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See detailDissemination of Catabolic Plasmids among Desiccation-Tolerant Bacteria in Soil Microcosms
Weekers, Frederic; Rodriguez, Christian; Jacques, Philippe et al

in Applied Biochemistry and Biotechnology (2001), 91-93(Spring), 219-32

The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trichloroethene ... [more ▼]

The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trichloroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. This plasmid had a narrow host range. Pseudomonas putida strain C8S3 was used as the inoculum in a second approach. It bore a broad host range conjugative plasmid harboring a natural transposon, RP4::Tn4371, responsible for biphenyl and 4-chlorobiphenyl degradation pathways. The inoculating population slowly decreased from its original level (10(6) colony-forming units [CFU]/g of dry soil) to approx 3 x 10(2) CFU/g of dry soil after 3 wk. Transconjugant populations degrading biphenyl appeared in constant humidity soil (up to 2 x 10(3) CFU/g) and desiccating soil (up to 10(4) CFU/g). The feasibility of plasmid dissemination as a bioaugmentation technique was demonstrated in desiccating soils. The ecologic significance of desiccation in bioaugmentation was demonstrated: it upset the microbial ecology and the development of transconjugants. [less ▲]

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See detailDiscretization Schemes for Hybrid Methods
Geuzaine, Christophe ULg; Tarhasaari, T.; Kettunen, L. et al

in IEEE Transactions on Magnetics (2001), 37(5), 3112--3115

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See detailPex, Analytical Tools For Pdb Files. I. Gf-Pex: Basic File To Describe A Protein
Thomas, Annick ULg; Bouffioux, O.; Geeurickx, D. et al

in Proteins-Structure Function and Genetics (2001), 43(1), 28-36

Pex are created to extract numeric and string descriptions of protein structures from PDB files. This concerns covalent bond lengths and angles, secondary structures, residues in interaction, H-bond ... [more ▼]

Pex are created to extract numeric and string descriptions of protein structures from PDB files. This concerns covalent bond lengths and angles, secondary structures, residues in interaction, H-bond lengths and geometry, etc. Several kinds of Pex are generated: (1) general feature (GF-Pex); (2) H-bond (H-Pex); and (3) accessible surface (AS-Pex) and force potential (FP-Pex). We describe the general principles of Pex and detail the GF-Pex files. Using the GF-Pex of 131 proteins, we analyze the mean residue frequencies, the straight phi/psi distribution and the major kinds of secondary structures in proteins. Thomas et al. (this issue) analyzes the main chain H-bonds in those proteins. The GF-Pex and H-Pex files of the 131 proteins can be downloaded from the CBMN site (http://www.fsagx.ac.be/bp/). [less ▲]

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See detailSuboptimal Enhancer Sequences Are Required For Efficient Bovine Leukemia Virus Propagation In Vivo: Implications For Viral Latency
Merezak, C.; Pierreux, C.; Adam, E. et al

in Journal of Virology (2001), 75(15),

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See detailFactors affecting hematopoietic recovery after autologous peripheral blood progenitof-cell transplantation in aggressive non-Hodgkin's lymphoma : a prospective study of 123 patients
Langouet, Anne-Marie; Brice, Pauline; Simon, Danielle et al

in Hematology Journal : The Official Journal of the European Haematology Association (2001), 2

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See detailEnhanced tropospheric HCN columns above Kitt Peak during the 1982-1983 and 1997-1998 El Nino warm phases
Rinsland, Curtis P.; Goldman, Aaron; Zander, Rodolphe ULg et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (2001), 69(1), 3-8

Free tropospheric HCN columns have been derived from infrared solar spectra recorded with the National Solar Observatory Fourier transform spectrometer on Kitt Peak, Arizona (31.9ºN latitude, 111.6ºW ... [more ▼]

Free tropospheric HCN columns have been derived from infrared solar spectra recorded with the National Solar Observatory Fourier transform spectrometer on Kitt Peak, Arizona (31.9ºN latitude, 111.6ºW longitude, 2.09 km altitude) between May 1978 and May 2000. The time series show up to a factor of 2.4 enhancement during the strong El Ninos of 1982-1983 and 1997-1998, the most intense since 1970. The observations provide confirmation that HCN is a sensitive tracer of biomass burning emissions transported to the free troposphere. No statistically significant long-term trend in the HCN-free tropospheric column has been detected over the 22-year measurement period. The results illustrate the importance of long-term spectroscopic measurements for quantifying climate and atmospheric chemistry-related atmospheric changes. [less ▲]

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See detailAmputations in Diabetic Patients: A Plea for Footsparing Surgery
Van Damme, Hendrik ULg; Rorive, Marcelle ULg; Martens De Noorthout, B. M. et al

in Acta Chirurgica Belgica (2001), 101(3, May-Jun), 123-9

The authors observed a rather high rate of primary major amputation (above-knee or below-knee) performed for diabetic foot problems as well as an important revision rate for minor amputations (forefoot or ... [more ▼]

The authors observed a rather high rate of primary major amputation (above-knee or below-knee) performed for diabetic foot problems as well as an important revision rate for minor amputations (forefoot or toe) in diabetics. They reviewed their experience in order to compare it with more recent data from the literature, pleading for foot-sparing surgery. From 1993 to 1998, 186 amputations were performed on 146 diabetic patients. The cause of foot ulcers was neuropathy in 43 of them (51 episodes of diabetic foot problems) while in the remaining 103 patients (135 episodes of diabetic foot problems), diabetic macroangiopathy (absent ankle pulses) was on cause. For neuropathic foot problems, amputations were almost minor, resulting in a limb salvage rate of 90%. Only five of these patients (12%) had primary major limb amputation versus 43 of the dysvascular patients (42%). The reasons for major amputation by first intention were extensive tissue loss, intractable infection or non-reconstructible occlusive vessel disease, as judged by the surgeon. A foot-sparing surgery was attempted in 92 dysvascular cases. In only 44 of them, a preliminary vascular repair was performed. Twenty eight percent of the primary toe amputations and 24% of the forefoot amputations required secondary revision to a more proximal level. Minor amputations in case of diabetic neuropathy were characterized by a more favourable outcome: only 14% of the toe and 9% of the forefoot amputations failed. During follow-up, only 63% of the major amputations regained an autonomic walking capability with their prosthesis. Wound healing problems in diabetic foot are mainly due to infection and poor tissue perfusion. An aggressive control of the infection and distal revascularization of calf- or foot arteries, whenever possible, could improve the results of diabetic foot surgery. The poor functional recovery after major amputation (only 63% autonomic gait with limb prosthesis) argues for foot-sparing surgery whenever possible. [less ▲]

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See detailTrophic diversiy within the eastern Weddell Sea amphipod community
Dauby, Patrick ULg; Scailteur, Yves; De Broyer, Claude

in Hydrobiologia (2001), 443

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See detailSedimentology of the Bieumont Member : influence of the Lion Member Carbonate mounds (Frasnian, Belgium) on their sedimentary environment
Humblet, M.; Boulvain, Frédéric ULg

in Geologica Belgica (2001), 3(1-2), 97-118

This is a sedimentological study of Middle Frasnian Bieumont Member fore-reef and off-reef carbonate sediments exposed in the Chimay-Couvin region (Belgium). It is based on four stratigraphic sections ... [more ▼]

This is a sedimentological study of Middle Frasnian Bieumont Member fore-reef and off-reef carbonate sediments exposed in the Chimay-Couvin region (Belgium). It is based on four stratigraphic sections: the Lompret quarry, the Frasnes railway section, the Leus quarry and southern parts of the Lion quarry. The Lompret section is here described for the first time and represents the main focus of this work. The Bieumont Member consists of a bedded sequence of argillaceous and bioclastic limestone. At Lompret, the Member is exposed with a thickness of 48 m (the basal contact is not exposed). The Member consists of 6 lithological units (in stratigraphic order) : 1. argillaceous limestone with episodic intercalations of reef debris (distal reef talus), 2. an alternation of marl and argillaceous limestone (basinal background sediment), 3. thickly-bedded bioclastic limestone (channel deposits), 4. a sequence of argillaceous limestone (inter-channel deposits), 5. bioclastic limestone with sporadic occurrence of framestone (proximal fore-reef deposits) and 6. fine-grained, bioclastic limestone interbedded with prominent layers of reef debris (distal fore-reef deposits). Within unit 5, an isolated reef block transported down the paleoslope is present. Microfacies analysis revealed 16 microfacies units which together with the lithological subdivisions were used to reconstruct the dynamic sedimentary history of the Bieumont Member at Lompret. There are two orders of depositional rhythmicity. The reef growth cycle starts with the first significant influx of reef-related facies at the beginning of unit 3 and from thereon is well differentiated from the Bieumont Member stratotype which largely consists of lithologies equivalent to units 1 and 2. Progradation was directed to the South and related shallowing-upward conditions culminated during the deposition of unit 5. On a lower scale, rhythmic bedding within units 2 and 4 could be related to orbitally forced cycling. The vertical and lateral variations of facies within the Bieumont Member were used to reconstruct a depositional model for the Lompret area. In addition, the documentation of facies variation on a regional scale was enhanced by using data from the Focant borehole and geological surveys of Han-sur-Lesse and Barvaux regions. [less ▲]

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See detailIrreversible magnetization under rotating fields and lock-in effect on a ErBa2Cu3O7-delta single crystal with columnar defects
Avila, M. A.; Civale, L.; Silhanek, Alejandro ULg et al

in Physical Review. B : Condensed Matter (2001), 64(14),

We have measured the irreversible magnetization (M-i) of an ErBa2Cu3O7-delta single crystal with columnar defects (CD), using a technique based on sample rotation under a fixed magnetic field H. This ... [more ▼]

We have measured the irreversible magnetization (M-i) of an ErBa2Cu3O7-delta single crystal with columnar defects (CD), using a technique based on sample rotation under a fixed magnetic field H. This method is valid for samples whose magnetization vector remains perpendicular to the sample surface over a wide angle range-which is the case for platelets and thin films-and presents several advantages over measurements of M-L(H) loops at fixed angles. The resulting M-i(Theta) curves for several temperatures show a peak in the CD direction at high fields. At lower fields, a very well defined plateau indicative of the vortex lock-in to the CD develops. The H dependence of the lock-in angle phi (L) follows the H-1 theoretical prediction, while the temperature dependence is in agreement with entropic smearing effects corresponding to long range vortex-defects interactions. [less ▲]

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See detailAuto/biographie. Le dispositif Mallarmé/Verlaine
Durand, Pascal ULg

in Littératures (2001), 44(printemps), 97-119

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See detailEffect of conventional controlled-rate freezing and vitrification on quality of bovine blasotcysts produced in vitro
Kaidi, Safia; Bernard, S.; Lambert, Philippe ULg et al

in Biology of Reproduction (2001), 65

This study compares the effects of conventional controlled-rate freezing and vitrification on the morphology and metabolism of in vitro-produced bovine blastocysts. Day 7 expanded blastocysts cultured in ... [more ▼]

This study compares the effects of conventional controlled-rate freezing and vitrification on the morphology and metabolism of in vitro-produced bovine blastocysts. Day 7 expanded blastocysts cultured in synthetic oviduct fluid with 5% fetal calf serum were frozen in 1.36 M glycerol, 0.25 M sucrose or vitrified in 25% glycerol, 25% ethylene glycol. Cell alterations and in vitro development were evaluated immediately after thawing or after 72 h. The effect of cryopreservation on inner cell mass and trophectoderm (TE) cell number as well as glucose, pyruvate, and oxygen uptakes, and lactate release by blastocysts were evaluated. Immediately after thawing, blastocysts showed equivalent cell membrane permeabilization after both cryopreservation procedures, while alterations in nuclear staining were more frequent in vitrified embryos. After culture, similar survival and hatching rates were observed. Both procedures decreased cell number immediately after thawing and after 72 h. However, the number of TE cells was lower in frozen embryos than in vitrified ones. In relation to this, frozen blastocysts showed a decrease in glucose, pyruvate, and oxygen uptake, although those parameters were not altered in vitrified embryos. An increased glycolytic activity was also observed in frozen embryos, indicating a stress response to this procedure. [less ▲]

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See detailPex, Analytical Tools For Pdb Files. II. H-Pex: Noncanonical H-Bonds In Alpha-Helices
Thomas, Annick ULg; Benhabiles, N.; Meurisse, R. et al

in Proteins-Structure Function and Genetics (2001), 43(1), 37-44

We use the H-Pex (Thomas et al., this issue) to analyze the main chain interactions in 131 proteins. In antiparallel beta-sheets, the geometry of the N...O bond is: median N...O distances, 2.9 SA, C==O ... [more ▼]

We use the H-Pex (Thomas et al., this issue) to analyze the main chain interactions in 131 proteins. In antiparallel beta-sheets, the geometry of the N...O bond is: median N...O distances, 2.9 SA, C==O...N angles at 154 degrees and the C alpha--C==O...H angles are dispersed around 3 degrees. In some instances, the other side of the C==O axis is occupied by a HC alpha. As recently supported by Vargas et al. (J Am Chem Soc 2000;122:4750-4755) C alpha H...O and NH...O could cooperate to sheet stability. In alpha-helices, the main chain C==O interact with the NH of their n + 4 neighbor on one side, and with a C beta H or C gamma H on the other side. The median O...N distance (3.0 A) and C==N angle (147 degrees) suggest a canonical H-bond, but the C alpha--C==O...H dihedral angle invalidates this option, since the hydrogen attacks the oxygen at 122 degrees, i.e., between the sp(2) and pi orbitals. This supports that the H-bond is noncanonical. In many instances, the C gamma H or the C beta H of the n + 4 residue stands opposite to the NH with respect to the oxygen. Therefore, we propose that, in alpha-helices, the C gamma H or C beta H and the NH of the n + 4 residue hold the oxygen like an electrostatic pincher. [less ▲]

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See detailHelioseismic Tests of the New Los Alamos LEDCOP Opacities
Neuforge-Verheecke, C.; Guzik, J. A.; Keady, J. J. et al

in Astrophysical Journal (2001), 561(1), 450-454

We compare the helioseismic properties of two solar models, one calibrated with the OPAL opacities and the other with the recent Los Alamos LEDCOP (Light Element Detailed Configuration Opacity) opacities ... [more ▼]

We compare the helioseismic properties of two solar models, one calibrated with the OPAL opacities and the other with the recent Los Alamos LEDCOP (Light Element Detailed Configuration Opacity) opacities. We show that, in the radiative interior of the Sun, the small differences between the two sets of opacities (up to 6% near the base of the convection zone) lead to noticeable differences in the solar structure (up to 0.3% in sound speed), with the OPAL model being the closest to the helioseismic data. More than half of the difference between the two opacity sets results from the interpolation scheme and from the relatively widely spaced temperature grids used in the tables. The remaining 3% intrinsic difference between the OPAL and the LEDCOP opacities in the radiative interior of the Sun is well within the error bars on the opacity calculations resulting from the uncertainties on the physics. We conclude that both the OPAL and LEDCOP opacities produce solar models in close agreement with helioseismic inferences, but discrepancies still persist at the level of 0.6% between the calculated and inferred sound speed in the radiative interior of the Sun. [less ▲]

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See detailLong Term Carbon Dioxide Exchange Above A Mixed Forest In The Belgian Ardennes
Aubinet, Marc ULg; Chermanne, B.; Vandenhaute, M. et al

in Agricultural and Forest Meteorology (2001), 108(4),

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See detailNew lithostratigraphical, sedimentological, mineralogical and palaeontological data on the Mesozoic of Belgian Lorraine: a progress report
Boulvain, Frédéric ULg; Belanger, I.; Delsate, D. et al

in Geologica Belgica (2001), 3(1-2), 3-33

A multidisciplinary study of some extensively cored boreholes together with the new 1/25.000 geological mapping of wallonia lead us to propose a new lithostratigraphic canvas for belgian lorraine. This ... [more ▼]

A multidisciplinary study of some extensively cored boreholes together with the new 1/25.000 geological mapping of wallonia lead us to propose a new lithostratigraphic canvas for belgian lorraine. This area is located on the n-e border of the paris basin, south of the ardennes; the studied stratigraphic interval covers the keuper to the toarcian. Each of the newly defined units is sedimentologicaly interpreted on the basis of macroscopic and microscopic observations. Detailed palynological and claystratigraphical analyses have been performed, providingadditional stratigraphical, palaeoecological and sedimentological data. Moreover, a first sequence stratigraphic interpretation is proposed for this highly transgressive sedimentary body. The habay formation (conglomerates and red mudstones) is a fluvial unit, with immature channel conglomerates and paleosoils; the attert formation (dolomitic marls with gypsum and pseudomorphs) exhibites an evaporitic trend. The Mortinsart formation (sands and marls) corresponds to a restricted marine unit, evolving towards an alluvial plain (levallois member). The second cycle begins with the Jamoigne Formation (bioturbated marls and limestones), a marine subtidal restricted unit, evolving towards a more sandy sedimentary body (Metzert Member). The base of the third cycle corresponds to the rest of the Luxemburg Formation, comprising a succession of sandy waves. The Ethe Formation (laminar mudstones and marls) marks a deepening of the basin and the outset of open marine dysaerobic conditions. The Aubange Formation (bioturbated marls with sandstones and limestones) is characterized by the reappearance of a normal benthic fauna. The Grandcourt Formation (laminar mudstones and marls) marks a return to open marine dysaerobic conditions. The Mont-Saint-Martin Formation (marls, sandy marls and oolitic ironstone) is a highly regressive unit, while the Longwy Formation (limestones) marks the initiation of a carbonate platform. [less ▲]

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See detailA Parametric Distance Function Approach for Malmquist Productivity Index Estimation
Fuentes, Hugo J.; Grifell-Tatjé, Emili; Perelman, Sergio ULg

in Journal of Productivity Analysis (2001), 15

Malmquist indexes of productivity are generally estimated using index number techniques or non-parametric frontier approaches. The aim of this paper is to showthat Malmquist indexes can be estimated in a ... [more ▼]

Malmquist indexes of productivity are generally estimated using index number techniques or non-parametric frontier approaches. The aim of this paper is to showthat Malmquist indexes can be estimated in a similar way using parametric-deterministic or parametric-stochastic frontier approaches. To allow a multi-output multi-input technology and for technical change in production, we adopt an output distance function which is specified in a translog form. We then show that using the estimated parameters, several radial distance functions can be calculated and combined in order to estimate and decompose the productivity index. Finally, this approach is applied to a panel of Spanish insurance companies. The main results confirm those generally obtained for financial services: very low rates of growth and technical change in spite of a rapid deregulation process and expansion of activity. [less ▲]

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See detailImidazolopyrazinones as potential antioxydants
Devillers, Ingrid; Dive, Georges ULg; De Tollenaere, Catherine et al

in Bioorganic & Medicinal Chemistry Letters (2001), 11(17), 2305-2309

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See detailIntroduction of the multivariate Dale model in genetic association studies.
Van Steen, Kristel ULg; Tahri, N.; Molenberghs, G.

in American Journal of Human Genetics (2001), 69(4), 1289

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See detailKenneth Koch, New Addresses
Delville, Michel ULg

in Verse (2001)

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See detailThe effects of JL 13, a pyridobenzoxazepine with potential atypical antipsychotic activity, in animal models for schizophrenia
Ellenbroek, Bart; Liégeois, Jean-François ULg; Bruhwyler, Jacques et al

in Journal of Pharmacology and Experimental Therapeutics (2001), 298

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See detailPour une télématique de service public
Rondeaux, Giseline ULg; Schoenaers, Frédéric ULg

in Revue de Gestion (2001), (printemps),

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See detailLa fin de la vie en médecine intensive
Ferdinande, P.; Berré, J.; Colardyn, F. et al

in Réanimation (2001), 10

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See detailThe Composite Genome Of The Legume Symbiont Sinorhizobium Meliloti
Galibert, Francis; Finan, Turlough M.; Long, Sharon R. et al

in Science (2001), 293(5530),

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See detailMeningeal inflammatory pseudotumour: a case report.
Gollogly, L.; Sadzot, Bernard ULg; Lejeune, Jean-Luc ULg et al

in Acta Neurologica Belgica (2001), 101(2), 116-20

We report the case of a meningeal inflammatory pseudotumour occurring in a 23-year-old male presenting with focal seizures and headaches. Brain imaging techniques showed a 3.5 cm left parietal meningeal ... [more ▼]

We report the case of a meningeal inflammatory pseudotumour occurring in a 23-year-old male presenting with focal seizures and headaches. Brain imaging techniques showed a 3.5 cm left parietal meningeal tumour. Histology of the surgical specimen showed a dense lymphoid infiltrate permeating the dura mater and leptomeninges, consisting of a predominant polyclonal B cell population as confirmed by immunophenotyping and genotyping. Cultures of serum, CSF, and surgical specimen were negative and there was no serological evidence of a systemic dysimmune disease. The postoperative course was complicated by an episode of brain oedema resolving under steroid therapy. The patient, free from all medication, is asymptomatic at 3 years of follow-up. We discuss previously published cases and the nosology of intracranial inflammatory pseudotumours. [less ▲]

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See detailApplication and processing of geophysical images for mapping faults
Demanet, Donat; Renardy, François; Pirard, Eric ULg et al

in Computers & Geosciences (2001), 29(7), 1031-1037

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See detailDifferences in glucosinolate degradation products related to aphid and Brassicaceae host plant myrosinases
Francis, Frédéric ULg; Wathelet, Jean-Paul ULg; Lognay, Georges ULg

in GCIRC Bulletin (2001)

Studies on chemical ecology of host plant selection by herbivores were largely carried out to orplain the diversity of secondary plant chemicals and host specialisation of herbivores. The glucosinolate ... [more ▼]

Studies on chemical ecology of host plant selection by herbivores were largely carried out to orplain the diversity of secondary plant chemicals and host specialisation of herbivores. The glucosinolate - isothiocyanate system in Brassicaceae plants was considered as a plant defence and was known to influence the feeding behaviour of phytophagous insects such as the cabbage aphid, Brevicoryre brassicae. Purified m5nosinases from the latter and from white mustard, Sinqis alba, were used to hydrolyse two glucosinolate compounds. Identification of the degradation products, mainly isothiocyanates, was perficrmed by gas chromatography - mass spectromeûy. While sinigdn hydrolysis gave identical isothiocyanates for insect and plant enzymes, B. brassicae myrosinase showed a particular activity toward sinalbin A lost of an hydrory group was obsen'red for the two latter substrates related products when compared to the S. alba enzymatic specificity. Aphid and plant myrosinases have different properties and specificities which can be discussed in relation to a co-evolution approach. [less ▲]

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See detailRappresentazioni e pratiche Alimentari in Adolescenza. I risultati preliminari di un’analisi del contenuto delle Interviste semistrutturate con il Programma Alceste
D'Amore, Salvatore ULg

in Eating and Weight Disorders [=EWD] (2001), 6(2), 15

Many studies have analysed psychopathological dimensions of relationship that adolescent have with food and body. But few researches have focused attention on links between psychosocial dimensions such as ... [more ▼]

Many studies have analysed psychopathological dimensions of relationship that adolescent have with food and body. But few researches have focused attention on links between psychosocial dimensions such as social representations (Moscovici, S. 1961; Lalhou, S.1995) and adolescent typical practices of eating (with family and peers) and body (diet and sport). This research is aimed to describe and to analyse representations and practices of eating and body of 60 adolescents (30 females, 30 males) equally distributed on high and low social class aged from 14 to 16 years. Hypothesis supposes changes of eating and body representations and practices according to sexe and social class of subjects. A multimethodogical approach was adopted. Data were collected in Avril 1999 in two roman colleges. Qualitative and quantitative instruments were administered in this order: a) associative network (words association), b) semi-structured interviews (18 questions about eating and body ideas and practices), c) body drawing, d) eating family drawing, e) questionnaires (Eating Attitudes Test, Eating Disorders Inventory 2). A preliminary interviews content analysis with ALCESTE (Reinert, M.1993) draw out 8 discourse classes. Four classes are linked to eating representations and practices: a) Eating Family Practice (16,85 %); b) Eating with Friends (8, 61%); c) Eating quantity and preferences (15, 97%); d) Daily meals organisation (7,40%). Others four classes are linked to body representations and practices: e) Eating Problems (20, 13%); f) Body Health and beauty (8,07%); g) Diets and Sports ( 10,86%); h) Food quality (12, 10%). Results show a general picture of eating and body representations and practices. Eating with family is a very important social situation: a script characterised by social actors, rules, actions and contexts is outlined. Food intake control and food preferences seem pregnant dimensions in identity construction process face to family and peers. Naive theories about eating disorders are constructed. Finally diet, sport and food quality seem central in shaping and modelling body. [less ▲]

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See detailKöln und das 'Oberländische' im Spätmittelalter
Möller, Robert ULg

in Rheinische Vierteljahrsblätter (2001), 65

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See detailAntimicrobial susceptilities of Salmonella strains isolated from food animals.
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

in Antimicrobial Agents and Chemotherapy (2001), 48

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See detailMercury porosimetry: applicability of the buckling-intrusion mechanism to low-density xerogels
Alié, Christelle ULg; Pirard, René ULg; Pirard, Jean-Paul ULg

in Journal of Non-Crystalline Solids (2001), 292(1-3), 138-149

Mineral materials can be either crushed or invaded by mercury during mercury porosimetry experiments. It has been shown here that many low-density xerogels exhibit the two volume variation mechanisms ... [more ▼]

Mineral materials can be either crushed or invaded by mercury during mercury porosimetry experiments. It has been shown here that many low-density xerogels exhibit the two volume variation mechanisms successively, compaction followed by intrusion. when submitted to mercury porosimetry and that a unimodal pore size distribution can be obtained by applying Pirard's collapse model below the pressure of transition P-1 and Washburn's intrusion theory above P-t. To confirm the validity of the use of the buckling law, one low-density xerogel was wrapped in a tight membrane (intrusion is prevented and the sample is crushed during the whole porosimetry experiment). The analysis of the mercury porosimetry data of the wrapped sample by the buckling law leads to a continuous unimodal distribution similar to the distribution of the unwrapped sample obtained by applying the buckling law below P-t and the intrusion law above P-t. The position of P-t is characteristic of the tested material: when submitted to mercury pressure. aerogels and low-density xerogels only collapse in case of very small aggregates whereas they are crushed and then intruded in case of larger silica aggregates. The fact that compacted slabs of monodisperse non-aggregated silica spheres (of the same size range as the xerogels and aerogels) show only intrusion during mercury porosimetry experiments implies that the particles need to be aggregated so that the compaction mechanism takes place. The position of the change of mechanism from crushing to intrusion is not directly related to the size of the elementary particles but is linked to the size of the aggregates of silica particles. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailLes nouveaux critères de sélection porcine
Leroy, Pascal ULg

in Carrefour des Productions animales Gembloux (2001)

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See detailCessation of intensive treatment with recombinant human erythropoietin is followed by secondary anemia.
Piron, Maude ULg; Loo, Martine; Gothot, André ULg et al

in Blood (2001), 97(2), 442-8

Little information is available on the evolution of erythropoiesis after interruption of recombinant human erythropoietin (rHuEpo) therapy. Iron-overloaded rats received 20 daily injections of rHuEpo ... [more ▼]

Little information is available on the evolution of erythropoiesis after interruption of recombinant human erythropoietin (rHuEpo) therapy. Iron-overloaded rats received 20 daily injections of rHuEpo. During treatment, reticulocytes, soluble transferrin receptor (sTfR), and hematocrit increased progressively. This was accompanied by a substantial expansion of spleen erythropoiesis but a decrease in the bone marrow. Five weeks after treatment, rats developed a significant degree of a regenerative anemia. Erythropoietic activity, as assessed by reticulocytes, sTfR, erythroid cellularity, iron incorporation into heme, and the number of erythroid colonies, was severely depressed 3 weeks after cessation of rHuEpo. This was followed by regeneration of erythroblasts and reticulocytes at weeks 6 to 7 post-Epo, but erythroid progenitors recovered only partially by that time. The anemia was definitely corrected 2 months after cessation of rHuEpo treatment. Serum Epo levels remained elevated for several weeks, but the sensitivity of marrow erythroid precursors to Epo was preserved. No rat antibodies to rHuEpo were detected, and serum from post-Epo animals did not exert any inhibitory activity on erythropoiesis. In conclusion, after cessation of intensive rHuEpo therapy, there was a strong inhibition of erythropoietic activity with secondary anemia followed by late recovery. This was not due to antibodies or other soluble inhibitory factors, a defect in endogenous Epo production, or a loss of sensitivity to Epo. This may rather represent intrinsic erythroid marrow exhaustion, mostly at the level of erythroid progenitors but also at later stages of erythropoiesis. [less ▲]

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See detailDynamics of copepod faecal pellets in relation to a Phaeocystis dominated phytoplankton bloom: characteristics, production and flux
Frangoulis, Costa; Belkhiria, Sami; Goffart, Anne ULg et al

in Journal of Plankton Research (2001), 23(1), 75-88

Copepod faecal pellet characteristics and production were measured in spring 1995, 1996 and 1997 in the North Sea Southern Bight in order to define changes due to the development of the phytoplankton ... [more ▼]

Copepod faecal pellet characteristics and production were measured in spring 1995, 1996 and 1997 in the North Sea Southern Bight in order to define changes due to the development of the phytoplankton bloom. Changes were related to the succession from diatoms to a Phaeocystis sp. bloom. Mean monthly pellet production decreased during the Phaeocystis bloom development to 0.27 pellets ind. –1 h –1, approximately 80% lower than before and after the bloom. Although phytoplanktonic pigments showed significant differences between inshore and offshore stations, there was no such significant difference for faecal pellet production. Faecal pellet sinking-rate decreased from 100 to 70 m day –1 during the transition from a diatom- to a Phaeocystis-dominated bloom. This was due to a decrease in pellet density and/or a decrease of the pellet volume. These results supported the idea of lower feeding of copepods under Phaeocystis bloom conditions. As mean seasonal density of copepod faecal pellets was higher (1.37 g cm –3) than in other seas, accidental ingestion of sedimentary material as the cause of this high density is discussed. [less ▲]

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See detailPreparation of low-density xerogels by incorporation of additives during synthesis
Alié, Christelle ULg; Ferauche, Fabrice; Pirard, René ULg et al

in Journal of Non-Crystalline Solids (2001), 289(1-3), 88-96

Low-density xerogels were prepared by incorporation of an additive to alcogels prior to gelation. The additives studied are 3-(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3 ... [more ▼]

Low-density xerogels were prepared by incorporation of an additive to alcogels prior to gelation. The additives studied are 3-(2-aminoethylamino)propyltrimethoxysilane (EDAS), 3-aminopropyltrimethoxysilane (AMS), propyltrimethoxysilane (PMS), tetramethylorthosilicate (TMOS) and 3-aminopropyltriethoxysilane (AES) using tetraethylorthosilicate (TEOS) as main silica precursor. Samples were also prepared with EDAS as additive and TMOS as main silica reagent. When the additive contains methoxy groups, it reacts first, forms nuclei on which the main reagent TEOS reacts to form the silica particles. The nucleation mechanism by the additive occurs only in case of a difference of reactivity between additive and main silica precursor. The other group of the additive (amine, alkyl group, ...) influences only the gelation time. In case of ethoxy groups (series AES/TEOS) or methoxy groups (series EDAS/TMOS) for both additive and main reagent, there is no nucleation by the additive. (C) 2001 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailCriterii de differentiere a unor specii de Veronica
Crisan, Gianina; Tamas, M.; Garbacki, Nancy ULg et al

in Farmacia (2001), 49(6), 67-73

We have tried to establish some criteria to avoid the substitution of Veronica officinalis L; with anti-ulcer and anti-inflammatory activities with other species of the genus Veronica, especially Veronica ... [more ▼]

We have tried to establish some criteria to avoid the substitution of Veronica officinalis L; with anti-ulcer and anti-inflammatory activities with other species of the genus Veronica, especially Veronica chamaedrys L. widely spread and without therapeutic use. We have studied the different histological, anatomical and phytochemical characters for the two species. A rapid method for the identification of both species is the TLC for flavonoids and phenylpropanic compounds was described. Moreover HPLC is useful to detect the presence of acteoside only in Veronica chamaedrys, while Veronica officinalis contains isoacteoside. [less ▲]

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See detailRheinische Wortgeographie - dialektometrische Kartenauswertung
Möller, Robert ULg

in Dialectologia et Geolinguistica (2001), 9

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See detailAbsolute configuration of N-[(R)-3-methyl-2-butyl]-(S)-2-(6-methoxy-2-naphthyl)-propionamide
Dupont, L.; Khellili, S.; Pirotte, Bernard ULg et al

in Acta Crystallographica (2001), E57

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See detailTime-dependent wave-packet treatment of the Si4+ + He collision
Vaeck, Nathalie; Bacchus-Montabonel, Marie-Christine; Baloïtcha, Ezinvy et al

in Physical Review. A (2001), 63

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See detailL'etude clinique du mois. Apres la tempete sur les anorexigenes centraux, l'etude "STORM" avec la sibutramine.
Scheen, André ULg

in Revue Médicale de Liège (2001), 56(1), 56-8

The results of the "Sibutramine Trial of Obesity Reduction and Maintenance" (STORM) published in the last issue of December 2000 of the Lancet are summarized. This clinical trial (open label for the first ... [more ▼]

The results of the "Sibutramine Trial of Obesity Reduction and Maintenance" (STORM) published in the last issue of December 2000 of the Lancet are summarized. This clinical trial (open label for the first 6 months and double blind versus placebo for a further 18 months) demonstrates the positive effect of sibutramine, a new anorectic drug, on long-term weight maintenance after weight loss in obese subjects. The benefit of sibutramine on the cardiovascular risk profile of the obese patient is more particularly discussed. [less ▲]

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See detailAdaptations hormono-metaboliques a l'exercice musculaire.
Scheen, André ULg; Jandrain, Bernard ULg

in Revue Médicale de Liège (2001), 56(4), 195-9

Physical exercise induces numerous metabolic and hormonal changes that may be influenced by various factors, among which the intensity and the duration of the exertion seem to play a major role besides ... [more ▼]

Physical exercise induces numerous metabolic and hormonal changes that may be influenced by various factors, among which the intensity and the duration of the exertion seem to play a major role besides the possible influence of the intake of exogenous substrates. Such modifications are necessary to satisfy the marked increase in energy demand by exercising muscles, while maintaining plasma glucose concentrations within physiological range. The purpose of the present concise review is to analyse the most important fuel-hormonal adaptations observed during short heavy muscular exercise or during prolonged exercise of moderate intensity as well as those observed after physical training in normal subjects. [less ▲]

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See detailEuropean Citizenship Practice - A. Wiener
Martiniello, Marco ULg

in Nations & Nationalism (2001), 7(1), 121-122

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See detailInfo-congres. La cochrane collaboration, porte-parole de la medecine factuelle.
Scheen, André ULg

in Revue Médicale de Liège (2001), 56(11), 796-8

During the 9th International Cochrane Colloquium (Lyon October 9-13, 2001), around 700 participants discussed about how to make the evidence dissemination process more efficient in order to promote the ... [more ▼]

During the 9th International Cochrane Colloquium (Lyon October 9-13, 2001), around 700 participants discussed about how to make the evidence dissemination process more efficient in order to promote the Evidence-Based Medicine. The best evidence is summarized in systematic reviews of randomised and/or controlled clinical trials within the Cochrane Library which are prepared with a high methodological quality. However, these reviews should be completed to cover all medical domains, be actualized regularly, and, most importantly, be better known and more largely used by physicians in routine practice. All these objectives could not be attained without an official recognition and a financial support by the public health authorities. [less ▲]

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See detailLivres de médecine illustrés dans l'Egypte gréco-romaine
Marganne, Marie-Hélène ULg; Istasse, Nathaël

in Medicina nei Secoli : Arte e Scienza (2001), 13(1), 1-23

The paper deals with the illustrated medical texts from Hellenism to Byzantine age. It refers particularly to the two herbaria of Tebnytis and Antinoopolis, reconstructing the history and production ... [more ▼]

The paper deals with the illustrated medical texts from Hellenism to Byzantine age. It refers particularly to the two herbaria of Tebnytis and Antinoopolis, reconstructing the history and production techniques of scientific illustrated books of antiquity. [less ▲]

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See detailEffects of lesions of the medial preoptic nucleus on the testosterone-induced metabolic changes in specific brain areas in male quail
Balthazart, Jacques ULg; Stamatakis, A.; Bacola, S. et al

in Neuroscience (2001), 108(3), 447-466

The effects of bilateral lesions of the medial preoptic nucleus in association with testosterone on the metabolic activity in discrete brain regions was studied quantitatively by the in vivo ... [more ▼]

The effects of bilateral lesions of the medial preoptic nucleus in association with testosterone on the metabolic activity in discrete brain regions was studied quantitatively by the in vivo autoradiographic 2-deoxyglucose method. Adult male quail were castrated and then left without hormone replacement therapy or treated with testosterone or treated with testosterone and submitted to a bilateral lesion of the medial preoptic nucleus, a brain region that plays a key role in the activation of male copulatory behavior by testosterone. Treatment for about 10 days with testosterone activated the expression of the full range of male sexual behaviors and these behaviors were completely suppressed by the medial preoptic nucleus lesions. Mapping of 2-deoxyglucose uptake revealed both increases and decreases of metabolic activity in discrete brain regions associated with the systemic treatment with testosterone as well as with the lesion of the medial preoptic nucleus. Testosterone affected the oxidative metabolism in brain areas that are known to contain sex steroid receptors (such as the nucleus taeniae and the paraventricular and ventromedial nuclei of the hypothalamus) but also in nuclei that are believed to be devoid of such receptors. Effects of testosterone in these nuclei may be indirect or reflect changes in terminals of axons originating in steroid-sensitive areas. Bilateral medial preoptic nucleus lesions affected 2-deoxyglucose uptake in a variety of brain regions. Some of these regions are known to be mono-synaptically connected to the medial preoptic nucleus. Metabolic depression in these areas may reflect retrograde changes in the neurons projecting to the damaged field. The metabolic changes identified in the present study confirm the prominent role of the preoptic area in the control of sexual behavior, show that changes in the physiology of the visual system represent one of the ways through which testosterone influences the occurrence of this behavior and demonstrate that the medial preoptic nucleus has marked effects on the metabolic activity in a variety of limbic and telencephalic structures. This study also indicates that the medial preoptic nucleus affects the activity of the area ventralis of Tsai, a dopaminergic area known to send projections to a variety of hypothalamic, thalamic and mesencephalic nuclei that are implicated in the control of male sexual behavior. These data therefore support the notion that the control of the dopaminergic activity in the area ventralis of Tsai by the medial preoptic nucleus represents one of the ways through which the medial preoptic area regulates male reproductive behavior. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailModelling short-term CO2 fluxes and long-term tree growth in temperate forests with ASPECTS
Rasse, Daniel P.; François, Louis ULg; Aubinet, Marc ULg et al

in Ecological Modelling (2001), 141(1-3), 35-52

The net ecosystem exchange (NEE) Of CO2 between temperate forests and the atmosphere governs both carbon removal from the atmosphere and forest growth. In recent years, many experiments have been ... [more ▼]

The net ecosystem exchange (NEE) Of CO2 between temperate forests and the atmosphere governs both carbon removal from the atmosphere and forest growth. In recent years, many experiments have been conducted to determine temperate forest NEE. These data have been used by forest modellers to better understand the processes that govern CO, fluxes, and estimate the evolution of these fluxes under changing environmental conditions. Nevertheless, it is not clear whether models capable of handling short-term processes, which are mostly source-driven, can provide an accurate estimate of long-term forest growth, which is potentially more influenced by sink- and phenology-related processes. To analyse the interactions between short- and long-term processes, we developed the ASPECTS model, which predicts long-term forest growth by integrating, over time, hourly NEE estimates. Validation data consisting of measurements of NEE by eddy-covariance and forest carbon reservoir estimates were obtained from mixed deciduous and evergreen experimental forests located in Belgium. ASPECTS accurately estimated both: (1) the NEE fluxes for several years of data; and (2) the amount of carbon contained in stems, branches, leaves, fine and coarse roots. Our simulations demonstrated that: (1) NEE measurements in Belgian forests are compatible with forest growth over the course of the 20th century, and (2) that forest history and long-term processes need to be considered for accurate simulation of short-term CO2 fluxes. [less ▲]

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See detailLa métallurgie ancienne en "Terre de Durbuy" : Utilisation des macroscories en dynamique fluviale
Houbrechts, Geoffrey ULg; Petit, François ULg

in Bulletin de la Société Géographique de Liège (2001), 40

Avant d’utiliser les scories métallurgiques comme traceur et indicateur de la compétence de l’Aisne à différents endroits de son cours, nous avons relevé les différents atouts qui ont favorisé le ... [more ▼]

Avant d’utiliser les scories métallurgiques comme traceur et indicateur de la compétence de l’Aisne à différents endroits de son cours, nous avons relevé les différents atouts qui ont favorisé le développement de la sidérurgie dans la « Terre de Durbuy » et nous avons étudié l’évolution des techniques sidérurgiques afin de mieux comprendre la genèse des scories. Dans cet article, nous étudions plus particulièrement l’évolution longitudinale de la taille des scories dans l’Aisne, entre Blier et Bomal où elle se jette dans l’Ourthe. Vu la trop grande proximité des sites de rejets, nous n’avons pu proposer qu’une vitesse minimum d’avancée de la charge de fond ; les scories prélevées nous ont cependant renseignés sur les variations de puissances spécifiques dans les différents tronçons de la rivière. [less ▲]

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See detailLiquid chromatographic analysis of local anesthetics in human plasma after sample preparation by on-line dialysis. Optimization by use of experimental design
Chiap, Patrice ULg; Boulanger, Bruno ULg; Fotsing, Lucas ULg et al

in Chromatographia (2001), 53(11-12), 678-686

A fully automated method involving dialysis, clean-up and enrichment of the dialysate on a pre-column packed with a strong cation-exchange phase, and subsequent liquid chromatographic (LC) analysis with ... [more ▼]

A fully automated method involving dialysis, clean-up and enrichment of the dialysate on a pre-column packed with a strong cation-exchange phase, and subsequent liquid chromatographic (LC) analysis with UV detection at 220 nm has been developed for the determination of local anesthetics (prilocaine, mepivacaine, and bupivacaine) in human plasma. All sample-handling operations were executed automatically by means of an Asted XI system. To identify the most important conditions affecting analyte recovery from the dialysis and trace-enrichment processes, a seven-factor D-optimal design with 16 experimental points was elaborated as a screening test A four-factor D-optimal design with 24 experimental points was then used to predict and optimize analyte recovery. Derringer's desirability function was also used to deduce optimum conditions for analyte recovery and dialysis time within the experimental domain. Finally, the method developed was validated. Mean recoveries were approximately 72% for bupivacaine and approximately 67% for mepivacaine and prilocaine. The limits of quantification were 28 ng mL(-1) for bupivacaine and 25 ng mL(-1) for mepivacaine and prilocaine. [less ▲]

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See detailConditioned stimulus preference after acetaldehyde but not ethanol injections
Quertemont, Etienne ULg; De Witte, Philippe

in Pharmacology, Biochemistry & Behavior (2001), 68

Acetaldehyde, the first ethanol metabolite, has been suggested to mediate some of the behavioral effects of ethanol and particularly its reinforcing properties, although this later hypothesis remains ... [more ▼]

Acetaldehyde, the first ethanol metabolite, has been suggested to mediate some of the behavioral effects of ethanol and particularly its reinforcing properties, although this later hypothesis remains extremely controversial. While several studies demonstrated the reinforcing effects of brain acetaldehyde, blood acetaldehyde accumulation is believed to be primarily aversive. In the present study, a conditioned reinforcement procedure has been used to investigate the reinforcing and/or aversive effects of intraperitoneal injections of both acetaldehyde and ethanol in Wistar rats. An olfactory stimulus was paired with daily injections of either ethanol (0, 0.25, 0.5, 1 and 2 g/kg) or acetaldehyde (0, 10, 20, 100 and 150 mg/kg). After eight conditioning sessions, all rats were tested for their stimulus preference or aversion. The results show that conditioning with small, 0.25 and 0.5 g/kg, ethanol doses induced neither preference nor aversion for the olfactory cue. In contrast, higher ethanol doses (1.0 and 2.0 g/kg) resulted in significant stimulus aversions. Acetaldehyde conditioning led to a biphasic stimulus preference, with a maximal preference around 20 mg/kg acetaldehyde. No evidence of aversive effects was found with increasing doses of acetaldehyde, even with concentrations close to the lethal limit. The present study clearly shows that systemic acetaldehyde injections induced significant stimulus preferences. This suggests that acetaldehyde may be, at least in part, responsible for the reinforcing effects of alcohol intake. [less ▲]

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See detailDistinct pathways in the over-expression of matrix metalloproteinases in human fibroblasts by relaxation of mechanical tension.
Lambert, Charles ULg; Colige, Alain ULg; Munaut, Carine ULg et al

in Matrix Biology (2001), 20(7), 397-408

The aim of the work was to analyze, on a comparative basis, the signaling pathways operating in the regulation of a panel of matrix metalloproteinases (MMP) expressed by human dermal fibroblasts submitted ... [more ▼]

The aim of the work was to analyze, on a comparative basis, the signaling pathways operating in the regulation of a panel of matrix metalloproteinases (MMP) expressed by human dermal fibroblasts submitted to mechanical stress relaxation by cytochalasin D (CD) and in a retracting collagen gel (RCG). The mRNA steady-state level of MMPs was measured by a quantitative RT-PCR procedure using a synthetic RNA as internal standard. In monolayer, most MMPs were barely detected, except MMP-2. Disruption of the actin stress fibers by CD induced a moderate increase of MMP-2 mRNA and a much larger stimulation of MMP-3, -9, -13 and -14 mRNAs. In RCG, a significant up-regulation of these MMPs was also observed although to a lower extent than in CD-treated monolayers. Among the investigated MMPs, the MMP-8 and -11 were not reproducibly detected. MMP-2 was processed to its active form both by CD and in RCG. The CD-induced up-regulation of gene expression was largely repressed by blocking protein synthesis by cycloheximide for all the MMPs, by inhibiting the tyrosine-kinases of the src family by herbimycin A for all MMPs, except MMP-2, and by inhibiting the TPA-inducible PKC isoforms by bisindoyl maleimide for all MMPs, except MMP-14. The up-regulation induced by stress relaxation in RCG was protein synthesis-dependent for MMP-2 and MMP-13, tyrosine kinases-dependent for MMP-3 and MMP-13, as previously described for MMP-1. Inhibiting TPA-inducible PKC did not affect any MMP in RCG except MMP-13, which was strongly induced. The processing of MMP-2 was tyrosine kinases-dependent but PKC-independent. Inhibitors of the ERK1,2 and p38 MAP kinases pathways diversely affected the MMPs expression. Inhibiting the Rho-kinase activity by Y-27632 was inactive. These results point to the potent regulation operated by the status of the cytoskeleton on the cell phenotype, and to distinct regulatory pathways involved in the control of different MMPs expression. [less ▲]

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See detailRevascularisation coronaire a coeur battant par pontages exclusivement arteriels
RADERMECKER, Marc ULg; GRENADE, Thierry ULg; Dresse, Denise ULg et al

in Revue Médicale de Liège (2001), 56(6), 431-2

A case of complete arterial revascularization using both mammary arteries and the left radial artery is reported. The operation was done on the beating heart with the adjunct of a Y graft owing to a ... [more ▼]

A case of complete arterial revascularization using both mammary arteries and the left radial artery is reported. The operation was done on the beating heart with the adjunct of a Y graft owing to a severely calcified ascending aorta. This observation is the occasion to review the basic principles of beating heart surgery. [less ▲]

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See detailAdministration of erythopoietin and granulocyte colony-stimulating factor in donor/recipient pairs to collect peripheral blood progenitor cells (PBPC) and red blood cell units for use in the recipient after allogeneic PBPC transplantation.
Sautois, Brieuc ULg; Baudoux, Etienne ULg; Salmon, Jean ULg et al

in Haematologica (2001), 86(11), 1209-18

BACKGROUND AND OBJECTIVES: It may be useful to reduce the exposure of transplant recipients to homologous blood. This may be achieved by procuring donor-derived red blood cell (RBC) units, collecting more ... [more ▼]

BACKGROUND AND OBJECTIVES: It may be useful to reduce the exposure of transplant recipients to homologous blood. This may be achieved by procuring donor-derived red blood cell (RBC) units, collecting more peripheral blood progenitor cells (PBPC) with a combination of granulocyte colony-stimulating factor (G-CSF) + recombinant human erythropoietin (rHuEpo) and by administering rHuEpo post-transplantation. DESIGN AND METHODS: Eight ABO-compatible donors were treated with rHuEpo and intravenous iron to collect 12 RBC units for use in their recipients. PBPC were collected after mobilization with rHuEpo and G-CSF in the same donors. The recipients received G-CSF and rHuEpo post-transplantation. A control group of 10 donor/recipient pairs received G-CSF alone for PBPC mobilization and after the transplantation. RESULTS: Eighty-six out of 91 planned RBC units were collected in the donors without significant decrease in hematocrit because of a 4-fold increase in RBC production despite functional iron deficiency. After 2 leukaphereses, the cumulative yields of NC and CFU-GM were lower in the study group while those of BFU-E, CFU-Mix and CD34+ cells were similar. However, erythroid recovery was significantly accelerated in the study group. INTERPRETATION AND CONCLUSIONS: Collection of 12 RBC units within 6 weeks is feasible with rHuEpo and intravenous iron; this strategy allows a dramatic reduction in recipient exposure to homologous blood; rHuEpo has no synergistic effect with G-CSF for mobilization of PBPC in normal donors and may even be deleterious; and rHuEpo in the recipient may enhance erythroid engraftment. [less ▲]

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See detailPharma clinics le medicament du mois. Le nebivolol (Nobiten).
Scheen, André ULg

in Revue Médicale de Liège (2001), 56(11), 788-91

Nebivolol (Nobiten, Menarini) is a new beta-blocker recommended for the management of essential hypertension, at a dose of 5 mg once daily. It is administered as a racemic mixture of equal proportions of ... [more ▼]

Nebivolol (Nobiten, Menarini) is a new beta-blocker recommended for the management of essential hypertension, at a dose of 5 mg once daily. It is administered as a racemic mixture of equal proportions of d- and l-enantiomers. As a lipophilic agent, it is metabolised in the liver and transformed in several active metabolites, essentially via the CYP 2D6, an isoform of cytochrome P450 characterized by a genetic polymorphism. Nebivolol is highly specific for beta-1 adrenergic receptors; it is devoid of intrinsic sympathomimetic or membrane stabilising activity. Interestingly enough, it appears to have nitric oxide-mediated vasodilatory effects which might explain its favourable haemodynamic profile. Several comparative trials demonstrated an antihypertensive activity that was similar or slightly superior to that observed with various other reference antihypertensive agents. Nebivolol has an additive antihypertensive effect in combination with hydrochlorothiazide. All studies reported that the drug has a good clinical and biological tolerance profile. Therefore, nebivolol may be recommended as an alternative first-line treatment option for the management of patients with mild to moderate essential hypertension. [less ▲]

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See detailExpression of the antiangiogenic factor 16K hPRL in human HCT116 colon cancer cells inhibits tumor growth in Rag1(-/-) mice
Bentzien, F.; Struman, Ingrid ULg; Martini, J. F. et al

in Cancer Research (2001), 61(19), 7356-62

The M(r) 16,000 NH(2)-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor inhibiting endothelial cell function in vitro and neovascularization in vivo. The present study was ... [more ▼]

The M(r) 16,000 NH(2)-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor inhibiting endothelial cell function in vitro and neovascularization in vivo. The present study was undertaken to test the ability of 16K hPRL to inhibit the growth of human HCT116 colon cancer cells transplanted s.c. into Rag1(-/-) mice. For this purpose, HCT116 cells were stably transfected with an expression vector encoding a peptide that included the signal peptide and first 139 amino acid residues of human prolactin (HCT116(16K)). Stable clones of HCT116(16K) cells secreted large amounts of biologically active 16K hPRL into the culture medium. Growth of HCT116(16K) cells in vitro was not different from wild-type HCT116 (HCT116(wt)) or vector-transfected HCT116 (HCT116(vector)) cells. Addition of recombinant 16K hPRL had no effect on the proliferation of HCT116(wt) cells in vitro. Tumor growth of HCT116(16K) cells implanted into Rag1(-/-) mice was inhibited 63% in four separate experiments compared with tumors formed from HCT116(wt) or HCT116(vector) cells. Inhibition of tumor growth of HCT116(16K) cells was correlated with a decrease in microvascular density by 44%. These data demonstrate that biologically active 16K hPRL can be expressed and secreted from human colon cancer cells using a gene transfer approach and that production of 16K hPRL by these cells was capable of inhibiting tumor growth and neovascularization. These findings support the potential of 16K hPRL as a therapeutic agent for the treatment of colorectal cancer. [less ▲]

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See detailLe Finapres, une technique performante pour evaluer l'hypotension orthostatique, la neuropathie autonome et la syncope vaso-vagale.
Scheen, André ULg; Geronooz, I.; Marchand, Monique ULg

in Revue Médicale de Liège (2001), 56(8), 577-82

Finapres is a device able to continuously and non invasively measure arterial blood pressure by photoplethysmography in the finger. It can be used in various dynamic tests which involve cardiocirculatory ... [more ▼]

Finapres is a device able to continuously and non invasively measure arterial blood pressure by photoplethysmography in the finger. It can be used in various dynamic tests which involve cardiocirculatory adjustments, as a passive posture test ("tilt test") or an active orthostatic test (from squatting to standing position in the so-called squatting test). It represents a valuable help in the diagnosis of orthostatic hypotension (of endogenous or iatrogenic origin), of autonomic neuropathy (secondary to diabetes mellitus or to a neurological disease) or of vasovagal syncope. All these conditions are characterized by a defect of arterial and venous vasoconstriction and by an insufficient reflex tachycardia because of autonomic dysfunction. [less ▲]

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See detailNouvelles approches thérapeutiques puor l'hypernéphrome métastasique
Sylvestre, Rose-Marie; Jerusalem, Guy ULg; Sautois, Brieuc ULg et al

in Médecine et Hygiène (2001), 59

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See detailPDX:PBX complexes are required for normal proliferation of pancreatic cells during development.
Dutta, S.; Gannon, M.; Peers, Bernard ULg et al

in Proceedings of the National Academy of Sciences of the United States of America (2001), 98(3), 1065-70

The homeobox factor PDX-1 is a key regulator of pancreatic morphogenesis and glucose homeostasis; targeted disruption of the PDX-1 gene leads to pancreatic agenesis in pdx-1(-/-) homozygotes. Pdx-1 ... [more ▼]

The homeobox factor PDX-1 is a key regulator of pancreatic morphogenesis and glucose homeostasis; targeted disruption of the PDX-1 gene leads to pancreatic agenesis in pdx-1(-/-) homozygotes. Pdx-1 heterozygotes develop normally, but they display glucose intolerance in adulthood. Like certain other homeobox proteins, PDX-1 contains a consensus FPWMK motif that promotes heterodimer formation with the ubiquitous homeodomain protein PBX. To evaluate the importance of PDX-1:PBX complexes in pancreatic morphogenesis and glucose homeostasis, we expressed either wild-type or PBX interaction defective PDX-1 transgenes under control of the PDX-1 promoter. Both wild-type and mutant PDX-1 transgenes corrected glucose intolerance in pdx-1 heterozygotes. The wild-type PDX-1 transgene rescued the development of all pancreatic lineages in pdx-1(-/-) animals, and these mice survived to adulthood. In contrast, pancreata from pdx-1(-/-) mice expressing the mutant PDX-1 transgene were hypoplastic, and these mice died within 3 weeks of birth from pancreatic insufficiency. All pancreatic cell types were observed in pdx-1(-/-) mice expressing the mutant PDX-1 transgene; but the islets were smaller, and increased numbers of islet hormone-positive cells were noted within the ductal epithelium. These results indicate that PDX-1:PBX complexes are dispensable for glucose homeostasis and for differentiation of stem cells into ductal, endocrine, and acinar lineages; but they are essential for expansion of these populations during development. [less ▲]

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See detailEffects of Brassicaceae secondary metabolites on the Glutathione S-transferase activity from Episyrphus balteatus De Geer (Diptera: Syrphidae)
Vanhaelen, N.; Haubruge, Eric ULg; Francis, Frédéric ULg

in Pesticide Biochemistry & Physiology (2001), 71(3), 170-177

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See detailCurrent issues : varicella
Stouvenakers, Nadine ULg; Sadzot-Delvaux, Catherine ULg; Rentier, Bernard ULg

in Vaccines : Children and Practice (2001), 4

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See detailMother-Young Relationships in Belgian Blue Cattle after a Caesarean Section: Characterisation and Effects of Parity
Vandenheede, Marc ULg; Nicks, Baudouin ULg; Desiron, A. et al

in Applied Animal Behaviour Science (2001), 72(3), 281-292

The systematic use of Caesarean section in "double muscled" Belgian Blue cattle can induce ethical concerns. The aims of the following study was to characterise mother-young relationships in such a ... [more ▼]

The systematic use of Caesarean section in "double muscled" Belgian Blue cattle can induce ethical concerns. The aims of the following study was to characterise mother-young relationships in such a situation and to assess the effect of parity. Fifteen heifers and 15 cows of the Belgian Blue breed were observed using video recording when isolated with their calf during the 3 days following Caesarean, a rapid surgery with rare occurrence of aggressive behaviour and no sign of cows' discomfort or weakness. All calves were bottle-fed mother's colostrum once before first suckling occurred. Heifers' calves received a supplementary number of three such artificial meals, while cows' calves needed only one. The overall median time to first licking of the calf by the mother was 3.3min without any effect of parity. The mean licking frequency was 29.2+/-15.8 per 24h: heifers licked their calf less frequently than cows, respectively 23.4+/-15.3 per 24h versus 35.1+/-14.5 per 24h (P<0.05). The mean total licking duration was 42.2+/-25.9min per 24h: there was no significant difference between heifers and cows. The overall median time to first suckling was 6.1h, without any effect of parity. The mean suckling frequency was 8.4+/-4.8 per 24h: for half of these suckling bouts, the calf was situated on the left side of the mother, i.e. the side of the scar. The suckling frequency was negatively correlated with the number of artificial meals (r(s)=-0.45,P<0.05). Heifers suckled their calf less often than cows, respectively 6.1+/-3.1 per 24h versus 10.7+/-5.2 per 24h (P<0.01), but the left side proportion was not significantly different. The mean suckling total duration was 35.3+/-21.0min per 24h. The calf was on the left side of the mother during half of this time. Heifers suckled their calf during a shorter time than cows, respectively 26.9+/-20.0min per 24h versus 43.8+/-19.1min per 24h (P<0.05), and the left side proportion was not significantly different. Licking frequency and suckling duration were positively correlated (r=0.43,P<0.05). Only 10% of the mothers (one heifer and two cows) butted their calf and kicking was never observed. Despite the difficult comparison with results of the literature according to various methodologies, mother-young relationships were considered as similar to those reported after natural calving. In our study, cows can be considered as better mothers than heifers. [less ▲]

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See detailComment j'explore ... les differentes formes de diabete sucre: diagnostic differentiel clinique et biologique.
Scheen, André ULg; Philips, Jean-Christophe ULg; Luyckx, Françoise ULg et al

in Revue Médicale de Liège (2001), 56(9), 650-5

Owing to the increasing prevalence of diabetes mellitus and the various aspects of this disease, we present a practical approach which allows the clinician to more easily differentiate type 1 diabetes ... [more ▼]

Owing to the increasing prevalence of diabetes mellitus and the various aspects of this disease, we present a practical approach which allows the clinician to more easily differentiate type 1 diabetes, type 2 diabetes and secondary diabetes of pancreatic origin, i.e. the most common forms of diabetes mellitus. Such an approach uses simple diagnostic criteria, based upon both clinical characteristics (family history, personal history, clinical presentation) and biological markers (C-peptide, autoantibodies,...). A right diagnosis should allow to optimize the management of the diabetic patient. [less ▲]

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See detailPolymorphisms at the Five Exons of the Growth Hormone Gene in the Algarvia Goat. Possible Association with Milk Traits
Malveiro, E.; Pereirab, M.; Marques, P. X. et al

in Small animal research (2001), 41

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See detailUne innovation dans la chirurgie hellénistique: l'opération de la cataracte
Marganne, Marie-Hélène ULg

in Medizinhistorisches Journal (2001), 36(1), 23-33

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See detailApoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts.
Niland, S.; Cremer, A.; Herzhoff, K. et al

in In Vitro Cellular & Developmental Biology. Animal (2001), 37(9), 606-12

In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and ... [more ▼]

In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and immortalized by v-myc, to primary human dermal fibroblasts (NDF). Our results demonstrate that in contrast to NDF, all MSU-1.1 fibroblasts die within 3-4 d when cultured within three-dimensional contractile collagen matrices. Also, in contrast to NDF. MSU-1.1 cells die markedly in anchored collagen gels as well. Death is due to apoptosis and is attenuated by addition of antibodies against collagen-recognizing receptors alpha1beta1 and alpha2beta1. Apoptosis of NDF in collagen lattices was repressed by an inhibitor of caspase-1, which was ineffective on apoptosis of MSU-1.1. Further, apoptosis by MSU-1.l fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF. [less ▲]

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See detailIn vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne, Christophe ULg; Lapiere, C. M.; Nusgens, Betty ULg

in Cardiovascular Research (2001), 49(3), 647-58

OBJECTIVE: This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. METHODS ... [more ▼]

OBJECTIVE: This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. METHODS: The morphotype, biosynthetic phenotype and cytoskeleton organization of human umbilical vein endothelial cells (HUVEC) were analyzed on supports of variable mechanical resistance. RESULTS: Western blot analysis revealed a strong reduction of the expression of actin and focal-adhesion plaque (FAP) proteins in HUVEC organized in tube-like structures (TLS) on soft matrigel or on matrigel co-polymerized with heat-denatured collagen as compared to HUVEC remaining in a monolayer pattern on rigid matrigel-coat or on matrigel co-polymerized with type I collagen. Human skin fibroblasts morphotype was not altered in these culture conditions and the pattern of FAP proteins and actin was not modulated. By using polyacrylamide gels polymerized with various concentrations of bis-acrylamide to modulate the mechanical resistance of the support and cross-linked to a constant amount of gelatin to provide an equal density of attachment sites, it was shown that the less rigid the support, the more endothelial cells switched to a tube-like pattern. Collagen type I-induced tubulogenesis was accompanied by a profound and reversible remodeling of the actin-FAP complex suggesting a weakening of the bridging between extracellular matrix (ECM) and the cytoskeleton. Human skin fibroblasts and smooth muscle cells, used as control cells, adhered strongly to the collagen, did not form TLS and their network of actin stress fibers was not remodeled. The inhibition of collagen type I-induced tubulogenesis by agents altering the actin cytoskeleton-FAP complex including calpain type I inhibitor, orthovanadate, KT5720 and jasplakinolide, further supports the determinant role of mechanical coupling between the cells and the matrix in tubulogenesis. CONCLUSIONS: A reduced tension between the endothelial cells and the extracellular matrix, originating in the support or within the cells is sufficient to trigger an intracellular signaling cascade leading to tubulogenesis, an event mimicking one of the last steps of angiogenesis. [less ▲]

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See detailDiagnosis of vertical velocities with the QG Omega equation: a relocation method to obtain pseudo-synoptic data sets
Rixen, Michel; Beckers, Jean-Marie ULg; Allen, J. T.

in Deep-Sea Research Part I, Oceanographic Research Papers (2001), 48(6), 1347-1373

The quantification of vertical motion and vertical fluxes is essential in our ability to predict and tolerate climate change. However, diagnostic estimations might be affected by the errors arising from ... [more ▼]

The quantification of vertical motion and vertical fluxes is essential in our ability to predict and tolerate climate change. However, diagnostic estimations might be affected by the errors arising from the necessary compromise between spatio-temporal resolution and cost of hydrographic surveys. Observations of a numerical ocean model have been made in order to test the accuracy of different sampling strategies and their possible a-posteriori corrections. A simple first-order correction method, computing a pseudo-synoptic data set from a non-synoptic data set and involving a geostrophic relocation of the stations is shown to correct significantly the synopticity error in hydrographic data, derived QG vertical motion and vertical temperature fluxes. Sensitivity analyses also show that the lack of synopticity is more critical than other factors, including the sampling resolution, the level of no-motion and the analysis. (C) 2001 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailAlgebraic decay of the survival probability in chaotic helium
Schlagheck, Peter ULg; Buchleitner, A.

in Physical Review. A (2001), 63(2), 024701

We demonstrate that the classical helium atom exhibits algebraic decay of the survival probability in the autoionizing eZe configuration. Excitation towards asymptotic orbits of marginal stability is ... [more ▼]

We demonstrate that the classical helium atom exhibits algebraic decay of the survival probability in the autoionizing eZe configuration. Excitation towards asymptotic orbits of marginal stability is identified as the cause of the nonexponential decay. Since one electron travels along a bound Kepler trajectory of infinite excitation in these orbits, we argue that the classical decay law will prevail in the quantum dynamics of a wave packet launched along the eZe configuration. [less ▲]

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See detailOptimisation and Use of Tandem-in-Time Mass Spectrometry in Comparison with Immunoassay and Hrgc/Hrms for Pcdd/F Screening
Focant, Jean-François ULg; Eppe, Gauthier ULg; De Pauw, Edwin ULg

in Chemosphere (2001), 43(4-7, May-Jun), 417-24

Rapid screening of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans using quadrupole ion storage tandem-in-time mass spectrometry (QISTMS) conjointly with polyclonal antibody ... [more ▼]

Rapid screening of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans using quadrupole ion storage tandem-in-time mass spectrometry (QISTMS) conjointly with polyclonal antibody immunoassay has been considered. The optimisation of the fragmentation of the parent ion in the trap has been completed. The analysis of fly ashes from a municipal waste incinerator contaminated at different levels has then been realised. Results obtained using QISTMS, HRMS and immunoassay are compared. [less ▲]

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See detailDatabase "SIGMA Pro" information system and bibliographical management of active substances of plant protection products and biocides.
Rung, J.; Tricot, B.; Copin, A. et al

in Toegepaste Biologische Wetenschappen) (2001), 66(2b),

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See detailMetal fluxes through the Strait of Gibraltar: the influence of the Tinto and Odiel rivers (SW Spain)
Elbaz-Poulichet, F.; Morley, N. H.; Beckers, Jean-Marie ULg et al

in Marine Chemistry (2001), 73(3-4), 193-213

A large set of new data concerning dissolved metal concentrations has been acquired in the Gulf of Cadiz and in the Strait of Gibraltar from 1996 to 1999. These data, associated with models (hydrodynamic ... [more ▼]

A large set of new data concerning dissolved metal concentrations has been acquired in the Gulf of Cadiz and in the Strait of Gibraltar from 1996 to 1999. These data, associated with models (hydrodynamic, tracer advection-dispersion and mixing), have been used to assess the influence of rivers draining the South Iberian Pyrite Belt on the Gulf of Cadiz and on the Atlantic inflow in the Strait of Gibraltar. Metal concentrations in surface waters from the Gulf of Cadiz are maximal near the mouth of the Tinto/Odiel rivers with values exceeding 50 nmol/kg (Mn), 5 nmol/kg (Ni), 30 nmol/kg (Cu), 100 nmol/kg (Zn), 0.9 nmol/kg (Cd) and 45 nmol/kg (As). From the Tinto/Odiel river, a plume of contamination follows the coast in the direction of the Strait of Gibraltar. The computation of a tracer advection-dispersion model confirms that the coastal currents carry the metals discharged from the Tinto and Odiel to the Strait of Gibraltar. From temperature-salinity and metal-salinity plots, four water masses can be recognised in the Gulf of Cadiz and in the Strait of Gibraltar: North Atlantic Surface Water (NASW), North Atlantic Central Water (NACW) and metal-enriched Spanish Shelf Waters from the Gulf of Cadiz (SSW). The Mediterranean Outflow Water (MOW) is also clearly seen at depths greater than 300 m. The chemical characteristics of these various water masses have been used in a mixing model to evaluate their relative contribution to the Atlantic inflow through the Strait of Gibraltar. These contributions are seasonally variable. in June 1997, the contribution was: 80 +/- 20%, 5 +/- 5% and 15 +/- 10% for NASW, NACW and SSW, respectively. In September, the SSW contribution was apparently negligible. Finally, these relative contributions allow the evaluation of the metal fluxes in the Strait of Gibraltar. The presence of SSW in the Strait increases the metal flux to the Mediterranean Sea by a factor of 2.3 (Cu), 2.4 (Cd), 3 (Zn) and 7 (Mn). It does not modify significantly As and Ni fluxes. (C) 2001 Published by Elsevier Science B.V. [less ▲]

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See detailHST and ground-based observations of the gravitational lens system Q1009-0252 A & B
Claeskens, Jean-François ULg; Khmil, S. V.; Lee, Dong Wook et al

in Astronomy and Astrophysics (2001), 367

In this paper, we propose a critical study of direct images and spectra obtained for the doubly imaged QSO Q1009-0252 A & B (=LBQS1009-0252 A & B). First, we report new observations with the WFPC2 onboard ... [more ▼]

In this paper, we propose a critical study of direct images and spectra obtained for the doubly imaged QSO Q1009-0252 A & B (=LBQS1009-0252 A & B). First, we report new observations with the WFPC2 onboard the Hubble Space Telescope (HST), in which the lensing galaxy is very clearly detected. We combine these with existing NICMOS observations in order to characterize the nature and redshift of the lens. Our HST observations are also fitted with simple analytical lens models; estimates expected for the time delay and M/L of the lensing galaxy are derived. Secondly, new and existing ground-based observations are used in order to establish the first multicolour lightcurves of the lensed components. Although the time resolution is low, flux variability is detected for both components. Finally, ground-based spectroscopic observations published by Surdej et al. (1993) are re-processed with a new method designed to extract spectra with small angular separations. The final spectra are analysed on the basis of a simple model including macrolensing and microlensing amplifications and dust extinction. Based on observations collected at the European Southern Observatory (La Silla, Chile) and with the Hubble Space Telescope. [less ▲]

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See detailMigraine - advances in genetic research and possible link to neurophysiological abnormalities
Sandor, Peter S.; Agosti, Reto; Schoenen, Jean ULg

in Schweizer Archiv für Neurologie, Neurochirurgie und Psychiatrie = Archives Suisses de Neurologie, Neurochirurgie et de Psychiatrie (2001), 152

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See detailDu mode de définition de deux programmes de recherche en sociologie et en ethnométhodologie
Lejeune, Christophe ULg

in Carnets de Bord (2001), 2

Cet article examine la fondation de deux programmes scientifiques via l’étude de leur bibliographie, des traductions qu’ils opèrent et de leur définition de la mauvaise façon de faire science: leur ... [more ▼]

Cet article examine la fondation de deux programmes scientifiques via l’étude de leur bibliographie, des traductions qu’ils opèrent et de leur définition de la mauvaise façon de faire science: leur pathologie. Dans le « métier de sociologue », la cohérence entre l’importation bachelardienne de la rupture épistémologique et la sociologie critique permet de réaffirmer les dangers du substantialisme. L’ethnométhodologie s’offre quant à elle la sociologie comme pathologie. Elle traduit de manière singulière les règles de méthodes de Durkheim et Parsons en phénomènes ordinaires observables et descriptibles. L’analyse de l’opération concrète de fondation des principes de méthode de ces programmes de recherche sociologique offre une occasion d’envisager différemment le rapport de l’action à son contexte, de la sociologie à son objet, de la connaissance scientifique à la connaissance ordinaire. [less ▲]

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See detailRépercussions systémiques des infections parodontales: définition d'un nouvel index parodontal
Geerts, Sabine ULg; Rompen, Eric ULg

in Actualités Dentaires de l'Université de Liège (2001), 207

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See detailThe Vacuum UV Photoabsorption spectrum of Methyl Chloride (CH3Cl) and its perdeuterated Isotopomer CD3Cl. II. A vibrational Analysis.
Locht, Robert ULg; Leyh, Bernard ULg; Hoxha, A. et al

in Chemical Physics (2001), 272

The fine structure of the vacuum UV photoabsorption spectrum of CH3Cl and CD3Cl has been analyzed in the 7.5-10.5 eV photon energy range. A large number of lines have been observed, classified and ... [more ▼]

The fine structure of the vacuum UV photoabsorption spectrum of CH3Cl and CD3Cl has been analyzed in the 7.5-10.5 eV photon energy range. A large number of lines have been observed, classified and assigned to vibrational excitation accompanying a series of Rydberg transitions. The vibronic transitions involve both Jahn-Teller distortion and spin-orbit splitting. The former effect has been evaluated by ab initio calculations showing that the 2E state (in the C3v symmetry group) splits into 2A' and 2A" states in the Cs symmetry group. The 2A' state is energetically the lowest component whereas the 2A" is found to be a transition state. The Jahn-Teller stabilisation energy and the wavenumbers associated with all vibrational modes have been calculated. Experimentally, the entire fine structure could be described by using the vibrational modes, i.e. hcomega5= 104+/-7 meV, hcomega6= 77+/-7 meV and homega=162+/-3 meV respectively, as resulting from an average over all the analyzed Rydberg states. In CD3Cl the corresponding energies are hcomega5= 81+/-4 meV, hcomega6= 66+/-5 meV and hcomega= 124+/-4 meV. These values are in good agreement with those predicted by the present ab initio calculations for the ion ground state. However, the agreement is not so good for the wavenumber omega which could be assigned to the nu3 or nu4 vibrational modes. Though some arguments favour nu4 (the CH3 umbrella mode), within the error limits on the present measurements it is formally not possible to ascribe this wavenumber to one of these two vibrations. [less ▲]

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See detailFlutter Prediction from Flight Flutter Test Data
Dimitriadis, Grigorios ULg; Cooper, Jonathan E

in Journal of Aircraft (2001), 38(2), 355-367

The most common approach to flight flutter testing is to track estimated modal damping ratios of an aircraft over a number of flight conditions. These damping trends are then extrapolated to predict ... [more ▼]

The most common approach to flight flutter testing is to track estimated modal damping ratios of an aircraft over a number of flight conditions. These damping trends are then extrapolated to predict whether it is safe to move to the next test point and also to determine the utter speed. In the quest for more reliable and efficient flight flutter testing procedures, a number of alternative data analysis methods have been proposed. Five of these approaches are compared on two simulated aeroelastic models. The comparison is based on both the accuracy of prediction and the efficiency of each method. It is found that, for simple aeroelastic systems, the Nissim and Gilyard method (Nissim, E., and Gilyard, G. B., “Method for Experimental Determination of Flutter Speed by Parameter Identification,” AIAA Paper 89-1324, 1989) yields the best flutter predictions and is also the least computationally expensive approach.However, for larger systems, simpler approaches such as the damping fit and envelope function methods are found to be most reliable. [less ▲]

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See detailCommentaire
Richelle, Marc ULg

in Intellectica (2001), 33

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See detailIgE-mediated anaphylaxis after first intravenous infusion of cyclosporine.
Ebo, D. G.; Piel, Géraldine ULg; Conraads, V. et al

in Annals of Allergy, Asthma & Immunology (2001), 87(3), 243-5

BACKGROUND: Intravenous administration of cyclosporine, which contains Cremophor EL (a polyethoxylated castor oil; BASF, Berlin, Germany), has occasionally resulted in an anaphylactic reaction. An ... [more ▼]

BACKGROUND: Intravenous administration of cyclosporine, which contains Cremophor EL (a polyethoxylated castor oil; BASF, Berlin, Germany), has occasionally resulted in an anaphylactic reaction. An apparent hypersensitivity reaction (bronchospasm and decrease in blood pressure) had occurred during heart transplantation in a 59-year-old woman after intravenous infusion of cyclosporine. Subsequent oral administration of cyclosporine precipitated no reaction. OBJECTIVE: The purpose of this study was to attempt to ascertain the mechanism responsible for the anaphylactic reaction. METHODS: Hypersensitivity investigations, including total serum IgE and allergen-specific IgE quantifications, skin testing, and basophil activation tests by flow cytometric determination of CD63 upregulation were undertaken in the study patient and in two healthy control subjects who were free of medication. RESULTS: The results of intradermal testing with Cremophor EL were positive after 15 minutes in the study patient only. Both cyclosporine and Cremophor EL induced considerable activation of the basophils from our study patient, with an upregulation of CD63 expression from 1% to 39% and 55%, respectively. In contrast, the expression of CD63 on basophils from the two control subjects remained essentially unchanged. CONCLUSIONS: The negative investigative findings in the control subjects, the patient's clinical manifestations in temporal relationship to the infusion, her positive results on intradermal testing with Cremophor, the basophil activation test results, and her uneventful course after oral administration of cyclosporine strongly support the presence of IgE antibodies to Cremophor EL in our patient. [less ▲]

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See detailVerifìcarea unei structuri metalice pentru depozitare paletizata la actinuea focului
Zaharia, R.; Franssen, Jean-Marc ULg

in Zilele Academice Timisorene Editia VII - Buletinul Stiintific al Universitatii Politehnica din Timisoara (2001), 46(60), 288-298

The paper presents a fire design study case for a high-rise storage rack supporting building. Standardised ISO and natural fire models were considered for the fire action. The advanced numerical analysis ... [more ▼]

The paper presents a fire design study case for a high-rise storage rack supporting building. Standardised ISO and natural fire models were considered for the fire action. The advanced numerical analysis was carried out by means of SAFIR program, a FEM software specialised for the mechanical analysis of structures under elevated temperatures. [less ▲]

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See detailLes notions d'indices et de charges en procédure pénale
Jacobs, Ann ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2001)

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See detailPictures in cell biology. Spatial dynamics of rRNAs.
Thiry, Marc ULg; Cheutin, T.; O'Donohue, M. F. et al

in Trends in Cell Biology (2001), 11(4), 147

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See detailGrowth patterns of Belgian Blue replacement heifers and growing males in commercial farms
de Behr, Vanessa; Hornick, Jean-Luc ULg; Cabaraux, Jean-François ULg et al

in Livestock Production Science (2001), 71

A survey was carried out to propose models of growth curves in Belgian Blue cattle less than 20 months of age and raised in commercial farms in southern Belgium. Live weight data were recorded between ... [more ▼]

A survey was carried out to propose models of growth curves in Belgian Blue cattle less than 20 months of age and raised in commercial farms in southern Belgium. Live weight data were recorded between 1993 and 1999 from 1104 breeding heifers, and 924 growing males. The linear, polynomial group, power, logistic, Brody, Gompertz, von Bertalanffy and Richards models were used and compared. A step-wise linear growth model characterized by two periods of linear growth was also evaluated. This last model has been proposed as the reference model to characterize the growth curves of Belgian Blue cattle less than 20 months old. The animals were then ranked and divided in subgroups based on percentiles and quartiles in order to compare their performances. The subgroups performing better presented a greater homogeneity. The males ranked above the 90th percentile presented a growth rate comparable to that of young males selected for artificial insemination. Cattle ranked above the third quartile presented a growth rate comparable to that of licensed animals. When reproduction performances were related to the growth rate, it appeared that the better growing heifers were bred younger and heavier than lesser performing animals. [less ▲]

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See detailThiazolidinediones and liver toxicity.
Scheen, André ULg

in Diabètes & Métabolism (2001), 27(3), 305-13

Thiazolidinediones or glitazones specifically target insulin resistance. They have proven efficacy for reducing plasma glucose levels of type 2 diabetic patients treated with diet alone, sulphonylureas ... [more ▼]

Thiazolidinediones or glitazones specifically target insulin resistance. They have proven efficacy for reducing plasma glucose levels of type 2 diabetic patients treated with diet alone, sulphonylureas, metformin or insulin. In addition, they may be associated to some improvement of cardiovascular risk profile. However, troglitazone, the first compound approved by the FDA in the US, proved to be hepatotoxic and was withdrawn from the market after the report of several dozens of deaths or cases of severe hepatic failure requiring liver transplantation. It remains unclear whether or not hepatotoxicity is a class effect or is related to the unique tocopherol side chain of troglitazone. Rosiglitazone and pioglitazone, two other glitazones, appear to have similar efficacy on blood glucose control of type 2 diabetic patients as compared to troglitazone. In controlled clinical trials, the incidence of significant increases in liver enzyme levels (ALT) was similar with rosiglitazone or pioglitazone as compared to placebo, whereas troglitazone was associated with a threefold greater incidence. In contrast to the numerous case reports of acute liver failure in patients receiving troglitzone, only two cases of severe reversible liver failure have been reported in patients treated with rosiglitazone, with a causal relationship remaining uncertain. Furthermore, no single case of severe hepatotoxicity has been reported yet with pioglitazone. While regular monitoring of liver enzymes is still recommended and more long-term data are desirable, current clinical evidence supports the conclusion that rosiglitazone and pioglitazone do not share the hepatotoxic profile of troglitazone. [less ▲]

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See detailExperimental And Conformational Analyses Of Interactions Between Butenafine And Lipids
Mingeot-Leclercq, Mp.; Gallet, X.; Flore, Christelle ULg et al

in Antimicrobial Agents and Chemotherapy (2001), 45(12), 3347-54

Butenafine (N-4-tert-butylbenzyl-N-methyl-1-naphtalenemethylamine hydrochloride) is an antifungal agent of the benzylamine class that has excellent therapeutic efficacy and a remarkably long duration of ... [more ▼]

Butenafine (N-4-tert-butylbenzyl-N-methyl-1-naphtalenemethylamine hydrochloride) is an antifungal agent of the benzylamine class that has excellent therapeutic efficacy and a remarkably long duration of action when applied topically to treat various mycoses. Given the lipophilic nature of the molecule, efficacy may be related to an interaction with cell membrane phospholipids and permeabilization of the fungal cell wall. Similarly, high lipophilicity could account for the long duration of action, since fixation to lipids in cutaneous tissues might allow them to act as local depots for slow release of the drug. We have therefore used computer-assisted conformational analysis to investigate the interaction of butenafine with lipids and extended these observations with experimental studies in vitro using liposomes. Conformational analysis of mixed monolayers of phospholipids with the neutral and protonated forms of butenafine highlighted a possible interaction with both the hydrophilic and hydrophobic domains of membrane phospholipids. Studies using liposomes demonstrated that butenafine increases membrane fluidity [assessed by fluorescence polarization of 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene and 1,6-diphenylhexatriene] and membrane permeability (studied by release of calcein from liposomes). The results show, therefore, that butenafine readily interacts with lipids and is incorporated into membrane phospholipids. These findings may help explain the excellent antifungal efficacy and long duration of action of this drug when it is used as a topical antifungal agent in humans. [less ▲]

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See detailEpidémiologie moléculaire de la fièvre aphteuse
Thiry, Etienne ULg; Baranowski, Eric; Domingo, E.

in Epidémiologie et Santé Animale (2001), 39(59-67),

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See detailCaractérisation de particules d'engrais : corrélation entre les propriétés physiques et morphométriques
Miserque, Olivier; Tissot, Serge; Pirard, Eric ULg

in Récents Progrès en Génie des Procédés (2001), 15(78),

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See detailHepatotoxicity with thiazolidinediones: is it a class effect?
Scheen, André ULg

in Drug Safety : An International Journal of Medical Toxicology & Drug Experience (2001), 24(12), 873-88

Decreased insulin sensitivity plays a major role in various human diseases. particularly type 2 diabetes mellitus, and is associated with a higher risk of atherosclerosis and cardiovascular complications ... [more ▼]

Decreased insulin sensitivity plays a major role in various human diseases. particularly type 2 diabetes mellitus, and is associated with a higher risk of atherosclerosis and cardiovascular complications. Thiazolidinediones, more commonly termed glitazones, are the first drugs to specifically target muscular insulin resistance. They have proven efficacy for reducing plasma glucose levels in patients with type 2 diabetes mellitus treated with diet alone, sulphonylureas, metformin or insulin. In addition, they are associated with some improvement of the cardiovascular risk profile. However, troglitazone, the first compound approved by the Food and Drug Administration in the US, proved to be hepatotoxic and was withdrawn from the market after the report of several dozen deaths or cases of severe hepatic failure requiring liver transplantation. It remains unclear whether or not hepatotoxicity is a class effect or is related to unique properties of troglitazone. Rosiglitazone and pioglitazone, two other glitazones, appear to have similar efficacy with regard to blood glucose control in patients with type 2 diabetes mellitus as compared with troglitazone. In controlled clinical trials, the incidence of significant (> or =3 x upper limit of normal) increases in liver enzyme levels (ALT in particular) was similar with rosiglitazone or pioglitazone as compared with placebo, whereas troglitazone was associated with a 3-fold greater incidence. In contrast to the numerous case reports of acute liver failure in patients receiving troglitzone, only a few case reports of hepatotoxicity have been reported in patients treated with rosiglitazone until now, with a causal relationship remaining uncertain. Furthermore, no single case of severe hepatotoxicity has been reported yet with pioglitazone. It should be mentioned that troglitazone, unlike pioglitazone and rosiglitazone, induces the cytochrome P450 isoform 3A4, which is partly responsible for its metabolism, and may be prone to drug interactions. Importantly enough, obesity, insulin resistance and type 2 diabetes mellitus are associated with liver abnormalities, especially non-alcoholic steatohepatitis, independent of any pharmacological treatment. This association obviously complicates the selection of patients who are good candidates for a treatment with glitazones as well as the monitoring of liver tests after initiation of therapy with any thiazolidinedione compound. While regular monitoring of liver enzymes is still recommended and more long term data are desirable, current evidence from clinical trials and postmarketing experience in the US supports the conclusion that rosiglitazone and pioglitazone do not share the hepatotoxic profile of troglitazone. [less ▲]

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See detailGap Filling Strategies For Defensible Annual Sums Of Net Ecosystem Exchange
Falge, E.; Baldocchi, D.; Olson, R. et al

in Agricultural and Forest Meteorology (2001), 107(1),

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See detailSupernumerary outer hair cells arise external to the last row of sensory cells in the organ of corti.
Lefèbvre, Philippe ULg; Malgrange, Brigitte ULg; Thiry, Marc ULg et al

in Acta Oto-Laryngologica (2001), 121(2), 164-8

During the development of the mammalian inner ear, the number of hair cells produced is highly regulated and remains constant throughout life. The mechanism underlying this regulation is beginning to be ... [more ▼]

During the development of the mammalian inner ear, the number of hair cells produced is highly regulated and remains constant throughout life. The mechanism underlying this regulation is beginning to be understood although many aspects still remain obscure. When late embryonic or early postnatal rat organs of Corti were cultured, the production of supernumerary hair cells was observed. This overproduction of sensory cells could be modulated by the addition of several growth factors. In this study, we examined explants of rat organs of Corti that produced supernumerary hair cells. In the supernumerary hair cell region, up to two rows of inner hair cells and five rows of outer hair cells were observed. Morphological evaluation of these specimens revealed that less mature hair cells were located in the most external rows of these sensory cells. When a supernumerary hair cell was produced, a supporting cell (i.e. Deiters' cell) was also produced, strongly suggesting that the conversion of a Deiters' cell into a hair cell was not the mechanism that produced these extra hair cells. Based on these results, we propose that prosensory cells located at the external edge of the organ of Corti retain a capacity to form hair cells and that it is these prosensory cells that differentiate into supernumerary hair cells and Deiters' cells. [less ▲]

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See detailAnticyclonic eddies in the deep eastern Black Sea in summer-autumn 1999 (satellite and ship-borne observations
Ginzburg, Anna I.; Zatsepin, Andrey; Kostianoy, Andrey G. et al

in Earth Observation and Remote Sensing (2001), 5

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