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See detailAnomalous behavior of the irreversible magnetization and time relaxation in YBa(2)Cu(3)O(7) single crystals with splayed tracks
Silhanek, Alejandro ULg; Niebieskikwiat, D.; Civale, L. et al

in Physical Review. B : Condensed Matter (1999), 60(18), 13189-13195

We have studied the angular dependence of the irreversible magnetization and its time relaxation in YBa(2)Cu(3)O(7) Single crystals with one or two families of columnar defects inclined with respect to ... [more ▼]

We have studied the angular dependence of the irreversible magnetization and its time relaxation in YBa(2)Cu(3)O(7) Single crystals with one or two families of columnar defects inclined with respect to the c axis. At high magnetic fields, the magnetization shows the usual maximum centered at the mean tracks' orientation and an associated minimum in the normalized relaxation rate. In contrast, at low fields we observe an anomalous local minimum in the magnetization and a maximum in the relaxation rate. We present a model to explain this anomaly based on the slowing down of the creep processes arising from the increase of the vortex-vortex interactions as the applied field is tilted away from the mean tracks' direction. [S0163-1829(99)11841-1]. [less ▲]

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See detailUsing Confocal Microscopy to Study Virus Binding and Entry into Cells
Vanderplasschen, Alain ULg; Smith, G. L.

in Methods in Enzymology (1999), 307

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See detailDe nouveaux acteurs dans la physiopathologie du metabolisme de l'eau: les aquaporines
Pequeux, Christel ULg; Brilot, Fabienne; Martens, Henri ULg et al

in Revue Médicale de Liège (1999), 54(11), 867-74

Aquaporins are transmembrane proteins mediating water transport across plasma membrane of animal, vegetal or bacterial cells. Among the ten aquaporins known in mammals, six are located in kidney and take ... [more ▼]

Aquaporins are transmembrane proteins mediating water transport across plasma membrane of animal, vegetal or bacterial cells. Among the ten aquaporins known in mammals, six are located in kidney and take part in urine concentration. AQP2 is vasopressin regulated, it is the only family member to be implicated in human pathology, such as nephrogenic diabetes insipidus, congestive heart failure, hepatic cirrhosis, nephrotic syndrome or SIADH. Aquaporins are expressed in a wide variety of tissues, such as brain or gastrointestinal tractus, and suggest a role in water tissue exchange, but their real function is still not define. To know the physiological impact of aquaporins, AQP1, AQP3, AQP4 and AQP5 knockout mice have been created and their phenotype analysed. [less ▲]

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See detailMixed monolayers of gluten-emulsifiers at the air-water interface
Balla, A.; Blecker, Christophe ULg; Razafindralambo, Hary ULg et al

in Biopolymer Science: Food And Non Food Applications (1999), (91), 213-216

The interactions and miscibility analysis of gluten-emulsifiers mixed film show that those films are not completely miscible as demonstrated by the presence of both two transition zones and also by the ... [more ▼]

The interactions and miscibility analysis of gluten-emulsifiers mixed film show that those films are not completely miscible as demonstrated by the presence of both two transition zones and also by the deviation of the mean molecular areas of mixed films from the values calculated according to the rule of additivity (GAINES, 1966). Furthermore, the free energy excess of mixing is positive in presence of emulsifiers, confirming the partial miscibility of gluten emulsifier monolayers. [less ▲]

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See detailComparison of necrotoxigenic Escherichia coli isolates from farm animals and humans
Mainil, Jacques ULg; Jacquemin, E.; Pohl, P. et al

in Veterinary Microbiology (1999), 70

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See detailThe use of different dual X-ray absorptiometry brands in a multicenter clinical trial
Slosman, DO; Provedini, DM; Meunier, PJ et al

in Journal of Clinical Densitometry : The Official Journal of the International Society for Clinical Densitometry (1999), 2

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See detailPrise en charge médico-chirurgicale des enfants cardiopathes: résultats de l'expérience liégeoise
Massin, M.M.; RADERMECKER, Marc ULg; Jalali, H. et al

in Revue Médicale de Liège (1999), 54(2), 95-9

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and ... [more ▼]

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and interventional cardiac catheterization, and of the cardiac surgery in the 123 cardiac children who were referred to us during the three first years of operation of our medico-surgical team. RESULTS: 89 children underwent a catheterization, including 12 therapeutic interventions, whereas 68 were operated. In the two domains, the results are completely comparable with those of the established centers. CONCLUSIONS: The widening of the activity of paediatric cardiology and the creation of an activity of pediatric cardiac surgery in Liege appeared important to us for the quality of management of the cardiac children. This goal could be reached only if our results were comparable with those of the literature. The bet seems to be held. The ultimate objective is to contribute by an adequate policy of management to the quality of life and the health of the cardiac children. [less ▲]

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See detailSimultaneous Determination of Deoxyribonucleoside in the Presence of Ribonucleoside Triphosphates in Human Carcinoma Cells by High-Performance Liquid Chromatography
Decoster, L-A; Chen, X; Lejeune, F et al

in Analytical Biochemistry (1999), 270

Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in cells by HPLC is an analytical challenge since the concentration of dNTP present in mammalian cells is several orders ... [more ▼]

Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in cells by HPLC is an analytical challenge since the concentration of dNTP present in mammalian cells is several orders of magnitude lower than the corresponding NTP. Hence, the quantitation of dNTP in cells is generally performed after selective oxidation or removal of the major NTP. The procedures reported so far are lengthy and cumbersome and do not enable the simultaneous determination of NTP. We report the development of a simple, direct HPLC method for the simultaneous determination of dNTP and NTP in colon carcinoma WiDr cell extracts using a stepwise gradient elution ion-pairingHPLCwith uv detection at 260 nm and with a minimal chemical manipulation of cells. Exponentially growing WiDr cells were harvested by centrifugation, rinsed with phosphate- buffered saline, and carefully counted. The pellets were suspended in a known volume of ice-cold water and deproteinized with an equal volume of 6% trichloroacetic acid. The acid cell extracts (corresponding to 2.53 106 cells/100 ml) were centrifuged at 13,000g for 10 min at 4°C. The resulting supernatants were stored at 280°C prior to analysis. Aliquots (100 ml) were neutralized with 4.3 ml saturated Na2CO3 solution prior the injection of 40 ml onto the HPLC column (injection speed 250 ml/min). Chromatographic separations were performed using two Symmetry C18 3.5-mm (2 3 3.9 3 150 mm) columns (Waters), connected in series equipped with a Sentry guard column (3.9 3 20 mm i.d.) filled with the same packing material. The HPLC columns were kept at 30°C. The mobile phase was delivered at a flow rate of 0.5 ml/min, with the following stepwise gradient elution program: % solvent A/solvent B, 100/0 at 0 min 3 100/0 at 1 min 3 36/64 at 5 min 3 31/69 at 90 min 3 31/69 at 105 min 3 0/100 at 106 min 3 0/100 at 120 min; 50/50 MeOH/solvent B from 121 to 130 min; 100% solvent A from 131 to 160 min. Solvent A contained 0.01 M KH2PO4, 0.01 M tetrabutylammonium chloride, and 0.25% MeOH and was adjusted to pH 7.0 (550 ml 10 N NaOH for 1 liter solvent A). Solvent B consisted of 0.1 MKH2PO4, 0.028Mtetrabutylammonium chloride, and 30% MeOH and was neutralized to pH 7.0 (1.4 ml 10 N NaOH for 1 liter solvent B). Even though dNTPs are minor components of cell extracts, satisfactory regression coefficients were obtained for their calibration curves (r2 > 0.99) established with the addition–calibration methods up to 120 pmol/40-ml injection. The applicability of the method was demonstrated by in vitro studies of the modulation of NTP and dNTP pools in WiDr colon carcinoma cell lines exposed to various pharmacological concentrations of cytostatic drugs (i.e., FMdC, IUdR, gemcitabine). In conclusion, this optimized, simplified, analytical method enables the simultaneous quantitation of NTP and dNTP and may represent a valuable tool for the detection of minute alterations of cellular dNTP/NTP pools induced by anticancer/ antiviral drugs and diseases. © 1999 Academic Press [less ▲]

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See detailTemperament and character inventory (TCI) and depression.
Hansenne, Michel ULg; Reggers, J.; Pinto, Emmanuel ULg et al

in Journal of Psychiatric Research (1999), 33(1), 31-6

Although several studies have assessed the relationships between the temperament dimensions of the Cloninger model of personality and depression, little is known about the role played by the character ... [more ▼]

Although several studies have assessed the relationships between the temperament dimensions of the Cloninger model of personality and depression, little is known about the role played by the character dimensions proposed by the seven-factor model of Cloninger in depression. In this study, the relationships between the Temperament and Character Inventory (TCI) and depression were examined in a sample of 40 major depressive patients and 40 healthy controls. Depressed patients exhibit higher harm avoidance and self-transcendence scores as well as lower self-directedness and cooperativeness scores as compared to healthy controls. However, the three other dimensions do not differ between depressive patients and controls. Among the depressive group, harm avoidance, self-directedness and cooperativeness dimensions are related to the severity of depression as assessed by the Hamilton scale. This study confirms the state dependence of the harm avoidance dimension and suggests a relationship between the character dimensions of the Cloninger model and depression. [less ▲]

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See detailCorticosensitivity in whole blood cell cultures in Crohn's disease and healthy subjects
Franchimont, Denis; Louis, Edouard ULg; Geenen, Vincent ULg et al

in Digestive Diseases & Sciences (1999), 44

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See detailP300 event-related potential and serotonin-1A activity in depression.
Hansenne, Michel ULg; Ansseau, Marc ULg

in European Psychiatry (1999), 14(3), 143-7

The identification of the brain structures and neurotransmitters responsible for the generation and/or modulation of P300 could lead to important clinical implications. Since serotonin disturbances seem ... [more ▼]

The identification of the brain structures and neurotransmitters responsible for the generation and/or modulation of P300 could lead to important clinical implications. Since serotonin disturbances seem to play a critical role in depression, the aim of the study was to assess the possible relationships between the P300 event-related brain potential and serotonergic activity in depression. The study was conducted among 45 major depressive inpatients, and serotonergic activity was assessed by prolactin (PRL) response to flesinoxan (a 5-HT1A agonist). Results showed a significant negative correlation between P300 amplitude and PRL response to flesinoxan (r = -0.40, P = 0.007 at Cz; r = -0.47, P = 0.001 at Pz). In contrast, both P300 latency and reaction time were not related to endocrine response. This study supports a role for serotonin-1A in the neurobiological modulation of P300 amplitude. [less ▲]

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See detailLes aménagements forestiers en Belgique méridionale (Wallonie) : états des lieux et perspectives
Rondeux, Jacques ULg

in Revue Forestière Française (1999), LI(numéro spécial), 241-246

The forests of French-speaking Belgium cover 530,000 hectares that are evenly distributed as between public and private property and as between deciduous and coniferous populations. Since the 90's, the ... [more ▼]

The forests of French-speaking Belgium cover 530,000 hectares that are evenly distributed as between public and private property and as between deciduous and coniferous populations. Since the 90's, the emphasis of forest policy has been on permanency and multiple-use. Forest plans, particularly in public forests, are largely shaped by production, conservation and protection functions as reflected in complete revisions of the computerised compartment register and implementation of many regulatory provisions. This article reviews existing approaches and describes the main planning methods recommended according to types of stands and on whether a forest is public or private, the latter being far less regulated. [less ▲]

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See detailDelayed massive immune hemolysis mediated by minor ABO incompatibility after allogeneic peripheral blood progenitor cell transplantation.
Salmon, Jean ULg; Michaux, S.; Hermanne, J. P. et al

in Transfusion (1999), 39(8), 824-7

BACKGROUND: Bone marrow transplantation with minor ABO incompatibility may be followed by moderate delayed hemolysis of the recipient's red cells by donor-derived ABO antibodies. This reaction may be more ... [more ▼]

BACKGROUND: Bone marrow transplantation with minor ABO incompatibility may be followed by moderate delayed hemolysis of the recipient's red cells by donor-derived ABO antibodies. This reaction may be more severe after transplantation of peripheral blood progenitor cells (PBPCs). CASE REPORT: A 16-year-old boy underwent an allogeneic PBPC transplant from his HLA-mismatched mother as treatment for acute myeloblastic leukemia that had proved resistant to induction chemotherapy. Transfusion of the unmanipulated PBPCs proceeded without any complication, despite the difference in ABO blood group (donor, O Rh-positive; recipient, A Rh-positive). On Day 7, a rapid drop in hemoglobin to 4 g per dL was observed, which was attributed to a massive hemolysis. All the recipient's group A red cells were destroyed within 36 hours. This delayed and rapidly progressive hemolytic anemia was not associated with the transfusion of the donor's plasma. Rather, the anti-A titer increased in parallel with marrow recovery, which suggested an active synthesis of these antibodies by immunocompetent cells from the donor against the recipient's red cells. The mother's anti-A titer was retrospectively found to be 2048. Her unusually high titer is probably due to prior sensitization during pregnancies. On Day 12, the patient developed grade IV graft-versus-host disease, which proved resistant to all treatments instituted and led to his death on Day 35. CONCLUSION: PBPC transplantation with minor ABO incompatibility may be associated with significant risk of massive delayed hemolysis. [less ▲]

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See detailNonprofit Organisations and Social Economy: Two Ways of Understanding the Third Sector
Mertens de Wilmars, Sybille ULg

in Annals of Public and Cooperative Economics = Annales de l'Economie Publique, Sociale et Coopérative (1999), 70:3

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by ... [more ▼]

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by focusing on the distinction between third sector and state-owned bodies. Next I propose to translate the non capitalist and the non governmental conditions into operational criteria. I show that these criteria vary according to the nature of the organizations (general interest versus mutual interest). This statement allows me to justifiy the emergence of two different ways of understanding the third-sector in anglo-saxon and french-speaking traditions. [less ▲]

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See detailThe value of pretreatment cell kinetic parameters as predictors for radiotherapy outcome in head and neck cancer : a multicenter analysis.
Begg, A-C; Hart, AAM; Dische, S et al

in Radiotherapy & Oncology (1999), 50

Purpose: The aim of this study was to assess the potential of pre-treatment cell kinetic parameters to predict outcome in head and neck cancer patients treated by conventional radiotherapy. Materials and ... [more ▼]

Purpose: The aim of this study was to assess the potential of pre-treatment cell kinetic parameters to predict outcome in head and neck cancer patients treated by conventional radiotherapy. Materials and methods: Data from 11 different centers were pooled. Inclusion criteria were such that the patients received radiotherapy alone, and that the radiotherapy was given in an overall time of at least 6 weeks with a dose of at least 60 Gy. All patients received a tracer dose of either iododeoxyuridine (IdUrd) or bromodeoxyuridine (BrdUrd) intravenously prior to treatment and a tumor biopsy was taken several hours later. The cell kinetic parameters labeling index (LI), DNA synthesis time (Ts) and potential doubling time (Tpot) were subsequently calculated from flow cytometry data, obtained on the biopsies using antibodies against I/BrdUrd incorporated into DNA. Each center carried out their own flow cytometry analysis. Results: From the 11 centers, a total of 476 patients conforming to the inclusion criteria were analyzed. Median values for overall time and total dose were 49 days and 69 Gy, respectively. Fifty one percent of patients had local recurrences and 53% patients had died, the majority from their disease. Median follow-up was 20 months; being 30 months for surviving patients. Multivariate analysis revealed that T-stage, maximum tumor diameter, differentiation grade, N-stage, tumor localization and overall time correlated with locoregional control, in decreasing order of significance. For the cell kinetic parameters, univariate analysis showed that only LI was significantly associated with local control (P=0.02), with higher values correlating with a worse outcome. Ts showed some evidence that patients with longer values did worse, but this was not significant (P=0.06). Tpot showed no trend (P=0.8). When assessing survival in a univariate analysis, neither LI nor Tpot associated with outcome (P=0.4, 0.4, respectively). Surprisingly, Ts did correlate with survival, with longer values being worse (P=0.02). In the multivariate analysis of local control, LI lost its significance (P=0.16). Conclusions: The only pretreatment kinetic parameter for which some evidence was found for an association with local control (the best end-point for testing the present hypothesis) was LI, not Tpot, and this evidence disappeared in a multivariate analysis. It therefore appears that pretreatment cell kinetic measurements carried out using flow cytometry, only provide a relatively weak predictor of outcome after radiotherapy in head and neck cancer. [less ▲]

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See detailVaccinia virus intracellular mature virions contain only one lipid membrane
Hollinshead, M.; Vanderplasschen, Alain ULg; Smith, G. L. et al

in Journal of Virology (1999), 73

Vaccinia virus (VV) morphogenesis commences with the formation of lipid crescents that grow into spherical immature virus (IV) and then infectious intracellular mature virus (IMV) particles. Early studies ... [more ▼]

Vaccinia virus (VV) morphogenesis commences with the formation of lipid crescents that grow into spherical immature virus (IV) and then infectious intracellular mature virus (IMV) particles. Early studies proposed that the lipid crescents were synthesized de novo and matured into IMV particles that contained a single lipid bilayer (S. Dales and E. H. Mosbach, Virology 35:564-583, 1968), but a more recent study reported that the lipid crescent was derived from membranes of the intermediate compartment (IC) and contained a double lipid bilayer (B. Sodiek et al., J. Cell Biol. 121:521-541, 1993). In the present study, we used high-resolution electron microscopy to reinvestigate the structures of the lipid crescents, IV, and IMV particles in order to determine if they contain one or two membranes. Examination of thin sections of Epon-embedded, VV-infected cells by use of a high-angular-tilt series of single sections, serial-section analysis, and high-resolution digital-image analysis detected only a single, 5-nm-thick lipid bilayer in virus crescents, IV, and IMV particles that is covered by a 8-nm-thick protein coat. In contrast, it was possible to discern tightly apposed cellular membranes, each 5 nm thick, in junctions between cells and in the myelin sheath of Schwann cells around neurons. Serial-section analysis and angular tilt analysis of sections detected no continuity between virus lipid crescents or IV particles and cellular membrane cisternae. Moreover, crescents were found to form at sites remote from IC membranesnamely, within the center of virus factories and within the nucleusdemonstrating that crescent formation can occur independently of IC membranes. These data leave unexplained the mechanism of single-membrane formation, but they have important implications with regard to the mechanism of entry of IMV and extracellular enveloped virus into cells; topologically, a one-to-one membrane fusion suffices for delivery of the IMV core into the cytoplasm. Consistent with this, we have demonstrated previously by confocal microscopy that uncoated virus cores within the cytoplasm lack the IMV surface protein D8L, and we show here that intracellular cores lack the surface protein coat and lipid membrane. [less ▲]

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See detailQuantitative determination of haptoglobin (HAP) in human and bovine sera by capillary zone electrophoresis (CZE).
Pirlot, Alain ULg; Janssens, J.; Skinner, G. et al

in Veterinary research (1999), 30(5), 483-93

This study describes an original assay for serum haptoglobin determination by measuring the capacity of human haptoglobin (hHAP) and bovine haptoglobin (bHAP) to bind haemoglobin (Hb) as established by ... [more ▼]

This study describes an original assay for serum haptoglobin determination by measuring the capacity of human haptoglobin (hHAP) and bovine haptoglobin (bHAP) to bind haemoglobin (Hb) as established by capillary zone electrophoresis (CZE). This method involves the addition of Hb in excess to the serum and the separation of the HAP-Hb complexes from free haemoglobin by CZE. Protein migration was recorded at a wavelength of 415 nm which reveals Hb alone (free or bound), and the concentration of HAP was indirectly estimated by measuring bound Hb. Different CZE conditions and the peak migration time of Hb from various species (human, equine, bovine, canine) were investigated. The electrophoretic separation of free human Hb (hHb) in excess and the hHAP-hHb complex was fully achieved by CZE, allowing a quantitative determination of hHAP. However, bovine haemoglobin (bHb) bound to bHAP and free bHb were poorly separated under the same conditions. The best detachment between bHAP-Hb complexes and free Hb was only attained in the bovine sample by use of canine haemoglobin (cHb). CZE assays performed with cHb gave very close values to those of a classic photometric method which measured the peroxidase activity of the haptoglobin-cyanmethaemoglobin complexes (y = 1.0168x - 0.072; r2 = 0.97). CZE assay was fast (< 10 min), inexpensive, did not require the use of a specific antibody and was reproducible (coefficient of variation, CV 3.6%). [less ▲]

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See detailPeripheral blood progenitor cell collections in cancer patients: analysis of factors affecting the yields.
Sautois, Brieuc ULg; Fraipont, V.; Baudoux, Etienne ULg et al

in Haematologica (1999), 84(4), 342-9

BACKGROUND AND OBJECTIVE: Peripheral blood progenitor cells (PBPC) are now widely used to restore hematopoiesis following high dose chemotherapy in patients with malignancies. We sought to identify ... [more ▼]

BACKGROUND AND OBJECTIVE: Peripheral blood progenitor cells (PBPC) are now widely used to restore hematopoiesis following high dose chemotherapy in patients with malignancies. We sought to identify parameters that could predict the yield of PBPC after mobilization with chemotherapy (CT) with or without granulocyte colony-stimulating factor (G-CSF) in cancer patients. DESIGN AND METHODS: One hundred and fifty patients underwent 627 PBPC collections during the recovery phase following CT with (n = 469) or without (n = 142) G-CSF. Hemogram, CFC-assays and CD34+ cell count were performed on peripheral blood and leukaphereses products. After log transformation of the data, differences between groups were assessed with the unpaired t-test or one-way analysis of variance. RESULTS: Seventeen and two patients required 2 and 3 mobilization cycles respectively to reach our target of 15x10(4) CFU-GM/kg. In patients with lymphoma but not in those with leukemia, the yields of both CFU-GM and CD34+ cells/kg were dramatically increased when G-CSF was added to CT for mobilization. In collections primed with CT and G-CSF, better yields were obtained in patients with breast cancer or small-cell lung carcinoma (SCLC) as opposed to other solid tumors and leukemia. Among potential predictive factors of CT- and G-CSF-primed harvests, we found that the CD34+ cell count in peripheral blood (PB) was strongly correlated with both the CFU-GM and CD34+ cell yields. Except in leukemia patients, more than 1x10(6) CD34+ cells/kg were harvested when the CD34+ cell count in blood was above 20x10(6)/L. Similarly, better results were obtained in collections performed when the percentage of myeloid progenitors in blood on the day of apheresis was above 5 % or when the leukocyte count in blood was above 5x10(9)/L. INTERPRETATION AND CONCLUSIONS: A diagnosis of breast cancer or SCLC, a leukocyte count in PB of more than 5x10(9)/L, more than 5% myeloid progenitors or more than 20x10(6) CD34+ cells/L in PB were associated with higher yields of PBPC in collections mobilized with CT+G-CSF. [less ▲]

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See detailScintigraphy of the equine lung
Votion, Dominique ULg; Lekeux, Pierre ULg

in Equine Veterinary Education (1999), 11

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See detailEffects of glucagon in the control of endogenous glucose production in man.
Surmely, J. F.; Schneiter, P.; Henry, S. et al

in Nutrition (1999), 15(4), 267-73

Endogenous glucose production has been shown to increase during administration of glucagon + fructose, but not during administration of fructose alone. To determine the mechanisms by which glucagon exerts ... [more ▼]

Endogenous glucose production has been shown to increase during administration of glucagon + fructose, but not during administration of fructose alone. To determine the mechanisms by which glucagon exerts this action, endogenous glucose production (EGP) and gluconeogenesis from fructose (GNF) were measured in eight healthy subjects infused 1) with graded doses of glucagon (2 and 4 ng.kg-1.min-1 for 3 h each) during constant infusion of 13C-fructose (3 mg.kg-1.min-1), and 2) with graded doses of 13C-fructose (3 and 6 mg.kg-1.min-1) during constant glucagon infusion (2 ng.kg-1.min-1). GNF was estimated from 13C-glucose synthesis. In both protocols, infusion of 3 mg.kg-1.min-1 fructose + 2 ng.kg-1.min-1 glucagon increased EGP by 5-8% (P < 0.05), while GNF represented 43-49% of EGP. Thereafter, increasing the glucagon infusion rate further increased EGP to 118 +/- 3% of basal values (P < 0.01) without altering the proportion due to GNF. In contrast, increasing the fructose infusion rate at constant glucagonemia increased EGP similarly (by 19 +/- 4%, P < 0.05) but enhanced the contribution of GNF to 76 +/- 2% (P < 0.001). Graded infusion of glucagon or fructose alone failed to stimulate EGP. The present findings indicate that hyperglucagonemia stimulates endogenous glucose production during fructose infusion. This effect is not secondary to a stimulation of gluconeogenesis, but to a channelling of glucose-6-phosphate towards systemic release. [less ▲]

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See detailStructure and functional analysis of a tilapia (Oreochromis mossambicus) growth hormone gene: activation and repression by pituitary transcription factor Pit-1
Sekkali, Belaid; Brim, Hassan; Muller, Marc ULg et al

in DNA & Cell Biology (1999), 18(6), 489-502

A gene encoding the Tilapia mossambica (Oreochromis mossambicus) growth hormone (tiGH) was isolated and sequenced. The gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences and a 1 ... [more ▼]

A gene encoding the Tilapia mossambica (Oreochromis mossambicus) growth hormone (tiGH) was isolated and sequenced. The gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences and a 1.7-kb transcription unit comprised of six exons and five introns. The gene and the 5' flanking region contain several potential binding sites for Pit-1, a key transcription activator of mammalian GH genes. One of these (-57/-42) is highly conserved in fish GH genes. It activates transcription in pituitary cells and binds Pit-1. Transfection of luciferase reporter plasmids containing either the -3602/+19 tiGH sequence or one of its 5' deletion mutants (-2863/, -1292/, and -463/+19) resulted in strong activity in Pit-1-producing rat pituitary GC cells. A dose-dependent activation of the tiGH promoter was achieved in nonpituitary fish EPC and monkey COS cells cotransfected with a rat Pit-1 expression vector, demonstrating the crucial role played by Pit-1 as an activator of the tiGH gene. Fusion of the tiGH promoter with the beta-galactosidase gene led to transient expression specifically in the nervous system of microinjected zebrafish embryos. The activity of the tiGH promoter in GC and EPC cells was strongly repressed by extending its 3' end from +19 to +40, a sequence in which a Pit-1-binding site was identified using gel retardation assays. Point mutations of the site that suppressed Pit-1 binding in vitro restored full tiGH promoter activity. Thus, a Pit-1-binding site located in the 5' untranslated region mediates Pit-1-dependent repression of the tiGH gene. [less ▲]

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See detailEstimates of the annual net carbon and water exchange of forests: the EUROFLUX methodology.
Aubinet, Marc ULg; Grelle, A.; Ibrom, A. et al

in Advances in Ecological Research (1999), 30

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See detailP300 and personality: an investigation with the Cloninger's model.
Hansenne, Michel ULg

in Biological Psychology (1999), 50(2), 143-55

The relationships between P300 and personality have been explored mainly in reference to the model of personality described by Eysenck because of its biological bases. Recently, Cloninger and his ... [more ▼]

The relationships between P300 and personality have been explored mainly in reference to the model of personality described by Eysenck because of its biological bases. Recently, Cloninger and his colleagues have proposed a model of personality based on four temperaments and three characters. The Temperament and Character Inventory (TCI) is a 226-item self-questionnaire developed to assess these seven dimensions of personality. In the present study, the relationships between these dimensions of personality and P300 have been investigated in 43 normal subjects. The results show that P300 amplitude is positively correlated with the novelty seeking dimension and negatively correlated with the harm avoidance dimension. In contrast, the other dimensions of the TCI were not related to P300 amplitude. Moreover, P300 latency and reaction time were not associated with the TCI dimensions of personality. This study confirms that personality is related to P300. [less ▲]

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See detailExploration isocinétique : analyse des paramètres chiffrés
Croisier, Jean-Louis ULg; Crielaard, Jean-Michel ULg

in Annales de Réadaptation et de Médecine Physique (1999), 42

Introduction : Dans le domaine de l'évaluation de la fonction musculaire, l'isocinétisme permet la quantification de paramètres définissant la qualité de force. Méthode-Objectif : Il s'agit cependant d ... [more ▼]

Introduction : Dans le domaine de l'évaluation de la fonction musculaire, l'isocinétisme permet la quantification de paramètres définissant la qualité de force. Méthode-Objectif : Il s'agit cependant d'optimaliser l'interprétation du bilan isocinétique. Le choix des paramètres les plus représentatifs exige une étude de reproductibilité. Résultats : Lors d'efforts concentriques et excentriques des muscles fléchisseurs et extenseurs du genou, le moment de force maximum répond aux critères qualitatifs souhaités (coefficients de variation faibles); les paramètres de puissance et de travail ne semblent pas apporter d'information complémentaire discriminante en pratique courante. Conclusion: Les auteurs proposent des valeurs de référence et fixent des "limites" précises permettant de distinguer le normal du pathologique, particulièrement pour les paramètres différence bilatérale et rapport agonistes/antagonistes. [less ▲]

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See detailChanges in mediobasal hypothalamic gonadotropin-releasing hormone messenger ribonucleic acid levels induced by mating or ovariectomy in a reflex ovulator, the ferret.
Bakker, Julie ULg; Rubin, B. S.; Baum, M. J.

in Endocrinology (1999), 140(2), 595-602

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large ... [more ▼]

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large release of GnRH from the mediobasal hypothalamus (MBH). In Exp 1 we asked whether GnRH messenger RNA (mRNA) levels increase in response to mating so as to replenish the MBH GnRH stores needed to sustain the preovulatory LH surge. Estrous females were killed 0, 0.25, 0.5, 1, 3, 6, 14, or 24 h after the onset of a 10-min intromission from a male. Coronal brain sections ranging from the rostral preoptic area caudally to the posterior hypothalamus were processed for in situ hybridization using a 35S-labeled oligoprobe complementary to the human GnRH-coding region. We found no evidence of increased MBH GnRH mRNA levels during the ferret's mating-induced preovulatory LH surge. Instead, the number of GnRH mRNA-expressing cells dropped significantly in the arcuate region beginning 6 h after onset of intromission and remained low thereafter. Furthermore, cellular GnRH mRNA levels decreased in the arcuate region toward the end of the preovulatory LH surge. In Exp 2 we asked whether ovarian hormones regulate MBH GnRH mRNA levels in the female ferret. Ovariectomy of estrous females significantly reduced the number of GnRH mRNA-expressing cells in the arcuate region. This decrease was probably not due to the absence of circulating estradiol. Gonadally intact anestrous females had levels of MBH GnRH mRNA similar to those in estrous females even though plasma estradiol levels were equally low in anestrous females and ovariectomized females. Ovarian hormones other than estradiol may stimulate MBH GnRH mRNA levels in anestrous and estrous females. [less ▲]

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See detailStades et supporters: De la violence à la prévention des dérives passionnelles
Stassen, Benjamin; Comeron, Manuel ULg

in Revue Sport (1999), (165-166), 128

Analyse des phénomènes de violences et déviances des supporters de football et stratégies de prévention du hooliganisme par D. Bodin (Rennes), M. Comeron (Liège), J. Jacquemart (Liège), E. Jareno ... [more ▼]

Analyse des phénomènes de violences et déviances des supporters de football et stratégies de prévention du hooliganisme par D. Bodin (Rennes), M. Comeron (Liège), J. Jacquemart (Liège), E. Jareno (Barcelone), G. Kellens (Liège), F. Lovenfosse (Liège), M. Marcus (Paris), C. Moneypenny (Liverpool), Piastowski (Düsseldorf) [less ▲]

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See detailUn siècle d'histoire coopérative à travers les statistiques de l'A.C.I.
Mignot, Delphine; Defourny, Jacques ULg; Leclerc, André

in Annals of Public and Cooperative Economics = Annales de l'Economie Publique, Sociale et Coopérative (1999), 70(1), 75-105

Beaucoup d'auteurs se sont déjà penchés sur l'évolution quantitative du mouvement coopératif; mais généralement leurs analyses se limitaient à un secteur, à une région ou à une période particulière. Grâce ... [more ▼]

Beaucoup d'auteurs se sont déjà penchés sur l'évolution quantitative du mouvement coopératif; mais généralement leurs analyses se limitaient à un secteur, à une région ou à une période particulière. Grâce à une collecte effectuée par A. Leclerc (Université de Moncton, Canada) de toutes les informations statistiques détenues par l'Alliance Coopérative Internationale, ce travail peut s'appuyer sur une base de données exceptionnelle concernant le nombre de coopérateurs affiliés à l'A.C.I. depuis 1896. Sur base de ce recensement, les auteurs ont cherché à déterminer les grandes orientations de l'évolution du sociétariat coopératif sur une période longue de près d'un siècle. Dans cet article, ils présentent la plupart des résultats obtenus en découpant la période analysée en quatre phases de développement du mouvement coopératif mondial. [less ▲]

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See detailGlobal stabilization of feedforward systems with exponentially unstable Jacobian linearization
Grognard, F.; Sepulchre, Rodolphe ULg; Bastin, G.

in Systems & Control Letters (1999), 37(2), 107-115

The global stabilization of a class of feedforward systems having an exponentially unstable Jacobian linearization is achieved by a high gain feedback saturated at a low level. The control law forces the ... [more ▼]

The global stabilization of a class of feedforward systems having an exponentially unstable Jacobian linearization is achieved by a high gain feedback saturated at a low level. The control law forces the derivatives of the state variables to small values along the closed loop trajectories. This “slow control” design is illustrated with a benchmark example and its limitations are emphasized. [less ▲]

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See detailIn vitro root cultures of Panax ginseng and P-quinquefolium
Kevers, Claire ULg; Jacques, Philippe; Thonart, Philippe ULg et al

in Plant Growth Regulation (1999), 27(3), 173-178

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took ... [more ▼]

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots. [less ▲]

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See detailNew initiator system for the anionic polymerization of (meth)acrylates in toluene. IV. Random copolymerization of (meth)acrylates in toluene initiated by s-Buli ligated by lithium silanolates
Zune, Catherine; Zundel, Thomas; Dubois, Philippe ULg et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1999), 37(14), 2525-2535

Copolymerization of binary mixtures of alkyl (meth)acrylates has been initiated in toluene by a mixed complex of lithium silanolate (s-BuMe2SiOLi) and s-BuLi (molar ratio > 21) formed in situ by reaction ... [more ▼]

Copolymerization of binary mixtures of alkyl (meth)acrylates has been initiated in toluene by a mixed complex of lithium silanolate (s-BuMe2SiOLi) and s-BuLi (molar ratio > 21) formed in situ by reaction of s-BuLi with hexamethylcyclotrisiloxane (D3). Fully acrylate and methacrylate copolymers, i.e., poly(methyl acrylate-co-n-butyl acrylate), poly(methyl methacrylate-co-ethyl methacrylate), poly(methyl methacrylate-co-n-butyl methacrylate), poly(methyl methacrylate-co-n-butyl methacrylate), poly(isobornyl methacrylate-co-n-butyl methacrylate), poly(isobornyl methacrylate-co-n-butyl methacrylate) of a rather narrow molecular weight distribution have been synthesized. However, copolymerization of alkyl acrylate and methyl methacrylate pairs has completely failed, leading to the selective formation of homopoly(acrylate). As result of the isotactic stereoregulation of the alkyl methacrylate polymerization by the s-BuLi/s-BuMe2SiOLi initiator, highly isotactic random and block copolymers of (alkyl) methacrylates have been prepared and their thermal behavior analyzed. The structure of isotactic poly(ethyl methacrylate-co-methyl methacrylate) copolymers has been analyzed in more detail by Nuclear Magnetic Resonance (NMR). [less ▲]

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See detailCAPPP trial. Captopril Prevention Project.
Scheen, André ULg

in Lancet (1999), 353(9166), 1793-41795-6

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See detailLe zona chez l'enfant
Nikkels, Arjen ULg; Tassoudji, Nazli ULg; Pierard, Gérald ULg

in Skin (1999), 2(1), 7-8

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See detailLa mora debitoris est-elle une faute?
Gerkens, Jean-François ULg

in Mélanges offerts en l'Honneur de Fritz Sturm (1999), I

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See detailDevelopment of a monoclonal antibody-based sandwich ELISA for the détection of bovine enteropathogenic and enterohaemorrhagic strains of Escherichia coli O26
Kerr, P.; China, B.; Mainil, Jacques ULg et al

in Clinical and Diagnostic Laboratory Immunology (1999), 6

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See detailDissections artérielles et manipulations cervicales
DELVAUX, Valérie ULg; GERARD, Pascale ULg; DE PASQUA, Victor ULg et al

in Revue Neurologique (1999), 155(Supp 1), 160

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See detailNutritional analyses for proteins and amino acids in beans (Phaseolus sp.)
Wathelet, Bernard ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(4),

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See detailThe long-term cytoskeletal rearrangement induced by rabbit enteropathogenic Escherichia coli is Esp-dependent but intimin-independent
Nougayrède, J. P.; Marchès, O.; Boury, M. et al

in Molecular Microbiology (1999), 31

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See detailA treatment planning intercomparison of proton and intensity modulated photon radiotherapy.
Lomax, T; Goitein, G; Debus, J et al

in Radiotherapy & Oncology (1999), 51(3)

Abstract Purpose: A comparative treatment planning study has been undertaken between standard photon delivery techniques,b intensity modulated photon methods and spot scanned protons in order to ... [more ▼]

Abstract Purpose: A comparative treatment planning study has been undertaken between standard photon delivery techniques,b intensity modulated photon methods and spot scanned protons in order to investigate the merits and limitations of each of these treatment approaches. Methods: Plans for each modality were performed using CT scans and planning information for nine patients with varying indications and lesion sites and the results have been analysed using a variety of dose and volume based parameters. Results: Over all cases, it is predicted that the use of protons could lead to a reduction of the total integral dose by a factor three compared to standard photon techniques and a factor two compared to IM photon plans. In addition, in all but one Organ at Risk (OAR) for one case, protons are predicted to reduce both mean OAR dose and the irradiated volume at the 50% mean target dose level compared to both photon methods. However, when considering the volume of an OAR irradiated to 70% or more of the target dose, little difference could be shown between proton and intensity modulated photon plans. On comparing the magnitude of dose hot spots in OARs resulting from the proton and IM photon plans, more variation was observed, and the ranking of the plans was then found to be case and OAR dependent. Conclusions: The use of protons has been found to reduce the medium to low dose load (below about 70% of the target dose) to OARs and all non-target tissues compared to both standard and inversely planned photons, but that the use of intensity modulated photons can result in similar levels of high dose conformation to that afforded by protons. However, the introduction of inverse planning methods for protons is necessary before general conclusions on the relative efficacy of photons and protons can be drawn. [less ▲]

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See detailA novel approach to the study of familial influences on evoked cortical responses in migraine.
Schoenen, Jean ULg; Sandor, P. S.

in Cephalalgia : An International Journal of Headache (1999), 19 Suppl 24

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See detailEtude du role de Clostridium perfringens dans l'entérotoxémie bovine
Manteca, Christophe; Daube, Georges ULg; Mainil, Jacques ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(6, Pt 2),

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as ... [more ▼]

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as clinical signs, if any. The most pronounced lesion is a necrohaemorrhagic enteritis of the jejunum, the ileum, and sometimes the colon. Suckling beef calves are the most frequently affected ones. In 67% of the 78 field cases investigated, some kind of stress was observed 24 to 36 hours prior to the death: change in diet or pasture, vaccination... The most frequently isolated bacteria, and the one isolated in highest numbers, was non-sporulated non-enterotoxigenic toxinotype A Clostridium perfringens. Reproduction of the lesions was successful in a ligated intestinal loop assay in one calf with a few of these strains, more especially with one of them, which was shown later to produce another recently described toxin, the beta 2 toxin. A role for this beta 2 toxin in bovine enterotoxaemia is thus speculated for future research. [less ▲]

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See detailDo babies resemble their fathers more than their mothers ? : a failure to replicate Christenfeld and Hill
Brédart, Serge ULg; French, R.

in Evolution and Human Behavior : Official Journal of the Human Behavior and Evolution Society (1999), 20(3), 129-135

Contrary to Christenfeld

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See detailFluorodopa uptake and glucose metabolism in early stages of corticobasal degeneration.
Laureys, Steven ULg; Salmon, Eric ULg; Garraux, Gaëtan ULg et al

in Journal of Neurology (1999), 246(12), 1151-8

Fluorodopa (FDOPA) and fluorodeoxyglucose (FDG) PET was performed in six patients in early stages of corticobasal degeneration (CBD) and compared to Parkinson's disease (PD) patients with a similar degree ... [more ▼]

Fluorodopa (FDOPA) and fluorodeoxyglucose (FDG) PET was performed in six patients in early stages of corticobasal degeneration (CBD) and compared to Parkinson's disease (PD) patients with a similar degree of bradykinesia and rigidity and to healthy controls. Statistical parametric mapping analysis comparing CBD to controls showed metabolic decrease in premotor, primary motor, supplementary motor, primary sensory, prefrontal, and parietal associative cortices, and in caudate and thalamus contralateral to the side of clinical signs. Except for the prefrontal regions a similar metabolic pattern was observed when CBD was compared to PD. Putamen FDOPA uptake was decreased in both CBD and PD. Caudate FDOPA uptake in CBD patients was decreased contralateral to clinical signs when compared to controls, but was higher than in PD. In early stages of CBD, FDOPA and FDG PET patterns differed from those observed in PD. In CBD the asymmetry in FDOPA uptake was less pronounced than that of clinical signs or metabolic impairment. [less ▲]

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See detailCalcul et conception économique des assemblages de profilés reconstitués soudés
Braham, M.; Cerfontaine, F.; Jaspart, Jean-Pierre ULg

in Construction Métallique (1999), (N°1), 5-24

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See detailLe nouvel article 867 du Code judiciaire
Boularbah, Hakim ULg

in Journal des Tribunaux (1999)

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See detailPlatyhypnidium mutatum, a mysterious new moss from Germany
Ochyra, R.; Vanderpoorten, Alain ULg

in Journal of Bryology (1999), 21

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See detailThe Solvation Concept Approached By Lipophily
Van Eyck, Mh.; Ducarme, P.; Benhabiles, N. et al

in Analusis (1999), 27(1), 6-14

The soivent of biological media is water and interactions between water and solutes are major to explain membrane and protein structures, Therefore, mimicking solvation effects is a challenge to compute ... [more ▼]

The soivent of biological media is water and interactions between water and solutes are major to explain membrane and protein structures, Therefore, mimicking solvation effects is a challenge to compute native structures of biological molecules. Lipophlly is an, experimental approach of solvation sinca it measures the parItion of a solute between two solvents, the reterence being water. In the last decade, different attempts were made to extract parameters from lipophily that will enable to describe soivation of complex molecules. We discuss here some analysis made with the atomie transfer energy and the atomic aurface parameter in the study of protein folding and protein Insertion in membranes. [less ▲]

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See detailDélégation par abandon ; approche psychologique
Born, Michel ULg

in Les politiques sociales (1999), 3-4

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See detailChemical changes and influences of rapeseed antinutritional factors on lamb physiology and performance - 1. Animal performance and thyroid histology
Derycke, G.; Mabon, N.; Mandiki, S. N. M. et al

in Animal Feed Science & Technology (1999), 81(1-2), 81-91

Sixty six Texel, Suffolk or crossbred lambs allocated to three groups were used. From the end of their first month of age till slaughtering, they were fed ad libitum with concentrates containing 0 ... [more ▼]

Sixty six Texel, Suffolk or crossbred lambs allocated to three groups were used. From the end of their first month of age till slaughtering, they were fed ad libitum with concentrates containing 0% (Control) or 25% of rapeseed meal obtained from either Samourai (Samourai) or Honk (Honk) variety. The Samourai and Honk concentrates contained 1.95 and 4.22 mmoles/g DM of glucosinolates, respectively. Lambs were weaned at 88 +/- 8 days of age and 24 +/- 5 kg of live weight and were slaughtered when their fattening state was estimated to be optimal. Apart from low concentrate intake for suckling lambs at the beginning of the experiment in the Honk, no negative effect of low glucosinolates (LG)-rapeseed meal was noted concerning animal performance. The quality of perirenal fat was better for lambs which received rapeseed meal since lower (P < 0.5) proportions of saturated fatty acids (C-10 : 0, C-12 : 0, C-14 : 0) and higher contents (P < 0.05) of some unsaturated fatty acids (C-18 : 1trans, C-22 : 2) were determined. The thyroid weight was significantly higher (P < 0.05) for the Honk (3.04 +/- 0.8 g) than in the Control (2.6 +/- 0.5 g) and the Samourai (2.7 +/- 0.6 g). Moreover, the percentages of small thyroid follicles were lower (P < 0.05) in the Honk than in the Control and Samourai groups. Conversely, the percentages of large thyroid follicles were higher (P < 0.05) in the Honk than in the two other groups. These results suggest that rapeseed meal can be used as the only protein supplement for fattening lambs despite the modification of thyroid histology by high glucosinolate content. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailCandidate gene markers associated with somatotropic axis and milk selection
Parmentier, I.; Portetelle, Daniel ULg; Gengler, Nicolas ULg et al

in Domestic Animal Endocrinology (1999), 17(2-3), 139-148

One of the obstacles to progress in dairy cattle selection is that milk production traits are only expressed after the first calving. However, the use of the quantitative trait loci (QTL) technology will ... [more ▼]

One of the obstacles to progress in dairy cattle selection is that milk production traits are only expressed after the first calving. However, the use of the quantitative trait loci (QTL) technology will improve the efficiency of dairy industry with a positive image for the consumers. QTL are part of the genome showing a preponderant action and explaining the major part of variation of the trait production. At the present time, the two major strategies developed to detect such QTL are the candidate gene approach and the positional genetics approach. The somatotropic axis contains the most promising candidates in this respect, as it strongly regulates milk production. Then, the identification of favorable QTL associated with the somatotropic axis that are significantly correlated with genetic merits for milk production could lead to more effective selection programs. (C) 1999 Elsevier Science Inc. All rights reserved. [less ▲]

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See detailContribution of the carbohydrate moiety to conformational stability of the carboxypeptidase Y high pressure study.
Dumoulin, Mireille ULg; Ueno, H.; Hayashi, R. et al

in European Journal of Biochemistry (1999), 262(2), 475-83

The process of pressure-induced denaturation of carboxypeptidase Y and the role of the carbohydrate moiety in its response to pressure and low temperature were investigated by measuring in situ the ... [more ▼]

The process of pressure-induced denaturation of carboxypeptidase Y and the role of the carbohydrate moiety in its response to pressure and low temperature were investigated by measuring in situ the catalytic activity and, the intrinsic and 8-anilino-1-naphthalene sulfonic acid binding fluorescences. Pressure-induced denaturation of carboxypeptidase Y is a process involving at least three transitions. Low pressures (below 150 MPa) induced slight conformational changes characterized by a slight decrease in the center of the spectral mass of intrinsic fluorescence, whereas no changes in 8-anilino-1-naphthalene sulfonic acid binding fluorescence were observed and 80% of the catalytic activity remained. Higher pressure (150-500 MPa) induced further conformational changes, characterized by a large decrease in the center of the spectral mass of intrinsic fluorescence, a large increase in the 8-anilino-1-naphthalene sulfonic acid binding fluorescence and the loss of all catalytic activity. Thus, this intermediate exhibited characteristics of molten globule-like state. A further increase, in pressure (above 550 MPa) induced transition from this first molten globule-like state to a second molten globule-like state. This two-stage denaturation process can be explained by assuming the existence of two independent structural domains in the carboxypeptidase molecule. A similar three-transition process was found for unglycosylated carboxypeptidase Y, but, the first two transitions clearly occurred at lower pressures than those for glycosylated carboxypeptidase Y. These findings indicate that the carbohydrate moiety protects carboxypeptidase Y against pressure-induced denaturation. The origin of the protective effects is discussed based on the known crystallographic structure of CPY. [less ▲]

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See detailUse of multitrait evaluation procedures to improve reliability of early prediction of survival
Druet, Tom ULg; Solkner, J.; Gengler, Nicolas ULg

in Journal of Dairy Science (1999), 82(9), 20541-205416

Multitrait, across-country evaluation procedures were adapted to improve reliability of survival breeding values by combining direct information with yield and functional traits: milk persistency, somatic ... [more ▼]

Multitrait, across-country evaluation procedures were adapted to improve reliability of survival breeding values by combining direct information with yield and functional traits: milk persistency, somatic cell count, fertility (male and female), and calving ease (direct and maternal). A set of bulls was selected from the Austrian Simmental population based on mean original reliabilities of at least 0.50 for yield traits or of at least 0.20 for functional traits. Only breeding values above these limits were retained. The breeding values were deregressed, assuming that they were obtained by single-trait, sire-maternal grandsire models. An expectation maximization restricted maximum likelihood algorithm based on the multitrait, across-country evaluation equations was used to compute genetic correlations among all of these traits. These equations were solved, and the reliabilities of the solutions were also estimated. Mean rank correlation between direct and combined breeding values was 0.85 with values as low as 0.67 for the group of youngest bulls, Direct (original), indirect, and combined reliabilities were compared to appreciate the impact of our procedures on improvement of reliability of survival breeding values; This improvement, dependent on the level of reliability of direct and indirect information, could be up to 0.24 for animals with low direct reliability and high indirect reliability. For young bulls born in 1992 without reliable direct survival information but with already reliable information on yield and functional traits, mean reliability improvement was 0.13. For all bulls this value was still 0.06. [less ▲]

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See detailCalcitonine dans la prévention secondaire et tertiaire de l’ostéoporose
Halkin, V; Reginster, Jean-Yves ULg

in Medi-Sphere (1999), 3

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See detailTransplantation combinée du foie et du coeur chez un patient souffrant de thalassémie majeure
Detry, Olivier ULg; Defechereux, Thierry ULg; Honore, Pierre ULg et al

in Médecine & Chirurgie Digestives (1999), 28(3), 109-110

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See detailSensitizing human cervical cancer cells in vitro to ionizing radiation with interferon β or γ
Grueninger, L; Cottin, Y-X; Li, Y-X et al

in Radiation Research (1999), 152(5),

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See detailIdentification Of Mitochondrial Plasmid Like Dnas In Picea Abies (L.) Karst.
Chandelier, A.; Du Jardin, Patrick ULg; Avril, C. et al

in Plant Cell Reports (1999), 18(10),

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See detailBovine Leukemia Virus-Induced Persistent Lymphocytosis In Cattle Does Not Correlate With Increased Ex Vivo Survival Of B Lymphocytes
Dequiedt, Franck ULg; Cantor, Gh.; Hamilton, Vt. et al

in Journal of Virology (1999), 73(2),

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See detailEpidémiosurveillance de la rage animale en Belgique : un seul cas détecté en 1998
Brochier, B.; Dechamps, P.; Costy, F. et al

in Annales de Médecine Vétérinaire (1999), 143

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See detailNonresponsive Generalized Bacterial Infection Associated with Systemic Lupus Erythematosus in a Beauceron
Clercx, Cécile ULg; Mc Entee, Kathleen ULg; Gilbert, S. et al

in Journal of the American Animal Hospital Association (1999), 35(3, May-Jun), 220222224-8

A case of concurrent canine systemic lupus erythematosus (SLE) and generalized bacterial infection in a six-year-old female Beauceron is reported. The dog presented with purulent nasal and ocular ... [more ▼]

A case of concurrent canine systemic lupus erythematosus (SLE) and generalized bacterial infection in a six-year-old female Beauceron is reported. The dog presented with purulent nasal and ocular discharges, skin lesions (including seborrhea, hyperkeratotic areas, and papules as well as ecchymoses around the eyes, on both sides of the pinnae, and on the vulva), generalized lymph node enlargement, a mitral murmur, and lameness. Later, facial swelling, a retrobulbar abscess, and a cough also developed. Occurrence of a generalized bacterial infection was established by culture of group-C, beta-hemolytic Streptococcus from the throat, the mouth, a biopsy site (popliteal lymph node area), the retrobulbar abscess, and the lung. The diagnosis of SLE was based on the clinical signs and particularly on the occurrence of antinuclear antibody (ANA) and antidoublestranded-desoxyribonucleic acid (ds-DNA) antibody. Interestingly, the latter type of antibodies were also detected in two young female puppies whelped by this dog. Salient histological findings included an extreme cell depletion of the lymph nodes and spleen and severe pneumonitis and peribronchiolitis. The results of this case indicate that a definite diagnosis of canine SLE can, at times, be made on the basis of the presence of serum ANA and ds-DNA antibodies. [less ▲]

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See detailObzor verkhnego paleolita Moldavii
Otte, Marcel ULg; Noiret, Pierre ULg; López Bayón, Ignacio

in Stratum Plus (1999), 1

The existing in Moldova Aurignacian industries of the lamellar type are represented by the stone collections of two kinds: with leaf-shaped points and backed points with a concave edge. Together with this ... [more ▼]

The existing in Moldova Aurignacian industries of the lamellar type are represented by the stone collections of two kinds: with leaf-shaped points and backed points with a concave edge. Together with this the Corpach IV industry which includes backed points (segments) must have been related to Gravette. Other industries should not be considered separately and apparently can be grouped under the title "industries de type Paléolithique supérieur ancien à pointes foliacées". They are supposed to spring from the Middle Palaeolithic industries with leaf-shaped pieces (from Micoquian of Central Europe), that were further developed in the Aurignacian direction. If the Aurignacian of Moldova has evidently a foreign origin, then the Gravette traditions go back to some related centers. It is reflected in the compatibilty of backed pieces and leaf-shaped points in one and the same industry, dated back to the early stage of the Upper Palaeolithic. It is clearly seen in the collections of lower layers of Molodova V where homogeneousness of the complex is evident starting with the tenth layer. The appearance of some new types of tools (for instance, shouldered points) in the upper layers of this site does not fail the general stability and the cultural originality of the industry. [less ▲]

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See detailFractal Quantification of the Microvasculature Heterogeneity in Cutaneous Melanoma
Heymans, O.; Blacher, Silvia ULg; Brouers, François ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1999), 198(2), 212-7

BACKGROUND: Recent advances in angiogenesis research have led to the introduction of new prognosis factors. Although the vessel count is effective in breast cancer, the prognosis of primary cutaneous ... [more ▼]

BACKGROUND: Recent advances in angiogenesis research have led to the introduction of new prognosis factors. Although the vessel count is effective in breast cancer, the prognosis of primary cutaneous melanoma (PCM) does not seems to be directly influenced by this parameter. However, the microscopic examination of PCM suggests variability in the repartition of the microvasculature. OBJECTIVE AND METHOD: To explore the possibility of extracting information about the vessel distribution by performing a textural analysis on the grey level of histological sections by means of fractal characterization by both Fourier spectrum and multifractal analysis. RESULTS: Three different patterns of vasculature were identified according to the vessel density and distribution. CONCLUSION: It is possible to differentiate and quantify clearly the differences in the microvessel profile organization using the fractal and multifractal methods. [less ▲]

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See detailA new combined bodian-luxol technique for staining unmyelinated axons in semithin, resin-embedded peripheral nerves: a comparison with electron microscopy.
Deprez, Manuel ULg; Ceuterick-de Groote, C.; Fumal, Arnaud ULg et al

in Acta Neuropathologica (1999), 98(4), 323-9

Quantitation of unmyelinated fibers (UF) in peripheral nerves has classically relied upon ultrastructural morphometry. Because this method is time-consuming, it is not typically performed in routine ... [more ▼]

Quantitation of unmyelinated fibers (UF) in peripheral nerves has classically relied upon ultrastructural morphometry. Because this method is time-consuming, it is not typically performed in routine analysis of nerve biopsies. We applied the Bodian-Luxol technique to detect unmyelinated axons by light microscopy on semithin sections from resin-embedded nerve tissue. Estimates were compared to ultrastructural counts. The staining appeared highly specific for axons. Excellent correlation was found between optic densities and the population of UF larger than 0.5 microm. The smallest profiles detected by light microscopy had a diameter close to 0.6 microm. This new technique is not a substitute for ultrastructural quantitative morphometry of UF, as very small unmyelinated axons, especially regenerating ones, can not be reliably visualized. However, it provides a valuable light microscopic method for evaluating axonal loss among UF. [less ▲]

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See detailAltered apoptotic profiles in irradiated patients with increased toxicity.
Crompton, Nigel; Miralbell, Raymond; Rutz, Hans-Peter et al

in International Journal of Radiation, Oncology, Biology, Physics (1999), 45(3), 707-714

Purpose: A retrospective study of radiation-induced apoptosis in CD4 and CD8 T-lymphocytes, from 12 cancer patients who displayed enhanced toxicity to radiation therapy and 9 ataxia telangiectasia ... [more ▼]

Purpose: A retrospective study of radiation-induced apoptosis in CD4 and CD8 T-lymphocytes, from 12 cancer patients who displayed enhanced toxicity to radiation therapy and 9 ataxia telangiectasia patients, was performed to test for altered response compared to healthy blood-donors and normal cancer patients. Methods and Materials: Three milliliters of heparinized blood from each donor was sent via express post to the Paul Scherrer Institute (PSI) for subsequent examination. The blood was diluted 1:10 in RPMI medium, irradiated with 0-, 2-, or 9-Gy X-rays, and incubated for 48 h. CD4 and CD8 T-lymphocytes were then labeled using FITC-conjugated antibodies, erythrocytes were lysed, and the DNA stained with propidium iodide. Subsequently, cells were analyzed using a Becton Dickinson FACScan flow cytometer. Radiation-induced apoptosis was recognized in leukocytes as reduced DNA content attributed to apoptosis-associated changes in chromatin structure. Apoptosis was confirmed by light microscopy, electron microscopy, and by the use of commercially available apoptosis detection kits (in situ nick translation and Annexin V). Data from hypersensitive individuals were compared to a standard database of 105 healthy blood-donors, and a database of 48 cancer patient blood donors who displayed normal toxicity to radiation therapy. To integrate radiosensitivity results from CD4 and CD8 T-lymphocytes after 2 and 9 Gy, z-score analyses were performed. Results: A cohort of 12 hypersensitive patients was evaluated; 8 showed enhanced early toxicity, 3 showed enhanced late toxicity, and 1 showed both. The cohort displayed less radiation-induced apoptosis (21.8 s) than average age-matched donors. A cohort of 9 ataxia telangiectasia homozygotes displayed even less apoptosis (23.6 s). Conclusion: The leukocyte apoptosis assay appears to be a useful predictor of individuals likely to display increased toxicity to radiation therapy; however, validation of this requires a prospective study. © 1999 Elsevier Science Inc. [less ▲]

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See detailFlora and vegetation of Turtell V, Turtell Islands group (Queensland, Australia).
De Sloover, Jacques; Dufrêne, Marc ULg

in Bulletin du Jardin Botanique National de Belgique = Bulletin van de Nationale Plantentuin van België (1999), 67

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See detailActualisation de la prise en charge de l'hypertension artérielle
Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (1999), (54), 683-687

The International Society of Hypertension and the World Heath Organisation have recently published new guidelines for the management of hypertension. Epidemiologic studies have shown that management of ... [more ▼]

The International Society of Hypertension and the World Heath Organisation have recently published new guidelines for the management of hypertension. Epidemiologic studies have shown that management of hypertension still remains insatisfactory. The aim of the treatment is to reduce the cardiovascular morbidity and mortality by normalizing blood pressure. The target blood pressure under treatment must be less than 140/90 mmHg, and even lower for diabetic or chronic renal failure patients. In parallel, all cardio-vascular risk factors have to be corrected to decrease the global cardio-vascular risk. [less ▲]

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See detail«Verità e metodo nella scrittura di Leonardo Sciascia»
Moreno, Paola ULg

in Esperienze letterarie (1999)

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See detailImpact de l'ammoniac sur le système respiratoire du porc. Etude de la relation dose-réponse
Gustin, Pascal ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(6, Pt 2), 371-375

The effects of ammonia on the respiratory system and somatic growth have been measured in piglets taking into account the interactions with endotoxins from gram-bacteria and dust. Dose-response curves ... [more ▼]

The effects of ammonia on the respiratory system and somatic growth have been measured in piglets taking into account the interactions with endotoxins from gram-bacteria and dust. Dose-response curves were constructed for the inflammatory reactions induced in nasal cavities, the smooth muscle hyperresponsiveness in the trachea, the permeability of alveolo-capillary barrier, the pulmonary vasomotricity, the cough reflex and the systemic effects. An acceptable level of pollution was proposed. [less ▲]

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See detailReport of the Sixth International Workshop on Human Chromosome 11 Mapping 1998. Nice, France, May 2-5, 1998.
Gaudray, P.; Carle, G. F.; Gerhard, D. S. et al

in CytoGenetics & Cell Genetics (1999), 86(3-4), 167-86

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See detailThree-dimensional Kinetic Energy Distributions of Ions using a Retarding Potential Analyser. Analysis of Discimination Effects by Ion Trajectory Simulations.
Hoxha, A.; Leyh, Bernard ULg; Locht, Robert ULg

in Rapid Communications in Mass Spectrometry : RCM (1999), 13

This paper presents a method of extracting three-dimensional kinetic energy distributions from retarding potential curves, suitable for analysing low kinetic energy releases. Trajectory calculations were ... [more ▼]

This paper presents a method of extracting three-dimensional kinetic energy distributions from retarding potential curves, suitable for analysing low kinetic energy releases. Trajectory calculations were performed which led to an analytical function correcting for the influence of discrimination against ions with velocity components perpendicular to the retarding field direction. The analysis of the thermal kinetic energy distribution of molecular vinyl bromide ions confirms the computed correcting factor. [less ▲]

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See detailLes convergences culturelles dans les sociétés pluriethniques de K. Fall et al.
Martiniello, Marco ULg

in Recherches Sociologiques (1999), 2

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See detailMorphométrie du pied du cheval et maladie naviculaire
Collin, B.; Gabriel, Annick ULg

in Bulletin de l'Académie Vétérinaire de France (1999), 72

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See detailUn réformateur lotharingien de choc : l'abbé Poppon de Stavelot (978-1048)
George, Philippe ULg

in Revue Mabillon (1999)

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See detailAnalysis of Scintigraphical Lung Images before and after Treatment of Horses Suffering from Chronic Pulmonary Disease
Votion, Dominique ULg; Ghafir, Y.; Vandenput, Sandrina ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 144(9), 232-236

Scintigraphical analysis of the ratio of inhalation (I) to perfusion (Q) was designed to determine whether chronic pulmonary disease in horses induced mismatches in I/Q and to assess whether medical ... [more ▼]

Scintigraphical analysis of the ratio of inhalation (I) to perfusion (Q) was designed to determine whether chronic pulmonary disease in horses induced mismatches in I/Q and to assess whether medical treatment would restore an I/Q distribution pattern identical to that of control horses. In addition, the results of the I/Q analysis were correlated with the alveolar-arterial PO2 difference (AaDO2). The I/Q matching found in a group of control horses was compared with the I/Q analysis of a group of diseased horses before and after their clinical signs had been treated. The analysis indicated that there was mismatching between I and Q before they were treated. The treatment improved the diseased horses, pulmonary function but there was still heterogeneity in the I/Q distribution after they had been treated. The I/Q analysis parameters were well correlated with the ratio of ventilation (VA) and pulmonary blood flow (Q), evaluated by the determination of AaDO2 [less ▲]

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See detailNote sous Anvers, 23 juin 1999
Orban, Lionel ULg

in Actualités du Droit (1999)

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See detailIndices of oxidative stress in blood and pulmonary epithelium lining fluid in horses suffering from recurrent airway obstruction
Art, Tatiana ULg; Kirschvink, Nathalie; Smith, Nicola et al

in Equine Veterinary Journal (1999), 31(5), 397-401

To test the hypothesis that reactive oxygen species could be associated to the lower airway disorders occurring in horses suffering from recurrent airway obstruction (RAO), indices of oxidative stress ... [more ▼]

To test the hypothesis that reactive oxygen species could be associated to the lower airway disorders occurring in horses suffering from recurrent airway obstruction (RAO), indices of oxidative stress were studied in blood and pulmonary epithelium lining fluid in 5 RAO horses either in clinical remission or 24 h after the onset of a crisis of bronchospasm and in 5 healthy horses. Venous blood and bronchoalveolar lavage fluid (BALF) samples were collected and analysed for reduced glutathione (GSH), oxidised glutathione (GSSG), total glutathione (TGSH), glutathione redox ratio (GRR) in blood haemolysate and pulmonary epithelium lining fluid (PELF). The haemolysate concentrations of GSH, GSSG, TGSH and GRR were similar in the 3 groups. The PELF glutathione status was significantly different in the RAO horses in acute crisis compared to healthy horses, indicating the occurrence of an oxidative stress. When RAO horses were in crisis their GSH and TGSH remained unchanged but their GSSG and GRR were significantly increased compared to the remission. These results support the hypothesis that oxidative stress is associated with lower airway disorders occurring in horses suffering from RAO [less ▲]

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See detailAnalysis of fluorescence lifetime of protochlorophyllide and chlorophyllide in isolated etioplast membranes measured from multifrequency cross-correlation phase fluorometry
Mysliwa-Kurdziel, B.; Franck, Fabrice ULg; Strzalka, K.

in Photochemistry & Photobiology (1999), 70(4), 616-623

The fluorescence decays of protochlorophyllide (Pchlide) and of chlorophyllide (Chlide) in wheat etioplast membranes were analyzed using a multiexponential fluorescence decay model. Using different ... [more ▼]

The fluorescence decays of protochlorophyllide (Pchlide) and of chlorophyllide (Chlide) in wheat etioplast membranes were analyzed using a multiexponential fluorescence decay model. Using different excitation wavelengths from 430 to 470 nm, we found that a triple-exponential model at 14 degrees C and a double-exponential model at -170 degrees C were adequate to describe the Pchlide fluorescence decay. We discuss the origin of the three fluorescence lifetime components at 14 degrees C on the basis of the dependence of their fractional intensities on the excitation wavelength and by correlating the fractional intensities with integrated fluorescence intensities of different Pchlide forms in steady-state fluorescence spectra, The fluorescence decay of the main Pchlide form, photoactive Pchlide-F657, is shown to have a complex character with a fast component of 0.25 ns and a slower component of about 2 ns. Two lifetime components of 2 ns and 5.5-6.0 ns are ascribed to the second photoactive form, Pchlide-F645, and to nonphotoactive Pchlide forms, respectively. In etioplast membranes preilluminated by a short saturating fight pulse, we found a single 5.0 ns component for Chlide-F688 (the Chlide-NADPH :protochlorophyllide oxidoreductase [POR]-NADP(+) complex) and an additional 1.6 ns component when the formation of Chlide-F696 (the Chlide-POR-NADPH complex) was promoted by exogenous NADPH, From the fluorescence lifetime results we evaluated the quantum yield of the primary photoreaction by Chlide-F696 as being 70%. [less ▲]

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See detailThe first successful crystallization of a prokaryotic extremely thermophilic outer surface layer glycoprotein
Evrard, Christine ULg; Declercq, Jean-Paul; Debaerdemaeker, Tony et al

in Zeitschrift für Kristallographie (1999), 214

Methanothermus fervidus belongs to the group of hyperthermophilic Archaea. The Archaea comprise organisms that live under environmental extremes. like high temperature, low pH value or high salt ... [more ▼]

Methanothermus fervidus belongs to the group of hyperthermophilic Archaea. The Archaea comprise organisms that live under environmental extremes. like high temperature, low pH value or high salt concentration. The outer surface of the pseudomurein sacculi of the cells of Methanothermus fervidus is covered by glycoprotein subunits (S-Iayer) directly exposed to the extreme environment. The elucidation of the crystal structure of this surface glycoprotein may provide important information on the survival strategies of these unusual micro-organisms. Before our investigations neither three-dimensional crystals have been obtained nor X-ray analyses were performed. Only electron microscopic and computer image enhancement techniques have been applied to obtain structural information from crystalline two-dimensional S-layer sheets. We describe here the successful crystallization of this surface glycoprotein under microgravity conditions. [less ▲]

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See detailLe Mélèze
Jourez, Benoît ULg; Pauwels, Dominique; Jacques, Dominique et al

in Courrier du Bois (1999), 125

De la famille des pinacées, le Mélèze, ou plutôt les Mélèzes, car ils sont trois chez nous à se partager ce titre générique, produisent un bois à la durabilité reconnue et à l'esthétique appréciée. Leurs ... [more ▼]

De la famille des pinacées, le Mélèze, ou plutôt les Mélèzes, car ils sont trois chez nous à se partager ce titre générique, produisent un bois à la durabilité reconnue et à l'esthétique appréciée. Leurs qualités technologiques sont susceptibles de satisfaire les exigences des utilisateurs dans des domaines très variés, tant en usage intérieur qu'extérieur. Cette essence n'a cependant pas la popularité qu'elle mérite auprès des sylviculteurs, malgré un potentiel de croissance élevé. [less ▲]

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See detailRacisme et sentiment d'appartenance ethnique chez les 10-12 ans en milieu scolaire
Pirotte, Gautier ULg

in Nouvelles Pratiques Sociales (1999), vol12(1/2), 217-232

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See detailNe VI spectrum
Kramida, A. E.; Bastin, Thierry ULg; Biémont, Emile ULg et al

in Journal of the Optical Society of America. B, Optical Physics (1999), 16

On the basis of all the available observations of the Ne VI spectrum, particularly the new beam-foil spectra obtained at Liège University, approximately 50 new line assignments are proposed and ... [more ▼]

On the basis of all the available observations of the Ne VI spectrum, particularly the new beam-foil spectra obtained at Liège University, approximately 50 new line assignments are proposed and approximately 30 new energy levels are determined in Ne VI. All known energy levels have been improved, and a new value is proposed for the ionization potential. The new line identifications are supported by Hartree–Fock calculations combined with parametric fitting of energies and transition rates. [less ▲]

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See detailDiscours de réception à l’Académie royale de langue et de littérature françaises (séance publique du 5 juin 1999)
Droixhe, Daniel ULg

in Bulletin de l'Académie Royale de Langue et de Littérature Françaises (1999), LXXVII(1-2), 67-78

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See detailEffect of hyonate administration on pulmonary function and indices of oxidative stress in COPD horses in clinical remission at rest and after exercise
Art, Tatiana ULg; Kirschvink, Nathalie; Le Sueur, C. et al

in Journal of Equine Veterinary Science (1999), 19

Pulmonary function tests, bronchoalveolar lavages, and venous blood samplings were performed 24 hours before and one hour after a standardized strenous treadmill exercise test on six horses suffering from ... [more ▼]

Pulmonary function tests, bronchoalveolar lavages, and venous blood samplings were performed 24 hours before and one hour after a standardized strenous treadmill exercise test on six horses suffering from chronic obstructive pulmonary disease (COPD) in clinical remission. Pulmonary function test consisted of the measurement of the mechanics of breathing and arterial blood gases analyses. During the exercise tests, heart and respiratory rates, and venous plasma lactate, pH and packed cell volume were controlled. Venous blood was analyzed for some markers of the oxidative stress, i.e., hemolysate glutathione redox ratio and oxidized glutathione, and plasma uric acid. BAL was analyzed for cell cytology as well as for markers of oxidative stress, i.e., glutathione redox ratio, and isoprostanes concentration. The procedure was repeated twice, i.e., before and after two IV injections of 40mg hyaluronate administered at seven day intervals. The results were compared to assess a possible influence of the treatment on the pulmonary and systemic inflammatory and/or oxidative status of these horses. Hyaluronate did not influence the pulmonary function test or the physiological parameters recorded during exercise. There was a decrease in the percentage of BAL neutrophils after the treatment, suggesting a possible anti-inflammatory effect at the pulmonary level. As well, the concentration of isoprostanes in the pulmonary epithelium lining fluid was significantly lower after the treatment, which might be either a consequence of the decrease of the BAL neutrophils, or related to a possible antioxidant effect of the treatment. Lastly, the exercise-induced increase in plasma uric acid was significantly less marked after the treatment, which could be due to a systemic antioxidant effect of the hyaluronate. In conclusion, this preliminary study brings some evidence that hyaluronate could have some antiinflammatory and antioxidant effects at the pulmonary—and systemic—level in COPD horses in remission. However, it does not help to understand the possible mechanisms underlying these observations. Further studies are now necessary to better understand its action, to assess the effect of the product on the pulmonary function of horses suffering from other respiratory problems as well as to assess the efficiency of other ways of administration, i.e., nebulization [less ▲]

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See detailNon-invasive assessment of arterial haemoglobin oxygen saturation in cattle by pulse oximetry
Coghe, Joost; Uystepruyst, Christophe; Bureau, Fabrice ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 145

The aim of this study was to evaluate the practicality and accuracy of different attachment sites for the optodes of a pulse oximeter (measuring arterial haemoglobin oxygen saturation) in healthy cattle ... [more ▼]

The aim of this study was to evaluate the practicality and accuracy of different attachment sites for the optodes of a pulse oximeter (measuring arterial haemoglobin oxygen saturation) in healthy cattle, and to assess the accuracy of pulse oximetry in diseased cattle with low haemoglobin oxygen saturation values caused by respiratory disease. The tait the nasal septum and the genital mucosa of females provided a continuous, stable and intense signal. The smallest bias, and no significant difference between measurements of arterial haemoglobin (mSp02) with the pulse oximeter and measurements of arterial haemoglobin oxygen saturation (SaOZ) with a blood gas analyser was obtained when the probe was attached to the tail. This site was used to evaluate the accuracy of pulse oximetry in animais with respiratory disease. There was a small bias between the measurements of Sa02 and mSp02, with a tendency for pulse oximetry to underestimate higher values and to overestimate lower values. The precision of pulse oximetry decreased substantially with the values for Sa02 <80 per cent, which lies outside the clinically relevant range [less ▲]

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See detailPatterns of seasonal and interannual changes of surface chlorophyll concentration in the Black Sea revealed from the remote sensed data.
Nezlin, Nikolay Pavlovich; Kostianoy, Andrey G.; Grégoire, Marilaure ULg

in Remote Sensing of Environment (1999), 69

Several years of CZCS-measured surface pigment’s ecosystems (e.g., Shushkina et al., 1995; Vinogradov et concentrations in the Black Sea are analyzed to appraise al., 1995; 1996a,b; Nihoul et al., 1998 ... [more ▼]

Several years of CZCS-measured surface pigment’s ecosystems (e.g., Shushkina et al., 1995; Vinogradov et concentrations in the Black Sea are analyzed to appraise al., 1995; 1996a,b; Nihoul et al., 1998). The analysis of the seasonal and year-to-year fluctuations of phytoplank- the images of the ocean color collected with the help of ton biomass and understand the causes of these fluctua- remote sensing seems to be one of the most productive tions in terms of the Black Sea’s general dynamics. The methods of estimation the general patterns of temporal pattern of seasonal variations is typical for subtropical and spatial variations of plant pigment concentration in rather than temperate regions. The range of the absolute surface layer of sea water. value of plant pigment surface concentration measured The chlorophyll concentrations measured by remote by remote sensing does not differ greatly from the values sensing methods are known to be the subject of serious measured by direct methods. The pattern of year-to-year discrepancies as compared with in situ measurements variations seems to correlate with cyclic oscillations of (e.g., Chavez, 1995; Martin and Perry, 1994; Mitchell, winter air temperature. In western shallow regions it is 1992; Nihoul et al., 1998). However, these observations also correlated with the Danube discharge intensity. are rather regular and numerous; thus they are worth at- More intensive winter–spring blooms and a slightly lower tention for the analysis of the variations of the Black level of pigment concentration during warm season are Sea’s ecosystem. typical for years of with a mild winter. The causes of The Coastal Zone Color Scanner (CZCS) was develthese regularities seem to be the peculiarities of hydrolog- oped by NASA. It was launched on the Nimbus-7 satelical and meteorological regimes of the Black Sea. The in- lite in October 1978. During its 7.5 year lifetime (Octotensity of winter–spring bloom of phytoplankton appears ber 1978–June 1986), the CZCS acquired nearly 68,000 to depend on hydrological mechanism (i.e., the intensity images, each covering up to 2 million square kilometers of water mixing during winter period due to thermic con- of ocean surface. The Nimbus Project Office in collabovection and wind mixing) rather than the illumination in- ration with the National Aeronautics and Space Administensity. [less ▲]

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See detailIonization potentials of atoms and ions from lithium to tin (Z = 50)
Biémont, Emile ULg; Frémat, Y.; Quinet, Pascal ULg

in At. Data Nucl. Data Tables (1999), 71

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See detailA locus for febrile seizures (FEB3) maps to chromosome 2q23-24.
Peiffer, A.; Thompson, J.; Charlier, Carole ULg et al

in Annals of Neurology (1999), 46(4), 671-8

Febrile seizures are the most common form of childhood seizures, occurring in 2% to 5% of North American children. We report a large Utah family with 21 members affected by febrile seizures inherited as ... [more ▼]

Febrile seizures are the most common form of childhood seizures, occurring in 2% to 5% of North American children. We report a large Utah family with 21 members affected by febrile seizures inherited as an autosomal dominant trait. All had generalized tonic-clonic seizures with onset associated with fever, consistent with the consensus febrile seizure phenotype, and none had febrile seizures beyond 6 years of age. Eighteen affected individuals had recurrent febrile seizures. Eight individuals developed afebrile seizures between ages 5 and 13 years. Afebrile seizures consisted of generalized tonic-clonic, generalized tonic, generalized atonic, simple partial, and partial complex seizure types and were associated with abnormal electroencephalographic findings in 5 individuals, all of whom were intellectually normal. We undertook linkage analysis in this family, defining the disease phenotype as febrile seizures alone. Linkage analysis in epilepsy candidate gene/loci regions failed to show evidence for linkage to febrile seizures. However, a genomewide scan and subsequent fine mapping revealed significant evidence for a new febrile seizure locus (FEB3) on chromosome 2q23-24 with linkage to the marker D2S2330 (LOD score 8.08 at theta = 0.001). Haplotype analysis defined a critical 10-cM region between markers D2S141 and D2S2345 that contains the FEB3 locus. [less ▲]

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See detailLymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
Coumans, Bernard ULg; Thellin, Olivier ULg; Zorzi, Willy ULg et al

in Journal of Immunological Methods (1999), 224(1-2), 185-196

To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts ... [more ▼]

To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailLimited clinical utility of a self-evaluating risk assessment scale for postmenopausal osteoporosis: lack of predictive value of lifestyle-related factors
Goemaere, S; Zegels, Brigitte ULg; Toye, K et al

in Calcified Tissue International (1999), 65(5), 354-358

The aim of this study was to assess the efficiency of a self-administered questionnaire to identify subjects with postmenopausal osteoporosis in the setting of first line medical care. A sample of 300 ... [more ▼]

The aim of this study was to assess the efficiency of a self-administered questionnaire to identify subjects with postmenopausal osteoporosis in the setting of first line medical care. A sample of 300 postmenopausal women completed the questionnaire based on 18 items. Bone mineral density at the lumbar spine (BMD-L), total hip (BMD-H), and femoral neck (BMD-N) was used as objective criterion for evaluation. The mean risk score was 8.2 +/- 3.21. BMD was correlated with total risk score: r = -0.32 for BMD-L, -0.36 for BMD-N, and -0.43 for BMD-H. Cutoff points for the risk score (equal likelihood points) according to a T-score threshold of -2.5 were 8.6 for BMD-L and BMD-N and 9.3 for BMD-H; specificity and sensitivity was 62% and 62%, respectively, for BMD-L, 65% and 62% for BMD-N, and 75% and 63% for BMD-H. Stepwise multiple regression analysis of the questionnaire items in relation to BMD showed higher correlation coefficients for models including individual items rather than the overall risk score. Items concerning low weight, older age, and wrist fracture after 50 years of age were always selected as significant determinants of BMD (R = 0.43-0.55). Hormonal replacement therapy was also an important determinant. Lifestyle-related items did not contribute significantly. In conclusion, the diagnostic performance of the 18-item self-administered questionnaire was poorer than a shortened questionnaire omitting lifestyle factors. The clinical utility of a questionnaire should ultimately be evaluated in the specific optic of a chosen global strategy for prevention of osteoporotic fractures. [less ▲]

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See detailUne néoplasie colique de cause endocrinienne…
Beckers, Albert ULg

in Médecine et Thérapeutique (1999), 5(2), 153-159

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