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See detailInternational assessment of future low flow regimes and their impact on three water related sectors in the Meuse basin – a collaborative approach
Bauwens, Alexandra; Degré, Aurore ULg; Deraedt, Deborah ULg et al

in International Journal of River Basin Management (2015), 13(1), 123-135

There is a wide recognition of the watershed scale as the right scale for global water management, notably in the context of the WFD. Hence, it often refers to international management and therefore to ... [more ▼]

There is a wide recognition of the watershed scale as the right scale for global water management, notably in the context of the WFD. Hence, it often refers to international management and therefore to various pre-existing regional management tools, models or even objectives. In this study we aim at describing the collaborative assessment of climate change’s effect on low flow regime and the consequences on three water related sectors: drinking water production, agriculture and electricity production. The paper highlights the choices that were made during the study that involved scientific teams, managers and stakeholders from the four main countries of the Meuse Basin. It shows that the methodological choices were operational and aimed at preserving existing methods and knowledge within each country. They led to hydrological scenarios comparable to the main available ensemble approaches and to methodologies well accepted within the concerned countries. The results of the project highlight and quantify the water scarcity that the three sectors will have to face by the end of the century mainly regarding the electricity production. They also show that common allocation rules are necessary to manage water demand during future low flow periods. [less ▲]

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See detailExtracellular peptidase hunting for improvement of protein production in plant cells and roots
Lallemand, Jérôme ULg; Bouché, Frédéric ULg; Desiron, Carole et al

in Frontiers in Plant Science (2015), 6(37),

Plant-based recombinant protein production systems have gained an extensive interest over the past few years, because of their reduced cost and relative safety. Although the first products are now ... [more ▼]

Plant-based recombinant protein production systems have gained an extensive interest over the past few years, because of their reduced cost and relative safety. Although the first products are now reaching the market, progress are still needed to improve plant hosts and strategies for biopharming. Targeting recombinant proteins toward the extracellular space offers several advantages in terms of protein folding and purification, but degradation events are observed, due to endogenous peptidases. This paper focuses on the analysis of extracellular proteolytic activities in two production systems: cell cultures and root-secretion (rhizosecretion), in Arabidopsis thaliana and Nicotiana tabacum. Proteolytic activities of extracellular proteomes (secretomes) were evaluated in vitro against two substrate proteins: bovine serum albumin (BSA) and human serum immunoglobulins G (hIgGs). Both targets were found to be degraded by the secretomes, BSA being more prone to proteolysis than hIgGs. The analysis of the proteolysis pH-dependence showed that target degradation was mainly dependent upon the production system: rhizosecretomes contained more peptidase activity than extracellular medium of cell suspensions, whereas variations due to plant species were smaller. Using class-specific peptidase inhibitors, serine and metallopeptidases were found to be responsible for degradation of both substrates. An in-depth in silico analysis of genomic and transcriptomic data from Arabidopsis was then performed and led to the identification of a limited number of serine and metallo-peptidases that are consistently expressed in both production systems. These peptidases should be prime candidates for further improvement of plant hosts by targeted silencing. [less ▲]

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See detailRemote sensing enables high discrimination between organic and non-organic cotton for organic cotton certification in West Africa
Denis, Antoine ULg; Tychon, Bernard ULg

in Agronomy for Sustainable Development (2015)

One of the challenges of organic crop certification is the efficient targeting of the relatively small percentage of risk-sensitive fields that have to be controlled during the regulatory annual in situ ... [more ▼]

One of the challenges of organic crop certification is the efficient targeting of the relatively small percentage of risk-sensitive fields that have to be controlled during the regulatory annual in situ inspection. A previous study carried out on wheat and maize in Germany has shown that organic and non-organic crops can be efficiently distinguished by remote sensing. That study pointed to the possibility that these techniques could be used for helping organic crop certification bodies to better target risk-sensitive fields. This study is a first adaptation of that research on organic cotton in southwestern Burkina Faso, West Africa. This study assumed that organic and non-organic cotton, primarily because of their different approaches to fertilization and pest control, would result in bio-chemico-physical differences measurable by both in situ and remote sensing indicators. This study included 100 cotton fields, of which 50 were organic, 28 conventional, and 22 genetically modified. In situ indicators were derived from chlorophyll content, canopy cover, height, and spatial heterogeneity measurements. Remote sensing spectral and spatial heterogeneity indicators were derived from two SPOT 5 satellite images. Discriminant models were then computed. The results show statistically highly significant differences between organic and non-organic cotton fields for both in situ and satellite indicators, using univariate and multivariate linear models, with up to 86 % discrimination performance. This is the first time that the efficiency of using remote sensing to discriminate between organic and non-organic crops is evaluated in a developing country, particularly for cotton, with good discrimination being achieved. Pending further validation, it therefore seems that remote sensing could be used to enhance organic cotton certification in West Africa by enabling more efficient targeting of suspect fields and consequently could contribute to a better development of this sector. [less ▲]

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See detailReview on greenhouse gas emissions from pig houses: Production of carbon dioxide, methane and nitrous oxide by animals and manure
Philippe, François-Xavier ULg; Nicks, Baudouin ULg

in Agriculture, Ecosystems & Environment (2015)

The environmental impacts of livestock production are attracting increasing attention, especially the emission of greenhouse gases (GHGs). Currently, pork is the most widely consumed meat product in the ... [more ▼]

The environmental impacts of livestock production are attracting increasing attention, especially the emission of greenhouse gases (GHGs). Currently, pork is the most widely consumed meat product in the world, and its production is expected to grow in the next few decades. This paper deals with the production of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) by animals and by manure from pig buildings, with a focus on the influence of rearing techniques and nutrition. GHG emissions in piggeries originate from animals through CO2 exhalation and CH4 enteric fermentation, and from manure through the release of CO2, CH4 and N2O. The level of the CO2 exhalation (E-CO2, pig) depends on the physiological stage, the body weight (BW), the production level and the feed intake of the animals concerned. Enteric CH4 (E-CH4, pig) is principally related to dietary fibre intake and the fermentative capacity of the pig’s hindgut. Based on a review of the literature, the following equations are proposed in order to estimate E-CO2, pig (in kg day_1) and E-CH4,pig (in g day_1) for fattening pigs: E-CO2, pig = 0.136 _ BW0.573; E-CH4,pig = 0.012 _ dRes; with BW (in kg) and dRes for digestible residues (in g day_1). Numerous pathways are responsible for GHG production in manure. In addition, the microbial, physical and chemical properties of manure interact and modulate the level of emissions. Influencing factors for removal systems for both liquid and solid fractions of manure have been investigated. A large range of parameters showing an impact on the level of GHG production from pig houses has been reported. However, few of these can be considered unquestionably as GHG mitigation techniques because some strategies have shown contradictory effects depending on the gas, the circumstances and the study. Nevertheless, frequent manure removal seems to be an efficient means to reduce concurrently CO2-, CH4- and N2O-emissions from pig buildings for both slatted and bedded floor systems. Manure removal operations may be associated with specific storage conditions and efficient treatment in order to further reduce emissions. Several feeding strategies have been tested to decrease GHG emissions but they seem to be ineffective in reducing emissions both significantly and durably. In general, good management practices that enhance zootechnical performance will have beneficial consequences on GHG emission intensity. Taking into account the results described in the literature regarding CO2-, CH4- and N2O-production from animals and manure in pig houses, we estimate total GHG emissions to 448.3 kg CO2equiv. per slaughter pig produced or 4.87 kg CO2equiv. per kg carcass. The fattening period accounts for more than 70% of total emissions, while the gestation, lactation and weaning periods each contribute to about 10% of total emissions. Emissions of CO2, CH4 and N2O contribute to 81, 17 and 2% of total emissions from pig buildings, representing 3.87, 0.83 and 0.11 kg CO2equiv. per kg carcass, respectively. [less ▲]

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See detailQu'est-ce qu'un thème?
Badir, Sémir ULg

in Signata. Annales des Sémiotiques = Annals of Semiotics (2015), 5 (2014)

We approach the notion of theme as a problematic one. Particularly, one can observe that the functions of a theme, as they are assigned by the specialists, especially in linguistics and literary studies ... [more ▼]

We approach the notion of theme as a problematic one. Particularly, one can observe that the functions of a theme, as they are assigned by the specialists, especially in linguistics and literary studies, are multiple and mutually incompatible. It is from this variety reluctant to the disciplinarized modes of knowledge that we have in mind to establish the semiological nature of the theme. Its development is a knowledge that does not bend nor to analysis neither to synthesis. In this research, the lectures made by Roland Barthes at the Collège de France serve as a guide and as an example. [less ▲]

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See detailSartre in Venedig: der Mann, der in die Kälte ging
Cormann, Grégory ULg; Viehöver, Vera ULg

in Bulletin d'Analyse Phénoménologique (2015), 11

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See detailTentativi di avvicinamento a due mots-cible a partire da la zia d’america e il quarantotto : popolo e morale
Curreri, Luciano ULg

in Etica & Politica / Ethics & Politics (2015), XVII(1), 76-88

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See detailRituximab therapy for hairy cell leukemia : a retrospective study of 41 cases
Leclerc, Mathieu; Suarez, Felipe; Noël, Marie-Pierre et al

in Annals of Hematology (2015), 94

The purine analogs (PAs) cladribine and pentostatin have transformed the prognosis of hairy cell leukemia (HCL). However, some patients still relapse after PAs, or fail to reach an optimal response, and ... [more ▼]

The purine analogs (PAs) cladribine and pentostatin have transformed the prognosis of hairy cell leukemia (HCL). However, some patients still relapse after PAs, or fail to reach an optimal response, and new agents are needed to further improve treatment outcome. We retrospectively studied 41 HCL patients from 10 centers in France and Belgium, who received 49 treatment courses with the anti-CD20 monoclonal antibody rituximab. Most of the patients were treated at relapse (84 % of cases) and rituximab was combined to a PA in 41 % of cases. Overall, response rate is 90 % including 71 % complete hematologic responses (CHRs). Frontline treatment, combination therapy, and absolute neutrophil count were associated with response in multivariate analysis. Three-year relapse-free and overall survivals are 68 and 90 %, respectively. When combined to a PA, rituximab yields a 100 % response rate, even beyond frontline therapy. In contrast, response rate is only 82 % (59 % CHR) when rituximab is used alone. In this latter setting, relapse rate is 56 % and median time to relapse is 17.5 months. All eight patients who were treated two times with the antibody responded again to retreatment. We confirm the high efficacy of the combination rituximab + PA. However, when rituximab is used as monotherapy, response rate is lower and the high relapse rate is a concern. Prospective clinical trials are needed to confirm the superiority of the combination rituximab + PA over PA alone, both as frontline therapy and at relapse. [less ▲]

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See detailTowards a New Edition of the 'Répertoire métrique de la poésie des troubadours'
Valenti, Gianluca ULg

in Neophilologus (2015), 99(1), 15-27

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See detailEstimation of degree-days for different climatic zones of North-East India
Borah, Pallavi; Singh, Manoj Kumar; Mahapatra, Sadhan

in Sustainable Cities and Society (2015), 14

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See detailThe Koundoum sheep breed in Niger: morpho-biometric study and description of the production system
Hamadou, Issa; Moula, Nassim ULg; Siddo, Seyni et al

in Journal of Agriculture and Rural Development in the Tropics and Subtropics (2015), 116(1), 49-58

The diffusion of highly productive breeds across developing countries goes along with a neglect of indigenous breeds, which are well suited to their environment but often show low yields. Thus, in Niger ... [more ▼]

The diffusion of highly productive breeds across developing countries goes along with a neglect of indigenous breeds, which are well suited to their environment but often show low yields. Thus, in Niger, the flock of Koundoum sheep are rapidly decreasing. The Koundoum is one of the few wool sheep breeds of Africa and shows important adaptive feature to its native environment, i.e. the humid pastures on the banks of the Niger River. To characterise the breed and to understand its production context, a survey has been conducted in 104 herds in four communes along the Niger River (Kollo, Tillabery, Say and Tera). Nine body measurements, including live weight, were taken on 180 adult sheep (101 females and 79 males). The herds varied from 2 to 60 heads, with a median size of eight animals and two thirds of the herds having less than 10 animals. Mainly fed on natural pastures, 85.6% of the herds received crop residues. Only natural mating was practiced. Veterinary care was restricted to anti-helminthic and some indigenous treatments. The frequent affiliation of breeders to professional unions appeared as favourable to the implementation of a collective conservation program. The Koundoum sheep were white or black coated, with the black colour being most frequent (75.6%). Wattles were present in both sexes at similar frequencies of around 14%. All biometric variables were significantly and positively correlated between them. The thoracic perimeter showed the best correlation with live weight in both males and females. Three variables were selected for live weight prediction: thoracic perimeter, height at withers and rump length. From the present study, it is expected that the in situ conservation of the Koundoum sheep will be highly problematic, due to lack of market opportunities for wool and the willingness of smallholders to get involved in pure Koundoum rearing. [less ▲]

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See detailACE inhibitors in aortic stenosis: no fear just hope.
DAVIN, Laurent ULg; DULGHERU, Raluca Elena ULg; Lancellotti, Patrizio ULg

in European heart journal cardiovascular Imaging (2015)

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See detailRole of multimodality cardiac imaging in the management of patients with hypertrophic cardiomyopathy: an expert consensus of the European Association of Cardiovascular Imaging Endorsed by the Saudi Heart Association.
Cardim, Nuno; Galderisi, Maurizio; Edvardsen, Thor et al

in European heart journal cardiovascular Imaging (2015)

Taking into account the complexity and limitations of clinical assessment in hypertrophic cardiomyopathy (HCM), imaging techniques play an essential role in the evaluation of patients with this disease ... [more ▼]

Taking into account the complexity and limitations of clinical assessment in hypertrophic cardiomyopathy (HCM), imaging techniques play an essential role in the evaluation of patients with this disease. Thus, in HCM patients, imaging provides solutions for most clinical needs, from diagnosis to prognosis and risk stratification, from anatomical and functional assessment to ischaemia detection, from metabolic evaluation to monitoring of treatment modalities, from staging and clinical profiles to follow-up, and from family screening and preclinical diagnosis to differential diagnosis. Accordingly, a multimodality imaging (MMI) approach (including echocardiography, cardiac magnetic resonance, cardiac computed tomography, and cardiac nuclear imaging) is encouraged in the assessment of these patients. The choice of which technique to use should be based on a broad perspective and expert knowledge of what each technique has to offer, including its specific advantages and disadvantages. Experts in different imaging techniques should collaborate and the different methods should be seen as complementary, not as competitors. Each test must be selected in an integrated and rational way in order to provide clear answers to specific clinical questions and problems, trying to avoid redundant and duplicated information, taking into account its availability, benefits, risks, and cost. [less ▲]

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See detailA Systematic Review of Cost-Effectiveness Analyses of Drugs for Postmenopausal Osteoporosis.
Hiligsmann, Mickaël ULg; Evers, Silvia M.; Ben Sedrine, Wafa et al

in PharmacoEconomics (2015), 33(3), 205-224

BACKGROUND: Given the limited availability of healthcare resources and the recent introduction of new anti-osteoporosis drugs, the interest in the cost effectiveness of drugs in postmenopausal ... [more ▼]

BACKGROUND: Given the limited availability of healthcare resources and the recent introduction of new anti-osteoporosis drugs, the interest in the cost effectiveness of drugs in postmenopausal osteoporosis remains and even increases. OBJECTIVE: This study aims to identify all recent economic evaluations on drugs for postmenopausal osteoporosis, to critically appraise the reporting quality, and to summarize the results. METHODS: A literature search using Medline, the National Health Service Economic Evaluation database and the Cost-Effectiveness Analysis Registry was undertaken to identify original articles published between January 1, 2008 and December 31, 2013. Studies that assessed cost effectiveness of drugs in postmenopausal osteoporosis were included. The Consolidated Health Economic Evaluation Reporting Standards (CHEERS) statement was used to assess the quality of reporting of these articles. RESULTS: Of 1,794 articles identified, 39 studies fulfilled the inclusion criteria. They were conducted in 14 different countries and nine active interventions were assessed. When compared with no treatment, active osteoporotic drugs were generally cost effective in postmenopausal women aged over 60-65 years with low bone mass, especially those with prior vertebral fractures. Key drivers of cost effectiveness included individual fracture risk, medication adherence, selected comparators and country-specific analyses. Quality of reporting varied between studies with an average score of 17.9 out of 24 (range 7-21.5). CONCLUSION: This review found a substantial number of published cost-effectiveness analyses of drugs in osteoporosis in the last 6 years. Results and critical appraisal of these articles can help decision makers when prioritizing health interventions and can inform the development of future economic evaluations. [less ▲]

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See detailTrebananib (AMG 386) plus weekly paclitaxel with or without bevacizumab as first-line therapy for HER2-negative locally recurrent or metastatic breast cancer: A phase 2 randomized study.
Dieras, Veronique; Wildiers, Hans; Jassem, Jacek et al

in Breast (Edinburgh, Scotland) (2015), 24

INTRODUCTION: This phase 2 randomized study evaluated trebananib (AMG 386), a peptide-Fc fusion protein that inhibits angiogenesis by neutralizing the interaction of angiopoietin-1 and -2 with Tie2, in ... [more ▼]

INTRODUCTION: This phase 2 randomized study evaluated trebananib (AMG 386), a peptide-Fc fusion protein that inhibits angiogenesis by neutralizing the interaction of angiopoietin-1 and -2 with Tie2, in combination with paclitaxel with or without bevacizumab in previously untreated patients with HER2-negative locally recurrent/metastatic breast cancer. METHODS: Patients received paclitaxel 90 mg/m2 once weekly (3-weeks-on/1-week-off) and were randomly assigned 1:1:1:1 to also receive blinded bevacizumab 10 mg/kg once every 2 weeks plus either trebananib 10 mg/kg once weekly (Arm A) or 3 mg/kg once weekly (Arm B), or placebo (Arm C); or open-label trebananib 10 mg/kg once a week (Arm D). Progression-free survival was the primary endpoint. RESULTS: In total, 228 patients were randomized. Median estimated progression-free survival for Arms A, B, C, and D was 11.3, 9.2, 12.2, and 10 months, respectively. Hazard ratios (95% CI) for Arms A, B, and D versus Arm C were 0.98 (0.61-1.59), 1.12 (0.70-1.80), and 1.28 (0.79-2.09), respectively. The objective response rate was 71% in Arm A, 51% in Arm B, 60% in Arm C, and 46% in Arm D. The incidence of grade 3/4/5 adverse events was 71/9/4%, 61/14/5%, 62/16/3%, and 52/4/7% in Arms A/B/C/D. In Arm D, median progression-free survival was 12.8 and 7.4 months for those with high and low trebananib exposure (AUCss >/= 8.4 versus < 8.4 mg.h/mL), respectively. CONCLUSIONS: There was no apparent prolongation of estimated progression-free survival with the addition of trebananib to paclitaxel and bevacizumab at the doses tested. Toxicity was manageable. Exposure-response analyses support evaluation of combinations incorporating trebananib at doses > 10 mg/kg in this setting. TRIAL REGISTRATION: ClinicalTrials.gov, NCT00511459. [less ▲]

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See detailShort-term Effect of Infliximab Is Reflected in the Clot Lysis Profile of Patients with Inflammatory Bowel Disease: A Prospective Study.
Bollen, Lize; Vande Casteele, Niels; Peeters, Miet et al

in Inflammatory bowel diseases (2015), 21(3), 570-8

BACKGROUND: Inflammatory bowel disease (IBD) is recognized as an independent risk factor for thrombosis. First, we investigate whether the concentration of fibrinolysis inhibitors is increased in patients ... [more ▼]

BACKGROUND: Inflammatory bowel disease (IBD) is recognized as an independent risk factor for thrombosis. First, we investigate whether the concentration of fibrinolysis inhibitors is increased in patients with IBD. Second, we investigate the effect of infliximab induction therapy on the hemostatic profile. METHODS: This prospective study included 103 patients with IBD starting infliximab therapy and 113 healthy controls. Plasma was collected before the first infliximab infusion (wk 0) and after induction therapy (wk 14). Patients not showing a clinical response on induction were considered as primary nonresponders. Fibrinolysis inhibitors were measured by enzyme-linked immunosorbent assay. Using a clot lysis assay, the area under the curve (global marker for coagulation/fibrinolysis), 50% clot lysis time (marker for fibrinolytic capacity), and amplitude (indicator for clot formation) were determined. RESULTS: Patients with IBD selected for infliximab treatment have higher area under the curve (median 29 [interquartile range, 20-38]) and amplitude (0.4 [0.3-0.5]) compared with healthy controls (18 [13-24] and 0.3 [0.2-0.3], respectively, P < 0.001). Primary nonresponders showed a decrease neither in inflammatory markers nor in hemostatic parameters, whereas in primary responders, a decrease in inflammatory markers was associated with a decrease in both area under the curve (29 [20-38] (wk 0) to 20 [14-28] (wk 14), P < 0.001) and amplitude (0.4 [0.3-0.5] (wk 0) to 0.3 [0.3-0.4] (wk 14), P < 0.001). CONCLUSIONS: This is the first prospective study demonstrating that the clot lysis profile differs between patients with IBD and healthy individuals. On infliximab induction treatment, this clot lysis profile normalizes in responders suggesting that infliximab treatment is advisable for patients with IBD with an activated hemostatic profile. [less ▲]

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See detailThe prediction of fund failure through performance diagnostics
Cogneau, Philippe ULg; Hübner, Georges ULg

in Journal of Banking and Finance (2015), 50

Using an international database featuring 1624 mutual funds over 15 years, this paper analyses the joint abilities of performance measures to predict subsequent fund failure. We examine the probability of ... [more ▼]

Using an international database featuring 1624 mutual funds over 15 years, this paper analyses the joint abilities of performance measures to predict subsequent fund failure. We examine the probability of disappearance over a time window, and expected fund survival time, and study the circumstances of a fund’s disappearance, its currency and domicile. By combining relevant measures, fund failure appears to a significant extent predictable, more than with single classical measures. Survivorship predictability has significant economic value. Such evidence suggests that past performance does not only influence investors’ perception of fund quality, but also reflects managers’ ability to sustain performance. [less ▲]

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See detailBoosting regulatory T-cell function with the humanized CD4-specific humanized monoclonal antibody Tregalizumab (BT-061)
Humblet-Baron, Stéphanie; Baron, Frédéric ULg

in Immunology & Cell Biology (2015), 1-2

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See detailBayesian inversions of a dynamic vegetation model at four European grassland sites
Minet, Julien ULg; Laloy, Eric; Tychon, Bernard ULg et al

in Biogeosciences (2015), 12(9), 2809--2829

Eddy covariance data from four European grassland sites are used to probabilistically invert the CARAIB (CARbon Assimilation In the Biosphere) dynamic vegetation model (DVM) with 10 unknown parameters ... [more ▼]

Eddy covariance data from four European grassland sites are used to probabilistically invert the CARAIB (CARbon Assimilation In the Biosphere) dynamic vegetation model (DVM) with 10 unknown parameters, using the DREAM(ZS) (DiffeRential Evolution Adaptive Metropolis) Markov chain Monte Carlo (MCMC) sampler. We focus on comparing model inversions, considering both homoscedastic and heteroscedastic eddy covariance residual errors, with variances either fixed a priori or jointly inferred together with the model parameters. Agreements between measured and simulated data during calibration are comparable with previous studies, with root mean square errors (RMSEs) of simulated daily gross primary productivity (GPP), ecosystem respiration (RECO) and evapotranspiration (ET) ranging from 1.73 to 2.19, 1.04 to 1.56 g C m−2 day−1 and 0.50 to 1.28 mm day−1, respectively. For the calibration period, using a homoscedastic eddy covariance residual error model resulted in a better agreement between measured and modelled data than using a heteroscedastic residual error model. However, a model validation experiment showed that CARAIB models calibrated considering heteroscedastic residual errors perform better. Posterior parameter distributions derived from using a heteroscedastic model of the residuals thus appear to be more robust. This is the case even though the classical linear heteroscedastic error model assumed herein did not fully remove heteroscedasticity of the GPP residuals. Despite the fact that the calibrated model is generally capable of fitting the data within measurement errors, systematic bias in the model simulations are observed. These are likely due to model inadequacies such as shortcomings in the photosynthesis modelling. Besides the residual error treatment, differences between model parameter posterior distributions among the four grassland sites are also investigated. It is shown that the marginal distributions of the specific leaf area and characteristic mortality time parameters can be explained by site-specific ecophysiological characteristics. [less ▲]

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See detailDairy farms typology and management of animal genetic resources in the peri-urban zone of Bamako (Mali)
Toure, Abdoulaye; Moula, Nassim ULg; Kouriba, Ali et al

in Journal of Agriculture and Rural Development in the Tropics and Subtropics (2015), 116(1), 37-47

Facing growth in demand, dairy production in peri-urban areas of developing countries is changing rapidly. To characterise this development around Bamako (Mali), this study establishes a typology of dairy ... [more ▼]

Facing growth in demand, dairy production in peri-urban areas of developing countries is changing rapidly. To characterise this development around Bamako (Mali), this study establishes a typology of dairy production systems with a special focus on animal genetic resources. The survey included 52 dairy cattle farms from six peri-urban sites. It was conducted in 2011 through two visits, in the dry and harvest seasons. The median cattle number per farm was 17 (range 5–118) and 42% of farmers owned cropland (8.3± 7.3 ha, minimum 1 ha, maximum 25 ha). Feeding strategy was a crucial variable in farm characterisation, accounting for about 85% of total expenses. The use of artificial insemination and a regular veterinary follow-up were other important parameters. According to breeders’ answers, thirty genetic profiles were identified, from local purebreds to different levels of crossbreds. Purebred animals raised were Fulani Zebu (45.8 %), Maure Zebu (9.2 %), Holstein (3.0 %), Azawak Zebu (1.3 %), Mere Zebu (0.5%) and Kuri taurine (0.1 %). Holstein crossbred represented 30.5% of the total number of animals (19.0% Fulani-Holstein, 11.2% Maure-Holstein and 0.3% Kuri-Holstein). Montbéliarde, Normande and Limousin crossbreds were also found (6.6 %, 0.7% and 0.3 %, respectively). A multivariate analysis helped disaggregate the diversity of management practices. The high diversity of situations shows the need for consideration of typological characteristics for an appropriate intervention. Although strongly anchored on local breeds, the peri-urban dairy systems included a diversity of exotic cattle, showing an uncoordinated quest of breeders for innovation. Without a public intervention, this dynamic will result in an irremediable erosion of indigenous animal genetic resources. [less ▲]

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See detailA Stereocontrolled Synthesis of the Hydrophobic Moiety of Rhamnolipids
Menhour, Boudjema; Mayon, Patrick; Plé, Karen et al

in Tetrahedron Letters (2015), 56

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See detailStellar acoustic radii, mean densities, and ages from seismic inversion techniques
Buldgen, Gaël ULg; Dupret, Marc-Antoine ULg; Reese, Daniel et al

in Astronomy and Astrophysics (2015), 574

Context. Determining stellar characteristics such as the radius, mass or age is crucial when studying stellar evolution or exoplanetary systems, or when characterising stellar populations in the Galaxy ... [more ▼]

Context. Determining stellar characteristics such as the radius, mass or age is crucial when studying stellar evolution or exoplanetary systems, or when characterising stellar populations in the Galaxy. Asteroseismology is the golden path to accurately obtain these characteristics. In this context, a key question is how to make these methods less model-dependent. Aims: Building on the previous work of Daniel Reese, we wish to extend the Substractive Optimally Localized Averages (SOLA) inversion technique to new stellar global characteristics beyond the mean density. The goal is to provide a general framework in which to estimate these characteristics as accurately as possible in low-mass main-sequence stars. Methods: First, we describe our framework and discuss the reliability of the inversion technique and possible sources of error. We then apply this methodology to the acoustic radius, an age indicator based on the sound speed derivative and the mean density, and compare it to estimates based on the average large and small frequency separations. These inversions are carried out for several test cases including various metallicities, different mixing-lengths, non-adiabatic effects, and turbulent pressure. Results: We observe that the SOLA method yields accurate results in all test cases whereas results based on the large and small frequency separations are less accurate and more sensitive to surface effects and structural differences in the models. If we include the surface corrections of Kjeldsen et al. (2008, ApJ, 683, L175), we obtain results of comparable accuracy for the mean density. Overall, the mean density and acoustic radius inversions are more robust than the inversions for the age indicator. Moreover, the current approach is limited to relatively young stars with radiative cores. Increasing the number of observed frequencies improves the reliability and accuracy of the method. [less ▲]

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See detailIntegrating hydrological features and 1 genetically validated occurrence data in occupancy 2 modeling of an endemic and endangered semi‐aquatic mammal species, Galemys pyrenaicus. 3 4
Charbonnel, Anaïs; Buisson, Laetitia; Biffi, Marjorie et al

in Biological Conservation (2015), Sous Presse

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See detailJean-Marie KAJDANSKI, Pain gris, 2014.
Baiwir, Esther ULg

in Wallonnes : Chronique de la Société de Langue et de Littérature Wallonnes (2015), (1/2015), 13-14

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See detailL'infraction d'urbanisme qui affecte l'immeuble vendu donne-t-elle lieu à la garantie d'éviction ou à la garantie des vices cachés?
Onclin, François ULg

in Revue Générale de Droit Civil Belge = Tijdschrift voor Belgisch Burgerlijk (2015), (1), 24-34

Cette étude commente un arrêt de la Cour d'appel de Liège prononcé le 10 mai 2012. Quelles sont les voies d'action qui s'ouvrent à l'acheteur insatisfait qui découvre, après la vente, que le bien immeuble ... [more ▼]

Cette étude commente un arrêt de la Cour d'appel de Liège prononcé le 10 mai 2012. Quelles sont les voies d'action qui s'ouvrent à l'acheteur insatisfait qui découvre, après la vente, que le bien immeuble qu'il vient d'acquérir est en situation infractionnelle sur le plan du droit de l'urbanisme ? La question se pose de savoir si l'infraction d'urbanisme constitue une cause d'éviction ou un vice caché au sens du Code civil. [less ▲]

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See detailFilter-free exhaustive odds ratio-based genome-wide interaction approach pinpoints evidence for interaction in the HLA region in psoriasis.
Grange, Laura; Bureau, Jean-Francois; Nikolayeva, Iryna et al

in BMC genetics (2015), 16(1), 11

BackgroundDeciphering the genetic architecture of complex traits is still a major challenge for human genetics. In most cases, genome-wide association studies have only partially explained the ... [more ▼]

BackgroundDeciphering the genetic architecture of complex traits is still a major challenge for human genetics. In most cases, genome-wide association studies have only partially explained the heritability of traits and diseases. Epistasis, one potentially important cause of this missing heritability, is difficult to explore at the genome-wide level. Here, we develop and assess a tool based on interactive odds ratios (IOR), Fast Odds Ratio-based sCan for Epistasis (FORCE), as a novel approach for exhaustive genome-wide epistasis search. IOR is the ratio between the multiplicative term of the odds ratio (OR) of having each variant over the OR of having both of them. By definition, an IOR that significantly deviates from 1 suggests the occurrence of an interaction (epistasis). As the IOR is fast to calculate, we used the IOR to rank and select pairs of interacting polymorphisms for P value estimation, which is more time consuming.ResultsFORCE displayed power and accuracy similar to existing parametric and non-parametric methods, and is fast enough to complete a filter-free genome-wide epistasis search in a few days on a standard computer. Analysis of psoriasis data uncovered novel epistatic interactions in the HLA region, corroborating the known major and complex role of the HLA region in psoriasis susceptibility.ConclusionsOur systematic study revealed the ability of FORCE to uncover novel interactions, highlighted the importance of exhaustiveness, as well as its specificity for certain types of interactions that were not detected by existing approaches. We therefore believe that FORCE is a valuable new tool for decoding the genetic basis of complex diseases. [less ▲]

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See detailComplex dynamics of a nonlinear aerospace structure: numerical continuation and normal modes
Renson, Ludovic ULg; Noël, Jean-Philippe ULg; Kerschen, Gaëtan ULg

in Nonlinear Dynamics (2015), 79(2), 1293-1309

This paper investigates the dynamics of a real-life aerospace structure possessing a strongly nonlinear component with multiple mechanical stops. A full-scale finite element model is built for gaining ... [more ▼]

This paper investigates the dynamics of a real-life aerospace structure possessing a strongly nonlinear component with multiple mechanical stops. A full-scale finite element model is built for gaining additional insight into the nonlinear dynamics that was observed experimentally, but also for uncovering additional nonlinear phenomena, such as quasiperiodic regimes of motion. Forced/unforced, damped/undamped numerical simulations are carried out using advanced techniques and theoretical concepts such as numerical continuation and nonlinear normal modes. [less ▲]

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See detailCombined estrogenic and anti-estrogenic properties of estetrol on breast cancer may provide a safe therapeutic window for the treatment of menopausal symptoms
Gérard, Céline ULg; Mestdagt, Mélanie; Tskitishvili, Ekaterine ULg et al

in Oncotarget (2015)

Increased risk of breast cancer is a critical side effect associated with the use of a menopausal hormone therapy (MHT). Estetrol (E4) is a natural estrogen produced by the human fetal liver and is a ... [more ▼]

Increased risk of breast cancer is a critical side effect associated with the use of a menopausal hormone therapy (MHT). Estetrol (E4) is a natural estrogen produced by the human fetal liver and is a promising compound for clinical use in MHT. However, its impact on breast cancer is controversial and poorly defined. In this preclinical study, we show that E4 acts as a weak estrogen by stimulating the growth of hormone-dependent breast cancer only at concentrations exceeding menopausal therapeutic needs. E4 presents also an antitumor activity by decreasing the strong proliferative effect of estradiol (E2). While estrogen receptor alpha (ERα) is the predominant receptor mediating its effects, the dual weak-estrogenic/anti-estrogenic feature of E4 results from differential signaling pathways activation. Both nuclear and rapid extra-nuclear signaling pathway are necessary for a complete estrogenic effect of E4. However, the antitumor action of E4 is not due to a capacity to antagonize E2-induced nuclear activity. Altogether, our results highlight that E4 has a limited impact on breast cancer and may offer a safe therapeutic window for the treatment of menopausal symptoms. [less ▲]

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See detailMerging multi-camera data to reduce motion analysis instrumental errors using Kalman filters
Schwartz, Cédric ULg; Denoël, Vincent ULg; Forthomme, Bénédicte ULg et al

in Computer Methods in Biomechanics and Biomedical Engineering (2015), 18(9), 952-960

In motion capture systems, markers are often seen by multiple cameras. All cameras do not measure the position of the markers with the same reliability because of environmental factors such as the ... [more ▼]

In motion capture systems, markers are often seen by multiple cameras. All cameras do not measure the position of the markers with the same reliability because of environmental factors such as the position of the marker in the field of view or the light intensity received by the cameras. Kalman filters offer a general framework to take the reliability of the various cameras into account and consequently improve the estimation of the marker position. The proposed process can be applied to both passive and active systems. Several reliability models of the cameras are compared for the Codamotion active system, which is considered as a specific illustration. The proposed method significantly reduces the noise in the signal, especially at long range distances. Therefore, it improves the confidence of the positions at the limits of the field of view. [less ▲]

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See detailOne-year outcomes in 1,010 unselected patients treated with the PROMUS Element everolimus-eluting stent: the multicentre PROMUS Element European Post-Approval Surveillance Study
Thomas, MR; Birkemeyer, R; Schwimmbeck, P et al

in EuroIntervention : Journal of EuroPCR in Collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology (2015), 10

Aims: The PROMUS™ Element™ European Post-Approval Surveillance Study (PE-Prove) is a prospective, open-label, multicentre observational study designed to assess outcomes following PROMUS Element ... [more ▼]

Aims: The PROMUS™ Element™ European Post-Approval Surveillance Study (PE-Prove) is a prospective, open-label, multicentre observational study designed to assess outcomes following PROMUS Element everolimus-eluting stent implantation in an unselected patient population. Methods and results: A total of 1,010 patients were enrolled at 40 clinical sites in Europe, including 24.9% with medically treated diabetes, 50.0% with Type B2/C lesions, 6.1% with chronic total occlusion, 17.8% with acute myocardial infarction (MI ≤24 hours pre-procedure), and 20.1% with unstable angina. The target lesion was the culprit for ST-segment elevation MI in 7.3% of patients. The one-year, per patient target vessel failure rate was 6.2% (60/975), 3.4% (33) being related to the PROMUS Element stent. Rates of cardiac death, MI, and Academic Research Consortium (ARC) definite/probable stent thrombosis were 1.7%, 3.5%, and 0.6%, respectively. The target vessel revascularisation rate was 3.2% (31/975), 2.1% (20) being related to the PROMUS Element stent. Conclusions: In a large and relatively complex group of “real-world” patients, coronary artery revascularisation with the PROMUS Element everolimus-eluting stent provides favourable results with low event rates consistent with those reported for other contemporary drug-eluting stents. [less ▲]

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See detailAbsence of female-typical pheromone-induced hypothalamic neural responses and kisspeptin neuronal activity in alpha-fetoprotein knockout female mice.
Taziaux, Mélanie ULg; Bakker, Julie ULg

in Endocrinology (2015)

Pheromones induce sexually dimorphic neuroendocrine responses, such as LH secretion. However, the neuronal network by which pheromones are converted into signals that will initiate and modulate endocrine ... [more ▼]

Pheromones induce sexually dimorphic neuroendocrine responses, such as LH secretion. However, the neuronal network by which pheromones are converted into signals that will initiate and modulate endocrine changes remains unclear. We asked whether two sexually dimorphic populations in the anteroventral periventricular and periventricular nuclei (AVPV/PeN) that express kisspeptin and tyrosine hydroxylase (TH) are potential candidates that will transduce the olfactory signal to the neuroendocrine system. Furthermore, we assessed whether this transduction is sensitive to perinatal actions of estradiol by using female mice deficient in alpha-fetoprotein (AfpKO), which lack the protective actions of Afp against maternal estradiol. Wild-type (WT) and AfpKO male and female mice were exposed to same- versus opposite-sex odors and the expression of c-Fos was analyzed along the olfactory projection pathways as well as whether kisspeptin, TH and gonadotropin-releasing hormone (GnRH) neurons are responsive to opposite-sex odors. Male odors induced a female-typical Fos expression in target forebrain sites of olfactory inputs involved in reproduction in WT, but not in AfpKO females, whereas female odors induced a male-typical Fos expression in males of both genotypes. In WT females, opposite-sex odors induced Fos in kisspeptin and TH neurons, whereas in AfpKO females and WT males, only a lower, but still significant, Fos expression was observed in TH, but not in kisspeptin neurons. Finally, opposite-sex odors did not induce any significant Fos expression in GnRH neurons of both sexes or genotypes. Our results strongly suggest a role for fetal estrogen in the sexual differentiation of neural responses to sex-related olfactory cues. [less ▲]

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See detailPostcolonial Thesholds: Gateways and Borders
Wilson, Janet; Tunca, Daria ULg

in Journal of Postcolonial Writing (2015), 51(1), 1-107

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See detailPharmacodynamics, Efficacy and Safety of Sodium-Glucose Co-Transporter Type 2 (SGLT2) Inhibitors for the Treatment of Type 2 Diabetes Mellitus.
Scheen, André ULg

in Drugs (2015), 75(1), 33-59

Inhibitors of sodium-glucose co-transporter type 2 (SGLT2) are proposed as a novel approach for the management of type 2 diabetes mellitus (T2DM). Several compounds are already available in many countries ... [more ▼]

Inhibitors of sodium-glucose co-transporter type 2 (SGLT2) are proposed as a novel approach for the management of type 2 diabetes mellitus (T2DM). Several compounds are already available in many countries (dapagliflozin, canagliflozin, empagliflozin and ipragliflozin) and some others are in a late phase of development. The available SGLT2 inhibitors share similar pharmacokinetic characteristics, with a rapid oral absorption, a long elimination half-life allowing once-daily administration, an extensive hepatic metabolism mainly via glucuronidation to inactive metabolites, the absence of clinically relevant drug-drug interactions and a low renal elimination as parent drug. SGLT2 co-transporters are responsible for reabsorption of most (90 %) of the glucose filtered by the kidneys. The pharmacological inhibition of SGLT2 co-transporters reduces hyperglycaemia by decreasing renal glucose threshold and thereby increasing urinary glucose excretion. The amount of glucose excreted in the urine depends on both the level of hyperglycaemia and the glomerular filtration rate. Results of numerous placebo-controlled randomised clinical trials of 12-104 weeks duration have shown significant reductions in glycated haemoglobin (HbA1c), resulting in a significant increase in the proportion of patients reaching HbA1c targets, and a significant lowering of fasting plasma glucose when SGLT2 inhibitors were administered as monotherapy or in addition to other glucose-lowering therapies including insulin in patients with T2DM. In head-to-head trials of up to 2 years, SGLT2 inhibitors exerted similar glucose-lowering activity to metformin, sulphonylureas or sitagliptin. The durability of the glucose-lowering effect of SGLT2 inhibitors appears to be better; however, this remains to be more extensively investigated. The risk of hypoglycaemia was much lower with SGLT2 inhibitors than with sulphonylureas and was similarly low as that reported with metformin, pioglitazone or sitagliptin. Increased renal glucose elimination also assists weight loss and could help to reduce blood pressure. Both effects were very consistent across the trials and they represent some advantages for SGLT2 inhibitors when compared with other oral glucose-lowering agents. The pharmacodynamic response to SGLT2 inhibitors declines with increasing severity of renal impairment, and prescribing information for each SGLT2 inhibitor should be consulted regarding dosage adjustments or restrictions in moderate to severe renal dysfunction. Caution is also recommended in the elderly population because of a higher risk of renal impairment, orthostatic hypotension and dehydration, even if the absence of hypoglycaemia represents an obvious advantage in this population. The overall effect of SGLT2 inhibitors on the risk of cardiovascular disease is unknown and will be evaluated in several ongoing prospective placebo-controlled trials with cardiovascular outcomes. The impact of SGLT2 inhibitors on renal function and their potential to influence the course of diabetic nephropathy also deserve more attention. SGLT2 inhibitors are generally well-tolerated. The most frequently reported adverse events are female genital mycotic infections, while urinary tract infections are less commonly observed and generally benign. In conclusion, with their unique mechanism of action that is independent of insulin secretion and action, SGLT2 inhibitors are a useful addition to the therapeutic options available for the management of T2DM at any stage in the natural history of the disease. Although SGLT2 inhibitors have already been extensively investigated, further studies should even better delineate the best place of these new glucose-lowering agents in the already rich armamentarium for the management of T2DM. [less ▲]

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See detailL’acte incendiaire, son sujet et sa signification : propositions à partir du Saint Genet de Jean-Paul Sartre
Englebert, Jérôme ULg

in Annales Médico-Psychologiques (2015), 172(2), 186-192

Objectives In this paper we studied fire setters with the hermeneutics’ method from the philosophy of Jean-Paul Sartre. In the psychobiography of Jean Genet, the philosopher raises issues of the ... [more ▼]

Objectives In this paper we studied fire setters with the hermeneutics’ method from the philosophy of Jean-Paul Sartre. In the psychobiography of Jean Genet, the philosopher raises issues of the infractional acting and its social meaning. Methods After recalling the basic notions of international literature about fire setters (including confusion with pyromania, diagnostic problems proposed by the DSM IV and the concept of "communicative arson"), we propose phenomenological and existential clues to discuss the act and its meaning. It is the unlawful act that is analysed and more specifically the arson. We propose different analysis than the psychic causality which is inspired by psychoanalysis. The criminogenesis gives way to a questioning of identity, about the place of fire in the subject’s history and within its relationships to others. As proposed by Sartre, the paradigm of this research is to consider the meaning "as the return of the future in the present". The second field of this study is an analysis of three clinical cases. These cases are the result of a forensic practice. The method is focused on the analysis of a narrative and autobiographical production. By this way we studied the temporal process of these three arsonists. Results A comparative reading of the psychobiography of Jean Genet written by Sartre and of the situation of arsonists gives accurate information. The "criminogenesis" gives way to a questioning of identity. In this way we discuss the meaning of fire in the subject's history and within its relationship to others. We highlight the inscription of the act in the social and constitutive process of the individual. From the clinical material, the author demonstrates a link between arson and construction of identity. Identity is considered as a dialectic between “ipse” and “idem”, as suggested by Ricœur. We can therefore consider the real sense that the arson has from the perspective of the actor but also from the viewpoint of the society and these conceptions may be similar or different. In clinical practice, we recommend to pay special attention to how the fire is part of the biography of the patient. Conclusions The fascination for fire is a source of meaning which can be radically different depending on whether we consider the external interpreter (the world or the clinician) or the author of the action. The basic principles of hermeneutics, as described by Gadamer and Ricœur, are to demonstrate that the understanding and the interpretation are not only the methods used in the human sciences but are also part of the fundamental processes inherent in human nature. Hermeneutics in general is the opposite of a doctrine of truth. It rather seeks to show arbitrarily how the interpretation and the search for meaning are paradigmatic of the human experience. [less ▲]

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See detailReversing song behavior phenotype: Testosterone driven induction of singing and measures of song quality in adult male and female canaries (Serinus canaria)
Madison, Farrah N.; Rouse, Melvin L. Jr; Balthazart, Jacques ULg et al

in General and comparative endocrinology (2015), 215

In songbirds, such as canaries (Serinus canaria), the song control circuit has been shown to undergo a remarkable change in morphology in response to exogenous testosterone (T). It is also well ... [more ▼]

In songbirds, such as canaries (Serinus canaria), the song control circuit has been shown to undergo a remarkable change in morphology in response to exogenous testosterone (T). It is also well established that HVC, a telencephalic nucleus involved in song production, is significantly larger in males than in females. T regulates seasonal changes in HVC volume in males, and exposure to exogenous T in adult females increases HVC volume and singing activity such that their song becomes more male-like in frequency and structure. However, whether there are sex differences in the ability of T to modulate changes in the song system and song behavior has not been investigated in canaries. In this study, we compared the effects of increasing doses of T on singing and song control nuclei volumes in adult male and female American Singer canaries exposed to identical environmental conditions. Males were castrated and all birds were placed on short days (8L:16D) for 8weeks. Males and females were implanted either with a 2, 6 or 12mm long Silastic implant filled with crystalline T or an empty 12mm implant as control. Birds were then housed individually in sound-attenuated chambers. Brains were collected from six birds from each group after 1week or 3weeks of treatment. Testosterone was not equally effective in increasing singing activity in both males and females. Changes in song quality and occurrence rate took place after a shorter latency in males than in females; however, females did undergo marked changes in a number of measures of song behavior if given sufficient time. Males responded with an increase in HVC volume at all three doses. In females, T-induced changes in HVC volume only had limited amplitude and these volumes never reached male-typical levels, suggesting that there are sex differences in the neural substrate that responds to T. [less ▲]

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See detailImpact of 6 % hydroxyethyl starch (HES) 130/0.4 on the correlation between standard laboratory tests and thromboelastography (TEG(R)) after cardiopulmonary bypass.
HANS, Grégory ULg; Hartstein, Gary ULg; Roediger, Laurence et al

in Thrombosis research (2015), 135(5), 984-9

BACKGROUND: Hydroxyethyl starches (HES) affect the results of thromboelastography (TEG(R)). We sought to determine whether using HES rather than crystalloids for cardiopulmonary bypass (CPB) prime and ... [more ▼]

BACKGROUND: Hydroxyethyl starches (HES) affect the results of thromboelastography (TEG(R)). We sought to determine whether using HES rather than crystalloids for cardiopulmonary bypass (CPB) prime and intraoperative fluid therapy changes the TEG cutoff values best identifying patients with a low platelet count or a low fibrinogen level after CPB. METHODS: Data from 96 patients who had on-pump cardiac surgery, a TEG(R) (kaolin-heparinase) and standard investigations of blood clotting performed after separation from CPB and protamine administration were retrospectively reviewed. Patients were assigned to the HES or crystalloid group according to whether balanced 6% HES 130/0.4 or balanced crystalloids were used for intraoperative fluid therapy and pump prime. Mutlivariable linear regression models with computation of the standardized regression coefficients were used to identify independent associations between the four main TEG parameters (R time, alpha angle, K time and MA) and the type of fluid used, the INR, the aPTT, the fibrinogen level and the platelet count. Receiver-operating-characteristic curves were used to assess the effect of HES on the ability of TEG parameters to identify patients with a platelet count<80.000mul(-1) or a fibrinogen level<1.5 gr l(-1) and on the cutoff values best identifying these patients. RESULTS: The type of fluid used significantly affected the MA (P<0.001), the K time (P<0.001) and the alpha angle (P<0.001) regardless of the results of the standard clotting tests. According to standardized ss regression coefficients the platelet count and the type of fluid used were stronger predictors of the MA, the alpha angle and the K time than the fibrinogen level. MA better predicted platelets<80.000mul(-1) than K time and alpha angle (P=0.023). The best cutoff value of MA identifying patients with platelets<80.000mul(-1) was 62mm in the crystalloid group and 53mm in the HES group. MA, K time and alpha angle were poor predictors of the postoperative fibrinogen level. CONCLUSION: HES significantly changes the cutoff value of TEG(R) MA best identifying patients<80.000mul(-1) after on-pump cardiac surgery. [less ▲]

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See detailCorrelation between ethanol behavioral sensitization and midbrain dopamine neuron reactivity to ethanol
Didone, Vincent ULg; Masson, Sébastien; Quoilin, Caroline et al

in Addiction Biology (2015)

Repeated ethanol injections lead to a sensitization of its stimulant effects in mice. Some recent results argue against a role for ventral tegmental area (VTA) dopamine neurons in ethanol behavioral ... [more ▼]

Repeated ethanol injections lead to a sensitization of its stimulant effects in mice. Some recent results argue against a role for ventral tegmental area (VTA) dopamine neurons in ethanol behavioral sensitization. The aim of the present study was to test whether in vivo ethanol locomotor sensitization correlates with changes in either basal- or ethanol evoked firing rates of dopamine neurons in vitro. Female Swiss mice were daily injected with 2.5 g/kg ethanol (or saline in the control group) for 7 days and their locomotor activity was recorded. At the end of the sensitization procedure, extracellular recordings were made from dopaminergic neurons in midbrain slices from these mice. Significantly higher spontaneous basal firing rates of dopamine neurons were recorded in ethanol-sensitized mice relative to control mice, but without correlations with the behavioral effects. The superfusion of sulpiride, a dopamine D2 antagonist, induced a stronger increase of dopamine neuron firing rates in ethanol-sensitized mice. This shows that the D2 feedback in dopamine neurons is preserved after chronic ethanol administration and argues against a reduced D2 feedback as an explanation for the increased dopamine neuron basal firing rates in ethanol-sensitized mice. Finally, ethanol superfusion (10–100 mM) significantly increased the firing rates of dopamine neurons and this effect was of higher magnitude in ethanol-sensitized mice. Furthermore, there were significant correlations between such a sensitization of dopamine neuron activity and ethanol behavioral sensitization. These results support the hypothesis that changes in brain dopamine neuron activity contribute to the behavioral sensitization of the stimulant effects of ethanol. [less ▲]

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See detailInvestigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009
Beckers, L.; Masset, J.; Hamilton, C. et al

in Biochemical Engineering Journal (2015), 98

Fermentative hydrogen production has often been described as inhibited by its own gas production. In this work, hydrogen production by Clostridium butyricum was investigated in batch Biochemical Hydrogen ... [more ▼]

Fermentative hydrogen production has often been described as inhibited by its own gas production. In this work, hydrogen production by Clostridium butyricum was investigated in batch Biochemical Hydrogen Potential (BHP) tests and in a 2.5L anaerobic sequenced batch reactor (AnSBR) under different operating conditions regarding liquid-to-gas mass transfer. Through the addition of both stirring up to 400rpm and nitrogen sparging, the yields were enhanced from 1.6 to 3.1molH2molglucose -1 and the maximum hydrogen production rates from 140 to 278mLh-1. These original results were achieved with a pure Clostridium strain. They showed that hydrogen production was improved by a higher liquid-to-gas hydrogen transfer resulting in a lower dissolved hydrogen concentration in the culture medium and therefore in a lower bacterial inhibition. In addition, biohydrogen partitioning between the gas and the liquid phase did not conform to Henry's Law due to critical supersaturation phenomena up to seven-fold higher than the equilibrium conditions. Therefore, dissolved hydrogen concentration should be systematically measured instead of the headspace hydrogen partial pressure. A model was proposed to correlate H2 production yield and rate by the pure C. butyricum strain CWBI1009 with mass transfer coefficient KLa. [less ▲]

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See detailFramework for the integration of genomics, epigenomics, and transcriptomics in complex diseases
Pineda San Juan, Silvia ULg; Gómez-Rubio, Paulina; Antoni, Picornell et al

in Human Heredity (2015)

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See detailThe use of echocardiography in acute cardiovascular care: Recommendations of the European Association of Cardiovascular Imaging and the Acute Cardiovascular Care Association.
Lancellotti, Patrizio ULg; Price, Susanna; Edvardsen, Thor et al

in European Heart Journal. Acute Cardiovascular Care (2015)

Echocardiography is one of the most powerful diagnostic and monitoring tools available to the modern emergency/critical care practitioner. Currently, there is a lack of specific European Association of ... [more ▼]

Echocardiography is one of the most powerful diagnostic and monitoring tools available to the modern emergency/critical care practitioner. Currently, there is a lack of specific European Association of Cardiovascular Imaging/Acute Cardiovascular Care Association recommendations for the use of echocardiography in acute cardiovascular care. In this document, we describe the practical applications of echocardiography in patients with acute cardiac conditions, in particular with acute chest pain, acute heart failure, suspected cardiac tamponade, complications of myocardial infarction, acute valvular heart disease including endocarditis, acute disease of the ascending aorta and post-intervention complications. Specific issues regarding echocardiography in other acute cardiac care scenarios are also described. [less ▲]

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See detailTrophodynamics of estuarine intertidal harpacticoid copepods based on stable isotope composition and fatty acid profiles
Cnudde, Clio; Moens, Tom; Werbrouck, Eva et al

in Marine Ecology. Progress Series (2015), 524

Trophic interactions at the basis of food webs, for instance between meiofauna, primary producers and bacteria, are key drivers of benthic energy fluxes. Yet both qualitative and quantitative information ... [more ▼]

Trophic interactions at the basis of food webs, for instance between meiofauna, primary producers and bacteria, are key drivers of benthic energy fluxes. Yet both qualitative and quantitative information about meiofaunal resource utilization under in situ conditions is scant. By means of natural stable isotope ratios of carbon and nitrogen and of fatty acid (FA) profiles, we examined the variability of in situ resource utilization of a range of harpacticoid copepod species from 5 stations in an estuarine intertidal area. These stations, located in different habitats, differed in sediment granulometry, resource availability, presence/absence of vegetation and other environmental variables, as well as in copepod species composition. Our goal was to describe inter-specific differences among harpacticoid species, as well as spatio-temporal variability within species. Despite differences in resource availability between habitats, δ13C data clearly point at microphytobenthos (MPB) as the major carbon source to the harpacticoid assemblages at all 5 stations. Small differences in carbon isotopic ratios between co-occurring species indicate some degree of resource differentiation, whereas both the δ15N and FA composition suggest that several harpacticoid species obtain MPB carbon indirectly, perhaps through feeding on bacteria or ciliates. For a limited number of species, such as Paraleptastacus spinicauda, clear dietary contributions of suspended particulate matter and bacteria were found, and MPB appeared to have only a small or no contribution. Even in vegetated salt-marsh stations, Spartina anglica detritus did not appear to contribute to copepod diets. The δ13C of Cletodidae were highly depleted, reflecting a contribution of methane-derived carbon. [less ▲]

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See detailRelation between global end-diastolic volume and left ventricular end-diastolic volume
Pironet, Antoine ULg; MORIMONT, Philippe ULg; Kamoi, S. et al

in Critical Care (2015), 19(Suppl 1), 175

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See detailRestoring testosterone levels by adding dehydroepiandrosterone to a drospirenone containing combined oral contraceptive: I. Endocrine effects.
Zimmerman, Y.; Foidart, Jean-Michel ULg; Pintiaux, Axelle ULg et al

in Contraception (2015), 91(2), 127-33

OBJECTIVES: Combined oral contraceptives (COCs) decrease testosterone (T) levels. This study investigated restoration of T and other androgen concentrations during COC use by 'co-administration' of ... [more ▼]

OBJECTIVES: Combined oral contraceptives (COCs) decrease testosterone (T) levels. This study investigated restoration of T and other androgen concentrations during COC use by 'co-administration' of dehydroepiandrosterone (DHEA). STUDY DESIGN: In this randomized, double-blind, placebo-controlled study in 99 new COC starters (18-35 years old with body mass index range 18-34kg/m(2)), a COC containing 30mcg ethinylestradiol (EE) and 3mg drospirenone (DRSP) was used for 3cycles, followed by 6cycles of the same COC combined with either 50mg/day DHEA or placebo. Total T, albumin, sex hormone-binding globulin (SHBG), DHEA-sulfate (DHEA-S), Delta4-androstenedione (AD), 3alpha-androstanediol glucuronide (ADG) and estradiol (E2) were measured, whereas free T and the free T index (FTI) were calculated. Assessments took place at baseline (no COC use), after the run-in period (COC use alone) and during the treatment period (DHEA or placebo). RESULTS: During COC use alone, androgen levels decreased, especially total T by 62% and free T by 86%, and SHBG increased by 243%. Total T increased with DHEA compared to placebo (change from end of run-in period to end of treatment period - 1.3+/-1.2nmol/L vs. 0.0+/-0.4nmol/L; p<.0001) - and was restored to baseline levels. Free T and the FTI increased significantly (p<.0001), but the free T level was still 53% below baseline levels. DHEA-S, AD and ADG increased significantly to levels above baseline (p<.0001 for each). DHEA had no effect on SHBG, albumin and E2. CONCLUSIONS: An EE/DRSP containing COC strongly suppressed endogenous androgen concentrations in all users. The addition of 50mg DHEA to a COC regimen containing EE/DRSP restored total T to baseline levels, but free T levels were restored by only 47% as most of the T remains bound to SHBG. IMPLICATIONS: When using a COC that increases SHBG considerably, a daily dose of 50mg DHEA is insufficient to normalize free T levels completely. [less ▲]

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See detailLINKPHASE3: an improved pedigree-based phasing algorithm robust to genotyping and map errors.
Druet, Tom ULg; Georges, Michel ULg

in Bioinformatics (Oxford, England) (2015), 31(10), 1677-9

Many applications in genetics require haplotype reconstruction. We present a phasing program designed for large half-sibs families (as observed in plant and animals) that is robust to genotyping and map ... [more ▼]

Many applications in genetics require haplotype reconstruction. We present a phasing program designed for large half-sibs families (as observed in plant and animals) that is robust to genotyping and map errors. We demonstrate that it is more efficient than previous versions and other programs, particularly in the presence of genotyping errors. AVAILABILITY AND IMPLEMENTATION: The software LINKPHASE3 is included in the PHASEBOOK package and can be freely downloaded from www.giga.ulg.ac.be/jcms/prod_381171/software. The package is written in FORTRAN and contains source codes. A manual is provided with the package. CONTACT: tom.druet@ulg.ac.be SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online. [less ▲]

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See detailExperimental Evidence of Large Changes in Terrestrial Chlorine Cycling Following Altered Tree Species Composition
Montelius, Malin; Thiry, Yves; Marang, Laura et al

in Environmental Science & Technology (2015), 49

Organochlorine molecules (Clorg) are surprisingly abundant in soils and frequently exceed chloride (Cl−) levels. Despite the widespread abundance of Clorg and the common ability of microorganisms to ... [more ▼]

Organochlorine molecules (Clorg) are surprisingly abundant in soils and frequently exceed chloride (Cl−) levels. Despite the widespread abundance of Clorg and the common ability of microorganisms to produce Clorg, we lack fundamental knowledge about how overall chlorine cycling is regulated in forested ecosystems. Here we present data from a long-term reforestation experiment where native forest was cleared and replaced with five different tree species. Our results show that the abundance and residence times of Cl− and Clorg after 30 years were highly dependent on which tree species were planted on the nearby plots. Average Cl− and Clorg content in soil humus were higher, at experimental plots with coniferous trees than in those with deciduous trees. Plots with Norway spruce had the highest net accumulation of Cl− and Clorg over the experiment period, and showed a 10 and 4 times higher Cl− and Clorg storage (kg ha−1) in the biomass, respectively, and 7 and 9 times higher storage of Cl− and Clorg in the soil humus layer, compared to plots with oak. The results can explain why local soil chlorine levels are frequently independent of atmospheric deposition, and provide opportunities for improved modeling of chlorine distribution and cycling in terrestrial ecosystems. [less ▲]

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See detailAn analogue of Cobham's theorem for graph directed iterated function systems
Charlier, Emilie ULg; Leroy, Julien ULg; Rigo, Michel ULg

in Advances in Mathematics (2015), 280

Feng and Wang showed that two homogeneous iterated function systems in $\mathbb{R}$ with multiplicatively independent contraction ratios necessarily have different attractors. In this paper, we extend ... [more ▼]

Feng and Wang showed that two homogeneous iterated function systems in $\mathbb{R}$ with multiplicatively independent contraction ratios necessarily have different attractors. In this paper, we extend this result to graph directed iterated function systems in $\mathbb{R}^n$ with contraction ratios that are of the form $\frac{1}{\beta}$, for integers $\beta$. By using a result of Boigelot {\em et al.}, this allows us to give a proof of a conjecture of Adamczewski and Bell. In doing so, we link the graph directed iterated function systems to Büchi automata. In particular, this link extends to real numbers $\beta$. We introduce a logical formalism that permits to characterize sets of $\mathbb{R}^n$ whose representations in base $\beta$ are recognized by some Büchi automata. This result depends on the algebraic properties of the base: $\beta$ being a Pisot or a Parry number. The main motivation of this work is to draw a general picture representing the different frameworks where an analogue of Cobham's theorem is known. [less ▲]

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See detailTargeting cholesterol homeostasis to fight hearing loss: a new perspective.
Malgrange, Brigitte ULg; Varela-Nieto, Isabel; de Medina, Philippe et al

in Frontiers in aging neuroscience (2015), 7

Sensorineural hearing loss (SNHL) is a major pathology of the inner ear that affects nearly 600 million people worldwide. Despite intensive researches, this major health problem remains without ... [more ▼]

Sensorineural hearing loss (SNHL) is a major pathology of the inner ear that affects nearly 600 million people worldwide. Despite intensive researches, this major health problem remains without satisfactory solutions. The pathophysiological mechanisms involved in SNHL include oxidative stress, excitotoxicity, inflammation, and ischemia, resulting in synaptic loss, axonal degeneration, and apoptosis of spiral ganglion neurons. The mechanisms associated with SNHL are shared with other neurodegenerative disorders. Cholesterol homeostasis is central to numerous pathologies including neurodegenerative diseases and cholesterol regulates major processes involved in neurons survival and function. The role of cholesterol homeostasis in the physiopathology of inner ear is largely unexplored. In this review, we discuss the findings concerning cholesterol homeostasis in neurodegenerative diseases and whether it should be translated into potential therapeutic strategies for the treatment of SNHL. [less ▲]

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See detailL'environnement entre scinece et sens commun
Mormont, Marc ULg

in Natures Sciences Sociétés (2015), 23(3),

John Dewey propose dès 1948 une conception des rapports entre science et sens commun qui permet d'élucider les difficultés permanentes de l'apporche de l'environnement.

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See detailCompte rendu de 'Mutations et déterminisme chez Bourdieu'
Saint-Amand, Denis ULg

in Lectures (2015)

En définitive, il aurait sans doute mieux valu resserrer le propos sous la forme d’un article critique qui aurait pu trouver place dans une revue spécialisée : ne se donnant par les moyens de ses trop ... [more ▼]

En définitive, il aurait sans doute mieux valu resserrer le propos sous la forme d’un article critique qui aurait pu trouver place dans une revue spécialisée : ne se donnant par les moyens de ses trop hautes ambitions, l’ouvrage se révèle trop léger en plus de se distinguer par le manque de soin apporté à sa réalisation [less ▲]

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See detailEffect of metal ions and metal nanoparticles encapsulated in porous silica on biphenyl biodegradation by Rhodococcus erythropolis T902.1
Wannoussa, Wissal ULg; Hiligsmann, Serge ULg; Tasseroul, Ludivine ULg et al

in Journal of Sol-Gel Science and Technology (2015)

Biodegradation of biphenyl was carried out by Rhodococcus erythropolis T902.1 in presence ofnanometer-sized metallic (Co, Pd, Ag and Cu) nanoparticles (NPS) synthesized by the sol-gel process. In order to ... [more ▼]

Biodegradation of biphenyl was carried out by Rhodococcus erythropolis T902.1 in presence ofnanometer-sized metallic (Co, Pd, Ag and Cu) nanoparticles (NPS) synthesized by the sol-gel process. In order to prevent their agglomeration, the metallic NPs (1-2 nm diameter) were anchored inside microporous silica crystallites and named Co/SiO2, Pd/SiO2, Ag/SiO2 and Cu/SiO2 samples respectively. They were added at low concentrations of 10-6 M, 10-5 M and 10-4 M of metal in the culture medium and their impact was compared with that of the simple metal ions added as cobalt, palladium, silver or copper salts. The cultures containing Pd/SiO2 or Co/SiO2 samples at 10-4 M of metal achieved a 50% higher biphenyl degradation yield after 18 days of incubation and improved Rhodococcus erythropolis T902.1 growth compared with those without (positive control) or with silica particles only. The highest biodegradation performance, i.e. 107 ±3 ppm/day, which was about 85% higher than in control conditions without NPs, was recorded in 250 ml baffled flasks stirred at 150 rpm with Co/SiO2 sample at 10-4 M Co. Furthermore, the stimulating effect of NPs on biphenyl biodegradation seems to also depend on the thermal treatment conditions applied to NPs since the experimental results indicated that, after calcination, the cobalt oxide NPs at a concentration of 10-4 M were more effective than the reduced cobalt NPs with a degradation yield of 81 ±1% and 77 ±2% respectively after 18 days. On the other hand, the results showed that the addition of 10-4 M of Cu2+ or Ag+ ions or the addition of Cu/SiO2 or Ag/SiO2 samples at 10-4 M of metal have an inhibitory effect on biphenyl biodegradation. However, Cu2+ and Ag+ ions were more toxic to the Rhodococcus erythropolis T902.1 bacteria than the respective Cu or Ag NPS anchored inside silica particles. Moreover, this work showed that in these conditions, the activity of catechol 1, 2-dioxygenase (a critical enzyme in aromatic biodegradation pathway) was severely inhibited, whereas the presence of 10-4 M of Co2+ ions or Co/SiO2 sample stimulated the enzyme activity compared to the conditions without NPs. [less ▲]

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See detailParallel and Multivalued Logic by the Two-Dimensional Photon-Echo Response of a Rhodamine–DNA Complex
Fresch, Barbara ULg; Cipolloni, Marco; Yan, Tian-Min ULg et al

in The Journal of Physical Chemistry Letters (2015), 6

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See detailPour une iconologie critique : sens, dynamique et efficacité des images
Hagelstein, Maud ULg

in Critique d'Art : Actualité Francophone et Internationale de la Littérature Critique sur l'Art Contemporain = The International Review of Contemporary Art Criticism (2015)

La théorie de l’image actuelle s’inscrit dans le sillage de l’iconologie en vertu de la reprise explicite de plusieurs axes problématiques centraux pour cette méthode, qui permettent son redéploiement ... [more ▼]

La théorie de l’image actuelle s’inscrit dans le sillage de l’iconologie en vertu de la reprise explicite de plusieurs axes problématiques centraux pour cette méthode, qui permettent son redéploiement critique : 1. la réflexion sur la représentation iconique, sur son rapport au langage et sa logique spécifique ; 2. l’enquête sur la dynamique de l’image (circulation, persistance et développement) ; 3. l’étude de l’efficacité des images et de la production de savoirs multiples sous l’effet d’opérations de montage. [less ▲]

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See detailTernary DNA computing using 3 x 3 multiplication matrices
Orbach, Ron; Lilienthal, Sivan; Klein, Michael et al

in Chemical Science (2015), 6

Non-Boolean computations implementing operations on multi-valued variables beyond base 2 allow enhanced computational complexity. We introduce DNA as a functional material for ternary computing, and in ... [more ▼]

Non-Boolean computations implementing operations on multi-valued variables beyond base 2 allow enhanced computational complexity. We introduce DNA as a functional material for ternary computing, and in particular demonstrate the use of three-valued oligonucleotide inputs to construct a 3 [times] 3 multiplication table. The system consists of two three-valued inputs of -1; 0; +1 and a fluorophore/quencher functional hairpin acting as computational and reporter module. The interaction of the computational hairpin module with the different values of the inputs yields a 3 [times] 3 multiplication matrix consisting of nine nanostructures that are read out by three distinct fluorescence intensities. By combining three different hairpin computational modules, each modified with a different fluorophore/quencher pair, and using different sets of inputs, the parallel operation of three multiplication tables is demonstrated. [less ▲]

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See detailImproved extraction of valence transversity distributions from inclusive dihadron production
Radici, Marco; Courtoy, Aurore ULg; Bacchetta, Alessandro et al

in JHEP (2015), 1505

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See detailDevelopment of novel SOlid Materials for high power Li polymer BATteries (SOMABAT). Recyclability of components.
Zubizarreta, Leire; Gil-Agusti, Mayte; Garcia. et al

in Lecture notes on Mobility (2015)

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See detailAdded value of a triaxial accelerometer assessing gait parameters to predict falls and mortality among nursing home residents: A two-year prospective study.
Buckinx, Fanny ULg; Beaudart, Charlotte ULg; Slomian, Justine ULg et al

in Technology and health care : official journal of the European Society for Engineering and Medicine (2015), 23

BACKGROUND: Gait impairment seems to be a risk factor for falls and mortality. Because gait change cannot be determined easily with classical clinical tests, some authors have suggested that it might be ... [more ▼]

BACKGROUND: Gait impairment seems to be a risk factor for falls and mortality. Because gait change cannot be determined easily with classical clinical tests, some authors have suggested that it might be useful to use a gait-analysis system among elderly community-dwelling people. OBJECTIVE: The main objective of the present study was to determine the predictive value of a quantitative evaluation of the gait characteristics in nursing home residents for the occurrence of falls and death performed using a tri-axial accelerometer (Locometrix(R)). MATERIAL AND METHODS: One hundred elderly nursing home residents (80 women and 20 men, mean age 86.4 +/- 6.04 years) were included in this study with the aim to follow them for 2 years. Deaths and falls were systematically recorded. A quantitative evaluation of a 10-second walk was performed with a tri-axial accelerometer (Locometrix(R)). Demographic data (i.e age, sex, body mass index) and clinical data (i.e. fall risk evaluated by the Tinetti test) were also recorded. RESULTS: During the two years of follow-up, 27 patients died. After adjustment on all potential confounding variables, only body mass index was significantly associated with the risk of mortality with an odds ratio of 0.86 (95% CI: 0.77-0.96, p=0.04). At the end of the study period, 440 falls had occurred (mean: 4.44 +/- 6.79 falls per patient) but no single factors were independently associated with fall incidence. CONCLUSION: Our results show that a quantitative gait analysis performed using a tri-axial accelerometer is not predictive of long-term falls and mortality among nursing home residents. [less ▲]

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See detailSmall Angle X-ray Scattering Insights into the Architecture-Dependent Emulsifying Properties of Amphiphilic Copolymers in Supercritical Carbon Dioxide
Alaimo, David ULg; Hermida Merino, Daniel; Grignard, Bruno ULg et al

in Journal of Physical Chemistry B (2015), 119

The supramolecular assembly of a series of copolymers combining a PEO-rich hydrophilic and fluorinated CO2-philic sequences is analysed by synchrotron small-angle xray scattering (SAXS) in supercritical ... [more ▼]

The supramolecular assembly of a series of copolymers combining a PEO-rich hydrophilic and fluorinated CO2-philic sequences is analysed by synchrotron small-angle xray scattering (SAXS) in supercritical CO2, as well as in water/CO2 emulsions. These copolymers were designed to have the same molecular weight and composition, and to differ only by their macromolecular architecture. The investigated copolymers have random, block, and palm-tree architectures. Besides, thermo-responsive copolymer is also analysed, having a hydrophilic sequence becoming water-insoluble around 41 °C, i.e. just above the critical point of CO2. At the length scale investigated by SAXS, only the random copolymer appears to self-assemble in pure CO2, in the form of a disordered microgel-like network. The random, block and thermo-responsive copolymers are all able to stabilize water/CO2 emulsions but not the copolymer with the palm-tree architecture, pointing at the importance of macromolecular architecture for the emulsifying properties. A modelling of the SAXS data shows that the block and the thermo-responsive copolymers form spherical micelle-like structures containing about 70 % water and 30 % polymer. [less ▲]

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See detailNuclear Cardiology Core Syllabus of the European Association of Cardiovascular Imaging (EACVI).
Gimelli, Alessia; Neglia, Danilo; Schindler, Thomas H. et al

in European heart journal cardiovascular Imaging (2015)

The European Association of Cardiovascular Imaging (EACVI) Core Syllabus for Nuclear Cardiology is now available online. The syllabus lists key elements of knowledge in nuclear cardiology. It represents a ... [more ▼]

The European Association of Cardiovascular Imaging (EACVI) Core Syllabus for Nuclear Cardiology is now available online. The syllabus lists key elements of knowledge in nuclear cardiology. It represents a framework for the development of training curricula and provides expected knowledge-based learning outcomes to the nuclear cardiology trainees. [less ▲]

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See detailTrabecular bone score (TBS) as a new complementary approach for osteoporosis evaluation in clinical practice.
Harvey, N. C.; Gluer, C. C.; Binkley, N. et al

in Bone (2015), 78

Trabecular bone score (TBS) is a recently-developed analytical tool that performs novel grey-level texture measurements on lumbar spine dual X-ray absorptiometry (DXA) images, and thereby captures ... [more ▼]

Trabecular bone score (TBS) is a recently-developed analytical tool that performs novel grey-level texture measurements on lumbar spine dual X-ray absorptiometry (DXA) images, and thereby captures information relating to trabecular microarchitecture. In order for TBS to usefully add to bone mineral density (BMD) and clinical risk factors in osteoporosis risk stratification, it must be independently associated with fracture risk, readily obtainable, and ideally, present a risk which is amenable to osteoporosis treatment. This paper summarizes a review of the scientific literature performed by a Working Group of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis. Low TBS is consistently associated with an increase in both prevalent and incident fractures that is partly independent of both clinical risk factors and areal BMD (aBMD) at the lumbar spine and proximal femur. More recently, TBS has been shown to have predictive value for fracture independent of fracture probabilities using the FRAX(R) algorithm. Although TBS changes with osteoporosis treatment, the magnitude is less than that of aBMD of the spine, and it is not clear how change in TBS relates to fracture risk reduction. TBS may also have a role in the assessment of fracture risk in some causes of secondary osteoporosis (e.g. diabetes, hyperparathyroidism and glucocorticoid-induced osteoporosis). In conclusion, there is a role for TBS in fracture risk assessment in combination with both aBMD and FRAX. [less ▲]

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See detailFarmers' practices and willingness to adopt supplemental irrigation in Burkina Faso
Zongo, Bétéo ULg; Diarra, Abdoulaye; Barbier, Bruno et al

in International Journal of Food and Agricultural Economics (2015), 3(1), 101-117

This study analyzes farmers' practices and willingness to adopt supplemental irrigation on rainfed crops in the Sahelian and Sudano-Sahelian areas of Burkina Faso. A survey of 629 farmers revealed a ... [more ▼]

This study analyzes farmers' practices and willingness to adopt supplemental irrigation on rainfed crops in the Sahelian and Sudano-Sahelian areas of Burkina Faso. A survey of 629 farmers revealed a diversity of farming practices within and between two agro-climatic zones. Over 65% of farmers believe that supplemental irrigation is a good way to mitigate the adverse effects of dry spells on crop yields. Determining factors of farmers’ willingness to adopt supplemental irrigation were identified using a logistic model. Lack of information is the major obstacle to promoting this innovation. Farmers’ training and information dissemination are the best ways to increase the acceptance of this promising technology. [less ▲]

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See detailEACVI/HFA Cardiac Oncology Toxicity Registry in breast cancer patients: rationale, study design, and methodology (EACVI/HFA COT Registry)-EURObservational Research Program of the European Society of Cardiology.
Lancellotti, Patrizio ULg; Anker, Stefan D.; Donal, Erwan et al

in European heart journal cardiovascular Imaging (2015)

The goal of adjuvant anti-cancer therapies is cure with limited or no side effects, in particular long-term side effects with negative impact on quality of life. In the palliative setting disease control ... [more ▼]

The goal of adjuvant anti-cancer therapies is cure with limited or no side effects, in particular long-term side effects with negative impact on quality of life. In the palliative setting disease control, quality of life and overall survival are important end points. Partly due to improvements in treatment, the population of cancer survivors is large and growing. However, anti-cancer drug-related cardiotoxicity (ADRC) is the leading cause of treatment-associated mortality in cancer survivors. It is one of the most common post-treatment problems among 5- to 10-year survivors of adult cancer. This is particularly true for breast cancer, the most common cancer in women. The EACVI/HFA COT registry is designed for comprehensive data collection and evaluation of the current European practice in terms of diagnosis and management of ADRC in breast cancer patients. The COT registry will be carried out in two continuing phases, the pilot study phase involving 13 countries followed by the long-term registry in which all the 56 ESC countries will be invited to participate. With the COT registry, several critical information will be obtained: on predisposing factors for the development of ADRC, the rate of subclinical LV dysfunction and its transition to overt heart failure, the clinical impact and outcome of ADRC. [less ▲]

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See detailWhen private actors matter: Information-sharing network and surveillance of Highly Pathogenic Avian Influenza in Vietnam
Delabouglise, Alexis; Dao, T.H.; Truong, D.B. et al

in Acta Tropica (2015), 147

The effectiveness of animal health surveillance systems depends on their capacity to gather sanitary information from the animal production sector. In order to assess this capacity we analyzed the flow of ... [more ▼]

The effectiveness of animal health surveillance systems depends on their capacity to gather sanitary information from the animal production sector. In order to assess this capacity we analyzed the flow of sanitary information regarding Highly Pathogenic Avian Influenza (HPAI) suspicions in poultry in Viet- nam. Participatory methods were applied to assess the type of actors and likelihood of information sharing between actors in case of HPAI suspicion in poultry. While the reporting of HPAI suspicions is manda- tory, private actors had more access to information than public actors. Actors of the upstream sector (medicine and feed sellers) played a key role in the diffusion of information. The central role of these actors and the influence of the information flow on the adoption by poultry production stakeholders of behaviors limiting (e.g. prevention measures) or promoting disease transmission (e.g. increased animal movements) should be accounted for in the design of surveillance and control programs. [less ▲]

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See detailTowards a framework for assessment and management of cumulative human impacts on marine food webs
Giakoumi, Sylvaine; Halpern, Benjamin S.; Michel, Loïc ULg et al

in Conservation Biology (2015), 00(0), 1-7

Effective ecosystem-based management requires understanding ecosystem responses to multiple human threats, rather than focusing on single threats. To understand ecosystem responses to anthropogenic ... [more ▼]

Effective ecosystem-based management requires understanding ecosystem responses to multiple human threats, rather than focusing on single threats. To understand ecosystem responses to anthropogenic threats holistically, it is necessary to know how threats affect different components within ecosystems and ultimately alter ecosystem functioning. We used a case study of a Mediterranean seagrass (Posidonia oceanica) food web and expert knowledge elicitation in an application of the initial steps of a framework for assessment of cumulative human impacts on food webs. We produced a conceptual seagrass food web model, determined the main trophic relationships, identified the main threats to the food web components, and assessed the components’ vulnerability to those threats. Some threats had high (e.g., coastal infrastructure) or low impacts (e.g., agricultural runoff) on all food web components, whereas others (e.g., introduced carnivores) had very different impacts on each component. Partitioning the ecosystem into its components enabled us to identify threats previously overlooked and to reevaluate the importance of threats commonly perceived as major. By incorporating this understanding of system vulnerability with data on changes in the state of each threat (e.g., decreasing domestic pollution and increasing fishing) into a food web model, managers may be better able to estimate and predict cumulative human impacts on ecosystems and to prioritize conservation actions. [less ▲]

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See detailMise en perspective de l'après-midi de rencontre avec les maîtres de stages du Cifen
Simons, Germain ULg

in Didactiques en pratique (2015), 1

Ce texte est le discours du Président du Cifen qui a été prononcé à l'occasion de la première journée officielle de rencontre entre le Centre et les maitres de stages (5 novembre 2014). Ce discours ... [more ▼]

Ce texte est le discours du Président du Cifen qui a été prononcé à l'occasion de la première journée officielle de rencontre entre le Centre et les maitres de stages (5 novembre 2014). Ce discours succinct reprend la question qui a servi de fil rouge à cette journée : "Comment collaborer efficacement pour accompagner au mieux nos stagiaires ?" et en détaille chaque composante. [less ▲]

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See detailThe year in cardiology 2014: valvular heart disease.
Pierard, Luc ULg; Vahanian, Alec

in European heart journal (2015)

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See detailUncertainties in Models of Stellar Structure and Evolution
Noels-Grötsch, Arlette ULg; Bragaglia, Angela

in Astrophysics and Space Science Proceedings (2015), 39

Numerous physical aspects of stellar physics have been presented in Session 2 and the underlying uncertainties have been tentatively assessed. We try here to highlight some specific points raised after ... [more ▼]

Numerous physical aspects of stellar physics have been presented in Session 2 and the underlying uncertainties have been tentatively assessed. We try here to highlight some specific points raised after the talks and during the general discussion at the end of the session and eventually at the end of the workshop. A table of model uncertainties is then drawn with the help of the participants in order to give the state of the art in stellar modeling uncertainties as of July 2013. [less ▲]

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See detailThe enfranchisement of citizens abroad: variations and explanations
Lafleur, Jean-Michel ULg

in Democratization (2015)

Today, a large majority of states allow at least some of their emigrants to take part in home country elections from abroad. This article first looks at the diffusion of external voting laws and shows ... [more ▼]

Today, a large majority of states allow at least some of their emigrants to take part in home country elections from abroad. This article first looks at the diffusion of external voting laws and shows that over the past 25 years they have become widely-adopted and are no longer limited to specific professional categories of citizens. Second, the article explains the international diffusion of external voting by discussing the “norm- internationalization hypothesis” and the “electoral-competition hypothesis.” Third, the article attempts to demonstrate that these hypotheses cannot explain why, in a democratic context, states continue to implement a series of hurdles that deter emigrants from using their newly gained rights. Looking at recent developments in Latin America and the Middle East and North Africa, it concludes that the diffusion and variations of external voting laws result from transnational negotiation processes in a context of democratic transformation among various actors whose interests are strongly affected by the inclusion or exclusion of these new voters. [less ▲]

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See detailBioaccumulation of organohalogenated compounds in sharks and rays from the southeastern USA
Weijs, Liesbeth; Briels, Nathalie; Adams, Douglas et al

in Environmental Research (2015), 137

Organohalogenated compounds are widespread in the marine environment and can be a serious threat to organisms in all levels of aquatic food webs, including elasmobranch species. Information about the ... [more ▼]

Organohalogenated compounds are widespread in the marine environment and can be a serious threat to organisms in all levels of aquatic food webs, including elasmobranch species. Information about the concentrations of POPs (persistent organic pollutants) and of MeO-PBDEs (methoxylated polybrominated diphenyl ethers) in elasmobranchs is scarce and potential toxic effects are poorly understood. The aims of the present study were therefore to investigate the occurrence of multiple POP classes (PCBs, PBDEs, DDXs, HCB, CHLs) and of MeO-PBDEs in various elasmobranch species from different trophic levels in estuarine and marine waters of the southeastern United States. Overall, levels and patterns of PCBs, PBDEs, DDXs, HCB, CHLs and of MeO-PBDEs varied according to the species, maturity stage, gender and habitat type. The lowest levels of POPs were found in Atlantic stingrays and the highest levels were found in bull sharks. As both species are respectively near the bottom and at top of the trophic web, with juvenile bull sharks frequently feeding on Atlantic stingrays, these findings further suggest a bioaccumulation and biomagnification process with trophic position. MeO-PBDEs were not detected in Atlantic stingrays, but were found in all shark species. HCB was not found in Atlantic stingrays, bonnetheads or lemon sharks, but was detected in the majority of bull sharks examined. Comparison with previous studies suggests that Atlantic stingrays may be experiencing toxic effects of PCBs and DDXs on their immune system. However, the effect of these compounds on the health of shark species remains unclear. [less ▲]

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See detailAdventitious organogenesis induced in sweet orange (Citrus sinensis L.) var. "half-blood" maltese: morphogenetic and histological study
Benmahmoud, Kaouther; Jedidi, Zmna; Najar, Asma et al

in International Journal of Agronomy and Agricultural Research (2015), 6(2), 1-7

Tunisian citrus crops are faced to several abiotic and biotic constraints among which virus and virus-like diseases are incurable. The production of virus-free plants systematically needs the use of in ... [more ▼]

Tunisian citrus crops are faced to several abiotic and biotic constraints among which virus and virus-like diseases are incurable. The production of virus-free plants systematically needs the use of in vitro techniques. In this context, somatic embryogenesis and further plantlet regeneration of the Tunisian “half-blood” Maltese orange were obtained using explants consisting in style/stigma collected from unopened flowers. Somatic embryos were induced on Murashige and Skoog medium containing 13.3 µM 6-benzylaminopurine and 500 mg.l-1 malt extract, but their germination was obtained on hormone free-medium. Somatic embryogenesis was induced indirectly from intermediate friable callus initiated at the basal part of the style. Somatic embryos exhibited central procambial cells and were surrounded by a protoderm isolating them from the callus. These embryos had bipolar structure confirmed by the presence of shoot and root apices at cotyledonary stage. The use of cotyledon excised from those embryos failed to regenerate somatic embryos, but gave rise to direct organogenesis in two forms, true buds and protuberances both evolved in shoots after transfer in hormone-free medium. According to histological observations, protuberances are induced from epidermal and subepidermal cells of the cotyledon explant and remain closely attached to their mother tissue even at the shoot stage. [less ▲]

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See detailGlioblastoma Circulating Cells: Reality, Trap or Illusion?
LOMBARD, Arnaud ULg; Goffart, Nicolas ULg; Rogister, Bernard ULg

in Stem Cells International (2015)

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See detailFinite element modelling of composite structures under crushing load
Chiu, Louis N.S.; Falzon, Brian G.; Boman, Romain ULg et al

in Composite Structures (2015)

This paper details the theory and implementation of a composite damage model, addressing damage within a ply (intralaminar) and delamination (interlaminar), developed for the simulation of the crushing of ... [more ▼]

This paper details the theory and implementation of a composite damage model, addressing damage within a ply (intralaminar) and delamination (interlaminar), developed for the simulation of the crushing of laminated composite structures. It includes a more accurate determination of the characteristic length to achieve mesh objectivity in capturing intralaminar damage consisting of matrix cracking and fibre failure, a load-history dependent material response, an isotropic hardening nonlinear matrix response, as well as a more physically-based interactive matrix damage mechanism. The developed damage model requires a set of material parameters obtained from a combination of standard and non-standard material characterisation tests. The fidelity of the model mitigates the need to manipulate, or “calibrate”, the input data to achieve good agreement with experimental results. This intralaminar damage model was implemented as a VUMAT subroutine, and used in conjunction with an existing interlaminar damage model, in Abaqus/Explicit. This approach was validated through the simulation of the crushing of a cross-ply composite tube with a tulip-shaped trigger, loaded in uniaxial compression. Despite the complexity of the chosen geometry, excellent correlation was achieved with experimental results. [less ▲]

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See detailShort communication: Novel method to predict body weight of primiparous dairy cows throughout the lactation
Vanrobays, Marie-Laure ULg; Vandenplas, Jérémie ULg; Hammami, Hedi ULg et al

in Journal of Dairy Science (2015), 98

Body weight (BW) of dairy cows can be estimated using linear conformation traits (calculated BW; CBW), which are generally recorded only once during a lactation. However, predicted BW (PBW) throughout the ... [more ▼]

Body weight (BW) of dairy cows can be estimated using linear conformation traits (calculated BW; CBW), which are generally recorded only once during a lactation. However, predicted BW (PBW) throughout the lactation would be useful, e.g., at milk-recording dates allowing feed-intake prediction for advisory purposes. Therefore, a 2-step approach was developed to obtain PBW for each milk-recording date. In the first step, a random-regression test-day model was used with CBW as observations to predict PBW. The second step consisted in changing means and (co)variances of prior distributions for the additive genetic random effects of the test-day model by using priors derived from results of the first step to predict again PBW. A total of 25,061 CBW from 24,919 primiparous Holstein cows were computed using equations from literature. Using CBW as observations, PBW was then predicted over the whole lactation for 232,436 dates corresponding to 207,375 milk-recording dates and 25,061 classification dates. Results showed that using both steps (the 2-step approach) provided more accurate predictions than using only the first step (the one-step approach). Based on the results of this preliminary study, BW of dairy cows could be predicted throughout the lactation using this procedure. These predictions could be useful in milk-recording systems to compute traits of interest (e.g., feed-intake prediction). The developed novel method is also flexible because actual direct measurements of BW can also be used together with CBW, the prediction model being able to accommodate different levels of accuracies of used BW phenotypes. [less ▲]

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See detailEffect of enzymatic treatment on rheological properties, glass temperature transition and microstructure of date syrup
Abbès, Fatma; Masmoudi, Manel; Kchaou, Wissal et al

in LWT - Food Science and Technology (2015), 60

A knowledge of rheological properties is of importance in processing, handling, process design, product development and quality control. This study investigates the effect of commercial hydrolytic enzymes ... [more ▼]

A knowledge of rheological properties is of importance in processing, handling, process design, product development and quality control. This study investigates the effect of commercial hydrolytic enzymes (pectinase and cellulase) on rheological properties and glass temperature transition of date syrup. Date syrups obtained by enzymatic extraction exhibited a quasi Newtonian behaviour. The enzyme-treated date syrups showed the highest values of activation energy (Ea). Therefore, these syrups showed the most temperature dependency. Dynamic shear results revealed viscous behaviour for date syrups indicating that both syrups displayed liquid-like behaviour. Temperature significantly affected both (G') and (G''). The glass transition temperature (Tg) of date syrups varied between -39.56 and -45.74 °C depending on their composition. Glass transition temperature of date syrups decreased linearly with an increase in water content. [less ▲]

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See detailA novel form of ficin from Ficus carica latex: Purification and characterization
Baeyens-Volant, Danielle; Matagne, André ULg; El Mahyaoui, Rachida et al

in Phytochemistry (2015), 117

A novel ficin form, named ficin E, was purified from fig tree latex by a combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and fplc-gel filtration ... [more ▼]

A novel ficin form, named ficin E, was purified from fig tree latex by a combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and fplc-gel filtration chromatography. The new ficin appeared not to be sensitive to thiol derivatization by a polyethylene glycol derivative, allowing its purification. The protease is homogeneous according to PAGE, SDS–PAGE, mass spectrometry, N-terminal micro-sequencing analyses and E-64 active site titration. N-terminal sequencing of the first ten residues has shown high identity with the other known ficin (iso)forms. The molecular weight was found to be (24,294 ± 10) Da by mass spectrometry, a lower value than the apparent molecular weight observed on SDS–PAGE, around 27 kDa. Far-UV CD data revealed a secondary structure content of 22% a-helix and 26% b-sheet. The protein is not glycosylated as shown by carbohydrate analysis. pH and temperature measurements indicated maxima activity at pH 6.0 and 50 °C, respectively. Preliminary pH stability analyses have shown that the protease conserved its compact structure in slightly acidic, neutral and alkaline media but at acidic pH (<3), the formation of some relaxed or molten state was evidenced by 8-anilino-1-naphtalenesulfonic acid binding characteristics. Comparison with the known ficins A, B, C, D1 and D2 (iso)forms revealed that ficin E showed activity profile that looked like ficin A against two chromogenic substrates while it resembled ficins D1 and D2 against three fluorogenic substrates. Enzymatic activity of ficin E was not affected by Mg2+, Ca2+ and Mn2+ at a concentration up to 10 mM. However, the activity was completely suppressed by Zn2+ at a concentration of 1 mM. Inhibitory activity measurements clearly identified the enzyme as a cysteine protease, being unaffected by synthetic (Pefabloc SC, benzamidine) and by natural proteinaceous (aprotinin) serine proteases inhibitors, by aspartic proteases inhibitors (pepstatin A) and by metallo-proteases inhibitors (EDTA, EGTA). Surprisingly, it was well affected by the metallo-protease inhibitor o-phenanthroline. The enzymatic activity was however completely blocked by cysteine proteases inhibitors (E-64, iodoacetamide), by thiol-blocking compounds (HgCl2) and by cysteine/serine proteases inhibitors (TLCK and TPCK). This is a novel ficin form according to peptide mass fingerprint analysis, specific amidase activity, SDS–PAGE and PAGE electrophoretic mobility, N-terminal sequencing and unproneness to thiol pegylation. [less ▲]

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See detailOak barks as raw materials for the extraction of polyphenols for the chemical and pharmaceutical sectors: a regional case study
Dedrie, Maxime; Jacquet, Nicolas ULg; Bombeck, Pierre-Louis ULg et al

in Industrial Crops and Products (2015), 70

Despite their potential for chemical recycling, residues from forest harvesting and wood processing are mostly used for industrial applications with low added value (energy, paper pulp, panels). Bark of ... [more ▼]

Despite their potential for chemical recycling, residues from forest harvesting and wood processing are mostly used for industrial applications with low added value (energy, paper pulp, panels). Bark of both oak species, Quercus robur L. 1753, Quercus petraea (Matt.) Liebl. 1784 is a by-product from sawmill and pulp mill activities. Bark is mainly used as a fuel for the same wood plants. The aim of this study is to look at the feasibility of enhancing the value of this material through the extraction of bioactive molecules such as polyphenols (i.e. catechin, gallic and ellagic acids). First, the effect of industrial storage of logs and bark on their polyphenol content was explored. Then, referring to the selection of tan oaks in the past, the question of an optimum harvesting age is addressed in order to maximize the polyphenol content of the barks. In the end, molecular diversity of bark is examined through the identification of molecules of interest, using different chromatographic analyses. The results show an effect of the industrial context and an effect of the raw material age on the chemical properties of the bark. First investigations also highlight molecules of interest and the molecular diversity, which needs to be further explored. [less ▲]

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See detailEndothelial exosomes contribute to the antitumor response during breast cancer neoadjuvant chemotherapy via microRNA transfer.
Bovy, Nicolas ULg; Blomme, Benoît ULg; Freres, Pierre ULg et al

in Oncotarget (2015)

The interaction between tumor cells and their microenvironment is an essential aspect of tumor development. Therefore, understanding how this microenvironment communicates with tumor cells is crucial for ... [more ▼]

The interaction between tumor cells and their microenvironment is an essential aspect of tumor development. Therefore, understanding how this microenvironment communicates with tumor cells is crucial for the development of new anti-cancer therapies. MicroRNAs (miRNAs) are small non-coding RNAs that inhibit gene expression. They are secreted into the extracellular medium in vesicles called exosomes, which allow communication between cells via the transfer of their cargo. Consequently, we hypothesized that circulating endothelial miRNAs could be transferred to tumor cells and modify their phenotype. Using exogenous miRNA, we demonstrated that endothelial cells can transfer miRNA to tumor cells via exosomes. Using miRNA profiling, we identified miR-503, which exhibited downregulated levels in exosomes released from endothelial cells cultured under tumoral conditions. The modulation of miR-503 in breast cancer cells altered their proliferative and invasive capacities. We then identified two targets of miR-503, CCND2 and CCND3. Moreover, we measured increased plasmatic miR-503 in breast cancer patients after neoadjuvant chemotherapy, which could be partly due to increased miRNA secretion by endothelial cells. Taken together, our data are the first to reveal the involvement of the endothelium in the modulation of tumor development via the secretion of circulating miR-503 in response to chemotherapy treatment. [less ▲]

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See detailMultiple timescale spectral analysis
Denoël, Vincent ULg

in Probabilistic Engineering Mechanics (2015), 39(0), 69--86

Abstract Spectral analysis is a classical tool for the structural analysis of structures subjected to random excitations. The most common application of spectral analysis is the determination of the ... [more ▼]

Abstract Spectral analysis is a classical tool for the structural analysis of structures subjected to random excitations. The most common application of spectral analysis is the determination of the steady-state second order cumulant of a linear oscillator, under the action of a stationary loading prescribed by means of its power spectral density. There exists however a broad variety of such similar problems, extending the concept to multi degree-of-freedom systems, non Gaussian excitation, slightly nonlinear oscillators or even transient excitations. In this wide class of problems, the cumulants of the response are obtained as the result of the integral of corresponding spectra over the frequency space, which is possibly multidimensional. Application of standard numerical integration techniques may be prohibitive, a reason why the spectral approach is often left aside. Besides, many engineering problems involve a clear timescale separation, usually of those pertaining to the loading and to the mechanical behavior of the system. In these problems, a proper consideration of the timescale separation results in dropping the order of integration by one, at least. This offers the possibility to derive analytical solutions, whenever the order of integration drops to zero, or to make numerical integration competitive. The paper presents this general method, together with some applications in wind and marine engineering. [less ▲]

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See detailCombining tree-based and dynamical systems for the inference of gene regulatory networks
Huynh-Thu, Vân Anh ULg; Sanguinetti, Guido

in Bioinformatics (2015), 31(10), 1614-1622

Motivation: Reconstructing the topology of gene regulatory networks (GRNs) from time series of gene expression data remains an important open problem in computational systems biology. Existing GRN ... [more ▼]

Motivation: Reconstructing the topology of gene regulatory networks (GRNs) from time series of gene expression data remains an important open problem in computational systems biology. Existing GRN inference algorithms face one of two limitations: model-free methods are scalable but suffer from a lack of interpretability and cannot in general be used for out of sample predictions. On the other hand, model-based methods focus on identifying a dynamical model of the system. These are clearly interpretable and can be used for predictions; however, they rely on strong assumptions and are typically very demanding computationally. Results: Here, we propose a new hybrid approach for GRN inference, called Jump3, exploiting time series of expression data. Jump3 is based on a formal on/off model of gene expression but uses a non-parametric procedure based on decision trees (called "jump trees") to reconstruct the GRN topology, allowing the inference of networks of hundreds of genes. We show the good performance of Jump3 on in silico and synthetic networks and applied the approach to identify regulatory interactions activated in the presence of interferon gamma. Availability and implementation: Our MATLAB implementation of Jump3 is available at http:// homepages.inf.ed.ac.uk/vhuynht/software.html. [less ▲]

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See detailActive faulting at the western tip of the Gulf of Corinth, Greece, from high-resolution seismic data
Beckers, Arnaud ULg; Hubert, Aurelia ULg; Beck, Christian et al

in Marine Geology (2015)

The Gulf of Corinth is one of the fastest-spreading intra-continental rifts on Earth. GPS data indicate that the rift is currently opening in a NNE-SSW direction, with a rate of extension reaching up to ... [more ▼]

The Gulf of Corinth is one of the fastest-spreading intra-continental rifts on Earth. GPS data indicate that the rift is currently opening in a NNE-SSW direction, with a rate of extension reaching up to 16 mm yr-1 in its westernmost part. Although the rest of the offshore rift has been well studied, the western tip of the rift is still poorly explored. We present an accurate map of submarine faults in this area based on two high-resolution seismic reflection surveys (single channel sparker). In the eastern part of the studied area, the sedimentary infill is affected by the known North Eratini, South Eratini and West Channel faults. Further to the west, the seafloor is mostly flat, and is bounded to the north by the normal, south-dipping, Trizonia fault. To the north, the shallower part of the Gulf shows to the east a diffuse pattern of normal and strike-slip deformation which is replaced to the west by a 7.5 km long SE striking strike-slip fault zone, called the Managouli fault zone. To the westernmost tip of the Gulf, in the Nafpaktos Basin, two fault sets with different strikes are encountered; the one with aNE-SW strike exhibits a clear strike-slip component. The western tip of the Gulf of Corinth is the only part of the Corinth Rift where convincing evidence for strike-slip movement has been found. This fault pattern is likely related to the complex deformation occurring at the diffuse junction at the western tip of the Rift between three crustal blocks: Continental Greece, Peloponnese, and the Ionian Island-Akarnia block. [less ▲]

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See detailRetour au sport après plastie du ligament croisé antérieur : critères utilisés dans les cubs professionnels de football
Delvaux, François ULg; Rochcongar, P; Bruyère, Olivier ULg et al

in Science et Sports (2015), 30

Résumé Objectifs Analyser la démarche suivie par les médecins de clubs professionnels de football lorsqu’ils décident, en pratique quotidienne, d’autoriser le retour compétitif après plastie du ligament ... [more ▼]

Résumé Objectifs Analyser la démarche suivie par les médecins de clubs professionnels de football lorsqu’ils décident, en pratique quotidienne, d’autoriser le retour compétitif après plastie du ligament croisé antérieur chez un footballeur. Matériels et méthodes Trente-sept médecins responsables de clubs professionnels de football français et belges (Ligue 1, n = 15 ; Ligue 2, n = 14 ; Division 1 belge, n = 8) ont rempli un questionnaire à choix multiple concernant : (1) les critères utilisés afin de déterminer si un joueur est apte à reprendre la compétition après plastie du ligament croisé antérieur ; (2) l’importance relative de chacun de ces critères ; (3) le rôle éventuel d’intervenants spécifiques (kinésithérapeute…) dans cette décision. Résultats Plus de 80 % des médecins interrogés ont déclaré utiliser au moins huit critères (sur 17 proposés) afin d’évaluer la capacité d’un footballeur à reprendre la compétition après plastie du ligament croisé antérieur. Les trois critères considérés comme les plus déterminants étaient, par ordre d’importance : la stabilité dynamique du genou lors d’un exercice spécifique au football, la force musculaire et la récupération complète ou quasi complète d’amplitudes articulaires de flexion et d’extension de genou. Pour certains de ces critères (notamment la force musculaire), nous constatons cependant un manque de consensus sur les modalités pratiques d’évaluation, sur les paramètres ainsi que sur les valeurs-limites tolérées afin de garantir un retour sur terrain sécurisé. La prise en compte de l’avis du kinésithérapeute et du préparateur physique par une très nette majorité de médecins souligne l’importance d’un travail pluridisciplinaire. Conclusion L’utilisation de différents critères objectifs afin d’autoriser le retour compétitif après plastie du ligament croisé antérieur semble être une réalité dans le football professionnel. Des études supplémentaires devraient cependant contribuer à préciser les modalités des épreuves ainsi que des valeurs-seuils. ________________________________________ Summary Purpose To analyze how sport physicians decide, in their daily practice, when a professional soccer player with a reconstructed anterior cruciate ligament is able to get back to competitive activities. Materials and methods Thirty-seven physicians for professional French and Belgian soccer teams filled in a specific questionnaire dedicated to: (1) return-to-play criteria after anterior cruciate reconstruction; (2) the importance they assigned to each of these criteria in the return-to-play decision; (3) the potential role of professionals, such as physiotherapists or physical coaches in this decision. Results More than 80% of the respondents declared to use at least eight criteria in order to assess the player's ability to return to competitive soccer after anterior cruciate ligament reconstruction. The most important ones were (in order of importance): dynamic knee stability during a specific soccer exercise, muscle strength performance and normalization of knee flexion and extension ranges of motion. For most of these criteria (notably muscle strength), there was a lack of consensus about the choice of assessment parameters and the limit values allowing physicians to authorize or forbid the return-to-competition. A large majority of participants stated to take into consideration advices from physiotherapists or physical coaches for the return-to-play decision. Conclusion Sport physicians of professional soccer teams use relevant criteria to assess players’ ability to return to full sport after anterior cruciate ligament reconstruction. Further studies are required to determine the choice of assessment parameters and the limit values to assist physicians in return-to-play decisions. [less ▲]

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See detailNoncollinear magnetism in post-perovskites from first-principles: Comparison between CaRhO3 and NaNiF3
Garcia Castro, Andrés Camilo ULg; Romero, Aldo; Bousquet, Eric ULg

in Physica Status Solidi B. Basic Research (2015), 252(4), 689

Based on first-principles calculations, we study the noncollinear magnetism in the post-perovskites (pPv) phase of NaNiFinline image and CaRhOinline image crystals. We find that the magnetic canting is ... [more ▼]

Based on first-principles calculations, we study the noncollinear magnetism in the post-perovskites (pPv) phase of NaNiFinline image and CaRhOinline image crystals. We find that the magnetic canting is one of the most promising properties of pPv systems, which is allowed by symmetry in all the pPv crystals inline image with a magnetically active B-site. In the pPv phase of NaNiFinline image, which has a inline image antiferromagnetic ground state with a inline image ferromagnetic canting, we obtain a magnetic canting amplitude of about 0.1 inline imageatominline image, which is much larger than in the one obtained in CaRhOinline image (canting amplitude of 0.04 inline image). We also computed the exchange constants (inline image), the single-ion anisotropy (SIA) parameters and the anti-symmetric magnetic coupling described by the Dzyaloshinsky–Moriya (DM) interaction in order to scrutinize the origin of the magnetic canting. We find that the canting in NaNiFinline image is mainly due to the DM interaction, while in CaRhOinline image, both DM and SIA contribute to the magnetic canting. Our calculations thus confirm the noncollinear magnetic ground-state solution experimentally observed in both compounds and the calculated magnetic exchange interactions also confirm the quasi-2D magnetic behavior reported in pPv. [less ▲]

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See detailInfluence of mouse strain on ovarian tissue recovery after engraftment with angiogenic factor.
Fransolet, Maïté ULg; Henry, Laurie ULg; Labied, Soraya et al

in Journal of Ovarian Research (2015), 8(1), 14

BACKGROUND: For women facing gonadotoxic treatment, cryopreservation of ovarian tissue with subsequent retransplantation during remission is a promising technique for fertility preservation. However ... [more ▼]

BACKGROUND: For women facing gonadotoxic treatment, cryopreservation of ovarian tissue with subsequent retransplantation during remission is a promising technique for fertility preservation. However, follicle loss within grafted ovarian tissue can be caused by ischemia and progressive revascularization. Several xenograft models using different immunodeficient rodent lines are suitable for studying ovarian tissue survival and follicular viability after frozen-thawed ovarian cortex transplantation. SCID mice, which are deficient for functional B and T cells, are the most commonly used mice for ovarian xenograft studies. However, due to incomplete immunosuppression, NOD-SCID mice displaying low NK cell function and an absence of circulating complement might be more appropriate. The present study aims to define the most appropriate immunodeficient mouse strain for ovarian tissue xenotransplantation by comparing ovarian graft recovery in SCID and NOD-SCID mice following engraftment in the presence of isoform 111 of vascular endothelial growth factor. METHODS: Sheep ovarian cortex fragments were embedded in a collagen matrix, with or without VEGF111, before being stitched onto the ovaries of SCID and NOD-SCID mice. Transplants were recovered after 3 days to study early revascularization or after 3 weeks to evaluate follicle preservation and tissue fibrosis through histological analyses. RESULTS: At day 3, vessels were largely reorganized in the ovarian grafts of both mouse strains. After 3 weeks, the cortical tissue was clearly identifiable in SCID mice but not in NOD-SCID mice. Upon VEGF111 treatment, vascularization was significantly improved 3 days after transplantation in SCID mice. This increase in vessel density was correlated with better follicular preservation in SCID mice 3 weeks after transplantation. Fibrosis was not decreased by VEGF treatment in either mouse strain. CONCLUSIONS: Tissue architecture and follicular morphology were better preserved in ovarian tissues grafted in SCID mice in comparison with NOD-SCID mice. Moreover, tissue revascularization was improved in SCID mice by VEGF111 graft treatment. Thus, we consider SCID mice to be the best murine model for studying ovarian tissue xenografts. [less ▲]

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See detailMarine space ecology and seagrasses. Does patch type matter in Posidonia oceanica seascapes?
Abadie, Arnaud ULg; Gobert, Sylvie ULg; Bonacorsi, Marina et al

in Ecological Indicators (2015), 57

The use of landscape tools in the study of seagrass meadows (seascapes) begins to be widely spreadbut still require the establishment of several basis, i.e. a patch type classification based on ... [more ▼]

The use of landscape tools in the study of seagrass meadows (seascapes) begins to be widely spreadbut still require the establishment of several basis, i.e. a patch type classification based on numericalcharacteristics. Thanks to the complex seascapes created by the Posidonia oceanica meadows, they appearto be suitable for a study at a patch type level (class), which bring a new insight of their arrangement at thewhole seascape scale. By interpreting side scan sonar images from the Corsican coast (France) through aGIS software, it was possible to describe 11 types of patches and to evaluate their natural or anthropogenicorigin. Comparison of different landscape metrics and wave exposure (Relative wave Exposure Index,REI) at the seascape and the patch level showed that the particularity of P. oceanica seascapes are mainlycharacterized by certain types of patches often of anthropogenic origin. Furthermore the REI seems not tobe a relevant index for a study at a class scale. A bathymetrical succession of natural patches was outlinedfrom the lower to the upper limit of the meadow, with a long-term dynamic opposed to a shorter oneconcerning anthropogenic patches. In order to assess the origin (natural or induced by human activities)of the patches in P. oceanica meadows, as well as in any other seagrass, a Patchiness Source Index (PaSI),ranging from 0 to 1, was defined. [less ▲]

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See detailA Low-Cost Digital Image Correlation Technique for Characterising the Shear Deformation of Fabrics for Draping Studies
Pierce, R. S.; Falzon, B. G.; Thompson, M. C. et al

in Strain (2015)

A novel digital image correlation (DIC) technique has been developed to track changes in textile yarn orientations during shear characterisation experiments, requiring only low-cost digital imaging ... [more ▼]

A novel digital image correlation (DIC) technique has been developed to track changes in textile yarn orientations during shear characterisation experiments, requiring only low-cost digital imaging equipment. Fabric shear angles and effective yarn strains are calculated and visualised using this new DIC technique for bias extension testing of an aerospace grade, carbon-fibre reinforcement material with a plain weave architecture. The DIC results are validated by direct measurement, and the use of a wide bias extension sample is evaluated against a more commonly used narrow sample. Wide samples exhibit a shear angle range 25% greater than narrow samples and peak loads which are 10 times higher. This is primarily due to excessive yarn slippage in the narrow samples; hence, the wide sample configuration is recommended for characterisation of shear properties which are required for accurate modelling of textile draping [less ▲]

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See detailEfficient 3D transfer operators based on numerical integration
Bussetta, Philippe ULg; Boman, Romain ULg; Ponthot, Jean-Philippe ULg

in International Journal for Numerical Methods in Engineering (2015), 102(3-4), 892-929

This paper deals with data transfer between two meshes as it happens in a finite element context when a remeshing has to be performed. We propose a finite-volume-based data transfer method for an ... [more ▼]

This paper deals with data transfer between two meshes as it happens in a finite element context when a remeshing has to be performed. We propose a finite-volume-based data transfer method for an efficient remeshing of three-dimensional solid mechanics problems. The originality of this transfer method stems from a linear reconstruction of the fields to be transferred on an auxiliary finite volume mesh, a fast computation of the transfer operator and the application to the complete remeshing of 3D problems. This procedure is applicable to both nodal values and discrete fields defined at quadrature points. In addition, a data transfer method using mortar elements is presented. The main improvement made to this second method comes from a fast computation of mortar elements. These two data transfer methods are compared with the simplest transfer method, which consists of a classical interpolation. After some academic examples, we present 2D forging and 3D friction stir welding applications. [less ▲]

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See detailLa construction des dispositifs de GRH territoriale: grille d’analyse et modalités du processus de traduction
Mazilli, Ingrid; Pichault, François ULg

in Management International = International Management = Gestión Internacional (2015), 19(3), 31-46

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See detailGenome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009
Calusinska, Magda; Hamilton, Christopher; Monsieurs, Pieter et al

in Biotechnology for Biofuels (2015), 8(27), 1-16

Background: Molecular hydrogen, given its pollution-free combustion, has great potential to replace fossil fuels in future transportation and energy production. However, current industrial hydrogen ... [more ▼]

Background: Molecular hydrogen, given its pollution-free combustion, has great potential to replace fossil fuels in future transportation and energy production. However, current industrial hydrogen production processes, such as steam reforming of methane, contribute significantly to the greenhouse effect. Therefore alternative methods, in particular the use of fermentative microorganisms, have attracted scientific interest in recent years. However the low overall yield obtained is a major challenge in biological H2 production. Thus, a thorough and detailed understanding of the relationships between genome content, gene expression patterns, pathway utilisation and metabolite synthesis is required to optimise the yield of biohydrogen production pathways. Results: In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. During the fixed pH 5.2 fermentation, by contrast, the expression levels for the [FeFe] hydrogenase coding genes were higher than during the unregulated-pH fermentation, while the nitrogenase transcripts were less abundant. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase. Conclusions: This work, contributing to the field of dark fermentative hydrogen production, provides a multidisciplinary approach for the investigation of the processes involved in the molecular H2 metabolism of clostridia. In addition, it lays the groundwork for further optimisation of biohydrogen production pathways based on genetic engineering techniques. [less ▲]

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See detailDéveloppements récents de l'assurance construction en droit européen
Kohl, Benoît ULg

in Revue trimestrielle de droit immobilier (2015), 1

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See detailSoluble factors regulated by epithelial-mesenchymal transition mediate tumour angiogenesis and myeloid cell recruitment.
Suarez-Carmona, Meggy ULg; Bourcy, Morgane ULg; LESAGE, J et al

in Journal of Pathology (The) (2015)

Epithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been ... [more ▼]

Epithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been extensively studied, the impact of EMT on the crosstalk between tumor cells and the tumor microenvironment remains elusive. We aimed to identify EMT-regulated soluble factors that facilitate the recruitment of host cells in the tumor. Our findings indicate that EMT phenotypes relate to the induction of a panel of secreted mediators, namely IL-8, IL-6, sICAM-1, PAI-1 and GM-CSF, and implicate the EMT-transcription factor Snail as a regulator of this process. We further show that EMT-derived soluble factors are pro-angiogenic in vivo (in the mouse ear sponge assay), ex vivo (in the rat aortic ring assay) and in vitro (in a chemotaxis assay). Additionally, conditioned medium from EMT-positive cells stimulates the recruitment of myeloid cells. In a bank of 40 triple-negative breast cancers, tumors presenting features of EMT were significantly more angiogenic and infiltrated by a higher quantity of myeloid cells compared to tumors with little or no EMT. Taken together, our results show that EMT programs trigger the expression of soluble mediators in cancer cells that stimulate angiogenesis and recruit myeloid cells in vivo, which might in turn favor cancer spread. [less ▲]

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See detailHypothalamic Expression Of Oestrogen Receptor Alpha And Androgen Receptor Is Sex, Age And Region Dependent In Mice.
Brock, Olivier; Mees, Christelle De; Bakker, Julie ULg

in Journal of neuroendocrinology (2015)

Sex steroid hormones act on developing neural circuits regulating the hypothalamic-pituitary-gonadal axis and are involved in hormone-sensitive behaviours. These hormones act mainly via nuclear receptors ... [more ▼]

Sex steroid hormones act on developing neural circuits regulating the hypothalamic-pituitary-gonadal axis and are involved in hormone-sensitive behaviours. These hormones act mainly via nuclear receptors, i.e. oestrogen receptor-alpha (ERalpha) and androgen receptor (AR). By using immunohistochemistry, we analysed the expression level of ERalpha and AR throughout perinatal life [at embryonic (E) day 19 and postnatal (P) days 5-15-25] and in adulthood in several hypothalamic nuclei controlling reproduction in both wild-type (WT) and aromatase knockout (ArKO) (which cannot convert testosterone into oestradiol) mice to determine whether there are sex differences in hypothalamic ERalpha and AR expression and if so, whether these are established by oestradiol action. As early as E19, ERalpha immunoreactivity (-ir) was observed at same expression levels in both sexes in the anteroventral periventricular nucleus (AVPv), the medial preoptic area (MPOA), the bed nucleus of the stria terminalis (BnST), the ventrolateral part of the ventromedial hypothalamic nucleus (VMHvl) and the arcuate nucleus (ARC). Sex differences (female > male) in ERalpha-ir were only observed during the prepubertal period in the BnST (P5 to P25) and the MPOA (P15), but also in adulthood in these two brain regions. Sex differences in AR-ir (male > female) were observed at P5 in the AVPv and ARC, and at P25 in the MPOA and ARC as well as in adulthood in all hypothalamic regions analysed. In adulthood, gonadectomy and hormonal treatment (oestradiol or dihydrotestosterone) also strongly modulated ERalpha-ir and AR, respectively. Taken together, sex differences in ERalpha-ir and AR-ir were observed in all hypothalamic regions analysed, but most likely do not reflect oestradiol actions since ArKO mice of both sexes showed very similar expression levels as WT mice throughout perinatal development. This article is protected by copyright. All rights reserved. [less ▲]

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See detailLimited evidence for trans-generational effects of maternal dietary supplementation with ?-3 fatty acids on immunity in broiler chickens
Koppenol, A.; Delezie, E.; Parmentier, H. K. et al

in Veterinary Journal (2015), 203(2), 244-249

The aim of the present study was to investigate whether the immune response of broiler chickens is modulated by including different omega-3 (?-3) polyunsaturated fatty acids (PUFAs) in the maternal diet ... [more ▼]

The aim of the present study was to investigate whether the immune response of broiler chickens is modulated by including different omega-3 (?-3) polyunsaturated fatty acids (PUFAs) in the maternal diet. Broiler breeder hens (n = 120 birds per group) were fed one of four diets, differing in the ratios of n-6:n-3 PUFAs and eicosapentaenoic acid (EPA):docosahexaenoic acid (DHA). At 28 weeks of age, the eggs produced were incubated to obtain 720 chicks (n = 180 per group). All broiler chicks were fed a control diet and were vaccinated against Newcastle disease virus (NDV). Blood samples were taken at different time points after immunisation with human serum albumin (HuSA) in Freund's adjuvant to determine the acute phase response, antibody response and cytokine production. Addition of EPA to the maternal diet was associated with greater ovotransferrin concentrations post-immunisation, compared to other groups. Altering the ratios of n-6:n-3 PUFA or EPA:DHA in the maternal diet did not affect the offspring in terms of production of caeruloplasmin, ?1-acid glycoprotein, interleukin (IL)-1?, IL-6, IL-12 or tumour necrosis factor (TNF)-?. Dietary manipulation of the maternal diet did not influence the specific antibody response to HuSA or NDV, nor did it alter the levels of natural antibody binding to keyhole limpet haemocyanin in the offspring. Thus, maternal supplementation with n-3 PUFAs played a minor role in perinatal programming of the immune response of broiler chickens. � 2014 Elsevier Ltd. [less ▲]

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See detailMicrowave-assisted thermochemical and primary hydrolytic conversions of lignocellulosic resources: a review
Richel, Aurore ULg; Jacquet, Nicolas ULg

in Biomass Conversion and Biorefinery (2015), 5(1), 115-124

Faced with the inevitable depletion of fossil resources, agricultural productions have rapidly emerged as promising renewable alternatives. Particularly, the conversion of lignocellulosic materials has ... [more ▼]

Faced with the inevitable depletion of fossil resources, agricultural productions have rapidly emerged as promising renewable alternatives. Particularly, the conversion of lignocellulosic materials has nowadays opened new vistas for the production of energy, biofuels and chemicals. In this literature review, microwave technology is described as an original heating source either for the thermochemical conversions (at temperatures up to 400°C) of lignocellulose into biofuels or the pretreatment (below 400°C) and further hydrolysis of lignocellulose into bioethanol and other valuable chemicals. Advantages of microwave approaches include a commonly observed acceleration in reaction rate and improved selectivities and yields. [less ▲]

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