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See detailLe feuilleton de l'expertise (contradictoire) en matière pénale
Jacobs, Ann ULg

in Information et document juridqiques (2000), (4), 1-2

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See detailL'image du mois. Thrombose de la veine porte
Ancion, G.; Delwaide, Jean ULg; Boverie, Jacques ULg

in Revue Médicale de Liège (2000), 55(8), 761-762

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See detailA numerically efficient data analysis method with error map generation
Rixen, M.; Beckers, Jean-Marie ULg; Brankart, J. M. et al

in Ocean Modelling (2000), 2

The variational inverse model (VIM) for data analysis was already shown to be statistically equivalent to objective analysis (OA) provided the covariance function for the OA and the VIM reproducing kernel ... [more ▼]

The variational inverse model (VIM) for data analysis was already shown to be statistically equivalent to objective analysis (OA) provided the covariance function for the OA and the VIM reproducing kernel are identical. The VIM, however does not allow a direct derivation of the error field associated with the analysis. The purpose of the paper is to extend the one to one correspondance between the two analysis shemes by proposing a heuristic statistical error expression for the VIM. The numerical efficiency on analysis and error map generation of both methods is compared on quasi-synoptic and climatological data sets. It is shown that the VIM analysis and error map generation offers interesting numerical skills in both case studies. [less ▲]

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See detailSpecific phosphorylation of Torpedo 43 rapsyn by endogenous kinase(s) with thiamine triphosphate as the phosphate donor
Nghiêm, Hoang O; Bettendorff, Lucien ULg; Changeux, Jean-Pierre

in FASEB Journal (2000), 14

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See detailLes encéphalopathies à herpèsvirus bovins
Meyer, Gilles; D'Offay, Jean; Thiry, Etienne ULg

in Point Vétérinaire (2000), 31

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See detailReactions involving inorganic compounds
Krief, A.; Delmotte, C.; Colaux, Catherine ULg

in Pure & Applied Chemistry (2000), 72(9), 1709-1713

Selenium chemistry became, over the last 30 years, particularly useful for synthetic organic chemistry [1]. Inorganic as well as organic selenium compounds allow transformation which otherwise cannot be ... [more ▼]

Selenium chemistry became, over the last 30 years, particularly useful for synthetic organic chemistry [1]. Inorganic as well as organic selenium compounds allow transformation which otherwise cannot be done or require much more drastic conditions to proceed. We have over the last 25 years explored the reactivity of elemental selenium as well as its inorganic and organic derivatives. We report here our recent finding concerning (i) organic diselenols and -diselenolates and (ii) the role of selenoxides in the enantioselective dihydroxylation of C,C double bonds using catalytic amounts of osmium tetroxide. [less ▲]

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See detailDynamics of total lipids and fatty acids during embryogenesis and larval developpement of Eurasian perch (Perca fluviatlis)
Abi-Ayad, Amine; Kestemont, P.; Mélard, Charles ULg

in Fish Physiology and Biochemistry (2000), 23

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See detailLong-Term Protection Against Bovine Leukaemia Virus Replication In Cattle And Sheep
Kerkhofs, P.; GATOT, Jean-Stéphane ULg; Knapen, K. et al

in Journal of General Virology (2000), 81

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See detailL'infection de la chèvre par l'herpèsvirus caprin de type 1
Engels, M.; Thiry, Etienne ULg

in Point Vétérinaire (2000), 31

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See detailMass-murder deletion of 19 Orfs from Saccharomyces cerevisiae chromosome XI
Vandenbol, Micheline ULg; Fairhead, C.

in Gene (2000), 247(1-2),

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See detailLe rôle des voies ferrées dans la mise en place des reptiles en Belgique et dans quelques terroitoires adjacents (Nord et Nord-Est de la France, Grand-Duché de Luxembourg)
Graitson, Eric ULg; Hussin, José; Parent, Georges Henri

in Naturalistes Belges (Les) (2000), 81(4), 376-395

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See detailSpondylolyse bilatérale: intérêt de la tomoscintigraphie osseuse.
Blocklet, Didier; Seret, Alain ULg; Schoutens, André

in Revue Médicale de Bruxelles (2000), 21

Clinical case.

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See detailLes débuts de l'humanité dans le Prométhée enchaîné d'Eschyle
Renaud, Jean-Michel ULg; Wathelet, Paul ULg

in Uranie : Mythes et Littératures (2000), 9

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See detailComparaison de l'usage d'huiles d'origine végétale ou minérale pour la lubrification de chaines de tronconneuse.
Bieswal, Marc; Debouche, Charles ULg; van Belle, Laurent

in Silva Belgica (2000), 107(2), 18-21

Des études en laboratoire ont montré que les huiles végétales présentent un pouvoir lubrifiant supérieur aux huiles minérales dans le domaine de la lubrification limite (Kabuya 1995). Par ailleurs, des ... [more ▼]

Des études en laboratoire ont montré que les huiles végétales présentent un pouvoir lubrifiant supérieur aux huiles minérales dans le domaine de la lubrification limite (Kabuya 1995). Par ailleurs, des chiffres avancés dans la littérature ou par des fournisseurs de lubrifiants annoncent la possibilité de réduire la consommation de lubrifiant en passant à un lubrifiant à base d'huile végétale. Des chiffres de réduction d'usure sont aussi avancés par des constructeurs de tronçonneuses. L'essai décrit dans cet article n'a pas permis de confirmer ces réductions de consommation et de frottement. [less ▲]

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See detailMeeting report. Topics in ecology: structure and function of plants and ecosystems.
Bogaert, Jan ULg

in IALE Bulletin (2000), 18(6), 4

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See detailSynthesis and evaluation of new RuCl2(p-cymene)(ER2R') and (eta(1):eta(6)-phosphinoarene)RuCl2 complexes as ring-opening metathesis polymerization catalysts
Jan, Dominique; Delaude, Lionel ULg; Simal, François et al

in Journal of Organometallic Chemistry (2000), 606(1), 55-64

New RuCl2(p-cymene)(ER2R') complexes (E = P, As, Sb; R, R' = H, alkyl, arylalkyl) have been synthesized and used as catalyst precursors for the ring-opening metathesis polymerization (ROMP) of cyclooctene ... [more ▼]

New RuCl2(p-cymene)(ER2R') complexes (E = P, As, Sb; R, R' = H, alkyl, arylalkyl) have been synthesized and used as catalyst precursors for the ring-opening metathesis polymerization (ROMP) of cyclooctene, cyclopentene, and norbornene. When ER2R' was a phosphinoarene, the p-cymene ligand could be displaced upon heating and tethered (eta(1):eta(6)-phosphinoarene)RuCl2 complexes were obtained. Simple thermogravimetric analysis (TGA) of the complexes provided clear-cut indication on their potential catalytic activity in ROMP. (C) 2000 Elsevier Science S.A. All rights reserved. [less ▲]

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See detailSeparation of non-steroidal anti-inflammatory drugs by capillary electrophoresis using non-aqueous electrolyte.
Fillet, Marianne ULg; Bechet, Isabelle; Fotsing, Lucas ULg et al

in Biomedical Chromatography : BMC (2000), 14(1), 12-3

Separation of non-steroidal anti-inflammatory drugs by capillary electrophoresis using non-aqueous electrolyte.

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See detailJuvenile Nephropathy in a Boxer, a Rottweiler, a Collie and an Irish Wolfhound
Peeters, Dominique ULg; Clercx, Cécile ULg; Michiels, L. et al

in Australian Veterinary Journal (2000), 78(3), 162-5

Juvenile nephropathy was diagnosed in a Boxer, a Rottweiler, a Collie and an Irish Wolfhound dog, each presenting with signs compatible with chronic renal failure. The diagnosis in each case was based on ... [more ▼]

Juvenile nephropathy was diagnosed in a Boxer, a Rottweiler, a Collie and an Irish Wolfhound dog, each presenting with signs compatible with chronic renal failure. The diagnosis in each case was based on the presence of persistence of poorly differentiated tissue (immature glomeruli and/or tubules, persistent mesenchyme) on histopathologic examination. Although juvenile nephropathy has been reported in many breeds of dog, this is the first report of the condition in the Collie and the Irish Wolfhound and only the second description in the Boxer and the Rottweiler. The possibility of an inherited origin of the condition in these four breeds is at present unknown. [less ▲]

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See detailAmphipods of a stony beach in the maritime Antarctic
Jazdzewski, Krzystov; De Broyer, Claude; Pudlarz, M. et al

in Polish Archives for Hydrobiology (2000), 47

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See detailMonitoring des animaux vivants : exemple d’un échantillonnage pour la détection des PCBs et des dioxines chez les bovins de boucherie en Belgique
Saegerman, Claude ULg; Boelaert, F.; Van Vlanderen, I. et al

in Epidémiologie et Santé Animale (2000), 38

In February 1999, a poisoning episode broke out in several industrial poultry farms in Belgium. The source of this contamination was found to be a stock of recycled fat that had been delivered by a firm ... [more ▼]

In February 1999, a poisoning episode broke out in several industrial poultry farms in Belgium. The source of this contamination was found to be a stock of recycled fat that had been delivered by a firm to several compound feed producers, between 19 and 31 January 1999. A very good correlation was observed between dioxins (PCDFs and PCDDs) and PCBs. Consequently a contamination mainly by PCBs was hypothesized. This finding made it possible to detect contaminated animals and animal products by dosage of the PCBs, more specifically by the 7 congeners with numbers 28, 52, 101, 118, 138, 153 and 180. The advantages of the dosage of PCBs compared to dioxins were its rapid test protocol and the fact that a larger number of laboratories could participate. In the cattle sector, 409 herds (0,81 p. cent) were submitted to the risk of feed contamination. The methodology used to detect a PCBs/dioxins contamination in the Belgian cattle population that was not submitted to the risk, is presented. This population is directly or indirectly destined for human consumption. It consisted in the systematic sampling of all calve fattening stations and all lots of exported bovines, and in the random sampling of slaughter cattle. This methodology is compared to the approach described in directive 96/23/CE. [less ▲]

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See detailUse of a simple tin oxide sensor array to identify five malodours collected in the field
Romain, Anne-Claude ULg; Nicolas, Jacques ULg; Wiertz, Véronique et al

in Sensors and actuators. B, Chemical (2000), 62(1), 73-79

A laboratory-made malodour sensing system including 12 commercial tin oxide gas sensors (Figaro Engineering) is used to identify five typical sources of olfactive annoyance: printing houses, paint shop in ... [more ▼]

A laboratory-made malodour sensing system including 12 commercial tin oxide gas sensors (Figaro Engineering) is used to identify five typical sources of olfactive annoyance: printing houses, paint shop in a coachbuilding, wastewater treatment plant, urban waste composting facilities and rendering plant. In this work, all the samples are collected in the field from real malodours in uncontrollable conditions. The ability of the system to predict the origin of unknowns odoriferous samples is investigated. The test of various pre-processing data algorithms shows that the best classification results are obtained with a parameter free of the sensor base-line. The differences in sensor responses among the five odours are shown by icon plots and confirmed by principal component analysis, which highlights four representative clusters. Classification models calibrated by discriminant analysis and artificial neural network are validated on unknowns samples. Chemical relationships between the sensors and the classification results proves that the recognition is not fortuitous. In spite of the influence of environmental parameters, results demonstrate the ability of a simple system to detect and identify typical olfactive annoyances. [less ▲]

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See detailMR1750785,Radiation in enclosures, Mbiock, Aristide and Weber, Roman
Rochus, Pierre ULg

in Mathematical Reviews [=MR] (2000)

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See detailFeed restriction in young bulls alters the onset of puberty in relationship with plasma insulin-like growth factor-I (IGF-I) and IGF-binding proteins.
Renaville, Robert ULg; Van Eenaeme, Christian ULg; Breier, B. H. et al

in Domestic Animal Endocrinology (2000), 18(2), 165-76

The objectives of this study were to evaluate the effect of feed restriction and re-alimentation on the onset of puberty and IGF status in peripubertal male calves and to compare the radioimmunoassay (RIA ... [more ▼]

The objectives of this study were to evaluate the effect of feed restriction and re-alimentation on the onset of puberty and IGF status in peripubertal male calves and to compare the radioimmunoassay (RIA) and western ligand blotting (WLB) methods for bovine IGFBP-2. Twelve prepubertal 290 d-old Belgian Blue bulls (mean weight: +/- 290 kg) were randomly assigned in three groups: a control group (NG; n = 4) receiving a classic fattening diet to induce "normal" growth (1.48 kg/d), a feed restricted group (RG; n = 4) to obtain reduced growth (0.50 kg/d) and, a severely restricted group (SG; n = 4) to nearly stop growth (0.08 kg/d). The feed restriction period was maintained over a period of 114 d. After the period of differential feeding, all animals received the control feed regime over a period of 100 d. Blood samples were collected at fortnightly intervals. Circulating IGF-I was measured by RIA whereas plasma IGFBPs was evaluated by WLB; IGFBP-2 was additionally quantified by RIA procedure. At the beginning of the trial, IGF-I levels were low (<100 ng/ml) and similar in the three groups in accordance with prepubertal status. In the NG group, a progressive rise in IGF-I was observed from Day 42 to Day 142 whereas in the RG and SG groups, IGF-I levels did not change until the experimental restriction period ended. The delay of the rise in plasma IGF-I was longer for the SG group, IGF-I remained low until 2 wk after the end of the period of restricted feeding. Surprisingly, although differences were detected for IGF-I levels between the three groups, the IGFBP-2 and -3 data, evaluated by WLB could only discriminate between NG and SG group and not between NG and RG. However, by using a RIA method, an IGFBP-2 decrease was observed in the NG group coincident with increasing IGF-I levels. For both RG and SG groups, IGFBP-2 levels remained high throughout the feed restriction period whereas plasma IGFBP-2 levels declined upon feeding in both groups. During this feed restriction period, IGFBP-2 was significantly lower in NG than in RG or SG groups. Moreover, SG group animals had higher levels in plasma IGFBP-2 than RG animals. In conclusion, puberty is characterized by developmental changes in plasma IGF-I and IGFBPs that were altered by feed restriction. Moreover, RIA evaluation of plasma IGFBP-2 is able to better reflect group differences than WLB. [less ▲]

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See detailPeut-on aider Rosetta sans assassiner Mozart ?
Crahay, Marcel ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2000), (3-4), 196-199

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See detailThe afa-related gene cluster in necrotoxigenic and other Escherichia coli from animals belongs to the afa-8 variant
Gérardin, J.; Lalioui, L.; Jacquemin, E. et al

in Veterinary Microbiology (2000), 76

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See detailHigh Resolution Electron Energy Loss Spectroscopy Investigation of the Vibrational Structure and Order of an Octadecanethiol Monolayer on Gold Substrates
Duwez, Anne-Sophie ULg; Yu, Li-Ming; Riga, Joseph et al

in Journal of Physical Chemistry B (2000), 104

The vibrational spectra induced by electron beam from octadecanethiol monolayers adsorbed on gold are reported as a contribution to study in detail the structure of the layers, through an analysis of the ... [more ▼]

The vibrational spectra induced by electron beam from octadecanethiol monolayers adsorbed on gold are reported as a contribution to study in detail the structure of the layers, through an analysis of the interaction mechanisms between the electrons and these organic ultrathin films. To show the different spatial distribution of scattered electrons from dipole and impact interactions with the monolayer, angular distributions of elastic and inelastic scattered electrons were measured for various primary energies. The absolute electron scattering loss probabilities from both dipole and impact interactions were estimated for different vibrational modes. The study of these different mechanisms was first hampered by the poor order of the layers adsorbed on evaporated gold. It became therefore highly desirable to perform the experiments on a single-crystal substrate. Using Au(111) and Au(100) single crystals as substrates, it was possible to separate the spectral response from impact and dipole interacting electrons. A negative ion resonant behavior has been observed in the energy dependence of the electron scattering differential cross sections [less ▲]

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See detailDielectronic structure of 2l-1s Transitions of Multicharged Ions of Argon with nuclear charges Z = 10 - 17
Biémont, Emile ULg; Quinet, Pascal ULg; Faenov, A.Ya et al

in Physica Scripta (2000), 61

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See detailNeospora caninum, un nouvel agent abortif chez les bovins
Losson, Bertrand ULg; Bourdoiseau, G.

in Bulletin des Groupements Techniques Vétérinaires (2000), 7

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See detailAbout the Vacuum UV Photoabsorption Spectrum of Methyl Fluoride (CH3F): the fine structure and its vibrational analysis.
Locht, Robert ULg; Leyh, Bernard ULg; Hoxha, A. et al

in Chemical Physics (2000), 257

The vacuum UV photoabsorption spectrum of CH3F has been recorded between 7 and 24 eV and has been analyzed in detail. Broad and structureless peaks are observed over the entire photon energy range. They ... [more ▼]

The vacuum UV photoabsorption spectrum of CH3F has been recorded between 7 and 24 eV and has been analyzed in detail. Broad and structureless peaks are observed over the entire photon energy range. They are all assigned to transitions to Rydberg states, members of series converging to the 2e-1, 5a-1 and 1e-1 ionization limits. These features are underlying very long series of narrow and weak structures in the range of 10-13.2 eV. Through a continuum subtraction procedure, about 70 lines could clearly be identified. These have been assigned to long vibrational progressions belonging to Rydberg states corresponding to the 2e->3p and 2e->6s/5d configurations. These states are split by a Jahn-Teller distortion. Ab initio calculations lead to a Jahn-Teller stabilization energy of about 0.84 eV. The main features observed in the two bands are assigned to one single long vibrational progression of hcomega=0.160 eV (1290cm-1) related to the C-F Jahn-Teller inactive stretching vibration. The remaining structure is assigned to the low excitation of the Jahn-Teller active vibrational modes, i.e. the bending modes nu5 and nu6 characterized by hcomega5=0.120 eV (970 cm-1) and hcomega6=0.082 eV (660 cm-1). Leaning on the present results and assignments previously reported photoelectron spectroscopic data on CH3F+ [L. Karlsson, R. Jadrny, L. Mattsson, F.T. Chau, K. Siegbahn Phys.Scripta 16 (1977) 225] have been reconsidered and reinterpreted. [less ▲]

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See detailPhotoelectron Spectroscopy of Vinyl Bromide and Intramolecular Dynamics of the ionic B state.
Hoxha, A.; Locht, Robert ULg; Leyh, Bernard ULg et al

in Chemical Physics (2000), 256

In this paper we report the He(I) photoelectron spectrum (PES) and the threshold photoelectron spectrum (TPES) of C2H3Br. The fine structure in the first two ionic states in the He(I) spectrum is assigned ... [more ▼]

In this paper we report the He(I) photoelectron spectrum (PES) and the threshold photoelectron spectrum (TPES) of C2H3Br. The fine structure in the first two ionic states in the He(I) spectrum is assigned to progressions belonging partially to previously unobserved vibrational modes. The TPES has been measured between 9.0 and 25.0 eV, and the photon energy range of 9.8-12.0 eV has been investigated in more detail. Extensive calculations with the GAUSSIAN set of programs have been performed to help in the assignment of the observed features. Furthermore, a conical intersection between the A2A" and the B2A" states was found to take place along the C-Br stretching coordinate. Intramolecular dynamics of the B2A" state, initially prepared in the Franck-Condon region, was probed by the Fourier transform of the spectrum. The B2A" state is almost readily depleted, most probably due to a very effective internal cnversion through the conical intersection. [less ▲]

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See detailQuoi de neuf dans le domaine de l'obesite?
Scheen, André ULg

in Revue Médicale de Liège (2000), 55(7), 756-9

We will briefly comment on some interesting topics presented at the 10th European Congress on Obesity (ECO, Antwerp, Belgium, 24-27 May 2000). Special attention has been focused upon: 1) the multi ... [more ▼]

We will briefly comment on some interesting topics presented at the 10th European Congress on Obesity (ECO, Antwerp, Belgium, 24-27 May 2000). Special attention has been focused upon: 1) the multi-endocrine function of the adipocyte cell; 2) the role of gene-environment interactions; 3) the regulatory mechanisms of energy balance; 4) the neuroendocrine hypothesis for the development of visceral adiposity; 5) the numerous complications associated with obesity, more particularly type 2 diabetes; and, finally, 6) obesity prevention, especially in childhood, and new pharmacological perspectives for the treatment of obesity. [less ▲]

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See detailPart 9: Shrinkage for service and accident conditions
Schneider, Ulrich; Schwesinger, P.; Debicki, Gérard et al

in Materials and Structures (2000), 33

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See detailFactors controlling the distribution of diatoms and Phaeocystis in the Ross Sea
Goffart, Anne ULg; Catalano, Guilio; Hecq, Jean-Henri ULg

in Journal of Marine Systems (2000), 27

The spatial and temporal distributions of phytoplankton pigments were investigated in the western and south central Ross Sea during austral spring 1994 and summer 1990. Large gradients in biomass and ... [more ▼]

The spatial and temporal distributions of phytoplankton pigments were investigated in the western and south central Ross Sea during austral spring 1994 and summer 1990. Large gradients in biomass and phytoplankton community composition were observed both in the east-west and south-north directions, in relation to differences in water column structure and stability, which themselves depend on the processes of ice retreat within the different areas. Important are melting in the western Ross Sea, which induces strong stratification, and ice breakup and wind stress in the south central Ross Sea, which result in deep mixing and weakly stratified waters. In the western Ross Sea, the highest chl a concentrations observed in this study (129 - 358 mg m-2 in the upper 100 m) were tightly coupled to the stratified region of meltwater influence and were dominated by diatoms, as indicated by elevated fucoxanthin concentrations (89 - 239 mg m-2 in the upper 100 m). In the diatom bloom area, high levels of phaeophorbides a (maximum value of 192 mg . m-2 in the upper 100 m) indicated that the dominant grazers, identified as Limacina helecina and copepods (Hecq et al., 1992), transferred a sustained part of the diatom production to the herbivore trophic level. Synthesis of our data with published information suggests that the diatom bloom we observed in the western Ross Sea was dominated by the species Fragilariopsis curta, and occurs annually off the coast of Victoria Land from 72°30'S to 77°S within 100 to 250 km from the coastal ice edge. It is assumed to persist on the order of 2.5 - 3 months during summer. In the south central Ross Sea, which was characterized by a poorly or unstratified water column, moderate chl a concentrations (55 - 186 mg m-2 in the upper 100 m) were found in the polynya and in the ice edge area in early spring. 19'-hexanoyloxyfucoxanthin, a biomarker for Phaeocystis, was the major contributor to the total carotenoid abundance (29 - 132 mg m-2 in the upper 100 m), corroborating the hypothesis that Phaeocystis are well adapted to develop in a relatively mixed water column. At the northern limit of the polynya, a quite narrow (c. a. 30 km) diatom bloom (31 - 67 mg fucoxanthin m-2 in the upper 100 m) overlapped with the Phaeocystis bloom in the slightly stratified marginal ice zone. This was an area of increased grazing pressure by various types of herbivorous zooplankton, among which very large amounts of krill were observed. [less ▲]

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See detailParticipation d'élèves de l'enseignement secondaire à des activités sportives extrascolaires. Comparaison selon leur niveau d'habileté
Piéron, Maurice ULg; Ledent, Maryse ULg; Delfosse, Catherine ULg et al

in Revue de l'Education Physique (2000), 40(2), 61-72

Les auteurs font partie du service de Pédagogie des activités physiques et sportives de l’Université de Liège. Ils ont effectué une étude portant sur le traitement différencié des élèves par les ... [more ▼]

Les auteurs font partie du service de Pédagogie des activités physiques et sportives de l’Université de Liège. Ils ont effectué une étude portant sur le traitement différencié des élèves par les enseignements en éducation physique, subventionnée par la Communauté française de Belgique, Administration de l’Enseignement et de la Recherche Scientifique. [less ▲]

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See detailSystèmes d’information géographique répartis ou distribués
Donnay, Jean-Paul ULg

in AM/FM-GIS BELUX News (2000), 17

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See detailThe effect of pressure on DSr (plag/melt) and DCr (opx/melt): implications for anorthosite petrogenesis
Vander Auwera, Jacqueline ULg; Longhi, John; Duchesne, Jean-Clair ULg

in Earth and Planetary Science Letters (2000), 178

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See detailSucceeded plant regeneration from very immature embryos of Phaseolus vulgaris
Geerts, P.; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg

in Annual Report of the Bean Improvment Cooperative (2000), (43), 206-207

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See detailLa spatio-cartographie
Donnay, Jean-Paul ULg

in Bulletin de la Société Géographique de Liège (2000), 38(1),

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See detailp63 is a prostate basal cell marker and is required for prostate development.
Signoretti, Sabina; Waltregny, David ULg; Dilks, James et al

in American Journal of Pathology (2000), 157(6), 1769-75

The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives (DeltaNp63). p63 is expressed in the basal cells of many ... [more ▼]

The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives (DeltaNp63). p63 is expressed in the basal cells of many epithelial organs and its germline inactivation in the mouse results in agenesis of organs such as skin appendages and the breast. Here, we show that prostate basal cells, but not secretory or neuroendocrine cells, express p63. In addition, prostate basal cells in culture predominantly express the DeltaNp63alpha isotype. In contrast, p63 protein is not detected in human prostate adenocarcinomas. Finally, and most importantly, p63(-/-) mice do not develop the prostate. These results indicate that p63 is required for prostate development and support the hypothesis that basal cells represent and/or include prostate stem cells. Furthermore, our results show that p63 immunohistochemistry may be a valuable tool in the differential diagnosis of benign versus malignant prostatic lesions. [less ▲]

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See detailDeletion of the 5'-ABL region: a recurrent anomaly detected by fluorescence in situ hybridization in about 10% of Philadelphia-positive chronic myeloid leukaemia patients.
Herens, Christian ULg; Tassin, Françoise ULg; Lemaire, Véronique ULg et al

in British Journal of Haematology (2000), 110(1), 214-6

Inclusion of the BCR-ABL ES probe in routine cytogenetics led to the identification of a subgroup of Philadelphia positive (Ph+) chronic myeloid leukaemia patients characterized by a 5'-ABL deletion. This ... [more ▼]

Inclusion of the BCR-ABL ES probe in routine cytogenetics led to the identification of a subgroup of Philadelphia positive (Ph+) chronic myeloid leukaemia patients characterized by a 5'-ABL deletion. This anomaly was observed in 5/51 cases (9.8%). Cytological and clinical data suggest that the 5'-ABL deletion may be associated with dysplastic features of polymorphonuclear cells and metamyelocytes and a short chronic phase duration. [less ▲]

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See detailComment apprendre aux élèves à développer une démarche experte et réflexive de résolution de problèmes
Fagnant, Annick ULg; Demonty, Isabelle ULg; Lejong, Michèle ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2000), (3-4), 51-65

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See detailIndicators of erythrocyte formation and degradation in rats with either vitamin A or iron deficiency.
Roodenburg, A. J.; West, C. E.; Beguin, Yves ULg et al

in Journal of Nutritional Biochemistry (2000), 11(4), 223-30

Vitamin A deficiency produces anemia and altered iron status. In this study with rats we tested two hypotheses regarding vitamin A deficiency: (1) that it impairs erythropoiesis, leading to an increased ... [more ▼]

Vitamin A deficiency produces anemia and altered iron status. In this study with rats we tested two hypotheses regarding vitamin A deficiency: (1) that it impairs erythropoiesis, leading to an increased red cell turnover, and (2) that it inhibits the glycosylation of transferrin. Erythropoietic activity was assessed indirectly by determining the myeloid:erythroid ratio in bone marrow smears, the number of erythroid colonies in the red pulp of spleen, the blood reticulocyte index, and zinc protoporphyrin and plasma transferrin receptor concentrations. Transferrin glycosylation was assessed by measuring the sialic acid content of transferrin. The effects of vitamin A deficiency were compared with those of iron deficiency. Iron deficiency produced anemia and low iron levels in organs. Vitamin A deficiency produced low levels of plasma and hepatic retinol, and it induced decreased plasma total iron-binding capacity and raised iron levels in tibia and spleen. Short- but not long-term iron deficiency reduced the number of erythroid colonies in spleen; vitamin A deficiency had no influence. Neither iron nor vitamin A deficiency influenced the myeloid:erythroid ratio in bone marrow smears and the blood reticulocyte production. Plasma transferrin receptor and erythrocyte zinc protoporphyrin concentrations were not affected by vitamin A deficiency but increased with iron deficiency. Vitamin A deficiency did not stimulate erythrocyte breakdown, as indicated by unaltered plasma lactate dehydrogenase activity and reduced plasma total bilirubin levels. Both vitamin A and iron deficiencies raised the proportion of multiple sialylated transferrins in plasma. Thus, we have not found evidence that vitamin A deficiency affects erythropoiesis and erythrocyte turnover. The iron accumulation in spleen and bone marrow may be related to reduced iron transport due to inhibition of transferrin synthesis rather than inhibition of transferrin sialylation. [less ▲]

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See detailFootprint Analysis For Measurements Over A Heterogeneous Forest
Rannik, U.; Aubinet, Marc ULg; Kurbanmuradov, O. et al

in Boundary-Layer Meteorology (2000), 97(1),

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See detailNature et qualités de spatiocartes
Donnay, Jean-Paul ULg

in Bulletin de la Société Géographique de Liège (2000), 38

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See detailUse of sequential estimation of regressions and effects on regressions to solve large multitrait test-day models
Gengler, Nicolas ULg; Tijani, A.; Wiggans, G. R.

in Journal of Dairy Science (2000), 83(2), 3691-36918

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See detailOsmotic behaviour of in vitro produced bovine blastocysts in cryoprotectant solutions as a potential predictive test of survival
Kaidi, Safia; Donnay, I.; Lambert, Philippe ULg et al

in Cryobiology (2000), 41

The osmotic behavior of bovine blastocysts produced in vitro was filmed during exposure to and dilution of cryoprotectant solutions used for vitrification. The relationship between the changes in the ... [more ▼]

The osmotic behavior of bovine blastocysts produced in vitro was filmed during exposure to and dilution of cryoprotectant solutions used for vitrification. The relationship between the changes in the diameter of embryos and their subsequent survival was assessed. Embryos collected on Day 6 and Day 7 postinsemination were exposed to 10% glycerol (GLY) for 5 min, 10% GLY + 20% ethylene glycol (EG) for 5 min, and 25% Gly + 25% EG for 30 s, before dilution in 0.85 M galactose and finally in embryo transfer freezing medium (ETF). Embryos that had a higher probability of survival behaved as perfect osmometers, shrinking, reexpanding, or swelling according to an identical pattern, whereas embryos that deviated from this standard usually did not survive. The initial embryo diameter, duration of shrinkage and expansion in 10% glycerol, duration of reexpansion in ETF, and final embryo diameter were clearly predictive of the ability to hatch after culture in vitro. On a given day postinsemination, larger blastocysts were more likely than smaller blastocysts to survive and hatch after exposure to cryoprotectants with or without vitrification. [less ▲]

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See detailGiant lymph node hyperplasia.
NCHIMI LONGANG, Alain ULg; Biquet, J. F.; Rausin, L.

in JBR-BTR : organe de la Societe royale belge de radiologie (SRBR) = orgaan van de Koninklijke Belgische Vereniging voor Radiologie (KBVR) (2000), 83(1), 19

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See detailDeletion Analogues Of Transportan
Soomets, U.; Lindgren, M.; Gallet, X. et al

in Biochimica et Biophysica Acta-Biomembranes (2000), 1467(1), 165-76

Several shorter analogues of the cell penetrating peptide, transportan, have been synthesized in order to define the regions of the sequence, which are responsible for the membrane translocation property ... [more ▼]

Several shorter analogues of the cell penetrating peptide, transportan, have been synthesized in order to define the regions of the sequence, which are responsible for the membrane translocation property of the peptide. Penetration of the peptides into Bowes melanoma cells and the influence on GTPase activity in Rin m5F cellular membranes have been tested. The experimental data on cell penetration have been compared with molecular modeling of insertion of peptides into biological membranes. Omission of six amino acids from the N-terminus did not significantly impair the cell penetration of the peptide while deletions at the C-terminus or in the middle of the transportan sequence decreased or abolished the cellular uptake. Most transportan analogues exert an inhibitory effect on GTPase activity. Molecular modeling shows that insertion of the transportan analogues into the membrane differs for different peptides. Probably the length of the peptide as well as the location of aromatic and positively charged residues have major impact on the orientation of peptides in the membranes and thereby influence the cellular penetration. In summary, we have designed and characterized several novel short transportan analogues with similar cellular translocation properties to the parent peptide, but with reduced undesired cellular activity. [less ▲]

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See detailSimplification of boolean verification conditions
Gribomont, Pascal ULg

in Theoretical Computer Science (2000), 239(1), 165-185

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See detailEvaluation of Accuracy of Pulse Oximetry in Newborn Calves
Uystepruyst, Christophe; Coghe, Joost; Bureau, Fabrice ULg et al

in Veterinary Journal (2000), 159(1), 71-76

In human medicine, pulse oximetry is widely used to measure non-invasively and accurately the percentage of oxygen saturation of arterial haemoglobin (SpO(2)). Recently, pulse oximetry has been used in ... [more ▼]

In human medicine, pulse oximetry is widely used to measure non-invasively and accurately the percentage of oxygen saturation of arterial haemoglobin (SpO(2)). Recently, pulse oximetry has been used in calves, but its accuracy has not been evaluated in newborn calves. The purpose of this study was to evaluate the accuracy of a pulse oximeter in newborn calves by comparing SpO(2) with arterial oxyhaemoglobin saturation (SaO(2)) obtained by use of a blood gas analyser. Fifty-five newborn calves were investigated from birth to 20 days old. Pulse oximetry readings and arterial blood samples were performed 5, 15, 30, 45, 60 min, 2, 3, 6, 12, 24 h and 1 and 3 weeks after birth. The transmission-type sensors of the pulse oximeter were fixed at the recommended site in the bovine species (at the base of the calf tail, where the skin had been shaved and was not pigmented) and arterial blood samples were withdrawn from the subclavian artery and analysed for SaO(2). Five-hundred paired data of SaO(2) and mean SpO(2)(mSpO(2)) were collected. Linear regression of the pooled data indicated a highly significant correlation of mSpO(2) with SaO(2) (r = 0.87;P< 0.001; mSpO(2) = 15.8 + 0.84 SaO(2)). The overall data bias value was positive (+2.1%), which indicated that the pulse oximeter tended to overestimate the SaO(2). The bias value for each SaO(2) category tended to become higher for lower ranges of SaO(2). Precision was also lower when SaO(2) values were low. The lower the SaO(2) value, the higher the positive bias (overestimation) and the lower the precision. These results suggest that pulse oximetry provides a relatively accurate non-invasive, immediate and portable method to monitor SaO(2) and to evaluate objectively the pulmonary function effectiveness in newborn calves during their adaptation to extra-uterine life. [less ▲]

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See detailNovel functionalization routes of poly(ε-caprolactone)
Lecomte, Philippe ULg; Detrembleur, Christophe ULg; Lou, Xudong et al

in Macromolecular Symposia (2000), 157

The aluminum alkoxide mediated ring opening polymerization of functional lactones, such as γ-ethylene ketal-ε-caprolactone (TOSUO), γ-(triethylsilyloxy)-ε-caprolactone (SCL) and γ-bromo-ε-caprolactone ... [more ▼]

The aluminum alkoxide mediated ring opening polymerization of functional lactones, such as γ-ethylene ketal-ε-caprolactone (TOSUO), γ-(triethylsilyloxy)-ε-caprolactone (SCL) and γ-bromo-ε-caprolactone (γBrCL), is a versatile route to polyesters containing ketal, ketone, alcohol and bromide groups. As result of living polyaddition mechanism, random and block copolymerization of εCL and γBrCL has been successfully carried out. The reactivity ratios are quite similar (1.08 for ε-CL, and 1.12 for γBrCL). These random copolymers are semicrystalline when they contain less than 30 mol% of γBrCL, otherwise they are amorphous. No transesterification reaction occurs during the sequential polymerization of ε-CL and γBrCL leading to block copolymers. Reaction of poly(εCL-co-γBrCL) with pyridine provides quantitatively a polycationic polyester. Furthermore, the reaction of this random copolymer with l,8-diazabicyclo[5.4.0] undec-7-ene (DBU) is a route to unsaturated polyesters, whose the non conjugated double bonds can be quantitatively converted into epoxides by reaction with m-chloroperbenzoic acid (mCPBA). No chain degradation is detected during these derivatization reactions of poly (εCL-co-γBrCL). [less ▲]

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See detailModelaçao numérica do comportamento de uma estrutura metalica sujeita à acçao do fogo - Analise de varios cenarios de incëndio
Vila Real, P. M. M.; Piloto, P. A. G.; Franssen, Jean-Marc ULg

in Revista Portuguesa de Engenharia de Estruturas (RPEE) (2000), 47

In this work it has been analysed several possible fire scenarios in a steel structure of a commercial building, in order to find which one corresponds to the lowest fire resistance of the structure. It ... [more ▼]

In this work it has been analysed several possible fire scenarios in a steel structure of a commercial building, in order to find which one corresponds to the lowest fire resistance of the structure. It has been used a geometrically and materially non-linear finite element program specially established for the analysis of structures submitted to the fire. As the analysed structure doesn't reach the fire resistance suggested by the Portuguese standards, some solutions are proposed to increase its fire resistance. [less ▲]

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See detailOnze ans de responsabilité médicale pénale sur le ressort de la Cour d'appel de Liège du 01.01.1985 au 31.12.1995
Boxho, Philippe ULg

in Journal de Médecine Légale, Droit Médical (2000), 78/43

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See detailCompte-rendu de Sandra Laugier, Quelle philosophie du langage aujourd'hui ?
Leclercq, Bruno ULg

in Recherches Husserliennes (2000), 13

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See detailPharma-clinics. Le medicament du mois. Le rofecoxib (Vioxx).
Scheen, André ULg

in Revue Médicale de Liège (2000), 55(7), 751-3

Rofecoxib (Vioxx, Merck Sharp & Dohme) is a potent and selective inhibitor of the COX-2 isoform of cyclooxygenase which is used as a nonsteroidal anti-inflammatory drug (NSAID). It is indicated in the ... [more ▼]

Rofecoxib (Vioxx, Merck Sharp & Dohme) is a potent and selective inhibitor of the COX-2 isoform of cyclooxygenase which is used as a nonsteroidal anti-inflammatory drug (NSAID). It is indicated in the symptomatic relief of pain due to osteoarthritis. The initial oral dosage of rofecoxib is 12.5 mg once daily in adults, and this dose may be increased up to a maximal dosage of 25 mg once daily if necessary. Its clinical efficacy seems to be similar to that of other NSAIDs at maximal recommended dosages, but its safety profile, especially gastrointestinal tolerance, is much better because of the COX-2 selectivity. Ongoing clinical trials are performed in patients with rheumatoid arthritis. [less ▲]

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See detailEffect of pH on CN-resistant respiratory activity and regulation on Vigna uniguiculata mitochondria
Lima-junior, A.; Fernandes de melo, D.; Costa, J. H. et al

in Plant Physiology & Biochemistry (2000), 38

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See detailEvolution des connaissances sur la membrane du globule gras du lait: synthese bibliographique.
Danthine, Sabine ULg; Blecker, Christophe ULg; Paquot, Michel ULg et al

in Lait (2000), 80(2), 209-222

The milk fat globule is essentially an oil droplet enclosed in a protective 'membrane', partially derived from the plasma membrane of the lactating cell, and which assures the dispersion of the milk fat ... [more ▼]

The milk fat globule is essentially an oil droplet enclosed in a protective 'membrane', partially derived from the plasma membrane of the lactating cell, and which assures the dispersion of the milk fat in the milk plasma. This protective coat is generally called 'the milk fat globule membrane' (MFGM). This review examines the present state of knowledge on the MFGM. The topics dealt with are: origin and formation, isolation From milk, composition (lipids and proteins), structure (a new model is proposed), functional properties and the role that the MFGM plays in protection against lipolysis. [less ▲]

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See detailSpectroscopic properties of protochlorophyllide analyzed in situ in the course of etiolation and in illuminated leaves
Schoefs, B.; Bertrand, M.; Franck, Fabrice ULg

in Photochemistry & Photobiology (2000), 72(1), 85-93

The spectroscopic properties of photoactive (ie. flash-transformable) and nonphotoactive protochlorophyll(ide)s (Pchl(ide)) were reinvestigated during the development of bean leaves in darkness. Two ... [more ▼]

The spectroscopic properties of photoactive (ie. flash-transformable) and nonphotoactive protochlorophyll(ide)s (Pchl(ide)) were reinvestigated during the development of bean leaves in darkness. Two phases in the process of Pchl(ide) accumulation were apparent from quantitative measurements of pigment content: a lag phase (first week) during which photoactive Pchl(ide) accumulated faster than nonphotoactive Pchl(ide); and a fast phase (second week), showing parallel accumulation of both types of Pchl(ide). 'Flashed-minus-dark' absorbance difference spectra recorded in situ at 77 K showed that P650-655 was the predominant form of photoactive protochlorophyllide regardless of developmental stage. Quantitative analysis of energy migration processes between the Pchl(ide) forms showed the existence of energy transfer units containing a 1:8 ratio of nonphotoactive and photoactive Pchl(ide)s during development. Gaussian deconvolution of in situ 77 K fluorescence spectra indicated that the 633 nm band of nonphotoactive Pchl(ide) was made of four bands, at 625, 631, 637 and 643 nm, whose relative amplitudes only slightly changed during development. The emission band of photoactive Pchlide was also analyzed using the same method. Three components were found at 644, 652 and 657 nm, The emission band of P650-655 included the last two components, which become predominant only in fully etiolated plants. Photoactive Pchlide with an emission maximum at 653 nm was detected in the light during development of leaves of photoperiodically grown plants. [less ▲]

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See detailClinical course and predictive factors for cyclosporin-induced autologous graft-versus-host disease after autologous haematopoietic stem cell transplantation.
Baron, Frédéric ULg; Gothot, André ULg; Salmon, Jean ULg et al

in British Journal of Haematology (2000), 111(3), 745-53

The administration of cyclosporin A (CyA) after autologous haematopoietic stem cell transplantation (HSCT) induces a systemic autoimmune syndrome mimicking graft-vs.-host disease (GVHD). This syndrome ... [more ▼]

The administration of cyclosporin A (CyA) after autologous haematopoietic stem cell transplantation (HSCT) induces a systemic autoimmune syndrome mimicking graft-vs.-host disease (GVHD). This syndrome, termed autologous GVHD has notable anti-tumour activity in animal studies. We intended to induce autologous GVHD with CyA in patients undergoing an autologous HSCT. We prospectively studied 118 patients with miscellaneous malignancies undergoing an autologous HSCT with low-dose CyA to characterize the clinical syndrome, its frequency and clinical course, and to determine the factors affecting its incidence. Patients received CyA from d -1 through to d 28, first starting at 2 mg/kg intravenously and then orally as soon as feasible. The dose was adjusted to achieve pre-dose blood levels around 100 ng/ml. A skin biopsy was performed when a skin rash was observed. Thirty-three percent of the patients developed clinical GVHD: clinical stage 1 in 21 patients, stage 2 in seven patients, and stage 3 in three patients. Although total body irradiation (TBI) or high-dose cyclophosphamide were previously thought to be needed, autologous GVHD occurred in five out of 12 patients (42%) after a preparative regimen with high-dose melphalan alone. Autologous GVHD was significantly more frequent in patients older than 33 years, in patients who had received high doses of granulocyte-macrophage colony forming units (CFU-GM) and in those with a diagnosis of myeloid malignancy, compared with those with lymphoid malignancies or solid tumours. A significant negative association was also found with HLA-DR6. In lymphoma patients, GVHD occurred more frequently in advanced disease than in first or second complete remission (CR1-2) patients. All other factors studied were not predictive for GVHD. In conclusion, CyA-induced GVHD is reproducibly and safely induced with doses of CyA adapted to achieve blood levels around 100 ng/ml. In retrospective analysis, there was no survival advantage for patients with GVHD. Phase III trials with this approach are needed to evaluate its anti-tumoral effect. [less ▲]

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See detailBinding and release of metallic cations by a polyaniline-based resin
Jérôme, Christine ULg; Martinot, Lucien; Jérôme, Robert ULg

in Radiochimica Acta (2000), 88(1), 39-46

The electrochemical control of the binding of metallic ions (actinides and a lanthanide) by redox active polyaniline resin, i.e., polyaniline doped by polystyrene sulfonate (Pani/PSSO3), has been studied ... [more ▼]

The electrochemical control of the binding of metallic ions (actinides and a lanthanide) by redox active polyaniline resin, i.e., polyaniline doped by polystyrene sulfonate (Pani/PSSO3), has been studied. Electrochemical and nuclear techniques (cyclic voltammetry, α -counting, Rutherford backscattering) have been used in order to characterize the exchange of thorium, lanthanum and uranium cations, particularly in terms of reversibility and efficiency. This electrochemical process has been compared with the chemical one promoted merely by pH variation, and the efficiency of the two methods has been discussed for each type of cation. [less ▲]

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See detailEvidence For Specific And Non-Covalent Binding Of Lipids To Natural And Recombinant Mycobacterium Bovis Bcg Hsp60 Proteins, And To The Escherichia Coli Homologue Groel
De Bruyn, J.; Soetaert, K.; Buyssens, P. et al

in Microbiology-Sgm (2000), 146

Heat-shock proteins (Hsps) from various origins are known to share a conserved structure and are assumed to be key partners in the biogenesis of proteins. Fractionation of the mycobacterial Hsp60, a 65 ... [more ▼]

Heat-shock proteins (Hsps) from various origins are known to share a conserved structure and are assumed to be key partners in the biogenesis of proteins. Fractionation of the mycobacterial Hsp60, a 65 kDa protein also called Cpn60, from Mycobacterium bovis BCG zinc-deficient culture filtrate on phenyl-Sepharose followed by Western blotting revealed the existence of four Hsp60-1 and Hsp60-2 forms, based on their hydrophobicity behaviour. Hsp60-2 species were further purified by ion-exchange chromatography and partial amino acid sequences of cyanogen bromide (CNBr) peptides of purified Hsp60-2 species showed identity with the amino acid sequence deduced from the hsp60-2 gene, indicating that the various Hsp60-2 forms are encoded by the same gene. In addition, the mycobacterial Hsp60-2 was overexpressed in E. coli using the pRR3Hsp60-2 plasmid and analysed on phenyl-Sepharose. The elution pattern of the recombinant Hsp60-2, as well as that of Escherichia coli GroEL, was similar to that of the native Hsp60-2 from the culture filtrate of M. bovis BCG and entirely different from that of the mycobacterial antigen 85. Extraction of mycobacterial Hsp60-2 forms, recombinant BCG Hsp60-2 and E. coli GroEL with organic solvents releases various amounts of non-covalently bound lipids. The presence of lipids on Hsp60-2 was confirmed by labelling M. bovis BCG with radioactive palmitate. The radioactivity was specifically associated with Hsp60 in the aqueous phase and the 19 and 38 kDa lipoproteins in the Triton X-114 phase. Analysis of the lipids extracted from purified Hsp60-2, recombinant BCG Hsp60-2 and E. coli GroEL by TLC showed the same pattern for all the samples. Acid methanolysis of the lipids followed by GC analysis led to the identification of C(16:0), C(18:0) and C(18:1) as the major fatty acyl constituents, and of methylglycoside in these proteins. Altogether, these data demonstrate that lipids are non-covalently bound to Hsp60-2 and homologous proteins. [less ▲]

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See detailLa réforme grégorienne et les tentatives de réforme du calendrier grégorien
Biémont, Emile ULg

in Bulletin de liaison du Département d'Etudes grecques, latines et Orientales de l'Université de Louvain (2000), 11

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See detailL'icône du visible
Steinmetz, Rudy ULg

in Etudes Phénoménologiques (2000)

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See detailOral contraception and cardiovascular risk factors during adolescence
Paulus, Dominique; Saint-Remy, Annie ULg; JeanJean, Michel

in Contraception (2000)

The objective of the present study was to analyze the pattern of oral contraceptive (OC) use in teenagers and to examine the relationship between OC use and other cardiovascular risk factors. The study ... [more ▼]

The objective of the present study was to analyze the pattern of oral contraceptive (OC) use in teenagers and to examine the relationship between OC use and other cardiovascular risk factors. The study was conducted in 24 Belgian secondary schools. Most students (1526 adolecents aged 12-17 years) agreed to participate (participation rate: 83.6%). Smoking, physical activity habits, menarche, and OC use were assessed by a self-administered questionnaire. Total cholesterol level, blood pressure and anthropometric measurements were also measured. Fourteen per cent of mature girls (14%, n=92) were OC users. Two-thirds of them (66.3%, n=61) were taking OC which contained either gestodene or desogestrel. Blood pressure and BMI were similar for OC users and non-users. Total cholesterol level was significantly higher in OC users than in non-users (191 mg/dL versus 172 mg/dL). Logistic regression model confirmed the significant influence of OC use on total cholesterol level (OR=3.08). OC users were also often smokers (39% versus 20% for non-users). In conclusion, the present study has found significant relationship between OC use and cardiovascular risk factors i.e., high total cholesterol and smoking. The first implication is a need for further research on lipoprotein profile in young OC users. Secondly, the combined use of OC and smoking in teenagers calls for preventive actions. [less ▲]

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See detailEffects of Mild Hypothermic Cardiopulmonary Bypass on Eeg Bispectral Index
Dewandre, Pierre-Yves; Hans, Pol ULg; Bonhomme, Vincent ULg et al

in Acta Anaesthesiologica Belgica (2000), 51(3), 187-90

We studied the effect of mild hypothermic cardiopulmonary bypass (30 degrees C) on the EEG Bispectral Index in 10 patients undergoing elective CABG. BIS was recorded at 11 event-related time points during ... [more ▼]

We studied the effect of mild hypothermic cardiopulmonary bypass (30 degrees C) on the EEG Bispectral Index in 10 patients undergoing elective CABG. BIS was recorded at 11 event-related time points during the procedure. After a significant decrease at the induction of anaesthesia, BIS was not further modified during the procedure. BIS was neither affected by surgical stimulation nor by CPB and mild hypothermia. We conclude that we did not find any reason to preclude the use of BIS to assess the hypnotic effects of anaesthetics during normothermic or mild hypothermic CPB. [less ▲]

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See detailThermal unfolding of an intermediate is associated with non-arrhenius kinetics in the folding of hen lysozyme
Matagne, André ULg; Jamin, M.; Chung, E. W. et al

in Journal of Molecular Biology (2000), 297(1), 193-210

A variety of techniques, including quenched-flow hydrogen exchange labelling monitored by electrospray ionization mass spectrometry, and stopped-flow absorbance, fluorescence and circular dichroism ... [more ▼]

A variety of techniques, including quenched-flow hydrogen exchange labelling monitored by electrospray ionization mass spectrometry, and stopped-flow absorbance, fluorescence and circular dichroism spectroscopy, has been used to investigate the refolding kinetics of hen lysozyme over a temperature range from 2 degrees C to 50 degrees C. Simple Arrhenius behaviour is not observed, and although the overall rate of folding increases from 2 to 40 degrees C, it decreases above 40 degrees C. In addition, the transient intermediate on the major folding pathway at 20 degrees C, in which the alpha-domain is persistently structured in the absence of a stable beta-domain, is thermally unfolded in a sigmoidal transition (T-m approximate to 40 degrees C) indicative of a cooperatively folded state. At all temperatures, however, there is evidence for fast (similar to 25%) and slow (similar to 75%) populations of refolding molecules. By using transition state theory, the kinetic data from various experiments were jointly fitted to a sequential three-state model for the slow folding pathway. Together with previous findings, these results indicate that the alpha-domain intermediate is a productive species on the folding route between the denatured and native states, and which accumulates as a consequence of its intrinsic stability. Our analysis suggests that the temperature dependence of the rate constant for lysozyme folding depends on both the total change in the heat capacity between the ground and transition states (the dominant factor at low temperatures) and the heat-induced destabilization of the alpha-domain intermediate (the dominant factor at high temperatures). Destablization of such kinetically competent intermediate species is Likely to be a determining factor in the non-Arrhenius temperature dependence of the folding rate of those proteins for which one or more intermediates are populated. (C) 2000 Academic Press. [less ▲]

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See detailCompte-rendu de l'ouvrage de Claudine Poulouin : Le temps des origines
Donneau, Olivier ULg

in Hieros : Bulletin Annuel de la Société Belgo-luxembourgeoise d'Histoire des Religions (2000), 4

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See detailA quantitative study of peripheral blood stem cell contamination in diffuse large-cell non-Hodgkin's lymphoma: one-half of patients significantly mobilize malignant cells.
Jacquy, C.; Soree, A.; Lambert, Frédéric ULg et al

in British Journal of Haematology (2000), 110(3), 631-7

Autologous transplantation using peripheral blood stem cells (PBSCs) collected after chemotherapy, followed by growth factor administration (ASCT), is increasingly used in the treatment of non-Hodgkin's ... [more ▼]

Autologous transplantation using peripheral blood stem cells (PBSCs) collected after chemotherapy, followed by growth factor administration (ASCT), is increasingly used in the treatment of non-Hodgkin's lymphoma (NHL). However, quantitative data regarding contaminating malignant cells in the harvests are still scarce. We prospectively investigated 37 diffuse large-cell lymphomas (DLCLs) in complete remission (CR) that were treated according to multicentric protocols at our centre. DNA was extracted from the diagnostic lymph node. The complementarity-determining region (CDR) III was sequenced and a patient-specific oligomer synthesized. Contamination was evaluated semiquantitatively by polymerase chain reaction (PCR) and was confirmed by a limiting dilution analysis. PBSCs collected at regeneration after administration of granulocyte colony-stimulating factor (G-CSF), steady-state bone marrow (BM) and peripheral blood samples at CR were compared. DNA was available in 37 patients, from which 22 rearrangements could be sequenced. Patients (n = 15) who had both the required follow-up samples and a suitable clonal marker were investigated. In two cases, the patient-specific PCR assay set up at diagnosis later gave false-negative results in samples in which clonal DNA was still detectable by other sets of primers. PBSC contamination was highly variable: 7 out of 15 patients showed a PBSC/BM ratio of NHL cells greater than 1 log, whereas 8 out of 15 patients showed no difference and could vary from one apheresis to another. Eight ASCTs were performed, five of which used highly contaminated PBSCs: four patients relapsed early, three with disseminated lymphoma. Thus, 50% of DLCLs in CR seem to mobilize significantly malignant cells at regeneration under G-CSF. Considering the higher numbers of cells reinfused, this translates into a much higher number of lymphoma cells reinfused when compared with autologous bone marrow transplantation (ABMT). However, their clonogenic potential remains unknown and, despite concerning observations in certain cases, it is still unclear whether this has an impact upon the outcome of ASCT. [less ▲]

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See detailBulletin bibliographique de la Société Rencesvals, 1999-2000
Horrent, Jacques ULg; Henrard, Nadine ULg

in Bulletin bibliographique de la Société Rencesvals (2000), (31), 208

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See detailJeux vidéos : enjeux et leurres
Leclercq, Dieudonné ULg; Denis, Brigitte ULg

in Education-Formation (2000)

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See detailFrom neurophysiology to genetics: cortical information processing in migraine underlies familial influences--a novel approach
Sandor, P. S.; Afra, J.; Proietti Cecchini, A. P. et al

in Functional Neurology (2000), 15(Suppl 3), 68-72

Migraine patients show impaired cortical information processing between attacks with deficient habituation of pattern-reversal visual evoked potentials (VEP), and strong intensity dependence of auditory ... [more ▼]

Migraine patients show impaired cortical information processing between attacks with deficient habituation of pattern-reversal visual evoked potentials (VEP), and strong intensity dependence of auditory cortical evoked potentials (IDAP). This could be a genetic trait as certain genetic patterns are known for evoked potentials in healthy subjects. VEP-habituation and IDAP were studied in 40 migraine patients, i.e. pairs of 20 parents and their children. We developed a novel approach based on Monte Carlo statistics to selectively assess vertical familial influences. Both groups, parents and children, were characterized by abnormal VEP-habituation and IDAP. However, similarity between related pairs was far more pronounced than similarity between unrelated pairs. Assessed with a novel statistical approach, familial influences proved to be highly significant in determining cortical information processing in migraineurs, thus supporting the important role of genetic factors. [less ▲]

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See detailLes évaluations externes en Communauté française de Belgique : quel sens donner à la mesure ?
Lafontaine, Dominique ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2000), (1-2), 110-114

Quelles sont les origines et les modalités du dispositif d'évaluation externe mis en place en Communauté française de Belgique à partir de 1994-1995 ? Quelle(s) fonction(s) est-il supposé remplir ? L ... [more ▼]

Quelles sont les origines et les modalités du dispositif d'évaluation externe mis en place en Communauté française de Belgique à partir de 1994-1995 ? Quelle(s) fonction(s) est-il supposé remplir ? L'analyse met en évidence le paradoxe d'un dispositif institutionnel rejeté à la marge du cadre législatif et dont la définition des finalités est laissée à l'appréciation des acteurs appelés à le mettre en oeuvre. [less ▲]

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See detailLa sécurité des filières agro-alimentaires : de l'éthique à l'étiquette.
Sindic, Marianne ULg

in Cahiers Agricultures (2000), 9

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See detailDiscovery en de WHPC (case note : Commercial Court of Termonde - 3 January 2000
Wautelet, Patrick ULg

in Revue de Droit Commercial Belge [= RDC] = Tijdschrift voor Belgisch Handelsrecht [=TBH] (2000)

In this short comment on a decision issued by the Commercial Court of Termonde, I offer some thoughts on the possibility for a business established in Belgium to seek protection in Belgium against ... [more ▼]

In this short comment on a decision issued by the Commercial Court of Termonde, I offer some thoughts on the possibility for a business established in Belgium to seek protection in Belgium against discovery proceedings in which it is involved before a court in the US. In the case commented, the court refused the relief sought mainly because it found that enjoining the defendant would constitute undue influence in the proceedings pending in the US. [less ▲]

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See detailTraitement des cephalees de tension
Schoenen, Jean ULg

in Revue Neurologique (2000), 156(Suppl 4), 487-92

The scientific basis of tension- type headache suffers from the lack of precise pathophysiological knowledge and the heterogenecity of this disorder. Treatment of acute tension-type headache episodes is ... [more ▼]

The scientific basis of tension- type headache suffers from the lack of precise pathophysiological knowledge and the heterogenecity of this disorder. Treatment of acute tension-type headache episodes is more effective with an NSAIDs (ibuprofen 400-800mg, naproxen 550-825mg, ketoprofen 50-75mg) than with aspirin or paracetamol. Caffein containing preparations of NSAIDs are slightly superior, but should not be taken frequently to avoid headache chronification. For chronic tension-type headache, relaxation therapies with EMG biofeedback and tricyclics have about the same efficacy rate of 40-50p.100. Physical therapy and acupuncture are in general less effective. There is thus clearly a need for better strategies, e.g. combination of available therapies and novel approaches. [less ▲]

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See detailÀ la recherche du Candide liégeois
Droixhe, Daniel ULg

in Australian Journal of French Studies (2000), 37(2), 127-164

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See detailDetermination Of The Methionine Requirement Of Growing Double-Muscled Belgian Blue Bulls With A Three-Step Method
Froidmont, Eric; Beckers, Yves ULg; Thewis, André ULg

in Journal of Animal Science (2000), 78(1), 233-241

The three-step technique was used to determine the requirements of total amino acids (TAA) and the first-limiting amino acid (AA) in growing double- muscled Belgian Blue bulls (BBb). In Exp. 1, three ... [more ▼]

The three-step technique was used to determine the requirements of total amino acids (TAA) and the first-limiting amino acid (AA) in growing double- muscled Belgian Blue bulls (BBb). In Exp. 1, three double-muscled BBb weighing initially 306 ± 28 kg received a basal diet consisting of 30% meadow hay and 70% concentrate that was poor in digestible protein but had adequate NE because of continuous infusion of dextrose into the duodenum. The intestinal apparent digestibility of essential AA (EAA) was defined according to their duodenal and ileal flows. It averaged 72% but varied between 60% for Met and 79% for Arg. In Exp. 2, five double-muscled BBb (334 ± 22 kg) received the same diet supplemented with duodenal infusions of dextrose and four doses of Na-caseinate (28, 56, 84, and 112% of intestinal digestible dietary AA) in a 4 × 4 Latin square design with one additional animal. Nitrogen retention for the basal diet alone and the four increasing supplements of Na-caseinate reached 49, 61, 70, 80, and 86 g/d, respectively. Nitrogen utilization improved from 34.3% without Na-caseinate supplementation to a maximum of 40.6%, with the third dose supplying 788 g/d of apparently digestible AA. Based on patterns of plasma concentrations, Met, Phe, and Arg were probably the limiting AA when animals optimized N utilization. In Exp. 3, six double-muscled BBb (315 ± 25 kg) fed the basal diet received duodenal infusions of dextrose and AA, equivalent to the third dose in Exp. 2, except for digestible Met (9.3, 14.4, 18.4, 22.4, 26.4, and 30.4 g/d) in a 6 × 6 Latin square design. The Met requirement was close to 26.4 g/d on the basis of N retention. [less ▲]

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See detailThe Efficacy of Two Vaccination Schemes against Experimental Infection with a Virulent Amyxomatous or a Virulent Nodular Myxoma Virus Strain
Marlier, Didier ULg; Mainil, Jacques ULg; Boucraut-Baralon, C. et al

in Journal of Comparative Pathology (2000), 122(2-3, Feb-Apr), 115-22

Two types of myxomatosis vaccine are available commercially, namely, vaccine prepared from the Shope fibroma virus (SFV) and that prepared from an attenuated myxoma virus (MV) strain, e.gSG33. An ... [more ▼]

Two types of myxomatosis vaccine are available commercially, namely, vaccine prepared from the Shope fibroma virus (SFV) and that prepared from an attenuated myxoma virus (MV) strain, e.gSG33. An experiment was designed to compare two vaccination schemes for their ability to protect rabbits against challenge with either a virulent amyxomatous MV strain or a virulent nodular MV strain. Apart from a difference in the cutaneous expression of the disease, the two challenge strains resembled each other in respect of mortality rate, naso-conjunctival shedding of virus, and tissue infection. Vaccination with SFV alone failed to prevent clinical signs, naso-conjunctival shedding or tissue infection. Vaccination with SFV followed by a booster inoculation with SG33 protected rabbits against the development of clinical signs and significantly reduced both viral shedding in naso-conjunctival exudates and viral infection of eyelids, lungs and testes; virus was, however, isolated from testes of some surviving animals. [less ▲]

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See detailLa lecture, un ouvrage féminin: Enquête sur les pratiques de lecture auprès des jeunes de14-15 ans en Communauté française de Belgique
Lafontaine, Dominique ULg; Schillings, Patricia ULg

in Enjeux : Revue de Formation Continuée et de Didactique du Français (2000), 47-48

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See detailThe relationships between episodic memory, working memory, and executive functions: The contribution of the prefrontal cortex
Van der Linden, Martial ULg; Meulemans, Thierry ULg; Marczewski, Philippe et al

in Psychologica Belgica (2000), 40(4), 275-297

Discusses the neurophysiological functions of the prefrontal cortex related to memory processes. Functional brain imaging studies have revealed that distinct regions of the prefrontal cortex are engaged ... [more ▼]

Discusses the neurophysiological functions of the prefrontal cortex related to memory processes. Functional brain imaging studies have revealed that distinct regions of the prefrontal cortex are engaged in episodic memory operations, with some regions apparently part of multiple networks subserving different memory abilities. The prefrontal cortex is also involved in shifting processes. Working memory processes within the dorsolateral and ventrolateral frontal cortex are organized according to the type of processing required rather than the nature of the information being processed. The binding of contextual information, both incidentally and intentionally, is the most crucial process involved in episodic memory. Inhibition control involves not only bilateral prefrontal areas, but also posterior regions and subcortical structures, but the specific role of the cerebral areas is not clear at present. [less ▲]

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See detailEnzyme-Histochemical Detection of a Chymase-Like Proteinase within Bovine Mucosal and Connective Tissue Mast Cells
Jolly, Sandra ULg; Detilleux, Johann ULg; Coignoul, Freddy ULg et al

in Journal of Comparative Pathology (2000), 122(Feb-Apr), 155-162

The presence of chymase-like proteinase in bovine mast cells was investigated by an enzyme-histochemical technique (naphthol-AS-D-chloroacetate as substrate) in normal skin, primary bronchus, lung and ... [more ▼]

The presence of chymase-like proteinase in bovine mast cells was investigated by an enzyme-histochemical technique (naphthol-AS-D-chloroacetate as substrate) in normal skin, primary bronchus, lung and duodenum. The counts and distribution of chymase-positive and toluidine blue-positive mast cells were compared by means of successive staining. Mast cells with chymase-like activity were detected in all areas, but their proportion was greater in connective than mucosal tissues, with the exception of the skin. These results contrast with those obtained in rodents, in which chymase-like proteinases are detected in all tissues and also in all mast cells. Bovine mast cells are closer to those of human beings, in which chymase-containing mast cells predominate in connective tissues, including skin. The results suggest that more than one chymase subset is present, at least in duodenum. The possible occurrence of dual-specific chymase mast cells, as in other ruminants, is discussed. [less ▲]

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See detailDiagnostics of the jovian aurora deduced from ultraviolet spectroscopy: Model and HST/GHRS observations
Dols, V.; Gérard, Jean-Claude ULg; Clarke, J. T. et al

in Icarus: International Journal of Solar System Studies (2000), 147(1), 251-266

A model coupling an electron energy degradation code with a detailed synthetic spectrum of the H-2 Lyman and Werner band system is used to calculate the emerging auroral ultraviolet spectra from Jupiter's ... [more ▼]

A model coupling an electron energy degradation code with a detailed synthetic spectrum of the H-2 Lyman and Werner band system is used to calculate the emerging auroral ultraviolet spectra from Jupiter's atmosphere excited by electrons with different initial energy distributions. The atmospheric model is adapted from the vertical P-T profile measured by the Galileo probe and midlatitude model hydrocarbon photochemistry. Each altitude layer, with its own gas temperature, contributes to the emergent ultraviolet spectrum and the absorbers are vertically distributed within the source region of the auroral emissions. Examples of the calculated spectra are shown to validate the synthetic spectrum and to illustrate the importance of the electron energy distribution and the vertical structure. The model is then applied to the analysis of seven HST/GHRS spectra of the 1200-1700 Angstrom region obtained with 5-Angstrom resolution at various locations in the north and south Jovian aurora. These spectra have different color ratios which characterize the energy of the precipitated electrons, although they do not have a high enough spectral resolution to permit a determination of the H-2 temperature. We find that the characteristic energy of the assumed initial Maxwellian distribution ranges between 17 and 40 keV. A clear signature of acetylene absorption is observed near 1520, 1480, and 1440 Angstrom where the C2H2 cross section shows strong absorption peaks. The acetylene column abundance overlying the emission peak varies from 0.02 to 0.2 of the methane column. A better fit is obtained for some spectra when ethane absorption is added. The C2H6 column abundance varies from 0 to 0.5 of the methane column. These changes relative to methane are presumably the result of perturbations by heat released by the fast electron thermalization and/or perturbations to the hydrocarbon chemistry resulting from the production of H atoms by the aurora, A spectrum of the Io flux tube footprint and its trailing tail shows an ultraviolet color and hydrocarbon absorption quite similar to some of the main oval spectra, This observation suggests that the electrons of the Io flux tube are energized to a few tens of keV, similar to the electron precipitated in the main ovals and polar caps. Echelle spectra between 1216 and 1220 Angstrom at 0.07 Angstrom resolution are also compared with the model fitting best the mid-resolution spectra. It is found that the effective H-2 rovibrational temperature associated with the echelle spectra are significantly higher than predicted by the mid-latitude model. A large vertical temperature gradient just above the methane homopause due to large heating by auroral precipitation is a plausible explanation for this difference. (C) 2000 Academic Press. [less ▲]

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See detailTechnique for a rapid and efficient purification of the SHV-1 and PSE-2 beta-lactamases.
Bouillenne, Fabrice ULg; Matagne, André ULg; Joris, Bernard ULg et al

in Journal of Chromatography. B : Biomedical Sciences and Applications (2000), 737(1-2), 261-5

A simple procedure is described which results in an optimised resolution in molecular sieve chromatography. A sample exhibiting a large initial volume (about 20 ml) and conditioned in a buffer of low ... [more ▼]

A simple procedure is described which results in an optimised resolution in molecular sieve chromatography. A sample exhibiting a large initial volume (about 20 ml) and conditioned in a buffer of low ionic strength (<20 mM) by filtration through a 53-ml G25 molecular sieve column, is adsorbed on a 1.7-ml ion-exchange (SOURCE) column. The proteins are released by a 10-ml pulse of 1 M NaCl and the eluate directly injected onto a 120-ml Sephacryl S100-HR column. The very low volume of the eluate ensures optimal conditions and resolution for the molecular sieving process. The method is applied as the polishing step in the purification of the SHV-1 and PSE-2 beta-lactamases. It could easily be scaled up for the treatment of larger samples. [less ▲]

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See detailDes jeunes face aux institutions
Guillaume, Jean-François ULg

in Lien social et politiques (2000), 43(printemps), 113-120

Un constat semble s'impoesr à tout observateur des sociétés contemporaines : les jeunes générations, plus sensibles aux changements qui affectent les différentes sphères de la vie sociale ... [more ▼]

Un constat semble s'impoesr à tout observateur des sociétés contemporaines : les jeunes générations, plus sensibles aux changements qui affectent les différentes sphères de la vie sociale, expérimenteraient de nouveaux rapports aux institutions traditionnelles, tiraillés entre l'attachement et le détachement aux repères qui ont valu pour ceux qui les ont précédés. Mais on peut considérer que la jeunesse n'apparaît en tant qu'objet singulier qu'à travers une grille de lecture "institutionnalisée", largement portée par les intervenants sociaux et les acteurs politiques, et où s'affirme avant tout le dessein d'un encadrement, voire d'une réappropriation, des pratiques juvéniles. Il n'y aurait donc pas, dans les discours sur la jeunesse, de regard désintéressé, susceptible de considérer ses pratiques dans leur spécificité. Une approche sociologique de la jeunesse ne peut dès lors faire l'économie d'une réflexion critique sur les grilles de lecture communément admises et utilisées pour définir la condition contemporaine de la jeunesse. [less ▲]

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See detailPerturbed Proximal Point Algorithm with Nonquadratic Kernel
Brohé, Myrana; Tossings, Patricia ULg

in Serdica Mathematical Journal (2000), 26

Let H be a real Hilbert space and T be a maximal monotone operator on H. A well-known algorithm, developed by R.T. Rockafellar, for solving the problem "To find x in H such that 0 in Tx" is the proximal ... [more ▼]

Let H be a real Hilbert space and T be a maximal monotone operator on H. A well-known algorithm, developed by R.T. Rockafellar, for solving the problem "To find x in H such that 0 in Tx" is the proximal point algorithm. Several genaralizations have been considered by several authors: introduction of a perturbation, introduction of a variable metric in the perturbed algorithm, introduction of a pseudo-metric in place of the classical regularization, ... We summarize some of these extensions by taking into account a pseudo-metric as regularization and a perturbation in an inexact version of the algorithm. [less ▲]

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See detailLe paradoxe du passage a l'acte: l'agressivité n'est pas la violence
Dozot, Jean-Paul; Triffaux, Jean-Marc ULg; Wauthy, Jacques ULg et al

in Revue des Hôpitaux de Jour Psychiatriques et des Thérapies Institutionnelles (2000), 2

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See detailDe l'inapplicabilite de certaines recommandations therapeutiques en pratique: l'exemple du remboursement des medicaments hypolipidemiants en Belgique.
Scheen, André ULg

in Revue Médicale de Liège (2000), 55(4), 260-4

International guidelines from the Task Force of European and other Societies on Coronary Prevention and national guidelines from the Belgian Lipid Club recently emphasized the potential interest of ... [more ▼]

International guidelines from the Task Force of European and other Societies on Coronary Prevention and national guidelines from the Belgian Lipid Club recently emphasized the potential interest of adequate management of patients with even moderate hypercholesterolaemia. However, current criteria for the reimbursement of lipid-lowering drugs in Belgium do not allow to follow these guidelines. A solution should be urgently found in order to bridge the gap and permit practitioners, after diet failure, to effectively prescribe lipid-lowering agents in patients who should benefit in priority of such a treatment. It is certainly the case in secondary prevention as well as, in high risk individuals, in primary prevention, i.e. in situations where these drugs have provided enough evidence of their efficacy with an acceptable cost-benefit ratio. [less ▲]

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See detailLa maladie du hêtre : catastrophe écologique ou accident de la nature.
Rondeux, Jacques ULg

in Parcs & Réserves (2000), 55(3-4), 49

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See detailPolitique et usage des manuels scolaires en mathématiques et en sciences
Monseur, Christian ULg; Demeuse, Marc ULg

in Cahiers du Service de Pédagogie Expérimentale (SPE) (2000), (3-4), 177-183

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