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See detailStabilization of T7-Promoter-Based Parhs Expression Vectors Using the Parb Locus
De Moerlooze, L.; Struman, Ingrid ULg; Renard, Andre et al

in Gene (1992), 119(1), 91-3

We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct ... [more ▼]

We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell generations, even in the absence of selection by an antibiotic present in the culture media, both with or without IPTG induction. [less ▲]

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See detailSequence of the Subtilisin-Encoding Gene from an Antarctic Psychrotroph Bacillus Ta41
Davail, S.; Feller, Georges ULg; Narinx, E. et al

in Gene (1992), 119(1), 143-4

The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph, Bacillus TA41, was determined. The primary structure of the subtilisin precursor corresponds to a preproenzyme of ... [more ▼]

The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph, Bacillus TA41, was determined. The primary structure of the subtilisin precursor corresponds to a preproenzyme of 419 amino acids. Asp144, His181 and Ser359 are the proposed catalytic residues of the protease active site. [less ▲]

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See detailUltraviolet imaging of the Jovian aurora with the Hubble Space Telescope
Dols, V.; Gérard, Jean-Claude ULg; Paresce, F. et al

in Geophysical Research Letters (1992), 19

We present here for the first time a Lyman-alpha image of the north polar region of Jupiter obtained with the Faint Object Camera on board the Hubble Space Telescope a few hours after the encounter of the ... [more ▼]

We present here for the first time a Lyman-alpha image of the north polar region of Jupiter obtained with the Faint Object Camera on board the Hubble Space Telescope a few hours after the encounter of the ULYSSES spacecraft with Jupiter. The presence of high latitude regions of enhanced emission is clearly observed. A comparison with the location of the 'UVS oval', the Io (L = 6) and high-latitude field-line footprints shows that the best agreement is obtained with the L not less than 15 footprint and the UVS oval which are close to each other for the particular longitudinal sector observed. These two L-shells correspond to two possible sources of precipitation: particles originating respectively from the region of the plasma torus of Io in a distorted magnetic field or particles from the distant magnetosphere by analogy with the terrestrial aurora. [less ▲]

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See detailThe outstanding photometric variations of the Ap star HD 7676 = VV Scl
Renson, Pierre ULg; Manfroid, Jean ULg

in Astronomy and Astrophysics (1992), 263

Contrary to what was formerly believed, VV Scl is not an eclipsing star but an Alpha CVn type variable with period P = 5.0976 +/- 0.0001 d. The range of the luminosity variation is remarkably large for an ... [more ▼]

Contrary to what was formerly believed, VV Scl is not an eclipsing star but an Alpha CVn type variable with period P = 5.0976 +/- 0.0001 d. The range of the luminosity variation is remarkably large for an Ap star, especially in B of Johnson's system, and in b and mostly in v of Stromgren's one. A secondary minimum appears near the maximum in B, b, an v. The main minima are in phase in all colors. [less ▲]

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See detailEnseigner l'holocauste
Jadoulle, Jean-Louis ULg

in Bulletin de Clio (1992), n°94

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See detailPresurgical octreotide treatment in acromegaly.
Stevenaert, Achille ULg; Harris, A. G.; Kovacs, K. et al

in Metabolism : Clinical and Experimental (1992), 41(9 Suppl. 2), 51-8

The aim of this study was to determine the role of octreotide administration in acromegalic patients as a preparation for selective adenomectomy using transsphenoidal route. Octreotide was administered ... [more ▼]

The aim of this study was to determine the role of octreotide administration in acromegalic patients as a preparation for selective adenomectomy using transsphenoidal route. Octreotide was administered for 3 to 6 weeks before surgery in 12 patients and for 4 to 39 months in 25 patients. The clinical response was judged as excellent or good in 10 of 12 patients from group I and in 23 of 25 patients from group II. Marked reduction (ie, greater than 50% of initial values) in serum growth hormone (GH) levels was seen in all patients, with levels to less than 5 micrograms/L in 68% of patients and less than 2 micrograms/L in 27%. Insulin-like growth factor 1 (IGF-1) levels decreased to within normal limits in half the cases. During long-term treatment, an escape phenomenon could be seen. Varying degrees of tumor shrinkage were seen in more than 50% of cases. During surgery, with regard to the relative ease or difficulty in removing the tumor, the consistency of the tumor and the separation of normal from pathological tissue, no significant difference was observed between patients given octreotide and those from control series. Morphological changes in adenomatous tissue were rather small. The surgical outcome was similar in the pretreated series as in the control series, except in enclosed adenomas, which showed a tendency to a higher success rate. Since octreotide improves both the clinical condition and hormonal parameters and induces varying degrees of tumor shrinkage, it is potentially useful as an adjunct to surgery. Morphological data suggest that octreotide exercises a functional inhibitory effect on GH release. [less ▲]

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See detailThe Human Placenta Becomes Haemochorial at the 13th Week of Pregnancy
Foidart, Jean-Michel ULg; Hustin, Jean-Michel; Dubois, Michel ULg et al

in International Journal of Developmental Biology (1992), 36(3), 451-3

Histological specimens of recent implantation sites are the basis of our current concept on human embryo implantation and placental development. In the Carnegie Collection maternal red blood cells were ... [more ▼]

Histological specimens of recent implantation sites are the basis of our current concept on human embryo implantation and placental development. In the Carnegie Collection maternal red blood cells were detected early in the primitive intervillous space (10th-12th day after conception). These cells were localized to the trophoblastic lacunae and originated from distended peripheral maternal sinusoids (Kaufmann, 1981). The classical theory states that progressively more and more maternal vessels are tapped. A true maternal blood flow is established around the 29th day. Dynamic investigations of human placental development in vivo are scarce and hampered by ethical considerations and the absolute requirement to refrain from using non aggressive and potentially harmful techniques. Despite these limitations such studies provide new insights that surprisingly contradict our previously and seemingly definitely established knowledge of the early phases of placental vascularization, and lead us to conclude that there is an absence of maternal blood circulation in the intervillous placental space (IVS) during the 12 first weeks of human pregnancy. [less ▲]

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See detailEffect of estradiol supplementation on superovulation in swamp buffalo
Uoc, N. T.; Nguyen, B. X.; Ty, L. V. et al

in Theriogenology (1992), 38(3), 471-478

The effect of estradiol-17β (E2) supplementation on superovulation with (PMSG) or (FSH) was investigated in Swamp buffalo. Sixty-eight buffalo were treated in seven groups. Group 1 served as control and ... [more ▼]

The effect of estradiol-17β (E2) supplementation on superovulation with (PMSG) or (FSH) was investigated in Swamp buffalo. Sixty-eight buffalo were treated in seven groups. Group 1 served as control and was superovulated by standard PMSG or FSH treatment used in routine bovine embryo transfer protocols. Group 2 was superovulated by standard PMSG regimen plus two injections of E2 at a 48 h interval beginning one day before the onset of gonadotropin treatment (short-term supplementation) for a total dosage of 2.5 mg E2; Groups 3 and 4 received the same regimen as Group 2, but in doses of 5.0 and 7.5 mg E2, respectively. Group 5 received the standard FSH regimen (40% LH). Group 6 received short-term E2 (7.5 mg) supplementation of FSH-p. Group 7 was superovulated by standard FSH regimen (40% LH) plus three injections of E2 at 48–72 h intervals beginning five days before the onset of gonadotropin treatment (long-term supplementation) for a total dosage of 7.5 mg E2. The number of corpora lutea (CL) and follicles ≥ 8 mm in diameter were recorded by palpation per rectum and after slaughter. The mean numbers of CL and follicles were 0.99, 5.8, 8.0, 10.6, 4.0, 3.9, 8.1 and 0.25, 6.8, 6.2, 6.2, 1.6, 0.0, 4.1 for Groups 1, 2, 3, 4, 5, 6, 7, respectively. In Group 7, the rates of nonsurgical and postmortem embryo recovery were 46 and 90.4%, respectively and 54.4% of the collected ova were fertilized. These results indicate the possibility of producing viable embryos in buffalo by using E2 supplementation for the gonadotropin treatment. [less ▲]

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See detailSinglet Oxygen Quantum Yield of Sulfur and Selenium Analogs of Psoralen
Seret, Alain ULg; Piette, Jacques ULg; Jakobs, Andréas et al

in Photochemistry and Photobiology (1992), 56(3), 409-412

The quantum yield of singlet oxygen production by eight newly synthesized sulfur and selenium analogs of psoralen irradiated with UV-A (366 nm) has been determined in CCl4 with the help of the steady ... [more ▼]

The quantum yield of singlet oxygen production by eight newly synthesized sulfur and selenium analogs of psoralen irradiated with UV-A (366 nm) has been determined in CCl4 with the help of the steady state luminescence technique. The new psoralen derivatives are generally better singlet oxygen producers than psoralen itself. In particular, the replacement of selenophene for furan and/or of thiopyrone for pyrone induces an important enhancement of the singlet oxygen quantum yield. [less ▲]

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See detailTransdiaphragmatic Pressure Measurement in Cattle: Technical Data
Desmecht, Daniel ULg; Rollin, Frédéric ULg; Lekeux, Pierre ULg

in Research in Veterinary Science (1992), 53(2), 148-53

Techniques and methods required for accurate and reproducible measurements of transdiaphragmatic pressure (Pdi), were studied in seven-week and five-month-old calves. Since reliable pleural pressure ... [more ▼]

Techniques and methods required for accurate and reproducible measurements of transdiaphragmatic pressure (Pdi), were studied in seven-week and five-month-old calves. Since reliable pleural pressure measurement in cattle has already been described, separate experiments were conducted to compare retrodiaphragmatic pressures measured (i) with balloon-catheters and needle catheters, (ii) before and after eructation and (iii) in the ruminal and in the peritoneal cavities. Whereas respiratory-induced pressure changes were equally transmitted throughout the rumen content, a marked anteroposterior damping was observed as the recording site in the abdominal cavity was moved away from the diaphragm (P < 0.0001). It was concluded that the most appropriate and accurate way to measure Pdi changes in polygastric animals is to measure the pressure changes existing in the mid-thoracic portion of the oesophagus and in the liquid content of the rumen with balloon-tubing units. Neither growth- and diet-related rumen enlargement nor eructation, which are two typical characteristics of ruminants, altered the reliability of retrodiaphragmatic pressure measurements in the two age groups studied. [less ▲]

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See detailDetection of the Photoactive Protochlorophyllide-Protein Complex in the Light During the Greening of Barley
Franck, Fabrice ULg; Strzalka, K.

in FEBS Letters (1992), 309(1), 73-7

A photoactive protochlorophyllide-protein complex with absorbance and fluorescence maxima at 648 and 653 nm was detected in greening barley leaves without any re-darkening. The variations of the ... [more ▼]

A photoactive protochlorophyllide-protein complex with absorbance and fluorescence maxima at 648 and 653 nm was detected in greening barley leaves without any re-darkening. The variations of the amplitudes of the absorbance and the fluorescence of the photoactive protochlorophyllide with greening time at two different light intensities indicate a close relationship between the rate of chlorophyll synthesis and the amount of the complex during the first hours. The chlorophyllide resulting from photoreduction during greening has an absorbance maximum at 684 nm, which shifts towards a shorter wavelength within a few seconds, indicating rapid liberation of the pigment from the enzyme. We conclude that chlorophyll accumulation proceeds through continuous regeneration and phototransformation of the photoactive complex. [less ▲]

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See detailMacromolecular engineering of polylactones and polylactides. 9. Synthesis, characterization, and applications of omega-primary amine poly(ε-caprolactone)
Degée, Philippe; Dubois, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1992), 25

The synthesis and use of functional diethylaluminum alkoxides (Et2AlORX) as initiators for the ring-opening polymerization of ε-caprolactone have been investigated as a possible strategy to prepare omega ... [more ▼]

The synthesis and use of functional diethylaluminum alkoxides (Et2AlORX) as initiators for the ring-opening polymerization of ε-caprolactone have been investigated as a possible strategy to prepare omega-primary amino poly(ε-caprolactone) (PCL). In a first approach, an initiator containing ethylphthalimide as the functional-RX group has been synthesized. That protected amino group has been successfully attached at the end of PCL chains. The conversion of the phthalimide end group into a primary amine has however failed, whatever the experimental conditions. In an alternative approach, an alkyl bromide has been considered as the functional group to be attached to PCL chains, via the appropriate initiator. The end functionalization was quantitative and the bromo end group converted into an azido group which was subsequently reduced to the expected primary amine. Omega-primary amino PCL has proved to be an effective macroinitiator for the ring-opening polymerization of γ-benzylglutamate N-carboxy anhydride with the formation of a poly(caprolactone-b-peptide) diblock copolymer. [less ▲]

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See detailEvaluation of the Efficacy and Safety of Oral Tiludronate in Paget's Disease of Bone. A Double-Blind, Multiple-Dosage, Placebo-Controlled Study
Reginster, Jean-Yves ULg; Colson, F.; Morlock, G. et al

in Arthritis & Rheumatism : Arthritis Care & Research (1992), 35(8), 967-74

OBJECTIVE. To assess the optimal dosage of oral tiludronate in Paget's disease of bone. METHODS. We studied 149 patients with Paget's disease, in a double-blind, randomized, placebo-controlled trial ... [more ▼]

OBJECTIVE. To assess the optimal dosage of oral tiludronate in Paget's disease of bone. METHODS. We studied 149 patients with Paget's disease, in a double-blind, randomized, placebo-controlled trial. Patients were randomly assigned to 1 of 5 therapeutic groups: a daily dose of 100 mg, 200 mg, 400 mg, or 800 mg of oral tiludronate, or a placebo. Treatment was for 3 months, followed by 3 months of placebo-controlled followup. Serum alkaline phosphatase activity (SAP) and fasting urinary excretion of hydroxyproline/creatinine (OH/Cr) were measured monthly, as were biochemical parameters reflecting renal, hepatic, and hematologic functions. Analgesic efficacy was self-evaluated from a visual analog scale and a global pain index. RESULTS. Statistical analysis revealed that beginning at a dosage of 200 mg/day, there was a direct dose-dependent effect on the reduction of SAP and OH/Cr levels. Reduction of SAP levels was clinically significant at a dosage of 400 mg (44.9 +/- 4.2% reduction at 90 days and 49.2 +/- 4.5% at 180 days, mean +/- SEM) and at 800 mg (53.4 +/- 5% at 90 days and 59.3 +/- 4.6% at 180 days). There was a significant reduction in pain in all groups, including the group taking placebo. In only those taking 800 mg/day of tiludronate was there a significant frequency of complete resolution of pain (versus placebo). Aside from mild gastrointestinal disturbances, as experienced with other oral bisphosphonates, clinical tolerance of all 5 regimens was good. Exhaustive biochemical investigations failed to reveal significant toxicity of tiludronate up to the 800-mg daily dose investigated. CONCLUSION. Because of its significantly better antiresorptive effects and greater analgesic properties (compared with lower dosages), combined with the excellent clinical and biochemical tolerance, the 800-mg daily dose of tiludronate appears to be optimal for the treatment of Paget's disease of bone. [less ▲]

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See detailAldosterone-secreting adrenal adenoma as part of multiple endocrine neoplasia type 1 (MEN1): loss of heterozygosity for polymorphic chromosome 11 deoxyribonucleic acid markers, including the MEN1 locus.
Beckers, Albert ULg; Abs, R.; van der Auwera, B. et al

in Journal of Clinical Endocrinology and Metabolism (1992), 75(2), 564-70

A 63-year-old female presented with the extremely rare occurrence of an aldosterone-secreting adrenocortical adenoma as part of the syndrome of multiple endocrine neoplasia type 1 (MEN1). Only two other ... [more ▼]

A 63-year-old female presented with the extremely rare occurrence of an aldosterone-secreting adrenocortical adenoma as part of the syndrome of multiple endocrine neoplasia type 1 (MEN1). Only two other MEN1 patients were reported in the literature with hyperaldosteronism. The patient's MEN1 syndrome consisted of the association of primary hyperparathyroidism due to parathyroid adenoma, a prolactinoma, and a toxic multinodular goiter. Elevated basal and meal-stimulated serum PP levels without demonstrable pancreatic tumor were also found. Genetic analysis of the aldosterone-secreting adenoma with DNA markers localized on chromosome 11 showed loss of heterozygosity in tumor DNA. Since the MEN1 syndrome is caused by loss of the tumor suppressor gene on chromosome 11 in the 11q13 region, it is probable that the same mechanism is associated with the formation of the adrenocortical adenoma. [less ▲]

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See detailRadiosensitizing and repair-inhibiting properties of dipyridamole.
COUCKE, Philippe ULg; Rutz, H-P; Calmes, J-M et al

in Radiotherapy & Oncology (1992), 24(24), 246-250

Radioresistance and postirradiation repair of potentially lethal damage (PLD repair) are important factors underlying failure to control local disease in cancer. Dipyridamole (DP) is known as a modifier ... [more ▼]

Radioresistance and postirradiation repair of potentially lethal damage (PLD repair) are important factors underlying failure to control local disease in cancer. Dipyridamole (DP) is known as a modifier of the action of cytotoxic drugs. We therefore investigated DP as a potential radiosensitizer and inhibitor of PLD repair in X-irradiated Chinese hamster ovary (CHO) cells in vitro. Exposure to the drug alone resulted in a slight reduction of the clonogenic capacity of the cells. Preincubation for 18 h with 10 and 20 microM DP in cells subcultured at low density, led to a significant radiosensitization. In confluent density-inhibited cultures, preincubation alone as well as pre- and postincubation with 20 microM DP resulted in a significant inhibition of PLD repair. Dipyridamole and related compounds may thus be useful tools for modifying and investigating the response of mammalian cells to radiation. [less ▲]

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See detailLe trou d'ozone et son impact sur la chronobiologie. Sommes-nous directement concernés?
Nikkels, Arjen ULg; Gerardy-Goffin, F.; Pierard-Franchimont, Claudine ULg et al

in Revue Médicale de Liège (1992), 47(8), 415-418

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See detailFlow cytometric method for the detection of gpI antigens of varicella zoster virus and evaluation of anti-VZV agents
Snoeck, R.; Schols, D.; Sadzot-Delvaux, Catherine ULg et al

in Journal of Virological Methods (1992), 38(2), 243-254

Varicella zoster virus (VZV) is responsible for a primary infection (varicella.) and, upon reactivation, zoster, which in immunocompromised patients, may both lead to life-threatening disseminated disease ... [more ▼]

Varicella zoster virus (VZV) is responsible for a primary infection (varicella.) and, upon reactivation, zoster, which in immunocompromised patients, may both lead to life-threatening disseminated disease. There is a great need for antiviral compounds that are effective inhibitors of VZV replication and for rapid and accurate methods for evaluating viral sensitivity to candidate anti-VZV drugs. With the monoclonal antibody (mAb) (VL8), which is directed against the gpI of VZV, and using the fluorescence-activated cell sorter (FACS) we could readily demonstrate expression of the VZV gpI antigen at 3-4 days after VZV infection. (E)-5-(2-Bromovinyl)-2'-deoxyuridine (BVDU), (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and (S)-1-(3-hydro-xy-2-phosphonylmethoxypropyl)cytosine (HPMC) were shown to be potent inhibitors of VZV replication by this assay. HPMPA and HPMPC were also active against thymidine kinase-deficient (TK-) VZV whereas BVDU was not. The flow cytometric method based on the use of mAb VL8 may be of considerable help for the early diagnosis of VZV infection and evaluation of viral sensitivity to antiviral drugs. [less ▲]

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See detailExperimental Acute Traumatic Injury of the Adult Rat Spinal Cord by a Subdural Inflatable Balloon: Methodology, Behavioral Analysis, and Histopathology
Martin, Didier ULg; Schoenen, Jean ULg; Delree, P. et al

in Journal of Neuroscience Research (1992), 32(4), 539-50

We describe an experimental model to produce closed traumatic injuries to the spinal cord of adult rats. This model uses an inflatable balloon that is introduced in the dorsal subdural space and moved to ... [more ▼]

We describe an experimental model to produce closed traumatic injuries to the spinal cord of adult rats. This model uses an inflatable balloon that is introduced in the dorsal subdural space and moved to a location rostral to the laminectomy site. The spinal cord trauma can be graded by varying either the duration of compression or the volume of saline used to inflate the balloon. The locomotor deficit of animals with various degrees of injury has been assessed at increasing delays after trauma. The parameters generating transient or definitive deficits of varying intensity were defined. Some injured animals underwent nuclear magnetic resonance imaging. Detailed histopathological studies demonstrated that the extent of the spinal lesion was significantly correlated with the physical parameters of compression and with the severity of the behavioral deficit. [less ▲]

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See detailImpairment of renal function with increasing blood lead concentrations in the general population
Staessen, Jan; Lauwerys, Robert; Buchet, Jean-Pierre et al

in New England Journal of Medicine [=NEJM] (1992), 327(3), 151-156

Cadmium and lead are toxic to the kidney. Both metals are known to induce nephropathy in subjects with heavy exposure. Environmental exposure to cadmium is associated with renal tubular dysfunction, but ... [more ▼]

Cadmium and lead are toxic to the kidney. Both metals are known to induce nephropathy in subjects with heavy exposure. Environmental exposure to cadmium is associated with renal tubular dysfunction, but few studies have attempted to evaluate the renal effects of environmental lead exposure. A weak positive correlation between serum creatinine and blood lead concentrations was found in men, but not women, in the British civil service. We investigated the relation between lead exposure and renal function in the general population, using data obtained during the Cadmibel (Cadmium in Belgium) Study. Lead exposure was estimated by measuring blood concentrations of lead and zinc protoporphyrin, which is increased in the presence of high lead levels. [less ▲]

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See detailLiving (co)polymerization of adipic anhydride and selective end functionalization of the parent polymer
Ropson, Nathalie; Dubois, Philippe; Jérôme, Robert ULg et al

in Macromolecules (1992), 25(14), 3820-3824

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See detailErrata au Bulletin d'Information 40
Renson, Pierre ULg

in Bulletin d'Information du Centre de Données Stellaires (1992), 41

Not Available

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See detailSite-Directed Mutagenesis of the Streptomyces R61 Dd-Peptidase. Catalytic Function of the Conserved Residues around the Active Site and a Comparison with Class-a and Class-C Beta-Lactamases
Hadonou, Ayaovi Medard; Wilkin, Jean-Marc; Varetto, Louis ULg et al

in European Journal of Biochemistry (1992), 207(1), 97-102

The importance of various residues in the Streptomyces R61 penicillin-sensitive DD-peptidase has been assessed by site-directed mutagenesis. The replacement of the active Ser62 by a Cys residue yielded an ... [more ▼]

The importance of various residues in the Streptomyces R61 penicillin-sensitive DD-peptidase has been assessed by site-directed mutagenesis. The replacement of the active Ser62 by a Cys residue yielded an inactive protein which was also unable to recognize penicillin. The activity of the Lys65----Arg mutant with the peptide and thiolester substrates was decreased 100-200-fold and the rate of penicillin inactivation was decreased 20,000-fold or more. The mutant thus behaved as a poor, but penicillin-resistant, DD-peptidase. The other studied mutations, the mutations Phe58----Leu, Tyr90----Asn, Thr101----Asn, Phe164----Ala, Asp225----Glu and Asp225----Asn had little influence on the catalytic and penicillin-binding properties. The Asp225 mutants did not exhibit an increased sensitivity to cefotaxime. The Phe164----Ala mutant was significantly more unstable than the wild-type enzyme. [less ▲]

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See detailPhysical constraints on color transformations
Manfroid, Jean ULg

in Astronomy and Astrophysics (1992), 260

Color-transformation procedures in photometry should ideally obey physical principles. A few of these rules, of particular interest in the study of reddened stars, of multiple stars, stellar clusters and ... [more ▼]

Color-transformation procedures in photometry should ideally obey physical principles. A few of these rules, of particular interest in the study of reddened stars, of multiple stars, stellar clusters and galaxies are discussed. Directions to minimize the transformation errors for these objects are given. [less ▲]

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See detailSolar system observations with HST
Paresce, Francesco; Gérard, Jean-Claude ULg

in Environment Observation and Climate Modelling Through International Space Projects (1992)

Imaging and spectrographic measurements of solar system objects with the Hubble Space Telescope (HST) are reviewed from a scientific and technical standpoint. Special emphasis is placed on observations of ... [more ▼]

Imaging and spectrographic measurements of solar system objects with the Hubble Space Telescope (HST) are reviewed from a scientific and technical standpoint. Special emphasis is placed on observations of Jupiter and Io during the Ulysses encounter by the Faint Object Camera and HRS. The bottom line is that, although the mirror aberration limits HST's extragalactic performance, the potential for a deep systematic probe of planetary conditions over a wide range of observing conditions is still practically intact and will get better as the bugs are ironed out and the COSTAR (Corrective Optics Space Telescope Axial Replacement) fix is implemented. [less ▲]

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See detailErrata au Bulletin d'Information 40
Renson, Pierre ULg

in Bulletin d'Information du Centre de Données Stellaires (1992), 41

Not Available

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See detailPressure-Flow Relationships of the Pulmonary Circulation During Endotoxin Infusion in Intact Dogs
D'Orio, Vincenzo ULg; Fatemi, M.; Marnette, J. M. et al

in Critical Care Medicine (1992), 20(7), 1005-13

BACKGROUND AND METHODS: We aimed to characterize the effects of an endotoxin insult (Escherichia coli 0127:B8) on the relationships between pulmonary vascular pressure and flow in intact dogs. To achieve ... [more ▼]

BACKGROUND AND METHODS: We aimed to characterize the effects of an endotoxin insult (Escherichia coli 0127:B8) on the relationships between pulmonary vascular pressure and flow in intact dogs. To achieve this goal, multipoint plots of total pressure gradient, arterial pressure gradient, and venous pressure gradient vs. flow were generated by graded inflation of a right atrial balloon, which was used to vary flow. The partitioning of the total pressure decrease across the pulmonary vasculature (total pressure gradient = pulmonary arterial pressure-pulmonary artery occlusion pressure [PAOP]) into gradients across pulmonary arterial (arterial pressure gradient = pulmonary arterial pressure--effective capillary pressure) and pulmonary venous (venous pressure gradient = effective capillary pressure--PAOP) regions was assessed by a waveform mathematical analysis of the pulmonary arterial pressure profile during arterial occlusion, with computation of both PAOP and effective pulmonary capillary pressures. Slopes and extrapolated pressure intercepts from linear regression fits to the pulmonary vascular pressure/flow plots were determined in seven dogs after a 2-hr endotoxic infusion interval and were compared with the corresponding values that characterized a similar group of sham-operated dogs. RESULTS: Under normal conditions, the extrapolated pressure intercept for pulmonary arterial pressure gradient was virtually 0 mm Hg; for total pulmonary arterial pressure gradient and pulmonary venous pressure gradient, the mean extrapolated pressure intercepts were substantially positive: 2.4 +/- 0.2 and 2.1 +/- 0.3 mm Hg, respectively. Endotoxin infusion at 0.25 micrograms/kg/min significantly increased the pressure intercepts from 2.4 to 8.7 and from 2.1 to 8.3 mm Hg of total pressure gradient and venous pressure gradient vs. flow, respectively. This infusion produced a minor, nonsignificant change in the intercept of arterial pressure gradient vs. flow, whereas it increased its slope significantly (p less than .05) from 0.036 to 0.081 mm Hg/mL/min/kg. CONCLUSIONS: These data suggest that endotoxin's effects on vascular resistance are exerted at two different loci such that these effects are additive. These endotoxin-induced effects consisted of increased vascular resistance of the arterial segment and appearance of a Starling resistor at the venous side of the pulmonary circulation, which acted as the relevant back-pressure to flow. [less ▲]

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See detailLow sodium diet in essential hypertension- Effect on blood cell ions and hemodynamic parameters
Krzesinski, Jean-Marie ULg; Du, Fenghe; Pequeux, Marie-Louise et al

in American Journal of Hypertension : Journal of the American Society of Hypertension (1992), 5

The influence of salt restriction for 3 months on blood pressure, peripheral vascular resistance (observed by occlusive plethysmography), erythrocyte sodium, platelet calcium, and pH, was studied in eight ... [more ▼]

The influence of salt restriction for 3 months on blood pressure, peripheral vascular resistance (observed by occlusive plethysmography), erythrocyte sodium, platelet calcium, and pH, was studied in eight untreated essential hypertensive patients. A low salt diet decreases blood pressure, vascular resistances, erythrocyte sodium, and platelet calcium, but not platelet pH. A strong positive correlation was noted between baseline platelet calcium and vascular resistances (r=0.95, P < .01). But during the salt restriction period, a negative correlation has been observed between the changes in these parameters, which casts doubt on the use of the platelet as a mode of the smooth muscle cell. [less ▲]

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See detailInternal mammary artery bypass graft for impaired coronary perfusion after neonatal arterial switch operation
Grabitz, R. G.; Messmer, B. J.; SEGHAYE, Marie-Christine ULg et al

in European Journal of Cardio - Thoracic Surgery (1992), 6(7), 388-90

Myocardial ischaemia caused by perfusion impairment of translocated coronary arteries is the major cause of perioperative mortality after neonatal arterial switch operation for transposition of the great ... [more ▼]

Myocardial ischaemia caused by perfusion impairment of translocated coronary arteries is the major cause of perioperative mortality after neonatal arterial switch operation for transposition of the great arteries. We report the successful use of the right internal mammary artery as a bypass graft to a dominant right coronary artery to treat insufficient perfusion of this artery in a newborn. Eight months later, coronary angiography showed a full blood supply of the right coronary artery across the internal mammary anastomosis. After a follow-up period of more than 30 months, somatic development, electrocardiogram and echocardiographically determined contractility of both ventricles are practically normal indicating regular function of the bypass graft. [less ▲]

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See detailUn nouvel atout dans la lutte antifongique : Bacillus subtilis.
Vanhentenryck, F.; Jacques, Ph.; Thonart, Philippe ULg

in Bulletin de liaison FAO (1992), 4

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See detailRegional Brain Glucose Metabolism in Patients with Complex Partial Seizures Investigated by Intracranial Eeg
Sadzot, Bernard ULg; Debets, R. M.; Maquet, Pierre ULg et al

in Epilepsy Research (1992), 12(2), 121-9

We performed interictal 18F-2-fluoro-2-deoxy-D-glucose positron emission tomography (18FDG-PET) studies in 57 patients with complex partial epilepsy (CPE), not controlled by medical treatment and ... [more ▼]

We performed interictal 18F-2-fluoro-2-deoxy-D-glucose positron emission tomography (18FDG-PET) studies in 57 patients with complex partial epilepsy (CPE), not controlled by medical treatment and considered for surgical resection of their epileptic focus. A precise localization of the epileptic focus was obtained in 37 of these patients with a combination of subdural and depth electrodes. We visually inspected the metabolic images; we also measured glucose consumption in a number of brain regions and compared the values with those obtained in 17 normal controls. Eighty-two percent of the 57 patients had an area of glucose hypometabolism on the 18FDG-PET images. Six patients had a frontal epileptic focus, 3 of them had a frontal lobe hypometabolism. Twenty-six patients had a unilateral temporal lobe focus and all of them displayed a temporal lobe hypometabolism. The asymmetry was more pronounced in the lateral temporal cortex (-20%) than in the mesial part of the temporal lobe (-9.6%). In each cortical brain region on the side of the epileptic focus (except the sensorimotor cortex), glucose consumption rate was lower than in the contralateral region or than in controls. No differences could be found between patients with a seizure onset restricted to the hippocampus and patients with a seizure onset involving the hippocampus and the adjacent neocortex. Divergent metabolic patterns were obtained in 5 patients with bilateral temporal seizure foci. Combined with other non invasive techniques (EEG, neuroradiology), PET contributes increasingly to the selection of patients with CPE who could benefit from surgical treatment.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailEffect of Positive End-Expiratory Pressure on Pulmonary Vascular Pressure-Flow Characteristics in Canine Endotoxin Shock
D'Orio, Vincenzo ULg; Fatemi, M.; Mendes, P. et al

in Circulatory Shock (1992), 37(3), 189-97

The vascular pulmonary pressure-flow (P-Q degree) relationships were studied in anesthetized dogs in order to characterize the distribution of total resistance in the pulmonary bed with respect to ... [more ▼]

The vascular pulmonary pressure-flow (P-Q degree) relationships were studied in anesthetized dogs in order to characterize the distribution of total resistance in the pulmonary bed with respect to incremental resistance and critical closure prior to and after endotoxin insult. Incremental resistance was computed as the slope of the P-Q degree relation, whereas critical closure was referred to as the extrapolated pressure intercept at zero flow. P-Q degree coordinates were obtained by varying Q degree through graded inflation of right atrial balloon. The gradients across the arterial segment (Pa = Ppa - Pc) and across the venous segment (Pv = Pc - Pw) of the pulmonary vasculature were defined by the computation of effective capillary pressure (Pc) obtained from the analysis of the transient decay of pulmonary artery pressure (Ppa) toward wedge pressure (Pw) after arterial occlusion. Six group E dogs were infused with endotoxin at a rate of 0.25 microgram/kg min, while six additional animals served as control (group C). Endotoxin induced increases in flow resistance from 0.056 to 0.096 mm Hg/ml/min/kg due to arterial vasoconstriction and increases in critical closure from 2.3 to 8.4 mm Hg due to a venous waterfall. Before and after endotoxin insult, we assessed effects of each of three levels of static lung inflation (PEEP) on P-Q degree relationships.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailEffects of repeated use of progestagen-PMSG treatment for estrus control in dairy goats out of breeding season
Baril, Gérard; Remy, Benoît ULg; Vallet, J. C. et al

in Reproduction in Domestic Animals (1992), 27(3), 161-168

Contents: In order to analyze the effects of repeated use of progestagen-PMSG treatment, estrus and pregnancy results have been analyzed for 1989 in a Saânen dairy goat herd in which breeding takes place ... [more ▼]

Contents: In order to analyze the effects of repeated use of progestagen-PMSG treatment, estrus and pregnancy results have been analyzed for 1989 in a Saânen dairy goat herd in which breeding takes place each year out of season after FGA/PMSG treatment. After the first 1989 treatment (169 goats), percentage of goats showing estrus and kidding have been lower for 59 multiparous than for 46 primiparous and 64 nulliparous females. Moreover, when 38 goats are treated for a second time in 1989, 44.7% exhibited estrus vs 71.0% after the first treatment (P < 0.05). The PMSG binding level before the 1st 1989 treatment is higher for multiparous (17.5 ± 23.1%) than nulli and primiparous (-0.06 ± 0.7 and 1.2 ± 1.9%) and is increased for all parities after treatment (23.2 ± 26.4 after vs 5.7 ± 15.0% before, P < 0.01). For nulliparous and primiparous females; PMSG binding levels are not different for pregnant or not pregnant nulliparous and primiparous goats. On the opposite, PMSG binding rates are higher in non pregnant (25.7 ± 23.3) than in pregnant multiparous goats (6.5 ± 15.9) (P < 0.01). However, when the binding rate is ≤ 5.12% (computerized distributions) multiparous goats exhibit estrus and pregnancy at levels not different from nulliparous or primiparous females (% estrus 95.8 vs 100 or 97.8%, % pregnancy 66.7 vs 70.3 and 63.0% respectively). Repeated use of PMSG during the female life or during one given year leads to active immunization against PMSG (as measured by percentage of binding of PMSG in plasma) decreasing the efficiency of ovarian stimulation out of breeding season. [less ▲]

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See detailDesign Method for Subsonic and Transonic Cascade with Prescribed Mach Number Distribution
Léonard, Olivier ULg; Van den Braembusche, René

in Journal of Turbomachinery (1992), 114(3), 553-560

An iterative procedure for blade design, using a time marching procedure to solve the unsteady Euler equations in the blade-to-blade plane, is presented. A flow solver, which performs the analysis of the ... [more ▼]

An iterative procedure for blade design, using a time marching procedure to solve the unsteady Euler equations in the blade-to-blade plane, is presented. A flow solver, which performs the analysis of the flow field for a given geometry, is transformed into a design method. This is done by replacing the classical slip condition (no normal velocity component) by other boundary conditions, in such a way that the required pressure or Mach number distribution may be imposed directly on the blade. The unknowns are calculated on the blade wall using the so-called compatibility relations. Since the blade shape is not compatible with the required pressure distribution, a nonzero velocity component normal to the blade wall evolves from the new flow calculation. The blade geometry is then modified by resetting the wall parallel to the new flow field, using a transpiration technique, and the procedure is repeated until the calculated pressure distribution has converged to the required one. Examples for both subsonic and transonic flows are presented and show a rapid convergence to the geometry required for the desired Mach number distribution. An important advantage of the present method is the possibility to use the same code for the design and the analysis of a blade. [less ▲]

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See detailInfluence Of Extrusion On Ruminal And Intestinal Disappearance In Sacco Of Pea (Pisum-Sativum) Proteins And Starch
Walhain, Philippe; Foucart, Michel; Thewis, André ULg

in Animal Feed Science & Technology (1992), 38(1), 43-55

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See detailEndogenous Production of Specific Antibodies Does Not Decrease Hypocalcemic Response to Calcitonin in Young Rabbits
Reginster, Jean-Yves ULg; Azria, M.; Lismonde, Stéphanie ULg et al

in Calcified Tissue International (1992), 50

In order to evaluate the potential inhibition of the acute anti-osteoclastic activity of salmon calcitonin (SCT) by specific antibodies (Ab), we compared the SCT-induced hypocalcemic effect in young male ... [more ▼]

In order to evaluate the potential inhibition of the acute anti-osteoclastic activity of salmon calcitonin (SCT) by specific antibodies (Ab), we compared the SCT-induced hypocalcemic effect in young male rabbits with significant titers of high affinity Ab and in matched animals without Ab. Immunization of rabbits was performed by repetitive s.c. injections of SCT and Freund adjuvant. Ab were present in four-fifths of SCT-treated rabbits (Ab+). Their titer varied from 0.8 x 10(-9) to 30 x 10(-9) M/liter and their constant of affinity from 0.97 x 10(9) to 4.2 x 10(9) L/M. Intravenous injection of 1 IU/kg SCT to Ab+ rabbits induced a significant decrease (P less than 0.01) of ionized serum calcium (Ca2+) after 30 minutes (mean +/- SD: -9 +/- 0.6%) and until the 240th minute of the test (-16.7 +/- 4.7%), with a maximum after 120 minutes (-22.6 +/- 2%). This was not significantly different from the hypocalcemic effect measured after the same procedure performed in matched animals without Ab (Ab-): significant decrease in Ca2+ (P less than 0.01) after 30 minutes (-8.2 +/- 2.2%), maximal after 150 minutes (-23.2 +/- 4.9%), and lasting until 210 minutes (-14.5 +/- 3.7%). We conclude that, in the particular model of the male young rabbit, specific anti-SCT Ab do not block or reduce the acute anti-osteoclastic activity of SCT. [less ▲]

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See detailGonadal-Independent Developmental Changes in Activation of N-Methyl-D-Aspartate Receptors Involved in Gonadotropin-Releasing Hormone Secretion
Bourguignon, Jean-Pierre ULg; Gerard, Arlette ULg; Alvarez Gonzalez, Maria-Luz ULg et al

in Neuroendocrinology (1992), 55(6), 634-41

Using hypothalamic explants of male rats, we have shown that the N-methyl-D-aspartate (NMDA) receptors involved in a stimulatory control of gonadotropin-releasing hormone (GnRH) secretion were transiently ... [more ▼]

Using hypothalamic explants of male rats, we have shown that the N-methyl-D-aspartate (NMDA) receptors involved in a stimulatory control of gonadotropin-releasing hormone (GnRH) secretion were transiently activated at 25 days around the time of onset of puberty. This was evidenced by studying the dose-related inhibition of veratridine-induced GnRH secretion by MK-801, a use dependent antagonist of NMDA receptors. An increase in sensitivity of GnRH secretion to the inhibitory effect of MK-801 was used as a marker of increased activation of NMDA receptors involved in stimulation of GnRH secretion. Here, we report on data obtained in intact and castrated rats at different ages. The aim was to determine whether the absence of gonads would affect the developmental changes in activation of NMDA receptors that we described recently. In pubertal (50-day-old) rats, orchidectomy resulted in an activation of NMDA receptors which was nonsignificant after 4 days but significant after 13 days. In prepubertal rats orchidectomized at 5 or 10 days and studied 10 days later, the NMDA receptors involved in GnRH secretion were also more activated than in intact animals. Using explants of intact and castrated animals, a similar increase in activation of NMDA receptors was observed between 15 and 25 days of age, a period preceding onset of puberty. Subsequently, between 25 and 50 days, a reduction in NMDA receptor activation was seen. This decrease was observed in intact rats showing normal sexual development and in castrated rats as well.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailStratigraphie du Dévonien inférieur dans le Massif de la Vesdre (Belgique)
Hance, Luc ULg; Dejonghe, L; Steemans, Philippe ULg

in Annales de la Société Géologique de Belgique (1992), 115(1), 119-134

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See detailBehavioral Demasculinization of Female Quail Is Induced by Estrogens: Studies with the New Aromatase Inhibitor, R76713
Balthazart, Jacques ULg; De Clerck, A.; Foidart, Agnès ULg

in Hormones & Behavior (1992), 26(2), 179-203

The injection before Day 12 of incubation of estradiol benzoate (EB) into Japanese quail eggs produces a complete behavioral demasculinization of adult males that will hatch from these eggs. These males ... [more ▼]

The injection before Day 12 of incubation of estradiol benzoate (EB) into Japanese quail eggs produces a complete behavioral demasculinization of adult males that will hatch from these eggs. These males never show copulatory behavior even after administration of high levels of exogenous testosterone (T). It is usually assumed that such a demasculinization normally takes place in female embryos under the influence of endogenous estrogens but few experimental data are available to confirm the validity of this model. A series of four experiments was performed during which R76713, a triazole derivative that specifically inhibits aromatase (estrogen synthetase) activity, was injected into quail eggs at different stages of incubation to prevent the production of endogenous estrogens. The consequences of these embryonic treatments on the T-activated sexual behavior in adults were then quantified. When injected before Day 12 of incubation, R76713 completely blocked the behavioral demasculinization of females without affecting the behavior of the males. After a treatment with T, almost all R76713-treated females showed as adults a masculine copulatory behavior that was undistinguishable from the behavior of intact males. This effect was fully reversed by the injection in egg of EB demonstrating that the effects of R76713 were specifically due to the suppression of endogenous estrogens. Injection of R76713 during the late phase of the incubation (Day 12 or Day 15) only maintained weak copulatory behavior in females which confirmed that the behavioral demasculinization in quail takes place mainly though not exclusively during the early stages of ontogeny. In a last experiment, we combined an early R76713 treatment with an injection of EB either on Day 9 or on Day 14 of incubation. This showed that the sensitivity to differentiating effects of estrogens varies with age in a sexually differentiated manner. The EB injection on Day 9 demasculinized both male and female embryos. If this injection was delayed until Day 14, it was no longer effective in males but still caused a partial demasculinization of females. This demonstrates that even if females are not yet behaviorally demasculinized on Day 9 of incubation (suppression of aromatase activity at that age will maintain the behavior), their sensitivity to estrogens is already different from that of males. [less ▲]

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See detailJazz & the Blues : The Belgian Connection
Sacré, Robert ULg

in MAWA Review : Quarterly Publication of the Middle Atlantic Writers Association (1992), 7(1), 41-49

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See detailVarietal Distribution Of The Trypsin-Inhibitor Activity In Peas (Pisum-Sativum L)
Leterme, Pascal; Monmart, Thierry; Thewis, André ULg

in Animal Feed Science & Technology (1992), 37(3-4), 309-315

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See detailThe Stimulation of Fibroblasts' Collagen Synthesis by Neoplastic Cells Is Modulated by the Extracellular Matrix
Noël, Agnès ULg; Munaut, Carine ULg; Nusgens, Betty ULg et al

in Matrix (Stuttgart, Germany) (1992), 12(3), 213-20

Human fibroblasts cocultured with neoplastic MCF7 cells produce increased amounts of collagen. A maximal stimulation requires direct cell-cell contacts between tumor cells and fibroblasts. However, this ... [more ▼]

Human fibroblasts cocultured with neoplastic MCF7 cells produce increased amounts of collagen. A maximal stimulation requires direct cell-cell contacts between tumor cells and fibroblasts. However, this effect could be reproduced, although to a lesser extent, by medium conditioned by MCF7 cells, suggesting that it is mediated by a factor produced by MCF7 cells and secreted, at least partly, under a soluble form (Noel et al., 1992). This Collagen Stimulating Factor ("COSF") present in the culture medium displayed a molecular mass between 3,500 to 10,000 daltons, bound to heparin and appeared to be different from the growth factors described until now. The "COSF" can be released from the surface of MCF7 cells by treatment with heparin. The aim of the present work was to investigate the influence of various extracellular matrix components on the production and the release of "COSF". A 3- to 4-fold enhancement of collagen synthesis was observed in coculture on plastic and collagen type I substrates without significant modification of the non-collagen proteins. The increased collagen synthesis was paralleled by an elevation of specific collagen mRNAs level suggesting a regulation at a pretranslational level. On the opposite, in the presence of soluble or insoluble laminin, this stimulation was abolished. Similarly, coculture on "reconstituted basement membrane matrix", matrigel, did not increase collagen production. The "COSF" was found to bind to matrigel and could be released from the basement membrane matrix by treatment with heparin. [less ▲]

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See detailUnilateral Facial Pain as the First Symptom of Lung Cancer: Are There Diagnostic Clues?
Schoenen, Jean ULg; Broux, R.; Moonen, G.

in Cephalalgia : An International Journal of Headache (1992), 12(3), 178-9

We describe three patients with unilateral facial pain due to non-metastatic lung cancer and review 11 published cases. Pain, most frequently located on the right side and around the ear, as well as ... [more ▼]

We describe three patients with unilateral facial pain due to non-metastatic lung cancer and review 11 published cases. Pain, most frequently located on the right side and around the ear, as well as digital clubbing can be clues to an early diagnosis. Compression of the vagus nerve by the tumour or by mediastinal adenopathy is most likely responsible for the facial pain and could play a role in pulmonary osteoarthropathy. [less ▲]

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See detailLes malformations arterio-veineuses congénitales des membres: à propos de 4 cas
Krawczykowski, D.; VAN DAMME, Hendrik ULg; TROTTEUR, Geneviève ULg et al

in Revue Médicale de Liège (1992), 47(6), 284-91

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See detailINFLUENCE OF THE DAILY DISTRIBUTION OF LIGHT ON REPRODUCTION IN THE BARBEL, BARBUS-BARBUS (L)
Poncin, Pascal ULg

in Journal of Fish Biology (1992), 41(6), 993-997

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See detailNucleotide and Derived Amino Acid Sequence of the Subtilisin from the Antarctic Psychrotroph Bacillus Ta39
Narinx, E.; Davail, S.; Feller, Georges ULg et al

in Biochimica et Biophysica Acta (1992), 1131(1), 111-3

The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph Bacillus TA39 was determined. The primary structure of the subtilisin precursor is composed of 420 amino acids ... [more ▼]

The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph Bacillus TA39 was determined. The primary structure of the subtilisin precursor is composed of 420 amino acids giving rise to a mature enzyme of 309 amino acids. Asp-145, His-185 and Ser-361 are the proposed catalytic residues of the active site. [less ▲]

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See detailSystematic color transformation effects in Stromgren photometry
Manfroid, Jean ULg; Sterken, C.

in Astronomy and Astrophysics (1992), 258

A major cause of errors in photometry is to be attributed to the color transformation. A thorough analysis of numerous observing runs carried out in the Stromgren system shows how reliable are the ... [more ▼]

A major cause of errors in photometry is to be attributed to the color transformation. A thorough analysis of numerous observing runs carried out in the Stromgren system shows how reliable are the transformations in the different indices. Conclusions are drawn about the reduction methods, the validity of homogenization procedures, and the choice of the standard stars which define the system. [less ▲]

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See detailThe climate induced variation of the continental biosphere: A model simulation of the last glacial maximum
Friedlingstein, P.; Delire, C.; Müler, J. F. et al

in Geophysical Research Letters (1992), 19

A simplified three-dimensional global climate model was used to simulate the surface temperature and precipitation distributions for the Last Glacial Maximum (LGM), 18000 years ago. These fields were ... [more ▼]

A simplified three-dimensional global climate model was used to simulate the surface temperature and precipitation distributions for the Last Glacial Maximum (LGM), 18000 years ago. These fields were applied to a bioclimatic scheme which parameterizes the distribution of eight vegetation types as a function of biotemperature and annual precipitation. The model predicts a decrease, for LGM compared to present, in forested area balanced by an increase in desert and tundra extent, in agreement with a reconstruction of the distribution of vegetation based on paleodata. However, the estimated biospheric carbon content (phytomass and soil carbon) at LGM is less reduced than in the reconstructed one. Possible reasons for this discrepancy are discussed. [less ▲]

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See detailHD 35155: the First Eclipsing Binary S Star (P=642 Days)
Jorissen, A.; Mayor, M.; Manfroid, Jean ULg et al

in Information Bulletin on Variable Stars (1992), 3730

Not Available

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See detailEffect of Spinal Cord Stimulation on Regional Myocardial Perfusion Assessed by Positron Emission Tomography
de Landsheere, Christian ULg; Mannheimer, C.; Habets, A. et al

in American Journal of Cardiology (1992), 69(14), 1143-9

Spinal cord stimulation (SCS) can relieve symptoms in patients with severe angina pectoris refractory to conventional medical or surgical therapy. This symptomatic improvement may result from decreased ... [more ▼]

Spinal cord stimulation (SCS) can relieve symptoms in patients with severe angina pectoris refractory to conventional medical or surgical therapy. This symptomatic improvement may result from decreased myocardial ischemia. To test this hypothesis, positron emission tomography (PET) and potassium-38 as a flow tracer were used in 8 patients for the quantitative evaluation of regional myocardial perfusion at rest and after exercise, before and during SCS. Potassium uptake was evaluated as myocardial clearance (flow times net extraction) in ml/min/100 g. Tomographic segments were categorized as nonaffected and affected on the basis of the absence or presence of arterial stenosis on coronary angiography and on the basis of thallium scintigraphic data. In nonaffected segments, before SCS, regional myocardial clearance significantly increased from rest (28 +/- 4) to exercise (47 +/- 13 clearance units; p less than 0.004). A similar increase occurred after SCS. In affected segments, before SCS, regional myocardial clearance barely increased (p = 0.065) from rest (26 +/- 6) to exercise (33 less than or equal to 12). In comparison, after SCS, the resting regional myocardial clearance was slightly elevated (29 +/- 8) reflecting an increased double product, but did not increase (p = 0.192) with exercise (34 +/- 12). However, the magnitude and duration of ST-segment depression decreased during treatment with SCS. Anginal pain occurred in all patients during control exercise, but was attenuated in all but one with SCS. These results indicate that SCS improves exercise-induced angina and electrocardiographic signs of ischemia but this influence does not appear to be mediated by changes in regional myocardial perfusion. [less ▲]

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See detailIdentification and Characterization of Glycoprotein Gp1 of Bovine Herpesvirus Type 4
Dubuisson, J.; Pastoret, Paul-Pierre ULg; Thiry, Etienne ULg

in Journal of General Virology (The) (1992), 73((Pt 5)), 1293-6

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had ... [more ▼]

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had been removed by immunoaffinity, a fourth glycoprotein was identified. This protein (gp1) has a high Mr (greater than 300K), is detected about 8 h post-inoculation of infected cells and is strictly expressed as a gamma protein. Moreover, gp1 was identified by a polyclonal antiserum from an infected animal, indicating that this glycoprotein is an antigen recognized by the immune system of infected animals. [less ▲]

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See detailThe Computation of the Cylindrical Structures Applied to Naval Architecture
Rigo, Philippe ULg

in Marine Structures (1992)

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See detailRoll Motion of a Floating Storm surge Barrier
Rigo, Philippe ULg

in Journal of Hydroscience and Hydraulic Engineering (1992)

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See detailOnce-a-day administration of amikacin in neonates: assessment of nephrotoxicity and ototoxicity
Battisti, Oreste ULg; Langhendries, J. P.; Bertrand, J. M. et al

in Developmental Pharmacology and Therapeutics (1992), 20

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See detailContrast behavior between microadenoma and normal pituitary gland after gadolinium injection as a function of time at 1.5 T.
Stadnik, T.; Stevenaert, Achille ULg; Beckers, Albert ULg et al

in Neuroradiology (1992), 34(3), 184-9

The behavior of contrast enhancement between a microadenoma and the normal pituitary gland after gadolinium injection was evaluated in 12 operatively confirmed cases using a repetitive sequence of four ... [more ▼]

The behavior of contrast enhancement between a microadenoma and the normal pituitary gland after gadolinium injection was evaluated in 12 operatively confirmed cases using a repetitive sequence of four coronal T1-weighted spin echo series (T1 SE) (continuous acquisition, TR = 400 ms), followed by conventional coronal T1 SE (TR = 600 ms) and a three-dimensional fast low-angle shot sequence. The first and second acquisitions were useful with respect to delayed scans only in 3 cases (25%). Nevertheless, in these cases confident diagnosis could also be made on pre-contrast studies, which diminishes the real advantage of this finding. For a 1.5 T MRI unit we advocate starting with coronal T1 SE 30 s after a rapid injection of gadolinium. [less ▲]

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See detailThe intervalling effect bias in beta: a note
Corhay, Albert ULg

in Journal of Banking & Finance (1992), 16(1), 61-73

Based on a comprehensive sample of domestic securities traded on the Brussels Stock Exchange, this paper points out the intervalling effect in the estimated betas and examines the speed of convergence of ... [more ▼]

Based on a comprehensive sample of domestic securities traded on the Brussels Stock Exchange, this paper points out the intervalling effect in the estimated betas and examines the speed of convergence of these. The results reveal that the estimated betas seem to converge to their asymptotic values and that their value depends on what day the differencing interval starts. It also appears that the magnitude of the intervalling effect is inversely related to the market value of the firms. [less ▲]

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See detailCalcitonin Metabolism in Senile (Type II) Osteoporosis
Reginster, Jean-Yves ULg; Deroisy, Rita ULg; Bruwier, Maurice ULg et al

in Osteoporosis International : A Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis & the National Osteoporosis Foundation of the USA (1992), 2

The exact role of calcitonin (CT) in the pathogenesis of senile (Type II) osteoporosis remains unknown. Whole plasma calcitonin (iCT) and extracted monomeric calcitonin (eCT) basal levels, metabolic ... [more ▼]

The exact role of calcitonin (CT) in the pathogenesis of senile (Type II) osteoporosis remains unknown. Whole plasma calcitonin (iCT) and extracted monomeric calcitonin (eCT) basal levels, metabolic clearance rate (MCR) and production rate (PR) of iCT and eCT were measured in 41 postmenopausal women, including 14 hip fractures (OP II) and 27 healthy controls. No significant difference appeared for basal iCT levels between OP II (mean +/- SEM: 41.9 +/- 3.4 pg/ml) and controls (mean +/- SEM: 46.2 +/- 5 pg/ml). eCT basal levels were similar in OP II (mean +/- SEM: 5.42 +/- 0.5 pg/ml) and in controls (mean +/- SEM: 7.3 +/- 0.7 pg/ml). MCR were similar in the two groups. iCT PR were similar in OP II (mean +/- SEM: 17.2 +/- 1.5 micrograms/24 h) and controls (mean +/- SEM: 18.6 +/- 1.1 micrograms/24 h). No difference appeared between eCT PR in OP II (mean +/- SEM: 2.3 +/- 0.2 micrograms/24 h) and controls (mean +/- SEM: 3.2 +/- 0.3 pg/ml). From these data, no evidence appears that calcitonin might be one of the determinant factors in the pathogenesis of senile osteoporosis. [less ▲]

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See detailCardiovascular Response to Acute Hypoxia in Double-Muscled Calves
Amory, Hélène ULg; Rollin, Frédéric ULg; Desmecht, Daniel ULg et al

in Research in Veterinary Science (1992), 52(3), 316-24

Hypoxic-induced pulmonary hypertension is known to be intensive in the bovine species and sometimes leads to pathological cardiac repercussions. On the other hand, doubled-muscled cattle are predisposed ... [more ▼]

Hypoxic-induced pulmonary hypertension is known to be intensive in the bovine species and sometimes leads to pathological cardiac repercussions. On the other hand, doubled-muscled cattle are predisposed to develop hypoxaemia during exercise and with respiratory diseases. Therefore the purpose of this study was to investigate the cardiovascular response to acute hypoxia in double-muscled calves compared with calves of standard conformation. Pulmonary arterial pressure, electrocardiogram and blood temperature were simultaneously recorded, arterial blood was sampled for blood gas analysis and cardiac output was determined in six Friesian calves and six double-muscled calves of the Belgian White and Blue breed(BWB) when breathing air (fractional inspiratory oxygen concentration [FIO2]: 21 per cent) and when breathing a hypoxic gas mixture (FIO2: 10 per cent). All the absolute values of the measured parameters were significantly (P less than or equal to 0.001) different between the two breeds, except heart rate and arterial blood gas values. The pattern of hypoxic-induced decrease in arterial PO2 was similar in the two breeds of calves, suggesting that the pulmonary exchange capacities during hypoxia are no less efficient in double-muscled calves than in calves of standard conformation. Similarly, the percentage of variation of the mean pulmonary arterial pressure from its normoxic to its hypoxic value was the same in the two breeds of calves, suggesting that double-muscled calves are not predisposed to develop a more precocious or more intense pulmonary hypertension for a given level of hypoxaemia. The significantly smaller normoxic and hypoxic cardiac index and stroke index found in BWB compared with Friesian calves was interpreted as a less efficient cardiac function in double-muscled subjects. [less ▲]

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See detailCytogenetic Changes in Hepatocarcinomas from Rats Treated with Chronic Exposure to Diethylnitrosamine
Herens, Christian ULg; Alvarez Gonzalez, Maria-Luz ULg; Barbason, Hervé ULg

in Cancer Genetics & Cytogenetics (1992), 60(1), 45-52

Cytogenetic analysis of rat hepatocarcinomas obtained after diethylnitrosamine (DEN) exposure showed a wide variety of numerical and structural chromosomal changes: 53 of 86 hepatocellular carcinomas ... [more ▼]

Cytogenetic analysis of rat hepatocarcinomas obtained after diethylnitrosamine (DEN) exposure showed a wide variety of numerical and structural chromosomal changes: 53 of 86 hepatocellular carcinomas showed at least one recurrent chromosomal aberration. Some of these recurrent changes occurred in several tumors. Chromosomes 1, 3, 11, and 12 were abnormal in more than 30% of the carcinomas; chromosomes 2, 4, 5, and 10 were abnormal in 10%. Moreover, chromosomes 1 and 10 were generally lost or deleted and chromosome 3, 4, and 11 were very often gained. The most frequent anomaly was loss of chromosome 1 which was observed in 35% of the tetraploid cell populations. The occurrence in several tumors of recurrent chromosomal rearrangements as well as various repeated aneuploidies strongly suggests that these anomalies are implicated in the process of rat hepatocarcinogenesis induced by DEN treatment. [less ▲]

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See detailEffect of Repeated Intradermal Injections of Bovine Herpesvirus Type 1 Antigen on Seronegative Cattle
Thiry, Etienne ULg; Wellemans, G.; Limbourg, B. et al

in Veterinary Record : Journal of the British Veterinary Association (1992), 130(17), 372-5

Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity test ... [more ▼]

Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity test was assessed by the increase in skin thickness. The activity of the antigen was assessed in five animals which had a previous natural BHV-1 infection with clinical signs and seroconversion. Anti-BHV-1 antibodies were detected by seroneutralisation and an enzyme-linked immunoassay. Only one animal showed a significant but slight increase in skin thickness after the first test, but it was negative after a second test. The animals remained seronegative after the first test. Seroconversion was identified in 11 of the 43 animals (25 per cent) submitted to repeated delayed hypersensitivity tests. Five of 37 animals seroconverted after only two tests. The serological response was transient in seven of 11 seroconverted calves. Repeated hypersensitivity tests were therefore able to induce a serological response in seronegative calves but the response was weak and often transient. The test must therefore be applied cautiously to seronegative animals. [less ▲]

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See detailGROUND-BASED INFRARED MEASUREMENTS OF CARBONYL SULFIDE TOTAL COLUMN ABUNDANCES - LONG-TERM TRENDS AND VARIABILITY
RINSLAND, C. P.; Zander, Rodolphe ULg; Mahieu, Emmanuel ULg et al

in Journal of Geophysical Research (1992), 97(D5), 5995-6002

Total vertical column abundances of carbonyl sulfide (OCS) have been derived from time series of high-resolution infrared solar absorption spectra recorded at the National Solar Observatory McMath solar ... [more ▼]

Total vertical column abundances of carbonyl sulfide (OCS) have been derived from time series of high-resolution infrared solar absorption spectra recorded at the National Solar Observatory McMath solar telescope facility on Kitt Peak (altitude 2.09 km, latitude 31.9-degrees-N, longitude 111.6-degrees-W), southwest of Tucson, Arizona, and at the International Scientific Station of the Jungfraujoch (altitude 3.58 km, latitude 46.5-degrees-N, longitude 8.0-degrees-E), in the Swiss Alps. The analysis of both data sets is based on nonlinear least squares spectral fittings of narrow intervals centered on lines of the intense nu-3 band of OCS, the P(37) transition at 2045.5788 cm-1 and the P(15) transition at 2055.8609 cm-1, with a consistent set of spectroscopic line parameters. The Kitt Peak measurements, recorded on 30 different days between May 1977 and March 1991, show a 10% peak-to-peak seasonal cycle with a summer maximum and a winter minimum and a trend in the total column abundance equal to (0.1 +/- 0.2)% yr-1, 2-sigma. Jungfraujoch solar spectra recorded on 67 different days between October 1984 and April 1991 have been analyzed. The fitted trend in the Jungfraujoch total columns, (-0.1 +/- 0.5)% yr-1, 2-sigma, is consistent with the Kitt Peak trend results within the errors. The Jungfraujoch total columns show a more complex seasonal variation than noted in the Kitt Peak data. The mean of the daily averaged total columns, 8.44 x 10(15) molecules cm-2 above Kitt Peak and 6.41 x 10(15) molecules cm-2 above the Jungfraujoch station, correspond respectively to mean tropospheric mixing ratios of 0.54 +/- 0.04 and 0.52 +/- 0.04 parts per billion by volume; these values are consistent with previously reported remote and in situ measurements. Taken together, the results from the two sites indicate that there has been no significant change in the OCS total column abundance at northern mid-latitudes over the last decade. [less ▲]

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See detailA spectroscopic study of UM 673 A and B - On the size of Lyman-alpha clouds
Smette, A.; Surdej, Jean ULg; Shaver, P. A. et al

in Astrophysical Journal (1992), 389

The Ly-alpha forest spectra obtained for the A and B images of the gravitationally lensed high-redshift quasar UM 673 are investigated, and higher resolution data of the brightest (A) image are presented ... [more ▼]

The Ly-alpha forest spectra obtained for the A and B images of the gravitationally lensed high-redshift quasar UM 673 are investigated, and higher resolution data of the brightest (A) image are presented. In the 2-A resolution spectra, all the absorption lines detected at 5 sigma in the spectrum of the fainter B image are present in the A image, but two anticoincidences, i.e., two lines in A which do not have a counterpart in B at more than a 3-sigma confidence level, are detected. Given the fact that corresponding Ly-alpha lines in the spectra of A and B have their equivalent widths well correlated, this proves that both light beams actually cross the same clouds. Most of the velocity differences between corresponding lines are compatible with 0 km/s within the error bars, with a standard deviation of 17 km/s. For the two major heavy-element systems detected in the spectrum of UM 673, no significant difference between the corresponding lines in the two spectra are found. [less ▲]

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See detailPoint Mutations of Two Arginine Residues in the Streptomyces R61 Dd-Peptidase
Bourguignon-Bellefroid, Catherine; Joris, Bernard ULg; Van Beeumen, Jacques et al

in Biochemical Journal (1992), 283(1), 123-128

Incubation of the exocellular DD-carboxypeptidase/transpeptidase of Streptomyces R61 with phenylglyoxal resulted in a time-dependent decrease in the enzyme activity. This inactivation was demonstrated to ... [more ▼]

Incubation of the exocellular DD-carboxypeptidase/transpeptidase of Streptomyces R61 with phenylglyoxal resulted in a time-dependent decrease in the enzyme activity. This inactivation was demonstrated to be due to modification of the Arg-99 side chain. In consequence, the role of that residue was investigated by site-directed mutagenesis. Mutation of Arg-99 into leucine appeared to be highly detrimental to enzyme stability, reflecting a determining structural role for this residue. The conserved Arg-103 residue was also substituted by using site-directed mutagenesis. The modification to a serine residue yielded a stable enzyme, the catalytic properties of which were similar to those of the wild-type enzyme. Thus Arg-103, although strictly conserved or replaced by a lysine residue in most of the active-site penicillin-recognizing proteins, did not appear to fulfil any essential role in either the enzyme activity or structure. [less ▲]

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See detailPercutaneous Magnetic Stimulation of the Motor Cortex in Migraine
MAERTENS DE NOORDHOUT, Alain ULg; Pepin, Jean-Louis ULg; Schoenen, Jean ULg et al

in Electroencephalography and Clinical Neurophysiology (1992), 85(2), 110-5

We have used transcranial magnetic stimulation of the motor cortex interictally in 12 patients with unilateral classic migraine with sensorimotor auras and 10 patients with common migraine and unilateral ... [more ▼]

We have used transcranial magnetic stimulation of the motor cortex interictally in 12 patients with unilateral classic migraine with sensorimotor auras and 10 patients with common migraine and unilateral headache. In classic migraine, the threshold of activation of the FDI muscle by the cortical stimulus was significantly increased on the side of the auras, when compared to the unaffected side (P less than 0.01) and to normal subjects (P less than 0.01). The amplitude of EMG responses was also reduced in FDI on the affected side when compared to normals (P less than 0.02). Responses obtained in common migraine patients were normal on both sides. We suggest that some permanent subclinical dysfunction of the motor cortex might play a role in the pathogenesis of attacks of classic migraine with sensorimotor auras. [less ▲]

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See detailThe Stiffened Sheathings of Orthotropic Cylindrical Shells
Rigo, Philippe ULg

in Journal of Structural Engineering (1992)

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See detailPhysiopathologie du grêle court
Weber, Laurence ULg; Hamoir, Etienne ULg

in Revue Médicale de Liège (1992), 47(4), 202-9

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See detailMycotic aneurysm of the upper abdominal aorta ruptured into the stomach
Van Damme, Hendrik ULg; Belachew, M.; Damas, Pierre ULg et al

in Archives of Surgery (1992), 127(4), 478-482

We report a case of primary aortogastric fistula with erosion of a mycotic aneurysm of the upper abdominal aorta into the stomach. The patient was successfully operated on with an in situ aortoaortic tube ... [more ▼]

We report a case of primary aortogastric fistula with erosion of a mycotic aneurysm of the upper abdominal aorta into the stomach. The patient was successfully operated on with an in situ aortoaortic tube graft, incorporating the splanchnic vessels, and direct suture of the gastric erosion. [less ▲]

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See detailSmall bowel protection with "STEP". Silastic tissue expander prosthesis.
Coucke, Philippe ULg; Cuttat, J-F; Mirimanoff, R-O

in Strahlentherapie und Onkologie (1992), 168(4), 226229

Il s'agit d'un cas clinique d'un patient présentant un cancer du rectum et de la maladie de Crohn. Adjuvant treatment was considered to be mandatory on the basis of local extension. Traitement adjuvant a ... [more ▼]

Il s'agit d'un cas clinique d'un patient présentant un cancer du rectum et de la maladie de Crohn. Adjuvant treatment was considered to be mandatory on the basis of local extension. Traitement adjuvant a été considérée comme obligatoire sur la base de l'extension locale. Therefore a Silastic Tissue Expander Prosthesis (STEP), connected with a subcutaneously located self-sealing valve system, was introduced surgically to push small bowel up out of the treatment portals. Par conséquent, un Silastic Tissue Expander Prothèses (STEP), liée à un sous-cutanée située auto-obturant le système de valve, a été introduit chirurgicalement à pousser jusqu'à l'intestin grêle sur les portails de traitement. Patient received 55 Gy without any acute or late complication. Patient a reçu 55 Gy sans aucune complication aiguë ou tardive. This easy technique allows radiotherapy in conditions where complete elimination of small bowel outside the treatment volume is required. Cette technique simple permet de radiothérapie dans des conditions où l'élimination complète de l'intestin grêle en dehors du volume de traitement est nécessaire. [less ▲]

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See detailCytokine serum level during severe sepsis in human Il-6 as a marker of severity
Damas, Pierre ULg; Ledoux, Didier ULg; Nys, Monique ULg et al

in Annals of Surgery (1992), 215(4), 356-362

Forty critically ill surgical patients with documented infections were studied during their stay in an intensive care unit. Among these patients, 19 developed septic shock and 16 died, 9 of them from ... [more ▼]

Forty critically ill surgical patients with documented infections were studied during their stay in an intensive care unit. Among these patients, 19 developed septic shock and 16 died, 9 of them from septic shock. Interleukin 1 beta (IL-1 beta), tumor necrosis factor (TNF alpha), and interleukin 6 (IL-6) were measured each day and every 1 or 2 hours when septic shock occurred. Although IL-1 beta was never found, TNF alpha was most often observed in the serum at a level under 100 pg/mL except during septic shock. During these acute episodes TNF alpha level reached several hundred pg/mL, but only for a few hours. In contrast, IL-6 was always increased in the serum of acutely ill patients (peak to 500,000 pg/mL). There was a direct correlation between IL-6 peak serum level and TNF alpha peak serum level during septic shock and between IL-6 serum level and temperature or C-reactive protein serum level. Moreover, IL-6 correlated well with APACHE II score, and the mortality rate increased significantly in the group of patients who presented with IL-6 serum level above 1000 pg/mL. Thus, IL-6 appears to be a good marker of severity during bacterial infection. [less ▲]

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See detailEnseigner l'histoire dans l'enseignement technique et professionnel
Jadoulle, Jean-Louis ULg

in Bulletin de Clio (1992), n°92

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See detailTgf Beta 1 Expression Is Initiated in Adult Auditory Neurons by Sectioning of the Auditory Nerve
Lefebvre, P. P.; Martin, Didier ULg; Staecker, H. et al

in Neuroreport (1992), 3(4), 295-8

Neuronotrophic factors (e.g. basic fibroblast growth factor, bFGF and nerve growth factor, NGF) have been demonstrated to respectively promote survival and neuritogenesis in cultures of dissociated adult ... [more ▼]

Neuronotrophic factors (e.g. basic fibroblast growth factor, bFGF and nerve growth factor, NGF) have been demonstrated to respectively promote survival and neuritogenesis in cultures of dissociated adult rat spiral ganglia. Transforming growth factor beta (TGF beta 1) has been shown to modulate the response of cultured auditory neurons to bFGF through the induction of high affinity receptors for bFGF in the neurons. In this study, we show that TGF beta is expressed in situ by adult auditory neurons in response to traumatic injury (i.e. transection of the eighth cranial nerve). Based on these in vivo results and on the results from our previous in vitro studies, we propose that TFG beta 1 acts as an early autocrine signal involved in the response to injury by neurons of the peripheral auditor system. [less ▲]

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See detailHigh-rate of multiple genital HPV infections detected by DNA hybridization
Lauricella-Lefèbvre, M. A.; Piette, Jacques ULg; Lifrange, Eric ULg et al

in Journal of Medical Virology (1992), 36(4), 265-270

Cervical smears collected from 450 patients involved in a clinical follow-up of cervical human papillomaviruses (HPV) infections were screened for the presence of HPV 6b, 11, 16, and 18 DNA by both dot ... [more ▼]

Cervical smears collected from 450 patients involved in a clinical follow-up of cervical human papillomaviruses (HPV) infections were screened for the presence of HPV 6b, 11, 16, and 18 DNA by both dot blot and southern blot hybridization methods. Using very high stringency hybridization assays, the four HPV types could be easily distinguished by dot blotting. After a preliminary clinical sorting, 42.9% of the samples were found to be HPV-positive. Among the samples infected by a single HPV, type 16 was the most frequent (25.4% of the positive samples) followed by 6b (19.7%), 11 (8.3%), and 18 (7.2%). Double or even multiple infections by the different HPV types were detected at a very high rate (39.4% of the positive samples). [less ▲]

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See detailPredictors of Spontaneous Predischarge Ischemia Following Acute Myocardial Infarction
PIERARD, Luc ULg; Albert, Adelin ULg; Kulbertus, H. E.

in Clinical Cardiology : International Journal for Cardiovascular Diseases (1992), 15(4), 260-4

To determine if the occurrence and the consequences of spontaneous predischarge postinfarction ischemia could be predicted early after hospital admission, a consecutive series of patients with acute ... [more ▼]

To determine if the occurrence and the consequences of spontaneous predischarge postinfarction ischemia could be predicted early after hospital admission, a consecutive series of patients with acute myocardial infarction was studied and followed for 3 years. No patient was treated by thrombolysis. Spontaneous predischarge ischemia was defined as angina that occurred at rest before hospital discharge, at least 3 days after the acute event, and that was accompanied by electrocardiographic changes, but not by an increase in cardiac enzymes. Patients who died within the first 3 days were excluded from analysis. Among the 943 patients who survived at least 3 days, 165 (17.5%) had spontaneous ischemia before discharge. They had a higher 1-year post-hospital mortality (16 vs. 10%), but did not have significantly higher total 3-year mortality rates. Four independent, early available variables predictive of the occurrence of spontaneous ischemia were selected from a stepwise logistic discriminant analysis: history of angina before infarction, non-Q-wave infarct, absence of smoking, and higher age. Among the 165 patients with spontaneous ischemia, 3 independent variables predictive of 3-year mortality were selected stepwise: left ventricular function score, history of previous infarction, and absence of smoking. [less ▲]

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See detailImmunocytochemical Localization of Androgen Receptors in the Male Songbird and Quail Brain
Balthazart, Jacques ULg; Foidart, Agnès ULg; Wilson, E. M. et al

in Journal of Comparative Neurology (The) (1992), 317(4), 407-20

The distribution of androgen receptors was studied in the brain of the Japanese quail (Coturnix japonica), the zebra finch (Taeniopygia guttata), and the canary (Serinus canaria) by immunocytochemistry ... [more ▼]

The distribution of androgen receptors was studied in the brain of the Japanese quail (Coturnix japonica), the zebra finch (Taeniopygia guttata), and the canary (Serinus canaria) by immunocytochemistry with a polyclonal antibody (AR32) raised in rabbit against a synthetic peptide corresponding to a sequence located at the N-terminus of the androgen receptor molecule. In quail, androgen receptor-immunoreactive cells were observed in the nucleus intercollicularis and in various nuclei of the preoptic-hypothalamic complex, namely, the nucleus preopticus medialis, the ventral part of the nucleus anterior medialis hypothalami, the nucleus paraventricularis magnocellularis, the nucleus ventromedialis hypothalami, and the tuberal hypothalamus. In the two songbird species, labeled cells were also observed in various nuclei in the preoptic-hypothalamic region, in the nucleus taeniae, and in the nucleus intercollicularis. Additional androgen receptor-immunoreactive cells were present in the androgen-sensitive telencephalic nuclei that are part of the song control system. These immunoreactive cells filled and outlined the boundaries of the hyperstriatum ventrale, pars caudalis, nucleus magnocellularis neostriatalis anterioris (both in the lateral and medial subdivisions), and nucleus robustus archistriatalis. The immunoreactive material was primarily present in cell nuclei but a low level of immunoreactivity was also clearly detected in cytoplasm in some brain areas. These studies demonstrate, for the first time, that androgen receptors can be detected by immunocytochemistry in the avian brain and the results are in general agreement with the binding data obtained by autoradiography with tritiated dihydrotestosterone. Immunocytochemical methods offer several advantages over autoradiography and their use for the study of the androgen receptor will greatly facilitate the analysis of steroid-sensitive systems in the avian brain. [less ▲]

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See detailBasement Membrane Components (Matrigel) Promote the Tumorigenicity of Human Breast Adenocarcinoma Mcf7 Cells and Provide an in Vivo Model to Assess the Responsiveness of Cells to Estrogen
Noël, Agnès ULg; Simon, N.; Raus, J. et al

in Biochemical Pharmacology (1992), 43(6), 1263-7

The ability to transplant human tumors into athymic nude mice allows studies of tumor cells in vivo. However, after s.c. injection the incidence of tumor and metastases in nude mice is frequently low. We ... [more ▼]

The ability to transplant human tumors into athymic nude mice allows studies of tumor cells in vivo. However, after s.c. injection the incidence of tumor and metastases in nude mice is frequently low. We have studied the tumorigenicity in nude mice of estradiol (E2)-sensitive breast adenocarcinoma MCF7 cells. Matrigel, an extract of basement membrane proteins, induces rapid tumor development after s.c. injection of MCF7 cells. In the absence of this matrice, MCF7 cells failed to induce tumor growth. In this in vivo model, MCF7 cells were analysed for their E2 sensitivity. Two weeks after inoculation in the presence of matrigel, cells formed growing tumors in intact mice supplemented with E2. In ovariectomized or untreated mice, tumor appearance was delayed and the growth level was very low. Thus, MCF7 cells formed tumors in the absence of E2 but retained in vivo their responsiveness to estrogen. Growing human tumors in nude mice provides a rapid and useful model for testing the sensitivity of cells to hormone. [less ▲]

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See detailPrimary and Predicted Secondary Structures of the Actinomadura R39 Extracellular DD-Peptidase, a Penicillin-Binding Protein (PBP) Related to the Escherichia coli PBP4
Granier, Benoît; Duez, Colette ULg; Englebert, Serge et al

in Biochemical Journal (1992), 282(Pt 3), 781-788

As derived from gene cloning and sequencing, the 489-amino-acid DD-peptidase/penicillin-binding protein (PBP) produced by Actinomadura R39 has a primary structure very similar to that of the Escherichia ... [more ▼]

As derived from gene cloning and sequencing, the 489-amino-acid DD-peptidase/penicillin-binding protein (PBP) produced by Actinomadura R39 has a primary structure very similar to that of the Escherichia coli PBP4 [Mottl, Terpstra & Keck (1991) FEMS Microbiol. Lett. 78, 213-220]. Hydrophobic-cluster analysis of the two proteins shows that, providing that a large 174-amino-acid stretch is excluded from the analysis, the bulk of the two polypeptide chains possesses homologues of the active-site motifs and secondary structures found in the class A β-lactamase of Streptomyces albus G of known three-dimensional structure. The 74-amino-acid insert occurs at equivalent places in the two PBPs, between helices α2 and α3, away from the active site. Such an insert is unique among the penicilloyl serine transferases. It is proposed that the Actinomadura R39 PBP and E. coli PBP4 form a special class, class C, of low-Mr PBPs/DD-peptidases. A vector has been constructed and introduced by electrotransformation in the original Actinomadura R39 strain, allowing high-level expression and secretion of the DD-peptidase/PBP (250 mg . 1-1). The gene encoding the desired protein is processed differently in Actinomadura R39 and Streptomyces lividans. Incorrect processing in Streptomyces lividans leads to a secreted protein which is inert in terms of DD-peptidase activity and penicillin-binding capacity. [less ▲]

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See detailPurification, Characterization, and Nucleotide Sequence of the Thermolabile Alpha-Amylase from the Antarctic Psychrotroph Alteromonas Haloplanctis A23
Feller, Georges ULg; Lonhienne, T.; Deroanne, Christophe ULg et al

in Journal of Biological Chemistry (1992), 267(8), 5217-21

The alpha-amylase excreted by the antarctic bacterium Alteromonas haloplanctis was purified and the corresponding amy gene was cloned and sequenced. N- and C-terminal amino acid sequencing were used to ... [more ▼]

The alpha-amylase excreted by the antarctic bacterium Alteromonas haloplanctis was purified and the corresponding amy gene was cloned and sequenced. N- and C-terminal amino acid sequencing were used to establish the primary structure of the mature A. haloplanctis alpha-amylase which is composed of 453 amino acids with a predicted Mr of 49,340 and a pI of 5.5. Three Ca2+ ions are bound per molecule and its activity is modulated by chloride ions. Within the four consensus sequences, Asp-174, Glu-200, and Asp-264 are the proposed catalytic residues. The psychrotrophic A. haloplanctis alpha-amylase is characterized by a high amylolytic activity at low temperatures, a reduced apparent optimal temperature, and typical thermodynamic activation parameters A. haloplanctis alpha-amylase has also a low thermal stability as demonstrated by the temperature effect on both activity and secondary structure. It is suggested that structure flexibility and lower sensitivity of secondary structure to temperature variations in the low temperature range are the main structural adaptations of the psychrotrophic enzyme. The unusual stacking of small amino acids around the catalytic residues is proposed as a factor inducing active site flexibility and concomitant high activity of the enzyme at low temperatures. [less ▲]

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See detailModular Design of the Enterococcus Hirae Muramidase-2 and Streptococcus Faecalis Autolysin
Joris, Bernard ULg; Englebert, Serge; Chu, Chien-Peng et al

in FEMS Microbiology Letters (1992), 91(3), 257-264

The mature forms of the extracellular muramidase-2 of Enterococcus hirae and Streptococcus faecalis autolysin have very similar primary structures. Each consists of an active-site-containing N-terminal ... [more ▼]

The mature forms of the extracellular muramidase-2 of Enterococcus hirae and Streptococcus faecalis autolysin have very similar primary structures. Each consists of an active-site-containing N-terminal domain fused to a multiple-repeat C-terminal domain. Polypeptide segments occurring at equivalent places in these two bacterial wall lytic enzymes have homologues in two phage lysozymes and in three functionally unrelated proteins, illustrating the principle that protein molecules frequently are constructed from modules that are linked in a single polypeptide chain. [less ▲]

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See detailLe vieillissement de l'épiderme
Pierard, Gérald ULg; Nikkels, Arjen ULg; Fazaa, B. et al

in Médecine et Hygiène (1992), 50(1924), 664-666

Skin aging is defined by the progressive loss in homeostatic capacity of the epidermis and dermis to respond to various stimuli. Photoaging is a peculiar type of aging which occurs on sun exposed areas ... [more ▼]

Skin aging is defined by the progressive loss in homeostatic capacity of the epidermis and dermis to respond to various stimuli. Photoaging is a peculiar type of aging which occurs on sun exposed areas. We review the structural and functional alterations of the epidermis in relation to the origin of aging. [less ▲]

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See detailNeuroanatomical Specificity in the Autoregulation of Aromatase-Immunoreactive Neurons by Androgens and Estrogens: An Immunocytochemical Study
Balthazart, Jacques ULg; Foidart, Agnès ULg; Surlemont, C. et al

in Brain Research (1992), 574(1-2), 280-90

Testosterone (T) increases brain aromatase activity (AA) in quail and other avian and mammalian species. It was shown both in quail and in rat that this enzymatic induction results from a synergistic ... [more ▼]

Testosterone (T) increases brain aromatase activity (AA) in quail and other avian and mammalian species. It was shown both in quail and in rat that this enzymatic induction results from a synergistic action of androgens and estrogens. These studies provide little information on possible anatomical or cellular specificity of the effect. Using a polyclonal antiserum against human placental aromatase, we have previously identified aromatase-immunoreactive (ARO-ir) neurons in the quail brain and demonstrated that T increases the number of ARO-ir cells in the quail preoptic area (POA) supporting previous evidence that T increases AA in the brain. However, which T metabolites are involved, the actual mechanism of regulation and the possibility of anatomical specificity for these effects are not yet clear. In the present study, we disassociated the effects of androgens and estrogens in aromatase induction by comparing ARO-ir neurons of quail treated with T alone or T in the presence of a potent aromatase inhibitor (R76713), which has been shown to depress AA levels and to suppress T-activated copulatory behavior. T increased the number of ARO-ir cells in POA, bed nucleus striae terminalis (BNST) and tuberal hypothalamus (Tu). The T effect was inhibited by concurrent treatment with aromatase inhibitor in Tu, but not in POA and BNST. This differential effect of the aromatase inhibitor fits in very well with our previous studies of the co-localization of aromatase and estrogen receptors. The T effect was blocked by R76713 in areas where ARO-ir and estrogen receptor-ir are generally co-localized (Tu) and was not affected in areas with mainly ARO-ir positive, estrogen receptor-ir negative cells (POA, BNST). This suggests anatomical differences in the expression or clearance of aromatase which may be differentially sensitive to androgens and estrogens and dependent upon the presence of sex steroid receptors. [less ▲]

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See detailImportance of the His-298 Residue in the Catalytic Mechanism of the Streptomyces R61 Extracellular Dd-Peptidase
Hadonou, Ayaovi M.; Jamin, Marc; Adam, Maggy et al

in Biochemical Journal (1992), 282(Pt 2), 495-500

Among the active-site-serine penicillin-recognizing proteins, the Streptomyces R61 extracellular DD-peptidase is the only one to have a His-Thr-Gly sequence [instead of Lys-Thr(Ser)-Gly] in 'box' VII. The ... [more ▼]

Among the active-site-serine penicillin-recognizing proteins, the Streptomyces R61 extracellular DD-peptidase is the only one to have a His-Thr-Gly sequence [instead of Lys-Thr(Ser)-Gly] in 'box' VII. The His residue was replaced by Gln or Lys. Both mutations induced a marked decrease in the rates of both tripeptide substrate hydrolysis and acylation by benzylpenicillin and cephalosporin C. The rate of hydrolysis of the thioester hippuryl thioglycollate was less affected. The most striking result was the disproportionate loss of transpeptidation properties by both mutants, indicating an important role of His-298 in this reaction. We believe that this result represents the first modification of a DD-peptidase leading to a specific decrease of the transpeptidation-to-hydrolysis ratio. [less ▲]

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See detailWR 134 line-profile variability revisited
Vreux, Jean-Marie ULg; Gosset, Eric ULg; Bohannan, B. et al

in Astronomy and Astrophysics (1992), 256

Line profile variations of WR 134 are analyzed on more than 200 spectra collected at Haute Provence Observatory and at Kitt Peak National Observatory. No conclusive evidence is found for a periodic ... [more ▼]

Line profile variations of WR 134 are analyzed on more than 200 spectra collected at Haute Provence Observatory and at Kitt Peak National Observatory. No conclusive evidence is found for a periodic behavior. However, for some frequencies, for example, the ones close to nu = (n x 0.5)/d, the data are ill-distributed, and the existence of a related periodic phenomenon cannot be completely ruled out. This does not mean that the variability presents a purely random behavior. Several new characteristics of the observed deformations are extracted from the analysis of the spectra and it is tentatively suggested that these could be the signature of a bipolar flow. [less ▲]

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See detailImportance of the Two Tryptophan Residues in the Streptomyces R61 Exocellular Dd-Peptidase
Bourguignon-Bellefroid, Catherine; Wilkin, Jean-Marc; Joris, Bernard ULg et al

in Biochemical Journal (1992), 282(Pt 2), 361-367

Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzyme's two tryptophan residues was investigated by ... [more ▼]

Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzyme's two tryptophan residues was investigated by site-directed mutagenesis. Trp271 was replaced by Leu. The modification yielded a stable enzyme whose structural and catalytic properties were similar to those of the wild-type protein. Thus the Trp271 residue, though almost invariant among the beta-lactamases of classes A and C and the low-Mr penicillin-binding proteins, did not appear to be essential for enzyme activity. Mutations of the Trp233 into Leu and Ser strongly decreased the enzymic activity, the affinity for beta-lactams and the protein stability. Surprisingly, the benzylpenicilloyl-(W233L)enzyme deacylated at least 300-fold more quickly than the corresponding acyl-enzyme formed with the wild-type protein and gave rise to benzylpenicilloate instead of phenylacetylglycine. This mutant DD-peptidase thus behaved as a weak beta-lactamase. [less ▲]

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See detailLight curve of the Ap star TW Columbae
Renson, Pierre ULg; Manfroid, Jean ULg

in Astronomy and Astrophysics (1992), 256

The uvby light curves of the Ap star TW Col are analyzed. The presence of a small-scale dip in the curves appears to be in conflict with a simple spotted-rotator model. TW Col is the second star, after 46 ... [more ▼]

The uvby light curves of the Ap star TW Col are analyzed. The presence of a small-scale dip in the curves appears to be in conflict with a simple spotted-rotator model. TW Col is the second star, after 46 Eri, for which such a phenomenon seems to be confirmed. [less ▲]

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See detailCloning and Nucleotide Sequence of the Gene Encoding the Gamma-D-Glutamyl-L-Diamino Acid Endopeptidase Ii of Bacillus Sphaericus
Hourdou, Marie-Laure; Duez, Colette ULg; Joris, Bernard ULg et al

in FEMS Microbiology Letters (1992), 91(2), 165-170

The gene encoding the Bacillus sphaericus gamma-D-glutamyl-L-diamino acid endopeptidase II, a cytoplasmic enzyme involved in cell sporulation [1], contains the information for a 271-amino acid protein ... [more ▼]

The gene encoding the Bacillus sphaericus gamma-D-glutamyl-L-diamino acid endopeptidase II, a cytoplasmic enzyme involved in cell sporulation [1], contains the information for a 271-amino acid protein devoid of a signal peptide. The endopeptidase lacks sequence relatedness with other proteins of known primary structure except that its C-terminal region has significant similarity with the C-terminal region of the 54-kDa P54 protein of Enterococcus faecium, of unknown function [2]. [less ▲]

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See detailSignalement archéologique
Longrée, Dominique ULg

in Vie Archéologique - Info (1992)

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See detailOptimalisation d'un séjour linguistique par la pédagogie par projet. Description et commentaire d'une expérience réalisée en Promotion sociale.
Simons, Germain ULg

in Education-Formation (1992), 226

Cet article décrit et commente une expérience réalisée dans le cadre d'un cours d'anglais (3e CTSS) en Promotion sociale à l'IPSCF de Waremme. Il s'agit d'INTEGRER un séjour linguistique (York) au cours d ... [more ▼]

Cet article décrit et commente une expérience réalisée dans le cadre d'un cours d'anglais (3e CTSS) en Promotion sociale à l'IPSCF de Waremme. Il s'agit d'INTEGRER un séjour linguistique (York) au cours d'anglais/langue étrangère depuis le choix du projet jusqu'à son exploitation et évaluation en passant par la planification et la réalisation du séjour. Ce type d'approche pédagogique, qui met l'apprenant en contact direct avec la langue-cible et sa culture, présente un intérêt évident pour un apprentissage communicatif de la langue étrangère. [less ▲]

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See detailEffect of intravenous and aerosol administration of 5-hydroxytryptamine on pulmonary function values in healthy calves
Desmecht, Daniel ULg; Linden, Annick ULg; Rollin, Frédéric ULg et al

in American Journal of Veterinary Research (1992), 53(3), 315-320

Effects of IV and aerosol administration of 5-hydroxytryptamine (5-HT) on ventilation, pulmonary mechanics values, pulmonary arterial pressure, and heart rate were investigated in healthy unsedated ... [more ▼]

Effects of IV and aerosol administration of 5-hydroxytryptamine (5-HT) on ventilation, pulmonary mechanics values, pulmonary arterial pressure, and heart rate were investigated in healthy unsedated Friesian calves. Minute volume increased significantly, mainly because of an increase in respiratory rate. Except for total pulmonary resistance after bolus injection, continuous administration of 5-HT given by either route caused significant alterations of lung dynamic compliance and total pulmonary resistance, the former decreasing to one-fifth of its baseline value and the latter increasing twofold. Pulmonary arterial pressure increased significantly, whatever the speed or route of administration. Administration of a bolus did not affect heart rate, whereas continuous IV administration of 5-HT as well by perfusion or by aerosol resulted in sustained tachycardia. It was concluded that 5-HT induces reversible bronchoconstriction and pulmonary vasoconstriction in healthy unsedated calves, 5-HT-induced functional alterations depend on the speed of administration, and excess of 5-HT production or depression in uptake by the lungs during bovine respiratory tract diseases could contribute to pulmonary dysfunction. [less ▲]

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See detailInfluence of Estrogen Replacement Therapy on Endogenous Calcitonin Production Rates
Reginster, Jean-Yves ULg; Deroisy, Rita ULg; Fontaine, M. A. et al

in Gynecological Endocrinology : The Official Journal of the International Society of Gynecological Endocrinology (1992), 6(1), 65-71

Calcitonin is now a well-accepted therapy for inhibition of bone loss, both in the first years of menopause and in established osteoporosis. However, its exact role in the pathogenesis of that disease as ... [more ▼]

Calcitonin is now a well-accepted therapy for inhibition of bone loss, both in the first years of menopause and in established osteoporosis. However, its exact role in the pathogenesis of that disease as well as the interactions between calcitonin production and estrogen metabolism remain unsolved. In order to clarify the influence of estrogen replacement therapy (ERT) on calcitonin secretory capacity, we measured whole plasma immunoreactive calcitonin basal levels, metabolic clearance rates and production rates in a group of postmenopausal women, before and after a daily intake for 28 days of 0.625 mg/day of conjugated equine estrogens, and again 4 weeks after the withdrawal of that estrogen replacement therapy. No significant changes appeared in immunoreactive calcitonin or immunoreactive calcitonin metabolic clearance rate but the production rate significantly increased over the 28 days (mean +/- SEM, from 21.3 +/- 5.1 pg/ml to 25.2 +/- 5.9 pg/ml, p less than 0.05), and then decreased 4 weeks after therapy was withdrawn to the initial level (17.9 +/- 3.6 pg/ml). We concluded that estrogen replacement therapy significantly increases calcitonin secretory capacity. This confirms the interactions between calcitonin production and estrogen metabolism, and may provide an explanation concerning the mode of action of estrogen replacement therapy in prevention of postmenopausal bone loss. [less ▲]

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See detailThe new gravitational lens candidate Q 1208+1011 and the importance of high quality data.
Magain, Pierre ULg; Surdej, Jean ULg; Vanderriest, C. et al

in The Messenger (1992), 67

Not Available

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See detailLe langage universel de la communication cellulaire et sa pathologie. Les chemins communs du langage intercellulaire
Geenen, Vincent ULg; Robert, F.; Legros, Jean-Jacques ULg et al

in Revue Médicale de Liège (1992), 47(3), 109-15

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See detailL'implantation placentaire humaine
Foidart, Jean-Michel ULg

in Bulletin de l'Académie Nationale de Médecine (1992), 176(3), 303-7308-9

Like malignant tumor cells, cells of the trophoblast, of the blastocyst and of the hemochorial placenta are highly invasive. This invasiveness is essential for implantation and penetration of the ... [more ▼]

Like malignant tumor cells, cells of the trophoblast, of the blastocyst and of the hemochorial placenta are highly invasive. This invasiveness is essential for implantation and penetration of the epithelial and connective tissues of the endometrium. According to Hertig's data and the observations (henceforth classical) of the Carnegie Foundation, it is commonly admitted that trophoblastic cells penetrate rapidly the blood vessels of the maternal endometrium, open them and allow the establishment of a communication between maternal circulation and fetal tissues from the 20th day after fertilization. As suggested in the classical textbooks of Embryology, the hemochorial placentation would be established very early in human pregnancy. These observations (essentially histological) are actually contradicted by clinical observations performed by Doppler contact echography and by dynamic observations with a microvideo camera implanted in the intervillous placental chamber. Echographic observations coupled with the study of Doppler signals suggest the absence of placental maternal vascularization during the first trimester of pregnancy. It is possible to mount an optical system between the membranes and the uterine wall up to the placental tissue to observe the placental circulation. This direct observation concludes to the absence of maternal vascularization in the intervillous chamber during the first trimester of pregnancy. At the beginning of the 13th week of gestation, uterine arteries open directly into the intervillous chamber ensuring the direct perfusion of placental villi. These informations allow to suggest that the placenta becomes hemochorial at the beginning of the second trimester of pregnancy. [less ▲]

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See detailLa cultura en México (1950-1972)
Vanden Berghe, Kristine ULg

in L'ordinaire. Mexique Amérique Centrale (1992), 138

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See detailEarly neurogenesis and teratogenesis in whole mouse embryo cultures. Histochemical, immunocytological and ultrastructural study of the premigratory neuronal-glial units in normal mouse embryo and in mouse embryos influenced by cocaine and retinoic acid
Gressens, Pierre; Gofflot, Françoise; Van Maele-Fabry, Genevieve et al

in Journal of Neuropathology and Experimental Neurology (1992), 51(2), 206-219

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See detailThe association of astrocytoma and pituitary adenoma in a patient with alcaptonuria.
Abs, R.; Van Vyve, M.; Willems, P. J. et al

in Journal of the Neurological Sciences (1992), 108(1), 32-34

A female patient with a juvenile pilocytic astrocytoma and a pituitary adenoma is described. The patient also has alcaptonuria, a rare inborn error of metabolism with autosomal recessive mode of ... [more ▼]

A female patient with a juvenile pilocytic astrocytoma and a pituitary adenoma is described. The patient also has alcaptonuria, a rare inborn error of metabolism with autosomal recessive mode of inheritance. The association of these three disorders has never been reported previously. The possible existence of a common genetic factor in the development of both tumors and alcaptonuria is discussed. [less ▲]

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See detailMécanismes de communication cellulaire dans le système nerveux périphérique en régénération
Leprince, Pierre ULg; Delree, P.; Rogister, Bernard ULg et al

in Revue Médicale de Liège (1992), 47(3), 115-8

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See detailStreptomyces Albus G Serine Beta-Lactamase. Probing of the Catalytic Mechanism Via Molecular Modelling of Mutant Enzymes
Lamotte-Brasseur, Josette; Jacob-Dubuisson, Françoise; Dive, Georges ULg et al

in Biochemical Journal (1992), 282(Pt 1), 189-195

In previous studies, several amino acids of the active site of class A , β-lactamases have been modified by site-directed mutagenesis. On the basis of the catalytic mechanism proposed for the Streptomyces ... [more ▼]

In previous studies, several amino acids of the active site of class A , β-lactamases have been modified by site-directed mutagenesis. On the basis of the catalytic mechanism proposed for the Streptomyces albus G , β-lactamase [Lamotte- Brasseur, Dive, Dideberg, Charlier, Frere & Ghuysen (1991) Biochem. J. 279, 213-221], the influence that these mutations exert on the hydrogen-bonding network of the active site has been analysed by molecular mechanics. The results satisfactorily explain the effects of the mutations on the kinetic parameters of the enzyme's activity towards a set of substrates. The present study also shows that, upon binding a properly structured ,β-lactam compound, the impaired cavity of a mutant enzyme can readopt a functional hydrogen-bonding-network configuration. [less ▲]

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See detailThermospheric odd nitrogen
Gérard, Jean-Claude ULg

in Planetary and Space Science (1992), 40

The photochemistry of NO, N(2D) and N(4S) and results of recent space measurements of their density distribution are discussed. In particular, the role of the reaction between metastable N(2D) atoms and ... [more ▼]

The photochemistry of NO, N(2D) and N(4S) and results of recent space measurements of their density distribution are discussed. In particular, the role of the reaction between metastable N(2D) atoms and O2 as a source of O(1D) is discussed in the light of laboratory and aeronomical observations. Global satellite measurements are compared with results of 2D and 3D models including transport. The possibility of explaining the odd nitrogen observations gathered in the Venusian and Martian thermospheres with the current understanding of the terrestrial models adapted to CO2-rich atmospheres is examined. It is concluded that understanding of the processes governing the distribution of odd nitrogen in terrestrial planets is generally satisfactory, although several aspects require further quantitative investigation. [less ▲]

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See detailEffects of Alpha-Methyl-Para-Tyrosine on Monoamine Levels in the Japanese Quail: Sex Differences and Testosterone Effects
Balthazart, Jacques ULg; Foidart, Agnès ULg; Sante, P. et al

in Brain Research Bulletin (1992), 28(2), 275-88

Experiments were performed to obtain more information on the regulation by steroids of catecholaminergic systems in the brain of Japanese quail. Dose-response and time-response experiments were first ... [more ▼]

Experiments were performed to obtain more information on the regulation by steroids of catecholaminergic systems in the brain of Japanese quail. Dose-response and time-response experiments were first performed to determine optimal conditions for measuring turnover in the quail brain. The norepinephrine and dopamine turnover were then estimated in microdissected brain nuclei of birds that were either sexually mature or gonadectomized or gonadectomized and treated with testosterone. Two major facts that bear direct relationship with the control of masculine reproductive behavior were demonstrated. On one hand, the dopamine turnover in the medial preoptic nucleus, a sexually dimorphic brain structure which is critically implicated in the control of copulatory behavior was much higher in male than in female quail irrespective of the hormonal condition of the birds. On the other hand, norepinephrine concentrations appeared to be higher in several nuclei of the female brain by comparison with males. These sex differences might represent part of the causal factors that underlie the sex dimorphism in reproductive behavior in quail. [less ▲]

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