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See detailIsolation, identification and serotyping of Clostridium perfringens from goats.
Phukan, A.; Dutta, G. N.; Daube, Georges ULg et al

in Indian Journal of Animal Sciences (The) (1997), 67

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See detailHepatocyte transplantation in anhepatic rats: effect on survival, blood chemistry and growth factor profile
Khalili, T.; Arkadopoulos, N.; Detry, Olivier ULg et al

in Surgical Forum (1997), 44

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See detailImproved emulsifying and foaming of whey proteins after enzymic fat hydrolysis
Blecker, Christophe ULg; Paquot, Michel ULg; Lamberti, I. et al

in Journal of Food Science (1997), 62(1), 48-

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This ... [more ▼]

In an effort to improve emulsifying and foaming of sweet whey and whey protein concentrate, we studied the hydrolysis of the residual fat in these preparations with a sn 1,3 specific lipase. This enzymatic lipolysis would convert almost all triglycerides into mono- and diglycerides which have strong surface-active properties. Therefore, the emulsifying and foaming capacities of such modified whey products should be increased in comparison with native products, as shown by results presented at both neutral pH and those similar to a salad dressing emulsion. It was also demonstrated that emulsions and foams formed with lipase-treated whey preparations were more shelf-stable. [less ▲]

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See detailHair follicle involvement in herpes zoster
Nikkels, Arjen ULg; Pierard, Gérald ULg

in Virchows Archiv : An International Journal of Pathology (1997), 430

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See detailEnhancement of Tamoxifen-Induced E-Cadherin Function by Ca2+ Channel Antagonists in Human Breast Cancer Mcf7/6 Cells
Charlier, Corinne ULg; Bruyneel, E.; Lechanteur, Chantal ULg et al

in European Journal of Pharmacology (1996), 317(2-3), 413-6

Despite its intensive use in adjuvant breast cancer therapy for more than 30 years, the exact mechanisms of action of tamoxifen have not yet been fully characterized. Tamoxifen was recently shown to ... [more ▼]

Despite its intensive use in adjuvant breast cancer therapy for more than 30 years, the exact mechanisms of action of tamoxifen have not yet been fully characterized. Tamoxifen was recently shown to restore the E-cadherin function of human breast cancer MCF7/6 cells and to suppress their invasive phenotype. Because tamoxifen interacts with targets implicated in Ca2+ homeostasis, we explored the possibility that the restoration of E-cadherin function in MCF7/6 cells induced by this drug could be affected by Ca2+ modulators. Two different Ca2+ channel antagonists (verapamil and nifedipine) potentiated the effect of tamoxifen on E-cadherin function, as evaluated with a fast cell aggregation assay. These molecules decreased the tamoxifen concentration needed to restore the E-cadherin function from 10(-6) M to 10(-7) M. When incubated with a Ca2+ channel agonist, Bay K8644 (methyl-1,4-dihydro-2,6-dimethyl-3-nitro-4-(2-trifluoro-methylphenyl)- pyridine-5-carboxylate), the effect of tamoxifen on E-cadherin function was completely abolished. These results demonstrate that the restoration of the E-cadherin function induced by tamoxifen depends, at least in part, on a Ca2+ pathway, and support the evidence of an effect of tamoxifen on Ca(2+)-dependent mechanisms. Our data also suggest that Ca2+ channel modulators could make it possible to decrease the dose of tamoxifen administered to patients without reducing the therapeutic effects. [less ▲]

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See detailPoly[alkyl methacrylate-b-butadiene-b-alkyl methacrylate] triblock copolymers : synthesis, morphology, and mechanical properties at high temperatures
Yu, Jian Ming; Teyssié, Philippe; Jérôme, Robert ULg

in Macromolecules (1996), 29(26), 8362-8370

Block copolymers of the ABA type, where B is polybutadiene (PBD) and A is poly(ethyl methacrylate) (PEMA), poly(tert-butyl methacrylate) (P-t-BMA), poly(methyl methacrylate) (PMMA), or poly(isobornyl ... [more ▼]

Block copolymers of the ABA type, where B is polybutadiene (PBD) and A is poly(ethyl methacrylate) (PEMA), poly(tert-butyl methacrylate) (P-t-BMA), poly(methyl methacrylate) (PMMA), or poly(isobornyl methacrylate) (PIBMA), have been successfully synthesized by sequential anionic polymerization of butadiene and methacrylates with the diadduct of tert-butyllithium to m-diisopropenylbenzene as a difunctional initiator. Block copolymers of a narrow molecular weight distribution (1.10) have been analyzed by differential scanning calorimetry, transmission electron microscopy, and dynamic mechanical analysis. These materials are phase-separated and have high mechanical performances. Special attention has been paid to the service temperature of these thermoplastic elastomers in comparison with a styrene−butadiene−styrene (SBS) triblock copolymer. The upper service temperature (UST) has been estimated from the temperature dependence of the tensile properties in the 25−150 °C range and found to change with the outer blocks. Polystyrene (Tg = 100°C) is at the origin of the lower UST, which is however comparable to PEMA (Tg = 90 °C) and P-t-BMA (Tg = 116 °C) containing triblock copolymers of similar molecular weight and composition. PMMA (Tg = 132 °C) outer blocks increase the UST of the triblocks, which is further increased by hydrogenation of the PBD midblock. The higher UST has been found for a PIBMA (Tg = 202 °C)-containing triblock copolymer that shows an ultimate tensile strength higher than 2 MPa at 150 °C. [less ▲]

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See detailTriblock copolymer based thermoreversible gels. 3. Effect of stereocomplexation with iPMMA
Yu, Jian Ming; Jérôme, Robert ULg

in Macromolecules (1996), 29(26), 8371-9378

Thermoreversible gelation of syndiotactic poly(methyl methacrylate) (sPMMA)−polybutadiene (PBD)−sPMMA, triblock copolymers in o-xylene has been studied in the presence of isotactic PMMA (iPMMA) in a large ... [more ▼]

Thermoreversible gelation of syndiotactic poly(methyl methacrylate) (sPMMA)−polybutadiene (PBD)−sPMMA, triblock copolymers in o-xylene has been studied in the presence of isotactic PMMA (iPMMA) in a large range of syndio/iso mixing ratio (s/i). Differential scanning calorimetry (DSC) shows that two types of association contribute to the gel formation, i.e., the self-aggregation of the sPMMA outer blocks and the stereocomplexation of the sPMMA outer blocks with iPMMA. At low iPMMA contents (s/i = 30/1), self-association of the outer blocks accounts for the gelation process, although stereocomplexation occurs. Stereocomplexation becomes the dominant gelation mechanism at a high enough iPMMA content (s/i = 2/1). Thus, gelation is observed at a copolymer concentration as low as 1 wt %. The time dependence of the storage (G‘) and loss (G‘‘) moduli has been measured in the 0.08−1Hz frequency range in order to probe the gelation process. At the gel point, where the loss angle (tan δc = G‘‘/G‘) is independent of the probing frequency, the sample fits in the typical power law G‘(ω) G‘‘(ω) ωΔ. While the gelation time is greatly influenced by the s/i mixing ratio, the scaling exponent Δ is essentially constant in the range of 0.70−0.75. The Δ value agrees with theoretical predictions as well as with experimental values reported for some chemical gels but it is different from the experimental values reported for most physical gels. The Δ value is also independent of the PBD microstructure and it also holds for sPMMA-polystyrene (PS)−PBD−PS−sPMMA (MSBSM) pentablock copolymers. Therefore, the value of Δ appears to be a typical exponent for thermoplastic gels based on triblock copolymers of the ABA type in o-xylene. [less ▲]

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See detailIdentification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein.
Clausse, Nathalie; Jackers, Pascale ULg; Jares, P. et al

in DNA & Cell Biology (1996), 15(12), 1009-23

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents ... [more ▼]

A 37LRP/p40 polypeptide is of major interest because it is consistently up-regulated in cancer cells in correlation with their invasive and metastatic phenotype. Furthermore, this polypeptide presents intriguing multifunctional properties because it has been characterized as the precursor of the metastasis-associated 67-kD laminin receptor (67LR) and as a cytoplasmic ribosomal-associated protein. The isolation of the 37LRP/p40 gene is a prerequisite for identifying the molecular mechanisms responsible for the constant up-regulation of the 67LR expression in cancer cells. To date, the active 37LRP/p40 gene has never been identified in any species due to the existence of multiple pseudogenes in most vertebrates genomes. In this study, we report for the first time the gene structure and potential regulatory sequences of the 37 LRP/p40 gene. The chicken genome was selected to undergo this characterization because it is the only known vertebrate that bears a single 37 LRP/p40 gene copy. The 37 LRP/p40 active gene is composed of 7 exons and 6 introns and bears features characteristic of a ribosomal protein gene. It does not bear a classical TATA box and it exhibits several transcription initiation sites as demonstrated by RNase protection assay and primer extension. Analysis of potential regulatory regions suggests that gene expression is driven not only by the 5' genomic region but also by the 5' untranslated and intron 1 sequences. On the basis of gene structure and extensive protein evolutionary study, we found that the carboxyterminal domain of the protein is a conserved lock-and-key structure/function domain that could be involved in the biosynthesis of the higher-molecular-weight 67-kD laminin receptor in vertebrates, whereas the central core of the protein would be responsible for the ribosome associated function. The first identification of the active 37LRP/p40 gene presented in this study is a critical step toward the isolation of the corresponding human gene and the understanding of the molecular mechanisms involved in the up-regulation of its expression during tumor invasion and metastasis. [less ▲]

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See detailInterleukin-1 Beta Induces Nuclear Factor Kappa B in Epithelial Cells Independently of the Production of Reactive Oxygen Intermediates
Bonizzi, Giuseppina; Dejardin, Emmanuel ULg; Piret, Bernard et al

in European Journal of Biochemistry (1996), 242(3), 544-9

A large body of work has been devoted to tumor necrosis factor alpha or interleukin-1 beta (IL-1 beta) signaling leading to the activation of the transcription factor nuclear factor-kappa B (NF-kappa B ... [more ▼]

A large body of work has been devoted to tumor necrosis factor alpha or interleukin-1 beta (IL-1 beta) signaling leading to the activation of the transcription factor nuclear factor-kappa B (NF-kappa B) in various cell types. Several studies have indicated that NF-kappa B activation depends strictly on the production of reactive oxygen intermediates. In this report, we first demonstrated that IL-1 beta is a potent activator of NF-kappa B in various epithelial transformed cell lines (OVCAR-3, SKOV-3, MCF7 A/Z). In these cells, IL-1 beta rapidly induces NF-kappa B through a complete degradation of I kappa B-alpha, while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105. We showed that IL-1 beta-mediated induction of NF-kappa B in OVCAR-3 and in other epithelial cell lines does not proceed through the production of reactive oxygen intermediates, while the same cytokine activates NF-kappa B in lymphoid cells through the intracellular generation of H2O2. Our study demonstrated that several signaling pathways lead to the activation of NF-kappa B, following IL-1 beta treatment in different cell types. [less ▲]

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See detailReduction in Requirements for Allogeneic Blood Products: Pharmacologic Methods
Janssens, Marc ULg; Hartstein, Gary ULg; David, Jean-Louis ULg

in Annals of Thoracic Surgery (1996), 62(6), 1944-50

BACKGROUND: Numerous articles describe the reduction of perioperative bleeding by the therapeutic or prophylactic administration of drugs such as prostacyclin, desmopressin, and natural or synthetic ... [more ▼]

BACKGROUND: Numerous articles describe the reduction of perioperative bleeding by the therapeutic or prophylactic administration of drugs such as prostacyclin, desmopressin, and natural or synthetic antifibrinolytics. METHODS: A review of the literature was carried out to help the reader define the indications of these drugs during cardiopulmonary bypass operations, highlight the questions that remain concerning their indications and modes of action, and suggest future studies to answer these remaining questions. RESULTS: Prostacyclin reduces platelet trauma induced by extracorporeal circulation but does not effectively reduce postoperative bleeding and transfusion requirements. Desmopressin acts as a "glue," improving platelet adhesion, and may be effective when postoperative bleeding is excessive, but its routine use in cardiac operations cannot be recommended. Natural and synthetic antifibrinolytics inhibit plasmin and plasmin-induced platelet dysfunction. These agents have been shown to decrease bleeding and the need for allogeneic transfusions after open heart operations. However, with antifibrinolytic drugs, the risk of thromboembolic phenomena cannot be neglected. With aprotinin, this risk appears to be minimal when the drug is used at concentrations high enough to inhibit plasma kallikrein also. CONCLUSIONS: Prophylactic antifibrinolytics are efficacious, but their routine use remains controversial, both for economic reasons and for fear of thromboembolic complications. [less ▲]

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See detailAnionic polymerization of various methacrylates initiated with LiCl-complexed sBuLi
Antoun, S.; Wang, Jin-Shan; Jérôme, Robert ULg et al

in Polymer (1996), 37(25), 5755-5759

A simple alkyl lithium initiator (sBuLi) complexed with LiCl (10 molar equiv.) has been used to initiate the anionic polymerization of various methacrylic monomers, i.e. methyl methacrylate (MMA), tert ... [more ▼]

A simple alkyl lithium initiator (sBuLi) complexed with LiCl (10 molar equiv.) has been used to initiate the anionic polymerization of various methacrylic monomers, i.e. methyl methacrylate (MMA), tert-butyl methacrylate (tBuMA), glycidyl methacrylate (GMA), and dimethyl amino ethyl methacrylate (DMAEMA) in tetrahydrofuran at -78°C. Only the homopolymerization of tBuMA proceeds in a living manner, as evidenced by both the linear plot of experimental molecular weight (Mnexp) vs theoretical ones (Mncal,) (slope % 0.93) and monomer resumption experiments. However, three types of block copolymers, i.e. PtBuMA-b-PMMA, PtBuMA-b-PGMA, PtBuMA-b-PDMAEMA, have been successfully synthesized with a predictable molecular weight, and narrow molecular weight distribution. [less ▲]

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See detailStaging of the Mediastinum: Value of Positron Emission Tomography Imaging in Non-Small Cell Lung Cancer
Bury, Thierry ULg; Paulus, P.; Dowlati, A. et al

in European Respiratory Journal : Official Journal of the European Society for Clinical Respiratory Physiology (1996), 9(12), 2560-4

Recent studies have shown limitations of morphological imaging in staging mediastinal lymph node involvement in lung cancer. In contrast to computed tomography (CT), which depends primarily on anatomical ... [more ▼]

Recent studies have shown limitations of morphological imaging in staging mediastinal lymph node involvement in lung cancer. In contrast to computed tomography (CT), which depends primarily on anatomical imaging features, positron emission tomography (PET) with 18-fluorodeoxyglucose (FDG) depends mainly on the metabolic characteristics of a tissue for the diagnosis of disease. We have performed a prospective study comparing FDG-PET and CT of the thorax in the presurgical assessment of the mediastinum in 50 patients with newly diagnosed non-small cell lung cancer (NSCLC). CT and PET scans were interpreted separately, and results were compared to pathological staging obtained during thoracotomy. Hilar or mediastinal lymph node involvement was present in 58%. In staging for lymph node involvement, CT had a sensitivity of 72% and specificity of 81%, whereas PET had a sensitivity and specificity of 90% and 86%, respectively. When the PET study was compared to histological results, there were four cases showing more advanced mediastinal involvement with PET and four cases showing less involvement with PET. From our preliminary results, we conclude that positron emission tomography with 18-fluorodeoxyglucose is significantly more accurate than computed tomography in the mediastinal staging of non-small cell lung cancer. [less ▲]

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See detailLa CEI entre mythes et réalités
Bachkatov, Nina ULg

in Monde Diplomatique (Le) : Journal des Cercles Diplomatiques et des Grandes Organisations Internationales (1996)

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See detailTnp-470 (Agm-1470): Mechanisms of Action and Early Clinical Development
Castronovo, Vincenzo ULg; Belotti, D.

in European Journal of Cancer (1996), 32A(14), 2520-7

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See detailOncological Applications of Positron Emission Tomography with Fluorine-18 Fluorodeoxyglucose
Rigo, Pierre ULg; Paulus, Patrick; Kaschten, Bruno et al

in European Journal of Nuclear Medicine (1996), 23(12), 1641-74

Positron emission tomography (PET) is now primarily used in oncological indication owing to the successful application of fluorine-18 fluorodeoxyglucose (FDG) in an increasing number of clinical ... [more ▼]

Positron emission tomography (PET) is now primarily used in oncological indication owing to the successful application of fluorine-18 fluorodeoxyglucose (FDG) in an increasing number of clinical indications at different stages of diagnosis, and for staging and follow-up. This review first considers the biological characteristics of FDG and then discusses methodological considerations regarding its use. Clinical indications are considered, and the results achieved in respect of various organs and tumour types are reviewed in depth. The review concludes with a brief consideration of the ways in which clinical PET might be improved. [less ▲]

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See detailScreening and characterization of specific anti-lipopolysaccharide antibodies in Belgian blood donors by enzyme-linked immunosorbent assays
Nys, Monique ULg; Laub, R.; Damas, Pierre ULg et al

in European Journal of Clinical Investigation (1996), 26(12), 1134-1142

The goal of this project was to find and collect high concentrations of endotoxin-specific antibodies for therapeutic IgG- or IgM-enriched preparations. Various enzyme-linked immunosorbent assays (ELISAs ... [more ▼]

The goal of this project was to find and collect high concentrations of endotoxin-specific antibodies for therapeutic IgG- or IgM-enriched preparations. Various enzyme-linked immunosorbent assays (ELISAs) were developed to perform longitudinal studies of the serological response to a large panel of smooth and rough purified lipopolysaccharide (LPS) extracts in a population of healthy blood donors. To accomplish this, 1612 human serum samples from volunteer blood donors collected by seven different blood banks in Belgium were screened and specific IgM and IgG activities were measured. Approximately 17% of the donors had anti-LPS concentrations higher than 40 mg L-1. Of these, 10.9% had anti-smooth LPS antibodies, 3.7% had anti-rough LPS antibodies and 2.8% were found to be positive towards both types of LPS. The mean anti-LPS antibody concentration was 8 mg L-1 for rough LPS and 14 mg L-1 for smooth LPS. Age- and sex-related distributions of the activities indicated that the greatest prevalence of high anti-LPS concentration was in women aged 40-49 years and in men older than 60 years. Differential absorption experiments showed that the pooled serum of selected blood donors contained a mixture of specific and cross-reacting antibodies. We detected predominantly anti-LPS activities due to the IgG1 and IgG2 subclasses. The range of specificities to different LPS was increased by the pooling of selected sera. It was concluded that pools of naturally occurring specific anti-LPS immunoglobulin antibodies may be obtained in Belgium by screening blood donors using ELISAs that we have developed. [less ▲]

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See detailMicrostructure development in isothermally melt-textured 123-211 composite materials
Auguste, F.; Rulmont, André ULg; Vandewalle, Nicolas ULg et al

in Journal of Low Temperature Physics (1996), 105(5-6), 1439-1444

Microstructures of isothermally melt-textured 123-211 composite materials have been examined during the solidification process from quenched specimens. The distribution of 211 particles, the ... [more ▼]

Microstructures of isothermally melt-textured 123-211 composite materials have been examined during the solidification process from quenched specimens. The distribution of 211 particles, the superconducting 123 grain boundary and the solid-liquid interface features are emphasized. The quality of the materials has been investigated by thermopower and thermal conductivity measurements. A seeded-melt texturing technique has been also tested in order to grow large single-domain of DyBa2Cu3O7-y. The chosen seed was a Dy2O3 single crystal. [less ▲]

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See detailDo Sex Differences in the Brain Explain Sex Differences in the Hormonal Induction of Reproductive Behavior? What 25 Years of Research on the Japanese Quail Tells Us
Balthazart, Jacques ULg; Tlemcani, O.; Ball, G. F.

in Hormones & Behavior (1996), 30(4), 627-61

Early workers interested in the mechanisms mediating sex differences in morphology and behavior assumed that differences in behavior that are commonly observed between males and females result from the ... [more ▼]

Early workers interested in the mechanisms mediating sex differences in morphology and behavior assumed that differences in behavior that are commonly observed between males and females result from the sex specificity of androgens and estrogens. [less ▲]

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See detailGliomatosis Cerebri: Clinical, Radiological and Pathological Report of a Case with a Stroke-Like Onset
Schoenen, Jean ULg; de Leval, L.; Reznik, M.

in Acta Neurologica Belgica (1996), 96(4), 294-300

A 62 year-old man was admitted with a right hemiparesis, sensory aphasia and right hemianopia which appeared on awakening. He was initially thought to have a stroke, but EEG showed diffuse slowing and ... [more ▼]

A 62 year-old man was admitted with a right hemiparesis, sensory aphasia and right hemianopia which appeared on awakening. He was initially thought to have a stroke, but EEG showed diffuse slowing and both CT scan and MRI irregular white matter lesion suggesting a leucoencephalopathy. His neurological deficit regressed, and he was discharged after 2 weeks. He was readmitted 6 months later because of mental confusion. MRI revealed diffuse white matter lesions extending up to the frontal lobes, these were hyperintense on T2 weighted images and suggested the diagnosis of gliomatosis cerebri (GC). The patient became progressively comatose and died 6 weeks later. At autopsy the brain looked diffusely swollen with irregular greyish areas of the white matter of both centrum ovale and brain stem. On microscopic examination the cerebrum and brain stem were diffusely and asymmetrically infiltrated by numerous neoplastic glial cells without angiogenesis or disruption of architectonic boundaries. There were no mitoses nor necrosis. Many tumour cells were GFAP- and S100-positive. A high proportion of cells contained the leucocyte antigen Leu-7. This case of gliomatosis cerebri is compared to the 9 published cases of GC with an initial focal neurological deficit and to the 19 publications reporting MRI results. The controversial nosological boundaries and etiopathogenetic hypotheses of this peculiar neoplastic disease are discussed. [less ▲]

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See detailTreatment of Excessive Mediastinal Bleeding after Cardiopulmonary Bypass
Hartstein, Gary ULg; Janssens, Marc ULg

in Annals of Thoracic Surgery (1996), 62(6), 1951-4

BACKGROUND: Excessive mediastinal bleeding after cardiopulmonary bypass is one of the most frequently reported complications of cardiac operations. Appropriate treatment requires a rapid and effective ... [more ▼]

BACKGROUND: Excessive mediastinal bleeding after cardiopulmonary bypass is one of the most frequently reported complications of cardiac operations. Appropriate treatment requires a rapid and effective diagnostic work-up, based on the knowledge of the pathophysiology induced by cardiopulmonary bypass. METHODS: Possible causes, diagnostic methods available, and therapeutic approaches are reviewed in the light of the literature published on excessive bleeding after cardiac operations. RESULTS: When bleeding is massive (> 250 to 300 mL/h for the first 2 hours, > 150 mL/h thereafter), immediate surgical reexploration is mandatory. When bleeding is less important (50 to 150 mL/h), the decision to reoperate should be based on the presence of hemodynamic compromise or a suspected surgical cause. Otherwise, coagulation testing should allow the correction of hemostatic defects as appropriate with protamine, platelet concentrates, fresh frozen plasma, desmopressin, or antifibrinolytics. Hypothermia and hypotension should be corrected and a trial of positive end-expiratory pressure may be considered if diffuse mediastinal oozing (especially from the bed of the mammary artery) is suspected. CONCLUSIONS: A protocol is suggested to guide treatment, taking into account the rapidity of blood loss and the suspected underlying cause. [less ▲]

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See detailAdaptation de la méthodologie des langues étrangères aux styles d'apprentissage des élèves : un nouveau miroir aux alouettes ?
Simons, Germain ULg

in Etudes et Travaux (1996), (1), 19-38

Selon certains spécialistes de l'apprentissage d'une langue étrangère, le style d'apprentissage d'un individu fonctionne comme un "filtre à input" et exerce, de ce fait, une influence considérable sur le ... [more ▼]

Selon certains spécialistes de l'apprentissage d'une langue étrangère, le style d'apprentissage d'un individu fonctionne comme un "filtre à input" et exerce, de ce fait, une influence considérable sur le processus d'apprentissage de la langue étrangère et sur le niveau de performance atteint par les différents types d'apprenants. Cette conception des styles d'apprentissage conduit naturellement à supposer que l'enseignement optimal d'une langue étrangère est celui dont la méthodologie est en adéquation avec le style d'apprentissage des élèves. Selon G. Simons, il convient d'aborder ce type de recherche avec la plus grande prudence si on ne veut pas qu'elle devienne un miroir aux alouettes, un de plus. Pour éviter ce piège, G. Simons analyse quelques problèmes sous-jacents à ce domaine de recherche et tente d'y apporter des solutions. [less ▲]

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See detailBeta-Carbolines Induce Apoptotic Death of Cerebellar Granule Neurones in Culture
Malgrange, Brigitte ULg; Rigo, Jean-Marie ULg; Coucke, Paul et al

in Neuroreport (1996), 7(18), 3041-5

Apart from its role in fast inhibitory transmission, only neurotrophic effects have been reported following activation of the GABAA receptor. Here, we show that n-butyl-beta-carboline-3-carboxylate and n ... [more ▼]

Apart from its role in fast inhibitory transmission, only neurotrophic effects have been reported following activation of the GABAA receptor. Here, we show that n-butyl-beta-carboline-3-carboxylate and n-methyl-beta-carboline-3-carboxamide, which are negative allosteric modulators of the GABAA receptor acting at the benzodiazepine site, are neurotoxic for cerebellar granule neurones in culture. The beta-carboline-induced neuronal death is apoptotic since DNA internucleosomal fragmentation was induced and the neurotoxicity could be prevented by inhibitors of mRNA or protein synthesis. As GABA and benzodiazepine ligands (diazepam and Ro 15-1788) protect cerebellar granule cells against beta-carboline-induced toxicity, these data raise the possibility that the interaction between the beta-carbolines and the GABAA receptor is the triggering event leading to neuronal apoptosis. [less ▲]

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See detailGrowth of Cayley and diluted Cayley trees with two kinds of entities
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Journal of Physics : A Mathematical & General (1996), 29(22), 7089-7104

A kinetic growth model derived from the magnetic Eden model is introduced in order to simulate the growth of hierarchical structures, such as Cayley trees. We only consider the case where two kinds of ... [more ▼]

A kinetic growth model derived from the magnetic Eden model is introduced in order to simulate the growth of hierarchical structures, such as Cayley trees. We only consider the case where two kinds of entities are competing with each other can be further subjected to an external field. The very relevant case in which both kinds of entities have different coordination numbers is introduced here for the first time, and is called the diluted Cayley tree. Physical and geometrical properties of the finite and infinite trees are exactly found and simulated. Finite-size effects are emphasized and illustrated on the global or local magnetization and on the chemical activity. Asymptotic limits are given in each case. The generated patterns can be related to a correlated percolation problem briefly discussed in the appendix. [less ▲]

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See detailDecrease of Plasma Vitamin E (Alpha-Tocopherol) Levels in Patients with Abdominal Aortic Aneurysm
Sakalihasan, Natzi ULg; Pincemail, Joël ULg; Defraigne, Jean-Olivier ULg et al

in Annals of the New York Academy of Sciences (1996), 800

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See detailCytokine Production by Human Thymic Epithelial Cells: Control by the Immune Recognition of the Neurohypophysial Self-Antigen
Martens, Henri ULg; Malgrange, Brigitte ULg; Robert, F. et al

in Regulatory Peptides (1996), 67(1), 39-45

Oxytocin (OT) has been shown to be the dominant peptide of the neurohypophysial family expressed by thymic epithelial and nurse cells (TEC/TNC) in various species. Thymic OT is not secreted but, after ... [more ▼]

Oxytocin (OT) has been shown to be the dominant peptide of the neurohypophysial family expressed by thymic epithelial and nurse cells (TEC/TNC) in various species. Thymic OT is not secreted but, after translocation of a hybrid neurophysin/MHC class I protein, is integrated within the plasma membrane of TEC, thus allowing its presentation to pre-T cells. In order to further demonstrate that thymic OT behaves like a membrane antigen, we assessed the effect of mAbs to OT on cytokine productions by cultures enriched in human TEC. 75-85% pure TEC cultures were prepared from human thymic fragments. Using immunofluorescence and confocal microscopy, ir-OT, ir-interleukin-1 beta (IL-1 beta), ir-interleukin-6 (IL-6) and ir-leukemia inhibitory factor (LIF) could be detected in these TEC cultures. ir-OT was restricted to TEC, while some ir-IL-6 and ir-LIF were also seen in occasional fibroblasts. In basal conditions, ir-IL-6 and ir-LIF (but not ir-OT and ir-IL-1 beta) were detected in the supernatants of human TEC cultures. MAbs to OT induced a marked increase of ir-IL-6 and ir-LIF secretion in TEC cultures. No significant effect was observed using mAbs against vasopressin, mouse immunoglobulins, or control ascitic fluid controls. These data show that OT is fully processed and recognized by specific mAbs at the outer surface of TEC plasma membrane. They further support that thymic OT behaves as the self-antigen of the neurohypophysial family. [less ▲]

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See detailTrypsin and Trypsinogen from an Antarctic Fish: Molecular Basis of Cold Adaptation
Genicot, S.; Rentier-Delrue, Françoise ULg; Edwards, D. et al

in Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology (1996), 1298(1), 45-57

Trypsin from Antarctic fish Paranotothenia magellanica displays molecular and kinetic properties typical of enzymes produced by psychrophilic organisms. The enzyme has a high catalytic efficiency at low ... [more ▼]

Trypsin from Antarctic fish Paranotothenia magellanica displays molecular and kinetic properties typical of enzymes produced by psychrophilic organisms. The enzyme has a high catalytic efficiency at low and moderate temperatures and is rapidly inactivated at temperatures higher than 30 degrees C. The nucleotide sequence was determined after mRNA extraction and cDNA synthesis. The cDNA encodes a pretrypsinogen which includes a seven residue activation peptide containing only three acidic residues preceeding the 222 amino-acid mature enzyme. A three-dimensional model of the enzyme was built. Structural parameters possibly involved in the adaptation to cold have been derived from comparison with the three-dimensional structure of the bovine enzyme. Among them are the lack of Tyr-151 in the substrate binding pocket, an overall decrease in the number of salt bridges and hydrophobicity and the increase in the surface hydrophilicity. [less ▲]

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See detailEvidence of involvement of yeast proliferating cell nuclear antigen in DNA mismatch repair
Johnson, Robert; Kovvali, G.; Guzder, Sami et al

in Journal of Biological Chemistry (1996), 271(45), 27897-90

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See detailProtein Kinase- and Staurosporine-Dependent Induction of Neurite Outgrowth and Plasminogen Activator Activity in Pc12 Cells
Leprince, Pierre ULg; Bonvoisin, Catherine ULg; Rogister, Bernard ULg et al

in Biochemical Pharmacology (1996), 52(9), 1399-405

We analysed how interactions between protein kinase-dependent intracellular signalling pathways were implicated in the control of the production of tissue-type plasminogen activator (tPA) and the ... [more ▼]

We analysed how interactions between protein kinase-dependent intracellular signalling pathways were implicated in the control of the production of tissue-type plasminogen activator (tPA) and the generation of neurite outgrowth by PC12 cells. To that aim, cells were treated with agents that interact with the trk receptor and with protein kinases A and C. Nerve growth factor induced only the formation of large neurites. The release of the protease and the production of short neurite outgrowth were found to be protein-kinase-A-dependent events that could be enhanced by simultaneous activation of protein kinase C with phorbol ester. At high concentration, staurosporine, a nonselective inhibitor of protein kinases, induced the production of short neurites and mimicked the protein-kinase-A-dependent effect on tPA release. Such a response was not observed with K-252a, an analogue of staurosporine devoid of neurite-outgrowth-promoting activity. The responses to protein kinase A stimulation and the addition of staurosporine, although similar, seemed to occur through an activation of distinct, yet interacting, signalling pathways. In conclusion, tPA release and large neurite outgrowth from PC12 cells are controlled by parallel, albeit interacting, pathways, suggesting that these two potentially antagonistic events in PC12 cell differentiation can be modulated in a concerted way or independently of each other, depending on the activity of several protein kinases. [less ▲]

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See detailSynthesis and properties of poly[isobornyl methacrylate (IBMA)-b-butadiene (BD)-b-IBMA] copolymers : new thermoplastic elastomers of a large service temperature range
Yu, Jian Ming; Dubois, Philippe ULg; Jérôme, Robert ULg

in Macromolecules (1996), 29(16), 7316-7332

Anionic polymerization of isobornyl methacrylate (IBMA) has been studied in THF and toluene in a temperature range from −78 to +40 °C by using (1,1-diphenyl-3,3-dimethylbutyl)lithium (DDBLi) as an ... [more ▼]

Anionic polymerization of isobornyl methacrylate (IBMA) has been studied in THF and toluene in a temperature range from −78 to +40 °C by using (1,1-diphenyl-3,3-dimethylbutyl)lithium (DDBLi) as an initiator in the presence of LiCl or not. Effect of solvent and polymerization temperature on tacticity has been studied. The reactivity of IBMA is comparable to tert-butyl methacrylate (tBMA), and polymers of a very narrow molecular weight distribution (<1.10) have been synthesized at room temperature, in THF, in the presence of LiCl. The Tg of PIBMA is found to vary from 170 to 206 °C with chain tacticity. Poly(isobornyl methacrylate) (PIBMA)−polybutadiene (PBD)−PIBMA triblock copolymers have been synthesized by using the m-diisopropenylbenzene (m-DIB)/tert-butyllithium (t-BuLi) diadduct as an initiator. The PBD midblock has been prepared in a cyclohexane/diethyl ether (100/6, v/v) mixture at room temperature. THF has been added [cyclohexane/diethyl ether/THF (100/6/100, v/v/v)] before the IBMA polymerization takes place at either −78 or +25 °C. Triblock copolymers of a very narrow molecular weight distribution (1.10) have been synthesized even at 25 °C, and no gel formation has been observed. These new triblock copolymers exhibit high tensile strength (30 MPa), high ultimate elongation (1000%), and high upper service temperature (160 °C). [less ▲]

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See detailNt-3 Has a Tropic Effect on Process Outgrowth by Postnatal Auditory Neurones in Vitro
Malgrange, Brigitte ULg; Lefebvre, P. P.; Martin, Didier ULg et al

in Neuroreport (1996), 7(15-17), 2495-9

CONFOCAL analysis of early postnatal auditory neurones in a bicompartmental culture system was used to test for chemoattractant properties of NGF, BDNF and NT-3 on neuronal process outgrowth. NT-3 exerted ... [more ▼]

CONFOCAL analysis of early postnatal auditory neurones in a bicompartmental culture system was used to test for chemoattractant properties of NGF, BDNF and NT-3 on neuronal process outgrowth. NT-3 exerted a strong tropic effect on neuritic outgrowth from auditory neurones in this system. BDNF and NGF did not have any tropic activity that directed processes outgrowth from auditory neurones. However, BDNF was important for the support of neuronal survival in NGF-treated cultures and for neuritogenesis in NT-3-treated cultures. Since NT-3 has been identified as both a survival factor and a chemotropic agent for auditory neurones, it is likely that this neurotrophin will be a useful therapeutic agent in the treatment of damaged cochleae for the recovery of hearing. [less ▲]

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See detailA method to optimize intermediate-passband photometric systems.
Royer, P.; Manfroid, Jean ULg

in Astronomy and Astrophysics. Supplement Series (1996), 119

A method is proposed to optimize the definition of existing photometric filters in order to minimize passband-matching problems. It is argued that the "improved" systems should generally yield better ... [more ▼]

A method is proposed to optimize the definition of existing photometric filters in order to minimize passband-matching problems. It is argued that the "improved" systems should generally yield better astrophysical parameters than the original ones. Although of a general scope, the method is checked on intermediate-passband filters of the Stroemgren system. [less ▲]

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See detailInfrared aperture photometry at ESO (1983-1994) and its future use.
van der Bliek, N. S.; Manfroid, Jean ULg; Bouchet, P.

in Astronomy and Astrophysics. Supplement Series (1996), 119

We describe the infrared (IR) photometric system for the single channel photometers at ESO, which have been used from 1983 until 1994. In addition to the broadband near infrared (NIR, 1-5μm) photometric ... [more ▼]

We describe the infrared (IR) photometric system for the single channel photometers at ESO, which have been used from 1983 until 1994. In addition to the broadband near infrared (NIR, 1-5μm) photometric system presented in 1991 by Bouchet et al. and Bersanelli et al., we describe a narrow-band NIR photometric system and a mid infrared (MIR, 7-20μm) photometric system. We also extend the set of NIR standard stars by Bouchet et al. towards fainter objects (K=~9). The photometric data of the standard stars in these systems were extracted from the complete IR photometric data archive of ESO, covering 10 years. The zeropoints of the NIR photometry are set by assuming that HR 3314 has a V-magnitude of 3.89, and that V-K=-0.05, J-K=-0.01, H-K=-0.01, K-L'=0.00, K-M=0.00. The zeropoints of the MIR photometry are set by assuming that the colours ofβ Hyi (HR 0098) and α CenA (HR 5459) are equal to the colours of the Sun. We adopt the absolute calibration of Megessier (1995A&A...296..771M) for the NIR and we argue that this calibration can be extrapolated to 20μm, using the MIR calibrations by Rieke et al. (1985AJ.....90..900R) and Cohen et al. (1992AJ....104.1650C). The definition of the zeropoints is consistent with the absolute calibration. We obtained accurate (Ï =~0.02mag.) NIR photometry of about 240 standard stars and MIR photometry of about 40 standard stars (Ï =~0.04mag). Comparison of our NIR photometric system with other well established systems shows that there are some small colour dependencies and zeropoint offsets which are always smaller than about 0.02mag. except for the L' band. [less ▲]

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See detailExpression of the 67-Kd Laminin Receptor, Galectin-1, and Galectin-3 in Advanced Human Uterine Adenocarcinoma
van den Brule, F. A.; Buicu, C.; Berchuck, A. et al

in Human Pathology (1996), 27(11), 1185-91

Alterations of tumor cell interactions with laminin, a basement membrane glycoprotein, are consistent features of the invasive and metastatic phenotype. Qualitative and quantitative changes in the ... [more ▼]

Alterations of tumor cell interactions with laminin, a basement membrane glycoprotein, are consistent features of the invasive and metastatic phenotype. Qualitative and quantitative changes in the expression of cell surface laminin-binding proteins have been correlated with the ability of cancer cells to cross basement membranes during the metastatic cascade. Such phenotypic modifications are usually associated with poor prognosis. In this study, the authors examined the possibility that expression of three laminin-binding proteins, the 67-kD laminin receptor (67LR), galectin-1, and galectin-3, is altered in human endometrial cancer in a fashion similar to that reported in other carcinomas, such as breast, colon, and ovarian cancer. Twenty advanced uterine adenocarcinomas were analyzed for expression of these three molecules using immunoperoxidase staining and specific antibodies. The authors found a significant increase in the expression of the 67LR and galectin-1 in cancer cells compared with normal adjacent endometrium (P = .0004 and .0022, respectively). As observed in other carcinomas, a significant down-regulation of galectin-3 expression was found in endometrial cancer cells compared with normal mucosa (P = .02). In the galectin-3 positive tumors, galectin-3 was detected in the cytoplasm and/or nucleus of cancer cells. Interestingly, tumors in which galectin-3 was detected only in the cytoplasm were characterized by deeper invasion of the myometrium than lesions where galectin-3 was found both in nucleus and cytoplasm (P = .02). This study shows an alteration of nonintegrin laminin-binding protein expression in advanced human endometrial cancer. Further studies on larger populations should determine the prognostic value of the detection of these laminin-binding proteins in endometrial carcinoma. Inverse modulation of the 67LR and galectin-3 appears to be a phenotypical feature of invasive carcinoma. [less ▲]

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See detail‘Bourgeoisie’, ‘massa’ en ‘volk’. Een kader voor de (re)constructie van Van Ostaijens ontwikkeling
Spinoy, Erik ULg

in Ons Erfdeel (1996), 39(5), 663-672

This article attempts to reframe the literary work of the modernist writer Paul van Ostaijen by focusing on his construction of his supposedly 'avant-gardist' position in society: anti-establishment and ... [more ▼]

This article attempts to reframe the literary work of the modernist writer Paul van Ostaijen by focusing on his construction of his supposedly 'avant-gardist' position in society: anti-establishment and anti-elitist, but also aloof of the 'ignorant' and gullible masses. [less ▲]

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See detailStructural and Functional Analysis of Bovine Herpesvirus 1 Minor Glycoproteins
Baranowski, Eric; Keil, Günther; Lyaku, Japhet et al

in Veterinary Microbiology (1996), 53(1-2), 91-101

This paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gH, gE, gG and gp42. It reviews the progress which has been made in their identification and characterization ... [more ▼]

This paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gH, gE, gG and gp42. It reviews the progress which has been made in their identification and characterization, in the study of their temporal expression and processing in infected cells, and finally in the understanding of their biological activities. In addition, aspects discussed include a comparison with two other alphaherpesviruses, namely herpes simplex virus and pseudorabies virus. [less ▲]

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See detailLes prions, aspects moleculaires
Rentier, Bernard ULg

in Revue Médicale de Liège (1996), 51(11), 700-705

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See detailPharma-clinics. Comment je traite... un patient diabétique de type 1 par pompe portable à insuline
Philips, J. C.; Scheen, André ULg

in Revue Médicale de Liège (1996), 51(11), 678-80

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See detailPolyimide foams derived from a high Tg polyimide with grafted poly(α-methylstyrene)
Hedrick, James L; DiPietro, R.; Plummer, C. J. G. et al

in Polymer (1996), 37(23), 5229-5236

A new route to high-Tg, thermally stable polyimide foams has been developed. Foams were prepared by casting microphase-separated graft copolymers comprising a thermally stable main chain, polyimide, and a ... [more ▼]

A new route to high-Tg, thermally stable polyimide foams has been developed. Foams were prepared by casting microphase-separated graft copolymers comprising a thermally stable main chain, polyimide, and a thermally labile graft, poly(α-methylstyrene). The copolymer compositions were designed so that the thermally labile block would be the dispersed phase. This can unzip to its monomer upon heating, and the decomposition product diffuses out of the film, leaving pores embedded in a matrix of the thermally stable component. The copolymers were synthesized through either the poly(amic acid) precursor, followed by chemical cyclodehydration to the imide form, or the poly(amic alkyl ester) precursor followed by thermal imidization. The decomposition of the α-methylstyrene in the block copolymer was studied by thermogravimetric, dynamic mechanical and thermomechanical analyses. Mild decomposition conditions were required to avoid rapid depolymerization of the α-methylstyrene and excessive plasticization of the polyimide matrix. The foams showed pore sizes with diameters ranging from less than 20 nm to over 1 µm, depending upon the synthetic route employed, and the reduction in the mass density was generally consistent with the starting composition. [less ▲]

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See detailInfluence de l'association du nomegestrol acetate sur l'amélioration de la qualité de vie induite par une estrogenotherapie chez la femme ménopausée
Reginster, Jean-Yves ULg; Zartarian, M.; Colau, J. C.

in Contraception, Fertilite, Sexualité (1992) (1996), 24(11), 847-51

Estrogen replacement therapy in postmenopausal women is followed by several benefits including a prompt improvement of quality of life. Due to uterine proliferation induced by prolonged estrogen intake ... [more ▼]

Estrogen replacement therapy in postmenopausal women is followed by several benefits including a prompt improvement of quality of life. Due to uterine proliferation induced by prolonged estrogen intake, progestogens are usually associated, on a cyclical basis, in hormonal replacement therapy prescribed to non-hysterectomized women. Persistence of the beneficial effect of estrogen on quality of life, during progestogens intake, was never investigated. We evaluated, through a specific and previously validated questionnaire, the changes in quality of life observed in 351 women recently menopaused, after 6 months of hormonal replacement therapy associating 24 days of estrogens and 12 days of administration of a non androgenic progestogen: nomegestrol acetate. Patients were randomized within two therapeutic groups in which evaluation of quality of life was performed either during the administration of estrogen alone or during administration of estrogen and progestogen. In the two groups, hormonal replacement therapy was followed by a significant improvement (P < 0,001) in quality of life and no significant difference was observed between the changes observed during estrogen or estrogen-progestogen administration. Quality of life indices measured after 6 months of hormonal replacement therapy are within the same range than values previously described in eugonadal women. We conclude that improvement of quality of life induced by estrogen replacement therapy remains unchanged during cyclical administration of nomegestrol acetate. [less ▲]

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See detailA toy model for life at the "edge of chaos"
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Computers & Graphics (1996), 20(6), 921-923

In this short paper, we illustrate the emergence of complex structures from a simple model of life at the "edge of chaos". The visualization of the generated patterns provides a good way for understanding ... [more ▼]

In this short paper, we illustrate the emergence of complex structures from a simple model of life at the "edge of chaos". The visualization of the generated patterns provides a good way for understanding the self-organized critical dynamics of the model. [less ▲]

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See detailSubset-Specific Analysis of Calcium Fluxes in Murine Aids
Moutschen, Michel ULg; Trebak, M.; Greimers, Roland ULg et al

in International Immunology (1996), 8(11), 1715-27

Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and ... [more ▼]

Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and lymphoproliferation. In addition to a complete refractoriness of most subsets of lymphocytes to mitogen stimulation, the development of phenotypic abnormalities occurs such as the appearance of an abnormal CD4+ T cell subset lacking membranes Thy-1. This study was performed to compare the calcium responses during the early stages of MAIDS (week 9 or earlier) between T cells and B cells and between CD4+Thy-1- and CD4+Thy-1+ T cells. B cells were strikingly less affected than T cells: their baseline [Ca2+]i did not significantly increase, and their calcium response to anti-IgM antibody and concanavalin A (Con A) was partially maintained. In contrast, the response to Con A was completely abolished in T cells. Interestingly, calcium mobilization in response to membrane receptor-independent stimuli such as ionophores and thapsigargin was strongly inhibited in T cells, while no such inhibition was found in B cells. In comparison with their CD4+Thy-1+ counterparts, CD4+Thy-1- T cells had blunted calcium responses in controls, as well as in infected mice. However, CD4+Thy-1+ T cells were also strikingly altered, suggesting that the loss of membrane Thy-1 could be associated with, but not directly responsible for abnormalities of calcium responses in CD4+ T cells from RadLV-Rs-infected mice. [less ▲]

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See detailLessons to be learned from varicella-zoster virus
Rentier, Bernard ULg; Piette, Jacques ULg; Baudoux, Laurence et al

in Veterinary Microbiology (1996), 53(1-2), 55-66

Varicella-zoster virus (VZV) is an alphaherpesvirus responsible for two human diseases: chicken pox and shingles. The virus has a respiratory port of entry. After two successive viremias, it reaches the ... [more ▼]

Varicella-zoster virus (VZV) is an alphaherpesvirus responsible for two human diseases: chicken pox and shingles. The virus has a respiratory port of entry. After two successive viremias, it reaches the skin where it causes typical lesions. There, it penetrates the peripheral nervous system and it remains latent in dorsal root ganglia. It is still debatable whether VZV persists in neurons or in satellite cells. During latency, VZV expresses a limited set of transcripts of its immediate early (IE) and early (E) genes but no protein has been detected. Mechanisms of reactivation from ganglia have not been identified. However, dysfunction of the cellular immune system appears to be involved in this process. The cell-associated nature of VZV has made it difficult to identify a temporal order of gene expression, but there appears to be a cascade mechanism as for HSV-1. The lack of high titre cell-free virions or recombination mutants has hindered so far the understanding of VZV gene functions. Five genes, ORFs 4, 10, 61, 62, and 63 that encode regulatory proteins could be involved in VZV latency. ORF4p activates gene promoters with basal activities. ORF10p seems to activate the ORF 62 promoter. ORF61p has trans-activating and trans-repressing activities. The major IE protein ORF62p, a virion component, has DNA-binding and regulatory functions, transactivates many VZV promoters and even regulates its own expression. ORF63p is a nuclear IE protein of yet unclear regulatory functions, abundantly expressed very early in infection. We have established an animal model of VZV latency in the rat nervous system, enabling us to study the expression of viral mRNA and protein expression during latency, and yielding results similar to those found in humans. This model is beginning to shed light on the molecular events in VZV persistent infection and on the regulatory mechanisms that maintain the virus in a latent stage in nerve cells. [less ▲]

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See detailJorge Luis Borges
Strivay, Lucienne ULg

in Voir (1996), 12-13(novembre), 43-50

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See detailSamuel Beckett
Strivay, Lucienne ULg

in Voir (1996), 12-13(novembre), 30-40

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See detailInfluence of Several Peptidase Inhibitors on the Pro-Inflammatory Effects of Substance P, Capsaicin and Collagenase
Damas, Jacques ULg; Bourdon, V.; Liégeois, Jean-François ULg et al

in Naunyn-Schmiedeberg's Archives of Pharmacology (1996), 354(5), 662-9

Injection of substance P (SP) in a rat hindpaw induced extravasation of 125I-labelled albumin in both hindpaws and salivation. Intravenous injection of SP dose-dependently increased vascular permeability ... [more ▼]

Injection of substance P (SP) in a rat hindpaw induced extravasation of 125I-labelled albumin in both hindpaws and salivation. Intravenous injection of SP dose-dependently increased vascular permeability. This latter effect was increased in rat paws by captopril, an inhibitor of angiotensin-converting enzyme (ACE), administered locally in combination with diprotin A, an inhibitor of an dipeptidyl(amino)peptidase IV (DAP IV) or phosphoramidon, an inhibitor of neutral endopeptidase (NEP). The increase in permeability induced by SP was inhibited by RP 67580, a NK-1-receptor antagonist. Intravenous injection of capsaicin induced labelled albumin extravasation in rat paws. This effect was increased by combination of captopril with diprotin A or phosphoramidon, but not by captopril associated with amastatin, an inhibitor of aminopeptidase M (AmM). It was suppressed by RP 67580. Injection of collagenase in rat paws triggered a swelling and a local plasma exudation. These responses were reduced by RP 67580 but not by RP 68651, its inactive enantiomer. They were increased by combination of captopril with diprotin A or phosphoramidon in normal rats. The potentiating effects of captopril and diprotin A were suppressed by RP 67580 in normal rats but did not develop in kininogen-deficient rats. The oedema induced by collagenase was also increased by lisinopril, another ACE inhibitor, administered locally in combination with apstatin, an inhibitor of aminopeptidase P (AmP). In rats pretreated by methysergide, collagenase-induced oedema was reduced and can be increased by captopril, by lisinopril, administered alone or by lisinopril associated with apstatin. It is concluded that SP is mainly inactivated in rat paws by ACE, DAP IV and NEP. In collagenase-induced oedema, a low amount of SP would be released from afferent nerve terminals by bradykinin formed in low amounts. Bradykinin is inactivated in rat paws by ACE and AmP. In collagenase-oedema, the pro-inflammatory effects of bradykinin are concealed by the effects of the other mediators. [less ▲]

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See detailCardiac Performance in Conscious Healthy Dogs During Dobutamine Infusion
Mc Entee, Kathleen ULg; Clercx, Cécile ULg; Pypendop, B. et al

in Research in Veterinary Science (1996), 61(3), 234-9

The aim of this study was to determine the cardiac performance of conscious healthy dogs during stimulation with dobutamine. Eight healthy unsedated beagle dogs were used. Cardiac output was measured by ... [more ▼]

The aim of this study was to determine the cardiac performance of conscious healthy dogs during stimulation with dobutamine. Eight healthy unsedated beagle dogs were used. Cardiac output was measured by the thermodilution technique and blood pressures by extravascular pressure transducers. Dobutamine challenge at a dosage ranging from 27.5 to 50 micrograms kg-1 min-1 induced a significant rise in cardiac power index (CPI), cardiac index (CI), stroke index (SI) and heart rate (HR) and a significant decrease in pulmonary vascular resistance (PVR) and systemic vascular resistance (SVR). The highest CPI was 2.05 times greater than its basal resting value. The CI was primarily responsible for this increase in CPI. The SI and HR contributed approximately 55 per cent and 45 per cent respectively of the maximal increase in CI. [less ▲]

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See detailThe Role of Glycoproteins gC, gE, gI and gG in the Induction of Cell-Mediated Immune Responses to Bovine Herpesvirus 1
Denis, M.; Hanon, E.; Rijsewijk, F. A. et al

in Veterinary Microbiology (1996), 53(1-2), 121-32

Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of various ... [more ▼]

Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of various gene deletions (gC, gE, gI, gG) on the induction of cell-mediated immune responses against the virus. The highest BHV1 specific lymphoproliferative response was observed in the group of calves inoculated with the gC- mutant. However, in all groups of inoculated calves, limiting dilution analysis showed marked individual variability in the number of BHV1 specific T lymphocytes that were stimulated. The same animals were then challenged with wild-type BHV1. In these animals, limiting dilution analysis did not reveal gE, gI nor gG as a major T lymphocyte antigen. However, further analysis suggested the T cell antigenicity of gE in a low number of BHV1 hyperimmunized calves. Stimulation of MHC unrestricted cytotoxicity was also evaluated after inoculation with the various deletion mutants. Cytotoxicity in gC- inoculated calves was as high as in BHV1 inoculated calves. In conclusion, among the BHV1 deletion mutants that were tested, the gC- mutant stimulated the best cell-mediated immune responses. [less ▲]

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See detail« Les Aveugles », lecture du sonnet de Charles Baudelaire
Durand, Pascal ULg

in Voir. Périodique du centre d'étude des aspects culturels de la vision (1996), 12/13

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See detailElectrochemical synthesis and characterization of the electronic structure of new conjugated polymers from dithienylene vinylene derivatives
Jérôme, Christine ULg; Maertens, Christophe; Mertens, Marc et al

in Synthetic Metals (1996), 83(2), 103-109

Highly conjugated thiophene derivatives, based on thienylene vinylene and thienylene vinylene phenylene units, have been synthesized in order to examine: (i) the effect of such regular conjugated monomer ... [more ▼]

Highly conjugated thiophene derivatives, based on thienylene vinylene and thienylene vinylene phenylene units, have been synthesized in order to examine: (i) the effect of such regular conjugated monomer structures on polymer formation; and (ii) the optical and electrochemical properties of the corresponding macromolecules. The all-trans monomers have been prepared by a Wittig reaction. The polymers have then been synthesized electrochemically. The polymers are electrochromic and can be reversibly doped both oxidatively and reductively. Their electrochemical behavior and optical properties have been analyzed on the basis of a series of quantum-chemical calculations. [less ▲]

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See detailBovine Herpesvirus 4: Genomic Organization and Relationship with Two Other Gammaherpesviruses, Epstein-Barr Virus and Herpesvirus Saimiri
Lomonte, P.; Bublot, M.; van Santen, V. et al

in Veterinary Microbiology (1996), 53(1-2), 79-89

Bovine herpesvirus 4 (BHV-4) belongs to the gammaherpesvirinae subfamily. Although the whole sequence of BHV-4 genome is not known it was possible, based on random sequencing, to assume that its genomic ... [more ▼]

Bovine herpesvirus 4 (BHV-4) belongs to the gammaherpesvirinae subfamily. Although the whole sequence of BHV-4 genome is not known it was possible, based on random sequencing, to assume that its genomic organization consists of genes clustered in blocks whose orientation and location in the genome are conserved within a herpesvirus subfamily. Between these blocks lie genes which are specific to either a particular virus or a virus subfamily. BHV-4 genome consists of 5 gene blocks conserved among the gammaherpesviruses and particularly within the Epstein-Barr virus (EBV) and the herpesvirus saimiri (HVS) genomes. Analysis of the regions located outside the gene blocks showed the presence of 12 open reading frames (ORFs). Protein database comparisons showed that no ORF translation products were similar to proteins encoded by alpha- or beta-herpesviruses. Nevertheless, 5 ORFs were homologous in amino acid sequences to proteins encoded by HVS and one was similar to a protein encoded by both HVS and EBV. On the basis of the molecular data BHV-4 is more closely related to HVS than to EBV. Genes homologous to cellular genes have been described in both HVS and EBV genomes. No genes homologous to presently sequenced cellular genes were found among those found in the BHV-4 genome to date. [less ▲]

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See detailL'image du mois. Epulis et diapneusie
Pierard, Gérald ULg

in Revue Médicale de Liège (1996), 51(11), 677

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See detailTraitement du cancer broncho-pulmonaire par carboplatine/etoposide : étude de survie
Skaventzos, I.; Weber, T.; Bury, Thierry ULg et al

in Revue Médicale de Liège (1996), 51(11), 718-20

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See detailPotentiation of the Pro-Inflammatory Effects of Bradykinin by Inhibition of Angiotensin-Converting Enzyme and Aminopeptidase P in Rat Paws
Damas, Jacques ULg; Liégeois, Jean-François ULg; Simmons, W. H.

in Naunyn-Schmiedeberg's Archives of Pharmacology (1996), 354(5), 670-6

The influence of some peptidase inhibitors on oedema and plasma extravasation induced by bradykinin and carrageenan in rat paw was evaluate. Bradykinin-induced oedema in normal rats was increased by o ... [more ▼]

The influence of some peptidase inhibitors on oedema and plasma extravasation induced by bradykinin and carrageenan in rat paw was evaluate. Bradykinin-induced oedema in normal rats was increased by o-phenanthroline (3.10(-2) M), by captopril (10(-6) M to 10(-4) M), by lisinopril (10(-6) M to 10(-4), or by lisinopril (10(-5) M) in combination with apstatin (8.10(-5) M or 1.4 10(-4) M). It was not modified by phosphoramidon (10(-6) M to 10(-5) M) and by diprotin A (10(-3) M). It was increased by mergepta at high concentrations (2.10(-4) M). Mergepta did not increase the potentiating effect of captopril. Carrageenan-oedema in normal rats was increased by captopril (10(-5) M), lisinopril (10(-5) M) and apstatin (1.4 10(-4) M. It was not modified by mergepta (10(-4) M), phosphoramidon (10 (-5) M) and diprotin A (109-3) M). Des-Arg1-bradykinin and Des-Arg9-bradykinin have low oedema-promoting effects. Captopril (10(-5) M) increased the effects of bradykinin but not those of carrageenan in kininogen-deficit Brown Norway rats. Angiotensin-converting enzyme and aminopeptidase P appear to be main kinin-inactivating enzymes in rat paws. Carboxypeptidase N, neutral endopeptidase 24.11 and dipeptidyl(amino)peptidase IV do not play a significant role in this inactivation. [less ▲]

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See detailUmberto Eco
Strivay, Lucienne ULg

in Voir (1996), 12-13(novembre), 87-91

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See detailLe diabète insulinodépendant
Geenen, Vincent ULg

in Revue Médicale de Liège (1996), 51(11), 684-94

Par son caractère chronique et la gravité de ses complications à long terme, le diabète insulino-dépendant (DID) constitue une véritable leçon de modestie pour le médecin à la fin de ce siècle. Le DID est ... [more ▼]

Par son caractère chronique et la gravité de ses complications à long terme, le diabète insulino-dépendant (DID) constitue une véritable leçon de modestie pour le médecin à la fin de ce siècle. Le DID est un modèle de maladie autoimmune spécifique d'organe et même d'une cellule particulière, la cellule ß insulino-sécrétrice des îlots de Langerhans dans le pancréas. Parler de maladie autoimmune signifie aussi rupture de la tolérance immunitaire vis-à-vis d'un composant du Soi. La connaissance des mécanismes responsables de la tolérance de la cellule ß et la compréhension des phénomènes génétiques et environnementaux impliqués dans sa rupture permettront la mise au point d'une prévention efficace, voire d'une éradication complète du DID. La vaccination 'tolérogène' basée sur la connaissance des antigènes thymiques responsables de l'éducation immunitaire au Soi constitute une de ces voies d'avenir prometteuse. [less ▲]

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See detailFractal grain boundaries in growth competition
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg; Cloots, Rudi ULg

in Journal of Crystal Growth (1996), 169(1), 79-82

Evidence of fractal grain boundaries is found in polycrystals when radial patterns are found to result from a geometrical competition between different grains that have nucleated on the same center. A ... [more ▼]

Evidence of fractal grain boundaries is found in polycrystals when radial patterns are found to result from a geometrical competition between different grains that have nucleated on the same center. A simple statistical model presented herein predicts the universal fractal dimension 2.33 of such natural patterns grown through internal competition. The application to material patterns having constrained front propagation involving many components is obvious. [less ▲]

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See detailIntermolecular interactions in poly(vinylidene fluoride) and ε-caprolactam mixtures
Liu, Zehui; Maréchal, Philippe; Jérôme, Robert ULg

in Polymer (1996), 37(23), 5317-5320

Mixtures of poly(vinylidene fluoride) (PVDF) and caprolactam (CPL) have been investigated as models for possible cross-interactions between vinylidene fluoride and amide structural units, that might ... [more ▼]

Mixtures of poly(vinylidene fluoride) (PVDF) and caprolactam (CPL) have been investigated as models for possible cross-interactions between vinylidene fluoride and amide structural units, that might account for the very fine phase morphology previously observed in PVDF/polyamide 6 blends. Over the entire composition range, the PVDF/CPL mixtures are monophase above the PVDF melting temperature. From the depression of the PVDF melting point, a negative interaction energy density, B, has been calculated that, however, depends on both temperature and CPL concentration. Mutual solubility of PVDF and CPL and negative B values are consistent with specific intermolecular interactions whose nature is discussed. [less ▲]

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See detailAutomated Determination of Verapamil and Norverapamil in Human Plasma with on-Line Coupling of Dialysis to High-Performance Liquid Chromatography and Fluorometric Detection
Ceccato, Attilio ULg; Chiap, Patrice ULg; Hubert, Philippe ULg et al

in Journal of Chromatography. A (1996), 750(1-2), 351-60

A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using ... [more ▼]

A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using dialysis followed by clean-up and enrichment of the dialysate on a precolumn and subsequent HPLC analysis with fluorometric detection. All sample handling operations were performed automatically by a sample processor equipped with a robotic arm (ASTED system). The plasma samples were dialysed on a cellulose acetate membrane (cut-off: 15 kD) and the dialysate was purified and enriched on a short pre-column filled with cyanopropyl silica. Before starting dialysis, this trace enrichment column (TEC) was first conditioned with the HPLC mobile phase and then with pH 3.0 acetate buffer. 370 microliters of plasma sample spiked with the internal standard (gallopamil) were dialysed in the static-pulsed mode. The solution at the donor side was pH 3.0 acetate buffer containing Triton X-100 while the acceptor solution was made of the same acetate buffer. When dialysis was discontinued, the analytes were desorbed from the TEC by the HPLC mobile phase and transferred to the C18 analytical column by means of a switching valve. This mobile phase consisted of a mixture of acetonitrile, pH 3.0 acetate buffer and 2-aminoheptane. The influence of different parameters of the dialysis process on the recovery of verapamil and norverapamil has been studied. The effect of the volume, the aspirating and dispensing flow-rates of the dialysis solution has been investigated. The recoveries of verapamil and norverapamil in plasma were close to 75% and the limits of quantification were 5 ng/ml for both analytes. The method was found to be linear in the concentration range from 5 to 500 ng/ml (r2: 0.9996 for both analytes). The intra-day and inter-day reproducibilities at a concentration of 100 ng/ml were 2.3% and 5.6% for verapamil and 1.7% and 5.1% for norverapamil, respectively. [less ▲]

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See detailPrincipe de proportionnalité et droit des sociétés
Partsch, Philippe-Emmanuel ULg

in Journal des Tribunaux (1996)

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See detailTranscription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: implications for nucleotide excision repair and Cockayne syndrome
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Proceedings of the National Academy of Sciences of the United States of America (1996), 93(20), 10718-22

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See detailPrévention de l'ostéoporose postménopausique: œstrogènes et thérapeutiques non hormonales
Reginster, Jean-Yves ULg

in Revue Médicale de Liège (1996), 51(10), 649-53

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See detailL'accompagnement d'équipes éducatives dans la mise en place de la réforme du premier degré: parcours scolaires et pratiques de différenciation
Guillaume, Marie-Elisabeth; Hindryckx, Marie-Noëlle ULg

in Informations pédagogiques (1996), 29

Synthèse d'une année de recherche-action basée sur l'accompagnement d'équipes éducatives dans la mise en place de la réforme du premier degré du secondaire : chronique d'une collaboration. Le travail ... [more ▼]

Synthèse d'une année de recherche-action basée sur l'accompagnement d'équipes éducatives dans la mise en place de la réforme du premier degré du secondaire : chronique d'une collaboration. Le travail collaboratif s'est effectué avec des équipes de professeurs de français de l'Athénée de Visé. Les axes de travail choisis furent les suivants :définir les compétences minimales qui devront être maîtrisées par les élèves au terme du premier degré de l'enseignement secondaire - concevoir et mettre au point de nouvelles pratiques pédagogiques et de nouveaux outils visant la construction des compétences choisies pour la première année du cycle - Intégrer des démarches d'évaluation diagnostique (quels savoirs, quelles compétences sont acquises), d'évaluation formative (repérage et analyse des difficultés d'apprentissage rencontrées par les élèves) et de pratiques de différenciation - Expérimenter les actions éducatives conçues - Evaluer celles-ci, analyser les paramètres qui ont présider à leur élaboration. [less ▲]

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See detailStatic and dynamic epidemics on looped chains and looped trees
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1996), 54(4), 3499-3507

The dynamic epidemic model [N. Vandewalle and M. Ausloos, J. Phys. A 29, 309 (1996)] considers the growth of a cluster in a medium containing a fraction x of mobile "particles" that are pushed by a ... [more ▼]

The dynamic epidemic model [N. Vandewalle and M. Ausloos, J. Phys. A 29, 309 (1996)] considers the growth of a cluster in a medium containing a fraction x of mobile "particles" that are pushed by a propagation front. This model is exactly solved here on various chains and trees that contain loops following an "evolution matrix" method. The exact value for the percolation threshold x(c) and the critical exponents are calculated for static and mobile particles, respectively. Surprisingly, the mobile character of the particles affects the values of the critical exponents on chains but not on trees. Thus there is a nonuniversal behavior for dynamic epidemics even on d=1 lattices. [less ▲]

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See detailRegional brain activity during working memory tasks
Salmon, Eric ULg; Van der Linden, Martial ULg; Collette, Fabienne ULg et al

in Brain (1996), 119(Pt 5), 1617-1625

The first aim of our PET study was to replicate previous findings concerning the brain areas activated by a verbal working memory task. The second aim was to specify the neural basis of the central ... [more ▼]

The first aim of our PET study was to replicate previous findings concerning the brain areas activated by a verbal working memory task. The second aim was to specify the neural basis of the central executive, using a task of working memory updating. Our data confirm that the lower left supramarginal gyms and premotor area are the key regions subserving short-term verbal memory processes. They also suggest that the updating memory task is related to mid-dorsolateral prefrontal activation most probably responsible for the updating function of the central executive. An unexpected, predominantly right activation occurred in the inferior parietal region during the verbal memory updating task, which we related to a visuospatial strategy used to maintain the information in short-term memory. A third purpose was to explore the brain regions activated by a nonverbal, visual memory task, and our results confirm the importance of the superior occipital gyrus in the visual short-term memory. [less ▲]

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See detailMolecular Basis of Neuronal Biorhythms and Paroxysms
Grisar, Thierry ULg; Lakaye, Bernard ULg; Thomas, Elizabeth

in Archives of Physiology & Biochemistry (1996), 104(6), 770-4

The molecular basis of the biorhythms are evoked in relation to cerebral EEG rhythms and paroxysms. Basic oscillatory phenomena have been well shown and modeled in systems such as the glycolytic pathway ... [more ▼]

The molecular basis of the biorhythms are evoked in relation to cerebral EEG rhythms and paroxysms. Basic oscillatory phenomena have been well shown and modeled in systems such as the glycolytic pathway, the oscillations of cAMP in amoebas and rhythms of the intracellular cycline during mitosis. In excitable cells the intracellular calcium and cAMP oscillations exhibit a signalling system with many advantages. Thus the question arises: to what extent can the EEG paroxysms observed in epileptic syndrome be due to disturbances in such basic molecular pathways that underlie intracellular molecular oscillations? The usefulness of the absence-rat-model and the implication the T type Ca(2+)-channel of the thalamic nuclei in the pathophysiology of this epileptic syndrome are discussed. [less ▲]

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See detailVimentin Expression in Cervical Carcinomas: Association with Invasive and Migratory Potential
Gilles, Christine ULg; Polette, M.; Piette, Jacques ULg et al

in Journal of Pathology (The) (1996), 180(2), 175-80

Vimentin is an intermediate filament protein normally expressed in mesenchymal cells, but evidence is accumulating in the literature which suggests that the aberrant expression of vimentin in epithelial ... [more ▼]

Vimentin is an intermediate filament protein normally expressed in mesenchymal cells, but evidence is accumulating in the literature which suggests that the aberrant expression of vimentin in epithelial cancer cells might be related to local invasiveness and metastatic potential. Vimentin expression has previously been associated with invasive properties in an in vitro model consisting of a set of HPV-33-transformed cervical keratinocyte cell lines. In the present study, in order to emphasize those in vitro findings, the expression of vimentin has been investigated in cervical neoplasms of different grades, using immunohistochemistry. A clear association is reported between vimentin expression and metastatic progression, since vimentin was detected in all invasive carcinomas and lymph node metastases, but not in CIN III lesions. These in vivo results are compared with present and previous data obtained in vitro on cervical keratinocyte cell lines, where vimentin expression also correlated with in vitro invasiveness. [less ▲]

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See detailThe numerical simulation and physical model test of pipeline under water action
Marchal, Jean ULg; Yucheng, Li; Guozhang, Lai et al

in Proceedings of the Fourth Pacific/Asia Offshore Mechanics Symposium, Pusan, Korea (1996)

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See detailIdentification of Post-Transplant Anti-Alpha 5 (Iv) Collagen Alloantibodies in X-Linked Alport Syndrome
Dehan, Pierre ULg; Van den Heuvel, L. P.; Smeets, H. J. et al

in Nephrology Dialysis Transplantation (1996), 11(10), 1983-8

X-linked Alport syndrome (AS) is a heritable disorder which is associated with mutations in the type IV collagen alpha 5 (IV) chain gene (COL4A5) located on chromosome X. Following renal transplantation ... [more ▼]

X-linked Alport syndrome (AS) is a heritable disorder which is associated with mutations in the type IV collagen alpha 5 (IV) chain gene (COL4A5) located on chromosome X. Following renal transplantation, an average of 6% of male AS patients develop anti-GBM nephritis. We studied the specificity of the antibodies against type IV collagen in the serum of a patient with COL4A5 partial deletion. The specificity of these alloantibodies was determined against collagenase-digested GBM, as well as against recombinant non-collagenous (NC1) domains of the type IV collagen alpha 1(IV)-alpha 6(IV) chains expressed in escherichia coli. Immunoblotting and ELISA demonstrated that these antibodies bound specifically to the NC1 domain of alpha 5(IV) collagen. There was no binding to the NC1 domain of the other chains, including the Goodpasture antigen. Competitive ELISA confirmed the results obtained by ELISA and immunoblotting. This patient developed alloantibodies directed against antigens present in the grafted kidney, but absent from his Alport kidney. The pathogenesis of post-transplantation glomerulonephritis in the Alport patient studied is thus similar to that of Goodpasture syndrome, with the exception that the pathogenic antibodies are targeted to another alpha chain of type IV collagen. [less ▲]

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See detailDistribution of Aromatase-Immunoreactive Cells in the Forebrain of Zebra Finches (Taeniopygia Guttata): Implications for the Neural Action of Steroids and Nuclear Definition in the Avian Hypothalamus
Balthazart, Jacques ULg; Absil, Philippe ULg; Foidart, Agnès ULg et al

in Journal of Neurobiology (1996), 31(2), 129-48

Cells immunoreactive for the enzyme aromatase were localized in the forebrain of male zebra finches with the use of an immunocytochemistry procedure. Two polyclonal antibodies, one directed against human ... [more ▼]

Cells immunoreactive for the enzyme aromatase were localized in the forebrain of male zebra finches with the use of an immunocytochemistry procedure. Two polyclonal antibodies, one directed against human placental aromatase and the other directed against quail recombinant aromatase, revealed a heterogeneous distribution of the enzyme in the telencephalon, diencephalon, and mesencephalon. Staining was enhanced in some birds by the administration of the nonsteroidal aromatase inhibitor, R76713 racemic Vorozole) prior to the perfusion of the birds as previously described in Japanese quail. Large numbers of cells immunoreactive for aromatase were found in nuclei in the preoptic region and in the tuberal hypothalamus. A nucleus was identified in the preoptic region based on the high density of aromatase immunoreactive cells within its boundaries that appears to be homologous to the preoptic medial nucleus (POM) described previously in Japanese quail. In several birds alternate sections were stained for immunoreactive vasotocin, a marker of the paraventricular nucleus (PVN). This information facilitated the clear separation of the POM in zebra finches from nuclei that are adjacent to the POM in the preoptic area-hypothalamus, such as the PVN and the ventromedial nucleus of the hypothalamus. Positively staining cells were also detected widely throughout the telencephalon. Cells were discerned in the medial parts of the ventral hyperstriatum and neostriatum near the lateral ventricle and in dorsal and medial parts of the hippocampus. They were most abundant in the caudal neostriatum where they clustered in the dorsomedial neostriatum, and as a band of cells coursing along the dorsal edge of the lamina archistriatalis dorsalis. They were also present in high numbers in the ventrolateral aspect of the neostriatum and in the nucleus taeniae. None of the telencephalic vocal control nuclei had appreciable numbers of cells immunoreactive for aromatase within their boundaries, with the possible exception of a group of cells that may correspond to the medial part of the magnocellular nucleus of the neostriatum. The distribution of immunoreactive aromatase cells in the zebra finch brain is in excellent agreement with the distribution of cells expressing the mRNA for aromatase recently described in the finch telencephalon. This widespread telencephalic distribution of cells immunoreactive for aromatase has not been described in non-songbird species such as the Japanese quail, the ring dove, and the domestic fowl. [less ▲]

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See detailL'écologie, la photoclimatologie et la géographie vont-elles influencer la dermatologie de demain?
Pierard, Gérald ULg

in Revue Médicale de Liège (1996), 51(10), 647-8

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See detailSAXS analysis of the morphology of biocompatible and biodegradable poly(ε-caprolactone-b-glycolide) copolymers1
Sobry, R.; Van den Bossche, Guy ULg; Fontaine, F. et al

in Journal of Molecular Structure (1996), 383(1-3), 63-68

Poly(epsilon-caprolacrone-b-glycolide) diblock copolyesters have been synthesized by the sequential polymerization of epsilon-caprolactone and glycolide as initiated by aluminium alkoxides ... [more ▼]

Poly(epsilon-caprolacrone-b-glycolide) diblock copolyesters have been synthesized by the sequential polymerization of epsilon-caprolactone and glycolide as initiated by aluminium alkoxides. Copolymerization is typically ''living'' and yields copolyesters of perfectly controlled molecular weight and composition. Diblock molecular weight (Mn-PGA+Mn-PCL) ranges from 5700 to 42000 and the rho = Mn-PCL/Mn-PGA ratio varies from 1.5 to 13.1. Due to the inherent insolubility of the polyglycolide (PGA) segment in common organic solvent, the diblock copolyesters form stable non-aqueous colloidal dispersions e.g. in toluene, the stability of which results from the soluble poly(epsilon-caprolactone) (PCL) block. Combining all the experimental observations (PCS, TEM, WAXS, SAXS, AFM), a micelle model has been proposed which consists of a polyglycolide core surrounded with a corona of polycaprolactone (PCL). Both constituents are semi crystalline. From SAXS observations, the PGA core is better described by two concentric spheres. The internal sphere of a 5-6.7 nn diameter would essentially contain crystalline PGA. The diameter of the external sphere, D-PGA, is in the range from 6.2 to 9.6 nm, at least for the investigated diblock copolymers. As a rule, this diameter increases as rho decreases at constant molecular weight and as the diblock molecular weight increases at constant rho. A scattering peak (weak) is observed in the range from 10.8 to 15.5 nm and the Bragg distance is close mu D-PGA, where mu is equal to (1+3 rho/2)(1/3). From steric considerations, mu is the ratio between the diameter of the micelle and the diameter of the PGA core, so that this peak has been assigned to the characteristic intermicellar distance. At very small angles, several additional peaks are the signature of a hyperstructure which is possibly lamellar. [less ▲]

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See detailStructural and Functional Aspects of Chloride Binding to Alteromonas Haloplanctis Alpha-Amylase
Feller, Georges ULg; le Bussy, O.; Houssier, C. et al

in Journal of Biological Chemistry (1996), 271(39), 23836-41

Chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the bacterial alpha-amylase from Alteromonas haloplanctis. Activation experiments of A. haloplanctis alpha ... [more ▼]

Chloride is the allosteric effector of vertebrate pancreatic and salivary alpha-amylases and of the bacterial alpha-amylase from Alteromonas haloplanctis. Activation experiments of A. haloplanctis alpha-amylase by several monovalent anions show that a negative charge, not restricted to that of Cl-, is essential for the amylolytic reaction. Engineering of the chloride binding site reveals that a basic residue is an essential component of the site. The mutation K337R alters the Cl--binding properties, whereas the mutation K337Q produces an active, chloride-independent enzyme. Comparison of the Kd values for Cl- in three homologous alpha-amylases also indicates that the binding affinity is dependent on the chloride coordination mode by this basic residue. Analysis of substrate and chloride binding according to the allosteric kinetic model shows that the chloride effector is not involved in substrate binding. By contrast, the pH dependence of activity and experiments of chemical modifications and Ca2+ inhibition show that the chloride ion is responsible for the pKa shift of catalytic groups and interacts with active site carboxyl groups. [less ▲]

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See detailObservations on the Helminths of Harbour Porpoises (Phocoena Phocoena) and Common Guillemots (Uria Aalge) from the Belgian and German Coasts
Brosens, L.; Jauniaux, Thierry ULg; Siebert, U. et al

in Veterinary Record : Journal of the British Veterinary Association (1996), 139(11), 254-7

Between February 1990 and July 1991, 18 harbour porpoises (Phocoena phocoena) and 248 common guillemots (Uria aalge), found dead along the Belgian and German coasts, were examined for their burden of ... [more ▼]

Between February 1990 and July 1991, 18 harbour porpoises (Phocoena phocoena) and 248 common guillemots (Uria aalge), found dead along the Belgian and German coasts, were examined for their burden of helminths. A total of three species were found in the guillemots (one cestode, one nematode and one pentastomid), and six species in the porpoises (one trematode, one cestode and four nematodes). Among the guillemots the burden of helminths was not statistically different between juvenile and adult birds. The deaths of the birds were apparently not related to the parasite infections. In contrast, the adult porpoises were more heavily parasitised than the juveniles, except for one young porpoise stranded on the Belgian coast. In the porpoises, four species of parasites had a pathological effect and Torynurus convolutus was responsible for the death of one animal from the Belgian coast and three from the German coast. [less ▲]

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See detailSyndiotactic poly(methyl methacrylate) (sPMMA)-polybutadiene (PBD)-sPMMA triblock copolymers: synthesis, morphology, and mechanical properties
Yu, Jian Ming; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecules (1996), 29(19), 6090-6099

A series of syndiotactic poly(methyl methacrylate) (sPMMA)−polybutadiene (PBD)−sPMMA triblock copolymers, or MBM, have been successfully synthesized by using dilithium initiators (DLi's) based on the ... [more ▼]

A series of syndiotactic poly(methyl methacrylate) (sPMMA)−polybutadiene (PBD)−sPMMA triblock copolymers, or MBM, have been successfully synthesized by using dilithium initiators (DLi's) based on the diadduct of tert-butyllithium (t-BuLi) to either 1,3-bis(1-phenylethenyl)benzene (PEB) or m-diisopropenylbenzene (m-DIB). The efficiency of these DLi's in building up MBM triblock copolymers has been compared under the same experimental conditions, i.e., in a cyclohexane/diethyl ether mixture for the butadiene polymerization at room temperature and in a cyclohexane/THF mixture for the MMA polymerization at −78 °C. Although all the synthesized copolymers show a monomodal, symmetric, and very narrow molecular weight distribution, the MBM copolymers synthesized with the m-DIB/t-BuLi diadduct are pure triblocks and show a high tensile strength, in contrast to copolymers initiated by the PEB/t-BuLi diadducts that are of a lower tensile strength and contaminated by MB diblock copolymers. Solvent cast films of MBM prepared with the m-DIB/t-BuLi diadduct are two-phase materials as confirmed by DSC and dynamic mechanical analysis (DMA). Transmission electron microscopy (TEM) shows a spherical morphology at a low sPMMA content, that changes into a cylindrical and finally lamellar morphology upon increasing the sPMMA content. Phase separation is observed for MBM with M̄n of the sPMMA blocks as low as 6000. Dependence of tensile strength on copolymer structure and sample preparation has been studied. The smaller sPMMA molecular weight, M̄n(PMMA), required for high tensile strength depends on the PBD molecular weight, M̄n(PBD), e.g. 12 000 for Mn(PBD) = 36 000 and 6000 for Mn(PBD) = 80 000. The upper M̄n(PMMA) is ca. 20−25 000, whatever the Mn(PBD). The optimum tensile strength is observed for M̄n(PMMA) = 15 000, independently of Mn(PBD) in the studied range. As a rule, the tensile strength tends to level off and the elongation at break starts to decrease when the sPMMA content is increased beyond 35 wt %. At a constant sPMMA content, Mn(PBD) (>ca. 36 000) does not affect the ultimate tensile properties. [less ▲]

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See detailMicrodomain morphology analysis of block copolymers by atomic force microscopy with phase detection imaging
Leclère, Philippe; Lazzaroni, Roberto; Brédas, Jean-Luc et al

in Langmuir (1996), 12(18), 4317-4320

We use atomic force microscopy (AFM) with phase detection imaging (PDI) in order to study the surface microdomain morphology of thick (i.e., ca. 2 mm) films of triblock copolymers. We present here the ... [more ▼]

We use atomic force microscopy (AFM) with phase detection imaging (PDI) in order to study the surface microdomain morphology of thick (i.e., ca. 2 mm) films of triblock copolymers. We present here the results obtained on a poly(methyl methacrylate)-block-polybutadiene-block-poly(methyl methacrylate) (PMMA-b-PBD-b-PMMA) copolymer prepared by using a 1,3-diisopropenylbenzene (DIB)-based difunctional anionic initiator. Our data illustrate the interest of PDI for the elucidation of surface phase separation in block copolymers. We show that the surface of thick films studied by this new technique exhibits a two-phase structure corresponding to the two types of components. [less ▲]

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See detailBinding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair protein MSH2 and MSH3
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Current Biology (1996), 6(9), 1185-7

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See detailEffects of Schwann Cell Transplantation in a Contusion Model of Rat Spinal Cord Injury
Martin, Didier ULg; Robe, Pierre ULg; Franzen, Rachelle ULg et al

in Journal of Neuroscience Research (1996), 45(5), 588-597

Cultured Schwann cells were transplanted at various delays into a spinal cord contusion injury performed at low thoracic level in adult female rats. The Schwann cells were purified from the dorsal root ... [more ▼]

Cultured Schwann cells were transplanted at various delays into a spinal cord contusion injury performed at low thoracic level in adult female rats. The Schwann cells were purified from the dorsal root ganglia of adult syngeneic animals. the transplants were well tolerated, and the transplanted Schwann cells invaded the injured spinal cord. As quantified using video image analysis, the survival and growth of the transplanted cells were poor when the grafting procedure was performed 3-4 days after injury and very good when performed immediately or 10 days after injury, in which cases post-traumatic micro- and macrocavitation were strongly reduced. In animals grafted immediately after injury but not in animals grafted after 10 days, post-traumatic astrogliosis was much reduced. The Schwann cells transplanted area was invaded by numerous regenerating axons, the vast majority of which were, based on the neurotransmitter (CGRP and SP) profile, originating from dorsal root ganglion. No regeneration of the corticospinal tract as assessed after anterograde tracing or of descending aminergic fibers could be demonstrated. [less ▲]

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See detailPKS 1610-771: a highly reddened quasar?
Courbin, F.; Hutsemekers, Damien ULg; Meylan, G. et al

in The Messenger (1996), 85

Not Available

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See detailThe distribution of hot hydrogen atoms produced by electron and proton precipitation in the Jovian aurora
Bisikalo, D. V.; Shematovich, V. I.; Gérard, Jean-Claude ULg et al

in Journal of Geophysical Research (1996), 101

The energy distribution functions of nonthermal thermospheric hydrogen atoms are calculated for electron and proton precipitation in the Jovian aurora. A numerical model taking into account the production ... [more ▼]

The energy distribution functions of nonthermal thermospheric hydrogen atoms are calculated for electron and proton precipitation in the Jovian aurora. A numerical model taking into account the production, elastic and inelastic relaxation and transport processes for hot H atoms is developed. This model is based on a Monte Carlo solution of the nonlinear Boltzmann equation for hot H atoms produced by electron and proton impact on H and H[SUB]2[/SUB] and exothermic chemical reactions. The distribution functions show a much higher energy tail for proton than electron precipitation. It is shown that the steady state flux of hot atoms (E>=2 eV) is essentially isotropic. The peak and column hot H densities are about 3×10[SUP]5[/SUP] cm[SUP]-3[/SUP] and 1×10[SUP]14[/SUP] cm[SUP]-3[/SUP] for a 100 erg cm[SUP]-2[/SUP]s[SUP]-1[/SUP] precipitation combining hard (22 keV) and soft (0.2 keV) electrons mixed with a 10 erg cm[SUP]-2[/SUP]s[SUP]-1[/SUP] flux of soft (0.3 keV) protons. These column densities, coupled with the wide range of hot H atom energies, may play an important role in the formation of the Lyman alpha line profile. Multiple scattering in the wings of the Ly alpha line by the fast H atoms is shown to partly account for the broad Ly alpha profile observed in the Jovian aurora with the Hubble space telescope. [less ▲]

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See detailAcute Diverticulitis in Heart Transplant Recipients
Detry, Olivier ULg; Defraigne, Jean-Olivier ULg; Meurisse, Michel ULg et al

in Transplant International (1996), 9(4), 376-9

Immunosuppressed patients are susceptible to complicated diverticulitis, but reports of this complication are scarce in heart graft recipients. To estimate the prevalence of acute diverticulitis in heart ... [more ▼]

Immunosuppressed patients are susceptible to complicated diverticulitis, but reports of this complication are scarce in heart graft recipients. To estimate the prevalence of acute diverticulitis in heart graft recipients, we retrospectively reviewed the cases of diverticulitis in a series of 143 patients who underwent orthotopic heart transplantation in a period of 10 years. Six (4%) of these developed acute diverticulitis and required colectomy. All of them were male patients and were older than 50 years. Four patients underwent urgent laparotomy and colon resection with end colostomy (Hartmann procedure). The two other patients suffered from diverticulitis without generalized peritonitis and underwent laparoscopic sigmoidectomy with direct transanal end-to-end anastomosis. The postoperative outcomes of these six patients were satisfactory. As are other immunosuppressed patients, heart graft recipients are susceptible to diverticulitis. Early surgical management may be safe in well-compensated patients. [less ▲]

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See detailIn Vivo Expression of Interleukin-1 Beta (Il-1 Beta), Il-2, Il-4, Il-6, Tumour Necrosis Factor-Alpha and Interferon-Gamma in the Fetal Murine Thymus
Deman, J.; Van Meurs, M.; Claassen, E. et al

in Immunology (1996), 89(1), 152-7

Cytokines are known to play a role in T-cell lymphopoiesis as potent growth or differentiation factors, but many experiments focusing on their role in the thymus have been conducted only in vitro. We have ... [more ▼]

Cytokines are known to play a role in T-cell lymphopoiesis as potent growth or differentiation factors, but many experiments focusing on their role in the thymus have been conducted only in vitro. We have thus used frozen sections obtained from fetal thymuses of normal C57BL 6 mice to investigate by immunohistochemistry the presence of interleukin-1 beta (I4-1 beta), IL-2. IL-4. IL-6. interferon-7 (IFN-7) and tumour necrosis facor-alpha (TNF-alpha). The results reveal that apart from IL-2, which was not detected, all these cytokines display a time-dependent expression pattern in the normal fetal thymus. First, production of IL-4, IL-6 and TNF-alpha is detected around days 13 14; this is followed by a second wave on days 16 17, with a production of IL-1 beta, IL-4 and IL-6, and finally, just before birth (day 19), by a third wave of IL-1 beta, IL-4, IL-6, IFN-7 and TNF-alpha production. This supports the hypothesis that cytokines play a rote in T-cell lymphopoiesis. [less ▲]

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See detailInflammatory reaction and capillary leak syndrome related to cardiopulmonary bypass in neonates undergoing cardiac operations
Seghaye, Marie-Christine ULg; Grabitz, R. G.; Duchateau, J. et al

in Journal of Thoracic and Cardiovascular Surgery (1996), 112(3), 687-697

We studied the inflammatory reaction related to cardiopulmonary bypass in 24 neonates (median age 6 days) undergoing the arterial switch operation for simple transposition of the great arteries, with ... [more ▼]

We studied the inflammatory reaction related to cardiopulmonary bypass in 24 neonates (median age 6 days) undergoing the arterial switch operation for simple transposition of the great arteries, with respect to the development of postoperative capillary leak syndrome. Complement proteins, leukocyte count, tumor necrosis factor-α, and histamine levels were determined before, during, and after cardiopulmonary bypass. Additionally, protein movement from the intravascular into the extravascular space during cardiopulmonary bypass was assessed by the measurement of plasma concentrations of proteins with molecular weights ranging from 21,200 to 718,000. Capillary leak syndrome developed in 13 of the 24 neonates. Patients with capillary leak syndrome, as compared with those without, had preoperatively higher C5a levels (C5a, 3.0 ± 0.6 μg/L vs 0.9 ± 0.2 μg/L) (mean ± standard error of the mean) (p < 0.05) and higher leukocyte counts (leukocytes, 17.9 ± 2.1 x 10 3 cells/ml versus 11.7 ± 0.8 x 10 3 cells/ml) (p < 0.05), suggesting in these neonates a preoperative inflammatory state. Preoperative clinical and operative data were identical in both patient groups. Before cardiopulmonary bypass, serum protein concentrations were similar in all patients. Ten minutes after institution of cardiopulmonary bypass, protein concentrations fell to significantly lower values in patients with capillary leak syndrome than in those without: albumin (19% ± 1.5% vs 30% ± 6% of the prebypass value, p < 0.05), immunoglobulin G (17% ± 1.5% vs 29% ± 5.5%, p < 0.001), and α 2-macroglobulin (15% ± 1.2% vs 25% ± 4%, p < 0.02). During cardiopulmonary bypass, albumin concentrations remained significantly lower in patients with capillary leak syndrome than in those without, whereas hematocrit values were similar in both groups. During cardiopulmonary bypass, patients with capillary leak syndrome also had lower concentrations of complement proteins C3 and C4 but not C1 inhibitor. C3d/C3 ratio and C5a levels were similar in both patient groups. In contrast, histamine liberation during cardiopulmonary bypass was significantly more pronounced in patients with capillary leak syndrome than in those without (725.2 ± 396.7 pg/ml vs -54.1 ± 58.4 pg/ml, p < 0.05). Tumor necrosis factor-α levels after protamine administration were also significantly higher in patients with capillary leak syndrome (38.1 ± 10.0 pg/ml vs 15.3 ± 3.4 pg/ml, p < 0.05). Leukocyte count during and after cardiopulmonary bypass was similar in both patient groups. This study demonstrates increased protein leakage as early as 10 minutes after initiation of cardiopulmonary bypass in patients having clinical signs of postoperative capillary leak syndrome. Patients with capillary leak syndrome displayed more pronounced histamine liberation and tumor necrosis factor-α liberation than patients without capillary leak syndrome, suggesting a relationship between cardiopulmonary bypass-related inflammatory reaction and perioperative capillary damage. A preoperative inflammatory state in patients with capillary leak syndrome could have enhanced the inflammatory response to cardiopulmonary bypass. [less ▲]

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See detailThe non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia Coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure
Goffin, Colette ULg; Fraipont, Claudine ULg; Ayala, Juan et al

in Journal of Bacteriology (1996), 178(18), 5402-5409

The ftsI-encoded multimodular class B penicillin-binding protein 3 (PBP3) is a key element of the cell septation machinery of Escherichia coli. Altered ftsI genes were overexpressed, and the gene products ... [more ▼]

The ftsI-encoded multimodular class B penicillin-binding protein 3 (PBP3) is a key element of the cell septation machinery of Escherichia coli. Altered ftsI genes were overexpressed, and the gene products were analyzed with respect to the level of production, stability, penicillin affinity, and cell septation activity. In contrast to the serine beta-lactamases and low-molecular-mass PBPs which are autonomous folding entities, the S-259-to-V-577 penicillin-binding module of M-1-to-V-577 PBP3 lacks the amino acid sequence information for correct folding. The missing piece of information is provided by the associated G-57-to-E-258 non-penicillin-binding module which functions as a noncleaved, pseudointramolecular chaperone. Key elements of the folding information reside within the motif 1-containing R-60-to-W-110 polypeptide segment and within G-188-to-D-197 motif 3 of the n-PB module. The intermodule interaction is discussed in the light of the known three-dimensional structure (at 3.5-A [0.35-nm] resolution) of the analogous class B PBP2x of Streptococcus pneumoniae (S. Pares, N. Mouz, Y. Petillot, R. Hakenbeck, and O. Dideberg, Nature Struct. Biol. 3:284-289, 1996). Correct folding and adoption of a stable penicillin-binding conformation are necessary but not sufficient to confer cell septation activity to PBP3 in exponentially growing cells. The in vivo activity of PBP3 also depends on the M-1-to-E-56 amino-terminal module which encompasses the cytosol, the membrane, and the periplasm and which functions as a noncleaved pseudo-signal peptide. [less ▲]

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See detailThrombolyse coronaire--actualisation
Legrand, Victor ULg

in Revue Médicale de Liège (1996), 51(9), 571-5

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See detailLocalization of Testosterone-Sensitive and Sexually Dimorphic Aromatase-Immunoreactive Cells in the Quail Preoptic Area
Balthazart, Jacques ULg; Tlemcani, O.; Harada, N.

in Journal of Chemical Neuroanatomy (1996), 11(3), 147-71

The distribution of aromatase-immunoreactive cells was studied in the medial preoptic nucleus of male and female quail that were sexually mature and gonadally intact, or gonadectomized, or gonadectomized ... [more ▼]

The distribution of aromatase-immunoreactive cells was studied in the medial preoptic nucleus of male and female quail that were sexually mature and gonadally intact, or gonadectomized, or gonadectomized and treated with testosterone. The study first confirmed the existence of a significant difference in the number of aromatase-immunoreactive cells between males and females (males > females) and the marked effect of castration and testosterone treatment which, respectively, decrease and restore the number of these cells. An analysis of the distribution in space of this neurochemically defined cell population was also carried out. This study revealed that castration does not uniformly decrease the density of aromatase-immunoreactive cells, but local increases are observed in an area directly adjacent to the third ventricle. A number of new sex differences in the organization of the medial preoptic nucleus and its population of aromatase cells have, in addition, been identified. The density of aromatase-immunoreactive cells is not higher in males than in females throughout the nucleus, but a higher density of immunoreactive cells is present in the ventromedial part of the nucleus in females as compared to males. In addition, the cross-sectional area of the nucleus as defined by the population of aromatase-immunoreactive cells is larger in males than in females in its rostral part and its shape is more elongated in the dorso-ventral direction in females than in males. Some of these differences (e.g. higher density of ARC-ir cells in the ventromedial part of the female POM, shape of the nucleus) appear to be organizational in nature, because they are still present in birds exposed to the same endocrine conditions during adult life (e.g. gonadectomized and treated with a same dose of testosterone). This conclusion should now be tested by experiments manipulating the endocrine environment of quail embryos. The anatomical heterogeneity of the medial preoptic nucleus revealed by this study also suggests a functional heterogeneity and the specific roles of the medial and lateral parts of the nucleus should also be investigated. [less ▲]

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See detailNeurotrophic Factors: Past and Future
Moonen, Gustave ULg; Malgrange, Brigitte ULg; Rigo, Jean Michel et al

in Acta Neurologica Belgica (1996), 96(3), 203-18

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See detailTyping of Bovine Attaching and Effacing Escherichia Coli by Multiplex in Vitro Amplification of Virulence-Associated Genes
China, B.; Pirson, V.; Mainil, Jacques ULg

in Applied and Environmental Microbiology (1996), 62(9), 3462-3465

Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by ... [more ▼]

Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method. [less ▲]

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See detailNaevocyte Triggering by Recombinant Human Growth Hormone
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Nikkels, Arjen ULg et al

in Journal of Pathology (The) (1996), 180(1), 74-79

The influence of growth hormone and insulin-like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is ... [more ▼]

The influence of growth hormone and insulin-like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is administered to children of short stature, the growth of melanocytic naevi is boosted. This study was conducted on 56 hGH-triggered naevi and nine similar lesions excised before or after hGH therapy for hypopituitarism and Turner's syndrome. A series of 40 naevi excised from age-matched healthy children served as controls. Atypicality of naevocytes was investigated using image analysis, AgNOR counts, immunohistochemistry (HMB-45, NKI-C3, Ki-67, anti-bcl-2-oncoprotein), and DNA flow cytometry. The data associate hGH treatment with anisokaryosis and increased AgNOR and Ki-67 counts in naevocytes. The same cells also show abnormal patterns of HMB-45 immunolabelling. These indications of naevocyte activation were not suggestive of malignant transformation. hGH-triggered melanocytomas should be added to the list of atypical melanocytic naevi. The long-term evolution of these lesions remains unknown and the potential risk of malignant transformation awaits careful evaluation. [less ▲]

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See detailCaractérisation métabolique cérébrale et neuropsychologique des syndromes parkinsoniens
Salmon, Eric ULg; Van der Linden, Martial ULg; Garraux, Gaëtan ULg et al

in Revue Médicale de Liège (1996), 51(9), 566-70

S'il existe des signes neurologiques caractéristiques de la maladie de Parkinson, l'expérience clinique nous apprend que l'évolution individuelle des patients parkinsoniens est fort variable, et des ... [more ▼]

S'il existe des signes neurologiques caractéristiques de la maladie de Parkinson, l'expérience clinique nous apprend que l'évolution individuelle des patients parkinsoniens est fort variable, et des études anatomopathologioques révèlent que le diagnostic clinique d'un syndrome parkinsonien est parfois difficile à poser. Nous revoyons dans cet article les diagnostics différentiels des syndromes parkinsoniens, et nous discutons l'utilité de la neuroimagerie fonctionnelle et du bilan neuropsychologique pour essayer de mieux comprendre la présentation clinique. [less ▲]

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See detailAnterior cingulate dysfunction in presenile dementia due to progressive supranuclear palsy
Salmon, Eric ULg; Meulemans, Thierry ULg; Van der Linden, Martial ULg et al

in Acta Neurologica Belgica (1996), 96(3), 247-253

We report neuropsychological and neuroimaging investigations performed in a patient suffering from presenile onset degenerative dementia and subsequent mild extrapyramidal and pyramidal signs. Early ... [more ▼]

We report neuropsychological and neuroimaging investigations performed in a patient suffering from presenile onset degenerative dementia and subsequent mild extrapyramidal and pyramidal signs. Early neuropsychological testing revealed frontal lobe dysfunction. Neuroanatomical pictures were not contributive. After four years of evolution, the clinical pattern was consistent with progressive supranuclear palsy. Statistical parametric mapping analysis of functional imaging revealed a highly significant metabolic impairment in the anterior cingulate gyrus, that might be a key feature of subcortico-frontal dementia in progressive supranuclear palsy. [less ▲]

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See detailTwo-component spreading phenomena: Why the geometry makes the criticality
Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Physical Review. E : Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics (1996), 54(3), 3006-3008

We have numerically and theoretically investigated a simple model for two-component spreading phenomena in two different growth geometries (i.e., spreading confined in a half space and spreading in a free ... [more ▼]

We have numerically and theoretically investigated a simple model for two-component spreading phenomena in two different growth geometries (i.e., spreading confined in a half space and spreading in a free space). The criticality of the domain substructures unexpectedly depends on the considered geometry. This is understood by simple arguments of domain-wall particle diffusion and annihilation. We derive a relationship between the critical exponents chi and alpha for domain-wall spatial distributions in different geometries. The latter relationship is numerically verified in two, three and four dimensions. [less ▲]

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See detailFactor Xiiia-Positive Dendrocytes and Proliferative Activity of Cutaneous Cancers
Pierard-Franchimont, Claudine ULg; Arrese Estrada, Jorge ULg; Nikkels, Arjen ULg et al

in Virchows Archiv : An International Journal of Pathology (1996), 429(1), 43-48

Factor XIIIa-positive dendrocytes present at the periphery and inside epithelial neoplasms are an heterogeneous group of cells. They are subsets of mesenchymal cells, cancer-associated macrophages and ... [more ▼]

Factor XIIIa-positive dendrocytes present at the periphery and inside epithelial neoplasms are an heterogeneous group of cells. They are subsets of mesenchymal cells, cancer-associated macrophages and antigen-presenting cells. Factor XIIIa, other tissue transglutaminases, alpha 2-macroglobulin and tumour necrosis factor-alpha represent a complex network of mediators influencing tumour progression in the skin. In the present study we searched for the presence of dendrocytes and alpha 2-macroglobulin deposits inside and in the vicinity of cutaneous carcinomas (90 basal cell carcinomas and 46 squamous cell carcinomas) and malignant melanomas (69 primary and 28 metastatic tumours). We also studied the proliferation of the same neoplasms by Ki-67 immunohistochemistry. Dendrocytes were numerous, abutting on and infiltrating most basal cell carcinomas and thin malignant melanomas. In contrast, they were present in only low numbers or even absent in thick primary malignant melanomas and in their metastases. They appeared unmodified around squamous cell carcinomas compared with the surrounding skin. Extracellular deposits of alpha 2-macroglobulin were often found in locations where dermal dendrocytes were numerous. No correlation was found between the Ki-67 indices of carcinomas and the density of peritumoral dendrocytes. In contrast, negative relationships were found between the Ki-67 indices and the number of dendrocytes present inside basal cell carcinomas and thin malignant melanomas. This study has yielded circumstantial evidence to link the density of factor XIIIa-positive dendritic cells and a low proliferative rate of neoplastic cells in basal cell carcinomas and malignant melanomas. [less ▲]

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See detailLaboratory and in situ grazing rates of freshwater rotifers and their contribution to community grazing rates
Gosselain, V.; Joaquim-Justo, Célia ULg; Viroux, L. et al

in Archiv für Hydrobiologie. Supplementband, Large rivers (1996), 13(1-4), 353-361

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See detailSimulations of the kinetic growth of YBa2Cu307-delta grains
Cloots, Rudi ULg; Vandewalle, Nicolas ULg; Ausloos, Marcel ULg

in Journal of Crystal Growth (1996), 166(1-4), 816-819

Using simple kinetic and geometric arguments, we have developed a computer model which simulates the growth of melt-textured YBa2Cu307-delta grains along the a-b planes. A chemical bonding anisotropy ... [more ▼]

Using simple kinetic and geometric arguments, we have developed a computer model which simulates the growth of melt-textured YBa2Cu307-delta grains along the a-b planes. A chemical bonding anisotropy along the main lattice directions is taken into account by the model. We have also considered the presence of Y2BaCu05 particles becoming trapped or not in the solidifying YBa2Cu307-delta front. The model simulates the fomation of 211 segregation patterns in the 123 matrix. An interpretation of these is attempted in terms of trapping anisotropy due to intrinsic crystallographic anisotropy. [less ▲]

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See detailUse of Transdermal Oestradiol (Estraderm Tts) in Hypertensive Postmenopausal Women
Foidart, Jean-Michel ULg

in British Journal of Clinical Practice. Supplement (1996), 86

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