References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailArterial elastance and heart-arterial coupling in aortic regurgitation are determined by aortic leak severity
Segers, P.; Morimont, Philippe ULg; Kolh, Philippe ULg et al

in American Heart Journal (2002), 144(4), 568-576

Background In aortic valve regurgitation (AR), aortic leak severity modulates left ventricle (LV) arterial system interaction. The aim of this study was to assess (1) how arterial elastance (E-a ... [more ▼]

Background In aortic valve regurgitation (AR), aortic leak severity modulates left ventricle (LV) arterial system interaction. The aim of this study was to assess (1) how arterial elastance (E-a), calculated as the ratio of LV end-systolic pressure and stroke volume, relates to arterial properties and leak severity and (2) the validity of E-a/E-max (with E-max the slope of the-end-systolic pressure-volume relation) as a heart-arterial coupling parameter in AR. Methods and Results Our work is based on human data obtained from a study on vascular adaptation in chronic AR. These data allowed us to assess the parameters of a computer model of heart-arterial interaction. In particular, total peripheral resistance (R) and aortic leak severity-expressed as leak resistance (R-L,R-ao)-were quantified for different patient subgroups (group I/IIa/IIb: E-max = 2.15/0.62/0.47 mm Hg/mL; E-a = 1.24/0.66/0.90 mm Hg/mL; R = 1.9/0.6/0.85 mm Hg-s/mL, R-L,R-ao = 0.35/0.05/0.20 mm Hg-s/mL). A parameter study demonstrated that R-L,R-ao was the main determinant of E-a. With all other parameters constant, valve repair would increase E-a to 2.81, 1.08, and 1.54 mm Hg/mL in groups I,IIa, and IIb, respectively. For a given E-a/E-max, LV pump efficiency (estimated as the ratio of stroke work and LV systolic pressure-volume area) was lower than the theoretical predicted value, except for the simulations with intact aortic valve. Conclusions In AR(a) E-a is determined by aortic leak severity rather than by arterial system properties. Using E-a/E-max as a coupling parameter in general or as a mechanico-energetic regulatory parameter in particular is questionable. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
Peer Reviewed
See detailEpithelial supporting cells can differentiate into outer hair cells and Deiters' cells in the cultured organ of Corti
Malgrange, Brigitte ULg; Thiry, Marc ULg; Van De Water, Thomas R. et al

in Cellular and Molecular Life Sciences : CMLS (2002), 59(10), 1744-1757

The organ of Corti is a complex structure containing a single row of inner hair cells (IHCs) and three rows of outer hair cells (OHCs), supported respectively by one row of inner phalangeal cells and ... [more ▼]

The organ of Corti is a complex structure containing a single row of inner hair cells (IHCs) and three rows of outer hair cells (OHCs), supported respectively by one row of inner phalangeal cells and three rows of Deiters' cells. When fetal rat organ of Corti explants are cultured, supernumerary OHCs and supernumerary Deiters' cells are produced, without any additional cell proliferation. Analysis of semi- and ultrathin sections revealed that supernumerary OHCs are produced at the distal edge of the organ of Corti. Quantitative analysis of cell types present in the organ of Corti demonstrates that when the number of OHCs increases: (i) the total number of cells remains constant; (ii) the number of Deiters' cells increases; (iii) the number of tectal cells decreases and of Hensen's cells decreases. Using specific HC markers, i.e. jagged2 (Jag2) and Math1, we showed that in addition to existing OHCs, supernumerary OHCs, tectal cells and Hensen's cells expressed these markers in embryonic day 19 organ of Corti explants after 5 days in vitro. The results of this study suggest that Hensen's cells retain the capacity to differentiate into either tectal cells, which differentiate into OHCs, or into undertectal cells which differentiate into Deiters' cells. [less ▲]

Detailed reference viewed: 19 (4 ULg)
Full Text
Peer Reviewed
See detailCyclosporin-A differentially affects apoptosis during in vivo rat thymocyte maturation
Damoiseaux, JGMC; Defresne, Marie-Paule ULg; Reutelingsperger, C. P. M. et al

in Scandinavian Journal of Immunology (2002), 56(4), 353-360

Maturation arrest and interference with selection are two well-documented effects of cyclosporin-A (CsA) on the thymus. We recently hypothesized that these effects are related and owing to the reduced T ... [more ▼]

Maturation arrest and interference with selection are two well-documented effects of cyclosporin-A (CsA) on the thymus. We recently hypothesized that these effects are related and owing to the reduced T-cell receptor (TCR)-CD3 complex-mediated signal transduction in thymocytes upon CsA treatment. In this hypothesis, the maturation arrest is the result of the additional depletion of thymocytes that normally survive by positive selection, whereas the impaired self-tolerance induction is caused by an increased survival of thymocytes that normally undergo negative selection. In this view, it is anticipated that CsA differentially affects thymocyte apoptosis during in vivo thymocyte maturation. Indeed, we report in this study a strong increase in apoptotic cells in the thymic cortex on in situ analysis. Simultaneously, the number of apoptotic cells had decreased at the cortico-medullary zone which is held to be the site for negative selection. Rapamycin (Rapa) also interferes with thymocyted maturation by inhibiting cytokine-driven proliferation. Hence, Rapa preferentially affects the early maturational stages of thymoctye development and is considered not to alter thymocyte selection and subsequent apoptotic events. Indeed, the number of apoptotic events appears not to be altered. However, possibly owing to the decrease in cortical macrophages, the apoptotic cells revealed an atypical enumeration around blood vessels. Taken together, our results favour the hypothesis that the dominant effect of CsA on the thymus is the reduction of the TCR-CD3 complex-mediated signal transduction in thymocytes upon interaction with stromal cells. Furthermore, the preferential localization of apoptotic cells next to blood vessels upon Rapa administration may indicate that endothelial cells are a back-up system for the removal of apoptotic cells. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailSecreted metalloprotease gene family of Microsporum canis
Brouta, Frédéric; Descamps, Frédéric; Monod, Michel et al

in Infection and Immunity (2002), 70(10), 5676-5683

Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently ... [more ▼]

Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲]

Detailed reference viewed: 38 (2 ULg)
Full Text
Peer Reviewed
See detailEen Nederlandstalige Shelley op de planken. De reconstructie van een vergeten schandaal
Steyaert, Kris ULg

in Neerlandica Extra Muros (2002), 40(3), 13-25

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailTraitement hormonal de la menopause: traitement hormonal substitutif classique ou tibolone?
Reginster, Jean-Yves ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2002), 31(6), 541-9

OBJECTIVE: Our purpose was to review with critical analysis, data from randomized controlled clinical trials comparing the effects on bone of conventional hormone replacement therapy (HRT) and tibolone ... [more ▼]

OBJECTIVE: Our purpose was to review with critical analysis, data from randomized controlled clinical trials comparing the effects on bone of conventional hormone replacement therapy (HRT) and tibolone. Their respective effects on bone were also reviewed and summarized. Materials and methods. Medline via PubMed was searched using a combination of the following key words "tibolone, estrogens and bone" to identify all randomized controlled trials tibolone versus HRT (1960-2001). RESULTS: Six randomized controlled trials that have been carried out to compare effects of tibolone and conventional HRT on prevention of postmenopausal bone loss were identified. Only one of these trials has been performed with a correct methodology (double-blind and with an adequate duration). In this trial over 2 years, the highest significant increase from baseline in bone mineral density (BMD) at all sites was observed with HRT; in addition, HRT showed a significantly greater increase in BMD at lumbar spine than tibolone. Randomized placebo-controlled trials have demonstrated that tibolone produces positive effects on BMD. Nevertheless, no clinical convincing data are available to support its efficacy in the prevention of osteoporotic fractures. The positive impact on BMD of conventional HRT to prevent postmenopausal bone loss and to treat established osteoporosis has been shown by many randomized controlled trials. Regarding fracture risk prevention, some clinical and epidemiological data suggest that HRT initiated in early or late postmenopause may prevent fractures if it is administered at standard doses and continued for a long time. CONCLUSION: HRT is always the reference treatment of postmenopausal symptoms related to estrogen deficiency. [less ▲]

Detailed reference viewed: 136 (0 ULg)
Full Text
Peer Reviewed
See detailThe lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways.
Dejardin, Emmanuel ULg; Droin, Nathalie; Delhase, Mireille et al

in Immunity (2002), 17

The lymphotoxin-beta receptor (LTbetaR) plays critical roles in inflammation and lymphoid organogenesis through activation of NF-kappaB. In addition to activation of the classical NF-kappaB, ligation of ... [more ▼]

The lymphotoxin-beta receptor (LTbetaR) plays critical roles in inflammation and lymphoid organogenesis through activation of NF-kappaB. In addition to activation of the classical NF-kappaB, ligation of this receptor induces the processing of the cytosolic NF-kappaB2/p100 precursor to yield the mature p52 subunit, followed by translocation of p52 to the nucleus. This activation of NF-kappaB2 requires NIK and IKKalpha, while NEMO/IKKgamma is dispensable for p100 processing. IKKbeta-dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta, and MIP-2 in response to LTbetaR ligation. In contrast, IKKalpha controls the induction by LTbetaR ligation of chemokines and cytokines involved in lymphoid organogenesis, including SLC, BLC, ELC, SDF1, and BAFF. [less ▲]

Detailed reference viewed: 25 (4 ULg)
Full Text
Peer Reviewed
See detailTransition probabilities in GdIII
Biémont, Emile ULg; Kohnen, G.; Quinet, Pascal ULg

in Astronomy and Astrophysics (2002), 393(2), 717-720

Theoretical lifetimes, calculated with inclusion of core-polarization effects, have been determined for five 4f(7) 6p levels of doubly ionized gadolinium. They agree quite well with recent experimental ... [more ▼]

Theoretical lifetimes, calculated with inclusion of core-polarization effects, have been determined for five 4f(7) 6p levels of doubly ionized gadolinium. They agree quite well with recent experimental values measured by time-resolved laser-induced fluorescence spectroscopy. From this agreement, the accuracy of a first set of Gd III transition probabilities, calculated for 4f(7) 5d- 4f(7) 6p and 4f(7) 6s- 4f(7) 6p transitions of astrophysical interest, has been assessed. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailSputum eosinophil count in a large population of patients with mild to moderate steroid-naive asthma: distribution and relationship with methacholine bronchial hyperresponsiveness
Louis, Renaud ULg; Sele, Jocelyne ULg; Henket, Monique ULg et al

in Allergy (2002), 57(10), 907-912

BACKGROUND: Although airway eosinophilia is seen as a cardinal feature of asthma, data eosinophilia are still lacking on the proportion of the asthma group exhibiting raised airway eosinophilia. This ... [more ▼]

BACKGROUND: Although airway eosinophilia is seen as a cardinal feature of asthma, data eosinophilia are still lacking on the proportion of the asthma group exhibiting raised airway eosinophilia. This study aimed to assess the distribution of sputum eosinophil count and its relationship with methacholine bronchial hyperresponsiveness in mild to moderate steroid-naive asthmatic people. METHODS: Sputum was induced by inhalation of hypertonic saline (NaCl 4.5%) in 118 mild to moderate steroid-naive asthmatic people consecutively recruited from our outpatient clinic, and in 44 healthy people. The asthma group was selected on the basis of an forced expiratory volume in 1 s (FEV(1)) of > or = 70% predicted, and a provocative methacholine concentration causing a fall of 20% in FEV(1) (PC20 methacholine; PC(20)M) < or = 16 mg/ml. RESULTS: In the asthma group, the median (range) of the percentage and the absolute values of sputum eosinophils were 4.8% (0-75) and 38 10(3)/g (0-14,191), respectively, vs 0% (0-2.3) (P < 0.001) and 0 10(3)/g (0-53) (P < 0.001) in healthy participants. Based on the 95% percentile for normal values calculated from our healthy group, 69% of the asthma group had significantly raised sputum eosinophil count (that is > 2%). In the asthma group, multiple regression analysis followed by a stepwise procedure revealed that sputum eosinophil count was significantly and inversely associated with PC(20)M accounting for 16% of its total variance (P < 0.001) while neutrophil counts positively related to PC(20)M accounting for 4% of total variance (P < 0.05). By contrast, no significant relationship was found between either eosinophil or neutrophil counts and the slope of forced vital capacity (FVC) vs FEV(1) from the methacholine challenge. CONCLUSIONS: We conclude that two-thirds of people in the mild to moderate asthma group had increased sputum eosinophilia, which plays a limited role in determining the degree of methacholine airway hyperresponsiveness. [less ▲]

Detailed reference viewed: 77 (7 ULg)
Full Text
Peer Reviewed
See detailLa notion de 'parliamentary sovereignty' en droit constitutionnel anglais : fondements, paradoxes et problèmes contemporains
Behrendt, Christian ULg

in Revue de Droit International et de Droit Comparé (2002)

The article, written in French, gives an outline on the concept of parliamentary sovereignty in UK constitutional law.

Detailed reference viewed: 290 (32 ULg)
Full Text
Peer Reviewed
See detailAntiplasmodial activity of alkaloids from various Strychnos species
Frederich, Michel ULg; Jacquier, M. J.; Thepenier, P. et al

in Journal of Natural Products (2002), 65(10), 1381-1386

The in vitro antiplasmodial activities of 69 alkaloids from various Strychnos species were evaluated against chloroquine-resistant and chloroquine-sensitive lines of Plasmodium falciparum. The compounds ... [more ▼]

The in vitro antiplasmodial activities of 69 alkaloids from various Strychnos species were evaluated against chloroquine-resistant and chloroquine-sensitive lines of Plasmodium falciparum. The compounds, comprising mainly indolomonoterpenoid alkaloids, exhibited a wide range of biological potencies in the antiplasmodial assays. The most active alkaloids were also tested for cytotoxicity against HCT-116 colon cancer cells to determine their antiplasmodial selectivity. As a result of these studies, structure-activity relationships for these alkaloids have begun to emerge. Alkaloids presenting four types of bisindole skeleton exhibited potent and selective activities against Plasmodium. They were sungucine-type (IC(50) values ranging from 80 nM to 10 microM), longicaudatine-type (IC(50) values ranging from 0.5 to 10 microM), matopensine-type (IC(50) values ranging from 150 nM to 10 microM), and usambarine-type alkaloids. Within the last structural type, isostrychnopentamine (49) and ochrolifuanine A (46) were found to be active against chloroquine-sensitive and -resistant strains (IC(50) values of 100-150 and 100-500 nM, respectively), and dihydrousambarensine (51) exhibited a 30-fold higher activity against the chloroquine-resistant strain (IC(50) = 32 nM) than against the chloroquine-sensitive one. [less ▲]

Detailed reference viewed: 55 (7 ULg)
Full Text
Peer Reviewed
See detailExploring brain connectivity: a new frontier in systems neuroscience
Ramnani, Narander; Lee, Lucy; Phillips, Christophe ULg et al

in Trends in Neurosciences (2002), 25(10), 496-497

Detailed reference viewed: 28 (1 ULg)
Full Text
Peer Reviewed
See detailA family of MMA approximations for structural optimization
Bruyneel, Michaël ULg; Duysinx, Pierre ULg; Fleury, Claude ULg

in Structural and Multidisciplinary Optimization (2002), 24(4), 263-276

This paper proposes a new first-order approximation scheme used for solving structural optimization problems. It is based on approximations of the MMA family (MMA and GCMMA), but it utilizes the gradients ... [more ▼]

This paper proposes a new first-order approximation scheme used for solving structural optimization problems. It is based on approximations of the MMA family (MMA and GCMMA), but it utilizes the gradients and/or the function values at two successive design points to improve the quality of the approximation. In addition, this scheme can consider simultaneously monotonous and nonmonotonous structural behaviour. According to the characteristics of the treated problem, one of the approximations or a mix of them is automatically selected. Based on this approach, the accuracy of the approximated subproblems is improved and the solution process can be sped up. Numerical results compare the effectiveness of the method with previously derived approximations of the MMA family for shape optimization of trusses and for composite design problems. The benefit of using mixed approximations is also discussed. [less ▲]

Detailed reference viewed: 148 (24 ULg)
Full Text
Peer Reviewed
See detailContribution of whole-body (18)FDG PET imaging in the management of cervical cancer
Belhocine, T.; Thille, Alain ULg; Fridman, Viviana ULg et al

in Gynecologic Oncology (2002), 87(1), 90-97

OBJECTIVE: The objective of this study was to assess the contribution of [(18)F]fluoro-2-deoxy-D-glucose positron emission tomography ((18)FDG PET) imaging in the management of cervical cancer. METHODS ... [more ▼]

OBJECTIVE: The objective of this study was to assess the contribution of [(18)F]fluoro-2-deoxy-D-glucose positron emission tomography ((18)FDG PET) imaging in the management of cervical cancer. METHODS: Fully corrected whole-body PET was performed in 60 patients (pts) with proven cervical cancer. In pretreatment staging, 22 pts underwent PET in addition to routine protocol including International Federation of Obstetrics and Gynecology (FIGO) staging and pelvic magnetic resonance imaging (MRI). Eighteen of them had pelvic lymphadenectomy. After treatment, PET was performed in 38 pts routinely followed up by clinical and radiological examinations. Results of PET and routine protocols were compared to final diagnoses, including histological findings in 31 pts and clinical outcomes in the other cases. Median follow-up time was 12 +/- 7.3 months. RESULTS: In all but 2 patients (FIGO stage IA), both PET and MRI detected the primary tumor. In 6 pts, MRI alone noted loco-regional tumor spread but PET localized 9 unsuspected extrapelvic nodal sites (6 para-aortic, 2 mediastinal, and 1 supra-clavicular). However, PET missed 8 microscopic pelvic nodal metastases. In 18% of the patients, PET staging significantly influenced the treatment choices. In follow-up, PET accurately diagnosed a recurrent disease in 13 pts with falsely negative or equivocal conventional imaging (CI). Ten patients with a negative PET were still in complete remission after a minimal follow-up time of 12 months. Overall, the agreement of PET with final diagnosis was significantly better than that of routine protocol (P < 0.05). CONCLUSIONS: Whole-body (18)FDG PET appears useful in the management of cervical cancer, in particular for staging extrapelvic metastases or optimally detecting a recurrence. MRI is better indicated for evaluating the loco-regional status of the disease. [less ▲]

Detailed reference viewed: 42 (3 ULg)
Full Text
Peer Reviewed
See detailEffects of the social conditions of housing through testing on cocaine-induced contextual sensitisation and conditioned locomotion in C57BL/6J mice
Michel, Alexa ULg; Tirelli, Ezio ULg

in Progress in Neuro-Psychopharmacology & Biological Psychiatry (2002), 26(6), 1185-1191

The potential differential effects of isolated and collective housing through the testing phase on sensitisation to cocaine-induced locomotion, the subsequent conditioned locomotion and the context ... [more ▼]

The potential differential effects of isolated and collective housing through the testing phase on sensitisation to cocaine-induced locomotion, the subsequent conditioned locomotion and the context-dependent expression of sensitisation were examined in C57BL/6J male mice. [less ▲]

Detailed reference viewed: 21 (2 ULg)
Full Text
Peer Reviewed
See detailSecretion of alpha-amylase from Pseudoalteromonas haloplanktis TAB23: Two different pathways in different hosts
Tutino, M. L.; Parrilli, E.; Giaquinto, L. et al

in Journal of Bacteriology (2002), 184(20), 5814-5817

Secretion of cold-adapted alpha-amylase from Pseudoalteromonas haloplanktis TAB23 was studied in three Antarctic bacteria. We demonstrated that the enzyme is specifically secreted in the psychrophilic ... [more ▼]

Secretion of cold-adapted alpha-amylase from Pseudoalteromonas haloplanktis TAB23 was studied in three Antarctic bacteria. We demonstrated that the enzyme is specifically secreted in the psychrophilic hosts even in the absence of a protein domain that has been previously reported to be necessary for alpha-amylase secretion in Escherichia coli. The occurrence of two different secretion pathways in different hosts is proposed. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
See detailLa gauche a-t-elle peur de la sécurité ?
Verjans, Pierre ULg

in Politique : Revue de Débats (2002)

La genèse des partis de gauche ne les pousse pas à valoriser la défense des grands propriétaires mais la responsabilité politique et l'embourgeoisement de la classe ouvrière les pousse à défendre le ... [more ▼]

La genèse des partis de gauche ne les pousse pas à valoriser la défense des grands propriétaires mais la responsabilité politique et l'embourgeoisement de la classe ouvrière les pousse à défendre le patrimoine des petits propriétaires, d'où une tension interne difficile à gérer. [less ▲]

Detailed reference viewed: 19 (4 ULg)
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Dissection aortique de type A post-remplacement valvulaire aortique
TCHANA-SATO, Vincent ULg; RADERMECKER, Marc ULg; Pierard, Luc ULg et al

in Revue Médicale de Liège (2002), 57(10), 637-9

The risk for aortic dissection or further dilation of the ascending aorta exists after aortic valve replacement. We present a case of acute aortic dissection following replacement of the aortic valve. The ... [more ▼]

The risk for aortic dissection or further dilation of the ascending aorta exists after aortic valve replacement. We present a case of acute aortic dissection following replacement of the aortic valve. The management and treatment of dilated ascending aorta associated with aortic valve disease are reviewed and discussed. [less ▲]

Detailed reference viewed: 188 (3 ULg)
Full Text
Peer Reviewed
See detailIsolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo
Descamps, Frédéric; Brouta, Frédéric; Monod, Michel et al

in Journal of Investigative Dermatology (2002), 119(4), 830-835

Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal ... [more ▼]

Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal virulence factors are secreted keratinolytic proteases, whose molecular characterization would be an important step towards the understanding of dermatophytic infection pathogenesis. M. canis secretes a 31.5 kDa keratinolytic subtilisin-like protease as the major component in a culture medium containing cat keratin as the sole nitrogen source. Using a probe corresponding to a gene's internal fragment, which was obtained by polymerase chain reaction, the entire gene encoding this protease named SUB3 was cloned from a M. canis lambdaEMBL3 genomic library. Two closely related genes, termed SUB1 and SUB2, were also cloned from the library using as a probe the gene coding for Aspergillus fumigatus 33 kDa alkaline protease (ALP). Deduced amino acid sequence analysis revealed that SUB1, SUB2, and SUB3 are secreted proteases and show large regions of identity between themselves and with subtilisin-like proteases of other filamentous fungi. Interestingly, mRNA of SUB1, SUB2, and SUBS were detected by reverse transcriptase nested-polymerase chain reaction from hair of experimentally infected guinea pigs. These results show that SUB1, SUB2, and SUB3 encode a family of subtilisin-like proteases and strongly suggest that these proteases are produced by M. canis during the invasion of keratinized structures. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲]

Detailed reference viewed: 44 (6 ULg)
Full Text
See detailFaites-vous de la grammaire, un peu, beaucoup, passionnément, pas du tout ?
Defays, Jean-Marc ULg

in Echec à l'Echec (2002), 151

Detailed reference viewed: 30 (8 ULg)
Full Text
Peer Reviewed
See detailMatrix metalloproteinase-9 contributes to choroidal neovascularization
Lambert, Vincent ULg; Munaut, Carine ULg; Jost, M. et al

in American Journal of Pathology (2002), 161(4), 1247-1253

Age-related macular degeneration (AMD) is the primary cause of irreversible photoreceptors loss in adult patients and current therapies are limited. Increased levels of matrix metalloproteinases (MMPs ... [more ▼]

Age-related macular degeneration (AMD) is the primary cause of irreversible photoreceptors loss in adult patients and current therapies are limited. Increased levels of matrix metalloproteinases (MMPs) have been documented in neovascularization of severe ocular pathologies such as AMD and proliferative diabetic retinopathy. We report here that MMP-9 (gelatinase B) expression is induced and temporally regulated in the course of experimental choroidal neovascularization. We used transgenic mice expressing beta-galactosidase reporter gene under the dependence of MMP-9 promoter and RT-PCR analysis on choroidal neovascular structures microdissected from serial sections by laser pressure catapulting to show that MMP-9 expression is up-regulated concomitantly with the appearance of inflammatory cells in the subretinal lesion. In mice deficient in MMP-9 expression the development of choroidal neovascularization induced by laser photocoagulation still occurred, but at a reduced level. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailNeurological complications of acute and persistent Epstein-Barr virus infection in paediatric patients
Häusler, M.; Ramaekers, Vincent ULg; Doenges, M. et al

in Journal of Medical Virology (2002), 68(2), 253-263

Neurological complications of Epstein-Barr virus (EBV) have been reported almost exclusively in the course of acute primary infections. The role of EBV in paediatric neurological disease was investigated ... [more ▼]

Neurological complications of Epstein-Barr virus (EBV) have been reported almost exclusively in the course of acute primary infections. The role of EBV in paediatric neurological disease was investigated prospectively over a 2-year period, searching for acute primary, chronic, and reactivated EBV infections. Active EBV infections were diagnosed in 10/48 patients, including two with acute primary EBV infections (cranial neuritis and cerebellitis), one with chronic active infection (T/NK cell lymphoma with cranial neuritis), and seven with reactivated infections. Among these seven patients, three showed "Alice in Wonderland" syndrome, one facial nerve palsy, one progressive macrocephaly, and two prolonged encephalitic illness. The prognosis was good except for the patient with lethal T/NK cell lymphoma and the two girls with encephalitic illness. Despite steroid treatment, these girls suffered prolonged cognitive impairment and epileptic seizures. Both developed left-sided hippocampal atrophy, and one of them hippocampal sclerosis. Like primary infections, reactivated EBV infections cause neurological complications in a considerable number of paediatric patients, lead to serious long-term complications, and may contribute to the pathogenesis of hippocampal lesions. © 2002 Wiley-Liss, Inc. [less ▲]

Detailed reference viewed: 31 (6 ULg)
Full Text
Peer Reviewed
See detailThermal instability of a rotating saturated porous medium heated from below and submitted to rotation
Desaive, Thomas ULg; Hennenberg, M.; Lebon, Georgy ULg

in European Physical Journal B -- Condensed Matter (2002), 29(4), 641-647

In this work, we study the problem of onset of thermal convection in a rotating saturated porous medium heated from below. The effect of rotation is restricted to the Coriolis force, neglecting thus the ... [more ▼]

In this work, we study the problem of onset of thermal convection in a rotating saturated porous medium heated from below. The effect of rotation is restricted to the Coriolis force, neglecting thus the centrifugal effects, the porous medium is described by Brinkman's model. The linear eigenvalue problem is solved by means of a modified Galerkin method. The behavior of the critical temperature gradient is discussed in terms of various parameters of the system for both stationary and overstable convections. Finally a weakly nonlinear analysis is provided to derive amplitude equations and to study the onset of Kuppers-Lortz instability. [less ▲]

Detailed reference viewed: 28 (1 ULg)
Full Text
Peer Reviewed
See detailProduction of proinflammatory cytokines and myocardial dysfunction after arterial switch operation in neonates with transposition of the great arteries.
Hovels-Gurich, Hedwig H; Vazquez-Jimenez, Jaime F; Silvestri, Anna et al

in Journal of Thoracic and Cardiovascular Surgery (The) (2002), 124(4), 811-20

OBJECTIVE: Neonates undergoing cardiac surgery have a systemic inflammatory reaction with release of proinflammatory cytokines, which could be responsible for myocardial dysfunction as a result of ... [more ▼]

OBJECTIVE: Neonates undergoing cardiac surgery have a systemic inflammatory reaction with release of proinflammatory cytokines, which could be responsible for myocardial dysfunction as a result of myocardial cell damage. The purpose of this study was to test the hypothesis that the production of proinflammatory cytokines during cardiac surgery would be associated with myocardial dysfunction after the arterial switch operation in neonates. METHODS: A total of 63 neonates with transposition of the great arteries were operated on with combined deep hypothermic circulatory arrest and low-flow cardiopulmonary bypass at a median age of 7 days. Perioperative plasma concentrations of interleukins 6 and 8 were correlated with myocardial dysfunction, as assessed clinically and by echocardiography within 24 hours after the operation, and with perioperative cardiac troponin T blood levels as a marker of myocardial cell damage. RESULTS: Myocardial dysfunction was observed in 11 patients (17.5%), and 2 of them died. Durations of cardiopulmonary bypass and aortic crossclamping, but not of circulatory arrest, were correlated with myocardial dysfunction. Patients with myocardial dysfunction had significantly higher cardiac troponin T blood levels at the end of cardiopulmonary bypass and 4 and 24 hours after the operation than did patients without myocardial dysfunction. Patients with myocardial dysfunction also had higher interleukin 6 plasma concentrations after cardiopulmonary bypass and 4 hours after the operation, as well as higher interleukin 8 plasma concentrations 4 and 24 hours after the operation, than did those without myocardial dysfunction. Postoperative interleukin 6 and 8 plasma concentrations were significantly correlated with postoperative cardiac troponin T blood levels. Multivariable analysis of independent risk factors for myocardial dysfunction comprising cytokine and troponin levels and bypass duration revealed interleukin 6 levels 4 hours after the operation as significant (P =.047). CONCLUSIONS: Cardiac operations in neonates stimulate the production of proinflammatory cytokines, which may contribute to myocardial cell damage and myocardial dysfunction. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailHormonal and temperature responses to the 5-HT1a receptor agonist flesinoxan in normal volunteers
Pitchot, William ULg; Wauthy, Jacques ULg; Adam, Martine ULg et al

in Psychopharmacology (2002), 164(1), 27-32

RATIONALE: Flesinoxan is a highly potent and selective 5-HT(1A) agonist and appears to be a potentially interesting neuroendocrine serotonergic probe. OBJECTIVES: We assessed hormonal (ACTH, cortisol ... [more ▼]

RATIONALE: Flesinoxan is a highly potent and selective 5-HT(1A) agonist and appears to be a potentially interesting neuroendocrine serotonergic probe. OBJECTIVES: We assessed hormonal (ACTH, cortisol, prolactin and growth hormone) and temperature responses to flesinoxan in normal volunteers. METHODS: In a double-blind placebo-controlled study, single doses of 0.5 mg and 1 mg were injected over 10 min into 12 healthy male volunteers at 1-week intervals. Temperature and hormonal responses were measured at times -30, 0, 15, 30, 60, 90, and 120 min. RESULTS: Flesinoxan induced a significant and dose-dependent increase in adrenocorticotropic hormone (ACTH), cortisol, prolactin (PRL), growth hormone (GH) and a decrease in body temperature. Tolerance to flesinoxan was excellent. CONCLUSIONS: These results showed the role of 5-HT(1A) mechanisms in the PRL, ACTH, cortisol, GH, and temperature responses to flesinoxan. In the present study, flesinoxan appears a very promising serotonergic neuroendocrine probe. [less ▲]

Detailed reference viewed: 27 (3 ULg)
Full Text
Peer Reviewed
See detailDetermination of organohalogenated contaminants in liver of harbour porpoises (Phocoena phocoena) stranded on the Belgian North Sea coast
Covaci, Adrian; Van de Vijver, Kristin Inneke; De Coen, Wim et al

in Marine Pollution Bulletin (2002), 44(10),

Detailed reference viewed: 40 (15 ULg)
Full Text
Peer Reviewed
See detailThe transsexual : what about the future ?
Michel, Aude; Ansseau, Marc ULg; Legros, Jean-Jacques ULg et al

in European Psychiatry (2002), 17(6), 353-362

Since the 1950's, sexual surgical reassignments have been frequently carried out. As this surgical therapeutic procedure is controversial, it seems important to explore the actual consequences of such an ... [more ▼]

Since the 1950's, sexual surgical reassignments have been frequently carried out. As this surgical therapeutic procedure is controversial, it seems important to explore the actual consequences of such an intervention and objectively evaluate its relevance. In this context, we have carried out a review of the literature. After looking at the methodological limitations of follow-up studies, the psychological, sexual, social and profesionnal futures of the individuals subject to a transsexual operation are presented. Finally, prognostic aspects are considered. In the literature, follow-up studies tend to show that surgical transformations have positive consequences for the subjects. In the majority of cases, transsexuals are very satisfied with their intervention and any difficulties experienced are often temporary and disappear within a year after the surgical transformation. Studies show that there is less than 1% of regrets and a little more than 1% of suicides among operated subjects. The empirical research does not confirm the opinion that suicide is strongly associated with surgical transformation. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailLa question de l’alignement stratégique dans le cadre des nouvelles formes organisationnelles
Pichault, François ULg

in Revue de Gestion des Ressources Humaines (2002), (46), 59-75

Discusses the validity of the strategic alignment thesis when considering network organizations

Detailed reference viewed: 205 (38 ULg)
Full Text
Peer Reviewed
See detailKarst in granitic rocks, South Cameroon: cave genesis and silica and taranakite speleothems
Willems, Luc ULg; Compère, Philippe ULg; Hatert, Frédéric ULg et al

in Terra Nova (2002), 14(5), 355-362

A cave in granitic rocks was studied in Mezesse, South Cameroon. Coralloid speleothems, draperies and dissolution traces on the cave walls attest to its truly karstic nature. The speleothems consist of ... [more ▼]

A cave in granitic rocks was studied in Mezesse, South Cameroon. Coralloid speleothems, draperies and dissolution traces on the cave walls attest to its truly karstic nature. The speleothems consist of microlayers of opal and taranakite (K,NH4)Al-3(PO4)(3)(OH).9H(2)O. They indicate a significant mobilization of silica, Al and K from granite during the formation of the cave. Identification of silicified bacteria in the speleothems layers suggests a possible role of these micro-organisms in silica deposition. The presence of taranakite and of silicified organic remains within the speleothems lead to a better understanding of the genesis of the cave. [less ▲]

Detailed reference viewed: 154 (26 ULg)
Full Text
See detailL’accord sur le renouveau politique – Acte I : la réforme électorale
Rosoux, Géraldine ULg

in Journal du Juriste (2002), 14

Cet article présente le premier volet de l'accord du "renouveau politique", à savoir le volet portant sur la réforme électorale de 2002.

Detailed reference viewed: 80 (6 ULg)
Full Text
Peer Reviewed
See detailLa loi du 26 mai 2002 concernant le droit à l'intégration sociale
Michiels, Olivier ULg

in Journal du Juriste (2002), 14

L'article fait une très brève analyse de l'entrée en vigueur de la loi du 26 mai 2002 concernant le droit à l'intégration sociale

Detailed reference viewed: 65 (0 ULg)
Full Text
Peer Reviewed
See detailA novel family 8 xylanase, functional and physicochemical characterization
Collins, T.; Meuwis, Marie-Alice ULg; Stals, I. et al

in Journal of Biological Chemistry (2002), 277(38), 35133-35139

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailDécret d'optimalisation du CWATUP
Delnoy, Michel ULg

in Immobilier (2002), 16

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailPhase equivalent chains of Darboux transformations in scattering theory
Samsonov, B. F.; Stancu, Floarea ULg

in Physical Review C (2002), 66(3), 034001

We propose a procedure based on phase equivalent chains of Darboux transformations to generate local potentials satisfying the radial Schrodinger equation and sharing the same scattering data. For ... [more ▼]

We propose a procedure based on phase equivalent chains of Darboux transformations to generate local potentials satisfying the radial Schrodinger equation and sharing the same scattering data. For potentials related by a chain of transformations, an analytic expression is derived for the Jost function. It is shown how the same system of S-matrix poles can be differently distributed between poles and zeros of a Jost function that corresponds to different potentials with equal phase shifts. The concept of shallow and deep phase equivalent potentials is analyzed in connection with distinct distributions of poles. It is shown that phase equivalent chains do not violate the Levinson theorem. The method is applied to derive a shallow and a family of deep phase equivalent potentials describing the S-1(0) partial wave of the nucleon-nucleon scattering. [less ▲]

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailHuman immune cells express ppMCH mRNA and functional MCHR1 receptor
Verlaet, Myriam ULg; Adamantidis, Antoine ULg; Coumans, Bernard ULg et al

in FEBS Letters (2002), 527(1-3), 205-210

Melanin-concentrating hormone (MCH) is highly expressed in the brain and modulates feeding behavior. It is also expressed in some peripheral tissues where its role remains unknown. We have investigated ... [more ▼]

Melanin-concentrating hormone (MCH) is highly expressed in the brain and modulates feeding behavior. It is also expressed in some peripheral tissues where its role remains unknown. We have investigated MCH function in human and mouse immune cells. RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells. FACS and in vitro assay studies demonstrated that MCHR1 receptor expression on most cell types can trigger, in the presence of MCH, cAMP synthesis and calcium mobilization in peripheral blood mononuclear cells (PBMCs). Moreover, MCH treatment decreases the CD3-stimulated PBMC proliferation in vitro. Accordingly, our data indicate for the first time that MCH and MCHR1 may exert immunomodulatory functions. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 82 (6 ULg)
Full Text
Peer Reviewed
See detailControlled nitroxide-mediated radical polymerization of styrene, styrene/acrylonitrile mixtures, and dienes using a nitrone
Detrembleur, Christophe ULg; Sciannamea, Valérie; Koulic, Christian et al

in Macromolecules (2002), 35(19), 7214-7223

Radical polymerization of styrene and copolymerization of styrene and acrylonitrile (60/40) are controlled when conducted in the presence of N-tert-butyl-alpha-isopropylnitrone, which is easily ... [more ▼]

Radical polymerization of styrene and copolymerization of styrene and acrylonitrile (60/40) are controlled when conducted in the presence of N-tert-butyl-alpha-isopropylnitrone, which is easily synthesized from cheap reagents. However, for the control to be effective, the nitrone has to be prereacted with the radical initiator. Nitroxides are then formed "in situ", such that this nitrone system is an attractive alternative for the classical nitroxide-mediated polymerization (NMP), which may require a multistep synthesis of nitroxides or alkoxyamines. The choice of the radical initiator is important because it dictates the structure of the nitroxide and thus its capacity to control the radical polymerization. Well-defined poly(styrene)-b-poly(styrene-co-acrylonitrile), poly(styrene)-b-poly(n-butyl acrylate), and poly(styrene)-b-poly(isoprene) copolymers have been successfully synthesized by this process. [less ▲]

Detailed reference viewed: 73 (4 ULg)
Full Text
Peer Reviewed
See detailSusceptibility of trypanotolerant West African Dwarf goats and F1 crosses with the susceptible Sahelian breed to experimental Trypanosoma congolense infection and interactions with helminth infections and different levels of diet
Faye, D.; Osaer, S.; Goossens, B. et al

in Veterinary Parasitology (2002), 108(2), 117-136

dForty pure West African Dwarf (WAD) goats and 35 of its F1 crosses with the Sahelian breed were used in a multifactorial experimental design to evaluate the effects of an experimental Trypanosoma ... [more ▼]

dForty pure West African Dwarf (WAD) goats and 35 of its F1 crosses with the Sahelian breed were used in a multifactorial experimental design to evaluate the effects of an experimental Trypanosoma congolense infection and interactions with natural helminth infections and different levels of diet on health and productivity of these two breeds. Trypanosome infection caused a severe drop in packed cell volume (PCV), but this was not significantly affected by breed. Neither deworming nor diet had any effect on the course of anaemia after trypanosome infection. The mean score of parasitaemia tended to be higher in crossbreeds than in WAD goats although this was not significant (P > 0.05). Similarly, the antibody response to trypanosome infection was not significantly different between breeds. Parasitaemia level was significantly influenced by the level of diet with the group under high supplementation having a higher mean parasitaemia score than the group under low supplementation. Weight loss due to trypanosome infection tended to be greater in crossbreeds than in WAD goats (P > 0.05). During this study, there was no difference in mean helminth egg output between crossbred and WAD goats. However, between weeks 4 and 10 after trypanosome infection (corresponding to a period of heavy rainfall and highly infective pastures), the mean egg output was higher in the crossbreeds. The immunosuppressive effect of trypanosome infections was revealed by a lower antibody response to Haemonchus contortus in infected animals compared to the uninfected controls.Trypanosome infection tended to increase strongyle egg output. This study did no reveal any superior trypanotolerance of WAD goats compared to crossbreeds. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailFinite element simulation of springback in sheet metal forming
Papeleux, Luc ULg; Ponthot, Jean-Philippe ULg

in Journal of Materials Processing Technology (2002), 125(Sp. Iss. SI), 785-791

Although finite element analysis (FEA) is successful in simulating complex industrial sheet forming operations, the accurate and reliable application of this technique to springback has not been widely ... [more ▼]

Although finite element analysis (FEA) is successful in simulating complex industrial sheet forming operations, the accurate and reliable application of this technique to springback has not been widely demonstrated. Several physical parameters, as well as numerical, influence this phenomenon and its numerical prediction. In this paper, we investigate the impact of these parameters on the springback appearing in a 2D U-draw bending. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 61 (9 ULg)
Full Text
Peer Reviewed
See detailNumerical Simulation of Lubricated Contact in Rolling Processes
Boman, Romain ULg; Ponthot, Jean-Philippe ULg

in Journal of Materials Processing Technology (2002), 125-126

In this paper, the lubrication problem in numerical simulation of rolling process is presented. In this case, the recent and complex model of Marsault for the solution of the mixed lubrication regime has ... [more ▼]

In this paper, the lubrication problem in numerical simulation of rolling process is presented. In this case, the recent and complex model of Marsault for the solution of the mixed lubrication regime has been implemented and tested. This model requires the use of the finite difference method to work properly. We will discuss the advantages and the difficulties encountered when trying to solve the same problem with the finite element method in a general frame. Finally, a finite element formulation for the solution of the time-dependent Reynolds' equation coupled with the deformation of the workpiece is proposed. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 125 (10 ULg)
Full Text
See detailBulletin agrométéorologique - Août 2002
Tychon, Bernard ULg; Ozer, Pierre ULg; Eerens, H. et al

in Bulletin Agrométéorologique (2002)

Le mois d’août a été caractérisé par des précipitations fortement excédentaires. Les températures et l’humidité furent anormalement élevées, alors que la vitesse moyenne du vent fut exceptionnellement ... [more ▼]

Le mois d’août a été caractérisé par des précipitations fortement excédentaires. Les températures et l’humidité furent anormalement élevées, alors que la vitesse moyenne du vent fut exceptionnellement faible et le rayonnement médiocre sur l’ensemble du pays. Les données de terrain disponibles confirment la tendance des prévisions de rendement du bulletin précédent (juillet 2002). La situation des cultures est globalement favorable, avec des rendements qui s’annoncent supérieurs voire similaires à ceux de 2001 à l’exception du colza. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailFast automated extraction and clean-up of biological fluids for polychlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls analysis
Focant, Jean-François ULg; De Pauw, Edwin ULg

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2002), 776(2), 199-212

A fast automated extraction and clean-up procedure for low-level analysis of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and coplanar polychlorinated biphenyls (cPCBs ... [more ▼]

A fast automated extraction and clean-up procedure for low-level analysis of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and coplanar polychlorinated biphenyls (cPCBs) in biological fluids is presented. Online extraction of prepared fluids is carried out using disposable octadecyl bonded (C-18) solid-phase extraction columns. Extracts are then cleaned up through disposable multi-layer silica (acidic, basic and neutral) and dispersed PX-21 carbon columns. This new methodology is compared with classical Soxhlet extraction and manual solid-phase extraction in terms of repeatability, reproducibility, accuracy and recovery rates for reference and certified materials. Robustness is evaluated on different matrices, such as cow's milk, breast milk and human serum. As a consequence of the reduced number of reusable glassware used, as well as lowering of solvent consumption, recorded blank levels are decreased in favor of limits of detection (LODs). Total analysis time and cost are further reduced using simultaneous sample preparation units and the sample throughput is increased compared to classical methods. As a result, this new approach appears to be suitable for the fast sample preparation often required for such fluids in case of emergency foodstuffs analysis or during large epidemiological studies. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 36 (9 ULg)
Full Text
Peer Reviewed
See detailThe Quadruple Wolf-Rayet System GP Cephei: Spectral Types, Masses, Mass-Loss Rate, and Colliding Winds
Demers, H.; Moffat, A. F. J.; Marchenko, S. V. et al

in Astrophysical Journal (2002), 577

We have reevaluated the orbital elements for each pair of the quadruple (W-R+O) + (O+O) stellar system GP Cep and propose new spectral types WN6o/WCE + O3-6, B0: I + B1: V-III. It is shown that there is ... [more ▼]

We have reevaluated the orbital elements for each pair of the quadruple (W-R+O) + (O+O) stellar system GP Cep and propose new spectral types WN6o/WCE + O3-6, B0: I + B1: V-III. It is shown that there is only one Wolf-Rayet (W-R) star in GP Cep, contrary to a previous claim. A rate of change P=1.3+/-0.2 s yr[SUP]-1[/SUP] is determined for the W-R+O pair, which leads to a new period of 6.6887 days and to a W-R mass-loss rate of (0.8-3.0)à 10[SUP]-5[/SUP] M[SUB]solar[/SUB] yr[SUP]-1[/SUP]. Masses for this pair are estimated to be M[SUB]W-R[/SUB]>~6 M[SUB]solar[/SUB] and M[SUB]O[/SUB]>~21 M[SUB]solar[/SUB]. The effects of wind-wind collision in the W-R+O pair are studied. It is shown that even after allowing for dilution by the OB components of the quadruple system, these effects are not as strong as in the binary V444 Cygni (WN5+O6, P=4.212 days). In GP Cep, the phase-dependent, relatively weak excess emission does not originate in the arms of the bow shock cone. Rather, it emerges from the extra heated portion of the W-R wind facing the hot O companion. The trailing bow shock arm is clearly seen, however, as an enhanced He I absorption component near quadrature at phase ~0.73. An anomalous blueshifted He I absorption is present at phase ~0.9, as is also seen in V444 Cyg, in the WC8+O9 I/O8 III binary γ Velorum and in the LBV-cotype binary R81 (B2.5 Iab:e). A 3.5 day orbit for the eclipsing B star pair is confirmed. [less ▲]

Detailed reference viewed: 32 (1 ULg)
Full Text
Peer Reviewed
See detailInterplanetary magnetic field control of afternoon-sector detached proton auroral arcs
Burch, J. L.; Lewis, W. S.; Immel, T. J. et al

in Journal of Geophysical Research. Space Physics (2002), 107

Data from the Far Ultraviolet Imager (FUV) on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) satellite show that subauroral proton arcs appear in the afternoon sector during ... [more ▼]

Data from the Far Ultraviolet Imager (FUV) on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) satellite show that subauroral proton arcs appear in the afternoon sector during geomagnetically disturbed periods when the interplanetary magnetic field rotates either from south to north or from west to east and when the magnetosphere is moderately compressed. Time series of proton aurora images show that the proton emissions are generally aligned along the equatorward part of the auroral oval. However, when interplanetary magnetic field (IMF) B[SUB]z[/SUB] changes from negative to positive the auroral oval contracts toward higher latitudes while the ring current proton precipitation remains stationary, resulting in a separation of several degrees between the latitude of the new oval position and a subauroral proton arc in the afternoon sector. A similar effect occurs when IMF B[SUB]y[/SUB] rotates from negative to positive, in which case the oval in the afternoon sector retreats toward higher latitudes, again leaving a separation between the oval and the subauroral proton arc of several degrees. Comparisons with low-altitude and geosynchronous satellite data show that the subauroral proton arc is caused by the precipitation of protons with energies from several keV to 30 keV and is likely associated with the existence of a plasmaspheric ``drainage plume.'' In contrast, the proton emissions along the main oval are caused by protons with energies generally less than 10 keV. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailSynthesis and compatibilization ability of hydrogenated polybutadiene-b-polyamide 6 diblock copolymer in low density polyethylene and polyamide 6 blends
Harrats, Charef; Fayt, R.; Jérôme, Robert ULg

in Polymer (2002), 43(19), 5347-5354

This paper describes the synthesis of hydrogenated polybutadiene-b-polyamide 6 (HPB-b-PA6), a pure diblock copolymer, which consists of three main steps: synthesis of hydroxyl terminated polybutadiene ... [more ▼]

This paper describes the synthesis of hydrogenated polybutadiene-b-polyamide 6 (HPB-b-PA6), a pure diblock copolymer, which consists of three main steps: synthesis of hydroxyl terminated polybutadiene, its hydrogenation and functionalization with an epsilon-caprolactam blocked diisocyanate. In a third step the functionalized HPB is copolymerized with epsilon-caprolactam monomer via ring opening anionic polymerization. The compatibilization ability of the synthesized pure diblock copolymer was evaluated in low-density polyethylene (LDPE)/polyamide 6 (PA6) blends. The investigation includes phase morphology development using both optical and electron scanning microscopy. The ultimate tensile properties of the modified and non-modified blends were also evaluated. The synthesized pure diblock copolymer exhibits very interesting interfacial activity both in terms of particle size reduction and improvement of the interfacial adhesion between the incompatible LDPE/PA6 phases. The tensile properties of the investigated blends were also significantly affected by the addition of the copolymer. The efficiency of compatibilization was found to be very sensitive to the molecular characteristics such as composition and molecular weight of the copolymer. Among the copolymers investigated a copolymer containing 24 wt% PA6 and having a total molecular weight (M) over bar (a) of 87,000 was found to exhibit the most efficient interfacial activity. [less ▲]

Detailed reference viewed: 61 (1 ULg)
Full Text
Peer Reviewed
See detailDevelopment and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass (R) for tissue engineering applications
Roether, J. A.; Boccaccini, Aldo R.; Hench, L. L. et al

in Biomaterials (2002), 23(18), 3871-3878

Bioactive and bioresorbable composite materials were fabricated using macroporous poly(DL-lactide) (PDLLA) foams coated with and impregnated by bioactive glass (Bioglass®) particles. Stable and ... [more ▼]

Bioactive and bioresorbable composite materials were fabricated using macroporous poly(DL-lactide) (PDLLA) foams coated with and impregnated by bioactive glass (Bioglass®) particles. Stable and homogeneous Bioglasss coatings on the surface of PDLLA foams as well as infiltration of Bioglass® particles throughout the porous network were achieved using a slurry-dipping technique in conjunction with pre-treatment of the foams in ethanol. The quality of the bioactive glass coatings was reproducible in terms of thickness and microstructure. Additionally, electrophoretic deposition was investigated as an alternative method for the fabrication of PDLLA foam/Bioglass® composite materials. In vitro studies in simulated body fluid (SBF) were performed to study the formation of hydroxyapatite (HA) on the surface of PDLLA/Bioglass® composites. SEM analysis showed that the HA layer thickness rapidly increased with increasing time in SBF. The high bioactivity of the PDLLA foam/Bioglasss composites indicates the potential of the materials for use as bioactive, resorbable scaffolds in bone tissue engineering. [less ▲]

Detailed reference viewed: 103 (3 ULg)
Full Text
See detailProposition d'une méthode d'inventaire des dégâts frais de cervidés applicable en Région wallonne : les dégâts d'écorcement
Lejeune, Philippe ULg; Rotheudt, H.; Verrue, V.

in Forêt Wallonne (2002), (60), 4-10

Detailed reference viewed: 25 (9 ULg)
Full Text
Peer Reviewed
See detailUsefulness of F-18-FDG PET in the post-therapy surveillance of endometrial carcinoma
Belhocine, Tarik; De Barsy, Caroline; Hustinx, Roland ULg et al

in European Journal of Nuclear Medicine and Molecular Imaging (2002), 29(9), 1132-1139

The aim of this study was to assess the usefulness of fluorine-18 tluoro-2-deoxy-D-glucose positron emission tomography (FDG PET) in the post-therapy surveillance of endometrial carcinomas. Forty-one ... [more ▼]

The aim of this study was to assess the usefulness of fluorine-18 tluoro-2-deoxy-D-glucose positron emission tomography (FDG PET) in the post-therapy surveillance of endometrial carcinomas. Forty-one fully corrected whole-body PET studies were performed in 34 women with previously treated endometrial cancers as a part of their follow-up programme. In 28 studies, FDG PET was indicated to localise a recurrence suspected at the control visits on the basis of clinical examination and/or radiological abnormalities (chest X-ray, CT or MRI) and/or elevated tumour marker levels (CA125, CEA). Another 13 studies were performed as a simple surveillance procedure. Overall, in 26 studies PET detected recurrent disease, which was confirmed either by histology (n=7) or by clinical and radiological outcomes (n=19). In 88% of the cases, the PET findings confirmed recurrence suggested by routine follow-up. In the remaining 12% of cases, PET detected asymptomatic recurrences that were unsuspected at the control visits. Whole-body PET accurately localised the site of confirmed recurrences as being above and below the diaphragm in 50%, only below the diaphragm in 35% and only above the diaphragm in 15%. In one patient, however, PET missed microscopic lung metastases shown on thoracic CT, and in three studies, metabolic imaging results were not confirmed. In I I of 12 negative PET studies, no subsequent clinical or radiological recurrences were observed with a median follow-up of 10 months. Overall, the results of PET agreed well with the final diagnosis (Cohen's kappa coefficient =0.78). In 9/26 patients (35%) with confirmed recurrences, the PET findings significantly altered the treatment choice by detecting either clinically or radiologically unsuspected distant metastases. The sensitivity, specificity, diagnostic accuracy and positive and negative predictive values of FDG PET imaging in the post-therapy surveillance of endometrial carcinomas were 96%, 78%, 90%, 89% and 91 %, respectively. Indeed, the high likelihood ratio for a positive test result (4.5) and the low likelihood ratio for a negative test result (0.05) demonstrated the clinical utility of metabolic imaging in "ruling in" disease as well as "ruling out" recurrence. In conclusion, whole-body FDG PET appears useful in the post-therapy surveillance of endometrial cancers, both for the accurate localisation of suspected recurrences and for the detection of asymptomatic recurrent disease. [less ▲]

Detailed reference viewed: 498 (0 ULg)
Full Text
Peer Reviewed
See detailMethods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation
Efthimiadis, A.; Spanevello, A.; Hamid, Q. et al

in European Respiratory Journal (2002), 20(Suppl. 37), 19-23

Detailed reference viewed: 25 (4 ULg)
Full Text
Peer Reviewed
See detailSexual partner preference requires a functional aromatase (Cyp19) gene in male mice
Bakker, Julie ULg; Honda, S.; Harada, N. et al

in Hormones & Behavior (2002), 42(2), 158-171

Sexual motivation, sexual partner preference, and sexual performance represent three different aspects of sexual behavior that are critical in determining the reproductive success of a species. Although ... [more ▼]

Sexual motivation, sexual partner preference, and sexual performance represent three different aspects of sexual behavior that are critical in determining the reproductive success of a species. Although the display of sexual behavior is under strict hormonal control in both sexes, the relative roles of androgen and estrogen receptors in activating the various components of male sexual behavior are still largely unknown. A recently developed mouse model that is deficient in estradiol due to targeted disruption of exons 1 and 2 of the Cyp19 gene (aromatase knockout (ArKO) mice) was used here to analyze the role of estradiol in the control of all three aspects of male sexual behavior. When tested in a Y-maze providing volatile olfactory cues, male ArKO mice did not show a preference for the odors from an estrous female over those from an intact male, whereas wildtype (WT) and heterozygous (HET) males clearly preferred to sniff estrous odors. When provided with visual and olfactory cues, male ArKO mice also failed to show a preference for an estrous female when given a choice between an estrous female and an empty arm. However, sexual partner preferences of male ArKO mice were not sex-reversed: they did not prefer to investigate an intact male over an estrous female or empty arm. Thus, male ArKO mice seemed to have general deficits in discriminating between conspecifics by using olfactory and visual cues. Male coital behavior was also severely impaired in male ArKO mice: they displayed significantly fewer mounts, intromissions, and ejaculations than WT and HET males. Latencies to first mount or intromission were also significantly longer in ArKO males compared to WT and HET males, in addition to them showing less interest in investigating olfactory and visual cues in a Y-maze, suggesting that they were sexually less motivated. However, three out of seven male ArKO mice were capable of siring litters provided they were housed with a female for a prolonged period of time. In conclusion, aromatization of testosterone to estradiol appears to be essential for sexual motivation and sexual partner preference. By contrast, estradiol may play only a limited role in the expression of male coital behaviors. (C) 2002 Elsevier Science (USA). [less ▲]

Detailed reference viewed: 33 (0 ULg)
Full Text
Peer Reviewed
See detailTumor necrosis factor-alpha and troponin I release in porcine cardiac lymph and coronary sinus blood before and after cardiopulmonary bypass.
Vazquez-Jimenez, J. F.; Liakopoulos, O. J.; Qing, Ma et al

in Lymphology (2002), 35(3), 105-13

To assess the concentrations of cardiac troponin I (cTnI) and tumor necrosis factor-alpha (TNFalpha) in cardiac lymph compared with coronary sinus (CS) blood and to measure cardiac lymph flow before and ... [more ▼]

To assess the concentrations of cardiac troponin I (cTnI) and tumor necrosis factor-alpha (TNFalpha) in cardiac lymph compared with coronary sinus (CS) blood and to measure cardiac lymph flow before and after cardiopulmonary bypass (CPB). In 21 pigs, the main cardiac lymph trunk was cannulated before institution of standardized CPB. Lymph flow, cTnI and TNFa in cardiac lymph and CS blood were measured before and after CPB for 6 hours. Before CPB, cTnI concentration was 215 +/- 36 nglml in cardiac lymph and 0.5 +/- 0.1 nglml in CS blood, respectively. After aortic declamping a significant elevation of cTnI values was measured in cardiac lymph and CS blood. cTnl concentration in cardiac lymph and CS blood peaked 6 hrs after CPB (10,556 +/- 4,735 vs. 22.2 +/- 3.7 nglml, p < 0.01). TNFalpha concentration at baseline was 23.2 +/- 5.6 pg/ml in lymph and 18.7 +/- 9.5 pg/ml in CS blood, and there was no significant release of TNFalpha up to the end of the experiment. Baseline cardiac lymph flow was 3.07 +/- 0.35 ml/h and declined after aortic clamping (0.72 +/- 0.16 ml/h; p < 0.01) and peaked one hour after CPB (5.66 +/- 0.97 ml/h; p < 0.01). In conclusion, very high cTnI concentrations in cardiac lymph suggest serious perioperative myocardial damage after CPB with cardioplegia, which is underestimated by cTnI release into the bloodstream. In our study, the myocardium was not a major source of TNFalpha release. [less ▲]

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailFamilial colloid cyst of the third ventricle: neuroendocrinological follow-up and review of the literature.
Valdes Socin, Hernan Gonzalo ULg; Born, J.; Wallemacq, Caroline et al

in Clinical Neurology & Neurosurgery (2002), 104(4), 367-370

Colloid cysts of the third ventricle are rare, benign cysts of endodermal origin. Between 1989 and 1999, eight patients with this lesion (five females, three males), with a mean age of 40.5 years (range ... [more ▼]

Colloid cysts of the third ventricle are rare, benign cysts of endodermal origin. Between 1989 and 1999, eight patients with this lesion (five females, three males), with a mean age of 40.5 years (range 20-54), were identified out of 1354 operated for tumours of the central nervous system. Among the eight, two were familial. They were half sisters 38 and 28 years-old, who were diagnosed to have colloid cysts of the third ventricle on CT scanning. Transcortical excision yielded 10 and 15 mm sized colloid cysts, respectively. Moreover, both sisters developed a multinodular goiter associated with these congenital tumours. The second sibling developed hyperprolactinemia associated with macroprolactinemia. Pregnancy was only possible after bromocriptine treatment. These cases provide further evidences that colloid cysts probably have an autosomic recessive pattern of inheritance with variable penetrance. [less ▲]

Detailed reference viewed: 36 (1 ULg)
Full Text
Peer Reviewed
See detailIncreased uptake of the apoptosis-imaging agent (99m)Tc recombinant human annexin V in human tumors after one course of chemotherapy as a predictor of tumor response and patient prognosis
Belhocine, Tarik; Steinmetz, Neil; Hustinx, Roland ULg et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (2002), 8(9), 2766-2774

Purpose: Many anticancer therapies exert their therapeutic effect by inducing apoptosis in target tumors. We evaluated in a Phase I study the safety and the feasibility of Tc-99m-Annexin V for imaging ... [more ▼]

Purpose: Many anticancer therapies exert their therapeutic effect by inducing apoptosis in target tumors. We evaluated in a Phase I study the safety and the feasibility of Tc-99m-Annexin V for imaging chemotherapy-induced apoptosis in human cancers immediately after the first course of chemotherapy. Experimental Design: Fifteen patients presenting with lung cancer (n = 10), lymphoma (n = 3), or breast cancer (n = 2) underwent Tc-99m-Annexin V scintigraphy before and within 3 days after their first course of chemotherapy. Tumor response was evaluated by computed tomography and F-18-fluoro-2-deoxy-D-glucose positron emission tomography scans, 3 months in average after completing the treatment. Median follow-up was 117 days. Results: In all cases, no tracer uptake was observed before treatment. However, 24-48 h after the first course of chemotherapy, 7 patients who showed Tc-99m-Annexin V uptake at tumor sites, suggesting apoptosis, had a complete (n = 4) or a partial response In = 3). Conversely, 6 of the 8 patients who showed no significant posttreatment tumor uptake had a progressive disease. Despite the lack of tracer uptake after treatment, the 2 patients with breast cancer had a partial response. Overall survival and progression-free survival were significantly related to tracer uptake in treated lung cancers and lymphomas (P < 0.05). No serious adverse events were observed. Conclusions: Our preliminary results demonstrated the feasibility and the safety of Tc-99m-Annexin V for imaging apoptosis in human tumors after the first course of chemotherapy. Initial data suggest that early Tc-99m-Annexin V tumor uptake may be a predictor of response to treatment in-patients with late stage lung cancer and lymphoma. [less ▲]

Detailed reference viewed: 46 (5 ULg)
Full Text
Peer Reviewed
See detailFetal infection with Neospora caninum in dairy and beef cattle in Belgium
De Meerschman, F.; Speybroeck, N.; Berkvens, D. et al

in Theriogenology (2002), 58(5), 933-945

Neospora caninum is a protozoan parasite, which causes fetal and neonatal mortality in livestock and companion animals. In 224 abortions in Belgian cattle, different diagnostic methods were used to ... [more ▼]

Neospora caninum is a protozoan parasite, which causes fetal and neonatal mortality in livestock and companion animals. In 224 abortions in Belgian cattle, different diagnostic methods were used to demonstrate infection, and the presence of N. caninum. An indirect fluorescent antibody test (IFAT) was used to analyze fetal and maternal sera and immunohistochemistry (IHC) was performed when lesions consistent with neosporosis were observed in the brain, heart or liver. Twenty dairy cattle sera out of 70 (29%) and 13 beef cattle sera out of 93 (14%) were positive by IFAT. A positive titer to N. caninum was found in seven and three fetuses born to beef and dairy cows, respectively. Lesions consistent with N. caninum infection were observed in 17 fetuses. Of nine positive beef fetuses, five were confirmed by IHC while, all but one dairy fetus were confirmed using the same technique. Age had no influence on the serological status of the mother (P = 0.486) whereas husbandry system had a borderline influence (P = 0.082). However, a strong association (P = 0.004) between the level of antibodies in the darn and the occurrence of lesions in the fetus was observed and lesions were more prominent in dairy than in beef fetuses. Additionally, the distribution of intra-cerebral lesions was more extensive in dairy than in beef fetuses (P < 0.0001). Age and serological status of the fetus were found to influence the occurrence of lesions in beef fetuses (both P < 0.001) but no such significant relationships could be demonstrated in dairy fetuses. The study indicated that N. caninum must be considered as an important cause of bovine abortion in Belgium. (C) 2002 Elsevier Science Inc. All rights reserved. [less ▲]

Detailed reference viewed: 48 (4 ULg)
Full Text
See detailComme Janus aux deux visages, des écrivains aux deux publics
Delbrassine, Daniel ULg

in Revue des livres pour enfants (2002), 206

Evocation des premiers résultats d'une recherche comparative sur l'écriture des auteurs mixtes (publiés en littérature de jeunesse et en littérature générale)

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailBiodegradable polyester layered silicate nanocomposites based on poly(epsilon-caprolactone)
Pantoustier, Nadège; Lepoittevin, Bénédicte; Alexandre, Michaël ULg et al

in Polymer Engineering & Science (2002), 42(9), 1928-1937

Nanocomposites based on biodegradable poly(epsilon-caprolactone) (PCL) and layered silicates (montmorillonite, MMT) were prepared either by melt intercalation with PCL or by in situ ring-opening ... [more ▼]

Nanocomposites based on biodegradable poly(epsilon-caprolactone) (PCL) and layered silicates (montmorillonite, MMT) were prepared either by melt intercalation with PCL or by in situ ring-opening polymerization of epsilon-caprolactone as promoted by the so-called coordination-insertion mechanism. Both non-modified clays (Na+-MMT) and silicates modified by various alkylammonium cations were studied. Mechanical and thermal properties were examined by tensile testing and thermogravimetric analysis. Even at a filler content as low as 3 wt% of inorganic layered silicate, the PCL-layered silicate nanocomposites exhibited improved mechanical properties (higher Young's modulus) and increased thermal stability as well as enhanced flame retardant characteristics as a result of a charring effect. It was shown that the formation of PCL-based nanocomposites depended not only on the nature of the ammonium cation and related functionality but also on the selected synthetic route, melt intercalation vs. in situ intercalative polymerization. Interestingly enough, when the intercalative polymerization of epsilon-caprolactone was carried out in the presence of MMT organo-modified with ammonium cations bearing hydroxyl functions, nanocomposites with much improved mechanical properties were recovered. Those hybrid polyester layered silicate nanocomposites. were characterized by a covalent bonding between the polyester chains and the clay organo-surface as a result of the polymerization mechanism, which was actually initiated from the surface hydroxyl functions adequately activated by selected tin (II) or tin (IV) catalysts. [less ▲]

Detailed reference viewed: 112 (3 ULg)
Full Text
Peer Reviewed
See detailDemographic and clinical parameters influencing the short-term outcome of anti-tumor necrosis factor (infliximab) treatment in Crohn's disease
Vermeire, S.; Louis, Edouard ULg; Carbonez, A. et al

in American Journal of Gastroenterology (2002), 97(9), 2357-2363

OBJECTIVE: Infliximab is an effective treatment for refractory or fistulizing Crohn's disease (CD). However, about 30% of patients do not respond to infliximab for unknown reasons. Identifying predictive ... [more ▼]

OBJECTIVE: Infliximab is an effective treatment for refractory or fistulizing Crohn's disease (CD). However, about 30% of patients do not respond to infliximab for unknown reasons. Identifying predictive factors of response is important for optimizing clinical management and for better understanding infliximab's mechanisms of action. The aim of this study was to assess whether demographic or clinical parameters influence short-term response to infliximab. METHODS: The first 240 CD patients of the Belgian Infliximab Expanded Access Program were studied for response to infliximab treatment and assessed at 4 (refractory luminal CD) or 10 wk (fistulizing CD) after the first infusion. Detailed demographic and clinical information on age, sex, type of disease (fistulizing or refractory), Crohn's Disease Activity Index score, C-reactive protein (CRP), smoking habits, disease duration, localization of disease, concomitant medication, and previous surgery were obtained from all patients. Logistic regression and decision tree analysis were performed. RESULTS: There were 73.5% responders and 26.5% nonresponders to treatment. Stepwise logistic regression identified age (OR = 0.971, 95% CI = 0.947-0.995, p = 0.018), isolated ileitis (OR = 0.359, 95% CI 0.177-0.728, p = 0.004), and previous surgery (OR 0.429, 95% CI = 0.233-0.787, p = 0.006) as inversely correlated with response, whereas isolated colitis (OR = 1.905, 95% CI = 1.010-3.597, p = 0.046) and concomitant immunosuppressive treatment (OR = 2.670, 95% CI = 1.430-5.016, p = 0.0022) were positively correlated with response to infliximab. Surprisingly, smoking habits were not retained as predictors for response. Decision tree analysis provided a working algorithm based on age and immunosuppressive treatment that warrants further exploration. CONCLUSIONS: In this large cohort of infliximab-treated CD patients, young age, Crohn's colitis, and concomitant immunosuppressive treatment were identified as independent variables favoring short-term response to infliximab. [less ▲]

Detailed reference viewed: 49 (0 ULg)
Full Text
Peer Reviewed
See detailMethyl-laudanosine: A new pharmacological tool to investigate the function of small-conductance Ca2+-activated K+ channels
Scuvée-Moreau, Jacqueline ULg; Liégeois, Jean-François ULg; Massotte, Laurent ULg et al

in Journal of Pharmacology and Experimental Therapeutics (The) (2002), 302(3), 1176-1183

Small-conductance Ca(2+)-activated K(+) channels (SK channels) underlie the prolonged postspike afterhyperpolarization (AHP) observed in many central neurons and play an important role in modulating ... [more ▼]

Small-conductance Ca(2+)-activated K(+) channels (SK channels) underlie the prolonged postspike afterhyperpolarization (AHP) observed in many central neurons and play an important role in modulating neuronal activity. However, a lack of specific and reversible blockers of these channels hampers their study in various experimental conditions. Because previous work has shown that bicuculline salts block these channels, we examined whether related alkaloids, namely laudanosine quaternary derivatives, would produce similar effects. Intracellular recordings were performed on rat midbrain dopaminergic neurons and hippocampus CA1 pyramidal cells. Binding experiments were performed on rat cerebral cortex membranes. Laudanosine, methyl-laudanosine, and ethyl-laudanosine blocked the apamin-sensitive AHP of dopaminergic neurons with mean IC(50) values of 152, 15, and 47 microM, respectively. The benzyl and butyl derivatives were less potent. Methyl-laudanosine had no effect on the I(h) current, action potential parameters, or membrane resistance of dopaminergic cells, or on the decrease in input resistance induced by muscimol, indicating a lack of antagonism at GABA(A) receptors. Interestingly, 100 microM methyl-laudanosine induced a significant increase in spiking frequency of dopaminergic neurons but not of CA1 pyramidal cells, suggesting the possibility of regional selectivity. Binding experiments on laudanosine derivatives were in good agreement with electrophysiological data. Moreover, methyl-laudanosine has no affinity for voltage-gated potassium channels, and its affinity for SK channels (IC(50) 4 microM) is superior to its affinity for muscarinic (IC(50) 114 microM) and neuronal nicotinic (IC(50) > or =367 microM) receptors. Methyl-laudanosine may be a valuable pharmacological tool to investigate the role of SK channels in various experimental models. [less ▲]

Detailed reference viewed: 56 (20 ULg)
Full Text
Peer Reviewed
See detailAccelerated postoperative radiation therapy with weekly concomitant boost in high risk patients with squamous-cell carcinoma of the head and neck
Chevalier; Pasche; COUCKE, Philippe ULg et al

in Radiotherapy & Oncology (2002), 64(supp 1), 248

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailLa fenêtre implantatoire
PERRIER d'HAUTERIVE, Sophie ULg; Charlet-Renard, Jeanne de Chantal ULg; Goffin, Frédéric ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2002), 31(5), 440-55

The implantation window. Background. Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this ... [more ▼]

The implantation window. Background. Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this endometrium. Though implantation could occur in any human tissue, the endometrium is the only tissue where embryo implantation cannot occur except during a restricted period called the implantation window. During this window, the endometrium is highly receptive to the embryo. Material and methods. We reviewed the literature concerning the different factors involved in improved endometrial receptivity and implantation. Results. Maternal - embryo crosstalk is favored by the implantation window. Endometrial receptivity results from the acquisition of ligands or receptors facilitating apposition, then adhesion of the embryo, or from the loss of components preventing it. The molecular basis of the implantation window remains to be defined. Conclusion. Despite progress in assisted reproduction technologies, the lack of control of implantation remains a major obstacle to successful pregnancy. It is of prime importance to determine the characteristic features of a receptive endometrium and, among the many markers proposed by in vitro studies, to analyze in humans those demonstrated by knock-out experiments to play a crucial role in mice. [less ▲]

Detailed reference viewed: 110 (6 ULg)
Full Text
See detailComparaison de différents types de compostages de matières organiques diverses non valorisées par les paysans au Rwanda
Culot, Marc ULg; Mbonigaba Muhinda, Jean-Jacques; Maniraguha, J. D.

in Etudes Rwandaises : Série sciences exactes, naturelles et appliquées (2002), 5

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailDecreased local control following radiation therapy alone in early larynx cancer with anterior commisure extension
Ozsahin; Bron; COUCKE, Philippe ULg et al

in Radiotherapy & Oncology (2002), 64(supp 1), 243-244

Detailed reference viewed: 6 (0 ULg)
Full Text
See detailMaladie du hêtre. Bilan chiffré de la situation à l'été 2001
Rondeux, Jacques ULg; Bourland, Nils ULg; Huart, O. et al

in Forêt Wallonne (2002), (60), 2-3

Durant l'été 2001, un inventaire des dégâts occasionnés par la maladie du hêtre à été réalisé. Les résultats de cette étude ont été publiés dans la revue Silva belgica. Nous en reprenons ici la conclusion ... [more ▼]

Durant l'été 2001, un inventaire des dégâts occasionnés par la maladie du hêtre à été réalisé. Les résultats de cette étude ont été publiés dans la revue Silva belgica. Nous en reprenons ici la conclusion ainsi que quelques-uns des nombreux graphiques et tableaux qui y sont présentés. Nous invitons le lecteur intéressé à se référer à l'article original. [less ▲]

Detailed reference viewed: 37 (12 ULg)
Full Text
Peer Reviewed
See detailDécompensation cardiaque, fonction rénale et anti-inflammatoires non stéroïdiens
Krzesinski, Jean-Marie ULg; Piront, Patricia ULg

in Revue Médicale de Liège (2002), 57(9), 582-586

Thanks to a case report of heart failure in an old people with a cardiovascular history treated by the new coxib-inhibitors, we would like to remember and insist to the risk of renal and cardiac ... [more ▼]

Thanks to a case report of heart failure in an old people with a cardiovascular history treated by the new coxib-inhibitors, we would like to remember and insist to the risk of renal and cardiac complications which appear to be the same as those with the non specific antiinflammatory drugs. Old age, diuretic or converting enzyme inhibitor treatment, heart failure, liver insufficiency, nephrotic syndrome are risk factors for acute renal failure and cardiac failure during such treatment. [less ▲]

Detailed reference viewed: 1011 (8 ULg)
Full Text
Peer Reviewed
See detailRadiative lifetimes of 6sns S-3(1) and 6snd D-3(1) excited states of HgI
Blagoev, K.; Pentchev, V.; Biémont, Emile ULg et al

in Physical Review A (2002), 66(3),

Natural radiative lifetimes have been measured along the 6sns S-3(1) (n=7-10) and 6snd D-3(1) (n=6-11) Rydberg series of neutral mercury using time-resolved laser-induced fluorescence spectroscopy in a ... [more ▼]

Natural radiative lifetimes have been measured along the 6sns S-3(1) (n=7-10) and 6snd D-3(1) (n=6-11) Rydberg series of neutral mercury using time-resolved laser-induced fluorescence spectroscopy in a laser-produced mercury plasma. The states of Hg I investigated in the present work were populated from the ground state by a stepwise excitation process. Relativistic Hartree-Fock calculations, including intravalence interactions and core-polarization effects, have been performed and the theoretical lifetimes have been compared with the measurements. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailProgress in microscopic modeling of damage in steel at high temperature
Remy, M.; Castagne, Sylvie; Habraken, Anne ULg

in International Journal of Forming Processes (2002), 5(N°2-3-4/2002), 445-455

With the goal to study crack propagation in continuous casting, this paper presents an on going project to develop and identify a mathematical finite element model of damage in steel at high temperature ... [more ▼]

With the goal to study crack propagation in continuous casting, this paper presents an on going project to develop and identify a mathematical finite element model of damage in steel at high temperature. A representative microscopic cell of the material is presented and adapted to available chemical information, precipitation state and micro-structures observed using metallographic as weel as imaging methods (optical microscopy, SEM, orientation imaging microscopy). Compression tests of cylindrical samples after a thermal treatment have been performed to identify the parameters of an elasto-visco-plastique law of Norton-Hoff type. Hot damage tests in tensile and compression states help to provide information on crack appearance. [less ▲]

Detailed reference viewed: 79 (5 ULg)
Full Text
Peer Reviewed
See detailRelationships between inner ear and sagitta growth during ontogenesis of three Carapini species, and consequences of life-history events on the otolith microstructure
Parmentier, Eric ULg; Lagardère, Françoise; Vandewalle, Pierre ULg

in Marine Biology (2002), 141(3), 491-501

Three species of Carapidae have in common a tenuis larval stage, during which they settle in the lagoon and take refuge in the same species of holothurold. From the juvenile stage, Carapus homei and C ... [more ▼]

Three species of Carapidae have in common a tenuis larval stage, during which they settle in the lagoon and take refuge in the same species of holothurold. From the juvenile stage, Carapus homei and C. boraborensis are commensal, whereas Encheliophis gracilis is parasitic. The aims of this study were to analyse to what extent the ontogenctic changes of the otic capsule affected the shape of the inner ear and how environmental cues, due to the above-mentioned life history and the style, could influence the structure of the sagitta. Sagittal sections revealed a three-dimensional asymmetry with a nucleus close to the proximal surface. Observations of the growth axis of the sagitta suggest it has a morphogenetic impact on the otic cavity. Each sagitta contains three main zones related to the life stages of the fish. Bands and checks were observed in the third zone in C. homei and C. boraborensis, but this pattern was less discernible in E. gracilis. These structural differences in zone 3 could be related to the commensal and parasitic life styles of these fishes. Further studies dealing with otosac features and otolith functions are suggested. [less ▲]

Detailed reference viewed: 56 (4 ULg)
Full Text
Peer Reviewed
See detailIdentifying environmental risk factors for endemic cholera: a raster GIS approach
Ali, M.; Emch, M.; Donnay, Jean-Paul ULg et al

in Health & Place (2002), 8(3), 201-210

The bacteria that cause cholera are known to be normal inhabitants of surface water, however, the environmental risk factors for different biotypes of cholera are not well understood. This study ... [more ▼]

The bacteria that cause cholera are known to be normal inhabitants of surface water, however, the environmental risk factors for different biotypes of cholera are not well understood. This study identifies environmental risk factors for cholera in an endemic area of Bangladesh using a geographic information systems (GIS) approach. The study data were collected from a longitudinal health and demographic surveillance system and the data were integrated within a geographic information system database of the research area. Two study periods were chosen because they had different dominant biotypes of the disease. From 1992 to 1996 El Tor cholera was dominant and from 1983 to 1987 classical cholera was dominant. The study found the same three risk factors for the two biotypes of cholera including proximity to surface water, high population density, and poor educational level. The GIS database was used to measure the risk factors and spatial filtering techniques were employed. These robust spatial methods are offered as an example for future epidemiological research efforts that define environmental risk factors for infectious diseases. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 106 (2 ULg)
Peer Reviewed
See detailEchocardiographie dynamique dans l'insuffisance mitrale
Lancellotti, Patrizio ULg; Pierard, L. A.

in Archives des Maladies du Coeur et des Vaisseaux (2002), 95(9), 793-8

Mitral insufficiency can have a dynamic character, correlating to an intermittent increase in the area of the regurgitant orifice and the regurgitant volume. Among the various stress methods ... [more ▼]

Mitral insufficiency can have a dynamic character, correlating to an intermittent increase in the area of the regurgitant orifice and the regurgitant volume. Among the various stress methods, echocardiography during effort on a bicycle in the semisitting position appears to be the technique of choice for evaluating this dynamic character. Thanks to the improvement of the Doppler signal quality, quantification of the regurgitant volume variations can be obtained in a reproducible manner at rest and during effort by applying the "PISA" method. [less ▲]

Detailed reference viewed: 18 (0 ULg)
Full Text
Peer Reviewed
See detailElectrochemistry of lanthanum and uranium chlorides in organic media: Deposition of lanthanum and uranium
Martinot, Lucien; Lopes, L.; Marien, José ULg et al

in Journal of Radioanalytical & Nuclear Chemistry (2002), 253(3), 407-412

Molten salts have been widely used for the electrochemical preparation of lanthanum and uranium metals at high temperature. In this paper we demonstrate the feasibility of a similar process in ... [more ▼]

Molten salts have been widely used for the electrochemical preparation of lanthanum and uranium metals at high temperature. In this paper we demonstrate the feasibility of a similar process in dimethylformamide (DMF) and in the mixture gamma-butyrolactone/tetrahydrofuran (gamma-BL/THF) The best conditions for the preparation were deduced from preliminary transient electrochemistry experiments and from secondary ions mass spectrometry (SIMS) measurements involving SIMS mappings and SIMS depth profile analyses. [less ▲]

Detailed reference viewed: 39 (2 ULg)
Full Text
Peer Reviewed
See detailLong-term neurodevelopmental outcomes in school-aged children after neonatal arterial switch operation.
Hovels-Gurich, Hedwig H; SEGHAYE, Marie-Christine ULg; Schnitker, Ralph et al

in Journal of Thoracic and Cardiovascular Surgery (The) (2002), 124(3), 448-58

OBJECTIVE: Neurodevelopmental status of children between 8 and 14 years of age after neonatal arterial switch operation for transposition of the great arteries has not previously been systematically ... [more ▼]

OBJECTIVE: Neurodevelopmental status of children between 8 and 14 years of age after neonatal arterial switch operation for transposition of the great arteries has not previously been systematically evaluated. METHODS: Within a longitudinal study, 60 unselected children operated on as neonates with combined deep hypothermic circulatory arrest and low-flow cardiopulmonary bypass were reevaluated at the age of 7.9 to 14.3 years (mean +/- SD 10.5 +/- 1.6 years). Clinical neurologic status and standardized tests to assess gross motor function, intelligence, acquired abilities, language, and speech were carried out, and the results were related to preoperative, perioperative, and postoperative status, to management, and to neurodevelopmental status at a mean age of 5.4 years. RESULTS: Neurologic and speech impairments were evidently more frequent (27% and 40%, respectively) than in the general population. Intelligence and socioeconomic status were not different (P =.29 and P =.11), whereas motor function, acquired abilities, and language were reduced (P < or =.04 for each). Overall rate of developmental impairment in one or more domains was 55%, compared with 26% at age 5.4 years. Multivariable analysis showed that severe preoperative acidosis and hypoxia predicted reduced motor function (mean deficit 52.7 points, P <.001), whereas longer bypass duration predicted both neurologic (odds ratio per 10 minutes of bypass duration 1.8, P =.04) and speech (odds ratio per 10 minutes of bypass duration 1.9, P =.02) dysfunction, and perioperative and postoperative cardiocirculatory insufficiency predicted neurologic (odds ratio 6.5, P =.04) and motor (mean deficit 6.8 points, P =.03) dysfunction. CONCLUSIONS: The neonatal arterial switch operation with combined circulatory arrest and low-flow bypass is associated increasingly with age, with reduced neurodevelopmental outcome but not with cognitive dysfunction. In our experience, the risk of long-term neurodevelopmental impairment after neonatal corrective cardiac surgery is related to deleterious effects of the global perioperative management and to special adverse effects of prolonged bypass duration. Severe preoperative acidosis and hypoxia and postoperative hemodynamic instability must be considered as important additional risk factors. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Full Text
Peer Reviewed
See detailOntogenetic variations of thermal optimum for growth, and its implication on thermolabile sex determination in blue tilapia
Baras, E.; Mpo'n'tcha, A.; Driouch, H. et al

in Journal of Fish Biology (2002), 61(3), 645-660

Knowledge of how the optimum temperature for growth (Tdegrees(opt)) varies during ontogeny, and how close it is to the temperatures that induce Phenotypic masculinization is fundamental to the ... [more ▼]

Knowledge of how the optimum temperature for growth (Tdegrees(opt)) varies during ontogeny, and how close it is to the temperatures that induce Phenotypic masculinization is fundamental to the understanding of the evolution of thermolabile sex determinism (TSD) in fishes. In blue tilapia Oreochromis aureus, Tdegrees(opt) is 32.6degrees C at the start of exogenous feeding (10 mg fish) and it decreases by c 1degrees C each time that the fish bode mass increases by an order of magnitude. Temperatures <35degrees C are not sufficient to induce complete phenotypic masculinization. Based on a multiple-regression model (r(2)=0.938) plotting growth against body mass and water temperature. genotypically female tilapia living at high temperatures during the thermosensitive period (21-28 days) and being reversed into phenotypic males should incur an initial growth disadvantage over fish living at Tdegrees(opt) but not over those living at slightly colder temperatures (27-29degrees C). This initial disadvantage would be later compensated for by faster growth because of between-sex growth dimorphism to the detriment of phenotypic females. These arguments suggest that there is no definite pressure against the selection of TSD in blue tilapia and probable other Oreochromis spp. (C) 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 36 (8 ULg)
Full Text
Peer Reviewed
See detailIndexation et organisation de la connaissance. La régulation des décisions sur un forum de discussion
Lejeune, Christophe ULg

in Cahiers du Numérique (2002), 3(2), 129-144

This article presents the problems lived by a team of indexers organized in network that coordinates its activities by using discussion forum. The author aim to follow the coding problem solving that ... [more ▼]

This article presents the problems lived by a team of indexers organized in network that coordinates its activities by using discussion forum. The author aim to follow the coding problem solving that occurs in this project. The ongoing achievement of indexation raises up the question of the social, cognitive, political and quantitative representation and leads actors to compose associations’ networks (between sources of information, keywords, databases and indexers). The willing to collaborate and to communicate constraints them to coordinate and find agreement; discussions show the diverse and unstable forms of agreement processes. [less ▲]

Detailed reference viewed: 247 (38 ULg)
Full Text
Peer Reviewed
See detailCrystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis
Van Petegem, F.; Collins, T.; Meuwis, Marie-Alice ULg et al

in Acta Crystallographica Section D-Biological Crystallography (2002), 58(Part 9), 1494-1496

The 46 kDa xylanase from the Antarctic microorganism Pseudoalteromonas haloplanktis is an enzyme that efficiently catalyzes reactions at low temperatures. Here, the crystallization of both the native ... [more ▼]

The 46 kDa xylanase from the Antarctic microorganism Pseudoalteromonas haloplanktis is an enzyme that efficiently catalyzes reactions at low temperatures. Here, the crystallization of both the native protein and the SeMet-substituted enzyme and data collection from both crystals using synchrotron radiation are described. The native data showed that the crystals diffract to 1.3 Angstrom resolution and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.87, b = 90.51, c = 97.23 Angstrom. SAD data collected at the peak of the selenium absorption edge proved to be sufficient to determine the heavy-atom configuration and to obtain electron density of good quality. [less ▲]

Detailed reference viewed: 15 (5 ULg)
Full Text
See detailEffets de divers types de matières organiques en comparaison avec une fertilisation minérale sur les propriétés et la production d'un sol acide au Rwanda
Culot, Marc ULg; Mbonigaba Muhinda, Jean-Jacques; Bizimara, R.

in Etudes Rwandaises (2002), 5

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailThe impact of small physical obstacles on upstream movements of six species of fish - Synthesis of a 5-year telemetry study in the River Meuse basin
Ovidio, Michaël ULg; Philippart, Jean-Claude ULg

in Hydrobiologia (2002), 483(1-3), 55-69

In the course of the 'Meuse Salmon 2000' programme, most weirs and dams (3-8 m in height) in the regulated River Meuse have been progressively equipped with new fishways in order to restore the free ... [more ▼]

In the course of the 'Meuse Salmon 2000' programme, most weirs and dams (3-8 m in height) in the regulated River Meuse have been progressively equipped with new fishways in order to restore the free circulation of all amphibiotic fish species. Nevertheless, fish entering into major spawning tributaries are still confronted with various kinds of physical obstacles of which the overall impact on fish migration has never been investigated. In order to test their ability to negotiate physical obstacles, 128 individuals of fish ( Salmo trutta, Thymallus thymallus, Salmo salar, Chondrostoma nasus, Barbus barbus and Esox lucius) were captured several weeks before their spawning migrations and tagged with radio-transmitters. They were tracked from 30 to 466 days in the River Ourthe and six spawning tributaries over the period October 1995 to June 2001. All obstacles recorded in this study have been classified according to their type and main characteristics (i.e. slope, length and height). Results indicated that most fish migrate during or outside the spawning period and that some small obstacles are not as insignificant as initially thought and can significantly disrupt and/or obstruct their upstream movements. There is a need to harmonize interests in the sustainable conservation of fish populations and the development of small-scale hydropower generation and tourism. [less ▲]

Detailed reference viewed: 145 (26 ULg)
Full Text
Peer Reviewed
See detailClinical applications of assessment of airway inflammation using induced sputum
Pavord, I. D.; Sterk, P. J.; Hargreave, F. E. et al

in European Respiratory Journal (2002), 20(Suppl. 37), 40-43

Detailed reference viewed: 15 (2 ULg)
Full Text
Peer Reviewed
See detailMethod for estimation of low outer membrane permeability to beta-lactam antibiotics
Lakaye, Bernard ULg; Dubus, Alice ULg; Joris, Bernard ULg et al

in Antimicrobial Agents and Chemotherapy (2002), 46(9), 2901-2907

The outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux ... [more ▼]

The outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux systems. Up to now, the quantitative estimation of low outer membrane permeability by the method of Zimmermann and Rosselet was difficult because of the secreted and cell surface-associated beta-lactamases. The method presented here uses the acylation of a highly sensitive periplasmic penicillin-binding protein (PBP) (BlaR-CTD) to assess the rate of beta-lactam penetration into the periplasm. The method is dedicated to measurement of low permeability and is only valid when the diffusion rate through the outer membrane is rate limiting. Cytoplasmic membrane associated PBPs do not interfere since they are acylated after the very sensitive BlaR-CTD. This method was used to measure the permeability of beta-lactamase-deficient strains of Enterobacter cloacae and Enterobacter aerogenes to benzylpenicillin, ampicillin, carbenicillin, cefotaxime, aztreonam, and cephacetrile. Except for that of cephacetrile, the permeability coefficients were equal to or below 10(-7) cm/s. For cephacetrile, carbenicillin, and benzylpenicillin, the outer membrane of E. cloacae was 20 to 60 times less permeable than that of Escherichia coli, whereas for cefotaxime, aztreonam, and ampicillin it was, respectively, 400, 1,000, and 700 times less permeable. The permeability coefficient for aztreonam is the lowest ever measured (P = 3.2 X 10(-9) cm/s). Using these values, the MICs for a beta-lactamase-overproducing strain of E. cloacae were successfully predicted, demonstrating the validity of the method. [less ▲]

Detailed reference viewed: 40 (10 ULg)
Full Text
Peer Reviewed
See detailThe spatial epidemiology of cholera in an endemic area of Bangladesh
Ali, M.; Emch, M.; Donnay, Jean-Paul ULg et al

in Social Science & Medicine (2002), 55(6), 1015-1024

This paper defines high-risk areas of cholera based on environmental risk factors of the disease in an endemic area of Bangladesh. The risk factors include proximity to surface water, high population ... [more ▼]

This paper defines high-risk areas of cholera based on environmental risk factors of the disease in an endemic area of Bangladesh. The risk factors include proximity to surface water, high population density, and low educational status, which were identified in an earlier study by the authors. Cholera data were analyzed by spatially referenced extended household units for two time periods, 1983-1987 and 1992-1996. These periods were chosen because they had different dominant cholera agents. From 1983-1987 classical cholera was dominant and from 1992-1996 El Tor was dominant. By defining high-risk areas based on risk factors, this study builds a spatial risk model for cholera. The model is then evaluated based on the locations of observed cholera cases. The study also identifies the determinants of death due to cholera for the two different time periods dominated by the different cholera agents. The modeled risk areas that were based on the risk factors were found to correspond with actual distributions of cholera morbidity and mortality. The high-risk areas of the dominant cholera agents are relatively stable over time. However, from 1983-1987 El Tor cholera, which was not the dominant agent during that period, was not associated with high-risk areas, suggesting that the El Tor habitat may have changed over time. The case fatality rate for cholera was related to proximity to a diarrhea treatment hospital in the study area. (C) 2002 Published by Elsevier Science Ltd. [less ▲]

Detailed reference viewed: 113 (1 ULg)
Full Text
Peer Reviewed
See detailThe evolved early-type binary HDE 228766 revisited
Rauw, Grégor ULg; Crowther, P. A.; Eenens, P. R. J. et al

in Astronomy and Astrophysics (2002), 392(2), 563-574

We use an extensive set of spectroscopic observations to reinvestigate the properties of the massive binary HDE 228766. Conventional classification criteria suggest that HDE 228766 consists of an O7 ... [more ▼]

We use an extensive set of spectroscopic observations to reinvestigate the properties of the massive binary HDE 228766. Conventional classification criteria suggest that HDE 228766 consists of an O7 primary and an Of(+) secondary. However, several spectral features of the secondary, such as the simultaneous presence of N III, N IV and N V emissions, make it a rather unusual object. We find that the orbital motion of the secondary is probably best described by the radial velocities of the narrow N III emission lines. Our orbital solution yields m(1) sin(3) i = 31:7 and m(2) sin(3) i = 25:5 M. for the primary and secondary respectively. The He II lambda 4542 absorption in the secondary's spectrum appears considerably blue-shifted with respect to the narrow emission lines, indicating that the absorption is probably formed in the accelerating part of the secondary's wind. We use a tomographic technique to investigate the profile variability of the broader emission lines. In addition to a strong emission from the secondary, the Halpha line displays a weak emission feature that is probably associated with a wind interaction region located near the surface of the primary star. Finally, our analysis of the spectrum with a non-LTE code indicates that the secondary is an evolved object that exhibits some CNO processed material in its atmosphere and has a large mass loss rate. Assuming a distance of 3.5 kpc (which follows from adopting M-V,M-s = -6 for the secondary) we infer (M) over dot similar or equal to 10(5) M. yr(-1) for the secondary. Our results suggest that HDE 228766 could be in a post-Roche lobe overflow stage. The secondary may be classified as WN8ha and is currently in a transition stage between a "normal" Of staranda WNL-type Wolf-Rayet star. [less ▲]

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailNovel antiangiogenic effects of the bisphosphonate compound zoledronic acid
Wood, J.; Bonjean, K.; Ruetz, S. et al

in Journal of Pharmacology and Experimental Therapeutics (2002), 302(3), 1055-1061

Bisphosphonate drugs inhibit osteoclastic bone resorption and are widely used to treat skeletal complications in patients with tumor-induced osteolysis. We now show that zoledronic acid, a new generation ... [more ▼]

Bisphosphonate drugs inhibit osteoclastic bone resorption and are widely used to treat skeletal complications in patients with tumor-induced osteolysis. We now show that zoledronic acid, a new generation bisphosphonate with a heterocyclic imidazole substituent, is also a potent inhibitor of angiogenesis. In vitro, zoledronic acid inhibits proliferation of human endothelial cells stimulated with fetal calf serum, basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (IC50 values 4.1, 4.2, and 6.9 muM, respectively), and modulates endothelial cell adhesion and migration. In cultured aortic rings and in the chicken egg chorioallantoic membrane assay, zoledronic acid reduces vessel sprouting. When administered systemically to mice, zoledronic acid potently inhibits the angiogenesis induced by subcutaneous implants impregnated with bFGF [ED50, 3 mug/kg (7.5 nmol/kg) s.c.]. These findings indicate that zoledronic acid has marked antiangiogenic properties that could augment its efficacy in the treatment of malignant bone disease and extend its potential clinical use to other diseases with an angiogenic component. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
Peer Reviewed
See detailPharma clinics le medicament du mois. L'esomeprazole
Louis, Edouard ULg

in Revue Médicale de Liège (2002), 57(9), 610-2

Esomeprazole is the last PPI registered on the Belgian market. It is the stable s-isomer of omeprazole. It has a better pharmacokinetic profile than omeprazole (racemate), allowing also better clinical ... [more ▼]

Esomeprazole is the last PPI registered on the Belgian market. It is the stable s-isomer of omeprazole. It has a better pharmacokinetic profile than omeprazole (racemate), allowing also better clinical performances. Esomeprazole is the first PPI shown superior to omeprazole in acute and chronic treatment of gastro-esophageal reflux disease. Controlled trials with this drug have also allowed to define new cost-effective strategies, such as on demand treatment for endoscopy-negative gastro-esophageal reflux and one week treatment of Helicobacter pylori positive duodenal ulcer. [less ▲]

Detailed reference viewed: 76 (2 ULg)
Full Text
Peer Reviewed
See detailDiscrimination between Alzheimer dementia and controls by automated analysis of multicenter FDG PET
Herholz, K.; Salmon, Eric ULg; Perani, D. et al

in Neuroimage (2002), 17(1), 302-316

A new diagnostic indicator of FDG PET scan abnormality, based on age-adjusted t statistics and an automated voxel-based procedure, is presented and validated in a large data set comprising 110 normal ... [more ▼]

A new diagnostic indicator of FDG PET scan abnormality, based on age-adjusted t statistics and an automated voxel-based procedure, is presented and validated in a large data set comprising 110 normal controls and 395 patients with probable Alzheimer's disease (AD) that were studied in eight participating centers. The effect of differences in spatial resolution of PET scanners was minimized effectively by filtering and masking. In controls FDG uptake declined significantly with age in anterior cingulate and frontolateral perisylvian cortex. In patients with probable AD decline of FDG uptake in posterior cingulate, temporoparietal, and prefrontal association cortex was related to dementia severity. These effects were clearly distinct from age effects in controls, suggesting that the disease process of AD is not related to normal aging. Women with probable AD had significantly more frontal metabolic impairment than men. The new indicator of metabolic abnormality in AD-related regions provided 93% sensitivity and specificity for distinction of mild to moderate probable AD from normals, and 84% sensitivity at 93% specificity for detection of very mild probable AD (defined by Mini Mental Score 24 or better). All regions related to AD severity were already affected in very mild AD, suggesting that all vulnerable areas are affected to a similar degree already at disease onset. Ventromedial frontal cortex was also abnormal. In conclusion, automated analysis of multicenter FDG PET is feasible, provides insights into AD pathophysiology, and can be used potentially as a sensitive biomarker for early AD diagnosis. (C) 2002 Elsevier Science (USA). [less ▲]

Detailed reference viewed: 68 (3 ULg)
Full Text
Peer Reviewed
See detailLevels and profiles of PCDDs, PCDFs and cPCBs in Belgian breast milk. Estimation of infant intake
Focant, Jean-François ULg; Pirard, Catherine ULg; Thielen, Caroline ULg et al

in Chemosphere (2002), 48(8), 763-770

Congener-specific analyses of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and non-ortho (coplanar) polychlorinated biphenyls (cPCBs) were performed on 20 non-pooled ... [more ▼]

Congener-specific analyses of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and non-ortho (coplanar) polychlorinated biphenyls (cPCBs) were performed on 20 non-pooled breast milk samples collected in or close to an industrial area of Wallonia (Belgium). PCDD/F concentrations ranged between 16.0 and 52.1 pg TEQ/g fat, with a mean value of 29.4 pg TEQ/g fat. If coplanar PCBs (77, 126, 169) are included in TEQ calculations, levels ranged between 22.2 and 100.2 pg TEQ/g fat, with a mean value of 40.8 pg TEQ/g fat. It appears that 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, 2,3,4,7,8-PeCDF and PCB-126 account for more than 90% of the TEQ. Estimated PCDD/F dietary intake is 76 pg TEQ/kg body weight (bw)/day. This value is almost 20 times higher than the World Health Organization tolerable daily intake. A value of 103 pg TEQ/kg bw/day represents the intake of PCDDs, PCDFs and cPCBs (no mono-ortho PCBs included). (C) 2002 Published by Elsevier Science Ltd. [less ▲]

Detailed reference viewed: 47 (7 ULg)
Full Text
Peer Reviewed
See detailLe cinéma d’animation et ses thanatomorphoses (fragments sur le monstre, la charogne, le montage et l’animation)
Tomasovic, Dick ULg

in Cinémas : revue d'études cinématographiques / Cinémas: Journal of Film Studies (2002), 13(Automne), 143-164

The role and limits of editing in animation film are reconsidered here. It is no longer a matter of perceiving this practice as “simply” assembling shots, associating random elements, linking diverse ... [more ▼]

The role and limits of editing in animation film are reconsidered here. It is no longer a matter of perceiving this practice as “simply” assembling shots, associating random elements, linking diverse pieces and creating a monstrous body, but rather, it is presently regarded as a continual process of managing transitions, shifting between images, transforming inanimate objects, transmuting immobile elements and transfiguring inert beings. Thus, the animation film’s purpose is less phantasmal, to be a giver of life, as it is phantasmagoric, to be an animator of death. [less ▲]

Detailed reference viewed: 99 (10 ULg)
Full Text
Peer Reviewed
See detailStereotactic breast biopsy with an 8-gauge, directional, vacuum-assisted probe: initial experience
Lifrange, Eric ULg; Dondelinger, Robert ULg; Quatresooz, Pascale ULg et al

in European Radiology (2002), 12(9), 2180-2187

This study was prospectively conducted to assess the feasibility, safety and accuracy of an 8-G directional vacuum-assisted biopsy (DVAB) probe in the diagnostic management of nonpalpable breast lesions ... [more ▼]

This study was prospectively conducted to assess the feasibility, safety and accuracy of an 8-G directional vacuum-assisted biopsy (DVAB) probe in the diagnostic management of nonpalpable breast lesions (NPBL). Of 170 planned procedures which were indicated for investigation of BI-RADS category-3 to category-5 lesions, 153 were performed in 138 consecutive patients. The probe was targeted by the stereotactic unit of a prone table (United States Surgical Corporation, Norwalk, Conn.; and Lorad, Danbury, Conn.). Four to 18 (mean 8) core specimens were obtained for each lesion. In case of complete removal of the lesion, a localizing clip was deployed at the biopsy site. Adequate material for histopathologic examination was obtained in all cases (100%). Four of 138 (3%) patients experienced mild hematomas. We observed 15 of 39 failures (38%) to place the localizing clips. Thirteen of 153 (8%) procedures were inconclusive and required reintervention. Following DVAB, 42 of 138 (30%) patients underwent surgery. Subject to incomplete follow-up of the entire cohort, we observed no false-positive and one false-negative diagnosis. These preliminary results suggest that DVAB using an 8-G probe are feasible, safe and accurate. In our experience, clip placement was problematic. It is probable that increasing the dimensions of DVAB will only be relevant in a limited number of clinical situations, primarily the desire to obtain complete radiologic resections of the target abnormality. [less ▲]

Detailed reference viewed: 31 (6 ULg)
Full Text
Peer Reviewed
See detailLa fenetre implantatoire
Perrier D'Hauterive, Sophie ULg; Charlet, Jeanne de Chantal ULg; Goffin, Frédéric ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2002), 31(5), 440-55

BACKGROUND: Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this endometrium. Though implantation ... [more ▼]

BACKGROUND: Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this endometrium. Though implantation could occur in any human tissue, the endometrium is the only tissue where embryo implantation cannot occur except during a restricted period called the implantation window. During this window, the endometrium is highly receptive to the embryo. MATERIAL: and methods. We reviewed the literature concerning the different factors involved in improved endometrial receptivity and implantation. RESULTS: Maternal - embryo crosstalk is favored by the implantation window. Endometrial receptivity results from the acquisition of ligands or receptors facilitating apposition, then adhesion of the embryo, or from the loss of components preventing it. The molecular basis of the implantation window remains to be defined. CONCLUSION: Despite progress in assisted reproduction technologies, the lack of control of implantation remains a major obstacle to successful pregnancy. It is of prime importance to determine the characteristic features of a receptive endometrium and, among the many markers proposed by in vitro studies, to analyze in humans those demonstrated by knock-out experiments to play a crucial role in mice. [less ▲]

Detailed reference viewed: 109 (3 ULg)
Full Text
Peer Reviewed
See detailMultifocal serous cystadenoma of the pancreas synchronous with ampullary adenocarcinoma
Quatresooz, Pascale ULg; Honore, Pierre ULg; Vivario, M. et al

in Annales de Pathologie (2002), 22(4), 317-320

Pancreatic cysts are common, but cystic tumors are uncommon. We report a rare case of serous cystadenoma of the pancreas synchronous with ampullary adenocarcinoma which supports a common etiopathogeny of ... [more ▼]

Pancreatic cysts are common, but cystic tumors are uncommon. We report a rare case of serous cystadenoma of the pancreas synchronous with ampullary adenocarcinoma which supports a common etiopathogeny of these tumors. We discuss the differential diagnosis with mucinous cystadenoma which is potentially malignant and recall the microscopic and radiologic features. [less ▲]

Detailed reference viewed: 28 (0 ULg)
Full Text
Peer Reviewed
See detailSystematic regularization of linear inverse solutions of the EEG source localization problem
Phillips, Christophe ULg; Rugg, Michael D; Friston, Karl J

in NeuroImage (2002), 17(1), 287-301

Distributed linear solutions of the EEG source localization problem are used routinely. Here we describe an approach based on the weighted minimum norm method that imposes constraints using anatomical and ... [more ▼]

Distributed linear solutions of the EEG source localization problem are used routinely. Here we describe an approach based on the weighted minimum norm method that imposes constraints using anatomical and physiological information derived from other imaging modalities to regularize the solution. In this approach the hyperparameters controlling the degree of regularization are estimated using restricted maximum likelihood (ReML). EEG data are always contaminated by noise, e.g., exogenous noise and background brain activity. The conditional expectation of the source distribution, given the data, is attained by carefully balancing the minimization of the residuals induced by noise and the improbability of the estimates as determined by their priors. This balance is specified by hyperparameters that control the relative importance of fitting and conforming to prior constraints. Here we introduce a systematic approach to this regularization problem, in the context of a linear observation model we have described previously. In this model, basis functions are extracted to reduce the solution space a priori in the spatial and temporal domains. The basis sets are motivated by knowledge of the evoked EEG response and information theory. In this paper we focus on an iterative "expectation-maximization" procedure to jointly estimate the conditional expectation of the source distribution and the ReML hyperparameters on which this solution rests. We used simulated data mixed with real EEG noise to explore the behavior of the approach with various source locations, priors, and noise levels. The results enabled us to conclude: M Solutions in the space of informed basis functions have a high face and construct validity, in relation to conventional analyses. (ii) The hyperparameters controlling the degree of regularization vary largely with source geometry and noise. The second conclusion speaks to the usefulness of using adaptative ReML hyperparameter estimates. (C) 2002 Elsevier Science (USA). [less ▲]

Detailed reference viewed: 32 (6 ULg)
Full Text
Peer Reviewed
See detailTraining early Alzheimer patients to use a mobile phone
Lekeu, Françoise ULg; Wojtasik, Vinciane ULg; Van der Linden, Martial ULg et al

in Acta Neurologica Belgica (2002), 102(3), 114-121

The mobile phone may be useful to keep in contact with spatially disoriented and memory impaired patients. In keeping with this idea, this study describes the training program developed to teach two ... [more ▼]

The mobile phone may be useful to keep in contact with spatially disoriented and memory impaired patients. In keeping with this idea, this study describes the training program developed to teach two patients with mild Alzheimer's disease (CI and ML) how to use their own mobile phone. Each training session was divided into two parts. In the first part, the spaced-retrieval technique was used to promote the consultation of a card pasted on the back of the phone. The card detailed each stage of phone utilization and which keys had to be pressed to call somebody. In the second part, the patients received repetitive exercises of calling based upon the errorless learning principle. At the end of three-months rehabilitation, the results showed different learning patterns for the patients. ML needed more spaced-retrieval sessions to spontaneously consult the card and to correctly use the phone, compared to CI However, by the repetition of calling exercises, both patients showed a decrease of instruction card consultation, whereas they were still able to correctly call somebody. This learning ability is hypothesized to be a consequence of a relatively preserved procedural memory in both patients. In conclusion, this study highlights the effectiveness of combined specific learning techniques for improving AD patient's autonomy in daily life activities. [less ▲]

Detailed reference viewed: 48 (4 ULg)
Full Text
Peer Reviewed
See detail5-HT2A receptor antagonism potentiates haloperidol-induced dopamine release in rat medial prefrontal cortex and inhibits that in the nucleus accumbens in a dose-dependent manner
Liégeois, Jean-François ULg; Ichikawa, J.; Meltzer, H. Y.

in Brain Research (2002), 947(2), 157-165

Combined serotonin (5-HT)(2A) and dopamine (DA) D-2 blockade has been shown to contribute to the ability of atypical antipsychotic drugs (APDs) to increase DA release in rat medial prefrontal cortex (mPFC ... [more ▼]

Combined serotonin (5-HT)(2A) and dopamine (DA) D-2 blockade has been shown to contribute to the ability of atypical antipsychotic drugs (APDs) to increase DA release in rat medial prefrontal cortex (mPFC). We provide additional support for this hypothesis by examining the effect of the selective 5-HT2A antagonist M100907 plus haloperidol, a potent D, antagonist APD, on DA release in the mPFC and nucleus accumbens (NAC). Haloperidol (0.01-1.0 mg/kg) produced an inverted U-shaped increase in DA release in the mPFC, with a significant increase only at 0.1 mg/kg. Haloperidol (0.1 and 1.0 mg/kg) significantly increased DA release in the NAC. M100907 (0.1 mg/kg) by itself had no effect on DA release in either region. This dose of M100907 potentiated the ability of low (0.01-0.1 mg/kg), but not high dose (0.3-1.0 mg/kg) haloperidol to increase mPFC DA release, whereas it abolished the effect of both 0.1 and 1.0 mg/ka haloperidol on NAC DA release. These results suggest that the relatively higher ratio of 5-HT2A to D-2 antagonism may contribute to the potentiation of haloperidol-induced mPFC DA release, whereas 5-HT2A antagonism can diminish haloperidol-induced NAC DA release, even when combined with extensive D, antagonism, which may not be synergistic with 5-HT2A antagonism in the mPFC. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 47 (3 ULg)
Full Text
Peer Reviewed
See detailTraitement moléculaire du cancer: le STI571, un inhibiteur des tyrosines kinases
Humblet-Baron, Stéphanie ULg; Baron, Frédéric ULg; Pirson, Laurence ULg et al

in Revue Médicale Suisse (2002), 60(598), 1504-1508

Detailed reference viewed: 120 (20 ULg)
Full Text
Peer Reviewed
See detailAAV2 vectors mediate efficient and sustained transduction of rat embryonic ventral mesencephalon
Lehtonen, E.; Bonnaud, F.; Melas, C. et al

in Neuroreport (2002), 13(12), 1503-1507

The success of transplantation of human embryonic mesencephalic tissue to treat parkinsonian patients is limited by the poor survival of the transplant. We show that an AAV2 vector mediates efficient ... [more ▼]

The success of transplantation of human embryonic mesencephalic tissue to treat parkinsonian patients is limited by the poor survival of the transplant. We show that an AAV2 vector mediates efficient expression of the egfp reporter gene in organotypic cultures of freshly explanted solid fragments of rat embryonic ventral mesencephalon (VM). We observed early and sustained transgene expression (4 days to greater than or equal to 6 weeks). Furthermore, rAAV-infected rat embryonic VM transplanted in the adult striatum continued to express EGFP for greater than or equal to 3 months. More than 95% of the transduced cells were neurons. Dopaminergic neurons were transduced at low frequency at earlier time points. This method of gene delivery could prove useful to achieve local, continuous secretion of neurotrophic factors at physiologically relevant doses to treat Parkinson's disease. [less ▲]

Detailed reference viewed: 19 (0 ULg)
Full Text
Peer Reviewed
See detailElectrical investigations of granular arches
Dorbolo, Stéphane ULg; Vandewalle, Nicolas ULg

in Physica A: Statistical Mechanics and its Applications (2002), 311(3-4), 307-312

Compaction dynamics and granular arches have been studied using the electrical resistance of a packing. An exponential decay of the resistance is found whatever the electrical current is. A fluctuation ... [more ▼]

Compaction dynamics and granular arches have been studied using the electrical resistance of a packing. An exponential decay of the resistance is found whatever the electrical current is. A fluctuation regime is then encountered. A characteristic relaxation time has been pointed out and is linked to the arch formation in the system. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 15 (5 ULg)
Full Text
Peer Reviewed
See detailOxytocin synthesis and oxytocin receptor expression by cell lines of human small cell carcinoma of the lung stimulate tumor growth through autocrine/paracrine signaling
Pequeux, Christel ULg; Breton, Christophe; Hendrick, Jean-Claude et al

in Cancer Research (2002), 62(16), 4623-4629

The objective of the present work was to investigate the existence of an oxytocin (OT)-mediated autocrine/paracrine signaling upon small cell carcinoma of the lung (SCCL) cell growth. In that view, OT ... [more ▼]

The objective of the present work was to investigate the existence of an oxytocin (OT)-mediated autocrine/paracrine signaling upon small cell carcinoma of the lung (SCCL) cell growth. In that view, OT receptor (OTR) expression, concomitant with OT synthesis and secretion, was evidenced on three different SCCL cell lines (DMS79, H146, and H345) and related to the vasopressin (VP) system. Specific OT, VP, OTR, Via VP receptor (V1aR), and V1b/V3 VP receptor (V1bR/V3R) transcripts were identified by reverse transcription-.PCR in all cell lines studied. Binding of I-125-(d(CH2)(5)(1),Tyr(Me)(2), Thr(4), Orn(3),Tyr(9)-NH2)-vasotocin (OVTA) was observed on all SCCL cell lines, with a K-d (dissociation constant) ranging from 0.025-0.089 nm, depending; on the cell line and the analytical method. Selectivity of I-125-OVTA binding was confirmed by displacement curves obtained with various OTR and VP receptor agonists and antagonists (OT, OVTA, L-371,257, VP, F180). Immunocytochemistry identified cellular OT and VP, and peptide secretion was measured in supernatants of SCCL cultures. [H-3]Thymidine incorporations, applied on H345 cells, demonstrated a dose-dependent mitogenic effect of exogenous OT (1 and 100 nM) that was abolished by the OTR antagonist OVTA. A decrease of proliferation was also observed with OVTA alone, showing a functional mitogenic effect of tumor-derived OT. Taken together, these observations demonstrate the existence of a functional OT-mediated autocrine/paracrine signaling actively implicated in growth and development of SCCL tumors. Furthermore, these findings point to the potential of OT antagonists for development as therapeutic agents for the treatment of SCCL. [less ▲]

Detailed reference viewed: 55 (7 ULg)
Full Text
Peer Reviewed
See detailpH Dependence of the morphology of aqueous micelles formed by polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide) copolymers
Gohy, Jean-François; Willet, Nicolas ULg; Varshney, Sunil K et al

in e-Polymers (2002), (35), 1-10

The morphology of micelles formed by two polystyrene-block-poly(2-vinylpyridine)- block-poly(ethylene oxide) (PS-b-P2VP-b-PEO) copolymers was studied in water by dynamic light scattering and transmission ... [more ▼]

The morphology of micelles formed by two polystyrene-block-poly(2-vinylpyridine)- block-poly(ethylene oxide) (PS-b-P2VP-b-PEO) copolymers was studied in water by dynamic light scattering and transmission electron microscopy. Spherical micelles were observed that consist of a PS core, a P2VP shell and a PEO corona. The characteristic size of core, shell and corona depends on the copolymer composition. An important increase in micellar size occurs at pH < 5 as a result of P2VP block protonation. The reversibility of this pH effect depends on copolymer composition, too. The conformation of the PEO block plays an important role in this pH driven morphological transition. [less ▲]

Detailed reference viewed: 83 (3 ULg)
Full Text
Peer Reviewed
See detailHuman breast adenocarcinoma cell lines promote angiogenesis by providing cells with uPA-PAI-1 and by enhancing their expression
Bajou, Khalid ULg; Lewalle, J. M.; Martinez, C. R. et al

in International Journal of Cancer = Journal International du Cancer (2002), 100(5), 501-506

During angiogenesis, endothelial cells use uPA and PAI-I to migrate and degrade the basement membrane surrounding capillary blood vessels. Invasive tumor cells produce a large amount of uPA that could ... [more ▼]

During angiogenesis, endothelial cells use uPA and PAI-I to migrate and degrade the basement membrane surrounding capillary blood vessels. Invasive tumor cells produce a large amount of uPA that could bind uPAR present at the endothelial cell surface to facilitate their invasion. To verify this hypothesis, endothelial cells were incubated with conditioned medium (CM) from two breast cancer cell lines (MCF7 and MDA MB 231 cells). Within a short incubation period (30 min) with both CM, an increase of uPA, PAW and uPA-PAI-I complex was detected in endothelial cell layer as assessed by casein zymography, ELISA and uPA immunostaining. The extent of this enhancement was related to the levels of uPA secreted by tumor cells (high in MDA MB 231 cells and low in MCF7 cells). After 2 hr of incubation, the CM from both tumor cells upregulated uPA and PAI-I mRNA levels in endothelial cells in a time-dependent manner. The uPA increase in the cell layer could not be attributable to an increase of uPAR level. Only the CM from highly invasive MDA MB 231 cells increased the angiogenic morphotype of endothelial cells assessed in a collagen gel. A single addition of amino-terminal fragment of uPA (ATF) was able to abolish the angiogenic effect induced by MDA MB 231 cell CM. Our data demonstrate that the interactions occurring between breast tumor cells and endothelial cells can modulate tumor angiogenesis at least by two mechanisms: an increase of uPA and PAI-I cell surface-binding and of their expression by endothelial cells. (C) 2002 Wiley-Liss, Inc. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Full Text
Peer Reviewed
See detailNovel unsaturated epsilon-caprolactone polymerizable by ring-opening and ring-opening metathesis mechanisms
Lou, Xudong; Detrembleur, Christophe ULg; Lecomte, Philippe ULg et al

in e-Polymers (2002), (34), 1-12

Ring-opening polymerization (ROP) and ring-opening metathesis polymerization (ROMP) of an unsaturated epsilon-caprolactone, 6,7-dihydro-2(3H)-oxepinone (DHO2), are alternative routes to produce ... [more ▼]

Ring-opening polymerization (ROP) and ring-opening metathesis polymerization (ROMP) of an unsaturated epsilon-caprolactone, 6,7-dihydro-2(3H)-oxepinone (DHO2), are alternative routes to produce unsaturated aliphatic polyesters with the same molecular structure. Polymerization of DHO2 initiated by Al isopropoxide in toluene at room temperature or at 0°C proceeds by a coordination-insertion mechanism, although intramolecular transesterification takes place beyond complete monomer conversion. The molecular weight distribution is narrow as long as monomer conversion does not exceed 90%. Ring-opening metathesis polymerization of DHO2 initiated by Schrock's Mo-based catalyst, 1, at 60°C allows higher molecular weight unsaturated polyester to be prepared, even though an intramolecular side reaction also operates. The structure of poly(DHO2) synthesized by ROP and ROMP is the same, as confirmed by 1H, 13C NMR, and FT-IR spectra. Copolymers of DHO2 with norbornene, cis-cyclooctene, and 1,5-cyclooctadiene have been successfully prepared. [less ▲]

Detailed reference viewed: 104 (6 ULg)