References of "Scientific journals"
     in
Bookmark and Share    
Peer Reviewed
See detailInteresting Lines in the Infrared Solar Spectrum. II. Unblended Lines between 1.0 and 1.8µm
Ramsauer, J.; Solanki, S. K.; Biémont, Emile ULg

in Astronomy and Astrophysics. Supplement Series (1995), 113

Detailed reference viewed: 7 (3 ULg)
Full Text
Peer Reviewed
See detail'Overlapping Territories, Intertwined Histories': Cross-Culturality in Caryl Phillips's Crossing the River
Ledent, Bénédicte ULg

in Journal of Commonwealth Literature (1995), 30(1), 55-62

Detailed reference viewed: 490 (10 ULg)
Peer Reviewed
See detailThe Formation of National Elites de A. Kappeler (ed.)
Martiniello, Marco ULg

in Ethnic and Racial Studies (1995), 18(2), 421-422

Detailed reference viewed: 19 (0 ULg)
Peer Reviewed
See detailUse of shelter by grazing bulls: effect of climatic factors
Vandenheede, Marc ULg; Nicks, Baudouin ULg; Sheri, R. et al

in Animal Science (1995), 60

The time spent under a shelter by eight grazing fattening bulls of the Belgian Blue breed in each of 3 years consecutively for a total of 48 days was recorded using a time-lapse recorder with infrared ... [more ▼]

The time spent under a shelter by eight grazing fattening bulls of the Belgian Blue breed in each of 3 years consecutively for a total of 48 days was recorded using a time-lapse recorder with infrared illumination. During one grazing period, data were collected over 35 days (experiment 1). Observations were divided into 166 h with rain (20%) and 674 h without precipitation (80%). The average occupation rates were 15.4 (s.e. 29.1)% for the hours with rain and 4.5 (s.e. 14.8)% for the hours without precipitation (P < 0.001). The effect of intensity and duration of rain on the occupation rate was significant from 0.4 l/m(2) or from 2 h. Experiment 2 (13 days of observation) examined the influence of temperature and solar radiation on the use of shelter. The occupation rates were 21 (s.e. 23)% during the daylight hours (07.00 to 19.00 h), 6 (s.e. 8)% during the night (19.00 to 07.00 h) and 14 (s.e. 14)% during a 24-h period. The occupation rate between 07.00 and 19.00 h was significantly correlated to the mean daily temperature (r = 0.75), the maximum daily temperature (r = 0.86) and the direct solar radiation time (r = 0.60). When the maximum daily temperature exceeded 20 degrees C, the use of the shelter increased from 10 to 49% of the daylight hours. There was no significant difference between the live-weight gains of animals from the observed group and those of other bulls on an adjacent pasture without shelter. Nevertheless, these observations suggest that a shelter may improve the welfare of grazing cattle. [less ▲]

Detailed reference viewed: 40 (1 ULg)
Peer Reviewed
See detailBlues & African-American Literature. The Devil's Music in Novels of Nella Larsen,Gayl Jones and Toni Morrison. An Approach
Sacré, Robert ULg

in International Jazz Archives Journal (1995), vol.1(no.3), 93-106

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailArt (de l'affiche) et pouvoir
Duchesne, Jean-Patrick ULg

in Art&Fact (1995), 14

Detailed reference viewed: 9 (0 ULg)
See detailCabergoline in prolactinomen.
Abs, R.; Verhaert, G.; Demez, Pierre ULg et al

in Tijdschrift voor Geneeskunde (1995), 51

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailSimulating The Folding Of Small Proteins By Use Of The Local Minimum Energy And The Free Solvation Energy Yields Native-Like Structures
Brasseur, Robert ULg

in Journal of Molecular Graphics (1995), 13(5), 312-22

Assuming that the protein primary sequence contains all information required to fold a protein into its native tertiary structure, we propose a new computational approach to protein folding by ... [more ▼]

Assuming that the protein primary sequence contains all information required to fold a protein into its native tertiary structure, we propose a new computational approach to protein folding by distributing the total energy of the macromolecular system along the torsional axes. We further derive a new semiempirical equation to calculate the total energy of a macromolecular system including its free energy of solvation. The energy of solvation makes an important contribution to the stability of biological structures. The segregation of hydrophilic and hydrophobic domains is essential for the formation of micelles, lipid bilayers, and biological membranes, and it is also important for protein folding. The free energy of solvation consists of two components: one derived from interactions between the atoms of the protein, and the second resulting from interactions between the protein and the solvent. The latter component is expressed as a function of the fractional area of protein atoms accessible to the solvent. The protein-folding procedure described in this article consists of two successive steps: a theoretical transition from an ideal alpha helix to an ideal beta sheet is first imposed on the protein conformation, in order to calculate an initial secondary structure. The most stable secondary structure is built from a combination of the lowest energy structures calculated for each amino acid during this transition. An angular molecular dynamics step is then applied to this secondary structure. In this computational step, the total energy of the system consisting of the sum of the torsional energy, the van der Waals energy, the electrostatic energy, and the solvation energy is minimized. This process yields 3-D structures of minimal total energy that are considered to be the most probable native-like structures for the protein. This method therefore requires no prior hypothesis about either the secondary or the tertiary structure of the protein and restricts the input of data to its sequence. The validity of the results is tested by comparing the crystalline and computed structures of four proteins, i.e., the avian and bovine pancreatic polypeptide (36 residues each), uteroglobin (70 residues), and the calcium-binding protein (75 residues); the C alpha-C alpha maps show significant homologies and the position of secondary structure domains; that of the alpha helices is particularly close. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailAssessment of lipoxygenase activity in seed extracts from 35 plant species
Fauconnier, Marie-Laure ULg; Vanzeveren, E.; Marlier, Michel et al

in Grasas y Aceites (1995), 46(1), 6-10

Lipoxyganase activity in 35 seed extracts was determined an the basis of hydroperoxide synthesis using linoleic acid as substrate. The results referring to the extracted protein content show that several ... [more ▼]

Lipoxyganase activity in 35 seed extracts was determined an the basis of hydroperoxide synthesis using linoleic acid as substrate. The results referring to the extracted protein content show that several species of Vigna (V. unguiculata, V. radiata and V. mungo) and one of Trifolium exhibit stronger lipoxygenase activity than soybean enzyme extracts. The pH-activity relationship was also established for 4 very active samples. GCMS analysis revealed equal amounts of 9- and 13 hydroperoxides of linoleic acid In these seed extracts, indicating no enzyme positional specificity. [less ▲]

Detailed reference viewed: 36 (11 ULg)
See detailSixièmes Journées scientifiques de Télédétection : télédétection des espaces urbains et péri-urbains
Donnay, Jean-Paul ULg

in Lettre d'information du Réseau de Télédétection de l'UREF (1995), 28

Detailed reference viewed: 12 (1 ULg)
Peer Reviewed
See detailEffects of a new oral contraceptive containing an antimineralocorticoid progestogen, drospirenone, on the renin-aldosterone system, body weight, blood pressure, glucose tolerance, and lipid metabolism.
Oelkers, W.; Foidart, Jean-Michel ULg; Dombrovicz, N. et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(6), 1816-21

Combined hormonal oral contraceptives (OCs) may lead to a mild rise in blood pressure and body weight. In rare instances, large increments in blood pressure are measured. We investigated the effect of a ... [more ▼]

Combined hormonal oral contraceptives (OCs) may lead to a mild rise in blood pressure and body weight. In rare instances, large increments in blood pressure are measured. We investigated the effect of a combination of ethinyl estradiol (EE) plus a progestogen with antimineralocorticoid, i.e. natriuretic, properties [Drospirenone (DRSP)] on body weight, blood pressure, the renin-aldosterone system, atrial natriuretic factor, plasma lipids, and glucose tolerance. It is anticipated that this will lead to the development of an OC that does not raise body weight or blood pressure. Four groups of 20 women each received 30 micrograms EE plus 3 mg DRSP (group A), 20 micrograms EE plus 3 mg DRSP (group B), 15 micrograms EE plus 3 mg DRSP (group C), and, as a control OC, 30 micrograms EE plus 150 micrograms levonorgestrel (Microgynon, Schering; group D) for 6 months. During the OC-free control cycles before and after treatment and throughout treatment, the target parameters were measured. Between the pretreatment cycle and the sixth treatment cycle, mean body weight fell by 0.8 to 1.7 kg in groups A, B, and C (P < 0.05 vs. D), whereas it rose by 0.7 kg in group D. Systolic and diastolic blood pressures fell by 1-4 mm Hg in groups A, B, and C (significant for A and C vs. D) and increased by 1-2 mm Hg in group D. Renin substrate rose equally in all groups (P < 0.05), whereas PRA and plasma aldosterone rose significantly only in the DRSP groups, presumably due to sodium loss. In the DRSP groups, high density lipoprotein cholesterol rose (P < 0.05), in contrast to group D. Low density lipoprotein cholesterol fell slightly (P > 0.05), whereas triglyceride levels showed a stronger increase in the DRSP groups (P < 0.05) than in group D. All groups attained good cycle control; group A had the best. Side-effects were minimal. To our knowledge, this is the first report on a combined OC that leads to a small decrease in body weight and blood pressure. It may be especially beneficial for women susceptible for a gain in weight and a rise in blood pressure. The potential of a new oral contraceptive (OC) containing drospirenone (DRSP) to avert the moderate increases in body weight and blood pressure often associated with use of existing combined OCs was investigated in a study of four groups of 20 German women each. Group A received 30 mcg of ethinyl estradiol (EE) and 3 mg of DRSP, Group B was administered 20 mcg of EE and 3 mg of DRSP, Group C received 15 mcg of EE and 3 mg of DRSP, and Group D was given a standard OC containing 30 mcg of EE and 150 mcg of levonorgestrel. Between the pretreatment cycle and the last (sixth) treatment cycle, mean body weight fell by 0.8-1.7 kg in Groups A, B, and C, but rose by 0.7 kg in Group D. Systolic and diastolic blood pressures fell by 1-4 mm Hg in Groups A, B, and C and rose by 1-2 mm Hg in Group D. Renin substrate rose equally in all four groups, while plasma renin activity, plasma aldosterone, and high density lipoprotein cholesterol rose significantly only in the three DRSP groups and serum triglyceride levels were significantly higher in Group D than in the three DRSP groups. Glucose tolerance increases were similar in all four groups. Finally, all groups--but especially Group A--experienced good cycle control and there were no serious side effects. These findings suggest that a combined OC containing DRSP may be especially beneficial for women who have a tendency to gain weight or experience a rise in blood pressure while taking OCs. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailIncreased frequency of bronchial hyperresponsiveness in patients with inflammatory bowel disease.
Louis, Edouard ULg; Louis, Renaud ULg; Drion, V. et al

in Allergy (1995), 50(9), 729-33

Although bronchopulmonary manifestations are rare in inflammatory bowel disease (IBD), subclinical abnormalities have been described in up to 50% of cases. The pathophysiology of these abnormalities ... [more ▼]

Although bronchopulmonary manifestations are rare in inflammatory bowel disease (IBD), subclinical abnormalities have been described in up to 50% of cases. The pathophysiology of these abnormalities remains unknown. However, a latent inflammation of the bronchial mucosa secondary to the inflammation of the intestinal mucosa has been suggested. This subclinical inflammation may lead to increased bronchial responsiveness. We studied the bronchial responsiveness in 38 inflammatory bowel disease (IBD) patients, using the methacholine test. Bronchial hyperresponsiveness was defined by a PC20M < 16 mg/ml. Twenty-four healthy controls were also studied. There was no significant difference in baseline FEV1 between IBD patients and controls. However, there was a significantly greater fall in FEV1 in the IBD patients at the concentrations of methacholine tested. The frequency of bronchial hyperresponsiveness was significantly higher in the IBD population (45%) than in controls (17%; P < 0.03). Atopy, defined by skin test, was more common in IBD patients (42%) than in controls (21%). Even when only nonatopic subjects were considered, the frequency of bronchial hyperresponsiveness was significantly higher in IBD patients (41%) than in controls (5%; P < 0.02). Thus, subclinical bronchial hyperresponsiveness is common in IBD, and may be considered a further extraintestinal manifestation. [less ▲]

Detailed reference viewed: 32 (3 ULg)
Peer Reviewed
See detailPostweaning housing conditions and partner preference and sexual behavior of neonatally ATD-treated male rats.
Bakker, Julie ULg; van Ophemert, J.; Slob, A. K.

in Psychoneuroendocrinology (1995), 20(3), 299-310

Male rats were neonatally treated with cholesterol or a substance that blocks the aromatization of testosterone to estradiol (1,4,6-androstatriene-3,17-dione: ATD). At weaning (21 days) they were either ... [more ▼]

Male rats were neonatally treated with cholesterol or a substance that blocks the aromatization of testosterone to estradiol (1,4,6-androstatriene-3,17-dione: ATD). At weaning (21 days) they were either housed alone or in small groups (2-3 animals) and tested for partner preference behavior (PPB) in adulthood. Choice was between an estrous female and an active male (Part I) and between an estrous female and an ATD-male (Part II). Tests were carried out in a 3-compartment box. Social isolation did not have major effects on PPB except when sexual interaction with the stimulus animals was prevented (Part I). In this case, isolates (ATD and control) showed higher preference scores (PS) for the estrous female and spent less time in the empty middle compartment. When the choice was between an estrous female and an ATD-male, partner PS decreased in all males, most clearly in ATD-males. The latter animals spent more time with the stimulus ATD-male than they had done in previous PPB tests with the normal stimulus male. In contrast to partner preference behaviors, sexual behavior was clearly affected by social isolation. Isolates (ATD and control) displayed lower frequencies of mounts and intromissions. These effects persisted over consecutive tests. Ejaculation was not affected. In conclusion, the present results confirm earlier findings about the significance of neonatal endocrine conditions for the organization of adult PPB in male rats. The presence or absence of social conspecifics after weaning appears to have little influence on adult PPB. [less ▲]

Detailed reference viewed: 2 (0 ULg)
Peer Reviewed
See detailComposition n'est pas raison? Une contrefaçon liégeoise de la Théorie de l'impôt de Mirabeau (1761)
Droixhe, Daniel ULg

in De Gulden Passer = Le compas d'or (1995), 73

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailContribution de la chimie macromoléculaire à l'élaboration de matériaux colloïdaux et microparticulaires destinés à la vectorisation de médicaments
Grandfils, Christian ULg; Flandroy, P.; Jérôme, Robert ULg

in Innovation et Technologies en Biologie et en Médecine (1995), 16

Various colloidal and microparticular vector have been tailored during the last few years in order to promote the transport , the protection and the orientation of the drug towards its pharmacological ... [more ▼]

Various colloidal and microparticular vector have been tailored during the last few years in order to promote the transport , the protection and the orientation of the drug towards its pharmacological target. The aim of the paper is to give some insight about the contribution of the macromolecular engineering for the development of two vectors : microparticles of a control biodegradation rate designed for the chemoembolization of hepatocarcinoma , amphiphile copolymeric micelles able to transport drug within the targeted cells. Starting from a synthetic polymer : polylactide, degradation rate caracteristics , and functionalities can be tailored for the intended applications. One of the major problems , and largely underestimated in the past , for the successfull design of polymeric carrier is to take into account the interaction of proteins with polymer surfaces. [less ▲]

Detailed reference viewed: 45 (4 ULg)
Peer Reviewed
See detailExploration biologique actuelle du métabolisme du fer
Charlier, Corinne ULg; Plomteux, Guy ULg

in Revue de la Médecine Générale (1995), 123

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailLa thermographie infrarouge dans l'étude de la thermorégulation : revue de la littérature
Spruyt, P.; Ghafir, Y.; Art, Tatiana ULg et al

in Annales de Médecine Vétérinaire (1995), 139

Infrared thermography is a method to assess the skin temperature by means of a camera equipped with an infrared detector. It makes thermograms that represents the surface temperature of skin patterns ... [more ▼]

Infrared thermography is a method to assess the skin temperature by means of a camera equipped with an infrared detector. It makes thermograms that represents the surface temperature of skin patterns. This article summerizes the different information about thermography and thermoregulation of horses. In fact, lots of information exist about thermoregulation of most of the domestic animais but very little exists as far as horses are concerned. Two different types of devices are used for thermography : contacting and noncontacting which measure the infrared radiation either with a thermometer or with a themographic camera. The blood circulation dictates the thermic model which is at the origin of thermographic interpretation. This latter is a good method helping to study thermoregulation. [less ▲]

Detailed reference viewed: 346 (13 ULg)
Peer Reviewed
See detailA general functional characterization of the microlocal singularities
Bastin, Françoise ULg; Laubin, Pascal

in Journal of Mathematical Sciences, The University of Tokyo (1995), 2(1), 155-164

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailDeux chansons relatives à des imprimeries liégeoises du XVIIIe siècle
Droixhe, Daniel ULg

in Bulletin de la Société Royale Le Vieux-Liège (1995), 268

Detailed reference viewed: 41 (5 ULg)
Peer Reviewed
See detailPostgastroplasty recovery of ideal body weight normalizes glucose and insulin metabolism in obese women.
Letiexhe, Michel ULg; Scheen, André ULg; Gerard, Pascale ULg et al

in Journal of Clinical Endocrinology and Metabolism (1995), 80(2), 364-9

To study the metabolic effects of normalizing body weight, a frequently sampled iv glucose tolerance test (0.3 g/kg) was performed before [body mass index (BMI), 37.7 +/- 0.5 kg/m2] and 14 +/- 2 months ... [more ▼]

To study the metabolic effects of normalizing body weight, a frequently sampled iv glucose tolerance test (0.3 g/kg) was performed before [body mass index (BMI), 37.7 +/- 0.5 kg/m2] and 14 +/- 2 months after successful gastroplasty (BMI, 23.7 +/- 0.6 kg/m2) in eight obese women and, for comparison, in eight age- and weight-matched nonobese control women (BMI, 23.6 +/- 0.7 kg/m2). All subjects had normal oral glucose tolerance. The insulin secretion rate (ISR) was derived by deconvolution of plasma C-peptide levels and the insulin MCR (MCRI) by dividing the 0-180 min area under the curve (AUC) of ISR by that of plasma insulin levels (IRI). The insulin sensitivity index (SI) and the glucose effectiveness index (SG) were calculated using Bergman's minimal model. Before gastroplasty, obese subjects showed higher AUC-IRI (P < 0.001) and AUC-ISR (P < 0.02), lower MCRI (P < 0.005) and SI (P < 0.002), but similar SG values, compared to nonobese controls. After gastroplasty, the AUC-IRI dramatically decreased, due to both a reduction of AUC-ISR (from 58,252 +/- 8,437 to 36,675 +/- 4,274 pmol; P < 0.05) and an increase in MCRI (from 658 +/- 117 to 1,299 +/- 127 mL/min.m-2; P < 0.02). SI significantly rose from 4.74 +/- 0.74 to 9.15 +/- 0.96 10(-5) min-1/pmol.L (P < 0.01), whereas SG remained unchanged. All of these parameters became similar to those in nonobese controls (respectively, 32,522 +/- 3,458, 1,180 +/- 101, and 8.48 +/- 1.25; all P = NS). In conclusion, after gastroplasty-induced normalization of body weight, postobese women recover normal insulin secretion, clearance, and action on glucose metabolism. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailBronchial hyperresponsiveness in active coke-oven workers.
Corhay, Jean-Louis ULg; Bury, Thierry ULg; Louis, Renaud ULg et al

in Archives of Public Health (1995), 53

Detailed reference viewed: 15 (3 ULg)
Full Text
Peer Reviewed
See detailOn the presence of a day-of-the-week effect in the foreign exchange market
Corhay, Albert ULg; Fatemi, Ali M; Tourani-Rad, Alireza

in Managerial Finance (1995), 21(8), 32-43

The purpose of this paper is to study the presence of the day-of-the-week effect in the foreign exchange market using an extensive data set of six exchange rates and employing two alternative types of ... [more ▼]

The purpose of this paper is to study the presence of the day-of-the-week effect in the foreign exchange market using an extensive data set of six exchange rates and employing two alternative types of distributional assumptions and also distribution-free tests. Our findings support the hypotheses of higher Wednesday returns prior to October 1, 1981, for the British pound, the Canadian dollar, the Deutsch mark, the French franc, the Swiss franc and the Japanese yen. Pursuant to the change in the settlement procedures which took effect October 1, 1981, the differential Wednesday returns disappear. [less ▲]

Detailed reference viewed: 44 (1 ULg)
Peer Reviewed
See detailAnomalous pion decay in effective QCD at finite temperature
Blaschke, David; Jaminon, Martine ULg; Kalinovsky, Yu. L. et al

in Nuclear Physics A (1995), 592

The width of the process π0 → γγ is calculated within effective QCD at finite temperature. We use two different effective models which describe chiral symmetry breaking and its restoration at finite ... [more ▼]

The width of the process π0 → γγ is calculated within effective QCD at finite temperature. We use two different effective models which describe chiral symmetry breaking and its restoration at finite temperature in SU(2)ƒ: (i) an effective lagrangian with a nonlocal separable interaction kernel, (ii) A- and B-scaledNJL lagrangians which implement the scale anomaly of QCD. We calculate the temperature dependences of the quark and pion masses as well as of the pion-park-antiquark coupling strength below and above the double quark mass threshold. At zero temperature we obtain a fairly good agreement with the experimental value for the decay width. The temperature dependence of the decay width is related to that of the pion mass. Γπ0 → γγ is enhanced in the vicinity of the Mott temperature (Mπ = 2mq) for the nonlocal as well as for the A-scaled model and suppressed for the B-scaled model. We compare our results with the SU(2) Nambu-Jona-Lasinio model which is contained as a limiting case in our approach. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailPhysical Properties of Particles of Ipratropium and Clenbuterol Generated by Equipment Suitable for the Inhalation of Drugs by Calves
Genicot, Bruno; Lapp, K.; Close, Roland et al

in Research in Veterinary Science (1995), 59(2), 164-167

When solutions of ipratropium and clenbuterol were atomised at 300 kPa and 450 kPa in equipment suitable for the inhalation of drugs by calves, the numbers, velocities and diameters of the particles ... [more ▼]

When solutions of ipratropium and clenbuterol were atomised at 300 kPa and 450 kPa in equipment suitable for the inhalation of drugs by calves, the numbers, velocities and diameters of the particles produced were similar. When the pressure was increased to 600 kPa more of the particles were less than 2 microns in diameter and fewer were more than 7 microns in diameter, the fractions of the total mass of the solution generated in these size ranges were similarly increased and decreased, and the velocities of the particles were increased. At any given pressure, the numbers of particles of different sizes, and the proportions of the total mass generated, were similar for the solutions of ipratropium and clenbuterol, but a solution of saline produced more particles with a diameter less than 3 microns. Particles from the solution of ipratropium had the highest velocity and particles from the solution of clenbuterol had the lowest velocity. [less ▲]

Detailed reference viewed: 50 (0 ULg)
Full Text
Peer Reviewed
See detailThe Yxxl Signaling Motifs Of The Bovine Leukemia-Virus Transmembrane Protein Are Required For In-Vivo Infection And Maintenance Of High Viral Loads
Willems, Luc ULg; Gatot, Js.; Mammerickx, M. et al

in Journal of Virology (1995), 69(7),

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailEffects of PEEP on systemic vascular compliance in intact pigs
LAMBERMONT, Bernard ULg; DETRY, Olivier ULg; FOSSION, A et al

in Pflügers Archiv : European Journal of Physiology (1995), 430

Detailed reference viewed: 11 (0 ULg)
See detailLa naissance de l'écriture au Proche-Orient
Tunca, Önhan ULg

in Le génie de l'Homme. Des origines à l'écriture (1995)

Detailed reference viewed: 19 (0 ULg)
Peer Reviewed
See detailDifferential distribution of single-stranded DNA, double-stranded DNA, and RNA in adenovirus-induced intranuclear regions of HeLa cells.
Thiry, Marc ULg; Puvion-Dutilleul, F.

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1995), 43(8), 749-59

We investigated in great detail the fine spatial distribution of nucleic acids within adenovirus-infected HeLa cells by various immunogold labeling procedures. To detect DNA, we used the in situ terminal ... [more ▼]

We investigated in great detail the fine spatial distribution of nucleic acids within adenovirus-infected HeLa cells by various immunogold labeling procedures. To detect DNA, we used the in situ terminal deoxynucleotidyl transferase-immunogold technique. In addition to the expected evident label over the condensed host chromatin and the structures containing viral double- and single-stranded DNA, label was consistently revealed over round fibrillar spots. By contrast, other virus-induced substructures, such as compact rings, crystalloids, clear amorphous inclusions, and electron-dense amorphous inclusions, displayed no significant label. Except for the viral single-stranded DNA accumulation sites, identical labeling pattern was obtained with the in situ nick-translation-immunogold method. We further labeled the sections with anti-RNA antibodies. Label was present not only over the cytoplasm and the intranuclear fibrillogranular network but also quite obviously over the compact rings and interchromatin granule clusters. None was seen over the other nuclear structures of infected cells, notably over the fibrillar spots. We suggest that these fibrillar spots might be involved in the formation of the viral, non-encapsidated, double-stranded DNA storage site. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailViolence et justice
Steinmetz, Rudy ULg

in Avancées (1995)

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailInvolvement of MMP2 and MMP9 in the development of abdominal aortic aneurysms.
SakalihasanN, Natzi ULg; DELVENNE, Philippe ULg; Nusgens, Betty ULg et al

in Cell Biology International (1995), 19(3), 250-51

Detailed reference viewed: 10 (7 ULg)
Peer Reviewed
See detailVenous Allografts for Critical Limb Ischaemia
VAN DAMME, Hendrik ULg; CREEMERS, Etienne ULg; Limet, Raymond ULg

in Acta Chirurgica Belgica (1995), 95(1, Jan-Feb), 14-20

In as many as 25% of the patients with critical lower limb ischaemia, a suitable autogenous vein graft is not available. Patency rate of prosthetic grafts is disappointing at the infrageniculate level ... [more ▼]

In as many as 25% of the patients with critical lower limb ischaemia, a suitable autogenous vein graft is not available. Patency rate of prosthetic grafts is disappointing at the infrageniculate level. Recently, there is renewed interest in the use of preserved venous allografts as an alternate conduit in patients with threatened ischaemic limbs. From 1991 to 1993, the authors revascularized 12 ischaemic limbs with venous allografts (10 preserved at 4 degrees C, 2 Varivas grafts). The indication for operation was rest pain in five patients and tissue necrosis in seven. Nine patients had one or more previous ipsilateral revascularizations. The at 4 degrees C preserved venous allografts were conserved for a near period of 2 months. In only half (5/10) of the cases ABO isocompatibility could be respected. Distal anastomosis was always below the knee. In 6 patients, the anastomosis was at tibial level. There was one rupture of a Varivas graft on the fifth postoperative day, necessitating ligation of the graft and subsequent amputation. All other grafts remained patent for a mean period of 10.6 months. Actually, 6 grafts are still open. Most patients were given aspirin. Only three patients were under oral coumarin anticoagulation in an effort to prevent early graft failure. The limb salvage rate is 67% (8/12) (mean follow-up of 13 months). The authors discuss the clinical experience with venous allografts as published in recent literature. Use of venous allografts for arterial reconstruction in humans was met with limited success so far. The one year patency approximates 50%. Venous allografts progress in an ineluctable way to a fibrous conduit.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 6 (1 ULg)
Peer Reviewed
See detailFra Stein til Waldrop: sprogdigtningen og de nye prosadigtere
Delville, Michel ULg; Schatz-Jakobsen, Claus

in Den Blå Port: Tidsskrift for Litteratur (1995)

Detailed reference viewed: 9 (2 ULg)
Peer Reviewed
See detailApplication des transects pluviométriques spatio-temporels annuels à l’ensemble du territoire sahélien.
Ozer, Pierre ULg

in Publications de l'Association Internationale de Climatologie (1995), 8

Detailed reference viewed: 15 (1 ULg)
Full Text
See detailBovine respiratory disease complex : an european perspective
Lekeux, Pierre ULg

in Bovine Practitioner (1995), 29

Respiratory disease is the principal cause of loss of young cattle worldwide. The syndrome arises from a number of factors, including those involving the animal, e.g., age, general condition and immune ... [more ▼]

Respiratory disease is the principal cause of loss of young cattle worldwide. The syndrome arises from a number of factors, including those involving the animal, e.g., age, general condition and immune status; its environment, e.g., changes in food, temperature and humidity that lead to stress; and the presence of infectious agents, e.g., bacteria, viruses and mycoplasmas. The syndrome, in a method preferred by the author, can be classified into four grades; Grade 1, subclinical disease; Grade 2, compensated clinical disease (at this stage, the inflammatory reaction generated tends to limit the impact of the disease on the animal); Grade 3, noncompensated clinical disease (at this stage, the inflammatory reaction is excessive and must be controlled); and Grade 4, irreversible clinical disease (which threatens the animal's survival). The increase in frequency and economy impact of bovine respiratory disease complex can be correlated with the escalating industrialization of cattle production. In intensive operations, commingling of animals from multiple sources, exposure to many organisms, stress and management practices are all factors that can lead to disease. The predisposition of cattle, especially beef calves, to respiratory problems is related to their lack of functional pulmonary hardiness. Selection of breeds that demonstrate adequate pulmonary function and sufficient ventilatory reserve may help in the control of the bovine respiratory disease complex, but this approach is difficult to implement and slow to produce results. Prophylactic measures, including vaccination programs and modifying management practices to reduce stress, also have a place in preventing the bovine respiratory disease complex. Unfortunately, these measures are not always easy to put into operation and cannot completely eradicate the problem. Therapeutic strategies to minimize the economy impact of the syndrome include use of appropriate antibacterial therapy, modulation of the pulmonary inflammatory reaction and correction of mechanical disorders. [less ▲]

Detailed reference viewed: 173 (6 ULg)
Full Text
Peer Reviewed
See detailInverse Modulation of Intraepithelial Langerhans' Cells and Stromal Macrophage/Dendrocyte Populations in Human Papillomavirus-Associated Squamous Intraepithelial Lesions of the Cervix
Al-Saleh, Walid; Delvenne, Philippe ULg; Arrese Estrada, Jorge ULg et al

in Virchows Archiv : An International Journal of Pathology (1995), 427(1), 41-8

Ninety-four cervical biopsies from normal tissue to high-grade squamous intraepithelial lesion (SILs) were examined for the presence of intraepithelial Langerhans' cells and subpopulations of stromal ... [more ▼]

Ninety-four cervical biopsies from normal tissue to high-grade squamous intraepithelial lesion (SILs) were examined for the presence of intraepithelial Langerhans' cells and subpopulations of stromal macrophages/dendrocytes by immunohistochemistry using anti-S100, -L1, -CD68 and -factor XIIIa antibodies. Human papillomavirus (HPV) detection was performed in all cases by using first a mixture of DNA probes for 14 HPV types commonly found in anogenital biopsies at low stringency conditions (Tm -40 degrees C) and by reanalyzing the tissues at high stringency (Tm -10 degrees C) with HPV 6/11, 16/18 and 31/33/35 biotinylated probe cocktails and individual digoxigenin-labelled probes. SILs and metaplastic tissues were significantly associated with a depletion of S100-positive intraepithelial Langerhans' cells when compared with normal epithelium. In contrast, there was a significant increase in L1-positive stromal macrophages in SIL biopsies compared with normal or metaplastic cervix. A significantly higher density of CD68-positive macrophages was also observed in high-grade SILs compared with normal or metaplastic biopsies and with low-grade SILs. The density of factor XIIIa-positive dendrocytes was found to be higher in SILs compared with metaplastic tissues and in high-grade SILs when compared with normal cervical biopsies. No specific relationship was found between the densities of these cells and the HPV type detected in SILs separated into low grade and high grade. The significance of this inverse modulation of intraepithelial Langerhans' cells and stromal macrophages/dendrocytes in normal and SIL biopsies is discussed in relation to HPV infection and malignant transformation. [less ▲]

Detailed reference viewed: 25 (4 ULg)
Full Text
Peer Reviewed
See detail3-Benzamido-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide
Dupont, L.; De Tullio, Pascal ULg; Pirotte, Bernard ULg et al

in Acta Crystallographica (1995), C51

Detailed reference viewed: 9 (2 ULg)
Full Text
See detailVaricella-zoster virus : Introduction
Rentier, Bernard ULg

in Neurology (1995), 45(12, suppl. 8), 8

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detail3-Methyl-4H-pyrido[2,3-e]-1,2,4-thiadiazine 1,1-dioxide
Dupont, L.; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Acta Crystallographica (1995), C51

Detailed reference viewed: 3 (2 ULg)
Full Text
Peer Reviewed
See detailMediators in critical illness
Deby, Ginette ULg; Lamy, Maurice ULg

in Current Anaesthesia and Critical Care (1995), 6(1), 3-9

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailNouveaux activateurs des canaux potassiques: conception, pharmacologie et perspectives thérapeutiques
Pirotte, Bernard ULg; Fontaine, J.; Lebrun, P.

in Journal de Pharmacie de Belgique (1995), 50

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailRecent advances in ring-opening polymerization of lactones and related compounds
Lögfren, Anders; Albertsson, Ann-Christine; Dubois, Philippe ULg et al

in Journal of Macromolecular Science - Reviews in Macromolecular Chemistry and Physics (1995), C35(3), 379-418

Polyesters are currently synthesized by a step-growth mechanism from a mixture of a diol and a diacid (or a diacid derivative), or from a hydroxy-acid when available. Ring-opening polymerization (ROP) of ... [more ▼]

Polyesters are currently synthesized by a step-growth mechanism from a mixture of a diol and a diacid (or a diacid derivative), or from a hydroxy-acid when available. Ring-opening polymerization (ROP) of lactones and related compounds is an alternative method for the synthesis of aliphatic polyesters. Comparison of these two mechanisms is clearly in favor of the polyaddition process. The drawbacks of polycondensation are well known:high temperatures and long reaction times are required to produce high-molecular-weight chains. Even though conversion of the hydroxyl and acid group is close to completion, any departure from the reaction stoichiometry has a very detrimental effect on the chain length. [less ▲]

Detailed reference viewed: 134 (6 ULg)
Full Text
Peer Reviewed
See detailÉvaluation de l’impact de la végétation sur le rayonnement énergétique (aide à la conception architecturale et urbanistique)
Teller, Jacques ULg

in Publications de l'Association Internationale de Climatologie (1995), 8

Detailed reference viewed: 53 (19 ULg)
Full Text
Peer Reviewed
See detailHypnosis as adjunct therapy in conscious sedation for plastic surgery
Faymonville, Marie ULg; Fissette, Jean ULg; Mambourg, P.-H. et al

in Regional Anesthesia (1995), 20(2), 145-151

Detailed reference viewed: 84 (4 ULg)
Peer Reviewed
See detailOn the charge transfer pathway in the Merocyanine 540 triplet state quenching by nitroxyl radical
Hoebeke, Maryse ULg; Van de Vorst, A.

in Photochemistry & Photobiology (1995), 61

Detailed reference viewed: 1 (1 ULg)
Peer Reviewed
See detailKozani-Grevena Earthquake and the Active tectonics of Northern Greece
Meyer, B.; Armijo, R.; Hubert, Aurelia ULg et al

Scientific journal (1995)

Detailed reference viewed: 4 (1 ULg)
Peer Reviewed
See detailGrecs et Latins face aux langues étrangères. Contribution à l'étude de la diversité linguistique dans l'antiquité classique
Rochette, Bruno ULg

in Revue Belge de Philologie et d'Histoire (1995), 73(1), 5-16

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailNucleic acid compartmentalization within the cell nucleus by in situ transferase-immunogold techniques.
Thiry, Marc ULg

in Microscopy Research and Technique (1995), 31(1), 4-21

In the present review, we report on recent results obtained by in situ transferase-immunogold techniques as to the ultrastructural distribution of DNA and RNA within the cell nucleus. Special emphasis is ... [more ▼]

In the present review, we report on recent results obtained by in situ transferase-immunogold techniques as to the ultrastructural distribution of DNA and RNA within the cell nucleus. Special emphasis is placed on the various nucleolar components and the various enigmatic structures of the extranucleolar region: interchromatin granules, coiled bodies, and simple nuclear bodies. These data are discussed in the light of our current understanding of the functional organization of the cell nucleus. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailFunctional analysis of the Antirrhinum floral homeotic DEFICIENS gene in vivo and in vitro by using a temperature-sensitive mutant.
Zachgo, S.; Silva, E de A; Motte, Patrick ULg et al

in Development (1995), 121(9), 2861-75

Flowers of the temperature-sensitive DEFICIENS (DEF) mutant, def-101, display sepaloid petals and carpelloid stamens when grown at 26 degrees C, the non-permissive temperature. In contrast, when ... [more ▼]

Flowers of the temperature-sensitive DEFICIENS (DEF) mutant, def-101, display sepaloid petals and carpelloid stamens when grown at 26 degrees C, the non-permissive temperature. In contrast, when cultivated under permissive conditions at 15 degrees C, the morphology of def-101 flowers resembles that of the wild type. Temperature shift experiments during early and late phases of flower development revealed that second and third whorl organ development is differentially sensitive to changes in DEF expression. In addition, early DEF expression seems to control the spatially correct initiation of fourth whorl organ development. Reduction of the def-101 gene dosage differentially affects organogenesis in adjacent whorls: at the lower temperature development of petals in the second whorl and initiation of carpels in the centre of the flower is not affected while third whorl organogenesis follows the mutant (carpelloid) pattern. The possible contribution of accessory factors to organ-specific DEF functions is discussed. In situ analyses of mRNA and protein expression patterns during def-101 flower development at 15 degrees C and at 26 degrees C support previously proposed combinatorial regulatory interactions between the MADS-box proteins DEF and GLOBOSA (GLO), and provide evidence that the autoregulatory control of DEF and GLO expression by the DEF/GLO heterodimer starts after initiation of all organ primordia. Immunolocalisation revealed that both proteins are located in the nucleus. Interestingly, higher growth temperature affects the stability of both the DEF-101 and GLO proteins in vivo. In vitro DNA binding studies suggest that the temperature sensitivity of the def-101 mutant is due to an altered heterodimerisation/DNA-binding capability of the DEF-101 protein, conditioned by the deletion of one amino acid within the K-box, a protein region thought to be involved in protein-protein interaction. In addition, we introduce a mutant allele of GLO, glo-confusa, where insertion of one amino acid impairs the hydrophobic carboxy-terminal region of the MADS-box, but which confers no strong phenotypic changes to the flower. The strong mutant phenotype of flowers of def-101/glo-conf double mutants when grown in the cold represents genetic evidence for heterodimerisation between DEF and GLO in vivo. The potential to dissect structural and functional domains of MADS-box transcription factors is discussed. [less ▲]

Detailed reference viewed: 12 (2 ULg)
See detailTémoignages de la spiritualité néolithique au Proche-Orient
Tunca, Önhan ULg

in Le génie de l'Homme. Des origines à l'écriture (1995)

Detailed reference viewed: 27 (1 ULg)
Full Text
Peer Reviewed
See detailMise au point d’une classification automatique des cartes synoptiques d’altitude et application à l’étude des incendies de forêts
Mabille, Georges; Geron, Jean-Marc; Funcken, Philippe et al

in Publication Association Internationale de Climatologie (1995), 8

Detailed reference viewed: 41 (15 ULg)
Full Text
Peer Reviewed
See detailCarte des sols de Belgique et SIG : un traitement préalable visant à la concordance géométrique
Lejeune, Philippe ULg

in Bulletin des Recherches Agronomiques de Gembloux (1995), 30(4), 339-351

Almost half (40 %) of the sheets of the Belgian Soil Map (scale 1/20,000) has been produced on a topographical background showing geometrical differences, sometimes important, with the basic topographic ... [more ▼]

Almost half (40 %) of the sheets of the Belgian Soil Map (scale 1/20,000) has been produced on a topographical background showing geometrical differences, sometimes important, with the basic topographic background produced at present by the National Institute of Geography. Twenty percent of control points georeferenced on a test sheet show differences higher than 80 meters between the two reference systems. A simple method is proposed to match up inaccurate sheets of the soil map with the basic topographic background and to reduce these differences. It is based on control points evenly distributed on the sheet to be corrected and georeferenced in the two systems. A translation is applied to the poiints digitised on the soil map, corresponding to a weighed mean of the differences observed on the nearest control points, the weighing factor being computed on the basis of distances between control points and the element to be corrected. This procedure is integrated in a program running on a PC in Arc/Info environment. The entire correction process (identification of control points, preparation of files, program execution) takes less than three hours per sheet. [less ▲]

Detailed reference viewed: 138 (12 ULg)
Full Text
Peer Reviewed
See detailSpecific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.
Weynants, V.; Godfroid, J.; Limbourg, B. et al

in Journal of Clinical Microbiology (1995), 33(3), 706-12

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of ... [more ▼]

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of cytoplasmic proteins from Brucella melitensis B115 was used as a specific antigenic stimulus in bovine whole blood culture. Supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (IFN-gamma) content by using a commercial sandwich enzyme-linked immunosorbent assay kit. The IFN-gamma assay was evaluated with 10 heifers during the course (80 days) of an experimental infection and with 14 cows from an ongoing brucellosis outbreak. All of these animals were slaughtered, and pertinent organs were subjected to classical bacteriological analyses. In addition, we analyzed 23 field cases in which false-positive serological reactions occurred. The IFN-gamma results were compared with those of the standard DTH and a battery of serological assays, and they were correlated with bacteriological data. Both for the experimental infection and for the field brucellosis outbreak, the IFN-gamma assay detected infection in more animals than any combination of the serological tests, and it detected infection earlier than these tests. Finally, none of the samples from cows showing false-positive serological reactions was classified as positive by the IFN-gamma assay, attesting to its specificity and to its usefulness in interpreting ambiguous serological results. A rapid and convenient alternative to the DTH, the IFN-gamma assay appears to be an ideal method that is complementary to the serological diagnosis protocols. [less ▲]

Detailed reference viewed: 28 (4 ULg)
Full Text
Peer Reviewed
See detailExégèse de Paul. D. 39,3 (de aqua et aquae pluviae arcendae), 2,6
Gerkens, Jean-François ULg

in Tijdschrift voor Rechtsgeschiedenis (1995), 63(1995/1), 11-26

Detailed reference viewed: 16 (1 ULg)
Peer Reviewed
See detailSimulation of Square Cup Deep Drawing with Different Finite Elements
Kaiping, Li; Habraken, Anne ULg; Bruneel, H.

in Journal of Materials Processing Technology (1995), 50(1-4), 81-91

Detailed reference viewed: 25 (5 ULg)
See detailHet is nooit goed. Marginalia bij De pasvorm van de goede smaak van Marc Reynebeau
Spinoy, Erik ULg

in Yang : Tijdschrift voor Literatuur en Kommunikatie (1995), 31(3-4), 111-121

This article criticizes the illusions of normative literary criticism, which hopes to determine 'once and for all' the true and objective value of literature.

Detailed reference viewed: 25 (0 ULg)
Peer Reviewed
See detailThe continuum of cytokine involvement in the physiology of reproduction
Geenen, Vincent ULg; Hazee, Marie-Thérèse ULg; Goxe, Béatrice et al

in GENESIS (1995), 14

Detailed reference viewed: 11 (6 ULg)
Peer Reviewed
See detailFamilial trends of obesity through three generations: the Belgian-Luxembourg child study.
Guillaume, Michèle ULg; Lapidus, L.; Beckers, F. et al

in International Journal of Obesity (1995), 19 Suppl 3

Province de Luxembourg in Belgium is an area with clustering of obesity as well as other risk factors for cardiovascular diseases and non-insulin dependent diabetes mellitus. In this study, obesity was ... [more ▼]

Province de Luxembourg in Belgium is an area with clustering of obesity as well as other risk factors for cardiovascular diseases and non-insulin dependent diabetes mellitus. In this study, obesity was studied in a total of 1028 boys and girls in age-strata 6-8, 8-10, 10-12 years, selected at random from school classes. Participation rate was about 70%. Non-participants did not differ from participants in comparisons of school records of height and weight. Furthermore, information on birth weight, parents' height and weight was collected, as well as reported problems of obesity in grandparents. The results show a high prevalence of pronounced obesity in these children. BMIs were strongly correlated between the children and both parents. Furthermore, grandparents' obesity problems were related to the BMI of parents, and also to obesity indices in the children. Birth weights were related to current BMI of the children (in girls only for the youngest age-group), and to their mothers' BMI. It was concluded that obesity is prevalent in this area and can be traced through three generations, and seems to be discoverable already at birth. Energy intake is high and physical activity level is low in these children. However, statistical analyses suggest that familial factors exert a greater impact than environmental factors on the BMI of the children. These results, as well as the wide-spread, consistent familial clustering of obesity, traceable already at birth, suggest influence of strong genetic traits for obesity in this population. [less ▲]

Detailed reference viewed: 23 (2 ULg)
Full Text
Peer Reviewed
See detailNumerical simulation of a full scale fire test on a loaded steel framework
Franssen, Jean-Marc ULg; Cooke, C. M. E.; Latham, D. J.

in Journal of Constructional Steel Research (1995), 35

A single bay single storey steel portal frame has been tested under fire conditions. It is here simulated using hte non linear computer code CEFICOSS. The elements have composite steel-concrete sections ... [more ▼]

A single bay single storey steel portal frame has been tested under fire conditions. It is here simulated using hte non linear computer code CEFICOSS. The elements have composite steel-concrete sections for the thermal analysis, but only the steel part of the sections is load bearing. [less ▲]

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailLate Lower and Middle Devonian Miospores from Saudi Arabia.
Loboziak, S; Streel, Maurice ULg

in Review of Palaeobotany and Palynology (1995), 89

Detailed reference viewed: 5 (0 ULg)
See detailCeci est mon corps. Een inleiding
Spinoy, Erik ULg

in Yang : Tijdschrift voor Literatuur en Kommunikatie (1995), 31(5), 4-10

This is an introduction to a special issue on the body in art and literature.

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailLa gestion en temps réel des opérations relatives à l’exploitation du trafic fluvial
Marchal, Jean ULg; Hage, André ULg; Rodriguez, S.

in Bulletin de l'Association Technique Maritime et Aéronautique (1995)

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailGalectin-3, a Laminin Binding Protein, Fails to Modulate Adhesion of Human Melanoma Cells to Laminin
van den Brule, F. A.; Buicu, C.; Sobel, M. E. et al

in Neoplasma (1995), 42(5), 215-9

Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of ... [more ▼]

Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and A375 melanoma cell expressed galectin-3 on their surface as demonstrated by immunofluorescence, and attached to laminin in an in vitro assay. We demonstrate that neither recombinant galectin-3 nor an affinity purified antigalectin-3 antiserum altered adhesion of A2058 or A375 melanoma cells to laminin. Our data strongly suggest that galectin-3 is not a key element in adhesion of the melanoma cells to laminin. These results are not surprising in light of the observation that galectin-3 expression is down regulated in cancer and that increased adhesion to laminin is a constant feature of invasive cancer cells. [less ▲]

Detailed reference viewed: 34 (1 ULg)
Full Text
Peer Reviewed
See detailCancers épithéliaux de l'ovaire. Acquisitions récentes
van den Brule, F. A.; Lambotte, René ULg; Castronovo, Vincenzo ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1995), 24(3), 241-52

Ovarian carcinomas constitute the major cause of the mortality and morbidity in gynaecology. Most ovary carcinomas are epithelial tumours. Our understanding of ovarian cancerogenesis has been hampered by ... [more ▼]

Ovarian carcinomas constitute the major cause of the mortality and morbidity in gynaecology. Most ovary carcinomas are epithelial tumours. Our understanding of ovarian cancerogenesis has been hampered by the lack of a well defined precursor lesion, the lack of knowledge about tumour progression, and by the relative inaccessibility of the ovaries in the abdominal cavity. Recent studies using experimental models allow us to better define the fundamental mechanisms of carcinogenesis from the serous ovarian cells and of invasion of the abdominopelvic cavity by proximity. This review article tries to update on epidemiology, genetic syndromes, biology, screening, and therapy of these epithelial tumours, and about the new directions taken by basic and clinical research. We will present data concerning oncogenes and tumour suppressor genes involved in epithelial ovarian tumours, regulation of tumour cells by growth factors, genes involved in tumour invasion, and mechanisms used by the cancer cell to resist to therapies. Non-epithelial ovarian tumours will not be examined in this manuscript. [less ▲]

Detailed reference viewed: 35 (2 ULg)
Full Text
Peer Reviewed
See detailProductivité et efficacité dans l'assurance
Fecher-Bourgeois, Fabienne ULg; Pestieau, Pierre ULg

in Risques - Les Cahiers de l'assurance (1995), 22(Avril-juin 1995), 65-71

Detailed reference viewed: 14 (6 ULg)
Full Text
Peer Reviewed
See detailLe méthotrexate en rhumatologie: une révolution thérapeutique
Kaye, O.; Ribbens, Clio ULg; Hauwaert, Christian ULg et al

in Revue Médicale de Liège (1995), 50(11), 453-457

Detailed reference viewed: 75 (1 ULg)
See detailDe Karmelietenkerk in Gent, Dendrochronologische analyse van de dakconstructie boven tweede beuk
Hoffsummer, Patrick ULg; Houbrechts, David

in Stadsarcheologie (1995), 18(2),

Detailed reference viewed: 33 (1 ULg)
Peer Reviewed
See detailComment j'explore... Une anemie (1e partie).
Beguin, Yves ULg

in Revue Médicale de Liège (1995), 50(10), 443-4

Detailed reference viewed: 30 (11 ULg)
Full Text
Peer Reviewed
See detailLow-dose methotrexate: an effective corticosteroid-sparing agent in the musculoskeletal manifestations of sarcoidosis
Kaye, O.; Palazzo, E.; Grossin, M. et al

in British Journal of Rheumatology (1995), 34(7), 642-644

Extrapulmonary sarcoidosis, and particularly the presence of musculoskeletal complications of the disease, may require chronic corticosteroid therapy. In five patients with biopsy-proven sarcoidosis and ... [more ▼]

Extrapulmonary sarcoidosis, and particularly the presence of musculoskeletal complications of the disease, may require chronic corticosteroid therapy. In five patients with biopsy-proven sarcoidosis and presenting recalcitrant forms of the disease, we introduced low-dose oral methotrexate (MTX) [10 mg/week (7.5-15)] for 30 months (16-34) to control the clinical and biological symptoms as well as to try to reduce the intolerated steroid posology. Beneficial effects were observed within 8-12 weeks in all patients, which allowed a reduction of 59% (35-75) of the corticosteroid posology, and maintained with a follow-up of 3 yr in 4/5 patients. No significant toxicity was observed. MTX appears to be an efficient, safe and corticosteroid-sparing therapeutic agent for the treatment of recalcitrant musculoskeletal manifestations of sarcoidosis. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailCharacteristics of the pulsatile liberation of LH in Italian Simmental cows from the dry period to early lactation
Prandi, Alberto; Motta, Mario; Rossi, A. et al

in Bulletin des Recherches Agronomiques de Gembloux (1995), 30

Detailed reference viewed: 14 (0 ULg)
Peer Reviewed
See detailNF kappa B and interferon regulatory factor 1 physically interact and synergistically induce major histocompatibility class I gene expression.
Drew, P. D.; Franzoso, G.; Becker, K. G. et al

in Journal of Interferon & Cytokine Research (1995), 15(12), 1037-45

Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has ... [more ▼]

Major histocompatibility (MHC) class I gene expression is synergistically induced by the cytokines TNF-alpha and IFN-gamma. However, the mechanism that results in synergistic activation of these genes has remained unclear. We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence (ICS). We further demonstrated that NF kappa B and IRF-1 physically interacted with each other and cooperatively induced MHC class I gene expression when cotransfected into CHP-126 neuroblastomas. These results provide a molecular mechanism by which TNF-alpha and IFN-gamma synergistically induce the expression of a variety of genes involved in immune responses, including MHC class I. [less ▲]

Detailed reference viewed: 15 (1 ULg)
See detailIn vivo responsiveness of morphological variants of growth hormone-producing pituitary adenomas to octreotide
Ezzat, S.; Kontogeorgos, G.; Redelmeier, D. A. et al

in European Journal of Endocrinology (1995), 133

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailLaparoscopic Intrauterine Insemination in the Bitch
Silva, L. D.; Onclin, K.; Snaps, Frédéric ULg et al

in Theriogenology (1995), 43(3), 615-23

A technique for laparoscopic intrauterine insemination in bitches is described. During natural estrus, 5 beagle bitches were inseminated and S others were naturally mated (control group) twice at a 48-h ... [more ▼]

A technique for laparoscopic intrauterine insemination in bitches is described. During natural estrus, 5 beagle bitches were inseminated and S others were naturally mated (control group) twice at a 48-h interval on Days 3 and S (n = 4) or Days 4 and 6 (n = 6) after the increase in plasma progesterone considered to be indicative of the day of the preovulatory LH peak. All the inseminations were with fresh semen and under general anesthesia. The technique involved the introductions of 1) a Verres needle to insufflate the abdominal cavity by direct punction on the middle line 1 cm over the umbilicus, 2) a laparoscope to visualize the abdominal cavity by a 1 cm puncture on the middle line 1 cm under the umbilicus, 3) a forceps used to manipulate the uterus by a 0.5 cm puncture at 2 to 3 cm lateral to the mammary glands, and 4) an 18-g catheter used to puncture the uterus on the middle line between the 3rd and 5th mammary gland. The uterine body was grasped by the forceps and elevated against the ventral abdominal wall. The 18-g catheter was then inserted through the abdominal wall directly into the uterine lumen, and 1.0 ml of fresh semen containing 250 to 480 x 10(6) spermatozoa/ml was injected. The inseminations resulted in pregnancies in all animals. Litter size was similar in the artificially inseminated and naturally mated bitches (5 +/- 1.8 and 4.8 +/- 1.6 pups per litter, respectively). Bitches in the artificially inseminated group delivered at 65.2 +/- 0.8 d and in the natural mated group at 65.4 +/- 0.5 d after the LH peak. In conclusion, this paper gives the first results of intrauterine laparoscopic insemination in bitches, indicating interesting perspectives for this technique in dog's reproduction. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailA Propos de la Physique du Ruissellement sur Bassin Versant
Pirotton, Michel ULg

in Tribune de l'Eau (La) (1995), 48(573), 3-10

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailExtracellular matrix proteins and basement membrane identification in bovine ovaries and significance for the attachment of cultured preantral follicles
Figueiredo, J. R.; Hulshof, S. C. J.; Thiry, Marc ULg et al

in Theriogenology (1995), 43(5), 845-858

Described in the present paper is the immunolocalization of the extracellular matrix proteins (e.g., fibronectin, collagen Types I and III) in the bovine ovary, with special attention to preantral ... [more ▼]

Described in the present paper is the immunolocalization of the extracellular matrix proteins (e.g., fibronectin, collagen Types I and III) in the bovine ovary, with special attention to preantral follicles. In addition, we have shown, histochemically and ultrastructurally, that mechanically isolated bovine preantral follicles are surrounded by an intact basement membrane. After 24 h of culture in serum-free medium, only 20.4% of these follicles attached to a plastic substrate. We showed that covering the plastic with extracellular matrix proteins (i.e., fibronectin, collagen Type I and matrigel) significantly increased the percentage of attached follicles to 76.0, 65.2 and 80.4%, respectively, while laminin had no effect (18.6%). When preantral follicles were embedded within three-dimensional collagen gels, no loss of follicles was observed. Restoring surface interactions between preantral follicles and the extracellular matrix in vitro, either in a two- or a three-dimensional system, might be important for maintaining follicular viability and growth in the future. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailThe pancreatic islet factor STF-1 binds cooperatively with Pbx to a regulatory element in the somatostatin promoter: importance of the FPWMK motif and of the homeodomain.
Peers, Bernard ULg; Sharma, S.; Johnson, T. et al

in Molecular & Cellular Biology (1995), 15(12), 7091-7

A number of homeodomain proteins have been shown to regulate cellular development by stimulating the transcription of specific target genes. In contrast to their distinct activities in vivo, however, most ... [more ▼]

A number of homeodomain proteins have been shown to regulate cellular development by stimulating the transcription of specific target genes. In contrast to their distinct activities in vivo, however, most homeodomain proteins bind indiscriminately to potential target sites in vitro, suggesting the involvement of cofactors which specify target site selection. One such cofactor, termed extradenticle, has been shown to influence segmental morphogenesis in Drosophila melanogaster by binding cooperatively with certain homeodomain proteins to target regulatory elements. Here we demonstrate that STF-1, an orphan homeodomain protein required for pancreatic development in mammals, binds cooperatively to DNA with Pbx, the mammalian homolog of extradenticle. Cooperative binding with Pbx requires a pentapeptide motif (FPWMK) which is well conserved among a large subset of homeodomain proteins. The FPMWK motif is not sufficient to confer Pbx cooperativity on other homeodomain proteins, however; the N-terminal arm of the STF-1 homeodomain is also essential. As cooperative binding with Pbx occurs on only a subset of potential STF-1 target sites, our results suggest that Pbx may specify target gene selection in the developing pancreas by forming heterodimeric complexes with STF-1. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailExperimental production of hypocalcemia by EDTA infusion in calves : a critical appraisal assessed from the profile of blood chemicals and enzymes
Desmecht, Daniel ULg; Linden, Annick ULg; Godeau, J. M. et al

in Comparative Biochemistry and Physiology. A : Comparative Physiology (1995), 110A(2), 115-130

Detailed reference viewed: 25 (0 ULg)
Peer Reviewed
See detailAssessment of equine diaphragm strength and activation using transvenous phrenic nerve stimulation
Desmecht, Daniel ULg; Linden, Annick ULg; Lekeux, Pierre ULg

in Equine Veterinary Journal. Supplement (1995), 18

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailBiology and clinical uses of erythropoietin in infants and children
Beguin, Yves ULg

in International Journal of Pediatric Hematology/Oncology (1995), 2

Detailed reference viewed: 17 (5 ULg)
See detailCharles Grandgagnage (Liège 1812-Liège 1878)
Boutier, Marie-Guy ULg

in Wallonnes : Chronique de la Société de Langue et de Littérature Wallonnes (1995), (4), 4

Detailed reference viewed: 33 (0 ULg)
Full Text
Peer Reviewed
See detailTEM1 beta-lactamase structure solved by molecular replacement and refined structure of the S235A mutant.
Fonze, E.; Charlier, Paulette ULg; To'th, Y. et al

in Acta Crystallographica Section D-Biological Crystallography (1995), 51(Pt 5), 682-94

beta-Lactamases are bacterial enzymes which catalyse the hydrolysis of the beta-lactam ring of penicillins, cephalosporins and related compounds, thus inactivating these antibiotics. The crystal structure ... [more ▼]

beta-Lactamases are bacterial enzymes which catalyse the hydrolysis of the beta-lactam ring of penicillins, cephalosporins and related compounds, thus inactivating these antibiotics. The crystal structure of the TEM1 beta-lactamase has been determined at 1.9 A resolution by the molecular-replacement method, using the atomic coordinates of two homologous beta-lactamase refined structures which show about 36% strict identity in their amino-acid sequences and 1.96 A r.m.s. deviation between equivalent Calpha atoms. The TEM1 enzyme crystallizes in space group P2(1)2(1)2(1) and there is one molecule per asymmetric unit. The structure was refined by simulated annealing to an R-factor of 15.6% for 15 086 reflections with I >/= 2sigma(I) in the resolution range 5.0-1.9 A. The final crystallographic structure contains 263 amino-acid residues, one sulfate anion in the catalytic cleft and 135 water molecules per asymmetric unit. The folding is very similar to that of the other known class A beta-lactamases. It consists of two domains, the first is formed by a five-stranded beta-sheet covered by three alpha-helices on one face and one alpha-helix on the other, the second domain contains mainly alpha-helices. The catalytic cleft is located at the interface between the two domains. We also report the crystallographic study of the TEM S235A mutant. This mutation of an active-site residue specifically decreases the acylation rate of cephalosporins. This TEM S235A mutant crystallizes under the same conditions as the wild-type protein and its structure was refined at 2.0 A resolution with an R value of 17.6%. The major modification is the appearance of a water molecule near the mutated residue, which is incompatible with the OG 235 present in the wild-type enzyme, and causes very small perturbations in the interaction network in the active site. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detail11-(4-methylpiperazin-1-yl)-5H-pyrido[4,3-b][1,5]benzodiazepine
Dupont, L.; Liégeois, Jean-François ULg; Rogister, F. et al

in Acta Crystallographica Section C-Crystal Structure Communications (1995), C51

Detailed reference viewed: 48 (4 ULg)
Peer Reviewed
See detailLe determinisme genetique de la differenciation sexuelle humaine.
Ectors, Fabien ULg

in Revue Médicale de Bruxelles (1995), 16(6), 404-10

The genetic mechanism controlling sexual differentiation had remained unknown for a long time. Karyotype analysis of sex-inverted patients or individuals with ambiguous sexual differentiation has enabled ... [more ▼]

The genetic mechanism controlling sexual differentiation had remained unknown for a long time. Karyotype analysis of sex-inverted patients or individuals with ambiguous sexual differentiation has enabled the localization and identification of genes involved. It is currently known that the SRY gene is responsible for the initiation of a cascade reaction leading to male differentiation of the primitive gonad. SRY is a +/- 820 base pairs gene located on the small arm of the Y chromosome, more precisely within the 1A1 alpha sub-segment. Although a few other genes are known to be involved in the downstream regulation of SRY, their precise mode of action is yet unknown. [less ▲]

Detailed reference viewed: 33 (0 ULg)
Peer Reviewed
See detailAmylin/islet amyloid polypeptide: biochemistry, physiology, patho-physiology.
Castillo, M. J.; Scheen, André ULg; Lefebvre, Pierre ULg

in Diabète & Métabolisme (1995), 21(1), 3-25

Amylin is a 37 amino-acid peptide mainly produced by the islet beta-cell. Aggregation of amylin is partly responsible for amyloid formation. Amyloid deposits occur both extracellularly and intracellularly ... [more ▼]

Amylin is a 37 amino-acid peptide mainly produced by the islet beta-cell. Aggregation of amylin is partly responsible for amyloid formation. Amyloid deposits occur both extracellularly and intracellularly and may contribute to beta-cell degeneration. Amylin is packed in beta-cell granules and cosecreted with insulin in response to the same stimuli but, unlike other beta-cell products, it is produced from specific a gene on chromosome 12. Basal, plasma amylin concentrations are around 5 pM, and increase fourfold after meals or glucose. Higher levels are found in cases of insulin resistance, obesity, gestational diabetes and in some patients with NIDDM. Low or absent levels are found in insulin-dependent diabetic patients. There are similarities between amylin and non beta-cell peptides such as calcitonin gene related peptides (CGRP). They may bind to the same receptor, determine similar post-receptor phenomena and qualitatively similar actions but with different degree of potency. The actions of amylin are multiple and mostly exerted in the regulation of fuel metabolism. In muscle, amylin opposes glycogen synthesis, activates glycogenolysis and glycolysis (increasing lactate production). Consequently, amylin increases lactate output by muscle and increases the plasma lactate concentration. In fasting conditions, this lactate may serve as a gluconeogenic substrate for the liver, contributing to replenish depleted glycogen stores and to increase glucose production. In non-fasting conditions, lactate can be transformed by liver in triglycerides. It is not clear at present whether amylin actions on the liver are direct or mediated by changes in circulating metabolites. A probably indirect effect of amylin in muscle is to decrease insulin- (or glucose)-induced glucose uptake, which may contribute to insulin resistance. Other actions include inhibition of glucose-stimulated insulin secretion and, in general, actions mimicking CGRP effects. Some of these actions are seen at supraphysiological concentrations. The physiopathological consequences of amylin deficiency, or excess are under active by investigated. [less ▲]

Detailed reference viewed: 38 (1 ULg)
Full Text
Peer Reviewed
See detailMeasurement of glucosinolates in rapeseeds.
Wathelet, Jean-Paul ULg; Marlier, M.; Severin, M. et al

in Natural toxins (1995), 3(4),

Several ring tests have been organized by the International Organisotion for Standardization (lSO) and the Measurement ônd Testing Programme (BCR) for improving glucosinolote methods Finally, HPLC of ... [more ▼]

Several ring tests have been organized by the International Organisotion for Standardization (lSO) and the Measurement ônd Testing Programme (BCR) for improving glucosinolote methods Finally, HPLC of desulphoglucosinolates is recommended by lSO, the European Committee for Standardizotion (CEN), and the European Commission (EC) as the official method. X-ray fluorescence has also become particularly common for fast analysis. After checking stability of intact glucosinolates in whole rapeseeds, the BCR has three reference materials (CRM 366, CRM 190, CRM 367) avai able, certified for their total glucosinolate and sulphur contents. Currently, the Measurement and Testing Programme is supporting a research project concerning the stdbility of desulphoglucosinolates and intact glucosinolates extracts. At the present time, best results were observed with lyophilized extracts sealed in brown glass ampoules and stored at 18'C. [less ▲]

Detailed reference viewed: 24 (1 ULg)
Peer Reviewed
See detailSoya oil in diet for growing-fattening bulls: I. Effects on animal performances and carcass composition
Clinquart, Antoine ULg; Van Eenaeme, Christian ULg; Dufrasne, Isabelle ULg et al

in Journal of Animal Physiology & Animal Nutrition (1995), 74

Detailed reference viewed: 3 (0 ULg)