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See detailAnisotropic AC behavior of multifilamentary Bi-2223/Ag tapes
Fagnard, Jean-François ULiege; Vanderbemden, Philippe ULiege; Cloots, Rudi ULiege et al

in IEEE Transactions on Applied Superconductivity (2003), 13(2, Part 3), 2976-2979

In this communication, we report on the anisotropy of the superconducting properties of multifilamentary Bi-based tapes experimentally investigated by AC magnetic susceptibility measurements. The ... [more ▼]

In this communication, we report on the anisotropy of the superconducting properties of multifilamentary Bi-based tapes experimentally investigated by AC magnetic susceptibility measurements. The susceptibility X = X' - jX" was measured using a commercial system and a couple of orthogonal pick-up coils. The X'' vs. temperature curves were shown to exhibit two peaks. The smaller of the peaks, occurring near T = 72 K, was only visible for particular field directions and within a given frequency window. Such results point out the role played by the phase difference between the applied magnetic field and the internal magnetic field seen by the filaments. (C) 2003 IEEE. [less ▲]

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See detailThe clinical implications of the systemic inflammatory reaction related to cardiac operations in children.
SEGHAYE, Marie-Christine ULiege

in Cardiology in the Young (2003), 13(3), 228-39

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See detailCeramic Journal Bearing in Cryogenic Turbo-Pumps
Nelis, M.; Bozet, Jean-Luc ULiege; BICKEL, M. et al

in European journal of mechanical and environmental engineering (2003), 48(no2), 73-80

The availability of components built in CMC technology forms in major areas a prerequisite for the realisation of future reusable launcher engines. Apart from the efforts to introduce CMC components in ... [more ▼]

The availability of components built in CMC technology forms in major areas a prerequisite for the realisation of future reusable launcher engines. Apart from the efforts to introduce CMC components in the hot areas of the engine like nozzles and thrust chambers there exists an important optimisation potential in the engines through the use of CMC components in (cryogenic) turbo-pumps. By replacement of the ball bearings in the pumps by journal bearings, a significantly higher lifetime can be obtained and the inherent limitation of the turning speed of the pumps can be widely extended. The CMC journal bearings of MAN have proven their quality and reliability in numerous applications in industrial pumps. Based on this experience a research program was initiated by DLR and funded by means of the BMBF and MAN to investigate the use of this bearing type under the conditions of a cryogenic turbo-pump. Major results are presented in this paper. [less ▲]

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See detailOwnership vs. Accountability, ou le contrôle des fonds de l’aide au développement
Paul, Elisabeth ULiege

in Reflets et Perspectives de la Vie économique (2003), XLII(2), 29-43

The paper reviews the major changes observed in the mechanisms used to channel aid to developing countries. As the most recent PRGF approach places the emphasis on the need for a coherent framework (the ... [more ▼]

The paper reviews the major changes observed in the mechanisms used to channel aid to developing countries. As the most recent PRGF approach places the emphasis on the need for a coherent framework (the PRSP), a certain number of prerequisite on the management side of public expenditure need to be met. The paper reviews some of the most important aspects, in particular the need for better controls in the budgetary process. The paper uses a principal-agent approach to compare the impact of internal and external (audit) controls on the efficiency of service delivery and draws policy implications. [less ▲]

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See detailLa vaccination a base de mycobacteries: vaincra-t-elle les allergies?
Louis, Renaud ULiege

in Revue Médicale de Liège (2003), 58(6), 392-5

In developed countries, the prevalence of tuberculosis has evolved in an opposite direction as to the one of allergy over the last century. The immunological response is mainly Th1 in tuberculosis while ... [more ▼]

In developed countries, the prevalence of tuberculosis has evolved in an opposite direction as to the one of allergy over the last century. The immunological response is mainly Th1 in tuberculosis while it features a Th2 pattern in allergy. Vaccination with BCG in early life is associated with a reduction in the prevalence of allergy later in childhood. In an experimental mouse model of asthma, administration of BCG or killed Mycobacterium vaccae inhibits the sensitisation process as well as the bronchial inflammation and hyperresponsiveness that follows allergen exposure. In children and adolescents suffering from atopic dermatitis, subcutaneous injection of killed Mycobacterium vaccae attenuates the severity of skin lesions. [less ▲]

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See detailThe kinetic properties of the carboxy terminal domain of the Bacillus licheniformis 749/I BlaR penicillin-receptor shed a new light on the derepression of beta-lactamase synthesis
Duval, Valérie; Swinnen, Marc; Lepage, Sophie et al

in Molecular Microbiology (2003), 48(6), 1553-1564

To study the properties of the BlaR penicillin-receptor involved in the induction of the Bacillus licheniformis beta-lactamase, the water-soluble carboxy terminal domain of the protein (BlaR-CTD) was ... [more ▼]

To study the properties of the BlaR penicillin-receptor involved in the induction of the Bacillus licheniformis beta-lactamase, the water-soluble carboxy terminal domain of the protein (BlaR-CTD) was overproduced in the periplasm of Escherichia coli JM105 and purified to protein homogeneity. Its interactions with various beta-lactam antibiotics were studied. The second-order acylation rate constants k(2)/K' ranged from 0.0017 to more than 1 muM(-1) s(-1) and the deacylation rate constants were lower than 4x10(-5) s(-1) . These values imply a rapid to very rapid formation of a stable acylated adduct. BlaR-CTD is thus one of the most sensitive penicillin-binding proteins presently described. In the light of these results, the kinetics of beta-lactamase induction in Bacillus licheniformis were re-examined. When starting with a rather high cell density, a good beta-lactamase substrate such as benzylpenicillin is too sensitive to beta-lactamase-mediated hydrolysis to allow full induction. By contrast, a poor beta-lactamase substrate (7-aminocephalosporanic acid) can fully derepress beta-lactamase expression under conditions where interference of the antibiotic with cell growth is observed. These results suggest that acylation of the penicillin receptor is a necessary, but not sufficient, condition for full induction. [less ▲]

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See detailPrevention du paludisme chez l'adulte
Leonard, Philippe ULiege; Moutschen, Michel ULiege; Demonty, Jean ULiege

in Revue Médicale de Liège (2003), 58(6), 382-7

Great scourge of poor countries, malaria is the most important tropical parasitic disease. It is responsible for a large number of deaths in concerned countries and represents a real danger for travellers ... [more ▼]

Great scourge of poor countries, malaria is the most important tropical parasitic disease. It is responsible for a large number of deaths in concerned countries and represents a real danger for travellers going to endemic regions. So, prophylactic measures are essential. On the one hand protective measures against mosquito bites, by wearing covering clothes, by using repellents and bed net (eventually impregnated with insecticide) will be useful. On the other hand, chemoprophylaxis is most often necessary, adapted to the possibility of chloroquine resistant P. falciparum, to the length or conditions of travel, and to the traveller's antecedents and age. Special concern about pregnant woman is necessary, due to potential severity of malaria. Chemoprophylaxis needs to be continued after coming back, for a duration depending on the drug used. Unfortunately, no prophylaxis is 100% effective, and the appearance of fever during the travel or two to three months after return requires medical advice. In some circumstances, it is necessary to prescribe a stand-by emergency treatment, if no quick medical advice is possible. [less ▲]

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See detailPrevention de la mortalite secondaire apres anevrysme dissequant de type B
Quaniers, Janine ULiege; CREEMERS, Etienne ULiege; Djekic, J. et al

in Revue Médicale de Liège (2003), 58(6), 400-3

Only few dissecting aneurysms type A survive without surgery. Opposingly, the dissecting aneurysms type B are successfully managed by the medical treatment "anti-impulsion". A personal series is reported ... [more ▼]

Only few dissecting aneurysms type A survive without surgery. Opposingly, the dissecting aneurysms type B are successfully managed by the medical treatment "anti-impulsion". A personal series is reported, that evidence important cardiovascular mortality in the follow-up in chronic dissecting aneurysms type B; one part of this late mortality is linked to rupture of the thoracic descending aorta. Thus, the prevention of this late mortality depends on a strict follow-up of the characters and dimensions of descending aortic thoracic aorta. [less ▲]

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See detailLa tachycardie ventriculaire catecholergique du jeune enfant: un diagnostic souvent meconnu
Massin, M.; Leroy, P.; Misson, Jean-Paul ULiege et al

in Archives de Pédiatrie (2003), 10(6), 524-526

Catecholaminergic polymorphic ventricular tachycardia is important to be diagnosed as an underlying disease in children with syncope and normal heart, because of its poor prognosis. CASE REPORT: A 3-year ... [more ▼]

Catecholaminergic polymorphic ventricular tachycardia is important to be diagnosed as an underlying disease in children with syncope and normal heart, because of its poor prognosis. CASE REPORT: A 3-year-old boy was referred for stress and emotion induced syncope. Primary ventricular arrhythmia, consisting of salvos of bidirectional ventricular tachycardia, was reproducibly induced by physical exertion. The syncopal events and severe arrhythmia disappeared with beta-blocking therapy. CONCLUSION: Despite its rare occurrence, catecholaminergic polymorphic ventricular tachycardia is an important cause of stress and emotion induced syncope and sudden death in children. [less ▲]

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See detailThe electrophysiology of migraine
Ambrosini, A.; Schoenen, Jean ULiege

in Current Opinion in Neurology (2003), 16(3), 327-331

Purpose of review The pathophysiology of migraine is far from being understood. Electrophysiological methods are useful to investigate peripheral and central mechanisms underlying this disorder. The ... [more ▼]

Purpose of review The pathophysiology of migraine is far from being understood. Electrophysiological methods are useful to investigate peripheral and central mechanisms underlying this disorder. The purpose of this review is to highlight the results of electrophysiological studies published during the last year and to examine their added value to our previous knowledge. Recent findings Studies by visual and auditory evoked potentials and event-related responses suggested that lack of habituation is the principal interictal abnormality of sensory processing in migraineurs. Recently confirmed for somatosensory and laser-evoked cortical potentials and for brainstem responses, it is also responsible for the increased intensity dependence of auditory evoked potentials. This abnormality is possibly caused by a reduced cortical preactivation level due to hypofunctioning subcortico-cortical aminergic pathways. Although studies of cortical excitability by transcranial magnetic stimulation have yielded conflicting results, results obtained using habituation of pattern-reversal visual evoked potentials to explore cortical excitability changes induced by repetitive transcranial magnetic stimulation strongly favour the hypothesis that migraine is characterized by a decreased level of preactivation excitability. With regard to pain mechanisms in migraine, electrophysiological studies of trigeminal pathways using nociceptive blink and corneal reflexes have confirmed that sensitization of central trigeminal nociceptors occurs during the attack, and may even persist interictally. Summary Scientific publications over the last year confirmed that electrophysiological methods are particularly suited to unravelling some of the pathophysiological mechanisms of migraine. To improve their future contribution, they need to be better standardized and to be correlated with behavioural, metabolic and genetic studies. [less ▲]

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See detailDifferential gene expression in two potato lines differing in their resistance to Phytophthora infestans
Evers, Danièle; Ghislain, Marc; Hausman, Jean-François et al

in Journal of Plant Physiology (2003), 160(6), 709-712

Horizontal resistance to late blight in the potato is a primary objective of many breeding programs. Knowledge of the physiological and biochemical mechanisms underlying it, however, is scarce. The ... [more ▼]

Horizontal resistance to late blight in the potato is a primary objective of many breeding programs. Knowledge of the physiological and biochemical mechanisms underlying it, however, is scarce. The purpose of the present study was the identification of these physiological and biochemical factors in plant material obtained by crossing a late blight resistant Solanum phureja clone with a susceptible dihaploid of S. tuberosum subsp. tuberosum. The mRNA RT-PCR differential display method was used to compare the gene expression patterns of a resistant hybrid with that of a susceptible one. By sequence homology, we identified several genes with diverse functions, including genes known to be involved in resistance or stress responses and genes known to be involved in primary or secondary metabolism. [less ▲]

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See detailPreparation of BaZrO3 powders by a spray-drying process
Robertz, B.; Boschini, Frédéric ULiege; Rulmont, André ULiege et al

in Journal of Materials Research (2003), 18(6), 1325-1332

The potential use of barium zirconate for the manufacture of corrosion-resistant substrates emphasizes the need for a simple, inexpensive, and easily scalable process to produce high-quality powders with ... [more ▼]

The potential use of barium zirconate for the manufacture of corrosion-resistant substrates emphasizes the need for a simple, inexpensive, and easily scalable process to produce high-quality powders with well-controlled composition and properties. However, the classical solid-state preparation of barium zirconate leads to an inhomogeneous powder unsuitable for applications in highly corrosive environment. For this paper, the possibility to use the spray-drying technique for the preparation of BaZrO3 powders with a controlled size distribution and morphology was investigated. The influence of the nature and concentration of the precursor solution and the influence of the spray-drying step are discussed on the basis of x-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and dilatometric measurements. [less ▲]

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See detailPrevention de la necrose des membres inferieurs et de l'amputation par pontages femoro-tibiaux: indications, technique et resultats
Van Damme, Hendrik ULiege; Baguet, E.; Zhang, Lihong ULiege et al

in Revue Médicale de Liège (2003), 58(6), 415-28

Limb threatening ischemia is a challenge for the vascular surgeon. Recent progress in revascularization procedures allow to minimize the primary amputation rate in the management of chronic critical limb ... [more ▼]

Limb threatening ischemia is a challenge for the vascular surgeon. Recent progress in revascularization procedures allow to minimize the primary amputation rate in the management of chronic critical limb ischemia. The authors discuss the prevalence and causes of chronic critical limb ischemia, with a special interest for diabetic arteriopathy. The technique of crural and pedal vessel revascularization is described, as well as the innovative tourniquet technique for distal bypass surgery. A review of published series of infrapopliteal bypass surgery is made. The experience of the authors during last decade with crural and pedal bypass surgery is analyzed. [less ▲]

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See detailLa prise en charge cardiaque preoperatoire du patient vasculaire
Van Damme, Hendrik ULiege; Larbuisson, Robert ULiege; Limet, Raymond ULiege

in Revue Médicale de Liège (2003), 58(6), 409-14

Peripheral vascular surgery (carotid, infrainguinal or aortoiliacal) is characterised by an increased cardiac risk with an infarction rate of 1 to 4%. Sixty percent of the vascular patients present a ... [more ▼]

Peripheral vascular surgery (carotid, infrainguinal or aortoiliacal) is characterised by an increased cardiac risk with an infarction rate of 1 to 4%. Sixty percent of the vascular patients present a concomitant coronary artery disease, often infraclinically. Preoperative cardiac risk stratification aims at reducing cardiac related morbidity and mortality. A clinical risk profile (patient's past history) and non-invasive cardiac tests allow subdividing the vascular patients into three categories: high risk, intermediate risk, and low risk. High-risk patients (unstable angina, recent infarction, overt congestive heart failure and critical aortic valve stenosis) require immediate intensive management of their underlying cardiac disease. This means delay or annulation of the planned vascular operation. For intermediate risk patients, a clinical cardiac risk index based on patient's past history (stable angina, previous infarction or episode of congestive heart failure, age of 70 years or more and diabetes) offers a rough orientation. These clinical markers lack specificity, since they are found in almost all vascular patients. The adjunction of a non-invasive cardiac testing allows to optimise the cardiac risk evaluation. Stress echocardiography with dobutamine has become a very popular test, with a negative predicting value exceeding 90%, but with a lack of specificity (many vascular patients have an uneventful postoperative outcome, despite a positive dobutamine test). These inconsistent results of cardiac risk evaluation render their routine use questionable. Nowadays, the management of patients requiring vascular surgery is based on the concept that every vascular patient should be considered as suffering from coronary artery disease. A certain degree of myocardial protection should be offered to every vascular surgery candidate. A preoperative treatment with betablockers provides myocardial protection against the operative stress and lowers myocardial oxygen requirement. There are arguments to continue or start aspirin treatment in the preoperative period, in order to lower the risk of sudden coronary thrombosis. [less ▲]

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See detailEvaluation, severity and prognostic significance of silent myocardial ischaemia in vascular patients
Sprynger, Muriel ULiege

in Acta Chirurgica Belgica (2003), 103(3), 255-261

This paper intends to make an update of recent publications and guidelines for evaluation in coronary symptom-free patients undergoing vascular surgery. It emphasizes the role of preoperative clinical ... [more ▼]

This paper intends to make an update of recent publications and guidelines for evaluation in coronary symptom-free patients undergoing vascular surgery. It emphasizes the role of preoperative clinical evaluation that should identify the most appropriate testing, and treatment strategies to optimize care of the patient and avoid unnecessary testing in this era of cost containment. Selective preoperative coronary artery disease screening and revascularization achieve excellent perioperative and late results after high-risk vascular surgery. Supplemental preoperative evaluation is discussed (exercise ECG, stress echocardiography and stress tomoscintigraphy). Asymptomatic patients with good functional capacity can undergo intermediate-risk surgery without further non-invasive testing. Conversely, further noninvasive testing is often considered for patients with poor functional capacity or moderate functional capacity but higher-risk surgery especially for patients with 2 or more intermediate risk predictors. Additional testing may be considered on an individual basis for patients without clinical markers but with poor functional capacity prior to vascular surgery, particularly those with several minor clinical risk predictors. Because of a higher prevalence of silent myocardial ischaemia in diabetes mellitus, these patients require specific care. Until further data are available, indications for myocardial revascularization in the perioperative setting are similar to those in the ACC/AHA guidelines for use of myocardial revascularization in general. General practioners, cardiologists, angiologists, vascular surgeons and anaesthesiologists should collaborate and aim to slow down the progression of atherosclerosis by giving their patients an optimum secondary cardiovascular prevention. [less ▲]

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See detailLong-term memory effects on verbal short-term memory : a replication study
Majerus, Steve ULiege; Van der Linden, Martial ULiege

in British Journal of Developmental Psychology (2003), 21(Part 2), 303-310

The influence of lexico-semantic language representations stored in long-term memory (LTM) on short-term memory (STM) performance has been studied extensively in adults. However, there are relatively few ... [more ▼]

The influence of lexico-semantic language representations stored in long-term memory (LTM) on short-term memory (STM) performance has been studied extensively in adults. However, there are relatively few data on lexico-semantic LTM effects on STM in children. On the other hand, the influence of phonological LTM effects on STM has been studied more extensively in children than in adults. In this study, we explored whether these different LTM effects on verbal STM could be replicated in both adults and children by administering immediate serial recall tasks (ISR) for high- and low-frequency words, for high- and low-imageability words, for words and non-words, and for high and low phonotactic frequency non-words to 6-, 8-, and 10-year-old children, to adolescents and to adults. Significant word frequency, lexicality and phonotactic frequency effects were observed in all age groups, as well as a word imageability effect which was, however, weaker than the other three effects. Our data suggest that LTM effects on STM are equivalent in both children and adults. [less ▲]

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See detailTechnology Development for In-Flight Oxygen Collection TSTO's
Hendrick, Patrick; Verstraete, D.; Bizzarri, Didier et al

in European Journal of Mechanical and Environmental Engineering (2003), 48 n°2

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See detailCommunication, positionnement et GPS
Debouche, Charles ULiege

in Journal des Ingénieurs (Le) (2003), 87

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See detailDeterminants of left ventricular preload-adjusted maximal power
Segers, Patrick; Tchano-Sato, Vincent; Leather, H. Alex et al

in American Journal of Physiology - Heart and Circulatory Physiology (2003), 284(6), 2295-2301

Maximal left ventricular (LV) hydraulic power output (PWRmax), corrected for preload as PWRmax/(V-ed)(beta) (where V-ed is the end-diastolic volume and beta is a constant coefficient), is an index of LV ... [more ▼]

Maximal left ventricular (LV) hydraulic power output (PWRmax), corrected for preload as PWRmax/(V-ed)(beta) (where V-ed is the end-diastolic volume and beta is a constant coefficient), is an index of LV contractility. Whereas preload-adjusted maximal power (PAMP) is usually calculated with beta = 2, there is uncertainty about the optimal value of beta (beta = 1 for the normal LV and 2 for the dilated LV). The aim of this work is to study the determining factors of beta. The data set consisted of 245 recordings (steady state and vena cava occlusion) in 10 animals in an ischemic heart pig model. The occlusion data yielded the slope (E-es; 2.01 +/- 0.77 mmHg/ml, range 0.71-4.16 mmHg/ml) and intercept (V-0; -11.9 +/- 22.6 ml; range -76 to 39 ml) of the end-systolic pressure-volume relation, and the optimal beta-factor (assessed by fitting an exponential curve through the V-ed-PWRmax relation) was 1.94 +/- 0.88 (range 0.29-4.73). The relation of beta with V-ed was weak [beta = 0.60 + 0.02(V-ed); r(2) = 0.20]. In contrast, we found an excellent exponential relation between V-0 and beta [beta = 2.16e(0.0189(V0)), r(2) = 0.70]. PAMP, calculated from the steady-state data, was 0.64 +/- 0.40 mW/ml(2) (range 0.14-2.83 mW/ml(2)) with a poor correlation with E-es (r = 0.30, P < 0.001). An alternative formulation of PAMP as PWRmax/(V-ed - V-0)(2), incorporating V-0, yielded 0.47 +/- 0.26 mW/ml(2) (range 0.09-1.42 mW/ml(2)) and was highly correlated with E-es (r = 0.89, P < 0.001). In conclusion, correct preload adjustment of maximal LV power requires incorporation of V-0 and thus of data measured under altered loading conditions. [less ▲]

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See detailBehavior of bulk melt-textured YBCO single domains subjected to crossed magnetic fields
Vanderbemden, Philippe ULiege; Dorbolo, Stéphane ULiege; Hari-Babu, N. et al

in IEEE Transactions on Applied Superconductivity (2003), 13(2, Part 3), 3746-3749

We have experimentally investigated the crossed magnetic field effects on bulk melt-processed YBCO single domains. The samples were first permanently magnetized along their c-axis and then subjected to ... [more ▼]

We have experimentally investigated the crossed magnetic field effects on bulk melt-processed YBCO single domains. The samples were first permanently magnetized along their c-axis and then subjected to several cycles of a transverse magnetic field parallel to the ab planes. The magnetic properties along the c and ab directions were simultaneously measured using a couple of orthogonal pick-up coils as well as a Hall probe placed against the sample surface. The effects of both sweep amplitude and polarity were investigated. Field sweeps of alternate polarities are shown to affect the decay of the c-axis magnetization much more strongly than field sweeps of unique polarity do. However, the c-axis magnetization does not show any saturation even after a large number of field sweeps. Next, a micro-Hall probe scanning system was used to measure the distribution of magnetic induction over the top surface of the single domain subjected to the same combination of magnetic fields. The results are shown to be consistent with those determined with the sensing coils and bring out the role played by geometric effects. (C) 2003 IEEE. [less ▲]

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See detailAlteration of left ventriculo-arterial coupling and mechanical efficiency during acute myocardial ischemia
Kolh, Philippe ULiege; Lambermont, Bernard ULiege; Ghuysen, Alexandre ULiege et al

in International Angiology (2003), 22(2), 148-158

AIM: Myocardial revascularisation being frequently performed during acute myocardial ischemia, in a hostile hemodynamic environment, we evaluated left ventriculo-arterial (VA) coupling, left ventricular ... [more ▼]

AIM: Myocardial revascularisation being frequently performed during acute myocardial ischemia, in a hostile hemodynamic environment, we evaluated left ventriculo-arterial (VA) coupling, left ventricular (LV) mechanical efficiency, and the mechanical properties of the systemic vasculature during acute myocardial ischemia. METHODS: In 6 pigs, vascular properties [characteristic impedance (R(1)), peripheral resistance (R(2)), compliance (C), inductance (L), arterial elastance (E(a))] were estimated with a windkessel model. LV function was assessed by the slope (E(es)) of end-systolic pressure-volume relationship (ESPVR), and stroke work (SW) - end-diastolic volume (EDV) relation. Pressure-volume area (PVA) was referred to as myocardial oxygen consumption. VA coupling was defined as E(es)/E(a), and mechanical efficiency as SW/PVA. After baseline recordings, the left anterior descending coronary artery was ligated and hemodynamic measures obtained every 30 minutes for 3 hours. Data are expressed as mean (SEM). RESULTS: Coronary occlusion induced an ESPVR rightward shift, and decreased E(es) from 3.67 (0.33) to 1.92 (0.20) mmHg/ml and the slope of the SW - EDV relationship from 72.3 (3.4) to 40.4 (4.5) mmHg (p<0.001), while E(a) increased from 3.33 (0.56) to 4.65 (0.29) mmHg/ml (p<0.005). This was responsible for a dramatic alteration of VA coupling from 1.22 (0.11) to 0.44 (0.07), (p<0.001). While R2 increased from 1.72 (0.30) to 2.38 (0.16) mmHg x s x ml(-1) (p<0.05) and C decreased from 0.78 (0.16) to 0.46 (0.08) ml/mmHg (p<0.05), R(1) and L were unchanged. Coronary occlusion decreased SW from 4056 (223) to 2580 (122) mmHg.ml (p<0.001), while PVA and SW/PVA decreased from 5575 (514) to 4813 (317) mmHg x ml (NS), and from 0.76 (0.04) to 0.57 (0.03) (p<0.001), respectively. CONCLUSION: Acute myocardial ischemia severely altered left ventriculo-arterial coupling and LV mechanical efficiency. Impaired left VA coupling was due to a combination of augmented arterial elastance, secondary to early vasoconstriction later associated with decreased arterial compliance, and decreased LV contractility. [less ▲]

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See detailDesorption behaviour of regular adsorbed polyampholytic layers
Mahltig, Boris; Gohy, Jean-François; Jérôme, Robert ULiege et al

in Journal of Polymer Research (2003), 10(2), 69-77

The desorption behaviour of the diblock polyampholyte PMAA-b-PDMAEMA, poly(methacrylic acid)-block-poly ((dimethylamino)ethyl methacrylate), preadsorbed on silicon substrates was investigated under the ... [more ▼]

The desorption behaviour of the diblock polyampholyte PMAA-b-PDMAEMA, poly(methacrylic acid)-block-poly ((dimethylamino)ethyl methacrylate), preadsorbed on silicon substrates was investigated under the influence of several desorption agents. The investigated polyampholyte is known to adsorb in regular structures directly from aqueous solutions onto silicon substrates. While the adsorption process is mainly determined by electrostatic interactions, two kinds of desorption mechanism should be assumed. The first mechanism is based on changed electrostatic conditions caused for instance by a strong change in pH of the aqueous solution. The other mechanism is observed after treatment with hydrophobic organic solvent, which leads to the desorption of hydrophobic adsorbed polyampholyte chains, while the electrostatically attached ones will not be influenced. To complete the desorption experiments with organic solvents also adsorption experiments from analogous polyampholytic solutions in the same organic solvents were performed. The amount of polymer at the substrate surface after adsorption or desorption experiments was determined using ellipsometry. Atomic force microscopy (AFM) was used to investigate the surface topography of dried samples after the desorption process. [less ▲]

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See detailFaire face à une suspicion de parvovirose canine
Thiry, Etienne ULiege

in Point Vétérinaire (2003), 34(236, Suppl. S), 39633

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See detailPre- and post-treatment with pirlindole and dehydropirlindole protects cultured brain cells against nitric oxide-induced death.
Boland, André ULiege; Gérardy, Jean; Mossay, Danielle ULiege et al

in European Journal of Pharmacology (2003), 466

We have previously shown that pirlindole and dehydropirlindole, two monoamine oxidase type-A inhibitors, protect cultured brain cells against iron-induced toxicity through a mechanism unrelated to ... [more ▼]

We have previously shown that pirlindole and dehydropirlindole, two monoamine oxidase type-A inhibitors, protect cultured brain cells against iron-induced toxicity through a mechanism unrelated to monoamine oxidase type-A inhibition. The current study was performed to test whether the protective effect of pirlindole and dehydropirlindole could be extended to a nitric oxide (NO)-induced insult. A comparison with other monoamine oxidase inhibitors (brofaromine, moclobemide and deprenyl) and with trolox was made. In a first series of experiments, rat hippocampal or cortical cultured cells were exposed to a drug for 3 h, then 5 muM sodium nitroprusside, a NO donor, was added and the incubation was continued for 16 h. Cell survival assessment showed that pirlindole, dehydropirlindole and trolox significantly protected cultures against NO-induced toxicity in a concentration-dependent manner with respective EC50's of 7, 3 and 17 muM. Similarly, pirlindole, dehydropirlindole or trolox, at a concentration of 50 muM, significantly decreased both intracellular peroxide production and lipoperoxidation. Other drugs were ineffective. In a post-hoc treatment protocol (3- or 6-h pre-incubation in the presence of sodium nitroprusside, then addition of one of the above mentioned compounds), only pirlindole and dehydropirlindole significantly improved cell survival in a concentration-dependent manner with respective EC50'S of 9 and 4 muM. The maximal protection in terms of cell survival was 90% and 78% after 3 and 6 h, respectively. They also reduced the production of both lipoperoxides and endoperoxides. Our results show that pirlindole and dehydropirlindole protect neurons against NO-induced toxicity at pharmacologically relevant concentrations. Moreover, their protective effect is still apparent when they are applied after the start of the insult. Therefore, our preclinical study suggests a new strategy that may be efficient to reduce NO-induced damage in the central nervous system. (C) 2003 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailPhase shift effective range expansion from supersymmetric quantum mechanics
Samsonov, B. F.; Stancu, Floarea ULiege

in Physical Review C (2003), 67(5), 054005

Supersymmetric or Darboux transformations are used to construct local phase equivalent deep and shallow potentials for lnot equal0 partial waves. We associate the value of the orbital angular momentum ... [more ▼]

Supersymmetric or Darboux transformations are used to construct local phase equivalent deep and shallow potentials for lnot equal0 partial waves. We associate the value of the orbital angular momentum with the asymptotic form of the potential at infinity, which allows us to introduce adequate long-distance transformations. The approach is shown to be effective in getting the correct phase shift effective range expansion. Applications are considered for the P-1(1) and D-1(2) partial waves of the neutron-proton scattering. [less ▲]

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See detailOsteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells
Barnes, G. L.; Javed, A.; Waller, S. M. et al

in Cancer Research (2003), 63(10), 2631-2637

Human breast cancers are known to preferentially metastasize to skeletal sites, however, the mechanisms that mediate the skeletal preference (orthotropism) of specific types of cancers remains poorly ... [more ▼]

Human breast cancers are known to preferentially metastasize to skeletal sites, however, the mechanisms that mediate the skeletal preference (orthotropism) of specific types of cancers remains poorly understood. There is a significant clinical correlation between the expression of bone sialoprotein (BSP) and skeletal metastasis of breast cancers. Our laboratory, as well as others, have proposed the concept that skeletal selective metastasis and associated disease may be attributable to a mimicry of skeletal cellular phenotypes by metastasizing cancer cells. We hypothesize that breast cancer cell expression of phenotypic properties of skeletal cell types, including BSP as one component of that phenotype, is the result of ectopic expression or activity of one or more central transcriptional regulators of bone cell gene expression. To test this hypothesis, we examined the molecular mechanisms that regulate bsp expression in human breast cancer cell lines with previously characterized metastatic potentials. Our results demonstrate that the majority of the distal bsp promoter sequences act to repress BSP expression in cancer cells and that most of the promoter activity resides in the proximal -110 by of the bsp promoter. In this region, we identified a putative Runx binding element providing a basis for a mechanism for skeletal gene activation. Our results demonstrate that Runx2 is ectopically expressed in breast cancer cells and that one isoform of Runx2 can activate bsp expression in these cells. In addition, we observe that bsp expression is additionally regulated by the homeodomain factor Msx2, another regulator of osteoblast-associated genes. Thus, this is the first report of osteoblast-related transcription factors being expressed in human breast cancer cells and provides a component of a mechanism that may explain the osteoblastic phenotype of human breast cancer cells that preferentially metastasize to bone. [less ▲]

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See detailLa vie politique belge est un ‘jardin extraordinaire’
Verjans, Pierre ULiege

in Courrier international (2003), (n°654), 12-13

Du côté flamand, on vote conservateur, et le Vlaams Blok vampirise le débat politique ; du côté wallon, on penche à gauche, et l'extrême droite semble marginalisée. Le scrutin du 18 mai ne changera pas ... [more ▼]

Du côté flamand, on vote conservateur, et le Vlaams Blok vampirise le débat politique ; du côté wallon, on penche à gauche, et l'extrême droite semble marginalisée. Le scrutin du 18 mai ne changera pas cette donne. [less ▲]

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See detailIntermediate basal cells of the prostate: In vitro and in vivo characterization
Garraway, Levi; Lin, Douglas; Signoretti, Sabina et al

in Prostate (2003), 55(3), 206-218

BACKGROUND. Progenitor cells within the prostate basal layer may play important roles in differentiation and carcinogenesis; however, prostate stem cell populations remain uncharacterized. METHODS ... [more ▼]

BACKGROUND. Progenitor cells within the prostate basal layer may play important roles in differentiation and carcinogenesis; however, prostate stem cell populations remain uncharacterized. METHODS. Immunohistochemical and immunoblot analyses were used to characterize prostate epithelial cells (PrEC), a commercially available prostate basal cell isolate. RESULTS. Proliferating PrECs exhibited immunophenotypic characteristics most consistent with basal cells, but during senescence PrECs up-regulated androgen receptor (AR) mRNA, p27, and low-molecular-weight cytokeratin (LMWCK) expression, suggestive of partial differentiation. PrECs also stained strongly for involucrin, which marked a subset of intermediate prostate basal cells in vivo. Basal hyperplasia consisting of involucrin-positive cells was prevalent in prostate tissue from androgen-ablated patients, and formed epithelial clusters flanked by involucrin-negative basal and luminal monolayers. Cultivation of PrECs on matrigel together with androgen-treated stromal conditioned media resulted in dense aggregates, with a peripheral rim of basal-like cells expressing p63 and basal cytokeratins. CONCLUSIONS. PrEC represents an epithelial population whose basal characteristics are modified in response to matrigel, stromal factors, and senescence, consistent with a transient amplifying population. These cells may derive from a previously unrecognized, involucrin-positive subset present in vivo. (C) 2003 Wiley-Liss, Inc. [less ▲]

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See detailRadiative lifetime measurements in ErII by time-resolved laser spectroscopy
Xu, H. L.; Jiang, Z. K.; Zhang, Z. G. et al

in Journal of Physics B-Atomic Molecular and Optical Physics (2003), 36(9), 1771-1781

Radiative lifetimes of 30 excited states of Er 11, ranging in energy from 25 592 to 36 148 cm(-1), have been measured by means of a time-resolved laser-induced fluorescence (LIF) technique. Free, singly ... [more ▼]

Radiative lifetimes of 30 excited states of Er 11, ranging in energy from 25 592 to 36 148 cm(-1), have been measured by means of a time-resolved laser-induced fluorescence (LIF) technique. Free, singly ionized erbium ions were produced in a laser-induced plasma. A tunable laser, with 1 ns duration pulse, was employed to selectively excite the Er+ ions. The lifetime values were evaluated from the transient LIF signals recorded by a fast detection system. A comparison of the new results with previously published values is given. HFR calculations, including core-polarization contributions, are found to be in reasonable agreement with experiment, but the present calculations clearly indicate that further laboratory and theoretical work is urgently needed, particularly for the low lying odd configurations of this complex ion. [less ▲]

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See detailIdentification of a continuous structure with a geometrical non-linearity. Part II: Proper orthogonal decomposition
Lenaerts, V.; Kerschen, Gaëtan ULiege; Golinval, Jean-Claude ULiege

in Journal of Sound & Vibration (2003), 262(4), 907-919

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex ... [more ▼]

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex systems. The aim of this paper and a companion paper is to study, by means of two methods, a continuous non-linear system consisting of an experimental cantilever beam with a geometrical non-linearity. In the companion paper (Part I) [1] the ability of the conditioned reverse path method, which is a frequency domain technique, to identify the behaviour of this structure-is assessed. This paper (Part II) is devoted to the application of proper orthogonal decomposition, which is an updating technique, to the test example. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailIdentification of a continuous structure with a geometrical non-linearity. Part I: Conditioned reverse path method
Kerschen, Gaëtan ULiege; Lenaerts, V.; Golinval, Jean-Claude ULiege

in Journal of Sound & Vibration (2003), 262(4), 889-906

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex ... [more ▼]

Particular effort has been spent in the field of identification of multi-degree-of-freedom non-linear systems. The newly developed methods permit the structural analyst to consider increasingly complex systems. The aim of this paper and a companion paper is to study, by means of two methods, a continuous non-linear system consisting of an experimental cantilever beam with a geometrical non-linearity. In this paper (Part I), the ability of the conditioned reverse path method, which is a frequency domain technique, to identify the behaviour of this structure is assessed. The companion paper (Part II) is devoted to the application of proper orthogonal decomposition, which is an updating technique, to the test example. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailTime correlations and 1/f behavior in backscattering radar reflectivity measurements from cirrus cloud ice fluctuations
Ivanova, K.; Ackerman, T. P.; Clothiaux, E. E. et al

in Journal of Geophysical Research. Atmospheres (2003), 108(D9),

[1] The state of the atmosphere is governed by the classical laws of fluid motion and exhibits correlations in various spatial and temporal scales. These correlations are crucial to understand the short ... [more ▼]

[1] The state of the atmosphere is governed by the classical laws of fluid motion and exhibits correlations in various spatial and temporal scales. These correlations are crucial to understand the short- and long-term trends in climate. Cirrus clouds are important ingredients of the atmospheric boundary layer. To improve future parameterization of cirrus clouds in climate models, it is important to understand the cloud properties and how they change within the cloud. We study correlations in the fluctuations of radar signals obtained at isodepths of winter and fall cirrus clouds. In particular, we focus on three quantities: (1) the backscattering cross-section, (2) the Doppler velocity, and (3) the Doppler spectral width. They correspond to the physical coefficients used in Navier Stokes equations to describe flows, i.e., bulk modulus, viscosity, and thermal conductivity. In all cases we find that power law time correlations exist with a crossover between regimes at about 3 to 5 min. We also find that different type of correlations, including 1/f behavior, characterize the top and the bottom layers and the bulk of the clouds. The underlying mechanisms for such correlations are suggested to originate in ice nucleation and crystal growth processes. [less ▲]

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See detailLes nouveaux antifongiques
Hayette, Marie-Pierre ULiege

in Noso-Bulles, bulletin d'information du comité d'hygiène hospitalière et du GGA du CHU de liège (2003)

L'article est une mise au point sur les antifongiques avec leurs indications, toxicité, posologie et critères de remboursement.

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See detailA spherical metric for the field-oriented analysis of complex urban open spaces
Teller, Jacques ULiege

in Environment & Planning B : Planning & Design (2003), 30(3), 339-356

The author deals with the analysis of urban open spaces, once conceived as part and parcel of our urban heritage. He introduces a mathematical modelling technique that is capable of mapping the variation ... [more ▼]

The author deals with the analysis of urban open spaces, once conceived as part and parcel of our urban heritage. He introduces a mathematical modelling technique that is capable of mapping the variation of the sky visible from points distributed throughout space. The resulting maps overcome the limits of orthographic (plan, section, and elevation) and perspective methods of analysis by considering the dynamic qualities of the Gibsonian 'visual world' that takes account not only of bifocal vision but also of the relatively free movement of the head and shoulders, that is, vision as part of the human ecology. The maps show how-a person might experience those volumes of a void that define a space, not from a fixed point but from moving about inside the entire urban open space. [less ▲]

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See detailXMM-Newton high-resolution X-ray spectroscopy of the Wolf-Rayet object WR 25 in the Carina OB1 association
Raassen, A. J. J.; van der Hucht, K. A.; Mewe, R. et al

in Astronomy and Astrophysics (2003), 402(2), 653-666

We report the analysis of the first high-resolution X-ray spectra of the Wolf-Rayet (WR) object WR25 (HD 93162, WN6ha+O4f) obtained with the reflection Grating spectrometers (RGS) and the European photon ... [more ▼]

We report the analysis of the first high-resolution X-ray spectra of the Wolf-Rayet (WR) object WR25 (HD 93162, WN6ha+O4f) obtained with the reflection Grating spectrometers (RGS) and the European photon imaging cameras (EPIC-MOS and PN) CCD spectrometers on board the XMM-Newton satellite. The spectrum exhibits bright emission lines of the H- and He-like ions of Ne, Mg, Si and S, as well as Fe XVIII to Fe XX and Fe XXV lines. Line fluxes have been measured. The RGS and e pi c spectra have been simultaneously fitted to obtain self-consistent temperatures, emission measures, and elemental abundances. Strong absorption by the dense WR stellar wind and the interstellar medium (ISM) is observed equivalent to N-H = 7x10(21) cm(-2). Multi-temperature (DEM) fitting yields two dominant components around temperatures of 7.0 and 32 MK, respectively. The XMM intrinsic (i.e. unabsorbed, corrected for the stellar wind absorption and the absorption of ISM) X-ray luminosity of WR25 is L-x(0.5-10 keV) = 1.3x10(34) erg s(-1), and L-x(0.5-10 keV) = 0.85 x 10(34) erg s(-1), (when correcting for the ISM only) assuming d = 3.24 kpc. The obtained chemical abundances are subsolar, except for S. This may be real, but could equally well be due to a weak coupling to the continuum, which is strongly influenced by the absorption column density and the subtracted background. The expected high N-abundance, as observed in the optical wavelength region, could not be confirmed due to the strong wind absorption, blocking out its spectral signature. The presence of the Fe XXV emission-line complex at similar to6.7 keV is argued as being indicative for colliding winds inside a WR+O binary system. [less ▲]

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See detailInteret et limites du depistage de masse du cancer du sein par mammographie seule (mammotest)
Lifrange, Eric ULiege; Bleret, Valerie ULiege; Desreux, Joëlle ULiege et al

in Revue Médicale de Liège (2003), 58(5), 331-7

The literature on screening mammography provides ample opportunity for doubt (the sceptics) and dogma (the screening zealots), and can be interpreted to prove both benefit and harm. The benefit of ... [more ▼]

The literature on screening mammography provides ample opportunity for doubt (the sceptics) and dogma (the screening zealots), and can be interpreted to prove both benefit and harm. The benefit of mammography screening, if any, is modest and the balance between beneficial (potentially, a 20% relative reduction in breast cancer mortality with no significant benefit on all-cause mortality) and harmful (physical and psychological morbidity related to the 15-40% missed cancers and the 80-90% false-positive diagnoses) effects is still delicate. The mammogram alone is a modest weapon. Concurrent clinical breast examination is mandatory. Women that are concerned about breast cancer should be fully informed of the potential benefits and risks of screening mammography. These women should benefit from mammography with concurrent clinical breast examination, and possible whole-breast ultrasound in heterogeneously dense and extremely dense breast patterns. [less ▲]

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See detailInfluence of cisplatin-use, age, performance status and duration of chemotherapy on symptom control in advanced non-small cell lung cancer: detailed symptom analysis of a randomised study comparing cisplatin-vindesine to gemcitabine
Vansteenkiste, J.; Vandebroek, J.; Nackaerts, K. et al

in Lung Cancer (2003), 40(2), 191-199

Background: We previously reported that treatment of patients with symptomatic advanced non-small cell lung cancer with single agent Gemcitabine (GEM) resulted in a superior clinical-benefit response rate ... [more ▼]

Background: We previously reported that treatment of patients with symptomatic advanced non-small cell lung cancer with single agent Gemcitabine (GEM) resulted in a superior clinical-benefit response rate (RR) compared to cisplatin-based combination chemotherapy. We now report the detailed individual symptom control analysis, and the influence of cisplatin-use, age, performance status (PS) and duration of treatment. Patients and methods: Patients received either GEM (1000 mg/m(2), days 1, 8 and 15) or cisplatin (100 mg/m(2), day 1) plus Vindesine (3 mg/m(2), days 1 and 15) (PV), both every 4 weeks. Scores of 9 symptoms were listed weekly by the patient on visual analogue scales. Improvement of a symptom was defined as 2 consecutive cycles of improvement over baseline. Results: Baseline symptoms in the 169 patients were well balanced between the 2 arms (84 GEM, 85 PV). Both patients with objective response and disease stabilisation had clearly better symptom control than those with disease progression. Symptom control in both arms was similar for 'disease-specific' symptoms such as cough, dyspnea, pain or haemoptysis. Compared to PV, a significantly larger number of GEM-patients had better scores for 'constitutional' items such as anorexia (P = 0.007), ability to carry on with daily activities (P = 0.04) and overall impression of quality-of-life (P = 0.008). Symptom control was very similar in younger (< 65 years) versus older (> 65 years) patients, and only slightly better in those with a Karnofsky PS greater than or equal to 80% compared to those < 80%. Most of the symptom improvement occurred in the first 3 cycles, with some further symptom improvement in the following cycles in the GEM-arm only. Conclusions: Both GEM and PV yield a symptom control rate much higher than expected by the objective tumour RR. GEM is equally effective in controlling 'disease-specific' symptoms, but superior in controlling 'constitutional' symptoms. Most of the symptom control was achieved during the first 3 cycles of treatment, with some further improvement thereafter in the GEM-arm only. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailBioresorbable and bioactive composite materials based on polylactide foams filled with and coated by Bioglass (R) particles for tissue engineering applications
Boccaccini, Aldo R.; Notingher, I.; Maquet, Véronique et al

in Journal of Materials Science: Materials in Medicine (2003), 14(5), 443-450

Poly(DL-lactide) (PDLLA) foams and bioactive glass (Bioglass®) particles were used to form bioresorbable and bioactive composite scaffolds for applications in bone tissue engineering. A thermally induced ... [more ▼]

Poly(DL-lactide) (PDLLA) foams and bioactive glass (Bioglass®) particles were used to form bioresorbable and bioactive composite scaffolds for applications in bone tissue engineering. A thermally induced phase separation process was applied to prepare highly porous PDLLA foams filled with 10wt% Bioglass® particles. Stable and homogeneous layers of Bioglass® particles on the surface of the PDLLA/Bioglass® composite foams as well as infiltration of Bioglass® particles throughout the porous network were achieved using a slurry-dipping technique. The quality of the bioactive glass coatings was reproducible in terms of thickness and microstructure. In vitro studies in simulated body fluid (SBF) were performed to study the formation of hydroxyapatite (HA) on the surface of the PDLLA/Bioglass® composites, as an indication of the bioactivity of the materials. Formation of the HA layer after immersion in SBF was confirmed by X-ray diffraction and Raman spectroscopy measurements. The rate of HA formation in Bioglass®-coated samples was higher than that observed in non-coated samples. SEM analysis showed that the HA layer thickness rapidly increased with increasing time in SBF in the Bioglass®-coated samples. The high bioactivity of the developed composites suggests that the materials are attractive for use as bioactive, resorbable scaffolds in bone tissue engineering. [less ▲]

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See detailTransactivation of vimentin by beta-catenin in human breast cancer cells
Gilles, Christine ULiege; Polette, M.; Mestdagt, Mélanie ULiege et al

in Cancer Research (2003), 63(10), 2658-2664

The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive ... [more ▼]

The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive/migratory properties of epithelial cells. Because beta-catenin can act as a coactivator of transcription through its binding to the T-cell factor (TCF)/lymphoid enhancer factor 1 transcription factor family, we have explored the possibility that beta-catenin/TCF could directly transactivate vimentin. We first compared vimentin expression in relation with the localization of beta-catenin in eight breast cancer cell lines displaying various degrees of invasiveness and in a model of cell migration using human mammary MCF10A cells. We could thus show a cytoplasmic and/or nuclear distribution of beta-catenin in invasive/migratory cells expressing vimentin, but not in noninvasive/stationary vimentin-negative cell lines. In addition, the human vimentin promoter was found to be up-regulated by beta-catenin and TCF-4 cotransfection. Varying with the cellular background, a diminution of this up-regulation was observed when the putative beta-catenin/TCF binding site of the vimentin promoter was mutated. Our results therefore demonstrate that the vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion. [less ▲]

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See detailPrevention secondaire des accidents ischemiques coronariens par la chirurgie de pontage aorto-coronaire
Radermecker, Marc ULiege; Limet, Raymond ULiege

in Revue Médicale de Liège (2003), 58(5), 283-6

Coronary artery bypass surgery has shown its superiority on other therapeutic options in specific but frequent indications such as left main lesions, multivessel disease with alteration of ventricular ... [more ▼]

Coronary artery bypass surgery has shown its superiority on other therapeutic options in specific but frequent indications such as left main lesions, multivessel disease with alteration of ventricular function, proximal lesions of the LAD, and multivessel diseases in diabetic patients. After reviewing the epidemiology of coronary artherosclerosis, we emphasize the efficacy of the surgical treatment compared to medical or interventional therapy for preventing ischemic events. The results derived from randomized studies may be even further improved by the implementation of beating heart surgery and the systematic use of multiple arterial conduits. [less ▲]

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See detailArrest of apoptosis in auditory neurons: Implications for sensorineural preservation in cochlear implantation
Scarpidis, U.; Madnani, D.; Shoemaker, C. et al

in Otology & Neurotology (2003), 24(3), 409-417

Hypothesis: The JNK/c-Jun cell death pathway is a major pathway responsible for the loss of oxidative stress-damaged auditory neurons. Background: Implantation of patients with residual hearing ... [more ▼]

Hypothesis: The JNK/c-Jun cell death pathway is a major pathway responsible for the loss of oxidative stress-damaged auditory neurons. Background: Implantation of patients with residual hearing accentuates the need to preserve functioning sensorineural elements. Although some auditory function may survive electrode insertion, the probability of initiating an ongoing loss of auditory neurons and hair cells is unknown. Cochlear implantation can potentially generate oxidative stress, which can initiate the cell death of both auditory neurons and hair cells. Methods: Dissociated cell cultures of P4 rat auditory neurons identified the apoptotic pathway initiated by oxidative stress insults (e.g., loss of trophic factor support) and characterized this pathway by arresting translation of pathway-specific mRNA with antisense oligonucleotide treatment and with the use of pathway specific inhibitors. The presence or absence of apoptosis-specific protein and changes in the level of neuronal survival measured the efficacy of these interventional strategies. Results: These in vitro studies identified the JNK/c-Jun cascade as a major initiator of apoptosis of auditory neurons in response to oxidative stress. Neurons pretreated with c-jun antisense oligonucleotide and exposed to high levels of oxidative stress were rescued from apoptosis, whereas neurons in treatment control cultures died. Treatment of oxidative-stressed cultures with either curcumin, a MAPKKK pathway inhibitor, or PD-098059, a MEK1 inhibitor, blocked loss of neurons via the JNK/c-Jun apoptotic pathway. Conclusion: Blocking the JNK/c-Jun cell death pathway is a feasible approach to treating oxidative stress-induced apoptosis within the cochlea and may have application as an otoprotective strategy during cochlear implantation. [less ▲]

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See detailBreast cancer and serum organochlorine residues
Charlier, Corinne ULiege; Albert, Adelin ULiege; Herman, Philippe ULiege et al

in Occupational and Environmental Medicine (2003), 60(5), 348-351

Background: Controversy still exists about the breast carcinogenic properties in humans of environmental xenoestrogens (organochlorines), justifying new investigations. Aims: To compare the blood levels ... [more ▼]

Background: Controversy still exists about the breast carcinogenic properties in humans of environmental xenoestrogens (organochlorines), justifying new investigations. Aims: To compare the blood levels of total dichlorodiphenyltrichloroethane (DDT) and hexachlorobenzene (HCB) in samples collected at the time of breast cancer discovery, in order to avoid the potential consequences of body weight change ( after chemotherapy or radiotherapy) on the pesticide residue levels. Methods: Blood levels of HCB and total DDT (we calculated total DDT concentrations by adding all DDT and DDE isomers) were compared in 159 women with breast cancer and 250 presumably healthy controls. Risk of breast cancer associated with organochlorine concentration was evaluated. Results: Mean levels of total DDT and HCB were significantly higher for breast cancer patients than for controls. No differences in serum levels of total DDT or HCB were found between oestrogen receptor positive and oestrogen receptor negative patients with breast cancer. Conclusions: These results add to the growing evidence that certain persistent pollutants may occur in higher concentrations in blood samples from breast cancer patients than controls. [less ▲]

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See detailLe douglas sur substrat calcaire. Etude de ses potentialités forestières en Calestienne
Mouchet, F.; Claessens, Hugues ULiege; Laroche, J.

in Forêt Wallonne (2003), (64), 27-32

Pour la sylviculture, la principale caractéristique de la Calestienne est très certainement la combinaison entre sa haute biodiversité et sa faible aptitude à la production de bois. Ainsi, la difficulté ... [more ▼]

Pour la sylviculture, la principale caractéristique de la Calestienne est très certainement la combinaison entre sa haute biodiversité et sa faible aptitude à la production de bois. Ainsi, la difficulté de trouver une essence économiquement rentable pouvant valoriser des sols souvent très ingrats a poussé plusieurs générations de forestiers à effectuer de nombreux essais sylviculturaux. Parmi ceux-ci, la célèbre introduction du pin noir, et plus récemment et plus discrètement, celle du douglas. [less ▲]

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See detailPrevention secondaire apres syndrome coronarien aigu
Legrand, Victor ULiege; Gach, Olivier ULiege; Martinez, Christophe ULiege

in Revue Médicale de Liège (2003), 58(5), 279-82

Acute coronary syndromes are a major health care problem which remains associated with high rates of mortality, myocardial infarction and rehospitalisation after hospital discharge for acute treatment ... [more ▼]

Acute coronary syndromes are a major health care problem which remains associated with high rates of mortality, myocardial infarction and rehospitalisation after hospital discharge for acute treatment. The general practitioner has a critical role in the management of these patients. Physicians have to ensure an adequate control of all coronary risk factors. Systematic use of statines and aspirin is recommended. Also, the use of clopidogrel, fibrates, beta blockers and ACE inhibitors should be considered in some circumstances. Conversely, hormone replacement therapy in women, antioxydants and nitrates in the absence of angina are ineffective. [less ▲]

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See detailA micellar route to ordered arrays of magnetic nanoparticles: From size-selected pure cobalt dots to cobalt-cobalt oxide core-shell systems
Boyen, Hans-Gerd; Kästle, Gerd; Zürn, Klaus et al

in Advanced Functional Materials (2003), 13(5), 359-364

Starting with Co-salt-loaded inverse micelles, which form if the diblock copolymer polystyrene-block-poly(2-vinylpyridine) is dissolved in a selective solvent like toluene and CoCl2) is added to the ... [more ▼]

Starting with Co-salt-loaded inverse micelles, which form if the diblock copolymer polystyrene-block-poly(2-vinylpyridine) is dissolved in a selective solvent like toluene and CoCl2) is added to the solution, monomicellar arrays of such micelles exhibiting a significant hexagonal order can be prepared on top of various substrates with tailored intermicellar distances and structure heights. In order to remove the polymer matrix and to finally obtain arrays of pure Co nanoparticles, the micelles are first exposed to an oxygen plasma, followed by a treatment in a hydrogen plasma. Applying in-situ X-ray photoelectron spectroscopy, it is demonstrated that: 1) The oxygen plasma completely removes the polymer, though conserving the original order of the micellar array. Furthermore, the resulting nanoparticles are entirely oxidized with a chemical shift of the Co 2P(3/2) line pointing to the formation Of Co3O4. 2) By the subsequent hydrogen plasma treatment the nanoparticles are fully reduced to metallic Co. 3) By exposing the pure Co nanoparticles for 100 s to various oxygen partial pressures p(O2), a stepwise oxidation is observed with a still metallic Co core surrounded by an oxide shell. The data allow the extraction of the thickness of the oxide shell as a function of the total exposure to oxygen (p(O2) x time), thus giving the opportunity to control the ferromagnetic-antiferromagnetic composition of an exchange-biased magnetic system. [less ▲]

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See detailPrevention of malnutrition in older people during and after hospitalisation: results from a randomised controlled clinical trial
Gazzotti, C.; Arnaud-Battandier, F.; Parello, M. et al

in Age & Ageing (2003), 32(3), 321-325

Objective: to prevent the occurrence of weight loss during hospitalisation and following discharge by daily oral supplementation. Design: in a prospective, randomised, controlled study of 80 patients aged ... [more ▼]

Objective: to prevent the occurrence of weight loss during hospitalisation and following discharge by daily oral supplementation. Design: in a prospective, randomised, controlled study of 80 patients aged 75 or more, and at risk of undernutrition based on their initial Mini Nutritional Assessment score, patients were randomised into a control group or one receiving oral supplementation. The intervention was a prescription of 200 ml sweet or salty sip feed twice daily (500 kcal, 21 g protein per day) throughout hospitalisation and convalescence. Nutritional status was assessed at baseline and after 2 months using Mini Nutritional Assessment and body weight record. Results: compliance with oral supplementation was good and daily extra energy intake was 407+/-184 kcal. On day 60, significant weight loss from upon admission was observed in the control group (-1.23+/-2.5 kg; P=0.01), but not in the supplemented group (0.28+/-3.8 kg; NS). At the end of the study, Mini Nutritional Assessment scores were higher in the supplemented group than in the control group (23.5+/-3.9 versus 20.8+/-3.6; P<0.01). Conclusion: use of daily oral supplementation during and after hospitalisation maintains body weight and increases Mini Nutritional Assessment score in patients at risk of undernutrition. [less ▲]

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See detailLe point sur la maladie du hêtre en Wallonie
Huart, O.; De Proft, M.; Grégoire, J. C. et al

in Forêt Wallonne (2003), (64), 2-20

Le hêtre, troisième essence feuillue (en volume) de Wallonie, est actuellement victime, essentiellement en Ardenne et en Gaume, d’un important problème sanitaire mettant en jeu des attaques d’insectes et ... [more ▼]

Le hêtre, troisième essence feuillue (en volume) de Wallonie, est actuellement victime, essentiellement en Ardenne et en Gaume, d’un important problème sanitaire mettant en jeu des attaques d’insectes et de champignons, communément appelé « maladie du hêtre ». Les premiers symptômes inquiétants du phénomène ont été identifiés à l’automne 1999, puis se sont considérablement amplifiés en 2000 et surtout en 2001, et se sont accompagnés de dégâts très importants et sans précédent connu. La nature et la dynamique d’apparition des symptômes observés suggéraient d’emblée qu’il s’agissait d’un phénomène inhabituel et apparemment distinct des dépérissements « classiques » du hêtre, bien que la possibilité d’interactions entre ces deux problèmes ne puisse être écartée a priori. L’ampleur de la zone géographique au sein de laquelle les symptômes se sont déclarés de manière relativement brusque et simultanée constitue également une caractéristique remarquable du phénomène observé. [less ▲]

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See detailDépistage et détection précoce des cancers : Pourquoi? Comment? Lesquels? Qui et où? A quel prix?
Castronovo, Vincenzo ULiege

in Revue Médicale de Liège (2003), 58(5), 310-3

Cancer is fighting with cardiovascular diseases for the first place as the number one killer of humans living in industrialized countries. Several factors contribute to the high incidence of cancers. The ... [more ▼]

Cancer is fighting with cardiovascular diseases for the first place as the number one killer of humans living in industrialized countries. Several factors contribute to the high incidence of cancers. The spectacular increase in life expectancy of individuals living in these areas is certainly one of the major causes that explain the steady rise of the number of patients suffering and dying from cancer. For most cancers, when the issue is fatal, it is usually after a long morbidity period often very difficult for the patient and with heavy public health consequences. Thus, a major challenge that the health professionals are facing is the reduction of malignant disease incidence. Primary prevention would be certainly the most successful strategy. However, its implementation is of extreme difficulty. Secondary prevention, non-exclusive to the primary one, represents probably the best approach to decrease morbidity and mortality linked to these diseases. Screening and early diagnosis are the two axes of such a strategy. The first one concerns the detection of a malignant lesion in an asymptomatic patient. Early diagnosis implies to consider with attention and explore any signs or symptoms that can be easily missed by the patient or the doctor and, hence, could be the first indication of a progressing cancer. The impact of cancer screening on the mortality rate due to cancer is still the subject of intense debates, while the efficacy of early diagnosis is unanimously recognized. Still, it is cancer screening that is the center of most attentions.... [less ▲]

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See detailReproductibilité de la mesure de la fraction d'éjection ventriculaire gauche par ventriculographie isotopique lors d'un changement de caméra
Seret, Alain ULiege; Lisart, Jean; Blocklet, Didier

in Médecine Nucléaire : Imagerie Fonctionnelle et Métabolique (2003), 27(5), 251-256

The reproducibility of the left ventricular ejection fraction measure by equilibrium radionuclide angiocardiography has been evaluated when a large field of view camera is used instead of the small field ... [more ▼]

The reproducibility of the left ventricular ejection fraction measure by equilibrium radionuclide angiocardiography has been evaluated when a large field of view camera is used instead of the small field of view that has been used for 15 years in the service. The results have been analyzed using correlation curves, the Bland-Altman method and a paired t test. Linear regression between the left ejection fraction is FEVG(large field of view) = 0.961 FEVG(small field of view) + 5.0 (r = 0.935). Intra-operator reproducibility (mean difference = - 0.15 ± 2.02) and inter-operator reproducibility (absolute value of the mean difference smaller than 1.1 and standard deviation smaller that 2.5) have been measured for the 4 nurses that are entitled to practice this examination in the case of the large field of view camera. Finally, the reproducibility between two different acquisitions with the large field of view camera has been investigated and the following result has been obtained: FEVG(2) = 0.955 FEVG(1) + 3.178 (r = 0.948). Therefore, the reproducibility of the left ejection fraction measure was estimated to be within the expected limits and no correction for the camera change is applied to the long-term follow-up of the patients. [less ▲]

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See detailPrevention cardiovasculaire chez la personne agee
Lebois, Florence ULiege; Allepaerts, Sophie ULiege; Lancellotti, Patrizio ULiege et al

in Revue Médicale de Liège (2003), 58(5), 275-8

The aging of the cardiovascular system leads to an increased incidence of hypertension, coronary artery disease and ischemic stroke. Since cardiovascular disease is the leading cause of morbidity and ... [more ▼]

The aging of the cardiovascular system leads to an increased incidence of hypertension, coronary artery disease and ischemic stroke. Since cardiovascular disease is the leading cause of morbidity and mortality in patients > or = 70 years old, prevention seems crucial for improving quality of life and reducing medical costs. In this article we present some aspects of cardiovascular prevention in elderly patients. [less ▲]

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See detailSubchondral tibial bone mineral density predicts future joint space narrowing at the medial femoro-tibial compartment in patients with knee osteoarthritis
Bruyère, Olivier ULiege; Dardenne, Charles-Bernard ULiege; Lejeune, Eric ULiege et al

in BONE (2003), 32(5), 541-545

Preliminary studies have shown that dual-energy X-ray absorptiometry (DXA) produces images of sufficient quality for a precise and accurate measurement at density of the subchondral bone. The objective of ... [more ▼]

Preliminary studies have shown that dual-energy X-ray absorptiometry (DXA) produces images of sufficient quality for a precise and accurate measurement at density of the subchondral bone. The objective of this study was to investigate the relationship between baseline subchondral tibial bone mineral density (BMD) and joint space narrowing observed after 1 year at the medial femoro-tibial compartment of the knee joint. Fifty-six consecutive patients, from both genders, with knee osteoarthritis diagnosed according to the American College of Rheumatology criteria, were included in the study. Radiographic posteroanterior views were taken, at baseline and after 1 year of follow-up. Minimum joint space width (JSW) measurement, at the medial femoro-tibial joint, was performed with a 0.1-mm graduated magnifying lens. Baseline BMD of the subchondral tibial bone was assessed by DXA. The mean +/- SD age of the patients was 65.3 +/- 8.7 years, with a body mass index of 28.0 +/- 4.9 kg/m(2). The minimum JSW was 3.5 +/- 1.5 mm and the mean BMD of the subchondral bone was 0.848 +/- 0.173 g/cm(2). There was a significant negative correlation between subchondral BMD and 1-year changes in minimum JSW (r = -0.43, p = 0.02). When performing a multiple regression analysis with age, sex, body mass index, and minimum JSW at baseline as concomitant variables, BMD of the subchondral bone as well as JSW at baseline were independent predictors of 1-year changes in JSW (p = 0.02 and p = 0.005, respectively). Patients in the lowest quartile of baseline BMD (<0.73 g/cm(2)) experienced less joint space narrowing than those in the highest BMD quartile (>0.96 g/cm(2)) (+0.61 +/- 0.69 turn versus -0.13 +/- 0.27 mm; p = 0.03). Assessment of BMD of the subchondral tibial bone is significantly correlated with future joint space narrowing and could be used as a predictor of knee osteoarthritis progression. (C) 2003 Elsevier Science (USA). All rights reserved. [less ▲]

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See detailDimerization and DNA binding properties of the Bacillus licheniformis 749/I BlaI repressor
Filée, Patrice ULiege; Vreuls, Christelle ULiege; Herman, Raphaël ULiege et al

in Journal of Biological Chemistry (2003), 278(19), 16482-16487

In the absence of penicillin, the beta-lactamase encoding gene blaP of Bacillus licheniformis 749/I is negatively regulated by the transcriptional repressor BlaI. Three palindromic operator regions are ... [more ▼]

In the absence of penicillin, the beta-lactamase encoding gene blaP of Bacillus licheniformis 749/I is negatively regulated by the transcriptional repressor BlaI. Three palindromic operator regions are recognized by BlaI: two in the blaP promoter (OP1 and OP2) and one (OP3) in the promoter of the blaI-blaR1 operon. In this study, the dissociation constant of the purified BlaI dimer was estimated at 25 muM by equilibrium ultracentrifugation. Quantitative Western blot analysis indicates that the intracellular concentrations of BlaI in B. licheniformis 749/I and Bacillus subtilis transformed by a multicopy plasmid harboring the beta-lactamase locus (blaP-blaI-blaR1) were lower than (1.9 muM) or in the same range as (75 muM) the dissociation constant, respectively. This suggests that BlaI is partially dimeric in the cytoplasm of these strains and interacts in vivo with its operators as a preformed dimer. This hypothesis is supported by band shift assays on an operator containing a randomized half-operator sequence. The global dissociation constants of the operator-BlaI dimer complexes were measured by band shift assays and estimated as K-dOP1=1.7+/-0.5 10(-15) M-2, K-dOP2=3.3+/-0.9 10(-15) M-2, and K-dOP3=10.5+/-2.5 10(-15) M-2. The role of the DNA binding properties of BlaI on the beta-lactamase regulation is discussed. [less ▲]

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See detailNew strategy for comprehensive analysis of polybrominated diphenyl ethers, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls by gas chromatography coupled with mass spectrometry
Pirard, Catherine ULiege; De Pauw, Edwin ULiege; Focant, Jean-François ULiege

in Journal of Chromatography. A (2003), 998(1-2), 169-181

A strategy for determination of polybrominated diphenyl ethers, polychlorinated dibenzodioxins, polychlorinated dibenzofurans and polychlorinated biphenyls on fatty matrices has been established. After ... [more ▼]

A strategy for determination of polybrominated diphenyl ethers, polychlorinated dibenzodioxins, polychlorinated dibenzofurans and polychlorinated biphenyls on fatty matrices has been established. After extraction, the proposed method allows the purification and the fractionation of all target groups of compounds in a simple multi-step automated clean-up. Furthermore, their subsequent analysis is carried out using a single benchtop mass spectrometer, in four separate injections. Required sensitivity considering levels found in the environment is attained using electron impact ionisation followed by tandem in time mass spectrometry. The whole method has been evaluated on standard solution and quality control samples consisting of fortified beef fat. Sensitivity, selectivity, accuracy and repeatability were tested with satisfactory results. (C) 2003 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailComparaison de l'evolution des marqueurs biologiques du remodelage osseux apres six mois de traitement hormonal substitutif par 17 beta-estradiol cutane ou estrogenes sulfoconjugues equins et acetate de nomegestrol
Collette, Julien ULiege; Viethel, P.; Dethor, M. et al

in Gynécologie Obstétrique & Fertilité (2003), 31(5), 434-41

OBJECTIVE: To compare changes in biochemical markers of bone turnover in postmenopausal women who received sequential discontinuous hormone replacement therapy (HRT) with either transdermal 17 beta ... [more ▼]

OBJECTIVE: To compare changes in biochemical markers of bone turnover in postmenopausal women who received sequential discontinuous hormone replacement therapy (HRT) with either transdermal 17 beta-estradiol gel (group 1) or oral equine sulfoconjugated estrogen (group 2), plus nomegestrol acetate. PATIENTS AND METHOD: Prospective, open, randomized, controlled trial, conducted on 3 parallel groups of 106 postmenopausal women. All treated groups received estrogen therapy for 25 consecutive days every month. The estrogen used was either 1.5 mg/day of transdermal 17 beta-estradiol gel (group 1) [N = 42, average age (AA) = 51.6 years, average duration of menopause (ADM = 21.5 months)], or 0.625 mg/day of oral equine sulfoconjugated estrogen (group 2) [N = 39, AA = 51.3 years, ADM = 16.8 months]. In all cases nomegestrol acetate 5 mg/day was added for 12 consecutive days every month. The control group comprised 25 patients, [AA = 53.4 years, ADM = 33.7 months]. Two bone resorption markers: urinary cross-linked N-telopeptide and C-telopeptide of type I collagen (U-NTX/Cr, U-CTX/Cr), and a bone formation marker: serum bone specific alkaline phosphatase activity were measured before and 6 months after treatment start. RESULTS: Significant decreases from baseline values were observed for the 3 biochemical markers in both treated groups compared with control (P < 0.001). There were no significant differences in changes between the 2 treated groups for the 3 biochemical markers. The mean percentage change in the 3 biochemical markers was: from -9.3 to -45.5% in group 1, from -20.5 to -39% in group 2, and from -3.3 to 2% in control group. In group 1, the mean percentage decreases in U-CTX reached optimal threshold of bone turnover change (-45%) which is considered by the International Osteoporosis Foundation as clinically relevant because it predicts an increase in BMD greater than 3% when treatment is maintained over a long term. DISCUSSION AND CONCLUSION: Both treated groups induced a significant comparable decrease of bone turnover markers after 6 months of intervention, compared with control. The group treated with cyclic administration of transdermal 17 beta-estradiol (1.5 mg/day) and nomegestrol acetate (5 mg/day) showed a bone resorption markers decrease corresponding to the threshold of clinical relevance described in the international literature and predictive of positive BMD response in long term. [less ▲]

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See detailDepistage des cancers du sein et du col de l'uterus: attitudes et comportements de la population feminine liegeoise
Escoyez, Bernadette; Mairiaux, Philippe ULiege

in Revue Médicale de Liège (2003), 58(5), 319-26

Several surveys have shown that in Belgium the coverage of the female population for both breast and cervix cancers screening is too low and not evenly distributed in the target population. In order to ... [more ▼]

Several surveys have shown that in Belgium the coverage of the female population for both breast and cervix cancers screening is too low and not evenly distributed in the target population. In order to highlight modifiable behavioural factors about cancer screening and to know the profile of women who exhibit an inadequate preventive behaviour, a postal survey has been conducted in the city of Liege. A self-administrated questionnaire was sent to a randomised sample of 1.000 women from two age groups (25-37 and 45-57 years); 444 women returned the questionnaire (response rate: 45.4%). The results show that the responders who do not comply with the recommendations for cancer screening are more often unemployed women with a low educational level, women who have a poor perception of their health, or women who are not regularly followed by a gynaecologist. Those women have more often poor knowledge and wrong beliefs about prevention, a negative perception of the curability of a cancer detected early and of the efficacy of screening tests. In spite of an overrepresentation of women with an university degree among the responders, it was also observed that more than 1 woman out of 4 in the 25-37 years age group is not convinced of the pap smear efficacy. In view of these results, the design of screening campaigns for cancer prevention has to involve specific efforts targeted at under-privileged subgroups within the female population. [less ▲]

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See detailRole of the thymus in the development of tolerance and autoimmunity towards the neuroendocrine system
Geenen, Vincent ULiege; Brilot, Fabienne

in Annals of the New York Academy of Sciences (2003), 992

The thymus is the unique lymphoid organ inside which a confrontation occurs throughout life between neuroendocrine self-antigens and a recently evolved system with original recombination machinery driving ... [more ▼]

The thymus is the unique lymphoid organ inside which a confrontation occurs throughout life between neuroendocrine self-antigens and a recently evolved system with original recombination machinery driving random generation of immune response diversity. Through transcription of neuroendocrine genes in the thymus stromal network and expression of cognate receptors by immature T cells, the neuroendocrine system regulates early T cell differentiation. In addition and more specifically, intrathymic presentation of neuroendocrine self-antigens by, or in close association with, major histocompatibility complex (MHC) proteins is responsible for the establishment of central immune self-tolerance of neuroendocrine principles. All members of the insulin gene (INS) family are expressed in the thymus stroma according to a precise hierarchy and cell topography: IGF2 (thymic epithelial cells) > IGF1 (thymic macrophages) much greater than INS (thymic medullary epithelial cells and/or dendritic cells). Given this hierarchical pattern in gene expression, the protein IGF-2 is more tolerated than INS. Igf2 transcription is defective in the thymus of bio-breeding (BB) rat, one animal model of type 1 diabetes (T1DM). This thymus-specific defect in Igf2 expression may explain both the absence of central tolerance to INS-secreting beta cells and the lymphopenia (including lack of regulatory RT6(+) T cells) in diabetes-prone BB rats. INS B:9-23 and the homologous sequence of IGF-2 compete for binding to DQ8, an MHC class II allele conferring major susceptibility to T1DM. In young DQ8(+) T1DM patients, INS B:9-23 presentation by DQ8 elicits a dominant IFN-gamma secretion by isolated PBMCs, whereas presentation of the IGF-2 self-antigen promotes a dominant regulatory interleukin-10 secretion. These data demonstrate that opposite immune responses are driven by MHC presentation of a self-antigen (here, IGF-2) and an autoantigen (INS, as "altered" self). The important tolerogenic properties of thymic self-antigens deserve now to be exploited for prevention and/or cure of devastating autoimmune diseases such as T1DM. [less ▲]

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See detailRestricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts adjacent to human breast cancer cells
Bisson, C.; Blacher, Silvia ULiege; Polette, M. et al

in International Journal of Cancer = Journal International du Cancer (2003), 105(1), 7-13

The membrane type-1 matrix metalloproteinase (MT1-MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to activate other MMPs (MMP-2 and MMP-13) and to degrade ... [more ▼]

The membrane type-1 matrix metalloproteinase (MT1-MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to activate other MMPs (MMP-2 and MMP-13) and to degrade extracellular matrix. Our study was undertaken to localize and identify the MT1-MMP expressing cells in human breast adenocarcinomas. A textural analysis of images obtained by immunohistochemistry and in situ hybridization showed precisely the co-expression of alpha smooth muscle actin (alphaSM actin) and MT1-MMP in myofibroblasts. MT1-MMP expression is confined to myofibroblasts in close contact with tumor cells. In sharp contrast, the expression of MMP-2 was more widely distributed in both alphaSM actin positive and negative cells close to and at distance from cancer cell clusters. Our in vitro observations are consistent with the higher level of MT1-MMP expression and of MMP-2 activation observed in alphaSM actin positive fibroblasts derived from breast tumors, as compared to normal breast fibroblasts. Collectively, these results implicate myofibroblasts as major producer of MT1-MMP in breast cancer and emphasize the importance of stromal-epithelial cell interactions in their progression. [less ▲]

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See detailLe role de la chirurgie dans la prevention d'un accident vasculaire cerebral d'origine carotidienne
Limet, Raymond ULiege

in Revue Médicale de Liège (2003), 58(5), 293-6

Early and late results of North American Trial (NASCET) demonstrate the efficacy of surgery to prevent major ipsilateral stroke in symptomatic patients; the benefit is proportional to the degree of ... [more ▼]

Early and late results of North American Trial (NASCET) demonstrate the efficacy of surgery to prevent major ipsilateral stroke in symptomatic patients; the benefit is proportional to the degree of stenosis. In asymptomatic patients, the benefit is real, but quantitavivement less pronounced. [less ▲]

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See detailLe depistage du cancer du col de l'uterus en Belgique: le point en 2003
Delvenne, Philippe ULiege; Jacobs, Nathalie ULiege; Lambert, Chantal ULiege et al

in Revue Médicale de Liège (2003), 58(5), 316-8

Squamous cell cancer of the uterine cervix is associated with a high morbidity and mortality worldwide and in Belgium. Its development is related to the infection by oncogenic human papillomavirus (HPV ... [more ▼]

Squamous cell cancer of the uterine cervix is associated with a high morbidity and mortality worldwide and in Belgium. Its development is related to the infection by oncogenic human papillomavirus (HPV) types. Cervical cancer is preceded by dysplasic stages (squamous intraepithelial lesions) which can be detected by a Pap smear. The goal of this paper is to review the screening of uterine cervical cancer in Belgium. [less ▲]

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See detailOrganising access to evidence-based medicine resources on the web
Delvenne, Catherine ULiege; Pasleau, Françoise ULiege

in Computer Methods & Programs in Biomedicine (2003), 71(1), 1-10

The continuing education is a challenge for health care professionals, considering the growing amount and variable quality of information in this field. In this context, we developed a method allowing ... [more ▼]

The continuing education is a challenge for health care professionals, considering the growing amount and variable quality of information in this field. In this context, we developed a method allowing clinicians to have a centralised access to the best current medical evidence supporting medical decision-making. Relevant data has been gathered according to the rules stated for Evidence-Based Medicine (EBM) and organised in a free-of-charge Web site, created by using common software applications. The general aim of our study was to encourage individual practice of EBM by providing tutorials as well as a selection of free access tools for searching medical information on the Web. In addition to the education part, we provided clinicians, through a Web interface, with up-to-date, accurate and quality data obtained from heterogeneous sources and presented in a full text format when available. The developed methodology has been applied to human prostate cancer. URL address: http://www.ebm.lib.ulg.ac.be/prostate/index.htm. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailOral administration of the growth hormone (GH) secretagogue NN703 in adult patients with GH deficiency.
Svensson, J.; Monson, J. P.; Vetter, T. et al

in Clinical Endocrinology (2003), 58(5), 572-580

OBJECTIVE: Little is known of the usefulness of GH secretagogues (GHSs) in GH-deficient (GHD) adults. The objective of this study was to determine the number of responders to treatment with NN703 in GHD ... [more ▼]

OBJECTIVE: Little is known of the usefulness of GH secretagogues (GHSs) in GH-deficient (GHD) adults. The objective of this study was to determine the number of responders to treatment with NN703 in GHD adults. DESIGN: A multicentre, randomized, double-blind, and placebo-controlled study. PATIENTS: Ninety-seven GHD adults were included. MEASUREMENTS: The GH response before and after 1 week of oral treatment with NN703 (n = 83) or placebo (n = 14) was determined. The first and last dose of NN703 was 3 mg/kg, whereas the dose of NN703 was 1.5 mg/kg/day during the 6 days between the first and last doses. Furthermore, all 97 patients received 1 micro g/kg GH-releasing hormone (GHRH) 3 weeks after the last dose of NN703. RESULTS: Serum GH peak and area under curve (AUC) values after the first NN703 administration were greater than those after placebo administration (P < 0.05). However, after correction for the lower body mass index (BMI) in the NN703 group, this difference lost statistical significance. After 1 week of therapy, GH peak and AUC values were similar following the final doses of NN703 and placebo. Serum peak and AUC values of other anterior pituitary hormones were similar between the NN703 and placebo groups both after the first and last administration of study drug. Nine of the 83 patients (11%) responded with a serum peak GH concentration >or= 5 micro g/l after the first and/or last NN703 administration, whereas no patient responded after placebo administration. Serum IGF-I was unaffected by 1-week NN703 treatment, whereas serum IGFBP-3 was increased (P < 0.05 vs. placebo) also after correction for BMI. Mean serum peak GH concentration after GHRH administration was 2.1 micro g/l (+/-0.3, SEM), which was higher than that after the first NN703 administration (1.32 +/- 0.3, P < 0.05). CONCLUSION: NN703 administration was generally well tolerated. Eleven per cent of the GHD adult patients responded with a peak GH response >or= 5 micro g/l after the first and/or last administration of oral NN703. Although a majority of GHD adults will not respond to NN703, the present results suggest that oral NN703 treatment could be useful in some adult patients with moderately severe GHD. These patients may be identified by a test dose of GHS. [less ▲]

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See detailQuasi-asymptotic development of conditioned hyperactivity induced by intermittent injections of cocaine in C57BL/6J mice
Brabant, Christian ULiege; Tambour, Sophie ULiege; Tirelli, Ezio ULiege

in Pharmacology, Biochemistry & Behavior (2003), 75(2), 273-280

The emergence of a conditioned cocaine-induced hyperlocomotion was examined in C57BL/6J mice using a procedure that has not been used previously. Two days after a session of preexposure to the test ... [more ▼]

The emergence of a conditioned cocaine-induced hyperlocomotion was examined in C57BL/6J mice using a procedure that has not been used previously. Two days after a session of preexposure to the test chambers under saline, a first group of mice (cocaine-cued) received five once-daily injections of 10-mg/kg sc cocaine every other day (on the odd days of the chronic treatment period) and a saline injection on the 5 days following each cocaine injection day (on the even days of the treatment period), in all cases before being placed in the test chamber. Another group of mice (saline-cued) received 10 injections of saline on both the even and the odd days in the same context, and a third group of mice (cocaine-uncued) received five injections of saline on the even days in the test context and five injections of cocaine on the odd days in an alternative context. On the odd days sessions, the cocaine-cued group showed significant repeated increases in locomotion without behavioural sensitisation being induced, whereas the saline-cued levels of locomotion remained on baseline levels. On the first even session, the three groups did not differ from each other and showed lower levels of locomotion than on the preexposure session. During the two following even sessions, the cocaine-cued group showed an increase in locomotion that levelled off on the two remaining sessions, whereas the saline-cued and the cocame-uncued groups (which presented comparable values) exhibited significantly lower levels of locomotion. That pattern of successive placebo responses resembles the typical S-shaped development of a Pavlovian conditioned response, albeit the increase described here was quite rapid. The protocol used here may provide an additional method for the experimental analysis of stimulant-induced conditioned placebo activity. (C) 2003 Elsevier Science Inc. All rights reserved. [less ▲]

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See detailBiochemical markers of bone and cartilage remodeling in prediction of longterm progression of knee osteoarthritis
Bruyère, Olivier ULiege; COLLETTE, Julien ULiege; Ethgen, Olivier ULiege et al

in Journal of Rheumatology (2003), 30(5), 1043-50

OBJECTIVE: To investigate the relationship between biochemical markers of bone and cartilage remodeling and severity or progression (symptoms and structure) of knee osteoarthritis (OA). METHODS: Mean and ... [more ▼]

OBJECTIVE: To investigate the relationship between biochemical markers of bone and cartilage remodeling and severity or progression (symptoms and structure) of knee osteoarthritis (OA). METHODS: Mean and minimal joint space width (JSW) of the femorotibial joint were measured from standardized radiographs taken at baseline and at the end of a 3-year longitudinal study of patients with knee OA. Pain, stiffness, and physical function subscales of the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index were assessed at the same time points. Biochemical markers [serum keratan sulfate (KS), serum hyaluronic acid (HA), urine pyridinoline (PYD) and deoxypyridinoline (DPD), serum osteocalcin (OC), cartilage oligomeric matrix protein (COMP)] were assessed at baseline and after 1 year. RESULTS: At baseline, no significant correlations were observed between values of biochemical markers and JSW or any of the WOMAC scores. Baseline markers were not correlated with 3-year percentage changes observed in mean or minimal JSW and WOMAC scores. Changes observed after 1 year in OC and HA were significantly correlated with 3-year progression in mean JSW (r = -0.24, p = 0.04 and r = 0.27, p = 0.02, respectively) and in minimal JSW (r = -0.31, p = 0.01 and r = 0.24, p = 0.04, respectively). In patients from the lowest quartile of 1-year changes in HA (< -21.22 ng/ml), mean JSW decreased after 3 years by 0.76 (1.23) mm compared to an increase of 0.11 (0.83) mm in patients in the highest quartile (> +14.34 ng/ml) (p = 0.03). CONCLUSION: The 3-year radiological progression of knee OA could be predicted by a 1-year increase in OC or a 1-year decrease in HA levels. [less ▲]

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See detailValidation des procédures analytiques quantitatives, Harmonisation des démarches
Hubert, Philippe ULiege; Nguyen-Huu, J. J.; Boulanger, Bruno ULiege et al

in STP Pharma Pratiques (2003), 13(3), 101-138

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See detailAn improved radioimmunoassay for measurement of pepsinogen in porcine blood samples
Banga-Mboko, Henri; Sulon, Joseph ULiege; Lange, J. M. et al

in Veterinary Journal (2003), 165(3), 288-295

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen ... [more ▼]

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen concentrations in serum samples from fetuses and pigs of different ages. Compared to a previously described RIA, major improvements were made concerning the use of specific polyclonal antibodies and the use of an appropriate buffer. The assay was able to detect pepsinogen concentrations of >/=0.2 ng/mL. The recovery of pepsinogen was close to 95%. The intra-assay coefficients of variations ranged between 3.9 and 7.5% whereas the interassay ranged between 8.8 and 11.9%. These percentages correspond to a satisfactory accuracy and reproducibility of the assay. No cross-reactions were observed with the main commercially available products of the aspartic proteases family except porcine pepsin cross-reacted over 62.5 microg/mL. Pepsinogen concentrations increased steadily with increasing age of the fetuses and the pigs (P<0.05). Pepsinogen concentrations (+/-SE) in fetuses of 90-100 (n=24) and 100-110 days of pregnancy (n=36) were 0.5+/-0.1 and 5.3+/-1.3 ng/mL, respectively. In pigs of 21, 98, and 213 days of age, the pepsinogen concentrations were 290.6+/-10.8, 343.1+/-17.9 and 383.5+/-15.3 ng/mL, respectively. The results demonstrate that RIA is accurate and can be used easily to assess pepsinogen concentrations in pig sera. The test may constitute a valuable tool in epidemiological surveys and in studies related to gastric diseases in pigs. [less ▲]

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See detailEarly pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests
Karen, A.; Beckers, Jean-François ULiege; Sulon, Joseph ULiege et al

in Theriogenology (2003), 59(9), 1941-1948

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 ... [more ▼]

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 Awassi x Merino ewes and blood samples were collected at Days 0 (day of the insemination), 18, 22, 29, 36, and 50 after artificial insemination (AI). Plasma P4 concentrations at Days 0 and 18 were determined by double antibody radioimmunoassay, while PAG concentrations at Days 22, 29, 36 and 50 were determined by a heterologous, double-antibody radioimmunoassay (RIA) using the bovine PAG 67 kDa subunit as tracer and standard and rabbit antiserum raised against a mixture of caprine 55 and 59 kDa PAG subunits as the first antibody. The discriminatory value for diagnosis of pregnancy by the P4 and the PAG-RIA tests was greater than or equal to1 ng/ml. Based on lambing data, the accuracy for diagnosing pregnant (sensitivity) and non-pregnant ewes (specificity) and predictivity of both tests were calculated. The sensitivity, specificity, positive and negative predictive values for P4 and PAG tests were 100, 95.4, 81.6, and 100% at Day 18 (P4) and 93.5, 100, 100 and 98.7% at Day 22 (PAG), respectively. For diagnosis of non-pregnant ewes the PAG test had significantly higher specificity than the P4 test (P < 0.05). It is concluded that ovine pregnancy can be reliably diagnosed at Day 22 after AI by using a heterologous radioimmunoassay of PAG. [less ▲]

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See detailEffects of background electrolyte composition and addition of selectors on separation selectivity in nonaqueous capillary electrophoresis
Fillet, Marianne ULiege; Servais, Anne-Catherine ULiege; Crommen, Jacques ULiege

in Electrophoresis (2003), 24(10), 1499-1507

This review gives a survey of the approaches employed to obtain, enhance and tune selectivity in nonaqueous capillary electrophoresis (NACE). Recent developments in NACE are described and the effects of ... [more ▼]

This review gives a survey of the approaches employed to obtain, enhance and tune selectivity in nonaqueous capillary electrophoresis (NACE). Recent developments in NACE are described and the effects of background electrolyte composition and addition of selectors on separation selectivity are discussed. The use of one organic solvent, a mixture of several organic solvents or the use of additives to tune separation selectivity in NACE is presented and a list of relevant applications is included. [less ▲]

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See detailLong-term trends of inorganic chlorine from ground-based infrared solar spectra: Past increases and evidence for stabilization
Rinsland, Curtis P.; Mahieu, Emmanuel ULiege; Zander, Rodolphe ULiege et al

in Journal of Geophysical Research (2003), 108(D8), 4252

Long-term time series of hydrogen chloride (HCl) and chlorine nitrate (ClONO2) total column abundances has been retrieved from high spectral resolution ground-based solar absorption spectra recorded with ... [more ▼]

Long-term time series of hydrogen chloride (HCl) and chlorine nitrate (ClONO2) total column abundances has been retrieved from high spectral resolution ground-based solar absorption spectra recorded with infrared Fourier transform spectrometers at nine NDSC (Network for the Detection of Stratospheric Change) sites in both Northern and Southern Hemispheres. The data sets span up to 24 years and most extend until the end of 2001. The time series of Cly (defined here as the sum of the HCl and ClONO2 columns) from the three locations with the longest time-span records show rapid increases until the early 1990s superimposed on marked day-to-day, seasonal and inter-annual variability. Subsequently, the buildup in Cly slows and reaches a broad plateau after 1996, also characterized by variability. A similar time evolution is also found in the total chlorine concentration at 55 km altitude derived from Halogen Occultation Experiment (HALOE) global observations since 1991. The stabilization of inorganic chlorine observed in both the total columns and at 55 km altitude indicates that the near-global 1993 organic chlorine (CCly) peak at the Earth’s surface has now propagated over a broad altitude range in the upper atmosphere, though the time lag is difficult to quantify precisely from the current data sets, due to variability. We compare the three longest measured time series with two-dimensional model calculations extending from 1977 to 2010, based on a halocarbon scenario that assumes past measured trends and a realistic extrapolation into the future. The model predicts broad Cly maxima consistent with the long-term observations, followed by a slow Cly decline reaching 12–14% relative to the peak by 2010. The data reported here confirm the effectiveness of the Montreal Protocol and its Amendments and Adjustments in progressively phasing out the major man-related perturbations of the stratospheric ozone layer, in particular, the anthropogenic chlorine-bearing source gases. [less ▲]

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See detailInterest of biochemical markers of bone turnover for long-term prediction of new vertebral fracture in postmenopausal osteoporotic women
Bruyère, Olivier ULiege; Collette, Julien ULiege; Delmas, Pierre et al

in Maturitas (2003), 44(4), 259-265

Objective: To analyse the interest of baseline levels and short-term (3-months) changes in serum osteocalcin (BGP), serum bone-specific alkaline phosphatase (BALP) and urinary C-telopeptide of type I ... [more ▼]

Objective: To analyse the interest of baseline levels and short-term (3-months) changes in serum osteocalcin (BGP), serum bone-specific alkaline phosphatase (BALP) and urinary C-telopeptide of type I collagen/creatinine ratio (U-TX) to predict 3-years changes in bone mineral density (BMD) and spinal deformity index (SDI) in postmenopausal osteoporotic women. Methods: Data were derived from a cohort of 603 osteoporotic women corresponding to the placebo arm of a 3-years prospective, double-blind study. Results: Baseline values of BALP, BGP and U-CTX were negatively and significantly correlated with baseline spinal BMD. Significant correlations were also observed between the changes in BMD observed after 36 months at the spine and baseline BALP (r = 0.20, P = 0.0001), BGP (r = 0.09, P = 0.05) and U-CTX (r = -0.11, P = 0.02). At 3 years, 71 women (15.9%) showed an increase in their SDI, corresponding to the occurrence of at least one new vertebral deformity. Baseline values of the four bone turnover markers (BTM) were not significantly related to the occurrence of new vertebral deformities. However, when considering the changes in the BTM observed after 3-months of follow-up, BGP (P = 0.003) and U-CTX (P = 0.047) were identified as significant predictors of an increase of SDI. The associated odds ratios (95% confidence interval (CI)) were 10.922 (2.218-53.78) for unit changes of log BGP and 1.369 (1.003-1.867) for unit changes of log U-CTX. The relative risk (RR) (IC 95%) of having a new vertebral fracture over 36 months was 0.31 (0.15-0.65) when being in the lowest quartile of 3-months changes in BGP as compared with the highest. Conclusion: We conclude that two sequential measurements of BGP and U-CTX performed at 3-months intervals could be of interest to identify postmenopausal osteoporotic women with the highest risk to present new vertebral deformities. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailActivity, stability and flexibility in Glycosidases adapted to extreme thermal environments
Collins, T.; Meuwis, Marie-Alice ULiege; Gerday, Charles ULiege et al

in Journal of Molecular Biology (2003), 328(2), 419-428

To elucidate the strategy of low temperature adaptation for a cold-adapted family 8 xylanase, the thermal and chemical stabilities, thermal inactivation, thermodependence of activity and conformational ... [more ▼]

To elucidate the strategy of low temperature adaptation for a cold-adapted family 8 xylanase, the thermal and chemical stabilities, thermal inactivation, thermodependence of activity and conformational flexibility, as well as the thermodynamic basis of these processes, were compared with those of a thermophilic homolog. Differential scanning calorimetry, fluorescence monitoring of guanidine hydrochloride unfolding and fluorescence quenching were used, among other techniques, to show that the cold-adapted enzyme is characterized by a high activity at low temperatures, a poor stability and a high flexibility. In contrast, the thermophilic enzyme is shown to have a reduced low temperature activity, high stability and a reduced flexibility. These findings agree with the hypothesis that cold-adapted enzymes overcome the quandary imposed by low temperature environments via a global or local increase in the flexibility of their molecular edifice, with this in turn leading to a reduced stability. Analysis of the guanidine hydrochloride unfolding, as well as the thermodynamic parameters of irreversible thermal unfolding and thermal inactivation shows that the driving force for this denaturation and inactivation is a large entropy change while a low enthalpy change is implicated in the low temperature activity. A reduced number of salt-bridges are believed to be responsible for both these effects. Guanidine hydrochloride unfolding studies also indicate that both family 8 enzymes unfold via an intermediate prone to aggregation. (C) 2003 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailSelf-organization of water-soluble complexes of a poly(2-vinylpyridinium)-block-poly(ethylene oxide) diblock with fluorinated anionic surfactants
Gohy, Jean-François; Mores, Sandrine; Varshney, Sunil K et al

in Macromolecules (2003), 36(8), 2579-2581

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See detailSynthesis and characterization of random copolyesters of epsilon-caprolactone and 2-oxepane-1,5-dione
Latere Dwan'Isa, Jean-Pierre; Lecomte, Philippe ULiege; Dubois, Philippe ULiege et al

in Macromolecules (2003), 36(8), 2609-2615

2-Oxepane-1,5-dione (OPD) has been synthesized by the Baeyer-Villiger oxidation of 1,4-cyclohexanedione and copolymerized with epsilon-caprolactone. This random copolymerization has been initiated by ... [more ▼]

2-Oxepane-1,5-dione (OPD) has been synthesized by the Baeyer-Villiger oxidation of 1,4-cyclohexanedione and copolymerized with epsilon-caprolactone. This random copolymerization has been initiated by different metal derivatives, i.e., tin octanoate, dibutyltin dimethoxide, and aluminum isopropoxide. Dibutyltin dimethoxide is the preferred initiator, and the course of polymerization is controlled by the competition of the ketone of OPD and the ester of the lactones for coordination to the initiator. Semicrystalline copolymers are formed, whose the randomness has been confirmed by DSC, H-1 NMR, and C-13 NMR analysis. A single melting temperature (Tm) has been observed, which varies regularly and monotonically with the OPD content as result of a cocrystallization phenomenom. The single glass transition temperature (Tg) obeys the Fox equation. [less ▲]

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See detailDynamics of crack opening in a one-dimensional desiccation experiment
Lecocq, N.; Vandewalle, Nicolas ULiege

in Physica A: Statistical Mechanics and its Applications (2003), 321(3-4), 431-441

We used a simple one-dimensional experiment to investigate the dynamical aspects of crack opening that occurs in clay exposed to shrinkage induced by desiccation. The opening rate of single cracks is ... [more ▼]

We used a simple one-dimensional experiment to investigate the dynamical aspects of crack opening that occurs in clay exposed to shrinkage induced by desiccation. The opening rate of single cracks is obtained. A simple model is introduced to account for the observed behavior. Interaction between adjacent cracks is put into evidence and a collective behavior is observed. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailSF6 ground-based infrared solar absorption measurements: long-term trend, pollution events, and a search for SF5CF3 absorption
Rinsland, Curtis P.; Goldman, Aaron; Stephen, T. M. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (2003), 78(1), 41-53

Infrared solar spectra recorded with the Fourier transform spectrometer in the McMath solar telescope complex on Kitt Peak (31.9degreesN latitude, 111.6degreesW, 2.09 km altitude), southwest of Tucson ... [more ▼]

Infrared solar spectra recorded with the Fourier transform spectrometer in the McMath solar telescope complex on Kitt Peak (31.9degreesN latitude, 111.6degreesW, 2.09 km altitude), southwest of Tucson, Arizona, have been analyzed to retrieve average SF6 tropospheric mixing ratios over a two-decade time span. The analysis is based primarily on spectral fits to absorption by the intense, unresolved nu(3) band Q branch at 947.9 cm(-1). A best fit to measurements recorded with SF6 near typical background concentrations yields a SF6 increase in the average tropospheric mixing ratio from 1.13 pptv (10(-12) per unit volume) in March 1982 to 3.77 pptv in March 2002. The long-term increase by a factor of 3.34 over the time span is consistent with the rapid growth of surface mixing ratios measured in situ at Northern Hemisphere remote stations, though the infrared measurements show a large scatter. Average tropospheric mixing ratio enhancements above background by 2-3 orders of magnitude have been identified in spectra recorded on 5 days between November 1988 and April 1997. These spectra were individually analyzed in an attempt to detect the strongest 8-12 mum band of SF5CF3, a molecule recently identified with an atmospheric growth that has closely paralleled the rise in SF6 during the past three decades. Absorption by the strongest SF5CF3 band was predicted to be above the noise level in the Kitt Peak spectrum with the highest average mean tropospheric SF6 mixing ratio, assuming the reported atmospheric SF5CF3/SF6 ratio and a room temperature absorption cross sections reported for the SF5CF3 903-cm(-1) band. An upper limit of 8 x 10(15) molecules cm(-2) for the SF5CF3 total column was estimated for this case. We hypothesize that the highly elevated SF6 levels above Kitt Peak resulted from a local release experiment rather than production via electrochemical fluoridation of intermediate products, the proposed source of atmospheric SF5CF3. The absence of the SF5CF3 feature in the spectra with elevated SF6 is consistent with the absence of SF5CF3 reported in a pure SF6 sample. Published by Elsevier Science Ltd. [less ▲]

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See detailComplexation of uranyl ions by polypyrrole doped by sulfonated and phosphonated polyethyleneimine
Leroy, D.; Martinot, Lucien; Mignonsin, P. et al

in Journal of Applied Polymer Science (2003), 88(2), 352-359

Branched polyethyleneimine (PEI) was sulfonated by reaction with chloropropanesulfonylchloride and phosphonated by reaction with phosphorous acid and formaldehyde. The accordingly formed polyanions were ... [more ▼]

Branched polyethyleneimine (PEI) was sulfonated by reaction with chloropropanesulfonylchloride and phosphonated by reaction with phosphorous acid and formaldehyde. The accordingly formed polyanions were used as doping agents for polypyrrole (PPy). The amount of doping polyanions into thin films of PPy was measured by Rutherford back-scattering. These films were tested for their capacity to extract uranyl ions from liquid wastes of low level activity. The uranium content was determined by neutron activation analysis, autoradiography, and gravimetry of uranium oxide after calcination. The resistance against static and dynamic leachings was also estimated. [less ▲]

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See detail3-Bromophenyl 6-acetoxymethyl-2-oxo-2H-1-benzopyran-3-carboxylate inhibits cancer cell invasion in vitro and tumour growth in vivo
Kempen, I.; Papapostolou, D.; Thierry, N. et al

in British Journal of Cancer (2003), 88(7), 1111-1118

In search for new anticancer agents, we have evaluated the antiinvasive and antimigrative properties of recently developed synthetic coumarin derivatives among which two compounds revealed important ... [more ▼]

In search for new anticancer agents, we have evaluated the antiinvasive and antimigrative properties of recently developed synthetic coumarin derivatives among which two compounds revealed important activity: 3-chlorophenyl 6-acetoxymethyl-2-oxo-2H-1-benzopyran-3-carboxylate and 3-bromophenyl 6-acetoxymethyl-2-oxo-2H-1-benzopyran-3-carboxylate, Both drugs were able to inhibit cell invasion markedly in a Boyden chamber assay, the bromo derivative being more potent than the reference matrix metalloprotease (MMP) inhibitor GI 129471. In vivo, tumour growth was reduced when nude mice grafted with HT 1080 or MDA-MB231 cells were treated i.p. 3 days week(-1) with the bromo coumarin derivative. These effects were not associated with the inhibition of urokinase, plasmin, MMP-2 or MMP-9. The mechanism of action of the drugs remains to be elucidated. However, these two coumarin derivatives may serve as new lead compounds of an original class of antitumour agents. [less ▲]

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See detailCritical thickness for ferroelectricity in perovskite ultrathin films
Junquera, J.; Ghosez, Philippe ULiege

in Nature (2003), 422(6931), 506-509

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See detailEffect of stocking density on the dynamics of cannibalism in sibling larvae of Perca fluviatilis under controlled conditions
Baras, E.; Kestemont, P.; Mélard, Charles ULiege

in Aquaculture (2003), 219(1-4), 241-255

The effect of stocking density (10, 31.6 and 100 larvae 1(-1); three replicates per treatment) on the day-by-day dynamics of survival, growth and cannibalism was examined in sibling perch larvae reared ... [more ▼]

The effect of stocking density (10, 31.6 and 100 larvae 1(-1); three replicates per treatment) on the day-by-day dynamics of survival, growth and cannibalism was examined in sibling perch larvae reared from eyed-egg stage in 100-1 cages (16L:8D, 20.0 +/- 0.5 degreesC, O-2 greater than or equal to 6.0 mg 1(-1); feeding in excess with live Artemia nauplii during the photophase) during the first 3 weeks of exogenous feeding. Larvae unable to achieve the transition to exogenous feeding died in between 7 and I I days post-hatch. Later, mortality from causes other than cannibalism never exceeded 1% day(-1). Cannibalism did not start before days 10 - 11 and first consisted in the incomplete ingestion of prey attacked tail first, exclusively. This type of cannibalism never caused losses higher than 2.0% of the initial stock, and ceased after days 16-18. From days 12-14 onwards, differential growth was apparent, and cannibals turned to complete cannibalism of small prey ingested head first, which caused greater losses (28-53% of the stock). Increasing the stocking density did not compromise growth and decreased the overall impact of cannibalism through several complementary mechanisms: (i) a postponed emergence of cannibalism, (ii) a lower proportion of cannibals in the population, and (iii) probably a lower rate of cannibalism per capita as predation was complicated and less directed at high stocking density. (C) 2003 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailDetection and clinical usefulness of a biphasic response during exercise echocardiography early after myocardial infarction
Lancellotti, Patrizio ULiege; Hoffer, E. P.; Pierard, L. A.

in Journal of the American College of Cardiology (2003), 41(7), 1142-1147

OBJECTIVES The aim of this study was to determine the accuracy of exercise echocardiography (EE) for detecting infarct-related artery (IRA) stenosis and predicting functional recovery early after acute ... [more ▼]

OBJECTIVES The aim of this study was to determine the accuracy of exercise echocardiography (EE) for detecting infarct-related artery (IRA) stenosis and predicting functional recovery early after acute myocardial infarction (AMI). BACKGROUND Dobutamine stress echocardiography is widely used for identifying jeopardized myocardium. The clinical usefulness of a biphasic response detected during EE has never been investigated. METHODS A total of 114 consecutive patients with a first AMI and greater than or equal to2 dyssynergic segments in the infarct-related territory underwent semi-supine continuous EE 6 +/- 2 days after AMI. Quantitative coronary angiography was performed in all patients after EE. A follow-up echocardiogram was obtained one month later. RESULTS Ninety-seven patients had significant (greater than or equal to50%) IRA stenosis, and 26 had multivessel disease. Residual ischemia was identified in 77 patients (biphasic response in 62 and worsening response in 15). The sensitivity and specificity of ischemia during EE for predicting IRA stenosis were 75% and 76%, respectively. The sensitivity of a biphasic response was higher than the sensitivity of a worsening response (61% vs. 14%, p < 0.0001). Wall motion abnormalities induced in other vascular territories were specific (97%) and moderately sensitive (62%) for the detection of multivessel disease. Functional recovery was observed in 75 patients. Two independent variables predicted contractile recovery: contractile reserve during EE (p < 0.0001) and elective angioplasty of the IRA (p = 0.002). A biphasic response, but not sustained improvement, predicted reversible dysfunction (73% vs. 9%, p < 0.0001). CONCLUSIONS A biphasic response can be detected during exercise. Exercise echocardiography is an accurate tool for detecting IRA stenosis and predicting functional improvement early after AMI. [less ▲]

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See detailSpectroscopic mode identification for the slowly pulsating B star HD 147394
Briquet, Maryline ULiege; Aerts, C.; Mathias, P. et al

in Astronomy and Astrophysics (2003), 401

We present ground-based high-resolution high S/N spectra of the slowly pulsating B star HD 147394. Two frequencies are clearly found in the moments of the Si II 4128-4130 doublet: f[SUB]1[/SUB]=0.8008 c d ... [more ▼]

We present ground-based high-resolution high S/N spectra of the slowly pulsating B star HD 147394. Two frequencies are clearly found in the moments of the Si II 4128-4130 doublet: f[SUB]1[/SUB]=0.8008 c d[SUP]-1[/SUP] and f[SUB]2[/SUB]=0.7813 c d[SUP]-1[/SUP]. The frequency f[SUB]1[/SUB] is also found in the HIPPARCOS data. A third frequency is also present in our spectroscopic data: f[SUB]3[/SUB]=0.7175 c d[SUP]-1[/SUP] or its aliases due to the time sampling. A mode identification is performed by means of a new version of the moment method optimized for multiperiodic signals. It points towards several equivalent solutions of non-axisymmetric l <=3 for all three modes. We have too limited information to perform seismic modelling at this stage, but we do show that the different possibilities for the mode identifications are compatible with pulsational models for SPBs. Based on observations obtained with the spectrograph Aurélie attached to the 1.52-m telescope of the Observatoire de Haute-Provence, France. [less ▲]

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See detailPregnancy and the immune system: between tolerance and rejection
Thellin, Olivier ULiege; Heinen, Ernst ULiege

in Toxicology (2003), 185(3), 179-184

Interactions between the conceptus and the mother are bi-directional: the feto-placental tissues need nutrition and a suitable environment in homeostatic condition whereas the mother influenced by the ... [more ▼]

Interactions between the conceptus and the mother are bi-directional: the feto-placental tissues need nutrition and a suitable environment in homeostatic condition whereas the mother influenced by the placental factors adapts her metabolism and immune system. Many different mechanisms acting locally or at distance ensure tolerance of the semi-allogeneic graft by the maternal natural and adaptive immune defences. In front of this tolerance, mechanisms exist ensuring rejection of the conceptus by the mother (spontaneous abortion) through rupture of one or more tolerance mechanisms, notably in stress situations endangering the mother. Thus outcome of a pregnancy is dependent on efficiently working tolerance mechanisms, and rupture of such mechanisms can lead to rejection. The balance of influence leading either to tolerance or rejection is under control of internal (maternal and fetal) and external (environmental) factors. Rejection, if triggered, mainly occurs through immune-induced inflammation, tissue degradation and coagulation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailAn X-ray investigation of the NGC 346 field in the Small Magellanic Cloud. II. The field population
Nazé, Yaël ULiege; Hartwell, J. M.; Stevens, I. R. et al

in Astrophysical Journal (2003), 586(2, Part 1), 983-995

We present results from a Chandra observation of the NGC 346 cluster, which is the ionizing source of N66, the most luminous H II region and the largest star formation region in the SMC. In the first part ... [more ▼]

We present results from a Chandra observation of the NGC 346 cluster, which is the ionizing source of N66, the most luminous H II region and the largest star formation region in the SMC. In the first part of this investigation, we have analyzed the X-ray properties of the cluster itself and the remarkable star HD 5980, but the field contains additional objects of interest. In total, 75 X-ray point sources were detected in the Chandra observation: this is 5 times the number of sources detected by previous X-ray surveys. We investigate here their characteristics in detail. Because of high foreground absorption, the sources possess rather high hardness ratios. Their cumulative luminosity function appears generally steeper than that for the rest of the SMC at higher luminosities. Their absorption columns suggest that most of the sources belong to NGC 346. Using Digitized Sky Survey data and new UBVRI imaging with the ESO 2.2 m telescope, we also discovered possible counterparts for 32 of these X-ray sources and estimated a B spectral type for a large number of these counterparts. This tends to suggest that most of the X-ray sources in the field are in fact X-ray binaries. Finally, some objects show X-ray and/or optical variability, with a need for further monitoring. [less ▲]

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See detailLifetimes, oscillator strengths and Lande factors in doubly ionized europium (Eu III)
Quinet, Pascal ULiege; Biémont, Emile ULiege

in Monthly Notices of the Royal Astronomical Society (2003), 340(2), 463-467

A set of transition probabilities has been calculated for Eu iii transitions of astrophysical interest particularly for the study of chemically peculiar stars. They were obtained taking configuration ... [more ▼]

A set of transition probabilities has been calculated for Eu iii transitions of astrophysical interest particularly for the study of chemically peculiar stars. They were obtained taking configuration interaction and core-polarization effects into account. The accuracy of the new scale of oscillator strengths, which differs substantially from previous results, has been assessed through comparisons with recent time-resolved laser-induced fluorescence measurements of radiative lifetimes. [less ▲]

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See detailWave packet dynamics along bifurcating reaction paths
Lasorne, Benjamin; Dive, Georges ULiege; Lauvergnat, David et al

in Journal of Chemical Physics (2003), 118(13), 5831-5840

The problem of bifurcating reaction paths is revisited by wave packet (WP) dynamics. The pitchfork model connecting five stationary points-a reactive, two transition structures and two enantiomeric ... [more ▼]

The problem of bifurcating reaction paths is revisited by wave packet (WP) dynamics. The pitchfork model connecting five stationary points-a reactive, two transition structures and two enantiomeric products-is characterized by a Valley Ridge inflection point (VRI) where WP could leave the standard intrinsic reaction path. We question the role of such a VRI point to determine whether the mechanism is sequential or concerted. WP simulations on two-dimensional minimum energy surfaces are carried out in the benchmark case of the methoxy radical isomerization H3CO-->H2COH. The ab initio potential energy surface (PES) is fitted to an analytical model which is bent to analyze the incidence of geometrical parameters on the WP behavior. For each of these generated PES, the WP width in the entrance valley is the main factor which conditions the behavior on the unstable ridge. The WP evolution is also analyzed in terms of nonadiabatic transitions among adiabatic channels along the reaction coordinate. Finally, the location of VRI points according to an invariant definition is discussed. (C) 2003 American Institute of Physics. [less ▲]

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See detailA new seismic analysis of Alpha Centauri
Thoul, Anne ULiege; Scuflaire, Richard ULiege; Noels-Grötsch, Arlette ULiege et al

in Astronomy and Astrophysics (2003), 402(1), 293-297

Models of alpha Cen A & B have been computed using the masses determined by Pourbaix et al. (\cite{Po2002}) and the data derived from the spectroscopic analysis of Neuforge & Magain (\cite{Ne1997}). The ... [more ▼]

Models of alpha Cen A & B have been computed using the masses determined by Pourbaix et al. (\cite{Po2002}) and the data derived from the spectroscopic analysis of Neuforge & Magain (\cite{Ne1997}). The seismological data obtained by Bouchy & Carrier (\cite{Bo2001}, \cite{Bo2002}) do help improve our knowledge of the evolutionary status of the system. All the constraints are satisfied with a model which gives an age of about 6 Gyr for the binary. [less ▲]

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See detailLate Quaternary clay mineral record in Central Lake Baikal (Academician Ridge, Siberia)
Fagel, Nathalie ULiege; Boski, T.; Likhoshway, L. et al

in Palaeogeography, Palaeoclimatology, Palaeoecology (2003), 193(1), 159-179

We investigated the mineralogical composition of two cores recovered on the Academician Ridge (Central Lake Baikal, Siberia). Sedimentological features show that the cores are unaffected by turbidity ... [more ▼]

We investigated the mineralogical composition of two cores recovered on the Academician Ridge (Central Lake Baikal, Siberia). Sedimentological features show that the cores are unaffected by turbidity currents. However, hemipelagic deposition is not continuous, but intermittently disturbed by syn- or post-sediment reworking (e.g., bioturbation, slumps, faulting). Such modes of deposition are consistent with the complex uplift history of the ridge. Bulk mineralogy suggests that terrigenous sediment supplies are constant through glacial/interglacial stages, and diluted by diatom-rich intervals related to warmer interglacial stages. The core stratigraphy is based on the correlation of the diatom zonation and opal abundance with the marine oxygen isotope reference curve SPECMAP. The similar to 8-m cores partly recover the last four interglacial/glacial cycles, i.e., since oxygen isotope stage 8. We test the use of clay minerals as a proxy for paleoclimatic reconstruction. The clays are more weathered during the diatom-rich intervals in agreement with warmer climate conditions. However, the mean clay composition does not change significantly through glacial/interglacial stages. This observation implies that, in the Academician Ridge sediments, a simple smectite/illite ratio (S/I) does not alone provide a reliable indicator of climatic variation. It reflects the complex clay assemblages, especially the smectite group, delivered to Central Lake Baikal. Smectites include primarily illite-smectite mixed layers, made of a mixture of montmorillonite and beidellite. According to their behavior after cation saturation, the illite-smectite mixed layers are primarily transformed smectites, with some neoformed smectites intermittently observed. In addition, Al-smectites occur in minor proportions. We conclude that the S/I ratio has a climatic significance only if it evolves in parallel with the weathering stage of the clays and is confirmed by a change in the composition of the smectites. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailVLT observations of the highly ionized nebula around Brey2
Nazé, Yaël ULiege; Rauw, Grégor ULiege; Manfroid, Jean ULiege et al

in Astronomy and Astrophysics (2003), 401(2), 13-16

We present the first high resolution He II lambda4686 images of the high excitation nebula around the WR star Brey2 in the LMC. This nebula presents a striking morphology: a small arc-like feature some 3 ... [more ▼]

We present the first high resolution He II lambda4686 images of the high excitation nebula around the WR star Brey2 in the LMC. This nebula presents a striking morphology: a small arc-like feature some 3.6 pc in radius is particularly prominent in the He II lambda4686 line. We further discover a previously unknown faint He II emission that extends over an area of 22 x 17 pc(2). An even fainter He II emission is apparently associated with the interstellar bubble blown by the progenitor of Brey2. The total He II flux corresponds to an ionizing flux of 4 x 10(47) photons s(-1). Halpha, [O III], and He I lambda5876 images and long-slit spectra are also examined in this letter, enabling us to investigate the detailed physical properties at various locations of the nebula. [less ▲]

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See detailIs laparoscopy still the gold standard in infertility assessment? A comparison of fertiloscopy versus laparoscopy in infertility. Results of an international multicentre prospective trial: the 'FLY' (Fertiloscopy-LaparoscopY) study.
Watrelot, A.; NISOLLE, Michelle ULiege; Chelli, H. et al

in Human Reproduction (2003), 18(4), 834-9

BACKGROUND: The aim of this prospective multicentre study was to compare the two endoscopic techniques of laparoscopy and fertiloscopy in routine evaluation of the pelvis in infertile women. METHODS: A ... [more ▼]

BACKGROUND: The aim of this prospective multicentre study was to compare the two endoscopic techniques of laparoscopy and fertiloscopy in routine evaluation of the pelvis in infertile women. METHODS: A total of 92 women was selected in 14 University Hospitals to undergo fertiloscopy followed by transabdominal laparoscopy by a team of two surgeons in each hospital. RESULTS: A high degree of concordance was observed between these two techniques, in that if fertiloscopy did not detect any abnormalities, this was also confirmed by laparoscopy. Discordance was observed in similar numbers of cases: eight after laparoscopy and nine after fertiloscopy. The diagnostic index for fertiloscopy and laparoscopy was calculated; sensitivity (86 and 87% respectively) and negative predictive value (64 and 67% respectively) were similar. The kappa index was also calculated for each of the six structures/regions (right/left tube; right/left ovary; peritoneum of pouch of Douglas; posterior uterus), and concordance (0.78 to 0.91) was considered almost complete. CONCLUSIONS: These results confirm fertiloscopy as a minimally invasive safe procedure that may be considered as an alternative to diagnostic laparoscopy in the routine assessment of women without clinical or ultrasound evidence of pelvic disease. On the basis of the additional advantages of fertiloscopy, namely salpingoscopy or microsalpingoscopy, it is considered that fertiloscopy could replace laparoscopy as a routine procedure in such women. [less ▲]

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See detailSound-producing mechanisms and recordings in Carapini species (Teleostei, Pisces)
Parmentier, Eric ULiege; Vandewalle, Pierre ULiege; Lagardere, Jean-Pierre

in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology (2003), 189(4), 283-292

Carapus boraborensis, C. homei and Encheliophis gracilis are three species of Carapidae that display the ability to penetrate and reside in the holothurian Bohadschia argus. This study describes both the ... [more ▼]

Carapus boraborensis, C. homei and Encheliophis gracilis are three species of Carapidae that display the ability to penetrate and reside in the holothurian Bohadschia argus. This study describes both the particular morphology of the sound-producing structures and, for the first time, the sounds produced by each species. The study of the structures composing the sound-producing system seems to indicate that the action made by the primary sonic muscles (i.e. the pulling and releasing of the front of the swim bladder) might be responsible for the sound emissions of these three species by provoking a vibration of a thinner zone in front of the swim bladder (swimbladder fenestra). The sounds were only emitted and recorded when several individuals of the same species were inside the same sea cucumber. They were composed of serially repeated knocks and were heard as drum beats or drum rolls. Their specific differences were mainly defined as variations in the timing or grouping of the knocking sounds. The recordings of these sound productions demonstrate a vocal ability for the three species, linked with the presence of particular organs associated with sound production. Moreover, the ecological significance of the sounds and of the sound apparatus system is discussed. [less ▲]

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See detailDossier en souffrance
Frankignoulle, Pierre ULiege; Stevens, Barbara

in A+ : Architecture in Belgium (2003)

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See detailReexamination of the Branly effect
Dorbolo, Stéphane ULiege; Ausloos, Marcel ULiege; Vandewalle, Nicolas ULiege

in Physical Review E (2003), 67(4, Pt 1), 40302

The electrical resistance of a metallic granular packing has been recorded at room temperature. A nearby burster between which sparks are produced, induces a decrease in the resistance of the granular ... [more ▼]

The electrical resistance of a metallic granular packing has been recorded at room temperature. A nearby burster between which sparks are produced, induces a decrease in the resistance of the granular packing as described in the works of Branly. Our measurements emphasize that the decrease is continuous and the resistance variations behave like a stretched exponential law due to the creation of new electrical paths as in nucleation-growth soldering processes. This behavior has been identified to be a diffusionlike process. [less ▲]

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See detailMice without uPA, tPA, or plasminogen genes are resistant to experimental choroidal neovascularization
Rakic, Jean-Marie ULiege; Lambert, Vincent ULiege; Munaut, Carine ULiege et al

in Investigative Ophthalmology & Visual Science (2003), 44(4), 1732-1739

PURPOSE. To evaluate the presence and potential involvement of members of the plasminogen/plasminogen activator (Plg/PA) system in the exudative form of age-related macular degeneration (AMD). METHODS ... [more ▼]

PURPOSE. To evaluate the presence and potential involvement of members of the plasminogen/plasminogen activator (Plg/PA) system in the exudative form of age-related macular degeneration (AMD). METHODS. The expression of PA members mRNA was evaluated in human and experimental choroidal neovascularization (CNV) by RT-PCR. The presence and activity of PA was studied by immunofluorescence and in situ zymography. The influence of endogenous plasminogen (Plg), urokinase (uPA), tissue type plasminogen activator (tPA), and uPA receptor (uPAR) was explored in single-gene-deficient mice in a model of laser-induced CNV. RESULTS. Members of the Plg/PA system were present both in human and murine CNV. The absence of Pig, uPA, or tPA significantly decreased the development of experimental CNV compared with wild-type or uPAR-deficient mice. This effect could be attributable, partly to a modulation of matrix metalloproteinase activity, but also to an accumulation of fibrinogen-fibrin in the laser-induced wounds. CONCLUSIONS. Together with previous work done by the authors, this study indicates that choroidal neovascularization is extremely sensitive to the modulation of Plg/PA system activity. This may provide a new strategy for the treatment of exudative AMD. [less ▲]

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See detailMarrow stromal cell recovery after radiation-induced aplasia in mice
Almohamad, Khaled; Thiry, Albert ULiege; Hubin, Frédérique et al

in International Journal of Radiation Biology (2003), 79(4), 259-67

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See detailAlimentation et cancer
Castronovo, Vincenzo ULiege

in Revue Médicale de Liège (2003), 58(4), 231-9

The role of a qualitatively and quantitatively altered nutrition for the development of cancer in human is largely recognized. While the human life expectancy is continuously expanding and the World ... [more ▼]

The role of a qualitatively and quantitatively altered nutrition for the development of cancer in human is largely recognized. While the human life expectancy is continuously expanding and the World Health Organization is predicting a dramatic rise in the number of patients that will get cancer and die from it in the next decades, it is useful to attempt to understand the real impact of nutrition at the level of the oncogenesis mechanisms. Oxidative stress, methylation deficit and imbalance in the ratio of omega-3 and omega-6 fatty acids represents situations directly linked to nutrition and which contribute to an increased risk for cancer development. The understanding of the mechanisms by which nutrition affects these processes should better stimulate health professionals to consider with more attention what their patients are eating. [less ▲]

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See detailPolymerization-filled composites and nanocomposites by coordination catalysis
Dubois, Philippe ULiege; Alexandre, Michaël ULiege; Jérôme, Robert ULiege

in Macromolecular Symposia (2003), 194

Polyolefinic microcomposites and layered silicate nanocomposites were prepared by the in-situ polymerization of ethylene (and alpha-olefin) via the so-called polymerization-filling technique. Various ... [more ▼]

Polyolefinic microcomposites and layered silicate nanocomposites were prepared by the in-situ polymerization of ethylene (and alpha-olefin) via the so-called polymerization-filling technique. Various tillers with either basic, acidic, metallic or carbonaceous surface, as well as non-modified clays such as montmorillonite and hectorite, were first treated by trimethylaluminum-depleted methylaluminoxane before being contacted by a titanium or zirconium-based metallocene catalyst. The (nano)composite was formed by addition and polymerization of ethylene (and alpha-olefin). This technique makes possible the complete encapsulation of every filler particle within the (co)polyolefinic matrix ranging from thermoplastics to elastomers. The so-obtained (nano)composites, often coined as "homogeneous" (nano)composites, exhibit improved mechanical properties; as compared to more conventional melt blends for a same filler content. [less ▲]

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See detailEnhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation.
Maquoi, Erik ULiege; Demeulemeester, Diego; Vörös, Gabor et al

in Thrombosis and Haemostasis (2003), 89(4), 696-704

To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat ... [more ▼]

To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat diet (HFD) for 15 weeks. MMP-3(-/-) mice were hyperphagic and gained more weight than the MMP-3(+/+) mice. At the time of sacrifice, the body weight of the MMP-3(-/-) mice was significantly higher than that of the MMP-3(+/+) mice, as was the weight of the isolated subcutaneous (SC) and gonadal (GON) fat deposits. Significant adipocyte hypertrophy was observed in the GON but not in the SC adipose tissue of MMP-3(-/-) mice. Fasting plasma glucose and cholesterol levels were comparable in both genotypes, whereas triglyceride levels were significantly lower in MMP-3(-/-) mice. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density and larger total stained area in the GON adipose tissues of MMP-3(-/-) mice. Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis. [less ▲]

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See detailPrevention primaire et secondaire du cancer colorectal
Polus, Marc ULiege; Piront, Patricia ULiege; Jerusalem, Guy ULiege et al

in Revue Médicale de Liège (2003), 58(4), 247-53

Colorectal cancer is really a public health problem. The authors review the literature about the environmental factors leading to colorectal cancer. Chemoprevention of colorectal cancer is also discussed ... [more ▼]

Colorectal cancer is really a public health problem. The authors review the literature about the environmental factors leading to colorectal cancer. Chemoprevention of colorectal cancer is also discussed, particularly by aspirin and non steroidal anti-inflammatory drugs. Development of specific cyclooxygenase-2 inhibitors constitutes a promising research's field. Secondary prevention by coloscopy and polypectomy must lead to a lower rate of colorectal cancer disease and improvement of mortality. [less ▲]

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See detailAnalysis of the importance of the metallo-beta-lactamase active site loop in substrate binding and catalysis
Moali, C.; Anne, C.; Lamotte-Brasseur, J. et al

in Chemistry & Biology (2003), 10(4), 319-329

The role of the mobile loop comprising residues 60-66 in metallo-beta-lactamases has been studied by site-directed mutagenesis, determination of kinetic parameters for six substrates and two inhibitors ... [more ▼]

The role of the mobile loop comprising residues 60-66 in metallo-beta-lactamases has been studied by site-directed mutagenesis, determination of kinetic parameters for six substrates and two inhibitors, pre-steady-state characterization of the interaction with chromogenic nitrocefin, and molecular modeling. The W64A mutation was performed in IMP-1 and BcII (after replacement of the Bcll 60-66 peptide by that of IMP-1) and always resulted in increased K-i and K. and decreased k(cat)/K-m values, an effect reinforced by complete deletion of the loop. k(cat) values were, by contrast, much more diversely affected, indicating that the loop does not systematically favor the best relative positioning of substrate and enzyme catalytic groups. The hydrophobic nature of the ligand is also crucial to strong interactions with the loop, since imipenem was almost insensitive to loop modifications. [less ▲]

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See detailL'hypertension artérielle essentielle peut-elle être prevenue?
Krzesinski, Jean-Marie ULiege; Saint-Remy, Annie ULiege

in Revue Médicale de Liège (2003), 58(4), 198-205

Prevalence of arterial hypertension is growing, in particular due to population ageing. Hypertension is a major risk factor for cardiovascular morbidity and mortality. Prevention is conceivable on a ... [more ▼]

Prevalence of arterial hypertension is growing, in particular due to population ageing. Hypertension is a major risk factor for cardiovascular morbidity and mortality. Prevention is conceivable on a theoretical basis by reduction or, if possible, prevention of excess weight, diet modifications, increased physical activity among other means. To be efficient, prevention should include an early detection of patients at risk and a long term assessment of efficacy requiring many efforts from both patients and physicians. [less ▲]

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