References of "Scientific journals"      in Complete repository Arts & humanities   Archaeology   Art & art history   Classical & oriental studies   History   Languages & linguistics   Literature   Performing arts   Philosophy & ethics   Religion & theology   Multidisciplinary, general & others Business & economic sciences   Accounting & auditing   Production, distribution & supply chain management   Finance   General management & organizational theory   Human resources management   Management information systems   Marketing   Strategy & innovation   Quantitative methods in economics & management   General economics & history of economic thought   International economics   Macroeconomics & monetary economics   Microeconomics   Economic systems & public economics   Social economics   Special economic topics (health, labor, transportation…)   Multidisciplinary, general & others Engineering, computing & technology   Aerospace & aeronautics engineering   Architecture   Chemical engineering   Civil engineering   Computer science   Electrical & electronics engineering   Energy   Geological, petroleum & mining engineering   Materials science & engineering   Mechanical engineering   Multidisciplinary, general & others Human health sciences   Alternative medicine   Anesthesia & intensive care   Cardiovascular & respiratory systems   Dentistry & oral medicine   Dermatology   Endocrinology, metabolism & nutrition   Forensic medicine   Gastroenterology & hepatology   General & internal medicine   Geriatrics   Hematology   Immunology & infectious disease   Laboratory medicine & medical technology   Neurology   Oncology   Ophthalmology   Orthopedics, rehabilitation & sports medicine   Otolaryngology   Pediatrics   Pharmacy, pharmacology & toxicology   Psychiatry   Public health, health care sciences & services   Radiology, nuclear medicine & imaging   Reproductive medicine (gynecology, andrology, obstetrics)   Rheumatology   Surgery   Urology & nephrology   Multidisciplinary, general & others Law, criminology & political science   Civil law   Criminal law & procedure   Criminology   Economic & commercial law   European & international law   Judicial law   Metalaw, Roman law, history of law & comparative law   Political science, public administration & international relations   Public law   Social law   Tax law   Multidisciplinary, general & others Life sciences   Agriculture & agronomy   Anatomy (cytology, histology, embryology...) & physiology   Animal production & animal husbandry   Aquatic sciences & oceanology   Biochemistry, biophysics & molecular biology   Biotechnology   Entomology & pest control   Environmental sciences & ecology   Food science   Genetics & genetic processes   Microbiology   Phytobiology (plant sciences, forestry, mycology...)   Veterinary medicine & animal health   Zoology   Multidisciplinary, general & others Physical, chemical, mathematical & earth Sciences   Chemistry   Earth sciences & physical geography   Mathematics   Physics   Space science, astronomy & astrophysics   Multidisciplinary, general & others Social & behavioral sciences, psychology   Animal psychology, ethology & psychobiology   Anthropology   Communication & mass media   Education & instruction   Human geography & demography   Library & information sciences   Neurosciences & behavior   Regional & inter-regional studies   Social work & social policy   Sociology & social sciences   Social, industrial & organizational psychology   Theoretical & cognitive psychology   Treatment & clinical psychology   Multidisciplinary, general & others     Showing results 1101 to 1200 of 55661     7 8 9 10 11 12 13 14 15 16 17     Health capability of family caregivers: how different factors interrelate and their respective contributions using a Bayesian approachBucki, Barbara; Spitz, Elisabeth; Etienne, Anne-Marie et alin BMC Public Health (2016), 16(1), 364Background: The lifestyles of family caregivers pose risks to their physical, mental and social health. The capability to stay healthy may be protective in the context of poor socioeconomic conditions and ... [more ▼]Background: The lifestyles of family caregivers pose risks to their physical, mental and social health. The capability to stay healthy may be protective in the context of poor socioeconomic conditions and risk behaviours, but the interrelations between its aspects and their respective influences remain unclear. The aim of this study was to evaluate the interrelations between the factors comprising health capability of family caregivers (HCFC) and the respective contributions of its components. Methods: All stroke patients admitted to all hospitals in Luxembourg were identified by the ‘Inspection Générale de la Sécurité Sociale’ using the national database system for care expenditure reimbursement, and asked to designate the main person caring for them. Sixty-two caregivers (mean age 59.3 years; 40 women and 22 men) responded face to face, to a questionnaire including 20 items measuring eight aspects of health capability (physical functioning, psychological functioning, lifestyle value, self-efficacy towards the use of health services, family support, social capital, material conditions/sense of security, and satisfaction with the interactions with health services). Using a Bayesian approach, significance values were estimated by comparing the test values to the posterior distribution of the parameters. Structural equation modelling with standard deviations was applied. Results: Female family caregivers had lower scores than men in physical and psychological functioning. Family caregivers with the lowest incomes had the least lifestyle value, social capital and material conditions/security. Self-efficacy towards health services increased with age. The material conditions/sense of security factor was positively correlated with almost all the others. The items that impacted health capability factors the most were - for physical functioning – atigue, and - for family support - feeling abandoned by the family. Conclusions: During the chronic phase, relationships between risk behaviours can help guide social and health decision-makers to determine their priorities in improving the lives of family caregivers. Enhancing health capability involves implementing programs that relieve family caregivers physically, and foster family networking around the person being cared for. Special attention should also be paid to the socially disadvantaged in order to fight inequalities in health capability. [less ▲]Detailed reference viewed: 16 (2 ULg) Abelian bordered factors and periodicityCharlier, Emilie ; Harju, Tero; Puzynina, Svetlana et alin European Journal of Combinatorics (2016), 51A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only ... [more ▼]A finite word u is said to be bordered if u has a proper prefix which is also a suffix of u, and unbordered otherwise. Ehrenfeucht and Silberger proved that an infinite word is purely periodic if and only if it contains only finitely many unbordered factors. We are interested in abelian and weak abelian analogues of this result; namely, we investigate the following question(s): Let w be an infinite word such that all sufficiently long factors are (weakly) abelian bordered; is w (weakly) abelian periodic? In the process we answer a question of Avgustinovich et al. concerning the abelian critical factorization theorem. [less ▲]Detailed reference viewed: 38 (8 ULg) Revealing the formation of copper nanoparticles from a homogeneous solid precursor by electron microscopyVan den Berg, Roy; Elkjaer, Christian; Gommes, Cédric et alin Journal of the American Chemical Society (2016)The understanding of processes leading to the formation of nanometer-sized particles is important for tailoring of their size, shape and location. The growth mechanisms and kinetics of nanoparticles from ... [more ▼]The understanding of processes leading to the formation of nanometer-sized particles is important for tailoring of their size, shape and location. The growth mechanisms and kinetics of nanoparticles from solid precursors are, however, often poorly described. Here we employ transmission electron microscopy (TEM) to examine the formation of copper nanoparticles on a silica support during the reduction by H2 of homogeneous copper phyllosilicate plates, as a prototype precursor for a co-precipitated catalyst. Specifically, time-lapsed TEM image series acquired of the material during the reduction provide a direct visualization of the growth dynamics of an ensemble of individual nanoparticles and enable a quantitative evaluation of the nucleation and growth of the nanoparticles. This quantitative information is compared with kinetic models and found to be best described by a nucleation-and-growth scenario involving autocatalytic reduction of the copper phyllosilicate followed by diffusion-limited or reaction-limited growth of the copper nanoparticles. The plate-like structure of the precursor restricted the diffusion of copper and the autocatalytic reduction limited the probability for secondary nucleation. The combination of a uniform size of precursor particles and the autocatalytic reduction thus offers means to synthesize nanoparticles with well-defined sizes in large amounts. In this way, in situ observations made by electron microscopy provide mechanistic and kinetic insights into the formation of metallic nanoparticles, essential for the rational design of nanomaterials. [less ▲]Detailed reference viewed: 146 (12 ULg) Compte-rendu de Pierre-Jean Renaudie, Husserl et les catégories. Langage, pensée et perception, Paris, Vrin, 2015Leclercq, Bruno in Philosophiques (2016), 43(1), 149-153Detailed reference viewed: 20 (0 ULg) Recording and Reading Alchemy and Art-Technology in Medieval and Premodern German Recipe CollectionsNeven, Sylvie in Nuncius. Journal of the Material and Visual History of Science (2016)Detailed reference viewed: 3 (1 ULg) Clostridium difficile in foods and animals: A comprehensive reviewRodriguez Diaz, Cristina ; Taminiau, Bernard ; Van Broeck, Johan et alin Advances in Experimental Medicine and Biology (2016), 27Detailed reference viewed: 37 (1 ULg) Simultaneous multi-scale and multi-instrument observations of Saturn's aurorae during the 2013 observing campaignMelin, H.; Badman, S.; Stallard, T. et alin Icarus (2016)Detailed reference viewed: 9 (1 ULg) Effect of wheel traffic on the physical properties of a LuvisolDestain, Marie-France ; Roisin, Christian; Dalcq, Anne-Catherine et alin Geoderma (2016), 262The effects of machine traffic were assessed on a Luvisol in a temperate climate area in Belgium. Soil samples were taken from topsoil (0.07-0.25 m) and subsoil (0.35-0.50 m), on plots under long-term ... [more ▼]The effects of machine traffic were assessed on a Luvisol in a temperate climate area in Belgium. Soil samples were taken from topsoil (0.07-0.25 m) and subsoil (0.35-0.50 m), on plots under long-term reduced tillage (RT) and conventional tillage (CT). Cone index (CI), bulk density (BD) and precompression stress (Pc) were chosen as indicators of mechanical strength. Mercury intrusion porosimetry was used to characterize the soil microporosity structure. It was presented in two forms: (i) cumulative pore volume vs. equivalent pore radius r, from which four classes of porosity were defined: r < 0.2 μm, 0.2 ≤ r < 9 µm, 9 ≤ r < 73 µm and r ≥ 73 μm; (ii) pore-size distribution (PSDs). In the reference situation where there had been no recent passage of machines, the voids with 0.2 ≤ r < 9 µm were the most important class in RT topsoil. The voids with r ≥ 73 µm represented the main porosity class in the topsoil of CT. In the subsoil, for both tillage systems, the porosity was almost equally distributed between voids with 0.2 ≤ r < 9 µm and voids with r greater than 9 µm. Machine traffic was carried out when the soil water content was close to the optimum Proctor. Although unfavourable, these wet conditions often occur during the beet harvesting period in Belgium. The highest modifications in soil structure (increase in BD and Pc, reduction of macroporosity r ≥ 73 μm) were observed in the topsoil of CT. More limited modifications were noticed in the soil structure of RT topsoil and subsoil layers but these latter are problematic in that the soil would no longer be loosened by subsequent tillage. These modifications could lead to soil consolidation as a result of wheel traffic year after year. [less ▲]Detailed reference viewed: 56 (30 ULg) Gemtuzumab Ozogamicin Versus Best Supportive Care in Older Patients With Newly Diagnosed Acute Myeloid Leukemia Unsuitable for Intensive Chemotherapy: Results of the Randomized Phase III EORTC-GIMEMA AML-19 TrialAmadori, Sergio; Suciu, Stefan; Selleslag, Dominik et alin Journal of Clinical Oncology (2016)Purpose To compare single-agent gemtuzumab ozogamicin (GO) with best supportive care (BSC) including hydroxyurea as first-line therapy in older patients with acute myeloid leukemia unsuitable for ... [more ▼]Purpose To compare single-agent gemtuzumab ozogamicin (GO) with best supportive care (BSC) including hydroxyurea as first-line therapy in older patients with acute myeloid leukemia unsuitable for intensive chemotherapy. Patients and Methods In this trial, patients at least 61 years old were centrally randomized (1:1) to receive either a single induction course of GO (6 mg/m2 on day 1 and 3 mg/m2 on day 8) or BSC. Patients who did not progress after GO induction could receive up to eight monthly infusions of the immunoconjugate at 2 mg/m2. Randomization was stratified by age, WHO performance score, CD33 expression status, and center. The primary end point was overall survival (OS) by intention-to-treat analysis. Results A total of 237 patients were randomly assigned (118 to GO and 119 to BSC). The median OS was 4.9 months (95% CI, 4.2 to 6.8 months) in the GO group and 3.6 months (95% CI, 2.6 to 4.2 months) in the BSC group (hazard ratio, 0.69; 95% CI, 0.53 to 0.90; P = .005); the 1-year OS rate was 24.3% with GO and 9.7% with BSC. The OS benefit with GO was consistent across most subgroups, and was especially apparent in patients with high CD33 expression status, in those with favorable/intermediate cytogenetic risk profile, and in women. Overall, complete remission (CR [complete remission] + CRi [CR with incomplete recovery of peripheral blood counts]) occurred in 30 of 111 (27%) GO recipients. The rates of serious adverse events (AEs) were similar in the two groups, and no excess mortality from AEs was observed with GO. Conclusion First-line monotherapy with low-dose GO, as compared with BSC, significantly improved OS in older patients with acute myeloid leukemia who were ineligible for intensive chemotherapy. No unexpected AEs were identified and toxicity was manageable. [less ▲]Detailed reference viewed: 18 (0 ULg) Formulation of a 1D finite element of heat exchanger for accurate modelling of the grouting behaviour: Application to cyclic thermal loadingCerfontaine, Benjamin ; Radioti, Georgia ; Collin, Frédéric et alin Renewable Energy : An International Journal (2016), 96This paper presents a comprehensive formulation of a finite element for the modelling of borehole heat exchangers. This work focuses on the accurate modelling of the grouting and the field of temperature ... [more ▼]This paper presents a comprehensive formulation of a finite element for the modelling of borehole heat exchangers. This work focuses on the accurate modelling of the grouting and the field of temperature near a single borehole. Therefore the grouting of the BHE is explicitly modelled. The purpose of this work is to provide tools necessary to the further modelling of thermo-mechanical couplings. The finite element discretises the classical governing equation of advection-diffusion of heat within a 1D pipe connected to ground nodes. Petrov-Galerkin weighting functions are used to avoid numerical disturbances. The formulation is able to capture highly transient and steady-state phenomena. The proposed finite element is validated with respect to analytical solutions. An example consisting of a 100 m depth U-pipe is finally simulated. A first continuous heating simulation highlights the nonsymmetric distribution of temperature inside and near the borehole. An estimation of the error on the results as a function of the resolution parameters is also carried out. Finally simulations of cyclic thermal loading exhibit the need to take into account all daily variations if the grouting behaviour must be modelled. This is true especially in case of freeze-thaw damaging risk. [less ▲]Detailed reference viewed: 38 (6 ULg) « La Théorie critique n’a aucun rapport avec la société. » Kluge, Adorno et l’indomptable Leni PeickertHamers, Jérémy ; Cormann, Grégory in Tijdschrift voor Filosofie (2016), 78In Artists Under the Big Top: Perplexed (1968), Alexander Kluge’s second feature film, as well as in its short sequel The Indomitable Leni Peickert (1969), the filmmaker reflects about the possibility to ... [more ▼]In Artists Under the Big Top: Perplexed (1968), Alexander Kluge’s second feature film, as well as in its short sequel The Indomitable Leni Peickert (1969), the filmmaker reflects about the possibility to create a « reformed circus » which aims at reintroducing some sensuality in the spectator’s experience. Taking this project as well as its central figure, the apparently passive elephant, as a metaphorical starting point, this article reconstitutes a virtual dialogue between the filmmaker and his friend and former master Theodor W. Adorno around the issue of communicability and effectiveness of Critical Theory. Crossing several text fragments by the philosopher (Dialectic of Enlightenment, Resignation and Education After Auschwitz a.o.) with film excerpts, short stories and interviews by Kluge, this dialogue made of real exchanges, as well as direct and indirect quotes of Adorno in Kluge’s films – considered here to be belated joint projects –, finally tries to give an answer to following crucial question: how can Adorno’s critical reflection remain politically, that is socially effective. [less ▲]Detailed reference viewed: 53 (2 ULg) Cytogenetic Studies of Rwandan Pediatric Patients Presenting with Global Developmental Delay, Intellectual Disability and/or Multiple Congenital Anomalies.Uwineza, Annette; Hitayezu, Janvier; JAMAR, Mauricette et alin Journal of tropical pediatrics (2016)Global developmental delay (GDD) is defined as a significant delay in two or more developmental domains: gross or fine motor, speech/language, cognitive, social/personal and activities of daily living ... [more ▼]Global developmental delay (GDD) is defined as a significant delay in two or more developmental domains: gross or fine motor, speech/language, cognitive, social/personal and activities of daily living. Many of these children will go on to be diagnosed with intellectual disability (ID), which is most commonly defined as having an IQ <75 in addition to impairment in adaptive functioning. Cytogenetic studies have been performed in 664 Rwandan pediatric patients presenting GDD/ID and/or multiple congenital abnormalities (MCA). Karyotype analysis was performed in all patients and revealed 260 chromosomal abnormalities. The most frequent chromosomal abnormality was Down syndrome and then Edward syndrome and Patau syndrome. Other identified chromosomal abnormalities included 47,XX,+del(9)(q11), 46,XY,del(13)(q34) and 46,XX,der(22)t(10;22)(p10;p10)mat. In conclusion, our results highlight the high frequency of cytogenetically detectable abnormalities in this series, with implications for the burden on the healthcare. This study demonstrates the importance of cytogenetic analysis in patients with GDD/ID and MCA. [less ▲]Detailed reference viewed: 9 (4 ULg) Samaras and seedlings of Acer pseudoplatanus are potential sources of hypoglycin A intoxication in atypical myopathy without necessarily inducing clinical signsBaise, Etienne ; Habyarimana, Jean ; Amory, Hélène et alin Equine Veterinary Journal (2016)Detailed reference viewed: 79 (33 ULg) Integrated multi-sensor system for parallel in-situ monitoring of cell nutrients, metabolites, cell density and pH in biotechnological processesMross, Stefan; Zimmermann, Tom; Winkin, N et alin Sensors and Actuators. B, Chemical (2016)We report on a multi-sensor system for the parallel in-situ monitoring of cell nutrients, metabolites, cell density and pH in biotechnological processes. The fabrication process of the sensor chip, based ... [more ▼]We report on a multi-sensor system for the parallel in-situ monitoring of cell nutrients, metabolites, cell density and pH in biotechnological processes. The fabrication process of the sensor chip, based on a CMOS process ﬂow, is suitable for integration into standard processes and mass production of the system. The integration of enzyme sensors for wide concentration ranges, an impedance-based sensor and a pH sensor on one silicon chip for in-situ applications in bioreactors, fabricated in a CMOS-based process ﬂow, is demonstrated for the ﬁrst time to our best knowledge. Measurements of glucose and lactate concentrations in a wide range are shown with linear ranges up to 600 mMand 900 mM, respectively. Cell density is determined via conductivity changes of a cell suspension and measurements are performed up to 15 g/l dry cell weight. The pH sensor is based on C-V measurements at an electrolyte-insulator semiconductor structure and able to measure pHin a range from pH3 to pH12. All sensors are successfully employed in the monitoring of cultures of Saccharomyces cerevisiae or Lactobacillus acidophilus. [less ▲]Detailed reference viewed: 3 (2 ULg) Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBWX7 common interactor.Daakour, Sarah ; Hajingabo, Leon Juvenal; Kerselidou, Despoina et alin BMC Cancer (2016), 16(1), 335BACKGROUND: Perturbed genotypes in cancer can now be identified by whole genome sequencing of large number of diverse tumor samples, and observed gene mutations can be used for prognosis and ... [more ▼]BACKGROUND: Perturbed genotypes in cancer can now be identified by whole genome sequencing of large number of diverse tumor samples, and observed gene mutations can be used for prognosis and classification of cancer subtypes. Although mutations in a few causative genes are directly linked to key signaling pathways perturbation, a global understanding of how known cancer genes drive oncogenesis in human is difficult to assess. METHODS: We collected available information about mutated genes in Acute Lymphoblastic Leukemia (ALL). Validated human protein interactions (PPI) were collected from IntAct, HPRD and BioGRID interactomics databases, or obtained using yeast two-hybrid screening assay. RESULTS: We have mapped interconnections between 116 cancer census gene products associated with ALL. Combining protein-protein interactions data and cancer-specific gene mutations information, we observed that 63 ALL-gene products are interconnected and identified 37 human proteins interacting with at least 2 ALL-gene products. We highlighted exclusive and coexistence genetic alterations in key signaling pathways including the PI3K/AKT and the NOTCH pathways. We then used different cell lines and reporter assay systems to validate the involvement of EXT1 in the Notch pathway. CONCLUSION: We propose that novel ALL-gene candidates can be identified based on their functional association with well-known cancer genes. We identified EXT1, a gene not previously linked to ALL via mutations, as a common interactor of NOTCH1 and FBXW7 regulating the NOTCH pathway in an FBXW7-dependend manner. [less ▲]Detailed reference viewed: 56 (6 ULg) Can breeder reproductive status, performance and egg quality be enhanced by supplementation and transition of n-3 fatty acids?Delezie, E.; Koppenol, A.; Buyse, J. et alin Journal of Animal Physiology and Animal Nutrition (2016)Summary: The aim of this experiment was to investigate the effect of n-3 fatty acid (FA) supplemented diets on breeder performance, productivity and egg quality. Breeders (n = 480) were fed the ... [more ▼]Summary: The aim of this experiment was to investigate the effect of n-3 fatty acid (FA) supplemented diets on breeder performance, productivity and egg quality. Breeders (n = 480) were fed the supplemented diet from 18 weeks onwards; the inclusion level of n-3 FA was increased from 1.5% to 3.0% from 34 weeks of age onwards until 48 weeks of age. Ross-308 broiler breeders (n = 480) were fed one of four different diets: a basal diet rich in n-6 FA (control diet) or one of three diets rich in n-3 FA. For the n-3 FA diets, eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) were fed to the broiler breeders at different ratios formulated to obtain EPA/DHA ratios of 1/1, 1/2 or 2/1. Differences in performance, reproduction and egg quality parameters due to n-3 supplementation were noted more for the 1.5% followed by the 3.0% fed broilers than their 1.5% supplemented counterparts. Egg weight (p < 0.001) and egg mass (p = 0.003) were significantly lower and feed conversion (p = 0.008) significantly higher for the n-3 FA (at 3.0% inclusion level) fed broilers compared to the control group. For the EPA- and DHA-fed breeders, a higher proportional abdominal fat percentage (p = 0.025) and proportional albumen weight (%) (p = 0.041) were found respectively. Dietary treatments did not affect reproduction. It can be concluded that the results of the present experiment indicate no significant differences between treatments at 1.5% inclusion levels. However, increasing this level to 3.0% is not recommended due to the rather negative effects on the measured parameters. It should be further investigated whether these adverse effects were obtained due to (i) the higher supplementation level, (ii) combining a supplementation level of 1.5% with 3% or (iii) the duration of supplementation. © 2016 Blackwell Verlag GmbH. [less ▲]Detailed reference viewed: 17 (1 ULg) Fossil Biogeography: A new model to infer dispersal, extinction and sampling from paleontological dataSilvestro, Daniele; Zizka, Alexander; Bacon, Christine D. et alin Philosophical Transactions : Biological Sciences (2016)Detailed reference viewed: 78 (3 ULg) Influences of the aqueous synthesis way and organosilane nature on the physico-chemical properties of porous aluminaClaude, Vincent ; Vilaseca, Miriam; Tatton, Andrew et alin European Journal of Inorganic Chemistry (2016), 2016(11), 1678-1689The aqueous sol-gel synthesis of γ-Al2O3 modified with different silicon precursors {trimethoxysilane, triethoxysilane and N-[3-(trimethoxysilyl)propyl]ethylene diamine} has been investigated. Two ... [more ▼]The aqueous sol-gel synthesis of γ-Al2O3 modified with different silicon precursors {trimethoxysilane, triethoxysilane and N-[3-(trimethoxysilyl)propyl]ethylene diamine} has been investigated. Two parameters have been studied: the silicon addition step and the type of silicon alkoxide. A first observation was that adding a silicon alkoxide either before or just after the precipitation step influences both the crystallinity and structure, whereas adding the alkoxide after a long agitation time only slightly modifies the support properties. It was also highlighted that due to their higher reactivity, silicon precursors with methoxy groups interact more strongly with the bulk alumina than the silicon precursors with ethoxy groups. This difference of Si location in the alumina structure permitted not only their resistance toward phase transition to be increased at high temperature, but also to stabilize them more efficiently against sintering under normal and steam conditions. Among the different silicon alkoxides tested, samples prepared with N-[3-(trimethoxysilyl)propyl]ethylene diamine showed very unusual properties. The alumina supports modified with this precursor showed grain-shaped nanocrystallites, whereas all other samples were platelet-like. As a result, this sample presented a very narrow pore diameter distribution of around 5 nm and a remarkably high specific surface area (530 m2/g). [less ▲]Detailed reference viewed: 11 (0 ULg) Prevention of Incisional Hernias by Prophylactic Mesh-augmented Reinforcement of Midline Laparotomies for Abdominal Aortic Aneurysm Treatment: A Randomized Controlled Trial.Muysoms, Filip E.; DETRY, Olivier ; Vierendeels, Tijl et alin Annals of surgery (2016), 263(4), 638-645BACKGROUND: The incidence of incisional hernias after abdominal aortic aneurysm repair is high. Prophylactic mesh-augmented reinforcement during laparotomy closure has been proposed in patients at high ... [more ▼]BACKGROUND: The incidence of incisional hernias after abdominal aortic aneurysm repair is high. Prophylactic mesh-augmented reinforcement during laparotomy closure has been proposed in patients at high risk of incisional hernia. METHODS: A multicenter randomized trial was conducted on patients undergoing elective abdominal aortic aneurysm repair through a midline laparotomy (Clinical.Trials.gov: NCT00757133). In the study group, retromuscular mesh-augmented reinforcement was performed with a large-pore polypropylene mesh (Ultrapro, width 7.5 cm). The primary endpoint was the incidence of incisional hernias at 2-year follow-up. RESULTS: Between February 2009 and January 2013, 120 patients were recruited at 8 Belgian centers. Patients' characteristics at baseline were similar between groups. Operative and postoperative characteristics showed no difference in morbidity or mortality. The cumulative incidence of incisional hernias at 2-year follow-up after conventional closure was 28% (95% confidence interval [CI], 17%-41%) versus 0% (95% CI, 0%-6%) after mesh-augmented reinforcement (P < 0.0001; Fisher exact test). The estimated "freedom of incisional hernia" curves (Kaplan-Meier estimate) were significantly different across study arms (chi = 19.5, P < 0.0001; Mantel-Cox test). No adverse effect related to mesh-augmented reinforcement was observed, apart from an increased mean time to close the abdominal wall for mesh-augmented reinforcement compared with the control group: 46 minutes (SD, 18.6) versus 30 minutes (SD, 18.5), respectively (P < 0.001; Mann-Whitney U test). CONCLUSIONS: Prophylactic retromuscular mesh-augmented reinforcement of a midline laparotomy in patients with abdominal aortic aneurysm is safe and effectively prevents the development of incisional hernia during 2 years, with an additional mean operative time of 16 minutes. [less ▲]Detailed reference viewed: 13 (4 ULg) ON THE USE OF HIGH-THROUGHPUT SEQUENCING FOR THE STUDY OF CYANOBACTERIAL DIVERSITY IN ANTARCTIC AQUATIC MATS 1Stelmach Pessi, Igor ; De Carvalho Maalouf, Pedro; Laughinghouse, Haywood Dail et alin Journal of Phycology (2016), 52The study of Antarctic cyanobacterial diversity has been mostly limited to morphological identification and traditional molecular techniques. High-throughput sequencing (HTS) allows a much better ... [more ▼]The study of Antarctic cyanobacterial diversity has been mostly limited to morphological identification and traditional molecular techniques. High-throughput sequencing (HTS) allows a much better understanding of microbial distribution in the environment, but its application is hampered by several methodological and analytical challenges. In this work we explored the use of HTS as a tool for the study of cyanobacterial diversity in Antarctic aquatic mats. Our results highlight the importance of using artificial communities to validate the parameters of the bioinformatics procedure used to analyse natural communities, since pipeline-dependent biases had a strong effect on the observed community structures. Analysis of microbial mats from five Antarctic lakes and an aquatic biofilm from the Sub-Antarctic showed that HTS is a valuable tool for the assessment of cyanobacterial diversity. The majority of the OTUs retrieved were related to filamentous taxa such as Leptolyngbya and Phormidium, which are common genera in Antarctic lacustrine microbial mats. However, other phylotypes related to different taxa such as Geitlerinema, Pseudanabaena, Synechococcus and Chamaesiphon, Calothrix and Coleodesmium were also found. Results revealed a much higher diversity than what had been reported using traditional methods and also highlighted remarkable differences between the cyanobacterial communities of the studied lakes. The aquatic biofilm from the Sub-Antarctic had a distinct cyanobacterial community in comparison to the studied Antarctic lakes, which, in turn, displayed a salinity-dependent community-structure at the phylotype level. [less ▲]Detailed reference viewed: 25 (11 ULg) The terrestrialization process: A palaeobotanical and palynological perspective.Meyer-Berthaud, Brigitte; Servais, Thomas; Vecoli, Marco et alin Review of Palaeobotany and Palynology (2016), 224(Part 1), 1-3Detailed reference viewed: 61 (2 ULg) F-FDG PET/CT imaging in rectal cancer: relationship with the RAS mutational status.LOVINFOSSE, Pierre ; KOOPMANSCH, Benjamin ; LAMBERT, Frédéric et alin British Journal of Radiology (2016)OBJECTIVE: Treating metastatic colorectal cancer with anti-EGFR monoclonal antibodies is recommended only for patients whose tumour does not harbour mutations of KRAS or NRAS. The aim of this study was to ... [more ▼]OBJECTIVE: Treating metastatic colorectal cancer with anti-EGFR monoclonal antibodies is recommended only for patients whose tumour does not harbour mutations of KRAS or NRAS. The aim of this study was to investigate the biology of rectal cancers and specifically to evaluate the relationship between fluorine-18 fludeoxyglucose (18F-FDG) positron emission tomography (PET) intensity and heterogeneity parameters and their mutational status. METHODS: 151 patients with newly diagnosed rectal cancer were included in this retrospective study. All patients underwent a baseline 18F-FDG PET/CT within a median time interval of 27 days of tumour tissue sampling, which was performed before any treatment. Standardized uptake values (SUVs), volume-based parameters and texture analysis were studied. We retrospectively performed KRAS genotyping on codons 12, 13, 61, 117 and 146, NRAS genotyping on codons 12, 13 and 61 and BRAF on codon 600. Associations between PET/CT parameters and the mutational status were assessed using univariate and multivariate analysis. RESULTS: 83 (55%) patients had an RAS mutation: 74 KRAS and 9 NRAS, while 68 patients had no mutation (wild-type tumours). No patient had BRAF mutation. First-order features based on intensity histogram analysis were significantly associated with RAS mutations: maximum SUV (SUVmax) (p-value = 0.002), mean SUV (p-value = 0.006), skewness (p-value = 0.049), SUV standard deviation (p-value = 0.001) and SUV coefficient of variation (SUVcov) (p-value = 0.001). Both SUVcov and SUVmax showed an area under the curve of 0.65 with sensitivity of 56% and 69%, respectively, and specificity of 64% and 52%, respectively. None of the volume-based (metabolic tumour volume and total lesion glycolysis), nor local or regional textural features were associated with the presence of RAS mutations. CONCLUSION: Although rectal cancers with KRAS or NRAS mutations display a significantly higher glucose metabolism than wild-type cancers, the accuracy of the currently proposed quantitative metrics extracted from 18F-FDG PET/CT is not sufficiently high for playing a meaningful clinical role. ADVANCES IN KNOWLEDGE: RAS-mutated rectal cancers have a significantly higher glucose metabolism. However, the accuracy of 18F-FDG PET/CT quantitative metrics is not as such as the technique could play a clinical role. [less ▲]Detailed reference viewed: 33 (10 ULg) Food peptides: purification, identification and role in the metabolism.Arroume, N.; Froidevaux, R.; Kapel, R. et alin Current opinion in Food science (2016), 7Detailed reference viewed: 14 (1 ULg) Distribution of heavy and clay minerals in coastal sediment of jijel, East of Algeria: indicators of sediment sources and transport and deposition environmentsKERMANI, S.; BOUTIBA, M.; BOUTALEB, A. et alin Arabian Journal of Geosciences (2016)Detailed reference viewed: 112 (30 ULg) Response to novely and cocaine stimulant effects: lack of stability across environments in female Swiss miceNyssen, Laura ; Brabant, Christian ; Didone, Vincent et alin Psychopharmacology (2016), 233Rationale: In humans, novelty/sensation seeking is seen as a personality trait with a positive relationship with addiction vulnerability. In animal studies, one of the standard proce-dures to model ... [more ▼]Rationale: In humans, novelty/sensation seeking is seen as a personality trait with a positive relationship with addiction vulnerability. In animal studies, one of the standard proce-dures to model novelty seeking is the "response to novelty," i.e., the levels of locomotor activity in a new environment. In rodents, a positive correlation was demonstrated between the response to novelty and several effects of drugs, especially the locomotor stimulant effects of cocaine. Objectives: The present study was designed to test in mice whether the response to novelty is stable across environments and whether its relationship with the stimulant effects of cocaine is altered by environmental changes. Experiment 1 assessed the responses to novelty of the same mice in two different novel environments. Experiment 2 tested the correlation between response to novelty and acute stimulant effects of cocaine recorded in two distinct environments. Results: The results show a weak correlation only during the first 5 min of the session between the responses to novelty measured in two distinct environments. Experiment 2 demonstrates that novelty responses and stimulant effects of cocaine are positively correlated only when both behavioral responses are measured in the same environment. In contrast, the relationship between response to novelty and acute stimulant effects of cocaine is completely lost when the behavioral responses are recorded in two different environments. Conclusions: The present results question the usual interpretation of the correlation between the response to novelty and the stimulant effects of cocaine as reflecting a relationship between two underlying individual stable characteristics. [less ▲]Detailed reference viewed: 18 (4 ULg) Aerobiology over Antarctica – a new initiative for atmospheric ecologyPearce, David; Alekhina, Irina; Terauds et alin Frontiers in Microbiology (2016), 7The role of aerial dispersal in shaping patterns of biodiversity remains poorly understood, mainly due to a lack of coordinated efforts in gathering data at appropriate temporal and spatial scales. It has ... [more ▼]The role of aerial dispersal in shaping patterns of biodiversity remains poorly understood, mainly due to a lack of coordinated efforts in gathering data at appropriate temporal and spatial scales. It has been long known that the rate of dispersal to an ecosystem can significantly influence ecosystem dynamics, and that aerial transport has been identified as an important source of biological input to remote locations. With the considerable effort devoted in recent decades to understanding atmospheric circulation in the south-polar region, a unique opportunity has emerged to investigate the atmospheric ecology of Antarctica, from regional to continental scales. This concept note identifies key questions in Antarctic microbial biogeography and the need for standardized sampling and analysis protocols to address such questions. A consortium of polar aerobiologists is established to bring together researchers with a common interest in the airborne dispersion of microbes and other propagules in the Antarctic, with opportunities for comparative studies in the Arctic. [less ▲]Detailed reference viewed: 18 (4 ULg) Breast cancer in a male to female transsexual patient with a BRCA2 mutationCORMAN, Vinciane ; Potorac, Iulia ; Manto, Florence et alin Endocrine-Related Cancer (2016), 23(5), 391-397Breast cancer is rare in male patients. Certain predisposing factors, be they genetic (e.g., BRCA2 gene mutations) or hormonal (imbalance between estrogen and androgen levels), have been implicated in ... [more ▼]Breast cancer is rare in male patients. Certain predisposing factors, be they genetic (e.g., BRCA2 gene mutations) or hormonal (imbalance between estrogen and androgen levels), have been implicated in male breast cancer pathophysiology. Male-to-female (MtF) transsexualism is a condition that generally involves cross-sex hormone therapy. Anti-androgens and estrogens are used to mimic the female hormonal environment and induce the cross-sex secondary characteristics. In certain situations, the change in the hormonal milieu can be disadvantageous and favor the development of hormonedependent pathologies, such as cancer. We report a case of a MtF transgender patient who developed breast cancer after 7 years of cross-sex hormonal therapy. The patient was found to be BRCA2 positive, and suffered recurrent disease. The patient was unaware of being a member of an established BRCA2 mutation-positive kindred. This represents the first case of a BRCA2 mutation predisposing to breast cancer in a MtF transgender patient. [less ▲]Detailed reference viewed: 43 (16 ULg) The Role of Post-Translational Modifications in Hearing and DeafnessMateo Sanchez, Susana ; Freeman, Stephen ; Delacroix, Laurence et alin Cellular and Molecular Life Sciences : CMLS (2016)Post-translational modifications (PTMs) are key molecular events that modify proteins after their synthesis and modulate their ultimate functional properties by affecting their stability, localisation ... [more ▼]Post-translational modifications (PTMs) are key molecular events that modify proteins after their synthesis and modulate their ultimate functional properties by affecting their stability, localisation, interaction potential or activity. These chemical changes expand the size of the proteome adding diversity to the molecular pathways governing the biological outcome of cells. PTMs are thus crucial in regulating a variety of cellular processes such as apoptosis, proliferation and differentiation and have been shown to be instrumental during embryonic development. In addition, alterations in protein PTMs have been implicated in the pathogenesis of many human diseases, including deafness. In this review, we summarize the recent progress made in understanding the roles of PTMs during cochlear development, with particular emphasis on the enzymes driving protein phosphorylation, acetylation, methylation, glycosylation, ubiquitination and SUMOylation. We also discuss how these enzymes may contribute to hearing impairment and deafness. [less ▲]Detailed reference viewed: 17 (1 ULg) Forced Convective Drying of Wastewater Sludge with Presentation of Exergy Analysis of the DryerBennamoun, Lyes; Fraikin, Laurent ; Li, Jie et alin Chemical Engineering Communications (2016), 203The main objective is studying the fundamental aspect, by the mean of the drying kinetics, the application of forced convective drying of wastewater sludge with determination of the optimum drying ... [more ▼]The main objective is studying the fundamental aspect, by the mean of the drying kinetics, the application of forced convective drying of wastewater sludge with determination of the optimum drying conditions. The drying system is composed of two units; small samples of 2.5 g are dried in the first unit and bed of sludge weighting 250 g is dried the second unit. The experiments are performed under air temperatures varying between 80°C and 200°C. The range of the air velocity and its humidity is 1- 2 m/s and 0.005- 0.05 kgwater/kgdry air, respectively. The experiments are performed for two different sludges; activated sludge (AS) and thermalized and digested sludge (TDS). Usually, three main drying phases are observed during drying of bed of sludge. These phases are reduced to only two for small samples. Determination of the influent parameters shows that temperature of the drying air and sludge origin can profoundly influence the drying kinetic of the sludge. The exergy analysis of the two units of the drying system allows selecting 140 °C, 2 m/s, 0.05 kgwater/kgdry air as optimum drying condition with an exergy efficiency reaching 90\%. [less ▲]Detailed reference viewed: 31 (2 ULg) Current Changes in Pubertal Timing: Revised Vision in Relation with Environmental Factors Including Endocrine Disruptors.Parent, Anne-Simone ; Franssen, Delphine ; Fudvoye, Julie et alin Endocrine development (2016), 29The aim of this chapter is to revise some common views on changes in pubertal timing. This revision is based on recent epidemiological findings on the clinical indicators of pubertal timing and data on ... [more ▼]The aim of this chapter is to revise some common views on changes in pubertal timing. This revision is based on recent epidemiological findings on the clinical indicators of pubertal timing and data on environmental factor effects and underlying mechanisms. A current advancement in timing of female puberty is usually emphasized. It appears, however, that timing is also changing in males. Moreover, the changes are towards earliness for initial pubertal stages and towards lateness for final stages in both sexes. Such observations indicate the complexity of environmental influences on pubertal timing. The mechanisms of changes in pubertal timing may involve both the central neuroendocrine control and peripheral effects at tissues targeted by gonadal steroids. While sufficient energy availability is a clue to the mechanism of pubertal development, changes in the control of both energy balance and reproduction may vary under the influence of common determinants such as endocrine-disrupting chemicals (EDCs). These effects can take place right before puberty as well as much earlier, during fetal and neonatal life. Finally, environmental factors can interact with genetic factors in determining changes in pubertal timing. Therefore, the variance in pubertal timing is no longer to be considered under absolutely separate control by environmental and genetic determinants. Some recommendations are provided for evaluation of EDC impact in the management of pubertal disorders and for possible reduction of EDC exposure along the precautionary principle. [less ▲]Detailed reference viewed: 37 (11 ULg) Male-typical visuospatial functioning in gynephilic girls with gender dysphoria - organizational and activational effects of testosterone.Burke, Sarah M.; Kreukels, Baudewijntje P. C.; Cohen-Kettenis, Peggy T. et alin Journal of psychiatry & neuroscience : JPN (2016), 41(4), 150147BACKGROUND: Sex differences in performance and regional brain activity during mental rotation have been reported repeatedly and reflect organizational and activational effects of sex hormones. We ... [more ▼]BACKGROUND: Sex differences in performance and regional brain activity during mental rotation have been reported repeatedly and reflect organizational and activational effects of sex hormones. We investigated whether adolescent girls with gender dysphoria (GD), before and after 10 months of testosterone treatment, showed male-typical brain activity during a mental rotation task (MRT). METHODS: Girls with GD underwent fMRI while performing the MRT twice: when receiving medication to suppress their endogenous sex hormones before onset of testosterone treatment, and 10 months later during testosterone treatment. Two age-matched control groups participated twice as well. RESULTS: We included 21 girls with GD, 20 male controls and 21 female controls in our study. In the absence of any group differences in performance, control girls showed significantly increased activation in frontal brain areas compared with control boys (pFWE = 0.012). Girls with GD before testosterone treatment differed significantly in frontal brain activation from the control girls (pFWE = 0.034), suggesting a masculinization of brain structures associated with visuospatial cognitive functions. After 10 months of testosterone treatment, girls with GD, similar to the control boys, showed increases in brain activation in areas implicated in mental rotation. LIMITATIONS: Since all girls with GD identified as gynephilic, their resemblance in spatial cognition with the control boys, who were also gynephilic, may have been related to their shared sexual orientation rather than their shared gender identity. We did not account for menstrual cycle phase or contraceptive use in our analyses. CONCLUSION: Our findings suggest atypical sexual differentiation of the brain in natal girls with GD and provide new evidence for organizational and activational effects of testosterone on visuospatial cognitive functioning. [less ▲]Detailed reference viewed: 24 (0 ULg) La cognitivo-linguistique : pour une tentative de rapprochement de la clinique et de l'actuarielCourtain, Audrey ; Glowacz, Fabienne in Revue Internationale de Criminologie et de Police Technique et Scientifique [= RICPTS] (2016), 1Anchored in the actuarial field, risk assessment is a tool clinicians are more and more likely to use (because they can or have to) among delinquents. But, due to epistemological differences, an important ... [more ▼]Anchored in the actuarial field, risk assessment is a tool clinicians are more and more likely to use (because they can or have to) among delinquents. But, due to epistemological differences, an important debate has been occurring between the clinical evaluation and the actuarial one since the fifties. This paper suggests a media between those perspectives through cognitive linguistics: it allows a complementary articulation, while insisting on what is at stake for both clinical and actuarial developments. Linking language and cognitions, the paper introduces a reflexion that may be useful to actuarial assessment and clinical support, and may facilitate rapprochement between the latter. In this case, our linguistic approach focusses on different matters targeted by actuarial tools and whose clinical understanding may be deepened by studying the link between language and cognition; this clinical understanding bringing singularity to risk assessment. [less ▲]Detailed reference viewed: 30 (6 ULg) High Temperature Ferromagnetism in a GdAg2 MonolayerOrmaza, M.; Fernandez, L.; Ilyn, M. et alin Nano Letters (2016), 0(0), Detailed reference viewed: 19 (1 ULg) Melting processes and mantle sources of lavas on MercuryNamur, Olivier; Collinet, Max; Charlier, Bernard et alin Earth and Planetary Science Letters (2016), 439Detailed reference viewed: 16 (1 ULg) The role of diet and exercise and of glucosamine sulfate in the prevention of knee osteoarthritis: Further results from the PRevention of knee Osteoarthritis in Overweight Females (PROOF) study.Runhaar, J.; DEROISY, Rita ; van Middelkoop, M. et alin Seminars in Arthritis & Rheumatism (2016), 45(4 Suppl), 42-48Background and objectives: The PRevention of knee Osteoarthritis in Overweight Females(PROOF) study (ISRCTN42823086) described a trend for a decrease in the incidence of kneeo steoarthritis (OA) by a ... [more ▼]Background and objectives: The PRevention of knee Osteoarthritis in Overweight Females(PROOF) study (ISRCTN42823086) described a trend for a decrease in the incidence of kneeo steoarthritis (OA) by a tailored diet and exercise program (DEP)or by oral glucosamine sulfate in women at risk for the disease, using a composite clinical and/or radiological outcome. The aim of this updated post-hoc analysis was to re-assess the results according to more precise techniques and take advantage of the 2 x 2 factorial design. Methods: A total of 407 overweight( BMI > or egal 27kg/m2) women of 50–60 years of age with no diagnosis of knee OA were randomized to: (1) noDEP + placebo(Control, N = 102), (2) DEP + placebo (DEP, N = 101), (3) glucosamine sulfate + no DEP (GS, N = 102), and (4) DEP + glucosamine sulfate (DEP + GS, N = 102) and followed for 2.5 years, with standardized postero-anterior, semiflexed (MTP) view knee radiographs at baseline and end of the study. DEP consisted of a tailored low fat and/or low caloric diet and easy to implement physical activities. Glucosamine was given as oral crystalline glucosamine sulfate 1500 mg once daily ,double-blinded vs. placebo. Incident knee OA was defined as radiographic progression of >1mm minimum joint space narrowing (mJSN)in the medial tibiofemoral compartment, as previously assessed by the visual (manual) technique and by a new semi-automated method. Logistic regression analysis was used t ocalculate the odds ratio for the effect of the interventions. Results: After 2.5 years, 11.8% of control subjects developed knee OA. This incidence was decreased with glucosamine sulfate, either alone or in combination with the DEP, but not by the DEP alone. Since there was no statistical interaction between treatments, the 2x2 factorial design allowed analysis of patients receiving glucosamine sulfate (N = 204) vs. those not receiving it (N= 203), similarly for those on the DEP (N = 203) or not (N = 204). Glucosamine sulfate significantly decreased the risk of developing knee OA: odds ratio (OR) = 0.41(95% CI: 0.20–0.85, P = 0.02) by the manual JSN assessment method and OR = 0.42 (95% CI:0.20–0.92, P =0.03) by the semi-automated technique. Conversely, there was no decrease in risk with the DEP. Conclusions: Glucosamine sulfate decreased the risk of developing radiographic knee OA over 2.5 years in overweight, middle-aged women at risk, as determined by medial mJSN progression. Conversely a tailored diet and exercise program exerted no preventive effect, possibly because of the lower than expected effect on weigh tloss. [less ▲]Detailed reference viewed: 38 (7 ULg) About the Uniform Hölder Continuity of Generalized Riemann FunctionBastin, Françoise ; Nicolay, Samuel ; Simons, Laurent in Mediterranean Journal of Mathematics (2016), 13(1), 101-117In this paper, we study the uniform H\"{o}lder continuity of the generalized Riemann function~$R_{\alpha,\beta}$ (with $\alpha>1$ and $\beta>0$) defined by $R_{\alpha,\beta}(x)=\sum_{n=1}^{+\infty}\frac ... [more ▼]In this paper, we study the uniform H\"{o}lder continuity of the generalized Riemann function~R_{\alpha,\beta} (with \alpha>1 and \beta>0) defined by \[ R_{\alpha,\beta}(x)=\sum_{n=1}^{+\infty}\frac{\sin(\pi n^\beta x)}{n^\alpha},\quad x\in\mathbb{R},$ using its continuous wavelet transform. In particular, we show that the exponent we find is optimal. We also analyse the behaviour of~$R_{\alpha,\beta}$ as $\beta$ tends to infinity. [less ▲]Detailed reference viewed: 47 (15 ULg) An endogenous gibbon ape leukemia virus (GALV) identified in a rodent (Melomys sp.) from IndonesiaNiccolo, Alfano; Michaux, Johan ; Fabre, pierre-Henri et alin Journal of Virology (2016), sous presseABSTRACT Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more ... [more ▼]ABSTRACT Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more intermediate as yet unknown hosts. A highly similar virus to GALV has been identified in an Australian rodent (Melomys burtoni) after extensive screening of Australian wildlife. GALV-like viruses have also been discovered in several Southeast Asian species although screening has not been extensive and viruses discovered to date are only distantly related to GALV. We therefore screened 26 Southeast Asian rodent species for KoRV- and GALV-like sequences, using hybridization capture and high-throughput sequencing, in the attempt to identify potential GALV and KoRV hosts. Only one species, an undescribed species of Melomys from Indonesia, was positive yielding an endogenous provirus very closely related to a strain of GALV. The sequence of the critical receptor domain for GALV infection in the Indonesian Melomys sp. was consistent with the susceptibility of the species to GALV infection. The discovery of a GALV in a second Melomys species provides further evidence that Melomys may play a role in the spread of GALV-like viruses, especially since the genus is found in Indonesia, Papua New Guinea and Australia, connecting the home ranges of koalas and gibbons. IMPORTANCE The gibbon ape leukemia virus (GALV) and the koala retrovirus (KoRV) are very closely related, yet their hosts are neither closely related nor overlap geographically. Direct cross-species infection between koalas and gibbons is unlikely. Therfore, GALV and KoRV may have arisen via a cross-species transfer from an intermediate host that overlaps in range with both gibbons and koalas. Using hybridization capture and high-throughput sequencing, we have screened a wide range of rodent candidate hosts from Southeast Asia for KoRV- and GALV-like sequences. Only a Melomys species from Indonesia was positive for GALV. We report the genome sequence of this newly identified GALV, the critical domain for infection of its potential cellular receptor and its phylogenetic relationships with the other previously characterized GALVs. We hypothesize that the genus Melomys may have played a key role in cross-species transmission to other taxa. [less ▲]Detailed reference viewed: 42 (2 ULg) Apport du cathétérisme cardiaque dans le diagnostic et le traitement de la maladie coronarienne avant 40 ansMEURICE, Caroline; LEGRAND, Victor ; PIERARD, Luc in Revue Médicale de Liège (2016), 71(3), 129-136Detailed reference viewed: 34 (9 ULg) Towards sustainable food systems: the concept of agroecology and how it questions current research practices. A reviewHatt, Séverin ; Artru, Sidonie ; Brédart, David et alin Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2016), 20(Special issue 1), 215-224Introduction. Multiple environmental and socio-economic indicators show that our current agriculture and the organization of the food system need to be revised. Agroecology has been proposed as a ... [more ▼]Introduction. Multiple environmental and socio-economic indicators show that our current agriculture and the organization of the food system need to be revised. Agroecology has been proposed as a promising concept for achieving greater sustainability. This paper offers an overview and discussion of the concept based on existing literature and case studies, and explores the way it questions our current research approaches and education paradigms. Literature. In order to improve the sustainability of agriculture, the use of external and chemical inputs needs to be minimized. Agroecological farming practices seek to optimize ecological processes, thus minimizing the need for external inputs by providing an array of ecosystem services. Implementing such practices challenges the current structure of the food system, which has been criticized for its lack of social relevance and economic viability. An agroecological approach includes all stakeholders, from field to fork, in the discussion, design and development of future food systems. This inclusion of various disciplines and stakeholders raises issues about scientists and their research practices, as well as about the education of the next generation of scientists. Conclusions. Agroecology is based on the concept that agricultural practices and food systems cannot be dissociated because they belong to the same natural and socio-economic context. Clearly, agroecology is not a silver-bullet, but its principles can serve as avenues for rethinking the current approaches towards achieving greater sustainability. Adapting research approaches in line with indicators that promote inter- and transdisciplinary research is essential if progress is to be made. [less ▲]Detailed reference viewed: 92 (21 ULg) Asymmetrical magnetic fabrics in the Egersund doleritic dike swarm (SW Norway) reveal sinistral oblique rifting before the opening of the IapetusMontalbano, Salvatrice ; Bolle, Olivier ; Diot, Hervéin Journal of Structural Geology (2016), 85Detailed reference viewed: 13 (1 ULg) Decline of the Black Sea oxygen inventoryCapet, Arthur; Stanev, Emil; Beckers, Jean-Marie et alin Biogeosciences (2016), 13Detailed reference viewed: 11 (3 ULg) Feline panleukopenia virus in cerebral neurons of young and adult cats.Garigliany, Mutien-Marie ; Gilliaux, Gautier ; Jolly, Sandra et alin BMC veterinary research (2016), 12(1), 28BACKGROUND: Perinatal infections with feline panleukopenia virus (FPV) have long been known to be associated with cerebellar hypoplasia in kittens due to productive infection of dividing neuroblasts. FPV ... [more ▼]BACKGROUND: Perinatal infections with feline panleukopenia virus (FPV) have long been known to be associated with cerebellar hypoplasia in kittens due to productive infection of dividing neuroblasts. FPV, like other parvoviruses, requires dividing cells to replicate which explains the usual tropism of the virus for the digestive tract, lymphoid tissues and bone marrow in older animals. RESULTS: In this study, the necropsy and histopathological analyses of a series of 28 cats which died from parvovirus infection in 2013 were performed. Infections were confirmed by real time PCR and immunohistochemistry in several organs. Strikingly, while none of these cats showed cerebellar atrophy or cerebellar positive immunostaining, some of them, including one adult, showed a bright positive immunostaining for viral antigens in cerebral neurons (diencephalon). Furthermore, infected neurons were negative by immunostaining for p27(Kip1), a cell cycle regulatory protein, while neighboring, uninfected, neurons were positive, suggesting a possible re-entry of infected neurons into the mitotic cycle. Next-Generation Sequencing and PCR analyses showed that the virus infecting cat brains was FPV and presented a unique substitution in NS1 protein sequence. Given the role played by this protein in the control of cell cycle and apoptosis in other parvoviral species, it is tempting to hypothesize that a cause-to-effect between this NS1 mutation and the capacity of this FPV strain to infect neurons in adult cats might exist. CONCLUSIONS: This study provides the first evidence of infection of cerebral neurons by feline panleukopenia virus in cats, including an adult. A possible re-entry into the cell cycle by infected neurons has been observed. A mutation in the NS1 protein sequence of the FPV strain involved could be related to its unusual cellular tropism. Further research is needed to clarify this point. [less ▲]Detailed reference viewed: 18 (7 ULg) ORCHIDEE-CROP (v0), a new process based Agro-Land Surface Model: model description and evaluation over EuropeWu, Xiuchen; Vuichard, N.; Ciais, P. et alin Geoscientific Model Development Discussions [=GMDD] (2016), 8Detailed reference viewed: 40 (5 ULg) Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate.Carly, F.; Niu, H.; Delvigne, Frank et alin Journal of industrial microbiology & biotechnology (2016), 43(4), 517-23High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen ... [more ▼]High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen limitation in bioreactor. In the literature, there is lack of report about AOX1 promoter regulation with regard to dissolved oxygen level (DO). Therefore, in this work, chemostat cultures were performed to investigate the cell growth, metabolism and regulation of the AOX1 promoter (pAOX1) regarding co-feeding rate of optimized methanol/sorbitol mixture (methanol fraction 0.60 C-mol/C-mol) using a P. pastoris Mut+/pAOX1-lacZ strain. The oxygen transfer rates (OTR) in bioreactor were kept in the range of typical values of large bioreactor, i.e., 4-8 g/(L h) if DO equals 30 % saturation or 5-10 g/(L h) if DO nears zero. For DO >0, an increase of the carbon fed led to an increase of pAOX1 induction. By contrast, when dissolved oxygen was completely depleted, methanol accumulated, causing a 30 % decrease of pAOX1 induction. However, this decrease is more likely to be lined to methanol accumulation than to low level of dissolved oxygen (<4 % DO). Methanol/sorbitol co-feeding allowed cells to adapt to oxygen transient limitations that often occur at industrial scale with reduced effect on pAOX1 induction. The optimal feeding rate tested here was 6.6 mmol C (DCW h)(-1) at an OTR of 8.28 g O2(L h)(-1) with over fivefold pAOX1 induction (probably directly associated with target protein productivity) compared with previous work. [less ▲]Detailed reference viewed: 22 (2 ULg) Saturn’s auroral morphology and field-aligned currents during a solar wind compressionBadman, S.V.; Provan, G.; Bunce, E.J. et alin Icarus (2016)Detailed reference viewed: 27 (6 ULg) Structural Proteomics of Herpesviruses.Leroy, Baptiste; Gillet, Laurent ; Vanderplasschen, Alain et alin Viruses (2016), 8(2), Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have ... [more ▼]Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections. [less ▲]Detailed reference viewed: 24 (6 ULg) Study of intact virus-like particles of human papillomavirus by capillary electrophoresisBettonville, Virginie ; Nicol, Jérôme ; Thelen, Nicolas et alin Electrophoresis (2016), 37Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native ... [more ▼]Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native virion. Moreover, two prophylactic vaccines, Gardasil® and Cervarix®, are based on HPV-VLP L1. Analytical techniques currently used to characterize HPV-VLP, such as SDS-PAGE, Western blot, ELISA, are time-consuming and semi-quantitative. In this study, capillary electrophoresis (CE) was evaluated for the analysis of intact HPV16-VLP. The usefulness of capillary inner wall coating as well as various BGEs, pH and detergent additives were investigated. Reproducible HPV-VLP analysis in CE was achieved using poly(ethylene oxide) coated capillary and a BGE containing high salt concentration and low SDS concentration. The developed method enables HPV-VLP detection in less than 10 min (migration times RSD : 1.6 %). The identity of HPV-VLP peak was confirmed by comparison with a sample obtained from a wild-type baculovirus and with VLP-based vaccine, Gardasil®, after adjuvant dissolution. Finally, we applied the developed methodology to VLP-based vaccines, demonstrating that CE could be successfully used for vaccine quality control. [less ▲]Detailed reference viewed: 65 (22 ULg) Monitoring the freshness of fish: development of a qPCR method applied to MAP chilled whitingDehaut, Alexandre; Krzewinski, Frédéric; Grard, Thierry et alin Journal of the Science of Food and Agriculture (2016)Detailed reference viewed: 16 (1 ULg) Etude de la séquence stratigraphique des loess de la carrière de Romont (Eben-Emael, Belgique) par une campagne géotechniqueDelvoie, Simon ; Pirson, Stéphane ; Charlier, Robert et alin Notae Praehistoricae (2016), 36The Romont quarry is recently considered as a new type section for the Middle Belgium loess deposits. The site displays a nearly 10 m thick loess sequence mainly dating from the Upper Pleistocene. The ... [more ▼]The Romont quarry is recently considered as a new type section for the Middle Belgium loess deposits. The site displays a nearly 10 m thick loess sequence mainly dating from the Upper Pleistocene. The Rocourt Pedocomplex, the Humiferous Complex of Remicourt, the Malplaquet Soil and the Nagelbeek Tongued Horizon are especially well developed. Classical approaches to study the loess sequence need observable sections. Unfortunately these sections are rare in Middle Belgium, often temporary and not deep enough. To identify reference horizons, archaeologists and geologists use to resort to hand auger sampling techniques. Investigation of loess by cone penetration tests could offer an alternative to the lack of outcrops and could be used complementary to hand drilling techniques. This study focuses on a methodology to approach as a first step the loess sequence to identify the presence or not of some layers and their extension. The investigation campaign performed on the site of Romont aims to define reference horizons based on mechanical properties by comparison with an observable outcrop. Then 16 cone penetration tests have been realized behind the working face. The campaign results reveal the existence of some horizons into the loess sequence. The transition between the Humiferous Complex of Remicourt and the Rocourt Pedocomplex is the most noticeable horizon. These two pedostratigraphic units are important due to the presence of archaeological materials in Middle Belgium and borderlands. Closer to the surface the Nagelbeek Tongued Horizon and the decarbonation limit are also highlighted from results of the geotechnical campaign. [less ▲]Detailed reference viewed: 40 (9 ULg) Transatlantic Editorial: a comparison between European and North American guidelines on myocardial revascularization.Kolh, Philippe ; Kurlansky, Paul; Cremer, Jochen et alin European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (2016), 49(5), 1307-17Detailed reference viewed: 6 (0 ULg) A Simple Tiered Methodology for the Determination of Ambient Air Quality GuidelinesPIRARD, Catherine ; Brouhon, Jean-Marc; Fourmeaux, Annick et alin Clean : Soil, Air, Water (2016), 44Detailed reference viewed: 14 (4 ULg) Focus sur les astrocytes: cellules de la glie aux rôles multiples et importants dans le SNC. Le mouton est-il un excellent modèle animal ?Salouci, Moustafa ; Gabriel, Annick in Revue de Médecine Vétérinaire (2016)Les astrocytes représentent la population la plus importante de cellules gliales au sein du système nerveux central (SNC). Leur participation au fonctionnement du système nerveux central et leurs contacts ... [more ▼]Les astrocytes représentent la population la plus importante de cellules gliales au sein du système nerveux central (SNC). Leur participation au fonctionnement du système nerveux central et leurs contacts avec les éléments neuronaux impliquent pour ces cellules des rôles très importants lors du développement du système nerveux, dans le cadre de différentes fonctions vitales et également lors de processus pathologiques. En plus d’être un soutien structurel, ces cellules jouent un rôle dans le métabolisme, la production et la libération de différentes molécules, l'entretien et le remplacement des neurones ; elles assurent également des fonctions immunitaires. La similitude entre l’être humain et le mouton en ce qui concerne certains aspects développementaux et anatomiques a donné de l'importance à ce dernier en tant que modèle expérimental animal permettant de mieux comprendre les rôles joués par les astrocytes dans les processus vitaux et pathologiques. Le but de cet article est de réaliser une synthèse sur le développent et les fonctions des astrocytes et leurs interventions lors de pathologie du SNC chez l’homme et chez le mouton. Enfin, l’utilisation du mouton en tant que modèle animal dans le cadre d’études expérimentales concernant les astrocytes est abordée. [less ▲]Detailed reference viewed: 27 (6 ULg) Effects of fish predation on Posidonia oceanica amphipod assemblagesSturaro, Nicolas ; Gobert, Sylvie ; Pérez-Perera, Amanda et alin Marine Biology (2016), 163Amphipod assemblages that inhabit Posidonia oceanica seagrass meadows are potentially relevant trophic resources for ichthyofauna. However, the effects of fish predation on amphipod assemblages in this ... [more ▼]Amphipod assemblages that inhabit Posidonia oceanica seagrass meadows are potentially relevant trophic resources for ichthyofauna. However, the effects of fish predation on amphipod assemblages in this system have received little attention. To address this gap in knowledge, experimental manipulations of predation intensity (exclusion and inclusion cages) were conducted at two sites in a Mediterranean marine protected area, where different levels of fish predation were expected to occur. We found that in the absence of predatory fishes (exclusion cages), total amphipod density and biomass were higher than in uncaged areas and partially controlled cages. At the species level, Caprella acanthifera and Iphimedia minuta responded to caging with increased abundance, while in most cases different species did not exhibit differences in density or biomass between treatments. The presence of one enclosed labrid fish predator (inclusion cages) resulted in a lower density and biomass of Aora spinicornis and a lower biomass of Phtisica marina, although total amphipod density and biomass were unchanged. In the inclusion cages, a size-frequency analysis revealed that predators mainly targeted large A. spinicornis and Apherusa chiereghinii individuals. Our results suggest that predation by fish may be an important factor in controlling amphipod abundances and biomasses in P. oceanica meadows. Overall, amphipod community composition was not affected by exclusion or inclusion of fish predators. However, some significant effects at the species level point to more complex interactions between some amphipods and fish. [less ▲]Detailed reference viewed: 29 (6 ULg) Executive functions deficits after severe traumatic brain injury: The GREFEX studyAzouvi, Philippe; Vallat-Azouvi, Claire; Joseph, Pierre-Alain et alin Journal of Head Trauma Rehabilitation (2016), 31(3), 10-20Detailed reference viewed: 17 (2 ULg) SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration in 1321 N1 glioblastoma.Edimo, William S Elong; Ghosh, Somadri; Derua, Rita et alin Journal of cell science (2016)Phosphoinositides, particularly PI(3,4,5)P3, and PI(4,5)P2, are recognized by SHIP2 a member of the inositol polyphosphate 5-phosphatase family. SHIP2 dephosphorylates PI(3,4,5)P3 to form PI(3,4)P2; the ... [more ▼]Phosphoinositides, particularly PI(3,4,5)P3, and PI(4,5)P2, are recognized by SHIP2 a member of the inositol polyphosphate 5-phosphatase family. SHIP2 dephosphorylates PI(3,4,5)P3 to form PI(3,4)P2; the latter interacts with specific target proteins (e.g. lamellipodin). Although the SHIP2 preferred substrate is PI(3,4,5)P3, PI(4,5)P2 could also be dephosphorylated to PI4P. Through depletion of SHIP2 in a glioblastoma cell line 1321 N1 cells, we show that SHIP2 inhibits cell migration. In different glioblastoma cell lines and primary cultures, SHIP2 staining at the plasma membrane partly overlaps with PI(4,5)P2 immunoreactivity. PI(4,5)P2 was upregulated in SHIP2-deficient N1 cells as compared to control cells; in contrast, PI4P was very much decreased in SHIP2-deficient cells. Therefore, SHIP2 controls both PI(3,4,5)P3 and PI(4,5)P2 levels in intact cells. In N1 cells, the PI(4,5)P2 binding protein myosin-1c was identified as a new interactor of SHIP2. Regulation of PI(4,5)P2 and PI4P content by SHIP2 controls N1 cell migration through the organization of focal adhesions. Thus, our results reveal a novel role of SHIP2 in the control of PI(4,5)P2, PI4P and cell migration in PTEN-deficient glioblastoma N1 cells. [less ▲]Detailed reference viewed: 17 (5 ULg) Apoptosis of Sertoli cells after conditional ablation of murine double minute 2 (Mdm2) gene is p53-dependent and results in male sterility.Fouchecourt, S.; Livera, G.; Messiaen, S. et alin Cell death and differentiation (2016)Beside its well-documented role in carcinogenesis, the function of p53 family has been more recently revealed in development and female reproduction, but it is still poorly documented in male reproduction ... [more ▼]Beside its well-documented role in carcinogenesis, the function of p53 family has been more recently revealed in development and female reproduction, but it is still poorly documented in male reproduction. We specifically tested this possibility by ablating Mdm2, an E3 ligase that regulates p53 protein stability and transactivation function, specifically in Sertoli cells (SCs) using the AMH-Cre line and created the new SC-Mdm2-/- line. Heterozygous SC-Mdm2-/+ adult males were fertile, but SC-Mdm2-/- males were infertile and exhibited: a shorter ano-genital distance, an extra duct along the vas deferens that presents a uterus-like morphology, degenerated testes with no organized seminiferous tubules and a complete loss of differentiated germ cells. In adults, testosterone levels as well as StAR, P450c17 (Cyp17a1) and P450scc (Cyp11a1) mRNA levels decreased significantly, and both plasma LH and FSH levels increased. A detailed investigation of testicular development indicated that the phenotype arose during fetal life, with SC-Mdm2-/- testes being much smaller at birth. Interestingly, Leydig cells remained present until adulthood and fetal germ cells abnormally initiated meiosis. Inactivation of Mdm2 in SCs triggered p53 activation and apoptosis as early as 15.5 days post conception with significant increase in apoptotic SCs. Importantly, testis development occurred normally in SC-Mdm2-/- lacking p53 mice (SC-Mdm2-/-p53-/-) and accordingly, these mice were fertile indicating that the aforementioned phenotypes are entirely p53-dependent. These data not only highlight the importance of keeping p53 in check for proper testicular development and male fertility but also certify the critical role of SCs in the maintenance of meiotic repression.Cell Death and Differentiation advance online publication, 16 October 2015; doi:10.1038/cdd.2015.120. [less ▲]Detailed reference viewed: 11 (5 ULg) Synthesis of hydrophilic CuInS2/ZnS quantum dots with different polymeric shells and study of their cytotoxicity and hemocompatibilitySperanskaya, Elena Sergeevna; Sevrin, Chantal ; De Saeger, Sarah et alin ACS Applied Materials and Interfaces (2016)Detailed reference viewed: 23 (5 ULg) Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems.Bottoni, Carlo; Perilli, Mariagrazia; Marcoccia, Francesca et alin Antimicrobial Agents and Chemotherapy (2016), 60(5), 3123-6Site-directed mutagenesis of CphA indicated that prolines in the P158-P172 loop are essential for the stability and the catalytic activity of subclass B2 metallo-beta-lactamases against carbapenems. The ... [more ▼]Site-directed mutagenesis of CphA indicated that prolines in the P158-P172 loop are essential for the stability and the catalytic activity of subclass B2 metallo-beta-lactamases against carbapenems. The sequential substitution of proline led to a decrease of the catalytic efficiency of the variant compared to the wild-type (WT) enzyme but also to a higher affinity for the binding of the second zinc ion. [less ▲]Detailed reference viewed: 24 (3 ULg) Differential effects of context on psychomotor sensitization to ethanol and cocaineDidone, Vincent ; Quoilin, Caroline; Dieupart, Julie et alin Behavioural Pharmacology (2016), 27(2 & 3), 173-181Repeated drug injections lead to sensitization of their stimulant effects in mice, a phenomenon sometimes referred to as drug psychomotor sensitization. Previous studies showed that sensitization to ... [more ▼]Repeated drug injections lead to sensitization of their stimulant effects in mice, a phenomenon sometimes referred to as drug psychomotor sensitization. Previous studies showed that sensitization to cocaine is context dependent as its expression is reduced in an environment that was not paired with cocaine administration. In contrast, the effects of the test context on ethanol sensitization remain unclear. In the present study, female OF1 mice were repeatedly injected with 1.5 g/kg ethanol to test for both the effects of context novelty/familiarity and association on ethanol sensitization. A first group of mice was extensively pre-exposed to the test context before ethanol sensitization and ethanol injections were paired with the test context (familiar and paired group). A second group was not pre-exposed to the test context, but ethanol injections were paired with the test context (nonfamiliar and paired group). Finally, a third group of mice was not pre-exposed to the test context and ethanol was repeatedly injected in the home cage (unpaired group). Control groups were similarly exposed to the test context, but were injected with saline. In a second experiment, cocaine was used as a positive control. The same behavioral procedure was used, except that mice were injected with 10 mg/kg cocaine instead of ethanol. The results show a differential involvement of the test context in the sensitization to ethanol and cocaine. Cocaine sensitization is strongly context dependent and is not expressed in the unpaired group. In contrast, the expression of ethanol sensitization is independent of the context in which it was administered, but is strongly affected by the relative novelty/familiarity of the environment. Extensive pre-exposure to the test context prevented the expression of ethanol sensitization. One possible explanation is that expression of ethanol sensitization requires an arousing environment. [less ▲]Detailed reference viewed: 63 (18 ULg) Capturing the wide variety of impaired fracture healing phenotypes in Neurofibromatosis Type 1 with eight key factors: a computational study.carlier, aurelie; brems, h.; ashbourn, j.m.a. et alin Scientific Reports (2016), 7Congenital pseudarthrosis of the tibia (CPT) is a rare disease which normally presents itself during early childhood by anterolateral bowing of the tibia and spontaneous tibial fractures. Although the ... [more ▼]Congenital pseudarthrosis of the tibia (CPT) is a rare disease which normally presents itself during early childhood by anterolateral bowing of the tibia and spontaneous tibial fractures. Although the exact etiology of CPT is highly debated, 40-80% of CPT patients are carriers of a mutation in the Neurofibromatosis Type 1 (NF1) gene, which can potentially result in an altered phenotype of the skeletal cells and impaired bone healing. In this study we use a computational model of bone regeneration to examine the effect of the Nf1 mutation on bone fracture healing by altering the parameter values of eight key factors which describe the aberrant cellular behaviour of Nf1 haploinsufficient and Nf1 bi-allelically inactivated cells. We show that the computational model is able to predict the formation of a hamartoma as well as a wide variety of CPT phenotypes through different combinations of altered parameter values. A sensitivity analysis by "Design of Experiments" identified the impaired endochondral ossification process and increased infiltration of fibroblastic cells as key contributors to the degree of severity of CPT. Hence, the computational model results have added credibility to the experimental hypothesis of a genetic cause (i.e. Nf1 mutation) for CPT. [less ▲]Detailed reference viewed: 15 (4 ULg) Sex differences in partner preferences in humans and animals.Balthazart, Jacques in Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2016), 371(1688), A large number of morphological, physiological and behavioural traits are differentially expressed by males and females in all vertebrates including humans. These sex differences, sometimes, reflect the ... [more ▼]A large number of morphological, physiological and behavioural traits are differentially expressed by males and females in all vertebrates including humans. These sex differences, sometimes, reflect the different hormonal environment of the adults, but they often remain present after subjects of both sexes are placed in the same endocrine conditions following gonadectomy associated or not with hormonal replacement therapy. They are then the result of combined influences of organizational actions of sex steroids acting early during development, or genetic differences between the sexes, or epigenetic mechanisms differentially affecting males and females. Sexual partner preference is a sexually differentiated behavioural trait that is clearly controlled in animals by the same type of mechanisms. This is also probably true in humans, even if critical experiments that would be needed to obtain scientific proof of this assertion are often impossible for pragmatic or ethical reasons. Clinical, epidemiological and correlative studies provide, however, converging evidence strongly suggesting, if not demonstrating, that endocrine, genetic and epigenetic mechanisms acting during the pre- or perinatal life control human sexual orientation, i.e. homosexuality versus heterosexuality. Whether they interact with postnatal psychosexual influences remains, however, unclear at present. [less ▲]Detailed reference viewed: 42 (15 ULg) First record of the mangrove palm Nypa from the northeastern Ebro basin, Spain: with taphonomic criteria to evaluate the drifting durationMoreno-Dominguez, Rafael; Cascales-Miñana, Borja ; Ferrer, Javier et alin Geologica Acta (2016), 14(2), 101-111Detailed reference viewed: 15 (0 ULg) Crystal structure of bassetite and saléeite: new insight into autunite-group mineralsDal Bo, Fabrice ; Hatert, Frédéric ; Mees, Florias et alin European Journal of Mineralogy (2016)Detailed reference viewed: 21 (7 ULg) Accelerating chemoselective peptide bond formation using bis(2-selenylethyl)amido peptide selenoester surrogatesRaibaut, Laurent; Cargoët, Marine; Ollivier, Nathalie et alin Chemical Science (2016)Given the potential of peptide selenoesters for protein total synthesis and the paucity of methods for the synthesis of these sensitive peptide derivatives, we sought to explore the usefulness of the bis ... [more ▼]Given the potential of peptide selenoesters for protein total synthesis and the paucity of methods for the synthesis of these sensitive peptide derivatives, we sought to explore the usefulness of the bis(2-selenylethyl)amido (SeEA) group, i.e. the selenium analog of the bis(2-sulfanylethyl)amido (SEA) group, for accelerating peptide bond formation. A chemoselective exchange process operating in water was devised for converting SEA peptides into the SeEA ones. Kinetic studies show that SeEA ligation, which relies on an initial N,Se-acyl shift process, proceeds significantly faster than SEA ligation. This property enabled the design of a kinetically controlled three peptide segment assembly process based on the sequential use of SeEA and SEA ligation reactions. The method was validated by the total synthesis of hepatocyte growth factor K1 (85 AA) and biotinylated NK1 (180 AA) domains [less ▲]Detailed reference viewed: 32 (8 ULg) Unravelling the mechanisms of a protein refolding process based on the association of detergents and co-solvents.Michaux, Catherine; Roussel, Guillaume; Lopes-Rogrigues, M. et alin Journal of peptide science : an official publication of the European Peptide Society (2016)A new technique associating the detergent Sodium Dodecyl Sulphate (SDS) and an alcohol-type co-solvent has been set up, showing an unexpected efficiency to refold several types of soluble or membrane ... [more ▼]A new technique associating the detergent Sodium Dodecyl Sulphate (SDS) and an alcohol-type co-solvent has been set up, showing an unexpected efficiency to refold several types of soluble or membrane proteins. The present contribution deepens the fundamental knowledge on the phenomena underlying this process, considering the refolding of two model peptides featuring the main protein secondary structures: alpha-helix and beta-sheet. Their refolding was monitored by fluorescence and circular dichroism, and it turns out that: (i) 100% recovery of the folded structure is observed for both peptides, (ii) the highest the SDS concentration, the more co-solvent to be added to recover the peptides' native structures, (iii) a high alcohol concentration is required to alter the SDS denaturing properties, (iv) the co-solvent performance relies on its specific lipophilic-hydrophilic balanced character, (v) the size of the micelle formed by the detergent does not enter the process critical parameters, and (vi) increasing the salt concentration up to 1 M NaCl has a beneficial impact on the process efficiency. These mechanistic aspects will help us to improve the method and extend its application. Copyright (c) 2016 European Peptide Society and John Wiley & Sons, Ltd. [less ▲]Detailed reference viewed: 19 (5 ULg) Concordance between iohexol and iothalamate plasma clearanceDELANAYE, Pierre ; JOURET, François ; LE GOFF, Caroline et alin American Journal of Kidney Diseases (2016)Detailed reference viewed: 14 (2 ULg) Large-scale signatures of unconsciousness are consistent with a departure from critical dynamicsTagliazucchi, E.; Chialvo, D. R.; Siniatchkin, M. et alin Journal of the Royal Society Interface (2016), 13(114), Loss of cortical integration and changes in the dynamics of electrophysiological brain signals characterize the transition from wakefulness towards unconsciousness. In this study, we arrive at a basic ... [more ▼]Loss of cortical integration and changes in the dynamics of electrophysiological brain signals characterize the transition from wakefulness towards unconsciousness. In this study, we arrive at a basic model explaining these observations based on the theory of phase transitions in complex systems. We studied the link between spatial and temporal correlations of large-scale brain activity recorded with functional magnetic resonance imaging during wakefulness, propofol-induced sedation and loss of consciousness and during the subsequent recovery. We observed that during unconsciousness activity in frontothalamic regions exhibited a reduction of long-range temporal correlations and a departure of functional connectivity from anatomical constraints. A model of a system exhibiting a phase transition reproduced our findings, as well as the diminished sensitivity of the cortex to external perturbations during unconsciousness. This framework unifies different observations about brain activity during unconsciousness and predicts that the principles we identified are universal and independent from its causes. © 2016 The Author(s) Published by the Royal Society. All rights reserved. [less ▲]Detailed reference viewed: 12 (1 ULg) Life cycle assessment of hybrid vehicles recycling: Comparison of three business lines of dismantlingBelboom, Sandra ; Lewis, Grégory; Bareel, Pierre-François et alin Waste Management (2016), 50This paper undertakes an environmental evaluation of hybrid vehicles recycling, using industrial data from Comet Traitement SA in Belgium. Three business lines have been modelled and analysed. The first ... [more ▼]This paper undertakes an environmental evaluation of hybrid vehicles recycling, using industrial data from Comet Traitement SA in Belgium. Three business lines have been modelled and analysed. The first one is relative to the business as usual with a dismantling to recover batteries and engines followed by shredding and post shredding treatments. The second one considers, in addition, the removal of electronic control units (ECU) before shredding followed by same steps than in the first line and the last one is relative to the additional removal of big plastic parts before shredding and business as usual post shredding treatments. Results show non-significant environmental benefits when ECU or large parts of plastics are recovered before shredding. Improvements in terms of environmental benefits are lower than the uncertainty of the results. Indeed, the performing usual process for end-of-life vehicles (ELV) treatment reaches 97% of the ELV which is valorised in terms of metal and energy recoveries. Post shredding treatment units include metals, plastics and energy recovery of residues. Comet business as usual route for ELV valorisation is in accordance with the requirements of the European directive and recommendations for further improvement with dismantling of other parts (ECU or plastics) before shredding are non-relevant in this case. [less ▲]Detailed reference viewed: 34 (14 ULg) Subunit Asa1 spans all the peripheral stalk of the mitochondrial ATP synthase of the chlorophycean alga Polytomella sp.Colina-Tenorio, Lilia; Miranda-Astudillo, Hector; Cano-Estrada, Araceli et alin Biochimica et biophysica acta (2016)Mitochondrial F1FO-ATP synthase of chlorophycean algae is dimeric. It contains eight orthodox subunits (alpha, beta, gamma, delta, epsilon, OSCP, a and c) and nine atypical subunits (Asa1 to 9). These ... [more ▼]Mitochondrial F1FO-ATP synthase of chlorophycean algae is dimeric. It contains eight orthodox subunits (alpha, beta, gamma, delta, epsilon, OSCP, a and c) and nine atypical subunits (Asa1 to 9). These subunits build the peripheral stalk of the enzyme and stabilize its dimeric structure. The location of the 66.1kDa subunit Asa1 has been debated. On one hand, it was found in a transient subcomplex that contained membrane-bound subunits Asa1/Asa3/Asa5/Asa8/a (Atp6)/c (Atp9). On the other hand, Asa1 was proposed to form the bulky structure of the peripheral stalk that contacts the OSCP subunit in the F1 sector. Here, we overexpressed and purified the recombinant proteins Asa1 and OSCP and explored their interactions in vitro, using immunochemical techniques and affinity chromatography. Asa1 and OSCP interact strongly, and the carboxy-terminal half of OSCP seems to be instrumental for this association. In addition, the algal ATP synthase was partially dissociated at relatively high detergent concentrations, and an Asa1/Asa3/Asa5/Asa8/a/c10 subcomplex was identified. Furthermore, Far-Western analysis suggests an Asa1-Asa8 interaction. Based on these results, a model is proposed in which Asa1 spans the whole peripheral arm of the enzyme, from a region close to the matrix-exposed side of the mitochondrial inner membrane to the F1 region where OSCP is located. 3D models show elongated, helix-rich structures for chlorophycean Asa1 subunits. Asa1 subunit probably plays a scaffolding role in the peripheral stalk analogous to the one of subunit b in orthodox mitochondrial enzymes. [less ▲]Detailed reference viewed: 65 (5 ULg) Modular Diversification of the Locomotor System in Damselfishes (Pomacentridae)Aguilar-Medrano, Rosalia; Frederich, Bruno ; Barber, Paul H.in Journal of Morphology (2016)As fish move and interact with their aquatic environment by swimming, small morphological variations of the locomotor system can have profound implications on fitness. Damselfishes (Pomacentridae) have ... [more ▼]As fish move and interact with their aquatic environment by swimming, small morphological variations of the locomotor system can have profound implications on fitness. Damselfishes (Pomacentridae) have inhabited coral reef ecosystems for more than 50 million years. As such, habitat preferences and behavior could significantly constrain the morphology and evolvability of the locomotor system. To test this hypothesis, we used phylogenetic comparative methods on morphometric, ecological and behavioral data. While body elongation represented the primary source of variation in the locomotor system of damselfishes, results also showed a diverse suite of morphological combinations between extreme morphologies. Results show clear associations between behavior, habitat preferences, and morphology, suggesting ecological constraints on shape diversification of the locomotor system. In addition, results indicate that the three modules of the locomotor system are weakly correlated, resulting in versatile and independent characters. These results suggest that Pomacentridae is shape may result from the interaction between (1) integrated parts of morphological variation that main- tain overall swimming ability and (2) relatively independent parts of the morphology that facilitate adaptation and diversification. [less ▲]Detailed reference viewed: 13 (4 ULg) Bone disease after kidney transplantationBOUQUEGNEAU, Antoine ; Salam, Syrazah; DELANAYE, Pierre et alin Clinical Journal of the American Society of Nephrology (2016), 11(7), 1282-1296Detailed reference viewed: 28 (7 ULg) Compte rendu, La mise en scène des produits et des marques. Représentations,significations, publicsDuchesne, Caroline in Questions de Communication (2016), 28Actes d’un colloque interdisciplinaire organisé en juillet 2013 à l’Institut universitaire de technologie Nancy-Charlemagne, le volume rassemble douze contributions envisageant, en trois temps ... [more ▼]Actes d’un colloque interdisciplinaire organisé en juillet 2013 à l’Institut universitaire de technologie Nancy-Charlemagne, le volume rassemble douze contributions envisageant, en trois temps, l’évolution stratégique de la mise en scène des marques, la place symbolique occupée par celles-ci dans l’espace social et l’interpénétration grandissante des registres commerciaux et culturels présidant à leur positionnement stratégique. [less ▲]Detailed reference viewed: 57 (0 ULg) Selection of a discriminant and biorelevant in vitro dissolution test for the development of fenofibrate self-emulsifying lipid-based formulationsPestieau, Aude ; Krier, Fabrice; Brouwers, Adeline et alin European Journal of Pharmaceutical Sciences (2016)Detailed reference viewed: 16 (8 ULg) Genotyping and strain distribution of Mycobacterium avium subspecies hominissuis isolated from humans and pigs in Belgium, 2011-2013Vluggen, C; Soetaert, K; Duytschaever, L et alin Eurosurveillance (2016), 21(3), 18-25Detailed reference viewed: 10 (0 ULg) Endocrine and social regulation of adult neurogenesis in songbirds.Balthazart, Jacques ; Ball, Gregory F.in Frontiers in neuroendocrinology (2016)The identification of pronounced seasonal changes in the volume of avian song control nuclei stimulated the discovery of adult neurogenesis in songbirds as well as renewed studies in mammals including ... [more ▼]The identification of pronounced seasonal changes in the volume of avian song control nuclei stimulated the discovery of adult neurogenesis in songbirds as well as renewed studies in mammals including humans. Neurogenesis in songbirds is modulated by testosterone and other factors such as photoperiod, singing activity and social environment. Adult neurogenesis has been widely studied by labeling, with tritiated thymidine or its analog BrdU, cells duplicating their DNA in anticipation of their last mitotic division and following their fate as new neurons. New methods based on endogenous markers of cell cycling or of various stages of neuronal life have allowed for additional progress. In particular immunocytochemical visualization of the microtubule-associated protein doublecortin has provided an integrated view of neuronal replacement in the song control nucleus HVC. Multiple questions remain however concerning the specific steps in the neuronal life cycle that are modulated by various factors and the underlying cellular mechanisms. [less ▲]Detailed reference viewed: 32 (5 ULg) Experimental and Theoretical Study of the Reactivity of Gold Nanoparticles Towards Benzimidazole-2-ylidene LigandsRodríguez-Castillo, María; Lugo-Preciado, Gustavo; Laurencin, Danielle et alin Chem. Eur. J. (2016), 22Detailed reference viewed: 15 (2 ULg) IgE and mast cells in host defense against parasites and venoms.Mukai, Kaori; Tsai, Mindy; Starkl, Philipp et alin Seminars in Immunopathology (2016)IgE-dependent mast cell activation is a major effector mechanism underlying the pathology associated with allergic disorders. The most dramatic of these IgE-associated disorders is the fatal anaphylaxis ... [more ▼]IgE-dependent mast cell activation is a major effector mechanism underlying the pathology associated with allergic disorders. The most dramatic of these IgE-associated disorders is the fatal anaphylaxis which can occur in some people who have developed IgE antibodies to otherwise innocuous antigens, such as those contained in certain foods and medicines. Why would such a highly "maladaptive" immune response develop in evolution and be retained to the present day? Host defense against parasites has long been considered the only beneficial function that might be conferred by IgE and mast cells. However, recent studies have provided evidence that, in addition to participating in host resistance to certain parasites, mast cells and IgE are critical components of innate (mast cells) and adaptive (mast cells and IgE) immune responses that can enhance host defense against the toxicity of certain arthropod and animal venoms, including enhancing the survival of mice injected with such venoms. Yet, in some people, developing IgE antibodies to insect or snake venoms puts them at risk for having a potentially fatal anaphylactic reaction upon subsequent exposure to such venoms. Delineating the mechanisms underlying beneficial versus detrimental innate and adaptive immune responses associated with mast cell activation and IgE is likely to enhance our ability to identify potential therapeutic targets in such settings, not only for reducing the pathology associated with allergic disorders but perhaps also for enhancing immune protection against pathogens and animal venoms. [less ▲]Detailed reference viewed: 6 (1 ULg) Modeling heat stress under different environmental conditionsCarabano, Maria-Jesus; Logar, Betka; Bormann, Jeanne et alin Journal of Dairy Science (2016)Renewed interest in heat stress effects on livestock productivity derives from climate change, which is expected to increase temperatures and the frequency of extreme weather events. This study aimed at ... [more ▼]Renewed interest in heat stress effects on livestock productivity derives from climate change, which is expected to increase temperatures and the frequency of extreme weather events. This study aimed at evaluating the effect of temperature and humidity on milk production in highly selected dairy cattle populations across three European regions differing in climate and production systems to detect differences and similarities that can be used to optimize heat stress (HS) effect modeling. Milk, fat and protein test day data from official milk recording for years 1999 to 2010 in four Holstein populations located in the Walloon Region of Belgium (BEL), Luxembourg (LUX), Slovenia (SLO) and Southern Spain (SPA) were merged with temperature and humidity data provided by the state meteorological agencies. After merging, the number of test day records/cows per trait ranged from 686,726/49,655 in SLO to 1,982,047/136,746 in BEL. Values for the daily average and maximum temperature and humidity index (THIavg and THImax) ranges for THIavg/THImax were largest in SLO (22-74/28-84) in SLO and shortest in SPA (39-76/46-83). Change point techniques were used to determine comfort thresholds, which differed across traits and climatic regions. Milk yield showed an inverted U shaped pattern of response across the THI scale with a HS threshold around 73 THImax units. For fat and protein, thresholds were lower than for milk yield and were shifted around 6 THI units towards larger values in SPA compared with the other countries. Fat showed lower HS thresholds than protein traits in all countries. The traditional broken line model was compared to quadratic and cubic fits of the pattern of response in production to increasing heat loads. A cubic polynomial model allowing for individual variation in patterns of response and THIavg as heat load measure showed the best statistical features. Higher/lower producing animals showed less/more persistent production (quantity and quality) across the THI scale. The estimated correlations between comfort and THIavg values of 70 (which represents the upper end of the THIavg scale in BEL-LUX) were lower for BEL-LUX (0.70 - 0.80) than for SPA (0.83 - 0.85). Overall, animals producing in the more temperate climates and semi-extensive grazing systems of BEL and LUX showed HS at lower heat loads and more re-ranking across the THI scale than animals producing in the warmer climate and intensive indoor system of SPA. [less ▲]Detailed reference viewed: 95 (20 ULg) Éthologie et criminologie clinique : Debuyst avec Demaret pour une éthique de l’adaptationAdam, Christophe; Englebert, Jérôme in Cahiers de Psychologie Clinique (2016), 47Detailed reference viewed: 25 (2 ULg) Identifying the factors determining blooms of cyanobacteria in a set of shallow lakesDescy, Jean-Pierre; Leprieur, Fabien; Pirlot, Samuel et alin Ecological Informatics (2016), 34There is a strong interest in developing a capacity to predict the occurrence of cyanobacteria blooms in lakes and to identify the measures to be taken to reduce water quality problems associated with the ... [more ▼]There is a strong interest in developing a capacity to predict the occurrence of cyanobacteria blooms in lakes and to identify the measures to be taken to reduce water quality problems associated with the occurrence of potentially harmful taxa. Here we conducted a weekly to bi-weekly monitoring program on five shallow eutrophic lakes during two years, with the aim of gathering data on total cyanobacterial abundance, as estimated from marker pigments determined by HPLC analysis of phytoplankton extracts. We also determined bloom composition and measured weather and limnological variables. The most frequently identified taxa were Aphanizomenon flos-aquae, Microcystis aeruginosa, Planktothrix agardhii and Anabaena spp. We used the data base composed of a total of 306 observations and an adaptive regression trees method, the boosted regression tree (BRT), to develop predictive models of bloom occurrence and composition, based on environmental conditions. Data processing with BRT enabled the design of satisfactory prediction models of cyanobacterial abundance and of the occurrence of the main taxa. Phosphorus (total and soluble reactive phosphate), dissolved inorganic nitrogen, epilimnion temperature, photoperiod and euphotic depth were among the best predictive variables, contributing for at least 10 % in the models, and their relative contribution varied in accordance with the ecological traits of the taxa considered. Meteorological factors (wind, rainfall, surface irradiance) had a significant role in species selection. Such results may contribute to designing measures for bloom management in shallow lakes. [less ▲]Detailed reference viewed: 15 (1 ULg) Manipulation et mobilisation en cas de cervicalgieDemoulin, Christophe in Cochrane (2016)Detailed reference viewed: 21 (2 ULg) Blowing a liquid curtainLhuissier, Henri; Brunet, Philippe; Dorbolo, Stéphane in Journal of Fluid Mechanics (2016), 795Detailed reference viewed: 23 (5 ULg) Association Between Necropsy Evidence of Disseminated Intravascular Coagulation and Hemostatic Variables Before Death in Horses With Colic.Cesarini Latorre, Carlota ; Cotovio, M.; Rios, J. et alin Journal of veterinary internal medicine / American College of Veterinary Internal Medicine (2016), 30(1), 269-75BACKGROUND: Disseminated intravascular coagulation (DIC) is frequent in horses with severe gastrointestinal disorders. Postmortem studies have found fibrin microthrombi in tissues of these horses, but ... [more ▼]BACKGROUND: Disseminated intravascular coagulation (DIC) is frequent in horses with severe gastrointestinal disorders. Postmortem studies have found fibrin microthrombi in tissues of these horses, but studies relating these histopathological findings with antemortem hemostatic data are lacking. HYPOTHESIS: Antemortem classification of coagulopathy is related to the presence and severity of fibrin deposits observed postmortem in horses with severe gastrointestinal disorders. ANIMALS: Antemortem hemostatic profile data and postmortem tissue samples (kidney, lung, liver) from 48 horses with colic. METHODS: Tissue samples were stained with phosphotungstic acid hematoxylin and immunohistochemical methods for histological examination. A fibrin score (grades 0-4) was assigned for each technique, tissue and horse, as well as the presence or absence of DIC at postmortem examination. D-dimer concentration, prothrombin time (PT), activated partial thromboplastin time (aPTT), and antithrombin (AT) activity, as well as the clinicopathological evidence of coagulopathy, were determined from plasma samples collected 0-24 hours before death or euthanasia. Histologic and clinicopathologic data from the same horses were compared retrospectively. RESULTS: No association was found between antemortem classification of coagulopathy and postmortem diagnosis of DIC based on tissue fibrin deposition. None of the hemostatic parameters was significantly different between horses with or without postmortem diagnosis of DIC. There was no association between horses with fibrin in tissues or different cut-offs for D-dimer concentration and postmortem evidence of DIC. CONCLUSIONS AND CLINICAL IMPORTANCE: Abnormalities of the routine clotting profile, including D-dimer concentration, were not useful in predicting histologic evidence of DIC at necropsy in horses with severe gastrointestinal disorders. [less ▲]Detailed reference viewed: 9 (1 ULg) Surgical and interventional management of mitral valve regurgitation: a position statement from the European Society of Cardiology Working Groups on Cardiovascular Surgery and Valvular Heart Disease.De Bonis, Michele; Al-Attar, Nawwar; Antunes, Manuel et alin European heart journal (2016)Detailed reference viewed: 23 (3 ULg) Like a bridge over troubled water – opening pathways for integrating social sciences and humanities into nuclear researchTurcanu, Catrinel; Schröder, Jantine; Meskens, Gaston et alin Journal of Environmental Radioactivity (2016), 153Research on nuclear technologies has been largely driven by a detachment of the 'technical content' from the 'social context'. However, social studies of science and technology - also for the nuclear ... [more ▼]Research on nuclear technologies has been largely driven by a detachment of the 'technical content' from the 'social context'. However, social studies of science and technology - also for the nuclear domain – emphasize that 'the social' and 'the technical' dimensions of technology development are inter-related and co-produced. In an effort to create links between nuclear research and innovation and society in mutually beneficial ways, the Belgian Nuclear Research Centre started fifteen years ago a ‘Programme of Integration of Social Aspects into nuclear research’ (PISA). In line with broader science-policy agendas (responsible research and innovation and technology assessment), this paper argues that the importance of such programmes is threefold. First, their multi-disciplinary basis and participatory character contribute to a better understanding of the interactions between science, technology and society, in general, and the complexity of nuclear technology assessment in particular. Second, their functioning as (self )critical policy supportive research with outreach to society is an essential prerequisite for policies aiming at generating societal trust in the context of controversial issues related to nuclear technologies and exposure to ionising radiation. Third, such programmes create an enriching dynamic in the organisation itself, stimulating collective learning and transdisciplinarity. The paper illustrates with concrete examples these claims and concludes by discussing some key challenges that researchers face while engaging in work of this kind. [less ▲]Detailed reference viewed: 31 (0 ULg) Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells.Mendes, Luis Filipe; Tam, Wai Long; Chai, Yoke Chin et alin Tissue Engineering. Part C, Methods (2016), 22(5), 473-86Successful application of cell-based strategies in cartilage and bone tissue engineering has been hampered by the lack of robust protocols to efficiently differentiate mesenchymal stem cells into the ... [more ▼]Successful application of cell-based strategies in cartilage and bone tissue engineering has been hampered by the lack of robust protocols to efficiently differentiate mesenchymal stem cells into the chondrogenic lineage. The development of chemically defined culture media supplemented with growth factors (GFs) has been proposed as a way to overcome this limitation. In this work, we applied a fractional design of experiment (DoE) strategy to screen the effect of multiple GFs (BMP2, BMP6, GDF5, TGF-beta1, and FGF2) on chondrogenic differentiation of human periosteum-derived mesenchymal stem cells (hPDCs) in vitro. In a micromass culture (muMass) system, BMP2 had a positive effect on glycosaminoglycan deposition at day 7 (p < 0.001), which in combination with BMP6 synergistically enhanced cartilage-like tissue formation that displayed in vitro mineralization capacity at day 14 (p < 0.001). Gene expression of muMasses cultured for 7 days with a medium formulation supplemented with 100 ng/mL of BMP2 and BMP6 and a low concentration of GDF5, TGF-beta1, and FGF2 showed increased expression of Sox9 (1.7-fold) and the matrix molecules aggrecan (7-fold increase) and COL2A1 (40-fold increase) compared to nonstimulated control muMasses. The DoE analysis indicated that in GF combinations, BMP2 was the strongest effector for chondrogenic differentiation of hPDCs. When transplanted ectopically in nude mice, the in vitro-differentiated muMasses showed maintenance of the cartilaginous phenotype after 4 weeks in vivo. This study indicates the power of using the DoE approach for the creation of new medium formulations for skeletal tissue engineering approaches. [less ▲]Detailed reference viewed: 6 (0 ULg) Reference Method and Reference Material Are Necessary Tools to Reveal the Variability of Cystatin C AssaysBargnoux, Anne-Sophie; Kuster, Nils; Delatour, Vincent et alin Archives of Pathology & Laboratory Medicine (2016), 140(2), 117-118Detailed reference viewed: 43 (4 ULg) Review of dietary supplements for the management of osteoarthritis in dogs in studies from 2004 to 2014Comblain, Fanny ; Serisier, Samuel; Barthelemy, Nicolas et alin Journal of Veterinary Pharmacology & Therapeutics (2016), 39Detailed reference viewed: 141 (8 ULg) Reduction in cardiovascular and all-cause mortality in the EMPA-REG OUTCOME trial: A critical analysis.SCHEEN, André in Diabetes & metabolism (2016), 42(2), 71-6Detailed reference viewed: 27 (4 ULg) Combining feature extraction methods to assist the diagnosis of Alzheimer's diseaseSegovia, Fermin; Górriz, J. M.; Ramírez, J. et alin Current Alzheimer Research (2016), 13Neuroimaging data as 18F-FDG PET is widely used to assist the diagnosis of Alzheimer’s disease (AD). Looking for regions with hypoperfusion/ hypometabolism, clinicians may predict or corroborate the ... [more ▼]Neuroimaging data as 18F-FDG PET is widely used to assist the diagnosis of Alzheimer’s disease (AD). Looking for regions with hypoperfusion/ hypometabolism, clinicians may predict or corroborate the diagnosis of the patients. Modern computer aided diagnosis (CAD) systems based on the statistical analysis of whole neuroimages are more accurate than classical systems based on quantifying the uptake of some predefined regions of interests (ROIs). In addition, these new systems allow determining new ROIs and take advantage of the huge amount of information comprised in neuroimaging data. A major branch of modern CAD systems for AD is based on multivariate techniques, which analyse a neuroimage as a whole, considering not only the voxel intensities but also the relations among them. In order to deal with the vast dimensionality of the data, a number of feature extraction methods have been successfully applied. In this work, we propose a CAD system based on the combination of several feature extraction techniques. First, some commonly used feature extraction methods based on the analysis of the variance (as principal component analysis), on the factorization of the data (as non-negative matrix factorization) and on classical magnitudes (as Haralick features) were simultaneously applied to the original data. These feature sets were then combined by means of two different combination approaches: i) using a single classifier and a multiple kernel learning approach and ii) using an ensemble of classifier and selecting the final decision by majority voting. The proposed approach was evaluated using a labelled neuroimaging database along with a cross validation scheme. As conclusion, the proposed CAD system performed better than approaches using only one feature extraction technique. We also provide a fair comparison (using the same database). [less ▲]Detailed reference viewed: 104 (21 ULg) Coupling curvature-dependent and shear stress-stimulated neotissue growth in dynamic bioreactor cultures: a 3D computational model of a complete scaffold.Guyot, Y.; Papantoniou, I.; Luyten, F. P. et alin Biomechanics & Modeling in Mechanobiology (2016), 15(1), 169-80The main challenge in tissue engineering consists in understanding and controlling the growth process of in vitro cultured neotissues toward obtaining functional tissues. Computational models can provide ... [more ▼]The main challenge in tissue engineering consists in understanding and controlling the growth process of in vitro cultured neotissues toward obtaining functional tissues. Computational models can provide crucial information on appropriate bioreactor and scaffold design but also on the bioprocess environment and culture conditions. In this study, the development of a 3D model using the level set method to capture the growth of a microporous neotissue domain in a dynamic culture environment (perfusion bioreactor) was pursued. In our model, neotissue growth velocity was influenced by scaffold geometry as well as by flow- induced shear stresses. The neotissue was modeled as a homogenous porous medium with a given permeability, and the Brinkman equation was used to calculate the flow profile in both neotissue and void space. Neotissue growth was modeled until the scaffold void volume was filled, thus capturing already established experimental observations, in particular the differences between scaffold filling under different flow regimes. This tool is envisaged as a scaffold shape and bioprocess optimization tool with predictive capacities. It will allow controlling fluid flow during long-term culture, whereby neotissue growth alters flow patterns, in order to provide shear stress profiles and magnitudes across the whole scaffold volume influencing, in turn, the neotissue growth. [less ▲]Detailed reference viewed: 5 (0 ULg) ntegrating sediment biogeochemistry into 3D oceanic models: A study of benthic-pelagic coupling in the Black SeaCapet, Arthur ; Meysman, Filip; Akoumianaki, Ioanna et alin Ocean Modelling (2016), 101Three-dimensional (3D) ecosystem models of shelf environments should properly account for the biogeochemical cycling within the sea floor. However, a full and explicit representation of sediment ... [more ▼]Three-dimensional (3D) ecosystem models of shelf environments should properly account for the biogeochemical cycling within the sea floor. However, a full and explicit representation of sediment biogeochemistry into 3D ocean models is computationally demanding. Here, we describe a simplified approach to include benthic processes in 3D ocean models, which includes a parameterization of the different pathways for organic matter mineralization and allows for organic matter remobilization by bottom currents and waves. This efficient approach enables decadal simulations that resolve the inertial contribution of the sea floor to the biogeochemical cycling in shelf environments. The model was implemented to analyze the benthic-pelagic coupling in the northwestern shelf of the Black Sea. Three distinct biogeochemical provinces were identified on the basis of fluxes and rates associated with benthic-pelagic coupling. Our model simulations suitably capture the seasonal variability of in situ flux data as well as their regional variation, which stresses the importance of incorporating temporally varying sediment biogeochemistry and resuspension/redeposition cycles in shelf ecosystem models. [less ▲]Detailed reference viewed: 7 (0 ULg) A Mixed Integer Programming formulation for the three dimensional bin packing problem deriving from an air cargo applicationPaquay, Célia ; Schyns, Michael ; Limbourg, Sabine in International Transactions in Operational Research (2016), 23(1-2), 187-213The present paper looks into the problem of optimising the loading of boxes into containers. The goal is to minimise the unused volume. This type of problem belongs to the family of Multiple Bin Size Bin ... [more ▼]The present paper looks into the problem of optimising the loading of boxes into containers. The goal is to minimise the unused volume. This type of problem belongs to the family of Multiple Bin Size Bin Packing Problems. The approach includes an extensive set of constraints encountered in real-world applications in the three-dimensional case: the stability, the fragility of the items, the weight distribution and the possibility to rotate the boxes. It also includes the specific situation in which containers are truncated parallelepipeds. This is typical in the field of air transportation. While most papers on cutting and packing problems describe ad-hoc procedures, this paper proposes a mixed integer linear program. The validity of this model is tested on small instances. [less ▲]Detailed reference viewed: 192 (55 ULg) Logistics Service Quality: The Case of Da Nang CityLimbourg, Sabine ; Giang, Ho Thi Quynh; Cools, Mario in Procedia Engineering (2016), 142Cost minimization is a key objective in managing logistics services. In this context, a growing interest in quality improvement can be observed. However, service quality is hard to quantify as it is a ... [more ▼]Cost minimization is a key objective in managing logistics services. In this context, a growing interest in quality improvement can be observed. However, service quality is hard to quantify as it is a function of varying customer perceptions over time, of the measurement process and of the analysis of the data gathered. This study examines the logistics service quality by scales developed according to SERVQUAL instrument. A survey of 200 customers of logistics service providers (LSP) was conducted in Da Nang City. The data analysis shows that LSPs in Da Nang city need to improve Research and Development and to develop Customer Care programs. [less ▲]Detailed reference viewed: 27 (6 ULg) Predictions of PinningParuch, Patrycja; Ghosez, Philippe in Nature (2016), 534A multiscale model has been implemented that provides accurate predictions of the behaviour of ferroelectric materials in electric fields, and might aid efforts to design devices such as sensors and ... [more ▼]A multiscale model has been implemented that provides accurate predictions of the behaviour of ferroelectric materials in electric fields, and might aid efforts to design devices such as sensors and digital memory. [less ▲]Detailed reference viewed: 17 (3 ULg) Differential Expression of Genes and DNA Methylation associated with Prenatal Protein Undernutrition by Albumen Removal in an avian modelWillems, E.; Guerrero-Bosagna, C.; Decuypere, E. et alin Scientific Reports (2016), 6Previously, long-term effects on body weight and reproductive performance have been demonstrated in the chicken model of prenatal protein undernutrition by albumen removal. Introduction of such persistent ... [more ▼]Previously, long-term effects on body weight and reproductive performance have been demonstrated in the chicken model of prenatal protein undernutrition by albumen removal. Introduction of such persistent alterations in phenotype suggests stable changes in gene expression. Therefore, a genomewide screening of the hepatic transcriptome by RNA-Seq was performed in adult hens. The albumendeprived hens were created by partial removal of the albumen from eggs and replacement with saline early during embryonic development. Results were compared to sham-manipulated hens and non-manipulated hens. Grouping of the differentially expressed (DE) genes according to biological functions revealed the involvement of processes such as 'embryonic and organismal development' and 'reproductive system development and function'. Molecular pathways that were altered were 'amino acid metabolism', 'carbohydrate metabolism' and 'protein synthesis'. Three key central genes interacting with many DE genes were identified: UBC, NR3C1, and ELAVL1. The DNA methylation of 9 DE genes and 3 key central genes was examined by MeDIP-qPCR. The DNA methylation of a fragment (UBC-3) of the UBC gene was increased in the albumen-deprived hens compared to the nonmanipulated hens. In conclusion, these results demonstrated that prenatal protein undernutrition by albumen removal leads to long-term alterations of the hepatic transcriptome in the chicken. [less ▲]Detailed reference viewed: 6 (1 ULg) SRC Kinase Inhibition with Saracatinib Limits the Development of Osteolytic Bone Disease in Multiple MyelomaHeusschen, Roy ; Muller, Joséphine ; Binsfeld, Marilène et alin Oncotarget (2016)Detailed reference viewed: 21 (3 ULg) Farmers’ Perception and Willingness to Pay for Climate Information in Burkina FasoZongo, Bétéo ; Diarra, Abdoulaye; Barbier, Bruno et alin Journal of Agricultural Science (2016), 8This study highlights the perception and the option value of climate information in the sahelian and sudano-sahelian agro-climatic zones of Burkina Faso. From the sampled farmers only 21.78% have access ... [more ▼]This study highlights the perception and the option value of climate information in the sahelian and sudano-sahelian agro-climatic zones of Burkina Faso. From the sampled farmers only 21.78% have access to climate information, while 93% of them need climate information to guide through their decision in planning agricultural activities. Option value shows the interest granted by farmers using climate information. About 64% of the farmers are willing to pay an average of Francs CFA 546.34 to obtain climate information. The willingness to pay is determined by the the farmers’ ability to predict the climate, to use radio as a means of information, farmers’ awareness of previous forecasts and early onset of rainy season. While farmers considered benefits from the use of climate information, it is clear that its contribution to farm income remains a field of research to explore. Thus it is necessary to experiment with individual farms and to evaluate the contribution of climate information to the value added in different crops and farmers’ income. [less ▲]Detailed reference viewed: 28 (3 ULg) review of Frédéric Duval, Les mots de l'édition de textesMorato, Nicola in French Studies (2016), 70(2), Detailed reference viewed: 22 (1 ULg) The unexpected structure of the designed protein Octarellin V.1 forms a challenge for protein structure prediction tools.Figueroa Yévenes, Maximiliano ; Sleutel, Mike; Vandevenne, Marylène et alin Journal of structural biology (2016)Despite impressive successes in protein design, designing a well-folded protein of more 100 amino acids de novo remains a formidable challenge. Exploiting the promising biophysical features of the ... [more ▼]Despite impressive successes in protein design, designing a well-folded protein of more 100 amino acids de novo remains a formidable challenge. Exploiting the promising biophysical features of the artificial protein Octarellin V, we improved this protein by directed evolution, thus creating a more stable and soluble protein: Octarellin V.1. Next, we obtained crystals of Octarellin V.1 in complex with crystallization chaperons and determined the tertiary structure. The experimental structure of Octarellin V.1 differs from its in silico design: the (alphabetaalpha) sandwich architecture bears some resemblance to a Rossman-like fold instead of the intended TIM-barrel fold. This surprising result gave us a unique and attractive opportunity to test the state of the art in protein structure prediction, using this artificial protein free of any natural selection. We tested 13 automated webservers for protein structure prediction and found none of them to predict the actual structure. More than 50% of them predicted a TIM-barrel fold, i.e. the structure we set out to design more than 10years ago. In addition, local software runs that are human operated can sample a structure similar to the experimental one but fail in selecting it, suggesting that the scoring and ranking functions should be improved. We propose that artificial proteins could be used as tools to test the accuracy of protein structure prediction algorithms, because their lack of evolutionary pressure and unique sequences features. [less ▲]Detailed reference viewed: 17 (3 ULg)