References of "Scientific journals"
     in
Bookmark and Share    
Full Text
See detailQuelques données objectives sur la désignation des forêts dans les sites Natura 2000
Claessens, Hugues ULg; Lecomte, H.; Rondeux, Jacques ULg

in Forêt Wallonne (2004), (69-70), 42-47

Detailed reference viewed: 35 (12 ULg)
Full Text
Peer Reviewed
See detailNew radioiodinated carboxylic and hydroxamic matrix metalloproteinase inhibitor tracers as potential tumor imaging agents
Oltenfreiter, R.; Staelens, L.; Lejeune, Annabelle ULg et al

in Nuclear Medicine & Biology (2004), 31(4), 459-468

Several studies have demonstrated a positive correlation between tumor progression and expression of extracellular proteinases such as matrix metalloproteinases (MMPs). MMP-2 and MMP-9 have become ... [more ▼]

Several studies have demonstrated a positive correlation between tumor progression and expression of extracellular proteinases such as matrix metalloproteinases (MMPs). MMP-2 and MMP-9 have become attractive targets for cancer research because of their increased expression in human malignant tumor tissues of various organs, providing a target for medical imaging techniques. Radioiodinated carboxylic and hydroxamic MMP inhibitors 2-(4'-[123]iodo-biphenyl-4-sulfonylainino)-3-(1H-indol-3-yl)-propionic acid (9) and 2-(4'-[I-123] iodo-biphenyl-4-sulfonylamino)-3-(1H-indol-3-yl)-propionamide (11) were synthesized by electrophilic aromatic substitution of the tributylstannyl derivatives and resulted in radiochemical yields of 60% +/- 5% (n - 3) and 70% +/- 5% (n = 6), respectively. In vitro zymography and enzyme assays showed high inhibition capacities of the inhibitors on gelatinases. In vivo biodistribution showed no long-terin accumulation in organs and the possibility to accumulate in the tumor. These results warrant further studies of radioiodinated carboxylic and hydroxamic MNIP inhibitor tracers as potential SPECT tumor imaging agents. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailEndothelin-1 is a critical mediator of myogenic tone in tumor arterioles: implications for cancer treatment.
Sonveaux, Pierre; Dessy; MARTINIVE, Philippe ULg et al

in Cancer Research (2004), 1(64), 3209-14

Although derived from the host tissue, the tumor vasculature is under the influence of the tumor microenvironment and needs to adapt to the resistance to blood flow inherent to the dynamics of tumor ... [more ▼]

Although derived from the host tissue, the tumor vasculature is under the influence of the tumor microenvironment and needs to adapt to the resistance to blood flow inherent to the dynamics of tumor growth. Such vascular remodeling can offer selective targets to pharmacologically modulate tumor perfusion and thereby improve the efficacy of conventional anticancer treatments. Radiotherapy and chemotherapy can, indeed, take advantage of a better tumor oxygenation and drug delivery, respectively, both partly dependent on the tumor blood supply. Here, we showed that isolated tumor arterioles mounted in a pressure myograph have the ability, contrary to size-matched healthy arterioles, to contract in response to a transluminal pressure increase. This myogenic tone was exquisitely dependent on the endothelin-1 pathway because it was completely abolished by the selective endothelin receptor A (ETA) antagonist BQ123. This selectivity was additionally supported by the large increase in endothelin-1 abundance in tumors and the higher density of the ETA receptors in tumor vessels. We also documented by using laser Doppler microprobes and imaging that administration of the ETA antagonist led to a significant increase in tumor blood flow, whereas the perfusion in control healthy tissue was not altered. Finally, we provided evidence that acute administration of the ETA antagonist could significantly stimulate tumor oxygenation, as determined by electron paramagnetic resonance oximetry, and increase the efficacy of low-dose, clinically relevant fractionated radiotherapy. Thus, blocking the tumor-selective increase in the vascular endothelin-1/ETA pathway led us to unravel an important reserve of vasorelaxation that can be exploited to selectively increase tumor response to radiotherapy. [less ▲]

Detailed reference viewed: 56 (8 ULg)
Full Text
Peer Reviewed
See detailL'attaque de panique
Scantamburlo, Gabrielle ULg; Ansseau, Marc ULg

in Revue Médicale de Liège (2004), 59(5), 293-6

Panic attacks can occur unexpectedly and in almost any situation. The present article discusses the main features of anxiety states, the approach, the management and the practice guidelines for the ... [more ▼]

Panic attacks can occur unexpectedly and in almost any situation. The present article discusses the main features of anxiety states, the approach, the management and the practice guidelines for the treatment of panic disorder. [less ▲]

Detailed reference viewed: 152 (8 ULg)
Full Text
Peer Reviewed
See detailRolling and slipping motion of Euler's disk
Caps, Hervé ULg; Dorbolo, Stéphane ULg; Ponte, S. et al

in Physical Review E (2004), 69(5, Pt 2), 56610

We present an experimental study of the motion of a circular disk spun onto a table. With the help of a high speed video system, the temporal evolution of (i) the inclination angle alpha, (ii) the angular ... [more ▼]

We present an experimental study of the motion of a circular disk spun onto a table. With the help of a high speed video system, the temporal evolution of (i) the inclination angle alpha, (ii) the angular velocity omega, and (iii) the precession rate Omega are studied. The influence of the mass of the disk as well as the friction between the disk and the supporting surface are considered. Both inclination angle and angular velocity are observed to decrease according to a power law. We also show that the precession rate diverges as the motion stops. Measurements are performed very near the collapse as well as on long range times. Times to collapse have been also measured. Results are compared with previous theoretical and experimental works. The major source of energy dissipation is found to be the slipping of the disk on the plane. [less ▲]

Detailed reference viewed: 70 (5 ULg)
Full Text
Peer Reviewed
See detailHippopotamus of the W-Arli-Pendjari-Oti-Mandouri-Keran Ecosystem. West Africa. Status, distribution and conservation issues
Bouché, Philippe ULg

in Suiform soundings PPHSG Newsletter (2004), 4(1), 14-19

For the first time in April 2003, an aerial total wildlife count of the entire W-Arli-Pendjari-Oti- Mandouri-Keran (WAPOK) Ecosystem, the largest savannah ecosystem of West Africa, has been conducted ... [more ▼]

For the first time in April 2003, an aerial total wildlife count of the entire W-Arli-Pendjari-Oti- Mandouri-Keran (WAPOK) Ecosystem, the largest savannah ecosystem of West Africa, has been conducted. During this survey, Hippopotamus have been observed 51 times, totaling 696 individuals. An average of 13.86 ± 16.73 individuals was noted. The group size varied between 1 and 65 individuals. In the WAPOK ecosystem, the future of hippopotamus is ensured by the tourism activity in the area and the redistribution of tourism revenue to human communities living around the ecosystem, thanks to several conservation project, but more certainly, to the conservation initiative of several private stakeholders. [less ▲]

Detailed reference viewed: 51 (7 ULg)
Full Text
Peer Reviewed
See detailLa syncope
Mathieu, F.; Melon, Pierre ULg; Waleffe, André ULg et al

in Revue Médicale de Liège (2004), 59(5), 311-4

Syncope remains a clinical challenge. Accurate history taking, physical examination and EKG are mainstays of the diagnosis work up. The most important parameter for prognosis and requiring aggressive ... [more ▼]

Syncope remains a clinical challenge. Accurate history taking, physical examination and EKG are mainstays of the diagnosis work up. The most important parameter for prognosis and requiring aggressive management is a structural heart disease. Patient without cardiopathy presenting multiple episodes may be candidate for tilt testing and loop EKG recorder. [less ▲]

Detailed reference viewed: 24 (3 ULg)
Full Text
Peer Reviewed
See detailLes émulsifiants dans les produits de boulangerie industrielle : modes d'action, rôles et utilisations.
Delacharlerie, Sophie ULg; Blecker, Christophe ULg; Kegelaers, Yves et al

in Industries Alimentaires et Agricoles (2004), (Mai), 8-14

Detailed reference viewed: 180 (9 ULg)
Full Text
Peer Reviewed
See detailContributions of benthic and planktonic primary producers to nitrate and ammonium uptake fluxes in a nutrient-poor shallow coastal area (Corsica, NW Mediterranean)
Lepoint, Gilles ULg; Gobert, Sylvie ULg; Dauby, Patrick ULg et al

in Journal of Experimental Marine Biology and Ecology (2004), 302(1), 107-122

By using the stable isotope N-15, we have measured in situ the uptake of nitrate and ammonium by the seagrass Posidonia oceanica, its leaf epiphyte community, the brown macroalgae Halopteris scoparia and ... [more ▼]

By using the stable isotope N-15, we have measured in situ the uptake of nitrate and ammonium by the seagrass Posidonia oceanica, its leaf epiphyte community, the brown macroalgae Halopteris scoparia and the suspended particulate organic matter (SPOM). In Revellata Bay (Gulf of Calvi, Westem Corsica), which is a very nutrient-poor region, the specific uptake rates (V) (mug N g N 1 h(- 1)) of SPOM measured at ambient concentrations are 10-1000 higher than those of benthic primary producers. Macroalgae have intermediary v, between the seagrass leaf and leaf epiphytes. V are quite variable and the reasons for this variability remain unclear. Despite the difference of specific uptake rates found between benthic and pelagic primary producers, when integrating the uptake fluxes for a water Column of 10 m depth, the contribution of benthic primary producers to N uptake fluxes (g N m(-) (2) h(-) (1)) is significant, corresponding on average to 40% of total uptake flux. This results from the dominance in terms of N biomass of benthic primary producers in this shallow nutrient-poor area. When reported for the entire volume of the Revellata Bay, the contribution of benthic primary producers is reduced to 5 - 10% of total N uptake flux. Although this contribution could appear relatively low, it results in a significant direct transfer of inorganic nitrogen from the water column to the benthic compartment. By this transfer, the benthic plants act as a biological pump incorporating the pelagic N into the benthic compartment for a time longer than the characteristic time of phytoplankton dynamics (month-years vs. day-week). (C) 2003 Elsevier B.V. Alt rights reserved. [less ▲]

Detailed reference viewed: 59 (17 ULg)
Full Text
Peer Reviewed
See detailPromoter characterization of the mouse melanin-concentrating hormone receptor 1
Lakaye, Bernard ULg; Adamantidis, Antoine ULg; Coumans, Bernard ULg et al

in Biochimica et Biophysica Acta-Gene Structure and Expression (2004), 1678(1), 1-6

The gene encoding the mouse melanin-concentrating hormone receptor 1 was isolated and its structural organization and flanking regions were characterized. The 3' flanking region is marked by the presence ... [more ▼]

The gene encoding the mouse melanin-concentrating hormone receptor 1 was isolated and its structural organization and flanking regions were characterized. The 3' flanking region is marked by the presence of two polyadenylation signals but used with different frequencies. RNase protection and 5' rapid amplification of cDNA ends (RACE) identified multiple transcription initiation sites between -150 and -203 bp upstream of the ATG initiation codon. Functional analysis of deletion mutants reveals a cell independent transcriptional activity localized between nucleotide -305 and -589. The proximal 1.5 kb region does not possess consensus TATA or CAAT boxes but has several consensus sequences for regulatory elements including USF, GATA, AP1, AP4, MyoD, GKLF and Ikaros that could explain the broad expression of the receptor. [less ▲]

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailExperimental lifetime determination in neutral praseodymium (Pr I) and neodymium (Nd I)
Biémont, Emile ULg; Quinet, Pascal ULg; Svanberg, S. et al

in Journal of Physics B-Atomic Molecular and Optical Physics (2004), 37(7), 1381-1389

Radiative lifetime measurements have been performed, with a time-resolved laser-induced fluorescence technique, for 18 even-parity levels of Pr I and 15 odd-parity levels of Nd I. The new results are ... [more ▼]

Radiative lifetime measurements have been performed, with a time-resolved laser-induced fluorescence technique, for 18 even-parity levels of Pr I and 15 odd-parity levels of Nd I. The new results are compared with the few experimental data available in the literature and good agreement is found. However, fragmentary knowledge of the spectra prevents reliable calculation of branching fractions and calls for additional laboratory efforts in order to provide the astrophysicists with reliable transition probabilities needed for investigating the magnetic fields in chemically peculiar stars. [less ▲]

Detailed reference viewed: 43 (0 ULg)
Full Text
Peer Reviewed
See detailElectrochemical formation of polypyrrole nanowires
Jérôme, Christine ULg; Labaye, David; Jérôme, Robert ULg

in Synthetic Metals (2004), 142(1-3), 207-216

This paper aims at approaching the mechanism of formation of polypyrrole (PPy) nanowires by an all-electrochemical process, which consists in electrografting a poly(alkylacrylate) film onto the surface of ... [more ▼]

This paper aims at approaching the mechanism of formation of polypyrrole (PPy) nanowires by an all-electrochemical process, which consists in electrografting a poly(alkylacrylate) film onto the surface of a cathode, followed by the anodic electropolymerization of pyrrole (Py) at the surface of this organomodified electrode. The experimental conditions for the pyrrole oxidation, including solvent and concentrations of monomer and supporting electrolyte, have been changed with the purpose to tune the characteristic features of the polypyrrole nanowires. The polyacrylate template has also been modified, all other conditions being the same, in an effort to design the shape and size of the wires. The electrochemical behavior of the as-prepared PPy wires has been characterized. [less ▲]

Detailed reference viewed: 63 (2 ULg)
Full Text
Peer Reviewed
See detailNafion-layered silicate nanocomposite membrane for fuel cell application
Thomassin, Jean-Michel ULg; Pagnoulle, Christophe; Bizzari, Didier et al

in e-Polymers (2004), (18), 1-13

Direct methanol fuel cells (DMFCs) using a proton exchange membrane as electrolyte is an attractive option for electricity generation. The most widely used membrane in the DMFC system is based on a ... [more ▼]

Direct methanol fuel cells (DMFCs) using a proton exchange membrane as electrolyte is an attractive option for electricity generation. The most widely used membrane in the DMFC system is based on a perfluorinated polymer bearing sulfonic acid functions like Nafion®. The latter combines chemical, mechanical and thermal stability and high protonic conductivity but shows elevated methanol permeability. We propose the preparation of a novel type of hybrid membranes to tentatively solve this problem. This innovative material results from the homogeneous dispersion of a nano-scaled inorganic filler within Nafion. The filler consists of stacks of negatively charged alumino-silicate layers (Cloisite), with a positive counter-ion in the interlamellar space. The purpose of the addition of this filler is to decrease methanol diffusion through the polymer membrane without decreasing too much the ionic conductivity. [less ▲]

Detailed reference viewed: 103 (3 ULg)
Full Text
Peer Reviewed
See detailThe inhibition of metallo-beta-lactamase by thioxo-cephalosporin derivatives
Tsang, W. Y.; Dhanda, A.; Schofield, C. J. et al

in Bioorganic & Medicinal Chemistry Letters (2004), 14(7), 1737-1739

The 8-thioxocephalosporins are poor substrates for the B. cereus metallo beta-lactamase (k(cat)/K-m = 61.4 M-1 s(-1)) and act as weak competitive inhibitors (K-i similar to 700 muM). The hydrolysis ... [more ▼]

The 8-thioxocephalosporins are poor substrates for the B. cereus metallo beta-lactamase (k(cat)/K-m = 61.4 M-1 s(-1)) and act as weak competitive inhibitors (K-i similar to 700 muM). The hydrolysis product of thioxocephalosporin, a thioacid, also inhibits the enzyme competitively with a K-i = 96 muM, whereas the cyclic thioxo-piperazinedione, formed by intramolecular aminolysis of thioxo-cephalexin has a K-i of 29 muM. (C) 2004 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 168 (0 ULg)
Full Text
Peer Reviewed
See detailUp-regulation of vascular endothelial growth factor-A by active membrane-type 1 matrix metalloproteinase through activation of Src-tyrosine kinases
Sounni, Nor Eddine ULg; Roghi, C.; Chabottaux, Vincent et al

in Journal of Biological Chemistry (2004), 279(14), 13564-13574

Membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor ( VEGF) are two key molecules involved in pericellular proteolysis and cell proliferation during tumor growth and ... [more ▼]

Membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor ( VEGF) are two key molecules involved in pericellular proteolysis and cell proliferation during tumor growth and angiogenesis. Our previous data showed that MT1-MMP overexpression in human breast carcinoma MCF7 cells induced an up-regulation of VEGF expression. This effect was associated in vivo with accelerated tumor growth and angiogenesis. We now provide evidence that MT1-MMP overexpression specifically affected VEGF-A production and failed to influence that of other VEGF family members ( VEGF, B, C, D, or PlGF) or their receptors. The up-regulation of VEGF-A by MT1-MMP was related to an increased transcriptional activation rather than to a modification of mRNA stability. It was blocked by synthetic MMP inhibitors, TIMP2, but not TIMP-1 and abolished by a partial deletion of the catalytic domain or the cytoplasmic tail of MT1-MMP. Analysis of the signal transduction mechanisms demonstrated that MT1-MMP acts through a signaling pathway involving Src tyrosine kinases. Thus, our results provide new insight into the mechanisms of action of MT1-MMP during angiogenesis and suggest that the full enzymatic activity of MT1-MMP is required for a specific up-regulation of VEGF-A through an activation of Src tyrosine kinase pathways. [less ▲]

Detailed reference viewed: 27 (9 ULg)
Full Text
Peer Reviewed
See detailEvidence of an intramolecular interaction between the two domains of the BlaR1 penicillin receptor during the signal transduction
Hanique, Sophie; Colombo, Maria-Luigi; Goormaghtigh, Erik et al

in Journal of Biological Chemistry (2004), 279(14), 14264-14272

The BlaR1 protein is a penicillin-sensory transducer involved in the induction of the Bacillus licheniformis beta-lactamase. The amino-terminal domain of the protein exhibits four transmembrane segments ... [more ▼]

The BlaR1 protein is a penicillin-sensory transducer involved in the induction of the Bacillus licheniformis beta-lactamase. The amino-terminal domain of the protein exhibits four transmembrane segments (TM1-TM4) that form a four-alpha-helix bundle embedded in the plasma bilayer. The carboxyl-terminal domain of 250 amino acids (BlaR-CTD) fused at the carboxyl end of TM4 possesses the amino acid sequence signature of penicillin-binding proteins. This membrane topology suggests that BlaR-CTD and the BlaR-amino-terminal domain are responsible for signal reception and signal transduction, respectively. With the use of phage display experiments, we highlight herein an interaction between BlaR-CTD and the extracellular, 63-amino acid L2 loop connecting TM2 and TM3. This interaction does not occur in the presence of penicillin. This result suggests that binding of the antibiotic to BlaR1 might entail the release of the interaction between L2 and BlaR-CTD, causing a motion of the alpha-helix bundle and transfer of the information to the cytoplasm of the cell. In addition, fluorescence spectroscopy, CD, and Fourier transform IR spectroscopy experiments indicate that in contrast to the behavior of the corresponding Staphylococcus aureus protein, the beta-lactam antibiotic does not induce a drastic conformational change in B. licheniformis BlaR-CTD. [less ▲]

Detailed reference viewed: 23 (6 ULg)
Full Text
Peer Reviewed
See detailAménagement du territoire, urbanisme, patrimoine et questions diverses de droit administratif notarial
Pâques, Michel ULg; Delnoy, Michel ULg; Leprince, S. et al

in Chronique de Droit à l'Usage du Notariat (2004), XXXIX

Detailed reference viewed: 59 (13 ULg)
Full Text
Peer Reviewed
See detailEvolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates
Zecchin, E.; Mavropoulos, A.; Filippi, A. et al

in Developmental Biology (2004), 268(1), 174-184

We have characterized and mapped the zebrafish ptf1a gene, analyzed its embryonic expression, and studied its role in pancreas development. In situ hybridization experiments show th at from the 12-somite ... [more ▼]

We have characterized and mapped the zebrafish ptf1a gene, analyzed its embryonic expression, and studied its role in pancreas development. In situ hybridization experiments show th at from the 12-somite stage to 48 hpf, ptf1a is dynamically expressed in the spinal cord, hindbrain, cerebellum, retina, and pancreas of zebrafish embryos. Within the endoderm, ptf1a is initially expressed at 32 hpf in the ventral portion of the pdx1 expression domain; ptf1a is expressed in a subset of cells located on the left side of the embryo posteriorly to the liver primordium and anteriorly to the endocrine islet that arises from the posterodorsal pancreatic anlage. Then the ptf1a expression domain buds giving rise to the anteroventral pancreatic anlage that grows posteriorly to eventually engulf the endocrine islet. By 72 hpf, ptf1a continues to be expressed in the exocrine compartment derived from the anteroventral anlage. Morpholino-induced ptf1a loss of function suppresses the expression of the exocrine markers, while the endocrine markers in the islet are unaffected. In mind bomb (mib) mutants, in which delta-mediated notch signalling is defective [Dev. Cell 4 (2003) 67], ptf1a is normally expressed. In addition, the slow-muscle-omitted (smu) mutants that lack expression of endocrine markers because of a defective hedgehog signalling [Curr. Biol. 11(2001) 1358] exhibit normal levels of ptf1a. This indicates that hedgehog signaling plays a different genetic role in the specification of the anteroventral (mostly exocrine) and posterodorsal (endocrine) pancreatic anlagen. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailSpectroscopic anatomy of a meteor trail cross section with the European Southern Observatory Very Large Telescope
Jenniskens, Peter; Jehin, Emmanuel ULg; Cabanac, Remi A et al

in Meteoritics & Planetary Science (2004), 39

A meteor spectrum was recorded serendipitously at the European Southern Observatory (ESO) Very Large Telescope (VLT) during a long exposure in long-slit spectroscopic mode with FORS1. The -8 magnitude ... [more ▼]

A meteor spectrum was recorded serendipitously at the European Southern Observatory (ESO) Very Large Telescope (VLT) during a long exposure in long-slit spectroscopic mode with FORS1. The -8 magnitude fireball crossed the narrow 1 × 7 slit during the observation of a high z supernova in normal service mode operation on May 12, 2002. The spectrum covered the range of 637-1050 nm, where the meteor's air plasma emissions from N2, N, and O dominate. Carbon atom emission was not detected in the relatively unexplored wavelength range above 900 nm, but the observed upper limit was only 3 sigma less than expected from the dissociation of atmospheric CO2. The meteor trail was resolved along the slit, and the emission had a Gaussian distribution with a dimension of FWHM = 7.0 (±0.4) * sin(a) * H (km)/90 m, where a is the unknown angle between the orientation of the meteor path and slit and H the assumed altitude of the meteor in km. To our knowledge, this is the first observation of a spatially resolved spectrum across a meteor trail. Unlike model predictions, the plasma excitation temperature varied only from about 4,300 to 4,365 K across the trail, based on the ratio of atomic and molecular nitrogen emissions. Unfortunately, we conclude that this was because the meteor at 100 km altitude was out of focus. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailHD 108: The mystery deepens with XMM-Newton observations
Nazé, Yaël ULg; Rauw, Grégor ULg; Vreux, Jean-Marie ULg et al

in Astronomy and Astrophysics (2004), 417(2), 667-677

In 2001, using a large spectroscopic dataset from an extensive monitoring campaign, we discovered that the peculiar Of star HD 108 displayed extreme line variations. This strange behaviour could be ... [more ▼]

In 2001, using a large spectroscopic dataset from an extensive monitoring campaign, we discovered that the peculiar Of star HD 108 displayed extreme line variations. This strange behaviour could be attributed to a variety of models, and an investigation of the high energy properties of HD 108 was needed to test the predictions from these models. Our dedicated XMM-Newton observation of HD 108 shows that its spectrum is well represented by a two temperature thermal plasma model with kT(1) similar to 0.2 keV and kT(2) similar to 1.4 keV. In addition, we find that the star does not display any significant short-term changes during the XMM-Newton exposure. Compared to previous Einstein and ROSAT detections, it also appears that HD 108 does not present long-term flux variations either. While the line variations continue to modify HD 108's spectrum in the optical domain, the X-ray emission of the star appears thus surprisingly stable: no simple model is for the moment able to explain such an unexpected behaviour. Thanks to its high sensitivity, the XMM-Newton observatory has also enabled the serendipitous discovery of 57 new X-ray sources in the field of HD 108. Their properties are also discussed in this paper. [less ▲]

Detailed reference viewed: 28 (5 ULg)
Full Text
Peer Reviewed
See detailEfficacy of alphacalcidol and calcitriol in primary and corticosteroid-induced osteoporosis: a meta-analysis of their effects on bone mineral density and fracture rate
Richy, F.; Ethgen, Olivier ULg; Bruyère, Olivier ULg et al

in Osteoporosis International (2004), 15(4), 301-310

Vitamin D metabolites alphacalcidol and calcitriol (D-hormones) have been investigated for two decades, but few and conflicting results are available from high-quality randomized controlled trials. Our ... [more ▼]

Vitamin D metabolites alphacalcidol and calcitriol (D-hormones) have been investigated for two decades, but few and conflicting results are available from high-quality randomized controlled trials. Our objectives were to provide an evidence-based update quantitatively summarizing their efficacy on bone mineral density (BMD) and fracture rate. We performed a systematic research of any randomized controlled trial containing relevant data, peer review, data extraction and quality scoring blinded for authors and data sources, and comprehensive meta-analyses of the relevant data. Inclusion criteria were: randomized controlled study, calcitriol or alphacalcidol, BMD or fractures in healthy/osteopenic/osteoporotic patients exposed or not to corticosteroids (CS). Analyses were performed in a conservative fashion using professional dedicated softwares and stratified by outcome, target patients, study quality, and control-group type. Results were expressed as effect size (ES) for bone loss or relative risk (RR) for fracture while allocated to D-hormones vs control. Publication bias and robustness were investigated. Of the trials that were retrieved and subsequently reviewed, 17 papers fitted the inclusion criteria and were assessed. Quality scores ranged from 20 to 100%, the mean (standard deviation) being 72 (22)%. Calcitriol and alphacalcidol were found to have the same efficacy on all outcomes at p>0.13. We globally assessed D-hormones effects in preventing bone loss in patients not exposed to CS, and found positive effect: ES=0.39 (p<0.001). For lumbar spine, this particular effect was 0.43 (p<0.001). D-hormones significantly reduced the overall fracture rates: RR=0.52 (0.46; 0.59) and both vertebral and non-vertebral fractures: RR=0.53 (0.47; 0.60) and RR=0.34 (0.16; 0.71), respectively. No statistical difference in response was observed between results from studies on healthy and osteoporotic patients or depending on the fact that controls were allowed to calcium supplementation. Treatment with D-hormones was evaluated for maintaining spinal bone mass in five trials of patients with CS-induced osteoporosis, and provided ES=0.43 at p<0.001. Only two studies specifically addressed the effects of calcitriol on spinal fracture rate. None of them provided significant results, and the global RR did not reach the significance level as well: RR=0.33 (0.07; 1.51). Our data demonstrated efficacy for DH on bone loss and fracture prevention in patients not exposed to CS and on bone loss in patients exposed to CS, in the light of the most reliable scientific evidence. Their efficacy in reducing the number of fractures in patients exposed to CS remains to be determined. [less ▲]

Detailed reference viewed: 42 (7 ULg)
Full Text
Peer Reviewed
See detailIntermittent treatment of knee osteoarthritis with oral chondroitin sulfate: a one-year, randomized, double-blind, multicenter study versus placebo
Uebelhart, D.; Malaise, Michel ULg; Marcolongo, R. et al

in Osteoarthritis and Cartilage (2004), 12(4), 269-276

Objective: To investigate the efficacy and tolerability of a 3-month duration, twice a-year, intermittent treatment with oral chondroitin sulfate (CS) in knee osteoarthritis (OA) patients. Design: A total ... [more ▼]

Objective: To investigate the efficacy and tolerability of a 3-month duration, twice a-year, intermittent treatment with oral chondroitin sulfate (CS) in knee osteoarthritis (OA) patients. Design: A total of 120 patients with symptomatic knee OA were randomized into two groups receiving either 800 mg CS or placebo (PBO) per day for two periods of 3 months during 1 year. Primary efficacy outcome was Lequesne's algo-functional index (AFI); secondary outcome parameters included VAS, walking time, global judgment, and paracetamol consumption. Radiological progression was assessed by automatic measurement of medial femoro-tibial joint space width on weight-bearing X-rays of both knees. Clinical and biological tolerability was assessed. Results: One hundred and ten of 120 patients were included in the ITT analysis. AFI decreased significantly by 36% in the CS group after 1 year as compared to 23% in the PBO group. Similar results were found for the secondary outcomes parameters. Radiological progression at month 12 showed significantly decreased joint space width in the PBO group with no change in the CS group. Tolerability was good with only minor adverse events identically observed in both groups. Conclusion: This study provides evidences that oral CS decreased pain and improved knee function. The 3-month intermittent administration of 800 mg/day of oral CS twice a year does support the prolonged effect known with symptom-modifying agents for OA. The inhibitory effect of CS on the radiological progression of the medial femoro-tibial joint space narrowing could suggest further evidence of its structure-modifying properties in knee OA. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Full Text
Peer Reviewed
See detailSpontaneous tracheal rupture: a case report
Rousie, Céline ULg; Van Damme, Hendrik ULg; Radermecker, Maurice ULg et al

in Acta Chirurgica Belgica (2004), 104(2), 204-208

We report the case of a spontaneous posterior tracheal wall rupture following a cough. A 67-year-old woman with a history of longstanding treatment with corticosteroids (8 years) for Giant Cell Arteritis ... [more ▼]

We report the case of a spontaneous posterior tracheal wall rupture following a cough. A 67-year-old woman with a history of longstanding treatment with corticosteroids (8 years) for Giant Cell Arteritis had general anesthesia for cataract removal. Surgery and anesthesia were uneventful. In the recovery room, the patient coughed and soon after developed subcutaneous emphysema of the neck. Chest radiography confirmed the clinical diagnosis of marked subcutaneous emphysema and showed huge pneumomediastinum and minor right pneumothorax. A thoracic CT scan revealed a large laceration of the posterior tracheal wall (a 4 cm longitudinal tear), extending from the middle of the trachea to the level of the carina. Surgical repair consisted in closure of the dilaceration using an autologous pericardial patch. It seems reasonable to suspect the facilitating role of connective tissue fragility due to chronic corticosteroid administration in the development of this tracheal rupture following cough. Tracheal rupture is a potentially lethal injury, which can be repaired successfully if the diagnosis is made early. Risk factors, diagnosis and principles of treatment of this lesion are discussed. [less ▲]

Detailed reference viewed: 78 (2 ULg)
Full Text
Peer Reviewed
See detailSuperinfection prevents recombination of the alphaherpesvirus bovine herpesvirus 1
Meurens, F.; Schynts, F.; Keil, G. M. et al

in Journal of Virology (2004), 78(8), 3872-3879

Homologous recombination between strains of the same alphaherpesvirus species occurs frequently both in vitro and in vivo. This process has been described between strains of herpes simplex virus type 1 ... [more ▼]

Homologous recombination between strains of the same alphaherpesvirus species occurs frequently both in vitro and in vivo. This process has been described between strains of herpes simplex virus type 1, herpes simplex virus type 2, pseudorabies virus, feline herpesvirus 1, varicella-zoster virus, and bovine herpesvirus 1 (BoHV-1). In vivo, the rise of recombinant viruses can be modulated by different factors, such as the dose of the inoculated viruses, the distance between inoculation sites, the time interval between inoculation of the first and the second virus, and the genes in which the mutations are located. The effect of the time interval between infections with two distinguishable BoHV-1 on recombination was studied in three ways: (i) recombination at the level of progeny viruses, (ii) interference induced by the first virus infection on beta-gallactosidase gene expression of a superinfecting virus, and (iii) recombination at the level of concatemeric DNA. A time interval of 2 to 8 h between two successive infections allows the establishment of a barrier, which reduces or prevents any successful superinfection needed to generate recombinant viruses. The dramatic effect of the time interval on the rise of recombinant viruses is particularly important for the risk assessment of recombination between glycoprotein E-negative marker vaccine and field strains that could threaten BoHV-1 control and eradication programs. [less ▲]

Detailed reference viewed: 21 (2 ULg)
Full Text
Peer Reviewed
See detailPrise en charge des exacerbations d'asthme
Mommens, Véronique; Louis, Renaud ULg; D'Orio, Vincenzo ULg

in Revue Médicale de Liège (2004), 59(4), 209-14

Asthma exacerbations constitute a common medical emergency, which in the past has been shown to be poorly managed. Although its management has recently improved, it remains suboptimal. For instance, acute ... [more ▼]

Asthma exacerbations constitute a common medical emergency, which in the past has been shown to be poorly managed. Although its management has recently improved, it remains suboptimal. For instance, acute life-threatening asthma is still responsible for 2000 death each year in France. More than two thirds of deaths should be avoided by a prompt and adjusted treatment. Initial assessment of the severity of the exacerbation is fundamental. The clinical story, physical examination and objective measurement of airflow obstruction by peak flow meter should permit to quickly establish an adapted treatment. The aim of this paper is, once general considerations about clinical approach and currently used treatments have been made, to propose a home and a hospital-based management of the asthma exacerbations. [less ▲]

Detailed reference viewed: 72 (3 ULg)
Full Text
Peer Reviewed
See detailNeurophysiological tests and neuroimaging procedures in non-acute headache: guidelines and recommendations
Sandrini, G.; Friberg, L.; Janig, W. et al

in European Journal of Neurology (2004), 11(4), 217-224

The use of instrumental examinations in headache patients varies widely. In order to evaluate their usefulness, the most common instrumental procedures were evaluated, on the basis of evidence from the ... [more ▼]

The use of instrumental examinations in headache patients varies widely. In order to evaluate their usefulness, the most common instrumental procedures were evaluated, on the basis of evidence from the literature, by an EFNS Task Force (TF) on neurophysiological tests and imaging procedures in non-acute headache patients. The conclusions of the TF regarding each technique are expressed in the following guidelines for clinical use. 1 Interictal electroencephalography (EEG) is not routinely indicated in the diagnostic evaluation of headache patients. Interictal EEG is. however, indicated if the clinical history suggests a possible diagnosis of epilepsy (differential diagnosis). Ictal EEG could be useful in certain patients suffering from hemiplegic and basilar migraine. 2 Recording of evoked potentials is not recommended for the diagnosis of headache disorders. 3 There is no evidence to justify the recommendation of autonomic tests for the routine clinical examination of headache patients. 4 Manual palpation of pericranial muscles, with standardized palpation pressure, can be recommended for subdividing patient groups but not for diagnosis. Pressure algometry and electromyography (EMG) cannot be recommended as clinical diagnostic tests. 5 In adult and paediatric patients with migraine, with no recent change in attack pattern, no history of seizures, and no other Focal neurological signs or symptoms, the routine use of neuroimaging is not warranted. In patients with atypical headache patterns, a history Of seizures and/or focal neurological signs or symptoms, magnetic resonance imaging (MRI) may be indicated. 6 If attacks can be fully accounted for by the standard headache classification [International Headache Society (IHS)], a positron emission tomography (PET) or single-photon emission computerized tomography (SPECT) and scan will generally be of no further diagnostic value. 7 Nuclear medicine examinations of the cerebral circulation and metabolism can be carried out in Subgroups of headache patients for diagnosis and evaluation of complications, when patients experience unusually severe attacks, or when the quality or severity of attacks has changed. 8 Transcranial Doppler examination is not helpful in headache diagnosis. Although many of the examinations described are of little or no value in the clinical setting, most of the tools have a vast potential for further exploring the pathophysiology of headaches and the effects of pharmacological treatment. [less ▲]

Detailed reference viewed: 58 (0 ULg)
Full Text
Peer Reviewed
See detailRecommendations for the use of new methods to assess the efficacy of disease-modifying drugs in the treatment of osteoarthritis
Abadie, Eric ULg; Ethgen, Dominique ULg; Avouac, Bernard ULg et al

in Osteoarthritis and Cartilage (2004), 12(4), 263-268

Background: Recent innovations in the pharmaceutical drug discovery environment have generated new chemical entities with the potential to become disease modifying drugs for osteoarthritis (DMOAD's ... [more ▼]

Background: Recent innovations in the pharmaceutical drug discovery environment have generated new chemical entities with the potential to become disease modifying drugs for osteoarthritis (DMOAD's). Regulatory agencies acknowledge that such compounds may be granted a DMOAD indication, providing they demonstrate that they can slow down disease progression; progression would be calibrated by a surrogate for structural change, by measuring joint space narrowing (JSN) on plain X-rays with the caveat that this delayed JSN translate into a clinical benefit for the patient. Recently, new technology has been developed to detect a structural change of the OA joint earlier than conventional X-rays. Objective: The Group for the Respect of Ethics and Excellence in Science (GREES) organized a working party to assess whether these new technologies may be used as surrogates to plain x-rays for assessment of DMOADs. Methods: GREES includes academic scientists, members of regulatory authorities and representatives from the pharmaceutical industry. After an extensive search of the international literature, from 1980 to 2002, two experts meetings were organized to prepare a resource document for regulatory authorities. This document includes recommendations for a possible update of guidelines for the registration of new chemical entities in osteoarthritis. Results: Magnetic resonance imaging (MRI) is now used to measure parameters of cartilage morphology and integrity in OA patients. While some data are encouraging, correlation between short-term changes in cartilage structure observed with MRI and long-term radiographic or clinical changes are needed. Hence, the GREES suggests that MRI maybe used as an outcome in phase II studies, but that further data is needed before accepting MRI as a primary end-point in phase III clinical trials. Biochemical markers of bone and cartilage remodelling are being tested to predict OA and measure disease progression. Recently published data are promising but validation as surrogate end-points for OA disease progression requires additional study. The GREES suggests that biochemical markers remain limited to 'proof of concept' studies or as secondary end-points in phase II and III clinical trials. However, the GREES emphasizes the importance of acquiring additional information on biochemical markers in order to help better understand the mode of action of drugs to be used in OA. Regulatory agencies consider that evidence of improvement in clinical outcomes is critical for approval of DMOAD. Time to total joint replacement surgery is probably the most relevant clinical end-point for the evaluation of efficacy of a DMOAD. However, at this time, time to surgery can not be used in clinical trials because of bias by non disease-related factors like patient willingness for surgery or economic factors. At this stage, it appears that DMOAD should demonstrate a significant difference compared to placebo. Benefit should be measured by 3 co-primary end-points: JSN, pain and function. Secondary end-points should include the percentage of patients who are 'responder' (or 'failure'). The definition of a 'failure' patient would be someone with progression of JSN>0.5 mm over a period of 2-3 years or who has a significant worsening in pain and/or function, based on validated cut-off values. The definition of the clinically relevant cut-off points for pain and function must be based on data evaluating the natural history of the disease (epidemiological cohorts or placebo groups from long-term studies). These cut-offs points should reflect a high propensity, for an individual patient, to later require joint replacement. Conclusion: GREES has outlined a set of guidelines for the development of a DMOAD for OA. Although these guidelines are subject to change as new information becomes available, the information above is based on the present knowledge in the field with the addition of expert opinion. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 122 (13 ULg)
Full Text
Peer Reviewed
See detailHeadache with focal neurological signs or symptoms: a complicated differential diagnosis
Schoenen, Jean ULg; Sandor, P. S.

in Lancet Neurology (2004), 3(4), 237-245

Headache syndromes can be associated with focal neurological symptoms or signs. Good knowledge of primary headaches, a detailed history and a thorough clinical examination are prerequisites for their ... [more ▼]

Headache syndromes can be associated with focal neurological symptoms or signs. Good knowledge of primary headaches, a detailed history and a thorough clinical examination are prerequisites for their differential diagnosis. The neurological symptoms produced by the migraine aura are the most characteristic and recognisable. However, structural lesions, such as vascular malformations, can produce similar symptoms to migraine with aura, which highlights that paraclinical investigations are necessary in most patients with headache and focal neurological symptoms. In this review, we provide an overview of the differential diagnosis of the most common headache disorders with focal neurological symptoms or signs to refresh the practising neurologist's differential diagnostic knowledge for the clinical situation and to aid the teaching of neurology residents. [less ▲]

Detailed reference viewed: 69 (2 ULg)
Full Text
Peer Reviewed
See detailU-Pb zircon calendar for Namaquan (Grenville) crustal events in the granulite-facies terrane of the O'okiep Copper District of South Africa
Clifford, Tom N.; Barton, Erika S; Stern, Richard A. et al

in Journal of Petrology (2004), 45(4), 669-691

The O'okiep Copper District is underlain by voluminous 1035-1210 Ma granite gneiss and granite with remnants of metamorphosed supracrustal rocks. This assemblage was intruded by the 1030 Ma copper-bearing ... [more ▼]

The O'okiep Copper District is underlain by voluminous 1035-1210 Ma granite gneiss and granite with remnants of metamorphosed supracrustal rocks. This assemblage was intruded by the 1030 Ma copper-bearing Koperberg Suite that includes jotunite, anorthosite, biotite diorite and hypersthene-bearing rocks ranging from leuconorite to hypersthenite. New sensitive high-resolution ion microprobe age data demonstrate the presence of 1700-2000 Ma zircon as xenocrysts in all of the intrusive rocks, and as detrital zircon in the metasediments of the Khurisberg Subgroup. These data are consistent with published Sm-Nd model ages of c. 1700 Ma (T-CHUR) and c. 2000 Ma (T-DM) of many of the intrusives that support a major crust-forming event in Eburnian (Hudsonian) times. In addition, U-Th-Pb analyses of zircons from all major rock units define two tectono-magmatic episodes of the Namaquan Orogeny: (1) the O'okiepian Episode (1180-1210 Ma), represented by regional granite plutonism, notably the Nababeep and Modderfontein Granite Gneisses and the Concordia and Kweekfontein Granites that accompanied and outlasted (e.g. Kweekfontein Granite) regional tectonism [F-2(D-2)] and granulite-facies metamorphism (M-2); (2) the Klondikean Episode (1020-1040 Ma), which includes the intrusion of the porphyritic Rietberg Granite and of the Koperberg Suite that are devoid of regional planar or linear fabrics. Klondikean tectonism (D-3) is reflected by major east-west-trending open folds [F-3(D-3a)], and by localized east-west-trending near-vertical ductile folds ['steep structures'; F-4(D-3b)] whose formation was broadly coeval with the intrusion of the Koperberg Suite. A regional, largely thermal, amphibolite- to granulite-facies metamorphism (M-3) accompanied D-3. This study demonstrates, inter alia, that the complete spectrum of rock-types of the Koperberg Suite, together with the Rietberg Granite, was intruded in a short time-interval (<10 Myr) at c. 1030 Ma, and that there were lengthy periods of about 150 Myr of tectonic quiescence within the Namaquan Orogeny: (1) between the O'okiepian and Klondikean Episodes; (2) from the end of the latter to the formal end of Namaquan Orogenesis 800-850 Ma ago. [less ▲]

Detailed reference viewed: 57 (5 ULg)
Full Text
Peer Reviewed
See detailLC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam
Ouattara, Badiore; Angenot, Luc ULg; Guissou, Pierre et al

in Phytochemistry (2004), 65(8), 1145-1151

Gradient HPLC coupled to DAD/UV, MS/MS and NMR has been applied to the rapid structure determination of three new isomeric divanilloylquinic acids from Fagara zanthoxyloides collected in Burkina Faso: 3,4 ... [more ▼]

Gradient HPLC coupled to DAD/UV, MS/MS and NMR has been applied to the rapid structure determination of three new isomeric divanilloylquinic acids from Fagara zanthoxyloides collected in Burkina Faso: 3,4-O-divanilloylquinic acid, 3,5-O-divanilloylquinic acid and 4,5-O-divanilloylquinic acid. Furthermore these new compounds named burkinabins A-C could play a useful role in sickle cell disease, as the active agents of Fagara zanthoxyloides are said to be unidentified aromatic compounds with carboxylic acid grouping (Adesanya, S.A., Sofowora, A., 1983. Biological standardisation of Zanthoxylum roots for antisickling activity. Planta Med. 48, 27-33). (C) 2004 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 136 (31 ULg)
Full Text
Peer Reviewed
See detailCrural or pedal artery revascularisation for limb salvage: is it justified
Van Damme, Hendrik ULg

in Acta Chirurgica Belgica (2004), 104(2), 148-157

With the ageing of population, the incidence of limb-threatening ischemia increases. In chronic critical limb ischemia, peripheral arterial occlusive disease almost always involves infrainguinal and ... [more ▼]

With the ageing of population, the incidence of limb-threatening ischemia increases. In chronic critical limb ischemia, peripheral arterial occlusive disease almost always involves infrainguinal and infragenicular vessels. Fortunately, recent advances in vascular surgery made arterial reconstruction of crural and pedal vessels possible. Should crural or pedal bypass surgery be offered to these frail, polyvascular patients, or is primary amputation a preferable treatment option in case of advanced limb-threatening ischemia? In order to answer this controversial question, the author analysed recent literature data on the feasibility and durability of infrapopliteal bypasses. The quality of life was also considered as an outcome measure. Finally, the cost-effectiveness of both treatment modalities (limb-saving distal bypass versus primary amputation) was assessed. [less ▲]

Detailed reference viewed: 42 (0 ULg)
Full Text
Peer Reviewed
See detailLe syndrome de Boerhaave
Honore, Pierre ULg; De Roover, Arnaud ULg; Detry, Olivier ULg et al

in Revue Médicale de Liège (2004), 59(4), 203-4

Spontaneous rupture of the oesophagus, so called Boerhaave's syndrome, still remains a surgical emergency. Early diagnosis governs the appropriate repair and the vial prognosis.

Detailed reference viewed: 93 (6 ULg)
Full Text
Peer Reviewed
See detailLiving related liver transplantation in adults: First year experience at the University of Liege
Detry, Olivier ULg; De Roover, Arnaud ULg; Delwaide, Jean ULg et al

in Acta Chirurgica Belgica (2004), 104(2, MAR-APR), 166-171

Living related liver transplantation (LRLT) in adult recipients has been recently developed to overcome the organ donor shortage, but LRLT leaves the healthy donors at risk of serious post-operative ... [more ▼]

Living related liver transplantation (LRLT) in adult recipients has been recently developed to overcome the organ donor shortage, but LRLT leaves the healthy donors at risk of serious post-operative complications, or even death. The aim of this paper is to report the prospective evaluation of the initial experience of adult LRLT at the University of Liege. From March 2002 till March 2003, in a consecutive series of 35 adult liver transplantations, five recipients (mean age : 51 years) underwent LRLT, including one retransplantation. Indications for transplantation were autoimmune hepatitis, hepatitis B virus related cirrhosis with hepatocarcinoma (two cases), hepatitis C virus related cirrhosis with hepatocarcinoma, and ischemic intrahepatic bile duct necrosis 10 years after primary liver transplantation. Mean age of the donors was 34 years (range : 21-53 years). All donation cases were intra familial at first degree. The right lobe was used as a graft in four cases and the left lobe in one case. All right lobe donors developed transient hyperbilirubinemia and hypocoagulation for 4 to 6 days. No severe complication (transfusion, bile duct fistula, reintervention, rehospitalization) nor significant long-term sequelae were observed in the donors. In the recipients, graft function was immediate, and there was no small-for-size syndrome. One recipient developed biliary fistula treated by reoperation. One recipient died from invasive aspergillosis 11 days after the procedure. The four other recipients were alive without recurrence of the disease at follow-up. This report confirmed that LRLT may be a valuable alternative to cadaveric liver transplantation in the era of organ donor shortage. However, even if there was no severe complication for the donors in our preliminary experience, LRLT puts healthy living donors at risk of significant morbidity and even death. [less ▲]

Detailed reference viewed: 51 (15 ULg)
Full Text
Peer Reviewed
See detailThyrotoxic adenoma followed by atypical hyperthyroidism due to struma ovarii: clinical and genetic studies
Ciccarelli, A.; Valdes Socin, Hernan Gonzalo ULg; Parma, J. et al

in European Journal of Endocrinology (2004), 150(4), 431-437

OBJECTIVE: Atypical forms of hyperthyroidism represent a diagnostic challenge for clinicians. Struma ovarii is an ovarian teratoma and constitutes a rare cause of ectopic thyroidal hormonal production. We ... [more ▼]

OBJECTIVE: Atypical forms of hyperthyroidism represent a diagnostic challenge for clinicians. Struma ovarii is an ovarian teratoma and constitutes a rare cause of ectopic thyroidal hormonal production. We describe a case of struma ovarii that combined two different sources of hyperthyroidism in the same patient and report genetic studies in order to contribute a better understanding of the autonomy and tumorigenesis of the struma ovarii. CASE REPORT: A 73-year-old nulliparous woman presented a thyroid toxic adenoma that was successfully treated with 10 mCi radioiodine. Unexpectedly, a new onset of hyperthyroidism prompted us to look for a second etiology. A whole-body scan with (123)I detected a pelvic hyperfixation suggesting struma ovarii, and a thyroid differentiated left ovarian teratoma 3 cm in size was surgically removed. We screened for mutations of thyroid-stimulating hormone receptor and Gs-alpha protein genes, as these mutations are common in thyroid adenomas. We did not identify any mutations. Androgen receptor study demonstrated a monoclonal status. Comparative genomic hybridization did not reveal any chromosomal abnormality. However, loss of heterozygosity analysis showed several structural abnormalities, compared with the majority of benign ovarian teratomas, which show a normal karyotype. CONCLUSIONS: This is the first well-documented report of thyrotoxic struma ovarii revealed after treatment of a single thyroid toxic adenoma. We have shown in this case that struma ovarii originates from a single germ cell, and, albeit benign, this tumor presents several chromosomal abnormalities. Struma ovarii-induced hyperthyroidism is likely to be mediated by mechanisms different from those of the classical thyroid toxic adenoma. [less ▲]

Detailed reference viewed: 44 (3 ULg)
Full Text
Peer Reviewed
See detailL'angor
Legrand, Victor ULg

in Revue Médicale de Liège (2004), 59(4), 186-9

The medical history is the cornerstone of the diagnosis of chest pain. In presence of angina like symptoms, the diagnosis of coronary artery disease must be assertained by rest and exercise ECG ... [more ▼]

The medical history is the cornerstone of the diagnosis of chest pain. In presence of angina like symptoms, the diagnosis of coronary artery disease must be assertained by rest and exercise ECG, eventually coupled with a stress echo or scintigraphy. Coronary angiography remains the ultimate diagnostic procedure despite recent advances in CT scan or MRI technologies. Beside symptomatic treatment aiming to reduce metabolic demand by medication or to improve revascularisation by surgery or percutaneous intervention, the actual therapeutic approach involves therapies aiming to stop atherosclerosis such as strict correction of coronary risk factors, use of statines, ACE inhibitors and antiplatelet agents. [less ▲]

Detailed reference viewed: 27 (1 ULg)
Full Text
See detailTraitement d’une obésité associée à une hypercholestérolémie chez une chienne.
Jeusette, Isabelle; Peeters, Dominique ULg; Diez, Marianne ULg

in Le nouveau praticien vétérinaire (2004)

Cas clinique

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailJe ne suis pas un super héros
Dejasse, Erwin ULg

in 9e Art : Les Cahiers du Musée de la Bande Dessinée (2004), 10

Detailed reference viewed: 36 (3 ULg)
Full Text
Peer Reviewed
See detailDyspnee paroxystique chez le sujet sain: le pneumothorax spontane
Duysinx, Bernard ULg; Nguyen, Delphine; Corhay, Jean-Louis ULg et al

in Revue Médicale de Liège (2004), 59(4), 205-8

In a healthy individual, a dyspnoea of abrupt onset raises the suspicion of a spontaneous pneumothorax. The clinical examination and standard chest X-ray usually permit the correct diagnosis. The risk of ... [more ▼]

In a healthy individual, a dyspnoea of abrupt onset raises the suspicion of a spontaneous pneumothorax. The clinical examination and standard chest X-ray usually permit the correct diagnosis. The risk of reccurence can be assessed by the clinical history and examination. The functional consequences (which can be lethal) and the risk of recurrence will guide the short- and long-term therapy. [less ▲]

Detailed reference viewed: 165 (3 ULg)
Full Text
Peer Reviewed
See detailLe General et le Physicien. Modalites de rupture des anevrysmes de l'aorte abdominale : du paroxysme a la crise differee
Limet, Raymond ULg

in Revue Médicale de Liège (2004), 59(4), 201-2

Abdominal aortic rupture is sometimes paroxystic. More often, the rupture process takes hours, even days. Surgical efficiency is dependant on an early diagnosis.

Detailed reference viewed: 8 (2 ULg)
Full Text
Peer Reviewed
See detailMatrix metalloproteinases and tissue inhibitors of matrix metalloproteinases mRNA transcripts in the bronchial secretions of asthmatics
Cataldo, Didier ULg; Guéders, Maud ULg; Munaut, Carine ULg et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (2004), 84(4), 418-424

Asthma is a chronic inflammatory disease characterized by profound extracellular matrix changes referred to as bronchial remodelling. In this study, we evaluated matrix metalloproteinases (MMPs) and ... [more ▼]

Asthma is a chronic inflammatory disease characterized by profound extracellular matrix changes referred to as bronchial remodelling. In this study, we evaluated matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) mRNA expression in bronchial secretions of asthmatics and correlated MMPs modulations with the lung function as a reflection of the bronchial extracellular matrix remodelling. Quantitative RT-PCR was performed on cell pellets obtained from induced sputum in order to detect the mRNAs for MMP-1, -2, -3, -8, -9, -12, -13 TIMP-1, -2, while semiquantitative RT-PCR was performed to assess the expression of MMP-7, monocyte chemoattractant protein-1 (MCP-1) and transforming growth factor-beta(1) (TGF-beta(1)). The mRNA transcripts for MMP-1, TIMP-1 and monocyte chemoattractant protein-1 (MCP-1) were increased in cell pellets of induced sputum from asthmatics when compared to controls (P<0.05), and the intensity of MMP-1 mRNA expression inversely correlated with the FEV(1) in asthmatics (r=-0.49, P<0.05). The MMP-1 mRNA/TIMP-1 mRNA ratio correlated with the levels of MCP-1 mRNA in asthmatics (r=0.47, P<0.05). There were no differences between the groups with respect to mRNA coding for MMP-2, -3, -7, -8, -9, -12, -13, -14, TIMP-2 and TGF-beta(1). We conclude that cells contained in the bronchial secretions from asthmatics express higher amounts of mRNA for MMP-1 and TIMP-1, perhaps related to an increased expression of MCP-1, which might contribute to the extracellular matrix changes observed during airway remodelling. [less ▲]

Detailed reference viewed: 38 (7 ULg)
Full Text
Peer Reviewed
See detailA propos du rêve et son contenant
Dracoulis, Hélène; Rinné, Caroline; Gauthier, Jean-Marie ULg

in Revue française de psychiatrie et de psychologie médicale (2004), 7(75),

Detailed reference viewed: 52 (7 ULg)
Full Text
Peer Reviewed
See detailStrong interaction and E2 effect in even-A antiprotonic Te atoms
Klos, B.; Wycech, S.; Trzcinska, A. et al

in Physical Review C (2004), 69(4), 04431111

The x-ray cascade from antiprotonic atoms was studied for Te-122, Te-124, Te-126, Te-128, and Te-130. Widths and shifts due to the strong interaction were deduced for several levels. The E2 nuclear ... [more ▼]

The x-ray cascade from antiprotonic atoms was studied for Te-122, Te-124, Te-126, Te-128, and Te-130. Widths and shifts due to the strong interaction were deduced for several levels. The E2 nuclear resonance effect was observed in all investigated nuclei. In Te-130 the E2 resonance allowed to determine level widths and shifts of the LS-split deeply bound (n,l)=(6,5) state, otherwise unobservable. The measured level widths and shifts, corrected for the E2-resonance effect, were used to investigate the nucleon density in the nuclear periphery. The deduced neutron distributions are compared with results of the previously introduced radiochemical method and with Hartree-Fock-Bogoliubov model calculations. [less ▲]

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailThe novel somatostatin analog SOM230 is a potent inhibitor of hormone release by growth hormone- and prolactin-secreting pituitary adenomas in vitro.
Hofland, Leo J; van der Hoek, Joost; van Koetsveld, Peter M et al

in Journal of Clinical Endocrinology and Metabolism (2004), 89(4), 1577-1585

To determine the inhibitory profile of the novel somatostatin (SRIF) analog SOM230 with broad SRIF receptor binding, we compared the in vitro effects of SOM230, octreotide (OCT), and SRIF-14 on hormone ... [more ▼]

To determine the inhibitory profile of the novel somatostatin (SRIF) analog SOM230 with broad SRIF receptor binding, we compared the in vitro effects of SOM230, octreotide (OCT), and SRIF-14 on hormone release by cultures of different types of secreting pituitary adenomas. OCT (10 nM) significantly inhibited GH release in seven of nine GH-secreting pituitary adenoma cultures (range, -26 to -73%), SOM230 (10 nM) in eight of nine cultures (range, -22 to -68%), and SRIF-14 (10 nM) in six of six cultures (range, -30 to -75%). The sst analysis showed predominant but variable levels of somatostatin receptor (sst)(2) and sst(5) mRNA expression. In one culture completely resistant to OCT, SOM230 and SRIF-14 significantly inhibited GH release in a dose-dependent manner with an IC(50) value in the low nanomolar range. In the other cultures, SOM230 showed a lower potency of GH release inhibition (IC(50), 0.5 nM), compared with OCT (IC(50), 0.02 nM) and SRIF-14 (IC(50), 0.02 nM). A positive correlation was found between sst(2) but not sst(5) mRNA levels in the adenoma cells and the inhibitory potency of OCT on GH release in vivo and in vitro, and the effects of SOM230 and SRIF-14 in vitro. In three prolactinoma cultures, 10 nM OCT weakly inhibited prolactin (PRL) release in only one (-28%), whereas 10 nM SOM230 significantly inhibited PRL release in three of three cultures (-23, -51, and -64.0%). The inhibition of PRL release by SOM230 was related to the expression level of sst(5) but not sst(2) mRNA. Several conclusions were reached. First, SOM230 has a broad profile of inhibition of tumoral pituitary hormone release in the low nanomolar range, probably mediated via both sst(2) and sst(5) receptors. The higher number of responders of GH-secreting pituitary adenoma cultures to SOM230, compared with OCT, suggest that SOM230 has the potency to increase the number of acromegalic patients which can be biochemically controlled. Second, compared with OCT, SOM230 is more potent in inhibiting PRL release by mixed GH/PRL-secreting adenoma and prolactinoma cells. [less ▲]

Detailed reference viewed: 63 (3 ULg)
Full Text
Peer Reviewed
See detailClimate change impacts on groundwater resources: modelled deficits in a chalky aquifer, Geer basin, Belgium
Brouyère, Serge ULg; Carabin, Guy; Dassargues, Alain ULg

in Hydrogeology Journal (2004), 12(2), 123-134

An integrated hydrological model (MOHISE) was developed in order to study the impact of climate change on the hydrological cycle in representative water basins in Belgium. This model considers most ... [more ▼]

An integrated hydrological model (MOHISE) was developed in order to study the impact of climate change on the hydrological cycle in representative water basins in Belgium. This model considers most hydrological processes in a physically consistent way, more particularly groundwater flows which are modelled using a spatially distributed, finite-element approach. Thanks to this accurate numerical tool, after detailed calibration and validation, quantitative interpretations can be drawn from the groundwater model results. Considering IPCC climate change scenarios, the integrated approach was applied to evaluate the impact of climate change on the water cycle in the Geer basin in Belgium. The groundwater model is described in detail, and results are discussed in terms of climate change impact on the evolution of groundwater levels and groundwater reserves. From the modelling application on the Geer basin, it appears that, on a pluriannual basis, most tested scenarios predict a decrease in groundwater levels and reserves in relation to variations in climatic conditions. However, for this aquifer, the tested scenarios show no enhancement of the seasonal changes in groundwater levels. [less ▲]

Detailed reference viewed: 128 (28 ULg)
Full Text
Peer Reviewed
See detailMonstrueuse baleine
Dejasse, Erwin ULg

in 9e Art : Les Cahiers du Musée de la Bande Dessinée (2004), 10

Detailed reference viewed: 29 (0 ULg)
Full Text
Peer Reviewed
See detailPressure pain thresholds of tender point sites in patients with fibromyalgia and in healthy controls
Maquet, Didier ULg; Croisier, Jean-Louis ULg; Demoulin, Christophe ULg et al

in European Journal of Pain (London, England) (2004), 8(2), 111-117

Pressure pain threshold (PPT) is defined as the minimum force applied which induces pain. This measure has proven to be commonly useful in evaluating tenderness symptom. Our aim was to study the intra ... [more ▼]

Pressure pain threshold (PPT) is defined as the minimum force applied which induces pain. This measure has proven to be commonly useful in evaluating tenderness symptom. Our aim was to study the intra-examiner reproducibility of PPT measurement, define cutoffs in normal groups, and compare these results with patients with fibromyalgia (FM). Fifty healthy females, 50 healthy males, and 20 patients with FM participated in the study. PPTs were assessed for 18 specific tender point sites by a dolorimeter. The intra-individual coefficient of variation determined by a test-retest PPT measurement procedure with 3-days interval reached, respectively, 17% and 13% in healthy females and males, versus 24% in patients with FM PPTs were significantly lower in healthy females than in healthy males (p < 0.01). Statistical analysis failed to show any differences between the dominant and nondominant side for both normal groups. PPTs were lower over all examined areas in patients with FM than those obtained in healthy females (p < 0.000). Lower cutoff levels were calculated from normal values for all specific tender point sites. On average, 14 tender point sites in patients with FM were under the established lower cutoffs. In conclusion, pressure pain sensitivity was influenced by the anatomical localization of tender point and gender differences. Lowest PPTs were localized in trapezius, occiput, anterior cervical, and second rib. The reduction of total tender point score in patients with FM averaged 60% comparatively with normal values. PPT reproducibility and discrimination between the two groups were optimal for the gluteal and knee sites. (C) 2003 European Federation of Chapters of the International Association for the Study of Pain. Published by Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 91 (12 ULg)
Full Text
Peer Reviewed
See detailTerritorial aggression, circulating levels of testosterone, and brain aromatase activity in free-living pied flycatchers
Silverin, B.; Baillien, M.; Balthazart, Jacques ULg

in Hormones & Behavior (2004), 45(4), 225-234

Testosterone (T) is a critical endocrine factor for the activation of many aspects of reproductive behavior in vertebrates. Castration completely eliminates the display of aggressive and sexual behaviors ... [more ▼]

Testosterone (T) is a critical endocrine factor for the activation of many aspects of reproductive behavior in vertebrates. Castration completely eliminates the display of aggressive and sexual behaviors that are restored to intact level by a treatment with exogenous T. There is usually a tight correlation between the temporal changes in plasma T and the frequency of reproductive behaviors during the annual cycle. In contrast, individual levels of behavioral activity are often not related to plasma T concentration at the peak of the reproductive season suggesting that T is available in quantities larger than necessary to activate behavior and that other factors limit the expression of behavior. There is some indication from work in rodents that individual levels of brain aromatase activity (AA) may be a key factor that limits the expression of aggressive behavior, and in agreement with this idea, many studies indicate that estrogens produced in the brain by the aromatization of T may contribute to the activation of reproductive behavior, including aggression. We investigated here in pied flycatcher (Ficedula hypoleuca) the relationships among territorial aggression, plasma T, and brain AA at the peak of the reproductive season. In a first experiment, blood samples were collected from impaired males holding a primary territory and, I or 2 days later, their aggressive behavior was quantified during standardized simulated territorial intrusions. No relationship was found between individual differences in aggressive behavior and plasma T or dihydrotestosterone levels but a significant negative correlation was observed between number of attacks and plasma corticosterone. In a second experiment, aggressive behavior was measured during a simulated territorial intrusion in 22 impaired males holding primary territories. They were then immediately captured and AA was measured in their anterior and posterior diencephalon and in the entire telencephalon. Five males that had attracted a female (who had started egg-laying) were also studied. The paired males were less aggressive and correlatively had a lower AA in the anterior diencephalon but not in the posterior diencephalon and telencephalon than the 22 birds holding a territory before arrival of a female. In these 22 birds, a significant correlation was observed between number of attacks/min displayed during the simulated territorial intrusion and AA in the anterior diencephalon but no correlation was found between these variables in the two other brain areas. Taken together, these data indicate that the level of aggression displayed by males defending their primary territory may be limited by the activity of the preoptic aromatase, but plasma T is not playing an important role in establishing individual differences in aggression. Alternatively, it is also possible that brain AA is rapidly affected by agonistic interactions and additional work should be carried out to determine whether the correlation observed between brain AA and aggressive behavior is the result of an effect of the enzyme on behavior or vice versa. In any case, the present data show that preoptic AA can change quite rapidly during the reproductive cycle (within a few days after arrival of the female) indicating that this enzymatic activity is able to regulate rapid behavioral transitions during the reproductive cycle in this species. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 37 (0 ULg)
Full Text
Peer Reviewed
See detailFreeze-dried poly(D,L-lactic acid) macroporous guidance scaffolds impregnated with brain-derived neurotrophic factor in the transected adult rat thoracic spinal cord
Patist, Carla M; Borgerhoff Mulder, Masha; Gautier, Sandrine et al

in Biomaterials (2004), 25(9), 1569-1582

The effects of poly(D,L-lactic acid) macroporous guidance scaffolds (foams) with or without brain-derived neurotrophic factor (BDNF) on tissue sparing, neuronal survival, axonal regeneration, and ... [more ▼]

The effects of poly(D,L-lactic acid) macroporous guidance scaffolds (foams) with or without brain-derived neurotrophic factor (BDNF) on tissue sparing, neuronal survival, axonal regeneration, and behavioral improvements of the hindlimbs following implantation in the transected adult rat thoracic spinal cord were studied. The foams were embedded in fibrin glue containing acidic-fibroblast growth factor. One group of animals received fibrin glue with acidic-fibroblast growth factor only. The foams were prepared by a thermally induced polymer-solvent phase separation process and contained longitudinally oriented macropores connected to each other by a network of micropores. Both foams and fibrin only resulted in a similar gliotic and inflammatory response in the cord-implant interfaces. With BDNF foam, up to 20% more NeuN-positive cells in the spinal nervous tissue close to the rostral but not caudal spinal cord-implant interface survived than with control foam or fibrin only at 4 and 8 weeks after implantation. Semithin plastic sections and electron microcopy revealed that cells and axons more rapidly invaded BDNF foam than control foam. Also, BDNF foam contained almost twice as many blood vessels than control foam at 8 weeks after implantation. Tissue sparing was similar in all three implantation paradigms; approximately 42% of tissue was spared in the rostral cord and approximately 37% in the caudal cord at 8 weeks post grafting. The number of myelinated and unmyelinated axons was low and not different between the two types of foams. Many more axons were found in the fibrin only graft. Serotonergic axons were not found in any of the implants and none of the axons regenerated into the caudal spinal cord. The behavioral improvements in the hindlimbs were similar in all groups. These findings indicated that foam is well tolerated within the injured spinal cord and that the addition of BDNF promotes cell survival and angiogenesis. However, the overall axonal regeneration response is low. Future research should explore the use of poly(D,L-lactic acid) foams, with or without axonal growth-promoting factors, seeded with Schwann cells to enhance the axonal regeneration and myelination response. [less ▲]

Detailed reference viewed: 126 (0 ULg)
Full Text
Peer Reviewed
See detailL'oedeme aigu pulmonaire cardiogenique
Stainier, P. Y.; Lancellotti, Patrizio ULg; Smeets, V. et al

in Revue Médicale de Liège (2004), 59(4), 196-200

Acute cardiogenic pulmonary oedema frequently occurs in clinical practice, frequently in an old patient. It consists in acute respiratory distress related to rapid development of transsudative fluid ... [more ▼]

Acute cardiogenic pulmonary oedema frequently occurs in clinical practice, frequently in an old patient. It consists in acute respiratory distress related to rapid development of transsudative fluid within the lung interstitium and alveolar accumulation. It requires rapid assessment and therapy to prevent progression to respiratory failure and cardiovascular collapse. The pathogenesis and identification of the underlying disease process are essential for optimal medical approach. The aetiology is multiple. [less ▲]

Detailed reference viewed: 16 (6 ULg)
Full Text
See detailL’affaire Thone : une « Omerta » bien bavarde
Lanneau, Catherine ULg

in Bulletin de la Société Royale Le Vieux-Liège (2004), XIV(305), 535-551

Detailed reference viewed: 85 (24 ULg)
Full Text
Peer Reviewed
See detailStructure and dynamics of cationic surfactants intercalated in synthetic clays
Müller, Robert; Hrobarikova, Jana; Calberg, Cédric ULg et al

in Langmuir (2004), 20(7), 2982-2985

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailBiochemostratigraphy of the Devonian-Carboniferous boundary Global Stratotype Section and Point, Griotte Formation, La Serre, Montagne Noire, France
Brand, U.; Legrand-Blain, M.; Streel, Maurice ULg

in Palaeogeography Palaeoclimatology Palaeoecology (2004), 205(3-4), 337-357

The Griotte Formation and the base of Bed 89 exposed at La Serre, Montagne Noire, France represent the Global Stratotype Section and Point (GSSP) for the Devonian-Carboniferous (D-C) boundary. This study ... [more ▼]

The Griotte Formation and the base of Bed 89 exposed at La Serre, Montagne Noire, France represent the Global Stratotype Section and Point (GSSP) for the Devonian-Carboniferous (D-C) boundary. This study represents the geochemical work on brachiopods and matrix covering the Siphonodella praesulcata to Siphonodella sulcata Zones at the GSSP. Values of Sr-87/Sr-86 of unaltered brachiopods provide the most powerful biochemostratigraphic tool in recognizing and correlating the Devonian-Carboniferous boundary on a global scale. The frequency and variations of the latest Devonian Sr-87/Sr-86 trend (0.708167 +/- 0.000042) are invariant but the population means are similar (p = 1.000) to the essentially pronounced trend for the post-boundary Carboniferous (0.708165 +/- 0.000054) brachiopods from the GSSP and supplementary sections. The Devonian-Carboniferous boundary Sr-87/Sr-86 value based on unaltered brachiopod samples from within 0.2 m of either side of the boundary is 0.708231 +/- 0.000021 (NBS 987 = 0.710240). Oxygen isotope values of unaltered brachiopods from the Devonian side of the boundary are -1.99 +/- 2.29% (PDB) and those from the Carboniferous side are -3.08 +/- 0.51% (PDB, p = 0.229), which are from within to outside the range recorded in Recent low-latitude counterparts. Carbon isotope values for the Devonian brachiopods with +4.37 +/- 1.69% (PDB) are dissimilar to those from the Carboniferous side of the boundary with +1.93 +/- 0.49% (PDB; p = 0.0001), but both mostly overlap with the range observed in Recent low-latitude brachiopods. The large positive excursions in delta(13)C (Delta5.5%) and 8180 (Delta6.9%) of the unaltered brachiopods reflect changing oceanographic conditions of the La Serre Sea and possibly of the global oceans during the uppermost Middle and Upper S. praesulcata Subzones. Oxygen isotope trend may reflect, in part, a climatic response of a rapid, short and distinct glacial event during the upper Middle S. praesulcata Subzone. This cryogenic event of a larger oceanographic/climatic shift correlates, in part, to the Hangenberg Event sensu lato. Excursions in the seawater-Sr-87 bracket the onset and offset of the glacial event, reflecting changes in continental weathering patterns and processes and with it changes in riverine fluxes. In contrast, the carbon isotope shift although related to the cooling trend is probably associated with a drawdown of atmospheric CO2 and/or burial of organic matter. The biochemostratigraphic values/contents and trends presented in this study, in conjunction with biostratigraphic observations, facilitate the correlation of sequences at Hasselbachtal and Wocklum (Germany), Nanbiancun (China) and Starks Road and Hannibal (Missouri, USA). (C) 2004 Elsevier B.V All rights reserved. [less ▲]

Detailed reference viewed: 41 (1 ULg)
Full Text
Peer Reviewed
See detailEuropean College of Rabbit Medicine and Surgery?
Vindevogel, Henri ULg; Marlier, Didier ULg

in Veterinary Record : Journal of the British Veterinary Association (2004), 154(13), 412-412

Detailed reference viewed: 19 (4 ULg)
Full Text
Peer Reviewed
See detailIsolation of caprine herpesvirus type 1 in Spain
Keuser, V.; Espejo-Serrano, J.; Schynts, F. et al

in Veterinary Record : Journal of the British Veterinary Association (2004), 154(13), 395-399

Two strains of caprine herpesvirus type 1 (CpHV-1) were isolated after the experimental reactivation of two seropositive goats in Spain. Viral DNA from these isolates was compared with DNA from bovine ... [more ▼]

Two strains of caprine herpesvirus type 1 (CpHV-1) were isolated after the experimental reactivation of two seropositive goats in Spain. Viral DNA from these isolates was compared with DNA from bovine herpesvirus type 1 and CpHV-1 reference strains by restriction endonuclease analysis. The two Spanish isolates were closely related but could easily be distinguished from each other and from the reference strains. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailStriatal PSA-NCAM(+) precursor cells from the newborn rat express functional glycine receptors
Nguyen, Laurent ULg; Malgrange, Brigitte ULg; Breuskin, Ingrid ULg et al

in Neuroreport (2004), 15(4), 583-587

Immunocytochemical analysis showed that ionotropic glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum and expressing the polysialylated form of the neural ... [more ▼]

Immunocytochemical analysis showed that ionotropic glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum and expressing the polysialylated form of the neural cell adhesion molecule, both in vitro and in situ. To ascertain whether glycine receptors were functional in vitro, whole-cell patch-clamp recordings demonstrated that glycine triggers inward strychnine-sensitive currents in the majority of these cells. Moreover, we found that glycine receptors expressed by these neurogenic progenitors display intermediate electrophysiological characteristics between those of glycine receptors expressed by neural stem cells and by mature interneurons from the rat striatum. Altogether, the present data show that functional strychnine-sensitive glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum. [less ▲]

Detailed reference viewed: 50 (4 ULg)
Full Text
Peer Reviewed
See detailt-channel unitarity and photon cross sections
Cudell, Jean-René ULg; Martynov, E.; Soyez, G.

in Nuclear Physics B (2004), 682(1-2), 391-420

We analyse the consequences of t-channel unitarity for the photon hadronic cross sections. We show that gamma((*))p and gamma((*))gamma((*)) elastic amplitudes must include the complex-j plane ... [more ▼]

We analyse the consequences of t-channel unitarity for the photon hadronic cross sections. We show that gamma((*))p and gamma((*))gamma((*)) elastic amplitudes must include the complex-j plane singularities associated with hadronic elastic amplitudes, and give the characteristics of possible new singularities. We also show that several different models of hadronic total cross sections can be used to predict the LEP data from the HERA measurements. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 27 (4 ULg)
Full Text
Peer Reviewed
See detailEvaluation of a novel anion-exchange restricted-access sorbent for on-line sample clean-up prior to the determination of acidic compounds in plasma by liquid chromatography
Rbeida, O.; Christiaens, B.; Hubert, Philippe ULg et al

in Journal of Chromatography. A (2004), 1030(1-2), 95-102

A new kind of silica-based restricted-access material (RAM) with anionic properties has been tested in pre-columns for on-line solid-phase extraction of acidic compounds from directly injected plasma ... [more ▼]

A new kind of silica-based restricted-access material (RAM) with anionic properties has been tested in pre-columns for on-line solid-phase extraction of acidic compounds from directly injected plasma samples prior to their determination by reversed-phase liquid chromatography (LC), using the column-switching technique. The outer surface of the porous RAM particles contains hydrophilic diol groups while diethylaminoethyl (DEAE) groups are bound to the internal surface which gives the sorbent the properties of a weak anion exchanger towards low-molecular-mass compounds. Due to an appropriate pore diameter (about 6 nm), macromolecules, such as proteins, are physically excluded from the pores and flushed directly out during the sample clean-up process, while small compounds have access to the inner surface and can be retained mainly by electrostatic interactions. The retention capability of this novel packing material has been tested for some hydrophilic acidic compounds such as aspartic acid, glutamic acid, ascorbic acid and acetylcysteine as well as for some more hydrophobic drugs such as naproxen, ibuprofen and diclofenac, used as model compounds. The influence of the composition of the washing liquid on the retention of the analytes in the pre-column has been investigated. The efficiency of the sorbent to clean-up complex matrices was also tested using human plasma and urine samples. A generic washing liquid composition was then selected in order to obtain efficient and selective sample clean-up as well as a high recovery of the acidic analytes. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 48 (7 ULg)
Full Text
Peer Reviewed
See detailSilica reinforced triblock copolymer gels
Theunissen, E.; Overbergh, N.; Reynaers, H. et al

in Polymer (2004), 45(6), 1857-1865

The effect of silica and polymer coated silica particles as reinforcing agents on the structural and mechanical properties of polystyrene-poly(ethylene/butylene)-polystyrene (PS-PEB-PS) triblock gel has ... [more ▼]

The effect of silica and polymer coated silica particles as reinforcing agents on the structural and mechanical properties of polystyrene-poly(ethylene/butylene)-polystyrene (PS-PEB-PS) triblock gel has been investigated. Different types of chemically modified silica have been compared in order to evaluate the influence of the compatibility between gel and filler. Time-resolved SANS and small-angle X-ray scattering (SAXS) shows that the presence of silica particles affects the ordering of the polystyrene domains during gelsetting. The scattering pattern of silica-reinforced gels reveals strong scattering at very low q, but no structure and formfactor information. However, on heating above the viscoelastic to plastic transition, the 'typical' scattering pattern of the copolymer gel builds-up. All reinforced gels are strengthened by the addition of the reinforcing agent. The transitions from a viscoclastic rubber to a plastic fluid and from a plastic fluid to a viscoelastic liquid are shifted to more elevated temperatures when silica is added to the triblock copolymer gel. [less ▲]

Detailed reference viewed: 21 (0 ULg)
Full Text
Peer Reviewed
See detailImpaired performance in a working memory binding task in patients with schizophrenia
Burglen, F.; Marczewski, P.; Mitchell, K. J. et al

in Psychiatry Research (2004), 125(3), 247-255

This study investigated feature binding in a working memory task in patients with schizophrenia and in normal controls. Twenty-five patients and 25 controls participated. On each trial, three drawings of ... [more ▼]

This study investigated feature binding in a working memory task in patients with schizophrenia and in normal controls. Twenty-five patients and 25 controls participated. On each trial, three drawings of familiar objects were presented sequentially, each in a different cell of a 3 x 3 grid. In different blocks of trials, participants remembered either individual features (object and location conditions) or an object and its location (combination condition). The results showed that patients were slower and less accurate than controls under all conditions. Accuracy of both groups, was reduced in the combination condition relative to the single-feature conditions, but patients showed disproportionately poorer performance in the combination condition than in the object and location conditions. Thus, patients with schizophrenia exhibit deficits in working memory, particularly when the task requires binding objects to their locations. This finding demonstrates that processes that establish coherent and temporary episodic representations in working memory are impaired in schizophrenia. (C) 2003 Elsevier Ireland Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailEffects of atrazine on sex steroid dynamics, plasma vitellogenin concentration and gonad development in adult goldfish (Carassius auratus)
Spano, L.; Tyler, C. R.; van Aerle, R. et al

in Aquatic Toxicology (2004), 66(4), 369-379

Sexually mature goldfish (Carassius auratus) of both sexes were exposed to two doses (100 and 1000 mug/l) of the widely used herbicide atrazine (2-chloro-4-ethylamino-6-isolropylamino-s-triazine) for a ... [more ▼]

Sexually mature goldfish (Carassius auratus) of both sexes were exposed to two doses (100 and 1000 mug/l) of the widely used herbicide atrazine (2-chloro-4-ethylamino-6-isolropylamino-s-triazine) for a period of 21 days and effects on the concentrations of gonad and plasma sex steroids (testosterone (T), 17beta-estradiol (E2) and 11-ketotestosterone (11-KT)), plasma vitellogenin (VTG) and gonad histo-morphology assessed. Atrazine did not show any obvious estrogenic effect in males, as determined by a lack of vitellogenin induction. There were, however, effects of atrazine on plasma androgen concentrations (androgen dynamics) and tissue (plasma and gonad) estrogen concentrations in male goldfish; exposure to 1000 mug/l atrazine induced suppression in both testosterone and 11-ketotestosterone, and resulted in elevated 17beta-estradiol, after 21 day of exposure. Further, these suppressive effects on plasma androgens and the induction in estrogen were dose- and time-related. The highest atrazine exposure dose induced structural disruption in the testis and both 100 and 1000 mug/l induced elevated levels of atresia in ovaries. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detailPLA-coated gold nanoparticles for the labeling of PLA biocarriers
Qiu, Hongjin; Rieger, Jutta ULg; Gilbert, Bernard ULg et al

in Chemistry of Materials (2004), 16(5), 850-856

POly-DL-lactide end-capped by a protected thiol was synthesized by bulk ring-opening polymerization (ROP) of DL-lactide initiated by the reaction product of aluminum isopropoxide [Al (iOPr) (3)] with ... [more ▼]

POly-DL-lactide end-capped by a protected thiol was synthesized by bulk ring-opening polymerization (ROP) of DL-lactide initiated by the reaction product of aluminum isopropoxide [Al (iOPr) (3)] with alpha- (2,4-dinitrophenylsulfenyl) ethanol. After the thiol deprotection, PLA-SH was used to stabilize gold nanoparticles. Either these nanoparticles were prepared in the presence of PLA-SH, or PLA-SH was substituted for part of the undecanethiol (C11SH) that stabilized preformed gold nanoparticles. In contrast to C11SH-coated nanoparticles, those stabilized by PLA-SH were successfully entrapped into 100-nm PLA nanocarriers prepared by nanoprecipitation. This is an easy technique to label PLA biocarriers and therefore trace their fate in vivo. [less ▲]

Detailed reference viewed: 61 (2 ULg)
Full Text
See detailnote sous arrêt du 4 mars 2003 "FENIN c/ Commission"
Petit, Nicolas ULg

in Revue du Droit de l'Union Européenne (2004)

Résumé et analyse de l'affaire FENIN contre Commission (affaire T-399/99)

Detailed reference viewed: 116 (3 ULg)
Full Text
Peer Reviewed
See detailSolid-state NMR study of poly(epsilon-caprolactone)/clay nanocomposites
Calberg, Cédric ULg; Jérôme, Robert ULg; Grandjean, Jean ULg

in Langmuir (2004), 20(5), 2039-2041

Detailed reference viewed: 35 (0 ULg)
Full Text
Peer Reviewed
See detailInfluence of substrate hydrophobicity on the adsorption of an amphiphilic diblock copolymer
De Cupere, V. M.; Gohy, Jean-François; Jérôme, Robert ULg et al

in Journal of Colloid and Interface Science (2004), 271(1), 60-68

The adsorption of poly(tert-butylmethacrylate)-block-poly(2-(dimethylamino-ethyl) methacrylate) (PtBUMA-b-PDMAEMA) was studied by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM ... [more ▼]

The adsorption of poly(tert-butylmethacrylate)-block-poly(2-(dimethylamino-ethyl) methacrylate) (PtBUMA-b-PDMAEMA) was studied by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) analysis performed on dried samples. The copolymer was dissolved in toluene at concentrations below (0.01 wt%) and above (0.05 and 1 wt%) the CMC; silicon (SiOH) and CH3-grafted silicon (SiCH3) were used as substrates. Whatever the concentration and the substrate, a layer of individual copolymer molecules, 1.5-3 nm thick, formed rapidly. The adsorbed amount was slightly higher and the resistance to AFM tip scraping was stronger on SiOH than oil SiCH3. This is attributed to hydrogen bonding between the PDMAEMA block and the OH groups of the silicon surface, leading to polarization of the adsorbed layer. Above the CMC, on SiOH, randomly scattered dot-like features (about 5 nm high) observed by AFM were attributed to individual micelles, which were not displaced by drying. On SiCH3, the particles found on the top of the adsorbed layer were micelle aggregates, about 50 nm thick, the lateral size of which was strongly influenced by the rate of drying. This further difference between SiCH3 and SiOH is tentatively attributed to the exposure of PDMAEMA by the adsorbed layer formed on SiCH3, while only PtBUMA would be exposed by the layer adsorbed on SiOH. The red blood cell shape and the size of the micelles observed in single layers indicate that the PtBUMA corona was not made compact as a result of drying, (C) 2003 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 29 (3 ULg)
Full Text
Peer Reviewed
See detailAmphiphilic block copolymers of high-molecular-weight poly(ethylene oxide) and either epsilon-caprolactone or gamma-methyl-epsilon-caprolactone: Synthesis and characterization
Vangeyte, Patrick; Jérôme, Robert ULg

in Journal of Polymer Science. Part A, Polymer Chemistry (2004), 42(5), 1132-1142

Ethylene oxide (EO) has been block-polymerized with both epsilon-caprolactone (epsilon-CL) and gamma-methyl-epsilon-caprolactone (MCL) through the combination of the anionic polymerization of EO and the ... [more ▼]

Ethylene oxide (EO) has been block-polymerized with both epsilon-caprolactone (epsilon-CL) and gamma-methyl-epsilon-caprolactone (MCL) through the combination of the anionic polymerization of EO and the ring-opening polymerization (ROP) of epsilon-CL and MCL. omega-Hydroxyl poly(ethylene oxide) has been reacted with triethylaluminum (OH/Al = 1) and converted into a macroinitiator for ROP of epsilon-CL and MCL. In toluene at room temperature, this polymerization leads to a bimodal molecular weight distribution as a result of monomer insertion in only some of the aluminum alkoxide bonds. However, in a more polar solvent (methylene chloride) added with 1 equiv of a Lewis base (pyridine), the expected diblock is formed selectively, and this indicates that aggregation of the active species in toluene is responsible for a macroinitiator efficiency of less than 1. A series of amphiphilic diblock copolymers with poly(epsilon-caprolactone) (semicrystalline) and poly(gamma-methyl-epsilon-caprolactone) (amorphous) as the hydrophobic blocks have been prepared and characterized with size exclusion chromatography, H-1 NMR, IR, and wide-angle X-ray scattering. [less ▲]

Detailed reference viewed: 58 (1 ULg)
Full Text
Peer Reviewed
See detailLong-term multicolour photometry and high-resolution spectroscopy of the two gamma Doradus stars HD 12901 and HD 48501
Aerts, C.; Cuypers, J.; De Cat, P. et al

in Astronomy and Astrophysics (2004), 415

We gathered long-term multicolour Geneva UB[SUB]1[/SUB]BB[SUB]2[/SUB]V[SUB]1[/SUB]VG photometric and high-resolution (R=40 000) spectroscopic data of the two gamma Doradus stars HD 12901 and HD 48501. The ... [more ▼]

We gathered long-term multicolour Geneva UB[SUB]1[/SUB]BB[SUB]2[/SUB]V[SUB]1[/SUB]VG photometric and high-resolution (R=40 000) spectroscopic data of the two gamma Doradus stars HD 12901 and HD 48501. The photometry reveals three frequencies for each of the two stars: f[SUB]1[/SUB]=1.21563 c d[SUP]-1[/SUP], f[SUB]2[/SUB]=1.39594 c d[SUP]-1[/SUP] and f[SUB]3[/SUB]=2.18636 c d[SUP]-1[/SUP] for HD 12901 and f[SUB]1[/SUB]=1.09408 c d[SUP]-1[/SUP], f[SUB]2[/SUB]=1.29054 c d[SUP]-1[/SUP] and f[SUB]3[/SUB]=1.19924 c d[SUP]-1[/SUP] for HD 48501. The photometric amplitude is each time largest in the Geneva B[SUB]1[/SUB] filter and the variations in all the different filters are perfectly in phase within the measurement errors. Mode identification points out that the six modes are all l=1 modes and that the non-adiabatic temperature variations are extremely small, in contradiction to current theoretical predictions. Our spectra show that all the observed frequencies are intrinsic to the stars and cannot be due to binarity. We detect clear line-profile variations at low amplitude (<1 km s[SUP]-1[/SUP]) due to the oscillations of both targets. The estimated v sin i from the spectra are Ë 53 km s[SUP]-1[/SUP] for HD 12901 and Ë 29 km s[SUP]-1[/SUP] for HD 48501. It is at present unclear if the triplet-like structure for HD 48501 is the consequence of rotational splitting or of the large separation expected for high-order gravity modes in the asymptotic regime. Based on observations gathered with the Swiss 0.7 m telescope equipped with the photometer P 7 and with the Swiss 1.2 m Euler telescope equipped with the spectrograph CORALIE, both situated at La Silla, Chile. Reduced data available upon request from the first author. [less ▲]

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailAssociation between polymorphism in IgG Fc receptor IIIa coding gene and biological response to infliximab in Crohn's disease
Louis, Edouard ULg; El Ghoul, Z.; Vermeire, S. et al

in Alimentary Pharmacology & Therapeutics (2004), 19(5), 511-519

AIM: To test the hypothesis of an association between polymorphism in FCGR3A (the gene coding for FcgammaRIIIa, which is expressed on macrophages and natural killer cells, is involved in antibody ... [more ▼]

AIM: To test the hypothesis of an association between polymorphism in FCGR3A (the gene coding for FcgammaRIIIa, which is expressed on macrophages and natural killer cells, is involved in antibody-dependent cell-mediated cytotoxicity and has recently been associated with a positive response to rituximab, a recombinant immunoglobulin G1 antibody used in non-Hodgkin's lymphomas) and response to infliximab in Crohn's disease. METHODS: FCGR3A-158 polymorphism was determined using an allele-specific polymerase chain reaction assay in 200 Crohn's disease patients who had received infliximab for either refractory luminal (n = 142) or fistulizing (n = 58) Crohn's disease. Clinical and biological responses (according to C-reactive protein levels) were assessed in 200 and 145 patients, respectively. RESULTS: There were 82.9% clinical responders in V/V patients vs. 72.7% in V/F and F/F patients (N.S.). Globally, the decrease in C-reactive protein was significantly higher in V/V patients than in F carriers (P = 0.0078). A biological response was observed in 100% of V/V patients, compared with 69.8% of F carriers (P = 0.0002; relative risk, 1.43; 95% confidence interval, 1.27-1.61). In the sub-group of patients with elevated C-reactive protein before treatment, the multivariate analysis selected the use of immunosuppressive drugs and FCGR3A genotype as independent factors influencing the clinical response to infliximab (P = 0.003). CONCLUSION: Crohn's disease patients with FCGR3A-158 V/V genotype have a better biological and, possibly, clinical response to infliximab. [less ▲]

Detailed reference viewed: 32 (2 ULg)
Full Text
Peer Reviewed
See detailIncreased IL-6 and TGF-beta(1) concentrations in bronchoalveolar lavage fluid associated with thoracic radiotherapy
Barthelemy-Brichant, Nicole ULg; Bosquee, Lionel; Cataldo, Didier ULg et al

in International Journal of Radiation, Oncology, Biology, Physics (2004), 58(3), 758-767

PURPOSE: To assess, in lung cancer patients, the effects of thoracic radiotherapy (RT) on the concentrations of transforming growth factor-beta(1) (TGF-beta(1)) and interleukin-6 (IL-6) in the ... [more ▼]

PURPOSE: To assess, in lung cancer patients, the effects of thoracic radiotherapy (RT) on the concentrations of transforming growth factor-beta(1) (TGF-beta(1)) and interleukin-6 (IL-6) in the bronchoalveolar lavage (BAL) fluid. METHODS AND MATERIALS: Eleven patients with lung cancer requiring RT as part of their treatment were studied. BAL was performed bilaterally before, during, and 1, 3, and 6 months after RT. Before each BAL session, the patient's status was assessed clinically using pulmonary function tests and an adapted late effects on normal tissue-subjective, objective, management, analytic (LENT-SOMA) scale, including subjective and objective alterations. The National Cancer Institute Common Toxicity Criteria were used to grade pneumonitis. The TGF-beta(1) and IL-6 levels in the BAL fluid were determined using the Easia kit. RESULTS: The TGF-beta(1) and IL-6 concentrations in the BAL fluid recovered from the irradiated areas were significantly increased by thoracic RT. The increase in TGF-beta(1) levels tended to be greater in the group of patients who developed severe pneumonitis. In the BAL fluid from the nonirradiated areas, the TGF-beta(1) and IL-6 concentrations remained unchanged. CONCLUSION: The observed increase in TGF-beta(1) and IL-6 concentrations in the BAL fluid recovered from the irradiated lung areas demonstrated that these cytokines may contribute to the process leading to a radiation response in human lung tissue. [less ▲]

Detailed reference viewed: 78 (9 ULg)
Full Text
Peer Reviewed
See detailSolar wind control of auroral substorm onset locations observed with the IMAGE-FUV imagers
Gérard, Jean-Claude ULg; Hubert, Benoît ULg; Grard, Aline ULg et al

in Journal of Geophysical Research. Space Physics (2004), 109

The FUV imagers on board the IMAGE satellite provide multispectral snapshots of the polar region every 2 min. The combination of the Wide-Angle Imaging Camera (WIC) with SI12 (Doppler shifted Lyman-alpha ... [more ▼]

The FUV imagers on board the IMAGE satellite provide multispectral snapshots of the polar region every 2 min. The combination of the Wide-Angle Imaging Camera (WIC) with SI12 (Doppler shifted Lyman-alpha) and SI13 (135.6 nm) spectral imagers is used to discriminate between the electron and the proton aurora. We describe a statistical study of the location of 78 substorms observed close to the 2000-2001 winter solstice. The latitudinal distribution of the onsets observed with WIC is asymmetric with a median at 65.6° MLAT and a full width at half maximum (FWHM) of 3.5°. Their local time distribution is concentrated between 2000 and 0200 MLT with a median at 23.4 +/- 0.3 hours MLT and a FWHM of 1.8 hours. No statistically significant difference is found in the spatial distribution of the proton and electron onsets. All onsets take place within a region of preexisting proton precipitation, indicating that substorm initiation occurs in regions of stretched but dipole-like field lines that cross the equatorial plane close to the Earth. Latitudes of substorm onsets are located at a variable distance from the poleward FUV auroral boundary but remain at a nearly constant distance from the equatorward limit of both proton and electron auroral ovals. The magnetic latitudes of the onsets are correlated with some of the solar wind plasma properties measured by the ACE satellite prior to the substorm breakup. In particular, a clear anticorrelation is found between the onset MLAT and the 1-hour averaged solar wind dynamic pressure. A decrease of the onset latitude is also observed for larger B intensity values. No dependence of the onset MLT on the solar wind speed is observed, in contrast to the relationship expected from the thermal catastrophe model for substorm initiation. Our results are in agreement with models locating the initial instability in the near magnetosphere such as the near-Earth current disruption models. [less ▲]

Detailed reference viewed: 47 (6 ULg)
Full Text
See detailRésultats d'un inventaire des reptiles par la méthode des "Plaques Refuges" en Région Wallonne
Graitson, Eric ULg

in Natura Mosana (2004), 56(4), 73-83

Detailed reference viewed: 179 (12 ULg)
Full Text
Peer Reviewed
See detailFinite-element analysis of a double-winding induction motor with a special rotor bars topology
Oliveira, Ana Margarida; Kuo-Peng, Patrick; Sadowski, Nelson et al

in IEEE Transactions on Magnetics (2004), 40(2, Part 2), 770-773

A model allowing the simulation of induction machines with different rotor bars configurations is presented. The electrical machine is modeled by using two-dimensional (2-D) finite-element method and its ... [more ▼]

A model allowing the simulation of induction machines with different rotor bars configurations is presented. The electrical machine is modeled by using two-dimensional (2-D) finite-element method and its equations are directly coupled with those of the circuit. A general and new contribution is added for the consideration of special rotor bars topologies. The movement is taken into account by means of the moving band technique, the Maxwell stress tensor, and the mechanical oscillation equation. The model is applied to a brushless doubly fed induction motor. [less ▲]

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailImaging a cognitive model of apraxia: The neural substrate of gesture-specific cognitive processes
Peigneux, Philippe ULg; Van der Linden, Martial ULg; Garraux, Gaëtan ULg et al

in Human Brain Mapping (2004), 21(3), 119-142

The present study aimed to ascertain the neuroanatomical basis of an influential neuropsychological model for upper limb apraxia [Rothi LJ, et al. The Neuropsychology of Action. 1997. Hove, UK: Psychology ... [more ▼]

The present study aimed to ascertain the neuroanatomical basis of an influential neuropsychological model for upper limb apraxia [Rothi LJ, et al. The Neuropsychology of Action. 1997. Hove, UK: Psychology Press]. Regional cerebral blood flow was measured in healthy volunteers using (H2O)-O-15 PET during performance of four tasks commonly used for testing upper limb apraxia, i.e., pantomime of familiar gestures on verbal command, imitation of familiar gestures, imitation of novel gestures, and an action-semantic task that consisted in matching objects for functional use. We also re-analysed data from a previous PET study in which we investigated the neural basis. of the visual analysis of gestures. First; we found that two sets of discrete brain areas are predominantly engaged in the imitation of familiar and novel gestures, respectively. Segregated brain activation for novel gesture mutation concur with neuropsychological reports to support the hypothesis that knowledge about the organization of the human body mediates the transition from visual perception to motor execution when imitating novel gestures [Goldenberg Neuropsychologia 1995;35.63-72]. Second, conjunction analyses revealed distinctive neural bases for most of the gesture-specific cognitive processes proposed in this cognitive model of upper limb apraxia. However, a functional analysis of brain imaging data suggested that one single memory store may be used for "to be-perceived" and "to-be-produced" gestural representations, departing from Rothi et al.'s proposal. Based on the above considerations, we suggest and discuss a revised model for upper limb apraxia that might best account for both brain imaging findings and neuropsychological dissociations reported in the apraxia literature. (C) 2004 Wiley-Liss, Inc. [less ▲]

Detailed reference viewed: 112 (4 ULg)
Full Text
Peer Reviewed
See detailEvolutionary mode, tempo, and phylogenetic association of continuous morphological traits in the aquatic moss genus Amblystegium
Vanderpoorten, Alain ULg; Jacquemart, A. L.

in Journal of Evolutionary Biology (2004), 17(2), 279-287

Evolutionary significance of morphological characters that have traditionally been used for species delineation in the aquatic moss genus Amblystegium was tested by partitioning the environmentally and ... [more ▼]

Evolutionary significance of morphological characters that have traditionally been used for species delineation in the aquatic moss genus Amblystegium was tested by partitioning the environmentally and genetically induced morphological variation and focusing on morphological evolution using comparative methods. Cultivation experiments under controlled condition showed that most of the morphological variation in nature resulted from plasticity. Information regarding genetically fixed morphological variation and genetic similarity derived from polymorphic inter-simple sequence repeat markers was combined into an explicit model of morphological evolution. Maximum likelihood estimates of the model parameters indicated that evolution of most characters tended to accelerate in the most recent taxa and was often independent from the phylogeny. Constraining the different characters to be independent from each other most often produced a less likely result than when the characters were free to evolve in a correlated fashion. Thus, the morphological characters that have traditionally been used to circumscribe different Amblystegium species lack the independence, diagnostic value for specific lineages, and stability that would be required for distinguishing different species. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailDeletion of a cyclic AMP receptor protein homologue diminishes germination and affects morphological development of Streptomyces coelicolor
Derouaux, Adeline ULg; Halici, S.; Nothaft, H. et al

in Journal of Bacteriology (2004), 186(6), 1893-1897

Open reading frame SCO3571 of Streptomyces coelicolor encodes a protein of the cyclic AMP (cAMP) receptor protein (CRP) superfamily of regulatory proteins. A mutant revealed a dramatic defect in ... [more ▼]

Open reading frame SCO3571 of Streptomyces coelicolor encodes a protein of the cyclic AMP (cAMP) receptor protein (CRP) superfamily of regulatory proteins. A mutant revealed a dramatic defect in germination, followed by growth delay and earlier sporulation. This phenotype correlates with those of an adenylate cyclase (cya) mutant that cannot synthesize cAMP. This finding suggests that S. coelicolor may use a Cya-cAMP-CRP system to trigger complex physiological processes such as morphogenesis. [less ▲]

Detailed reference viewed: 24 (3 ULg)
Full Text
Peer Reviewed
See detailNeurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines
Hansenne, Isabelle ULg; Rasier, G.; Charlet-Renard, C. et al

in Clinical & Developmental Immunology (2004), 11(1), 45-51

Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors ... [more ▼]

Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors belong to the G protein-linked seven-transmembrane receptor superfamily and are encoded by four distinct genes, OTR, V1R, V2R and V3R. The objective of this study was to identify the nature of neurohypophysial receptor in thymic T cell subsets purified by immunomagnetic selection, as well as in murine thymic lymphoma cell lines RL12-NP and BW5147. OTR is transcribed in all thymic T cell subsets and T cell lines, while V3R transcription is restricted to CD4+CD8+ and CD8+ thymic cells. Neither V1R nor V2R transcripts are detected in any kind of T cells. The OTR protein was identified by immunocytochemistry on thymocytes freshly isolated from C57BL/6 mice. In murine fetal thymic organ cultures, a specific OTR antagonist does not modify the percentage of T cell subsets, but increases late T cell apoptosis further evidencing the involvement of OT/OTR signaling in the control of T cell proliferation and survival. According to these data, OTR and V3R are differentially expressed during T cell ontogeny. Moreover, the restriction of OTR transcription to T cell lines derived from thymic lymphomas may be important in the context of T cell leukemia pathogenesis and treatment. [less ▲]

Detailed reference viewed: 28 (2 ULg)
Full Text
Peer Reviewed
See detailRelationship between changes in biochemical markers of bone turnover and BMD to predict vertebral fracture risk
Sarkar, S.; Reginster, Jean-Yves ULg; Crans, G. G. et al

in Journal of Bone and Mineral Research (2004), 19(3), 394-401

The change in BMD is a poor predictor of vertebral fracture risk after raloxifene treatment. One-year percent change in bone turnover and BMD was used to predict vertebral fracture risk. The percent ... [more ▼]

The change in BMD is a poor predictor of vertebral fracture risk after raloxifene treatment. One-year percent change in bone turnover and BMD was used to predict vertebral fracture risk. The percent change in osteocalcin was determined to be a better predictor of vertebral fracture risk than BMD. Introduction: The association between baseline BMD and fracture risk is well understood. However, the relationship between changes in BMD and fracture risk is not well defined. It has previously been demonstrated that BMD change was a poor predictor of vertebral fracture risk in raloxifene-treated women, whereas bone turnover markers were significantly associated with fracture risk. In the current analysis, we explore the prediction of vertebral fracture risk using changes in both BMD and bone turnover. Materials and Methods: The Multiple Outcomes of Raloxifene Evaluation (MORE) trial was a randomized, placebo-controlled trial of 7705 women with osteoporosis treated with raloxifene 60 or 120 mg/day for 3 years. Markers of bone turnover were measured in one-third of the study population (n = 2503), and the present analyses include these women. Logistic regression models were constructed using one-year percent changes in BMD and bone turnover and relevant baseline demographics to predict the risk of vertebral fracture with pooled raloxifene therapy at 3 years. All covariates were standardized before modeling to facilitate direct comparisons between changes in BMD and bone turnover. Results and Conclusion: Prevalent vertebral fracture status (p < 0.0001), baseline lumbar spine BMD (p < 0.0001), and number of years postmenopausal (p = 0.0005) were independent predictors of fracture risk in raloxifene-treated patients. Therapy-by-change in femoral neck BMD (p = 0.02) and therapy-by-change in osteocalcin (OC; p = 0.01) were also significant for all treatment groups, indicating that changes in BMD and OC have different effects on fracture risk for the placebo and pooled raloxifene groups. The final model included significant baseline variables and change in OC (p = 0.01), whereas change in femoral neck BMD was not significant. After adjustment of each significant baseline variable, the percent change in OC was better able to predict the reduction in vertebral fracture risk than the percent change in femoral neck BMD in patients treated with raloxifene. [less ▲]

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailInteractions between penicillin-binding proteins (PBPs) and two novel classes of PBP inhibitors, arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-ones
Zervosen, Astrid ULg; Lu, Wei-Ping; Chen, Zhouliang et al

in Antimicrobial Agents and Chemotherapy (2004), 48(3), 961-969

Several non-beta-lactam compounds were active against various gram-positive and gram-negative bacterial strains. The MICs of arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-ones were lower ... [more ▼]

Several non-beta-lactam compounds were active against various gram-positive and gram-negative bacterial strains. The MICs of arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-ones were lower than those of ampicillin and cefotaxime for methicillin-resistant Staphylococcus aureus M1339 and vancomycin-resistant Enterococcus faecium EF12. Several compounds were found to inhibit the cell wall synthesis of S. aureus and the last two steps of peptidoglycan biosynthesis catalyzed by ether-treated cells of Escherichia coli or cell wall membrane preparations of Bacillus megaterium. The effects of the arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-one derivatives on E. coli PBP 3 and PBP 5, Streptococcus pneumoniae PBP 2xS (PBP 2x from a penicillin-sensitive strain) and PBP 2xR (PBP 2x from a penicillin-resistant strain), low-affinity PBP 2a of S. aureus, and the Actinomadura sp. strain R39 and Streptomyces sp. strain R61 DD-peptidases were studied. Some of the compounds exhibited inhibitory activities in the 10 to 100 muM concentration range. The inhibition of PBP 2xS by several of them appeared to be noncompetitive. The dissociation constant for the best inhibitor (K-i = 10 muM) was not influenced by the presence of the substrate. [less ▲]

Detailed reference viewed: 37 (0 ULg)
Full Text
Peer Reviewed
See detailHormonal and temperature responses to flesinoxan in normal volunteers: an antagonist study
Pitchot, William ULg; Wauthy, Jacques ULg; Legros, Jean-Jacques ULg et al

in European Neuropsychopharmacology (2004), 14(2), 151-155

RATIONALE: Flesinoxan is a highly potent and selective 5-HT1A agonist. In a recent study, in normal volunteers, flesinoxan induced a significant and dose-dependent increase in adrenocorticotropic hormone ... [more ▼]

RATIONALE: Flesinoxan is a highly potent and selective 5-HT1A agonist. In a recent study, in normal volunteers, flesinoxan induced a significant and dose-dependent increase in adrenocorticotropic hormone (ACTH), cortisol, prolactin (PRL), growth hormone (GH) and a decrease in body temperature. OBJECTIVES: In order to better define the role of 5-HT receptor subtypes in response to flesinoxan, we assessed the influence of 5-HT1A and 5-HT2 antagonists on hormonal and temperature responses to flesinoxan. METHODS: Hormonal and temperature responses were studied in 6 volunteers with or without pretreatment with pindolol (30 mg p.o.), a 5-HT1A antagonist, or ritanserin (10 mg p.o.), a selective 5-HT2 antagonist, using a double-blind crossover design. RESULTS: Pindolol significantly antagonized ACTH, PRL, GH and temperature responses to flesinoxan and ritanserin exhibited similar activity on PRL and ACTH responses. CONCLUSIONS: These results show the role of 5-HT1A mechanisms in the PRL, ACTH, GH, and temperature responses to flesinoxan, and the role of 5-HT2 mechanisms in PRL and ACTH responses. Therefore, they confirm the interest of flesinoxan as a 5-HT neuroendocrine probe. [less ▲]

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailModeling of 3-D stranded inductors with the magnetic vector potential formulation and spatially dependent turn voltages of reduced support
Dular, Patrick ULg; Gyselinck, Johan

in IEEE Transactions on Magnetics (2004), 40(2, Part 2), 1298-1301

An alternative method is developed to take three-dimensional stranded inductors into account with the magnetic vector potential magnetodynamic formulation. The definition of the turn voltages as a ... [more ▼]

An alternative method is developed to take three-dimensional stranded inductors into account with the magnetic vector potential magnetodynamic formulation. The definition of the turn voltages as a continuous spatially dependent quantity of reduced support enables us to simplify the turn circuit relations, relating currents and turn voltages. The continuous extension of these relations for all the wire turns then reduces the bandwidth in the system matrix in comparison with the usual method. The voltage of each turn is, moreover, directly known and, hence, does not require a postprocessing. [less ▲]

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailFronts in the Southern Indian Ocean as inferred from satellite sea surface temperature data
Kostianoy, Andrey G.; Ginzburg, Anna I.; Frankignoulle, M. et al

in Journal of Marine Systems (2004), 45(1-2), 55-73

Sea surface temperature (SST) derived from the weekly measurements made by the Advanced Very High Resolution Radiometers (AVHRR) of NOAA satellites was used to investigate the structure and space-time ... [more ▼]

Sea surface temperature (SST) derived from the weekly measurements made by the Advanced Very High Resolution Radiometers (AVHRR) of NOAA satellites was used to investigate the structure and space-time variability of large-scale fronts in the Southern Indian Ocean (30-60degreesS and 20-150degreesE) during the period of 1997-1999. Monthly SST gradient maps provided an overall view of five basic fronts: the North and South Subtropical fronts (NSTF and SSTF, respectively), the Agulhas Front (AF), the Subantarctic Front (SAF), and the Polar Front (PF). Mean location of the fronts and associated SST and SST gradients with corresponding standard deviations were calculated at each 10degrees-spaced longitude. A double structure of the NSTF, SAF, and PF was demonstrated as well as the meandering of all fronts with amplitudes of 2-5degrees in latitude and wavelength of several degrees in longitude. Convergence and transient interaction between neighboring fronts appear to occur not only in the Crozet and Kerguelen regions, but in other regions as well. The mean locations and SST range of every front are in good agreement with previous work based on hydrographic surveys [J. Geophys. Res. 101 (1996) 3675], although some details are different (in particular, the larger zonal extent of the NSTF and the wider frontal SST ranges than previously observed). A good correspondence of the measurements made during two hydrographic surveys in the Kerguelen region (22 January-3 February 1999) and in the Tasmania region (3-22 March 1998) with satellite SST and SST gradient maps was found. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 71 (7 ULg)
Full Text
See detailUn bilan des vaccinations en 2002-2003
Miermans, Marie-Christine ULg

in Promouvoir la santé à l'école (2004), (5), 2-3

Detailed reference viewed: 29 (11 ULg)
Full Text
Peer Reviewed
See detailImproved antigenic methods for differential diagnosis of bovine, caprine, and cervine alphaherpesviruses related to bovine herpesvirus 1
Keuser, V.; Schynts, F.; Detry, Bruno et al

in Journal of Clinical Microbiology (2004), 42(3), 1228-1235

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four ... [more ▼]

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four other alphaherpesviruses of ruminants-namely, BoHV-5, caprine herpesvirus 1 (CpHV-1), cervine herpesvirus 1 (CvHV-1) and CvHV-2-diagnostic tests able to discriminate BoHV-1 from these related viruses are needed to avoid misdiagnosis, especially because some of these viruses are able to cross the species barrier. In this study, murine monoclonal antibodies (MAbs) specific for BoHV-1, BoHV-5, CpHV-1, CvHV-1, and CvHV-2 were produced with the aim of setting up an immunofluorescence assay able to discriminate between these related herpesviruses. Produced MAbs were selected for their viral specificity by enzyme-linked immunosorbent assay and indirect immunofluorescence staining of virus-infected cells. Radioimmunoprecipitation characterization of the selected MAbs revealed that four of them are directed against glycoprotein C (gC) and one of them is directed against gD of these related viruses. The obtained results demonstrate that the antibodies produced allow an unambiguous discrimination of each of the four alphaherpesviruses related to BoHV-1. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailDifferential effects of testosterone on neuronal populations and their connections in a sensorimotor brain nucleus controlling song production in songbirds: a manganese enhanced-magnetic resonance imaging study
Van Meir, V.; Verhoye, M.; Absil, Philippe ULg et al

in Neuroimage (2004), 21(3), 914-923

Nucleus HVC (formerly called high vocal center) of songbirds contains two types of projecting neurons connecting HVC respectively to the nucleus robustus archistriatalis, RA, or to area X. These two ... [more ▼]

Nucleus HVC (formerly called high vocal center) of songbirds contains two types of projecting neurons connecting HVC respectively to the nucleus robustus archistriatalis, RA, or to area X. These two neuron classes exhibit multiple neurochemical differences and are differentially replaced by new neurons during adult life: high rates of neuronal replacement are observed in RA-projecting neurons only. The activity of these two types of neurons may also be modulated differentially by steroids. We analyzed by magnetic resonance imaging the effect of testosterone on the volume of RA and area X and on the dynamics of Mn2+ accumulation in RA and area X of female starlings that had been injected with MnCl2 through a permanent cannula implanted in HVC. Repeated visualization 6 weeks apart (before and after testosterone treatment) identified a volume increase of both nuclei in testosterone-treated birds associated with a concomitant decrease in controls. Following testosterone treatment, the total amount of Mn2+ transported to RA and area X increased but the dynamics of accumulation, reflecting in part the activity of HVC neurons, was specifically altered in area X but not in RA. These data indicate that testosterone differentially affects the RA- and area X-projecting neurons in HVC. Manganese-enhanced magnetic resonance imaging (ME-MRI) thus provides repeated measures of connected brain areas and demonstrates testosterone-dependent regionally specific changes in brain activity and functional connectivity. The slow time scales investigated by this technique (compared to functional MRI) appear ideally suited for characterizing slow processes such as those involved in brain plasticity and learning. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 109 (11 ULg)
Full Text
Peer Reviewed
See detailLe cas clinique du mois. Syndrome de Down et myxoedeme: description d'un cas et revue de la litterature
Serpe, Philippe ULg; Gaillard, J. F.; Serpe, J. N. et al

in Revue Médicale de Liège (2004), 59(3), 131-135

A case of myxedema secondary to undetected hypothyroidism developed in a 48 year old 21-trisomic male. This paper presents a short discussion about different possible diagnoses and a review of the ... [more ▼]

A case of myxedema secondary to undetected hypothyroidism developed in a 48 year old 21-trisomic male. This paper presents a short discussion about different possible diagnoses and a review of the literature concerning the relationship between Down Syndrome and hypothyroidism or other diseases. [less ▲]

Detailed reference viewed: 126 (6 ULg)
Full Text
Peer Reviewed
See detailNon-O157 Shiga toxin-producing Escherichia coli isolated from diarrhoeic calves in Argentina.
Mercado, E. C.; Gioffre, A.; Rodriguez, Sabrina ULg et al

in Journal of Veterinary Medicine. B, Infectious Diseases & Veterinary Public Health (2004), 51(2), 82-8

Faecal samples from 76 diarrhoeic calves belonging to 36 farms located in the Pampas plain, Argentina, were examined for Shiga toxin-producing Escherichia coli (STEC). A total of 15 STEC strains were ... [more ▼]

Faecal samples from 76 diarrhoeic calves belonging to 36 farms located in the Pampas plain, Argentina, were examined for Shiga toxin-producing Escherichia coli (STEC). A total of 15 STEC strains were isolated from 12 (15.8%) calves which came from six different farms. All stx positive strains assayed by PCR were also positives in the Vero cell cytotoxicity test. The majority (60.0%) of the STEC strains carried the stx(1) gene. Twelve (80.0%) of the STEC isolates which belonged to serotypes O5:H- (n = 4), O26:H11 (n = 4), O26:H- (n = 1), O111:H- (n = 2), and O123:H38 (n = 1) were also enterohaemolysin (EHly) positive and carried the gene encoding for intimin (eae). All the stx positive strains were negative for the bfpA gene. Localized adherence to HEp-2 cells were observed in 83.3% of the eae+ STEC strains. STEC belonging to serotype O5:H- showed atypical biochemical properties, including urease production. Urease was also produced by two strains belonging to serotypes O153:H? and non-typeable, respectively. Resistance to three or more antibiotics was observed in 12 (80.0%) of the STEC isolates. Most of the serotypes of STEC recovered in this survey carried virulence traits that are associated with increased human and bovine pathogenicity. The present study shows that highly virulent STEC strains are being shed by diarrhoeic calves from farms located in a high incidence area of human STEC infections. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailAscorbate potentiates the cytotoxicity of menadione leading to an oxidative stress that kills cancer cells by a non-apoptotic caspase-3 independent form of cell death
Verrax, Julien; Cadrobbi, Julie; Marques, Carole et al

in Apoptosis : An International Journal on Programmed Cell Death (2004), 9(2), 223-233

Hepatocarcinoma cells (TLT) were incubated in the presence of ascorbate and menadione, either alone or in combination. Cell death was only observed when such compounds were added simultaneously, most ... [more ▼]

Hepatocarcinoma cells (TLT) were incubated in the presence of ascorbate and menadione, either alone or in combination. Cell death was only observed when such compounds were added simultaneously, most probably due to hydrogen peroxide (H2O2) generated by ascorbate-driven menadione redox cycling. TLT cells were particularly sensitive to such an oxidative stress due to its poor antioxidant status. DNA strand breaks were induced by this association but this process did not correspond to oligosomal DNA fragmentation (a hallmark of cell death by apoptosis). Neither caspase-3-like DEVDase activity, nor processing of procaspase-3 and cleavage of poly(ADP-ribose) polymerase (PARP) were observed in the presence of ascorbate and menadione. Cell death induced by such an association was actively dependent on protein phosphorylation since it was totally prevented by preincubating cells with sodium orthovanadate, a tyrosine phosphatase inhibitor. Finally, while H2O2, when administered as a bolus, strongly enhances a constitutive basal NF-kappaB activity in TLT cells, their incubation in the presence of ascorbate and menadione results in a total abolition of such a constitutive activity. [less ▲]

Detailed reference viewed: 68 (4 ULg)
Full Text
Peer Reviewed
See detailA new model for human intestinal preservation: Comparison of University of Wisconsin and Celsior preservation solutions
De Roover, Arnaud ULg; de Leval, Laurence ULg; Gilmaire, Julie ULg et al

in Transplantation Proceedings (2004), 36(2), 270-272

AIM: We compared University of Wisconsin (UW) and Celsior preservation solutions using a new model of human intestinal preservation that mimics the clinical conditions of small bowel procurement. METHODS ... [more ▼]

AIM: We compared University of Wisconsin (UW) and Celsior preservation solutions using a new model of human intestinal preservation that mimics the clinical conditions of small bowel procurement. METHODS: Intestinal grafts were harvested from four multiorgan donors. After classic warm dissection for organ procurement, an ileal segment of 50 cm was immediately flushed with Celsior. After the perfusion of the abdominal organs with UW, a second segment of adjacent ileum was harvested. The two intestinal grafts were then divided into segments by stapling, before immersion into the corresponding preservation solution (Celsior or UW) for 0-, 6-, 12-, or 24-hour incubation at 4 degrees C. A histological score was graded after blinded examination of three random specimens within each ileal graft for each duration of preservation. RESULTS: Control specimens showed normal histology. After 6 hours of preservation, most villi showed complete epithelial detachment although the crypts appeared intact. After 12 hours of preservation, a larger proportion of the villi showed extensive epithelial sloughing. After 24 hours, the damage involved the entire mucosa with the crypt epithelium largely detached from the basal membrane. No statistical difference in histological score was observed between the two preservation solutions. CONCLUSION: This study showed severe histological alterations of graft mucosa after short periods of preservation by UW or Celsior solutions. This model may be useful to evaluate improvements in the quality of preservation of human intestinal transplants. [less ▲]

Detailed reference viewed: 31 (3 ULg)
Full Text
Peer Reviewed
See detailInfluence of membrane-bound tumor necrosis factor (TNF)-alpha on obesity and glucose metabolism
Vörös, Gabor; Maquoi, Erik ULg; Collen, Désiré et al

in Journal of Thrombosis and Haemostasis (2004), 2(3), 507-513

OBJECTIVES: To investigate the influence of transmembrane tumor necrosis factor (TNF)-alpha on adipose tissue development and insulin-mediated glucose metabolism. METHODS AND RESULTS: TNF-alpha and ... [more ▼]

OBJECTIVES: To investigate the influence of transmembrane tumor necrosis factor (TNF)-alpha on adipose tissue development and insulin-mediated glucose metabolism. METHODS AND RESULTS: TNF-alpha and lymphotoxin-alpha-deficient mice expressing non-cleavable transmembrane TNF-alpha (Tg-tmTNF-alpha) and TNF-alpha/lymphotoxin-alpha double knockout (control) mice were kept on high-fat diet for 15 weeks. The food intake and feeding efficiency of Tg-tmTNF-alpha mice were significantly higher compared with control mice. At the end of the study, Tg-tmTNF-alpha mice had a significantly higher total body weight, as well as subcutaneous and gonadal adipose tissue mass. Histological analysis revealed that the expression of Tg-tmTNF-alpha resulted in a significantly increased adipocyte area and blood vessel density. Plasma leptin levels correlated positively with adipose tissue mass. The plasma levels of total cholesterol and HDL-cholesterol were significantly increased and LDL-cholesterol levels significantly decreased in Tg-tmTNF-alpha mice. Fasting blood glucose and plasma insulin levels were not different between the two genotypes and intraperitoneal glucose and insulin tolerance tests did not show significant differences. CONCLUSIONS: Transmembrane TNF-alpha enhances adipose tissue formation without altering insulin-mediated glucose metabolism in mice with nutritionally induced obesity. [less ▲]

Detailed reference viewed: 45 (1 ULg)
Full Text
Peer Reviewed
See detailNon-linear optical measurements and crystalline characterization of CdTe nanoparticles produces by thes electropulse technique
Loicq, Jerôme ULg; Renotte, Yvon ULg; Delplancke, Jean-Luc

in New Journal of Physics (2004), 6(32),

We propose to extend the ‘electropulse’ technique to synthesize CdTe nanoparticles for optical components. To do so, we create nuclei of the expected material on a titanium electrode immersed in an ... [more ▼]

We propose to extend the ‘electropulse’ technique to synthesize CdTe nanoparticles for optical components. To do so, we create nuclei of the expected material on a titanium electrode immersed in an electrolytic solution by using an electrochemical pulse. The nanoparticles are expelled from the titanium horn surface by cavitation bubbles, which are produced in the solution by a high intensity ultrasound pulse generated by a piezoelectric crystal. Optical absorption spectra and third-order non-linear optical properties of a colloidal solution of CdTe particles produced by this technique are presented. The non-linear refractive index was characterized using the single-beam z-scan technique, and measurements were carried out at several incidence intensities. Non-linear indices of refraction between −1.2×10−7 and −2×10−8 cm2W−1 were measured. [less ▲]

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailAromatization of androgens into estrogens reduces response latency to a noxious thermal stimulus in male quail
Evrard, H. C.; Balthazart, Jacques ULg

in Hormones & Behavior (2004), 45(3), 181-189

We recently demonstrated the presence of estrogen synthase (aromatase) and of estrogen receptors in the dorsal horn (laminae I-II) throughout the rostrocaudal extent of the spinal cord in male and female ... [more ▼]

We recently demonstrated the presence of estrogen synthase (aromatase) and of estrogen receptors in the dorsal horn (laminae I-II) throughout the rostrocaudal extent of the spinal cord in male and female Japanese quail. The spinal laminae I-II receive and process abundant sensory information elicited, among others, by acute noxious stimulation of the skin and resulting in rapid, reflex-like withdrawal behavior. In the present study, we demonstrate that systemic treatment with estradiol or testosterone markedly decreases the latency of the foot withdrawal in the hot water test. A simultaneous treatment with an aromatase inhibitor blocks the effects of testosterone demonstrating, hence, that they are mediated by a conversion of testosterone into an estrogen by aromatase. Furthermore, the testosterone- or estradiol-induced decrease in foot withdrawal latency is blocked by a treatment with the estradiol receptor antagonist, tamoxifen, indicating that the effects are largely mediated by the interaction of estradiol with estrogen receptors. Together, these data suggest that sex steroids modulate sensitivity to noxious stimuli possibly by a direct action at the level of the dorsal hom of the spinal cord. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 26 (0 ULg)
Full Text
Peer Reviewed
See detailNetworks of equities in financial markets
Bonanno, G.; Caldarelli, G.; Lillo, F. et al

in European Physical Journal B -- Condensed Matter (2004), 38(2), 363-371

We review the recent approach of correlation based networks of financial equities. We investigate portfolio of stocks at different time horizons, financial indices and volatility time series and we show ... [more ▼]

We review the recent approach of correlation based networks of financial equities. We investigate portfolio of stocks at different time horizons, financial indices and volatility time series and we show that meaningful economic information can be extracted from noise dressed correlation matrices. We show that the method can be used to falsify widespread market models by directly comparing the topological properties of networks of real and artificial markets. [less ▲]

Detailed reference viewed: 19 (0 ULg)
Full Text
Peer Reviewed
See detailWood formation in in vitro propagated walnut shoots in relation with root formation and development
Kevers, Claire ULg; Bisbis, Badia; Crèvecoeur, Michèle et al

in Acta Botanica Gallica : Bulletin de la Société Botanique de France (2004), 151(1), 45-53

Lignification and xylem cell multiplication for wood formation were examined in in vitro propagated walnut shoot cuttings after transfer on an auxin-containing rooting medium for one week and subsequently ... [more ▼]

Lignification and xylem cell multiplication for wood formation were examined in in vitro propagated walnut shoot cuttings after transfer on an auxin-containing rooting medium for one week and subsequently during root development in vermiculite in the absence of growth regulators. Lignification in the shoot stems started immediately after the exogenous auxin treatment which implied changes in peroxidase activity and in free IAA levels. Sustained lignification required the completion of the following rooting phases. The lignin was exclusively located in xylem cells, the number of which increased with the number of developing roots. The mutual interactions between the aerial parts of the plants and their roots are discussed. [less ▲]

Detailed reference viewed: 102 (3 ULg)
Full Text
Peer Reviewed
See detailL'image du mois. Un greffon hepatique pour deux receveurs : le foie partage ou split liver
Detry, Olivier ULg; De Roover, Arnaud ULg; Meurisse, Michel ULg et al

in Revue Médicale de Liège (2004), 59(3), 117

Detailed reference viewed: 41 (6 ULg)
Full Text
Peer Reviewed
See detailHomogenization of lamination stacks in linear magnetodynamics
Krahenbuhl, Laurent; Dular, Patrick ULg; Zeidan, Tarek et al

in IEEE Transactions on Magnetics (2004), 40(2, Part 2), 912-915

An effective numerical modeling of devices with lamination stacks cannot be done without replacing the laminations by an equivalent solid region. So far, no convincing model has been proposed, even in the ... [more ▼]

An effective numerical modeling of devices with lamination stacks cannot be done without replacing the laminations by an equivalent solid region. So far, no convincing model has been proposed, even in the "simple" case of linear magnetodynamics-particularly if the resultant current in each sheet does not cancel. In this paper, we define the homogenized fields, and the related material properties. The noteworthy precision of the theoretic homogenized solution is shown and discussed by comparing it with the exact solution of the problem with laminations in the case of a simple "1.5-D" analytical test problem. The three-dimensional finite element solutions-real description of the sheets, refined mesh/homogenized region, coarser mesh-for the same test problem show also a perfect agreement. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
See detailLe traitement de l’obésité chez le chien
Diez, Marianne ULg; Jeusette, Isabelle; Nguyen, Patrick

in Le nouveau praticien vétérinaire (2004)

Detailed reference viewed: 18 (4 ULg)
Full Text
Peer Reviewed
See detailSocial support and health-related quality of life in hip and knee osteoarthritis
Ethgen, Olivier ULg; Vanparijs, Philippe ULg; Delhalle, S. et al

in Quality of Life Research (2004), 13(2), 321-330

Objective: To document the association between social support and health-related quality of life (HRQoL) in hip and knee osteoarthritis (OA). Methods: A prospective survey including the SF-36 and the ... [more ▼]

Objective: To document the association between social support and health-related quality of life (HRQoL) in hip and knee osteoarthritis (OA). Methods: A prospective survey including the SF-36 and the Social Support questionnaire (SSQ) was administered to 108 hip and knee OA patients attending an outpatient physical rehabilitation and rheumatology clinic. Multiple regression analysis were performed to study the relation between social support and each dimension of the SF-36, controlling for age, sex, body mass index, number of comorbid conditions, socioeconomic status, site of survey completion and severity of OA which was gauged with the pain dimension of the WOMAC, an OA-specific health status instrument. Results: Greater social companionship transactions were associated with higher physical functioning ( standardized regression coefficients: beta = 0.26, p < 0.01), general health (β = 0.32, p < 0.001), mental health (beta = 0.25, p < 0.01), social functioning (β = 0.20, p < 0.05) and vitality ( b = 0.25, p < 0.05). Satisfaction with problem-oriented emotional support was related to better physical functioning (β = 0.22, p < 0.01), mental health (beta = 0.38, p < 0.001), role-emotional (B = 0.23, p < 0.01), social functioning (beta = 0.19, p < 0.05) and vitality ( b = 0.26, p < 0.01). Conclusion: Social support components significantly account for HRQoL. Health interventions in OA, primary dedicated to pain and physical disability, could be supplemented with social support component to enhance health outcomes. [less ▲]

Detailed reference viewed: 45 (4 ULg)
Full Text
Peer Reviewed
See detailComment je traite ... la mucite bucco-pharyngee lors d'une radiotherapie cervico-faciale
Piret, Pascal ULg; Deneufbourg, Jean-Marie ULg

in Revue Médicale de Liège (2004), 59(3), 120-7

Oral mucositis the major acute effect of head and neck radiotherapy. According to the degree, irradiation may be compromised in dose and overall time with consequences on outcome. Up to now there is no ... [more ▼]

Oral mucositis the major acute effect of head and neck radiotherapy. According to the degree, irradiation may be compromised in dose and overall time with consequences on outcome. Up to now there is no standard accepted protocol. A strict program of local and general hygiene is mandatory. Analgesics are often required. Amifostine acts as a protector of salivary glands GM-CSF and G-CSF stimulate proliferation of mucosal basal cells. Sucralfate plays a barrier role. Camomile plant extract is anti-inflammatory, well tolerated and cheap. Povidone-iodine is better than chlorhexidine as an antiseptic agent. PTA lozenges with polymixin E, tobramycin and amphotericin B showed a marked power of selective microbial decontamination. [less ▲]

Detailed reference viewed: 494 (9 ULg)
Full Text
Peer Reviewed
See detailAn XMM-Newton observation of the massive binary HD 159176
De Becker, Michaël ULg; Rauw, Grégor ULg; Pittard, J. M. et al

in Astronomy and Astrophysics (2004), 416(1), 221-233

We report the analysis of an XMM-Newton observation of the close binary HD 159176 (O7 V + O7 V). The observed L-x/L-bol ratio reveals an X-ray luminosity exceeding by a factor similar to7 the expected ... [more ▼]

We report the analysis of an XMM-Newton observation of the close binary HD 159176 (O7 V + O7 V). The observed L-x/L-bol ratio reveals an X-ray luminosity exceeding by a factor similar to7 the expected value for X-ray emission from single O-stars. therefore suggesting a wind-wind interaction scenario. EPIC and RGS spectra are fitted consistently with a two temperature mekal optically thin thermal plasma model, with temperatures ranging from similar to2 to 6 x 10(6) K. At first sight, these rather low temperatures are consistent with the expectations for a close binary system where the winds collide well before reaching their terminal velocities. We also investigate the variability of the X-ray light curve of HD,159176 on various short time scales. No significant variability is found and we-conclude that if hydrodynamical instabilities exist in the wind interaction region of HD 159176, they are not sufficient to produce an observable signature in the X-ray emission. Hydrodynamic simulations using wind parameters from the literature reveal some puzzling discrepancies. The most-striking one concerns the predicted X-ray luminosity which is one or more orders of magnitude larger than the observed one. A significant reduction of the mass loss rate of the components compared to the values quoted in the literature alleviates the discrepancy but is not sufficient to fully account for the observed luminosity. Because hydrodynamical models are best for the adiabatic case whereas the colliding winds in HD 159176 are most likely highly radiative, a totally new approach has been envisaged, using a geometrical steady-state colliding wind model suitable for the case of radiative winds. This model successfully reproduces the spectral shape of the EPIC spectrum, but further developments are still needed to alleviate the disagreement between theoretical and observed X-ray luminosities. [less ▲]

Detailed reference viewed: 23 (4 ULg)