste à réaliser une synthèse des notions abordées par les auteurs du colloque "Initiatives en matière de management du sport" et de fournir un outil permettant aux ... [more ▼]

L'objectif de cet article consiste à réaliser une synthèse des notions abordées par les auteurs du colloque "Initiatives en matière de management du sport" et de fournir un outil permettant aux responsables d'organisatons sportives de s'engager dans une auto-analyse des structures dont ils ont la charge. Pour rappel, le management se définit comme l'action de gérer, de diriger, d'assurer le succès d'une organisation. [less ▲]

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See detailEffet de l’ammoniac sur la fibre C impliquée dans le réflexe de la toux chez le porc
Moreaux, B.; Nemmar, R.; Gustin, Pascal ULg

in Journées de la Recherche Porcine en France (2000), 32

The effect of ammonia on the C-fibre which is involved in the citric acid induced coughing reflex was assessed. The piglets were exposed during a period of 24 hours or 4 days to a concentration of 15 or ... [more ▼]

The effect of ammonia on the C-fibre which is involved in the citric acid induced coughing reflex was assessed. The piglets were exposed during a period of 24 hours or 4 days to a concentration of 15 or 30 ppm ammonia in an inhalation chamber . During this challenge, the animais underwent cough induction tests at two days intervals. Another group was exposed to ammonia (30 ppm) for 48 hours, after this period, substance P was assayed in liquids collected from broncho-alvealor and tracheal washings (BAL and TAL) and in tracheal and airway mucosa . Ammonia (30 ppm) induced an inhibition 0f the coughing reflex in pigs. The minimum period of exposure necessary to provoke this effect was 48 hours. The same ammonia concentration aiso induced o significant increase in substance P concentrations in BAL and TAL and a significant decrease in concentrations of the tracheal mucosa. We conclude that ammonia at o concentration of 30 ppm inhibits the citric acid induced coughing reflex. The effect was partly mediated by the release of substance P from the C-fibre. [less ▲]

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See detailConsequences of total and subtotal myeloperoxidase deficiency: risk or benefit ?
Kutter, D.; Devaquet, P.; Vanderstocken, G. et al

in Acta Haematologica (2000), 104(1), 10-5

A group of 100 totally or subtotally myeloperoxidase (MPO)-deficient individuals was compared to a reference population of 118 probands selected at random. Data for a protective effect of the deficiency ... [more ▼]

A group of 100 totally or subtotally myeloperoxidase (MPO)-deficient individuals was compared to a reference population of 118 probands selected at random. Data for a protective effect of the deficiency against cardiovascular damage are presented. On the other hand, a significantly higher occurrence of severe infections and chronic inflammatory processes was noted among the deficient patients. An increased incidence of cancer among the MPO-deficient individuals was not demonstrated. [less ▲]

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See detailAuditory processing in the vegetative state.
Laureys, Steven ULg; Faymonville, Marie ULg; Degueldre, Christian ULg et al

in Brain : A Journal of Neurology (2000), 123 ( Pt 8)

H(2)(15)O-PET was used to investigate changes in regional cerebral blood flow in response to auditory stimulation in patients in the vegetative state. Five patients in a vegetative state of hypoxic origin ... [more ▼]

H(2)(15)O-PET was used to investigate changes in regional cerebral blood flow in response to auditory stimulation in patients in the vegetative state. Five patients in a vegetative state of hypoxic origin were compared with 18 age-matched controls. In addition, the cerebral metabolism of these patients and 53 age-matched controls was studied using [(18)F]fluorodeoxyglucose. In control subjects, auditory click stimuli activated bilateral auditory cortices [Brodmann areas (BA) 41 and 42] and the contralateral auditory association cortices (BA 22). In the patients, although resting metabolism was decreased to 61% of normal values, bilateral auditory areas 41 and 42 showed activation as seen in the controls, but the temporoparietal junction cortex (BA 22) failed to be activated. Moreover, the auditory association cortex was functionally disconnected from the posterior parietal association area (BA 40), the anterior cingulate cortex (BA 24) and the hippocampus, as revealed by psychophysiological interaction analysis. Thus, despite altered resting metabolism, the auditory primary cortices were still activated during external stimulation, whereas hierarchically higher-order multi- modal association areas were not. Such a cascade of functional disconnections along the auditory cortical pathways, from the primary auditory areas to multimodal and limbic areas, suggests that the residual cortical processing observed in the vegetative state cannot lead to the integrative processes that are thought to be necessary for the attainment of the normal level of awareness. [less ▲]

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See detailAlternative area-perimeter ratios for measurement of 2-D shape compactness of habitats.
Bogaert, Jan ULg; Rousseau, R; Van Hecke, P et al

in Applied Mathematics and Computation (2000), 111(1), 71-85

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See detailDual Formulations for the Modeling of Thin Electromagnetic Shells using Edge Elements
Geuzaine, Christophe ULg; Dular, Patrick ULg; Legros, Willy ULg

in IEEE Transactions on Magnetics (2000), 36(4), 799-803

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See detailL'entérotoxémie bovine en Belgique. II. Epizootiologie élémentaire et pathologie descriptive.
Manteca, Christophe; Daube, Georges ULg; Jauniaux, Thierry ULg et al

in Annales de Médecine Vétérinaire (2000), 145

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See detailUse of stable isotope to delineate amphipod trophic status in Antarctic food webs
Nyssen, Fabienne ULg

in Polish Archives of Hydrobiology (2000), 47

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See detailViroses tropicales éruptives
Fraiture, A. L.; Nikkels, Arjen ULg; Pierard-Franchimont, Claudine ULg et al

in Revue Médicale de Liège (2000), 55

Eruptive tropical viroses are severe diseases which may prove to be sometimes fatal diseases. They are characterized by erythémato-purpuric cutaneous lesions associated with hyperthermia. Polyarthralgia ... [more ▼]

Eruptive tropical viroses are severe diseases which may prove to be sometimes fatal diseases. They are characterized by erythémato-purpuric cutaneous lesions associated with hyperthermia. Polyarthralgia or severe haemorrhage can also be part of the clinical presentation. Rapid diagnosis and preventive measures are mandatory, particularly when human transmission is possible. [less ▲]

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See detailTransition rates of the resonance line doublet in the Cl I sequence, Ar II to Ge XVI
Biémont, Emile ULg; Träbert, E.

in Journal of Physics : B Atomic Molecular & Optical Physics (2000), 33

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See detailVoxel-based analysis of confounding effects of age and dementia severity on cerebral metabolism in Alzheimer's disease
Salmon, Eric ULg; Collette, Fabienne ULg; Degueldre, Christian ULg et al

in Human brain mapping (2000), 10(1), 39-48

Alzheimer's disease is characterized by early hippocampal lesions, but neuropathological and functional imaging studies have also demonstrated involvement of associative cortices in patients suffering ... [more ▼]

Alzheimer's disease is characterized by early hippocampal lesions, but neuropathological and functional imaging studies have also demonstrated involvement of associative cortices in patients suffering from this illness. New image-processing technologies have led to demonstration of predominant posteromedial cortical metabolic impairment in the disease. Confounding effects of both age and dementia severity on brain metabolism were assessed using categorical and correlational analyses performed with Statistical Parametric Mapping. Posterior cingulate and precuneus metabolism, assessed by positron emission tomography, was significantly correlated with age in a population of 46 patients with probable Alzheimer's disease. Metabolism in posterior cingulate and precuneus was higher in elderly than in younger patients with a diagnosis of Alzheimer's disease, even when dementia severity was taken as a confounding covariate. The data suggest that the sensitivity of positron emission tomography for the diagnosis of Alzheimer's disease is reduced in elderly cases, where less severe pathology is sufficient to induce clinical symptoms of dementia. Conversely, higher posteromedial metabolic impairment in early onset cases may reflect greater density of regional cerebral lesions or major decrease of functional afferences in a richly connected multimodal associative area. Posterior cingulate metabolism was also correlated to dementia severity, even when age was taken as a confounding covariate, whereas metabolism in the hippocampal formation was not shown to correlate with global cognitive deficit. Functional correlation was maintained between posterior cingulate and middle frontal cortex in demented patients as in elderly controls. The key role of posteromedial cortex in cognitive dysfunction assessed in Alzheimer's disease is probably related to its highly integrated position within attentional, visuospatial and memory neuronal networks. [less ▲]

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See detailNew strategy for the validation of chromatographic bioanalytical methods
Chapuzet, E.; Mercier, N.; Bervoas-Martin, S. et al

in STP Pharma Pratiques (2000), 10(1), 21-38

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See detailDifferences in associations of familial and nutritional factors with serum lipids between boys and girls: the Luxembourg Child Study.
Guillaume, Michèle ULg; Lapidus, L.; Lambert, A.

in American Journal of Clinical Nutrition (2000), 72(2), 384-8

BACKGROUND: Sex differences in the effects of genetic and environmental factors on circulating lipids have been examined mainly in adults, in whom the influences of sex steroid hormones are well known ... [more ▼]

BACKGROUND: Sex differences in the effects of genetic and environmental factors on circulating lipids have been examined mainly in adults, in whom the influences of sex steroid hormones are well known. OBJECTIVE: Our objective was to determine the effect of sex on genetic and environmental influences on serum lipids in prepubertal boys and girls. DESIGN: Children aged 6-8, 8-10, and 10-12 y (n = 1028) were selected at random in the Belgian province of Luxembourg, a region in Europe with a high prevalence of risk factors for cardiovascular disease and diabetes. Blood glucose and serum cholesterol, triacylglycerol, and insulin concentrations were measured, and anthropometric data and blood pressure were recorded. Familial data were obtained from standardized questionnaires. Nutritional status was obtained from a 3-d record. Participation was 70.3% of the primary cohort. RESULTS: Cholesterol, triacylglycerol, and insulin values were among the highest recorded in studies of children. In girls, cholesterol correlated positively with the energy density of intake of saturated fat (r = 0.13, P = 0.001), cholesterol (r = 0.11, P = 0.006), and protein (r = 0.12, P = 0.007) and negatively with the ratio of polyunsaturated to saturated fat intake (r = -0.14, P = 0.001) and the energy density of carbohydrate intake (r = -0.11, P = 0.019). In boys, no such relations were found. Triacylglycerol was not significantly related to nutritional factors. Consistent, independent relations were found between reported elevated cholesterol concentrations in the parental and grandparental generation and cholesterol (r = 0.101, P = 0.011) and triacylglycerol (r = 0.09, P = 0.03) in boys. No such associations were found in girls. CONCLUSION: Environmental and genetic factors may have different effects on serum cholesterol in girls and boys. [less ▲]

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See detailExtensions des techniques de reparation valvulaire mitrale: chirurgie de l'endocardite et des tumeurs valvulaires
Radermecker, M. A.; Gach, Olivier ULg; Henrottaux, G. et al

in Revue Médicale de Liège (2000), 55(10), 950-3

Mitral valve repair has demonstrated its superiority over valve replacement. Mitral valve repair constitutes the optimal therapeutic option in case of degenerative dystrophic, rheumatic, congenital or ... [more ▼]

Mitral valve repair has demonstrated its superiority over valve replacement. Mitral valve repair constitutes the optimal therapeutic option in case of degenerative dystrophic, rheumatic, congenital or ischemic mitral valve disease. These surgical techniques can also be successfully and advantageously applied in case of bacterial endocarditis or tumoral valvular disease. We report a case of staphylococcal endocarditis and another case of posterior leaflet fibroelastoma to show the place of conservative mitral surgery in these unusual indications. [less ▲]

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See detailAutour de Stavelot-Malmedy (VIIe-IXe siècles)
George, Philippe ULg

in Publications de la Section Historique de l'Institut Royal Grand-Ducal de Luxembourg (2000)

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See detailIschaemic Colitis Following Aortoiliac Surgery
VAN DAMME, Hendrik ULg; CREEMERS, Etienne ULg; Limet, Raymond ULg

in Acta Chirurgica Belgica (2000), 100(1), 21-7

Ischaemic colitis following aortoiliac surgery is a feared complication. Its frequency varies from 7% after repair of ruptured abdominal aortic aneurysm (AAA) to 0.6% after bypass for aortoiliac occlusive ... [more ▼]

Ischaemic colitis following aortoiliac surgery is a feared complication. Its frequency varies from 7% after repair of ruptured abdominal aortic aneurysm (AAA) to 0.6% after bypass for aortoiliac occlusive disease (AOD). In order to analyse predisposing factors and outcome of ischaemic colitis, the authors reviewed their clinical experience from 1988 to 1998. It concerns 28 cases (16 ruptured AAA, 7 elective AAA, 5 OAD) of clinically evident colonic ischaemia. This means an incidence of 7% after repair of ruptured AAA, 0.6% after elective AAA repair, and 0.8% after bypass for AOD. Transmural necrosis (grade 3) was observed in 21 patients, grade 2 ischaemia in 5 patients, and grade 1 ischaemia in 2 patients. Fifteen patients with grade 3 ischaemia underwent colectomy (Hartmann's procedure) with a mortality rate of 66%. All non operated grade 3 patients died. Overall, 16 of the 28 patients died at hospital (57% mortality rate). None of the patients with mild (grade 2 or 1) colonic ischaemia died. Profound hypovolaemic shock and inflammatory AAA were the only significant predisposing factors leading to colonic ischaemia. Associated colon revascularization could not avoid the evolution to colon necrosis in four patients. Reimplantation of a patent inferior mesenteric artery or an internal iliac artery was performed in only 4.8% of all aortoiliac reconstructions, and did not influence the development of ischaemic colitis. The authors conclude that a more liberal use of postoperative sigmoidoscopy could allow detecting colonic ischaemia at an earlier stage and reduce ensuing mortality. A reinforced effort to restore or preserve colonic vascularization could lower the incidence of colonic ischaemia following aortoiliac surgery. [less ▲]

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See detailBooklist 1999
Pirenne, Christophe ULg

in Popular Music (2000), 19(3), 403-434

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See detailEosinophilic Bronchopneumopathy in Dogs
Clercx, Cécile ULg; Peeters, Dominique ULg; Snaps, Frédéric ULg et al

in Journal of Veterinary Internal Medicine (2000), 14(3, May-Jun), 282-91

Eosinophilic bronchopneumopathy was diagnosed in 23 young dogs. Clinical signs included cough, gagging, and retching in all dogs, dyspnea in 21 dogs (91%), and nasal discharge in 12 dogs (52%). The most ... [more ▼]

Eosinophilic bronchopneumopathy was diagnosed in 23 young dogs. Clinical signs included cough, gagging, and retching in all dogs, dyspnea in 21 dogs (91%), and nasal discharge in 12 dogs (52%). The most common radiographic findings were a moderate to severe bronchointerstitial pattern (68%, 13 of 19 dogs). Bronchoscopic findings included the presence of abundant yellow-green mucus or mucopurulent material (70%, 16 of 23 dogs) and severe mucosal thickening with an irregular or polypoid appearance (52%, 12 of 23 dogs), with partial airway closure during expiration in 3 dogs (13%). Peripheral blood eosinophilia was noted in 14 of 23 dogs (61%). Inflammatory cells in brush or bronchoalveolar lavage fluid cytologic preparations comprised more than 50% eosinophils in 14 of 23 dogs (61%), and 20-50% eosinophils in 6 dogs (26%). Eosinophilic infiltration of the bronchial mucosa was observed in biopsies from 19 dogs and was graded as mild (37%, 7 dogs), moderate (32%, 6 dogs), or severe (32%, 6 dogs). The mean serum immunoglobulin A concentration was almost double that of a population of 20 healthy dogs of various breeds. Oral glucocorticoids were administered on alternate days with progressive tapering of the dose; the dosage at maintenance varied between 0.1 and 1.0 mg/kg every other day. No relationship was found between the duration of clinical signs and the maintenance dosage or the cytologic and histopathologic grades. [less ▲]

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See detailHigh-energy forward scattering and the pomeron: Simple pole versus unitarized models
Cudell, Jean-René ULg; Ezhela, V.; Kang, K. et al

in Physical Review. D : Particles and Fields (2000), 61

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See detailActualité choisie du TIG et de la formation
Dantinne, Michaël ULg; Van Doosselaere, Denis

in Revue de Droit Pénal et de Criminologie (2000), (11),

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See detailVery Low Surfactant Protein C Contents in Newborn Belgian White and Blue Calves with Respiratory Distress Syndrome
Danlois, F.; Zaltash, S.; Johansson, J. et al

in Biochemical Journal (2000), 351(Pt 3), 779-87

We have studied a respiratory distress syndrome (RDS) occurring in newborn calves of the Belgian White and Blue (BWB) breed that represents the large majority of beef cattle in Belgium. Pulmonary ... [more ▼]

We have studied a respiratory distress syndrome (RDS) occurring in newborn calves of the Belgian White and Blue (BWB) breed that represents the large majority of beef cattle in Belgium. Pulmonary surfactant isolated from 14 BWB newborn calves that died from RDS and from 7 healthy controls was analysed for composition and surface activity. An extremely low content or, in some instances, an absence of surfactant protein C (SP-C) was detected in the RDS samples by Western blotting and differential amino acid analysis [0.03+/-0.01% (w/w) relative to total phospholipids, compared with 0.39+/-0.06% for healthy controls (means+/-S.E.M., P < 0.001)]. The contents of surfactant protein B (SP-B) were similar in RDS and control samples. The crude surfactant samples isolated from RDS calves had higher ratios of total protein to total phospholipid, altered phospholipid profiles and lower SP-A contents. Both crude and organic extracts of RDS surfactant samples showed increased dynamic surface tension compared with healthy controls when evaluated with a pulsating-bubble surfactometer. The addition of purified SP-C to organic extracts of RDS surfactant samples lowered surface tension. Strongly decreased levels of mature SP-C associated with fatal RDS and altered surface activity in vitro have, to the best of our knowledge, not been previously reported. The mechanisms underlying RDS and the decrease in SP-C in BWB calves remain to be established. [less ▲]

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See detailWegwijs in de vele benamingen van de veelvormige pathogeen Escherichia coli
Mainil, Jacques ULg

in Vlaams Diergeneeskundig Tijdschrift (2000), 69

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See detailTherapeutic and chemical developments of cholecystokinin receptor ligands
De Tullio, Pascal ULg; Delarge, J.; Pirotte, Bernard ULg

in Exp. Opin. Invest. Drugs (2000), 9

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See detailRecension: "Vade-Mecum de l'évaluation médico-légale"
Kohl, Benoît ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2000)

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See detailCompétition et choix du partenaire sexuel chez les primates
Huynen, Marie-Claude ULg

in Cahiers d'Ethologie (2000), 20(1), 85-112

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See detailQSO mass constraints from gravitational lensing studies of quasar pairs. The cases of Q1548+114 A & B and Q1148+0055 A & B
Claeskens, Jean-François ULg; Lee, D.-W.; Remy, M. et al

in Astronomy and Astrophysics (2000), 356

New constraints on the mass of quasars are derived from gravitational lensing studies of the QSO pairs Q1548+114 A & B and Q1148+0055 A & B, for which new ground-based and Hubble Space Telescope (HST ... [more ▼]

New constraints on the mass of quasars are derived from gravitational lensing studies of the QSO pairs Q1548+114 A & B and Q1148+0055 A & B, for which new ground-based and Hubble Space Telescope (HST) direct imagery have been obtained. In the case of Q1548+114 A & B, QSO A has been resolved into its host galaxy and a close companion. The non-detection with HST of a secondary lensed image of the background QSO in the close vicinity of the foreground one and the modeling of the host of QSO A, of the companion and of field galaxies with Singular Isothermal Spheres (SIS) yield a robust upper limit on the central compact mass of 4.5 10[SUP]11[/SUP] M[SUB]sun[/SUB]. On the other hand, the combined mass of Q1148+0055 B plus host must be smaller than 6.5 10[SUP]11[/SUP] M[SUB]sun[/SUB] since no secondary lensed image has been detected with HST. Photometry and relative astrometry of all the detected objects are reported. Based on data collected with the Hubble Space Telescope and at the European Southern Observatory, La Silla, Chile [less ▲]

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See detailConstruction and characterization of an ovine BAC contig spanning the callipyge locus.
SEGERS, Karin ULg; Vaiman, D.; Berghmans, S. et al

in Animal Genetics (2000), 31(6), 352-9

We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly ... [more ▼]

We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7.5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not1 sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation. [less ▲]

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See detailA Fast Method To Predict Protein Interaction Sites From Sequences
Gallet, X.; Charloteaux, Benoît ULg; Thomas, Annick ULg et al

in Journal of Molecular Biology (2000), 302(4), 917-26

A simple method for predicting residues involved in protein interaction sites is proposed. In the absence of any structural report, the procedure identifies linear stretches of sequences as "receptor ... [more ▼]

A simple method for predicting residues involved in protein interaction sites is proposed. In the absence of any structural report, the procedure identifies linear stretches of sequences as "receptor-binding domains" (RBDs) by analysing hydrophobicity distribution. The sequences of two databases of non-homologous interaction sites eliciting various biological activities were tested; 59-80 % were detected as RBDs. A statistical analysis of amino acid frequencies was carried out in known interaction sites and in predicted RBDs. RBDs were predicted from the 80,000 sequences of the Swissprot database. In both cases, arginine is the most frequently occurring residue. The RBD procedure can also detect residues involved in specific interaction sites such as the DNA-binding (95 % detected) and Ca-binding domains (83 % detected). We report two recent analyses; from the prediction of RBDs in sequences to the experimental demonstration of the functional activities. The examples concern a retroviral Gag protein and a penicillin-binding protein. We support that this method is a quick way to predict protein interaction sites from sequences and is helpful for guiding experiments such as site-specific mutageneses, two-hybrid systems or the synthesis of inhibitors. [less ▲]

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See detailCurrent and future trends in groundwater vulnerability assessment
Gogu, Radu; Dassargues, Alain ULg

in Environmental Geology (2000), 39(6), 549-559

The concept of groundwater vulnerability is a useful tool for environmental planning and decision-making. Many different methods have been developed for assessing this vulnerability. Hydrogeologists have ... [more ▼]

The concept of groundwater vulnerability is a useful tool for environmental planning and decision-making. Many different methods have been developed for assessing this vulnerability. Hydrogeologists have failed to reach a consensus concerning the definitions of and reference terms for groundwater vulnerability assessment. Therefore, a review of vulnerability assessment and mapping methods providing a new classification system is necessary. This is focused on techniques that use the overlay and index class methods. New research challenges in vulnerability assessment are identified, especially the need for developing dynamic links between numerical models and overlay and index methods. [less ▲]

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See detailLe maître de Waha : un exemple de développement durable
Van Ruymbeke, Muriel ULg

in De la Meuse à l'Ardenne (2000), (31), 104-106

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See detail"J'ai deux ‘amours’…"
Dejasse, Erwin ULg

in Art&Fact (2000), 19

The representations of Paris into the comic "Adèle Blanc-Sec" by Jacques Tardi.

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See detailLa néosporose chez la chèvre : résultats de deux enquêtes sérologiques dans l'Ouest de la France
Chartier, C.; Baudry, C.; Losson, Bertrand ULg et al

in Point Vétérinaire (2000), 31

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See detailImmunocastration of farm animals
Portetelle, Daniel ULg; Haezebroeck, Valérie; Mortiaux, Frédéric et al

in Bases (2000), 4

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