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See detailSteroid receptor coactivator SRC-1 exhibits high expression in steroid-sensitive brain areas regulating reproductive behaviors in the quail brain.
Charlier, Thierry ULg; Lakaye, Bernard ULg; Ball, Gregory F et al

in Neuroendocrinology (2002), 76(5), 297-315

The steroid receptor coactivator SRC-1 modulates ligand-dependent transactivation of several nuclear receptors, including the receptors for sex steroid hormones. Reducing the expression of SRC-1 by ... [more ▼]

The steroid receptor coactivator SRC-1 modulates ligand-dependent transactivation of several nuclear receptors, including the receptors for sex steroid hormones. Reducing the expression of SRC-1 by injection of specific antisense oligonucleotides markedly inhibits the effects of estrogens of the sexual differentiation of brain and behavior in rats and inhibits the activation of female sexual behavior in adult female rats. SRC-1 thus appears to be involved in both the development and activation of sexual behavior. In the Japanese quail brain, we amplified by RT-PCR a 3,411-bp fragment extending from the HLH domain to the activating domain-2 of the protein. The quail SRC-1 is closely related to the mammalian (m) SRC-1 and contains a high proportion of GC nucleotides (62.5%). Its amino acid sequence presents 70% identity with mammalian SRC-1 and contains the three conserved LXXLL boxes involved in the interaction with nuclear receptors. In both males and females, RT-PCR demonstrates a similarly high level of expression in the telencephalon, diencephalon, optic lobes, brain stem, spinal cord, pituitary, liver, kidney, adrenal gland, heart, lung, gonads and gonoducts. Males express significantly higher levels of SRC-1 in the preoptic area-hypothalamus than females. In both sexes, lower levels of expression are observed in the cerebellum and muscles. In situ hybridization utilizing a mixture of four digoxigenin-labeled oligonucleotides confirms at the cellular level the widespread distribution of SRC-1 mRNA in the brain and a particularly dense expression in steroid-sensitive areas that play a key role in the control of male sexual behavior. These data confirm the presence and describe for the first time the SRC-1 distribution in the brain of an avian species. They confirm its broad, nearly ubiquitous, distribution in the entire body including the brain as could be expected for a coactivator that regulates to the action of many nuclear receptors. However this distribution is heterogeneous in the brain and sexually differentiated in at least some areas. The very dense expression of SRC-1 in limbic and mesencephalic nuclei that are associated with the control of male sexual behavior is consistent with the notion that this coactivator plays a significant role in the activation of this behavior. [less ▲]

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See detailSociété civile et transition. Un regard sur l'évolution du projet de constitution d'une société civile en Roumanie postcommuniste
Pirotte, Gautier ULg

in Transitions : Ex-Revue des Pays de L'est (2002), XLIII(1),

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See detailMigration between States and Markets (Conference Report)
Martiniello, Marco ULg

in International Migration Review (2002), 36(2), 593-596

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See detailLes Parents maltraités
Malchair, Alain ULg

in Neurone (2002), 7(3), 83-86

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See detailContinuity preserving modified maximum cross-correlation technique
Zavialov, Peter O.; Grigorieva, Julia V.; Moller, Osmar O. et al

in Journal of Geophysical Research. Oceans (2002), 107(C10, SEP-OCT),

The maximum cross-correlation (MCC) method reconstructs the surface advective velocity fields from the displacements of spatial patterns in pairs of sequential satellite (normally infrared) images ... [more ▼]

The maximum cross-correlation (MCC) method reconstructs the surface advective velocity fields from the displacements of spatial patterns in pairs of sequential satellite (normally infrared) images. However, the performance of the conventional MCC method is not always satisfactory. One of the main reasons for this is the fact that the method can correctly estimate only the velocity component parallel to the gradient of the property depicted in the images, while any small displacement perpendicular to the gradient (i.e., directed along the isolines) essentially maps the spatial pattern onto itself and therefore can not be detected using the conventional MCC technique. In the present work we propose a modification of the MCC method that allows circumventing this basic deficiency and improving the performance of the MCC technique. In this approach, the "cross-isoline'' components of the velocity field are obtained as in the conventional MCC scheme; however, the "along-isoline'' components derived from the MCC are disregarded as unreliable. Instead, the "true'' along-isoline components are then reconstructed from the given cross-isoline velocity field based on the continuity requirement and on the condition of no normal flow at solid boundaries. This inverse problem is solved by constructing the two-dimensional stream function in the curvilinear coordinate frame associated with the image isolines. The method is illustrated using AVHRR images from the southwestern Atlantic Ocean and the Black Sea. The results are compared with some direct drifter and current meter measurements and geostrophic estimates. [less ▲]

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See detailL'engoument pour la "Cène" de Léonard de Vinci à la cour de France, sous les règnes de Louis XII et de François Ier
Fagnart, Laure ULg

in Bulletin des amis du château et des musées de Blois (2002), 33

Cette contribution présente les copies de la "Cène" de Léonard de Vinci qui ont été réalisées à la demande de Français dans la première moitié du XVIe siècle. Elle s’attache à comprendre comment et ... [more ▼]

Cette contribution présente les copies de la "Cène" de Léonard de Vinci qui ont été réalisées à la demande de Français dans la première moitié du XVIe siècle. Elle s’attache à comprendre comment et pourquoi autant de Français, souvent en poste dans l’administration française à Milan, ont commandé des copies du chef-d’œuvre du maître italien, définissant de ce fait les mécanismes de l’engouement pour la peinture murale à la cour de France, sous les règnes de Louis XII et de François Ier. [less ▲]

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See detailContinuité de la délinquance entre l’adolescence et l’âge adulte
Born, Michel ULg

in Criminologie (2002), 35(1), 53-67

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See detailChanges in the development of the winter-spring phytoplankton bloom in the Bay of Calvi (NW Mediterranean) over the last two decades: a response to changing climate?
Goffart, Anne ULg; Hecq, Jean-Henri ULg; Legendre, Louis

in Marine Ecology. Progress Series (2002), 236

The development of the winter-spring phytoplankton bloom was investigated in the Bay of Calvi (Corsica, Ligurian Sea, northwestern Mediterranean) in 1979, 1986, 1988, 1997 and 1998. A drastic reduction of ... [more ▼]

The development of the winter-spring phytoplankton bloom was investigated in the Bay of Calvi (Corsica, Ligurian Sea, northwestern Mediterranean) in 1979, 1986, 1988, 1997 and 1998. A drastic reduction of phytoplankton biomass was evidenced over the last 2 decades, in relation to long-term changes in climatic and environmental conditions. Between 1979 and 1998, the monthly averaged chlorophyll a concentrations at 1 m decreased by about 80% during February, March and April. Simultaneously, major changes to hydrodynamic conditions include warmer water, overall decrease of salinity at 10 m depth, longer periods of bright sunshine and lower wind stress, The changes in environmental conditions were large enough to affect the vertical stability of the water column during the winter-spring period and to reduce nutrient replenishment of the surface layer prior to the usual period of phytoplankton growth. Until 1986, the main factor driving nutrient replenishment was the winter upward mixing of nutrient-rich deep waters, while the progressive reduction of mixing from 1988 induced nutrient limitation of surface waters in the last decade. The following hypotheses on changes in the development of the winter-spring phytoplankton bloom are made: (1) Until 1986, phytoplankton peaks took place in relatively high-nutrient waters and were diatom-dominated. (2) Between 1986 and 1988, decreasing Si availability led to Si limitation which caused a reduction in diatom abundance. This resulted in the disappearance of the diatom-dominated pulses and in lower phytoplankton biomass and was accompanied by a shift toward non-siliceous phytoplankton. (3) In 1988, 1997 and 1998, decreasing nitrate availability led to nitrate limitation, thus explaining the progressive reduction in non-siliceous phytoplankton biomass. Other, associated changes in benthos assemblages and ichthyofauna are documented. The conclusions from the Bay of Calvi are extended to the whole western Corsican coast. This confirms that the Mediterranean reacts rapidly to external perturbations, which are driven by climate change in that particular area. [less ▲]

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See detailSynthesis, cytotoxicity, and antiplasmodial and antitrypanosomal activity of new neocryptolepine derivatives.
Jonckers, Tim H M; van Miert, Sabine; Cimanga, Kanyanga et al

in Journal of Medicinal Chemistry (2002), 45(16), 3497-508

On the basis of the original lead neocryptolepine or 5-methyl-5H-indolo[2,3-b]quinoline, an alkaloid from Cryptolepis sanguinolenta, derivatives were prepared using a biradical cyclization methodology ... [more ▼]

On the basis of the original lead neocryptolepine or 5-methyl-5H-indolo[2,3-b]quinoline, an alkaloid from Cryptolepis sanguinolenta, derivatives were prepared using a biradical cyclization methodology. Starting from easily accessible educts, this approach allowed the synthesis of hitherto unknown compounds with a varied substitution pattern. As a result of steric hindrance, preferential formation of the 3-substituted isomers over the 1-substituted isomers was observed when cyclizing N-(3-substituted-phenyl)-N'-[2-(2-trimethylsilylethynyl)phenyl]carbodiimides. All compounds were evaluated for their activity against chloroquine-sensitive as well as chloroquine-resistant Plasmodium falciparum strains, for their activity against Trypanosoma brucei and T. cruzi, and for their cytotoxicity on human MRC-5 cells. Mechanisms of action were investigated by testing heme complexation using ESI-MS, inhibition of beta-hematin formation, DNA interactions (DNA-methyl green assay and linear dichroism), and inhibition of human topoisomerase II. Neocryptolepine derivatives with a higher antiplasmodial activity and a lower cytotoxicity than the original lead have been obtained. This selective antiplasmodial activity was associated with inhibition of beta-hematin formation. 2-Bromoneocryptolepine was the most selective compound with an IC(50) value against chloroquine-resistant P. falciparum of 4.0 microM in the absence of cytotoxicity (IC(50) > 32 microM). Although cryptolepine, a known lead for antimalarials also originally isolated from Cryptolepis sanguinolenta, was more active (IC(50) = 2.0 microM), 2-bromoneocryptolepine showed a low affinity for DNA and no inhibition of human topoisomerase II, in contrast to cryptolepine. Although some neocryptolepine derivatives showed a higher antiplasmodial activity than 2-bromocryptolepine, these compounds also showed a higher affinity for DNA and/or a more pronounced cytotoxicity. Therefore, 2-bromoneocryptolepine is considered as the most promising lead from the present work for new antimalarial agents. In addition, 2-bromo-, 2-nitro-, and 2-methoxy-9-cyanoneocryptolepine exhibited antitrypanosomal activity in the micromolar range in the absence of obvious cytotoxicity. [less ▲]

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See detailLa forêt et la protection du sol dans le cadre d'aménagement forestiers intégrés
Huart, O.; Adam, V.; Quevy, B. et al

in Fiche technique (2002), (14),

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See detailDistribution and air-water exchange of carbon dioxide in the Scheldt plume off the Belgian coast
Borges, Alberto ULg; Frankignoulle, Michel

in Biogeochemistry (2002), 59((1-2)), 41-67

In the present paper we report partial pressure of CO2 (pCO2) data obtained off the Belgian coast during 24 cruises. The temporal and spatial resolution of this data set allows us to discuss ... [more ▼]

In the present paper we report partial pressure of CO2 (pCO2) data obtained off the Belgian coast during 24 cruises. The temporal and spatial resolution of this data set allows us to discuss satisfactorily seasonal and inter-annual variability of pCO2 in the study area. The dynamics of pCO2 are described using two approaches: fixed reference stations and area survey cruises. The air-water fluxes of CO2 in the Scheldt estuarine plume and in the outerplume region are estimated quantitatively, showing that these areas correspond respectively to a net annual source and sink of atmospheric CO2. The annually integrated air-water fluxes for the Scheldt estuarine plume range between +1.1 and +1.9 mol m−2 year−1 as a function of the formulation of the exchange coefficient of CO2. The annual net emission of CO2 from the estuarine plume to the atmosphere is estimated to be between +2.3 to +4.0 Gmol year−1 which represents 17 to 29% of the estimate reported in the literature for the Scheldt inner estuary. [less ▲]

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See detailCytotoxic aporphine alkaloids from Cassytha filiformis.
Stevigny, C.; Block, S.; De Pauw-Gillet, Marie-Claire ULg et al

in Planta Medica (2002), 68(11), 1042-4

Purification of a cytotoxic crude alkaloid extract of Cassytha filiformis led to the isolation of four known aporphine alkaloids: neolitsine, dicentrine, cassythine (= cassyfiline) and actinodaphnine ... [more ▼]

Purification of a cytotoxic crude alkaloid extract of Cassytha filiformis led to the isolation of four known aporphine alkaloids: neolitsine, dicentrine, cassythine (= cassyfiline) and actinodaphnine. Their structures were determined by analysis of spectroscopic data. All isolated alkaloids were tested for their cytotoxic activities on cancer and non-cancer cell lines in vitro. Neolitsine was the most active against HeLa and 3T3 cells (IC 50 :21.6 microM, and 21.4 microM, respectively). Cassythine and actinodaphnine showed the highest activity against Mel-5 (IC 50 : 24.3 microM and 25.7 microM, respectively) and HL-60 (IC 50 : 19.9 microM and 15.4 microM, respectively). This is the first report on the cytotoxic activity of C. filiformis extract and of neolitsine and cassythine. Furthermore, the complete NMR data of cassythine and actinodaphnine are given here for the first time. [less ▲]

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See detailWave packet propagation for constrained molecular systems. Spectroscopic applications to triatomic molecules
Lauvergnat, David; Justum, David; Desouter, Michèle ULg et al

in Journal of Molecular Structure : Theochem (2002), 577

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See detailElevated atmospheric CO2 in open top chambers increases net nitrification and potential denitrification
Carnol, Monique ULg; Hogenboom, L.; Jach, M. E. et al

in Global Change Biology (2002), 8

The control of soil nitrogen (N) availability under elevated atmospheric CO2 is central to predicting changes in ecosystem carbon (C) storage and primary productivity. The effects of elevated CO2 on ... [more ▼]

The control of soil nitrogen (N) availability under elevated atmospheric CO2 is central to predicting changes in ecosystem carbon (C) storage and primary productivity. The effects of elevated CO2 on belowground processes have so far attracted limited research and they are assumed to be controlled by indirect effects through changes in plant physiology and chemistry. In this study, we investigated the effects of a 4-year exposure to elevated CO2 (ambient + 400 mumol mol(-1) ) in open top chambers under Scots pine (Pinus sylvestris L) seedlings on soil microbial processes of nitrification and denitrification. Potential denitrification (DP) and potential N-2 O emissions were significantly higher in soils from the elevated CO2 treatment, probably regulated indirectly by the changes in soil conditions (increased pH, C availability and NO3 (-) production). Net N mineralization was mainly accounted for by nitrate production. Nitrate production was significantly larger for soil from the elevated CO2 treatment in the field when incubated in the laboratory under elevated CO2 (increase of 100%), but there was no effect when incubated under ambient CO2 . Net nitrate production of the soil originating from the ambient CO2 treatment in the field was not influenced by laboratory incubation conditions. These results indicate that a direct effect of elevated atmospheric CO2 on soil microbial processes might take place. We hypothesize that physiological adaptation or selection of nitrifiers could occur under elevated CO2 through higher soil CO2 concentrations. Alternatively, lower microbial NH4 assimilation under elevated CO2 might explain the higher net nitrification. We conclude that elevated atmospheric CO2 has a major direct effect on the soil microbial processes of nitrification and denitrification despite generally higher soil CO2 concentrations compared to atmospheric concentrations. [less ▲]

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See detailRecommendations for the design of clinical trials and the registration of drugs used in the treatment of asthma
Holgate, ST; Bousquet, J; Chung, KF et al

in International Journal of Pharmaceutical Medicine (2002), 16

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See detailCompte rendu des actes du colloque : Unanimité et diversité cisterciennes
Delfosse, Annick ULg

in Moyen Age (Le) (2002), 108(2), 380-382

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See detailInfiltrative lipoma in the foreleg of a horse
Olle, Edwin; Saunders, Jimmy; Desmecht, Daniel ULg

in Vlaams Diergeneeskundig Tijdschrift (2002), 71

This case report describes an infiltrative lipoma in a horse. A slow-growing mass, detected early afterbirth, was present at the caudal aspect of the foreleg (antebrachium) of a 2-year-old gelding ... [more ▼]

This case report describes an infiltrative lipoma in a horse. A slow-growing mass, detected early afterbirth, was present at the caudal aspect of the foreleg (antebrachium) of a 2-year-old gelding. Cytological fea-tures were that of a benign lipoma. Surgical resection was attempted but failed due to the infiltrative natureof the mass. Consequently, the horse was euthanized. Histopathology revealed an infiltrative lipoma. Thistumor is histologically benign but surgical resection is usually impossible due to its infiltrative nature. [less ▲]

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See detailNew 3-deoxyanthocyanidins from leaves of Arrabidaea chica
Devia, B.; Llabres, Gabriel ULg; Wouters, J. et al

in Phytochemical Analysis (2002), 13(2, Mar-Apr), 114-120

Two new 3-deoxyanthocyanidins, 6,7,3',4'-tetrahydroxy-5-methoxyflavylium and 6,7,4'-trihydroxy-5-methoxyflavylium, and the pigment carajurin, which has been previously identified, were isolated from dried ... [more ▼]

Two new 3-deoxyanthocyanidins, 6,7,3',4'-tetrahydroxy-5-methoxyflavylium and 6,7,4'-trihydroxy-5-methoxyflavylium, and the pigment carajurin, which has been previously identified, were isolated from dried leaves of Arrabidaea chica, a creeper native to the American tropics. The structures of the components were elucidated by H-1- and C-13-NMR spectroscopy and HPLC-MS, including X-ray crystallographic analysis for carajurin. Copyright (C) 2002 John Wiley Sons, Ltd. [less ▲]

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See detailGlobal democratic consensus on neuropathological disease criteria.
Achim, Cristian; Auer, Roland; Bergeron, Catherine et al

in Lancet Neurology (2002), 1(6), 340

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See detailMedical imaging and MRI in nail disorders: report of 119 cases and review of the literature
Richert, Bertrand ULg; Baghaie, Mahmoud

in Dermatologic Therapy (2002), 15

Radiographs remain the golden standard for exploration of the bony structures located beneath the nail plate, but they provide no information on the perionychium. Until a few years ago the nail apparatus ... [more ▼]

Radiographs remain the golden standard for exploration of the bony structures located beneath the nail plate, but they provide no information on the perionychium. Until a few years ago the nail apparatus was deprived of investigative medical imaging. Glomus tumor was the only condition that was explored using invasive techniques such as angiography or scintigraphy. High-frequency ultrasound rapidly came up against technical limits. High-resolution magnetic resonance imaging (MRI) offers a superior alternative in detection of distal lesions as well as their relationship with the adjacent structures. MRI provides an accurate analysis of the nail apparatus with detection of lesions as small as 1 mm. This noninvasive technique will allow us to better understand, diagnose, and treat pathologies of the distal phalanx. [less ▲]

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See detailPrévention et traitement des nausées et vomissements après chimiothérapie anticancéreuse
Brasseur, Edmond ULg; Silvestre, Rose-Marie; Jerusalem, Guy ULg et al

in Médecine et Hygiène (2002), 60

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See detailSensitive and high throughput metabolite assays for inorganic pyrophosphate, ADPGlc, nucleotide phosphates, and glycolytic intermediates based on a novel enzymic cycling system.
Gibon, Yves; Vigeolas, Hélène ULg; Tiessen, Axel et al

in Plant Journal (The) (2002), 30(2), 221-35

Metabolite assays are required to characterise how metabolism changes between genotypes during development and in response to environmental perturbations. They provide a springboard to identify important ... [more ▼]

Metabolite assays are required to characterise how metabolism changes between genotypes during development and in response to environmental perturbations. They provide a springboard to identify important regulatory sites and investigate the underlying mechanisms. Due to their small size, Arabidopsis seeds pose a technical challenge for such measurements. A set of assays based on a novel enzymic cycling system between glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate oxidase have been developed and optimised for use with growing Arabidopsis seeds. In combination with existing assays they provide a suite of high throughput, sensitive assays for the immediate precursors for starch (adenine diphosphate glucose) and lipid (acetyl coenzyme A, glycerol-3-phosphate) synthesis, as well as pyrophosphate, ATP, ADP and most of the glycolytic intermediates. A method is also presented to rapidly quench intact siliques, lyophilise them and then manually separate seeds for metabolite analysis. These techniques are used to investigate changes in overall seed metabolite levels during development and maturation, and in response to a stepwise decrease of the external oxygen concentration. [less ▲]

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See detailCompte rendu de R. GODDING, Prêtres en Gaule mérovingienne, Bruxelles, 2001
George, Philippe ULg

in Moyen Age (Le) (2002)

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See detailGenetic variation and evolution in the genus Apodemus (Muridae : Rodentia)
Filippucci, M. G.; Macholan, M.; Michaux, Johan ULg

in Biological Journal of the Linnean Society (2002), 75(3), 395-419

Genetic variation was studied using protein electrophoresis of 28-38 gene loci in 1347 specimens of Apodemus agrarius, A. peninsulac, A. flavicollis, A. sylvaticus, A. alpicola, A. uralensis, A. cf ... [more ▼]

Genetic variation was studied using protein electrophoresis of 28-38 gene loci in 1347 specimens of Apodemus agrarius, A. peninsulac, A. flavicollis, A. sylvaticus, A. alpicola, A. uralensis, A. cf. hyrcanicus, A. hermonensis, A. m. mystacinus and A. in. epimelas, representing 121 populations from Europe, the Middle East, and North Africa. Mean values of heterozygosity per locus for each species ranged from 0.02 to 0.04. Mean values of Nei's genetic distance (D) between the taxa ranged from 0.06 (between A. flavicollis and A. alpicola) to 1.34 (between A. uralensis and A. agrarius). The highest values of D were found between A. agrarius and other Apodemus species (0.62-1.34). These values correspond to those generally observed between genera in small mammals. Our data show that A. agrarius and A. peninsulae are sister species, well-differentiated from other taxa. High genetic distance between A. m. mystacinus and A. m. epimelas leads us to consider them distinct species and sister taxa to other Western Palaearctic species of the subgenus Sylvaemus. The data also suggest a recent separation of members of the latter group from a common ancestor, and subsequent rapid radiation, making it difficult to infer phylogenetic relationships. Some taxonomic implications of the results are discussed further. (C) 2002 The Linnean Society of London. [less ▲]

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See detailLes débuts du paléolithique supérieur dans le nord-ouest de l’Europe : le Lincombien-Ranisien-Jerzmanowicien (état de la question)
Flas, Damien ULg

in Anthropologica et Praehistorica : Bulletin de la Société Royale Belge d'Anthropologie et de Préhistoire (2002), 113

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See detailEchouage de mammifères marins : guide d'intervention et procédures d'autopsie
Jauniaux, Thierry ULg; Garcia-Hartmann, M.; Haelters, J. et al

in Annales de Médecine Vétérinaire (2002), 146(5, OCT-NOV), 261-276

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See detailLes Relations entre capacités lexicales et mémoire phonologique à court terme dans le syndrome de Down
Comblain, Annick ULg

in Orthophonie et Logopédie (2002), 1

Certaines études neuropsychologiques de patients cérébro-lésés suggèrent que la mémoire de travail contribue à l'apprentissage à long terme de structures phonologiques non-familières. Les données ... [more ▼]

Certaines études neuropsychologiques de patients cérébro-lésés suggèrent que la mémoire de travail contribue à l'apprentissage à long terme de structures phonologiques non-familières. Les données disponibles sur des enfants normaux indiquent que la mémoire phonologique à court terme influence l'acquisition de nouveaux mots de vocabulaire. Par ailleurs, on sait que les personnes trisomiques 21 ont une mémoire phonologique à court terme déficiente ainsi que des connaissances lexicales limitées. Dès lors, étudier le lien entre ces deux variables dans la trisomie 21 s'imposait. Nos résultats indiquent que chez ces sujets, tout comme chez les enfants normaux, le niveau de développement lexical semble étroitement lié à la mémoire phonologique à court terme et plus particulièrement aux capacités de répétition de non-mots. [less ▲]

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See detailThe Disulphide Mapping, Folding and Characterisation of Recombinant Ber e 1, an Allergenic Protein, and SFA8, Two Sulphur-rich 2 S Plant Albumins
Alcocer, Marcos; Murtagh, G. J.; Bailey, Kevin et al

in Journal of Molecular Biology (2002), 324

We have cloned and expressed genes encoding the allergenic brazil nut 2 S albumin (Ber e 1) and the sunflower albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris. We show that both proteins were ... [more ▼]

We have cloned and expressed genes encoding the allergenic brazil nut 2 S albumin (Ber e 1) and the sunflower albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris. We show that both proteins were secreted at high levels and that the purified proteins were properly folded. We also showed that Ber e 1 is glycosylated during secretion and that the glycan does not interfere with the folding or immunoreactivity. The disulphide map of the Ber e 1 protein was experimentally established and is in agreement with the conserved disulphide structure of other members of the 2 S albumin family. A model three-dimensional structure of the allergen was generated. During the expression studies and through mutation we have also shown that alteration of the sequences around the Kex2 endoproteolytic processing site in the expressed fusion protein can compromise the secretion by targeting part of the protein for possible degradation. The secreted production of these properly folded sulphurrich plant albumins presents an opportunity to delineate the attributes that make an allergen and to facilitate the diagnosis and therapy of type I allergy. [less ▲]

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See detailDesign of structural joints in building frames
Jaspart, Jean-Pierre ULg

in Progress in Structural Engineering and Materials (2002), 4(1), 18-34

A single and unified concept for the design of structural joints in building frames made of any material has been developed in recent years, and is now widely implemented in European design codes. In the ... [more ▼]

A single and unified concept for the design of structural joints in building frames made of any material has been developed in recent years, and is now widely implemented in European design codes. In the present paper, this concept is presented, its merits as far as the economy of a project are demonstrated, and practical design tools for its application in daily practice are briefly described. The paper also contains references to user's manuals and design handbooks. [less ▲]

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See detailPierre Bourdieu: une sociologie de la désespérance/ paradigme économique, lutte de pouvoir et "violence inerte"
Robert, Jocelyne ULg

in Revue de l'Institut de Sociologie (2002), 1-4

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See detailCyclin-dependent kinase-2 controls oligodendrocyte progenitor cell cycle progression and is downregulated in adult oligodendrocyte progenitors.
Belachew, Shibeshih ULg; Aguirre, Adan A.; Wang, Hang et al

in Journal of Neuroscience (2002), 22(19), 8553-62

Proliferation of oligodendrocyte progenitor (OP) cells is a crucial process controlling myelination in the CNS. Previous studies demonstrated a correlation between OP proliferation rate and cyclin E ... [more ▼]

Proliferation of oligodendrocyte progenitor (OP) cells is a crucial process controlling myelination in the CNS. Previous studies demonstrated a correlation between OP proliferation rate and cyclin E/cyclin-dependent kinase-2 (cdk2) activity. To establish a causal link between cyclin E/cdk2 activity and OP proliferation, we selectively modulated cdk2 activity in vitro by transfection of cultured OP cells. Dominant-negative (Dn)-cdk2 overexpression inhibited mitogen-induced OP cell proliferation, whereas wild-type (wt)-cdk2 prevented cell cycle arrest caused by anti-mitotic signals. Dn-cdk2- or wt-cdk2-mediated regulation of G(1)/S transition, per se, did not influence initiation of OP differentiation. To study the function of cyclin E/cdk2 in OP cells during development in vivo, we analyzed cdk2 and cyclin E expression in cells acutely isolated from transgenic mice expressing the green fluorescent protein (GFP) under the control of the 2'-3'-cyclic nucleotide 3'-phosphodiesterase gene promoter. Both cyclin E/cdk2 protein levels and activity were decreased in GFP(+) oligodendrocyte lineage cells between postnatal days 4 and 30. Immunostaining of NG2(+)/GFP(+) OP cells in brain tissue sections showed a 90% decrease in overall cell proliferation and cdk2 expression between perinatal and adult cells. However, cdk2 expression within the proliferating (i.e., expressing the proliferating cell nuclear antigen) OP cell population was maintained throughout development. Our data indicate that: (1) cyclin E/cdk2 activity plays a pivotal function in OP cell cycle decisions occurring at G(1)/S checkpoint; (2) initiation of OP differentiation is independent of cyclinE/cdk2 checkpoint, and (3) intrinsic differences in cyclin E/cdk2 expression and activity may underlie the slowly proliferative state that characterizes so-called "quiescent" adult OP cells in vivo. [less ▲]

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See detailThe antitumoral effect of endostatin and angiostatin is associated with a down-regulation of vascular endothelial growth factor expression in tumor cells
Hajitou, Amin; Grignet, Christine ULg; Devy, L. et al

in FASEB Journal (2002), 16

Endostatin and angiostatin are known as tumor-derived angiogenesis inhibitors, but their mechanisms of action are not yet completely defined. We report here that endostatin and angiostatin, delivered by ... [more ▼]

Endostatin and angiostatin are known as tumor-derived angiogenesis inhibitors, but their mechanisms of action are not yet completely defined. We report here that endostatin and angiostatin, delivered by adenoviral vectors, reduced in vitro the neovessel formation in the mouse aortic ring assay by 85 and 40%, respectively. We also demonstrated in vivo that both endostatin and angiostatin inhibited local invasion and tumor vascularization of transplanted murine malignant keratinocytes, and reduced by 50 and 90% the development of highly vascularized murine mammary tumors. This inhibition of tumor growth was associated with a reduction of tumor vascularization. Expression analysis of vascular endothelial growth factor (VEGF) carried out in the mouse aortic ring model revealed a 3- to 10-fold down-regulation of VEGF mRNA expression in endostatin-treated rings. A similar down-regulation of VEGF expression at both mRNA and protein levels was also observed in the two in vivo cancer models after treatment with each angiogenesis inhibitor. This suggests that endostatin and angiostatin effects may be mediated, at least in part, by their ability to down-regulate VEGF expression within the tumor. This work provides evidence that endostatin and angiostatin act on tumor cells themselves. [less ▲]

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See detailLa lecture littéraire avec les faibles lecteurs
De Croix, Séverine ULg; Ledur, Dominique

in Enjeux : Revue de Formation Continuée et de Didactique du Français (2002), 55

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See detailInfluence of a water purification unit on the contamination level of salmonella in outcoming water and sludge
Jacob, Benoit; Korsak Koulagenko, Nicolas ULg; Grooven, Bénédicte et al

in Annales de Médecine Vétérinaire (2002), 146(5), 303-310

Foodborne pathogens occasionally harboured in the gastro-intestinal tract of some domestic animals may be retrieved in slaughterhouses waste water and in sludge of water purification units. Salmonella ... [more ▼]

Foodborne pathogens occasionally harboured in the gastro-intestinal tract of some domestic animals may be retrieved in slaughterhouses waste water and in sludge of water purification units. Salmonella, athogen common to man and Animals, is often used as a biological risk indicator. The aim of the present study was to assess effectiveness of a recent water purification unit by rapid and semi-quantitative detection of this micro-organism. The water purification unit collects waste water of seven food-processing industries, including a pig slaughterhouse. This latest was the main source of Salmonella contamination with a level of more 103 colony forming unit (CFU) per ml, that was very close to the average level of contamination of total incoming waste water. The unit, whose principle of action is based on biological purification, has permitted a 4 log10 reduction of Salmonella contamination, with a final contamination level of less than 1 CFU/ml. It was observed only a little decrease of contamination levels during the biological treatment steps, in contrast with the one observed during the clarification step. This was due to adsorption of bacteria by material in suspension. Fresh sludge were harboured an average of 102 CFU of Salmonella per gram. In the beginning of December 1999, two sludge piles were let on the ground along a field and sampled microbiologically every month. Seven month later, no Salmonella were recovered from the piles. 309 [less ▲]

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See detailHarmonic-Balance Finite-Element Modelling of Electromagnetic Devices: A Novel Approach
Gyselinck, J.; Dular, Patrick ULg; Geuzaine, Christophe ULg et al

in IEEE Transactions on Magnetics (2002), 38(2), 521--524

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See detailPresence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains
Szalo, Ioan Mihai ULg; Goffaux, Frédéric; Pirson, Vinciane et al

in Research in Microbiology (2002), 153(10), 653-658

Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) infections are characterised by the formation of attaching and effacing lesions on intestinal epithelial cells. The first step of ... [more ▼]

Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) infections are characterised by the formation of attaching and effacing lesions on intestinal epithelial cells. The first step of EPEC and EHEC pathogenesis involves the initial adherence of the bacterium to the intestinal epithelium. A collection of bovine EPEC and EHEC strains belonging to different serogroups was tested by colony blot hybridization with gene probes for putative adhesins (BFPA, LPFA, IHA, LIFA) of human EPEC and EHEC, and also for fimbrial and afimbrial adhesins (AFA8, F17, Cs31A) of bovine necrotoxigenic E. coli (NTEC). In the bovine EPEC and EHEC strains tested, sequences homologous to lifA, ihA, and lpfA genes were detected, sometimes in association with particular serogroups. Bovine O26 EPEC also possessed a sequence homologous to a gene of the clp operon, coding for the CS31A adhesin, associated with bovine NTEC. Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains. [less ▲]

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See detailPhylogéographie mitochondriale du mulot sylvestre (Apodemus sylvaticus) dans la région paléarctique occidentale
Michaux, Johan ULg; Magnanou, Elodie; Nieberding, Caroline et al

in Biosystema, Systématique et biogéographie (2002), 20

We have sequenced 965 base pairs of the mitochondrial DNA cytochrome b from 102 woodmice (Apodemus sylvaticus) samples collected from 40 European localities. The aims of the study were to answer the ... [more ▼]

We have sequenced 965 base pairs of the mitochondrial DNA cytochrome b from 102 woodmice (Apodemus sylvaticus) samples collected from 40 European localities. The aims of the study were to answer the following questions: i) Did the Mediterranean peninsulas play a role as a refuge for small mammals? ii) Is the genetic variability of a small mammal like A. sylvaticus higher in the Mediterranean regions as compared with northern Europe? iii)Is it possible to find patterns of postglacial colonisation of Europe other than those presently recognised ? Sequence data were analysed using Distance and Maximum Parsimony phylogenetic reconstruction methods. A minimum spanning network was also calculated. Population genetic structure was determined by analysis of molecular variance (AMOVA). A "mismatch distribution" analysis was also performed to estimate the patterns of expansion. The results provide a clear picture of the impact of Quaternary glaciations on the genetic and geographic structure of the woodmouse. Analyses indicate a higher genetic variability for the woodmouse in the Mediterranean peninsulas as compared to northern Europe and the role of these peninsulas as refuge regions for small mammals. A new pattern of postglacial colonisation is also proposed where the Iberian and southern France refuge populations colonised almost all the European regions. The Sicilian population appears to be highly differentiated and highly variable. This result emphasises the importance of this island as a "hot spot" for the intraspecific genetic diversity of the woodmouse. Finally, populations of this species in North Africa originated from south-western Europe and are probably the result of a recent anthropogenic introduction. [less ▲]

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See detailSelf-adapting time integration management in crash-worthiness and sheet metal forming computations
Noels, Ludovic ULg; Stainier, Laurent ULg; Ponthot, Jean-Philippe ULg

in International Journal of Vehicle Design (2002), 30(1-2), 67-114

Variable step strategies are especially well suited to deal with problems characterized by high non-linearity and contact/impact, and resolved with an implicit scheme. Both phenomena are typical of ... [more ▼]

Variable step strategies are especially well suited to deal with problems characterized by high non-linearity and contact/impact, and resolved with an implicit scheme. Both phenomena are typical of dynamic simulations of contact-impact problems, as well as sheet metal forming. Constant step size strategies do not give a satisfactory answer for this kind of problem, since it is very difficult, if not impossible, for the user to find an appropriate time step that does not lead to divergence nor generate extremely costly computations. An automatic time stepping algorithm is proposed, which takes into account the recent history of accelerations in the deformable bodies under consideration. More precisely, the adaptation algorithm is based on estimators of the integration error of the differential dynamic balance equations. This allows for adaptation of the step size to capture correctly the transient phenomena, with characteristic times which can range from relatively long (after contact, or during sheet metal forming) to very short (during contact-impact). thus ensuring precision while keeping the computation cost to a minimum. Furthermore, we will see that this strategy can be used in explicit schemes. Additionally, the proposed algorithm automatically takes decisions regarding the necessity of updating the tangent matrix or stopping the iterations, further reducing the computational costs especially, when the Augmented Lagrangian method is used. As an illustration of the capabilities of this algorithm, several numerical simulations (shock absorber devices for vehicle crash-worthiness or sheet metal forming) problems will be presented. Other simulations pertaining to the sheet metal forming for vehicle structures will also be investigated, thus demonstrating the versatility, the capabilities and the efficiency of the proposed strategy. [less ▲]

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See detailThe effect of an acute energy deficit on the hormone profile of dominant follicles in dairy cows
Comin, Antonella; Gerin, Daniele; Cappa, Alberto et al

in Theriogenology (2002), 58

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See detailComment je traite.... Un pityriasis rubra pilaire.
Fraiture, A. L.; Braham, C.; Pierard, Claudine ULg et al

in Revue Médicale de Liège (2002), 57(6), 363-5

Pityriasis rubra pilaris is a syndrome of unknown etiology. Six distinct clinical types have been described in infancy and adulthood. Oral retinoids have proven their efficacy. Emollients and topical ... [more ▼]

Pityriasis rubra pilaris is a syndrome of unknown etiology. Six distinct clinical types have been described in infancy and adulthood. Oral retinoids have proven their efficacy. Emollients and topical calcipotriol may improve the clinical presentation. [less ▲]

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See detailLes acteurs et les enjeux au coeur de l'évaluation
Vandoorne, Chantal ULg

in Education santé (2002), 173(173), 9-11

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See detailLe langage des personnes âgées porteuses d'une trisomie 21
Rondal, Jean-Adolphe ULg; Comblain, Annick ULg

in Orthophonie et Logopédie (2002), 1

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See detailFcpac: Fast Calculation Of Partial Atomic Charges Using Strictly Localized Molecular Orbitals
Swinnen, M.; Thomas, Annick ULg; Brasseur, Robert ULg

in Computational Materials Science (2002), 25(4), 590-595

Most force fields of molecular mechanics use constant partial atomic charges whereas it is now admitted that those charges strongly vary with the environment. Charge variations are particularly important ... [more ▼]

Most force fields of molecular mechanics use constant partial atomic charges whereas it is now admitted that those charges strongly vary with the environment. Charge variations are particularly important in hydrogen bonds where polar entities come close together. Moreover, organizing H-bonds in networks, like in secondary structures of proteins, go with cooperative effects which lead to an overall electrostatic stabilization of the system. This is why variable atomic charges are required to correctly mimic these effects. Different methods of charge calculation were developed, most use polarizable dipole or electronegativity equalization. In this work, we propose a semi-empirical method (fast calculation of partial atomic charges (FCPAC)) which derives partial atomic charges from strictly localized molecular orbitals. The approximations enable to treat molecular systems with hundreds of atoms within reasonable delays. Results reported here show that charges calculated with FCPAC are similar to charges derived from Mulliken’s population analysis of a restricted Hartree–Fock wave function using the split valence basis set 6-31G and including polarization orbitals p and d respectively for hydrogen and heavy atoms, RHF/6-31G(d,p). Comparison with ab initio calculation was also performed on a system composed of five formamide molecules disposed in the same geometry as peptide bonds in an a helix. Charges variations are similar and suggest that FCPAC is suitable for quantitative estimation of cooperative effects. [less ▲]

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See detailMagnetic properties of Ga-doped lanthanum manganite with controlled oxygen stoichometry
Vertruyen, Bénédicte ULg; Hebert, Sylvie; Maignan, Antoine et al

in Crystal Engineering (2002), 5

Stoichiometric LaMnO3 is an insulator whose A-type antiferromagnetic structure at low temperature is mainly connected to the Jahn-Teller distortion of the MnO6 octahedra. The substitution of non magnetic ... [more ▼]

Stoichiometric LaMnO3 is an insulator whose A-type antiferromagnetic structure at low temperature is mainly connected to the Jahn-Teller distortion of the MnO6 octahedra. The substitution of non magnetic d(10) Ga3+ ions on the manganese site disrupts the orbital and spin orderings of the manganese ions and has a strong influence on the physical properties. The antiferromagnetic order is progressively destroyed while the ferromagnetic component increases. At the same time, the distortion of the orthorhombic cell decreases and the high temperature structural Jahn-Teller transition disappears. Such studies on manganese site substitutions give insight into the physics of the Mn-O three-dimensional network, which is supposed to be the key feature in the CMR manganites. (C) 2003 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailRespiratory feedback in the generation of emotion
Philippot, P; Chapelle, G; Blairy, Sylvie ULg

in Cognition & Emotion (2002), 15(5), 605-627

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See detailComparison of Ricotta cheese made by high pressure treatment with that produced by heat treatment of sweet whey
Besbes, S.; Blecker, Christophe ULg; Attia, H. et al

in Sciences des Aliments (2002), 22(5), 601-615

In this study, reconstituted sweet whey was acidified and then treated by high hydrostatic pressure in order to obtain Ricotta-like product. At pH 5.2, whey protein aggregation was effective beyond 200 ... [more ▼]

In this study, reconstituted sweet whey was acidified and then treated by high hydrostatic pressure in order to obtain Ricotta-like product. At pH 5.2, whey protein aggregation was effective beyond 200 MPa for an application time of 30 min. Moist yield was appreciably improved when pressurisation time increased at 400 MPa. It seemed that granulometric properties of Ricotta-like cheese were not affected by increasing pressurisation time. Ricotta-like products obtained after pressurisation at 400 MPa for 30, 45 and 60 min, have shown a lower yield and hardness but a higher cohesiveness than traditional Ricotta cheese. They also had more homogenous granulometric profiles. [less ▲]

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See detailUse of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals
Willemsen, Philippe; Scippo, Marie-Louise ULg; Maghuin-Rogister, Guy ULg et al

in Analytica Chimica Acta (2002), 473(1-2), 119-126

We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of ... [more ▼]

We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of steroid agonists and antagonists down to 0.3 ng g(-1) is described and its application to the analysis of bovine samples is discussed. In particular, the use of the assays to detect illegal progesterone or androgen treatment has to take into account the presence of natural endogenous hormones. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailKinetic study of the acid hydrolysis of various oligofructose samples
Blecker, Christophe ULg; Fougnies, C.; Van Herck, J. C. et al

in Journal of Agricultural and Food Chemistry (2002), 50(6), 1602-1607

The kinetic of acid hydrolysis of five commercially available oligofructose samples used as food ingredients has been investigated as a function of the dry matter concentration, reaction pH, and ... [more ▼]

The kinetic of acid hydrolysis of five commercially available oligofructose samples used as food ingredients has been investigated as a function of the dry matter concentration, reaction pH, and temperature. The initial fructose release rate is found to be roughly proportional to the inverse of the average polymerization degree in number. A pseudo first order kinetic is found with respect to the fructosyl chain end concentration and to the proton concentration. Arrhenius plot is found to reasonably fit the data in a relatively wide temperature range (7-130 degreesC). The results allow the estimation of the fructose release rate in many foodstuff processing conditions. [less ▲]

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See detailGeochemistry of bitter brines in the Salar de Coipasa - Bolivia
Lebrun, Vincent; Pacosillo, Pedro; Gutierrez, Jorge et al

in Aardkundige Mededelingen (2002), 12

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See detailLes figures féminines dans le théâtre de Jean-Marie Piemme
Delhalle, Nancy ULg

in Alternatives Théâtrales (2002), (75),

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See detailClonal diversity and metallo-beta-lactamase production in clinical isolates of Stenotrophomonas maltophilia
Mercuri, Paola ULg; Ishii, Y.; Ma, L. et al

in Microbial Drug Resistance : Mechanism, Epidemiology, & Disease (2002), 8(3), 193-200

Stenotrophomonas maltophilia is a nosocomial pathogen with an intrinsic broad-spectrum resistance to beta-lactam compounds and other antibacterial agents. It produces two chromosomal beta-lactamases: a ... [more ▼]

Stenotrophomonas maltophilia is a nosocomial pathogen with an intrinsic broad-spectrum resistance to beta-lactam compounds and other antibacterial agents. It produces two chromosomal beta-lactamases: a clavulanic acid-sensitive class A (L2) and a tetrameric carbapenemase (L1 or BlaS). We screened 40 S. maltophilia multidrug-resistant clinical isolates recovered between 1995 and 1998 in the Varese Hospital (Italy) for the presence of the metallo-beta-lactamase. The isolates were investigated by phenotypic profiling (enzymatic activity and antibiotic resistance pattern) and molecular methods such as PCR and pulsed-field gel electrophoresis (PFGE) to reveal intraspecies diversity. For the tested S. maltophilia strains, we showed that the beta-lactamase production could be induced by the presence of imipenem (50 mug/ml) in the culture media. Addition of 1 mM dipicolinic acid completely inhibited the hydrolysis of imipenem but decreased that nitrocefin in a strain-dependent manner. Full activity of crude extract towards imipenem could be restored by addition of 1 mM ZnCl2. Finally, the gene encoding the carbapenem-hydrolyzing beta-lactamase from S. maltophilia ULA-511 and 39/95, a clinical strain, were isolated and sequenced. These two strains have a different profile of multidrug resistance. The two metallo-beta-lactamases were found to be isologous. The difference of sensitivity of these two strains was associated to the level of production of the metallo-beta-lactamase. [less ▲]

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See detailSyndrome cérébelleux paradoxal provoqué par un méningiome chez une vache
Danlois, F.; Poncelet, Luc; Rollin, Frédéric ULg

in Annales de Médecine Vétérinaire (2002), 146

A paradoxical vestibular syndrome caused by a fibroblastic meningioma is described in a 20 month-old Holstein heifer. Clinical signs included head tilt, jerking movements of the head and neck, circling to ... [more ▼]

A paradoxical vestibular syndrome caused by a fibroblastic meningioma is described in a 20 month-old Holstein heifer. Clinical signs included head tilt, jerking movements of the head and neck, circling to the left, and also abducted and hypermetric right limbs. The animal was culled and the forebrain therefore was unavailable for histopathological study. The tumour was found on the right side, adherent to the dura mater at the level of the pontomedullary junction. The paradoxical vestibular signs were explained by the presence of the mass causing a loss of cerebellar inhibition over homolateral vestibular nuclei resulting in hyperactivity on the same side as the tumour and simulating a contralateral functional loss. The jerking head and neck movements might be caused by the same lesion although a concurrent forebrain lesion could not be excluded. [less ▲]

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See detailEuro- en zijn concurrenten
Meesters, Gert ULg

in Leuvense Bijdragen (2002), 91(1-2), 79-96

This article deals with the Dutch bound morpheme euro-, meaning ‘European’ or ‘Europe’ in relatively young word formations. The morpheme in question is not to be confounded with the word euro for the ... [more ▼]

This article deals with the Dutch bound morpheme euro-, meaning ‘European’ or ‘Europe’ in relatively young word formations. The morpheme in question is not to be confounded with the word euro for the European currency. The article especially deals with the relationship between word formation with euro- and three other means of expressing more or less the same meaning: word formation with EG- (abbreviation of Europese Gemeenschap, i.e. European Union) as a first component, word formation with Europa- (Europe) as a first component and word groups with the adjective Europees (European). This relationship is explored in two comparative studies. First the token frequency in a corpus is investigated. It turns out that euro- is more frequent than Europa-, but less frequent than EG- and Europees. In a second comparison the semantic-functional domains of the four alternatives are considered in detail. Euro- apparently can be used as an alternative for Europees and EG- when the referents are related to the European Union, and as an alternative for Europees in ‘bilateral’ adjectives such als Euro-Russisch. It also has a few domains of its own: commercial proper names and words expressing an attitude towards the European Union. An additional analysis of individual words and word groups with different alternatives from this paradigm as a first component but the same second component reveals that even in individual cases of competition with other alternatives, the variant with euro- is sometimes the most frequent or the institutionalized form. It is concluded that within the linguistic potential of naming referents that relate to Europe, euro- has its own specific value as a productive means of word formation. [less ▲]

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See detailCommunication et langage chez les animaux.
Poncin, Pascal ULg; Huynen, Marie-Claude ULg; Ruwet, J.Cl.

in Bulletin de la Société Royale des Sciences de Liège (2002), 4

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See detailBiocontrol Of Listeria Monocytogenes In A Model Cultured Milk (Lben) By In Situ Bacteriocin Production From Lactococcus Lactis Ssp Lactis
Benkerroum, N.; Ghouati, Y.; Ghalfi, H. et al

in International Journal of Dairy Technology (2002), 55(3),

Bacteriocin-producing (Bac+) Lactococcus lactis ssp. lactis CCMM/IAV/BK1 isolated from traditional lben was used in the preparation of lben from pasteurized milk to assess its potential inhibitory ... [more ▼]

Bacteriocin-producing (Bac+) Lactococcus lactis ssp. lactis CCMM/IAV/BK1 isolated from traditional lben was used in the preparation of lben from pasteurized milk to assess its potential inhibitory activity against Listeria monocytogenes ATCC 7644. Production of bacteriocin (arbitrary units, AU) in MRS broth fortified with yeast extract (MRSY) in a fermentor under controlled and uncontrolled pH conditions was also investigated. This Bac+ strain yielded about 35 times more bacteriocin when the pH was maintained constant at 6.5 than under varying pH conditions. To test the effect of in situ bacteriocin production against L. monocytogenes, lben was made from cow's milk artificially contaminated with approximately 107 cfu/mL and fermented with a mixed mesophilic starter culture consisting of the lactococcal Bac+ organism and Lc. lactis ssp. lactis biovar diacetylactis 66, a diacetyl-producing strain, in a ratio of 1 : 1. Numbers of L. monocytogenes were monitored during fermentation and storage of lben at refrigeration temperature (c. 7°C) for up to 6 days. Performances of the Bac+ starter were compared to those of an isogenic Bac- derivative strain obtained from the Bac+ starter by curing with ethidium bromide. The results showed that the amount of L. monocytogenes decreased to below the detectable level in a 1-mL sample within 24 h of storage at 7°C in lben fermented with the Bac+ starter culture. On the contrary, L. monocytogenes survived for 6 days of storage at 7°C in lben made with the Bac- starter. The Bac+ wild strain of the starter studied could be adequately used to produce lben or similar indigenous fermented milks of improved hygienic quality on an industrial scale. Alternatively, it could be used as an adjunct in minimally processed products or in products obtained from raw milk to add a safety factor. [less ▲]

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See detailL’arrêt n°41/2002 de la Cour d’arbitrage : de la notion constitutionnelle de droit politique à la notion de "droit civil" au sens de la Convention européenne des droits de l’homme
Rosoux, Géraldine ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2002)

The article analyzes the impact of the conventional concept of "civil right" (art. 6 E.C.H.R.) on the constitutional distinction between civil and political rights (art. 144-145 Const.).

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See detailBelgian-style Human Resource Management: A Case of Mistaken Identity
Claus, Lisbeth; Vloeberghs, Daniel; Pichault, François ULg

in European Management Journal (2002), 20(4), 438-446

Discusses various forces that affect the future of human resource management (HRM) in Belgium. Development of HRM in the country; Specific forces that have shaped the development and character of HRM ... [more ▼]

Discusses various forces that affect the future of human resource management (HRM) in Belgium. Development of HRM in the country; Specific forces that have shaped the development and character of HRM; Details of the condition of HRM in the country. [less ▲]

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See detailLa régulation des services publics: les enseignements du droit comparé
Thirion, Nicolas ULg

in Chroniques de Droit Public = Publiekrechtelijke Kronieken (2002), 4

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See detailLittérature dialectale à Farciennes et Atlas linguistique de la Wallonie. De la géographie à la généricité
Droixhe, Daniel ULg; Vanwelkenhuyzen, Nadine ULg

in Revue Belge de Philologie et d'Histoire (2002), 80(3), 875-904

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See detailChoisir un scénario sylvicole pour les mélèzes
Pauwels, D.; Lejeune, Philippe ULg; Rondeux, Jacques ULg

in Forêt Wallonne (2002), (61), 17-24

Le choix du traitement sylvicole à appliquer à un peuplement forestier est une démarche qui peut s’avérer très complexe si l’on tient compte des délais entre la plantation et la récolte du peuplement ... [more ▼]

Le choix du traitement sylvicole à appliquer à un peuplement forestier est une démarche qui peut s’avérer très complexe si l’on tient compte des délais entre la plantation et la récolte du peuplement final, de l’évolution des filières de transformation, ou encore de la multiplicité des objectifs que peut poursuivre le gestionnaire forestier. [less ▲]

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See detailLa mesure de la pression artérielle : écueils et chausse-trapes
Rorive, Georges ULg; Dubois, Bernard ULg; Saint-Remy, Annie ULg

in Revue Médicale de Liège (2002), 57(5), 250-252

La mesure de la pression artérielle est probablement l'acte médical le plus souvent réalisé et certainement un des moins fiables. L'introduction de la mesure ambulatoire de la pression artérielle a permis ... [more ▼]

La mesure de la pression artérielle est probablement l'acte médical le plus souvent réalisé et certainement un des moins fiables. L'introduction de la mesure ambulatoire de la pression artérielle a permis d'identifier l'importance de l'effet consultatio et, par là, de définir l'hypertension de "la blouse blanche". Celle-ci se définit comme une pression artérielle pathologique à la consultation et strictement normale en dehors. Si on la définit de cette manière, cette pathologie ne s'accompagne pas d'une augmentation de la fréqunce des affections cardiovasculaires. Chez un tiers des patients, cependant, elle évolue vers des formes plus sévères de l'hypertension. [less ▲]

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See detailEtude de la perturbation de l'image du corps dans un échantillon de patientes boulimiques
Bragard, Isabelle ULg; Etienne, Anne-Marie ULg; Mayers, I. et al

in Revue Francophone de Clinique Comportementale et Cognitive (2002), 7(2), 5-18

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See detailEffect of some insecticides on acetylcholinesterase from beneficial insects: Coccinella septempunctata, Chrysoperla carnea and Forficula auricularia
Boszic, Andras; Francis, Frédéric ULg; Gaspar, Charles et al

in Med. Fac. Landbouw. Univ. Gent (2002), 67(3), 671-677

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See detailAutorité parentale et actes médicaux
Leleu, Yves-Henri ULg; Delval, S.

in Journal du Droit des Jeunes (2002)

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See detailEcological network and local authorities - sociological instrument
Mougenot, Catherine ULg; Roussel, Laurence

in Nature and environment (2002), (126), 37

Ever closer links have been forged between caring for nature on the one hand and human activities on the other. In recent years there has been a shift from an interest in reserve-based nature, which ... [more ▼]

Ever closer links have been forged between caring for nature on the one hand and human activities on the other. In recent years there has been a shift from an interest in reserve-based nature, which excluded man and human activities, to an increasingly powerful nature-culture relationship. Nature protection became nature conservation and then nature development with the integration of socio-economic and cultural aspects. Within the framework of the setting-up of the pan-european ecological network, these sociological aspects have a great importance for establishing an ecological network where the scientific aspects of biodiversity protection must co-exist with nature management and development. [less ▲]

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See detailImpaired emotional facial expression recognition is associated with interpersonal problems in alcoholism
Kornreich, C; Philippot, P; Foisy, ML et al

in Alcohol & Alcoholism (2002), 37(4), 394-400

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See detailAdvantages and drawbacks of nanospray for studying noncovalent protein-DNA complexes by mass spectrometry
Gabelica, Valérie ULg; Vreuls, Christelle ULg; Filée, Patrice ULg et al

in Rapid Communications in Mass Spectrometry : RCM (2002), 16(18), 1723-1728

The noncovalent complexes between the BlaI protein dimer (wild-type and GM2 mutant) and its double-stranded DNA operator were studied by nanospray mass spectrometry and tandem mass spectrometry (MS/MS ... [more ▼]

The noncovalent complexes between the BlaI protein dimer (wild-type and GM2 mutant) and its double-stranded DNA operator were studied by nanospray mass spectrometry and tandem mass spectrometry (MS/MS). Reproducibility problems in the nanospray single-stage mass spectra are emphasized. The relative intensities depend greatly on the shape of the capillary tip and on the capillary-cone distance. This results in difficulties in assessing the relative stabilities of the complexes simply from MS' spectra of protein-DNA mixtures. Competition experiments using MS/MS are a better approach to determine relative binding affinities. A competition between histidine-tagged BlaIWT (BlaIWTHis) and the GM2 mutant revealed that the two proteins have similar affinities for the DNA operator, and that they co-dimerize to form heterocomplexes. The low sample consumption of nanospray allows MS/MS spectra to be recorded at different collision energies for different charge states with 1 muL of sample. The MS/MS experiments on the dimers reveal that the GM2 dimer is more kinetically stable in the gas phase than the wild-type dimer. The MS/MS experiments on the complexes shows that the two proteins require the same collision energy to dissociate from the complex. This indicates that the rate-limiting step in the monomer loss from the protein-DNA complex arises from the breaking of the protein-DNA interface rather than the protein-protein interface. The dissociation of the protein-DNA complex proceeds by the loss of a highly charged monomer (carrying about two-thirds of the total charge and one-third of the total mass). MS/MS experiments on a heterocomplex also show that the two proteins BlaIWTHis and BlaIGM2 have slightly different charge distributions in the fragments. This emphasizes the need for better understanding the dissociation mechanisms of biomolecular complexes. [less ▲]

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See detailMolecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues
Lakaye, Bernard ULg; Makarchikov, Alexander F; Antunes, Adelio F et al

in Journal of Biological Chemistry (2002), 277(16), 13771-13777

Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues, and recent data suggest that it may act as a phosphate donor for the phosphorylation of some proteins. In the mammalian ... [more ▼]

Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues, and recent data suggest that it may act as a phosphate donor for the phosphorylation of some proteins. In the mammalian brain, ThTP synthesis is rapid, but its steady-state concentration remains low, presumably because of rapid hydrolysis. In this report we purified a soluble thiamine triphosphatase (ThTPase; EC 3.6.1.28) from calf brain. The bovine ThTPase is a 24-kDa monomer, hydrolyzing ThTP with virtually absolute specificity. Partial sequence data obtained from the purified bovine enzyme by tandem mass spectrometry were used to search the GenBank(TM) data base. A significant identity was found with only one human sequence, the hypothetical 230-amino acid protein MGC2652. The coding regions from human and bovine brain mRNA were amplified by reverse transcription-PCR, cloned in Escherichia coli, and sequenced. The human open reading frame was expressed in E. coli as a GST fusion protein. Transformed bacteria had a high isopropyl-beta-D-thiogalactopyranoside-inducible ThTPase activity. The recombinant ThTPase had properties similar to those of human brain ThTPase, and it was specific for ThTP. The mRNA was expressed in most human tissues but at relatively low levels. This is the first report of a molecular characterization of a specific ThTPase. [less ▲]

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See detailDevelopment of GEQUAC as a new group contribution method for strongly non-ideal mixtures
Ehlker, Gerhard H; Pfennig, Andreas ULg

in Fluid Phase Equilibria (2002), 203((1-2)), 53-69

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See detailLes antagonistes calciques ont-ils une place de première ligne dans le traitement de l'hypertension artérielle?
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (2002), 57(2), 79-84

During the nineties, a controversy was noted in the literature about the potential risk of using calcium antagonists in the treatment of hypertension. Several recent studies, such as the INSIGHT’s one ... [more ▼]

During the nineties, a controversy was noted in the literature about the potential risk of using calcium antagonists in the treatment of hypertension. Several recent studies, such as the INSIGHT’s one have not confirmed this provided that one uses long-acting molecule. But the real beneficial effect due to the different antihypertensive classes is mainly linked to the magnitude of the blood pressure lowering effect. [less ▲]

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See detailRadiologic features poorly predict clinical outcomes in knee osteoarthritis.
Bruyère, Olivier ULg; Honore, Aline; Rovati, Lucio C et al

in Scandinavian Journal of Rheumatology (2002), 31(1), 13-6

OBJECTIVES: To assess the impact of radiographic severity and progression on pain and disability. METHODS: Measurements of mean joint space width (JSW), narrowest join space (NJS) point and assessment of ... [more ▼]

OBJECTIVES: To assess the impact of radiographic severity and progression on pain and disability. METHODS: Measurements of mean joint space width (JSW), narrowest join space (NJS) point and assessment of symptoms by the WOMAC questionnaire were performed at baseline and after three years in 212 subjects over 50 years with primary knee OA. RESULTS: At baseline, JSW and NJS were not significantly correlated with the scores recorded for the WOMAC global index or its pain, stiffness or function subscales. A statistically significant correlation was observed between the joint space narrowing over three years and the changes observed in the pain subscale of the WOMAC during the same period. The three-year changes in the global WOMAC index in patients within the lowest and the highest quartiles of mean joint space width at baseline showed, in both cases, a statistically (p<0.05) significant favorable difference between patients treated with glucosamine sulphate and those having received placebo. CONCLUSION: Radiographic and clinical progressions of the disease are significantly associated but the clinical relevance of the association is questionable. [less ▲]

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See detailOncoviral bovine leukemia virus G4 and human T-cell leukemia virus type 1 p13(II) accessory proteins interact with farnesyl pyrophosphate synthetase
Lefebvre, Laurent; Vanderplasschen, Alain ULg; Ciminale, Vincenzo et al

in Journal of Virology (2002), 76(3), 1400-1414

G4 and p13(II) are accessory proteins encoded by the X region of bovine leukemia virus and human T-cell leukemia virus type 1 (HTLV-1), respectively. Disruption of the G4 and p13(II) open reading frames ... [more ▼]

G4 and p13(II) are accessory proteins encoded by the X region of bovine leukemia virus and human T-cell leukemia virus type 1 (HTLV-1), respectively. Disruption of the G4 and p13(II) open reading frames interferes with viral spread in animal model systems, indicating that the corresponding proteins play a key role in viral replication. In addition, G4 is oncogenic in primary cell cultures and is absolutely required for efficient onset of leukemogenesis in sheep. To gain insight into the function of these proteins, we utilized the yeast two-hybrid system to identify protein partners of G4. Results revealed that G4 interacts with farnesyl pyrophosphate synthetase (FPPS), a protein involved in the mevalonate/squalene pathway and in synthesis of FPP, a substrate required for prenylation of Ras. The specificity of the interaction was verified by glutathione S-transferase (GST) pull-down assays and by coimmunoprecipitation experiments. Furthermore, confocal microscopy showed that the subcellular localization of G4 was profoundly affected by FPPS. The G4 protein itself was not prenylated, at least in rabbit reticulocyte lysate-based assays. The domain of G4 required for binding to FPPS was restricted to an amphipathic alpha-helix rich in arginine residues. Subtle mutation of this alpha-helix abrogated G4 oncogenic potential in vitro, providing a biological relevance for FPPS-G4 complex formation in cells. Finally, HTLV-1 p13(II) was also found to specifically interact with FPPS (in yeast as well as in GST pull-down assays) and to colocalize with G4 in mitochondria, suggesting a functional analogy between these oncoviral accessory proteins. Identification of FPPS as a molecular partner for p13(II) and G4 accessory proteins opens retrovirus-induced leukemia. [less ▲]

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See detailInfluence of a New Axial Impeller on KLa and Xylanase Production by Penicillium canescens 10-10c
Bakri, Y.; Jacques, Philippe ULg; Shi, L.K. et al

in Applied Biochemistry and Biotechnology (2002), (98-100), 679-686

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See detailLes adhésifs de cinquième génération
Gueders, Audrey ULg

in Actualités Dentaires de l'Université de Liège (2002), 30

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See detailOxygen consumption and electron spin resonance studies of free radical production by alveolar cells exposed to anoxia: inhibiting effects of the antibiotic ceftazidime.
Mouithys-mickalad, A.; Mathy-hartert, M.; Du, G. et al

in Redox Report : Communications in Free Radical Research (2002), 7(2), 85-94

By EPR spectroscopy, we investigated free radical production by cultured human alveolar cells subjected to anoxia/re-oxygenation (A/R), and tested the effects of ceftazidime, an antibiotic previously ... [more ▼]

By EPR spectroscopy, we investigated free radical production by cultured human alveolar cells subjected to anoxia/re-oxygenation (A/R), and tested the effects of ceftazidime, an antibiotic previously demonstrated to possess antioxidant properties. Two A/R models were performed on type II pneumocytes (A549 cell line), either on cells attached to culture dishes (monolayer A/R model; 3.5 h of anoxia, 30 min of re-oxygenation) or after cell detachment (suspension A/R model; 1 h of anoxia, 10 min of re-oxygenation). Ceftazidime and selective inhibitors (SOD, Tiron, L-NMMA) were added before anoxia. Free radical production was assessed by the EPR spin trapping technique. Oxygen consumption was monitored, in parallel with EPR studies, in the suspension A/R model. The production of free radical species was demonstrated by the generation of PBN-radical adducts: (a(N) = 15.2 G) in the monolayer A/R model and a six-line EPR spectrum (a(N) = 15.7 G and a(H) = 2.7 G) in the suspension A/R model. A kinetic study performed by oximetry, in parallel with EPR spectroscopy, demonstrated marked alterations of the cell respiratory function and that the free radical production started during anoxia and increased during re-oxygenation. In the suspension A/R model, the amplitude of EPR spectra were decreased upon the addition of 200 U/ml SOD (37% inhibition), 0.1 mM Tiron (67% inhibition) and 1 mM L-NMMA (43% inhibition). Addition of 1 mM ceftazidime decreased the amplitude of EPR spectra (37% inhibition) in both A/R models. Complementary in vitro EPR studies demonstrated that CAZ scavenged the hydroxyl radical (produced by the Fenton reaction). The protective effect of ceftazidime in the cell model could thus be linked to its ability to scavenge superoxide anions, nitrogen-derived species and hydroxyl radicals. [less ▲]

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See detailLe mélange épicéa-douglas, une association intéressante pour le sylviculteur ? 2e partie
Colson, Vincent ULg; Claessens, Hugues ULg; Pauwels, D. et al

in Silva Belgica (2002), 109(4), 25-31

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See detailQuantitative and qualitative estimation of bacteria in foodstuffs by genetic amplification
China, Bernard; Ghafir, Yasmine; Daube, Georges ULg

in Annales de Médecine Vétérinaire (2002), 146(2, APR-MAY),

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed ... [more ▼]

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed until now require the growth of the microorganism on a selective medium allowing to differentiate it from other bacteria. This procedure could be long and difficult for the analysis. To improve these classical methods, molecular biology techniques based on the specific amplification of a DNA sequence are in development. Moreover, the apparition of real time PCR allows new future prospects since it allows to quantify the number of bacteria present in a sample. This quantitative aspect is very important in food microbiology. In this way, the molecular biology tools should allow to develop fast, sensitive, specific and quantitative diagnosis tests to confirm the results obtained by classical methods but also to allow the diagnosis of fastidious bacteria. [less ▲]

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See detailIn vivo manganese-enhanced magnetic resonance imaging reveals connections and functional properties of the songbird vocal control system
Van der Linden, A.; Verhoye, M.; Van Meir, V. et al

in Neuroscience (2002), 112(2), 467-474

Injection of manganese (Mn2+), a paramagnetic tract tracing agent and calcium analogue, into the high vocal center of starlings labeled within a few hours the nucleus robustus archistriatalis and area X ... [more ▼]

Injection of manganese (Mn2+), a paramagnetic tract tracing agent and calcium analogue, into the high vocal center of starlings labeled within a few hours the nucleus robustus archistriatalis and area X as observed by in vivo magnetic resonance imaging. Structures highlighted by Mn2+ accumulation assumed the expected tri-dimensional shape of the nucleus robustus archistriatalis and area X as identified by classical histological or neurochemical methods. The volume of these nuclei could be accurately calculated by segmentation of the areas highlighted by Mn2+. Besides confirming previously established volumetric sex differences, Mn2+ uptake into these nuclei revealed new functional sex differences affecting Mn2+ transport. A faster transport was observed in males than in females and different relative amounts of Mn2+ were transported to nucleus robustus archistriatalis and area X in males as compared to females. This new in vivo approach, allowing repeated measures, opens new vistas to study the remarkable seasonal plasticity in size and activity of song-control nuclei and correlate neuronal activity with behavior. It also provides new insights on in vivo axonal transport and neuronal activity in song-control nuclei of oscines. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailIntragranular and intergranular behaviour of multifilamentary Bi-2223/Ag tapes
Fagnard, Jean-François ULg; Vanderbemden, Philippe ULg; Crate, David et al

in Physica C: Superconductivity (2002), 372(Part 2), 970-973

This communication aims at reporting the superconducting properties of Bi-2223/Ag tapes determined by using various measuring techniques. First. the original samples have been characterized by electrical ... [more ▼]

This communication aims at reporting the superconducting properties of Bi-2223/Ag tapes determined by using various measuring techniques. First. the original samples have been characterized by electrical resistance, AC susceptibility. and DC magnetization. The transport (intergranular) critical Current vs. magnetic field was also determined at T = 77 K using pulsed currents up to 40 A. Next. the same combination of experiments as performed oil bent tapes in order to bring out relevant information concerning the strength of the intergranular coupling. The results show that intergranular and intragranular currents differ by at least one order of magnitude, Finally, additional magnetic measurements were carried out in order to determine the anisotropy ratio Jc(ab)/Jc(c), which was found to lie around 30. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailReconstruction of healthcare in Afghanistan.
Porignon, Denis ULg; Hennart, Philippe

in Lancet (2002), 359(9311), 1071-2

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See detailPourquoi la Finlande a-t-elle tant de jeunes bons lecteurs ?
Lafontaine, Dominique ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2002), 9-10

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See detailL'hyperglycemie post-prandiale. I. Physiopathologie, consequences cliniques et approaches dietetiques.
Scheen, André ULg; Paquot, Nicolas ULg; Jandrain, Bernard ULg et al

in Revue Médicale de Liège (2002), 57(3), 138-41

Postprandial hyperglycaemia depends on the amount and type of ingested carbohydrates and/or the degree of inhibition of hepatic glucose output following a meal. The kinetics of carbohydrate absorption is ... [more ▼]

Postprandial hyperglycaemia depends on the amount and type of ingested carbohydrates and/or the degree of inhibition of hepatic glucose output following a meal. The kinetics of carbohydrate absorption is directly influenced by the type of food (carbohydrates with variable glycaemic indices, fibre content of the meal) and by the speed of gastric emptying. Hepatic glucose output is remarkably inhibited by insulin and strongly stimulated by glucagon. It remains abnormally high after a meal in diabetic patients because of insufficient portal insulin concentrations, hepatic insulin resistance and/or hyperglucagonaemia. In diabetic patients, postprandial hyperglycaemia contributes to the aggravation of chronic hyperglycaemia, and thus to the increase of glycated haemoglobin levels. Furthermore, it has been recently demonstrated that postprandial hyperglycaemia increases the cardiovascular risk, even in nondiabetic subjects, probably by inducing endothelial dysfunction. Appropriate dietary counselling plays a key-role in the control of postprandial hyperglycaemia. Generally speaking, it includes a selection of carbohydrates with low glycaemic index and a higher fibre intake. Pharmacological interventions may also be considered when necessary. [less ▲]

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See detailOptimal timing of elective caesarean section in Belgian White and Blue breed of cattle: The calf's point of view
Uystepruyst, Christophe; Coghe, Joost; Dorts, Thierry et al

in Veterinary Journal (2002), 163(3), 267-282

The purpose of this study was to evaluate whether the timing of elective caesarean section (ECS) during parturition affects pulmonary and metabolic adaptation to extra-uterine life in healthy Belgian ... [more ▼]

The purpose of this study was to evaluate whether the timing of elective caesarean section (ECS) during parturition affects pulmonary and metabolic adaptation to extra-uterine life in healthy Belgian White and Blue (BWB) calves delivered at term. Vaginal palpation was performed and deliveries divided into six categories of timing for ECS: cervix closed (TECS 1) passive and active cervical dilatation (TECS 2 and TECS 3); full cervical dilatation (TECS 4); spontaneous rupture of allantoic (TECS 5) and amniotic (TECS 6) membranes. One hundred and eighteen BWB calves were examined at birth, 5, 15, 30, 45 and 60 min and 2, 3, 6, 12 and 24 h after birth using the following measurements: physical examination (time between birth and sternal recumbency [T-SR]) heart rate (mHR); arterial blood gas analyses (arterial partial pressure in oxygen [PaO2], in carbon dioxide [PaCO2], arterial haemoglobin oxygen saturation [SaO(2)], alveolo-arterial difference in oxygen [AaDO(2)]); pulmonary function tests using the oesophageal balloon catheter technique (respiratory rate [RR], total pulmonary resistance [R-I], dynamic lung compliance [C-Ldyn], tidal volume [V-T] and minute Volume [V-E]; arterial and venous blood acid-base balance analyses (arterial and venous pH [pHa and pHv], bicarbonate concentration [HCO(3)a and HCO(3)v], base excess [BEa and BEv]); rectal temperature (RT); jugular venous blood sampling for, determination of metabolic variables (blood glucose [G], plasma lactate [L], serum cortisol [C], plasma noradrenaline [NA] and adrenaline [A] concentrations); haematological variables (red blood cell count [RBC], total haemoglobin concentration [Hb], Packed Cell Volume [PCV]) and passive immune transfer variables (total serum protein [TP] and beta(2)gammaglobulin [beta(2)gamma] concentrations). TECS significantly (Pless than or equal to0.05) influenced PaO2, SaO(2), AaDO(2), RR, V-T, V-E, C-Ldyn, RT, G, NA, A, RBC, Hb and TP: compared to TECSgreater than or equal to4 calves, TECSless than or equal to3 calves showed lower PaO2, SaO(2), V-T, C-Ldyn, RT, G, NA, A, RBC, Hb and TP and higher AaDO(2), RR, V-E. TECS differences progressively decreased and disappeared between 6 and 12 h after birth in TECS 2 and 3 calves but remained tip to 24 h in TECS 1 calves. Improved postnatal respiratory and metabolic adaptation in TECSgreater than or equal to4 calves were mainly related to differences in exposure to labour and subsequent hormonal surge: catecholamines, particularly A, enabled more effective removal of lung liquid and/or release of surfactant which contribute to better gas exchanges and induced greater energy mobilization to maintain adequate body temperature. It was concluded waiting for full cervical dilatation before performing CS should be encouraged because it promotes postnatal respiratory and metabolic adaptation in full-term BWB calves. [less ▲]

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See detailMass transfer by free and forced convection from single spherical liquid drops
Waheed, M Adekojo; Henschke, Martin; Pfennig, Andreas ULg

in International Journal of Heat & Mass Transfer (2002), 45(22), 4507-4514

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See detailLésions dentaires cervicales non carieuses - Première partie
Gueders, Audrey ULg

in Actualités Dentaires de l'Université de Liège (2002), 31

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See detailEffect of nutritional antioxidant supplementation on systemic and pulmonary antioxidant status, airway inflammation and lung function in heaves-affected horses
Kirschvink, Nathalie; Fievez, Laurence ULg; Bougnet, V. et al

in Equine Veterinary Journal (2002), 34

An oxidant/antioxidant imbalance in favour of oxidants has been identified as playing a decisive role in the pathogenesis of chronic inflammatory airway diseases. Nutritional antioxidant supplementation ... [more ▼]

An oxidant/antioxidant imbalance in favour of oxidants has been identified as playing a decisive role in the pathogenesis of chronic inflammatory airway diseases. Nutritional antioxidant supplementation might reduce oxidative damage by enhancement of the antioxidant defence, thereby modulating inflammatory processes. In a placebo-controlled, blind study, it was tested whether a dietary antioxidant supplement administered for 4 weeks would improve lung function and reduce airway inflammation in heaves-affected horses. Eight horses in clinical remission of heaves were investigated at rest and after a standardised exercise test before and after treatment with an antioxidant supplement (consisting of a mixture of natural antioxidants including vitamins E and C and selenium from a variety of sources) or placebo (oatfeed pellets without additive). Pulmonary function and exercise tolerance were monitored; systemic and pulmonary lining fluid uric acid, glutathione and 8-epi-PGF(2alpha) were analysed, and bronchoalveolar lavage (BAL) cytology and inflammatory scoring of the airways were performed. The antioxidant treatment significantly improved exercise tolerance and significantly reduced endoscopic inflammatory score. Plasma uric acid concentrations were significantly reduced, suggesting downregulation of the xanthine-dehydrogenase and xanthine-oxydase pathway. Haemolysate glutathione showed a nonsignificant trend to increase, while plasma 8-epi-PGF(2alpha) remained unchanged. Pulmonary markers and BAL cytology were not significantly affected by antioxidant supplementation. The present study suggests that the antioxidant supplement tested modulated oxidant/antioxidant balance and airway inflammation of heaves-affected horses [less ▲]

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See detailStratégies de prévention des avortements provoqués par les herpèsvirus et les pestivirus de ruminants
Thiry, Etienne ULg

in Annales de Médecine Vétérinaire (2002), 146(3, JUN-JUL), 161

Bovine herpesviruses 1 and 4 (BoHV-1 and BoHV-4) and caprine herpesvirus (CpHV-1) are responsible of abortion. Abortions caused by BoHV-1 happen during outbreaks of infectious bovine rhinotracheitis ... [more ▼]

Bovine herpesviruses 1 and 4 (BoHV-1 and BoHV-4) and caprine herpesvirus (CpHV-1) are responsible of abortion. Abortions caused by BoHV-1 happen during outbreaks of infectious bovine rhinotracheitis whereas BoHV-4 is associated with abortion but is not a very high risk factor. CpHV-1 infection is not yet identified in Belgium and in France. Bovine and ovine pestiviruses, bovine viral diarrhea and Border disease viruses, respectively, provoke abortions mainly during the first half of gestation. At the herd level, the prevention of abortion caused by these viruses can be achieved by the control of virus circulation with the help of vaccination or the elimination of persistently infected animals. At the individual level, immunisation with inactivated vaccines induces a high humoral response with high levels of neutralising antibodies, able to prevent the viremia and foetus infection. [less ▲]

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See detailCoalescence of secondary dispersions in fiber beds
Speth, Hauke; Pfennig, Andreas ULg; Chatterjee, Michael et al

in Separation and Purification Technology (2002), 29(2), 113-119

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See detailApproches pharmacologiques de prévention du diabète de type 2.
SCHEEN, André ULg; PAQUOT, Nicolas ULg; LETIEXHE, Michel ULg et al

in Médecine et Hygiène (2002), 60

L’augmentation rapide de la prévalence du diabète de type 2 impose la mise en place de stratégies de prévention. Outre les mesures hygiéno-diététiques, essentielles, diverses approches pharmacologiques ... [more ▼]

L’augmentation rapide de la prévalence du diabète de type 2 impose la mise en place de stratégies de prévention. Outre les mesures hygiéno-diététiques, essentielles, diverses approches pharmacologiques ont apporté récemment la preuve d’une certaine efficacité chez les sujets à risque de par la présence d’un excès pondéral et/ou d’une diminution de la tolérance au glucose. C’est le cas de plusieurs antidiabétiques oraux comme la metformine, l’acarbose ou encore la troglitazone. C’est également le cas de médicaments anti-obésité comme l’orlistat et, peut-être aussi, la sibutramine. L’inhibition du système rénine-angiotensine par un inhibiteur de l’enzyme de conversion ou par un antagoniste sélectif des récepteurs AT1 peut aussi, outre protéger contre les complications cardiovasculaires, prévenir l’apparition d’un diabète de type 2. Enfin, le rôle des médicaments hypolipidémiants reste controversé. De nouvelles études prospectives sont en cours pour confirmer ces résultats. [less ▲]

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See detailLes privatisations d'entreprises publiques en économie de marché: quelques observations juridiques
Thirion, Nicolas ULg

in Chroniques de Droit Public = Publiekrechtelijke Kronieken (2002), 2

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See detailUn moine est mort : sa vie commence.Anno 1048 obiit Poppo abbas Stabulensis.
George, Philippe ULg

in Moyen Age (Le) (2002), 108(3-4), 497-506

Poppo, the abbot at Stavelot-Malmedy died in the abbey of Marchiennes in Hainaut on 25 January 1048. The scene of the saint's death, which his hagiographer described in grand manner some ten years later ... [more ▼]

Poppo, the abbot at Stavelot-Malmedy died in the abbey of Marchiennes in Hainaut on 25 January 1048. The scene of the saint's death, which his hagiographer described in grand manner some ten years later, deserves to be examined closely, as indeed the staging of his funeral, this stepping onto the threshold of "rebirth" into eternal life and sainthood (dies natalis). The Vita Popponis follows the rules of the hagiographic genre, in which the monk's death is inevitably highlighted. [less ▲]

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