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See detailRed blood cell precursor mass as an independent determinant of serum erythropoietin level.
Cazzola, M.; Guarnone, R.; Cerani, P. et al

in Blood (1998), 91(6), 2139-45

Serum erythropoietin (sEpo) concentration is primarily related to the rate of renal production and, under the stimulus of hypoxia, increases exponentially as hemoglobin (Hb) decreases. Additional factors ... [more ▼]

Serum erythropoietin (sEpo) concentration is primarily related to the rate of renal production and, under the stimulus of hypoxia, increases exponentially as hemoglobin (Hb) decreases. Additional factors, however, appear to influence sEpo, and in this work, we performed studies to evaluate the role of the red blood cell precursor mass. We first compared the relationship of sEpo with Hb in patients with low versus high erythroid activity. The first group included 27 patients with erythroid aplasia or hypoplasia having serum transferrin receptor (sTfR) levels < 3 mg/L (erythroid activity < 0.6 times normal), while the second one included 28 patients with beta-thalassemia intermedia having sTfR levels > 10 mg/L (erythroid activity > 2 times normal). There was no difference between the two groups with respect to Hb (8.3 +/- 1.6 v 8.0 +/- 1.3 g/dL, P > .05), but sEpo levels were notably higher in patients with low erythroid activity (1,601 +/- 1,542 v 235 +/- 143 mU/mL, P < . 001). In fact, multivariate analysis of variance (ANOVA) showed that, at any given Hb level, sEpo was higher in patients with low erythroid activity (P < .0001). Twenty patients undergoing allogeneic or autologous bone marrow transplantation (BMT) were then investigated. A marked increase in sEpo was seen in all cases at the time of marrow aplasia, disproportionately high when compared with the small decrease in Hb level. Sequential studies were also performed in five patients with iron deficiency anemia undergoing intravenous (IV) iron therapy. Within 24 to 72 hours after starting iron treatment, marked decreases in sEpo (up to one log magnitude) were found before any change in Hb level. Similar observations were made in patients with megaloblastic anemia and in a case of pure red blood cell aplasia. These findings point to an inverse relationship between red blood cell precursor mass and sEpo: at any given Hb level, the higher the number of red blood cell precursors, the lower the sEpo concentration. The most likely explanation for this is that sEpo levels are regulated not only by the rate of renal production, but also by the rate of utilization by erythroid cells. [less ▲]

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See detailEtude contrastive de la notion temps/aspect dans les langues indo-européennes
Mouton, Charles ULg

in Slavica Gandensia (1998), (25/1), 29-41

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See detailA bottom-up approach towards marker assisted selection
Mackinnon, M.; Georges, Michel ULg

in Livestock Production Science (1998), 54

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See detailIn Vitro And In Vivo Oncogenic Potential Of Bovine Leukemia Virus G4 Protein
Kerkhofs, P.; Heremans, H.; Burny, A. et al

in Journal of Virology (1998), 72(3),

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See detailLe cartilage articulaire peut-il se régénérer ? (2ème partie)
Henrotin, Yves ULg; Reginster, Jean-Yves ULg

in Le Généraliste (1998), 415

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See detailHet hoogst haalbare
Spinoy, Erik ULg

in Nieuw Wereld Tijdschrift (1998), 5(4), 74-75

This is a review of Dutch translations of Hölderlin's poetry, made by the Dutch writer and critic Ad den Besten.

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See detail3 ',4 ',5 ',6 ' tetradehydrolongicaudatine Y, an anhydronium base from Strychnos usambarensis
Frederich, Michel ULg; Quetin-Leclercq, J.; Biala, R. G. et al

in Phytochemistry (1998), 48(7), 1263-1266

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See detailEstimation de la précision de la superposition de polygones à travers la propagation des erreurs
Semlali, El Hassan; Donnay, Jean-Paul ULg

in Bulletin de la Société Française de Photogrammétrie et Télédétection (1998), 1998(2), 28-33

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See detailImpairment of the mitochondrial electron chain transport prevents NF-kappa B activation by hydrogen peroxide.
Josse, C.; Legrand-Poels, S.; Piret, B. et al

in Free Radical Biology & Medicine (1998), 25(1), 104-112

A large body of work has been devoted to mechanisms leading to the activation of the transcription factor NF-kappa B in various cell types. Several studies have indicated that NF-kappa B activation by ... [more ▼]

A large body of work has been devoted to mechanisms leading to the activation of the transcription factor NF-kappa B in various cell types. Several studies have indicated that NF-kappa B activation by numerous stimuli depends on the intracellular generation of reactive oxygen species (ROS). In this report, we first demonstrated that inhibition of the electron transport chain by either rotenone or antimycine A gave rise to dose-dependent inhibition of NF-kappa B translocation induced by 150 microM of hydrogen peroxide (H2O2). Conversely, the impairment of the mitochondrial respiratory chain did not affect T lymphocyte treatment by TNF-alpha (tumor necrosis factor alpha) or pre-B lymphocyte treatment with LPS (lipopolysaccharide). We also showed that oligomycine which inhibits ATP synthase and FCCP, which uncouples respiration also led to dose-dependent inhibition of NF-kappa B activation by H2O2. All these inhibitors were also shown to inhibit mitochondrial respiration in lymphocytes assessed by oxygen consumption. Although only a transient drop in ATP concentration was observed when lymphocytes were treated by H2O2, this effect was remarkably reinforced in the presence of oligomycine demonstrating the crucial role of ATP in the signal transduction pathway induced by H2O2. [less ▲]

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See detailThe phosphoprotein pp135 is an essential constituent of the fibrillar components of nucleoli and of coiled bodies.
Vandelaer, M.; Thiry, Marc ULg

in Histochemistry & Cell Biology (1998), 110(2), 169-77

We examined the distribution of the silver-stainable phosphoprotein, pp135, within Ehrlich tumor and HEp-2 cells by a postembedding Lowicryl immunogold labeling procedure. Identical labeling patterns were ... [more ▼]

We examined the distribution of the silver-stainable phosphoprotein, pp135, within Ehrlich tumor and HEp-2 cells by a postembedding Lowicryl immunogold labeling procedure. Identical labeling patterns were obtained in both cell types. During interphase, gold particles were found not only over the dense fibrillar component but were also evident over the fibrillar centers of nucleoli. By contrast, the granular component did not display any significant label. When rRNA synthesis was inhibited by actinomycin D, the same labeling was observed in segregated nucleoli; both fibrillar components were labeled. Aside from the nucleolar labeling, label was also consistently present in coiled bodies. During metaphase, label was visualized in silver-stainable material of the nucleolus organizing regions. It thus appears that, unlike the two major silver-stained proteins, nucleolin/C23 and B23, pp135 remains located in all major silver-stainable structures during the whole cell cycle. This finding strongly suggests that pp135 could be the component responsible for in situ silver staining. On the other hand, the maintenance of pp135 in the fibrillar centers throughout the cell cycle, like RNA polymerase I, upstream binding factor, and DNA topoisomerase I, suggests that pp135 could be a component involved in transcription of the rRNA genes. [less ▲]

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See detailObservation de la peau d'un hadrosaure vieux de 75 millions d'années.
Pierard, Sébastien ULg; Franchimont, Claudine ULg; Pierard, Gérald ULg

in Revue Verviétoise d'Histoire Naturelle (1998), 55

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See detailLiver assist systems: state of the art.
Arkadopoulos, N.; Detry, Olivier ULg; Rozga, J. et al

in International Journal of Artificial Organs (1998), 21(12), 781-7

Attempts to develop liver support systems for the treatment of patients with liver failure have ranged from use of plasma exchange to utilization of charcoal columns and extracorporeal devices loaded with ... [more ▼]

Attempts to develop liver support systems for the treatment of patients with liver failure have ranged from use of plasma exchange to utilization of charcoal columns and extracorporeal devices loaded with liver tissue. However, no system has achieved wide clinical use and - in the absence of liver transplantation - severe hepatic failure continues to be associated with significant morbidity and mortality. In this paper, the authors review the current status of liver assist systems and summarize their clinical experience with a xenogeneic cell based-bioartificial liver. [less ▲]

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See detailStress coupling between earthquakes in Northwest Turkey and the North Aegean Sea
Nalbant, Süleyman; Hubert, Aurelia ULg; King, G. C. P.

in Journal of Geophysical Research (1998), 103

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See detailAutonomy to plant growth regulators and gene expression in periwinkle cultures in vitro
Droual, Anne-Marie; Hamdi, Said; Creche, Joel et al

in Journal of Plant Physiology (1998), 153(5-6), 623-630

To better understand the effect of habituation on gene expression in plant cells, we have compared the accumulation of specific mRNAs encoding respectively two proline-rich proteins, a chaperone protein ... [more ▼]

To better understand the effect of habituation on gene expression in plant cells, we have compared the accumulation of specific mRNAs encoding respectively two proline-rich proteins, a chaperone protein and three enzymes linking primary and secondary metabolisms in two models of in vitro culture of periwinkle. These models consisted of two couples of a 2,4-dichlorophenoxyacetic acid-dependent/2,4-dichlorophenoxyacetic acid independent line in which autonomy to auxin and cytokinin was obtained either through habituation or through transformation with the isopentenyltransferase gene from Agrobacterium tumefaciens. Results showed that gene expression was modified by plant growth regulator autonomy but differently according to the type of autonomy: only the gene encoding a hydroxyproline-rich glycoprotein was regulated similarly in both PGR-independent lines. On the other hand, PGR autonomy did not lead to total insensitivity to exogenously-applied PGRs, and the two PGR autonomous lines did not accumulate indole alkaloids for different reasons. [less ▲]

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See detailComment j'explore ... la néphropathie diabétique. Deuxième partie: suivi de la filtration glomérulaire
Weekers, Laurent ULg; Scheen, André ULg; Godon, J. P.

in Revue Médicale de Liège (1998), 53(9), 571-5

The natural history of diabetic renal disease can be divided into different stages according to the degree of albuminuria and the level of glomerular filtration rate (GFR). Assessment of the early ... [more ▼]

The natural history of diabetic renal disease can be divided into different stages according to the degree of albuminuria and the level of glomerular filtration rate (GFR). Assessment of the early hyperfiltration state, as well as determination of the rate of decline in kidney function at a later stage require precise and accurate methods to measure GFR. In this article, we briefly remind the reader of the physiological basis of GFR. We then review the different techniques for the monitoring of kidney function (inulin clearance, endogenous creatinine clearance, plasma disappearance of a radiolabelled tracer) and discuss the pro and cons of each of them in different clinical settings. Finally, we try to define a rational use of these techniques in everyday clinical practice. [less ▲]

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See detailPurification of myeloperoxidase from equine polymorphonuclear leucocytes.
Mathy, Marianne ULg; Bourgeois, E.; Grulke, Sigrid ULg et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1998), 62(2), 127-32

Increases of plasma concentrations of neutrophil myeloperoxidase (MPO) can be used as markers of polymorphonuclear leucocytes (PMN) activation in pathological situations (sepsis, acute lung injury, acute ... [more ▼]

Increases of plasma concentrations of neutrophil myeloperoxidase (MPO) can be used as markers of polymorphonuclear leucocytes (PMN) activation in pathological situations (sepsis, acute lung injury, acute inflammation). To develop an assay for measurement of plasma MPO in horses during the above-mentioned infectious and inflammatory conditions, MPO was purified from equine PMN isolated from blood anticoagulated with citrate. PMN were extracted in a saline milieu (0.2 M Na acetate, 1 M NaCl, pH 4.7) to eliminate most of cellular proteins. Pellets were then extracted in the same buffer containing cationic detergent (1% cetyltrimethyl ammonium bromide). The supernatant was further purified by ion exchange chromatography (Hiload S Sepharose HP column 0.5 x 26 cm, equilibrated with 25 mM Na acetate, 0.2 M NaCl, pH 4.7) with a NaCl gradient (until 1 M). Most of the peroxidase activity of MPO (spectrophotometrically measured by the oxidation of orthodianisidine by hydrogen peroxide) was eluted at 0.65 M NaCl. MPO was further purified by gel filtration chromatography (Sephacryl S 200 column 2.6 x 42 cm with 25 mM Na acetate, 0.2 M NaCl, pH 4.7). MPO (specific activity: 74.3 U/mg) was obtained with a yield of 30% from the detergent extraction supernatant. Electrophoresis (non-reducing conditions) showed 3 bands identified, by comparison with human MPO, (i) the mature tetrameric enzyme (150 kDa) with 2 light and 2 heavy subunits, (ii) the precursor form (88 kDa) and (iii) a form of the heavy subunit without the prosthetic heme group (40 kDa). The mature enzyme and its precursor were glycosylated and possessed peroxidase activity. Equine MPO showed strong similarities with human and bovine MPO, with an absorption peak at 430 nm (Soret peak) characteristic of ferrimyeloperoxidase. Enzymatic activity was pH dependent (optimal value at pH 5.5). [less ▲]

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See detailPathogenesis of abdominal aortic aneurysm (AAA) formation.
Limet, Raymond ULg; Richelle, Betty ULg; VERLOES, Alain ULg et al

in Acta Chirurgica Belgica (1998), 98(5), 195-8

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See detailAntibody formation and plasma profiles of insulin-like growth factor-I (IGF-I) and IGF-binding proteins in growing heifers after sustained-release exogenous BST administration
Bertozzi, Carlo; Portetelle, Daniel ULg; Massart, Serge et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1998), 2(special issue), 45

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See detailSuccessful Bridge to Transplantation with Pierce Donachy (Thoratec) Ventricular Assist Device
DEFRAIGNE, Jean ULg; DETRY, Olivier ULg; Demoulin, J.C. et al

in Acta Chirurgica Belgica (1998), 98(2), 90-4

Biventricular assistance with the Thoratec ventricular assist device was performed in a 48-year-old man waiting heart transplantation since 6 months. Indication for circulatory support was considered ... [more ▼]

Biventricular assistance with the Thoratec ventricular assist device was performed in a 48-year-old man waiting heart transplantation since 6 months. Indication for circulatory support was considered because of the development of progressive irreversible right heart failure with deterioration of the hepatic and renal functions. Orthotopic heart transplantation was performed after 13 days of circulatory support. No complications occurred during the assistance and in the posttransplant period. The patient was discharged on day 21 after transplantation. Five months after transplantation he is well and alive. [less ▲]

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See detailLe Global Positioning System au service des sciences de la Terre
Warnant, René ULg

in Ciel et Terre (1998), 114(6), 166-173

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See detailCompétence vectorielle des mouches ténérables de Glossina morsitans morsitans (Mall) infectées parTrypanosoma congolense (Nannomonas) IL 1180
Kazadi, J.-M.; Kagéruka, P.; Losson, Bertrand ULg et al

in Insect Science and its Application (1998), 18

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See detailCase Management voor geriatrische patienten en hun families : Het klinisch model van belgische projecten
Borgermans, L.; Abraham, I.; Milisen, K. et al

in Tijdschrift voor de Geschiedenis der Geneeskunde, Natuurwetenschappen, Wiskunde en Techniek (1998), 54(6), 427-435

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See detailNotre école offre-t-elle à tous les mêmes occasions d'apprendre?
Crahay, Marcel ULg

in Scientia Paedagogica Experimentalis (1998), 35(2), 381-418

Cet article présente les données de six études portant sur la gestion du temps scolaire en Communauté française de Belgique. Trois d'entre elles ont été menées dans des classes maternelles, deux dans des ... [more ▼]

Cet article présente les données de six études portant sur la gestion du temps scolaire en Communauté française de Belgique. Trois d'entre elles ont été menées dans des classes maternelles, deux dans des classes primaires et une au niveau secondaire. Les données ont été recueillies par observation directe dans cinq études et par l'intermediaire de rapports d'enseignant dans la sixième. L'analyse des résultats montre l'importante variabilité inter-classes du temps alloué aux différentes activités éducatives. Ce constat est valable pour tous les niveaux d'enseignement enquêtes. D'autres conclusions sont spécifiques au niveau d'enseignement. Au niveau préscolaire, il existe peu d'activités communes à toutes les classes (Ceci signifie que la nature du programme applique varie d'une classe a l'autre.) Au niveau primaire bien que le temps alloué aux différentes disciplines scolaires varie énormement d'une classe a l'autre, certaines tendances se dégagent : tous les enseignants consacrent aux mathématiques un temps superieur à ce qui est recommandé par le programme; l'enseignement de la grammaire débute en deuxieme année du primaire, alors que le programme fait démarrer cet apprentissage plus tard ; l'enseignement des sciences est negligé partout. Au niveau secondaire, on observe que les enseignants utilisent le temps de façon moins efficace dans les sections professionnelles que dans les sections générales. L'auteur avance que des systèmes de pilotage du rendement scolaire et des formules utilisant l'évaluation externe a des fins de régulation pourraient constituer une garantie d'égalité. [less ▲]

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See detailMating system of Calluna vulgaris (L.) Hull : self-sterility and outcrossing estimations
Mahy, Grégory ULg; Jaqcuemart, Anne Laure

in Canadian Journal of Botany (1998), 76

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See detailThe prevalence of Clostridium perfringens in the horse
Griffiths, N. J.; Walton, J. R.; Edwards, G. B. et al

in Rev Med Microbiol (1998), 9

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See detailRégulation transcriptionnelle du gène de la prolactine humaine
Muller, Marc ULg; Berwaer, Monique; Caccavelli, Laure et al

in Medecine Sciences : M/S (1998), 14(580-587),

Le gène humain de la prolactine (hPRL) est exprimé essentiellement par l'antéhypophyse. L'analyse des éléments régulateurs de la transcription sur plus de 5 000 bases en amont du site de début de ... [more ▼]

Le gène humain de la prolactine (hPRL) est exprimé essentiellement par l'antéhypophyse. L'analyse des éléments régulateurs de la transcription sur plus de 5 000 bases en amont du site de début de transcription a montré l'importance du contrôle par le facteur de transcription Pit-1, spécifique de l'hypophyse, à côté de facteurs ubiquistes. Des hormones modulent l'expression du gène hPRL, transmettant leur signal par les voies intracellulaires de l'AMP cyclique et du calcium, relayées au niveau du promoteur proximal (-250/+1) essentiellement par les facteurs de transcription Pit-1 et AP-1. Les récepteurs nucléaires contrôlent aussi en partie la transcription de hPRL: le récepteur des oestrogènes l'active en se liant aux éléments de réponse distaux ; les récepteurs nucléaires des hormones thyroïdiennes et des glucocorticoïdes la répriment en interférant respectivement avec la fonction activatrice de AP-1 et de Pit-1. [less ▲]

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See detailTumour necrosis factor (TNF) gene polymorphism influences TNF-alpha production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans.
Louis, Edouard ULg; Franchimont, D.; Piron, Anne ULg et al

in Clinical & Experimental Immunology (1998), 113(3), 401-406

TNF-alpha is involved in infectious and immuno-inflammatory diseases. Different individuals may have different capacities for TNF-alpha production. This might determine a predisposition to develop some ... [more ▼]

TNF-alpha is involved in infectious and immuno-inflammatory diseases. Different individuals may have different capacities for TNF-alpha production. This might determine a predisposition to develop some complications or phenotypes of these diseases. The aims of our study were to assess the inter-individual variability of TNF-alpha production and to correlate this variability to a single base pair polymorphism located at position -308 in TNF gene. We studied 62 healthy individuals. TNF-alpha production after LPS stimulation was evaluated using a whole blood cell culture model. The TNF gene polymorphism was studied by an allele-specific polymerase chain reaction. Other cytokines produced in the culture, soluble CD14 concentrations and expression of CD14 on blood cells were also measured. Among the 62 individuals, 57 were successfully genotyped. There were 41 TNF1 homozygotes and 16 TNF1/TNF2 heterozygotes. TNF-alpha production after LPS stimulation of whole blood cell culture was higher among TNF2 carriers than among TNFI homozygotes (929pg/ml (480-1473pg/ml) versus 521 pg/ ml (178-1307 pg/ml); P<0.05). This difference was even more significant after correction of TNF-alpha production for CD14 expression on blood cells. In conclusion, the single base pair polymorphism at position -308 in the TNF gene may influence TNF-alpha production in healthy individuals. [less ▲]

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See detailDécouverte laparoscopique d'une leiomyomatose péritonéale disséminée: une étroite ressemblance avec la carcinose péritonéale métastatique
Heidebroek, A.; Gielen, J. L.; Kolh, Philippe ULg et al

in Annales de Chirurgie (1998), 52(3), 298-300

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See detailRimes et action dramatique dans la tragédie française : Racine, Corneille, Hugo
Evrard, Etienne ULg; Purnelle, Gérald ULg

in Revue. Informatique et Statistique dans les Sciences humaines (1998), 33

This paper studies eleven tragedies by Racine, four by Comeille and two by Hugo. All of them are written with the twelve-syllabics verse called "alexandrin". The system of rhymes associates the verses by ... [more ▼]

This paper studies eleven tragedies by Racine, four by Comeille and two by Hugo. All of them are written with the twelve-syllabics verse called "alexandrin". The system of rhymes associates the verses by pairs, which form kinds of distichs. We focus on the relation between the ends of the distichs and the ends of the characters' statcments: do the latter match the end of a distich, or the first verse of it, or do they appear in the middle of a verse? This feature is not random: in Racine and Corneille the ends of distichs and of statemenls match significantly, though Hugo avoids this feature more frequently. After a study of short statements (less than two verses)—which are more numerous in Hugo than in both classical tragedians—we find that, in Racine and Corneille, specific textual en dramatical effects, like questions, appear often when the end of a long statement is not associated with the end of a distich. At the opposite, when a question is a rhetorical question, it ends more frequently with the second verse of a distich. These facts don't occur in Hugo. [less ▲]

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See detailQualitative and quantitative evaluation of bisindole usambarane alkaloids in Strychnos usambarensis roots by high performance liquid chromatography diode-array
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

in Phytochemical Analysis (1998), 9(2), 63-66

A high performance liquid chromatographic method for the simultaneous determination of bisindole usambarane alkaloids from the roots of Strychnos usambarensis using a diode-array detector is proposed, The ... [more ▼]

A high performance liquid chromatographic method for the simultaneous determination of bisindole usambarane alkaloids from the roots of Strychnos usambarensis using a diode-array detector is proposed, The system, consisting of a RP-8 Select B column and a linear gradient elution with acetonitrile and acetate buffer allowed the separation of all the alkaloids in 20 min, Usambarensine was found to be the major tertiary alkaloid present in the roots, In order to demonstrate the possibility of quantifying the bisindole alkaloids in crude roots extracts, the quantitative evaluation of 3',4'-dihydrousambarensine, usambarensine and N-b-methylusambarensine was carried out. (C) 1998 John Wiley & Sons, Ltd. [less ▲]

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See detailL'enfant obèse: actualités.
Guillaume, Michèle ULg; Van Aelst, C.; Burniat, W.

in ONE (1998)

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See detailLes mycoplasmes respiratoires des bovins : II. Propriétés de virulence et vaccination
Mainil, Jacques ULg; Thomas, A.; Linden, Annick ULg

in Annales de Médecine Vétérinaire (1998), 142

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See detailAtlas du recensement : une lecture plurielle de la Wallonie
Merenne-Schoumaker, Bernadette ULg

in Revue du Conseil Economique et Social de la Région wallonne (1998), (52), 31-35

Présentation de la Wallonie à la lumière du recensement de 1991. Cinq traits majeurs sont mis en évidence : - une dualité Flandre)-Wallonie réelle mais non dominante, - un processus majeur : l ... [more ▼]

Présentation de la Wallonie à la lumière du recensement de 1991. Cinq traits majeurs sont mis en évidence : - une dualité Flandre)-Wallonie réelle mais non dominante, - un processus majeur : l'urbanisation, - le poids du passé industriel, - la dualisation de la société, - des modèle culturels nouveaux [less ▲]

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See detailEpidermal Calprotectin Expression in Lymphocyte-Depleted Cutaneous Graft-versus-Host Reaction
Pierard, Gérald ULg; Nikkels, Nazli ULg; Nikkels, Arjen ULg et al

in Archivos Argentinos de Dermatologia (1998), 48

One of the most important complications associated with bone marrow transplantation (BMT) is graft-versus-host reaction (GVHR) altering different organs. The immunosuppressive regimen frequently abates ... [more ▼]

One of the most important complications associated with bone marrow transplantation (BMT) is graft-versus-host reaction (GVHR) altering different organs. The immunosuppressive regimen frequently abates the severity of cutaneous lesions to a peculiar lymphocyto-depleted GVHR (LD-GVHR) with scant recognizable inflammatory cells and almost absence of epidermal injury. The recently revisited histological criteria for cutaneous GVHR are of little help in diagnosing such LD-GVHR. As calprotectin (L1-protein) has been reported to be expressed in several types of stressed epithelia, we assessed the epidermal calprotectin expression during LD-GVHR. Calprotectin expression was studied by immuno-histochemistry using the Mac 287 moAb in 50 cases of LD-GVHR and 40 cases of toxic reactions due to the conditioning regimens or to post-transplant drugs. Calprotectin was evidenced in normal looking keratinocytes of all cutaneous LD-GVHR cases and in the vast majority of cytotoxic drug-induced dermatitis. It is concluded that calprotectin immunoreactivity appears to be a diagnostic clue in LD-GVHR. The epidermal calprotectin expression occurs early in GVHD, irrespective of the histological grading. However, it cannot be used alone to distinguish early LD-GVHR from drug-induced dermatitis. [less ▲]

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See detailEvolution of the Peierls distortion in liquid AsxSb1-x compounds
Raty, Jean-Yves ULg; Gaspard, Jean-Pierre ULg; Céolin, R. et al

in Journal of Non-Crystalline Solids (1998), 232-234

The local order in the liquid binary alloy AsxSb1-x (0 ≤ x ≤ 1) is studied by short-wavelength (λ = 0.7 Å) neutron scattering. The coordination number (Z) and the interatomic distances (d) are analyzed in ... [more ▼]

The local order in the liquid binary alloy AsxSb1-x (0 ≤ x ≤ 1) is studied by short-wavelength (λ = 0.7 Å) neutron scattering. The coordination number (Z) and the interatomic distances (d) are analyzed in terms of the As concentration: Z increases continuously, from a value of 3.5 for pure arsenic to a value of 6.3 for pure antimony, whereas d shows a departure from Vegard's rule. The interatomic distance increases in the range 0.15 ≤ x ≤ 1.00 and is almost constant in the range 0.00 ≤ x ≤ 0.15. A simple tight-binding model is developed, which indicates that the relevant parameter is the hardness of the core repulsion between the atoms. © 1998 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailEnantioseparation of Acidic Drugs by Capillary Electrophoresis Using Dual Systems with Mixtures of Charged and Neutral Cyclodextrins
Fillet, Marianne ULg; Fotsing, Lucas ULg; Schomburg, G. et al

in Biomedical Chromatography : BMC (1998), 12(3, May-Jun), 131-2

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See detailIs the woodmouse (Apodemus sylvaticus) of sicily a distinct species?
Michaux, Johan ULg; Sara, Maurizio; Libois, Roland ULg et al

in Belgian Journal of Zoology (1998), 128(2), 211-214

[No abstract available]

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See detailLiver abnormalities in severely obese subjects: effect of drastic weight loss after gastroplasty.
Luyckx, Françoise ULg; Desaive, Claude ULg; Thiry, Albert ULg et al

in International Journal of Obesity & Related Metabolic Disorders (1998), 22(3), 222-6

OBJECTIVE: To examine the factors associated with liver steatosis in severely obese subjects and to test the potential reversibility of fatty liver after weight loss. DESIGN: Retrospective clinical study ... [more ▼]

OBJECTIVE: To examine the factors associated with liver steatosis in severely obese subjects and to test the potential reversibility of fatty liver after weight loss. DESIGN: Retrospective clinical study. SUBJECT: 528 obese patients before bariatric surgery and 69 obese subjects of the initial cohort evaluated before and 27+/-15 months after gastroplasty. MEASUREMENTS: Fatty deposition (scored as mild, moderate or severe) and inflammatory changes were evaluated in liver biopsies; clinical (body mass index (BMI), age, gender, duration of obesity) and biological (glucose, triglycerides, liver enzymes) parameters were related to histological findings. RESULTS: 74% of the 528 biopsies showed fatty change, estimated as mild in 41% of cases, moderate in 32% and severe in 27%. The prevalence of steatosis was significantly higher in men than in women (91% vs 70%, P = 0.001) and in patients with impaired glucose tolerance or type 2 diabetes compared with nondiabetics (89% vs 69% P = 0.001). The severity of the steatosis was associated with BMI (P = 0.002) but not with the duration of obesity or the age of the patient. When compared with patients without fatty change, those with liver steatosis had significantly higher fasting plasma glucose (5.5 mmol/l vs 5.1 mmol/l, P = 0.007) and triglycerides (1.8 mmol/l vs 1.3 mmol/l, P = 0.002). Mean serum liver enzyme activities (alkaline phosphatase, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl-transpeptidase (gammaGT) were significantly (P < 0.001) increased in patients with fatty change but remained within laboratory reference values. In the 69 patients who have been evaluated after a marked weight reduction (-32+/-19kg), 45% of the biopsies were considered as normal (vs 13% before, P < 0.001) while pure fatty change was still observed in 38% of the patients (vs 83% before, P = 0.001). However, the severity of the steatosis was significantly (P < 0.001) reduced (mild: 62% vs 21%; moderate: 23% vs 37%; severe: 15% vs 42%). In addition, a significant increase of hepatitis was observed in 26% of the biopsies (vs 14% before, P < 0.05). CONCLUSIONS: Liver steatosis in obese subjects is associated with men, diabetic status, BMI, higher fasting glucose and hypertriglyceridaemia. Postgastroplasty weight loss reduces liver steatosis, but seems to increase the incidence of inflammatory lobular hepatitis. [less ▲]

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See detailEléments pour une préhistoire transcendantale
de Villers Grand Champs, Bénédicte ULg

in Recherches Husserliennes (1998), 6

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See detailUne nouvelle grammaire de néo-égyptien
Winand, Jean ULg

in Orientalistische Literaturzeitung (1998), 93

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See detailLocalisation de la laminine, fibronectine, de la E-cadhérine et des intégrines dans l’endomètre et l’endométriose
BELIARD, Aude ULg; Donnez, J; Nisolle, Michelle ULg et al

in Reproduction Humaine et Hormones (1998), 2

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See detailSciences et Médecine à Liège à l'aube du troisième millénaire
Legros, Willy ULg; Rentier, Bernard ULg

in M S-Médecine Sciences (1998), 14(5), 535-536

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See detailQuel est votre diagnostic radiographique? Un cas de myélome multiple chez un chien.
Godefroid, T.; Sauders, J.; Peeters, Dominique ULg

in Annales de Médecine Vétérinaire (1998), 142

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See detailWiktor stent for treatment of chronic total coronary artery occlusions: short- and long-term clinical and angiographic results from a large multicenter experience.
Anzuini, A.; Rosanio, S.; Legrand, Victor ULg et al

in Journal of the American College of Cardiology (1998), 31(2), 281-8

OBJECTIVES: This study reports the first multicenter experience with the Wiktor coil stent for treatment of chronic total coronary artery occlusions (CTOs). BACKGROUND: Percutaneous transluminal coronary ... [more ▼]

OBJECTIVES: This study reports the first multicenter experience with the Wiktor coil stent for treatment of chronic total coronary artery occlusions (CTOs). BACKGROUND: Percutaneous transluminal coronary angioplasty (PTCA) of CTO is associated with very high restenosis and reocclusion rates. Coronary stenting has been proposed as a means of improving outcome. However, the Wiktor device for CTOs has never been tested in a large patient sample. METHODS: From January 1993 to December 1996, 89 patients with 91 CTOs underwent Wiktor stent implantation after successful PTCA. The post-stenting regimen consisted of warfarin (Coumadin) plus aspirin in the initial 49 patients (55%) and aspirin plus ticlopidine in 40 patients (45%). RESULTS: Stenting was successful in 87 patients (98%). At 1 month, 6% of patients had subacute stent thrombosis, 3% had a major bleeding event, and 1% had access-site complications. Subacute stent thrombosis showed univariate association with warfarin therapy (p = 0.009). Angiographic follow-up was obtained in 76 (93%) of 82 eligible patients. The restenosis rate was 32%, including 4% reocclusions. By multiple logistic regression analysis, restenosis was independently associated with multiple stents (adjusted odds ratio [OR] 27.67, 95% confidence interval [CI] 4.25 to 79.95, p = 0.0008) and increasing values of occlusion length (adjusted OR 1.23, 95% CI 1.09 to 1.39, p = 0.001). Freedom from death, myocardial infarction or stented vessel revascularization was 87% and 72% at 1 and 3 years, respectively. CONCLUSIONS: Short- and long-term clinical and angiographic outcomes are favorable in patients undergoing Wiktor stent implantation in CTO. Further technical improvement is needed to reduce the restenosis rate in patients with long lesions and multiple stents. [less ▲]

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See detailIntrathecal clonidine and fentanyl with hyperbaric bupivacaine improves analgesia during cesarean section
Benhamou, Dan; Schneider, Markus; Brichant, Jean-François ULg et al

in Anesthesia and Analgesia (1998), 87

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See detailProposal of boundaries for subdivision of the Famennian Stage: miospore implications.
Streel, Maurice ULg; Loboziak, S

in Newsletter. Subcommission on Devonian Stratigraphy (1998), 15

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See detailL'échangeur, exercice structuré en dynamique des groupes
Faulx, Daniel ULg

in Cahiers Internationaux de Psychologie Sociale (1998), 3

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See detailA further new species of Monoblastia (lichenized ascomycetes: Monoblastiaceae) from Papua New Guinea
Sérusiaux, Emmanuël ULg; Aptroot, André

in Nova Hedwigia (1998), 67

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See detailDifferential growth dependency of normal and habituated sugarbeet cell lines upon endogenous ethylene production and exogenous ethylene application
Bisbis, Badia; Kevers, Claire ULg; Creche, Joel et al

in Physiologia Plantarum (1998), 103(2), 201-208

A fully habituated (auxin- and cytokinin-independent) nonorganogenic (HNO) sugarbeet (Beta vulgaris) callus produces very little ethylene as compared with a normal (N) hormone-requiring callus of the same ... [more ▼]

A fully habituated (auxin- and cytokinin-independent) nonorganogenic (HNO) sugarbeet (Beta vulgaris) callus produces very little ethylene as compared with a normal (N) hormone-requiring callus of the same strain. Both callus types react by growth changes to application of inhibitors of ethylene biosynthesis and ethylene action, of 1-aminocyclopropane-1-carboxylic acid (ACC) as the immediate precursor of ethylene, to transfer from light to darkness, and also to application of exogenous ethylene or an ethylene trapper. This indicates their growth dependency upon their endogenously biosynthesized ethylene and also their sensitivity to exogenous gas. However, the sensitivity was generally higher for the HNO callus producing naturally less ethylene. The weaker reaction of the HNO callus to the exogenous ethylene was attributed to its hyperhydric status (a water layer surrounding the cells). Because low ethylene production appears as a general characteristic of habituated cell lines, the causal and/or consequential relationships of this low ethylene production with other characteristics of habituated tissues (absence of exogenous hormones in the culture media, deficiency of cell differentiation, accumulation of polyamines in neoplastic tissues) are discussed. [less ▲]

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See detailIncreased aortic compliance maintains left ventricular performance at lower energetic cost
Kolh, Philippe ULg; LAMBERMONT, Bernard ULg; GERARD, P et al

in European Heart Journal (1998), 19

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See detailClinical Vignette. Tetralogy of Fallot Associated with a Patent Ductus Arteriosus in a German Shepherd Dog
Mc Entee, Kathleen ULg; Snaps, Frédéric ULg; Clercx, Cécile ULg et al

in Journal of Veterinary Internal Medicine (1998), 12(1, Jan-Feb), 53-5

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See detaille corps et son interprète
Gauthier, Jean-Marie ULg

in Revue Belge de Psychanalyse (1998)

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See detailUtilisation des images satellitaires à très haute résolution pour la mise en œuvre de la révision des plans de secteur
Binard, Marc ULg; Nadasdi, Isvan

in Bulletin de la Société Géographique de Liège (1998), 34

Research, on the one hand, confirms the availability of land for additional housing developments, and on the other reveals the consequences of bad urbanization policies (promotion and development of ... [more ▼]

Research, on the one hand, confirms the availability of land for additional housing developments, and on the other reveals the consequences of bad urbanization policies (promotion and development of linear housing developments, insufficient control over quarrying activities, etc.). The "new code" introduces new town planning requirements (more detailed zoning analysis taking into account the environmental aspects, creation of protected areas, impact surveys, ground occupancy estimation) and strengthens the prospect of using this methodology further on. [less ▲]

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See detailDeux nouvelles espèces de Byssoloma Trev. (lichens Pilocarpaceae) d'Europe Occidentale et de Macaronésie
Sérusiaux, Emmanuël ULg

in Cryptogamie. Bryologie, Lichenologie (1998), 19

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See detailbébé prématuré, bébé particulier ? Quel suivi développemental ?
Battisti, Oreste ULg; François, A.; Bertrand, J. M. et al

in Archives de Pédiatrie (1998), 5

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See detailAutocrine role of TGFbeta1 in adrenal.
Langlois, D.; Leroy, Céline ULg; Penhoat, A. et al

in Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme (1998), 30(6-7), 411-415

Three main criteria are required to postulate that a factor has an autocrine action in any cell: presence of receptors and biological effects of the factor; local production which must be regulated by ... [more ▼]

Three main criteria are required to postulate that a factor has an autocrine action in any cell: presence of receptors and biological effects of the factor; local production which must be regulated by physiological signals and the inhibition of its secretion or action should cause effects opposite to those caused by the exogenous factor. In adrenal cells, transforming growth factor beta1 (TGFbeta1) fulfilled these criteria: 1) Adrenal cells possess TGFbeta1 receptors and this factor regulates the expression of adrenal cell specific genes, including ACTH and angiotensin II (AngII) receptors and several steroidogenic enzymes, leading to changes in the steroidogenic responsiveness; 2) Adrenal cells express and secrete TGFbeta1 and its secretion is regulated by the two adrenal specific hormones ACTH and AngII; 3) The blockade of TGFbeta1 protein synthesis by an antisense oligonucleotide produces changes in adrenal cell functions opposed to those induced by the exogenous factor. [less ▲]

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See detailCalibration of the Internal Energy Distribution of Ions Produced by Electrospray
Collette, Caroline ULg; De Pauw, Edwin ULg

in Rapid Communications in Mass Spectrometry : RCM (1998), 12

The internal energy deposited in the ions in the source of mass spectrometer governs their fragmentation and therefore the content of the spectra. When the ionization conditions are well defined and ... [more ▼]

The internal energy deposited in the ions in the source of mass spectrometer governs their fragmentation and therefore the content of the spectra. When the ionization conditions are well defined and reproductible, the elaboration of databases benefits the use of the method. In electrospray, however, the source conditions are not strictly defined. The elaboration of spectra databases therefore requires a calibration of internal energy of the ions. In this article, a method for the calibration of the internal energy of the ions in electrospray is presented, developed using fragmentation reactions of a set of probe ions (benzylpyridinium salts) under various conditions. [less ▲]

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See detailDesign of an axial mechanical gas seal with self-acting lift geometry
DOCQUIER, N.; NELIS, M.; Bozet, Jean-Luc ULg

in European Journal of Mechanical and Environmental Engineering [=EJMEE] (1998), 43(no4), 195-203

Cet article étudie le comportement d'un joint mécanique sans contact à faces radiales, doté d'un dispositif d'augmentation de portance. La répartition des pressions et des vitesses dans le film mince est ... [more ▼]

Cet article étudie le comportement d'un joint mécanique sans contact à faces radiales, doté d'un dispositif d'augmentation de portance. La répartition des pressions et des vitesses dans le film mince est établie grâce à l'équation de Reynolds résolue au moyen de différences finies. L'étude paramétrique des caractéristiques du joint est ensuite réalisée en vue d'en déduire des abaques de fonctionnement et, par la suite, une démarche cohérente de choix des dimensions. L'auteur présente donc tout d'abord le fonctionnement d'un joint mécanique à gaz et il en décrit la géométrie et les conditions de travail. Après avoir défini et justifié les hypothèses utilisées, il propose une mise en équation du problème et décrit le comportement du système d'étanchéité en régime au moyen des résultats obtenus. L'article présente finalement l'influence de la géométrie du joint et propose une méthode de dimensionnement. [less ▲]

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See detailCloches et carillons dans les Principautés de Liège et Stavelot-Malmedy. Richesse et actualité du patrimoine campanaire
Marganne, Marie-Hélène ULg; Mélard, Marc

in Feuillets de la Cathédrale de Liège (1998), 33-38

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See detailVingt ans de rénovation rurale en Belgique wallonne
Schmitz, Serge ULg; Christians, Charles ULg

in Cadernos de Geografia (1998), 17

The paper analyses the twenty years of rural development policy in Wallonia and looks for its impact in the countryside renewal.

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See detailLes encéphalopathies spongiformes et leurs répercussions réglementaires
Pastoret, Paul-Pierre ULg; Falize, Françoise; Brochier, Bernard et al

in Annales de Médecine Vétérinaire (1998), 142

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See detailInfluence of Electrolytes on Liquid-Liquid Extraction
Pfennig, Andreas ULg; Schwerin, Albrecht

in Industrial & Engineering Chemistry Research (1998), 37(8), 3180-3188

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See detailFinite Element Comparative Study of Ship Structural Details
Iwahashi, Y.; Sumi, Y.; Hu, T. et al

in Marine Structures (1998)

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See detailNorthern and southern hemisphere ground-based infrared spectroscopic measurements of tropospheric carbon monoxide and ethane
Rinsland, C. P.; Jones, N. B.; Connor, B. J. et al

in Journal Of Geophysical Research. Atmospheres (1998), 103(D21), 28197-28217

Time series of CO and C2H6 measurements have been derived from high-resolution infrared solar spectra recorded in Lauder, New Zealand (45.0 degrees S, 169.7 degrees E, altitude 0.37 km), and at the U.S ... [more ▼]

Time series of CO and C2H6 measurements have been derived from high-resolution infrared solar spectra recorded in Lauder, New Zealand (45.0 degrees S, 169.7 degrees E, altitude 0.37 km), and at the U.S. National Solar Observatory (31.9 degrees N, 111.6 degrees W, altitude 2.09 km) on Kitt Peak. Lauder observations were obtained between July 1993 and November 1997, while the Kitt Peak measurements were recorded between May 1977 and December 1997. Both databases were analyzed with spectroscopic parameters that included significant improvements for C2H6 relative to previous studies. Target CO and C2H6 lines were selected to achieve similar vertical samplings based on averaging kernels. These calculations show that partial columns from layers extending from the surface to the mean tropopause and from the mean tropopause to 100 km are nearly independent. Retrievals based on a semiempirical application of the Rodgers optimal estimation technique are reported for the lower laver, which has a broad maximum in sensitivity in the upper troposphere. The Lauder CO and C2H6 partial columns exhibit highly asymmetrical seasonal cycles with minima in austral autumn and sharp peaks in austral spring. The spring maxima are the result of tropical biomass burning emissions followed by deep convective vertical transport to the upper troposphere and long-range horizontal transport. Significant year-to-year variations are observed for both CO and C2H6, but the measured trends, (+0.37 +/- 0.57)% yr(-1) and (-0.64 +/- 0.79)% yr(-1), 1 sigma, respectively, indicate no significant long-term changes. The Kitt Peak data also exhibit CO and C2H6 seasonal variations in the lower layer with trends equal to (-0.27 +/- 0.17)% yr(-1) and (-1.20 +/- 0.35')% yr(-1), 1 sigma, respectively. Hence a decrease in the Kitt Peak tropospheric C2H6 column has been detected, though the CO trend is not significant. Both measurement sets are compared with previous observations, reported trends, and three-dimensional model calculations. [less ▲]

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See detailA discussion of methods for estimating residual fluxes in strong tidal estuaries
Regnier, P.; Mouchet, Anne ULg; Wollast, R. et al

in Continental Shelf Research (1998), 18

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See detailPathological investigations on guillemots (Uria aalge) stranded on the Belgian coast during the winter of 1993-94
Jauniaux, Thierry ULg; Brosens, L.; Meire, P. et al

in Veterinary Record : Journal of the British Veterinary Association (1998), 143(14), 387-390

Pathological investigations were carried out on 67 guillemots (Uria aalge) washed up on the Belgian coast between November 1993 and March 1994. Emaciation and acute haemorrhagic gastroenteropathy were ... [more ▼]

Pathological investigations were carried out on 67 guillemots (Uria aalge) washed up on the Belgian coast between November 1993 and March 1994. Emaciation and acute haemorrhagic gastroenteropathy were observed in more than 70 per cent of the birds. There was no statistical relationship between the level of oil contamination and the severity of the lesions. Differences in bodyweight were accounted for by age, sex, emaciation, and acute haemorrhagic gastroenteropathy. The birds had a severe weight deficit but the concentrations of pollutants were below acutely toxic levels. [less ▲]

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See detailLe curateur aux meubles et les scellés
Moreau, Pierre ULg

in Journal des Juges de Paix = Tijdschrift van de Vrederechters (1998), (8, 9, 10), 481-490

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See detailEquine neutrophil myeloperoxidase in plasma: design of a radio-immunoassay and first results in septic pathologies.
Deby, Ginette ULg; Grulke, Sigrid ULg; Caudron, I. et al

in Veterinary Immunology and Immunopathology (1998), 66(3-4), 257-71

The strangulated intestinal pathologies of horses are accompanied by a local activation of the neutrophils, that can be revealed by measuring the tissular enzymatic activity of the granulocytic enzyme ... [more ▼]

The strangulated intestinal pathologies of horses are accompanied by a local activation of the neutrophils, that can be revealed by measuring the tissular enzymatic activity of the granulocytic enzyme myeloperoxidase (MPO). To estimate the possible spreading of this neutrophil activation to the systemic circulation, we designed a radioimmunoassay (RIA) for equine neutrophil myeloperoxidase (MPO) (EC 1.11.1.7) using a specific rabbit antiserum. MPO was labeled with 1 mCi 125I by a technique of self-labeling in the presence of 10(-4) M hydrogen peroxide. The RIA was performed by incubation of 100 microl diluted antiserum, 100 microl labeled MPO (+/-30,000 cpm) and 100 microl of the reference molecule (unlabeled MPO) solution or the unknown sample, at room temperature for 18 h. The antibody-antigen complexes were isolated by double antibody precipitation. The sensitivity of the RIA was 2 ng/ml. The RIA showed good precision and accuracy with intra- and inter-assay coefficients of variation 6% and 8%, respectively, for MPO concentrations ranging from 2 ng/ml to 60 ng/ml. The best sampling technique for MPO measurement in plasma was to collect blood into EDTA, which allowed us to get a plasmatic value stable with time. The mean MPO value in normal horses was 69.5 +/- 19.4 ng/ml in EDTA anticoagulated plasma (n = 48). The stress of transport and anaesthesia did not modify the mean plasmatic value of MPO. No significant increase of plasma MPO was observed in 17 horses submitted to surgery for pathologies without systemic impact. But, in 25 horses with obstructive intestinal pathologies, persistent abnormal MPO concentrations were measured (until 740 ng/ml). [less ▲]

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See detailContrôle de la qualité des pommes par vision artificielle.
Leemans, Vincent ULg; Magein, Hugo ULg; Destain, Marie-France ULg

in Fruit Belge (1998), 66(471),

Article de vulgarisation à destination des profesionnels du secteur fruitier, présentant les possibilités de l'analyse d'images numériques pour le tri des fruits suivant leur qualité externe

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See detailAperçu des travaux du LASLA
Denooz, Joseph ULg

in Revue. Informatique et Statistique dans les Sciences Humaines (1998)

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See detailFurther observations on the lichen genus Strigula in New Zealand
Sérusiaux, Emmanuël ULg

in Bryologist (1998), 101

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See detailCompte-rendu de l'article de N. Zagagi, Divine Interventions and Human Agents in Menander
Renaud, Jean-Michel ULg

in Kernos : Revue Internationale et Pluridisciplinaire de Religion Grecque (1998), Supplément 6

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See detailStudy of siliceous karst genesis in eastern Niger : microscopy and X ray microanalysis of speleothems
Willems, Luc; Compère, Philippe ULg; Sponholz, Barbara

in Zeitschrift für Geomorphologie (1998), 42

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See detailEnseignants de français et d'histoire. Mieux se connaître pour mieux collaborer
Jadoulle, Jean-Louis ULg

in Français 2000 (1998), n°161-162

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See detailhigh frequency oscillatory ventilation in RDS
Battisti, Oreste ULg

in Pediatric Research (1998), 2

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See detailEffect of ice-nucleating bacteria (Pseudomonas syringae Van Hall) on insect susceptibility to sub-zero temperatures
Mignon, Jacques ULg; Haubruge, Eric ULg; Gaspar, Charles ULg

in Journal of Stored Products Research (1998), 34(1), 81-86

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits ... [more ▼]

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits the acclimation of insects and enhances their cold tolerance. The objective of this study was to examine the influence of the concentration of the ice-nucleating-active bacterium Pseudomonas syringae (10, 100 and 1000 ppm), temperature and duration of sub-zero exposure on the cold tolerance of the granary weevil Sitophilus granarius (L.) and the saw-toothed grain beetle Oryzaephilus surinamensis (L.). After an application of 1000 ppm of powdered P. syringae to grain, the mortality of S. granarius and O. surinamensis was increased after 24-h exposure to -4 degrees C. Higher mortality was observed after exposure to colder temperatures and a dose-response relationship was evident. At near-zero (-4 to 0 degrees C) negative temperatures, no dose response was observed, and the mortality in treated grain was the same as that in untreated grain. (C) 1998 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailApoptosis During the Development of Radiogenic Thymic Lymphomas: Effects of Treatments Inhibiting Lymphoma Development
Humblet, Chantal ULg; Denis, Ghislaine; Greimers, Roland ULg et al

in Anticancer Research (1998), 18(5A, Sep-Oct), 3469-74

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an ... [more ▼]

INTRODUCTION: Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which preleukemic cells progressively transform into leukemic cells within an abnormal thymic microenvironment. A bone marrow graft or repeated cytokine injections prevent lymphoma development. We think that these treatments restore altered mechanisms controlling apoptosis. MATERIALS AND METHODS: Apoptosis was analyzed by flow cytometry in thymocytes from different groups of mice (control, preleukemic, prevented mice). RESULTS: The apoptotic rates did not change in freshly isolated thymocytes from different experimental groups. However, after culture, the level of apoptosis increased in preleukemic thymuses; and returned to normal value in cultured thymocytes from irradiated mice after lymphoma preventing treatments. Furthermore, thymic microenvironmental factors can control thymocyte apoptosis. CONCLUSION: We propose that after leukemogenic irradiation, there is an increase of cells with an activated suicide program, but that alterations of thymic environmental factors rescue them from apoptosis, allowing their further neoplastic transformation. [less ▲]

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See detailComparison of the Internal Energy Distributions of Ions Produced by Different Electrospray Sources
Collette, Caroline ULg; Drahos, Laszlo; De Pauw, Edwin ULg et al

in Rapid Communications in Mass Spectrometry : RCM (1998), 12

The internal energies of the emitted ions can be modulated in an electrospray source through different experimental conditions. However, the fragmentation pattern depends also on conditions that cannot be ... [more ▼]

The internal energies of the emitted ions can be modulated in an electrospray source through different experimental conditions. However, the fragmentation pattern depends also on conditions that cannot be controlled by the operator. These differences make difficult the comparison of the electrospray mass spectra obtained with different mass spectrometers. A method for the calibration of the internal energies of ions produced by an electrospray source has been presented previously to study the influence of experimental conditions on the internal energies of the ions. In this work, new results were obtained with modified values of fragmentation energy taking into account the kinetic shift of thermometer ions. [less ▲]

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See detailStudy of exo-beta-1,3-glucanase activity production by the yeast Pichia anomala in relation to its antagonistic properties against Botrytis cinerea on postharvest apples.
Grevesse, C.; Jijakli, Mohamed ULg; Lepoivre, Philippe ULg

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1998), 63(4b), 1685-1682

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See detailAssociation of fibroblastoid features with the invasive phenotype in human bronchial cancer cell lines
Polette, M.; Gilles, Christine ULg; de Bentzmann, S. et al

in Clinical & Experimental Metastasis (1998), 16(2), 105-12

The acquisition of a metastatic phenotype by epithelial cells implicates a series of changes altering their differentiation, their overall behavior and morphology. In the present study, we have examined ... [more ▼]

The acquisition of a metastatic phenotype by epithelial cells implicates a series of changes altering their differentiation, their overall behavior and morphology. In the present study, we have examined the relationships between the cellular morphology, E-cadherin expression, matrix metalloproteinases expression and in vitro invasive properties in two human bronchial immortalized cell lines. The (16HBE14o-) cell line which did not show any invasive abilities in the Boyden chamber assay displayed a typical epithelial morphology in monolayer, expressed high levels of E-cadherin and synthesized neither MMP-2 and MT1-MMP nor vimentin. In contrast, the BZR cell line which was highly invasive displayed a more elongated phenotype in monolayer, did not produce E-cadherin but expressed vimentin, MMP-2 and MT1-MMP. Our data therefore suggest that the metastatic progression of broncho-pulmonary cancer cells results in a cellular dedifferentiation and the gain of some mesenchymal attributes (loss of E-cadherin and expression of vimentin) associated with enhanced degradative properties (expression of metalloproteinases) [less ▲]

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See detailLe genre Psilophyton dans le Dévonien inférieur de Belgique: P. cf. crenulatum Doran et P. forbesii Andrews, Kasper & Mencher.
Gerrienne, Philippe ULg

in Annales de la Société Géologique de Belgique (1998), 120

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See detailProtective activity of propofol, Diprivan and intralipid against active oxygen species.
Mathy, Marianne ULg; Deby, Ginette ULg; Hans, Pol ULg et al

in Mediators of Inflammation (1998), 7(5), 327-33

We separately studied the antioxidant properties of propofol (PPF), Diprivan (the commercial form of PPF) and intralipid (IL) (the vehicle solution of PPF in Diprivan) on active oxygen species produced by ... [more ▼]

We separately studied the antioxidant properties of propofol (PPF), Diprivan (the commercial form of PPF) and intralipid (IL) (the vehicle solution of PPF in Diprivan) on active oxygen species produced by phorbol myristate acetate (10(-6) M)-stimulated human polymorphonuclear leukocytes (PMN: 5 x 10(5) cells/assay), human endothelial cells (5 x 10(5) cells/assay) or cell-free systems (NaOCl or H2O2/peroxidase systems), using luminol (10(-4) M)-enhanced chemiluminescence (CL). We also studied the protective effects of Diprivan on endothelial cells submitted to an oxidant stress induced by H2O2/MPO system: cytotoxicity was assessed by the release of preincorporated 51Cr. Propofol inhibited the CL produced by stimulated PMN in a dose dependent manner (until 5 x 10(-5) M, a clinically relevant concentration), while Diprivan and IL were not dose-dependent inhibitors. The CL produced by endothelial cells was dose-dependently inhibited by Diprivan and PPF, and weakly by IL (not dose-dependent). In cell free systems, dose-dependent inhibitions were obtained for the three products with a lower effect for IL. Diprivan efficaciously protected endothelial cells submitted to an oxidant stress, while IL was ineffective. By HPLC, we demonstrated that PPF was not incorporated into the cells. The drug thus acted by scavenging the active oxygen species released in the extracellular medium. IL acted in the same manner, but was a less powerful antioxidant. [less ▲]

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See detailProjet Solidarité : Accompagnement d'équipes éducatives dans la réalisation de leur projet d'établissement
Lenoir, A.; Mouvet, Bernadette ULg; Munten, J. et al

in Point sur la Recherche en Education (Le) (1998), 8

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See detailPathologie bovine : transport de l’oxygène et solutions salines
Gustin, Pascal ULg; Cambier, Carole ULg; Frans, A. et al

in Agricontact (1998), 302

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See detailRésistance aux antibiotiques de souches d'Escherichia coli bovines et aviaires isolées en Algérie
Mellata, M.; Jacquemin, E.; Bakour, R. et al

in Annales de Médecine Vétérinaire (1998), 142

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See detailL'image du mois. Acne gymnasium: une acne fulminante dopee.
Pierard, Gérald ULg

in Revue medicale de Liege (1998), 53(8), 441-3

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See detailGrazing by large river zooplankton: a key to summer potamoplankton decline? The case of the Meuse and Moselle rivers in 1994 and 1995
Gosselain, Véronique; Descy, Jean-Pierre; Viroux, Laurent et al

in Hydrobiologia (1998)

To explain summer declines in phytoplankton biomass in large rivers, we compared the effect of zooplankton grazing on the planktonic algae of two large European rivers, the Meuse and the Moselle. In situ ... [more ▼]

To explain summer declines in phytoplankton biomass in large rivers, we compared the effect of zooplankton grazing on the planktonic algae of two large European rivers, the Meuse and the Moselle. In situ grazing was measured during two years (1994 and 1995), using the Haney method. Total zooplankton community filtration rates recorded in the river Meuse ranged between 1 and 32% of the water volume filtered per day. A drastic algal decline was observed early July both years and may be explained by high densities of a rotiferdominated zooplankton community (500–700 ind. l􀀀1) with more than 75%of Brachionus calyciflorus. During the summer period in 1994, when grazingwas over 20%, edible algal biomasswas controlled by a diversified rotifer community (up to 2500 ind.l􀀀1), while a nonedible algal assemblage developed. In contrast, phytoplankton biomass remained comparatively lowin the Moselle throughout the lowflowperiod, as did zooplankton numbers duringmost of this time (fewer than 200 ind. l􀀀1 during the summer period). The proportion of crustaceans in this zooplanktonwas rather higher than in the Meuse, and they dominated at times, in biomass as well as in numbers. Nevertheless, measured in situ grazing rates (1–15%) could not explain the low summer algal biomass, even if low filtration rates may at times represent a significant carbon loss for phytoplankton, when and where net algal production was low. As a conclusion, the role of phytoplankton – zooplankton interactions in controlling algal biomass in large rivers is discussed. [less ▲]

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See detailFormation of the 67-kDa laminin receptor by acylation of the precursor.
Buto, S.; Tagliabue, E.; Ardini, E. et al

in Journal of Cellular Biochemistry (1998), 69(3), 244-51

Even though the involvement of the 67-kDa laminin receptor (67LR) in tumor invasiveness has been clearly demonstrated, its molecular structure remains an open problem, since only a full-length gene ... [more ▼]

Even though the involvement of the 67-kDa laminin receptor (67LR) in tumor invasiveness has been clearly demonstrated, its molecular structure remains an open problem, since only a full-length gene encoding a 37-kDa precursor protein (37LRP) has been isolated so far. A pool of recently obtained monoclonal antibodies directed against the recombinant 37LRP molecule was used to investigate the processing that leads to the formation of the 67-kDa molecule. In soluble extracts of A431 human carcinoma cells, these reagents recognize the precursor molecule as well as the mature 67LR and a 120-kDa molecule. The recovery of these proteins was found to be strikingly dependent upon the cell solubilization conditions: the 67LR is soluble in NP-40-lysis buffer whereas the 37LRP is NP-40-insoluble. Inhibition of 67LR formation by cerulenin indicates that acylation is involved in the processing of the receptor. It is likely a palmitoylation process, as indicated by sensitivity of NP-40-soluble extracts to hydroxylamine treatment. Immunoblotting assays performed with a polyclonal serum directed against galectin3 showed that both the 67- and the 120-kDa proteins carry galectin3 epitopes whereas the 37LRP does not. These data suggest that the 67LR is a heterodimer stabilized by strong intramolecular hydrophobic interactions, carried by fatty acids bound to the 37LRP and to a galectin3 cross-reacting molecule. [less ▲]

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See detailVisual evoked potentials during long periods of pattern-reversal stimulation in migraine.
Afra, J; Cecchini, A P; DE PASQUA, Victor ULg et al

in Brain : A Journal of Neurology (1998), 121(2), 233-41

We have previously shown that during repetitive pattern-reversal stimulation, lasting 2 min, the amplitude of the visual evoked potential (PR-VEP) increases in migraineurs when tested interictally whereas ... [more ▼]

We have previously shown that during repetitive pattern-reversal stimulation, lasting 2 min, the amplitude of the visual evoked potential (PR-VEP) increases in migraineurs when tested interictally whereas it decreases in healthy control subjects. According to Sappey-Marinier et al. (J Cereb Blood Flow Metab 1992; 12: 584-92) habituation of the PR-VEP in normal subjects is maxima after 12 min, at a time when there is a decrease of stimulation-enhanced lactate levels in the occipital cortex. We have therefore compared PR-VEP during long periods of repetitive stimulation in healthy control subjects (n = 25) and in patients suffering from migraine without (n = 25) and with aura (n = 15) between attacks. During uninterrupted stimulation at 3.1 Hz VEPs were sequentially averaged in blocks of 100 responses for a total duration of 15 min and analysed in terms of latencies and peak-to-peak amplitudes of N1-P1 and P1-N2 peaks. Amplitude changes from the baseline were calculated for each block, by comparison with the first block, and analysed statistically using Zerbe's method. The N1-P1 and P1-N2 amplitudes in the first block tended to be lower in migraineurs than in healthy control subjects. During the 15 min of stimulation, amplitudes of both components progressively decreased in control subjects, but remained stable in both groups of patients. The difference between patients and control subjects proved to be significant (P < 0.05). The neurophysiological data were not correlated with clinical features such as attack frequency or duration of illness. These results are yet another demonstration in migraine of an interictal habituation deficit in cortical information processing, which might favour lactate accumulation in sensory cortices during sustained activation. [less ▲]

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See detail1-anilino-8-naphtalene sulfonate probes a gastric HK-ATPase potassium site whose access requires ionophores.
Festy, F.; Lins, Laurence ULg; Gallet, X. et al

in Journal of Membrane Biology (1998), 165(2), 153-60

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we ... [more ▼]

1-anilino-8-naphtalenesulfonate (ANS) is a hydrophobic dipole previously used to demonstrate that the proton for potassium exchange by the gastric HK-ATPase is electroneutral. In this paper, we demonstrate that ANS binds to gastric membranes and probes conformational changes of the HK-ATPase independently of any active H for K exchange. Conformational changes require the presence of potassium-valinomycin and are not triggered by sodium. Potassium effect is enhanced by ATP, in the presence and in the absence of magnesium and, by ADP, in the presence of magnesium. Labeling of the pig HK-ATPase K518 by fluorescein-5-isothiocyanate inhibits the enzyme activity and knocks out the ATP effect on ANS fluorescence. Scherring 28080 and the monoclonal antibody 95-111, two competitive inhibitors of K-activated ATPase dephosphorylation, do not modify K-effect on ANS fluorescence but inhibit ATP effects. This supports that ANS does not probe K-site between the H1-H2 loop. Treatment of gastric membranes with trypsin does not inhibit the ANS response to potassium but does inhibit the response to ATP. This suggests that the ATP site inducing the ANS response is cytoplasmic and the potassium site is intramembranous. Titration reveals that one mole of ANS interacts with one mole of ATPase. We suggest that ANS probes a hydrophobic potassium site of gastric ATPase and that addition of ATP and ADP-Mg embed that site. [less ▲]

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See detailnote sous J.P. Etalle, 3 février 1997
Moreau, Pierre ULg

in Revue de Droit Judiciaire et de la Preuve = Tijdschrift voor Procesrecht en Bewijsrecht (1998), (5), 312-313

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See detailNote sur un sceau-cylindre et une bulla provenant de Horum Höyük
Tunca, Önhan ULg

in Anatolia Antiqua 7 (1998)

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