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See detailInhibition of growth of normal and human papillomavirus-transformed keratinocytes in monolayer and organotypic cultures by interferon-gamma and tumor necrosis factor-alpha.
Delvenne, Philippe ULg; al-Saleh, W.; Gilles, Christine ULg et al

in American Journal of Pathology (1995), 146(3), 589-98

The growth response of normal and human papillomavirus (HPV)-transformed cervical keratinocytes to interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha was investigated in monolayer and ... [more ▼]

The growth response of normal and human papillomavirus (HPV)-transformed cervical keratinocytes to interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha was investigated in monolayer and organotypic raft cultures. The proliferation rates of monolayer cultures were assessed by [3H]TdR incorporation and fluorimetric DNA titration. The growth of keratinocytes in organotypic cultures was estimated by their ability to stratify on collagen rafts and by immunohistochemistry for Ki67 antigen expression. IFN-gamma reduced the DNA synthesis of normal and HPV-transformed keratinocytes in monolayer cultures and exerted a marked growth inhibitory effect in organotypic raft cultures. In control raft cultures, normal keratinocytes produced an epithelial sheet of approximately 10 cells in thickness that closely resembled normal cervical epithelium and was characterized by sparse Ki67 antigen-positive cells whereas HPV-transformed keratinocytes produced up to 15 poorly differentiated epithelial layers that were reminiscent of high grade cervical lesions seen in vivo and exhibited a full thickness Ki67 antigen expression. When normal and HPV-transformed keratinocytes were maintained in the presence of IFN-gamma, the epithelial sheet was reduced to a few cells in thickness and the density of Ki67 antigen-positive cells was decreased. A more pronounced growth inhibitory effect in monolayer and organotypic cultures was observed when IFN-gamma was associated with tumor necrosis factor-alpha Tumor necrosis factor-alpha alone reduced the DNA synthesis of normal keratinocytes but was significantly less effective than IFN-gamma to inhibit the growth of HPV-transformed keratinocytes. These results suggest that similar responses in vivo to regulatory molecules may play a role in the development of HPV-related lesions. [less ▲]

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See detailGlucose handling, diabetes and ageing.
Paolisso, G.; Scheen, André ULg; Lefebvre, Pierre ULg

in Hormone Research (1995), 43(1-3), 52-7

The relationship between ageing and glucose homeostasis is still an open debate. In fact, the mechanisms by which glucose metabolism is progressively impaired with increasing age are not completely ... [more ▼]

The relationship between ageing and glucose homeostasis is still an open debate. In fact, the mechanisms by which glucose metabolism is progressively impaired with increasing age are not completely understood. In the present report we have reviewed the possible mechanisms (impaired insulin secretion and action, role of the environmental factors) which may lead to the impairment in glucose handling associated with ageing. We also point out that not all aged subjects are glucose intolerant; in fact, it has been suggested that only those aged subjects who present more than one pathological finding do in fact develop impaired glucose handling. [less ▲]

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See detailAnalysis of the open reading frames of the main capsid proteins of actinophage VWB.
Anne, J; Fiten, P; Van Mellaert, L et al

in Archives of Virology (1995), 140(6), 1033-47

The nucleotide sequence of a 6 kb fragment encoding the main late proteins (p14, p38 and p24) of actinophage VWB was obtained. Sequence comparison of the encoded proteins with those filed in databases ... [more ▼]

The nucleotide sequence of a 6 kb fragment encoding the main late proteins (p14, p38 and p24) of actinophage VWB was obtained. Sequence comparison of the encoded proteins with those filed in databases indicated that the phage VWB main late proteins were all novel. A search for special motifs revealed that p14 (13.3 kDa) has a P-loop sequence commonly found in ATP- and GTP-binding proteins. This observation might indicate that p14 is important for ATP-driven DNA translocation during encapsidation of VWB phage DNA into the phage head. Furthermore, the polypeptide ORF2 (26.9 kDa) has an unusual primary structure consisting of 3 stretches of acidic amino acid residues and a glycine/arginine rich C-terminal end. From comparison with other proteins including the bacteriophage T4 prohead core component and from the data of special motif analysis the ORF2 gene product is probably involved in prohead core formation. [less ▲]

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See detailA biotechnological process to produce sulfide from the industrial by-product gypsum : new investigations
Hiligsmann, Serge ULg; Taillieu, Xavier; Thonart, Philippe ULg

in Med. Fac. Landbouww. Univ. Gent 60/4b (1995)

Different methods to isolate acetate-oxidizing sulfate-reducing bacteria (SRB) have been applied to samples containing relatively high concentrations of sulfate. However, the presence of facultative ... [more ▼]

Different methods to isolate acetate-oxidizing sulfate-reducing bacteria (SRB) have been applied to samples containing relatively high concentrations of sulfate. However, the presence of facultative anaerobic bacteria, identified as enterobacteriaceae, did not allow to obtain pure strains. Consequently, the influence of this contaminant in SRB cultures was investigated with particular attention being paid to the gases produced by the contaminant. [less ▲]

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See detailAzidothymidine as a potential modifier of radiation-reponse
Copaceanu, M-L; COUCKE, Philippe ULg; Cottin, E et al

in Acta Oncologica (1995)

Abstract The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 μM AZT on exponentially ... [more ▼]

Abstract The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 μM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation. [less ▲]

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See detailCharacterization of monoclonal antibodies against stromelysin-3 and their use to evaluate stromelysin-3 levels in breast carcinoma by semi-quantitative immunohistochemistry
Santavicca, M.; Noël, Agnès ULg; Chenard, M. P. et al

in International Journal of Cancer = Journal International du Cancer (1995), 64(5), 336-341

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ... [more ▼]

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ST3 forms in Escherichia coli from cDNA constructs indicated that high levels of expression were achieved when the ST3 pro-domain was deleted. The putative mature form of ST3 thus produced and recovered from bacterial inclusion bodies was used to prepare monoclonal antibodies (MAbs) against ST3 by immunization of BALB/C mice. Ten hybridomas producing MAbs against ST3 were obtained and analyzed for their ability to detect endogenous ST3 in breast cancer and in conditioned media from human fibroblasts. One of these MAbs (5ST-4A9) was found to be suitable for the routine detection of ST3 on breast cancer tissue sections, thus opening the possibility to evaluate ST3 prognostic value in breast cancer using semi-quantitative immunohistochemistry [less ▲]

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See detailNotice sur Marcel Migeotte
Biémont, Emile ULg

in Annuaire de l'Académie Royale de Belgique (1995)

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See detailLack of LTR and ENV genetic variation during bovine leukemia virus-induced leukemogenesis.
Willems, Luc ULg; Kerkhofs, P.; Burny, A. et al

in Virology (1995), 206(1),

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See detailAntipsychotics and Neuropeptides: The Atypical Profile of Ci-943 and Its Relationship to Neurotensin
Liégeois, Jean-François ULg; Bonaventure, P.; Delarge, J. et al

in Neuroscience & Biobehavioral Reviews (1995), 19(4, Winter), 519-31

CI-943 is a new drug candidate with antipsychotic-like activity in a variety of behavioural tests in rodents and primates, but without any affinity for brain dopamine receptors. CI-943 does not cause ... [more ▼]

CI-943 is a new drug candidate with antipsychotic-like activity in a variety of behavioural tests in rodents and primates, but without any affinity for brain dopamine receptors. CI-943 does not cause dystonia in monkeys, a predictive symptom of extrapyramidal side effects (EPS). Its mechanism of action remains unclear. Neurotensin (NT) concentration in nucleus accumbens and caudate is increased by CI-943; this may be associated with its antipsychotic effect. Indeed various observations suggest that the clinical action of antipsychotic drugs may at least be partially mediated by some neuropeptides. Various actions of neurotensin are reviewed. The hypothesis on the role of neurotensin represents a new strategy in the development of pharmacological tools for the treatment of schizophrenia. [less ▲]

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See detailBiological activity and immunological reactivity of human prolactin mutants
Rhee, Harrison K; Sun, Zhenyu; Kim, Steve S et al

in Endocrinology (1995), 136(11), 4990-5

We examined the biological activity and immunological reactivity of four mutants of human PRL. Two were mutants that changed the ability of human PRL to inhibit rat PRL storage when transfected into a rat ... [more ▼]

We examined the biological activity and immunological reactivity of four mutants of human PRL. Two were mutants that changed the ability of human PRL to inhibit rat PRL storage when transfected into a rat pituitary cell line:mutations S34A and N31T. Two mutations were in regions of PRL that are highly conserved. One, des(3-11)-PRL, removed the N-terminal cystine loop that most PRLs, except those from certain fish, have, and no GHs have. The other, S90A, mutated a serine that is present in all PRLs but those from some fish and in all GHs. The immunological properties of des(3-11)-PRL were reduced 10-fold compared to those of wildtype human PRL in a RIA using NIH antihuman PRL-3, AFP C11580; the others were similar to those of wild-type PRL. The biological activity of des(3-11)-PRL was the most affected; activity was reduced about 8-fold compared to that of wild-type PRL in the Nb2 cell assay. The activities of the others were similar to that of the wild type. Serine 90 may be partially buried by loops connecting the alpha-helixes. The mutation of serine 90 did not affect the stability of the molecule in vitro, determined by comparing the red shift in tryptophan fluorescence that occurs with increasing concentrations of urea in S90A and wild-type PRL. The activity of S34A and N31T mutations indicates there is no correlation between biological activity and ability to affect storage. The N-terminal cystine loop may be conserved because it is needed for biological activity, but the conservation of serine 90 in GH and PRL must be determined by other properties, such as spacial requirements. [less ▲]

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See detailLASH: laparoscopic supracervical (subtotal) hysterectomy
Donnez, Jacques; Smets, Mireille; Polet, Roland et al

in Zentralblatt für Gynakologie (1995), 117(12), 629-32

From July 1990 to December 1993, 156 laparoscopic supracervical hysterectomies (LASH) were performed. No complications occurred. The technique and the advantages are described. Because of the easy ... [more ▼]

From July 1990 to December 1993, 156 laparoscopic supracervical hysterectomies (LASH) were performed. No complications occurred. The technique and the advantages are described. Because of the easy feasibility of this technique, the quick recovery of the patients and the absence of complications this strictly laparoscopic approach must be widely proposed when an hysterectomy is indicated and no risk of remaining cervix cancer is assumed. [less ▲]

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See detailDifferential effects of drawing and indirect dopamine agonists on the induction of gnawing in C57BI/6J mice
Tirelli, Ezio ULg; Witkin, J. M.

in Journal of Pharmacology and Experimental Therapeutics (The) (1995), 273(1), 7-15

Compared the ability of indirect dopamine (DA) agonists to induce gnawing behavior (GB) in male C57BL/6J mice with that of direct DA agonists acting at DA D-sub-1 or D-sub-2 receptor subtypes. Eight Ss ... [more ▼]

Compared the ability of indirect dopamine (DA) agonists to induce gnawing behavior (GB) in male C57BL/6J mice with that of direct DA agonists acting at DA D-sub-1 or D-sub-2 receptor subtypes. Eight Ss were used per dose. Holes left by Ss on corrugations of packing cardboard were used as an objective index of GB. Indirect DA agonists, including DA releasers such as fencamfamine and amfonelic acid and DA uptake inhibitors such as cocaine and nomifensine, produced dose-dependent increases in GB. None of the direct agonists (e.g., apomorphine, quinpirole) increased GB. The dopaminergic nature of GB was confirmed in studies in which a host of compounds (e.g., nicotine, caffeine, dizocilpine) with primary actions at nondopaminergic sites did not induce GB. Given the general contrast between the effects of direct and indirect DA agonists, this procedure could serve as a rapid in vivo method of distinguishing direct- from indirect-acting DA agonists. ((c) 1997 APA/PsycINFO, all rights reserved) [less ▲]

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See detailSurgical Technique for Reinforcement Cardiomyoplasty
RADERMECKER, Marc ULg; Chachques, J.C.; Fourny, J. et al

in Acta Chirurgica Belgica (1995), 95(6), 271-4

The current technique for reinforcement cardiomyoplasty in man is detailed. The authors emphasize the critical points that determine the perfect execution of this operation. This innovative surgical ... [more ▼]

The current technique for reinforcement cardiomyoplasty in man is detailed. The authors emphasize the critical points that determine the perfect execution of this operation. This innovative surgical approach to heart failure is continuously evolving and, therefore, may be subject to improvement. The major principles however remain valuable. [less ▲]

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See detailDebate. Personal Experience and Research Experience : A Reflexive Account
Martiniello, Marco ULg; Ouali, Nouria; Choenni, C.

in Merger (1995), 3(2), 4-7

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See detailThe hydrophobic effect in protein folding.
Lins, Laurence ULg; Brasseur, Robert ULg

in FASEB Journal (1995), 9(7), 535-40

In this review of protein folding we consider the noncovalent interactions existing between atoms or molecules at the molecular level. The electrostatic, Van der Waals, hydrogen bonding, and hydrophobic ... [more ▼]

In this review of protein folding we consider the noncovalent interactions existing between atoms or molecules at the molecular level. The electrostatic, Van der Waals, hydrogen bonding, and hydrophobic interactions are described and their contribution to protein conformation is discussed. The growing interest in the hydrophobic effect arises from its importance in the protein folding process, and a semiempirical simulation of the free energy of solvation is proposed. In most proteins, the different forces we describe contribute to the stability of the protein conformation in a complex way. However, in the case of the apolipoproteins and cytochrome C551, the energetic contributions are easily distinguished. For this reason, these proteins are used to illustrate the importance of the different energy fields. [less ▲]

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See detailNF-kappaB transcription factor and human immunodeficiency virus type 1 (HIV-1) are activated by methylene blue photosensitization
Piret, Bernard; Legrand-Poels, Sylvie ULg; Sappey, Christine et al

in European Journal of Biochemistry (1995)

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See detailLes activateurs de canaux potassiques: étude structurale comparative du pinacidil, du diazoxide et du cromakalim
Dupont, Léon ULg; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Annales Pharmaceutiques Françaises (1995), 53(5), 201-208

A conformational analysis has been performed with SYBYL starting from the energy optimized (Tripos force field) X-ray conformation of (R,S)-pinacidil. The geometry of four selected low energy conformers ... [more ▼]

A conformational analysis has been performed with SYBYL starting from the energy optimized (Tripos force field) X-ray conformation of (R,S)-pinacidil. The geometry of four selected low energy conformers has been reoptimized using the AM1 semiempirical Molecular Orbital method (MOPAC 5.0), and the total energy of the optimized conformers has been compared. In spite of an apparent structural dissimilarity, a good analogy has been found between the calculated isopotential map of diazoxide and that of at least one selected low energy conformer of pinacidil. It has been suggested that, unlike cromakalim, the low but observable activity of pinacidil on pancreatic B-cells could be explained by adoption for this compound of a conformation having a diazoxide-like stereoelectronical imprint which could assume a similar interaction on the same biological receptor. [less ▲]

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See detailEffect of high osmolarity acclimation on tolerance to hyperosmotic shocks in L929 cultured cells.
Gilles, Raymond ULg; Belkhir, M.; Compère, Philippe ULg et al

in Tissue & Cell (1995), 27(6), 679-687

Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin ... [more ▼]

Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin. This paper shows that; 1) this phenomenon is concomitant with a complete disappearance of deoxyribonucleic acid, as visualized by immunogold labelling, from the nucleoplasmic spaces; 2) acclimation to high osmolarities (600 mOsm) by addition to the culture medium of NaCl, sorbitol or proline protects the cells from these effects, which appear to be largely attenuated-acclimated cells also survive much better to the osmotic shock than do control cells and; 3) the best protection seems to be provided by sorbitol and NaCl. Proline acclimation is less effective. These effects are discussed in terms of increased tolerance to NaCl load induced at the level of different macromolecules by so-called 'compensatory' organic compounds. [less ▲]

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See detailInfluence du risque suicidaire et du desespoir sur l'amplitude de l'onde P300 dans la depression majeure.
Urcelay-Zaldua, I.; Hansenne, Michel ULg; Ansseau, Marc ULg

in Neurophysiologie Clinique = Clinical Neurophysiology (1995), 25(5), 291-6

P300 amplitude represents a useful tool to assess information processing in normal and psychopathological subjects. In depressive disorders, many studies have shown a decrease of P300 amplitude and an ... [more ▼]

P300 amplitude represents a useful tool to assess information processing in normal and psychopathological subjects. In depressive disorders, many studies have shown a decrease of P300 amplitude and an increase of its latency. However, functional significance of the P300 modifications remain unclear. The aim of the study was to assess the influence of suicidal risk and hopelessness on P300 amplitude among 40 depressive inpatients. The results showed significant relationships between P300 amplitude and suicidal risk (r = -0.68, p < 0.001) and with hopelessness (r = -0.76, p < 0.001). From a clinical point of view, P300 amplitude should be considered as a psychophysiological index of suicidal risk in major depressive disorder. [less ▲]

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See detailReactivity and Collisionally Activated Dichlorocarbene Dications studied by Tandem Mass Spectrometry.
Leyh, Bernard ULg; Hautot, D.

in Journal of the American Society for Mass Spectrometry (1995), 6

The dissociation mechanisms of dichlorocarbene dications following collisional activation have been investigated via tandem mass spectrometric technique and semi-empirical calculations. Three channels ... [more ▼]

The dissociation mechanisms of dichlorocarbene dications following collisional activation have been investigated via tandem mass spectrometric technique and semi-empirical calculations. Three channels appear to be significant: (CCl2)2+->CCl+(X1Sigma+)+Cl+(3P) (channel 1); (CCl2)2+->CCl+(X1Sigma+)+Cl+(1D) (channel 2); (CCl2)2+->(Cl2)+(X2Pig)+C+(2P). The second channel becomes dominant at high internal energy. Production of ground state fragments (channel 1) involves a transition driven by spin-orbit coupling from the (CCl2)2+ X 1Sigma+g state to the (CCl2)2+ a 3Sigma-u state en route to the fragments. The dissociation barrier for the production of the ground state fragments from the ground electronic state of (CCl2)2+ via the spin-orbit-induced transition is equal to 420 kJ/mol. The dissociation pathway that corresponds to channel 3 includes a first isomerization step from the linear (Cl-C-Cl)2+ structure to a bent (Cl-C-Cl)2+ connectivity. The calculated isomerization barrier amounts to 550 kJ/mol. The calculated reverse activation barriers are compatible with the measured kinetic energy released on the fragments. [less ▲]

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See detailHelix Helix Interactions In Reconstituted High-Density-Lipoproteins
Lins, Laurence ULg; Brasseur, Robert ULg; De Pauw, Mc. et al

in Biochimica et Biophysica Acta-Lipids and Lipid Metabolism (1995), 1258(1), 10-8

In this work we calculated the ionic interactions between adjacent amphipathic helices of apo A-I and apo A-IV. The calculation of the electrostatic potential around the helices helps identify the charged ... [more ▼]

In this work we calculated the ionic interactions between adjacent amphipathic helices of apo A-I and apo A-IV. The calculation of the electrostatic potential around the helices helps identify the charged residues susceptible to form salt bridges between adjacent helices. An estimation of the stability of the different pairs of helices is derived from the calculation of the energy of interaction between contiguous helices at a water/lipid interface after energy minimization. The most stable energetic conformation corresponds to the 17-residue helices oriented anti-parallel and separated by a stretch of 5 residues in an extended beta-strand conformation, as calculated through the 'stereo alphabet' calculation procedure. In a pair of helices, the hydrophobic faces are directed towards the lipid core of the discoidal phospholipid-apolipoprotein complex and the hydrophobic lipid-protein interactions are major determinants for the stability of the complex. Interactions between polar residues located on the opposite face of the helix and water molecules can also contribute to the overall energy of the system. Finally, salt bridge formation between residues of opposite charge along the edge of the helical segments contribute to the cooperativity of the phospholipid-apolipoprotein complex formation. The mode of assembly of the amphipathic helical repeats of the apolipoproteins around the edge of a discoidal complex is therefore determined both by the hydrophobic character of the residues and by the charge complementarity along the edge of the helices which increases the structural stability and determines the relative orientation of the helices. [less ▲]

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See detailLogica en esthetica. Notities bij ‘Het bordeel van Ika Loch’ van Paul van Ostaijen
Spinoy, Erik ULg

in Spiegel der Letteren (1995), 37(2-3), 89-128

This article is an innovative rereading of Van Ostaijen's grotesque story 'Ikla Loch's Brothel', in light of the author's late philosophical views.

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See detailLumière und der Augen-Blick
Belloi, Livio ULg

in KINtop : Jahrbuch zur Erforschung des frühen Films (1995), 4

Analyse de la pratique de la vue chez Lumière, envisagée dans sa double dimension spatiale et temporelle.

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See detailEffect of risedronate on collagen cross-links in postmenopausal osteoporosis
Zegels, Brigitte ULg; Balena, R; Eastell, R et al

in Journal of Bone and Mineral Research (1995), 10(s1), 455

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See detailInhaled nitric oxide for hemodynamic support after postpneumectomy ARDS
Chiche, Jean-Daniel; Canivet, Jean-Luc ULg; Damas, Pierre ULg et al

in Intensive Care Medicine (1995), 21(8), 675-678

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See detailAnalysis of Liquid Flow Distribution in Trickling Flow Reactor Using Computer-Assisted X-Ray Tomography
Toye, Dominique ULg; Marchot, Pierre ULg; Crine, Michel ULg et al

in Chemical Engineering Research & Design : Transactions of the Institution of Chemical Engineers Part A (1995), 73(A3), 258-262

In this paper, we propose a new experimental technique, based on X-ray tomography, allowing the analysis of the gas-liquid-solid phase distribution in trickling flow reactors. Tomography refers to the ... [more ▼]

In this paper, we propose a new experimental technique, based on X-ray tomography, allowing the analysis of the gas-liquid-solid phase distribution in trickling flow reactors. Tomography refers to the cross-sectional imaging of an object from data collected by illuminating this object from many directions. The experimental set-up allows the scanning of transverse sections of rather large columns: up to 0.8 m diameter and 2 m height can be irradiated. Experiments were carried out both without and with liquid flow. In the absence of liquid, the reconstructed images give a view of the non-uniformity of the solid phase distribution. The images obtained are very complex because they result from the superposition of several distributions with different Spatial scales. We show the existence of a first spatial scale which allows us to separate properties belonging to the individual packing elements from those belonging to the bed scale. When liquid is flowing through the packed bed X-ray scans give a clear picture of the distribution of the liquid phase saturation, despite the very low liquid film thickness. Average values of this liquid phase saturation are determined and confronted with a partial wetting model. [less ▲]

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See detailFeed restriction and IGFBP in bulls: effects of increasing starving period lengths.
Massart, Serge; Van Eenaeme, Christian ULg; Renaville, Robert ULg et al

in Journal of Animal Science (1995), 73(suppl 1), 225

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See detailRecommendations for the registration of new chemical entities used in the prevention and treatment of osteoporosis
Reginster, Jean-Yves ULg; Compston, JE; Jones, EA et al

in Calcified Tissue International (1995), 57

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See detailGlageon: une coupe du Givetien en Avesnois (France). Sédimentologie, Coraux, géologie régionale, diagenèse
Boulvain, Frédéric ULg; Coen-Aubert, M.; Mansy, J. L. et al

in Bulletin de la Société Belge de Géologie (1995), 103(1-2), 171-203

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See detailP300 in posttraumatic stress disorder.
Charles, G.; Hansenne, Michel ULg; Ansseau, Marc ULg et al

in Neuropsychobiology (1995), 32(2), 72-4

In the present study, P300 has been recorded in 26 subjects (15 women) 1 month after an aggression without organic complications. Among our sample, 16 subjects fulfilled DSM-III-R criteria for ... [more ▼]

In the present study, P300 has been recorded in 26 subjects (15 women) 1 month after an aggression without organic complications. Among our sample, 16 subjects fulfilled DSM-III-R criteria for posttraumatic stress disorder (PTSD) and 10 did not. P300 amplitude was significantly lower in the 16 PTSD subjects as compared to the 10 subjects without PTSD. This study supports information processing disturbances in PTSD. [less ▲]

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See detailInfluence de la densité osseuse généralisée en implantologie orale
PTAK, Jean ULg; Legrand, Roman; Albert, Adelin ULg et al

in Actualités Odonto-Stomatologiques (1995), 189

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See detailRetinopathy, but not neuropathy, is influenced by the level of residual endogenous insulin secretion in type 2 diabetes.
Bozet, Marie-Claire ULg; Scheen, André ULg; Gerard, Pascale ULg et al

in Diabète & Métabolisme (1995), 21(5), 353-9

The files of 132 patients with Type 2 diabetes were retrospectively studied to characterize the influence of metabolic control and residual insulin secretion on neuropathy and retinopathy, the two most ... [more ▼]

The files of 132 patients with Type 2 diabetes were retrospectively studied to characterize the influence of metabolic control and residual insulin secretion on neuropathy and retinopathy, the two most frequent degenerative diabetic complications. Patients were classified according to their metabolic control (mean HbA1C either < or > or = 8%; reference values: 3-6%) and residual endogenous insulin secretion (fasting plasma C-peptide levels either < or > or = 0.600 nmol/l). Neuropathy was more frequent in patients with poor metabolic control (32/64 = 50%) than in those adequately controlled (17/68 = 25%; p < 0.005). In both subgroups, the level of endogenous insulin secretion did not influence the prevalence of neuropathy. Retinopathy was less effected than neuropathy by the degree of metabolic control (37.5% in the subgroup with HbA1C > or = 8% v.s. 25% in the subgroup with HbA1C < 8%; p < 0.10), but was influenced by residual insulin secretion. Indeed, in patients with inadequate metabolic control, the prevalence of retinopathy was significantly increased in those with higher endogenous insulin secretion (51.4 versus 20.6%, p < 0.02) and thus probably higher insulin resistance. Furthermore, higher systolic arterial blood pressure was observed in the subgroups with a higher prevalence of retinopathy. Such conclusions were confirmed using multivariate analysis. Thus, in Type 2 diabetes, neuropathy is essentially affected by the degree of metabolic control, whereas retinopathy is also influenced by the level of residual endogenous insulin secretion and the presence of systolic hypertension. [less ▲]

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See detailPneumothorax during laparoscopic fundoplication: diagnosis and treatment with positive end-expiratory pressure.
Joris, Jean ULg; Chiche, Jean-Daniel; Lamy, Maurice ULg

in Anesthesia and Analgesia (1995), 81(5), 993-1000

Pneumothorax can develop during laparoscopy, particularly during laparoscopic fundoplication, since the left parietal pleura is exposed and can be torn during dissection in the diaphragmatic hiatus. Such ... [more ▼]

Pneumothorax can develop during laparoscopy, particularly during laparoscopic fundoplication, since the left parietal pleura is exposed and can be torn during dissection in the diaphragmatic hiatus. Such an event will result in specific pathophysiologic changes, since CO2, under pressure in the abdominal cavity, will pass into the pleural space. The aim of this study was to document the pathophysiologic changes induced by pneumothorax, and to evaluate the benefit of positive end-expiratory pressure (PEEP) to treat pneumothorax. Forty-six ASA physical status I and II patients scheduled for laparoscopic fundoplication were monitored extensively; heart rate, mean arterial pressure, end-tidal CO2 (PETCO2), oxygen saturation of hemoglobin (Spo2), minute ventilation, tidal volume, dynamic total lung thorax compliance, and airway pressures were recorded. In 25 patients, oxygen uptake, CO2 elimination and arterial blood gases were also measured. Pneumothorax was diagnosed in seven patients. It resulted in the following pathophysiologic changes: decrease in total lung thorax compliance, increase in airway pressures, and increase in CO2 absorption. Consequently, PACO2 and PETCO2 also increased. Spo2, however, remained normal. The use of PEEP largely corrected these respiratory changes. None of these pneumothoraces required drainage. These data suggest that pneumothorax is common during laparoscopic fundoplication. Early diagnosis is possible by simultaneous monitoring of PETCO2, total lung thorax compliance, and airway pressures. Finally, treatment with PEEP provides an alternative to chest tube placement when pneumothorax is secondary to passage of peritoneal CO2 into the interpleural space. [less ▲]

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See detailPlasma Hormones and Metabolites in Cattle in Relation to Breed (Belgian Blue Vs Holstein) and Conformation (Double-Muscled Vs Dual-Purpose Type)
Clinquart, Antoine ULg; Van Eenaeme, Christian ULg; Mayombo, A. P. et al

in Veterinary Research Communications (1995), 19(3), 185-94

Four Belgian Blue double-muscled type (BBDM) bulls, four Belgian Blue dual-purpose type (BBDP) bulls and four Holstein bulls were used in a fattening trial in order to relate differences in the extent of ... [more ▼]

Four Belgian Blue double-muscled type (BBDM) bulls, four Belgian Blue dual-purpose type (BBDP) bulls and four Holstein bulls were used in a fattening trial in order to relate differences in the extent of muscle development and adiposity to differences in digestibility, endocrine status, protein and lipid metabolism. The larger muscle development in BBDM animals was associated with a trend to higher nitrogen retention, higher food conversion efficiency (p < 0.05) and lower apparent digestibility (p < 0.05). No difference was found between the groups for plasma glucose concentration. Higher creatinine, lower alpha-amino nitrogen, lower triglycerides and higher non-esterified fatty acid plasma levels were observed in BBDM as compared to Holstein bulls (p < 0.05), the BBDP group being intermediate. A trend to a higher cholesterol plasma level was found in BBDM animals. There was no difference between the three groups in plasma fatty acid composition, except for the C14:0 content. Some of the differences in plasma metabolites were related to carcass composition and endocrine regulation, a decrease in muscle development and an increase in adiposity being associated with lower growth hormone production (p < 0.05) and higher insulin (p < 0.05) and IGF secretions. The insulin/growth hormone ratio at the end of the fattening period was 0.0011, 0.0018 and 0.0069 in BBDM, BBDP and Holstein bulls, respectively, and was directly associated with fat deposition. [less ▲]

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See detailEtiologie et physiopathologie du diabète de type 2.
SCHEEN, André ULg; PAQUOT, Nicolas ULg; Lefevre, P.

in Problèmes Actuels d'Endocrinologie et de Nutrition (1995), 10

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See detailNucleotide sequence of the bovine P53 tumor-suppressor cDNA.
Dequiedt, Franck ULg; Willems, Luc ULg; Burny, A. et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1995), 5(4),

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See detailAssociation Of Synthetic Peptide-Fragments Of Human Apolipoprotein-A-I With Phospholipids
Vanloo, B.; Demoor, L.; Boutillon, C. et al

in Journal of Lipid Research (1995), 36(8), 1686-96

The sequences of the plasma apolipoproteins have a high degree of internal homology as they contain several 22-mer internal repeats. These amphipathic helical repeats are considered as the structural and ... [more ▼]

The sequences of the plasma apolipoproteins have a high degree of internal homology as they contain several 22-mer internal repeats. These amphipathic helical repeats are considered as the structural and functional units of this class of proteins. We proposed that the 22-mer repeats of the plasma apolipoproteins consist of 17-mer helical segments separated by extended beta-strands comprising five amino acid residues with a proline in the center of this segment. These beta-strand segments help reverse the orientation of the consecutive helices of apoA-I, A-IV, and E in a discoidal apolipoprotein-phospholipid complex. In order to support this hypothesis, we synthesized apoA-I fragments consisting of, respectively, one putative helix (residues 166-183), one helix plus a beta-strand (residues 161-183), and a pair of helices separated by a beta-strand (residues 145-183). The structural and lipid-binding properties of these peptides were investigated by turbidity, fluorescence, binding studies with unilamellar phospholipid vesicles, electron microscopy, and circular dichroism measurements. Our data show that one single putative helical segment or one helical segment plus one extended beta-strand do not form stable complexes with phospholipids. The addition of a second adjacent helix has no influence on the lipid affinity of the apoA-I 145-183 peptide compared to the shorter segments but substantially improves the stability of the complexes. The helical content of the peptide increases upon lipid association as observed with apoA-I. The complexes generated with the apoA-I 145-183 peptide appear as discoidal particles by negative staining electron microscopy, with heterogeneous sizes ranging between 250 and 450 A. The relative orientation of the peptide and the phospholipid is the same as in a DMPC/apoA-I complex as the helices are oriented parallel to the acyl chains of the phospholipid. However, the stability of these complexes is significantly lower than that of the corresponding DMPC/apoA-I complexes. The transition temperature, fluidity, and cooperativity of the phospholipid bilayer are only weakly affected by the association with the apoA-I 145-183 peptide. These data suggest that a pair of helical peptides linked through a beta-strand associates more tightly with lipids and can form discoidal lipid-peptide complexes, than a single helix. A comparison with the properties of native apoA-I suggests, however, that the cooperativity between pairs of helices in native apoA-I further contributes to strengthen the lipid-protein association. [less ▲]

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See detailTreatment of patent ductus arteriosus by placement of intravascular coils in a pup
Snaps, Frédéric ULg; Mc Entee, Kathleen ULg; Saunders, J. et al

in Journal of the American Veterinary Medical Association [=JAVMA] (1995), 6(6), 724-725

A 3-month-old female mixed-breed pup was referred for evaluation and treatment of patent ductus arteriosus (PDA). Selective angiography was performed to confirm the diagnosis. A cone-shaped PDA with a ... [more ▼]

A 3-month-old female mixed-breed pup was referred for evaluation and treatment of patent ductus arteriosus (PDA). Selective angiography was performed to confirm the diagnosis. A cone-shaped PDA with a large aortic base was outlined. A second selective angiographic procedure was performed to functionally close the PDA by use of thrombotic coils; complete obstruction was obtained. Twenty-four hours after the procedure, the murmur disappeared. One month later, the dog was reexamined and found to be clinically normal. [less ▲]

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See detailLe rôle du plissement disharmonique dans la tectogenèse de l'allochtone ardennais
Mansy, J. L.; Meilliez, F.; Mercier, E. et al

in Bulletin de la Société Géologique de France (1995), 3

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See detailHIV-1 and NF-kappaB activation by photo-oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie ULg; Piret, Bernard

in Photochemistry & Photobiology (1995)

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See detailAbsence of tiGh effect on adaptability to brackish water in Tilapia (Oreochromis niloticus)
Auperin, B.; Leguen, I.; Rentier-Delrue, Françoise ULg et al

in General and Comparative Endocrinology (1995)

The aim of this study was to investigate the possible role of growth hormone in the adaptation of tilapia (Oreochromis niloticus) to brackish water and to analyze its interactions with prolactin in this ... [more ▼]

The aim of this study was to investigate the possible role of growth hormone in the adaptation of tilapia (Oreochromis niloticus) to brackish water and to analyze its interactions with prolactin in this process. Plasma levels of growth hormone do not change upon transfer to brackish water. Treatment of intact tilapia in fresh water with growth hormone prior to transfer did not enable the fish to preadapt to brackish water: the duration of the hydromineral imbalance after transfer was the same in treated animals and controls. The major osmoregulatory role of prolactin in fresh water led us to test the hypothesis that prolactin might antagonize the effect of growth hormone on adaptation to brackish water. Growth-hormone-treated hypophysectomized animals, however, exhibited no increased osmoregulatory capacity as compared to hypophysectomized controls, confirming the absence of a growth-hormone-related osmoregulatory effect. When prolactin and growth hormone were coinjected, growth hormone also proved unable to oppose the Na+ retaining effect of prolactin, in both brackish and fresh water. Surprisingly, hypophysectomized animals adapt better to brackish water than do sham-operated animals. This result is discussed in light of the effects of prolactin and cortisol on osmoregulation in brackish water and we suggest that an important event which allows O. niloticus to adapt to hyperosmotic environment is the reduction of plasma PRL upon transfer to brackish water. [less ▲]

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See detailPulmonary Ventilation, Mechanics, Gas Exchange and Haemodynamics in Calves Following Intratracheal Inoculation of Pasteurella Haemolytica
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in Zentralblatt für Veterinarmedizin. Reihe A (1995), 42(8), 531-544

A Pasteurella haemolytica A1 broth was injected intratracheally in eight calves and measurements of pulmonary function values (PFV) were made once before and hourly post inoculation (p.i.). Changes in ... [more ▼]

A Pasteurella haemolytica A1 broth was injected intratracheally in eight calves and measurements of pulmonary function values (PFV) were made once before and hourly post inoculation (p.i.). Changes in PFVs, included increased respiratory rate and minute ventilation (up to 158% of baseline 2 h p.i.) and decreased tidal volume and lung dynamic compliance (up to 33% of baseline 3 h p.i.). Total pulmonary resistance was not affected. At and after 3 h p.i. there was a progressive impairement of gas exchange, as judged from arterial O2 tension which decreased up to 65% of baseline. In contrast, arterial CO2 tension was not affected. Pulmonary hypertension was observed during the 3 last h of the study and was attributable to an increased pulmonary vascular resistance. Severe neutropenia was observed at 3 h p.i. and post-mortem histological findings were consistent with an acute fibrinohemorragic bronchopneumonia. In conclusion, P. haemolytica airway challenge unequiovocally resulted in acute pneumonia, providing a reproducible pathophysiological model for investigations regarding new therapeutic strategies. [less ▲]

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See detailVieillissement, défenses immunitaires at axe somatotrope-facteurs de croissance apparentés à l'insuline
Kaiser, Marie-Joëlle ULg; Achour, Imane; Kecha, Ouafae et al

in Médecine et Hygiène (1995), 53

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See detailDe réels progrès ont-ils été faits dans le traitement de l'ostéoporose ?
Reginster, Jean-Yves ULg; DEROISY, Rita ULg; Franchimont, P

in Medi-Sphere (1995), 39

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See detailPlace de l'érythropoiétine recombinante en période périopératoire
Janssens, Marc ULg; Lamy, Maurice ULg

in Annales Françaises d'Anesthésie et de Réanimation (1995), 14(Suppl 1), 98-106

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See detailVictor Hugo, "Comme dans les étangs..." : Correction d'un exercice d'analyse textuelle
Tilkin, Françoise ULg; Mathonet, Anne

in Espace pour un humanisme européen (1995), 27

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See detailIron chelation decreases NF-kappaB and HIV-1 activation due to oxidative stress
Sappey, Christine; Boelaert, J. R.; Legrand-Poels, Sylvie ULg et al

in AIDS Research and Human Retroviruses (1995)

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See detailDe la necessite des services d'urgence de la main.
CARLIER, Alain ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1995), 150(7-9), 268-80280-2

Microsurgical techniques have been able to realize anastomoses of small vessels and nerves, with a high rate of success when performed by teams involved in that field. This has changed the concept of ... [more ▼]

Microsurgical techniques have been able to realize anastomoses of small vessels and nerves, with a high rate of success when performed by teams involved in that field. This has changed the concept of surgery of the hand. Reconstruction aims now at a functional result. Organisation of emergencies in hand surgery requires facilitations: hand surgeons, equipment and a team on a 24-hour basis, able to operate any kind of hand trauma. Since 1979, Liege is affiliated to the European Federation. Advantages of the immediate repair are significant repair are significant: reduction of the permanent disability rate, and in many instances, return to work at the same position. In conclusion, early treatment of the injuries of the hand is very effective, but need to be performed by specialists in the field of hand surgery. [less ▲]

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See detailBORN EFFECTIVE CHARGES OF BARIUM-TITANATE - BAND-BY-BAND DECOMPOSITION AND SENSITIVITY TO STRUCTURAL FEATURES
Ghosez, Philippe ULg; GONZE, X.; LAMBIN, P. et al

in Physical Review. B : Condensed Matter (1995), 51(10), 6765-6768

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See detailRobert Feulgen Prize Lecture 1995. New approaches to in situ detection of nucleic acids.
Thiry, Marc ULg

in Histochemistry & Cell Biology (1995), 104(2), 81-95

The present paper reviews recent results obtained by different molecular biology-based, immunocytological approaches to the localization and identification of nucleic acids in sections of biological ... [more ▼]

The present paper reviews recent results obtained by different molecular biology-based, immunocytological approaches to the localization and identification of nucleic acids in sections of biological material. Examples of sensitive, high-resolution detection methods for RNA, DNA or specialized DNA regions are presented. Special emphasis is placed on the potential values and limitations of these new methods. [less ▲]

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See detailEfficacité du piège à fosse pour la collecte d' Hypnoidus riparius (Fabricius) (Coleoptera, Elateridae)
Jeuniaux, C.; Dufrêne, Marc ULg

in Bulletin et Annales de la Société Royale Belge d’Entomologie = Bulletin en Annalen van de Koninklijke Belgische Vereniging voor Entomologie (1995), 131

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See detailModulation of acetylcholine, capsaicin and substance P effects by histamine H3 receptors in isolated perfused rabbit lungs.
Delaunois, Annie ULg; Gustin, Pascal ULg; Garbarg, M. et al

in European Journal of Pharmacology (1995), 277(2-3), 243-50

The modulatory role of histamine H3 receptors in pulmonary oedema induced by acetylcholine, capsaicin and by exogenous substance P was investigated in isolated, ventilated rabbit lungs. Endothelial ... [more ▼]

The modulatory role of histamine H3 receptors in pulmonary oedema induced by acetylcholine, capsaicin and by exogenous substance P was investigated in isolated, ventilated rabbit lungs. Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). Acetylcholine (10(-8) to 10(-4) M), substance P (10(-10) to 10(-6) M), capsaicin (10(-4) M) and 5-hydroxytryptamine (5-HT) (10(-4) M) induced an increase in the Kf,c. Carboperamide, a novel histamine H3 receptor antagonist, induced a significant leftward shift of the concentration-response curve to acetylcholine and also enhanced the effect of capsaicin on the Kf,c, while it had no significant effect on the response to substance P and 5-HT. Imetit, a new histamine H3 receptor agonist, strongly inhibited the effects of acetylcholine and capsaicin. Imetit also strongly protected the lung against substance P effects but did not prevent the 5-HT-induced increase in the Kf,c. Carboperamide completely blocked the inhibitory effect of Imetit on the acetylcholine response. (R)-alpha-Methylhistamine, an other histamine H3 receptor agonist, had the same protective effect against acetylcholine response as Imetit. We conclude that histamine H3 receptors could protect the lung against acetylcholine- and capsaicin-induced oedema via a prejunctional modulatory effect on the C-fibres. However, since the response to exogenous substance P was also inhibited by histamine H3 receptor stimulation, the presence of such receptors at a postsynaptic level, probably on mast cells, was also suggested. [less ▲]

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See detail3,7-Dimethyl-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide zwitterion
Dupont, L.; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Acta Crystallographica (1995), C51

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See detailVaricella-zoster virus induces apoptosis in cell culture
Sadzot-Delvaux, Catherine ULg; Thonard, P.; Schoonbroodt, Sonia et al

in Journal of General Virology (The) (1995), 76(Pt 11), 2875-2879

Apoptosis is an active mechanism of cell death which can be initiated in response to various stimuli including virus infections. In this work, we demonstrate that lytic infection by varicella-zoster virus ... [more ▼]

Apoptosis is an active mechanism of cell death which can be initiated in response to various stimuli including virus infections. In this work, we demonstrate that lytic infection by varicella-zoster virus (VZV), a human herpesvirus, is characterized by nuclear fragmentation of DNA into oligonucleosomal fragments and by chromatin condensation. In vitro, VZV-induced cell death is actually mediated by apoptosis. The mechanisms developed by cells to protect themselves against apoptosis could be one of the parameters allowing the establishment of virus latency. In the case of VZV, which can remain latent in sensory ganglia, we have not yet identified a cellular or viral protein which could play this protective role, since the observed apoptosis mechanism seems to be independent from Bcl-2, the most frequently described inhibitor of apoptosis. [less ▲]

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See detailRecherche expérimentale sur le comportement au feu des colonnes en béton armé
Dotreppe, Jean-Claude ULg; Franssen, Jean-Marc ULg; Baus, Raymond et al

in Annales de l'Institut Technique du Bâtiment et des Travaux Publics [=AIBTP] (1995), 539(254), 25-41

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See detailSecond-generation octarellins: two new de novo (beta/alpha)8 polypeptides designed for investigating the influence of beta-residue packing on the alpha/beta-barrel structure stability
Houbrechts, Annick ULg; Moreau, Benoît; Abagyan, Ruben et al

in Protein Engineering (1995), 8(3), 249-59

The sequence of octarellin I, the first de novo (beta/alpha)8 polypeptide, was revised according to several criteria, among others the symmetry of the sequence, beta-residue volume and hydrophobicity, and ... [more ▼]

The sequence of octarellin I, the first de novo (beta/alpha)8 polypeptide, was revised according to several criteria, among others the symmetry of the sequence, beta-residue volume and hydrophobicity, and charge distribution. These considerations and the overall conclusions drawn from the first design led to two new sequences, corresponding to octarellins II and III. Octarellin II retains perfect 8-fold symmetry. Octarellin III has the same sequence as octarellin II, except for the beta-strands which exhibit a 4-fold symmetry. The two proteins were produced in Escherichia coli. Infrared and CD spectral analyses of octarellins II and III reveal a high secondary structure content. Non-denaturing gel electrophoresis, molecular sieve chromatography and analytical ultracentrifugation suggest that both of these second-generation artificial polypeptides exist as a mixture of a monomer and a dimer form. Octarellins II and III are at least 10 times more soluble than octarellin I. Urea-induced unfolding followed by fluorescence emission suggests that the tryptophan residues, designed to be buried in the (beta/alpha)8, are indeed packed in the hydrophobic core of both proteins. However, octarellin III displays a higher stability towards urea denaturation, indicating that introducing 4-fold symmetry into the beta-barrel might be important for stability of the overall folding. [less ▲]

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See detailLes aliments industriels chez le chien: caractéristiques et utilisation dans différents états physiologiques
Diez, Marianne ULg; Istasse, Louis ULg

in Annales de Médecine Vétérinaire (1995), 139(5), 309-316

Commercial dog food is more and more frequently used as sole diet. The present review characterizes the different types of commercial diets. Principles of bioenergetic allow the calculation of energy ... [more ▼]

Commercial dog food is more and more frequently used as sole diet. The present review characterizes the different types of commercial diets. Principles of bioenergetic allow the calculation of energy density using a prediction equation. Energy requirements are also calculated. Practical use of industrial dog food are illustrated for growing pups, dogs at maintenance or in reproduction. [less ▲]

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See detailGnawing induced by dopaminergic mobilzation : differential effects of direct and indirect dopamine agonists in mice
Tirelli, Ezio ULg; Witkin, J. M.

in Journal of Pharmacology and Experimental Therapeutics (The) (1995), 273

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See detailRegulation of gill prolactin receptors in tilapia (Oreochromis niloticus) after a change in salinity or hypophysectomy
Auperin, B.; Rentier-Delrue, Françoise ULg; Martial, Joseph ULg et al

in Journal of Endocrinology (1995), 145(2), 213-20

Prolactin (PRL) receptors in gill tissue have been analyzed in tilapia (Oreochromis niloticus) after transfer from fresh water (FW) to brackish water (BW). This study has indicated the presence of only ... [more ▼]

Prolactin (PRL) receptors in gill tissue have been analyzed in tilapia (Oreochromis niloticus) after transfer from fresh water (FW) to brackish water (BW). This study has indicated the presence of only one class of tilapia PRL (tiPRL) receptor whatever the salinity. After transfer, however, the percentage of specific binding of the two forms of tiPRL (tiPRLI and tiPRLII) increased significantly. Scatchard analysis of tiPRLI binding indicated an increase in receptor affinity, an effect which was not accompanied by any change in receptor specificity. Transfer to BW also caused the number of tiPRL receptors to increase rapidly, remaining high in fish adapted to BW for 28 days. Based on the sharp reduction in plasma tiPRLI and tiPRLII levels after transfer to BW, one possible explanation may be that tiPRL itself is an important factor regulating the number of free receptors. This hypothesis finds support in the fact that the number of tiPRL receptors also increased in hypophysectomized fish reared in FW. However, the absence of change in receptor affinity after hypophysectomy suggested that yet other factors are involved in tiPRL receptor regulation during the transfer from FW to BW. The paradoxically high numbers of tiPRL receptors in the gills of BW-adapted tilapia, even though PRL is known to be a FW-adapting hormone, is discussed with regard to the environment in which tilapia live. [less ▲]

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See detailFundamentals of transformed skeletal muscle used for cardiac assistance. An overview
Radermacker, M. A.; Chachques, J. C.; Sluse, Francis ULg et al

in Research in Surgery (1995), 7

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See detailModulation of the Expression of Interstitial and Type-Iv Collagenases in Coculture of Ht1080 Fibrosarcoma Cells and Fibroblasts
Munaut, Carine ULg; Noël, Agnès ULg; Weidle, U. H. et al

in Invasion & Metastasis (1995), 15(5-6), 169-78

Members of the metalloproteinase family (MMPs) are known to play a crucial role in the metastatic cascade. Here, we report some investigations about the synthesis of interstitial and type-IV collagenases ... [more ▼]

Members of the metalloproteinase family (MMPs) are known to play a crucial role in the metastatic cascade. Here, we report some investigations about the synthesis of interstitial and type-IV collagenases (gelatinases A and B) in a model of coculture of human fibroblasts and HT 1080 fibrosarcoma cells. The interstitial collagenase activity, mainly found in the conditioned medium of fibroblasts, and its mRNA level were increased in the in vitro coculture model. In contrast, gelatinase A was produced by both cell types. The HT 1080 cells additionally synthesised gelatinase B. In coculture, an enhancement of gelatinase A and the presence of its activated form were observed. Northern blot analysis demonstrated that this enzymatic enhancement occurred at a pretranslational level. The stimulation of the interstitial collagenase activity was partially mediated through soluble factor(s), whereas increased gelatinase A appeared to require direct cell-cell interactions. The extracellular matrix component, type-I collagen, stimulated the enzymatic activities released by the individual cells, but it did not modulate the synthesis of interstitial collagenase in coculture. Our results demonstrate that distinct MMPs are modulated by distinct mechanisms, all depending on specific interactions between tumour cells and host fibroblasts. [less ▲]

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See detailL'étude de la langue russe à l'Université de Liège de 1899 à nos jours: historique, problématique et perspectives
Mouton, Charles ULg

in Actes du Colloque du Sart Tilman des 14 et 15 octobre 1994 (1995)

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See detailMémoire et imagerie mentale
Van der Linden, Martial ULg; Coyette, Françoise; Meulemans, Thierry ULg

in Questions de Logopédie (1995), 31

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See detailKarel Tchapek : humaniste et visionnaire
Mouton, Charles ULg

in Revue Générale (1995), (6/7), 71-80

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See detailA microscopic study of barium titanate
Ghosez, Philippe ULg; Gonze, X.; Michenaud, J. P.

in Ferroelectrics (1995), 164

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See detailComparative double-blind multicenter study of the efficacy of tiludronate and etidronate in Paget's disease of bone
Roux, C; Gennari, C; Farrerons, J et al

in BONE (1995), 16(S1), 503

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See detailEvolution récente du statut de la loutre dans le Parc National des Cévennes
Fonderflick, Jocelyn; De Kermabon, Jean; Libois, Roland ULg et al

in Cahiers d'Ethologie (1995), 15(2-3-4), 233-238

In the south east of the Massif central, the Cevennes National Park covers the area along the dividing line between the Atlantic and Mediterranean catchment areas. The climatic conditions of these medium ... [more ▼]

In the south east of the Massif central, the Cevennes National Park covers the area along the dividing line between the Atlantic and Mediterranean catchment areas. The climatic conditions of these medium-sized mountains are very varied, with a high degree of seasonnality affecting the hydrologic regime of the rivers. The otter seems to have disappeared from that region around the 1960's. Some years ago, evidence was found again ona regular basis in some of these rivers. From 1990, the evolution of this otter population has been monitored by systematic observation of their signe in the principal watercourses covering an area of about 3000 km². At the same time, a preliminary study of the diet (spraint analysis) has been undertaken. Otter signs have been first evidenced on the upper reaches of the rivers Tarn and Lot. In subsequent years, signs have been disovered on some tributaries and on a watercourse flowing to the Mediterranean side: the river Luech. In the river Tarn (upper barbel zone), the most frequent prey is the gudgeon, reaching more than 50% of the total number of prey. However, when the biomass intake is considered, the barbel appears to play the major role, accounting for 46 % of the total food intake. The occurrence of the brown trout (11 %) and its relative biomass (18 %) are weak when compared with the composition of the river fish fauna (25 to 43 % of the estimated fish biomass). [less ▲]

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See detailUse of a model to understand prolactin and growth hormone specificities
Goffin, Vincent; Martial, Joseph ULg; Summers, Neena L.

in Protein Engineering (1995), 8(12), 1215-31

A human prolactin (hPRL) model based on a 2.1 A resolution X-ray refinement of porcine growth hormone is reported. Only regions clearly defined in the growth hormone template (the four-helix bundle) or ... [more ▼]

A human prolactin (hPRL) model based on a 2.1 A resolution X-ray refinement of porcine growth hormone is reported. Only regions clearly defined in the growth hormone template (the four-helix bundle) or previously assumed to be involved in hPRL-receptor binding (the bundle and the binding site loop) are included. A description of the model construction is provided and the resulting hPRL structure is used to interpret mutation/activity data for the cross-reactivity of human growth hormone (hGH) with the lactogenic receptor and the binding of human and bovine prolactin to the lactogenic receptor. The recognition of hPRL by its receptor unexpectedly appears to resemble more closely the interaction of hGH with the somatogenic receptor than with the lactogenic receptor. Each hGH binds to two receptor molecules, and an essential second messenger mediated by correct formation of the receptor-receptor interface has been proposed. The absence of receptor cross-reactivity for hPRL is linked to key sequence changes in hPRL which could disrupt hPRL-somatogenic receptor binding at the second site. A number of previous experiments have relied on the assumption that bioactivity is mediated by homologous residues at topologically equivalent positions. According to the model, this does not appear to be strictly true at either binding site. Good correlation at equivalent positions may be restricted to residues that are important for maintaining binding site shape as well as providing complementary stabilizing interactions between the hormone and receptor. Experiments are proposed to test our hypotheses. [less ▲]

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See detailThe Dissociative Ionization of C2H2. The C+, C2+ and CH2+ Dissociation Channels. The Vinylidene Ion as a Transient?
Locht, Robert ULg; Davister, M.

in Chemical Physics (1995), 195

The dissociative electroionization of C2H2 has been investigated in the C+, C2+ and CH2+ dissociation channels. Both the translational energy and the appearance energies have been measured. The kinetic ... [more ▼]

The dissociative electroionization of C2H2 has been investigated in the C+, C2+ and CH2+ dissociation channels. Both the translational energy and the appearance energies have been measured. The kinetic energy versus appearance energy diagrams related to these species are discussed in detail and dissociation mechanisms are proposed for all observed onset energies. For the three ionic species, the dissociation pathways leading to the fragmentation of C2H2+ into C+, C2+ and CH2+ successively have to run very likely over an isomerization mechanism of the molecular ion. The isomeric vinylidene structure could be the most probable intermediate. [less ▲]

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See detailThermographie routière nocturne de la Région Wallonne : méthodologie, présentation des résultats et intérêts de l'approche topoclimatologique
Funcken, Ph.; Erpicum, Michel ULg; Frédéric, Marc ULg et al

in Publications de l'Association Internationale de Climatologie (1995), 8

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See detailThe appearance energy of C2H+ from C2H2 revisited. A Photoion-Photoelectron Coincidence Spectroscopic Determination.
Servais, Christian ULg; Locht, Robert ULg

in Chemical Physics Letters (1995), 236

The dissociative ionization of C2H2 in the C2H+-producing channel has been reconsidered. The photoion-photoelectron coincidence (PIPECO) technique has been used. The C2H+/e coincidence rate has been ... [more ▼]

The dissociative ionization of C2H2 in the C2H+-producing channel has been reconsidered. The photoion-photoelectron coincidence (PIPECO) technique has been used. The C2H+/e coincidence rate has been recorded between 16.2 and 21.2 eV. The lowest appearance energy determined for C2H+ is 17.35+/-0.04 eV. A second appearance energy is measured at 18.40+/-0.04 eV. At both energies ethynyl ions are produced through a predissociation mechanism at the expense of the C2H2+(A2Ag) state at 17.35 eV and C2H2+(B2Sigma+/g) at 18.40 eV. These results are in good agreement with the most recent TPIPECO experiments and dissociative electroionization results. This comparison leads to the exclusion of any contribution of autoionization phenomena to the C2H+ ion production between 16.2 and 21.2 eV. Investigations of C2H+ through the ion-pair formation channel (and dissociative electron attachment producing C2H- and H-) in C2H2 are reported and its contribution to C2H+ ion production below 17.3 eV must be discarded. [less ▲]

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See detailL'hypothyroidie subclinique.
Moreau, L.; Beckers, Albert ULg

in Revue Médicale de Liège (1995), 50(4), 171-4

L'hypothiroïdie subclinique associe, chez des patients asymptomatiques, une élévation de la TSH sérique et une hormonologie thyroïdienne dans les limites de la normale. Le traitement de cette pathologie ... [more ▼]

L'hypothiroïdie subclinique associe, chez des patients asymptomatiques, une élévation de la TSH sérique et une hormonologie thyroïdienne dans les limites de la normale. Le traitement de cette pathologie affectant surtout les personnes âgées est justifié par l'existence de troubles du bilan lipidique, de goitres et de perturbations neuropsychologiques. Celui-ci repose sur l'administration de lévothyroxine à dose minimale pour normaliser la TSH [less ▲]

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See detailVaricella-zoster virus gene regulation
Piette, Jacques ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Neurology (1995), 45(12, suppl. 8), 23-27

The varicella-zoster virus genome contains 71 open reading frames (ORFs), five of which (ORF62, ORF4, ORF63, ORF61, and ORF10) encode regulatory proteins. ORF62 codes for the major immediate early protein ... [more ▼]

The varicella-zoster virus genome contains 71 open reading frames (ORFs), five of which (ORF62, ORF4, ORF63, ORF61, and ORF10) encode regulatory proteins. ORF62 codes for the major immediate early protein of the virus exhibiting DNA-binding and regulatory functions. This protein, localized in the cell nucleus, is a functional homologue to ICP4 of herpes simplex virus type 1 (HSV-1). It trans-activates several varicella-zoster virus promoters of the various gene classes and autoregulates its own expression. ORF4 protein activates gene promoters provided they have basal activities, but it is not a functional homologue of HSV-1 ICP27. Gene regulation activity appears to be linked to its cysteine-rich C-terminal region. ORF63 codes for an immediate early protein mainly located in the cell nucleus. The regulatory functions it performs are still unclear. ORF61 protein is the functional homologue of HSV-1 ICPO. Its N-terminal region exhibits a RING domain responsible for trans-activating and trans-repressing activities. ORF10 protein exhibits similarities with HSV-1 VP16 and activates the ORF62 promoter. [less ▲]

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See detailExtraction d'une canine chez un ours polaire
DUFOUR, Pascale ULg; Balligand, Marc ULg; Pypendop, B. et al

in Annales de Médecine Vétérinaire (1995)

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See detailLight reflection model for lighting applications
Embrechts, Jean-Jacques ULg

in Lighting Research & Technology (1995), 27(4)

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See detail3-Methyl-4H-pyrido[4,3-e][1,2,4]thiadiazine 1,1-dioxide monohydrate
Dupont, L.; De Tullio, Pascal ULg; Pirotte, Bernard ULg et al

in Acta Crystallographica (1995), C51

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See detailFracture de la hanche. Données épidémiologiques et approche thérapeutique
Reginster, Jean-Yves ULg

in Le Monde Médical de la Femme (1995), 8

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See detailNuclear transplantation using bovine primordial germ cells from male fetuses.
Delhaise, F.; Ectors, Fabien ULg; de Roover, R. et al

in Reproduction, Fertility and Development (1995), 7(5), 1217-9

The developmental potential of nuclei of bovine gonial cells was investigated by nuclear transfer. Gonial cells were collected from male fetuses at about 175 days post coitum (p.c.). They were fused with ... [more ▼]

The developmental potential of nuclei of bovine gonial cells was investigated by nuclear transfer. Gonial cells were collected from male fetuses at about 175 days post coitum (p.c.). They were fused with enucleated oocytes; reconstituted embryos were cultured in vitro for 7 days. Embryos reaching the compacted morula or blastocyst stage were either fixed for cell counting or transferred into recipients. Out of 115 oocyte-gonia fusions, 101 (87.8%) gave rise to cleaved embryos at Day 3 and 26 (22.6%) had reached the 8-cell stage. At Day 7, 1 (1%) developed to the morula stage and 5 (4%) reached the blastocyst stage. Three blastocysts were fixed and showed normal cell numbers (135; 90; 76 cells). Three blastocysts and one morula were transferred in four recipients; two recipients were pregnant at Day 21 but only one was positive at Day 35 p.c.; this last one aborted around Day 40 p.c. No conceptus was collected. These results indicate that gonial cell nuclei can be partially reprogrammed; they are able to develop into blastocysts and to initiate gestation. However, more experiments will be necessary to prove the nuclear totipotency of bovine gonial cells. [less ▲]

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See detailClinical neurophysiology and neurotransmitters
MAERTENS DE NOORDHOUT, Alain ULg; Wang, Wei; Schoenen, Jean ULg

in Cephalalgia : An International Journal of Headache (1995), 15(4), 301-9

Clinical neurophysiology allows non-invasive assessment of neurotransmitter function in various regions of the central and peripheral nervous system. In this review, we describe examples of functional ... [more ▼]

Clinical neurophysiology allows non-invasive assessment of neurotransmitter function in various regions of the central and peripheral nervous system. In this review, we describe examples of functional evaluation of neurotransmission at the neuromuscular junction, in some spinal interneurons and intracortical circuits as well as evaluation of pharmacological modulation of some electrophysiological tests. These investigations are carried out to help our understanding of the pathophysiology of brain diseases. Finally, we discuss possible relationships between electrophysiological tests (evoked/event-related potentials and exteroceptive suppression of temporalis muscle activity) and neurotransmitter function in headache. [less ▲]

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See detailGrecs et Latins face aux langues étrangères. Contribution à l'étude de la diversité linguistique dans l'antiquité classique
Rochette, Bruno ULg

in Revue Belge de Philologie et d'Histoire (1995), 73(1), 5-16

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See detailProtective Effect of Various Antagonists of Inflammatory Mediators against Paraoxon-Induced Pulmonary Edema in the Rabbit
Delaunois, Annie ULg; Gustin, Pascal ULg; Vargas, M. et al

in Toxicology and Applied Pharmacology (1995), 132(2), 343-345

The protective effect of some antagonists of various inflammatory mediators against paraoxon-induced increases in endothelial permeability has been investigated in isolated perfused rabbit lungs. The ... [more ▼]

The protective effect of some antagonists of various inflammatory mediators against paraoxon-induced increases in endothelial permeability has been investigated in isolated perfused rabbit lungs. The edema induced by paraoxon has been previously related to a chain reaction mediated by acetylcholine. Lungs were ventilated and blood-free perfused with a constant flow. Arterial and venous pressures and lung weight were continuously recorded. Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). Paraoxon (4 x 10(-4) M) was injected in the perfusion circuit, in lungs with or without pretreatment with atropine, ketanserin, clonidine, morphine, indomethacin, and terfenadine plus cimetidine. Paraoxon induced a time-dependent increase in the Kf,c, a maximal effect being recorded 60 min after the injection. All the antagonists used as pretreatment significantly reduced the maximal effect recorded after paraoxon. These results show that muscarinic receptor antagonists, inhibitors of neuropeptides release, cyclooxygenase inhibitors, and 5-hydroxytryptamine and histamine receptor antagonists can protect the lung against the edema induced by paraoxon. This protective effect is due to inhibition of the chain reaction triggered by acetylcholine. [less ▲]

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See detailThe Cenozoic evolution of the strontium and carbon cycles: Relative importance of continental erosion and mantle exchanges
Godderis, Y.; François, Louis ULg

in Chemical Geology (1995), 126(2), 169-190

The past variations of the seawater Sr-87/Sr-86 isotopic ratio are related to changes in the relative contribution of the mantle Sr input to the ocean and the Sr supply from continental weathering ... [more ▼]

The past variations of the seawater Sr-87/Sr-86 isotopic ratio are related to changes in the relative contribution of the mantle Sr input to the ocean and the Sr supply from continental weathering. Recently, it has been postulated that the Cenozoic increase in the seawater Sr-87/Sr-86 isotopic ratio was associated with the uplift of the Himalayan and Andean mountains at that time. These orogenies may have changed the Sr isotopic ratio of the continental rocks undergoing weathering (as a result of extensive metamorphism), increased the river flux of Sr through enhanced weathering in these regions and possibly caused the global climatic cooling trend of the Cenozoic. A model of the major geochemical cycles coupled to an energy balance climate model is used to explore the possible causes of the Mesozoic-Cenozoic fluctuations in the seawater Sr-87/Sr-86 isotopic ratio. The contribution of the mantle exchanges at mid-ocean ridges, of the recycling of seafloor carbonates through plate margin volcanism and of the alteration of seafloor basalts to the fluctuations of the seawater Sr-87/Sr-86 isotopic ratio are studied. Finally, this model tentatively describes the impact of the Himalayan orogeny on the geochemical cycles of Sr and C. Some possible effects of the extensive metamorphism associated with the India-Asia collision and of the Himalayan uplift are modelled. The model reproduces the Cenozoic increase of the seawater Sr-87/Sr-86 isotopic ratio. However, the impact of the Himalayan orogeny on the C cycle appears to be limited and insufficient to generate the global climatic cooling of the Cenozoic. Rather, in the model, the Cenozoic cooling is mostly due to the reduction of the CO2 emission from mid-ocean ridge volcanism and to changes in the chemical weathering rates in the rest of the world excluding the Himalayas. [less ▲]

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See detailThiamine Homeostasis in Neuroblastoma Cells
Bettendorff, Lucien ULg

in Neurochemistry International (1995), 26(3), 295-302

We recently showed that thiamine uptake by neuroblastoma cells is mediated by two saturable transport system: the first with high affinity for thiamine (Km = 35 nM) is blocked by veratridine; the other ... [more ▼]

We recently showed that thiamine uptake by neuroblastoma cells is mediated by two saturable transport system: the first with high affinity for thiamine (Km = 35 nM) is blocked by veratridine; the other, with low affinity is blocked by Ca2+. The driving force for thiamine uptake is its phosphorylation to thiamine diphosphate (TDP) by thiamine pyrophosphokinase and subsequent binding of this cofactor to apoenzymes. Our results suggest that cells of neuronal origin possess mechanisms regulating the intracellular concentration of thiamine. At low external thiamine, the vitamin is taken up by a high-affinity transporter and pyrophosphorylated in thiamine diphosphate (TDP): this is the TDP pool of slow turnover. An intraover extracellular concentration gradient of free thiamine is observed at low external concentration of the vitamin. At higher external thiamine concentration, TDP accumulation is limited by the binding capacity to the apoenzymes and unbound TDP (i.e. a small pool of fast turnover) is quickly hydrolyzed. Thiamine is slowly released by the cells by at least two different mechanisms. The first, accounting for a maximum of 50% of total thiamine release, is stimulated by external thiamine and is blocked by veratridine, suggesting that it is a self-exchange mechanism catalyzed by the high affinity thiamine transporter. The remaining thiamine efflux is neither sensitive to veratridine nor to Ca2+ and its mechanism is unknown. About 25% of intracellular thiamine is not released, even after treatment of the cells with digitonin, thus maintaining an apparent gradient. This suggests a binding or sequestration in intracellular compartments.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailPacemaker Implantation for Early Sinus Node Dysfunction after Orthotopic Heart Transplantation
RADERMECKER, Marc ULg; Defraigne, Jean-Olivier ULg; Fourny, J. et al

in Acta Chirurgica Belgica (1995), 95(1), 31-4

Among 60 patients who received OHT at our institution between November 1986 and January 1990, 4 actually needed implantation of a permanent pacemaker. Three patients with symptomatic early sinus node ... [more ▼]

Among 60 patients who received OHT at our institution between November 1986 and January 1990, 4 actually needed implantation of a permanent pacemaker. Three patients with symptomatic early sinus node dysfunction were identified and were satisfactorily paced on the VVIR mode. All three showed sinus recovery within three months following implantation and had their pacemaker switched from the VVIR mode to a simple ventricular demand pacing. Interestingly, administration of beta-blocking drugs quickly reinstituted pace dependence. The patients' outcome is excellent (18 to 48 months follow-up). No difference with the global cohort of our OHT recipients was noticed, apart from an apparent high incidence of infections (3/3) with (2/3) CMV and severe rejection (3/3) during the first month postoperatively. This data suggests that sinus dysfunction may be the result of a multifactorial (rejection, CMV, ...) injury to the conduction system, and is only clinically relevant beyond a threshold level. Denervation hypersensitivity, together with correction of these factors, may account for the restoration of a sufficient reserve of conduction system, and therefore appropriate sinus node function in basal conditions. [less ▲]

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See detailThiamine Deficiency--Induced Partial Necrosis and Mitochondrial Uncoupling in Neuroblastoma Cells Are Rapidly Reversed by Addition of Thiamine
Bettendorff, Lucien ULg; Sluse, Francis ULg; Goessens, Guy ULg et al

in Journal of Neurochemistry (1995), 65(5), 2178-2184

Culture of neuroblastoma cells in a medium of low-thiamine concentration (6 nM) and in the presence of the transport inhibitor amprolium leads to the appearance of overt signs of necrosis; i.e., the ... [more ▼]

Culture of neuroblastoma cells in a medium of low-thiamine concentration (6 nM) and in the presence of the transport inhibitor amprolium leads to the appearance of overt signs of necrosis; i.e., the chromatin condenses in dark patches, the oxygen consumption decreases, mitochondria are uncoupled, and their cristae are disorganized. Glutamate formed from glutamine is no longer oxidized and accumulates, suggesting that the thiamine diphosphate-dependent alpha-ketoglutarate dehydrogenase activity is impaired. When thiamine (10 microM) is added to the cells, the O2 consumption increases, respiratory control is restored, and normal cell and mitochondrial morphology is recovered within 1 h. Succinate, which is oxidized via the thiamine diphosphate-independent succinate dehydrogenase, is also able to restore a normal O2 consumption (with respiratory control) in digitonin-permeabilized thiamine-deficient cells. Our results therefore suggest that the slowing of the citric acid cycle is the main cause of the biochemical lesion induced by thiamine deficiency as observed in Wernicke's encephalopathy. [less ▲]

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See detailActivation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro.
Sheridan, P. L.; Sheline, C. T.; Cannon, K. et al

in Genes & Development (1995), 9(17), 2090-104

Lymphoid enhancer-binding factor 1 (LEF-1) is a regulatory high mobility group (HMG) protein that activates the T cell receptor alpha (TCR alpha) enhancer in a context-restricted manner in T cells. In ... [more ▼]

Lymphoid enhancer-binding factor 1 (LEF-1) is a regulatory high mobility group (HMG) protein that activates the T cell receptor alpha (TCR alpha) enhancer in a context-restricted manner in T cells. In this paper we demonstrate that the distal region of the human immunodeficiency virus-1 (HIV-1) enhancer, which contains DNA-binding sites for LEF-1 and Ets-1, also provides a functional context for activation by LEF-1. First, we show that mutations in the LEF-1-binding site inhibit the activity of multimerized copies of the HIV-1 enhancer in Jurkat T cells, and that LEF-1/GAL4 can activate a GAL4-substituted HIV-1 enhancer 80- to 100-fold in vivo. Second, recombinant LEF-1 is shown to activate HIV-1 transcription on chromatin-assembled DNA in vitro. By using a nucleosome-assembly system derived from Drosophila embryos, we find that the packaging of DNA into chromatin in vitro strongly represses HIV-1 transcription and that repression can be counteracted efficiently by preincubation of the DNA with LEF-1 (or LEF-1 and Ets-1) supplemented with fractions containing the promoter-binding protein, Sp1. Addition of TFE-3, which binds to an E-box motif upstream of the LEF-1 and Ets-1 sites, further augments transcription in this system. Individually or collectively, none of the three enhancer-binding proteins (LEF-1, Ets-1, and TFE-3) could activate transcription in the absence of Sp1. A truncation mutant of LEF-1 (HMG-88), which contains the HMG box but lacks the trans-activation domain, did not activate transcription from nucleosomal DNA, indicating that bending of DNA by the HMG domain is not sufficient to activate transcription in vitro. We conclude that transcription activation by LEF-1 in vitro is a chromatin-dependent process that requires a functional trans-activation domain in addition to the HMG domain. [less ▲]

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See detailIsolated Atherosclerotic Aneurysms of the Iliac Arteries
Desiron, Quentin ULg; Detry, Olivier ULg; Sakalihasan, Natzi ULg et al

in Annals of Vascular Surgery (1995), 9(Suppl), 62-6

Atherosclerotic aneurysms limited to the iliac arteries are rare and entail a high risk of rupture. To evaluate the efficacy of prophylactic surgery, we retrospectively studied 15 patients (13 men and two ... [more ▼]

Atherosclerotic aneurysms limited to the iliac arteries are rare and entail a high risk of rupture. To evaluate the efficacy of prophylactic surgery, we retrospectively studied 15 patients (13 men and two women; mean age 69 years) treated for isolated iliac aneurysms in the Department of Cardiovascular Surgery at the University Hospital of Liege over a period of 18 years. They had a total of 25 aneurysms (20 common iliac and five internal iliac). Six patients were treated electively and nine on an emergency basis for rupture. Five of the emergency patients (33%) died in the early postoperative period (< 30 days); in each case the aneurysm had ruptured and an emergency operation was performed (55.5% mortality in the ruptured iliac aneurysm group). On the other hand, all patients treated electively survived. Our study is comparable to other recent series in the literature, which also reported a high incidence of rupture and death in emergency operations. Prophylactic elective surgery is recommended for iliac aneurysms. [less ▲]

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See detailLe cas clinique du mois. Troubles electrolytiques severes dans l'anorexie mentale.
Scheen, André ULg

in Revue Médicale de Liège (1995), 50(1), 16-7

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See detailIS THE PRESENCE OF MESOTROPHIC PLANT-COMMUNITIES IN THE PEAT-BOGS OF HAUTES-FAGNES (BELGIUM) CONNECTED WITH TONALITE INTRUSIONS IN THE REVINIAN LAYERS
Hambuckers, Alain ULg; Schumacker, René ULg; Remacle, Jean ULg et al

in Belgian Journal of Botany (1995), 128(1), 48-56

Within the Hautes-Fagnes Nature Reserve (Belgium) acidic and oligotrophic biotopes are developing on very acidified stony silt raised bogs. However, they are locally dominated by mesotrophic species ... [more ▼]

Within the Hautes-Fagnes Nature Reserve (Belgium) acidic and oligotrophic biotopes are developing on very acidified stony silt raised bogs. However, they are locally dominated by mesotrophic species. Based on the fact that there are several outcrops of tonalite in the region and particularly one in the Nature Reserve, a hypothesis to explain these singularities of the vegetation implies discontinuities of the bedrock provoked by intrusions of this magmatic rock in the Revinian layer covering the region. Seventeen plots containing Phragmites australis were examined and vegetation was described. Silt and soil water were sampled and analyzed for mineral contents in 15 sites. The differences of mineral composition between the P. australis plots and their surrounding environment confirmed the more mesotrophic characteristics of these plots (i.e. higher pH, higher Ca and Mg contents, lower Al content). However, the lower Zn, Pb and Fe contents would lead to reject the hypothesis of intrusions of tonalite since these elements are more abundant in the tonalite and its mineralization than in the Revinian rock. Nevertheless, the overall ecological conditions of the examined plots and the differences of plant species composition suggested that the observed chemical environment could be evoked for explaining the distribution of the mesotrophic species in the oligotrophic environment of the Nature Reserve. Consequently, an alternative hypothesis is proposed. [less ▲]

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See detailEfficacité des solutions envisagées par des enseignants en formation face à des problèmes rencontrés dans des leçons d’éducation physique
Beckers-Ledent, Maryse; Cloes, Marc ULg; Delfosse, Catherine et al

in Revue de l'Education Physique (1995), 35(4), 173-179

La recherche sur l'efficacité de l'enseignement s'est concrétisée et a trouvé application dans plusieurs ouvrages de didactique. Elle a été menée à partir de données directement observables, les ... [more ▼]

La recherche sur l'efficacité de l'enseignement s'est concrétisée et a trouvé application dans plusieurs ouvrages de didactique. Elle a été menée à partir de données directement observables, les comportements et stratégies d'enseignement dans l'action en classe. L'observation des comportements des enseignants, des élèves et des interactions entre ces différents acteurs a contribué à identifier des variables du processus en relation positive ou négative avec les progrès des élèves. ... [less ▲]

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See detailEpithelioid Cell Histiocytoma: A Report of Two Cases
Dezfoulian, Bita ULg; Nikkels, Arjen ULg; Pierard-Franchimont, Claudine ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1995), 190(4), 349-350

Epithelioid cell histiocytoma is a rarely reported tumor derived from factor-XIIIa-positive dermal dendrocytes. Two additional cases are presented including their clinical, histologic and ... [more ▼]

Epithelioid cell histiocytoma is a rarely reported tumor derived from factor-XIIIa-positive dermal dendrocytes. Two additional cases are presented including their clinical, histologic and immunohistochemical features. [less ▲]

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See detailModulation of the Clozapine Structure Increases Its Selectivity for the Dopamine D4 Receptor
Liégeois, Jean-François ULg; Bruhwyler, J.; Damas, Jacques ULg et al

in European Journal of Pharmacology (1995), 273(3), 1-3

Clozapine has a more marked affinity for the recently cloned dopamine D4 receptor than for the dopamine D2 receptor. In the search for a selective ligand for the dopamine D4 receptor, useful as a ... [more ▼]

Clozapine has a more marked affinity for the recently cloned dopamine D4 receptor than for the dopamine D2 receptor. In the search for a selective ligand for the dopamine D4 receptor, useful as a pharmacological tool or as a potent atypical antipsychotic, a pyridobenzodiazepine derivative bioisoster of clozapine, JL 18, 8-methyl-6-(4-methyl-1-piperazinyl)-11H-pyrido [2,3-b][1,4]benzodiazepine, was found to be the most dopamine D4-selective ligand belonging to the diarylazepine class. Indeed, JL 18 binds to the dopamine D4 receptor with affinity up to 25 times superior to that for the dopamine D2 receptor and presents reduced affinities for other receptors. [less ▲]

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See detailAccroissement des exportations agricoles et sécurité alimentaire au Viêt-nam
Lebailly, Philippe ULg; Burny, Philippe ULg

in Economies et sociétés. Série Développement agro-alimentaire, A.G. (1995), 22(3-4), 361-371

La rénovation des politiques et des méthodes d'action à l'égard de l'agriculture a permis au Viêt-nam de conforter sa position de troisième exportateur mondial de riz. Néanmoins la situation semble moins ... [more ▼]

La rénovation des politiques et des méthodes d'action à l'égard de l'agriculture a permis au Viêt-nam de conforter sa position de troisième exportateur mondial de riz. Néanmoins la situation semble moins favorable qu'il n'y paraît. Le marché international du riz est caractérisé par des prix bas et par une forte concurrence entre pays exportateurs. En outre, le marché est réduit et instable. Il s'ensuit que le Viêt-nam, proche de l'autosuffisance, peut tantôt être importateur tantôt exportateur de riz selon les performances enregistrées par les riziculteurs. Cet article analyse les perspectives qui sont actuellement offertes au Viêt-nam en matière de sécurité alimentaire en relation avec l'adoption d'une politique agricole qui encourage l'accroissement des exportations de riz. [less ▲]

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See detailLe risque familial de l'anévrisme de l'aorte abdominale et ses conséquences pour l'organisation d'un dépistage sélectif
Limet, Raymond ULg

in Journal des Maladies Vasculaires (1995), 20(4), 285-7

Aneurysm of the abdominal aorta (AAA) is multifactorial disease related to acquired factors (arteriosclerosis) and genetic factors (collagen anomalies) with, in some cases, a familial component. The ... [more ▼]

Aneurysm of the abdominal aorta (AAA) is multifactorial disease related to acquired factors (arteriosclerosis) and genetic factors (collagen anomalies) with, in some cases, a familial component. The incidence of familial AAA was evaluated in 324 patients in 313 families who underwent surgery for AAA. A family survey was used to determine whether members of the operated patients had AAA or had died due to ruptured aneurysms. There were 276 cases (including 12 females) with sporadic AAA and 81 cases (including 5 females) who were members of families with at least one other member having AAA. Comparison between sporadic AAA (SAAA) and familial AAA (FAAA) demonstrated the following differences: 1) diagnosis was made earlier in FAAA (p < 0.05) than in SAAA (64.1 versus 66 years of age respectively), 2) patients with FAAA were younger at the time of rupture (65.4 versus 75.1 years, p < 0.001), 3) the incidence of rupture was greater in FAAA than in SAAA (32.4% versus 8.7% p < 0.001), and finally 4) the relative risk of developing AAA was 18 times greater in members of a family with AAA than in the reference population. Early screening with ultrasonic examinations in at least patient's brother's over 50 years would not be an unjustified expense. The frequency of later examinations would depend on the size of the aorta initially observed. If FAAA is evidenced, under normal conditions, corrective surgery should be proposed earlier than for sporadic AAA. [less ▲]

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