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See detailIdentification of coiled body-like structures in meristematic cells of Pisum sativum cotyledonary buds.
Jennane, A.; Thiry, Marc ULg; Goessens, Guy ULg

in Chromosoma (1999), 108(2), 132-42

This study focused on two types of nuclear bodies visible in plant cells that were previously thought to be similar to the coiled bodies (CBs) of animal cells: the nucleolus-associated body (NAB) and ... [more ▼]

This study focused on two types of nuclear bodies visible in plant cells that were previously thought to be similar to the coiled bodies (CBs) of animal cells: the nucleolus-associated body (NAB) and dense body (DB). We show that both NABs and DBs share common features with animal CBs: they consist of ribonucleoproteins, are silver-stainable, and lack DNA. Immunoelectron microscopy shows that only the NABs are rich in snRNAs and fibrillarin, two markers characteristic of animal CBs. This suggests that NABs rather than DBs are the plant counterparts of the CBs of animal cells. These structures appear most frequently in cells blocked in G0-1, their frequency gradually declining with resumption of the cell cycle and nucleolar activity. During this reactivation period, NABs are released from the nucleolus to the nucleoplasm, suggesting that they may act as nuclear transport or sorting structures. [less ▲]

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See detailSocioeconomic and psychosocial conditions of parents and cardiovascular risk factors in their children: the Belgian Luxembourg Child Study III.
Guillaume, Michèle ULg; Lapidus, L.; Lambert, A. et al

in Acta Paediatrica (1999), 88(8), 866-73

Socioeconomic and psychosocial handicaps are often associated with disease. There is a large body of information on adults on such relationships, but data are sparse on children. In a cohort of 1028 boys ... [more ▼]

Socioeconomic and psychosocial handicaps are often associated with disease. There is a large body of information on adults on such relationships, but data are sparse on children. In a cohort of 1028 boys and girls, selected at random from school classes in Province de Luxembourg, a mainly rural area of Belgium, these problems were analysed in age strata of 6-8, 8-10, 10-12 years. Participation rate was 71%. Information was collected from questionnaires,. Anthropometric variables, blood pressure and glucose as well as cholesterol, triglycerides and insulin were measured in the children and related to the psychosocial and socioeconomic factors, organized into rural, psychosocial, socioeconomic and alcohol/smoking clusters of observations. Rural: Duration of living in the area of the child and parents correlated with diastolic blood pressure, particularly in boys (p < 0.01). Psychosocial: Housewives (p = 0.002) and their children (p = 0.002) had higher body mass indexes (BMI) than other mothers and their children. Sons of housewives also had higher blood pressure (systolic, p = 0.0007, diastolic, p = 0.007). Socioeconomic: Socioeconomic factors of parents (profession, unemployment) played relatively minor roles. Alcohol/smoking: Alcohol consumption was related to skinfold thickness in boys (p = 0.022), but not in girls. Girls, but not boys, with smoking parents had higher BMI (p=0.014). Multiple regression analyses suggested that psychosocial factors, such as housewives as mothers of large families, may be important for associations with cardiovascular risk factors in their children. There were apparent differences in the findings between girls and boys, suggesting that boys are more vulnerable to the impacts of the factors analysed. [less ▲]

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See detailDefining obesity in childhood: current practice.
Guillaume, Michèle ULg

in American Journal of Clinical Nutrition (1999), 70(1), 126-30

A survey of information from 26 countries was performed to examine the methods, cutoff points, and reference materials used to define obesity in childhood and adolescence. The body mass index (in kg/m2 ... [more ▼]

A survey of information from 26 countries was performed to examine the methods, cutoff points, and reference materials used to define obesity in childhood and adolescence. The body mass index (in kg/m2) was used frequently, as well as several other methods. Reference materials used were often based on national surveys, although reference data from other countries were sometimes used. The data presented was often insufficient to judge the representativeness of the reference material. Cutoff points varied considerably. Available data allow neither a meaningful international estimation of the prevalence of obesity nor international comparisons. Although associated with considerable problems, this situation can be improved with an international consensus which, by necessity, will be riddled with uncertainties and compromises. [less ▲]

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See detailComment on "physical reality of light-induced atomic states"
Schlagheck, Peter ULg; Hornberger, K.; Buchleitner, A.

in Physical Review Letters (1999), 82(3), 664-664

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See detailSpreadsheet based teaching aids in chemical engineering education
Heyen, Georges ULg; Kalitventzeff, Boris ULg

in Computers & Chemical Engineering (1999), 23(Suppl. S), 629-632

Modern spreadsheet programs offer a wide range of calculation, charting and dialog options, allowing the development of effective teaching aids. Examples of worksheet illustrating numerical analysis ... [more ▼]

Modern spreadsheet programs offer a wide range of calculation, charting and dialog options, allowing the development of effective teaching aids. Examples of worksheet illustrating numerical analysis, thermodynamics and unit operations are shown. All offer complete interaction between the problem statement and the presentation of results. [less ▲]

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See detailModification of the Thermoresistance to Spray-Drying of a Cold-Adapted Subtilisin by Genetic Engineering
Bare, G.; Diakiese, A.; Zgoulli, S. et al

in Applied Biochemistry and Biotechnology (1999), 77-79(Spring), 857-65

The thermoresistance of a cold-adapted subtilisin dried by spray-drying was studied. Proteolytic activity of this enzyme was measured before and after spray-drying. Without chemical additives, spray ... [more ▼]

The thermoresistance of a cold-adapted subtilisin dried by spray-drying was studied. Proteolytic activity of this enzyme was measured before and after spray-drying. Without chemical additives, spray-drying yields ranged from 2-13%. The use of arabic gum and lactose in the composition of the enzyme solutions allowed the strengthening of the enzyme structures and increased water mobility in the product. Increase of water mobility led to a shorter residence time of the product in the spray-drier and a net yield increase was obtained (yield higher than 50%). The effect of two selective mutations on the thermoresistance to spray-drying of the cold-adapted subtilisin was also investigated. Mutation T85D (introduction of an additional link with an ion Ca2+ necessary for enzyme activity, by substitution of Asp for Thr 85) had no effect on the thermoresistance of the subtilisin to spray-drying. Mutation H121W (introduction of an additional aromatic link by substitution of Trp for His 121) reduced the drying yield from 66% (not modified subtilisin) to 52%. This higher thermosensitivity could be explained by an increase of the hygroscopic character of the modified subtilisin (mutation H121W). [less ▲]

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See detailModelling the butterfat crystallisation process
Heyen, Georges ULg; Alleman, Xavier; Kalitventzeff, Boris ULg et al

in Computers & Chemical Engineering (1999), 23(Suppl. S), 819-822

Milk fat exhibits seasonal variations in composition and properties which are undesirable for many subsequent applications. Thus techniques are sought to process dairy products in order to achieve a ... [more ▼]

Milk fat exhibits seasonal variations in composition and properties which are undesirable for many subsequent applications. Thus techniques are sought to process dairy products in order to achieve a consistent quality. A brief introduction to milk fat presents its most important particularities, especially composition, seasonal variations, solid fat content, crystalisation and polymorphism. The differential scanning calorimetry (DSC) analysis allows to estimate the solid fat content. Different methods have been developed to estimate more and more precisely the solid fat content which is certainly an important parameter in the description of the textural properties of butterfat. The industrial crystallisation process is modelled on the basis of pilot plant data. That model allows to approach the particular temperature profile in the scraped surface heat exchangers and to give a first explanation of the involved phenomena. [less ▲]

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See detailDesigned combination of chiral selectors for adjustment of enantioseparation selectivity in capillary electrophoresis.
Fillet, Marianne ULg; Chankvetadze, Bezhan; Crommen, Jacques ULg et al

in Electrophoresis (1999), 20(13), 2691-7

In this study an attempt has been made to explain the reasons for changing the enantioseparation selectivity in some dual cyclodextrin (CD) systems compared to the use of single chiral selectors in ... [more ▼]

In this study an attempt has been made to explain the reasons for changing the enantioseparation selectivity in some dual cyclodextrin (CD) systems compared to the use of single chiral selectors in capillary electrophoresis (CE). An explanation for selectivity changes is proposed based on the effect of the chiral selector on the mobility of the analyte. In order to support the proposed mechanism, several dual systems were designed on the basis of the known recognition pattern of enantiomers for individual CDs. In most cases the separation selectivity could be adjusted in a designed way. There was no experimental evidence for simultaneous binding of a given chiral analyte with both chiral selectors or of chiral recognition of an analyte complex with one CD by another CD. [less ▲]

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See detailElimination of adsorption effects in the analysis of water-soluble vitamins in pharmaceutical formulations by capillary electrophoresis.
Fotsing, Lucas; Fillet, Marianne ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (1999), 853(1-2), 391-401

A tendency to an increase in migration times was observed when different water-soluble vitamins were analysed repeatedly in pharmaceutical preparations by capillary electrophoresis. In order to better ... [more ▼]

A tendency to an increase in migration times was observed when different water-soluble vitamins were analysed repeatedly in pharmaceutical preparations by capillary electrophoresis. In order to better understand the origin of this effect, the influence of the vitamins and the excipients, such as cellulose derivatives, was investigated. These studies indicated that the increase in analyte migration times was most probably due to the adsorption of different kinds of constituents to the capillary wall. Different rinsing procedures were tested in order to eliminate these unfavourable effects. A rinse of the capillary with a 25 mM sodium dodecyl sulfate (SDS) solution in the running buffer between successive runs was found to be particularly effective when the analysis was performed by free solution capillary zone electrophoresis (CZE). When the vitamins were determined by micellar electrokinetic chromatography (MEKC) using SDS as surfactant, a short capillary rinse with the running buffer was sufficient to obtain reproducible migration times. The CZE and MEKC methods developed were validated and compared. Both methods could be applied to the determination of water-soluble vitamins in different multivitamin formulations. [less ▲]

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See detailAtteintes physiopathologiques liees a la circulation extracorporelle. Pathogenies et modes de prevention
Defraigne, Jean-Olivier ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(10-12), 381-91392-5

During heart surgery, several humoral cascades (coagulation, complement, kallicrein-kinin, cytokines, fibrinolysis) and several cell systems (platelets, neutrophils, endothelial cells, ...) are activated ... [more ▼]

During heart surgery, several humoral cascades (coagulation, complement, kallicrein-kinin, cytokines, fibrinolysis) and several cell systems (platelets, neutrophils, endothelial cells, ...) are activated. Numerous contributing factors have been reported: blood contact with foreign surfaces of the extracorporeal circuits, blood-air interface, lung and myocardial ischemia-reperfusion after unclamping, hypothermia, shear stresses, ... A post-perfusion syndrome may develop which include miscellaneous symptoms: coagulation disturbances and bleeding, neurological alterations, inflammatory syndrome, and, in extreme cases, multisystemic organ failure. Even if the present mortality of cardiac surgery is low, several approaches have been proposed to reduce such activations. They are based on changing in the circuit design, or in the composition of the luminal surfaces of the tubing and oxygenator, on improvement of the operative technique, and on modifications of the perfusion technique. Pharmacological agents are also used (anti-inflammatory drugs, corticoids, serine proteases inhibitor (aprotinin, ...). Nevertheless, the development of more biocompatible surfaces seems a promising goal. [less ▲]

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See detailLes biotechnologies vegetales appropriees dans le contexte du dialogue Nord-Sud.
Lepoivre, Philippe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(1),

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See detailDendrimer-like star block and amphiphilic copolymers by combination of ring opening and atom transfer radical polymerization
Hedrick, James L.; Trollsas, Mikael; Hawker, Craig Jon et al

in Macromolecules (1998), 31(25), 8691-8705

A new type of macromolecular architecture, denoted as dendrimer-like star block copolymers, is reported. These block copolymers are described by a radial geometry where the different generations or layers ... [more ▼]

A new type of macromolecular architecture, denoted as dendrimer-like star block copolymers, is reported. These block copolymers are described by a radial geometry where the different generations or layers are comprised of high molecular weight polymer emanating from a central core. A hexahydroxyl functional core was used as an initiator for the “living” ring opening polymerization (ROP) of ε-caprolactone producing a hydroxyl terminated six arm star polymer with controlled molecular weight and narrow polydispersities (PD < 1.1). Capping these chain ends with dendrons containing activated bromide moieties produced “macro-initiators” for atom transfer radical polymerization (ATRP). Methyl methacrylate was polymerized from these “macro-initiators” in the presence of an organometallic promoter to produce the requisite dendrimer-like star polymers. High molecular weight was obtained with low polydispersities (<1.2). Alternatively, amphiphilic character could be introduced by designing the different layers or generations to be either hydrophobic or hydrophilic. For example, methyl methacrylate (MMA) with either hydroxyethyl methacrylate (HEMA) or methacrylate functional ethylene oxide macromonomers (EO) were polymerized from these “macro-initiators” to provide a hydrophilic outer layer. The use of macromolecular building blocks allows rapid attainment of high polymer in a limited number of steps with purification between transformation requiring only polymer precipitation. [less ▲]

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See detailLa résistance bactérienne aux antibiotiques, un modèle exemplaire d'évolution moléculaire dirigée
Ghuysen, Jean-Marie ULg

in Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie (1998), 60(4), 287-294

The simplest conceivable event that can occur at the gene level can result in the development of efficacious resistance tot antibiotics. The bacterial world behaves as an enormous organism whose cells can ... [more ▼]

The simplest conceivable event that can occur at the gene level can result in the development of efficacious resistance tot antibiotics. The bacterial world behaves as an enormous organism whose cells can exchange their genes very easily. Accordingly, oppotunities for the exhcange of genetic material in nature are probably unlimited. This knowledge cannot be ignored. It leads to the important conclusion that the antibiotics are societal drugs. A resistance gene which has appeared somewhere in the world can travel far and fast. [less ▲]

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See detailTransport of arginine and ornithine into isolated mitochondria of Saccharomyces cerevisiae.
Soetens, Oriane; Crabeel, Marjolaine; Elmoualij, Benaïssa ULg et al

in European Journal of Biochemistry (1998), 258(2), 702-9

In this work we have characterised the transport of L-arginine and L-ornithine into mitochondria isolated from a wild-type Saccharomyces cerevisiae strain and an isogenic arg11 knock-out mutant. The Arg11 ... [more ▼]

In this work we have characterised the transport of L-arginine and L-ornithine into mitochondria isolated from a wild-type Saccharomyces cerevisiae strain and an isogenic arg11 knock-out mutant. The Arg11 protein (Arg11p) is a mitochondrial carrier required for arginine biosynthesis [Crabeel, M., Soetens, O., De Rijcke, M., Pratiwi, R. & Pankiewicz, R. (1996) J. Biol. Chem. 271, 25011-25019]. Reconstitution experiments have confirmed that it is an L-ornithine carrier also transporting L-arginine and L-lysine by order of decreasing affinity, but not L-histidine [Palmieri, L., De Marco, V., Iacobazzi, V., Palmieri, F., Runswick, M. & Walker, J. (1997) FEBS Lett. 410, 447-451]. Evidence is presented here that the mitochondrial inner membrane contains an L-arginine and L-ornithine transporting system distinct from Arg11p, in keeping with the arginine leaky phenotype of arg11 knock-out mutants. The newly characterised carrier, which we propose to name Bac1p (basic amino acid carrier), behaves as an antiporter catalysing the electroneutral exchange of the basic amino acids L-arginine, L-lysine, L-ornithine and L-histidine and displays the highest affinity for L-arginine (Km of 30 microM). L-Arginine uptake has a pH optimum in the range of 7.5-9 and is inhibited by several sulphydryl reagents, by pyridoxal 5'-phosphate and by cations [less ▲]

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See detailObservations of Coronal Structures Above an Active Region by EIT and Implications for Coronal Energy Deposition
Neupert, W. M.; Newmark, J.; Delaboudinière, J.-P. et al

in Solar Physics (1998), 183

Solar EUV images recorded by the EUV Imaging Telescope (EIT) on SOHO have been used to evaluate temperature and density as a function of position in two largescale features in the corona observed in the ... [more ▼]

Solar EUV images recorded by the EUV Imaging Telescope (EIT) on SOHO have been used to evaluate temperature and density as a function of position in two largescale features in the corona observed in the temperature range of 1.0-2.0MK. Such observations permit estimates of longitudinal temperature gradients (if present) in the corona and, consequently, estimates of thermal conduction and radiative losses as a function of position in the features. We examine two relatively cool features as recorded in EIT's Feix/x (171Å) and Fexii (195Å) bands in a decaying active region. The first is a long-lived loop-like feature with one leg, ending in the active region, much more prominent than one or more distant footpoints assumed to be rooted in regions of weakly enhanced field. The other is a near-radial feature, observed at the West limb, which may be either the base of a very high loop or the base of a helmet streamer. We evaluate energy requirements to support a steady-state energy balance in these features and find in both instances that downward thermal conductive losses (at heights above the transition region) are inadequate to support local radiative losses, which are the predominant loss mechanism. The requirement that a coronal energy deposition rate proportional to the square of the ambient electron density (or pressure) is present in these cool coronal features provides an additional constraint on coronal heating mechanisms. [less ▲]

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See detailPolarization properties of a sample of broad absorption line and gravitationally lensed quasars
Hutsemekers, Damien ULg; Lamy, H.; Remy, M.

in Astronomy and Astrophysics (1998), 340

New broad-band linear polarization measurements have been obtained for a sample of 42 optically selected QSOs including 29 broad absorption line (BAL) QSOs. The polarization properties of different sub ... [more ▼]

New broad-band linear polarization measurements have been obtained for a sample of 42 optically selected QSOs including 29 broad absorption line (BAL) QSOs. The polarization properties of different sub-classes have been compared, and possible correlations with various spectral indices searched for. The main results of our study are: (1) Nearly all highly polarized QSOs of our sample belong to the sub-class of BAL QSOs with low-ionization absorption features (LIBAL QSOs). (2) The range of polarization is significantly larger for LIBAL QSOs than for high-ionization (HI) BAL QSOs and non-BAL QSOs. (3) There is some indication that HIBAL QSOs as a class may be more polarized than non-BAL QSOs and therefore intermediate between LIBAL and non-BAL QSOs, but the statistics are not compelling from the sample surveyed thus far. (4) For LIBAL QSOs, the continuum polarization appears significantly correlated with the line profile detachment index, in the sense that LIBAL QSOs with P Cygni-type profiles are more polarized. No correlation was found with the strength of the low- or the high-ionization absorption features, nor with the strength or the width of the emission lines. These results are consistent with a scenario in which LIBAL QSOs constitute a different class of radio-quiet QSOs with more absorbing material and more dust. Higher maximum polarization can therefore be reached, while the actually measured polarization depends on the geometry and orientation of the system as do the line profiles. The observed correlation is interpreted within the framework of recent ``wind-from-disk'' models. Tables~2 and 3 are also available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html}\fnmsep\thanks{Based on observations collected at the European Southern Observatory (ESO, La Silla) [less ▲]

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See detailVariabilite cyclique d'etoiles Wolf-Rayet apparemment isolees.
Morel, Thierry ULg; St-Louis, N.

in Journal of the Royal Astronomical Society of Canada (1998), 92

Not Available

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See detailPhase behavior of diblock copolymers between styrene and n-alkyl methacrylates
Ruzette, A. V. G.; Banerjee, P.; Mayes, A. M. et al

in Macromolecules (1998), 31(24), 8509-8516

In contrast to most diblock copolymers which exhibit the classical upper critical ordering transition (UCOT), polystyrene-b-poly n-butyl methacrylatePS-b-PBMAhas been shown to undergo ordering upon ... [more ▼]

In contrast to most diblock copolymers which exhibit the classical upper critical ordering transition (UCOT), polystyrene-b-poly n-butyl methacrylatePS-b-PBMAhas been shown to undergo ordering upon heating through a lower critical ordering transition (LCOT). Here we report the phase behavior of a family of diblock copolymers formed from styrene and a homologous series of n-alkyl methacrylates, as determined by combined dynamic rheological testing and small-angle neutron scattering (SANS). It is shown that the shortest side chain methacrylates, with the exception of methyl methacrylate, exhibit the LCOT, while for side chains longer than n-butyl, the copolymers exhibit the classical UCOT behavior. Combined group contribution/lattice fluid model calculations of the solubility parameter and specific volume of the corresponding homopolymers qualitatively support these observations. The same calculations were further employed to molecularly design LCOT behavior into a new diblock material consisting of styrene and a random copolymer of methyl and lauryl methacrylate, denoted PS-b-P(MMA-r-LMA). The success of this approach suggests a simple semiquantitative method for predicting and designing the phase behavior of weakly interacting polymer pairs. [less ▲]

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See detailSpontaneous Apamin-Sensitive Hyperpolarizations in Dopaminergic Neurons of Neonatal Rats
Seutin, Vincent ULg; Massotte, Laurent ULg; Scuvée-Moreau, Jacqueline ULg et al

in Journal of Neurophysiology (1998), 80(6), 3361-4

Spontaneous apamin-sensitive hyperpolarizations in dopaminergic neurons of neonatal rats. J. Neurophysiol. 80: 3361-3364, 1998. Intracellular recordings from substantia nigra slices revealed the existence ... [more ▼]

Spontaneous apamin-sensitive hyperpolarizations in dopaminergic neurons of neonatal rats. J. Neurophysiol. 80: 3361-3364, 1998. Intracellular recordings from substantia nigra slices revealed the existence of spontaneous hyperpolarizations (amplitude 2-8 mV, duration 100-400 ms) at -60 mV in most dopaminergic neurons of neonatal (9-15 days) but not adult rats. These events were blocked by apamin (300 nM) and bicuculline methochloride (100-300 microM), which blocks apamin-sensitive currents. They were unaffected by the selective gamma-aminobutyric acid-A (GABAA) antagonists SR95531 (100 microM) and picrotoxin (30-50 microM), the GABAB antagonist CGP35348 (300 microM), the D2 antagonist haloperidol (1 microM), and the metabotropic antagonist MCPG (1 mM). The hyperpolarizations were strongly attenuated or abolished when recording electrodes contained 200 mM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. They were resistant to tetrodotoxin in the majority of the cells. They had some voltage dependency and were in some cases transiently potentiated when cells were briefly depolarized by current injection. We conclude that dopaminergic neurons have developmentally regulated physiological properties. These spontaneous hyperpolarizations might affect the firing rate of these cells, which was found to be lower in neonates than in adults. [less ▲]

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See detailSuivi du patient traité par amiodarone
LANCELLOTTI, Patrizio ULg; Melon, Pierre ULg; Kulbertus, Henri ULg

in Revue Médicale de Liège (1998), 53(12), 762-5

Amiodarone is a highly effective antiarrhythmic agent. Its iodine content and tissue accumulation are however responsible for various side effects. In this article, we review the various amiodarone ... [more ▼]

Amiodarone is a highly effective antiarrhythmic agent. Its iodine content and tissue accumulation are however responsible for various side effects. In this article, we review the various amiodarone related side effects and propose a monitoring chart for patients treated by this specific agent. [less ▲]

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See detailStereocomplexation of sPMMA-PBD-sPMMA triblock copolymers with isotactic PMMA. II: Effect of molecular weight
Yu, Jian Ming; Jérôme, Robert ULg

in Polymer (1998), 39(25), 6567-6575

Thermoplastic elastomers consisting of triblock copolymers (MBM) with outer syndiotactic poly(methyl methacrylate) (sPMMA) blocks associated to an inner polybutadiene (PBD) block have been modified by ... [more ▼]

Thermoplastic elastomers consisting of triblock copolymers (MBM) with outer syndiotactic poly(methyl methacrylate) (sPMMA) blocks associated to an inner polybutadiene (PBD) block have been modified by stereocomplexation with isotactic PMMA (iPMMA) at a constant 2/1 sPMMA/iPMMA (or s/i) mixing ratio. Toluene cast films of these stereocomplexes have been studied by thermal analysis as a function of molecular weight of the sPMMA blocks and the iPMMA homopolymer. Although the melting temperature of the stereocomplex (Tm) is independent of molecular weight of the sPMMA blocks ( sPMMA) in the studied range from 7000 to 46 000, the melting enthalpy increases with increasing sPMMA. This effect is, however, erased by the sample annealing at 140°C for 15 h, and a melting enthalpy of cα. 33 J/g total PMMA is then observed whatever the copolymer composition. One melting endotherm is observed in case of low sPMMA. although two melting endotherms are reported for higher sPMMA depending, however, on the heating rate. Two endotherms are indeed observed at small sPMMA as the heating rate is low. Molecular weight of iPMMA has no significant effect on either the melting temperature or the melting enthalpy of the stereocomplexes. Dynamic mechanical analysis has confirmed the phase separation of the complexed triblock copolymers. At constant molecular weight of PBD and iPMMA, stereocomplexation increases the tensile strength of MBM copolymers containing small sPMMA blocks, whereas the opposite effect is observed for longer sPMMA blocks. When the same MBM copolymer is complexed by iPMMA, the tensile properties are independent of the molecular weight of iPMMA at least in the range from 5000 to 74 000. [less ▲]

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See detailElectrochemically synthesized polypyrrole nanotubules : effects of different experimental conditions
Demoustier-Champagne, Sophie; Ferain, Etienne; Jérôme, Christine ULg et al

in European Polymer Journal (1998), 34(12), 1767-1774

Nanotubules of polypyrrole (PPy) were electrochemically synthesized using the pores of nanoporous polycarbonate (PC) particle track-etched membranes (PTM) as templates. The influence of some conditions of ... [more ▼]

Nanotubules of polypyrrole (PPy) were electrochemically synthesized using the pores of nanoporous polycarbonate (PC) particle track-etched membranes (PTM) as templates. The influence of some conditions of electrosynthesis (electrochemical method, monomer concentration, electrolyte concentration and nature) on the kinetics of pyrrole electropolymerization and on the morphology of the obtained nanomaterials has been investigated. In particular, the empirical kinetics of the electrochemical generation of polypyrrole-perchlorate (PPy-ClO4) from aqueous solution, at 0.8 V (vs SCE) was followed by electrical charges measurement. The orders with respect to pyrrole and LiClO4 are 0.34 and 0.70 respectively. The electrogeneration of polypyrrole-polystyrenesulfonate (PPy-PSS) was also followed and shows that an increase of the electrolyte concentration increases the polymerization rate at constant potential. Finally, the morphology of PPy nanotubules doped with LiClO4 and NaPSS has been studied by high resolution emission SEM and by TEM. [less ▲]

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See detailChlorophyll Synthesis in Dark-Grown Pine Primary Needles
Schoefs, B.; Franck, Fabrice ULg

in Plant Physiology (1998), 118(4), 1159-68

The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of ... [more ▼]

The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously demonstrated that pine primary needles are able to synthesize chlorophyll in the dark. Norflurazon strongly inhibited both carotenoid and chlorophyll synthesis. Needles of plants treated with this inhibitor had low chlorophyll content, contained only traces of xanthophylls, and accumulated carotenoid precursors. The first form of chlorophyll detected in young pine needles grown in darkness had an emission maximum at 678 nm. Chlorophyll-protein complexes with in situ spectroscopic properties similar to those of fully green needles (685, 695, and 735 nm) later accumulated in untreated plants, whereas in norflurazon-treated plants the photosystem I emission at 735 nm was completely lacking. To better characterize the light-dependent chlorophyll biosynthetic pathway in pine needles, the 77 K fluorescence properties of in situ protochlorophyllide a spectral forms were studied. Photoactive and nonphotoactive protochlorophyllide a forms with emission properties similar to those reported for dark-grown angiosperms were found, but excitation spectra were substantially red shifted. Because of their lower chlorophyll content, norflurazon-treated plants were used to study the protochlorophyllide a photoreduction process triggered by one light flash. The first stable chlorophyllide photoproduct was a chlorophyllide a form emitting at 688 nm as in angiosperms. Further chlorophyllide a shifts usually observed in angiosperms were not detected. The rapid regeneration of photoactive protochlorophyllide a from nonphotoactive protochlorophyllide after one flash was demonstrated. [less ▲]

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See detailMultimodular Penicillin-Binding Proteins: An Enigmatic Family of Orthologs and Paralogs
Goffin, Colette ULg; Ghuysen, Jean-Marie ULg

in Microbiology & Molecular Biology Reviews (1998), 62(4), 1079-1093

The monofunctional penicillin-binding DD-peptidases and penicillin-hydrolyzing serine beta-lactamases diverged from a common ancestor by the acquisition of structural changes in the polypeptide chain ... [more ▼]

The monofunctional penicillin-binding DD-peptidases and penicillin-hydrolyzing serine beta-lactamases diverged from a common ancestor by the acquisition of structural changes in the polypeptide chain while retaining the same folding, three-motif amino acid sequence signature, serine-assisted catalytic mechanism, and active-site topology. Fusion events gave rise to multimodular penicillin-binding proteins (PBPs). The acyl serine transferase penicillin-binding (PB) module possesses the three active-site defining motifs of the superfamily; it is linked to the carboxy end of a non-penicillin-binding (n-PB) module through a conserved fusion site; the two modules form a single polypeptide chain which folds on the exterior of the plasma membrane and is anchored by a transmembrane spanner; and the full-size PBPs cluster into two classes, A and B. In the class A PBPs, the n-PB modules are a continuum of diverging sequences; they possess a five-motif amino acid sequence signature, and conserved dicarboxylic amino acid residues are probably elements of the glycosyl transferase catalytic center. The PB modules fall into five subclasses: A1 and A2 in gram-negative bacteria and A3, A4, and A5 in gram-positive bacteria. The full-size class A PBPs combine the required enzymatic activities for peptidoglycan assembly from lipid-transported disaccharide-peptide units and almost certainly prescribe different, PB-module specific traits in peptidoglycan cross-linking. In the class B PBPs, the PB and n-PB modules cluster in a concerted manner. A PB module of subclass B2 or B3 is linked to an n-PB module of subclass B2 or B3 in gram-negative bacteria, and a PB module of subclass B1, B4, or B5 is linked to an n-PB module of subclass B1, B4, or B5 in gram-positive bacteria. Class B PBPs are involved in cell morphogenesis. The three motifs borne by the n-PB modules are probably sites for module-module interaction and the polypeptide stretches which extend between motifs 1 and 2 are sites for protein-protein interaction. The full-size class B PBPs are an assortment of orthologs and paralogs, which prescribe traits as complex as wall expansion and septum formation. PBPs of subclass B1 are unique to gram-positive bacteria. They are not essential, but they represent an important mechanism of resistance to penicillin among the enterococci and staphylococci. Natural evolution and PBP- and beta-lactamase-mediated resistance show that the ability of the catalytic centers to adapt their properties to new situations is limitless. Studies of the reaction pathways by using the methods of quantum chemistry suggest that resistance to penicillin is a road of no return. [less ▲]

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See detailDe quelques complexes, obsessions et refoulements en didactique du français langue étrangère et seconde
Defays, Jean-Marc ULg

in Langage et l'Homme (Le) : Recherches Pluridisciplinaires sur le Langage (1998), XXXIII(4), 451-463

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See detailEnantiomeric Separation of Pirlindole by Liquid Chromatography Using Different Types of Chiral Stationary Phases
Ceccato, Attilio ULg; Hubert, Philippe ULg; De Tullio, Pascal ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1998), 18(4-5), 605-14

The enantioseparation of pirlindole by liquid chromatography (LC) was investigated using three different chiral stationary phases (CSPs) containing either cellulose tris-(3,5-dimethylphenylcarbamate ... [more ▼]

The enantioseparation of pirlindole by liquid chromatography (LC) was investigated using three different chiral stationary phases (CSPs) containing either cellulose tris-(3,5-dimethylphenylcarbamate) (Chiralcel OD-R), ovomucoid (OVM) or beta-cyclodextrin (beta-CD). The effects of the mobile phase pH on retention, enantioselectivity and resolution were studied. Methanol and acetonitrile were tested as organic modifiers while the influence of the addition to the mobile phase of sodium alkanesulfonates or sodium perchlorate was also investigated. Sodium perchlorate was only used on the Chiralcel OD-R column while sodium alkanesulfonates were tested as mobile phase additives on the three kinds of CSPs. The enantioseparation of pirlindole could be obtained on all CSPs tested, the best results with respect to chiral resolution being achieved on the Chiralcel OD-R and the OVM columns. The use of sodium octanesulfonate (NaOS) was found to improve the enantioseparation of pirlindole on the OVM column while enantioselectivity was considerably enhanced by addition of sodium perchlorate on the Chiralcel OD-R column. [less ▲]

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See detailManagement of Recipients of Hepatic Allografts Harvested from Donors with Malignancy Diagnosed Shortly after Transplantation
Detry, Olivier ULg; Honore, Pierre ULg; Jacquet, Nicolas et al

in Clinical Transplantation (1998), 12(6), 579-81

Transmission of undiagnosed malignancy with the graft is a dramatic complication of liver transplantation. Alternatives in the management of the recipients of livers, harvested from donors with malignancy ... [more ▼]

Transmission of undiagnosed malignancy with the graft is a dramatic complication of liver transplantation. Alternatives in the management of the recipients of livers, harvested from donors with malignancy diagnosed shortly after transplantation, are either early re-transplantation or close follow-up without re-operation. We reported 4 cases of liver recipients whose allografts were harvested from donors who were diagnosed with malignancy shortly after the liver transplantation. One recipient underwent re-transplantation, and the three other allografts were not removed. No recipient developed recurrence in the follow-up. While graft removal may be the only way to avoid tumor recurrence in recipients of liver graft harvested from donor with malignancy, close follow-up without re-operation may also be considered. The risk of tumor transferral may depend on the histopathological aggressiveness and metastatic potential of the donor tumor, and may be low for low-grade, local tumors. This risk should be evaluated by analyzing large series, using databases of Eurotransplant or United Network for Organ Sharing. [less ▲]

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See detailJeunesse et violence à l'école: approche sociologique
Robert, Jocelyne ULg

in Education-Formation (1998), 252(hiver),

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See detailLe cinéma documentaire en Belgique francophone aujourd'hui
Van Cauwenberge, Geneviève ULg

in Revue Nouvelle (1998), 12

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See detailInternal functionalization in hyperbranched polyesters
Trollsas, Michaël; Hedrick, James L.; Mecerreyes, David et al

in Journal of Polymer Science. Part A, Polymer Chemistry (1998), 35(17), 3187-3192

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See detailTransplantation hépatique: expérience et résultats du programme de l'Université de Liège
Honore, Pierre ULg; Detry, Olivier ULg; Meurisse, Michel ULg et al

in Revue Médicale de Liège (1998), 53(12), 735-7

The orthotopic liver transplantation (OLT) program of the University of Liege was initiated in 1986. Between 1986 and December 1998, 150 adult OLT have been performed in our institution, including 18 ... [more ▼]

The orthotopic liver transplantation (OLT) program of the University of Liege was initiated in 1986. Between 1986 and December 1998, 150 adult OLT have been performed in our institution, including 18 liver retransplantations, 1 combined heart and liver transplantation and 3 combined liver and kidney transplantations. The aim of this study was to report the last 3 years of our experience. From January 1996 to November 1998, we performed 50 OLT on 49 patients. Three were retransplantations and two were combined liver and kidney transplantations. Fourty-three patients were transplanted for chronic liver disease and 6 for acute or subacute hepatopathy. Mean waiting time on the list was 4 weeks. Immunosuppression was based on triple therapy (cyclosporin A/tacrolimus, steroids, azathioprine), with steroid and azathioprine withdrawal in most of the patients after 3 months. In the chronic liver disease group, operative (< 30 days) survival was 95% (peroperative myocardial infarction in 2 patients). In the acute liver disease group, postoperative survival was 66%. No perioperative death occurred in 1997 and 1998. Actuarial one year survival was 87%. In our experience, OLT has become a safe procedure. [less ▲]

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See detailManagement of Fulminant Hepatic Failure
Detry, Olivier ULg; Honore, Pierre ULg; Meurisse, Michel ULg et al

in Acta Chirurgica Belgica (1998), 98(6), 235-40

Fulminant hepatic failure is an infrequent but dreadful disease, occurring usually in young patients. Despite fulminant hepatic failure is reversible in most of the cases, some patients develop brain ... [more ▼]

Fulminant hepatic failure is an infrequent but dreadful disease, occurring usually in young patients. Despite fulminant hepatic failure is reversible in most of the cases, some patients develop brain edema and intracranial hypertension, which are the most common cause of death in these patients. Liver transplantation significantly improves the prognosis of selected patients in who precise criteria predict a low chance of survival. This review summarizes the modern standard of care of patients with fulminant hepatic failure, with particular underlining of the management of brain oedema and intracranial hypertension. [less ▲]

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See detailEffects of Regular Insulin or Insulin Lispro on Glucose Metabolism after an Oral Glucose Load in Patients with Type 2 Diabetes Mellitus
Paquot, Nicolas ULg; Roulin, D.; Schneiter, P. et al

in Diabètes & Métabolism (1998), 24(6), 523-8

Seven obese Type 2 diabetic patients were studied for two 4-h periods after ingestion of a glucose load to determine the effects of preprandial subcutaneous injection of Insulin Lispro (5 min before the ... [more ▼]

Seven obese Type 2 diabetic patients were studied for two 4-h periods after ingestion of a glucose load to determine the effects of preprandial subcutaneous injection of Insulin Lispro (5 min before the meal) or regular insulin (20 min before the meal) on glucose metabolism. Glucose production and utilisation were measured using a dual isotope method. After Lispro, the mean postprandial increase in plasma glucose was 29% lower and the increase in insulin concentration 25% higher than after regular insulin (p < 0.05). Suppression of endogenous glucose production was similar with both types of insulin. Thus, preprandial injection of Lispro reduced postprandial glucose increments in Type 2 diabetic patients as compared to regular insulin. This effect is best explained by the increased postprandial bioavailability of Lispro. [less ▲]

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See detailPurification and Characterization of a 315 Kda Keratinolytic Subtilisin-Like Serine Protease from Microsporum Canis and Evidence of Its Secretion in Naturally Infected Cats
Mignon, Bernard ULg; Swinnen, M.; Bouchara, J. P. et al

in Medical Mycology (1998), 36(6), 395-404

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography ... [more ▼]

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids showed numerous similarities with other fungal subtilisins. The optimum pH was around 9 while inactivation of the enzyme was reversible at pH 4, but not at pH 11. The enzyme was stable at 37 degrees C with an apparent optimum temperature around 55 degrees C. PMSF, soybean trypsin inhibitor (SBTI) and chymostatin strongly inhibited the proteinase. The highest affinity (Km of 0.37 mM) and physiological efficiency (k(cat)/Km) were obtained for the synthetic substrate N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide. These results indicate that the keratinase belongs to the subtilisin-like serine protease family. Purified rabbit immunoglobulins G prepared against the keratinase and used in an immunohistochemical test allowed the detection of the keratinase produced by the fungus invading hair structures in naturally infected cats. The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity. [less ▲]

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See detailA propos des prothèses mammaires. L'examen senologique conventionnel
Lifrange, Eric ULg; Colin, Claude ULg

in Revue Médicale de Liège (1998), 53(12), 746-9

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See detailRecent developments in anionic polymerization
Jérôme, Robert ULg; Tong, Jiangdong

in Current Opinion in Solid State and Materials Science (1998), 3(6), 573-578

The past year witnessed very significant advances in the living anionic polymerization of (meth)acrylate monomers, particularly in hydrocarbons at or below 0°C. Block polymerization of alkyl methacrylates ... [more ▼]

The past year witnessed very significant advances in the living anionic polymerization of (meth)acrylate monomers, particularly in hydrocarbons at or below 0°C. Block polymerization of alkyl methacrylates with primary alkyl acrylates, although somewhat improved, remains a challenge. Anionic polymerization of styrene, diene and its derivatives was carried out with the aim of synthesizing functional polymers and block copolymers of various architectures. There has been a trend towards combining different living techniques in order to design polymers of unique architectures and properties. [less ▲]

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See detailEuropean multicentre evaluation of the Super Aution SA-4220 urinalysis analyser
Galimany, R.; Arambarri, M.; Biosca et al

in Clinical Chemistry & Laboratory Medicine (1998), 36(12), 947-58

A multicentre evaluation of the urine test strip analyser Super Aution-4220 was carried out in six laboratories. The analytical performance of the instrument with regard to imprecision, linearity ... [more ▼]

A multicentre evaluation of the urine test strip analyser Super Aution-4220 was carried out in six laboratories. The analytical performance of the instrument with regard to imprecision, linearity, detection limit, drift, carry-over and method comparison was studied. Using the Aution stick 8 test strip the pH, glucose, protein, ketones, bilirubin, blood, urobilinogen and leukocyte esterase were analysed. Specific gravity measurements were performed by refractive index method. Within-run and between-run imprecision determined at three levels of analyte were good. No carry-over was observed. Obtained results were linear through all the described analytical range. No significant drift was detected. Method comparison with some quantitative methods was performed and showed a good correlation with most of the analytes. The study of interferences showed minor interferences by common therapeutic drugs with the measurement of some analytes. During the assessment period of about 6 months no breakdown occurred in any laboratory. The Super Aution urine analyser appeared to be a highly automated analyser of urinary test strips. The operation was simple and the maintenance required only a few minutes a day. [less ▲]

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See detailPatterns of the Immunohistochemical Expression of Melanoma-Associated Antigens and Density of Cd45r0+ Activated T Lymphocytes and L1-Protein Positive Macrophages in Primary Cutaneous Melanomas
Pierard-Franchimont, Claudine ULg; Letawe, C.; Nikkels, Arjen ULg et al

in International Journal of Molecular Medicine (1998), 2(6), 721-724

There is ample evidence for spontaneous antimelanoma immune reactivity mediated by melanocyte-differentiation-antigens (MDAs). Our aim was to determine whether MDA immunoreactivity is associated with ... [more ▼]

There is ample evidence for spontaneous antimelanoma immune reactivity mediated by melanocyte-differentiation-antigens (MDAs). Our aim was to determine whether MDA immunoreactivity is associated with increased tumour-infiltrating lymphocytes (TIL) and macrophages (TIM). A retrospective study was conducted in 30 medium and high grade primary cutaneous melanomas (PCM) as identified by CART-analysis. All of the cases had developed clinical evidence for metastasis within 3 years following surgical excision of the PCM. We used immunohistochemistry and computerized image analysis to quantify MDAs positive cells (Melan A/MART-1, gp100/Pmel 17/HMB45, tyrosinase), CD45R0-positive TIL and LI-protein-positive TIM. A stochastic relationship was present between the MDA immuno-reactivities and the densities in TIL and TIM. An inverse relationship was yielded between TIL and TIM. No specific pattern of PCM immunoreactivity for MDAs, TIL and TIM was found to predict metastases. [less ▲]

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See detailHystéroscopie opératoire ou hystérectomie dans le traitement des lésions utérines bénignes. Que choisir en 1998?
Herman, Philippe ULg; Gaspard, Ulysse ULg; Foidart, Jean-Michel ULg

in Revue Médicale de Liège (1998), 53(12), 756-61

In the past, the treatment of benign uterine lesions required, in many instances, a hysterectomy. These days, most cases can be successfully treated by hysteroscopy. To be reliable, this technique must ... [more ▼]

In the past, the treatment of benign uterine lesions required, in many instances, a hysterectomy. These days, most cases can be successfully treated by hysteroscopy. To be reliable, this technique must lead to a significant reduction in the number of hysterectomies performed for benign uterine lesions. The electroresection technique is preferred to that using the Nd-YAG laser because of its lower cost and its equivalent efficacy. By using the uterine perfusion pump device, the risk of resorption syndrome can be reduced to its minimum. Submucosal myomas < 1 cm, benign endometrial hyperplasia and adenomyosis are the commonest benign lesions treated. Dysfunctional uterine bleeding can also be treated by an endometrectomy. A preoperative workup includes a transvaginal ultrasound and a biopsy. This ensures that only benign lesions that are accessible to a hysteroscopy will be submitted to this technique and that no cases of endometrial cancer or atypical hyperplasia would be ignored. This study presents 270 cases of operative hysteroscopy with a follow-up to 4 years. 82.8% of myomatous lesions were treated with success. The results for patients with benign endometrial polyps or benign endometrial hyperplasia are also excellent with only 4.6% and 5.6% rate of secondary surgery respectively. Adenomyosis does not appear to be a good indication for hysteroscopy as only 37% of patients did not need a definitive hysterectomy. Rates of operative complications (post-operative bleeding, uterine perforation, resorption syndrome and difficulty of access) are acceptable and get less frequent as the surgeon experience increases. [less ▲]

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See detailSuspension de l'exécution d'un permis d'urbanisme : conditions
Delnoy, Michel ULg

in Immobilier (1998), 20

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See detailRelative Reproductive success of males in polyandrous crosses of Barbus meridionalis (Cyprinidae) revealed by microsatellite genotyping
Chenuil, A.; Cattaneo-Berrebi, G.; Binda et al

in Folia Zoologica (1998), 47

Using microsatellite genotyping in Barbus meridionalis, we tried to assess the identity of fathers of progeny produced during four experimental polyandrous crosses involving the same four males and one or ... [more ▼]

Using microsatellite genotyping in Barbus meridionalis, we tried to assess the identity of fathers of progeny produced during four experimental polyandrous crosses involving the same four males and one or two females. The pooled analysis revealed that males had significantly different reproductive successes. We found no conspicuous causes explaining differential reproductive success in the behavioural data or in the physical characteristics of the males but a genetic determinism is the most parsimonious hypothesis to account for this variability. We argue that this conclusion is compatible with the hypothesis that the reproductive strategy of barbels, which involves large aggregations of males, is a result of sexual selection. This study provides guidelines for future interspecific crosses and constitutes the preliminary experiment of an investigation of the origin of a hybrid zone between Barbus barbus and Barbus meridionalis. [less ▲]

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See detailThree photometric methods tested on ground-based data of Q 2237+0305
Burud, I.; Stabell, R.; Magain, Pierre ULg et al

in Astronomy and Astrophysics (1998), 339

The Einstein Cross, Q 2237+0305, has been photometrically observed in four bands on two successive nights at NOT (La Palma, Spain) in October 1995. Three independent algorithms have been used to analyse ... [more ▼]

The Einstein Cross, Q 2237+0305, has been photometrically observed in four bands on two successive nights at NOT (La Palma, Spain) in October 1995. Three independent algorithms have been used to analyse the data: an automatic image decomposition technique, a CLEAN algorithm and the new MCS deconvolution code. The photometric and astrometric results obtained with the three methods are presented. No photometric variations were found in the four quasar images. Comparison of the photometry from the three techniques shows that both systematic and random errors affect each method. When the seeing is worse than 1farcs0 , the errors from the automatic image decomposition technique and the Clean algorithm tend to be large (0.04-0.1 magnitudes) while the deconvolution code still gives accurate results (1sigma error below 0.04) even for frames with seeing as bad as 1farcs7 . Reddening is observed in the quasar images and is found to be compatible with either extinction from the lensing galaxy or colour dependent microlensing. The photometric accuracy depends on the light distribution used to model the lensing galaxy. In particular, using a numerical galaxy model, as done with the MCS algorithm, makes the method less seeing dependent. Another advantage of using a numerical model is that eventual non-homogeneous structures in the galaxy can be modeled. Finally, we propose an observational strategy for a future photometric monitoring of the Einstein Cross. Based on observations obtained at NOT, La Palma. [less ▲]

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See detailLes effets de l'induction du travail sur le déroulement de l'accouchement
Gucciardo, L.; Thoumsin, H.; Foidart, Jean-Michel ULg

in Revue Médicale de Liège (1998), 53(11), 665-8

Labour induction has become a common practice. Given the significant number of induced deliveries carried out each year, it is important to know the consequences of this induction policy. The effects of ... [more ▼]

Labour induction has become a common practice. Given the significant number of induced deliveries carried out each year, it is important to know the consequences of this induction policy. The effects of labour induction on the childbirth are still the subject of considerable controversy. The increase of the caesarean and instrumental extraction rates has already been emphasized in previous studies. This article is based on an analysis of 10,045 deliveries carried out in the CHR Obstetrical Clinic of La Citadelle, between 1991 and 1997. The conclusions are instructive. Thus a departmental policy of setting an induction level of 35% will allocate 2/3 of the workload between 8:00 AM and 8:00 PM during the week, with a preponderance on Friday. The Caesarean rate is not increased but peridurals and recourse to instrumental extraction are more frequent. [less ▲]

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See detailEvaluation of a Diphenylphosphorylazide-Crosslinked Collagen Membrane for Guided Bone Regeneration in Mandibular Defects in Rats
Zahedi, Sharam ULg; Legrand, Roman ULg; Brunel, G. et al

in Journal of Periodontology (1998), 69(11), 1238-46

In the present study, the potential of a diphenylphosphorylazide-crosslinked type I bovine collagen membrane was evaluated in the healing of mandibular bone defects applying the biological concept of ... [more ▼]

In the present study, the potential of a diphenylphosphorylazide-crosslinked type I bovine collagen membrane was evaluated in the healing of mandibular bone defects applying the biological concept of guided bone regeneration. The experiment was carried out on 25 Wistar rats. After exposing the mandibular ramus bilaterally, 5 mm diameter full-thickness circular bone defects were surgically created. While the defect on one side was covered by the membrane (experimental), the defect on the other side was left uncovered (control) before closure of the overlying soft tissues. The rats were sacrificed in groups of 5 after 7, 15, 30, 90, and 180 days of healing. Although at early stages of healing similar amounts of bone formation were observed in the experimental and control defects, after 1 month of healing, most of the experimental defects were completely closed with new bone, while in the control defects, only limited amounts of new bone were observed at the rims and in the lingual aspect of the lesions. In the 90- and 180-day animals, all experimental defects were completely closed, while in the control defects, no statistically significant increase in bone regeneration was observed. The increase in percentage of bone regeneration in the experimental defects was statistically significant between the 15-day specimens as compared with the 7-day specimens (P < 0.01) and likewise between 30-day and 15-day specimens (P < 0.001). It can be concluded that a DPPA-crosslinked collagen membrane yields biocompatibility, ad hoc mechanical hindrance, and handling characteristics suitable for guided bone regeneration applications in this experimental model. [less ▲]

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See detailCultured Oligodendrocyte Progenitors Derived from Cerebral Cortex Express a Glycine Receptor Which Is Pharmacologically Distinct from the Neuronal Isoform
Belachew, Shibeshih ULg; Rogister, Bernard ULg; Rigo, Jean-Michel et al

in European Journal of Neuroscience (1998), 10(11), 3556-64

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also ... [more ▼]

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also triggered by beta-alanine and taurine, two established glycine receptor agonists. In our recording conditions, glycine-gated currents in oligodendrocyte progenitors reverse about 0 mV and are reversibly inhibited by the glycine competitive antagonist strychnine, the Cl- channel blocker picrotoxinin and the non-competitive antagonist cyanotriphenylborate. The oligodendrocyte progenitors glycine receptor (GlyR) differs from the corresponding neuronal receptor: [3H]strychnine binding data and the strychnine inhibition curve of glycine-induced currents in oligodendrocyte progenitor cultures suggest the existence of two strychnine binding sites on the oligodendroglial GlyR. Using total RNA isolated from oligodendrocyte progenitors cultures, reverse transcription-polymerase chain reaction analysis of glycine receptor subunit expression shows the presence of alpha2 and beta subunits and immunocytochemical stainings confirm that this GlyR contains an alpha subunit which is not alpha1. The molecular structure of the oligodendroglial GlyR could be either homopentameric alpha2 or heteromeric alpha2beta but in both cases, the sequence of the alpha2 or beta subunits have to be different from the known neuronal sequences in order to explain, respectively, the cyanotriphenylborate (alpha2) and picrotoxinin (beta) sensitivities. This work thus demonstrates that GlyR are expressed by oligodendrocytes obtained not only from spinal cord but also from supraspinal structures. The pharmacological properties and presumably the molecular structure of oligodendroglial GlyR are original. The physiological meaning of the presence of such receptors on developing and mature oligodendrocytes remains unknown. [less ▲]

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See detailMethod Development Strategies for the Enantioseparation of Drugs by Capillary Electrophoresis Using Cyclodextrins as Chiral Additives
Fillet, Marianne ULg; Hubert, Philippe ULg; Crommen, Jacques ULg

in Electrophoresis (1998), 19(16-17), 2834-40

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a ... [more ▼]

General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs were developed. For all kinds of compounds, the use of a buffer made of 100 mM phosphoric acid adjusted to pH 3 with triethanolamine and containing anionic and/or uncharged cyclodextrin (CD) derivatives as chiral selectors was recommended. Two different optimization schemes depending on the acidic or basic character of the analytes, were elaborated. For most basic compounds present in cationic form at pH 3, enantiomeric separation could be achieved in the normal polarity mode. Different beta-cyclodextrin derivatives were first tested at a given concentration. Five derivatives were found to be particularly useful for enantioseparations in capillary electrophoresis (CE): the anionic carboxymethyl-beta-CD (CMCD) and sulfobutyl-beta-CD (SBCD) and the neutral dimethyl-beta-CD (DMCD), trimethyl-beta-CD (TMCD) and hydroxypropyl-beta-CD (HPCD). After selection of the most suitable CD, its concentration was optimized with respect to chiral resolution. If necessary, a further improvement in resolution could often be obtained for the enantiomers of cationic solutes by increasing the buffer pH from 3 to 5 using CMCD as chiral additive. Another possible alternative for enhancement in chiral resolution was the addition of metharlol or cyclohexanol to the buffer. For acidic drugs, essentially present in uncharged form at pH 3, and for neutral solutes, anionic CD derivatives such as SBCD or CMCD were first tested at a given concentration in the reversed polarity mode. Dual systems, based on the simultaneous addition of a charged CD (SBCD or CMCD) and a neutral CD (TMCD or DMCD), could then be investigated for resolution improvement. After optimization of the CD concentrations, the use of dual systems with CMCD at pH 5 could also be tested if necessary, especially for very weak acidic and neutral drugs. By applying these optimization strategies, 48 of the 50 drugs examined as model compounds could be fully enantioseparated by CE in short analysis times (usually less than 10 min). [less ▲]

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See detailShape signifiers for throttle control of a low-order compressor model
Sepulchre, Rodolphe ULg; Kokotovic, Petar

in IEEE Transactions on Automatic Control (1998), 43(11), 1643-1648

Rotating stall and surge, two instability mechanisms limiting the performance of aeroengines compressors, are studied on the third-order Moore–Greitzer model. The skewness of the compressor characteristic ... [more ▼]

Rotating stall and surge, two instability mechanisms limiting the performance of aeroengines compressors, are studied on the third-order Moore–Greitzer model. The skewness of the compressor characteristic, a single parameter shape signifier, is shown to determine the key qualitative properties of feedback control. [less ▲]

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See detail“Sainte” : le double procès du texte
Durand, Pascal ULg

in Op. Cit. : Revue de Littératures Française et Comparée (1998), 11

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See detailMorphometric Study of the Equine Navicular Bone: Variations with Breeds and Types of Horse and Influence of Exercise
Gabriel, Annick ULg; Jolly, Sandra ULg; Detilleux, Johann ULg et al

in Journal of Anatomy (1998), 193((Pt 4)), 535-49

Navicular bones from the 4 limbs of 95 horses, classified in 9 categories, were studied. The anatomical bases were established for the morphometry of the navicular bone and its variations according to the ... [more ▼]

Navicular bones from the 4 limbs of 95 horses, classified in 9 categories, were studied. The anatomical bases were established for the morphometry of the navicular bone and its variations according to the category of horse, after corrections were made for front or rear limb, sex, weight, size and age. In ponies, navicular bone measurements were smallest for light ponies and regularly increased with body size, but in horses, navicular bone dimensions were smallest for the athletic halfbred, intermediate for draft horse, thoroughbreds and sedentary halfbreds and largest for heavy halfbreds. The athletic halfbred thus showed reduced bone dimensions when compared with other horse types. Navicular bones from 61 horses were studied histomorphometrically. Light horses and ponies possessed larger amounts of cancellous bone and less cortical bone. Draft horses and heavy ponies showed marked thickening of cortical bone with minimum intracortical porosity, and a decrease in marrow spaces associated with more trabecular bone. Two distinct zones were observed for the flexor surface cortex: an external zone composed mainly of poorly remodelled lamellar bone, disposed in a distoproximal oblique direction, and an internal zone composed mainly of secondary bone, with a lateromedial direction for haversian canals. Flexor cortex external zone tended to be smaller for heavy ponies than for the light ponies. It was the opposite for horses, with the largest amount of external zone registered for draft horses. In athletic horses, we observed an increase in the amount of cortical bone at the expense of cancellous bone which could be the result of reduced resorption and increased formation at the corticoendosteal junction. Cancellous bone was reduced for the athletic horses but the number of trabeculae and their specific surfaces were larger. Increased bone formation and reduced resorption could also account for these differences. [less ▲]

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See detailJuvenile Rheumatoid Arthritis and Del(22q11) Syndrome: A Non-Random Association
Verloes, Alain ULg; Curry, C.; Jamar, Michelle ULg et al

in Journal of Medical Genetics (1998), 35(11), 943-7

Del(22q11) is a common microdeletion syndrome with an extremely variable phenotype. Besides classical manifestations, such as velocardiofacial (Shprintzen) or DiGeorge syndromes, del(22q11) syndrome may ... [more ▼]

Del(22q11) is a common microdeletion syndrome with an extremely variable phenotype. Besides classical manifestations, such as velocardiofacial (Shprintzen) or DiGeorge syndromes, del(22q11) syndrome may be associated with unusual but probably causally related anomalies that expand its phenotype and complicate its recognition. We report here three children with the deletion and a chronic, erosive polyarthritis resembling idiopathic cases of juvenile rheumatoid arthritis (JRA). Patient 1, born in 1983, initially presented with developmental delay, facial dysmorphism, velopharyngeal insufficiency, and severe gastro-oesophageal reflux requiring G tube feeding. From the age of 3 years, he developed JRA, which resulted in severe restrictive joint disease, osteopenia, and platyspondyly. Patient 2, born in 1976, had tetralogy of Fallot and peripheral pulmonary artery stenosis. She developed slowly, had mild dysmorphic facial features, an abnormal voice, and borderline intelligence. JRA was diagnosed at the age of 5 years. The disorder followed a subacute course, with relatively mild inflammatory phenomena, but an extremely severe skeletal involvement with major osteopenia, restrictive joint disease (bilateral hip replacement), and almost complete osteolysis of the carpal and tarsal bones with phalangeal synostoses, leading to major motor impairment and confinement to a wheelchair. Patient 3, born in 1990, has VSD, right embryo-toxon, bifid uvula, and facial dysmorphism. She developed JRA at the age of 1 year. She is not mentally retarded but has major speech delay secondary to congenital deafness inherited from her mother. In the three patients, a del(22q11) was shown by FISH analysis. These observations, and five other recently published cases, indicate that a JRA-like syndrome is a component of the del(22q11) spectrum. The deletion may be overlooked in those children with severe, chronic inflammatory disorder. [less ▲]

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See detailElectrografting of acrylic and methacrylic monomers onto metals: influence of the relative polarity and donor-acceptor properties of the monomer and the solvent
Baute, Noëlle; Teyssié, Philippe; Martinot, Lucien et al

in European Journal of Inorganic Chemistry (1998), 1998(11), 1711-1720

The possibility of grafting a series of alkyl polyacrylates and polymethacrylates onto a nickel cathode by electropoly-merization of the parent monomers has been investigated and has emphasized the ... [more ▼]

The possibility of grafting a series of alkyl polyacrylates and polymethacrylates onto a nickel cathode by electropoly-merization of the parent monomers has been investigated and has emphasized the critical importance of the solvent used. Indeed, the intensity of the inhibition peak, which is the electrochemical mark of the cathode passivation as result of the polymer grafting, clearly depends on both the polarity and the donor-acceptor properties of the solvent. The Gutmann concept is used to account for these experimental results. An increase in the donicity of the solvent used for the electrochemical medium has allowed, for the very first time, several polyacrylates and polymethacrylates [such as poly(ethyl acrylate), poly(methyl methacrylate), and poly(2-trimethylsilyloxyethyl methacrylate)] to be electrografted onto Ni. This observation is consistent with a competition process between the monomer and the solvent for being adsorbed on the cathode and amassing in its very close vicinity. The outcome of this competition is controlled by the relative polarity (in case of low donicity) and the relative donor-acceptor properties (when the difference is high enough) of the monomer/solvent pair, and by the monomer concentration (in case of weak competition). A semiquantitative relationship has also been observed between the monomer ability to be electrografted and the electron-accepting character of the vinyl β-carbon atom as measured by 13C NMR. [less ▲]

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See detailVaricella-zoster virus IE63, a virion component expressed during latency and acute infection, elicits humoral and cellular immunity
Sadzot-Delvaux, Catherine ULg; Arvin, Ann M.; Rentier, Bernard ULg

in Journal of Infectious Diseases (1998), 178(Suppl. 1), 43-47

Varicella-zoster virus (VZV) latency in human dorsal root ganglia is characterized by the transcription of large regions of its genome and by the expression of large amounts of some polypeptides, which ... [more ▼]

Varicella-zoster virus (VZV) latency in human dorsal root ganglia is characterized by the transcription of large regions of its genome and by the expression of large amounts of some polypeptides, which are also expressed during lytic cycles. The immediate early 63 protein (IE63) is a virion component expressed very early in cutaneous lesions and the first viral protein detected during latency. Immune response against IE63 has been evaluated among naturally immune adults with a history of chickenpox: Specific antibodies were detected in serum, and most subjects who had a T cell proliferation with unfractionated VZV antigens had T cell recognition of purified IE63. The cytotoxic T cell (CTL) response to IE63 was equivalent to CTL recognition of IE62, the major tegument component of VZV, whose immunogenicity has been previously described. T cell recognition of IE63 and other VZV proteins is one of the likely mechanisms involved in controlling VZV reactivation from latency. [less ▲]

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See detailLaminar difference in GABA uptake and GAT-1 expression in rat CA1
Engel, Dominique ULg; Schmitz, D; Gloveli, T et al

in Journal of Physiology (1998), 512(Pt3), 643-649

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See detailCrossing of two mobile averages: A method for measuring the roughness exponent
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1998), 58(5), 6832-6834

Self-affine signals characterized by a defined Hurst (roughness) exponent H can be investigated through mobile averages. The density rho of crossing points between any two moving averages is a measure of ... [more ▼]

Self-affine signals characterized by a defined Hurst (roughness) exponent H can be investigated through mobile averages. The density rho of crossing points between any two moving averages is a measure of long-range power-law correlations in the signal. The method is compared to the detrended fluctuation analysis. We rake advantage of our findings in order to propose a practically powerful and accurate technique for determining H and apply it right away to cases with persistent or antipersistent correlations. [less ▲]

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See detailTransplantation d'un rein prélevé laparoscopiquement chez un donneur vivant apparente
Detry, Olivier ULg; Defechereux, Thierry ULg; Hamoir, Etienne ULg et al

in Revue Médicale de Liège (1998), 53(11), 657-9

Transplantation of kidney grafts harvested in living donors has demonstrated better results than grafts harvested from brain dead donors. Recently, laparoscopic live donor nephrectomy has been introduced ... [more ▼]

Transplantation of kidney grafts harvested in living donors has demonstrated better results than grafts harvested from brain dead donors. Recently, laparoscopic live donor nephrectomy has been introduced to reduce the live procurement morbidity. [less ▲]

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See detailFgf-3 and Fgf-4 Elicit Distinct Oncogenic Properties in Mouse Mammary Myoepithelial Cells
Hajitou, Amin; Baramova, Eugénia; Bajou, Khalid ULg et al

in Oncogene (1998), 17(16), 2059-71

Fibroblast Growth Factors 3 (FGF-3) and 4 (FGF-4) were compared for the effects they each exert on EF43 mouse cells. This non-transformed mammary cell line appears to be myoepithelial mainly because it ... [more ▼]

Fibroblast Growth Factors 3 (FGF-3) and 4 (FGF-4) were compared for the effects they each exert on EF43 mouse cells. This non-transformed mammary cell line appears to be myoepithelial mainly because it expresses alpha-smooth muscle actin. The EF43 cells were infected with similar vectors that carry either the short fgf-3 sequence (the product of which goes into the secretory pathway), fgf-4 or the selection gene only as control. In syngeneic animals, EF43.fgf-3 cells were tumorigenic only when orthotopically implanted whereas EF43.fgf-4 cells invariably gave rise to aggressive tumors. However, both tumor types were metastatic as evidenced by the blue micrometastases observed when the implanted cells expressed lacZ. In vitro, the FGF-3 producing cells were strongly invasive in matrigel coated chambers whereas the EF43.fgf-4 cells only were invasive in type I-collagen gels. Interestingly, FGF-3 production greatly stimulated the synthesis of pro-MMP-9 (Matrix Metalloprotease-9) and, to a lesser extent, that of pro-MMP-2. FGF-3 also up-regulated the production of plasminogen activators. In contrast, FGF-4 had no effect on these secretions and the medium conditioned by the EF43.fgf-4 cells displayed the largest plasminogen activator-inhibitor activity. These results show that FGF-3 and FGF-4 have distinct mechanisms of action on myoepithelial cells. [less ▲]

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See detailTransmission and confocal fluorescence microscopy and time-resolved fluorescence spectroscopy combined with a laser trap: investigation of optically trapped block copolymer micelles
Gensch, Thomas; Hofkens, Johan; van Stam, Jan et al

in Journal of Physical Chemistry B (1998), 102(43), 8440-8451

Optical trapping was combined with transmission microscopy (TM), confocal and nonconfocal fluorescence scanning microscopy (CFSM and FSM, respectively), and confocal and nonconfocal time-resolved ... [more ▼]

Optical trapping was combined with transmission microscopy (TM), confocal and nonconfocal fluorescence scanning microscopy (CFSM and FSM, respectively), and confocal and nonconfocal time-resolved fluorescence spectroscopy (CTRFS and TRFS, respectively) to study latex particles and block copolymer micelles. Dye-labeled latex particles of various size, in polymer composite films as well as optically trapped in solution, were studied with CFSM to characterize the limits of the setup. CFSM revealed that the resolution in the x- and y-directions was near the theoretical limit, i.e., 200−250 nm. CTRFS on the labeled latex particles revealed that the decay time of the label was not influenced by the polymer matrix nor the optical trap. Poly(tert-butylstyrene-block-sodium methacrylate) micelles (diameter approximately 30−40 nm) in deuterated aqueous solutions could be optically trapped, this region of high copolymer micelle concentration being referred to as a trapped cluster. In the transmission images, trapped clusters of 1.5−2 μm diameter were detected. Fluorescence images were obtained using perylene as a fluorophore that is specifically dissolved within the block copolymer micelles. The size of the trapped cluster, estimated from TM and FSM images, increases with increasing irradiation time and power, respectively. In the TM images, the trapped cluster appears as a dark spot (low transmission) with a bright (high transmission) corona-like ring around it. The appearance of the corona is explained as a light deflection phenomenon; i.e., the trapped cluster acts as lens due to a lateral refractive index gradient. When the corona is taken into account when the diameter of the trapped clusters is calculated, a very good agreement is found between TM and FSM. Long irradiation times lead to the formation of large trapped clusters, which are stable for about 10 s, with diameters of several hundred nanometers, while, for short irradiation times, the trapped cluster is smaller and disappears within a time less than 1 s. With CFSM it could be shown that the trapped particle has a spot size of approximately 1.7 μm in the region of the IR laser focus, while the diameter extends up to 5 μm without using the confocal imaging capability. The reason for this is that the conditions for optical trapping are fulfilled not only in but also above and below the focal region. Due to the high numerical aperture, a dumbbell-like shape of the trapped cluster results. [less ▲]

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See detailInvestigation of methyl methacrylate and vinyl acetate polymerization promoted by Al(iBu)3/2,2'-bipyridine and Al(iBu)3/2,2'-bipyridine/TEMPO complexes
Granel, Claude; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1998), 31(21), 7133-7141

Results of methyl methacrylate (MMA) and vinyl acetate (VOAc) polymerization using the Al(iBu)3/2,2‘-bipyridine/TEMPO initiating system at room temperature failed to confirm the “living” radical mechanism ... [more ▼]

Results of methyl methacrylate (MMA) and vinyl acetate (VOAc) polymerization using the Al(iBu)3/2,2‘-bipyridine/TEMPO initiating system at room temperature failed to confirm the “living” radical mechanism reported earlier. Several species apparently propagate without control in a normal free radical process and only above a critical initiator concentration. No evidence was found for the penta- and hexacoordinated aluminum species previously suggested, but high conversion to an alkoxyamine was observed. Additionally, mixing Al(iBu)3 and BIPY promotes a variety of reactions involving alkyl and hydride transfers to the aromatic rings forming reduced BIPY products, along with a persistent organoaluminum radical. All these results illustrate that the process is considerably more complicated than postulated before. Conversely, at −78 °C, a single active species excluding the participation of TEMPO has been put in evidence, displaying some “living” characteristics. Several experiments support its anionic nature, as previously suggested by Ikeda. [less ▲]

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See detailTnf-Alpha and Ifn-Gamma Down-Regulate the Expression of the Metastasis-Associated Bi-Functional 37lrp/P40 Gene and Protein in Transformed Keratinocytes
Clausse, Nathalie; van den Brûle, Frédéric; Delvenne, Philippe ULg et al

in Biochemical and Biophysical Research Communications (1998), 251(2), 564-9

The 37 LRP/p40 molecule is a bi-functional protein in which expression is increased in a large variety of cancers in association with their metastatic phenotype. Here we present the first data concerning ... [more ▼]

The 37 LRP/p40 molecule is a bi-functional protein in which expression is increased in a large variety of cancers in association with their metastatic phenotype. Here we present the first data concerning the 37 LRP/p40 gene promoter activity and show that it is very active in a cervix carcinoma cell line. Interestingly, despite hallmarks of a housekeeping gene, we show that the 37 LRP/p40 gene promoter can be down-regulated by two potentially anticancerous cytokines, TNF-alpha and IFN-gamma. In addition, the dual fate of the protein, i.e., being intracellularly involved in the cell translation machinery and incorporated into a 67-kDa cell surface protein functioning as a laminin receptor (67LR), is differentially affected by the treatment. Our data suggest multiple regulation levels in the control of the 67LR/37LRP/p40 molecule expression and uncover new clues for the understanding of both the control of expression of this metastasis-associated molecule and the IFN-gamma and TNF-alpha anticancerous action. [less ▲]

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See detailCarbon Dioxide Emission from European Estuaries
Frankignoulle, Michel; Abril, Gwenaël; Borges, Alberto ULg et al

in Science (1998), 282(5388), 434-6

The partial pressure of carbon dioxide (pCO2) in surface waters and related atmospheric exchanges were measured in nine European estuaries. Averaged fluxes over the entire estuaries are usually in the ... [more ▼]

The partial pressure of carbon dioxide (pCO2) in surface waters and related atmospheric exchanges were measured in nine European estuaries. Averaged fluxes over the entire estuaries are usually in the range of 0.1 to 0.5 mole of CO2 per square meter per day. For wide estuaries, net daily fluxes to the atmosphere amount to several hundred tons of carbon (up to 790 tons of carbon per day in the Scheldt estuary). European estuaries emit between 30 and 60 million tons of carbon per year to the atmosphere, representing 5 to 10% of present anthropogenic CO2 emissions for Western Europe. [less ▲]

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See detailPositive parity pentaquarks in a Goldstone boson exchange model
Stancu, Floarea ULg

in Physical Review. D : Particles and Fields (1998), D58

We study the stability of the pentaquarks $uudd\overline{Q}$, $uuds\overline{Q}$ and $udss\overline{Q}$ ($Q = c$ or $b$) of positive parity in a constituent quark model based on Goldstone boson exchange ... [more ▼]

We study the stability of the pentaquarks $uudd\overline{Q}$, $uuds\overline{Q}$ and $udss\overline{Q}$ ($Q = c$ or $b$) of positive parity in a constituent quark model based on Goldstone boson exchange interaction between quarks. The pentaquark parity is the antiquark parity times that of a quark excited to a p-shell. We show that the Goldstone boson exchange interaction favors these pentaquarks much more than the negative parity ones of the same flavour content but all quarks in the ground state. We find that the nonstrange pentaquarks are stable against strong decays. [less ▲]

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See detailAcromegalie : Onderzoeken
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 51

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See detailX-ray analysis of the NMC-A beta-lactamase at 1.64-A resolution, a class A carbapenemase with broad substrate specificity
Swaren, Peter; Maveyraud, Laurent; Raquet, Xavier et al

in Journal of Biological Chemistry (1998), 273(41), 26714-26721

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See detailAcromégalie : Examens complémentaires
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 51

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See detail4-Methoxypyridine N-Oxide: a new regulator for the controlled free radical polymerization of methyl methacrylate
Detrembleur, Christophe ULg; Lecomte, Philippe ULg; Caille, Jean-Raphaël et al

in Macromolecules (1998), 31(20), 7115-7117

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See detailAcromégalie : Diagnostic différentiel
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 50

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See detailAcromegalie : Differentiaal-diagnose
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 50

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See detailElectrochemical synthesis of polypyrrole nanowires
Jérôme, Christine ULg; Jérôme, Robert ULg

in Angewandte Chemie (International ed. in English) (1998), 37(18), 2488-2490

Through a hole in a poly(ethyl acrylate) (PEA) layer that is electrochemically grafted to the surface of a vitreous carbon electrode-that is the route that must be taken by a growing polypyrrole nanowire ... [more ▼]

Through a hole in a poly(ethyl acrylate) (PEA) layer that is electrochemically grafted to the surface of a vitreous carbon electrode-that is the route that must be taken by a growing polypyrrole nanowire in the electropolymerization of pyrrole. Chain growth is controlled by diffusion of the monomer through the DMF-swollen PEA layer, which acts as a template for the formation of nanowires (shown in the picture) with diameters of 400-1000 nm and lengths of up to 300 m. [less ▲]

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See detailCyclical Spectral and Photometric Variations of the Apparently Single Wolf-Rayet Star WR 134
Morel, Thierry ULg; Marchenko, S. V.; Eenens, P. R. J. et al

in Astrophysics & Space Science (1998), 260

Evidence is presented for the existence of a 2.3 day periodicity in the line-profile changes of the apparently singleWolf-Rayet star WR 134. This cyclical variability may be induced either by the presence ... [more ▼]

Evidence is presented for the existence of a 2.3 day periodicity in the line-profile changes of the apparently singleWolf-Rayet star WR 134. This cyclical variability may be induced either by the presence of an orbiting collapsed companion, or by the rotational modulation of a largely inhomogeneous outflow. [less ▲]

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See detailMolecular Cloning of a Mutated Hoxb7 Cdna Encoding a Truncated Transactivating Homeodomain-Containing Protein
Chariot, Alain ULg; Senterre-Lesenfants, Sylviane; Sobel, Mark E et al

in Journal of Cellular Biochemistry (1998), 71(1), 46-54

Homeodomain-containing proteins regulate, as transcription factors, the coordinated expression of genes involved in development, differentiation, and malignant transformation. We report here the molecular ... [more ▼]

Homeodomain-containing proteins regulate, as transcription factors, the coordinated expression of genes involved in development, differentiation, and malignant transformation. We report here the molecular cloning of a mutated HOXB7 transcript encoding a truncated homeodomain-containing protein in MCF7 cells. This is a new example of mutation affecting the coding region of a HOX gene. In addition, we detected two HOXB7 transcripts in several breast cell lines and demonstrated that both normal and mutated alleles were expressed at the RNA level in MCF7 cells as well as in a variety of breast tissues and lymphocytes, suggesting that a truncated HOXB7 protein might be expressed in vivo. Using transient co-transfection experiments, we demonstrated that both HOXB7 proteins can activate transcription from a consensus HOX binding sequence in breast cancer cells. Our results provide evidence that HOXB7 protein has transcription factor activity in vivo and that the two last amino acids do not contribute to this property. [less ▲]

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See detailPharma clinics. Comment je traite ... ou préviens une douleur par "patches"
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(10), 586-7

There exists two distinct types of patches aiming at increasing the pain threshold either systematically or locally. The first type of analgesic patch is a real transdermal delivery system releasing ... [more ▼]

There exists two distinct types of patches aiming at increasing the pain threshold either systematically or locally. The first type of analgesic patch is a real transdermal delivery system releasing fentanyl. Such potent opioid drug exerts a systemic effect. The other type of patch associates lidocain and prilocain to induce a local anesthesia of the skin without inducing a systemic effect. [less ▲]

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See detailStadsverkeer: problemen en olpossingen
Marchal, Jean ULg

in Publication du CAWET (Comité van de Academie voor Wetenschappen en Techniek) (1998)

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See detailExpression and Modulation of Homeobox Genes from Cluster B in Endothelial Cells
Belotti, D.; Clausse, Nathalie; Flagiello, D. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1998), 78(10), 1291-9

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the ... [more ▼]

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in organ development and tissue differentiation. In this study, we have demonstrated that human umbilical vein endothelial cells (HUVEC) express 8 of the 10 HOX genes contained in cluster B. Treatment of HUVEC with tissue plasminogen activator (TPA), an agent known to induce morphologic changes in endothelial cells, or vascular endothelium growth factor (VEGF), a proliferative and angiogenesis inducer, results in a specific time-dependent modulation of the eight HOX genes identified. Interestingly, neither basic fibroblast growth factor, an endothelial proliferative agent, nor TNP-470, a fumagillin derivative with potent antiendothelial cell proliferation properties, affected expression of these HOX genes. Specific modulation of HOX genes by differentiating agents but not by proliferative or antiproliferative molecules suggests that they could be involved in the control of the genetic program that coordinates the construction of new blood vessels. [less ▲]

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See detail18fdg-Pet for the Assessment of Primary Head and Neck Tumors: Clinical, Computed Tomography, and Histopathological Correlation in 38 Patients
Paulus, Patrick; Sambon, A.; Vivegnis, Danielle et al

in Laryngoscope (1998), 108(10), 1578-83

OBJECTIVES: To evaluate the clinical usefulness of FDG-PET (fluoro-2-deoxy-glucose-positron emission tomography) in the detection of lymph node involvement and recurrences in patients with head and neck ... [more ▼]

OBJECTIVES: To evaluate the clinical usefulness of FDG-PET (fluoro-2-deoxy-glucose-positron emission tomography) in the detection of lymph node involvement and recurrences in patients with head and neck cancer. STUDY DESIGN: Retrospective review of 38 patients with biopsy-proven head and neck cancers who underwent clinical, computed tomography (CT), and FDG-PET examinations. Twenty-five patients were studied prior to therapy and 13 patients were evaluated for disease recurrence. METHODS: All patients were operated and clinical data, CT, and FDG-PET results were correlated with histopathological findings. RESULTS: All primary tumors in 25 patients were detected, with the exception of one small superficial localization of the epiglottis. Histopathological examination showed lymph node involvement in 10 patients; PET detected lymph node involvement in five. FDG-PET found one case of nodal disease not identified by clinical and CT examination. With so few cases, this could be anecdotal. Five false-negative results (microscopic lymph node involvement) and two false positives were noted. Twelve of 13 patients with recurrent disease were correctly identified with FDG-PET. FDG-PET was the only imaging technique to identify local recurrence in two patients and lymph node involvement in two others. One false-positive result occurred in a patient with a foreign body granuloma. CONCLUSIONS: FDG-PET is a useful diagnostic modality for the detection of recurrent tumors and, in selected cases, precise lymph node involvement. The best way to further investigate the utility of clinical FDG-PET is in the follow-up of treated patients. [less ▲]

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See detailEfficacy and Tolerability of Calcitonin in the Prevention and Treatment of Osteoporosis
Halkin, V; Reginster, Jean-Yves ULg

in BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals & Gene Therapy (1998), 10(4), 295-300

Calcitonin in general, and, more specifically, salmon calcitonin (salcatonin), has been known for 30 years to be a specific inhibitor of bone resorption. Studies have confirmed its efficacy in metabolic ... [more ▼]

Calcitonin in general, and, more specifically, salmon calcitonin (salcatonin), has been known for 30 years to be a specific inhibitor of bone resorption. Studies have confirmed its efficacy in metabolic bone diseases characterised by excessive bone resorption, such as osteoporosis. Most randomised studies in which salcatonin and oral calcium were administered for 1 to 5 years to recently postmenopausal women for the prevention of osteoporosis have shown that bone mineral density or bone content of the lumbar spine increased significantly, compared with a reduction among women receiving calcium only. Prospective studies have shown that salcatonin is effective in the treatment of established osteoporosis, reducing significantly the relative risk of new vertebral fractures. The benefits of salcatonin nasal spray therapy were observed in the majority of women studied, and it has been shown to be an effective alternative for osteoporotic women more than 5 years postmenopausal who refuse estrogens, or for whom estrogens are contraindicated. Finally, in established osteoporosis, nasal calcitonin possesses a potent analgesic effect. The well-demonstrated effects of nasal calcitonin permit it to be considered a well tolerated and efficient approach for prevention and treatment of postmenopausal osteoporosis. [less ▲]

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See detailConception des ouvrages hydrauliques
Rigo, Philippe ULg

in Ingénieur et Industrie, APPS éditions, n°5 (1998)

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See detailFoam-like evolution in polycrystalline systems following successive 'melt and growth' cycles
Vandewalle, Nicolas ULg; Delisse, B.; Ausloos, Marcel ULg et al

in Philosophical Magazine B-Physics of condensed matter statistical mechanics electronic optical and magnetic properties (1998), 78(4), 397-408

A stochastic model of multigrain growth which allows for successive melting-growth cycles is investigated on a square lattice. Two fundamental constraints are introduced: (i) the melted mass amplitude and ... [more ▼]

A stochastic model of multigrain growth which allows for successive melting-growth cycles is investigated on a square lattice. Two fundamental constraints are introduced: (i) the melted mass amplitude and (ii) the number of cycles guide the process. The evolution of the microstructure is found to be quite similar to foam systems, i.e. topological rearrangements are observed together with the increase of the-mean grain area. A drastic crossover between two types of growth regimes is found as a function of the amplitude of the melting-growth cycles. This allows one to envisage the existence of optimal conditions for polycrystal processing. [less ▲]

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See detailLe matérialisme transcendantal
Seba, Jean-Renaud ULg

in Cahiers marxistes (1998)

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See detailLa troponine, nouveau marqueur de l'infarctus du myocarde
Chapelle, Jean-Paul ULg

in Revue Médicale de Liège (1998), 53(10), 619-24

The troponin (Tn) complex consists of three protein subunits referred to as TnT, TnI and TnC. Myocardium contains TnI and TnT isoforms which are not present in skeletal muscles and which can be separated ... [more ▼]

The troponin (Tn) complex consists of three protein subunits referred to as TnT, TnI and TnC. Myocardium contains TnI and TnT isoforms which are not present in skeletal muscles and which can be separated from the muscular isoforms by immunological techniques. Using commercially available immunoassays, clinical laboratories are able to determine cardiac TnT and TnI (cTnT and cTnI) as quickly and reliably as classical cardiac markers. After acute myocardial infarction, cTnT and cTnI concentrations start to increase in serum in a rather similar way than CK-MB, but return to normal after longer periods of time (approximately one week). Because of their excellent cardiac specificity, Tn subunits appear ideally suited for the differential diagnosis of myocardial and muscular damage, for example in non cardiac surgery patients, in patients with muscular trauma or with chronic muscular diseases, or after intensive physical exercise. cTnT and cTnI may also be used for detecting evidence of minor myocardial damage, and therefore may find new applications in the management of patients with unstable angina. [less ▲]

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See detailIn Vitro Propagated Dendritic Cells from Patients with Human-Papilloma Virus-Associated Preneoplastic Lesions of the Uterine Cervix: Use of Flt3 Ligand
Hubert, Pascale ULg; Greimers, Roland ULg; Franzen-Detrooz, E. et al

in Cancer Immunology, Immunotherapy (1998), 47(2), 81-9

Dendritic cells (DC) are the most efficient antigen presenting cells. The clinical use of DC as vectors for antitumor and anti-infectious disease immunotherapy has been limited by their low level and ... [more ▼]

Dendritic cells (DC) are the most efficient antigen presenting cells. The clinical use of DC as vectors for antitumor and anti-infectious disease immunotherapy has been limited by their low level and accessibility in normal tissue. Substantial numbers of DC can be generated from peripheral blood cultured in the presence of interleukin-4 (IL-4) and granulocyte/macrophage-colony-stimulating factor (GM-CSF). We showed in this study that substantial numbers of DC can be obtained from the peripheral blood of patients with (pre)neoplastic lesions of the uterine cervix. The procedure required relatively small blood samples (10 ml) and the presence of 100 U/ml IL-4 and 800 U/ml GM-CSF in the culture medium. There was no significant difference in the morphology, yield, phenotype and function of generated DC between patients with cervical (pre)neoplastic lesions and healthy individuals. When the hematopoietic factor Flt3 ligand (Flt3L, 40 ng;ml) was added, there was an average increase in the DC population of 26% compared to cultures with GM-CSF and IL-4 alone. Approximately 1.2 x 10(6) cells with the characteristics of dendritic cells could be obtained when Flt3L was included in the medium. The addition of Flt3L did not modify the phenotypic profile of DC (HLA-DR+, CD1a+, CD4+, CD54+, CD80+, CD86+. CD40+, CD3- and CD14-). In addition, Flt3L generated functional DC capable of stimulating the proliferation of alloreactive T cells. These results suggest that Flt3L, in association with GM-CSF and IL-4, provides an advantageous tool for the large-scale generation of DC and that an immunotherapy based on the use of DC generated in vitro is possible in patients with (pre)neoplastic lesions of the uterine cervix. [less ▲]

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See detailRestriction of mobility in model liquid crystalline ionomers
Gohy, Jean-François; Jérôme, Robert ULg; Van den Bossche, Guy ULg et al

in Macromolecular Chemistry and Physics (1998), 199(10), 2205-2210

Model liquid crystalline ionomers have been analyzed by SAXS both in bulk and in toluene solution. These compounds are referred to as liquid crystalline halatosemitelechelic polystyrenes (LC HSTP's) and ... [more ▼]

Model liquid crystalline ionomers have been analyzed by SAXS both in bulk and in toluene solution. These compounds are referred to as liquid crystalline halatosemitelechelic polystyrenes (LC HSTP's) and consist of low molecular weight polystyrene chains end-capped at one end by a sulfonate group associated to a mesogenic counterion. The results are in agreement with a microphase separation between the polymer chain and the mesogenic counterion. The main characteristic feature of the SAXS profile for the bulk material is the so-called ionic peak, whose position obeys a general law which is in favor of a rod-like organization of the multiplets and which shows that the polymer chains are stretched in the very close vicinity of the mesogenic core, in agreement with the Eisenberg, Hird and Moore model for ionomers. The thickness of this region of restricted mobility has been estimated to 1 nm, which is the order of magnitude of the persistence length of polystyrene. This very local restriction in mobility persists in toluene solution, in contrast to what is observed for the ω-Li-sulfonato-polystyrene precursors. The results also agree with an isotropic distribution of the multiplets in toluene. [less ▲]

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See detailAn unusual pituitary pathology
Petrossians, Patrick ULg; Delvenne, Philippe ULg; Flandroy, P. et al

in Journal of Clinical Endocrinology and Metabolism (1998), 83(10), 3454-3458

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See detailThe burden of illness of hypopituitary adults with growth hormone deficiency.
Hakkaart-van Roijen, L.; Beckers, Albert ULg; Stevenaert, Achille ULg et al

in PharmacoEconomics (1998), 14(4), 395-403

Objective: The negative metabolic and psychosocial consequences of growth hormone deficiency (GHD) in adults are now well established. In the present study, an attempt was made to quantify the burden of ... [more ▼]

Objective: The negative metabolic and psychosocial consequences of growth hormone deficiency (GHD) in adults are now well established. In the present study, an attempt was made to quantify the burden of illness, in terms of lost productivity and increased medical consumption, associated with hypopituitarism and untreated GHD. Design and Setting: The study population consisted of 129 Belgian adults with untreated GHD associated with hypopituitarism after pituitary surgery. The Short-Form 36 Health Survey (SF-36) was used to assess health status, and the Health and Labour Questionnaire was used to measure production losses and labour performance. Data on medical consumption were also collected. Main Outcome Measures and Results: Hypopituitary patients reported a lower health status than that of the general population in all but two dimensions of the SF-36 (pain and physical functioning). Nearly 11% of the patients reported being incapacitated for paid employment due to health problems, compared with 4.8% of the general Belgian population. Patients in paid employment reported a mean of 19.8 days of sickness leave per year, which is twice that in the general population. The annual number of visits to general practitioners and specialists was also higher in the patients (9.6 and 6.5 visits, respectively, for the patients compared with corresponding figures of 2.1 and 1.5 for the general Belgian population). The average annual number of days spent in hospital was 3.5 for the patients compared with 2.3 in the general population. The annual healthcare costs and costs due to production losses calculated for hypopituitary patients who had received pituitary surgery amounted to 135 024 Belgian francs (BeF) or $US4340 (1995 values). This compares with the mean annual cost per person for the Belgian population as a whole of BeF68 569 or $US2204. Conclusions: Hypopituitary patients with untreated GHD therefore have a higher cost to society in terms of lost production and medical consumption than the average Belgian population. [less ▲]

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See detailA new theory for torsional stiffness of multi-span bundle overhead transmission lines.
Wang, Jianwei; Lilien, Jean-Louis ULg

in IEEE Transactions on Power Delivery (1998), 13(N°4), 1405-1411

A new theory for torsional stiffness of multi-span bundle overhead lines is presented.

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See detailLe cas clinique du mois. L'endocardite métastatique: observation clinique et revue de la littérature
Graas, M.P.; Evrard, P.; CREEMERS, Etienne ULg et al

in Revue Médicale de Liège (1998), 53(10), 588-91

The authors describe a case of metastatic endocarditis associated with a gastric carcinoma. The diagnosis was made early and the treatment by surgery and chemotherapy allowed a survival of 18 months ... [more ▼]

The authors describe a case of metastatic endocarditis associated with a gastric carcinoma. The diagnosis was made early and the treatment by surgery and chemotherapy allowed a survival of 18 months, which is unusually long. The differential diagnosis is discussed and includes nonbacterial thrombotic endocarditis, infectious endocarditis and primary tumors of the heart. [less ▲]

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See detailPrevalence and Molecular Typing of Attaching and Effacing Escherichia Coli among Calf Populations in Belgium
China, B.; Pirson, V.; Mainil, Jacques ULg

in Veterinary Microbiology (1998), 63(2-4), 249-259

Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in ... [more ▼]

Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in microvilli effacement, (ii) the production of the intimin, a 94 kDa outer membrane protein encoded by the eaeA gene and involved in the intimate attachment of bacteria to epithelial cell and (iii) the production of verotoxins: VT1 and/or VT2. We investigated the presence and the pathotype of these strains in several calf populations by colony hybridization or by genetic amplification. Using the colony hybridization method we showed first that only 5% of calves who died from diarrhea presented EaeA+ E. coli strains and secondly that 19% of healthy calves showed an asymptomatic carriage. However, using colony hybridization and genetic amplification, we identified EaeA+ strains in 91% of calves living in farms with recurrent diarrhea problems. In 66% of the calves, there was a correlation between the presence of AEEC and diarrhea. At the pathotype level, most of the EaeA+ isolates were negative for VT probes. In VT+ bacteria, the majority were VT1+. The number of VT positive bacteria was significantly higher in calves who died from diarrhea than in healthy or sick calves. This underlined the aggravating role of verotoxins in the disease. Moreover, only 25% of the bovine AEEC were positive with the EaeB probe. Surprisingly, the proportion of EaeB+ strains was significantly higher in healthy calves than in other populations. [less ▲]

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See detailAge-related differences in random generation
Van der Linden, Martial ULg; Beerten, A.; Pesenti, M.

in Brain & Cognition (1998), 38(1), 1-16

This study investigated the effects of age on a random generation task. In Experiment 1, young and elderly subjects were asked to generate random strings of letters at 1-, 2-, and 4-s rates. The elderly ... [more ▼]

This study investigated the effects of age on a random generation task. In Experiment 1, young and elderly subjects were asked to generate random strings of letters at 1-, 2-, and 4-s rates. The elderly subjects produced more alphabetical stereotype responses than young subjects, even in the slowest rate condition. Furthermore, as faster rates were imposed, elderly subjects could no longer maintain the pace and missed responses. In Experiment 2, subjects were required to generate letters at the same time that they sorted cards into one, two, four, or eight categories. Age-related differences were observed on most of the measures of randomness (stereotypes, zero-order, and first-order measures). In addition, the number of errors increased with the number of sorting alternatives, especially for elderly subjects. These results suggest the existence of a reduction of the central executive resources, along with a reduced inhibition ability, in the elderly subjects. However, the contribution of a perceptual speed factor is also discussed. [less ▲]

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See detailL'image du mois. Embolisation utérine d'une tumeur trophoblastique persistante
Foidart, Jean-Michel ULg; Henroteaux, D.; Schaaps, Jean-Pierre ULg

in Revue Médicale de Liège (1998), 53(10), 585

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See detailClinical Pattern of Corticosteroid Dependent Crohn's Disease
Franchimont, D. P.; Louis, Edouard ULg; Croes, F. et al

in European Journal of Gastroenterology & Hepatology (1998), 10(10), 821-5

OBJECTIVES: Corticosteroid dependency in Crohn's disease (CD) is characterized by the need for chronic use of corticosteroids to maintain clinical remission. Several definitions have been used. Depending ... [more ▼]

OBJECTIVES: Corticosteroid dependency in Crohn's disease (CD) is characterized by the need for chronic use of corticosteroids to maintain clinical remission. Several definitions have been used. Depending on the definition, 10-30% of the patients are considered as corticosteroid dependent. The aim of the study was to define a clinical pattern of corticosteroid dependent CD patients. PATIENTS AND METHODS: Epidemiological and clinical characteristics were retrospectively compared between 20 corticosteroid dependent CD patients and 248 non-corticosteroid dependent CD patients by using univariate and multivariate analyses. Corticosteroid dependency was defined either by two successive relapses during the 2 months after discontinuing glucocorticoids (n=5) or by two successive relapses at dose tapering, after successful treatment of a flare-up by using glucocorticoids (n=15). RESULTS: Corticosteroid dependent CD patients were younger at diagnosis (P < 0.001), and were characterized by a higher frequency of colonic location (P< 0.05), but lower frequency of ileal location (P < 0.01), and higher ano-perineal location (P < 0.05). They were also more frequently smokers (P< 0.05) and users of contraceptive pills (P< 0.01). The inflammatory type of the disease was increased (P < 0.01), while the fibrostenotic type was decreased (P < 0.001) in corticosteroid dependent CD patients. By multivariate analysis, a smoking habit (P < 0.01), a colonic location (P < 0.05), a non-fibrostenotic type (P< 0.05) and a younger age at diagnosis (P< 0.05) were shown to be independently associated with corticosteroid dependency. CONCLUSIONS: This study suggests a clinical pattern associated with corticosteroid dependency. Whether this clinical pattern is simply associated with the dependency, or whether a primary decrease of corticosensitivity produces this clinical pattern, is not known. Further prospective studies will have to determine whether the presence of these clinical characteristics is predictive of corticosteroid dependency and whether this prediction will be useful for the management of this condition. [less ▲]

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See detailAcromégalie : Consultation
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 49

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See detailAcromegalie : Consult
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 49

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See detailInfluence of end group and molecular weight on polybutadiene fingerprint secondary ion mass spectra
Vanden Eynde, X.; Bertrand, P.; Dubois, Philippe ULg et al

in Macromolecules (1998), 31(19), 6409-6416

Polybutadiene samples of different molecular weight have been synthesized by anionic polymerization as initiated by sec-butyllithium with low polydispersity and a major content of 1,2-vinyl units. They ... [more ▼]

Polybutadiene samples of different molecular weight have been synthesized by anionic polymerization as initiated by sec-butyllithium with low polydispersity and a major content of 1,2-vinyl units. They have been analyzed by time-of-flight secondary ion mass spectrometry (ToF-SIMS) in order to investigate the sensitivity of this method toward the sec-butyl end group and toward the molecular weight. The SIMS spectra show the characteristic fragment of the end group, C4H9+ at m/z = 57, whose the peak intensity is strongly dependent on the polymer molecular weight, as is the case for almost all the fragment intensities. A model consistent with the peak intensity variations is used to give some new insights into the fragmentation mechanism at the end groups and within the main chain. Moreover, the analysis of the end group fragment allows Mn to be readily determined up to Mn = 4 × 104 from, for example, the Y(53)/Y(57) intensity ratio where Y(53) is the intensity of the deprotonated repeat unit ([M − H]+). Other Mn calibration methods have also been used and are discussed in terms of their accuracy and physical meaning. [less ▲]

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See detailDecreased Protein Levels of the C-Cbl Protooncogene in Murine Aids
Trebak, Mohamed; Lambert, Charles A; Rahmouni, Souad ULg et al

in Cellular Immunology (1998), 188(2), 151-7

Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do ... [more ▼]

Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do not participate in the process. The 120-kDa protooncogene product c-Cbl was initially cloned from the murine Cas NS-1 B cell lymphoma. It is a main target of immunoreceptor (TCR and BCR)-mediated protein tyrosine kinase activity. Moreover, recent data suggest that c-Cbl might play a crucial role in the regulation of cell proliferation through regulation of GTP-binding proteins. Therefore, the involvement of c-Cbl was evaluated in the lymphoproliferative disease induced by the MAIDS virus. The expression of the c-Cbl protein was dramatically reduced in the lymph node of infected mice while it remained normal in the thymus. In contrast, the expression of actin, TCR-zeta chain, ZAP-70, and p59(fyn) remained similar in controls and infected mice. Identical results were obtained with sorted B cells and T cells. Surprisingly, a B cell lymphoma line derived from late stage MAIDS mice displayed a normal level of c-Cbl. [less ▲]

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See detailSimultaneous Determination of Methylphenobarbital Enantiomers and Phenobarbital in Human Plasma by on-Line Coupling of an Achiral Precolumn to a Chiral Liquid Chromatographic Column
Ceccato, Attilio ULg; Boulanger, Bruno ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (1998), 819(1-2), 143-53

A fully automated liquid chromatographic (LC) method for the simultaneous determination of methylphenobarbital enantiomers and phenobarbital in human plasma has been developed. The method is based on the ... [more ▼]

A fully automated liquid chromatographic (LC) method for the simultaneous determination of methylphenobarbital enantiomers and phenobarbital in human plasma has been developed. The method is based on the use of a precolumn packed with an internal-surface reversed-phase packing material (LiChrospher ADS) for sample clean-up coupled to LC analysis on a cellulose tris(4-methylbenzoate) based chiral stationary phase (Chiralcel OJ-R). A 100-microliter plasma sample was injected directly on the precolumn packed with LiChrospher RP-18 ADS using a mixture of pH 5.0 phosphate buffer-methanol (97:3, v/v) as washing liquid. The analytes were then eluted in the back-flush mode with the LC mobile phase. The enantiomeric separation of methylphenobarbital was achieved on Chiralcel OJ-R). The retention times were modelled using a D-optimal design with ten experimental points in order to optimise the LC mobile phase for the separation of phenobarbital from the enantiomers of mephobarbital. The factors selected were the acetonitrile content, the pH and the sodium perchlorate concentration in the mobile phase. A Derringer's desirability function was used to find an optimal and robust solution within the experimental domain. The mobile phase selected consisted of a mixture of pH 7.0 phosphate buffer-acetonitrile (60:40, v/v). The elution profiles of phenobarbital, methylphenobarbital and blank plasma samples on the precolumn and the time needed for analyte transfer from the precolumn to the analytical column were then determined. Finally, the method developed was validated. [less ▲]

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See detailSimultaneous Determination of Pirlindole Enantiomers and Dehydropirlindole by Chiral Liquid Chromatography
Ceccato, Attilio ULg; Hubert, Philippe ULg; De Tullio, Pascal ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1998), 17(6-7), 1071-9

Liquid chromatography was employed for the determination of pirlindole enantiomers and its oxidation product dehydropirlindole (DHP). The direct separation of pirlindole enantiomers and DHP was achieved ... [more ▼]

Liquid chromatography was employed for the determination of pirlindole enantiomers and its oxidation product dehydropirlindole (DHP). The direct separation of pirlindole enantiomers and DHP was achieved on a cellulose tris-(3,5-dimethylphenylcarbamate) chiral stationary phase (Chiralcel OD-R). Acetonitrile was used as the organic modifier and sodium perchlorate was used as an ionic additive in the mobile phase. The influence of acetonitrile and sodium perchlorate concentrations on enantioselectivity and achiral selectivity towards DHP was investigated in order to find suitable conditions for the determination of low amounts of each analyte. The mobile phase selected consisted of a mixture of acetonitrile and phosphate buffer (pH 5.0) containing sodium perchlorate (0.05 M) (35:65, v/v) and the UV detector was set at 220 nm. The method developed was validated and was found to be linear in the 0.1-5 microg ml(-1) range (r2 = 0.999 for the three compounds). Repeatability and the intermediate precision for the three analytes at a concentration of 0.1 microg ml(-1) were about 3 and 4%, respectively. This concentration corresponds to the quantification of 0.1% for the minor enantiomer. Actual determinations of enantiomeric purity for single enantiomers of pirlindole were performed. [less ▲]

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See detailEffects of Brain Testosterone Implants on Appetitive and Consummatory Components of Male Sexual Behavior in Japanese Quail
Riters, L. V.; Absil, Philippe ULg; Balthazart, Jacques ULg

in Brain Research Bulletin (1998), 47(1), 69-79

Aromatization of testosterone (T) into an estrogen is necessary for the activation of consummatory and appetitive sexual behavior in male Japanese quail. T action within the medial preoptic nucleus (POM ... [more ▼]

Aromatization of testosterone (T) into an estrogen is necessary for the activation of consummatory and appetitive sexual behavior in male Japanese quail. T action within the medial preoptic nucleus (POM) is necessary and sufficient to activate consummatory behavior, and some evidence suggests that POM might be involved in the control of appetitive behavior, but other brain regions, such as the bed nucleus of the stria terminalis (BST), an area that contains a dense population of aromatase-immunoreactive neurons, are also likely to be involved. This study was performed to assess the effects of stereotaxic T implants targeting either the POM or the BST on the activation of both components of sexual behavior in castrated male quail. Appetitive sexual behavior was measured by an acquired social proximity response in which a male will approach a window providing visual access to a female after the window has been repeatedly paired with physical access to a female and the possibility to freely interact with her. Rhythmic cloacal sphincter movements that are produced by the male when given visual access to a female were used as another measure of appetitive sexual behavior that does not appear to depend on sexual learning. The experiments confirmed that copulation is necessary for males to develop the social proximity response that is used to measure the appetitive sexual behavior. T implants in the POM activated both components of sexual behavior, suggesting that these components cannot be completely dissociated. In contrast, T implants located within the BST did not affect either component, but because implants in the BST did not activate copulatory behavior, these results do not preclude a role for BST in the expression of a previously acquired appetitive sexual behavior. [less ▲]

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See detailHubble Space Telescope imaging of Jupiter's UV aurora during the Galileo orbiter mission
Clarke, John T; Ben Jaffel, Lotfi; Gérard, Jean-Claude ULg

in Journal of Geophysical Research (1998), 103

Hubble Space Telescope (HST) Wide-Field Planetary Camera 2 (WFPC 2) images of Jupiter's aurora have been obtained close in time with Galileo ultraviolet spectrometer (UVS) spectra and in situ particles ... [more ▼]

Hubble Space Telescope (HST) Wide-Field Planetary Camera 2 (WFPC 2) images of Jupiter's aurora have been obtained close in time with Galileo ultraviolet spectrometer (UVS) spectra and in situ particles, fields, and plasma wave measurements between June 1996 and July 1997, overlapping Galileo orbits G1, G2, G7, G8, and C9. This paper presents HST images of Jupiter's aurora as a first step toward a comparative analysis of the auroral images with the in situ Galileo data. The WFPC 2 images appear similar to earlier auroral images, with the main ovals at similar locations to those observed over the preceding 2 years, and rapidly variable emissions poleward of the main ovals. Further examples have been observed of the equatorward surge of the auroral oval over 140-180° longitude as this region moves from local morning to afternoon. Comparison of the WFPC 2 reference auroral ovals north and south with the VIP4 planetary magnetic field model suggests that the main ovals map along magnetic field lines exceeding 15R[SUB]J[/SUB], and that the Io footprint locations have lead angles of 0-10° from the instantaneous magnetic projection. There was an apparent dawn auroral storm on June 23, 1996, and projections of the three dawn storms imaged with HST to date demonstrate that these appear consistently along the WFPC 2 reference oval. Auroral emissions have been consistently observed from Io's magnetic footprints on Jupiter. Possible systematic variations in brightness are explored, within factor of 6 variations in brightness with time. Images are also presented marked with expected locations of any auroral footprints associated with the satellites Europa and Ganymede, with localized emissions observed at some times but not at other times. [less ▲]

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See detailL'Obésité chez l'enfant.
Guillaume, Michèle ULg; Burniat, W.

in ONE (1998)

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See detailBIG LUCKY CARTER - Richard HITE - LEROY MARTIN
Sacré, Robert ULg

in Soul Bag (1998), (152), 25

Photo : Richard HITE, Leroy MARTIN et Big Lucky CARTER ( Memphis, TN, avril 1985)

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