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See detailDevelopment of a Database System for the Management of Borehole Data : Experience and lessons
Halleux, Isabelle ULg

in Mathematical Geology (1994), 26(7), 833-841

CBS-LOG is a multilingual database system for the management of coal exploratory borehole data. It is a dBase application running on PC's with minimum configurations. Developing a suitably accurate ... [more ▼]

CBS-LOG is a multilingual database system for the management of coal exploratory borehole data. It is a dBase application running on PC's with minimum configurations. Developing a suitably accurate database requires some stratégic choices and lots of discussions and compromises between experts. The experience shows that it is absolutely necessary to follow strictly the development flow-sheet and that the difficulties rely mainly on communication and human aspects of mathematical geology. A step by step approach is reviewed in terms of experience and lessons. [less ▲]

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See detailHigh-Dose Riboflavin as a Prophylactic Treatment of Migraine: Results of an Open Pilot Study
Schoenen, Jean ULg; Lenaerts, M.; Bastings, E.

in Cephalalgia : An International Journal of Headache (1994), 14(5), 328-9

If the brain of migraineurs is characterized between attacks by a reduction of mitochondrial phosphorylation potential, riboflavin, which has the potential of increasing mitochondrial energy efficiency ... [more ▼]

If the brain of migraineurs is characterized between attacks by a reduction of mitochondrial phosphorylation potential, riboflavin, which has the potential of increasing mitochondrial energy efficiency, might have prophylactic effects in migraine. In this preliminary open pilot study, 49 patients suffering from migraine (45 without aura, 4 with aura) were treated with 400 mg of riboflavin as a single oral dose for at least 3 months. Twenty-three patients received in addition 75 mg of aspirin. Mean global improvement after therapy was 68.2% and there was no difference between the two groups of patients. With the exception of one patient in the riboflavin plus aspirin group who withdrew because of gastric intolerance, no drug-related side effects were reported. High-dose riboflavin could thus be an effective, low-cost prophylactic treatment of migraine devoid of short-term side effects. A placebo-controlled trial of its efficacy seems worthwhile. [less ▲]

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See detailPropofol Protects Cultured Rat Hippocampal Neurons against N-Methyl-D-Aspartate Receptor-Mediated Glutamate Toxicity
Hans, Pol ULg; Bonhomme, Vincent ULg; Collette, Julien ULg et al

in Journal of Neurosurgical Anesthesiology (1994), 6(4), 249-53

The effect of propofol on the toxicity induced by glutamate (GLU), N-methyl-D-aspartate (NMDA), kainate (KA), and amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) was investigated on cultured ... [more ▼]

The effect of propofol on the toxicity induced by glutamate (GLU), N-methyl-D-aspartate (NMDA), kainate (KA), and amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) was investigated on cultured fetal rat hippocampal neurons. The degree of neuronal injury was quantified by measuring the release of the neuron-specific enolase (NSE) into the culture media. The toxicity induced by brief exposure to GLU (10(-4) M) or to NMDA (10(-4) M) was significantly reduced by propofol, whereas that elicited by KA, AMPA (10(-4) M), or long GLU exposure was unaffected. In conclusion, high concentrations of propofol significantly attenuate NMDA receptor-mediated glutamate neurotoxicity in vitro. Further studies are needed to confirm this beneficial effect in vivo and to evaluate propofol as a neuroprotective anesthetic agent in pathologies involving glutamate release and NMDA-mediated toxicity. [less ▲]

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See detailAnalysis by in Situ Hybridization of Cytokine Mrna Expression in the Murine Developing Thymus
Deman, J.; Humblet, Chantal ULg; Martin, M. T. et al

in International Immunology (1994), 6(10), 1613-9

We have used in situ hybridization to investigate the expression of IL-1, IL-2, IL-4, IL-6 and IFN-gamma genes by thymic cells during fetal development in mice. Two waves of mRNAs were detected in thymic ... [more ▼]

We have used in situ hybridization to investigate the expression of IL-1, IL-2, IL-4, IL-6 and IFN-gamma genes by thymic cells during fetal development in mice. Two waves of mRNAs were detected in thymic cells for IL-1 at days 16 and 19 of gestation, for IL-2 at days 14 and 18, and for IL-4 at days 14 and 16. Three peaks for IL-6 were observed at days 13, 17 and around birth. Finally, only one peak of cells positive for IFN-gamma was detected. Whereas cells positive for IL-1 were generally grouped and more often localized in the external area of the thymus, the other positive cells were isolated and evenly distributed in the thymus. Our results illustrated the presence of cytokine transcripts in the developing thymus following a developmentally controlled sequence and support the hypothesis that cytokines could play a role in T cell development. [less ▲]

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See detailMolecular Structures of Penicillin-Binding Proteins and Beta-Lactamases
Ghuysen, Jean-Marie ULg

in Trends in Microbiology (1994), 2(10), 372-380

In the past, new antibacterial agents have been selected either from natural sources or by 'trial and error' modification of existing antibacterials. Future therapeutic strategies are likely to depend on ... [more ▼]

In the past, new antibacterial agents have been selected either from natural sources or by 'trial and error' modification of existing antibacterials. Future therapeutic strategies are likely to depend on increased knowledge of existing drug targets and the search for new targets. The machinery for the assembly of bacterial-cell-wall peptidoglycan is an ideal place to look. [less ▲]

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See detailDetermination of Benzodiazepines by Micellar Electrokinetic Chromatography
Bechet, I.; Fillet, Marianne ULg; Hubert, Philippe ULg et al

in Electrophoresis (1994), 15(10), 1316-21

A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and ... [more ▼]

A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and triethanolamine (pH 9.0), containing sodium dodecyl sulfate (SDS) as surfactant and methanol as organic modifier. The effect of the concentration of SDS, methanol, glycine and triethanolamine on migration times and resolution was studied. Ten benzodiazepines were baseline separated at a 25 mM SDS concentration and 20% v/v methanol in a 75 mM glycine-250 mM triethanolamine buffer. Under these conditions, the within-day reproducibilities were 0.3-0.5% for migration times and 1.7-1.9% for peak areas at a concentration of 10 micrograms/mL. The limits of detection and quantification for oxazepam were 0.2 and 0.7 micrograms/mL, respectively, using an injection time of 5 s. [less ▲]

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See detailFast determination of myoglobin in serum using a new radial partition immunoassay
Chapelle, Jean-Paul ULg; Lemache, K.; el Allaf, M. et al

in Clinical Biochemistry (1994), 27(5), 423-8

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See detailMesencephalic and third ventricle cysts: diagnosis and management in four cases.
RAMAEKERS, Vincent ULg; Reul, J.; Siller, V. et al

in Journal of neurology, neurosurgery, and psychiatry (1994), 57(10), 1216-20

Four infants with obstructive hydrocephalus caused by space occupying third ventricle and mesencephalic cysts are reported. Despite immediate shunt insertion in all patients, there was either lack of ... [more ▼]

Four infants with obstructive hydrocephalus caused by space occupying third ventricle and mesencephalic cysts are reported. Despite immediate shunt insertion in all patients, there was either lack of clinical improvement or late onset of clinical deterioration. Neuroimaging (CT, MRI, and ventriculography) diagnosed the presence of non-communicating midline outpouchings of the CSF pathways causing obstruction of aqueductal CSF flow and brainstem signs. The cysts were of different origin. In one patient it was caused by a previous thalamic haemorrhage, in another patient by neonatal Escherichia coli meningoventriculitis. In two cases with obstructive hydrocephalus at birth, the aetiology is unclear. Direct puncture and drainage of the cysts led to clinical improvement. The cysts were poorly visualised on CT and could be misinterpreted as an enlarged third ventricle, simulating congenital aqueduct stenosis. Careful neuroradiological investigation is necessary to establish an accurate diagnosis and neurosurgical management. In such cases with hydrocephalus and persisting ventricular enlargement despite shunting, CT ventriculography is a useful tool. [less ▲]

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See detailMolécules de la famille des protéases aspartiques dans le placenta des ruminants: hormones ou protéines ?
Beckers, Jean-François ULg; Roberts, R. Michael; Zoli, André Pagnah et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1994), 149(8-11), 355-367

The placenta of ruminant contains binucleate trophoblastic cells synthesizing proteins, migrating cross the barrier and fusing with endothelial cells of the endometrium. Recently described were two ... [more ▼]

The placenta of ruminant contains binucleate trophoblastic cells synthesizing proteins, migrating cross the barrier and fusing with endothelial cells of the endometrium. Recently described were two glycoproteins from the family of aspartic proteases, apparently lacking the enzymatic activity: the pregnancy associated glycorproteins I and II (PAGI and PAGII). The first (PAGI) is largely secreted in maternal blood, this characteristic copes with the lack of proteolytic activity. The second (PAGII) is not completely characterized. However, it binds to lutropin (LH) receptors with high affinity. This binding allows to assume that PAGII is likely the same as the bovine chorionic gonadotropin identified earlier (bCG). A better characterization of these glycoproteins (PAGI and PAGII) and other members of the family (PAGIII...) will answer these questions together with the unexplained invasive process of the placenta. [less ▲]

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See detailRole of Matrix, Fibroblasts and Type Iv Collagenases in Tumor Progression and Invasion
Noël, Agnès ULg; Emonard, H.; Polette, M. et al

in Pathology - Research & Practice (1994), 190(9-10), 934-41

We have studied the role of the extracellular matrix and host cells in tumor progression and tumor invasion. Our results emphasize the importance of tumoral cell-host cell interactions during this process ... [more ▼]

We have studied the role of the extracellular matrix and host cells in tumor progression and tumor invasion. Our results emphasize the importance of tumoral cell-host cell interactions during this process. Addition of human fibroblasts and/or basement membrane components to human mammary adenocarcinoma cells, when injected into athymic nude mice, results in an increase of take and growth rate of the tumors. Peritumoral extracellular matrix is remodeled through multiple mechanisms: overproduction of matrix components by fibroblasts, enhanced fibroblasts proliferation, modulation of interstitial collagenase production by fibroblasts and retraction of the matrix by tumoral cells. The degradation of basement membranes during the metastatic process is often associated with the secretion of proteolytic enzymes. The 72 kDa type IV collagenase, a metalloproteinase, can be produced by some tumoral cells. However, it appears also to be secreted by peritumoral stromal fibroblasts under the influence of tumoral cells. We have demonstrated the existence of a binding site for this enzyme on the membrane of mammary tumoral cells. These results suggest a cooperation between tumor cells and fibroblasts during basement membrane destruction. [less ▲]

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See detailSynthesis and characterization of biodegradable homopolymers and block copolymers based on 1,5-dioxepan-2-one
Löfgren, Anders; Albertsson, Ann-Christine; Dubois, Philippe et al

in Macromolecules (1994), 27

Homopolymers of 1,5-dioxepan-2-one (DXO) and block copolymers of DXO and epsilon-caprolactone (epsilon-CL) have been synthesized with aluminum isopropoxide as an initiator in toluene and tetrahydrofuran ... [more ▼]

Homopolymers of 1,5-dioxepan-2-one (DXO) and block copolymers of DXO and epsilon-caprolactone (epsilon-CL) have been synthesized with aluminum isopropoxide as an initiator in toluene and tetrahydrofuran (THF). The homopolymerization is first order with respect to both monomer and initiator, and the end-group analysis agrees with a coordination insertion mechanism based on the acyl-oxygen cleavage of the DXO ring. Living poly(epsilon-caprolactone) (PCL) and poly(1,5-dioxepan-2-one) (PDXO) chains are very efficient macroinitiators for the polymerization of DXO and epsilon-CL, respectively, with formation of block copolymers of a narrow molecular weight distribution. Size-exclusion chromatography (SEC) and C-13 NMR confirm the blocky structure of the copolymers, in agreement with DSC that show a melting endotherm for the PCL block and two glass transitions characteristic of the amorphous phases of PDXO and PCL. Because of the crystallinity of the PCL block (T(m) = 60-degrees-C) and the low glass transition temperature of the amorphous PDXO block (T(g) = -39-degrees-C), poly(epsilon-CL-b-DXO-b-epsilon-CL) triblocks have the potential of thermoplastic elastomers. Block copolymers of epsilon-CL and DXO are also sensitive to hydrolysis which makes them possible candidates for biomedical applications. Initiation of the DXO polymerization with functional diethylaluminum alkoxides is also discussed. [less ▲]

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See detailProfiles of stratospheric chlorine nitrate (ClONO2) from atmospheric trace molecule spectroscopy/ATLAS 1 infrared solar occultation spectra
Rinsland, Curtis P.; Gunson, M. R.; Abrams, M. C. et al

in Journal of Geophysical Research (1994), 99(D9), 18895-18900

Stratospheric volume mixing ratio profiles of chlorine nitrate (ClONO2) have been retrieved from 0.01-cm−1 resolution infrared solar occultation spectra recorded at latitudes between 14°N and 54°S by the ... [more ▼]

Stratospheric volume mixing ratio profiles of chlorine nitrate (ClONO2) have been retrieved from 0.01-cm−1 resolution infrared solar occultation spectra recorded at latitudes between 14°N and 54°S by the atmospheric trace molecule spectroscopy Fourier transform spectrometer during the ATLAS 1 shuttle mission (March 24 to April 2, 1992). The results were obtained from nonlinear least squares fittings of the ClONO2 v 4 band Q branch at 780.21 cm−1 with improved spectroscopic parameters generated on the basis of recent laboratory work. The individual profiles, which have an accuracy of about ±20%, are compared with previous observations and model calculations. [less ▲]

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See detailModulation of Temporalis Muscle Exteroceptive Suppression by Limb Stimuli in Normal Man
Schoenen, Jean ULg; Wang, W.; Gérard, P.

in Brain Research (1994), 657(1-2), 214-20

The effects of noxious and non-noxious limb stimulations on the second exteroceptive suppression of voluntary temporalis muscle activity (ES2) were studied in healthy human volunteers. Duration of ... [more ▼]

The effects of noxious and non-noxious limb stimulations on the second exteroceptive suppression of voluntary temporalis muscle activity (ES2) were studied in healthy human volunteers. Duration of temporalis ES2 was measured on averaged rectified responses obtained after stimulating the labial commissure at an intensity of 25 mA. Single peripheral electrical stimuli applied over nerve trunks or over the skin before the labial stimulus decreased ES2 duration. This effect was most pronounced after cutaneous stimuli, especially of the index finger, and it was not observed when the conditioning stimulus was a 10 second, high frequency train. For stimulation at the index finger, temporalis ES2 inhibition progressively increased with intensity from 10 mA to 40 mA; it was maximal for an interstimulus interval between 50 and 140 ms. After naloxone (0.4 mg or 4 mg, i.v.) there was a partial reversal of the index-induced ES2 depression, but this effect was not significant. Immersion of one hand in water heated at 47 degrees C produced a short-lasting ES2 reduction. These results are comparable, though not similar, to the inhibition of the digastric reflex (or jaw opening reflex) observed in animals after limb stimuli and to the depression of the spinal flexion reflex reported in man after heterotopic peripheral stimuli. Although peripheral stimuli were able by themselves to suppress temporalis EMG activity in some subjects, it is likely that they reduce labial-induced ES2 via activation of brainstem structures, such as periaqueductal gray matter or raphe magnus nucleus, which are thought to inhibit the medullary inhibitory interneurons mediating ES2. [less ▲]

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See detailEffects of Steroidal and Non Steroidal Aromatase Inhibitors on Sexual Behavior and Aromatase-Immunoreactive Cells and Fibers in the Quail Brain
Foidart, Agnès ULg; Harada, N.; Balthazart, Jacques ULg

in Brain Research (1994), 657(1-2), 105-23

Castrated quail were treated with Silastic implants filled with testosterone (T) in association with injections of the aromatase inhibitors, R76713 (racemic vorozole; 1 mg/kg twice a day) or 4 ... [more ▼]

Castrated quail were treated with Silastic implants filled with testosterone (T) in association with injections of the aromatase inhibitors, R76713 (racemic vorozole; 1 mg/kg twice a day) or 4-hydroxyandrostenedione (OHA; 5 mg/bird twice a day). [less ▲]

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See detailErratum for : primary structure of the streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene
Duez, Colette ULg; Piron-Fraipont, C.; Joris, Bernard ULg et al

in European Journal of Biochemistry (1994), 224(3), 1079

This is the correction of the fig. 5 of Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces Zividuns and nucleotide sequence of the gene, by C. Duez, C ... [more ▼]

This is the correction of the fig. 5 of Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces Zividuns and nucleotide sequence of the gene, by C. Duez, C. Piron-Fraipont, B. Joris, J. Dusart, M. S. Urdea, J. A. Martial, J.-M. Frère and J.-M. Ghuysen. European Journal of Biochemistry Volume 162, Issue 3, pages 509–518, February 1987 [less ▲]

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See detailDifferentiation Ability and Oncogenic Potential of Hpv-33- and Hpv-33 + Ras-Transfected Keratinocytes
Gilles, Christine ULg; Piette, Jacques ULg; Peter, W. et al

in International Journal of Cancer = Journal International du Cancer (1994), 58(6), 847-54

Five HPV-33-immortalized and 5 HPV-33 + ras-transfected cell lines were characterized in terms of growth in soft agar, tumorigenic potential in nude mice, p21 expression, morphology and expression of ... [more ▼]

Five HPV-33-immortalized and 5 HPV-33 + ras-transfected cell lines were characterized in terms of growth in soft agar, tumorigenic potential in nude mice, p21 expression, morphology and expression of differentiation markers in organotypic cultures. No striking differences were observed between the HPV-33-immortalized cell lines and their corresponding ras-transfected counterparts as regards their tumorigenicity in nude mice (only one cell line was able to develop tumors in nude mice) or their behavior on lifted collagen gels. However, all the ras-transfected cell lines gave rise to colonies in soft agar while only 2 HPV-33-transfected lines (CK1 and CK4) displayed this property. The 10 cell lines could be divided into 2 groups with respect to their phenotype in monolayer and in organotypic cultures. Lines from group I (CK2, 3, 5 and their ras-transfected homologous lines) shared a typical epithelial phenotype in monolayer and the ability (a) to form an epithelium similar to a CIN-III lesion and (b) to strongly express keratins K1-K10 and involucrin in organotypic cultures. On the other hand, for the lines from group II (CK1, CK4, CK1EJ7 and CK4EJ5), there was a correlation between an elongated phenotype in monolayer and the property (a) to form a structure similar to a microinvasive carcinoma and (b) to express vimentin and keratins K8-K18. These cell lines, exhibiting various transformation-associated alterations, can be considered as an in vitro model representing various stages of HPV-33-associated cervical carcinogenesis. [less ▲]

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See detailSynergistic Control by Androgens and Estrogens of Aromatase in the Quail Brain
Balthazart, Jacques ULg; Stoop, R.; Foidart, Agnès ULg et al

in Neuroreport (1994), 5(14), 1729-32

Castrated quail were injected with testosterone or with the synthetic hormones diethylstilbestrol (DES) or methyltrienolone (R1881) to analyse the steroid specificity in the induction of brain aromatase ... [more ▼]

Castrated quail were injected with testosterone or with the synthetic hormones diethylstilbestrol (DES) or methyltrienolone (R1881) to analyse the steroid specificity in the induction of brain aromatase. R1881 produced a moderate (generally non-significant) increase in the number of aromatase-immunoreactive cells. DES significantly increased the number of positive cells in most brain areas. A clear synergism between DES and R1881 was observed in all brain regions: more immunoreactive cells were found in birds receiving both compounds than in those injected with DES or R1881 alone. DES and R1881 are highly specific ligands for oestrogen and androgen receptors respectively. It appears likely that both androgens and oestrogens directly modulate brain aromatase, presumably at the transcription level. [less ▲]

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See detailMagnetic ordering in U2Pd2In and U2Pd2Sn
Purwanto, A.; Robinson, R. A.; Havela, Ladislav et al

in Physical Review. B : Condensed Matter (1994), 50(10), 6792-6801

The tetragonal intermetallic compounds U2Pd2In and U2Pd2Sn have been studied by means of magnetic susceptibility, magnetization, specific heat, electrical resistivity, and x-ray- and neutron-diffraction ... [more ▼]

The tetragonal intermetallic compounds U2Pd2In and U2Pd2Sn have been studied by means of magnetic susceptibility, magnetization, specific heat, electrical resistivity, and x-ray- and neutron-diffraction techniques. The compounds order antiferromagnetically at 36 and 41 K, respectively, and have enhanced electronic specific-heat coefficients γ of 393 and 203 mJ/mol f.u. K**2, respectively. At low temperature, the nearest-neighbor U-U spacing is along the c axis in both compounds, though in U2Pd2Sn the in-plane U-U distance is shorter at room temperature. This crossover is due to motion of the U atoms within the cell rather than anisotropy of the thermal contraction. It is now well established in several classes of (1:1:1) uranium ternary intermetallic compounds that the strong magnetic anisotropy is correlated with the direction of nearest-neighbor U-U links; the moments are almost invariably oriented perpendicular to such links. This is also the case for U2Pd2In and U2Pd2Sn, where the moments lie in the tetragonal basal plane in a canted arrangement along [110] and [1¯10] directions. Of four such Shubnikov subgroups of P4/mbm, one is clearly preferred by the data. At 10 K, the magnetic moments extracted are (1.6±0.2) Bohr magneton and (2.0±0.1) Bohr magneton per U atom in U2Pd2In and U2Pd2Sn, respectively. [less ▲]

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See detailComment je traite un patient hypertendu diabétique de type II
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(9), 473-474

1. Les patients diabétiques de type II se caractérisent par une prévalence élevée d'hypertension artérielle avec ses conséquences vasculaires sous-tendues par l'insulinorésistance. 2. Le traitement de l ... [more ▼]

1. Les patients diabétiques de type II se caractérisent par une prévalence élevée d'hypertension artérielle avec ses conséquences vasculaires sous-tendues par l'insulinorésistance. 2. Le traitement de l'hypertension artérielle constitue une mesure importante de prévention des complications cardiovasculaires, à côté de la correction d'une dyslipémie, de l'arrêt du tabagisme et de l'équilibre glycémique. 3. L'approche non pharmacologique constitue une première étape obligée mais efficace. 4. Le niveau de pression artérielle à atteindre est inférieur à 130/85 mmHg chez ce type de patient. 5. Le traitement médicamenteux est difficile, lié au risque accru d'effets métaboliques et vasculaires secondaires. 6. Les inhibiteurs de l'enzyme de conversion, les antagonistes calciques et les nouveaux bêtabloquants à propriété vasodilatatrice constituent un excellent premier choix. L'âge et d'éventuelles complications athéroscléreuses, déjà présentes au moment du traitement, orientent ce choix. [less ▲]

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See detailMesure ambulatoire de la pression artérielle, son utilité en pratique quotidienne
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(9), 475-480

Si la nécessité du traitement de l'hypertension artérielle est un fait accepté de façon universelle, la question à propos des niveaux de pression artérielle qui méritent une intervention reste débattue ... [more ▼]

Si la nécessité du traitement de l'hypertension artérielle est un fait accepté de façon universelle, la question à propos des niveaux de pression artérielle qui méritent une intervention reste débattue. Cette difficulté trouve son origine dans une telle variabilité de la pression artérielle que les mesures au cabinet de consultation représentent seulement une vue limitée de son réel profil sur 24 heures. Le développement d'enregistreurs portatifs de la pression artérielle par méthode non invasive a permis d'étudier la variabilité remarquable de cette pression artérielle tout au long des activités habituelles des patients. Bien que cette technique de mesure ambulatoire de la pression artérielle (MAPA) ne remplace pas la mesure traditionnelle de la pression artérielle, elle s'impose de plus en plus comme complément indispensable et non invasif dans certaines indications cliniques spécifiques (hypertensions de la blouse blanche, limite, réfractaire, ou encore vérification de l'efficacité du contrôle tensionnel). [less ▲]

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See detailLa prothèse pénienne: Contrariétés d'une virilité artificielle
Kempeneers, Philippe ULg; Andrianne, R.; Mormont, Christian ULg

in Sexologies (1994), 3(13), 26-30

Au cours d'une enquête de follow-up réalisée auprès de 25 patients implantés, nous avons constaté, en filigrane des réponses fournies par les sujets, un sentiment de malaise, voire d'humiliation lié à l ... [more ▼]

Au cours d'une enquête de follow-up réalisée auprès de 25 patients implantés, nous avons constaté, en filigrane des réponses fournies par les sujets, un sentiment de malaise, voire d'humiliation lié à l'usage d'une prothèse pénienne. Chez l'humain, l'érection comporte une valeur symbolique incontestable. Mise à mal par l'impuissance, l'image virile de l'homme ne se restaure pas entièrement avec l'installation d'une prothèse pénienne.Solution artificielle, celle-ci semble laisser subsister chez de nombreux patients des difficultés psychologiques à assumer la perte définitive de leurs capacités naturelles d'érection. [less ▲]

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See detailThe identification of 16O 18O 16O and 16O 16O 18O ozone isotopes in high resolution ground-based FTIR spectra
Arlander, Dixon William (Bill); Barbe, Alain; Bourgeois, M.T. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (1994), 52(3-4), 267271

We present evidence of the 16O 18O 16O and 16O 16O 18O ozone isotopes in the 5 μ region from FTIR solar occultation spectra obtained from the Jungfraujoch Solar Observatory (47°N, 8°E, 3580 m) in ... [more ▼]

We present evidence of the 16O 18O 16O and 16O 16O 18O ozone isotopes in the 5 μ region from FTIR solar occultation spectra obtained from the Jungfraujoch Solar Observatory (47°N, 8°E, 3580 m) in Switzerland at a spectral resolution of 0.0025 cm-1 (res.=1/2L). These spectra clearly show numerous unblended lines of the 16O 18O 16O ozone isotope. Laboratory spectra in the 5 μ region of both 50O3 isotopes have been measured and have yielded line positions of the v1+v3 isotopic bands which can eventually lead to their retrieval from measured ground-based solar occulation spectra. Although retrieval of 16O 16O 18O could be difficult due to slight line blending, identification of several lines is shown. [less ▲]

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See detailDramatic improvement of severe dilated cardiomyopathy in an acromegalic patient after treatment with octreotide and trans-sphenoidal surgery.
Legrand, Victor ULg; Beckers, Albert ULg; Pham, V. T. et al

in European Heart Journal (1994), 15(9), 1286-9

Severe congestive heart failure developed in an acromegalic patient, and was successfully treated with octreotide followed by trans-sphenoidal surgery. Clinical, hormonal echocardiographic and ... [more ▼]

Severe congestive heart failure developed in an acromegalic patient, and was successfully treated with octreotide followed by trans-sphenoidal surgery. Clinical, hormonal echocardiographic and haemodynamic findings as well as histological heart examination before and after treatment revealed that over-production of growth hormone may induce the myocardial cell degeneration responsible for mechanical heart dysfunction. In addition, this unique example demonstrates the reversibility of myocardial damage following octreotide and trans-sphenoidal surgery, leading to significant improvement in cardiac function with minimal diastolic dysfunction and moderate interstitial fibrosis. [less ▲]

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See detailCytokine Modulation of Basophil Histamine Release in Wasp-Venom Allergy
Radermecker, Maurice ULg; Louis, Renaud ULg; leclercq, M. et al

in Allergy (1994), 49(8), 641-4

We report the effect of interleukin-3 (IL-3) and of other cytokines on antigen-induced basophil histamine release in wasp-venom-allergic subjects. Leukocytes from 12 patients with documented anaphylactic ... [more ▼]

We report the effect of interleukin-3 (IL-3) and of other cytokines on antigen-induced basophil histamine release in wasp-venom-allergic subjects. Leukocytes from 12 patients with documented anaphylactic sensitivity to wasp venom were preincubated in the presence or absence of IL-3, granulocyte/macrophage-colony stimulating factor (GM-CSF), IL-5, IL-8, or stem cell factor (SCF). Washed cells were then exposed to venom and to other secretagogues, and histamine release in the supernatant was measured fluorometrically. Preincubation of leukocytes with IL-3, GM-CSF, or IL-5 (0.02-2 ng/ml), but not with IL-8 and SCF, caused a dose-dependent enhancement of antigen-induced basophilic histamine release in all subjects tested. Mean maximum increase was about 100% for IL-3, IL-5, and GM-CSF. The priming effect of IL-3 was rapid, persisted up to 12 h, and was not accompanied by a change in cellular histamine. IL-3 had a comparable enhancing effect when basophils were triggered with anti-IgE or N-formylmethionylphenylalanine (FMP). By contrast, IL-3 had no effect on substance-P-induced histamine release. The significant enhancement of basophil releasability to antigen in wasp-venom allergy by very low concentrations of IL-3, GM-CSF, and IL-5 suggests that cytokines in the basophil (mast-cell?) microenvironment could be critical factors in determining the variability of sting reactions in Hymenoptera-venom-allergic subjects. [less ▲]

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See detailCompte rendu de « Robert SACRÉ, Les Negro Spirituals et les Gospel Songs, Paris, P.U.F. (Que sais-je ? 2791), 1993 »
Pirenne, Christophe ULg

in Bulletin de la Société liégeoise de Musicologie (1994), 86

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See detailDecrease of T-Lymphocyte Proliferation in Exercise-Induced Asthma
Bury, Thierry ULg; Corhay, Jean-Louis ULg; Louis, Renaud ULg et al

in Allergy (1994), 49(8), 605-10

The present study was designed to examine the effect of physical exercise on T-lymphocyte proliferation in patients with exercise-induced asthma (EIA). Indeed, a decrease in different immune functions is ... [more ▼]

The present study was designed to examine the effect of physical exercise on T-lymphocyte proliferation in patients with exercise-induced asthma (EIA). Indeed, a decrease in different immune functions is described in normal man after exercise. Thirty subjects (10 normal and 20 asthmatic subjects with or without EIA) underwent a submaximal exercise test on an electrically driven treadmill. Before and after this test, ventilatory variables were measured, and venous blood was taken to study plasma histamine (RIA) and spontaneous and phytohemagglutinin (PHA)-pulsed T-lymphocyte proliferation (mononuclear cells isolated on Ficoll-Hypaque; tritiated thymidine incorporation). Ten minutes after the end of the exercise, there was a significant FEV1 decrease only in asthmatic subjects with EIA (mean: 24 +/- 5%). In the same group, the mean plasma histamine level was 0.31 ng/ml-1 (+/- 0.06) before the challenge. It rose to 0.62 ng/ml-1 (+/- 0.14) 10 min after the end of the exercise (P < 0.05), and returned to normal limits 20 min after the test. In this group, there was also a significant decrease (by about 35%) of spontaneous and PHA-pulsed T-lymphocyte proliferation 2 and 4 h after the exercise. By contrast, exercise challenge had no effect on either plasma histamine level or T-lymphocyte proliferation in the normal group. Our results show a rapid and transient increase in plasma histamine in EIA. This was followed 2 and 4 h later by a significant decrease of T-lymphocyte proliferation. A possible relationship between these two phenomena is discussed. [less ▲]

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See detailPreservation of Cortical Microcirculation after Kidney Ischemia-Reperfusion: Value of an Iron Chelator
Defraigne, Jean-Olivier ULg; Pincemail, Joël ULg; Detry, Olivier ULg et al

in Annals of Vascular Surgery (1994), 8(5), 457-67

Treatment of suprarenal aneurysms and renal artery reconstructions are both responsible for normothermic ischemia of the kidney (during clamping) followed by reperfusion (declamping). During reflow ... [more ▼]

Treatment of suprarenal aneurysms and renal artery reconstructions are both responsible for normothermic ischemia of the kidney (during clamping) followed by reperfusion (declamping). During reflow through an organ undergoing ischemia the production of free radicals can be associated with cell injury and a no-reflow phenomenon characterized by perfusion defects after a period of transient hyperemia. The objectives of this study were to demonstrate the existence of this phenomenon in the kidney undergoing ischemia followed by reperfusion and to test the potential protection afforded by an iron chelator (desferrioxamine) since free radical reactions are catalyzed by iron. Adult New Zealand white rabbits were divided into the following three groups: group A, 15 minutes of ischemia plus 10 minutes of reperfusion; group B, 60 minutes of ischemia plus 10 minutes of reperfusion; and group C, 60 minutes of ischemia plus 10 minutes of reperfusion combined with infusion of desferrioxamine (50 mg/kg). Cortical microcirculation in the kidney was measured by laser Doppler flowmeter before ischemia and 1, 5, and 10 minutes after reperfusion. Vitamin E content was determined in the cortex of the left kidney after 10 minutes of reperfusion and compared with that of the right (control) kidney. After 1 minute of reperfusion the cortical microcirculatory flow was significantly increased in all three groups (reactive hyperemia). In groups A and C blood flow returned to preclamping values after 10 minutes of reperfusion; however, blood flow in group B remained significantly reduced (29.2% +/- 10.5%) after 5 minutes of reperfusion with a further reduction to 48.5% +/- 5.7% after 10 minutes. These findings were correlated with the dosage of vitamin E since the vitamin E content was greatly reduced by 46.7% +/- 7.8% in group B but did not change significantly in groups A and C. This study shows that 60 minutes of normothermic ischemia is followed by a significant reduction in cortical microcirculatory flow (no-reflow phenomenon). Infusion of an iron chelator (desferrioxamine), however, which decreases the intensity of lipid peroxidation induced by the free radicals, preserves the microcirculatory flow. [less ▲]

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See detailMoabs Mas516 and 5b5, Two Fibroblast Markers, Recognize Human Follicular Dendritic Cells
Bosseloir, A.; Heinen, Ernst ULg; Defrance, T. et al

in Immunology Letters (1994), 42(1-2), 49-54

Follicular dendritic cells (FDC) are only located within follicles of secondary lymphoid tissues. The origin of this peculiar cell type is not clearly defined. To contribute to this study, we applied two ... [more ▼]

Follicular dendritic cells (FDC) are only located within follicles of secondary lymphoid tissues. The origin of this peculiar cell type is not clearly defined. To contribute to this study, we applied two monoclonal antibodies (MAS516 and 5B5) considered as specific for fibroblasts to tonsil cryosections and to isolated follicular dendritic cells. On the basis of an enzyme cocktail digestion of human tonsils and a fractionation procedure on albumin gradients, FDC can be prepared in the form of cell aggregates with associated lymphoid cells. MAS516 reacts with surface membrane molecules expressed by human fibroblasts, tissue macrophages and peripheral blood monocytes. With immunoperoxidase assays on tonsil cryosections connective tissue cells and macrophages are stained. Inside germinal centres, heavy labelling of the light zone was found. The MAS516 staining pattern is very similar to that of specific FDC markers DRC-1 or BU10. All isolated FDC reacted with MAS516 antibody. 5B5, considered as a typical fibroblast marker, reacts with human prolyl-4-hydroxylase which is an intracellular enzyme related to collagen biosynthesis. In cryosections, interfollicular and capsular areas showed 5B5 positive connective tissue fibroblasts. In germinal centres, some cells presenting features of FDC were 5B5 positive. After cell separation, 25%-50% of the isolated FDC were labelled with this antibody. This positivity of some FDC for 5B5 antibody may support the idea of their fibroblastic origin. The combination of observations realized in situ and after cell purification ensured an unequivocal recognition and identification of FDC.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailModulation of Immunological Histamine Release from Human Lung Fragments by Stem Cell Factor, Il-3, Il-5 and Gm-Csf: Comparison with Human Leukocytes
Louis, Renaud ULg; Dowlati, A.; Weber, T. et al

in International Archives of Allergy & Immunology (1994), 105(1), 18-25

Because of the importance of cytokines in the regulation of allergic inflammation, we investigated the effects of SCF, IL-3, IL-5 and GM-CSF on immunological histamine release from sensitized human lung ... [more ▼]

Because of the importance of cytokines in the regulation of allergic inflammation, we investigated the effects of SCF, IL-3, IL-5 and GM-CSF on immunological histamine release from sensitized human lung fragments as well as human leukocytes. SCF (0.2-20 ng/ml) caused a concentration-related enhancement of anti-IgE (1/100) induced histamine release from lung fragments reaching maximally 64% at 20 ng/ml. In contrast, enhancement produced by IL-5, IL-3 and GM-CSF (0.2-20 ng/ml) was quite marginal and reached at best around 20% at the higher concentration, IL-5 being slightly more effective than IL-3 and GM-CSF. Further, SCF potentiated histamine release whatever the level of immunological control whereas potentiation by IL-5 primarily occurred when the amount of histamine release induced by the immunological control ranged between 5 and 10%. SCF acted synergistically with IL-5, producing a greater enhancement of histamine release than the sum of each cytokine used alone. Both SCF and, to a lesser extent, IL-5 potentiated anti-IgE-mediated histamine release regardless of passive sensitization of lung fragments. Unlike what was observed with lung fragments, IL-3, GM-CSF and to a lesser extent IL-5, were potent enhancing agents of anti-IgE (1/2,000)-induced histamine release from leukocytes. Maximal enhancement produced by IL-3 (20 ng/ml), GM-CSF (2 ng/ml) and IL-5 (20 ng/ml) reached 92%, 78% and 61%, respectively. By contrast, SCF (0.2-20 ng/ml) was ineffective on human leukocytes.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailDirect Evidence of Free Radical Production after Ischaemia and Reperfusion and Protective Effect of Desferrioxamine: Esr and Vitamin E Studies
Defraigne, Jean-Olivier ULg; Detry, Olivier ULg; Pincemail, Joël ULg et al

in European Journal of Vascular Surgery (1994), 8(5), 537-43

After surgical renal revascularisation, warm renal ischaemia due to renal artery cross-clamping contributes to postoperative renal dysfunction. After reperfusion, free radicals are thought to be a ... [more ▼]

After surgical renal revascularisation, warm renal ischaemia due to renal artery cross-clamping contributes to postoperative renal dysfunction. After reperfusion, free radicals are thought to be a significant cause of injury. Nevertheless, indisputable proof of free radical production is scarce, partly because of their transient nature. In this study, electron paramagnetic resonance and vitamin E levels were used to demonstrate the free radical production after renal ischaemia and reperfusion. Rabbit kidneys were submitted either to 15 or 60 minutes of ischaemia followed by reperfusion. A spin trap agent (alpha-phenyl-N-tert-butyl nitrone (PBN), 20mg/ml, 1 ml/min) was infused during reperfusion directly into the left renal artery via an aortic catheter before declamping. Blood samples were selectively drawn from the left renal vein for ESR analysis (Varian spectrometer E109) of lipidic residues extracted from blood samples. The vitamin E content of the left renal cortex was determined by HPLC procedure. The right renal cortex was used as a control for the vitamin E values. In the venous effluent, ESR analysis revealed the formation of a spectrum consisting of a triplet of asymmetric doublets. This signal resulted from the spin trapping by PBN of a mixture of both oxygen- and carbon- centred lipidic radicals. The amplitude of the signal which is proportional to the amount of free radicals was significantly higher after 60 minutes ischaemia than after 15 minutes.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailQuantitative Assessment of the Specific Cd4+ T Lymphocyte Proliferative Response in Bovine Herpesvirus 1 Immune Cattle
Denis, M.; Kaashoek, M. J.; van Oirschot, J. T. et al

in Veterinary Immunology and Immunopathology (1994), 42(3-4), 275-86

We quantified the CD4+ T cell proliferation specific for bovine herpesvirus 1 (BHV-1) in peripheral blood mononuclear cells from cattle. The stimulation index as detected in proliferative assays performed ... [more ▼]

We quantified the CD4+ T cell proliferation specific for bovine herpesvirus 1 (BHV-1) in peripheral blood mononuclear cells from cattle. The stimulation index as detected in proliferative assays performed in the presence of BHV-1 antigen is highly variable in immune cattle. By using proliferative assays performed after negative selection we showed that, as expected, CD4+ T cells were the limiting cell type for antigen-induced proliferation. Neither B, gamma delta T nor CD8+ cells seemed to be involved. The limiting dilution method was established to obtain quantitative estimations, namely frequencies of specific T cells. When limiting dilution cultures were supplemented with interleukin-2 (IL-2), an IL-2 induced unspecific cell proliferation masked the specific T cell proliferation. Natural killer cells were not the major cell type involved, but CD4+ lymphocytes themselves seemed to respond to IL-2 irrespective of the presence of antigen. When cultures were performed without addition of IL-2, the frequency of BHV-1 specific proliferative T cells could be obtained by the difference between the frequency of proliferating cells calculated in the presence and absence of antigen. The method provides a sensitive and quantitative means to measure the T cell immune response to BHV-1 vaccine candidates. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 17. Ligated anionic living polymerization of 2-ethylhexyl acrylate as promoted by polydentate lithium alkoxides
Wang, Jin-Shan; Bayard, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1994), 27(18), 4890-4895

Different initiators combined with a new family of mu/sigma dual ligands, i.e., polydentate alkoxides, were used to initiate the anionic polymerization of 2-ethylhexyl acrylate (2EtHA) at low temperatures ... [more ▼]

Different initiators combined with a new family of mu/sigma dual ligands, i.e., polydentate alkoxides, were used to initiate the anionic polymerization of 2-ethylhexyl acrylate (2EtHA) at low temperatures. It has been found that lithium 2(2-methoxyethoxy) ethoxide (LiOEEM) is the most efficient mu/sigma ligand in promoting a living polymerization of that monomer. Although LiOEEM gives rise to a molecularly well-controlled P2EtHA at -78 degrees C in a 9/1 toluene/THF mixture, i.e., quantitative yield, high initiator efficiency (>90%), and narrow molecular weight distribution (M(w)/M(n) down to 1.05), two-step monomer resumption experiments always lead to a bimodal distribution, suggesting that the LiOEEM-complexed P2EtHA anions are not stable. Nevertheless, a perfectly living 2EtHA anionic polymerization process has been obtained at -100 degrees C, keeping other conditions unchanged. In comparison with other types of ligands, such as LiCl and LiOtBu (both mu-ligands) and DB18CE6 and K222 (both sigma-ligands), it has been further demonstrated that the formation of a stable and bulky active complex is at the origin of the anionic living polymerization of 2EtHA, in agreement with our findings in the case of anionic statistical copolymerization of methacrylate and acrylate. [less ▲]

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See detailThe Precursor of the Streptomyces R61 Dd-Peptidase Containing a C-Terminal Extension Is Inactive
Fanuel, Laurence; Granier, Benoît; Wilkin, Jean-Marc et al

in FEBS Letters (1994), 351(1), 49-52

The Streptomyces R61 DD-peptidase gene encodes a 26-residue C-terminal extension which is not found in the mature protein. When the gene was expressed in Escherichia coli, the extension was not cleaved ... [more ▼]

The Streptomyces R61 DD-peptidase gene encodes a 26-residue C-terminal extension which is not found in the mature protein. When the gene was expressed in Escherichia coli, the extension was not cleaved and the precursor protein was not enzymatically active. It also reacted with penicillins significantly more slowly than the mature protein. The introduction of a 'stop' codon after that corresponding to the C-terminal residue of the mature protein resulted in the production of an active protein in the periplasm of E. coli. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 19. Effect of various types of ligands other than lithium chloride on the stereochemisty of anionic polymerization of methyl methacrylate
Wang, Jin-Shan; Jérôme, Robert ULg; Teyssié, Philippe

in Macromolecules (1994), 27(18), 4902-4907

The effect of several types of ligands, i.e., 12-CE4 (1), K211 (2), LiOtBu (3), and LiOEEM (4), on the stereochemistry of MMA anionic polymerization at -78 degrees C in various solvents, i.e., toluene ... [more ▼]

The effect of several types of ligands, i.e., 12-CE4 (1), K211 (2), LiOtBu (3), and LiOEEM (4), on the stereochemistry of MMA anionic polymerization at -78 degrees C in various solvents, i.e., toluene, THF, and a 9/1 toluene/THF mixture, has been studied. Similar to the LiCl-complexed system, the stereoregularity of MMA polymerization in the presence of these additives is seemingly controlled by association/complexation equilibria among associated, nonassociated, and ligand-complexed active species. Accordingly, complexation of active species by sigma-chelating ligands such as 12-CE4 and K211 simply shifts the association equilibrium toward the formation of ligand-complexed (nonassociated) species, giving preferably syndiotactic placements (rr) in a 9/1 toluene/THF mixture as well as in toluene. However, the effect of a mu-type ligand, i.e., LiOtBu, seems more complicated. In THF and toluene, similar to ligand-free systems, highly syndiotactic and isotactic PMMAs are produced, respectively, implying the existence of different types of stereoselective LiOtBu-complexed species. In a 9/1 toluene/THF mixture, moreover, addition of LiOtBu gives rise to an increase in isotactic placements (mm) and matches the values observed in toluene. This is in striking contrast to the LiCl-added system, for which it has been tentatively proposed that a meso or raceme placement might also critically depend on the aggregation degree of the living chain in the complexed species. Nevertheless, a mu/sigma dual ligand, i.e., LiOEEM, gives rise to an almost identical stereoregularity (i.e., highly syndiotactic) whatever the solvent used, well consistent with the involvement of the same type of active complex. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 18. NMR characterization of a unique complex between lithium 2-(2-methoxyethoxy) ethoxide and methyl α-lithioisobutyrate
Wang, Jin-Shan; Jérôme, Robert ULg; Teyssié, Philippe

in Macromolecules (1994), 27(18), 4896-4901

A unique ligand-promoted-loose mixed complex MIB-(Li-3(OEEM)(2))(+) has been characterized for the first time by means of NMR spectroscopy in solution. This new complex, which is a dual mu/sigma one, is ... [more ▼]

A unique ligand-promoted-loose mixed complex MIB-(Li-3(OEEM)(2))(+) has been characterized for the first time by means of NMR spectroscopy in solution. This new complex, which is a dual mu/sigma one, is obviously different from either a simple tight mu-mixed complex, such as nMIBLi.mX (X = Cl or OtBu), or a sigma-cation-binding one with mole charge separation, such as K211/MIBLi. It originates from a simultaneous dual complexation (mu/sigma) of MIBLi by both the lithium alkoxide (mu-mixed complexation) and the chelating polyether components (sigma-cation-binding complexation) of LiOEEM. Furthermore, it has been demonstrated; that, whichever the solvent, i.e., toluene, a 9/1 toluene/THF mixture, or THF, complexation of MIBLi by greater than or equal to 2 mol equiv of LiOEEM produces the same type of complex, i.e., MIB-(Li-3(OEEM)(2))(+), suggesting that, in this strong complex, the lithium cations are strongly coordinated by the ligand. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 21. Anionic sequential polymerization of 2-ethylhexyl acrylate and methyl methacrylate
Wang, Jin-Shan; Jérôme, Robert ULg; Bayard, Philippe et al

in Macromolecules (1994), 27(18), 4908-4913

A mixed complex of a polydentate lithium alkoxide, i.e., lithium 2-(2-methoxyethoxy) ethoxide (LiOEEM), and of a mono- or bisfunctional organolithium initiator, i.e., (diphenylmethyl)lithium (DPMLi) or ... [more ▼]

A mixed complex of a polydentate lithium alkoxide, i.e., lithium 2-(2-methoxyethoxy) ethoxide (LiOEEM), and of a mono- or bisfunctional organolithium initiator, i.e., (diphenylmethyl)lithium (DPMLi) or lithium naphthalene/diphenylethylene, has been used to synthesize well-controlled AB (BA), ABA, and BAB block copolymers of methylmethacrylate (MMA) (A) and 2-ethylhexyl acrylate (2EtHA) (B), irrespective of the monomer addition order. Although the 2EtHA block copolymerization initiated with monofunctional living PMMA macroanions at -78 degrees C in a 75/25 toluene/THF mixture gives rise to a precisely-tailored PMMA-b-P2EtHA diblock polymer, the reverse sequence, i.e.,from P2EtHA anions to the MMA type monomer, always results in contamination by homo-P2EtHA. This has been interpreted in terms of the short shelf lifetime of P2EtHA anions present. Nevertheless, a pure P2EtHA-b-PMMA type diblock copolymer can be produced at -100 degrees C, while keeping other conditions unchanged. More importantly, a well-controlled PMMA-b-P2EtHA-b-PMMA triblock copolymer has been prepared through a three-stage process with a monofunctional initiator, actually providing a potential pathway toward the direct synthesis of a novel type of fully acrylic thermoplastic elastomer, instead of the more complicated hydrolysis/transalcoholysis process previously demonstrated by us. [less ▲]

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See detailHuman xeroderman group G gene encodes a DNA endonuclease
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Nucleic Acids Research (1994), 22(16), 3312-6

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See detailSECULAR TREND AND SEASONAL VARIABILITY OF THE COLUMN ABUNDANCE OF N2O ABOVE THE JUNGFRAUJOCH STATION DETERMINED FROM IR SOLAR SPECTRA
Zander, Rodolphe ULg; EHHALT, D. H.; RINSLAND, Curtis P. et al

in Journal of Geophysical Research (1994), 99(D8), 16745-16756

Infrared solar spectra recorded at the International Scientific Station of the Jungfraujoch (3580 m altitude), Switzerland, in 1950-1951 and from 1984 to 1992 have been analyzed to determine vertical ... [more ▼]

Infrared solar spectra recorded at the International Scientific Station of the Jungfraujoch (3580 m altitude), Switzerland, in 1950-1951 and from 1984 to 1992 have been analyzed to determine vertical column abundances of nitrous oxide (N2O) above the station. The best fit to the relatively dense set of measurements made between 1984 and 1992 indicates a mean exponential rate of increase equal to 0.36 +/- 0.06% yr-1 (1 sigma) and a seasonal modulation of 7.2% peak to peak, the minimum occurring at the end of the winter and the maximum in early September. The column abundances for April of the years 1951, 1984, and 1992 were found equal to 3.49 x 10(18), 3.76 x 10(18), and 3.87 x 10(18) molecules cm-2, respectively; they translate into N2O concentrations at the altitude of the Jungfraujoch equal to 275, 296, and 305 parts per billion by volume. These results indicate that the exponential rate of increase for 1951-1984 was equal to 0.23 +/- 0.04% yr-1 (1 sigma), thus substantially lower than for the 1984-1992 time interval and that the so-called preindustrial levels of N2O pertained until 1951 with most of the increase in atmospheric N2O occurring thereafter. [less ▲]

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See detailIncrease of carbonyl fluoride (COF2) in the stratosphere and its contribution to the 1992 budget of inorganic fluorine in the upper stratosphere
Zander, Rodolphe ULg; Rinsland, Curtis P.; Mahieu, Emmanuel ULg et al

in Journal of Geophysical Research (1994), 99(D8), 16737-16743

Volume mixing ratio profiles of COF2 have been derived through most of the stratosphere between 30°N and 54°S from series of 0.01-cm−1 resolution infrared solar spectra recorded in the occultation mode by ... [more ▼]

Volume mixing ratio profiles of COF2 have been derived through most of the stratosphere between 30°N and 54°S from series of 0.01-cm−1 resolution infrared solar spectra recorded in the occultation mode by the atmospheric trace molecule spectroscopy (ATMOS) instrument during the ATLAS 1 space shuttle mission of March–April 1992. When compared with similar results obtained from the ATMOS/Spacelab 3 mission of April–May 1985, the cumulative increase in the burden of COF2 in the middle and upper stratosphere was found to be 67% for that 7-year time interval. By combining a subset of these COF2 results with upper stratospheric concentrations of HF also derived from the ATMOS observations, it was further found that the budget of inorganic fluorine above 35 km altitude increased by (60 ± 10) % over the 1985–1992 time interval, which corresponds to an average exponential rate of increase of (6.7 ± 1.1) % yr−1, or a linear rate of increase referenced to 1985 of (8.5 ± 1.3) % yr−1 at the 1σ confidence level. The total inorganic F atom volume mixing ratio found in the upper stratosphere for 1985 and 1992 and the increase during this period mirror the rise in man-made fluorine-bearing compounds at the ground during the early to mid 1980s. This demonstrates the negligible impact of natural sources of fluorine, in particular volcanic activity, on the observed change of F in the upper stratosphere. Implications of the present findings and comparison with model results are discussed. [less ▲]

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See detailSite-directed mutagenesis of penicillin-binding protein 3 of Escherichia coli: role of Val-545
Ayala, Juan; Goffin, Colette; Nguyen-Distèche, Martine ULg et al

in FEMS Microbiology Letters (1994), 121(2), 251-256

Val545 of the Escherichia coli penicillin-binding protein 3 is essential to the acyl transfer mechanism through which the active-site serine 307 is acylated by benzylpenicillin and cephalexin and to the ... [more ▼]

Val545 of the Escherichia coli penicillin-binding protein 3 is essential to the acyl transfer mechanism through which the active-site serine 307 is acylated by benzylpenicillin and cephalexin and to the mechanism through which the protein allows rapidly growing cells to divide. [less ▲]

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See detailTraitement médicamenteux chronique de l'asthme
Louis, Renaud ULg; Bury, Thierry ULg; Radermecker, Maurice ULg

in Revue Médicale de Liège (1994), 49(8), 429-30

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See detailAuroral Lyman alpha and H2 bands from the giant planets: 1. Excitation by proton precipitation in the Jovian atmosphere
Rego, Daniel; Prange, Renee; Gérard, Jean-Claude ULg

in Journal of Geophysical Research (1994), 99

This paper is part of a work aimed at modeling the ratio of the observed Jovian auroral intensity at H Lyman-alpha and in the H2 Lyman and Werner bands and interpreting them as diagnostic of the incident ... [more ▼]

This paper is part of a work aimed at modeling the ratio of the observed Jovian auroral intensity at H Lyman-alpha and in the H2 Lyman and Werner bands and interpreting them as diagnostic of the incident magnetospheric particle species and energy. The work is planned in three steps: (1) modeling of the volume excitation rate, (2) modeling of the radiative transfer at Lyman-alpha, (3) application to existing observational data and new data obtained from the Hubble Space Telescope. The present paper deals with the first step. Models of the volume excitation rate have previously been developed for low energy electrons and oxygen ions. However, the energy range of the study has to be extended towards higher energy in view of recent results on the penetration depth of the primary particles. Protons have not been modeled so far. We have used an existing electron code of degradation of energy (Gerard and Singh, 1982) which has been improved, updated and adapted to the case of precipitating protons. The issues of nonequilibrium beam H/H(+) fractions and of getting reliable cross sections over a wide energy range have been considered with particular care. The altitude distribution of the volume excitation rate is compared for electrons and protons, for various initial energies in the range 10-50 keV and 50 keV to 1 MeV, respectively. [less ▲]

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See detailComment je traite un hypertendu avec néphropathie diabétique
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(8), 427-428

1. La néphropathie diabétique, complication tardive du diabète, se caractérise par une protéinurie supérieure à 300 mg/24 heures. Elle n'affecte cependant pas tous les diabétiques. 2. Lorsqu'elle apparaît ... [more ▼]

1. La néphropathie diabétique, complication tardive du diabète, se caractérise par une protéinurie supérieure à 300 mg/24 heures. Elle n'affecte cependant pas tous les diabétiques. 2. Lorsqu'elle apparaît, l'hypertension est fréquente, cette dernière aggravant l'involutivité de l'atteinte rénale. 3. Un contrôle tensionnel optimal ralentit son évolution. 4. Les inhibiteurs de l'enzyme de conversion utilisés tôt, notamment au stade de la microalbuminurie sans hypertension artérielle, possèdent, à côté de leur action antihypertensive, des propriétés antiprolifératives et antiprotéinuriques freinant la dégradation fonctionnelle rénale. 5. Les antagonistes calciques, surtout autres que les dérivés dihydropyridine, peuvent constituer une alternative en cas de contreindication à l'utilisation des inhibiteurs de l'enzyme de conversion. 6. A un stade avancé, le contrôle tensionnel, quelle qu'en soit la manière, joue le rôle protecteur principal sur les reins. [less ▲]

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See detailThe 6-Kilobase C-Erbb2 Promoter Contains Positive and Negative Regulatory Elements Functional in Human Mammary Cell Lines
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Dijkmans, Huguette et al

in Cancer Research (1994), 54(15), 4193-9

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3 ... [more ▼]

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3'-ends were obtained by progressive unidirectional deletion from the 5'-end and were cloned in front of the luciferase reporter gene. The hybrid genes were analyzed for transcriptional activity in human mammary cell lines synthesizing low (HBL-100 and T-47D), moderate (MDA-MB-453), or high (BT-474) amounts of the c-erbB2 mRNA and were also analyzed in HeLa cells. Gene-specific expression was observed, indicating the presence of multiple cis-acting sequences in the c-erbB2 promoter. A major negative element is located in the -2- to -4-kilobase region. It is flanked on both sides by positive elements that display enhanced transcriptional activity in the BT-474 tumor cells only. While predominant in the low-expressing cells, the effect of the repressor appears to be overcome by the distal transactivator in the high-expressing BT-474 cells, resulting in a 15 to 50 times increase in luciferase activity relative to the HBL-100 and T-47D cells, respectively. Cell-specific expression relies on the trans-acting factors present in the different cell lines. The formation of cell-specific protein-DNA complexes was demonstrated by gel retardation assay. [less ▲]

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See detailAnionic polymerization of acrylic monomers. 15. Living anionic copolymerization of mixtures of methyl metacrylate and tert-butyl acrylate as promoted by dibenzo-18-crown-6
Wang, Jin-Shan; Jérôme, Robert ULg; Bayard, Philippe et al

in Macromolecules (1994), 27(16), 4615-4620

Although mu-coordinating ligands such as LiCl are effective in promoting the well-controlled living anionic block copolymerization of methyl methacrylate (MMA) and tert-butyl acrylate (tBuA), whichever ... [more ▼]

Although mu-coordinating ligands such as LiCl are effective in promoting the well-controlled living anionic block copolymerization of methyl methacrylate (MMA) and tert-butyl acrylate (tBuA), whichever monomer is first polymerized, it has been previously shown that a copolymerization of mixtures of these two monomers does not proceed as expected, giving poor results in terms of both conversion and molecular weight distribution: that was accounted for by the easy back-biting nucleophilic attack of a highly sensitive methyl ester group by a moderately hindered PtBuA anion. It is now reported that such a situation can be completely modified, when a cation-binding sigma-ligand, i.e., dibenzo-18-CE-6, is used in chelating a sodium counterion and surrounding it with a steric barrier, blocking a large enough space area around the ion pair. In fact, a living copolymerization process of mixtures of MMA and tBuA prevails in THF at -78-degrees-C, in the presence of that Na+/DB-18-CE-6 system. The living copolymer is found, although in a relatively small extent, to be of a statistical type rather than a blocky one or a mixture of two corresponding homopolymers. Furthermore, using the extended Kelen-Tudos method, the monomer reactivity ratios are determined to be r(MMA) = 0.02 and r(tBuA) = 8.81. [less ▲]

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See detailA 5-Year Controlled Randomized Study of Prevention of Postmenopausal Trabecular Bone Loss with Nasal Salmon Calcitonin and Calcium
Reginster, Jean-Yves ULg; Meurmans, L.; Deroisy, Rita ULg et al

in European Journal of Clinical Investigation (1994), 24(8), 565-9

The aim of this paper was to evaluate the long-term (5 years) efficacy of nasal salmon calcitonin in prevention of trabecular postmenopausal bone loss, which was a follow-up of a previously published ... [more ▼]

The aim of this paper was to evaluate the long-term (5 years) efficacy of nasal salmon calcitonin in prevention of trabecular postmenopausal bone loss, which was a follow-up of a previously published study (3 years); a randomized, controlled group comparison. One hundred healthy postmenopausal women were randomly chosen from those (186) having completed the 3 year protocol. The 100 women were allocated to an additional 2 year period (total of 5 years) of treatment with either 500 mg d-1, 5 days week-1 of calcium or the same amount of calcium plus 50 IU d-1, 5 days per week of nasal salmon calcitonin, 87 (87%) women complied with the protocol throughout. The main outcome measures were the bone mineral density of the lumbar spine (1-BMD) (DPA) and biochemical parameters reflecting bone turnover (serum alkaline phosphatases, urinary calcium/creatinine and hydroxyproline/creatinine ratios). The women receiving calcium alone presented a significant decrease in 1-BMD after 6 months [-1.6 (0.5)%] [mean(SEM)] (P < 0.01) and this decrease remained significant after 36 months [-6.1(0.8)%] (P < 0.01) and until the end of the trial [-6.6(1.0)% at t60] (P < 0.01). In women receiving calcium and calcitonin, 1-BMD significantly increased after 36 months [+2(0.7%] (P < 0.01) and 42 months [+2.5(0.7)%] (P < 0.01 and was unchanged at the other times of investigation [+1.1 (1.1)% at t60] (NS). The evolution of BMD in the two groups was highly significantly different (P < 0.001) since the sixth month of the study and remained so until the end of the study.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailImprovement of Gc-Rich Template Amplification by Inverse Pcr
Moreau, Alain; Duez, Colette ULg; Dusart, Jean

in BioTechniques (1994), 17(2), 232-4

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See detailEffect of topographic stress on circulation in the Western Mediterranean
Alvarez, A.; Tintore, J.; Holloway, G. et al

in Journal of Geophysical Research (1994), 99(C8), 16053-16064

The interaction of eddies with seafloor topography can exert large systematic forces on ocean circulation. Using a statistical mechanics approach it is possible to obtain a parametrization of this effect ... [more ▼]

The interaction of eddies with seafloor topography can exert large systematic forces on ocean circulation. Using a statistical mechanics approach it is possible to obtain a parametrization of this effect (the Neptune effect) for application in large-scale ocean circulation models. Circulation of the western Mediterranean has been observed to follow a definite cyclonic path. Numerical models usually show good qualitative agreement for the large-scale circulation but show systematic deficiencies at a subbasin scale. We have tested the importance of the Neptune effect on the circulation of the western Mediterranean Sea. To perform this test, different numerical experiments on western Mediterranean circulation were done with and without eddy-topography interaction. As a first step we analyze the influence of the Neptune effect in an ideal western Mediterranean with closed straits. After these experiments the more realistic case of open straits is studied. All the experiments show that the Neptune effect may be a significant factor in the basin and subbasin scale circulation in the western Mediterranean Sea. [less ▲]

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See detailIdentification of Clostridium tyrobutyricum and related species using sugar fermentation, organic acid formation and DNA probes based on 16S rRNA sequences
Klijn, N.; Bovie, C.; Dommes, Jacques ULg et al

in Systematic and Applied Microbiology (1994), 17(2), 249-256

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See detailSelenium deficiency triggering intractable seizures
RAMAEKERS, Vincent ULg; Calomme, M.; Vanden Berghe, D. et al

in Neuropediatrics (1994), 25(4), 217-23

Two children with severe neurodevelopmental retardation and elevated liver function tests developed intractable seizures during the first year of life. Detectable neurometabolic conditions have been ruled ... [more ▼]

Two children with severe neurodevelopmental retardation and elevated liver function tests developed intractable seizures during the first year of life. Detectable neurometabolic conditions have been ruled out. At the time of seizures evidence for systemic selenium deficiency could be documented. The youngest patient, who manifested intractable fits from the fourth day of life, died at the age of ten months. Neuropathologic examination was consistent with Progressive Neuronal Degeneration of Childhood (PNDC) with liver disease or formerly known as Alpers disease. In the oldest child, whose diet was normally balanced, fits started from the age of 11 months and features of long-standing selenium deficiency became apparent from the age of 1 1/2 years and consisted of liver function disturbances, depigmented hair and osteoarthropathy. Oral substitution with selenium supplements in both children (3-5 micrograms/kg body weight) resulted in reduction of seizures and improvement of the EEG recordings after two weeks while liver function became normal. Two of the seleno-dependent enzymes Glutathione Peroxidase (GPX) and Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPX) are speculated to play a key-role in the defence of neuronal cells against oxygen radical formation and peroxidative processes. Our findings support the hypothesis that the presence of selenium depletion in the brain amongst patients with epilepsy constitutes an important triggering factor for the origin of intractable seizures and subsequent neuronal damage. [less ▲]

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See detailLacunarity, fractal, and magnetic transition behaviors in a generalized Eden growth-process
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1994), 50(2), 635-638

The effect of an internal extra degree of freedom introduced into the simplest growth model (the Eden model) leads to a joint physical and geometrical transition. Lacunarity, fractal, and magnetic ... [more ▼]

The effect of an internal extra degree of freedom introduced into the simplest growth model (the Eden model) leads to a joint physical and geometrical transition. Lacunarity, fractal, and magnetic transitions are indeed reported to be at the same critical values of the growth parameters. A logarithmic behavior of the cluster mass on this critical value was found. [less ▲]

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See detailLa déficience en hormone de croissance chez l'adulte et son traitement
Moreau, L.; Beckers, Albert ULg

in Médecine et Hygiène (1994), 52(2036), 1660-4

Les adultes déficients en hormone de croissance (GH) décèdent prématurément, principalement à cause de la fréquence élevée des affections cardiovasculaires. Depuis que la GH est produite par les ... [more ▼]

Les adultes déficients en hormone de croissance (GH) décèdent prématurément, principalement à cause de la fréquence élevée des affections cardiovasculaires. Depuis que la GH est produite par les techniques de biologie moléculaire, il est possible de proposer un traitement substitutif. Ce traitement améliore le bilan lipidique, entraîne une redistribution de la graisse d'androïde en gynoïde, une réduction de la masse graisseuse, une augmentation de la masse des tissus maigres et du volume de l'eau extracellulaire. Le poids des patients est légèrement réduit par le traitement substitutif. La masse de muscle strié squelettique est augmentée, ainsi que le contenu osseux (BMC) et la densité osseuse (BMD). La fonction cardiaque et la qualité de vie sont améliorées. [less ▲]

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See detailAcute Polymyopathy after Carbamate Poisoning in a Dog
Mc Entee, Kathleen ULg; Poncelet, Luc; Clercx, Cécile ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1994), 135(4), 88-90

The acute polymyopathy in a seven-year-old German shepherd dog was attributed to the muscular hypertonia, tremors and seizures which developed during the acute phase of carbamate poisoning. After two days ... [more ▼]

The acute polymyopathy in a seven-year-old German shepherd dog was attributed to the muscular hypertonia, tremors and seizures which developed during the acute phase of carbamate poisoning. After two days of generalised muscular rigidity, the dog adopted a characteristic fetal position which could be explained by the imbalance between the injuries to the extensor and flexor muscles. The polymyopathy resolved gradually over the course of a week. [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 18. Synthesis of star-branched aliphatic polyesters bearing various functinal end groups
Tian, Dong; Dubois, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1994), 27(15), 4134-4144

Three-arm star-branched aliphatic polyesters have been synthesized by an appropriate termination reaction of the living chains prepared by ring-opening polymerization with aluminum alkoxides. The use of ... [more ▼]

Three-arm star-branched aliphatic polyesters have been synthesized by an appropriate termination reaction of the living chains prepared by ring-opening polymerization with aluminum alkoxides. The use of functional aluminum alkoxides, Et2AlOCH2X with X = CH2CH2CH=CH2 and (CH2)10Br, has led to X functional end-capped star-branched polyesters. Trimesic acid trichloride has proved to be an efficient termination agent for the living poly(epsilon-caprolactone) [PCL] chains ended with a reactive aluminum alkoxide group. A quantitative end functionalization has been reported for bromide and 1-pentene end-capped star-branched PCL. The bromide end groups have been converted into azido end groups, which have been subsequently reduced to the expected primary amine end-capped star-branched PCL by catalytic transfer hydrogenation in DMF. In contrast to living PCL, reaction of trimesic acid chloride with the aluminum alkoxide end group of living poly(L,L)lactide [P(L,L)LA] and living poly(epsilon-caprolactone-b-(L,L)lactide) [P(CL-b-(L,L)LA] chains has produced star-branched polyesters within a very poor yield. This result is more likely due to a steric effect of the methyl group in a-position of the terminal aluminum alkoxide. This drawback has been overcome to some extent by initiating the living polymerization of (L,L)LA with primary amine end-capped star-branched PCL in the presence of triethylaluminum. Formation of a hydroxyl end-capped star-branched poly(epsilon-caprolactone-b-(L,L)lactide) diblock copolymer has accordingly been reported. [less ▲]

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See detailInvestigation of the surface activity of a poly(styrene-b-dimethylsiloxane) copolymer blended within a polystyrene matrix
Petitjean, Serge; Ghitti, G.; Jérôme, Robert ULg et al

in Macromolecules (1994), 27

The surface properties of thin polystyrene films are deeply modified by the addition of various amounts of a poly(styrene-b-dimethylsiloxane) copolymer-or poly(S-b-DMS)-as confirmed by a series of ... [more ▼]

The surface properties of thin polystyrene films are deeply modified by the addition of various amounts of a poly(styrene-b-dimethylsiloxane) copolymer-or poly(S-b-DMS)-as confirmed by a series of analytical methods. In addition to the surface tension which has been estimated from wettability and contact angle measurements, surface composition has been analyzed by X-ray photoelectron spectroscopy (XPS) and secondary ion mass spectrometry (SIMS). Surface studies have been completed by the analysis of the depth distribution of siloxane from the surface by XPS and SIMS. Finally, the surface activity of poly(S-b-DMS) has been investigated in solution. Indeed, changes in the surface tension of polystyrene solutions in THF have been measured in relation to the addition of the diblock copolymer. All the experimental data agree with the complete coverage of the polystyrene films with the poly(S-b-DMS) copolymer. This situation already prevails in the solutions prepared as precursors to the thin polystyrene films. Accordingly, surface characteristics of film freshly prepared by solvent-casting are unaffected by subsequent annealing. [less ▲]

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See detailCold Adaptation of Proteins. Purification, Characterization, and Sequence of the Heat-Labile Subtilisin from the Antarctic Psychrophile Bacillus Ta41
Davail, S.; Feller, Georges ULg; Narinx, E. et al

in Journal of Biological Chemistry (1994), 269(26), 17448-53

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal ... [more ▼]

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal amino acid sequencing of the purified enzyme indicate that the mature subtilisin S41 is composed of 309 residues with a predicted M(r) = 31,224. Subtilisin S41 shares most of its properties with mesophilic subtilisins (structure of the precursor, 52% amino acid sequence identity, alkaline pH optimum, broad specificity, Ca2+ binding) but is characterized by a higher specific activity on macromolecular substrate, by a shift of the optimum of activity toward low temperatures, and by a low thermal stability. The enzyme also differs by an acidic pI (5.3) and the presence of one disulfide bond. It is proposed that the psychrophilic enzyme possesses a more flexible molecular structure when compared to mesophilic and thermophilic subtilases in order to compensate for the reduction of reaction rates at low temperatures. The model of subtilisin S41 indeed reveals several features able to induce a more flexible, heat-labile conformation: the occurrence of four extended surface loops, a very hydrophilic surface through 11 extra Asp residues, and the lack of several salt bridges and aromatic-aromatic interactions. The low affinity of the Ca1 calcium binding site (Kd(app) = 10(-6) M), resulting possibly from one chelating side chain substitution and the stacking of Gly residues, also reflect a less compact conformation. The difference of free energy of stabilization between subtilisin S41 and a mesophilic subtilisin suggests that the balance of exo- and endothermically formed weak bonds is critical for the enzyme flexibility. [less ▲]

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See detailLa chirurgie du carrefour aortique de 1975 à 1991 à Liège
Kolh, Philippe ULg; Dermiens, D.; Marnette, J. M. et al

in Revue Médicale de Liège (1994), 49(7), 397-411

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See detailComment je traite un hypertendu avec insuffisance rénale non diabétique
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(7), 373-374

1. L'hypertension artérielle chez l'insuffisant rénal chronique est fréquente. 2. L'hypertension artérielle accélère, dans bien des situations, la vitesse de dégradation fonctionnelle rénale. 3. Corriger ... [more ▼]

1. L'hypertension artérielle chez l'insuffisant rénal chronique est fréquente. 2. L'hypertension artérielle accélère, dans bien des situations, la vitesse de dégradation fonctionnelle rénale. 3. Corriger l'élévation de pression artérielle ralentit cette progression, en association avec les règles diététiques de régime hyposodé et hypoprotéiné. 4. Les meilleurs bénéfices semblent être obtenus pour des pressions artérielles inférieures à 130/85 mmHg. 5. Les inhibiteurs de l'enzyme de conversion apparaissent plus bénéfiques lorsqu'ils sont prescrits en présence d'une atteinte rénale légère associée à une protéinurie. Leur utilisation nécessite cependant quelques précautions (surveillance des ions et de la fonction rénale). 6. Les antagonistes calciques offrent une grande sécurité d'emploi quel que soit le degré d'atteinte rénale. Cette classe thérapeutique possède des propriétés propices au ralentissement de la dégradation fonctionnelle rénale pour toute néphropathie sans protéinurie. 7. L'association à des diurétiques (surtout de l'anse) est souvent nécessaire, le but étant de normaliser si possible la pression artérielle. [less ▲]

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See detailVariations of the Ap Stars in NGC2169
Manfroid, Jean ULg; Renson, Pierre ULg

in Information Bulletin on Variable Stars (1994), 4044

Not Available

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See detailHubble Space Telescope Goddard high-resolution spectrograph H2 rotational spectra of Jupiter's aurora
Clarke, John T; Ben Jaffel, Lotfi; Vidal-Madjar, Alfred et al

in Astrophysical Journal (1994), 430

We have observed the emission spectrum from Jupiter's north auroral atmosphere with 0.57 A spectral resolution over 1204-1241 A. Bright emissions have been detected from 50 deg to 60 deg latitude at ... [more ▼]

We have observed the emission spectrum from Jupiter's north auroral atmosphere with 0.57 A spectral resolution over 1204-1241 A. Bright emissions have been detected from 50 deg to 60 deg latitude at locations consistent with 6 to 30 R [SUB]J[/SUB] auroral ovals, with much fainter emissions away form the auroral ovals. The emission spectrum is well fitted by both laboratory spectra and theoretical models of optically thin electron excited H2, with added Doppler-broadened Lyman Alpha emission. The observed Lyman Alpha emission wings extend more than 1 A from line center and appear correlated in strength with the H2 brightness. Individual rotational lines in the H2 Werner band system are resolved, allowing a determination of the H2 rotational temperature at the altitude of the emission. We derive best-fit temperatures from 400-450 to 700-750 K, with the auroral emission layer temperature changing either across the auroral oval or over several days' time. These observations demonstrate for the first time the ability to measure the observed rapid H2 temperature variations across Jupiter's auroral atmosphere. [less ▲]

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See detailHow to Measure Insulin Action in Vivo
Scheen, A. J.; Paquot, Nicolas ULg; Castillo, M. J. et al

in Diabetes/Metabolism Reviews (1994), 10(2), 151-88

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See detailRadiation-Induced Myelopathy and Vertebral Necrosis
Martin, Didier ULg; Delacollette, Mireille ULg; Collignon, Jacques ULg et al

in Neuroradiology (1994), 36(5), 405-7

Radiation-induced myelopathy is often a diagnosis of exclusion. In addition to the classic criteria needed to support the diagnosis, the presence of another radiation-induced lesion, such as aseptic ... [more ▼]

Radiation-induced myelopathy is often a diagnosis of exclusion. In addition to the classic criteria needed to support the diagnosis, the presence of another radiation-induced lesion, such as aseptic vertebral necrosis, is useful to confirm the cause of the spinal cord lesion. [less ▲]

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See detailLa Formation de Philippeville à Merlemont: contexte structural, comparaison avec le stratotype et paléoenvironnements
Boulvain, Frédéric ULg; Coen-Aubert, Marie; Dumoulin, Virginie et al

in Professional Paper (1994), 1994/2(269), 1-29921

After considering the dolomite quarry of Merlemont North in its structural and stratigraphic contexts, palaeontological (rugose corals) and sedimentological observations are presented. A ... [more ▼]

After considering the dolomite quarry of Merlemont North in its structural and stratigraphic contexts, palaeontological (rugose corals) and sedimentological observations are presented. A paleoenvironmental reconstruction is proposed and the section is compared to the stratotype of the Philippeville Formation (Belgium, Wallonia, Dinant Synclinorium, Philipeville secondary anticlinorium) [less ▲]

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See detailDepressed Immune Surveillance against Cancer: Role of Deficient T Cell: Extracellular Matrix Interactions
Gorski, A.; Castronovo, Vincenzo ULg; Stepien-Sopniewska, B. et al

in Cell Adhesion and Communication (1994), 2(3), 225-33

Although T cells infiltrate malignant tumors, the local immune response is usually inefficient and tumors escape destruction. While extracellular matrix proteins strongly costimulate T cell responses in ... [more ▼]

Although T cells infiltrate malignant tumors, the local immune response is usually inefficient and tumors escape destruction. While extracellular matrix proteins strongly costimulate T cell responses in normal individuals, our studies indicate that peripheral blood T cells from cancer patients and tumor infiltrating cells respond poorly or are resistant to stimulative signals mediated by collagen I and IV and fibronectin. Moreover, the adhesive properties of cancer T cells are markedly depressed. Those functional deficiencies are paralleled by variable deficits in integrin and non-integrin T cell receptors for extracellular matrix. Immunotherapy with BCG causes a dramatic but transient increase in T cell: ECM interactions. [less ▲]

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See detailComplement, leukocytes, and leukocyte elastase in full-term neonates undergoing cardiac operation.
SEGHAYE, Marie-Christine ULg; Duchateau, J.; Grabitz, R. G. et al

in Journal of Thoracic and Cardiovascular Surgery (The) (1994), 108(1), 29-36

In 13 neonates undergoing cardiac operations for congenital cardiac defects, complement, leukocytes, and leukocyte elastase were studied during and after cardiopulmonary bypass. All but two neonates ... [more ▼]

In 13 neonates undergoing cardiac operations for congenital cardiac defects, complement, leukocytes, and leukocyte elastase were studied during and after cardiopulmonary bypass. All but two neonates received prostaglandin E1 before the operation. The C3d/C3 ratio rose significantly during cardiopulmonary bypass from 0.86 +/- 0.55 to 1.40 +/- 0.56 (mean +/- standard deviation; p < 0.0001). Abnormally elevated C5a levels (18.6 +/- 7.3 micrograms/L) were measured at the end of cardiopulmonary bypass. C4 was not overtly consumed during the procedure. Leukocytes fell from a preoperative value of 10.06 +/- 3.15 x 10(9)/L to 3.21 +/- 0.64 x 10(9)/L after beginning of cardiopulmonary bypass (p < 0.0001) and rose at the end of the procedure from 2.33 +/- 0.67 x 10(9)/L to 7.19 +/- 1.84 x 10(9)/L, after protamine administration (p < 0.0001). Neutrophils fell from a preoperative value of 5.14 +/- 1.18 x 10(9)/L to 1.46 +/- 0.35 x 10(9)/L after beginning of cardiopulmonary bypass and rose at the end of extracorporeal circulation from 1.00 +/- 0.31 x 10(9)/L to 4.10 +/- 1.18 x 10(9)/L, after protamine administration (p < 0.005). Elastase release occurred in all neonates during cardiopulmonary bypass and averaged 331.5 +/- 175.7 micrograms/L. Complement activation and leukocyte stimulation did not correlate with postoperative complications or outcome. This study demonstrates complement activation and leukocyte stimulation in neonates undergoing cardiac operation. [less ▲]

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See detailHigh-field magnetization of U2T2X compounds (T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn)
Nakotte, Heinz; Prokes, Karel; Brück, E. et al

in Physica B: Condensed Matter (1994), 201

Structure parameters and magnetization in quasistatic fields up to 38 T at 4.2 K are reported for U2T2X compounds with T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn, which crystalline in the tetragonal U3Si2 ... [more ▼]

Structure parameters and magnetization in quasistatic fields up to 38 T at 4.2 K are reported for U2T2X compounds with T = Co, Rh, Ni, Pd, Ir, Pt and X = In, Sn, which crystalline in the tetragonal U3Si2 structure. Even the highest applied fields are not sufficient to achieve saturation of the magnetization in any of these compounds of which the majority is antiferromagnetic and some are Pauli paramagnets. The importance of 5f-ligand hybridization for the magnetism in these compounds is discussed. [less ▲]

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See detailImpossible reconversion de l’industrie militaire russe
Bachkatov, Nina ULg

in Monde Diplomatique (Le) : Journal des Cercles Diplomatiques et des Grandes Organisations Internationales (1994)

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See detailIsolation and characterization of preantral follicles from fetal bovine ovaries
Hulshof, S. C. J.; Figueiredo, José Ricardo; Beckers, Jean-François ULg et al

in Veterinary Quarterly (The) (1994), 16(2), 78-80

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The ... [more ▼]

A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter. [less ▲]

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See detailHygiène cutanée
Kort, R.; Nikkels, Arjen ULg; Pierard, Gérald ULg

in Journal d'Actualités Dermatologiques Belges (1994), 12

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See detailAnionic polymerization of acrylic monomers. 14. Carbon-13 and lithium-7 NMR characterization of the complexation of methyl α-lithioisobutyrate with various lithium cation-binding ligands in tetrahydrofuran
Wang, Jin-Shan; Jérôme, Robert ULg; Warin, R. et al

in Macromolecules (1994), 27(12), 3376-3382

The aggregation equilibrium and electronic structure of methyl alpha-lithioisobutyrate (MIBLi), a monomeric model structurally corresponding to a living poly(methyl methacrylate) chain end associated with ... [more ▼]

The aggregation equilibrium and electronic structure of methyl alpha-lithioisobutyrate (MIBLi), a monomeric model structurally corresponding to a living poly(methyl methacrylate) chain end associated with a lithium countercation, has been examined by Li-7 and/or C-13 NMR spectroscopy in tetrahydrofuran (THF) in the presence of various lithium cation-binding ligands. The addition of dimethoxyethane (DME), triglyme (glyme-3),12-crown-4 (12-CE-4), and hexamethylphosphoric triamide (HMPA) to coexisting tetrameric and dimeric MIBLi in THF induces an increase in dimeric population in the order DME < glyme-3 < 12-CE-4 <HMPA. That order corresponds to an increasing strength of complexation between MIBLi (lithium cation) and the (solvating) ligands For either tetrameric or dimeric species, moreover, the negative charge distribution around MIBLi does not seem to be significantly modified due to the presence of these complexing agents. In striking contrast, complexation of MIBLi by cryptand 211 (K211) does shift the equilibrium between tetramer and dimer toward the formation of a single monomeric K211-complexed species, which gives rise to ca. 13 and 5 ppm upfield shifts at C(alpha) in the C-13 NMR spectra, compared to tetrameric and dimeric species, respectively. The effect of 12-CE-4/K211 on the original aggretation equilbrium for MIBLi in THF nicely explains the significantly different MWD of PMMA anionically prepared in the presence of each of these ligands. [less ▲]

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See detailLe diabète de type 2 insulinorequérant: caractéristiques des patients et effets de l'insulinothérapie
Duysinx, Bernard ULg; Scheen, A. J.; Paquot, Nicolas ULg et al

in Revue Médicale de Liège (1994), 49(6), 305-23

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See detailComment je traite un patient hypertendu âgé
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1994), 49(6), 303-304

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées ... [more ▼]

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées à plusieurs reprises et trouvées dépassant les valeurs de 160et/ou 95 mmHg, en raison de la démonstration de l'efficacité certaine de ce traitement dans la diminution de l'incidence des complications cardiovasculaires du patient hypertendu âgé. 2. Le régime sans sel strict doit être évité. Une correction de l'excès de poids doit bien sûr être associée au traitement médicamenteux. 3. Pour le traitement médicamenteux, il est nécessaire de choisir des agents antihypertenseurs dont l'utilisation est bien connue du prescripteur. 4. Ne pas viser d'emblée l'efficacité maximale. Il est conseillé d'augmenter la posologie par palliers successifs. 5. Une surveillance attentive du patient est nécessaire avec recherche des effets secondaires médicamenteux. 6. Si le traitement est inefficace, il est nécessaire de remettre en question ce premier choix. Celui-ci est classiquement un diurétique ou, en cas de contre-indication, un antagoniste calcique. 7. Le schéma thérapeutique doit être facile à comprendre du patient et consister en une prise unique. 8. Rappelons que le but du traitement est de prolonger l'existence du patient tout en assurant une qualité de vie correcte. [less ▲]

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See detailStability and Structural Analysis of Alpha-Amylase from the Antarctic Psychrophile Alteromonas Haloplanctis A23
Feller, Georges ULg; Payan, F.; Theys, F. et al

in European Journal of Biochemistry (1994), 222(2), 441-7

The alpha-amylase secreted by the antarctic bacterium Alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. The psychrophilic alpha-amylase is however ... [more ▼]

The alpha-amylase secreted by the antarctic bacterium Alteromonas haloplanctis displays 66% amino acid sequence similarity with porcine pancreatic alpha-amylase. The psychrophilic alpha-amylase is however characterized by a sevenfold higher kcat and kcat/Km values at 4 degrees C and a lower conformational stability estimated as 10 kJ.mol-1 with respect to the porcine enzyme. It is proposed that both properties arise from an increase in molecular flexibility required to compensate for the reduction of reaction rates at low temperatures. This is supported by the fast denaturation rates induced by temperature, urea or guanidinium chloride and by the shift towards low temperatures of the apparent optimal temperature of activity. When compared with the known three-dimensional structure of porcine pancreatic alpha-amylase, homology modelling of the psychrophilic alpha-amylase reveals several features which may be assumed to be responsible for a more flexible, heat-labile conformation: the lack of several surface salt bridges in the (beta/alpha)8 domain, the reduction of the number of weakly polar interactions involving an aromatic side chain, a lower hydrophobicity associated with the increased flexibility index of amino acids forming the hydrophobic clusters and by substitutions of proline for alanine residues in loops connecting secondary structures. The weaker affinity of the enzyme for Ca2+ (Kd = 44 nM) and for Cl- (Kd = 1.2 mM at 4 degrees C) can result from single amino acid substitutions in the Ca(2+)-binding and Cl(-)-binding sites and can also affect the compactness of alpha-amylase. [less ▲]

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See detailThe use of gravitational microlensing to scan the structure of BAL QSOs
Hutsemekers, Damien ULg; Surdej, Jean ULg; van Drom, E.

in Astrophysics & Space Science (1994), 216

Approximately 10% of the quasi-stellar objects (QSOs) show broad absorption lines (BAL) in their spectra which, if interpreted in terms of Doppler velocities, reveal the presence of high velocity gas ... [more ▼]

Approximately 10% of the quasi-stellar objects (QSOs) show broad absorption lines (BAL) in their spectra which, if interpreted in terms of Doppler velocities, reveal the presence of high velocity gas outflows. One of these BAL QSOs is known to be gravitationally lensed. It therefore constitutes a good candidate to search for microlensing effects, i.e., the selective amplification of different line forming regions. Considering current models for the BAL region, we have investigated the effects of moving microlenses on the line profiles, and we conclude that these effects strongly depend on the adopted model. A regular spectroscopic monitoring of lensed BAL QSOs would therefore be highly valuable to distinguish between the various models proposed so far to interpret the origin of broad absorption lines. [less ▲]

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See detailExpression of Bone Sialoprotein, a Bone Matrix Protein, in Human Breast Cancer
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Castronovo, Vincenzo ULg

in Cancer Research (1994), 54(11), 2823-6

Microcalcifications are often associated with human mammary lesions, particularly with breast carcinomas. To date, the molecular mechanism that leads to the deposition of hydroxyapatite in the mammary ... [more ▼]

Microcalcifications are often associated with human mammary lesions, particularly with breast carcinomas. To date, the molecular mechanism that leads to the deposition of hydroxyapatite in the mammary tissue has not been elucidated. Bone sialoprotein (BSP) is a glycoprotein the expression of which coincides with the appearance of the first hydroxyapatite crystals during bone development. In this study, we report the observation that BSP, a bone matrix protein, is expressed in human mammary cancer cells. Using an immunoperoxidase technique, we studied the expression of BSP in 79 breast lesions, including 28 benign and 51 malignant specimens. Two polyclonal antibodies, one directed against intact human BSP and the other against a synthetic peptide of BSP (residues 277-294), were used and gave identical results. Normal mammary glands expressed undetectable or barely detectable amounts of BSP, and the majority of the benign lesions examined were generally unstained (0) or weakly stained (1+). Most of the breast carcinoma specimens (around 87%) showed a significant increase (P = 0.0001) in BSP expression. Breast carcinomas with microcalcifications had the highest immunoreactivity (2+ or 3+) to BSP antibodies. This is the first demonstration that BSP expression is significantly increased in breast cancer. Expression of BSP by breast cancer cells could play a major role in the deposition of microcalcifications and in the preferred bone homing of breast cancer cells. [less ▲]

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See detailNumerical Procedure for Calculation of Temperature Evolution in Composite Elements with Internal Cavities
Luycks, Pierre; Stienon, P.; Franssen, Jean-Marc ULg et al

in Communications in Numerical Methods in Engineering (1994), 10(6), 437-451

In the paper a computation code based on the finite-element method for temperature evolutions in composite elements with internal cavities is presented. It is suitable for the analysis of various thermal ... [more ▼]

In the paper a computation code based on the finite-element method for temperature evolutions in composite elements with internal cavities is presented. It is suitable for the analysis of various thermal problems encountered in mechanical and civil engineering such as fire risk assessment. It is based on a plane isoparametric triangular element with six nodes allowing the modelling of curved boundaries. In order to analyze composite steel-concrete elements and to take into account the non-linearities coming from the temperature-dependent material properties and from the boundary conditions, an implicit integration scheme is used. Another advantage of the program is the possibility of describing internal cavities with convection and radiation exchanges. In order to show the capacity of the program two examples are presented. Comparisons have been made with experimental and other numerical results, exhibiting very good agreement. [less ▲]

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See detailChiral Separation of Basic Drugs by Capillary Zone Electrophoresis with Cyclodextrin Additives
Bechet, I.; Paques, P.; Fillet, Marianne ULg et al

in Electrophoresis (1994), 15(6), 818-23

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and ... [more ▼]

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and uncoated fused silica capillaries thermostated at 15 degrees C. The nature of the cationic component of the background electrolyte was found to have a significant influence on achiral resolution and peak symmetry. The best results were obtained with triethanolamine, which was then used to adjust the buffer pH in all further experiments. The effects on chiral resolution of the nature and concentration of cyclodextrin, of the addition of methanol, and of capillary temperature were studied. Maximum resolution was obtained at a particular cyclodextrin concentration for each analyte, depending on the affinity of the analyte for this cyclodextrin. On the basis of the results, the effects of methanol addition and temperature on enantiomeric resolution could be explained and predicted. Numerous chiral separations are presented and suggestions for the rapid optimization of CZE enantioseparations with cyclodextrin additives are given. [less ▲]

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See detailAcquisition of new concepts by two amnesic patients
Van der Linden, Martial ULg; Meulemans, Thierry ULg; Lorrain, D.

in Cortex : A Journal Devoted to the Study of the Nervous System & Behavior (1994), 30(2), 305-317

Two Korsakoff amnesics (A.G. and G.S.) and two control subjects were taught six new concepts. Each concept was composed of three parts: the name of the concept, the context in which the concept originated ... [more ▼]

Two Korsakoff amnesics (A.G. and G.S.) and two control subjects were taught six new concepts. Each concept was composed of three parts: the name of the concept, the context in which the concept originated and its definition. The learning procedure consisted of two phases: (1) learning the concept names and definitions by means of the vanishing-cues method; (2) practice on examples of the concepts through a classification task: examples were either set in the same context as that given in the original definition or in mixed contexts (same and new contexts). Subjects were then tested after 24 hours, a week and a month on their ability to identify new examples as belonging to one of the conceptual rules studied (transfer tests). Both patients showed substantial learning. Patient A.G. was slow and dependent of the first letter cues in the vanishing-cues learning phase but nevertheless, she acquired a large and flexible conceptual knowledge and this was especially true for concepts that were practised by means of mixed-context examples. Patient G.S. easily learned to associate the definitions with the concept names but her conceptual knowledge remained more limited. These results confirm the existence of a semantic learning ability in amnesic patients. They also suggest that under appropriate learning conditions, amnesics may eventually acquire a new flexible conceptual knowledge. [less ▲]

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See detailSchimmeldiagnostiek: Nieuwe technieken
Pierard, Gérald ULg; Nikkels, Arjen ULg

in Janssen Medisch-Wetenschappelijk Nieuws (1994), 9

De diagnose schimmelinfectie van de huid of de nagel kan bevestigd worden met een KOH-preparaat (microscopie) of met een kweek. Beide methoden kunnen een vals-negatieve of vals-positieve iutslag hebben ... [more ▼]

De diagnose schimmelinfectie van de huid of de nagel kan bevestigd worden met een KOH-preparaat (microscopie) of met een kweek. Beide methoden kunnen een vals-negatieve of vals-positieve iutslag hebben. Nieuwe techniken - immunohistochemie en flow-cytometrue - kunnen een aanvulling vormen op de bestaande methoden. Vooral voor de identificatie van schimmels kunnen deze methoden waardevol zijn. Voorlopig zijn deze technieken alleen in gebruik voor wetenschappelijk onderzoek. Ze leveren een belangrijke bijdrage aan de kennis over de etiologie van schimmelinfecties. Zo komt er steeds meer bewijs dat nagelinfecties lang niet altijd worden veroorzaakt door dermatofyten, maar dat ook gisten en overige schimmels pathogeen kunnen zijn voor de nagel. [less ▲]

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See detailLa aricultura en el espacio rural europeo
Mormont, Marc ULg

in Agricultura y Sociedad (1994), (No. 71), 17-49

The current crisis in EU agriculture, which is being forced to develop new structures to contend with international competition, could be seen as an entirely economic sectoral crisis. However, the ... [more ▼]

The current crisis in EU agriculture, which is being forced to develop new structures to contend with international competition, could be seen as an entirely economic sectoral crisis. However, the repercussions are in fact widespread, resulting in social change and a profound identity crisis. This article examines the relationship between agriculture and the rural sector, and shows that many traditional agricultural/rural links have been broken due to the economic and commercial development of agriculture, leading to increasing marginalization of many rural areas and extensive rural depopulation. The effects on the environment are also considered. It is concluded that rural areas must become areas to live in, and rather than being dependent on agriculture they must develop in harmony with it. [less ▲]

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See detailEnseigner en 1994, est-ce une mission impossible ?
Beckers, Jacqueline ULg

in Education-Formation (1994)

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See detailInfective Endocarditis: Prevention, Diagnosis and Management
Pierard, Luc ULg; LANCELLOTTI, Patrizio ULg; Galiuto, L.

in European Journal of Emergency Medicine (1994), 1(2), 104-9

Infective endocarditis remains an important problem and the means of prevention are still insufficient. The causal bacteria have changed very little, but the incidence of nosocomial infections and ... [more ▼]

Infective endocarditis remains an important problem and the means of prevention are still insufficient. The causal bacteria have changed very little, but the incidence of nosocomial infections and endocarditis complicating intravenous drug abuse are increasing. The distinction between subacute and acute clinical presentations remains appropriate. Cardiac and neurological complications are frequent and carry a high risk of mortality. The diagnosis is obtained by the integration of clinical data and the results of blood cultures. Echocardiography is extremely useful for detecting vegetations, and for assessing the haemodynamic consequences and specific cardiac complications. Risk stratification can be obtained by correct integration of multiple parameters. The causal agent should be identified before the initiation of antimicrobial therapy. Surgery is frequently required, and should be performed rapidly when indicated. [less ▲]

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See detailAfferent and Efferent Connections of the Sexually Dimorphic Medial Preoptic Nucleus of the Male Quail Revealed by in Vitro Transport of Dii
Balthazart, Jacques ULg; Dupiereux, V.; Aste, N. et al

in Cell & Tissue Research (1994), 276(3), 455-75

The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this ... [more ▼]

The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this nucleus in the control of reproduction requires the identification of its afferent and efferent connections. In order to identify neural circuits involved in the control of the medial preoptic nucleus, we used the lipophilic fluorescent tracer DiI implanted in aldheyde-fixed tissue. Different strategies of brain dissection and different implantation sites were used to establish and confirm afferent and efferent connections of the nucleus. Anterograde projections reached the tuberal hypothalamus, the area ventralis of Tsai, and the substantia grisea centralis. Dense networks of fluorescent fibers were also seen in several hypothalamic nuclei, such as the anterior medialis hypothalami, the paraventricularis magnocellularis, and the ventromedialis hypothalami. A major projection in the dorsal direction was also observed from the medial preoptic nucleus toward the nucleus septalis lateralis and medialis. Afferents to the nucleus were seen from all these regions. Implantation of DiI into the substantia grisea centralis also revealed massive bidirectional connections with a large number of more caudal mesencephalic and pontine structures. The substantia grisea centralis therefore appears to be an important center connecting anterior levels of the brain to brain-stem nuclei that may be involved in the control of male copulatory behavior. [less ▲]

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See detailThe Er-Tr4 Monoclonal Antibody Recognizes Murine Thymic Epithelial Cells (Type 1) and Inhibits Their Capacity to Interact with Immature Thymocytes: Immuno-Electron Microscopic and Functional Studies
Defresne, Marie-Paule ULg; Nabarra, B.; van Vliet, E. et al

in Histochemistry (1994), 101(5), 355-63

The thymic stroma is heterogeneous with regard to cellular morphology and cellular function. In this study, we employed the monoclonal antibody ER-TR4 to characterize stromal cells at the ultrastructural ... [more ▼]

The thymic stroma is heterogeneous with regard to cellular morphology and cellular function. In this study, we employed the monoclonal antibody ER-TR4 to characterize stromal cells at the ultrastructural level. To identify the labelled cell type, we used two techniques: immunogold labelling on ultrathin frozen sections and immunoperoxidase staining on thick "vibratome" sections. ER-TR4 reacted with thymic Type 1 epithelial cells (according to our classification). A dense labelling appears in the cytoplasm of cortical cells using the two techniques. Immunogold labelling identified small cytoplasmic vesicles whereas the cytoplasm and the cell membrane seem to be labelled with the immunoperoxidase technique. ER-TR4 also identified isolated thymic nurse cells (TNC), and was observed in vitro to inhibit the capacity of some type 1 epithelial cells to establish interactions with immature thymocytes. This finding supports the hypothesis that the factor is involved in the formation of lymphoepithelial interactions within thymic nurse cells, and thus in the relations that immature thymocytes establish with the thymic microenvironment. [less ▲]

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See detailInverse Expression of Two Laminin Binding Proteins, 67lr and Galectin-3, Correlates with the Invasive Phenotype of Trophoblastic Tissue
van den Brule, F. A.; Price, J.; Sobel, M. E. et al

in Biochemical and Biophysical Research Communications (1994), 201(1), 388-93

Tumor invasion of host tissues and trophoblastic penetration of the endometrium share common biological features. Both processes involve the invasion of basement membranes, an event that is initiated by ... [more ▼]

Tumor invasion of host tissues and trophoblastic penetration of the endometrium share common biological features. Both processes involve the invasion of basement membranes, an event that is initiated by adhesion of cancer or trophoblast cells to basement membrane components and particularly to laminin. Adhesion to this latter glycoprotein is mediated through a variety of cell surface receptors. We have previously shown that the 67 kD Laminin Receptor (67LR) and a 31 kD Human Laminin Binding Protein, recently renamed galectin-3, are inversely modulated as the invasive phenotype of cancer cells progresses, with up regulation of the former, and down regulation of the latter, respectively. In this study, we examined the expression of these two proteins in 27 human trophoblastic specimens at different gestational ages using Northern and Western blot techniques. Expression of the 67LR increased from 7 weeks to a maximum at 12 weeks, when invasion is maximal, and then decreased. Expression of galectin-3 was inversely modulated by the gestational age, with a minimum expression at 12 weeks. Our data demonstrate that invasive trophoblast displays the same pattern of laminin binding proteins expression than invasive cancer cells, and further demonstrates that invasion of the extracellular matrix by trophoblast and cancer cells share common molecular mechanisms. [less ▲]

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See detailTestosterone Effects on Neurotensin-Immunoreactive Cells in the Quail Preoptic Area
Absil, Philippe ULg; Balthazart, Jacques ULg

in Neuroreport (1994), 5(9), 1129-32

In the quail preoptic area (POA), neurotensin-immunoreactive (NT-ir) cells are mostly located in the sexually dimorphic medial preoptic nucleus (POM). They are more numerous in females than in males and ... [more ▼]

In the quail preoptic area (POA), neurotensin-immunoreactive (NT-ir) cells are mostly located in the sexually dimorphic medial preoptic nucleus (POM). They are more numerous in females than in males and, in females, their number varies during the ovulatory cycle. This suggests a control by steroids of NT expression in the POA. This idea was tested by comparing the NT-ir populations in gonadectomized males and females treated or not with testosterone. No sex differences in the NT-ir cell number was present in gonadectomized birds. Testosterone treatment increased the number of immunoreactive perikarya. This effect was anatomically specific and the increase was observed in the area caudal to the anterior commissure but not in POM itself. [less ▲]

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See detailPrimary cerebral lymphoma. A retrospective study of 27 cases
Grangier, C; COUCKE, Philippe ULg; Baur, A et al

in Strahlentherapie und Onkologie (1994), 170(4), 206-212

Primary cerebral lymphoma is a rare disease. The aim of this study is to compare the survival of the patients treated with radiotherapy alone vs. patients treated with a combined schedule of radio ... [more ▼]

Primary cerebral lymphoma is a rare disease. The aim of this study is to compare the survival of the patients treated with radiotherapy alone vs. patients treated with a combined schedule of radio-chemotherapy. Our results will be compared with currently published data and main prognostic factors will be briefly discussed. PATIENTS AND METHODS: Between 1974 and 1990, 27 cases of primary cerebral lymphoma were diagnosed at our institution. All patients had biopsy-proven disease, the pathology of which was reviewed for this study. RESULTS: The overall median survival time was 24 months and one-, two- and three-year overall survival was 59, 46 and 29% respectively. The median radiation dose was 46 Gy, ranging from 19.5 to 60 Gy. The median dose per fraction was 2 Gy (ranging from 1.61 to 3 Gy). The median elapsed treatment time was 32 days (ranging from three to 45 days). We were not able to demonstrate any statistically significant difference between patients who received radiotherapy alone (n = 14, median survival time = 24 months) and those who received a combination of chemotherapy and radiotherapy (n = 11, median survival time = 30 months), (p = 0.4). Prognostic factors of survival were tested using a univariate analysis (Wilcoxon test). Parameters such as mass appearance (unilobular, p = 0.048), performance status at the time of the diagnosis (0 to 1, p = 0.014), and CT imaging (hypodense, p = 0.043) influenced positively survival. Centroblastic histology (Kiel) was found associated with a negative prognosis (p = 0.043). CONCLUSION: In our experience, there is no statistically significant difference of survival between patients treated with radiotherapy alone or with a combined treatment of radio-chemotherapy. Other prognostic factors of survival were discovered, although the analysis was univariate, due to the limited number of patients. Multicentric prospective studies should be elaborated in order to optimize the treatment of this disease. [less ▲]

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See detailCalcitonine en prévention et traitement de l'ostéoporose
Reginster, Jean-Yves ULg

in Revue Médicale de Liège (1994), 49(5), 253-9

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See detailPeriod of the Si-star in NGC 2169
Renson, Pierre ULg

in Astronomy and Astrophysics (1994), 285

For the Si Ap-star known in NGC 2169 (Gr.9 in the cluster), a period between 0.60 and 0.62 d, most likely about 0.606 d, agrees with all known observations, and is much more probable than the period 1.56 ... [more ▼]

For the Si Ap-star known in NGC 2169 (Gr.9 in the cluster), a period between 0.60 and 0.62 d, most likely about 0.606 d, agrees with all known observations, and is much more probable than the period 1.56 d proposed by Maitzen & Lebzelter (1993). The obtained period is among the shortest ones known for Ap stars. [less ▲]

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See detailLa protéine P53, protectrice de l'intégrité du génome: rôle dans la genèse des cancers
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Gielen Jacques et al

in Revue Médicale de Liège (1994), 49(5), 274-84

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et ... [more ▼]

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et déterminent leur appartenance tissulaire. Des données récentes indiquent que l'apparition des cancers résulte du déséquilibre de cet état dynamique soit part l'activation de facteurs positifs désignés sous le terme d'oncogènes, soit par l'inactivation de facteurs négatifs qui sont regroupés dans la famille des gènes suppresseurs de tumeurs. Le gène codant pour la protéine p53 est un membre particulièrement important de cette dernière famille. En effet, son inactivation, par mutation et/ou délétion, est l'une des altérations génétiques la plus fréquemment détectée dans les cancers. La fonction principale attribuée à la protéine p53 consiste à préserver le génome des altérations susceptibles d'entraîner, entre autres, la cellule dans un processus de transformation maligne. C'est en permettant la réparation des altérations du DNA survenues lors de sa réplication avant la mitose ou provoquées par des agents extérieurs, que la protéine p53 semble exercer ses fonctions. Des altérations du gène p53 entraînant des perturbations de la fonction de la protéine p53 ont été identifiées au niveau de la plupart des lésions tumorales malignes. A ce titre, cette protéine semble jouer un rôle déterminant au niveau de la genèse des cancers. Aussi le gène p53 fait-il l'objet de recherches intensives qui devraient déboucher sur la mise au point de nouveaux moyens de détection et d'évaluation pronostique des cancers. D'autre part, des expériences visant à restaurer la fonction de la protéine p53 au niveau des cellules cancéreuses ouvrent de nouvelles et séduisantes perspectives thérapeutiques. [less ▲]

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See detailEfficacy and Tolerability of a New Formulation of Oral Tiludronate (Tablet) in the Treatment of Paget's Disease of Bone
Reginster, Jean-Yves ULg; Treves, R.; Renier, J. C. et al

in Journal of Bone and Mineral Research : The Official Journal of the American Society for Bone and Mineral Research (1994), 9(5), 615-9

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial ... [more ▼]

We sought to assess efficacy and safety of a new oral formulation (tablet) of tiludronate in Paget's disease of bone. We studied 128 patients with Paget's disease in an open-label uncontrolled trial. Patients received a daily dose of 400 mg oral tiludronate (two tablets). Treatment was for 6 months. Serum alkaline phosphatase activity (SAP) and fasting urinary excretion of hydroxyproline/creatine (OH/Cr) were measured every 3 months, as were biochemical parameters reflecting renal, hepatic, and hematologic functions. Analgesic efficacy was self-evaluated from a visual analog scale (VAS). Statistical analysis revealed a significant reduction from baseline in SAP and OH/Cr levels, as well as VAS scores. In the whole population with evaluation under treatment, there was a reduction in initial SAP activity after 3 months (47.2 +/- 2.2%, mean +/- SEM) and 6 months (58.3 +/- 2.3%). In the population with SAP levels above twice the upper limit at inclusion and with evaluation at month 3 and month 6 (n = 96), the reduction in SAP levels was 49.3 +/- 2.4% after 3 months and of 59.5 +/- 2.6% after 6 months (ANOVA time effect, p = 0.0001). Aside from mild gastrointestinal disturbances, as experienced with other oral bisphosphonates, clinical tolerance was good. Exhaustive biochemical investigation failed to reveal significant toxicity of tiludronate tablets at the dose of 400 mg/day. The dose of 400 mg daily of this new formulation appears to be a satisfactory tiludronate regimen for the treatment of Paget's disease of bone. [less ▲]

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See detailMacrophages and Tumor Necrosis Factor Alpha in Toxic Epidermal Necrolysis
Paquet, Philippe ULg; Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg et al

in Archives of Dermatology (1994), 130(5), 605-608

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell ... [more ▼]

BACKGROUND: We studied the immunopathologic characteristics of five cases of toxic epidermal necrolysis by using a large panel of antibodies. OBSERVATIONS: The pattern and amount of the inflammatory cell infiltrate varied according to the stage of the disease. The main constant feature was the prominent involvement of the monocyte-macrophage lineage, including factor XIIIa+HLA-DR+ dendrocytes and CD68+ Mac 387+ macrophages, before and during the epidermal necrosis. The number of CD4+ and CD8+ lymphocytes was comparatively small. This was associated with a dense labeling of the epidermis for tumor necrosis factor alpha. CONCLUSIONS: Cells of the monocyte-macrophage lineage largely outnumber lymphocytes in the lesions of toxic epidermal necrolysis. Tumor necrosis factor alpha is likely a major cytokine that is responsible for necrosis. [less ▲]

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See detailDirect NMR evidence for a local resriction in the segmental chain mobility of a model ionomer
Vanhoorne, Pierre; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(9), 2548-2552

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which ... [more ▼]

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which the chain segments bearing the sodium carboxylate end groups have been selectively protonated; the second one has a protonated polystyrene backbone terminated at both ends with a deuterated polystyrene segment bearing the sodium carboxylate groups. Both polymers have been studied by high-resolution solid-state C-13 NMR. Line-width measurements of the protonated units have clearly shown that the dipolar interactions of the sodium carboxylate end groups are responsible for a mobility restriction of the chain end segments. This experiment is thought to be the first direct evidence for the model recently proposed by Eisenberg et al. for ionomer morphology according to which the ionic multiplets are surrounded with a shell of constrained polymer segments. [less ▲]

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See detailAgm-1470, a Potent Angiogenesis Inhibitor, Prevents the Entry of Normal but Not Transformed Endothelial Cells into the G1 Phase of the Cell Cycle
Antoine, Nadine ULg; Greimers, Roland ULg; De Roanne, C. et al

in Cancer Research (1994), 54(8), 2073-6

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this ... [more ▼]

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this molecule appears to be a most promising anticancer drug, its mechanism of action has not yet been elucidated. In this study, we examined the effects of AGM-1470 on the cell cycle of normal and transformed endothelial cells. We showed that AGM-1470, at picomolar concentrations, specifically inhibits the proliferation of both bovine aortic endothelial cells and human umbilical vein endothelial cells. AGM-1470 was ineffective in significantly inhibiting the proliferation of Ea.hy926 cells, a hybrid cell line obtained by the fusion of human umbilical vein endothelial cells with a human carcinoma cell line, or cEnd.1 cells, a polyoma middle T oncogene-transformed endothelioma cell line derived from mouse embryo. Using a double labeling technique with anti-Ki67 antibodies and propidium iodide, we demonstrated, with flow cytometry analysis, that AGM-1470 specifically prevents the entry of endothelial cells into the G1 phase of the cell cycle. We also showed that AGM-1470 was ineffective in inhibiting endothelial cell migration toward laminin or capillary-like tube formation inside a type I collagen matrix induced by phorbol esters. Our data strongly suggest that AGM-1470 is a molecule that specifically inhibits a cell cycle control pathway active in normal cells but which could be bypassed or altered in transformed cells. [less ▲]

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See detail4 YEARS RESEARCH ON THE REPRODUCTIVE-BEHAVIOR AND HYBRIDIZATION OF BARBUS-BARBUS AND BARBUS-MERIDIONALIS
Poncin, Pascal ULg

in Bulletin Français de la Pêche et de la Pisciculture (1994), 334

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The ... [more ▼]

This paper reviews the main results of 4 years studies on the reproductive behaviour of Barbus barbus (L., 1758) and Barbus meridionalis (Risso, 1826) under controlled conditions in aquarium. The reproductive behaviour is described as well as the behavioural mechanisms of hybridization. During the spawning act - sometimes preceded by <<forehead swim>> in which two fish swim together, at high speed, head against head the female and the few accompanying males rise from the gravel and move their genital papilla in the gravel while releasing ova or sperm (fig. 1 and 2). The reproductive behaviours of Barbus barbus and Barbus meridionalis appear to be similar and hybridizations were observed, showing that no complete behavioural barrier blocks the mating of the two species (fig. 2). The daily rhythms of activity are compared within the two species. The influence of temperature and daylength on the daily rhythms of activity is discussed. in winter conditions, barbels exhibit a night activity rhythm. Spawning occurs during daytime or during the night (fig. 3). The influence of spawning stimuli is analysed using dummies of different sizes and colors (photo I). Barbels seem to prefer white, grey, yellow and brown colors to red or black colors. [less ▲]

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See detailKnowledge-Based System for the Automated Solid-Phase Extraction of Basic Drugs from Plasma Coupled with Their Liquid Chromatographic Determination. Application to the Biodetermination of Beta-Receptor Blocking Agents
Hubert, Philippe ULg; Chiap, Patrice ULg; Moors, M. et al

in Journal of Chromatography. A (1994), 665(1), 87-99

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges ... [more ▼]

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges (DECs) by means of a sample processor. A knowledge-based system was developed to facilitate the development of fully automated methods for the solid-phase extraction of relatively hydrophobic basic drugs from plasma, coupled with their determination by high-performance liquid chromatography (HPLC). The DEC filled with 50 mg of cyanopropyl-bonded silica phase is first conditioned with methanol and buffer solution (pH 7.4). After sample application, the DEC sorbent is washed with the same buffer. The analytes are then desorbed with an appropriate eluent and the eluate is finally diluted with the same buffer as used in the HPLC mobile phase before injection. Under these conditions, only three variables are still to be optimized: the composition and volume of the elution solvent and the volume of buffer to be added to the eluate. On the basis of this general strategy, a decision tree providing information about suggested starting conditions and guidelines for the optimization of the three variables was developed and implemented by use of a hypermedia software. This didactic expert system was evaluated using several beta-receptor blocking agents as model compounds and the operating conditions obtained for the automated SPE of these compounds are presented. A method for the determination of propranolol in plasma using the SPE conditions deduced from the knowledge-based system was validated. The absolute recovery of propranolol is ca. 93% and the limit of detection is 1.3 ng ml-1. The mean within-day and between-day reproducibilities are 2.3 and 3.6%, respectively. [less ▲]

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See detailIncreased Parasellar Activity on Gallium Spect Is Not Specific for Active Cluster Headache
Sianard-Gainko, J.; Milet, J.; Ghuysen, V. et al

in Cephalalgia : An International Journal of Headache (1994), 14(2), 132-3

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients ... [more ▼]

We have performed Gallium SPECT head scans in 30 successive cluster headache (CH) patients and in 7 migraineurs without aura. Parasellar hyperactivity was judged as present in 81% of chronic CH patients, 54% of episodic CH patients in an active period, 56% of episodic CH patients in remission and 71% of migraineurs. No significant correlations were found between the SPECT images and the duration of the disease, of cluster periods or of remissions. Increased parasellar activity on Gallium SPECT is thus not specific for CH, nor for the active period of episodic CH. The method lacks reliability for investigation of putative cavernous sinus inflammation. [less ▲]

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