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See detailLacunarity, fractal, and magnetic transition behaviors in a generalized Eden growth-process
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1994), 50(2), 635-638

The effect of an internal extra degree of freedom introduced into the simplest growth model (the Eden model) leads to a joint physical and geometrical transition. Lacunarity, fractal, and magnetic ... [more ▼]

The effect of an internal extra degree of freedom introduced into the simplest growth model (the Eden model) leads to a joint physical and geometrical transition. Lacunarity, fractal, and magnetic transitions are indeed reported to be at the same critical values of the growth parameters. A logarithmic behavior of the cluster mass on this critical value was found. [less ▲]

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See detailLa déficience en hormone de croissance chez l'adulte et son traitement
Moreau, L.; Beckers, Albert ULg

in Médecine et Hygiène (1994), 52(2036), 1660-4

Les adultes déficients en hormone de croissance (GH) décèdent prématurément, principalement à cause de la fréquence élevée des affections cardiovasculaires. Depuis que la GH est produite par les ... [more ▼]

Les adultes déficients en hormone de croissance (GH) décèdent prématurément, principalement à cause de la fréquence élevée des affections cardiovasculaires. Depuis que la GH est produite par les techniques de biologie moléculaire, il est possible de proposer un traitement substitutif. Ce traitement améliore le bilan lipidique, entraîne une redistribution de la graisse d'androïde en gynoïde, une réduction de la masse graisseuse, une augmentation de la masse des tissus maigres et du volume de l'eau extracellulaire. Le poids des patients est légèrement réduit par le traitement substitutif. La masse de muscle strié squelettique est augmentée, ainsi que le contenu osseux (BMC) et la densité osseuse (BMD). La fonction cardiaque et la qualité de vie sont améliorées. [less ▲]

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See detailAcute Polymyopathy after Carbamate Poisoning in a Dog
Mc Entee, Kathleen ULg; Poncelet, Luc; Clercx, Cécile ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1994), 135(4), 88-90

The acute polymyopathy in a seven-year-old German shepherd dog was attributed to the muscular hypertonia, tremors and seizures which developed during the acute phase of carbamate poisoning. After two days ... [more ▼]

The acute polymyopathy in a seven-year-old German shepherd dog was attributed to the muscular hypertonia, tremors and seizures which developed during the acute phase of carbamate poisoning. After two days of generalised muscular rigidity, the dog adopted a characteristic fetal position which could be explained by the imbalance between the injuries to the extensor and flexor muscles. The polymyopathy resolved gradually over the course of a week. [less ▲]

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See detailInvestigation of the surface activity of a poly(styrene-b-dimethylsiloxane) copolymer blended within a polystyrene matrix
Petitjean, Serge; Ghitti, G.; Jérôme, Robert ULg et al

in Macromolecules (1994), 27

The surface properties of thin polystyrene films are deeply modified by the addition of various amounts of a poly(styrene-b-dimethylsiloxane) copolymer-or poly(S-b-DMS)-as confirmed by a series of ... [more ▼]

The surface properties of thin polystyrene films are deeply modified by the addition of various amounts of a poly(styrene-b-dimethylsiloxane) copolymer-or poly(S-b-DMS)-as confirmed by a series of analytical methods. In addition to the surface tension which has been estimated from wettability and contact angle measurements, surface composition has been analyzed by X-ray photoelectron spectroscopy (XPS) and secondary ion mass spectrometry (SIMS). Surface studies have been completed by the analysis of the depth distribution of siloxane from the surface by XPS and SIMS. Finally, the surface activity of poly(S-b-DMS) has been investigated in solution. Indeed, changes in the surface tension of polystyrene solutions in THF have been measured in relation to the addition of the diblock copolymer. All the experimental data agree with the complete coverage of the polystyrene films with the poly(S-b-DMS) copolymer. This situation already prevails in the solutions prepared as precursors to the thin polystyrene films. Accordingly, surface characteristics of film freshly prepared by solvent-casting are unaffected by subsequent annealing. [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 18. Synthesis of star-branched aliphatic polyesters bearing various functinal end groups
Tian, Dong; Dubois, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1994), 27(15), 4134-4144

Three-arm star-branched aliphatic polyesters have been synthesized by an appropriate termination reaction of the living chains prepared by ring-opening polymerization with aluminum alkoxides. The use of ... [more ▼]

Three-arm star-branched aliphatic polyesters have been synthesized by an appropriate termination reaction of the living chains prepared by ring-opening polymerization with aluminum alkoxides. The use of functional aluminum alkoxides, Et2AlOCH2X with X = CH2CH2CH=CH2 and (CH2)10Br, has led to X functional end-capped star-branched polyesters. Trimesic acid trichloride has proved to be an efficient termination agent for the living poly(epsilon-caprolactone) [PCL] chains ended with a reactive aluminum alkoxide group. A quantitative end functionalization has been reported for bromide and 1-pentene end-capped star-branched PCL. The bromide end groups have been converted into azido end groups, which have been subsequently reduced to the expected primary amine end-capped star-branched PCL by catalytic transfer hydrogenation in DMF. In contrast to living PCL, reaction of trimesic acid chloride with the aluminum alkoxide end group of living poly(L,L)lactide [P(L,L)LA] and living poly(epsilon-caprolactone-b-(L,L)lactide) [P(CL-b-(L,L)LA] chains has produced star-branched polyesters within a very poor yield. This result is more likely due to a steric effect of the methyl group in a-position of the terminal aluminum alkoxide. This drawback has been overcome to some extent by initiating the living polymerization of (L,L)LA with primary amine end-capped star-branched PCL in the presence of triethylaluminum. Formation of a hydroxyl end-capped star-branched poly(epsilon-caprolactone-b-(L,L)lactide) diblock copolymer has accordingly been reported. [less ▲]

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See detailCold Adaptation of Proteins. Purification, Characterization, and Sequence of the Heat-Labile Subtilisin from the Antarctic Psychrophile Bacillus Ta41
Davail, S.; Feller, Georges ULg; Narinx, E. et al

in Journal of Biological Chemistry (1994), 269(26), 17448-53

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal ... [more ▼]

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal amino acid sequencing of the purified enzyme indicate that the mature subtilisin S41 is composed of 309 residues with a predicted M(r) = 31,224. Subtilisin S41 shares most of its properties with mesophilic subtilisins (structure of the precursor, 52% amino acid sequence identity, alkaline pH optimum, broad specificity, Ca2+ binding) but is characterized by a higher specific activity on macromolecular substrate, by a shift of the optimum of activity toward low temperatures, and by a low thermal stability. The enzyme also differs by an acidic pI (5.3) and the presence of one disulfide bond. It is proposed that the psychrophilic enzyme possesses a more flexible molecular structure when compared to mesophilic and thermophilic subtilases in order to compensate for the reduction of reaction rates at low temperatures. The model of subtilisin S41 indeed reveals several features able to induce a more flexible, heat-labile conformation: the occurrence of four extended surface loops, a very hydrophilic surface through 11 extra Asp residues, and the lack of several salt bridges and aromatic-aromatic interactions. The low affinity of the Ca1 calcium binding site (Kd(app) = 10(-6) M), resulting possibly from one chelating side chain substitution and the stacking of Gly residues, also reflect a less compact conformation. The difference of free energy of stabilization between subtilisin S41 and a mesophilic subtilisin suggests that the balance of exo- and endothermically formed weak bonds is critical for the enzyme flexibility. [less ▲]

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See detailHubble Space Telescope Goddard high-resolution spectrograph H2 rotational spectra of Jupiter's aurora
Clarke, John T; Ben Jaffel, Lotfi; Vidal-Madjar, Alfred et al

in Astrophysical Journal (1994), 430

We have observed the emission spectrum from Jupiter's north auroral atmosphere with 0.57 A spectral resolution over 1204-1241 A. Bright emissions have been detected from 50 deg to 60 deg latitude at ... [more ▼]

We have observed the emission spectrum from Jupiter's north auroral atmosphere with 0.57 A spectral resolution over 1204-1241 A. Bright emissions have been detected from 50 deg to 60 deg latitude at locations consistent with 6 to 30 R [SUB]J[/SUB] auroral ovals, with much fainter emissions away form the auroral ovals. The emission spectrum is well fitted by both laboratory spectra and theoretical models of optically thin electron excited H2, with added Doppler-broadened Lyman Alpha emission. The observed Lyman Alpha emission wings extend more than 1 A from line center and appear correlated in strength with the H2 brightness. Individual rotational lines in the H2 Werner band system are resolved, allowing a determination of the H2 rotational temperature at the altitude of the emission. We derive best-fit temperatures from 400-450 to 700-750 K, with the auroral emission layer temperature changing either across the auroral oval or over several days' time. These observations demonstrate for the first time the ability to measure the observed rapid H2 temperature variations across Jupiter's auroral atmosphere. [less ▲]

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See detailComment je traite un hypertendu avec insuffisance rénale non diabétique
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(7), 373-374

1. L'hypertension artérielle chez l'insuffisant rénal chronique est fréquente. 2. L'hypertension artérielle accélère, dans bien des situations, la vitesse de dégradation fonctionnelle rénale. 3. Corriger ... [more ▼]

1. L'hypertension artérielle chez l'insuffisant rénal chronique est fréquente. 2. L'hypertension artérielle accélère, dans bien des situations, la vitesse de dégradation fonctionnelle rénale. 3. Corriger l'élévation de pression artérielle ralentit cette progression, en association avec les règles diététiques de régime hyposodé et hypoprotéiné. 4. Les meilleurs bénéfices semblent être obtenus pour des pressions artérielles inférieures à 130/85 mmHg. 5. Les inhibiteurs de l'enzyme de conversion apparaissent plus bénéfiques lorsqu'ils sont prescrits en présence d'une atteinte rénale légère associée à une protéinurie. Leur utilisation nécessite cependant quelques précautions (surveillance des ions et de la fonction rénale). 6. Les antagonistes calciques offrent une grande sécurité d'emploi quel que soit le degré d'atteinte rénale. Cette classe thérapeutique possède des propriétés propices au ralentissement de la dégradation fonctionnelle rénale pour toute néphropathie sans protéinurie. 7. L'association à des diurétiques (surtout de l'anse) est souvent nécessaire, le but étant de normaliser si possible la pression artérielle. [less ▲]

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See detailVariations of the Ap Stars in NGC2169
Manfroid, Jean ULg; Renson, Pierre ULg

in Information Bulletin on Variable Stars (1994), 4044

Not Available

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See detailLa chirurgie du carrefour aortique de 1975 à 1991 à Liège
Kolh, Philippe ULg; Dermiens, D.; Marnette, J. M. et al

in Revue Médicale de Liège (1994), 49(7), 397-411

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See detailHow to Measure Insulin Action in Vivo
Scheen, A. J.; Paquot, Nicolas ULg; Castillo, M. J. et al

in Diabetes/Metabolism Reviews (1994), 10(2), 151-88

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See detailRadiation-Induced Myelopathy and Vertebral Necrosis
Martin, Didier ULg; Delacollette, Mireille ULg; Collignon, Jacques ULg et al

in Neuroradiology (1994), 36(5), 405-7

Radiation-induced myelopathy is often a diagnosis of exclusion. In addition to the classic criteria needed to support the diagnosis, the presence of another radiation-induced lesion, such as aseptic ... [more ▼]

Radiation-induced myelopathy is often a diagnosis of exclusion. In addition to the classic criteria needed to support the diagnosis, the presence of another radiation-induced lesion, such as aseptic vertebral necrosis, is useful to confirm the cause of the spinal cord lesion. [less ▲]

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See detailLa Formation de Philippeville à Merlemont: contexte structural, comparaison avec le stratotype et paléoenvironnements
Boulvain, Frédéric ULg; Coen-Aubert, Marie; Dumoulin, Virginie et al

in Professional Paper (1994), 1994/2(269), 1-29921

After considering the dolomite quarry of Merlemont North in its structural and stratigraphic contexts, palaeontological (rugose corals) and sedimentological observations are presented. A ... [more ▼]

After considering the dolomite quarry of Merlemont North in its structural and stratigraphic contexts, palaeontological (rugose corals) and sedimentological observations are presented. A paleoenvironmental reconstruction is proposed and the section is compared to the stratotype of the Philippeville Formation (Belgium, Wallonia, Dinant Synclinorium, Philipeville secondary anticlinorium) [less ▲]

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See detailDepressed Immune Surveillance against Cancer: Role of Deficient T Cell: Extracellular Matrix Interactions
Gorski, A.; Castronovo, Vincenzo ULg; Stepien-Sopniewska, B. et al

in Cell Adhesion and Communication (1994), 2(3), 225-33

Although T cells infiltrate malignant tumors, the local immune response is usually inefficient and tumors escape destruction. While extracellular matrix proteins strongly costimulate T cell responses in ... [more ▼]

Although T cells infiltrate malignant tumors, the local immune response is usually inefficient and tumors escape destruction. While extracellular matrix proteins strongly costimulate T cell responses in normal individuals, our studies indicate that peripheral blood T cells from cancer patients and tumor infiltrating cells respond poorly or are resistant to stimulative signals mediated by collagen I and IV and fibronectin. Moreover, the adhesive properties of cancer T cells are markedly depressed. Those functional deficiencies are paralleled by variable deficits in integrin and non-integrin T cell receptors for extracellular matrix. Immunotherapy with BCG causes a dramatic but transient increase in T cell: ECM interactions. [less ▲]

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See detailComplement, leukocytes, and leukocyte elastase in full-term neonates undergoing cardiac operation.
SEGHAYE, Marie-Christine ULg; Duchateau, J.; Grabitz, R. G. et al

in Journal of Thoracic and Cardiovascular Surgery (The) (1994), 108(1), 29-36

In 13 neonates undergoing cardiac operations for congenital cardiac defects, complement, leukocytes, and leukocyte elastase were studied during and after cardiopulmonary bypass. All but two neonates ... [more ▼]

In 13 neonates undergoing cardiac operations for congenital cardiac defects, complement, leukocytes, and leukocyte elastase were studied during and after cardiopulmonary bypass. All but two neonates received prostaglandin E1 before the operation. The C3d/C3 ratio rose significantly during cardiopulmonary bypass from 0.86 +/- 0.55 to 1.40 +/- 0.56 (mean +/- standard deviation; p < 0.0001). Abnormally elevated C5a levels (18.6 +/- 7.3 micrograms/L) were measured at the end of cardiopulmonary bypass. C4 was not overtly consumed during the procedure. Leukocytes fell from a preoperative value of 10.06 +/- 3.15 x 10(9)/L to 3.21 +/- 0.64 x 10(9)/L after beginning of cardiopulmonary bypass (p < 0.0001) and rose at the end of the procedure from 2.33 +/- 0.67 x 10(9)/L to 7.19 +/- 1.84 x 10(9)/L, after protamine administration (p < 0.0001). Neutrophils fell from a preoperative value of 5.14 +/- 1.18 x 10(9)/L to 1.46 +/- 0.35 x 10(9)/L after beginning of cardiopulmonary bypass and rose at the end of extracorporeal circulation from 1.00 +/- 0.31 x 10(9)/L to 4.10 +/- 1.18 x 10(9)/L, after protamine administration (p < 0.005). Elastase release occurred in all neonates during cardiopulmonary bypass and averaged 331.5 +/- 175.7 micrograms/L. Complement activation and leukocyte stimulation did not correlate with postoperative complications or outcome. This study demonstrates complement activation and leukocyte stimulation in neonates undergoing cardiac operation. [less ▲]

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See detailIsolation and characterization of preantral follicles from fetal bovine ovaries
Hulshof, S. C. J.; Figueiredo, José Ricardo; Beckers, Jean-François ULg et al

in Veterinary Quarterly (The) (1994), 16(2), 78-80

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The ... [more ▼]

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter. [less ▲]

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See detailImpossible reconversion de l’industrie militaire russe
Bachkatov, Nina ULg

in Monde Diplomatique (Le) : Journal des Cercles Diplomatiques et des Grandes Organisations Internationales (1994)

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See detailHigh-field magnetization of U2T2X compounds (T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn)
Nakotte, Heinz; Prokes, Karel; Brück, E. et al

in Physica B: Condensed Matter (1994), 201

Structure parameters and magnetization in quasistatic fields up to 38 T at 4.2 K are reported for U2T2X compounds with T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn, which crystalline in the tetragonal U3Si2 ... [more ▼]

Structure parameters and magnetization in quasistatic fields up to 38 T at 4.2 K are reported for U2T2X compounds with T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn, which crystalline in the tetragonal U3Si2 structure. Even the highest applied fields are not sufficient to achieve saturation of the magnetization in any of these compounds of which the majority is antiferromagnetic and some are Pauli paramagnets. The importance of 5f-ligand hybridization for the magnetism in these compounds is discussed. [less ▲]

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See detailHygiène cutanée
Kort, R.; Nikkels, Arjen ULg; Pierard, Gérald ULg

in Journal d'Actualités Dermatologiques Belges (1994), 12

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See detailAnionic polymerization of acrylic monomers. 14. Carbon-13 and lithium-7 NMR characterization of the complexation of methyl α-lithioisobutyrate with various lithium cation-binding ligands in tetrahydrofuran
Wang, Jin-Shan; Jérôme, Robert ULg; Warin, R. et al

in Macromolecules (1994), 27(12), 3376-3382

The aggregation equilibrium and electronic structure of methyl alpha-lithioisobutyrate (MIBLi), a monomeric model structurally corresponding to a living poly(methyl methacrylate) chain end associated with ... [more ▼]

The aggregation equilibrium and electronic structure of methyl alpha-lithioisobutyrate (MIBLi), a monomeric model structurally corresponding to a living poly(methyl methacrylate) chain end associated with a lithium countercation, has been examined by Li-7 and/or C-13 NMR spectroscopy in tetrahydrofuran (THF) in the presence of various lithium cation-binding ligands. The addition of dimethoxyethane (DME), triglyme (glyme-3),12-crown-4 (12-CE-4), and hexamethylphosphoric triamide (HMPA) to coexisting tetrameric and dimeric MIBLi in THF induces an increase in dimeric population in the order DME < glyme-3 < 12-CE-4 <HMPA. That order corresponds to an increasing strength of complexation between MIBLi (lithium cation) and the (solvating) ligands For either tetrameric or dimeric species, moreover, the negative charge distribution around MIBLi does not seem to be significantly modified due to the presence of these complexing agents. In striking contrast, complexation of MIBLi by cryptand 211 (K211) does shift the equilibrium between tetramer and dimer toward the formation of a single monomeric K211-complexed species, which gives rise to ca. 13 and 5 ppm upfield shifts at C(alpha) in the C-13 NMR spectra, compared to tetrameric and dimeric species, respectively. The effect of 12-CE-4/K211 on the original aggretation equilbrium for MIBLi in THF nicely explains the significantly different MWD of PMMA anionically prepared in the presence of each of these ligands. [less ▲]

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See detailLe diabète de type 2 insulinorequérant: caractéristiques des patients et effets de l'insulinothérapie
Duysinx, Bernard ULg; Scheen, A. J.; Paquot, Nicolas ULg et al

in Revue Médicale de Liège (1994), 49(6), 305-23

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See detailComment je traite un patient hypertendu âgé
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(6), 303-304

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées ... [more ▼]

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées à plusieurs reprises et trouvées dépassant les valeurs de 160et/ou 95 mmHg, en raison de la démonstration de l'efficacité certaine de ce traitement dans la diminution de l'incidence des complications cardiovasculaires du patient hypertendu âgé. 2. Le régime sans sel strict doit être évité. Une correction de l'excès de poids doit bien sûr être associée au traitement médicamenteux. 3. Pour le traitement médicamenteux, il est nécessaire de choisir des agents antihypertenseurs dont l'utilisation est bien connue du prescripteur. 4. Ne pas viser d'emblée l'efficacité maximale. Il est conseillé d'augmenter la posologie par palliers successifs. 5. Une surveillance attentive du patient est nécessaire avec recherche des effets secondaires médicamenteux. 6. Si le traitement est inefficace, il est nécessaire de remettre en question ce premier choix. Celui-ci est classiquement un diurétique ou, en cas de contre-indication, un antagoniste calcique. 7. Le schéma thérapeutique doit être facile à comprendre du patient et consister en une prise unique. 8. Rappelons que le but du traitement est de prolonger l'existence du patient tout en assurant une qualité de vie correcte. [less ▲]

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See detailStability and Structural Analysis of Alpha-Amylase from the Antarctic Psychrophile Alteromonas Haloplanctis A23
Feller, Georges ULg; Payan, F.; Theys, F. et al

in European Journal of Biochemistry (1994), 222(2), 441-7

The alpha-amylase secreted by the antarctic bacterium Alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. The psychrophilic alpha-amylase is however ... [more ▼]

The alpha-amylase secreted by the antarctic bacterium Alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. The psychrophilic alpha-amylase is however characterized by a sevenfold higher kcat and kcat/Km values at 4 degrees C and a lower conformational stability estimated as 10 kJ.mol-1 with respect to the porcine enzyme. It is proposed that both properties arise from an increase in molecular flexibility required to compensate for the reduction of reaction rates at low temperatures. This is supported by the fast denaturation rates induced by temperature, urea or guanidinium chloride and by the shift towards low temperatures of the apparent optimal temperature of activity. When compared with the known three-dimensional structure of porcine pancreatic alpha-amylase, homology modelling of the psychrophilic alpha-amylase reveals several features which may be assumed to be responsible for a more flexible, heat-labile conformation: the lack of several surface salt bridges in the (beta/alpha)8 domain, the reduction of the number of weakly polar interactions involving an aromatic side chain, a lower hydrophobicity associated with the increased flexibility index of amino acids forming the hydrophobic clusters and by substitutions of proline for alanine residues in loops connecting secondary structures. The weaker affinity of the enzyme for Ca2+ (Kd = 44 nM) and for Cl- (Kd = 1.2 mM at 4 degrees C) can result from single amino acid substitutions in the Ca(2+)-binding and Cl(-)-binding sites and can also affect the compactness of alpha-amylase. [less ▲]

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See detailThe use of gravitational microlensing to scan the structure of BAL QSOs
Hutsemekers, Damien ULg; Surdej, Jean ULg; van Drom, E.

in Astrophysics & Space Science (1994), 216

Approximately 10% of the quasi-stellar objects (QSOs) show broad absorption lines (BAL) in their spectra which, if interpreted in terms of Doppler velocities, reveal the presence of high velocity gas ... [more ▼]

Approximately 10% of the quasi-stellar objects (QSOs) show broad absorption lines (BAL) in their spectra which, if interpreted in terms of Doppler velocities, reveal the presence of high velocity gas outflows. One of these BAL QSOs is known to be gravitationally lensed. It therefore constitutes a good candidate to search for microlensing effects, i.e., the selective amplification of different line forming regions. Considering current models for the BAL region, we have investigated the effects of moving microlenses on the line profiles, and we conclude that these effects strongly depend on the adopted model. A regular spectroscopic monitoring of lensed BAL QSOs would therefore be highly valuable to distinguish between the various models proposed so far to interpret the origin of broad absorption lines. [less ▲]

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See detailExpression of Bone Sialoprotein, a Bone Matrix Protein, in Human Breast Cancer
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Castronovo, Vincenzo ULg

in Cancer Research (1994), 54(11), 2823-6

Microcalcifications are often associated with human mammary lesions, particularly with breast carcinomas. To date, the molecular mechanism that leads to the deposition of hydroxyapatite in the mammary ... [more ▼]

Microcalcifications are often associated with human mammary lesions, particularly with breast carcinomas. To date, the molecular mechanism that leads to the deposition of hydroxyapatite in the mammary tissue has not been elucidated. Bone sialoprotein (BSP) is a glycoprotein the expression of which coincides with the appearance of the first hydroxyapatite crystals during bone development. In this study, we report the observation that BSP, a bone matrix protein, is expressed in human mammary cancer cells. Using an immunoperoxidase technique, we studied the expression of BSP in 79 breast lesions, including 28 benign and 51 malignant specimens. Two polyclonal antibodies, one directed against intact human BSP and the other against a synthetic peptide of BSP (residues 277-294), were used and gave identical results. Normal mammary glands expressed undetectable or barely detectable amounts of BSP, and the majority of the benign lesions examined were generally unstained (0) or weakly stained (1+). Most of the breast carcinoma specimens (around 87%) showed a significant increase (P = 0.0001) in BSP expression. Breast carcinomas with microcalcifications had the highest immunoreactivity (2+ or 3+) to BSP antibodies. This is the first demonstration that BSP expression is significantly increased in breast cancer. Expression of BSP by breast cancer cells could play a major role in the deposition of microcalcifications and in the preferred bone homing of breast cancer cells. [less ▲]

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See detailNumerical Procedure for Calculation of Temperature Evolution in Composite Elements with Internal Cavities
Luycks, Pierre; Stienon, P.; Franssen, Jean-Marc ULg et al

in Communications in Numerical Methods in Engineering (1994), 10(6), 437-451

In the paper a computation code based on the finite-element method for temperature evolutions in composite elements with internal cavities is presented. It is suitable for the analysis of various thermal ... [more ▼]

In the paper a computation code based on the finite-element method for temperature evolutions in composite elements with internal cavities is presented. It is suitable for the analysis of various thermal problems encountered in mechanical and civil engineering such as fire risk assessment. It is based on a plane isoparametric triangular element with six nodes allowing the modelling of curved boundaries. In order to analyze composite steel-concrete elements and to take into account the non-linearities coming from the temperature-dependent material properties and from the boundary conditions, an implicit integration scheme is used. Another advantage of the program is the possibility of describing internal cavities with convection and radiation exchanges. In order to show the capacity of the program two examples are presented. Comparisons have been made with experimental and other numerical results, exhibiting very good agreement. [less ▲]

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See detailChiral Separation of Basic Drugs by Capillary Zone Electrophoresis with Cyclodextrin Additives
Bechet, I.; Paques, P.; Fillet, Marianne ULg et al

in Electrophoresis (1994), 15(6), 818-23

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and ... [more ▼]

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and uncoated fused silica capillaries thermostated at 15 degrees C. The nature of the cationic component of the background electrolyte was found to have a significant influence on achiral resolution and peak symmetry. The best results were obtained with triethanolamine, which was then used to adjust the buffer pH in all further experiments. The effects on chiral resolution of the nature and concentration of cyclodextrin, of the addition of methanol, and of capillary temperature were studied. Maximum resolution was obtained at a particular cyclodextrin concentration for each analyte, depending on the affinity of the analyte for this cyclodextrin. On the basis of the results, the effects of methanol addition and temperature on enantiomeric resolution could be explained and predicted. Numerous chiral separations are presented and suggestions for the rapid optimization of CZE enantioseparations with cyclodextrin additives are given. [less ▲]

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See detailAcquisition of new concepts by two amnesic patients
Van der Linden, Martial ULg; Meulemans, Thierry ULg; Lorrain, D.

in Cortex : A Journal Devoted to the Study of the Nervous System & Behavior (1994), 30(2), 305-317

Two Korsakoff amnesics (A.G. and G.S.) and two control subjects were taught six new concepts. Each concept was composed of three parts: the name of the concept, the context in which the concept originated ... [more ▼]

Two Korsakoff amnesics (A.G. and G.S.) and two control subjects were taught six new concepts. Each concept was composed of three parts: the name of the concept, the context in which the concept originated and its definition. The learning procedure consisted of two phases: (1) learning the concept names and definitions by means of the vanishing-cues method; (2) practice on examples of the concepts through a classification task: examples were either set in the same context as that given in the original definition or in mixed contexts (same and new contexts). Subjects were then tested after 24 hours, a week and a month on their ability to identify new examples as belonging to one of the conceptual rules studied (transfer tests). Both patients showed substantial learning. Patient A.G. was slow and dependent of the first letter cues in the vanishing-cues learning phase but nevertheless, she acquired a large and flexible conceptual knowledge and this was especially true for concepts that were practised by means of mixed-context examples. Patient G.S. easily learned to associate the definitions with the concept names but her conceptual knowledge remained more limited. These results confirm the existence of a semantic learning ability in amnesic patients. They also suggest that under appropriate learning conditions, amnesics may eventually acquire a new flexible conceptual knowledge. [less ▲]

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See detailEnseigner en 1994, est-ce une mission impossible ?
Beckers, Jacqueline ULg

in Education-Formation (1994)

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See detailAfferent and Efferent Connections of the Sexually Dimorphic Medial Preoptic Nucleus of the Male Quail Revealed by in Vitro Transport of Dii
Balthazart, Jacques ULg; Dupiereux, V.; Aste, N. et al

in Cell & Tissue Research (1994), 276(3), 455-75

The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this ... [more ▼]

The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this nucleus in the control of reproduction requires the identification of its afferent and efferent connections. In order to identify neural circuits involved in the control of the medial preoptic nucleus, we used the lipophilic fluorescent tracer DiI implanted in aldheyde-fixed tissue. Different strategies of brain dissection and different implantation sites were used to establish and confirm afferent and efferent connections of the nucleus. Anterograde projections reached the tuberal hypothalamus, the area ventralis of Tsai, and the substantia grisea centralis. Dense networks of fluorescent fibers were also seen in several hypothalamic nuclei, such as the anterior medialis hypothalami, the paraventricularis magnocellularis, and the ventromedialis hypothalami. A major projection in the dorsal direction was also observed from the medial preoptic nucleus toward the nucleus septalis lateralis and medialis. Afferents to the nucleus were seen from all these regions. Implantation of DiI into the substantia grisea centralis also revealed massive bidirectional connections with a large number of more caudal mesencephalic and pontine structures. The substantia grisea centralis therefore appears to be an important center connecting anterior levels of the brain to brain-stem nuclei that may be involved in the control of male copulatory behavior. [less ▲]

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See detailSchimmeldiagnostiek: Nieuwe technieken
Pierard, Gérald ULg; Nikkels, Arjen ULg

in Janssen Medisch-Wetenschappelijk Nieuws (1994), 9

De diagnose schimmelinfectie van de huid of de nagel kan bevestigd worden met een KOH-preparaat (microscopie) of met een kweek. Beide methoden kunnen een vals-negatieve of vals-positieve iutslag hebben ... [more ▼]

De diagnose schimmelinfectie van de huid of de nagel kan bevestigd worden met een KOH-preparaat (microscopie) of met een kweek. Beide methoden kunnen een vals-negatieve of vals-positieve iutslag hebben. Nieuwe techniken - immunohistochemie en flow-cytometrue - kunnen een aanvulling vormen op de bestaande methoden. Vooral voor de identificatie van schimmels kunnen deze methoden waardevol zijn. Voorlopig zijn deze technieken alleen in gebruik voor wetenschappelijk onderzoek. Ze leveren een belangrijke bijdrage aan de kennis over de etiologie van schimmelinfecties. Zo komt er steeds meer bewijs dat nagelinfecties lang niet altijd worden veroorzaakt door dermatofyten, maar dat ook gisten en overige schimmels pathogeen kunnen zijn voor de nagel. [less ▲]

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See detailLa aricultura en el espacio rural europeo
Mormont, Marc ULg

in Agricultura y Sociedad (1994), (No. 71), 17-49

The current crisis in EU agriculture, which is being forced to develop new structures to contend with international competition, could be seen as an entirely economic sectoral crisis. However, the ... [more ▼]

The current crisis in EU agriculture, which is being forced to develop new structures to contend with international competition, could be seen as an entirely economic sectoral crisis. However, the repercussions are in fact widespread, resulting in social change and a profound identity crisis. This article examines the relationship between agriculture and the rural sector, and shows that many traditional agricultural/rural links have been broken due to the economic and commercial development of agriculture, leading to increasing marginalization of many rural areas and extensive rural depopulation. The effects on the environment are also considered. It is concluded that rural areas must become areas to live in, and rather than being dependent on agriculture they must develop in harmony with it. [less ▲]

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See detailInfective Endocarditis: Prevention, Diagnosis and Management
Pierard, Luc ULg; LANCELLOTTI, Patrizio ULg; Galiuto, L.

in European Journal of Emergency Medicine (1994), 1(2), 104-9

Infective endocarditis remains an important problem and the means of prevention are still insufficient. The causal bacteria have changed very little, but the incidence of nosocomial infections and ... [more ▼]

Infective endocarditis remains an important problem and the means of prevention are still insufficient. The causal bacteria have changed very little, but the incidence of nosocomial infections and endocarditis complicating intravenous drug abuse are increasing. The distinction between subacute and acute clinical presentations remains appropriate. Cardiac and neurological complications are frequent and carry a high risk of mortality. The diagnosis is obtained by the integration of clinical data and the results of blood cultures. Echocardiography is extremely useful for detecting vegetations, and for assessing the haemodynamic consequences and specific cardiac complications. Risk stratification can be obtained by correct integration of multiple parameters. The causal agent should be identified before the initiation of antimicrobial therapy. Surgery is frequently required, and should be performed rapidly when indicated. [less ▲]

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See detailThe Er-Tr4 Monoclonal Antibody Recognizes Murine Thymic Epithelial Cells (Type 1) and Inhibits Their Capacity to Interact with Immature Thymocytes: Immuno-Electron Microscopic and Functional Studies
Defresne, Marie-Paule ULg; Nabarra, B.; van Vliet, E. et al

in Histochemistry (1994), 101(5), 355-63

The thymic stroma is heterogeneous with regard to cellular morphology and cellular function. In this study, we employed the monoclonal antibody ER-TR4 to characterize stromal cells at the ultrastructural ... [more ▼]

The thymic stroma is heterogeneous with regard to cellular morphology and cellular function. In this study, we employed the monoclonal antibody ER-TR4 to characterize stromal cells at the ultrastructural level. To identify the labelled cell type, we used two techniques: immunogold labelling on ultrathin frozen sections and immunoperoxidase staining on thick "vibratome" sections. ER-TR4 reacted with thymic Type 1 epithelial cells (according to our classification). A dense labelling appears in the cytoplasm of cortical cells using the two techniques. Immunogold labelling identified small cytoplasmic vesicles whereas the cytoplasm and the cell membrane seem to be labelled with the immunoperoxidase technique. ER-TR4 also identified isolated thymic nurse cells (TNC), and was observed in vitro to inhibit the capacity of some type 1 epithelial cells to establish interactions with immature thymocytes. This finding supports the hypothesis that the factor is involved in the formation of lymphoepithelial interactions within thymic nurse cells, and thus in the relations that immature thymocytes establish with the thymic microenvironment. [less ▲]

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See detailInverse Expression of Two Laminin Binding Proteins, 67lr and Galectin-3, Correlates with the Invasive Phenotype of Trophoblastic Tissue
van den Brule, F. A.; Price, J.; Sobel, M. E. et al

in Biochemical and Biophysical Research Communications (1994), 201(1), 388-93

Tumor invasion of host tissues and trophoblastic penetration of the endometrium share common biological features. Both processes involve the invasion of basement membranes, an event that is initiated by ... [more ▼]

Tumor invasion of host tissues and trophoblastic penetration of the endometrium share common biological features. Both processes involve the invasion of basement membranes, an event that is initiated by adhesion of cancer or trophoblast cells to basement membrane components and particularly to laminin. Adhesion to this latter glycoprotein is mediated through a variety of cell surface receptors. We have previously shown that the 67 kD Laminin Receptor (67LR) and a 31 kD Human Laminin Binding Protein, recently renamed galectin-3, are inversely modulated as the invasive phenotype of cancer cells progresses, with up regulation of the former, and down regulation of the latter, respectively. In this study, we examined the expression of these two proteins in 27 human trophoblastic specimens at different gestational ages using Northern and Western blot techniques. Expression of the 67LR increased from 7 weeks to a maximum at 12 weeks, when invasion is maximal, and then decreased. Expression of galectin-3 was inversely modulated by the gestational age, with a minimum expression at 12 weeks. Our data demonstrate that invasive trophoblast displays the same pattern of laminin binding proteins expression than invasive cancer cells, and further demonstrates that invasion of the extracellular matrix by trophoblast and cancer cells share common molecular mechanisms. [less ▲]

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See detailTestosterone Effects on Neurotensin-Immunoreactive Cells in the Quail Preoptic Area
Absil, Philippe ULg; Balthazart, Jacques ULg

in Neuroreport (1994), 5(9), 1129-32

In the quail preoptic area (POA), neurotensin-immunoreactive (NT-ir) cells are mostly located in the sexually dimorphic medial preoptic nucleus (POM). They are more numerous in females than in males and ... [more ▼]

In the quail preoptic area (POA), neurotensin-immunoreactive (NT-ir) cells are mostly located in the sexually dimorphic medial preoptic nucleus (POM). They are more numerous in females than in males and, in females, their number varies during the ovulatory cycle. This suggests a control by steroids of NT expression in the POA. This idea was tested by comparing the NT-ir populations in gonadectomized males and females treated or not with testosterone. No sex differences in the NT-ir cell number was present in gonadectomized birds. Testosterone treatment increased the number of immunoreactive perikarya. This effect was anatomically specific and the increase was observed in the area caudal to the anterior commissure but not in POM itself. [less ▲]

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See detailPrimary cerebral lymphoma. A retrospective study of 27 cases
Grangier, C; COUCKE, Philippe ULg; Baur, A et al

in Strahlentherapie und Onkologie (1994), 170(4), 206-212

Primary cerebral lymphoma is a rare disease. The aim of this study is to compare the survival of the patients treated with radiotherapy alone vs. patients treated with a combined schedule of radio ... [more ▼]

Primary cerebral lymphoma is a rare disease. The aim of this study is to compare the survival of the patients treated with radiotherapy alone vs. patients treated with a combined schedule of radio-chemotherapy. Our results will be compared with currently published data and main prognostic factors will be briefly discussed. PATIENTS AND METHODS: Between 1974 and 1990, 27 cases of primary cerebral lymphoma were diagnosed at our institution. All patients had biopsy-proven disease, the pathology of which was reviewed for this study. RESULTS: The overall median survival time was 24 months and one-, two- and three-year overall survival was 59, 46 and 29% respectively. The median radiation dose was 46 Gy, ranging from 19.5 to 60 Gy. The median dose per fraction was 2 Gy (ranging from 1.61 to 3 Gy). The median elapsed treatment time was 32 days (ranging from three to 45 days). We were not able to demonstrate any statistically significant difference between patients who received radiotherapy alone (n = 14, median survival time = 24 months) and those who received a combination of chemotherapy and radiotherapy (n = 11, median survival time = 30 months), (p = 0.4). Prognostic factors of survival were tested using a univariate analysis (Wilcoxon test). Parameters such as mass appearance (unilobular, p = 0.048), performance status at the time of the diagnosis (0 to 1, p = 0.014), and CT imaging (hypodense, p = 0.043) influenced positively survival. Centroblastic histology (Kiel) was found associated with a negative prognosis (p = 0.043). CONCLUSION: In our experience, there is no statistically significant difference of survival between patients treated with radiotherapy alone or with a combined treatment of radio-chemotherapy. Other prognostic factors of survival were discovered, although the analysis was univariate, due to the limited number of patients. Multicentric prospective studies should be elaborated in order to optimize the treatment of this disease. [less ▲]

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See detailCalcitonine en prévention et traitement de l'ostéoporose
Reginster, Jean-Yves ULg

in Revue Médicale de Liège (1994), 49(5), 253-9

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See detailPeriod of the Si-star in NGC 2169
Renson, Pierre ULg

in Astronomy and Astrophysics (1994), 285

For the Si Ap-star known in NGC 2169 (Gr.9 in the cluster), a period between 0.60 and 0.62 d, most likely about 0.606 d, agrees with all known observations, and is much more probable than the period 1.56 ... [more ▼]

For the Si Ap-star known in NGC 2169 (Gr.9 in the cluster), a period between 0.60 and 0.62 d, most likely about 0.606 d, agrees with all known observations, and is much more probable than the period 1.56 d proposed by Maitzen & Lebzelter (1993). The obtained period is among the shortest ones known for Ap stars. [less ▲]

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See detailLa protéine P53, protectrice de l'intégrité du génome: rôle dans la genèse des cancers
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Gielen Jacques et al

in Revue Médicale de Liège (1994), 49(5), 274-84

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et ... [more ▼]

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et déterminent leur appartenance tissulaire. Des données récentes indiquent que l'apparition des cancers résulte du déséquilibre de cet état dynamique soit part l'activation de facteurs positifs désignés sous le terme d'oncogènes, soit par l'inactivation de facteurs négatifs qui sont regroupés dans la famille des gènes suppresseurs de tumeurs. Le gène codant pour la protéine p53 est un membre particulièrement important de cette dernière famille. En effet, son inactivation, par mutation et/ou délétion, est l'une des altérations génétiques la plus fréquemment détectée dans les cancers. La fonction principale attribuée à la protéine p53 consiste à préserver le génome des altérations susceptibles d'entraîner, entre autres, la cellule dans un processus de transformation maligne. C'est en permettant la réparation des altérations du DNA survenues lors de sa réplication avant la mitose ou provoquées par des agents extérieurs, que la protéine p53 semble exercer ses fonctions. Des altérations du gène p53 entraînant des perturbations de la fonction de la protéine p53 ont été identifiées au niveau de la plupart des lésions tumorales malignes. A ce titre, cette protéine semble jouer un rôle déterminant au niveau de la genèse des cancers. Aussi le gène p53 fait-il l'objet de recherches intensives qui devraient déboucher sur la mise au point de nouveaux moyens de détection et d'évaluation pronostique des cancers. D'autre part, des expériences visant à restaurer la fonction de la protéine p53 au niveau des cellules cancéreuses ouvrent de nouvelles et séduisantes perspectives thérapeutiques. [less ▲]

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See detailEfficacy and Tolerability of a New Formulation of Oral Tiludronate (Tablet) in the Treatment of Paget's Disease of Bone
Reginster, Jean-Yves ULg; Treves, R.; Renier, J. C. et al

in Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research (1994), 9(5), 615-9

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial ... [more ▼]

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial. Patients received a daily dose of 400 mg oral tiludronate (two tablets). Treatment was for 6 months. Serum alkaline phosphatase activity (SAP) and fasting urinary excretion of hydroxyproline/creatine (OH/Cr) were measured every 3 months, as were biochemical parameters reflecting renal, hepatic, and hematologic functions. Analgesic efficacy was self-evaluated from a visual analog scale (VAS). Statistical analysis revealed a significant reduction from baseline in SAP and OH/Cr levels, as well as VAS scores. In the whole population with evaluation under treatment, there was a reduction in initial SAP activity after 3 months (47.2 +/- 2.2%, mean +/- SEM) and 6 months (58.3 +/- 2.3%). In the population with SAP levels above twice the upper limit at inclusion and with evaluation at month 3 and month 6 (n = 96), the reduction in SAP levels was 49.3 +/- 2.4% after 3 months and of 59.5 +/- 2.6% after 6 months (ANOVA time effect, p = 0.0001). Aside from mild gastrointestinal disturbances, as experienced with other oral bisphosphonates, clinical tolerance was good. Exhaustive biochemical investigation failed to reveal significant toxicity of tiludronate tablets at the dose of 400 mg/day. The dose of 400 mg daily of this new formulation appears to be a satisfactory tiludronate regimen for the treatment of Paget's disease of bone. [less ▲]

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See detailMacrophages and Tumor Necrosis Factor Alpha in Toxic Epidermal Necrolysis
Paquet, Philippe ULg; Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg et al

in Archives of Dermatology (1994), 130(5), 605-608

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell ... [more ▼]

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell infiltrate varied according to the stage of the disease. The main constant feature was the prominent involvement of the monocyte-macrophage lineage, including factor XIIIa+HLA-DR+ dendrocytes and CD68+ Mac 387+ macrophages, before and during the epidermal necrosis. The number of CD4+ and CD8+ lymphocytes was comparatively small. This was associated with a dense labeling of the epidermis for tumor necrosis factor alpha. CONCLUSIONS: Cells of the monocyte-macrophage lineage largely outnumber lymphocytes in the lesions of toxic epidermal necrolysis. Tumor necrosis factor alpha is likely a major cytokine that is responsible for necrosis. [less ▲]

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See detailDirect NMR evidence for a local resriction in the segmental chain mobility of a model ionomer
Vanhoorne, Pierre; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(9), 2548-2552

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which ... [more ▼]

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which the chain segments bearing the sodium carboxylate end groups have been selectively protonated; the second one has a protonated polystyrene backbone terminated at both ends with a deuterated polystyrene segment bearing the sodium carboxylate groups. Both polymers have been studied by high-resolution solid-state C-13 NMR. Line-width measurements of the protonated units have clearly shown that the dipolar interactions of the sodium carboxylate end groups are responsible for a mobility restriction of the chain end segments. This experiment is thought to be the first direct evidence for the model recently proposed by Eisenberg et al. for ionomer morphology according to which the ionic multiplets are surrounded with a shell of constrained polymer segments. [less ▲]

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See detailAgm-1470, a Potent Angiogenesis Inhibitor, Prevents the Entry of Normal but Not Transformed Endothelial Cells into the G1 Phase of the Cell Cycle
Antoine, Nadine ULg; Greimers, Roland ULg; De Roanne, C. et al

in Cancer Research (1994), 54(8), 2073-6

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this ... [more ▼]

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this molecule appears to be a most promising anticancer drug, its mechanism of action has not yet been elucidated. In this study, we examined the effects of AGM-1470 on the cell cycle of normal and transformed endothelial cells. We showed that AGM-1470, at picomolar concentrations, specifically inhibits the proliferation of both bovine aortic endothelial cells and human umbilical vein endothelial cells. AGM-1470 was ineffective in significantly inhibiting the proliferation of Ea.hy926 cells, a hybrid cell line obtained by the fusion of human umbilical vein endothelial cells with a human carcinoma cell line, or cEnd.1 cells, a polyoma middle T oncogene-transformed endothelioma cell line derived from mouse embryo. Using a double labeling technique with anti-Ki67 antibodies and propidium iodide, we demonstrated, with flow cytometry analysis, that AGM-1470 specifically prevents the entry of endothelial cells into the G1 phase of the cell cycle. We also showed that AGM-1470 was ineffective in inhibiting endothelial cell migration toward laminin or capillary-like tube formation inside a type I collagen matrix induced by phorbol esters. Our data strongly suggest that AGM-1470 is a molecule that specifically inhibits a cell cycle control pathway active in normal cells but which could be bypassed or altered in transformed cells. [less ▲]

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See detail4 YEARS RESEARCH ON THE REPRODUCTIVE-BEHAVIOR AND HYBRIDIZATION OF BARBUS-BARBUS AND BARBUS-MERIDIONALIS
Poncin, Pascal ULg

in Bulletin Français de la Pêche et de la Pisciculture (1994), 334

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The ... [more ▼]

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The reproductive behaviour is described as well as the behavioural mechanisms of hybridization. During the spawning act - sometimes preceded by <<forehead swim>> in which two fish swim together, at high speed, head against head the female and the few accompanying males rise from the gravel and move their genital papilla in the gravel while releasing ova or sperm (fig. 1 and 2). The reproductive behaviours of Barbus barbus and Barbus meridionalis appear to be similar and hybridizations were observed, showing that no complete behavioural barrier blocks the mating of the two species (fig. 2). The daily rhythms of activity are compared within the two species. The influence of temperature and daylength on the daily rhythms of activity is discussed. in winter conditions, barbels exhibit a night activity rhythm. Spawning occurs during daytime or during the night (fig. 3). The influence of spawning stimuli is analysed using dummies of different sizes and colors (photo I). Barbels seem to prefer white, grey, yellow and brown colors to red or black colors. [less ▲]

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See detailKnowledge-Based System for the Automated Solid-Phase Extraction of Basic Drugs from Plasma Coupled with Their Liquid Chromatographic Determination. Application to the Biodetermination of Beta-Receptor Blocking Agents
Hubert, Philippe ULg; Chiap, Patrice ULg; Moors, M. et al

in Journal of Chromatography. A (1994), 665(1), 87-99

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges ... [more ▼]

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges (DECs) by means of a sample processor. A knowledge-based system was developed to facilitate the development of fully automated methods for the solid-phase extraction of relatively hydrophobic basic drugs from plasma, coupled with their determination by high-performance liquid chromatography (HPLC). The DEC filled with 50 mg of cyanopropyl-bonded silica phase is first conditioned with methanol and buffer solution (pH 7.4). After sample application, the DEC sorbent is washed with the same buffer. The analytes are then desorbed with an appropriate eluent and the eluate is finally diluted with the same buffer as used in the HPLC mobile phase before injection. Under these conditions, only three variables are still to be optimized: the composition and volume of the elution solvent and the volume of buffer to be added to the eluate. On the basis of this general strategy, a decision tree providing information about suggested starting conditions and guidelines for the optimization of the three variables was developed and implemented by use of a hypermedia software. This didactic expert system was evaluated using several beta-receptor blocking agents as model compounds and the operating conditions obtained for the automated SPE of these compounds are presented. A method for the determination of propranolol in plasma using the SPE conditions deduced from the knowledge-based system was validated. The absolute recovery of propranolol is ca. 93% and the limit of detection is 1.3 ng ml-1. The mean within-day and between-day reproducibilities are 2.3 and 3.6%, respectively. [less ▲]

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See detailIncreased Parasellar Activity on Gallium Spect Is Not Specific for Active Cluster Headache
Sianard-Gainko, J.; Milet, J.; Ghuysen, V. et al

in Cephalalgia : An International Journal of Headache (1994), 14(2), 132-3

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients ... [more ▼]

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients, 54% of episodic CH patients in an active period, 56% of episodic CH patients in remission and 71% of migraineurs. No significant correlations were found between the SPECT images and the duration of the disease, of cluster periods or of remissions. Increased parasellar activity on Gallium SPECT is thus not specific for CH, nor for the active period of episodic CH. The method lacks reliability for investigation of putative cavernous sinus inflammation. [less ▲]

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See detailComparison of Cardiac Function in Double-Muscled Calves and in Calves with Conventional Muscular Conformation
Amory, Hélène ULg; Desmecht, Daniel ULg; Linden, Annick ULg et al

in American Journal of Veterinary Research (1994), 55(4), 561-6

During growth, central venous, right ventricular, pulmonary arterial, pulmonary capillary wedge, and systemic arterial pressures, heart rate, and cardiac output were repeatedly measured in 41 Friesian ... [more ▼]

During growth, central venous, right ventricular, pulmonary arterial, pulmonary capillary wedge, and systemic arterial pressures, heart rate, and cardiac output were repeatedly measured in 41 Friesian calves, considered as having conventional muscular conformation, and in 19 Belgian White and Blue double-muscled calves. A total of 123 and 70 recordings were collected in conventional and double-muscled calves, respectively. These circulatory indices were calculated: stroke volume, cardiac and stroke indices, pulmonary and systemic pulse pressures, pulmonary and systemic vascular resistance indices, and right and left ventricular work indices. Results indicated that systemic arterial and pulse pressures, as well as cardiac output, stroke volume, cardiac and stroke indices, and right and left ventricular work indices were significantly (P < or = 0.05 to 0.001) lower but, in contrast, pulmonary and systemic vascular resistance indices were significantly (P < or = 0.001) higher in double-muscled than in conventional calves. Right-sided vascular pressures and heart rate were similar in the 2 groups. These results indicated that global cardiac performance may be considerably poorer in double-muscled calves. Diminished cardiac performance of double-muscled calves appears to be related neither to relative bradycardia nor to reduced ventricular preload. The potential role of increased ventricular afterload or of reduced myocardial contractility in double-muscled cattle should be determined by direct measurements. [less ▲]

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See detailLa prévention de l'ostéoporose postménopausique en cas de contre-indications des œstrogènes
Reginster, Jean-Yves ULg

in Revue Médicale de Liège (1994), 49(4), 194-8

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See detailA new family of "ligated" anionic initiators for the "living" polymerization of (meth)acrylic esters
Bayard, Philippe; Jérôme, Robert ULg; Teyssié, Philippe et al

in Polymer Bulletin (1994), 32(4), 381-385

A new family of ligands (dual sigma - pi ligand), i.e. chelating lithium alkoxides, Li-O-(CH2-CH2O)nCH3, is shown to be very effective in promoting the living anionic Polymerization of methacrylic and ... [more ▼]

A new family of ligands (dual sigma - pi ligand), i.e. chelating lithium alkoxides, Li-O-(CH2-CH2O)nCH3, is shown to be very effective in promoting the living anionic Polymerization of methacrylic and acrylic esters, including a number of primary alkyl acrylates such as n-butyl acrylate and 2-ethylhexylacrylate. [less ▲]

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See detailFlow cytometry in prostate cancer: Histological and clinical correlation
de Leval, Laurence ULg; Waltregny, David ULg; Greimers, Roland ULg et al

in Acta Urologica Belgica (1994), 62(1), 37-43

DNA histograms were obtained by flow cytometry for 39 human prostate carcinomas (27 total prostatectomy specimens, 5 biopsies and 7 transuretral resections). The study was performed on formalin-fixed and ... [more ▼]

DNA histograms were obtained by flow cytometry for 39 human prostate carcinomas (27 total prostatectomy specimens, 5 biopsies and 7 transuretral resections). The study was performed on formalin-fixed and paraffin-embedded material. In this report, ploidy index did not seem to be a good marker of prognosis as no significant variation in ploidy was found neither among the different stages nor among the different Gleason categories. Proliferative index of the tumors seemed to be a more sensitive parameter; a significant relation was observed between proliferative index and stage of the tumor. The authors discuss these results under the light of previously reported observations. [less ▲]

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See detailPolitique et pouvoir
Verjans, Pierre ULg

in Revue Nouvelle (1994)

Las travaux publiés à Liège illustrent une nouvelle tendance à l'intérêt pour les études sur les migrations.

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See detailSex Difference in the Neurotensin-Immunoreactive Cell Populations of the Preoptic Area in Quail (Coturnix Japonica)
Absil, Philippe ULg; Balthazart, Jacques ULg

in Cell & Tissue Research (1994), 276(1), 99-116

The distribution of neurotensin-immunoreactive cells and fibers was analyzed by immunocytochemistry in the forebrain of male and female Japanese quail (Coturnix japonica) by using an antibody directed ... [more ▼]

The distribution of neurotensin-immunoreactive cells and fibers was analyzed by immunocytochemistry in the forebrain of male and female Japanese quail (Coturnix japonica) by using an antibody directed against the C-terminal part of the molecule. [less ▲]

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See detailExpression and Induction of Drug-Metabolizing Enzymes in Cultured Fetal Rat Hepatocytes
Kremers, Pierre ULg; Roelandt, L.; Stouvenakers, Nadine ULg et al

in Cell Biology and Toxicology (1994), 10(2), 117-25

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations ... [more ▼]

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations. Hepatocytes cultured up to 3 weeks in the presence of dexamethasone and phenobarbital still expressed some drug-metabolizing enzyme activities (e.g., ECOD). The enzymatic activities were measured both directly on monolayers during culture and on the corresponding harvested and homogenized cells. The results correlate perfectly with each other. The 'on cell' procedure allows us to repeat the assay or to measure several activities on the same cells at different time intervals. The presence of dexamethasone in the culture medium allows the expression and the induction of several cytochrome P450 isoenzymes, namely, those hydroxylating testosterone. This makes the model particularly attractive for induction experiments as well as for metabolic or toxicological studies needing longer treatments. [less ▲]

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See detailDiagnostic: L'apport du laboratoire
Nikkels, Arjen ULg; Rentier, Bernard ULg

in Virologics (1994), 1

Chez le sujet immunodéprimé (SIDA, greffe, cancer, médicaments), la femme enceinte et le nouveau-né, la gravité potentielle des infections herpétiques exige l'instauration rapide d'un traitement efficace ... [more ▼]

Chez le sujet immunodéprimé (SIDA, greffe, cancer, médicaments), la femme enceinte et le nouveau-né, la gravité potentielle des infections herpétiques exige l'instauration rapide d'un traitement efficace. Etant donné que, chez ces sujets, des formes atypiques rendent le tableau clinique souvent déroutant, il est indispensable de pouvoir disposer de techniques diagnostiques rapides et précises. [less ▲]

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See detailVers une Politique Européenne de l'Immigration, Research Paper n° 3
Martiniello, Marco ULg

in The Philip Morris Institute for Public Policy (1994)

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See detailCrime Prevention in Belgium: Towards "integrated prevention"
Kellens, Georges ULg; Comeron, Manuel ULg

in International Criminal Police Review (1994), (447), 7-15

Tragic events can provide the opportunity for an overall rethink of crime prevention. Recent history in belgium has led to crime prevention being recentred at local level for that is where it can be ... [more ▼]

Tragic events can provide the opportunity for an overall rethink of crime prevention. Recent history in belgium has led to crime prevention being recentred at local level for that is where it can be deployed most effectively. [less ▲]

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See detailPossible pathogenic role of IgE in Henoch-Schonlein purpura
Davin, J. C.; Pierard, Gérald ULg; Dechenne, Charles ULg et al

in Pediatric Nephrology : Journal of the International Pediatric Nephrology Association (1994), 8(2), 169-171

The incidences of clinical and biological markers of atopy were investigated in 16 children with IgA nephropathy (IgAN) (group A) and in 22 with Henoch-Schonlein purpura nephritis (HSPN) (group B). The ... [more ▼]

The incidences of clinical and biological markers of atopy were investigated in 16 children with IgA nephropathy (IgAN) (group A) and in 22 with Henoch-Schonlein purpura nephritis (HSPN) (group B). The incidence of increased plasma IgE levels according to age-matched normal values was significantly higher in group B (17/22, 77%) than in group A (7/16, 44%) (P < 0.05). Although not significant, the incidences of positive RAST tests and of a history of typical atopic symptoms were also higher in group B [10/22 (45%) and 11/22 (50%), respectively] than in group A [4/16 (25%) and 5/16 (31%), respectively]. Moreover, IgE deposits were demonstrated by a peroxidase/anti-peroxidase method on cutaneous Langerhans and mast cells in 4 of 6 patients with HSPN. Thus immunoallergy might account, in some cases, for the cutaneous, intestinal and pulmonary signs observed in HSPN, but not in IgAN. We postulate stimulation of IgE-sensitized mast cells by specific antigens in the presence of IgA circulating immune complexes (CIC), release of vasoactive substances, increased capillary permeability and perivascular deposition of IgA CIC. [less ▲]

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See detailSelective localization of carbon black in immiscible polymer blends : a useful tool to design electrical conductive composites
Gubbels, Frédéric; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(7), 1972-1974

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See detailBinding Site-Shaped Repeated Sequences of Bacterial Wall Peptidoglycan Hydrolases
Ghuysen, Jean-Marie ULg; Lamotte-Brasseur, Josette; Joris, Bernard ULg et al

in FEBS Letters (1994), 342(1), 23-28

The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of ... [more ▼]

The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of Bacillus licheniformis were previously reported to have similarities with the amino acid sequence of the non-catalytic N-terminal module of the Streptomyces albus G Zn DD-peptidase. This peptidase is a bipartite protein of known three-dimensional structure. Its non-catalytic N-terminal module possesses, exposed at the surface, an elongated crevice which is defined by a loop-helix-loop-helix motif that consists of two repeats, each 16 amino acid residues long, connected by a heptapeptide and whose design is compatible with its possible functioning as a substrate recognition and binding site. Amino acid alignments suggest that cavities nearly identical in shape to that present in the non-catalytic module of the S. albus peptidase, are borne by the C-terminal regions of the CwlA amidase (in one copy), the lysozyme and the ORFL3 and CwlL amidases (in two copies). Since a common feature of the five enzymes is their substrate, the bacterial cell wall peptidoglycan, we interpret the striking similarity of their non-catalytic N- or C-terminal modules to suggest that these modules are involved in the binding of these exocellular enzymes to their insoluble wall substrate. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 13. Carbon-13 NMR characterization of the mixed complexation of methyl α-lithioisobutyrate by lithium tert-butyoxide in tetrahydrofuran
Wang, Jin-Shan; Jérôme, Robert ULg; Warin, Roger et al

in Macromolecules (1994), 27(24), 1691-1696

The mixed complexation of methyl alpha-lithioisobutyrate(MIBLi)(a monomeric model structurally corresponding to the living end of PMMA-Li+) with lithium tert-butoxide (LiOtBu) in THF has been studied for ... [more ▼]

The mixed complexation of methyl alpha-lithioisobutyrate(MIBLi)(a monomeric model structurally corresponding to the living end of PMMA-Li+) with lithium tert-butoxide (LiOtBu) in THF has been studied for the first time by using C-13 NMR spectroscopy. It is shown that LiOtBu, like LiCl, is very effective in coordinating with MIBLi tetramer in THF, incrementally replacing MIBLi with the formation of other tetrameric complexes, MIBxLi4(OtBu)4-x (x = 1-3). Complextion of MIBLi by LiOtBu induces a shielding C-13 shift of the MIBLi C(alpha) resonance, which might suggest that lithium cations associated with the electron-rich ligands, i.e., LiOtBu, can also form weaker bonds with the carbonyl oxygen of MIBLi (i.e., mu-type complexes), thereby decreasing the localization of the negative charge on that latter atom. In comparison with LiCl, one of the most significant features is that only one single type of species results from mixed complexation of MIBLi and LiCl at LiCl/MIBLi = 1, 2, and greater-than-or-equal-to 3, respectively, at low temperature, whereas several LiOtBu-complexed species may coexist, whatever the LiOtBu/MIBLi ratio; furthermore, their exchange is found to be very slow at a temperature as high as 0-degrees-C. Tentatively, this striking feature may explain nicely the large difference in molecular weight distribution of poly(meth)acrylates anionically prepared in the presence of LiOtBu and LiCl, respectively. [less ▲]

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See detailLe médecin de famille et l'hôpital de jour: complémentarité de soins
Triffaux, Jean-Marc ULg; Halkin, Stéphane ULg; Bertrand, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(3), 133-40

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See detailManifestations cutanées des maladies pulmonaires
Corhay, Jean-Louis ULg; Bury, Thierry ULg; Louis, Renaud ULg et al

in Revue Médicale de Liège (1994), 49(3), 141-52

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See detailPotentiation of Histamine Release from Human Leucocytes by Paf
Louis, Renaud ULg; Bury, Thierry ULg; Corhay, Jean-Louis ULg et al

in Agents and Actions (1994), 41(1-2), 5-10

Studies on the effects of PAF on histamine release from human leucocytes have yielded conflicting results. We therefore investigated the effects of PAF on leucocytic histamine release (HR) focusing on ... [more ▼]

Studies on the effects of PAF on histamine release from human leucocytes have yielded conflicting results. We therefore investigated the effects of PAF on leucocytic histamine release (HR) focusing on direct as well as on modulating effects. Peripheral blood leucocytes of normal and atopic subjects were incubated with PAF, anti-IgE and FMP for 30 min at 37 degrees C, and histamine was measured fluorometrically. Unlike anti-IgE (1/2000) and FMP (10(-5) M) which caused histamine release (HR) of 34 +/- 7% and 31 +/- 8%, respectively, PAF by itself (10(-11)-10(-5) M) failed to induce any significant HR from human leucocytes (< 3%) in normal (n = 14) and atopic subjects (n = 6). Nevertheless, in normals as well as atopics, PAF, but not lyso-PAF, enhanced anti-IgE (1/2000) and FMP (10(-5) M)-induced HR in a concentration-related manner. Maximal potentiation of histamine release caused by FMP and anti-IgE was achieved with PAF (10(-7)) (mean +/- SEM: 26 +/- 5%, n = 5, p < 0.01) and PAF (10(-5)) (mean +/- SEM: 20 +/- 7%, n = 7, p < 0.05), respectively. This potentiation was suppressed by WEB2086 (10(-5) M), a specific PAF antagonist. The time course of the enhancing effect produced by PAF was dependent on the type of secretagogue. The enhancement was nearly maximal when PAF and FMP were added simultaneously to the leucocytes, whereas a preincubation of 20 min with PAF was required to get maximal enhancement with anti-IgE. The enhancing activity of PAF on HR induced by both anti-IgE and FMP was reversed by washing the cells after preincubation. While PAF enhancement of FMP-induced HR persisted on mononuclear cell fraction containing basophils, that of anti-IgE-induced HR was considerably reduced under these conditions.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailL'invasion cancéreuse mammaire: le rôle clé des cellules normales des tissus hôtes
Foidart, Jean-Michel ULg; Polette, M.; Birembaut, P. et al

in Bulletin de l'Académie Nationale de Médecine (1994), 178(3), 533-42542-4

Tumor progression is influenced by extracellular matrices and by soluble factors or cytokines locally produced by host tissue cells (fibroblasts, immune cells ...). Such factors may also accumulate in ... [more ▼]

Tumor progression is influenced by extracellular matrices and by soluble factors or cytokines locally produced by host tissue cells (fibroblasts, immune cells ...). Such factors may also accumulate in close association with some extracellular matrix molecules in the tumor. They may also be unmasked during breaking down of extracellular matrices. The most insidious aspect of tumors is their propensity to locally invade normal tissues of the host and to form secondary foci in organs at distant sites from the primary tumor called metastases. During this process, invasive cells come into contact with host tissue cells such as fibroblasts, endothelial cells, macrophages, lymphocytes. These cells are not the passive witnesses of the metastatic cascade but actively participate to the malignant invasion. Through soluble messages (cytokines) and through insoluble molecules of the extracellular matrix, neoplastic and normal cells mutually modulate their activities. Cancer cells regulate the biosynthetic activities of fibroblasts and alter in this way the scaffold of the tumor. Reciprocally, host cells secrete extracellular matrix proteins and cytokines which influence the growth and activities of tumor cells. They also produce at the periphery of tumor cells proteolytic enzymes which promote host tissue destruction and cancerous cells migration. Among these enzymes, matrix metalloproteinases appear to play a key role during invasion and metastasis. Tumors represent thus a complex ecosystem. Tumor cells interact with several components of the extracellular matrix and with host cells (immune cells, fibroblasts, endothelial cells). Such multiple cell-cell and cell-matrix interactions condition angiogenesis, tumor growth, destruction of host tissues, local migration of cancer cells and their metastatic dissemination. It is probable that a precise knowledge of the genes which are selectively activated in tumors under the influence of the host cells or of the tumor cells will allow to define new therapeutic strategies. These treatments will aim not at destroying the metastatic neoplastic cells but at preventing their growth by interfering with their microenvironment. [less ▲]

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See detailL'associatif au carrefour des économies formelle et informelle
Defourny, Jacques ULg

in Recherches Sociologiques et Anthropologiques (1994), (1994/3), 109-127

Depuis toujours, la vie associative s’est exprimée à la fois dans des formes officiellement admises par les gouvernants et dans des cadres informels, souvent même clandestins. La reconnaissance du statut ... [more ▼]

Depuis toujours, la vie associative s’est exprimée à la fois dans des formes officiellement admises par les gouvernants et dans des cadres informels, souvent même clandestins. La reconnaissance du statut juridique d’association sans but lucratif (A.S.B.L.) en 1921 a ouvert la voie à un développement considérable des structures associatives en Belgique, mais une part substantielle du phénomème associatif continue à se manifester en dehors de ce statut. Quant au bénévolat, très important dans de nombreuses associations, il renforce encore l’ancrage de l’associatif dans l’économie informelle. La coexistence de dimensions formelles et informelles ainsi que la pauvreté des statistiques officielles expliquent pour une bonne part le peu d’intérêt des économistes pour les associations. Ces dernières années cependant, l’analyse économique des associations a progressé et quelques enquêtes sont venues compléter les élémentaires recensements officiels. L’inventaire des données actuellement disponibles en Belgique montre toutefois l’ampleur du chemin qui reste à parcourir. [less ▲]

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See detailAromatase-Immunoreactive Cells in the Quail Brain: Effects of Testosterone and Sex Dimorphism
Foidart, Agnès ULg; de Clerck, A.; Harada, N. et al

in Physiology & Behavior (1994), 55(3), 453-64

We previously demonstrated that testosterone (T) increases aromatase activity (AA) and that AA is sexually dimorphic (males > females) in the quail preoptic area (POA). The precise anatomical localization ... [more ▼]

We previously demonstrated that testosterone (T) increases aromatase activity (AA) and that AA is sexually dimorphic (males > females) in the quail preoptic area (POA). The precise anatomical localization of these effects is, however, impossible to obtain by biochemical assays even when samples are dissected by the Palkovits punch technique. We were recently able to set up an immunocytochemical (ICC) procedure that permits visualization of aromatase-immunoreactive (ARO-ir) cells in the quail brain. This showed that the ARO-ir cells of the quail POA actually outline the sexually dimorphic medial preoptic nucleus (POM). This ICC technique was used here to analyze the sex dimorphism of the quail preoptic aromatase and the localization of T effects on ARO-ir cells. In Experiment 1, the number of ARO-ir cells was counted in one section every 100 microns throughout the rostral to caudal extent of the POM of castrated birds that had been treated with increasing doses of T (5, 10, or 20 mm long Silastic implants). These T-treatments produced a dose-related increase in the sexual behavior of the birds and they increased the number of ARO-ir cells in POM, in the septal regions, and in the bed nucleus of the stria terminalis (BNST). The effect had a particularly large amplitude in the part of the POM located under the anterior commissure (AC). In Experiment 2, the same procedure was used to reanalyze the sex difference of the preoptic aromatase system. This showed that the POM of adult males contains more stained cells than the POM of females but only in a restricted region located just under and rostral to the AC. No significant sex difference was observed in the septum or in the BNST. In Experiment 3, the number of ARO-ir cells was determined in the POM of males and females that had been gonadectomized and treated with a same dose of T (40 mm implants). No sex difference in the number of ARO-ir cells could be detected in these conditions. This suggests that the sex difference in AA that had been previously observed in T-treated birds results either from a difference in aromatase concentration or activity in a similar number of positive cells or from a difference in the number of ARO-ir cells that is very discrete from the anatomical point of view.(ABSTRACT TRUNCATED AT 400 WORDS) [less ▲]

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See detailThe Perturbed Proximal Point Algorithm and Some of its Applications
Tossings, Patricia ULg

in Applied Mathematics & Optimization (1994), 29(2), 125-159

Following the works of R.T. Rockafellar, to search for a zero of a maximal monotone operator, and of B. Lemaire, to solve convex optimization problems, we present a perturbed version of the proximal point ... [more ▼]

Following the works of R.T. Rockafellar, to search for a zero of a maximal monotone operator, and of B. Lemaire, to solve convex optimization problems, we present a perturbed version of the proximal point algorithm. We apply this new algorithm to convex optimization and to variational inclusions or, more particularly, to variational inequalities. [less ▲]

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See detailPorc et reduction des rejets azotes.
Leterme, Pascal; Thewis, André ULg

in Agricontact (1994), (259), 3-4

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See detailGonadotropin Releasing Hormone Inhibitory Autofeedback by Subproducts Antagonist at N-Methyl-D-Aspartate Receptors: A Model of Autocrine Regulation of Peptide Secretion
Bourguignon, Jean-Pierre ULg; Alvarez Gonzalez, Maria-Luz ULg; Gerard, Arlette ULg et al

in Endocrinology (1994), 134(3), 1589-92

The secretion of Gonadotropin-releasing Hormone (GnRH) involves activation of N-methyl-D-aspartate (NMDA) receptors. Here, we show that pulsatile GnRH secretion from hypothalamic explants is suppressed by ... [more ▼]

The secretion of Gonadotropin-releasing Hormone (GnRH) involves activation of N-methyl-D-aspartate (NMDA) receptors. Here, we show that pulsatile GnRH secretion from hypothalamic explants is suppressed by 1-5GnRH, an endogenous breakdown product of GnRH, while 2-10GnRH has no effect. GnRH secretion evoked by NMDA is selectively inhibited by 1-5GnRH and this effect is similar to that of AP-5, a competitive antagonist at NMDA receptors. In addition, 1-5GnRH accounts for a dose-related inhibition of tritiated glutamate binding to hypothalamic membrane preparations. Using GnRH secretion as a model of NMDA-receptor controlled system, the effect of different peptides has been studied. Growth Hormone Releasing Factor (GRF), Insulin-like Growth Factor-I (IGF-I) and Proinsulin result in inhibition of GnRH secretion. Bioactive subproducts of those peptides (1-29GRF, 4-701GF-I and insulin) do not show any effect, suggesting that their classical receptors are not involved. In contrast, GnRH secretion is inhibited by other subproducts (1-37GRF, 1-31GF-I and C-peptide) all terminating in a glutamate residue. These subproducts selectively suppress the NMDA-evoked secretion of GnRH. Protease inhibitors prevent the inhibitory effects of IGF-I on GnRH secretion. This, breakdown products of different peptide hormones are possible endogenous antagonists at NMDA receptors. This effect could account for an autocrine or paracrine limitation of NMDA-receptor-mediated secretion of peptides. [less ▲]

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See detailIn Vivo 31p Nuclear Magnetic Resonance Spectroscopy of Skeletal Muscle Energetics in Endotoxemic Rats: A Prospective, Randomized Study
Gilles, R. J.; D'Orio, Vincenzo ULg; Ciancabilla, F. et al

in Critical Care Medicine (1994), 22(3), 499-505

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal ... [more ▼]

OBJECTIVE: To identify possible alterations in the skeletal muscle high-energy phosphate metabolism at the early phase of endotoxic shock in rats. DESIGN: A prospective, randomized study of skeletal muscle energetics in endotoxemic and in control rats, by in vivo 31P nuclear magnetic resonance (NMR) spectroscopy at rest, under regional ischemia, and during reperfusion. SETTING: Biochemical NMR laboratory equipped for surgery and hemodynamic monitoring. SUBJECTS: Wistar rats were randomized to different groups. Eight rats were injected with Escherichia coli endotoxin (15 mg/kg, survival time 19 +/- 4 hrs) intraperitoneally. Seven other rats served as controls. The additional nine rats were studied for the saturation recovery pulse sequence. INTERVENTIONS: In the treatment group, endotoxin was injected 8 hrs before NMR spectroscopy. The right hind limbs were studied under anaesthesia using a surface coil NMR probe. Their high-energy phosphate contents and intracellular pH were determined by 31P NMR spectroscopy. After baseline measurements, an ischemia-reperfusion challenge was imposed on the muscle by transient clamping of the abdominal aorta. Contralateral femoral artery pressure was constantly monitored. MEASUREMENTS AND MAIN RESULTS: During the baseline period, the endotoxin-treated muscles did not show any difference in the distribution of the high-energy phosphate compounds or in intracellular pH, as compared with the controls. Ischemia resulted in a significantly faster decline of the inorganic phosphate/creatine phosphate ratio in the endotoxin-treated rats (1.35 +/- 0.17 vs. 0.51 +/- 0.06 at the end of the 38-min ischemic period). Skeletal muscle acidosis developed earlier and was deeper in the endotoxemic animals (pH: 6.94 +/- 0.02 vs. 7.02 +/- 0.03 at the end of ischemia). During reperfusion, the calculated time constants of recovery of inorganic phosphate to phosphocreatine ratios were identical between groups. CONCLUSIONS: Resting nonischemic muscles of endotoxin-treated rats show no evidence of alterations in high-energy phosphate metabolism. However, under ischemic conditions, high-energy phosphate metabolism deteriorates faster in the skeletal muscles of treated animals. These data support the hypothesis of a greater mismatch during perfusion at very low pressure between residual oxygen availability and oxygen needs in the endotoxin-treated muscle cell. [less ▲]

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See detailQuenching of Merocyanine 540 Triplet State by Nitroxyl Radicals in Liposomal Systems: A Laser Flash Photolysis Study
Hoebeke, Maryse ULg; Enescu, M.; Lindqvist, L.

in Journal of Photochemistry and Photobiology B : Biology (1994), 22(3), 229-33

Laser flash photolysis experiments were undertaken to investigate the interaction between stearic acid nitroxide spin probes and photoexcited merocyanine 540 (MC540) in dimyristoyl-L-alpha ... [more ▼]

Laser flash photolysis experiments were undertaken to investigate the interaction between stearic acid nitroxide spin probes and photoexcited merocyanine 540 (MC540) in dimyristoyl-L-alpha-phosphatidylcholine liposomes (membrane model). The measurements of the paramagnetic signal decay kinetics of four different spin-labelled stearic acids (n-DSA) show that the direct interaction between the dye and the probe is affected by the position of the nitroxyl group along the carbon chain. Laser flash photolysis results reveal a significant decrease in the MC540 triplet lifetime in the presence of n-DSA, the effect depending on the depth at which the nitroxyl moiety is localized in the bilayer. Previous results on the rate of disappearance of the electron spin resonance (ESR) nitroxide signal on continuous photolysis of the same systems do not show the same dependence on the localization of the nitroxyl moiety in the liposome. Although the MC540 triplet state seems to be implicated in the reaction process, the results suggest that ESR and laser flash experiments demonstrate two different kinds of mechanism. [less ▲]

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See detailImmunocytochemical localization of vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the bovine ovary
Hulshof, S. C. J.; Dijkstra, G.; Van der Beek, E. M. et al

in Biology of Reproduction (1994), 50(3), 553-560

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty ... [more ▼]

The distribution of the neuropeptides vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) was studied immunocytochemically in bovine ovaries from 3 mo of gestation up to and including puberty, and from adult cows at three stages of the estrous cycle. The appearance of VIP and NPY immunoreactivity of 4.5-6 mo of gestation coincided with the onset of follicular development. In contrast to NPY, VIP was first found in the cortex. Both VIP and NPY immunoreactivity increased with age. From 9 mo of gestation onwards, VIP and NPY were found around blood vessels and non-vascular smooth muscle cells, in the stroma near preantral follicles, and in the theca externa of antral follicles. In addition, VIP-positive cells were observed exclusively in the granulosa layer of the preovulatory follicle at the time of the LH surge. The distribution of VIP- and NPY-immunoreactive fibers in the ovary may point to an effect of these neuropeptides on various physiological processes, including follicle development and ovarian blood flow. In addition, the presence of VIP-positive cells in the granulosa layer of the preovulatory follicle is indicative of a role for VIP in ovulation. [less ▲]

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See detailSalt-free polyelectrolyte behavior of polystyrene-based telechelic ionomers in a polar solvent. 1. Viscosity and low-angle light scattering studies
Wu, Jhi-Li; Wang, Yu-Min; Hara, Masanori et al

in Macromolecules (1994), 27(5), 1195-1200

Some characteristic behavior, typically observed for salt-free polyelectrolytes in water, was observed for the first time for polystyrene (PS)-based telechelic ionomers, which have ionic groups located ... [more ▼]

Some characteristic behavior, typically observed for salt-free polyelectrolytes in water, was observed for the first time for polystyrene (PS)-based telechelic ionomers, which have ionic groups located only at the chain ends, when dissolved in a polar solvent, dimethylformamide (DMF). The characteristic behavior observed by both viscosity and low-angle light scattering measurements for the telechelic ionomers, especially that of the monofunctional ionomer, demonstrates an important role of intermolecular electrostatic interactions in causing the characteristic behavior of telechelic ionomers. The behavior observed in our experiments can best be described by the following picture: each ionomer molecule with a coil conformation, very close to that of polystyrene of the same molecular weight, is separated from yet strongly interacts with other ionomer molecules; and these strong intermolecular electrostatic interactions cause a marked upturn in reduced viscosity and a reduction in scattered intensity. [less ▲]

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See detailEngineering and Overexpression of Periplasmic Forms of the Penicillin-Binding Protein 3 of Escherichia Coli
Fraipont, Claudine ULg; Adam, Maggy; Nguyen-Disteche, Martine et al

in Biochemical Journal (1994), 298(1), 189-195

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of ... [more ▼]

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of F37-V577 PBP3 and G57-V577 PBP3 respectively into the periplasm. The modified ftsI genes were placed under the control of the fused lpp promoter and lac promoter/operator; expression of the truncated PBP3s was optimized by varying the copy number of the recombinant plasmids and the amount of LacI repressor, and export was facilitated by increasing the SecB content of the producing strain. The periplasmic PBP3s (yield 8 mg/l of culture) were purified to 70% protein homogeneity. They require the presence of 0.25 M NaCl to remain soluble. Like the membrane-bound PBP3, they undergo processing by elimination of the C-terminal decapeptide I578-S588, they bind penicillin in a 1:1 molar ratio and they catalyse hydrolysis and aminolysis of acyclic thioesters that are analogues of penicillin. The membrane-anchor-free PBP3s have ragged N-termini. The G57-V577 PBP3, however, is less prone to proteolytic degradation than the F37-V577 PBP3. [less ▲]

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See detailPyridobenzoxazepine and Pyridobenzothiazepine Derivatives as Potential Central Nervous System Agents: Synthesis and Neurochemical Study
Liégeois, Jean-François ULg; Rogister, F. A.; Bruhwyler, J. et al

in Journal of Medicinal Chemistry (1994), 37(4), 519-25

In order to characterize the pharmacological profile of the different chemical classes of pyridobenzazepine derivatives, a series of N-methylpiperazinopyrido[1,4]- and -[1,5]- benzoxa- and benzothiazepine ... [more ▼]

In order to characterize the pharmacological profile of the different chemical classes of pyridobenzazepine derivatives, a series of N-methylpiperazinopyrido[1,4]- and -[1,5]- benzoxa- and benzothiazepine derivatives were prepared. The affinities for D2, D1, 5-HT2, and cholinergic (M) receptors were measured. In comparison to dibenzazepine reference compounds, a strong decrease of the affinities was observed, less pronounced, however, for the substituted analogues. Oxazepine and thiazepine analogues like clozapine (except 8-chloro-6-(4-methylpiperazin-1-yl)-pyrido[2,3-b][1,4]benzoxazepin e (9) and 8-chloro-6-(4-methylpiperazin-1-yl)pyrido[2,3-b][1,4]- benzothiazepine (11)) were found to be inactive against apomorphine stereotypies. In the open-field test in rats, different molecules showed a high disinhibitory activity as observed with anxiolytic drugs. Moreover, 8-chloro-5-(4-methylpiperazin-1-yl)pyrido[2,3-b][1,5]benzoxazepine (14) presented a clozapine-like profile that was confirmed in the behavioral model in dogs and showed most of the behavioral characteristics described for antipsychotic drugs. Its neurochemical profile, in particular the 5-HT2/D2 ratio, was also compatible with atypical antipsychotic activity. [less ▲]

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See detailFIELD OBSERVATIONS ON A MATING ATTEMPT OF A SPAWNING GRAYLING, THYMALLUS-THYMALLUS WITH A FEEDING BARBEL, BARBUS-BARBUS
Poncin, Pascal ULg

in Journal of Fish Biology (1994), 45(5), 904-906

During the spawning period of the grayling, one male was sexually stimulated by a feeding barbel and consequently exhibited the behavioural pattern of 'approach', 'quivering' and 'dorsal fin clasping'.

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See detailCoordinate Enhancement of Gelatinase a Mrna and Activity Levels in Human Fibroblasts in Response to Breast-Adenocarcinoma Cells
Noël, Agnès ULg; Polette, M.; Lewalle, J. M. et al

in International Journal of Cancer = Journal International du Cancer (1994), 56(3), 331-6

Gelatinases/type-IV collagenases are metalloproteinases involved in some carcinoma invasion and metastatic processes. The exact cellular source of the 72-kDa gelatinase A is controversial. We have ... [more ▼]

Gelatinases/type-IV collagenases are metalloproteinases involved in some carcinoma invasion and metastatic processes. The exact cellular source of the 72-kDa gelatinase A is controversial. We have analyzed the expression of mRNA coding for gelatinase A in vivo by in situ hybridization on breast-cancer tissues. The mRNA for gelatinase A was present in fibroblasts. We have therefore evaluated the gelatinase-A activity in vitro, in co-cultures of different breast adenocarcinoma cell lines and human fibroblasts. In monoculture, none of the tumor cells tested produced detectable amounts of gelatinase A. The gelatinase-A activity was enhanced in cultures of fibroblasts maintained in the presence of MDA-MB 231 or SKBR3 cells, or their conditioned medium. This increased enzymatic activity was evidenced both in the culture medium and in the membrane fraction and was paralleled by enhancement of the steady-state levels of mRNA. These results are an in vitro demonstration of a regulation of fibroblasts gelatinase-A production by soluble factors secreted by breast-tumor cells. [less ▲]

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See detailEffects of Alpha-Hederin, a Saponin Extracted from Hedera Helix, on Cells Cultured in Vitro
Danloy, S.; Quetin-Leclercq, J.; Coucke, P. et al

in Planta Medica (1994), 60(1), 45-9

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in ... [more ▼]

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in vitro. Our results indicate that, in a serum-free medium, alpha-hederin is cytotoxic and inhibits proliferation in both cell lines at rather low concentrations (< 5 micrograms/ml) after only 8 hours of treatment. Its cytotoxicity decreases in the presence of serum in which BSA seems to be able to bind the saponin. alpha-Hederin also induces vacuolization of the cytoplasm and membrane alterations leading to cell death. [less ▲]

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See detailSelf-Treatment of Acute Migraine with Subcutaneous Sumatriptan Using an Auto-Injector Device: Comparison with Customary Treatment in an Open, Longitudinal Study
Schoenen, Jean ULg; Bulcke, J.; Caekebeke, Jo et al

in Cephalalgia : An International Journal of Headache (1994), 14(1), 55-63

In a multicenter open longitudinal clinical trial where 479 patients suffering from migraine with or without aura were recruited, patients treated at home one to three migraine attacks with their ... [more ▼]

In a multicenter open longitudinal clinical trial where 479 patients suffering from migraine with or without aura were recruited, patients treated at home one to three migraine attacks with their customary treatment, and subsequently, over a 3-month period, one to three migraine attacks with 6 mg sumatriptan sc using an autoinjector. The headache response to customary treatment was 19% at 1 h and 30.5% at 2 h, and was not significantly different when only attacks treated "adequately" according to accepted treatment recommendations were considered: 16% at 1 h and 35% at 2 h. In contrast, 69% and 82% of patients treated with 6 mg sumatriptan sc had mild headache or no headache at 1 and 2 h respectively, regardless of migraine type or duration of symptoms prior to treatment. Other migraine symptoms (nausea, vomiting, photo- and phonophobia) were effectively treated with sumatriptan. Recurrence of migraine was observed in 31% of patients and was well controlled by a second injection of sumatriptan. It is concluded that 6 mg sumatriptan sc, self-administered using an autoinjector, is well tolerated and more effective than most currently used acute treatments for migraine in a population of severely affected patients consulting a neurologist. [less ▲]

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See detailLes traitements physiques des eaux dures.
Marcoen, Jean Marie ULg; Lédion, J.

in Tribune de l'Eau (La) (1994), 47

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See detailCalcul de l'équilibre calcocarbonique des eaux sur calculatrice de poche.
Wuidar, A.; Marcoen, Jean Marie ULg

in Tribune de l'Eau (La) (1994), 47(1), 11-13

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See detailHomeobox Genes: Potential Candidates for the Transcriptional Control of the Transformed and Invasive Phenotype
Castronovo, Vincenzo ULg; Kusaka, Masami; Chariot, Alain ULg et al

in Biochemical Pharmacology (1994), 47(1), 137-43

The transformation of a cell and the acquisition of the invasive and metastatic phenotype result from the activation of a group of complex cellular processes rather than from the effect of a single gene ... [more ▼]

The transformation of a cell and the acquisition of the invasive and metastatic phenotype result from the activation of a group of complex cellular processes rather than from the effect of a single gene product. It is likely that the coordination of the multiple genes involved in malignancy is under the control of a few genes that act as master genes or orchestrator genes. The latter probably code for transcription factors that control the genetic program for tumor invasion and metastasis. Homeobox genes are a family of transcription factors that contain a 183 bp highly conserved nucleotide sequence coding for a 61 amino acid domain that binds specifically to DNA. First discovered in Drosophila as genes controlling segmentation and segment identity, homeobox genes have since been identified in many other species including nematodes, frog, mouse and human. There is strong support for the suggestion that homeobox genes play a key role in development and differentiation. In humans, there are 38 homeobox genes organized in four clusters that are localized on chromosomes 2, 7, 12 and 17. The specific functions of each of these genes are generally unknown. Alterations in expression of several homeobox genes have been reported in a variety of malignant lesions, suggesting that they could play a role in the development of cancer. Using reverse transcriptase reaction coupled with polymerase chain reaction and degenerate oligonucleotides corresponding to the 5' and 3' ends of the highly conserved homeodomain, we amplified 130 bp cDNA fragments from the human breast cancer cell line MCF7 that were subsequently cloned into pBluescript vector. Sequencing of the clones, resulted in the identification of the homeodomains of four different human homeobox genes: HOXB6, HOXA1, HOXA10 and HOXC6. Further studies should determine the specific role of these four homeobox genes in the development and progression of human breast cancer and potentially determine if they might be good targets for gene therapy. [less ▲]

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See detailThe Nf-Kappa B Transcription Factor and Cancer: High Expression of Nf-Kappa B- and I Kappa B-Related Proteins in Tumor Cell Lines
Bours, Vincent ULg; Dejardin, Emmanuel ULg; Goujon-Letawe, F. et al

in Biochemical Pharmacology (1994), 47(1), 145-9

NF-kappa B is a pleiotropic transcription factor which controls the expression of many genes and viruses. To date, there is good evidence, but no definitive proof, for its role in tumor formation and ... [more ▼]

NF-kappa B is a pleiotropic transcription factor which controls the expression of many genes and viruses. To date, there is good evidence, but no definitive proof, for its role in tumor formation and development of metastasis. To investigate the possibility that members of the NF-kappa B family could participate in the molecular control of the transformed and invasive phenotype, we examined the expression of these proteins in a variety of human tumor cell lines. The expression of p50, p65, p52 and I kappa B was quantified at the protein level using western immunoblot and mobility shift assay and at the RNA level by northern blot. We observed high expression of the NF-kappa B inhibitor I kappa B in the ovarian carcinoma cell line OVCAR-3 together with constitutive nuclear NF-kappa B activity. We also studied the colon carcinoma cell line HT-29 and its metastatic counterpart HTM-29 and we observed specific expression of the p52 NF-kappa B-related protein in the metastatic cells. Our data confirm that NF-kappa B could be involved in the genesis of a variety of cancers including solid tumors and provide us with interesting models to explore the exact role of these transcription factors in cancer. [less ▲]

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See detailFurther observational evidence that MG J0414+0534 is a gravitational mirage
Angonin-Willaime, M.-C.; Vanderriest, C.; Hammer, F. et al

in Astronomy and Astrophysics (1994), 281

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the ... [more ▼]

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the (extremely red and almost featureless) spectra of the 2 brightest components are very similar. These are strong indications that the system results from multiple gravitational imaging of a single source, but the nature of this source is not yet clear. It could be the nucleus of a low metallicity galaxy at a high redshift or a new type of object. Significant differences are observed between the flux ratios of the images at radio and optical wavelenghts. The most likely explanation for this effect is a differential amplification of the image pair A[SUB]1[/SUB]-A[SUB]2[/SUB] because of the large magnification gradient near a caustic. [less ▲]

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See detailEvidence for violent ejection of nebulae from massive stars
Hutsemekers, Damien ULg

in Astronomy and Astrophysics (1994), 281(Letters), 81-84

We report the results of a systematic search for nebulae around Luminous Blue Variable LBVs) and the discovery of a strong correlation between the mass of the nebulae and the luminosity of the central ... [more ▼]

We report the results of a systematic search for nebulae around Luminous Blue Variable LBVs) and the discovery of a strong correlation between the mass of the nebulae and the luminosity of the central stars. This correlation holds for both the dust and ionized gas masses of the nebulae. The existence of a 'nebular mass-stellar luminosity' relation and the fact that not all LBVs are presently associated with a nebula give evidence against a continuous mass-loss mechanism for the formation of these nebulae. Further, the good agreement found between the observed relation and predictions by Maeder (1989), suggests that all these nebulae may be due to a violent ejection of matter caused by an instability of structural origin. [less ▲]

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See detailHypnose, hypnosédation. Conceptions actuelles et leurs applications en chirurgie plastique
Faymonville, Marie-Elisabeth ULg; Fissette, Jean ULg; Mambourg, P. H. et al

in Revue Médicale de Liège (1994), 49(1), 13-22

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See detailLe pied diabétique: etiopathogénie, prévention et traitement
Van Damme, Hendrik ULg; Paquet, Philippe ULg; Maertens de Noordhout, B. et al

in Revue Médicale de Liège (1994), 49(1), 1-13

-Le pied diabétique est la conséquence des altérations dégénératives du système vasculonerveux observées dans un diabète de longue durée. La neuropathie diabétique est le facteur essentiel dans la plupart ... [more ▼]

-Le pied diabétique est la conséquence des altérations dégénératives du système vasculonerveux observées dans un diabète de longue durée. La neuropathie diabétique est le facteur essentiel dans la plupart des cas, responsable d'hypoalgésies, microtraumatismes et ulcérations, déformation du pied (amyotrophie), et d'une eutosympethectomie (peau sèche, fissurée). Une macroangiopathie (médiacalcinose, occlusions artérielles périphériques) n'est retrouvée que dans 30 % des cas. Une microangiopathie compromet la trophicité des tissus et ralentit leur cicatrisation. Enfin, tout diabétique présente une susceptibilité élevée aux infections. Cette multitude de facteurs en cause impose des mesures de prévention. Un équilibre du profil glycémique retardera les atteintes vasculo-nerveuses. L'hygiène du pied consistera en bains de pieds, soins d'ongles et d'hyperkératoses, chaussures adaptées. En cas de troubles trophiques, une décharge d'appui sers nécessaire. Une désinfection rigoureuse, associée à une antibiothérapie (après prélèvement, si possible) aidera à éviter l'évolution vers l'abcès profond. La moindre collection sera drainée, après excision large des tissus nécrotiques. Les nécroses sèches (talons, orteils) traduisent souvent une artériopethie, pour laquelle un geste de revascularisation (protondoolestie; pontage distal) pourra être pris en considération. Parfois, l'état septique du patient, ou l'étendue de la gangrène, imposera une amputation. L'approche du pied diabétique doit toujours être multidisciplinaire (diabétologue, dermatologue, orthésiste, orthopédiste, chirurgien vasculaire), et doit commencer par des mesures préventives. [less ▲]

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See detailTransplantations de tumeurs malignes insoupçonnées lors de greffes d'organes
Detry, Olivier ULg; Detroz, Bernard ULg; D'Silva, M. et al

in Revue Médicale de Liège (1994), 49(1), 23-31

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See detailPhotometric variations of Ap stars
Manfroid, Jean ULg; Renson, Pierre ULg

in Astronomy and Astrophysics (1994), 281

The photometric variations of seven Ap stars have been analyzed from observations mainly obtained in the framework of the Long-Term Photometry of Variables project at European Southern Observatory (ESO ... [more ▼]

The photometric variations of seven Ap stars have been analyzed from observations mainly obtained in the framework of the Long-Term Photometry of Variables project at European Southern Observatory (ESO). New values for the periods and detailed light curves in the uvby system are provided. Alpha Scl is shown to display anomalous variations that set it apart from the other stars. [less ▲]

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See detailLong-Term (3 Years) Prevention of Trabecular Postmenopausal Bone Loss with Low-Dose Intermittent Nasal Salmon Calcitonin
Reginster, Jean-Yves ULg; Denis, D.; Deroisy, Rita ULg et al

in Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research (1994), 9(1), 69-73

The long-term effect of intermittent low-dose nasal salmon calcitonin on trabecular early postmenopausal bone loss was assessed as follow-up to a previously published study. Randomized controlled group ... [more ▼]

The long-term effect of intermittent low-dose nasal salmon calcitonin on trabecular early postmenopausal bone loss was assessed as follow-up to a previously published study. Randomized controlled group comparison was made of 287 healthy women with 6-36 months of natural menopause and no treatment interfering with calcium metabolism at an outpatient clinic for research in bone and cartilage metabolism. The 287 women were randomly allocated to 3 years of treatment with either 500 mg/day, 5 days/week of calcium or the same amount of calcium plus 50 IU/day, 5 days per week of nasal salmon calcitonin. A total of 186 women complied with the study protocol throughout. The main outcome measures were bone mineral density of the lumbar spine (DPA) and biochemical parameters reflecting bone turnover (serum alkaline phosphatases, urinary calcium/creatinine, and hydroxyproline/creatinine ratio). The average changes in bone mineral density after 36 months showed a positive (p < 0.05) outcome (1.8 +/- 5.7%; mean +/- SD) in the group treated with salmon calcitonin and calcium and a significant (p < 0.01) loss (-5.8 +/- 4.8%) in patients receiving calcium alone. The difference between the evolution of the two groups was significantly (p < 0.01) different after 6 months of treatment and remained so until the end of the study. No significant changes were recorded in biochemical parameters reflecting bone turnover. As previously shown during a 1 year follow-up, nasal salmon calcitonin given at low dose and intermittently, in association with calcium, can counteract trabecular postmenopausal bone loss. [less ▲]

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See detailLe point sur la production d’embryons in vitro: limitations et perspectives de la recherche
Thonon, Fabienne; Ectors, Fabien ULg; Delval, Alain et al

in Annales de Médecine Vétérinaire (1994), 138(1), 33-40

Recent progress in gamete biology and early embryo development was responsible for great evolution of reproduction techniques in farm animals. Among these techniques, in vitro production of bovine embryos ... [more ▼]

Recent progress in gamete biology and early embryo development was responsible for great evolution of reproduction techniques in farm animals. Among these techniques, in vitro production of bovine embryos was developed from slaughterhouse ovaries. This method has been used for individual animals. However, large variations in results were observed, due to several factors, including reproductive state and age of the donor. Inspite of this limitation, in vitro production of bovine embryos may be helpful, when slaughtering of valuable cows is recommended eg for brucellosis disease. Researches are purchased in order to increase the number of oocytes obtained per "elite" cow and to improve the ability of in vitro embryos to support freezing. [less ▲]

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See detailEffects of Acute Injection of Methylprednisolone in Man on Immunological and Non-Immunological Histamine Release from Leucocytes and Its Potentiation by Interleukin-3
Louis, Renaud ULg; Bury, Thierry ULg; Corhay, Jean-Louis ULg et al

in Clinical & Experimental Allergy : Journal of the British Society for Allergy & Clinical Immunology (1994), 24(1), 60-5

We investigated the effects of intravenous injection of methylprednisolone (MPR) compared with placebo (saline) on ex vivo leucocytic histamine release in eight healthy volunteers. All subjects received ... [more ▼]

We investigated the effects of intravenous injection of methylprednisolone (MPR) compared with placebo (saline) on ex vivo leucocytic histamine release in eight healthy volunteers. All subjects received in a randomized and a single-blind manner the placebo and MPR, 20 mg and 125 mg, each injection given in 2 week intervals. On each occasion blood samples were taken just before and 24 h after the intravenous injection to determine circulating leucocyte counts and leucocytic histamine release induced by anti-IgE (1/2000) and FMP (Formyl-Methionyl-Phenylalanine) (10(-5) M) and its modulation by IL-3 (2 ng/ml). MPR 20 mg and 125 mg significantly increased circulating leucocyte counts (P < 0.05 and P < 0.001 respectively) but decreased leucocytic histamine content (P < 0.05 and P < 0.001 respectively) by 24 h. Placebo had no effect. As for circulating basophils, after 24 h they were decreased by 125 mg MPR (P < 0.05) but increased by 20 mg (P < 0.05). Anti-IgE-induced HR was significantly inhibited by 125 mg MPR (P < 0.05) but not by 20 mg MPR or by the placebo. In contrast, neither MPR (20 mg and 125 mg) nor placebo significantly reduced FMP-induced HR. The strong potentiation by IL-3 of HR evoked by anti-IgE and FMP at baseline (P < 0.001) persisted 24 h after injection of MPR or placebo (P < 0.001 except P < 0.05 for anti-IgE-induced HR after 125 mg MPR).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLa tribologie, une spécialité liégeoise
Bozet, Jean-Luc ULg

in Athena (1994), 97

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See detailEffects of Apomorphine on Sexual Behavior in Male Quail
Absil, Philippe ULg; Das, S.; Balthazart, Jacques ULg

in Pharmacology, Biochemistry & Behavior (1994), 47(1), 77-88

In the rat, dopamine (DA) facilitates male copulatory behavior. Indirect evidence based largely on neuroanatomical data suggest that in quail DA is also implicated in the control of male reproductive ... [more ▼]

In the rat, dopamine (DA) facilitates male copulatory behavior. Indirect evidence based largely on neuroanatomical data suggest that in quail DA is also implicated in the control of male reproductive behavior but there is no pharmacological evidence to support this conclusion. To test this idea, castrated testosterone (T)-treated male quail were injected with various doses of the dopaminergic agonist apomorphine (APO) in the range 1-10,000 micrograms/kg. The sexual behavior of birds was recorded starting 15 min after APO injection for a duration of 30 min. A dose-dependent inhibition of male reproductive behavior that lasted for the entire duration of the test was observed. In a second experiment, gonadectomized T-treated male Japanese quail were injected daily with APO (0, 10, or 1,000 micrograms/kg) during 8 days. Their sexual interactions with a partner were quantified either 24 h or 15 min after the last injection. No influence of the treatment on copulatory behavior was observed 24 h after the last injection, but a strong inhibition was present when the test was performed 15 min after. To research whether the inhibitory effects of APO were due to a preferential action on D2 presynaptic autoreceptors, male quail were pretreated with two different D2 antagonists (spiperone or pimozide; 0.5 or 2 mg/kg) before being injected with APO (100 micrograms or 1 mg/kg). Spiperone facilitated male sexual behavior but did not suppress the inhibitory effect of APO. No significant effect of pimozide was observed. These results support the notion that DA modulates male sexual activity in the Japanese quail. The specific role of the different dopaminergic receptor subtypes remains, however, to be elucidated. [less ▲]

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See detailAnaphylatoxin C5a Production During Short-Term Submaximal Dynamic Exercise in Man
Camus, G.; Duchateau, J.; Deby, Ginette ULg et al

in International Journal of Sports Medicine (1994), 15(1), 32-5

We studied the effects of short-term submaximal exercise on the plasma levels of myeloperoxidase ([MPO]) and C5a anaphylatoxin ([C5a]), taken as specific markers of polymorphonuclear neutrophil (PMN) and ... [more ▼]

We studied the effects of short-term submaximal exercise on the plasma levels of myeloperoxidase ([MPO]) and C5a anaphylatoxin ([C5a]), taken as specific markers of polymorphonuclear neutrophil (PMN) and complement activation, respectively. Eleven young, healthy male volunteers were subjected to a constant-load concentric exercise on a cycle ergometer (20 min at 80% maximal oxygen uptake). Mean resting MPO and C5a concentrations were 437 +/- 113 and 0.47 +/- 0.21 ng/ml, respectively. During exercise, [MPO] and [C5a] increased significantly (p < 0.001) towards respective peak values of 649 +/- 131 and 1.3 +/- 0.6 ng/ml. A rapid decrease of both [MPO] and [C5a] was observed during recovery. The similar time course of [MPO] and [C5a] changes and the highly significant relationship between these two variables (r = 0.651; p < 0.001) argues for the possible involvement of the complement anaphylatoxin C5a in the process of PMN degranulation. During exercise, the number of circulating PMN increased (+80%; p < 0.001) and remained practically unchanged up to 20 minutes of recovery. As [MPO] and PMN count were not significantly related (r = 0.2; p < 0.1), we concluded that the activation of PMN was independent of their mobilization. [less ▲]

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See detailDetection of Human Papillomaviruses in Paraffin-Embedded Biopsies of Cervical Intraepithelial Lesions: Analysis by Immunohistochemistry, in Situ Hybridization, and the Polymerase Chain Reaction
Delvenne, Philippe ULg; Fontaine, Marie-Anne; Delvenne, Catherine ULg et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (1994), 7(1), 113-119

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ ... [more ▼]

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (Vira-Type in situ assay; Digene Diagnostics, Silver Spring, MD) or probes labeled with digoxigenin by the random primer technique. Immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared to 61% (86/140) for the in situ assay with the digoxigenin-labeled probes. Biotinylated probes proved to be slightly less sensitive than digoxigenin-labeled probes, with a detection rate of 53% (74/140). Although less sensitive in our series taken as a whole, immunohistochemistry was positive in a few cases of CILs negative by in situ hybridization, so that the association of these techniques gave the highest detection rate (66%; 92/140). The CILs that remained negative with these methods (34%; 48/140) were investigated by the polymerase chain reaction (PCR) using consensus primers to determine definitively the presence of HPV in these lesions and were reviewed histologically to assess the diagnosis of CILs. The PCR method increased the detection rate of HPV in our series to 76% (107/140). The diagnosis of CILs was confirmed for all the biopsy specimens positive by PCR (15/15; 100%) and for all the HPV negative tissues with histological features of a high-grade lesions (7/7; 100%).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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