References of "Scientific journals"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailRed blood cell substitutes: fluorocarbon emulsions and haemoglobin solutions.
Remy, Bernadette ULg; Deby, Ginette ULg; Lamy, Maurice ULg

in British Medical Bulletin (1999), 55(1), 277-98

The problems posed by transfusion of homologous blood have led to the development of substances able to replace the gas transporting properties of blood. Perfluorocarbons (PFCs) emulsions and modified ... [more ▼]

The problems posed by transfusion of homologous blood have led to the development of substances able to replace the gas transporting properties of blood. Perfluorocarbons (PFCs) emulsions and modified haemoglobin (Hb) solutions have been developed for this goal and are now tested in clinical assays. PFCs are synthetic fluorinated hydrocarbons, capable of dissolving large quantities of oxygen (O2; without binding) at high inspired concentrations of O2, and of delivering this O2 to the tissues. They are administered as emulsions containing particles with a diameter of approximately 0.2 micron, capable of entering the microcirculation. They are eliminated unchanged by the lungs within several days. Fluosol-DA 20% was the first PFC emulsion used in clinical practice. Currently, Oxygent, a second generation PFC emulsion, is being evaluated in clinical studies. The PFCs are not blood substitutes, but rather a means to ensure tissue oxygenation during extreme haemodilution. Solutions of free Hb do not have the antigenic characteristics of the blood groups, and do not require compatibility testing. They are fully saturated with O2 at ambient FiO2. The Hbs used are derived from either human or bovine sources, or via recombinant DNA technology. In order to maintain satisfactory intravascular half-life and O2 affinity, the Hb molecules are modified by adding internal crosslinks, by polymerization, and/or by encapsulation. After promising animal studies, several of these modified Hb solutions are now being studied in Phase III clinical trials. Among them, diaspirin cross-linked haemoglobin (DCLHb) has been used in cardiac and orthopaedic surgery, and for resuscitation of traffic accident victims. The initial results of multicentre trials are now being analysed. [less ▲]

Detailed reference viewed: 23 (0 ULg)
See detailCryptospores and spores from the Ordovician to the Llandovery. A review
Steemans, Philippe ULg

in Acta Universitatis Carolinae. Geologica (1999), (1/2), 271--274

Detailed reference viewed: 24 (1 ULg)
Full Text
See detailIndices d'aptitude des procédés de production
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1999), (5), 26

This note describes the main process capability indices and presents some inferential porperties. A numerical example is analysed by Minitab and SAS softwares.

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailConvergence of High Order Curl-Conforming Finite Elements
Geuzaine, Christophe ULg; Meys, B.; Dular, Patrick ULg et al

in IEEE Transactions on Magnetics (1999), 35(3), 1442--1445

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailLes aménagements forestiers en Belgique méridionale (Wallonie) : états des lieux et perspectives
Rondeux, Jacques ULg

in Revue Forestière Française (1999), LI(numéro spécial), 241-246

The forests of French-speaking Belgium cover 530,000 hectares that are evenly distributed as between public and private property and as between deciduous and coniferous populations. Since the 90's, the ... [more ▼]

The forests of French-speaking Belgium cover 530,000 hectares that are evenly distributed as between public and private property and as between deciduous and coniferous populations. Since the 90's, the emphasis of forest policy has been on permanency and multiple-use. Forest plans, particularly in public forests, are largely shaped by production, conservation and protection functions as reflected in complete revisions of the computerised compartment register and implementation of many regulatory provisions. This article reviews existing approaches and describes the main planning methods recommended according to types of stands and on whether a forest is public or private, the latter being far less regulated. [less ▲]

Detailed reference viewed: 16 (4 ULg)
Peer Reviewed
See detailMise au point de pains composites à base de mélanges de farines de sorgho/blé et analyse texturale.
Balla, A.; Blecker, Christophe ULg; Oumarou, M. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 3(2), 69-77

Detailed reference viewed: 17 (7 ULg)
Full Text
Peer Reviewed
See detailEarly development of the chondrocranium in Chrysichtys auratus (Pisces, Siluriformes, Claroteidae)
Vandewalle, Pierre ULg; Chikou, Antoine; Lalèyé, Philippe et al

in Journal of Fish Biology (1999), 55

The inception and development of the cartilaginous cephalis skeleton of Chrysichthys auratus is described from hatching to about 18 days post-hatching. At hatching, no skeletal structure is present. Not ... [more ▼]

The inception and development of the cartilaginous cephalis skeleton of Chrysichthys auratus is described from hatching to about 18 days post-hatching. At hatching, no skeletal structure is present. Not until day 3 do clearly delimited cranial primordia become apparent. As in many siluriforms, the neurocranium is platybasic from the start, the suspensorium constitutes, with Meckel’s cartilage and the hyoid bar, a single cartilaginous element, and the junction between the front and rear of the neurocranium is complete on day 4. By day 8 the quadratomandibular joint has formed and the tectum posterius has appeared. Cartilage reduction first affects the trabecular bars, then, markedly, the visceral arches. By day 18 the braincase floor has almost disappeared. [less ▲]

Detailed reference viewed: 24 (2 ULg)
Full Text
Peer Reviewed
See detailTreatment of Osteoporosis: Role of Bone-Forming Agents
Reginster, Jean-Yves ULg; Halkin, V.; Henrotin, Yves ULg et al

in Osteoporosis International (1999), 9 Suppl 2

Detailed reference viewed: 3 (1 ULg)
Peer Reviewed
See detailIntracellular visualization of BrdU-labeled plasmid DNA/cationic liposome complexes.
El Ouahabi, A.; Thiry, Marc ULg; Schiffmann, S. et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1999), 47(9), 1159-66

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA ... [more ▼]

Difficulties in specific detection of transfected DNA in cells represent an important limitation in the study of the gene transfer process. We studied the cellular entry and fate of a plasmid DNA complexed with a cationic lipid, Vectamidine (3-tetradecylamino-N-tert-butyl-N'-tetradecylpropionamidine) in BHK21 cells. To facilitate its detection inside the cells, bromodeoxyuridine (BrdU) was incorporated into plasmid DNA under conditions that minimize plasmid alteration. BrdU was localized in cells incubated with Vectamidine/BrdU-labeled plasmid DNA complexes by immunogold labeling and electron microscopy (EM). Labeling was predominantly associated with aggregated liposome structures at the surface of and inside the cells. EM observations of cells transfected with Vectamidine/DNA complexes showed that the liposome/DNA aggregates accumulate in large vesicles in the cell cytosol. On the other hand, using rhodamine-labeled Vectamidine and revealing BrdU with FITC-conjugated antibodies permitted simultaneous detection in the cells of both components of the complexes with confocal laser scanning microscopy. The DNA and lipids co-localized at the surface of and inside the cells, indicating that the complex is internalized as a whole. Our results show that the BrdU-labeled plasmid DNA detection system can be a useful tool to visualize exogenous DNA entry into cells by a combination of electron and confocal microscopy. [less ▲]

Detailed reference viewed: 25 (6 ULg)
Full Text
Peer Reviewed
See detailScintigraphy of the equine lung
Votion, Dominique ULg; Lekeux, Pierre ULg

in Equine Veterinary Education (1999), 11

Detailed reference viewed: 6 (2 ULg)
Peer Reviewed
See detailCourteous behaviour concerning beetles sexuality
Haubruge, Eric ULg

in New Scientist (1971) (1999), 227(2184), 60

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailThe Crystal Structure Of A Penicilloyl-Serine Transferase Of Intermediate Penicillin Sensitivity - The Dd-Transpeptidase Of Streptomyces K15
Fonze, E.; Vermeire, M.; Nguyen-Disteche, M. et al

in Journal of Biological Chemistry (1999), 274(31), 21853-60

The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide bond formation in a way that mimics the penicillin-sensitive peptide cross-linking reaction involved in ... [more ▼]

The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide bond formation in a way that mimics the penicillin-sensitive peptide cross-linking reaction involved in bacterial cell wall peptidoglycan assembly. The Streptomyces K15 enzyme is peculiar in that it can be considered as an intermediate between classical penicillin-binding proteins, for which benzylpenicillin is a very efficient inactivator, and the resistant penicillin-binding proteins that have a low penicillin affinity. With its moderate penicillin sensitivity, the Streptomyces K15 DD-transpeptidase would be helpful in the understanding of the structure-activity relationship of this penicillin-recognizing protein superfamily. The structure of the Streptomyces K15 enzyme has been determined by x-ray crystallography at 2.0-A resolution and refined to an R-factor of 18.6%. The fold adopted by this 262-amino acid polypeptide generates a two-domain structure that is close to those of class A beta-lactamases. However, the Streptomyces K15 enzyme has two particular structural features. It lacks the amino-terminal alpha-helix found in the other penicilloyl-serine transferases, and it exhibits, at its surface, an additional four-stranded beta-sheet. These two characteristics might serve to anchor the enzyme in the plasma membrane. The overall topology of the catalytic pocket of the Streptomyces K15 enzyme is also comparable to that of the class A beta-lactamases, except that the Omega-loop, which bears the essential catalytic Glu(166) residue in the class A beta-lactamases, is entirely modified. This loop adopts a conformation similar to those found in the Streptomyces R61 DD-carboxypeptidase and class C beta-lactamases, with no equivalent acidic residue. [less ▲]

Detailed reference viewed: 6 (1 ULg)
Full Text
See detailMorphotypes et postures de vie et de mort chez les trilobites.
Pierard, Sébastien ULg; Franchimont, Claudine ULg; Pierard, Gérald ULg

in Revue Verviétoise d'Histoire Naturelle (1999), 56

Detailed reference viewed: 28 (1 ULg)
Peer Reviewed
See detailEffects of Inhalation of Dust and Endotoxin on Respiratory Tracts of Pigs
Urbain, B.; Mast, J.; Beerens, Dominique ULg et al

in American Journal of Veterinary Research (1999), 60(9), 1055-1060

OBJECTIVE: To assess the effects of inhalation of feed flour dust and dustborne endotoxin on respiratory tracts of pigs. ANIMALS: 29 healthy Belgian Landrace pigs. PROCEDURE: Pigs housed in an ... [more ▼]

OBJECTIVE: To assess the effects of inhalation of feed flour dust and dustborne endotoxin on respiratory tracts of pigs. ANIMALS: 29 healthy Belgian Landrace pigs. PROCEDURE: Pigs housed in an environmental chamber were exposed for 6 days to feed flour dust (1 to 15 mg/m3) and dustborne endotoxins (50 to 2,500 ng/m3). Effects were evaluated by measuring albumin concentration, lactate dehydrogenase (LDH) activity, cell composition of nasal lavage (NL) and bronchoalveolar lavage (BAL) fluids and blood, and percentages of CD4+ and CD8+ T lymphocytes in blood and lavage fluids. Dustborne endotoxin was obtained by mixing endotoxins from Escherichia coli (serotype O127:B8) with feed flour before spraying the flour in the environmental chamber. RESULTS: Exposure did not affect cell composition of NL fluid or blood. Total cell counts of BAL fluids were increased in all groups exposed to dust. Macrophage counts were increased in pigs exposed to inhalable dust concentrations as low as 4.4 mg/m3, and lymphocyte counts were increased in groups exposed to high dust concentrations. Percentages of CD4+ and CD8+ T lymphocytes in blood and lavage fluids were unchanged. In all dust-exposed groups, albumin content of BAL fluid was increased, whereas LDH activity was unaffected. Macrophage and lymphocyte infiltration and edema in the bronchi were identified by light microscopy. Effects attributable to E. coli endotoxin exposure were not identified. CONCLUSIONS: Inhalation of feed flour dust did not affect nasal mucosa but did induce bronchial airway inflammation. Dustborne endotoxins did not have effects attributable to endotoxin alone. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detail“La Destruction fut ma Béatrice”. Mallarmé ou l’implosion poétique
Durand, Pascal ULg

in Revue d'Histoire Littéraire de la France (1999), 3

Detailed reference viewed: 233 (9 ULg)
Full Text
Peer Reviewed
See detailEstimation of the charge carrier localization length from Gaussian fluctuations in the magneto-thermopower of La0.6Y0.1Ca0.3MnO3
Sergeenkov, S.; Bougrine, Hassan ULg; Ausloos, Marcel ULg et al

in Physical Review. B : Condensed Matter (1999), 60(17), 12322-12328

The magneto-thermoelectric power (TEP) Delta S(T,H) of perovskite-type manganese oxide La0.6Y0.1Ca0.3MnO3 is found to exhibit a sharp peak at some temperature T*=170 K. By approximating the true shape of ... [more ▼]

The magneto-thermoelectric power (TEP) Delta S(T,H) of perovskite-type manganese oxide La0.6Y0.1Ca0.3MnO3 is found to exhibit a sharp peak at some temperature T*=170 K. By approximating the true shape of the measured magneto-TEP in the vicinity of T* by a linear triangle of the form Delta S(T,H) similar or equal to S-p(H)+/-B+/-(H)(T* - T), we observe that B-(H)similar or equal to 2B(+)(H). We adopt the electron localization scenario and introduce a Ginzburg-Landau (GL)-type theory which incorporates the two concurrent phase transitions, viz., the paramagnetic-ferromagnetic transition at the Curie point T-C and the "metal-insulator'' (M-I) transition at T-MI. The latter is characterized by the divergence of the field-dependent charge carrier localization length xi(T,H) at some characteristic field H-o. Calculating the average and fluctuation contributions to the total magnetization and the transport entropy related magneto-TEP Delta S(T,H) within the GL theory, we obtain a simple relationship between T* and the above two critical temperatures (T-C and T-MI). The observed slope ratio B-(H)/B+(H) is found to be governed by the competition between the electron-spin exchange JS and the induced magnetic energy MsHo. The comparison of our data with the model predictions produce T-C = 195 K, JS = 40 meV, M-o = 0.4M(s), and xi(o) = 5 Angstrom for the estimates of the Curie temperature, the exchange coupling constant, the critical magnetization, and the localization length, respectively. The magneto-TEP data obtained by other authors are discussed and Found to be consistent with the model predictions as well. [[S0163-1829(99)00734-1]. [less ▲]

Detailed reference viewed: 22 (1 ULg)
Full Text
Peer Reviewed
See detailQuantum mechanical statistical theories for chemical reactivity. Overlapping resonances
Desouter, Michèle ULg; Chapuisat, Xavier

in Physical Chemistry Chemical Physics [=PCCP] (1999), 1

Full Text
Peer Reviewed
See detailL'anorexie mentale, une maladie grave specifique de la femme jeune.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(4), 255-61

Anorexia nervosa is characterized by a triad comprising anorexia, weight loss, and amenorrhoea. It is typically present in adolescent or young adult women and its prevalence appears to increase in our ... [more ▼]

Anorexia nervosa is characterized by a triad comprising anorexia, weight loss, and amenorrhoea. It is typically present in adolescent or young adult women and its prevalence appears to increase in our society. Eating disorders may lead to cachexia and various severe complications. This disease, which appears to be associated to a particular psychological background, is triggered by conflictual relationships, usually within the family. The anorectic patient should be carefully evaluated, as soon as possible, from a somatic, dietetic and psychological point of view. A specific therapeutic approach should be rapidly proposed in a specialized centre, if possible by a multidisciplinary team. The treatment is most often difficult and of long duration. Prognosis remains uncertain in most cases, especially when appropriate care is delayed. [less ▲]

Detailed reference viewed: 345 (8 ULg)
Full Text
See detailEtude des hypothèses de l'analyse de la variance à deux critères de classification : approche par l'exemple
Iemma, A. F.; Claustriaux, Jean-Jacques ULg

in Notes de Statistique et d'Informatique (1999), (3), 36

By examples and results from SAS and Minitab softwares (GLM), analysis of variance hypothesis built to analyse a two dimensional data table are introduced.

Detailed reference viewed: 26 (1 ULg)
Peer Reviewed
See detailIntroduction
Martiniello, Marco ULg; Statham, Paul

in Journal of Ethnic & Migration Studies (1999), 25(4), 565-573

Detailed reference viewed: 5 (0 ULg)
Peer Reviewed
See detailComparison of performance, water intake and feeding behaviour of weaned pigs fed either pellets or meal
Laitat, Martine ULg; Vandenheede, Marc ULg; Desiron, Alain et al

in Animal Science (1999), 69

Performance, water intake and feeding behaviour of two groups of 30 (trial 1), 40 (trial 2) or 50 (trial 3) weaned pigs offered either pellets ol meal of the same formulation were compared. Average daily ... [more ▼]

Performance, water intake and feeding behaviour of two groups of 30 (trial 1), 40 (trial 2) or 50 (trial 3) weaned pigs offered either pellets ol meal of the same formulation were compared. Average daily weight gains (ADG) were higher for pigs given pellets rather than meal in trials 2 (413 v. 363 g/day P < 0.001) and 3 (356 v. 324 g/day, P < 0.05). Mean daily water intake (DWI) was higher with meal than with pellets but only during trial 1 (2.31 y. 1.65 l/day, P < 0.01). The occupation time (OT) and the number of animals using the feeder simultaneously (N) were higher when pigs were given meal rather than pellets, whatever the animal density: trial 1: 82.6 v. 69.9% (P = 0.05) and 3.8 v. 2.3 (P < 0.01); trial 2: 90.9 v. 77.9% (P > 0.05) and 5.2 v. 3.1 (P < 0.01); trial 3: 96.2 v. 83.6% (P < 0.05) and 5.9 v. 3.8 (P < 0.01). When using pellets, OT and N were always significantly lower during the night than during the day but when using meal in groups of 40 and 50, OT during the night was almost as high as during the day. The greater the group size, the lower were ADG (both diets) and DWI (only with meal) and the higher were OT and N (both diets). Furthermore, significant linens and curvilinear regressions of DWI, OT and N according to time were calculated. In conclusion, pigs need more time to eat meal than to eat pellets. Thus the number of pigs per feeder has to be adapted to the food presentation. Too high number of pigs per feeder impairs feeding behaviour and eventually welfare, by preventing preferential diurnal feeding activity and this may affect productivity [less ▲]

Detailed reference viewed: 86 (0 ULg)
Full Text
Peer Reviewed
See detailCharacterization Of Functional Residues In The Interfacial Recognition Domain Of Lecithin Cholesterol Acyltransferase (Lcat)
Peelman, F.; Vanloo, B.; Perez-Mendez, O. et al

in Protein Engineering (1999), 12(1), 71-8

Lecithin cholesterol acyltransferase (LCAT) is an interfacial enzyme active on both high-density (HDL) and low-density lipoproteins (LDL). Threading alignments of LCAT with lipases suggest that residues ... [more ▼]

Lecithin cholesterol acyltransferase (LCAT) is an interfacial enzyme active on both high-density (HDL) and low-density lipoproteins (LDL). Threading alignments of LCAT with lipases suggest that residues 50-74 form an interfacial recognition site and this hypothesis was tested by site-directed mutagenesis. The (delta56-68) deletion mutant had no activity on any substrate. Substitution of W61 with F, Y, L or G suggested that an aromatic residue is required for full enzymatic activity. The activity of the W61F and W61Y mutants was retained on HDL but decreased on LDL, possibly owing to impaired accessibility to the LDL lipid substrate. The decreased activity of the single R52A and K53A mutants on HDL and LDL and the severer effect of the double mutation suggested that these conserved residues contribute to the folding of the LCAT lid. The membrane-destabilizing properties of the LCAT 56-68 helical segment were demonstrated using the corresponding synthetic peptide. An M65N-N66M substitution decreased both the fusogenic properties of the peptide and the activity of the mutant enzyme on all substrates. These results suggest that the putative interfacial recognition domain of LCAT plays an important role in regulating the interaction of the enzyme with its organized lipoprotein substrates. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detail3-Alkylamino-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxides as potent KATP-channel activators: structural study and influence of stereochemistry
De Tullio, Pascal ULg; Khelili, S.; Ouedraogo, R. et al

in Pharmacy and Pharmacology Communications (1999), 5

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailCytokinin modulates catalase activity and coumarin accumulation in in vitro cultures of tobacco
Petit-Paly, Geneviève; Franck, Thierry ULg; Brisson, Louise et al

in Journal of Plant Physiology (1999), 155(1), 9-15

Cytokinins (CKs) represent an important class of phytohormones particularly known for their antisenescence properties that might be regulated through an effect on the oxidative metabolism. In the present ... [more ▼]

Cytokinins (CKs) represent an important class of phytohormones particularly known for their antisenescence properties that might be regulated through an effect on the oxidative metabolism. In the present work, we demonstrate the effect of CKs on catalase activity in tobacco cultivated in vitro. The catalase activity observed in suspension-cultured cells decreased slightly during the first hour of CK treatment and increased thereafter to double the level detected in untreated cells. In contrast to these results, catalase activity was inhibited in shoot cultures in which the endogenous levels of CK were elevated by the introduction of the isopentenyltransferase gene or by an exogenous feeding of CK to the cultures. Interestingly, this catalase inhibition correlated with an accumulation of scopolin, an inducible coumarin. Taken together, our results show that CK modulates (directly or undirectly) catalase activity. The inverse relationship that was always found between scopolin accumulation and catalase activity is discussed in terms of vitrification and habituation. [less ▲]

Detailed reference viewed: 66 (1 ULg)
Peer Reviewed
See detailUn réformateur lotharingien de choc : l'abbé Poppon de Stavelot (978-1048)
George, Philippe ULg

in Revue Mabillon (1999)

Detailed reference viewed: 27 (0 ULg)
Full Text
Peer Reviewed
See detailLa lecture par la Cour d'arbitrage des condamnations d'office à caractère forfaitaire
Michiels, Olivier ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (1999), 37

L'article s'attache à analyser le caractère juridique des condamnations d'office visées par l'article 35 de la loi du 27 juin 1969 sur la sécurité sociale des travailleurs salariés.

Detailed reference viewed: 26 (4 ULg)
Full Text
Peer Reviewed
See detailPreparation and pharmacological evaluation of the R- and S-enantiomers of 3-(2’-butylamino)-4H- and 3-(3’-methyl-2’-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide, two tissue selective ATP-sensitive potassium channel openers
Khelili, S.; De Tullio, Pascal ULg; Lebrun, P. et al

in Bioorganic & Medicinal Chemistry (1999), 7(8), 1513-1520

The preparation and the pharmacological evaluation of the R- and S-isomers of 3-(2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 42) and 3-(3-methyl-2-butylamino)-4H-pyrido[4,3-e]-1,2,4 ... [more ▼]

The preparation and the pharmacological evaluation of the R- and S-isomers of 3-(2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 42) and 3-(3-methyl-2-butylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide (BPDZ 44), two potassium channel openers, is described. Their optical purity was estimated by means of capillary electrophoresis (R- and S-BPDZ 42) and chiral HPLC (R- and S-BPDZ 44). The absolute configuration of each isomer of BPDZ 44 was deduced from crystallographic data. Pharmacological assays performed with the R- and S-isomers of BPDZ 44 revealed only slight differences in their activity on pancreatic B-cells but significant differences in their activity on vascular smooth muscle cells: the R-isomer being sixfold more potent than its corresponding S-isomer. The R-isomer of BPDZ 42 was shown to be more potent than its corresponding S-isomer on the endocrine pancreas. S-BPDZ 44 as well as R- and S-BPDZ 42 were found to exhibit tissue selectivity for the pancreatic versus the vascular smooth muscle tissue. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailCerebral metabolism during vegetative state and after recovery to consciousness
Laureys, Steven ULg; Lemaire, Christian ULg; Maquet, Pierre ULg et al

in Journal of Neurology, Neurosurgery & Psychiatry (1999), 67(1), 121-122

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detail"CAPCIL". Adaptation posologique des traitements par aminoglycosides
Gougnard, Thierry; Charlier, Corinne ULg; Plomteux, Guy ULg

in Acta Clinica Belgica. Supplementum (1999), 1

The objectives of the therapeutic drug monitoring are to assume the efficacy and inocuity of a medical treatment and the patient's observance. The administration of a drug to a patient is not always ... [more ▼]

The objectives of the therapeutic drug monitoring are to assume the efficacy and inocuity of a medical treatment and the patient's observance. The administration of a drug to a patient is not always performed in the same conditions and therefore treatment has to be adapted. When necessary, this one is very often based on empiric or very approximative notions and, more seldom, on results of plasmatic concentrations of the drug. The CAPCIL program allows the possibility to objectivate the medical decision and adapt the posology on the basis of two kinetic parameters: the biological half-life and the distribution volume. Indeed, most of pharmacokinetics modifications (drug interactions, diseases, ...) are affecting the two parameters. With basic informations so as height and weight, posology, treatment objectives and peak/trough plasmatic concentrations of the drug, the program is proposing several posology adaptation schemes. The example of a once-a-day administration of amikacin is discussed. [less ▲]

Detailed reference viewed: 36 (0 ULg)
Full Text
Peer Reviewed
See detailAnesthesie generale versus anesthesie perimedullaire.
Murayi, P.; Joris, Jean ULg; Lamy, Maurice ULg

in Revue Médicale de Liège (1999), 54(7), 588-92

Perimedullary anesthetic techniques (epidural or spinal anesthesia), by themselves or combined with general anesthesia offer several advantages in the postoperative period when compared with general ... [more ▼]

Perimedullary anesthetic techniques (epidural or spinal anesthesia), by themselves or combined with general anesthesia offer several advantages in the postoperative period when compared with general anesthesia alone. The incidence of postoperative respiratory and cardiovascular complications is decreased. The physiologic stress reaction, with its associated hypercoagulable state and immune depression, is attenuated. Finally, the resumption of gastrointestinal function is hastened. These benefits of central neural blockade are noted most clearly when the techniques are used for several days postoperatively, most often by catheter based epidural analgesia. The use of local anesthetic agents in the analgesic mixture would appear to be important. This is likely because these substances inhibit the sympathetic nervous system and spinal reflex axes. The role of this inhibition in the advantages of perimedullary techniques is probably important. It is important to note that inhibition of the sympathatetic nervous system can be associated with indesirable consequences in certain patients. These techniques must therefore be used cautiously, and patients who benefit from them must receive careful surveillance. [less ▲]

Detailed reference viewed: 74 (3 ULg)
See detailL’acromégalie et son impact sur la fonction reproductrice
Beckers, Albert ULg; Stevenaert, Achille ULg

in Glunaïkeia (1999), 4(8), 212-218

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailClinical Activity and Benefit of Irinotecan (CPT-11) in Patients with Colorectal Cancer Truly Resistant to 5-Fluorouracil (5-FU)
Van Cutsem, Eric; Cunningham, D.; Ten Bokkel Huinink, W. W. et al

in European Journal of Cancer (1999), 35

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailTwo new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide.
Fanuel, L; Thamm, Iris ULg; Kostanjevecki, V et al

in Cellular and Molecular Life Sciences : CMLS (1999), 55(5), 812-8

Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the ... [more ▼]

Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the Dap protein produced by O. anthropi SCRC C1-38 (ATCC49237). The second enzyme, DmpA, exhibits a similar substrate profile when tested on p-nitroanilide derivatives of glycine and L/D-alanine, but the amounts produced by the Ochrobactrum strain were not sufficient to allow complete purification. Interestingly, the DmpA preparation also exhibited an L-aminopeptidase activity on the tripeptide L-Ala-Gly-Gly but it was not possible to be certain that the same protein was responsible for both p-nitroanilide and peptide hydrolysing activities. The gene encoding the DmpA protein was cloned and sequenced. The deduced protein sequence exhibits varying degrees of similarity with those corresponding to several open reading frames found in the genomes of other prokaryotic organisms, including Mycobacteria. None of these gene products has been isolated or characterised, but a tentative relationship can be proposed with the NylC amidase from Flavobacterium sp. K172. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailUltrastructural methods for nucleic acid detection by immunocytology.
Thiry, Marc ULg

in Progress in Histochemistry and Cytochemistry (1999), 34(2), 87-159

In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to ... [more ▼]

In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to natural nucleic acids are described but also the techniques involving the use of antibodies raised against various nucleotide analogs incorporated beforehand into nucleic acids. Special emphasis is placed on each method's potential and limitations. These methods, combined or not with molecular biotechnology, are powerful tools for studying the structure and function of nucleic acids. They can be used to investigate the distribution and topological organization of DNA and RNA molecules or of specialized within these molecules in the cells. [less ▲]

Detailed reference viewed: 7 (2 ULg)
Peer Reviewed
See detailAgriculture et utilisation raisonnée de pesticides
Francis, Frédéric ULg; Gaspar, C.

in Canard Déchaîné du Kauwberg (Le) (1999), 32

Detailed reference viewed: 46 (6 ULg)
Full Text
Peer Reviewed
See detailStructure of high-temperature fluid selenium
Raty, Jean-Yves ULg; Saul, A.; Gaspard, Jean-Pierre ULg et al

in Physical Review. B : Condensed Matter (1999), 60(4), 2441-24482448

A semiempirical tight-binding energy model is developed for selenium. It includes s and p electrons as well as an empirical description of the dispersion forces that proves necessary at the liquid ... [more ▼]

A semiempirical tight-binding energy model is developed for selenium. It includes s and p electrons as well as an empirical description of the dispersion forces that proves necessary at the liquid densities under study. The band-structure parameters are obtained by fitting abinitio calculations. The simulated liquid structures are in very good agreement with the most recent X-ray scattering and extended X-ray absorption fine-structure experiments in a broad temperature and density range. The Monte Carlo simulations performed show that the complex liquid structures observed result from the breaking and branching of the selenium chains. The total coordination number is shown to result from the balance between one-, two-, and threefold coordinated atoms. The role of these defects is discussed in relationship with the electrical conductivity of the liquid, i.e., the semiconductor-metal and metal-nonmetal transitions observed at high pressures [less ▲]

Detailed reference viewed: 10 (2 ULg)
Full Text
Peer Reviewed
See detailNear infrared spectroscopy in large animals: optical pathlengh and influence of hair covering and epidemal pigmentation
Pringle, J.; Roberts, C.; Kohl, M. et al

in Veterinary Journal (1999), 158

The effects of epidermal pigmentation and hair covering on the relative transparency of various animal tissues to near infrared (NIR) light were examined, and the pathlengths of NIR light through tissues ... [more ▼]

The effects of epidermal pigmentation and hair covering on the relative transparency of various animal tissues to near infrared (NIR) light were examined, and the pathlengths of NIR light through tissues at four wavelengths in the NIR range were subsequently determined. Black hair covering and black or dark-coloured hooves prevented NIR light from penetration sufficient for conduction of pathlength or NIR spectroscopy measurements. Non-pigmented hair covering of the head did not appear to be a barrier to successful NIR light transmission. Tissues sufficiently transparent to NIR light had the differential pathlength factor (DPF, i.e. the ratio of the observed light pathlength and the geometric light source-detector separation) of NIR light determined by intensity modulated spectroscopy at the wavelengths 744, 806, 834 and 860 nm. Horse gluteal muscles had DPFs of 6.2, 6.2, 6.0, and 5.6, whereas forelimb muscles had DPF of 4.7, 4.4, 4.5 and 3.9 at the respective wavelengths. Sheep heads had DPF of 7.2 +/- 0.3, 5.8 +/- 0.5, 5.5 +/- 0.4 and 4.4 +/- 0.6 (+/- SEM) for the above respective wavelengths, of which the pathlengths all differed significantly from the other, except for between 806 and 834 nm, and 834 and 860 nm. The DPF of horse hooves were 4.8 +/- 0.1, 4.8 +/- 0.1, 4.7 +/- 0.1 and 4.4 +/- 0.1 (SEM) for the above noted wavelengths, of which the pathlength at 744 and 806 nm differed from the pathlength at 860 nm (P>0.05). These results show that NIRS is possible through lighter pigmented hair and epidermal tissues, and provide DPFs of horse feet and muscle and the sheep head that enables quantitative NIRS in these species. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailLe cas clinique du mois. Rupture couverte d'un anevrysme infectieux post-typhique de l'aorte abdominale sous-renale.
SCHILS, Fabian ULg; SakalihasanN, Natzi ULg; Sente, J. M. et al

in Revue Médicale de Liège (1999), 54(10), 786-8

Mycotic aneurysms are uncommon but remain one of the most challenging clinical problems for the vascular surgeon. They are associated with high morbidity and mortality, and the clinical signs and symptoms ... [more ▼]

Mycotic aneurysms are uncommon but remain one of the most challenging clinical problems for the vascular surgeon. They are associated with high morbidity and mortality, and the clinical signs and symptoms are non specific. We report here the case of an abdominal aorta mycotic aneurysm due to salmonellosis treated by insertion of an arterial homograft. [less ▲]

Detailed reference viewed: 29 (5 ULg)
Full Text
Peer Reviewed
See detailAccounting for Environmental Influences in Stochastic Frontier Models: With Application to International Airlines
Coelli, Tim; Perelman, Sergio ULg; Romano, Elliot

in Journal of Productivity Analysis (1999), 11

The principal aim of this paper is to measure the efficiency of international airlines. We obtain measures of technical efficiency from stochastic frontier production functions which have been adjusted to ... [more ▼]

The principal aim of this paper is to measure the efficiency of international airlines. We obtain measures of technical efficiency from stochastic frontier production functions which have been adjusted to account for environmental influences such as network conditions, geographical factors, etc. We observe that two alternative approaches to this problem have been proposed in the efficiency measurement literature. One assumes that the environmental factors influence the shape of the technology while the other assumes that they directly influence the degree of technical inefficiency. In this paper we compare the results obtained when using these two approaches. The two sets of results provide similar rankings of airlines but suggest differing degrees of technical inefficiency. Both sets of results also suggest that Asian/Oceanic airlines are technically more efficient than European and North American airlines but that the differences are essentially due to more favourable environmental conditions. Nevertheless, it is among Asian companies that the major improvements in managerial efficiency (technical efficiency with environmental factors netted out) took place over the sample period (1977–1990). [less ▲]

Detailed reference viewed: 89 (7 ULg)
Full Text
Peer Reviewed
See detailLymphocytic colitis: a distinct clinical entity? A clinicopathological confrontation of lymphocytic and collagenous colitis.
Baert, F.; Wouters, K.; D'Haens, G. et al

in Gut (1999), 45(3), 375-81

BACKGROUND AND AIMS: It is not known whether lymphocytic colitis and collagenous colitis represent different clinical entities or constitute part of a spectrum of disease. METHODS: Detailed clinical ... [more ▼]

BACKGROUND AND AIMS: It is not known whether lymphocytic colitis and collagenous colitis represent different clinical entities or constitute part of a spectrum of disease. METHODS: Detailed clinical features and histological findings were compared in a large series of patients with confirmed lymphocytic and collagenous colitis. RESULTS: Histological diagnosis was confirmed in 96 patients with collagenous colitis and 80 with lymphocytic colitis. Twenty eight per cent of patients with collagenous colitis and 26% of patients with lymphocytic colitis had overlapping but less pronounced histological features. Both groups were equal in terms of age, use of aspirin and non-steroidal anti-inflammatory drugs, associated autoimmune conditions, arthritis, diarrhoea, and abdominal pain. The male:female ratio was 27:73 for collagenous colitis and 45:55 for lymphocytic colitis (p=0.013). Twenty five per cent of patients with collagenous colitis compared with 14% of patients with lymphocytic colitis were active smokers; only 8.3% of patients with collagenous colitis had stopped smoking compared with 23% of patients with lymphocytic colitis (p=0.013). Drug induced disease was suspected for ticlopidine (two collagenous colitis, four lymphocytic colitis) and flutamide (four lymphocytic colitis). Mean duration of symptoms before diagnosis was two months for lymphocytic colitis and four months for collagenous colitis. Overall prognosis was generally mild; 84% of patients with lymphocytic colitis and 74% of patients with collagenous colitis reported resolution or significant improvement (p=0.033). CONCLUSIONS: Collagenous and lymphocytic colitis are similar but not identical. Patients with lymphocytic colitis present somewhat earlier and are less likely to be active smokers. Symptoms are milder and more likely to disappear in lymphocytic colitis. Ticlopidine and flutamide should be added to the list of drugs inducing colitis. [less ▲]

Detailed reference viewed: 34 (1 ULg)
Full Text
Peer Reviewed
See detailComputer Simulation Of Surfactin Conformation At A Hydrophobic/Hydrophilic Interface
Gallet, X.; Deleu, Magali ULg; Razafindralambo, Hary ULg et al

in Langmuir (1999), 15(7), 2409-13

Using a molecular modeling method, different conformations of surfactin at a hydrophobic/hydrophilic interface are established. Two conformations of the peptide ring (S1 and S2) provided by NMR ... [more ▼]

Using a molecular modeling method, different conformations of surfactin at a hydrophobic/hydrophilic interface are established. Two conformations of the peptide ring (S1 and S2) provided by NMR experiments built with three different aliphatic chains in folded or extended configurations were studied. For the structures including the S2 peptide ring conformation, the theoretical interfacial molecular area corresponds to the experimental limiting area A0 value obtained with a Langmuir film balance. The peptide ring is positioned in the plane of the interface with the two acidic chains close to each other and protruding in the aqueous phase, and the β-hydroxy fatty acid chain, folded to interact mainly with the Leu2 side chain and also with the Val4 side chain. This design has the largest calculated molecular area and would correspond to the most stable amphipathic structure representing the surfactin experimental behavior in weak compression. [less ▲]

Detailed reference viewed: 19 (0 ULg)
Full Text
Peer Reviewed
See detailInfluence function and efficiency of the minimum covariance determinant scatter matrix estimator
Croux, C.; Haesbroeck, Gentiane ULg

in Journal of Multivariate Analysis (1999), 71(2), 161-190

The minimum covariance determinant (MCD) scatter estimator is a highly robust estimator for the dispersion matrix of a multivariate, elliptically symmetric distribution. It is relatively fast to compute ... [more ▼]

The minimum covariance determinant (MCD) scatter estimator is a highly robust estimator for the dispersion matrix of a multivariate, elliptically symmetric distribution. It is relatively fast to compute and intuitively appealing. In this note we derive its influence function and compute the asymptotic variances of its elements. A comparison with the one step reweighted MCD and with S-estimators is made. Also finite-sample results are reported. (C) 1999 Academic Press AMS 1991 subject classifications: 62F35, 62G35. [less ▲]

Detailed reference viewed: 36 (6 ULg)
Full Text
Peer Reviewed
See detailAirway epithelial cell migration dynamics. MMP-9 role in cell-extracellular matrix remodeling
Legrand, C.; Gilles, Christine ULg; Zahm, J. M. et al

in Journal of Cell Biology (1999), 146(2), 517-29

Cell spreading and migration associated with the expression of the 92-kD gelatinase (matrix metalloproteinase 9 or MMP-9) are important mechanisms involved in the repair of the respiratory epithelium. We ... [more ▼]

Cell spreading and migration associated with the expression of the 92-kD gelatinase (matrix metalloproteinase 9 or MMP-9) are important mechanisms involved in the repair of the respiratory epithelium. We investigated the location of MMP-9 and its potential role in migrating human bronchial epithelial cells (HBEC). In vivo and in vitro, MMP-9 accumulated in migrating HBEC located at the leading edge of a wound and MMP-9 expression paralleled cell migration speed. MMP-9 accumulated through an actin-dependent pathway in the advancing lamellipodia of migrating cells and was subsequently found active in the extracellular matrix (ECM). Lamellipodia became anchored through primordial contacts established with type IV collagen. MMP-9 became amassed behind collagen IV where there were fewer cell-ECM contacts. Both collagen IV and MMP-9 were involved in cell migration because when cell-collagen IV interaction was blocked, cells spread slightly but did not migrate; and when MMP-9 activation was prevented, cells remained fixed on primordial contacts and did not advance at all. These observations suggest that MMP-9 controls the migration of repairing HBEC by remodeling the provisional ECM implicated in primordial contacts. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailL'ostéoarthrose chez le chien - Pathophysiologie et diagnostic
Loneux, P.; Balligand, Marc ULg

in Annales de Médecine Vétérinaire (1999), (143), 153-160

Detailed reference viewed: 13 (2 ULg)
Full Text
Peer Reviewed
See detailFrom a New-World Poetics to a New-World Vision: African America in the Works of Edouard Glissant and Caryl Phillips
Ledent, Bénédicte ULg

in Commonwealth : Essays and Studies (1999), 21(2), 29-36

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailLa transmission du virus de l’hépatite C en milieu hospitalier
Delwaide, Jean ULg; Gerard, Christiane ULg; Belaiche, Jacques ULg et al

in Médecine et Hygiène (1999), 57

Detailed reference viewed: 33 (8 ULg)
Full Text
Peer Reviewed
See detailDerived Projective Limits of Topological Abelian Groups
Prosmans, Fabienne ULg

in Journal of Functional Analysis (1999), 162(1), 135-177

We prove that the category of topological Abelian groups is quasi-Abelian. Using results about derived projective limits in quasi-Abelian categories, we study exactness properties of the projective limit ... [more ▼]

We prove that the category of topological Abelian groups is quasi-Abelian. Using results about derived projective limits in quasi-Abelian categories, we study exactness properties of the projective limit functor in the category of topological Abelian groups. [less ▲]

Detailed reference viewed: 19 (3 ULg)
Full Text
Peer Reviewed
See detailSome phase equilibrium constraints on the origin of Proterozoic (massif) anorthosites and related rocks
Longhi, John ULg; Vander Auwera, Jacqueline ULg; Fram, Miranda S. et al

in Journal of Petrology (1999), 40

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailImpaired motor coordination and Purkinje cell excitability in mice lacking calretinin
Schiffmann, Serge N.; Cheron, Guy; Lohoff, Ann et al

in Proceedings of the National Academy of Sciences of the United States of America (1999), 96

n the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium ... [more ▼]

n the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca2+]i). Ca2+ binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr-/-) are impaired in tests of motor coordination. An impairment in Ca2+ homeostasis in Cr-/- Purkinje cells was supported by the high Ca2+-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr-/- alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology [less ▲]

Detailed reference viewed: 23 (4 ULg)
Peer Reviewed
See detailParticularités de la contraction électro-induite : implications rééducatives
Vanderthommen, Marc ULg; Zahlan, O.; BAUVIR, Philippe ULg et al

in Kine 2000 (1999), 10

Detailed reference viewed: 23 (7 ULg)
Peer Reviewed
See detailLymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
Coumans, Bernard ULg; Thellin, Olivier ULg; Zorzi, Willy ULg et al

in Journal of Immunological Methods (1999), 224(1-2), 185-196

To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts ... [more ▼]

To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 36 (4 ULg)
Full Text
Peer Reviewed
See detailHyperfractionated accelerated radiotherapy (HART) for inoperable, non metastatic non-small-cell lung carcinoma of the lung (NSCLC): result of a phase II study for patients ineligible for combination radio-chemotherapy.
Koutaissoff, S; Wellmann, D; COUCKE, Philippe ULg et al

in International Journal of Radiation, Oncology, Biology, Physics (1999), 45(5), 1151-1156

Purpose: To evaluate a hyperfractionated and accelerated radiotherapy (HART) protocol in patients with inoperable non-small cell lung carcinoma (NSCLC) who were ineligible for combination ... [more ▼]

Purpose: To evaluate a hyperfractionated and accelerated radiotherapy (HART) protocol in patients with inoperable non-small cell lung carcinoma (NSCLC) who were ineligible for combination radiochemotherapy studies. Methods and Materials: From February 1989 through August 1994, 23 patients ineligible for available combined modality protocols in our institution were enrolled and treated with HART, consisting of 63 Gy given in 42 fractions of 1.5 Gy each, twice daily, with a minimum time interval of 6 h between fractions, 5 days a week, over an elapsed time of 4.2 weeks, or 29 days. There was no planned interruption. Results: The 1-, 2-, and 3-year survival rates were 61%, 39%, and 19%, respectively, with a median survival of 16.8 months. At the time of analysis, 4 patients are alive and 19 have died, 16 from NSCLC and 3 from cardiacdisease. Overall response rate was 48%, with 22% of patients achieving a complete response and 26% a partial response. Correlation between acute response rate and survival was poor. First site of relapse was local-regional in 8 patients (35%), distant in 6 patients (26%), and local-regional and distant in 4 (17%) patients. One patient had Grade IV and 2 had Grade III esophagitis. One patient presented with chronic Grade III lung toxicity. There were no treatment-related deaths. Conclusion: In this group of 23 patients ineligible for radiochemotherapy, this HART regime was quite feasible and was followed by little toxicity. Results in this particularly poor prognosis NSCLC patient category should be compared to series with a similar patient profile; however, median survival is at least similar to that obtained in recent series of combination radiochemotherapy. © 1999 Elsevier Science Inc. [less ▲]

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailChanges in mediobasal hypothalamic gonadotropin-releasing hormone messenger ribonucleic acid levels induced by mating or ovariectomy in a reflex ovulator, the ferret.
Bakker, Julie ULg; Rubin, B. S.; Baum, M. J.

in Endocrinology (1999), 140(2), 595-602

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large ... [more ▼]

The ferret is a reflex-ovulating species in which receipt of an intromission induces a prolonged (+/- 12 h) preovulatory LH surge in the estrous female. This LH surge is probably stimulated by a large release of GnRH from the mediobasal hypothalamus (MBH). In Exp 1 we asked whether GnRH messenger RNA (mRNA) levels increase in response to mating so as to replenish the MBH GnRH stores needed to sustain the preovulatory LH surge. Estrous females were killed 0, 0.25, 0.5, 1, 3, 6, 14, or 24 h after the onset of a 10-min intromission from a male. Coronal brain sections ranging from the rostral preoptic area caudally to the posterior hypothalamus were processed for in situ hybridization using a 35S-labeled oligoprobe complementary to the human GnRH-coding region. We found no evidence of increased MBH GnRH mRNA levels during the ferret's mating-induced preovulatory LH surge. Instead, the number of GnRH mRNA-expressing cells dropped significantly in the arcuate region beginning 6 h after onset of intromission and remained low thereafter. Furthermore, cellular GnRH mRNA levels decreased in the arcuate region toward the end of the preovulatory LH surge. In Exp 2 we asked whether ovarian hormones regulate MBH GnRH mRNA levels in the female ferret. Ovariectomy of estrous females significantly reduced the number of GnRH mRNA-expressing cells in the arcuate region. This decrease was probably not due to the absence of circulating estradiol. Gonadally intact anestrous females had levels of MBH GnRH mRNA similar to those in estrous females even though plasma estradiol levels were equally low in anestrous females and ovariectomized females. Ovarian hormones other than estradiol may stimulate MBH GnRH mRNA levels in anestrous and estrous females. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detailHas disinfection of endoscopes by automatic machines become obligatory?
Delwaide, Jean ULg; Pelckmans, P.; Defrance, P. et al

in Revue Médicale de Bruxelles (1999), 20(1), 49-50

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailThe ribonucleoside diphosphate reductase inhibitor (E)-2'-Deoxy-(fluoromethylene) cytidine, acts as a cytotoxic radiosensitizer on human cancer cell lines in vitro.
Coucke, Philippe ULg; Decosterd, L-A; Li, Y-X et al

in Cancer Research (1999), 59

ABSTRACT (E)-2*-Deoxy-(fluoromethylene)cytidine (FMdC) is known as an inhibitor of ribonucleoside diphosphate reductase, a key enzyme in the de novo pathway of DNA synthesis. FMdC was tested as a modifier ... [more ▼]

ABSTRACT (E)-2*-Deoxy-(fluoromethylene)cytidine (FMdC) is known as an inhibitor of ribonucleoside diphosphate reductase, a key enzyme in the de novo pathway of DNA synthesis. FMdC was tested as a modifier of radiation response in vitro on a human colon carcinoma cell line (WiDr), and the observed radiosensitization was confirmed on two human cervix cancer cell lines (C33-A and SiHa). Using the clonogenic assay, the effect ratio (ER) at a clinically relevant dose level of 2 Gy was 2.10 (50 nM FMdC), 1.70 (30 nM FMdC), and 1.71 (40 nM FMdC) for the three cell lines WiDr, C33-A, and SiHa, respectively. A more detailed analysis of the importance of timing and concentration of FMdC was done on the WiDr cell line alone, yielding an increased ER(2Gy) with increasing concentration and duration of exposure to the drug, ranging from 1.0 (6 h) to 1.8 (72 h) at 30 nM FMdC and from 1.2 (6 h) to 3.5 (24 h) at 300 nM. We investigated the effect of FMdC on the cellular deoxynucleotide triphosphate pool in WiDr cells and demonstrated a marked depletion of dATP and a significant rise of TTP levels. Cell cycle analysis showed early S-phase accumulation induced by FMdC alone, G2-M block induced by irradiation alone, and an increased accumulation of cells in G2-M if both modalities are used. Our data suggest that FMdC is a radiation response modifier in vitro on different cancer cell lines. The observed radiosensitization may in part be explained by alteration of the deoxynucleotide triphosphate pool, which is consistent with the effect of FMdC on ribonucleoside diphosphate reductase. [less ▲]

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailNonprofit Organisations and Social Economy: Two Ways of Understanding the Third Sector
Mertens de Wilmars, Sybille ULg

in Annals of Public and Cooperative Economics = Annales de l'Economie Publique, Sociale et Coopérative (1999), 70:3

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by ... [more ▼]

The term third sector refers to a set of organizations which are neither capitalist, nor governmental. First of all, this paper aims to extent B. Gui's unitary approach to the third-sector (1991) by focusing on the distinction between third sector and state-owned bodies. Next I propose to translate the non capitalist and the non governmental conditions into operational criteria. I show that these criteria vary according to the nature of the organizations (general interest versus mutual interest). This statement allows me to justifiy the emergence of two different ways of understanding the third-sector in anglo-saxon and french-speaking traditions. [less ▲]

Detailed reference viewed: 81 (5 ULg)
Peer Reviewed
See detailL'etude clinique du mois. Interet d'augmenter un taux de cholesterol HDL bas en prevention secondaire de la coronaropathie: resultats de l'etude VA-HIT.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(9), 773-5

The "Veterans Affairs Cooperative Studies Program High-Density Lipoprotein Cholesterol Intervention Trial" (VA-HIT) is a large randomised, double-blind, placebo-controlled clinical trial for the secondary ... [more ▼]

The "Veterans Affairs Cooperative Studies Program High-Density Lipoprotein Cholesterol Intervention Trial" (VA-HIT) is a large randomised, double-blind, placebo-controlled clinical trial for the secondary prevention of coronary heart disease. It demonstrates that a fibrate treatment (gemfibrozil) significantly reduces the relative risk of major coronary (-22%, p = 0.006) and cardiovascular (-24%, p < 0.001) events in men with coronary heart disease whose primary lipid abnormality is a low HDL cholesterol level. These findings suggest that, in such a population, the rate of coronary events is reduced under gemfibrozil therapy by raising HDL cholesterol levels (+6%) and lowering levels of triglycerides (-31%) without lowering LDL cholesterol concentrations. [less ▲]

Detailed reference viewed: 41 (0 ULg)
Peer Reviewed
See detailTheophylline Decreases Sputum Eosinophilia of Asthmatics
Louis, Renaud ULg; Bettiol, J.; Cataldo, Didier ULg et al

in International Archives of Allergy & Immunology (1999), 118(2-4, Feb-Apr), 343-4

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailFlow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet
Kevers, Claire ULg; Greimers, Roland ULg; Franck, Thierry ULg et al

in Biologia Plantarum (1999), 42(3), 321-332

A fully habituated (auxin- and cytokinin-independent) self-regenerating (organogenic) sugar beet cell line (HO) and a fully habituated non-organogenic one (HNO) derived from the former one, were analyzed ... [more ▼]

A fully habituated (auxin- and cytokinin-independent) self-regenerating (organogenic) sugar beet cell line (HO) and a fully habituated non-organogenic one (HNO) derived from the former one, were analyzed as to their nuclear size and DNA content. Flow cytometry and image analysis were used and cells of certified diploid leaves of the same sugar beet strain served as controls. The HNO cells had been shown previously to have many characteristics of cancerous cells. The analyses made on leaves and HNO cells indicated the presence of only one population of cycling cells. In HO cells. two cycling populations were detected: the first one had the same DNA content as the leaves while the second one contained two fold more DNA than the first population. HNO cells showed the higher nuclear size and DNA content. HNO cells also showed evidence of aneuploidy. Thus, nuclear size, DNA content and ploidy level increase together with the neoplasic progression to culminate in HNO cells with the loss of organogenic totipotency. [less ▲]

Detailed reference viewed: 61 (3 ULg)
Full Text
Peer Reviewed
See detailClinical use of a bioartificial liver to treat acetaminophen-induced fulminant hepatic failure
Detry, Olivier ULg; Arkadopoulos, N.; Kahaku, E. et al

in American Surgeon (1999), 65

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailAnnual activity cycle of adult brown trout (Salmo trutta L.): A radio-telemetry study in a small stream of the Belgian Ardenne
Ovidio, Michaël ULg

in Bulletin Français de la Pêche et de la Pisciculture (1999), (352), 1-18

During a study period of 26 months, twenty trout (26.0-57.0 cm FL; 198-1,685 g) were daily located from 16 to 466 days in a small stream of the Belgian Ardenne, the Aisne stream (tributary of the river ... [more ▼]

During a study period of 26 months, twenty trout (26.0-57.0 cm FL; 198-1,685 g) were daily located from 16 to 466 days in a small stream of the Belgian Ardenne, the Aisne stream (tributary of the river Ourthe) in order to characterize their annual pattern of mobility. Daily movements were more frequent and longer during the spawning period (October-December) than at any other time of the year. Upstream migrations (max.: 25 km) generally occurred during October and the first fortnight of November and were triggered by a combination of Variations of water temperature and water level within a thermal range of 10-14 degrees C. Spawning activity (second fortnight of November until late December) took place in the Aisne stream (max. width : 10 m) or in its small tributaries. Trout surviving spawning undertook a fast (max.:9,200 m in 24 h) and precise post reproductive homing. From winter to summer, daily movements were shorter and mainly corresponded to changes of residences within a home range of which the size was proportional to the trout's size. In March and June, some trout made long upstream unidirectional migrations of which the biological signification is still unknown. These results are discussed within the context of life history strategies and management of trout populations. [less ▲]

Detailed reference viewed: 163 (23 ULg)
Peer Reviewed
See detailL'homeopathie est-elle superieure au placebo? Controverse a propos d'une meta-analyse des etudes controlees.
Scheen, André ULg; Lefebvre, Pierre ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(7-9), 295-304304-7

At a time when scientists support Evidence-Based Medicine, the Parliament of Belgium has recently decided to recognize four alternative medicines, among which homeopathy. Whereas this discipline does not ... [more ▼]

At a time when scientists support Evidence-Based Medicine, the Parliament of Belgium has recently decided to recognize four alternative medicines, among which homeopathy. Whereas this discipline does not rely on any scientific basis, it appears to be popular, especially in general practice. The homeopaths have recently taken arguments from a meta-analysis published in 1997 in the Lancet of 89 placebo-controlled trials. This study indeed concluded that the results are not compatible with the hypothesis that the clinical effects of homeopathy are completely due to placebo. However, this meta-analysis contains several methodological flaws. Furthermore, it is recognized that results of a meta-analysis may not be confirmed in large well-performed clinical trials. Thus, homeopathy should still provide the evidence that conventional medicine has regularly brought during the last two decades in many fields of therapeutics, with the respect of the rigorous rules of "Good Clinical Practice", leading to "Evidence-Based Medicine". [less ▲]

Detailed reference viewed: 251 (10 ULg)
Full Text
Peer Reviewed
See detailStructure and functional analysis of a tilapia (Oreochromis mossambicus) growth hormone gene: activation and repression by pituitary transcription factor Pit-1
Sekkali, Belaid; Brim, Hassan; Muller, Marc ULg et al

in DNA & Cell Biology (1999), 18(6), 489-502

A gene encoding the Tilapia mossambica (Oreochromis mossambicus) growth hormone (tiGH) was isolated and sequenced. The gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences and a 1 ... [more ▼]

A gene encoding the Tilapia mossambica (Oreochromis mossambicus) growth hormone (tiGH) was isolated and sequenced. The gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences and a 1.7-kb transcription unit comprised of six exons and five introns. The gene and the 5' flanking region contain several potential binding sites for Pit-1, a key transcription activator of mammalian GH genes. One of these (-57/-42) is highly conserved in fish GH genes. It activates transcription in pituitary cells and binds Pit-1. Transfection of luciferase reporter plasmids containing either the -3602/+19 tiGH sequence or one of its 5' deletion mutants (-2863/, -1292/, and -463/+19) resulted in strong activity in Pit-1-producing rat pituitary GC cells. A dose-dependent activation of the tiGH promoter was achieved in nonpituitary fish EPC and monkey COS cells cotransfected with a rat Pit-1 expression vector, demonstrating the crucial role played by Pit-1 as an activator of the tiGH gene. Fusion of the tiGH promoter with the beta-galactosidase gene led to transient expression specifically in the nervous system of microinjected zebrafish embryos. The activity of the tiGH promoter in GC and EPC cells was strongly repressed by extending its 3' end from +19 to +40, a sequence in which a Pit-1-binding site was identified using gel retardation assays. Point mutations of the site that suppressed Pit-1 binding in vitro restored full tiGH promoter activity. Thus, a Pit-1-binding site located in the 5' untranslated region mediates Pit-1-dependent repression of the tiGH gene. [less ▲]

Detailed reference viewed: 40 (5 ULg)
Full Text
Peer Reviewed
See detailValue of FDG-PET in detecting residual or recurrent nonsmall cell lung cancer
Bury, Thierry ULg; Corhay, Jean-Louis ULg; Duysinx, Bernard ULg et al

in European Respiratory Journal (1999), 14(6), 1376-1380

In order to evaluate the usefulness of 18-fluorodeoxyglucose (FDG) positron emission tomography (PET) in the assessment of therapeutic effects, a study was performed before and after therapy in 126 ... [more ▼]

In order to evaluate the usefulness of 18-fluorodeoxyglucose (FDG) positron emission tomography (PET) in the assessment of therapeutic effects, a study was performed before and after therapy in 126 patients with non-small cell lung cancer (NSCLC) codified stage I to stage IIIB. Treatment with an early curative result was given in 58 patients, whereas in 68 cases it was limited to palliation. During the treatment follow-up period (8-40 months), each patient was evaluated every 3 months by clinical examination and ≤6 months by imaging techniques (PET and computed tomography (CT)). A diagnosis of persistent or recurrent tumour was established by means of pathological analysis in 31 patients and by clinical evolution and subsequent imaging progression in 29 other patients. PET showed increased FDG uptake in all cases (n=60) of persistent or recurrent tumour, whereas CT was nonspecific in 17 cases. Conversely, there were five false positive cases via PET imaging and three via CT. In detecting residual or recurrent NSCLC, PET had a sensitivity of 100% and specificity of 92%, whereas CT had a sensitivity and specificity of 71% and 95% respectively. In conclusion, 18-fluorodeoxyglucose positron emission tomography correctly identified response to therapy in 96% (121 of 126) of patients. Positron emission tomography appears to be more accurate (p=0.05) than conventional imaging in distinguishing persistent or recurrent tumour from fibrotic scar in patients undergoing treatment for non-small cell lung cancer. [less ▲]

Detailed reference viewed: 49 (7 ULg)
Full Text
Peer Reviewed
See detailImpacts of (NH4)2SO4 deposition on Norway spruce (Picea abies [L.] Karst) roots
Carnol, Monique ULg; Cudlin, Pavel; Ineson, Phil

in Water, Air & Soil Pollution (1999), 116

The effects of enhanced (NH4)(2)SO4 (NS) deposition on Norway spruce (Picea abies [L.] Karst) fine root biomass, vitality and chemistry were investigated using root-free in-growth cores reproducing native ... [more ▼]

The effects of enhanced (NH4)(2)SO4 (NS) deposition on Norway spruce (Picea abies [L.] Karst) fine root biomass, vitality and chemistry were investigated using root-free in-growth cores reproducing native organic and mineral soil horizons. The cores were covered and watered every 2 weeks with native throughfall or throughfall supplemented with NS to increase deposition by 75 kg ha(-1) a(-1) NH4+-N (86 kg ha(-1) a(-1) SO42--S). The in-growth cores were sampled after 19 months and assessed for root biomass, necromass, length, tip number, tip vitality and fine root chemistry. Root biomass and fine root aluminium (Al) concentration were negatively correlated, but NS deposition had no effect on root growth or root tip vitality. NS deposition caused increased fine root nitrogen (N) concentrations in the organic horizon and increased Calcium (Ca) concentrations in the mineral horizon. Fine root biomass was higher in the organic horizon, where fine root Al and potassium (K) concentrations were lower and Ca concentrations higher than in the mineral horizon. Results highlighted the importance of soil stratification on fine root growth and chemical composition. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailInfo-congres. L'etude HOPE: demonstration de la protection cardio-vasculaire du ramipril, un inhibiteur de l'enzyme de conversion de l'angiotensine.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(10), 835-6

The results of the HOPE ("Heart Outcomes Prevention Evaluation") study were presented at the XXIst Congress of the European Society of Cardiology in Barcelona (August 28-September 1, 1999). In addition ... [more ▼]

The results of the HOPE ("Heart Outcomes Prevention Evaluation") study were presented at the XXIst Congress of the European Society of Cardiology in Barcelona (August 28-September 1, 1999). In addition, results obtained in the large diabetic sub-population (38% of the 9541 patients included in the trial) were reported at the XXXVth Congress of the European Association for the Study of Diabetes in Brussels (September 28-October 2, 1999). The HOPE trial was terminated early because of a reduction of the relative risk (-22%, p < 0.000002) of major cardiovascular events in the group treated with ramipiril compared to the group receiving placebo. Such a favourable effect of ramipril was observed in non-diabetic subjects in secondary prevention and in diabetic patients in secondary or primary prevention. In the latter population, a significant reduction in the incidence of microangiopathic complications (nephropathy and retinopathy) was also demonstrated. [less ▲]

Detailed reference viewed: 55 (0 ULg)
Peer Reviewed
See detailPrise en charge médico-chirurgicale des enfants cardiopathes: résultats de l'expérience liégeoise
Massin, M.M.; RADERMECKER, Marc ULg; Jalali, H. et al

in Revue Médicale de Liège (1999), 54(2), 95-9

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and ... [more ▼]

OBJECTIVE: Evaluation of the results obtained in the management of congenital heart diseases in Liege. MATERIAL AND METHODS: We evaluated the results, the mortality and the morbidity of diagnostic and interventional cardiac catheterization, and of the cardiac surgery in the 123 cardiac children who were referred to us during the three first years of operation of our medico-surgical team. RESULTS: 89 children underwent a catheterization, including 12 therapeutic interventions, whereas 68 were operated. In the two domains, the results are completely comparable with those of the established centers. CONCLUSIONS: The widening of the activity of paediatric cardiology and the creation of an activity of pediatric cardiac surgery in Liege appeared important to us for the quality of management of the cardiac children. This goal could be reached only if our results were comparable with those of the literature. The bet seems to be held. The ultimate objective is to contribute by an adequate policy of management to the quality of life and the health of the cardiac children. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailEGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation.
Du, G.; Willet, K.; Mouithys-Mickalad, Ange ULg et al

in Free Radical Biology & Medicine (1999), 27

The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to ... [more ▼]

The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 +/- 0.11) decreased after anoxia/reoxygenation (1.75 +/- 0.09, p < .01), as well as state 3 and uncoupled respiration (-20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50-200 microg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 microg/ml EGb 761, they showed an increase in ADP/O (2.09 +/- 0.14, p < .05) and a decrease in state 4 respiration (-22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailHemodynamic changes and catecholamine release during laparoscopic adrenalectomy for pheochromocytoma.
Joris, Jean ULg; Hamoir, Etienne ULg; Hartstein, Gary ULg et al

in Anesthesia and Analgesia (1999), 88(1), 16-21

We investigated hemodynamics and plasma catecholamine concentrations in eight consecutive patients undergoing laparoscopic adrenalectomy for suspected pheochromocytoma. The same anesthesia protocol was ... [more ▼]

We investigated hemodynamics and plasma catecholamine concentrations in eight consecutive patients undergoing laparoscopic adrenalectomy for suspected pheochromocytoma. The same anesthesia protocol was used in all patients: a continuous infusion of sufentanil 0.5 microg x kg(-1) x h(-1) and isoflurane 0.4% (end-tidal) in 50% N2O/O2. Systolic arterial pressure was maintained between 120 and 160 mm Hg by adjusting an infusion of nicardipine, a calcium-channel blocker, while tachycardia (>100 bpm) was treated by 1-mg boluses of atenolol. Hemodynamics (thermodilution technique) and plasma catecholamine concentrations were measured before surgery, after the induction of anesthesia, after turning the patient to the lateral position, during pneumoperitoneum, during tumor manipulation, after adrenalectomy, and at the end of surgery. Two events resulted in significant catecholamine release: creation of the pneumoperitoneum and adrenal gland manipulation. As a consequence, a twofold increase in cardiac output was recorded. Adjustments of nicardipine infusion (2-6 microg x kg(-1) x min(-1)) minimized changes in mean arterial pressure. Beta-adrenergic blockade was necessary in six patients. In conclusion, laparoscopic adrenalectomy for pheochromocytoma results in marked catecholamine release during pneumoperitoneum and tumor manipulation. Titration of a nicardipine infusion allowed easy and quick control of the hemodynamic aberrancies related to these processes. IMPLICATIONS: Pneumoperitoneum during laparoscopy, now used for adrenalectomy, may complicate anesthetic management of patients with pheochromocytoma. In this study, laparoscopic adrenalectomy was associated with catecholamine release during the creation of pneumoperitoneum and tumor manipulation. Adjustments of a nicardipine infusion readily attenuated the subsequent hemodynamic aberrancies. [less ▲]

Detailed reference viewed: 107 (22 ULg)
Peer Reviewed
See detailThiazolidinediones.
Scheen, André ULg; Paquot, Nicolas ULg; Letiexhe, Michel ULg et al

in Journées Annuelles de Diabetologie de l'Hôtel-Dieu (1999)

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailNew evaluation therapies for sepsis.
Deby; Damas, Pierre ULg; Lamy, Maurice ULg

in Current Opinion in Anaesthesiology (1999), 12(2), 121-7

This article presents an analysis of the literature of the last 2 years on the treatment of sepsis and septic shock. It appears that most of the antimediator therapies applied in phase II or III clinical ... [more ▼]

This article presents an analysis of the literature of the last 2 years on the treatment of sepsis and septic shock. It appears that most of the antimediator therapies applied in phase II or III clinical trials yielded disappointing results. But, many other therapeutic approaches are proposed or are ongoing, and many papers report critical analysis of the reasons for the lack of success of past clinical trials. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailPlatyhypnidium mutatum, a mysterious new moss from Germany
Ochyra, R.; Vanderpoorten, Alain ULg

in Journal of Bryology (1999), 21

Detailed reference viewed: 26 (2 ULg)
Peer Reviewed
See detailMadangolide and Laingolide A, Two Novel Macrolides from Lyngbya bouillonii (Cyanobacteria)
Klein, D.; Daloze, D.; Hoffmann, L. et al

in Journal of Natural Products (1999)

Two new macrolide derivatives, madangolide (2) and laingolide A (3), have been isolated from the cyanobacterium Lyngbya bouillonii, collected in Papua New Guinea. Their structures (without stereochemistry ... [more ▼]

Two new macrolide derivatives, madangolide (2) and laingolide A (3), have been isolated from the cyanobacterium Lyngbya bouillonii, collected in Papua New Guinea. Their structures (without stereochemistry) have been established by detailed high-field 1D and 2D NMR studies and, in the case of 3, by comparison with the spectroscopic data of laingolide (1), previously isolated from the same organism. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailLes etudes cliniques controlees, support incontournable de la medecine basee sur l'evidence.
Scheen, André ULg; Kulbertus, Henri ULg

in Revue Médicale de Liège (1999), 54(1), 1

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailApplication and performances of graphitic chromium cast iron rolls
Lecomte-Beckers, Jacqueline ULg; Terziev, L.; Breyer, J. P. et al

in Revue de Métallurgie [=Revue de Métallurgie. Cahiers d'Informations Techniques] (1999), 96(11), 1401-1411

The main requirements for the development of a new grade for the later finishing section of the mill are : good oxidation and thermal behaviour, high wear resistance, good resistance to rolling incidents ... [more ▼]

The main requirements for the development of a new grade for the later finishing section of the mill are : good oxidation and thermal behaviour, high wear resistance, good resistance to rolling incidents. The approach of Marichal Ketin to improve the rolling performances in the last finishing stands is presented. The Hi-Cr cast iron possesses excellent wear resistance due to the presence of hard chromium carbides, but its thermal conductivity and sticking properties are fairly low. A graphite precipitation could contribute to an improvement in thermal conductivity and serve as lubricant preventing sticking. The new material, called graphitic chromium iron, shows good oxidation, wear and thermal behaviour allowing its use as work roll in the last stands of the finishing section of a HSM. In the as-cast condition, graphite precipitation in high Ni and Si chromium cast iron occurs at a temperature of 950 degrees C. Moreover, the amount and volume of free graphite may be increased by secondary graphite precipitation during suitable heat treatment. [less ▲]

Detailed reference viewed: 61 (2 ULg)
Full Text
Peer Reviewed
See detailHypoxia-induced activation of HIF-1: role of HIF-1alpha-Hsp90 interaction.
Minet, E.; Mottet, Denis ULg; Michel, G. et al

in FEBS Letters (1999), 460(2), 251-6

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear ... [more ▼]

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear translocator (ARNT)-Sim (PAS) factors Sim and aryl hydrocarbon receptor (Ahr). The transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha), involved in the response to hypoxia, also belongs to the bHLH-PAS family. This work was aimed to investigate the putative role of Hsp90 in HIF-1 activation by hypoxia. Using a EGFP-HIF-1alpha fusion protein, co-immunoprecipitation experiments evidenced that the chimeric protein expressed in COS-7 cells interacts with Hsp90 in normoxia but not in hypoxia. We also demonstrated that Hsp90 interacts with the bHLH-PAS domain of HIF-1alpha. Moreover, Hsp90 is not co-translocated with HIF-1alpha into the nucleus. At last, we showed that Hsp90 activity is essential for HIF-1 activation in hypoxia since it is inhibited in the presence of geldanamycin. These results indicate that Hsp90 is a major regulator in HIF-1alpha activation. [less ▲]

Detailed reference viewed: 50 (6 ULg)
Full Text
See detailOptimal acquisition of video images in reflected light microscopy
Pirard, Eric ULg; Lebrun, Vincent; Nivart, Jean-François

in European Microscopy and Analysis (1999), 60(9-11),

Detailed reference viewed: 44 (1 ULg)
Peer Reviewed
See detailMonoclonal Antibodies To Growth Hormone (Gh) Prolong Liver Gh Binding And Gh-Induced Igf-I/Igfbp-3 Synthesis
Beauloye, V.; Muaku, Sm.; Lause, P. et al

in American Journal of Physiology - Endocrinology and Metabolism (1999), 277(2),

Detailed reference viewed: 10 (4 ULg)
Peer Reviewed
See detailMedicaments et grossesse.
Scheen, André ULg

in Revue Médicale de Liège (1999), 54(5), 409-14

Since the dramatic observations of fetal abnormalities associated to thalidomide use during pregnancy, the risk of malformations when prescribing drugs in pregnant women is a well-known problem, among the ... [more ▼]

Since the dramatic observations of fetal abnormalities associated to thalidomide use during pregnancy, the risk of malformations when prescribing drugs in pregnant women is a well-known problem, among the public as well as among medical doctors. However, the complexity of the problem, the lack of robust scientific data and the emotional context characterizing this period make the prescription of medications to a pregnant woman a difficult task for every people involved in health care, the pharmacist, the general practitioner and even the gynaecologist. [less ▲]

Detailed reference viewed: 28 (2 ULg)
Full Text
See detailLa formation de formateurs en Communauté française de Belgique
Beckers, Jacqueline ULg

in Puzzle (1999), 6(juin), 21-22

Detailed reference viewed: 28 (3 ULg)
Full Text
Peer Reviewed
See detailSusceptibility of Bovine Antigen-Presenting Cells to Infection by Bovine Herpesvirus 1 and In Vitro Presentation to T Cells: Two Independent Events
Renjifo, Xiména; Letellier, Carine; Keil, Günther M. et al

in Journal of Virology (1999), 73

Detailed reference viewed: 8 (1 ULg)
See detailTell Amarna. Présentation sommaire de sept campagnes de fouilles (1991-1997)
Tunca, Önhan ULg

in Archaeology of the Upper Syrian Euphrates. The Tishrin Dam Area. Proceedings of the International Symposium Held at Barcelona (1999)

Detailed reference viewed: 26 (6 ULg)
Full Text
See detailDe Luikse regio: een gebied in verandering
Merenne-Schoumaker, Bernadette ULg

in De Aardrijkskunde (1999), (4), 3-44

Excusion booklet-guide in the Region of Liege with the aim to pin the principal changes occurred recently in the economy and within Liege spaces. Five parts : - a introduction presenting the program of ... [more ▼]

Excusion booklet-guide in the Region of Liege with the aim to pin the principal changes occurred recently in the economy and within Liege spaces. Five parts : - a introduction presenting the program of the excursion, - a short analysis of the town, - a recent assessment of the district econmy, - a general statistical file and - eleven thematic files devoted to eleven major points of the excusion [less ▲]

Detailed reference viewed: 34 (5 ULg)
Full Text
Peer Reviewed
See detailL’importance de l’emploi dans le secteur non marchand
Mertens de Wilmars, Sybille ULg; Marée, Michel ULg

in Non Marchand : Management, Droit et Finance (1999), (4), 11-27

Detailed reference viewed: 50 (2 ULg)
Peer Reviewed
See detailIdentification Of Cystosolic Mg2+-Dependent Soluble Inorganic Pyrophosphatases In Potato And Phylogenetic Analysis
Rojas-Beltran, Ja.; Dubois, Françoise ULg; Mortiaux, F. et al

in Plant Molecular Biology (1999), 39(3),

Detailed reference viewed: 26 (5 ULg)
Full Text
Peer Reviewed
See detailDo leaching experiments affect the Sm-Nd signature of deep-sea sediments? The example of the northern North Atlantic
Innocent, Christophe; Fagel, Nathalie ULg; Stevenson, Ross

in Canadian Journal of Soil Science (1999), 79

Detailed reference viewed: 27 (5 ULg)
Full Text
Peer Reviewed
See detailReproducibility of measurements of potential doubling time of tumor cells in the multicenter NCI protocol T92-0045
Wilson, G-D; Paschoud, N; Pavy, J-J et al

in British Journal of Cancer (1999), 79(2), 323-332

Summary We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary ... [more ▼]

Summary We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose was to understand and minimize the variation within the measurement. A total of 102 specimens were selected at random from patients entered into the trial. Samples were prepared, stained, run and analysed in each centre and a single set of data analysed by all three centres. Analysis of the disc data set revealed that the measurement of labelling index (LI) was robust and reproducible. The estimation of duration of S-phase ( Ts) was subject to errors of profile interpretation, particularly DNA ploidy status, and analysis. The LI dominated the variation in Tpot such that the level of final agreement, after removal of outliers and ploidy agreement, reached correlation coefficients of 0.9. The sample data showed poor agreement within each of the components of the measurement. There was some improvement when ploidy was in agreement, but correlation coefficients failed to exceed values of 0.5 for Tpot. The data suggest that observer-associated analysis of Ts and tissue processing and tumour heterogeneity were the major causes of variability in the Tpot measurement. The first two aspects can be standardized and minimized, but heterogeneity will remain a problem with biopsy techniques. [less ▲]

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailThe DmpA aminopeptidase from Ochrobactrum anthropi LMG7991 is the prototype of a new terminal nucleophile hydrolase family.
Fanuel, L; Goffin, Colette ULg; Cheggour, A et al

in Biochemical Journal (1999), 341(Pt 1), 147-55

The DmpA (d-aminopeptidase A) protein produced by Ochrobactrum anthropi hydrolyses p-nitroanilide derivatives of glycine and d-alanine more efficiently than that of l-alanine. When regular peptides are ... [more ▼]

The DmpA (d-aminopeptidase A) protein produced by Ochrobactrum anthropi hydrolyses p-nitroanilide derivatives of glycine and d-alanine more efficiently than that of l-alanine. When regular peptides are utilized as substrates, the enzyme behaves as an aminopeptidase with a preference for N-terminal residues in an l configuration, thus exemplifying an interesting case of stereospecificity reversal. The best-hydrolysed substrate is l-Ala-Gly-Gly, but tetra- and penta-peptides are also efficiently hydrolysed. The gene encodes a 375-residue precursor, but the active enzyme contains two polypeptides corresponding to residues 2-249 (alpha-subunit) and 250-375 (beta-subunit) of the precursor. Residues 249 and 250 are a Gly and a Ser respectively, and various substitutions performed by site-directed mutagenesis result in the production of an uncleaved and inactive protein. The N-terminal Ser residue of the beta-subunit is followed by a hydrophobic peptide, which is predicted to form a beta-strand structure. All these properties strongly suggest that DmpA is an N-terminal amidohydrolase. An exploration of the databases highlights the presence of a number of open reading frames encoding related proteins in various bacterial genomes. Thus DmpA is very probably the prototype of an original family of N-terminal hydrolases. [less ▲]

Detailed reference viewed: 11 (2 ULg)