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See detailAssessing inter- and intramolecular continuous-flow strategies towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULg; Winter, Marc; Vizza, Alessandra et al

in Reaction Chemistry and Engineering (2017)

The batch-to-flow translation of inter- and intramolecular strategies for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. Both ... [more ▼]

The batch-to-flow translation of inter- and intramolecular strategies for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. Both inter- and intramolecular strategies imply the telescoping of multiple processing steps and the generation of unstable diazo species under continuous-flow conditions. The intermolecular strategy relies on an unprecedented continuous-flow Rh-catalyzed intermolecular C–H carbene insertion, providing enriched threo-N-Boc methylphenidate in 38% or 19% isolated yield according to sequential or fully telescoped processes, respectively. Quantitative Boc-deprotection is carried out off-line. The intramolecular strategy relies on a continuous-flow thermal intramolecular C–H carbene insertion, providing enriched threo-methylphenidate hydrochloride in 70% isolated yield. A continuous-flow photochemical alternative is also presented. The critical step of the most promising intramolecular strategy is implemented on the mesoscale in a pilot-scale continuous-flow reactor. [less ▲]

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See detailQuantification of Calabrian vegetation in Southern Primory'e (far East of Russia) using multiple proxies
Bondarenko, O. V.; Blokhina, N. I.; Bruch, A. A. et al

in Palaeogeography, Palaeoclimatology, Palaeoecology (2017), 467

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See detailL'intervention de l'assureur dans le procès pénal : la défense de ton assuré tu assumeras ?
Michiels, Olivier ULg

in Revue de la Faculté de Droit de l'Université de Liège (2017), 2017/1

L'article aborde l'intervention de la compagnie d'assurance dans le procès pénal. Il envisage le contenu de l'article 153 § 5 de la loi du 4 avril 2014 relative aux assurances, les possibilités de défense ... [more ▼]

L'article aborde l'intervention de la compagnie d'assurance dans le procès pénal. Il envisage le contenu de l'article 153 § 5 de la loi du 4 avril 2014 relative aux assurances, les possibilités de défense de l'assureur du prévenu face à la personne lésée et les incidences des moyens et prétentions de l'assureur sur la défense pénale de son assuré prévenu. [less ▲]

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See detailInteractive effects of ultraviolet radiation and thermal stress on two reef-building corals
Courtial, Lucile; Roberty, Stéphane ULg; Shick, Malcom et al

in Limnology and Oceanography (2017)

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See detailVulnerability evaluation of millet and sorghum cropping system to climate change and adoption of new technologies in Mali
Sissoko, Penda ULg; Aune, Jens B.; Senneväg, Gry et al

in Asian Journal of Science and Technology (2017), 8(1), 4176-4182

This study analyzes the vulnerability of agricultural cropping systems based on millet and sorghum and the adoption of new technologies in Sahelian and Sudano - Sahelian areas in Mali (West Africa). Semi ... [more ▼]

This study analyzes the vulnerability of agricultural cropping systems based on millet and sorghum and the adoption of new technologies in Sahelian and Sudano - Sahelian areas in Mali (West Africa). Semi-structured interviews (ISS) using questionnaires addressed to the chiefs of family farm (FAE) were used for the collection of data. The descriptive statistics and matrices were realized for the data analysis using the following softwares: EXCEL and SPSS20.The results of the surveys conducted in 2014 among 360 farms in Sahelian and Sudano-Sahelian areas, shows that agricultural producers are aware of the main factors affecting the productivity of their cropping system and food security of agricultural holdings. Rainfall variability, characterized by failure and irregularity of rains is the main constraint of cropping systems based on millet and sorghum as stated by 97% of surveyed producers, followed by soil poverty 81%, and the lack of agricultural equipment 47%. The impacts of these factors at the farms level are translated by a decrease in the crops yields and on the frequency of the shortages in foodstuffs which are observed by 98% of the farms every year and more than 50% every 3 years. To reduce vulnerabilities, practices and innovations were adopted by farms as strategies. The main concern fertilizer micro dosing technics, seed soaking, improved varieties, organic and mineral fertilization, soils and water management technics, mechanical sowing, and agro forestry were adopted as strategies. [less ▲]

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See detailMemory consolidation in children with Specific Language Impairment: Delayed gains and susceptibility to interference in implicit sequence learning
Desmottes, Lise ULg; Maillart, Christelle ULg; Meulemans, Thierry ULg

in Journal of Clinical and Experimental Neuropsychology (2017)

Introduction: In this study, the time course of the procedural learning of a visuomotor sequence skill was followed over a 24-hour and a 1-week time period in children with and without specific language ... [more ▼]

Introduction: In this study, the time course of the procedural learning of a visuomotor sequence skill was followed over a 24-hour and a 1-week time period in children with and without specific language impairment (SLI). Two aspects of memory consolidation in implicit sequence learning were examined: the evolution of post-training gains in sequence knowledge (Experiment 1) and the susceptibility to interference (Experiment 2). Method and Results: In the first experiment, 18 children with SLI and 17 control children matched for sex, age, and nonverbal intelligence completed a serial reaction-time (SRT) task and were tested 24 hours and 1 week after practicing. The two groups of children attained an equal level of sequence knowledge in the training session, but the children with SLI lacked the consolidation gains displayed by the control children in the two post-training sessions. Working with a new group of children, 17 with SLI and 17 control peers, Experiment 2 examined resistance to interference by introducing a second sequence 15 minutes after the first training session. Similar results were obtained for the performance of both groups in the training session. However, although the performance of the control group improved in the post-training sessions, the performance of the SLI group deteriorated significantly during the consolidation phase due to the interfering sequence. Conclusion: These findings suggest that the consolidation phase of sequence learning is impaired in children with SLI. [less ▲]

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See detailExome copy number variation detection: Use of a pool of unrelated healthy tissue as reference sample
Wenric, Stéphane ULg; Sticca, Tiberio ULg; CABERG, Jean-Hubert ULg et al

in Genetic Epidemiology (2017)

An increasing number of bioinformatic tools designed to detect CNVs (copy number variants) in tumor samples based on paired exome data where a matched healthy tissue constitutes the reference have been ... [more ▼]

An increasing number of bioinformatic tools designed to detect CNVs (copy number variants) in tumor samples based on paired exome data where a matched healthy tissue constitutes the reference have been published in the recent years. The idea of using a pool of unrelated healthy DNA as reference has previously been formulated but not thoroughly validated. As of today, the gold standard for CNV calling is still aCGH but there is an increasing interest in detecting CNVs by exome sequencing. We propose to design a metric allowing the comparison of two CNV profiles, independently of the technique used and assessed the validity of using a pool of unrelated healthy DNA instead of a matched healthy tissue as reference in exome-based CNV detection. We compared the CNV profiles obtained with three different approaches (aCGH, exome sequencing with a matched healthy tissue as reference, exome sequencing with a pool of eight unrelated healthy tissue as reference) on three multiple myeloma samples. We show that the usual analyses performed to compare CNV profiles (deletion/amplification ratios and CNV size distribution) lack in precision when confronted with low LRR values, as they only consider the binary status of each CNV. We show that the metric-based distance constitutes a more accurate comparison of two CNV profiles. Based on these analyses, we conclude that a reliable picture of CNV alterations in multiple myeloma samples can be obtained from whole-exome sequencing in the absence of a matched healthy sample. [less ▲]

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See detailSustainability and management control. Exploring and theorizing control patterns in large European firms
Crutzen, Nathalie ULg; Zvezdov, Dimitar; Schaltegger, Stefan

in Journal of Cleaner Production (2017), 143

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See detailPhytotoxic activity and chemical composition of Cassia absus seeds and aerial parts
Zribi, Ines; Sbai, H.; Ghezal, N. et al

in Natural Product Research (2017)

The present study was conducted to assess the phytotoxic potential and the phytochemical composition of Cassia absus. Aqueous extracts caused significant reduction of root growth of Lactuca sativa. Seed ... [more ▼]

The present study was conducted to assess the phytotoxic potential and the phytochemical composition of Cassia absus. Aqueous extracts caused significant reduction of root growth of Lactuca sativa. Seed extract was more effective than aerial part extract. Successive extractions of this plant were performed using solvents with increasing polarities. The methanolic seed extract exerted strong phytotoxic effect on seedling growth, followed by petroleum ether extract of the aerial part. The phytochemical investigation showed that among the organic extracts, methanol extracts of seeds and aerial parts contained the highest amounts of total phenolics and proanthocyanidins. Seeds were rich in linoleic acid followed by palmitic acids. Palmitic, stearic and arachidic acids were the major fatty acids in aerial parts. HPLC-DAD analysis of the methanolic extracts revealed the presence of luteolin in C. absus aerial parts. [less ▲]

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See detailReproducibility of tract-based white matter microstructural measures using the ENIGMA-DTI protocol.
Acheson, Ashley; Wijtenburg, S. Andrea; Rowland, Laura M. et al

in Brain and Behavior (2017), 7(2), 00615

BACKGROUND: In preparation for longitudinal analyses of white matter development in youths with family histories of substance use disorders (FH+) or without such histories (FH-), we examined the ... [more ▼]

BACKGROUND: In preparation for longitudinal analyses of white matter development in youths with family histories of substance use disorders (FH+) or without such histories (FH-), we examined the reproducibility and reliability of global and regional measures of fractional anisotropy (FA) values, measured using the Enhancing Neuro Imaging Genetics Through Meta Analysis (ENIGMA)-diffusion tensor imaging (DTI) protocol. Highly reliable measures are necessary to detect any subtle differences in brain development. METHODS: First, we analyzed reproducibility data in a sample of 12 healthy young adults (ages 20-28) imaged three times within a week. Next, we calculated the same metrics in data collected 1-year apart in the sample of 68 FH+ and 21 FH- adolescents. This is a timeframe where within subject changes in white matter microstructure are small compared to between subject variance. Reproducibility was estimated by examining mean coefficients of variation (MCV), mean absolute differences (MAD), and intraclass correlations (ICC) for global and tract-specific FA values. RESULTS: We found excellent reproducibility for whole-brain DTI-FA values and most of the white matter tracts, except for the corticospinal tract and the fornix in both adults and youths. There was no significant effect of FH-group on reproducibility (p = .4). Reproducibility metrics were not significantly different between adolescents and adults (all p > .2). In post hoc analyses, the reproducibility metrics for regional FA values showed a strong positive correlation (r = .6) with the regional FA heritability measures previously reported by ENIGMA-DTI. CONCLUSION: Overall, this study demonstrated an excellent reproducibility of ENIGMA-DTI FA, positing it as viable analysis tools for longitudinal studies and other protocols that repeatedly assess white matter microstructure. [less ▲]

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See detailFast In-Plane Dynamics of a Beam with Unilateral Constraints
Liakou, Anna; Denoël, Vincent ULg; Detournay, Emmanuel

in Journal of Engineering Mechanics (2017), 0(0), 04016116

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See detailA robust statistical approach to select adequate error distributions for financial returns
Hambuckers, julien ULg; Heuchenne, Cédric ULg

in Journal of Applied Statistics (2017), 44(1), 137-161

In this article, we propose a robust statistical approach to select an appropriate error distribution, in a classical multiplicative heteroscedastic model. In a first step, unlike to the traditional ... [more ▼]

In this article, we propose a robust statistical approach to select an appropriate error distribution, in a classical multiplicative heteroscedastic model. In a first step, unlike to the traditional approach, we don't use any GARCH-type estimation of the conditional variance. Instead, we propose to use a recently developed nonparametric procedure (Mercurio and Spokoiny, 2004): the Local Adaptive Volatility Estimation (LAVE). The motivation for using this method is to avoid a possible model misspecification for the conditional variance. In a second step, we suggest a set of estimation and model selection procedures (Berk-Jones tests, kernel density-based selection, censored likelihood score, coverage probability) based on the so-obtained residuals. These methods enable to assess the global fit of a set of distributions as well as to focus on their behavior in the tails, giving us the capacity to map the strengths and weaknesses of the candidate distributions. A bootstrap procedure is provided to compute the rejection regions in this semiparametric context. Finally, we illustrate our methodology throughout a small simulation study and an application on three time series of daily returns (UBS stock returns, BOVESPA returns and EUR/USD exchange rates) [less ▲]

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See detailSingle- or double-unit UCBT following RIC in adults with AL: a report from Eurocord, the ALWP and the CTIWP of the EBMT.
Baron, Frédéric ULg; Ruggeri, Annalisa; Beohou, Eric et al

in Journal of Hematology & Oncology (2017), 10(1), 128

BACKGROUND: The feasibility of cord blood transplantation (CBT) in adults is limited by the relatively low number of hematopoietic stem/progenitor cells contained in one single CB unit. The infusion of ... [more ▼]

BACKGROUND: The feasibility of cord blood transplantation (CBT) in adults is limited by the relatively low number of hematopoietic stem/progenitor cells contained in one single CB unit. The infusion of two CB units from different partially HLA-matched donors (double CBT) is frequently performed in patients who lack a sufficiently rich single CB unit. METHODS: We compared CBT outcomes in patients given single or double CBT following reduced-intensity conditioning (RIC) in a retrospective multicenter registry-based study. Inclusion criteria included adult (>/=18 years) patients, acute myeloid leukemia (AML) or acute lymphoblastic leukemia (ALL), complete remission (CR) at the time of transplantation, first single (with a cryopreserved TNC >/= 2.5 x 107/kg) or double CBT between 2004 and 2014, and RIC conditioning. RESULTS: Data from 534 patients with AML (n = 408) or ALL (n = 126) receiving a first single (n = 172) or double (n = 362) CBT were included in the analyses. In univariate analysis, in comparison to patients transplanted with a single CB, double CB recipients had a similar incidence of neutrophil engraftment but a suggestion for a higher incidence of grade II-IV acute GVHD (36 versus 28%, P = 0.08). In multivariate analyses, in comparison to single CBT recipients, double CBT patients had a comparable incidence of relapse (HR = 0.9, P = 0.5) and of nonrelapse mortality (HR = 0.8, P = 0.3), as well as comparable overall (HR = 0.8, P = 0.17), leukemia-free (HR = 0.8, P = 0.2) and GVHD-free, relapse-free (HR = 1.0, P = 0.3) survival. CONCLUSIONS: These data failed to demonstrate better transplantation outcomes in adult patients receiving double CBT in comparison to those receiving single CBT with adequate TNC after RIC. [less ▲]

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See detailAssessment of corrosion resistance, material properties, and weldability of alloyed steel for ballast tanks
De Baere, Kris; Verstraelen, Helen; Willemen, Remke et al

in Journal of Marine Science & Technology (2017), 22(1), 176-199

Ballast tanks are of great importance in the lifetime of modern merchant ships. Making a ballast tank less susceptible to corrosion can, therefore, prolong the useful life of a ship and, thereby, lower ... [more ▼]

Ballast tanks are of great importance in the lifetime of modern merchant ships. Making a ballast tank less susceptible to corrosion can, therefore, prolong the useful life of a ship and, thereby, lower its operational cost. An option to reinforce a ballast tank is to construct it out of a corrosion-resistant steel type. Such steel was recently produced by POSCO Ltd., South Korea. After 6 months of permanent immersion, the average corrosion rate of A and AH steel (31 samples) was 535 g m−2 year−1, while the Korean CRS was corroding with 378 g m−2 year−1. This entails a gain of 29 %. Follow-up measurements after 10, 20, and 24 months confirmed this. The results after 6 months exposure to alternating wet/dry conditions are even more explicit. Furthermore, the physical and metallurgical properties of this steel show a density of 7.646 t/m3, the elasticity modulus 209.3 GPa, the tensile strength 572 MPa, and the hardness 169HV10. Microscopically, the metal consists of equiaxed and recrystallized grains (ferrite and pearlite), with an average size of between 20 and 30 µm (ASTM E 112—12 grain size number between 7 and 8) with a few elongated pearlitic grains. The structure is banded ferrite/pearlite. On the basis of a series of energy dispersive X-ray spectrometer measurements the lower corrosion rate of the steel can be attributed to the interplay of Al, Cr, their oxides, and the corroding steel. In addition, the role of each element in the formation of oxide layers and the mechanisms contributing to the corrosion resistance are discussed. [less ▲]

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See detailPhase-change materials and rigidity
Piarristeguy, A.; Pradel, A.; Raty, Jean-Yves ULg

in MRS Bulletin (2017), 42(1), 45-49

Rigidity theory is an extraordinary tool to understand glasses. This article demonstrates how this model can help in understanding the link between structure, dynamics, and subtler properties such as ... [more ▼]

Rigidity theory is an extraordinary tool to understand glasses. This article demonstrates how this model can help in understanding the link between structure, dynamics, and subtler properties such as drift and aging, in particular, in phase-change materials (PCMs). First, a map of flexible/rigid regions in the Ge-(Sb)-Te system is drawn on the basis of atomistic structures modeled either by ab initio or reverse Monte Carlo techniques. A clear link between the flexible/rigid nature of the glass and its aging behavior is shown through resistivity drift as a function of composition measurements in amorphous GexTe100-x. In the particular case of amorphous GeTe, application of rigidity theory indicates that the average number of mechanical constraints decreases during aging, making the glass less stressed-rigid. Finally, the stability of PCMs also depends on the topology of the materials. The increasing number of constraints in GeTe when doped with C or N results in increased stability of the PCM. © 2017 Materials Research Society. [less ▲]

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See detailSoil organic carbon fractionation for improving agricultural soil quality assessment – a case study in Southern Belgium (Wallonia)
Trigalet, Sylvain; Chartin, Caroline; Krüger, Inken ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 21(S1),

Description of the subject. The paper presents and discusses a method for fractionating bulk soil organic carbon (SOC) in meaningful SOC fractions to better assess SOC status and its related soil ... [more ▼]

Description of the subject. The paper presents and discusses a method for fractionating bulk soil organic carbon (SOC) in meaningful SOC fractions to better assess SOC status and its related soil ecosystem functions. Objectives. The objective is to perform an evaluation of ecosystem functions of soil organic matter at plot scale and compare it to the normal operative range of the local agro-ecological region. Method. By separating carbon associated with clay and fine silt particles (stable carbon with slow turnover rate, < 20 μm) and carbon non-associated with this fraction (labile and intermediate carbon with higher turnover rates, ≥ 20 μm), effects of management can be detected more efficiently at different scales. Conclusions. Soil organic carbon fractions, used as proxies for soil ecosystem functions, can be helpful because they represent SOC functional pools. This paper proposes to apply fractionation on samples taken at plot and regional scale. It is therefore possible to establish a normal operative range for a specific agro-region for comparison with the values in individual plots. This allows drawing a baseline for SOC fractions status in a specific agricultural unit. This approach provides valuable information to study and evaluate the impact of agricultural management in the context of enhancing soil quality and functions. [less ▲]

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See detailPond drying cues and their effects on growth and metamorphosis in a fast developing amphibian
Székely, Diana ULg; Denoël, Mathieu ULg; Székely, Paul et al

in Journal of Zoology (2017)

The hydroperiod of breeding habitats imposes a strong selection on amphibians and pond-breeding species usually exhibit a high degree of plasticity in the duration of larval period. However, the potential ... [more ▼]

The hydroperiod of breeding habitats imposes a strong selection on amphibians and pond-breeding species usually exhibit a high degree of plasticity in the duration of larval period. However, the potential for phenotypic plasticity in fast developing species was investigated only in a small number of anurans, and the specific response to environmental cues such as low water versus decreasing water level, as well as the effects of such cues on particular developmental stages, are even less understood. In this context, we investigated the plastic response to pond desiccation in a neotropical species (Ceratophrys stolzmanni) by raising tadpoles in three water level treatments: constant high, constant low and decreasing. The growth rates were the highest reported for amphibian tadpoles (up to 0.3g/day) and the time to metamorphosis was short in all treatments, with the fastest developing tadpole metamorphosing in only 16 days after egg deposition. Individuals from the constant high water level treatment had a higher growth rate than those in the other two treatments, whereas decreasing and constant low water levels had similar effects on development, speeding up metamorphosis. In turn, this involved a cost as these tadpoles had a lower body size and mass at metamorphosis than the ones raised in constant high water levels. The final stages of metamorphosis, when tadpoles are the most vulnerable, were shorter in tadpoles exposed to a decreasing water level, allowing them to leave water quickly. Our experiment demonstrates that phenotypic plasticity is maintained even in environments devoid of permanent aquatic habitats. Ceratophryd tadpoles are able to shorten their developmental time when they perceive a risk of desiccation and react similarly to cues coming from the two unfavorable water conditions showing their adaptation to ephemeral and unpredictable breeding habitats. [less ▲]

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See detailStagnation of Saturn's auroral emission at noon
Radioti, Aikaterini ULg; Grodent, Denis ULg; Gérard, Jean-Claude ULg et al

in Journal of Geophysical Research. Space Physics (2017)

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See detailThe lipid 5-phoshatase SHIP2 controls renal brush border ultrastructure and function by regulating ERM proteins activation
Sayyed, Sufyan Ali ULg; JOURET, François ULg; Vermeersch, Marjorie et al

in Kidney International (2017)

The microvillus brush border on the renal proximal tubule epithelium allows the controlled reabsorption of solutes that are filtered through the glomerulus and thus participates in general body ... [more ▼]

The microvillus brush border on the renal proximal tubule epithelium allows the controlled reabsorption of solutes that are filtered through the glomerulus and thus participates in general body homeostasis. Here, using the lipid 5-phosphatase Ship2 global knock-out mice, proximal tubule-specific Ship2 knock-out mice and a proximal tubule cell model where SHIP2 is inactivated, we show that SHIP2 is a negative regulator of microvilli formation, thereby controlling solute reabsorption by the proximal tubule. We found increased PtdIns(4,5)P2 substrate and decreased PtdIns4P product when SHIP2 was inactivated, associated with hyperactivated Ezrin/Radixin/Moesin proteins and increased Rho-GTP. Thus, inactivation of SHIP2 leads to increased microvilli formation and solute reabsorption by the renal proximal tubule. This may represent an innovative therapeutic target for renal Fanconi syndromes characterized by decreased reabsorption of solutes by this nephron segment. [less ▲]

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See detailA French version of the Balanced Time Perspective Scale: Factor structure and relation to cognitive reappraisal.
Barsics, Catherine ULg; Rebetez, Marie; Rochat, Lucien et al

in Canadian Journal of Behavioural Science = Revue Canadienne des Sciences du Comportement (2017), 49(1), 51-57

A frequent and equal tendency to think positively about one’s past and future has been conceptualised as a balanced time perspective (TP). Such a dispositional temporal orientation has been associated ... [more ▼]

A frequent and equal tendency to think positively about one’s past and future has been conceptualised as a balanced time perspective (TP). Such a dispositional temporal orientation has been associated with higher life satisfaction and happiness. The aim of the present study was to develop and to validate a French version of the Balanced Time Perspective Scale (BTPS; Webster, 2011), which has been specifically designed to assess the combined use of positive future and past mental representations as resources for the self. Data were collected from an online survey in a sample of 622 French-speaking individuals from the general population. Results indicated that the French version of the BTPS replicated the 2-factor structure of the original questionnaire, and showed excellent internal consistency. External validity was supported by specific rela- tionships with measures of TP and positive affect. In addition, a high propensity to project oneself positively both in the future and the past was associated with greater use of cognitive reappraisal. [less ▲]

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See detailA nonlinear state-space approach to hysteresis identification
Noël, Jean-Philippe ULg; Esfahani, Alireza; Kerschen, Gaëtan ULg et al

in Mechanical Systems & Signal Processing (2017), 84

Most studies tackling hysteresis identification in the technical literature follow white-box approaches, i.e. they rely on the assumption that measured data obey a specific hysteretic model. Such an ... [more ▼]

Most studies tackling hysteresis identification in the technical literature follow white-box approaches, i.e. they rely on the assumption that measured data obey a specific hysteretic model. Such an assumption may be a hard requirement to handle in real applications, since hysteresis is a highly individualistic nonlinear behaviour. The present paper adopts a black-box approach based on nonlinear state-space models to identify hysteresis dynamics. This approach is shown to provide a general framework to hysteresis identification, featuring flexibility and parsimony of representation. Nonlinear model terms are constructed as a multivariate polynomial in the state variables, and parameter estimation is performed by minimising weighted least-squares cost functions. Technical issues, including the selection of the model order and the polynomial degree, are discussed, and model validation is achieved in both broadband and sine conditions. The study is carried out numerically by exploiting synthetic data generated via the Bouc-Wen equations. [less ▲]

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See detailIconic virtues of diagrams. Peirce on ampliative reasoning
Leclercq, Bruno ULg

in Signata. Annales des Sémiotiques = Annals of Semiotics (2017)

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See detailEcotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids
Gismondi, Eric ULg; Thomé, Jean-Pierre ULg; Urien, Nastassia et al

in Environmental Pollution (2017)

Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health ... [more ▼]

Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures to Cd, Cu or Pb, at environmentally realistic concentrations (i.e. 0.25, 1.5 and 5 µg/L respectively). After 10-week exposure, changes in protein expression were investigated in caeca of control and exposed males. Gel-free proteomic analyses allowed for the identification of 35 proteins involved in various biological functions, for which 23 were significantly deregulated by metal exposures. The protein deregulation profiles were specific to each metal, providing evidence for metal-specific action sites and responses of gammarids. Among the tested metals, Cu was the most toxic in terms of mortality, probably linked with persistent oxidative stress. Moulting and osmoregulation were the major biological functions affected by Cu in the long-term. In Pb-exposed gammarids, significant deregulations of proteins involved in immune response and cytoskeleton were observed. Reproduction appears to be strongly affected in gammarids chronically exposed to Cd or Pb. Besides, modified expressions of several proteins involved in energy transfer and metabolism highlighted important energetic reshuffling to cope with chronic metal exposures. These results support the fact that metallic pressures induce a functional and energetic cost for individuals of G. fossarum with potential repercussions on population dynamics. Furthermore, this ecotoxicoproteomic study offers promising lines of enquiry in the development of new biomarkers that could make evidence of long-term impacts of metals on the health of organisms. [less ▲]

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See detailA Large and Consistent Phylogenomic Dataset Supports Sponges as the Sister Group to All Other Animals.
Simion, Paul; Philippe, Herve; Baurain, Denis ULg et al

in Current Biology (2017)

Resolving the early diversification of animal lineages has proven difficult, even using genome-scale datasets. Several phylogenomic studies have supported the classical scenario in which sponges (Porifera ... [more ▼]

Resolving the early diversification of animal lineages has proven difficult, even using genome-scale datasets. Several phylogenomic studies have supported the classical scenario in which sponges (Porifera) are the sister group to all other animals ("Porifera-sister" hypothesis), consistent with a single origin of the gut, nerve cells, and muscle cells in the stem lineage of eumetazoans (bilaterians + ctenophores + cnidarians). In contrast, several other studies have recovered an alternative topology in which ctenophores are the sister group to all other animals (including sponges). The "Ctenophora-sister" hypothesis implies that eumetazoan-specific traits, such as neurons and muscle cells, either evolved once along the metazoan stem lineage and were then lost in sponges and placozoans or evolved at least twice independently in Ctenophora and in Cnidaria + Bilateria. Here, we report on our reconstruction of deep metazoan relationships using a 1,719-gene dataset with dense taxonomic sampling of non-bilaterian animals that was assembled using a semi-automated procedure, designed to reduce known error sources. Our dataset outperforms previous metazoan gene superalignments in terms of data quality and quantity. Analyses with a best-fitting site-heterogeneous evolutionary model provide strong statistical support for placing sponges as the sister-group to all other metazoans, with ctenophores emerging as the second-earliest branching animal lineage. Only those methodological settings that exacerbated long-branch attraction artifacts yielded Ctenophora-sister. These results show that methodological issues must be carefully addressed to tackle difficult phylogenetic questions and pave the road to a better understanding of how fundamental features of animal body plans have emerged. [less ▲]

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See detailEfficient ANOVA for directional data
Ley, Christophe; Swan, Yvik ULg; Verdebout, Thomas

in Annals of the Institute of Statistical Mathematics (2017)

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See detailA test field for Gaia. Radial velocity catalogue of stars in the South Ecliptic Pole
Frémat, Y.; Altmann, M.; Pancino, E. et al

in Astronomy and Astrophysics (2017), 597

Context. Gaia is a space mission that is currently measuring the five astrometric parameters, as well as spectrophotometry of at least 1 billion stars to G = 20.7 mag with unprecedented precision. The ... [more ▼]

Context. Gaia is a space mission that is currently measuring the five astrometric parameters, as well as spectrophotometry of at least 1 billion stars to G = 20.7 mag with unprecedented precision. The sixth parameter in phase space (i.e., radial velocity) is also measured thanks to medium-resolution spectroscopy that is being obtained for the 150 million brightest stars. During the commissioning phase, two fields, one around each ecliptic pole, have been repeatedly observed to assess and to improve the overall satellite performances, as well as the associated reduction and analysis software. A ground-based photometric and spectroscopic survey was therefore initiated in 2007, and is still running to gather as much information as possible about the stars in these fields. This work is of particular interest to the validation of the radial velocity spectrometer outputs. <BR /> Aims: The paper presents the radial velocity measurements performed for the Southern targets in the 12-17 R magnitude range on high- to mid-resolution spectra obtained with the GIRAFFE and UVES spectrographs. <BR /> Methods: Comparison of the South Ecliptic Pole (SEP) GIRAFFE data to spectroscopic templates observed with the HERMES (Mercator in La Palma, Spain) spectrograph enabled a first coarse characterisation of the 747 SEP targets. Radial velocities were then obtained by comparing the results of three different methods. <BR /> Results: In this paper, we present an initial overview of the targets to be found in the 1 sq. deg SEP region that was observed repeatedly by Gaia ever since its commissioning. In our representative sample, we identified one galaxy, six LMC S-stars, nine candidate chromospherically active stars, and confirmed the status of 18 LMC Carbon stars. A careful study of the 3471 epoch radial velocity measurements led us to identify 145 RV constant stars with radial velocities varying by less than 1 km s[SUP]-1[/SUP]. Seventy-eight stars show significant RV scatter, while nine stars show a composite spectrum. As expected, the distribution of the RVs exhibits two main peaks that correspond to Galactic and LMC stars. By combining [Fe/H] and log g estimates, and RV determinations, we identified 203 members of the LMC, while 51 more stars are candidate members. <BR /> Conclusions: This is the first systematic spectroscopic characterisation of faint stars located in the SEP field. During the coming years, we plan to continue our survey and gather additional high- and mid-resolution data to better constrain our knowledge on key reference targets for Gaia. Tables 1-3, 5, 7, and 8 are only available at the CDS via anonym- ous ftp to <A href="http://cdsarc.u-strasbg.fr">http://cdsarc.u-strasbg.fr</A> (<A href="http://130.79.128.5">http://130.79.128.5</A>) or via <A href="http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/597/A10">http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/597/A10</A>Based on data taken with the VLT-UT2 of the European Southern Observatory, programmes 084.D-0427(A), 086.D-0295(A), and 088.D-0305(A).Based on data obtained from the ESO Science Archive Facility under request number 84886.Based on data obtained with the HERMES spectrograph, installed at the Mercator Telescope, operated on the island of La Palma by the Flemish Community, at the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias and supported by the Fund for Scientific Research of Flanders (FWO), Belgium, the Research Council of KU Leuven, Belgium, the Fonds National de la Recherche Scientifique (F.R.S.-FNRS), Belgium, the Royal Observatory of Belgium, the Observatoire de Genève, Switzerland and the Thüringer Landessternwarte Tautenburg, Germany. [less ▲]

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See detailLes nouveautés portées par la loi du 17 juin 2016 relative aux marchés publics, en particulier dans les secteurs spéciaux
Cariat, Nicolas; Vrancken, Martin ULg

in Revue du Droit des Industries de Réseau (2017), 1

La contribution offre un bref compte-rendu du nouveau régime mis en place par la loi du 17 juin 2016 relative aux marchés publics, transposant les directives 2014/24 et 2014/25, singulièrement dans les ... [more ▼]

La contribution offre un bref compte-rendu du nouveau régime mis en place par la loi du 17 juin 2016 relative aux marchés publics, transposant les directives 2014/24 et 2014/25, singulièrement dans les secteurs spéciaux. [less ▲]

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See detailEmployee perspectives on safety citizenship behaviors and safety violations
Chmiel, Nik; Laurent, Julie ULg; Hansez, Isabelle ULg

in Safety Science (2017), 93

Two studies investigate whether employees viewing discretionary safety activities as part of their job role (termed safety citizenship role definitions, SCRDs) plays an important part in predicting two ... [more ▼]

Two studies investigate whether employees viewing discretionary safety activities as part of their job role (termed safety citizenship role definitions, SCRDs) plays an important part in predicting two types of safety violation: routine violations conceptualized as related to an individual’s available cognitive energy or ‘effort’; and situational violations, which are those provoked by the organization (Reason, 1990). Study 1 showed SCRDs predicted situational violations only, and partially mediated the relationships between Perceived Management Commitment to Safety (PMCS) and work engagement with situational violations. These findings add to those by Hansez and Chmiel (2010), showing that routine and situational violations have predictors that differ. Study 1 findings also extend research reported by Turner et al. (2005), by showing that the effect of Job Control on SCRDs was mediated by both PMCS and work engagement. In study 2, participation in discretionary safety activities (safety participation) mediated the relationship between SCRDs and situational violations. Similar to study 1 The link between SCRDs and routine violations was non-significant and, strikingly, so was the link between safety participation and routine violations. These results support the view that processes involving SCRDs and safety participation are not cognitive-energetical in nature. In addition, study 2 findings extend previous work by Neal and Griffin (2006) by showing that SCRDs and safety knowledge partially mediated relationships between safety motivation and safety participation, whereas the direct effect of safety motivation on safety participation was non-significant. The results from both studies support the view that SCRDs are important in predicting situational violations. In study 2 SCRDs were shown to partially mediate the relationship between safety motivation and selfreported participation in discretionary safety activities (Safety Participation) which, in turn, related to situational violations. Interestingly there was no significant direct link between SCRDs and situational violations. These findings support the view that the effect of SCRDs on situational violations is fully mediated by participation in discretionary safety activities. [less ▲]

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See detailNumerical modelling of microscopic lubricant flow in sheet metal forming. Application to plane strip drawing
Carretta, Yves; Bech, Jacob; Legrand, Nicolas et al

in International Journal for Numerical Methods in Engineering (2017)

This paper presents a numerical investigation of microscopic lubricant flows from the cavities to the plateaus of the surface roughness of metal sheets during forming processes. This phenomenon, called ... [more ▼]

This paper presents a numerical investigation of microscopic lubricant flows from the cavities to the plateaus of the surface roughness of metal sheets during forming processes. This phenomenon, called micro-plastohydrodynamic lubrication, was observed experimentally in various situations such as compression sliding tests, strip drawing and cold rolling. It leads to local friction drop and wear reduction. It is therefore critical to achieve a good understanding of this phenomenon. As to move towards that goal, a multiscale fluid–structure interaction model is developed to model lubricant flows at the microscopic scale. These simulations are made possible through the use of the Arbitrary Lagrangian Eulerian (ALE) formalism. In this paper, this methodology is used to study plane strip drawing. The numerical model is able to predict the onset of lubricant escape and the amount of lubricant flowing on the plateaus. Numerical results exhibit good agreement with experimental measurements. [less ▲]

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See detailEFHC1 variants in juvenile myoclonic epilepsy: reanalysis according to NHGRI and ACMG guidelines for assigning disease causality
Bailey, JN; Patterson, C; de Nijs, L et al

in Genetics in Medicine (2017)

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See detailVers de nouvelles pistes diagnostiques et thérapeutiques pour l'arthrose chez l'homme
Boulocher, Caroline; Henrotin, Yves ULg

in Le nouveau praticien vétérinaire canine/féline (2017), 14(65), 7-10

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See detailComparison of Indium Tin Oxide and Indium Tungsten Oxide as Transparent Conductive Substrates for WO3-Based Electrochromic Devices
Maho, Anthony ULg; Nicolay, Sylvain; Manceriu, Laura ULg et al

in Journal of the Electrochemical Society (2017), 164(2), 25-31

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See detailP-301 High Concentration Multistrain Probiotic Produced at Different Manufacturing Sites: Comparative Analysis.
Lombardi, Francesca; La Torre, Cristina; Giusti, Ilaria et al

in Inflammatory Bowel Diseases (2017), 23

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See detailComputerized adaptive testing with R: Recent updates of the package catR
Magis, David ULg; Barrada, Juan Ramon

in Journal of Statistical Software (2017), 76(1), 1-19

The purpose of this paper is to list the recent updates of the R package catR. This package allows for generating response patterns under a computerized adaptive testing (CAT) framework with underlying ... [more ▼]

The purpose of this paper is to list the recent updates of the R package catR. This package allows for generating response patterns under a computerized adaptive testing (CAT) framework with underlying item response theory (IRT) models. Among the most important updates, well-known polytomous IRT models are now supported by catR; several item selection rules have been added; and it is now possible to perform post-hoc simulations. Some functions were also rewritten or withdrawn to improve the usefulness and performances of the package. [less ▲]

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See detailImpacts of the Hara Biosphere Reserve on Livelihood and Welfare in Persian Gulf
Dehghani Pour, Milad; Motiee, Naser; Akbar Barati, Ali et al

in Ecological Economics (2017), 141(November 2017), 7686

Despite the importance of biosphere reserves in Iran's livelihood and welfare, the economic significance of Hara Biosphere Reserve has never been comprehensively studied. This study examines the current ... [more ▼]

Despite the importance of biosphere reserves in Iran's livelihood and welfare, the economic significance of Hara Biosphere Reserve has never been comprehensively studied. This study examines the current importance of Hara Biosphere Reserve (HBR) in local livelihood and welfare. Using a household survey, data were collected through a questionnaire, key informant interviews and direct observations. Two hundred and forty-four households were randomly selected from 10 villages through proportional sampling. Results showed that non-environmental income was the first driver of the total income, poverty alleviation and narrowing income inequality gap. Park income was the second. The results also showed that excluding park income from total income would significantly increase headcount poverty, widen the poverty gap, and raise the Gini coefficient. Wealthier households had the greatest absolute income from the environment, including forest, fishing and fodder. However, the poorest group had smallest absolute income from these sources. Thus, the study demonstrated that wealthier households are responsible for the overharvesting of environmental resources. Interestingly, the study showed that wealthier households are more dependent on profitable environmental incomes sources while the poorest are more dependent on non-profitable ones. [less ▲]

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See detailSwimmer's Itch in Belgium: First Recorded Outbreaks, Molecular Identification of the Parasite Species and Intermediate Hosts.
Caron, Yannick ULg; Cabaraux, Ariane; Marechal, Francoise et al

in Vector borne and zoonotic diseases (Larchmont, N.Y.) (2017)

BACKGROUND: Cercarial dermatitis or swimmer's itch is a skin condition in humans due to the larval forms of bird schistosomes of some species of the genus Trichobilharzia. The life cycle of these ... [more ▼]

BACKGROUND: Cercarial dermatitis or swimmer's itch is a skin condition in humans due to the larval forms of bird schistosomes of some species of the genus Trichobilharzia. The life cycle of these schistosomes requires freshwater snails (intermediate host) and waterfowl (definitive host). Repeated exposures to cercariae can lead to skin sensitization with the induction of pruritic skin lesions. METHODS: We describe, in this study, two outbreaks of human cercarial dermatitis at the Eau d'Heure Lakes, Belgium. In July and August 2012, a total of, respectively, 78 and 10 people reported a sudden skin rash accompanied by pruritus following recreational activities in the Plate Taille Lake. However, no ocellate furcocercariae were detected following light exposure of the snails collected between September 2012 and September 2013 (n = 402). No outbreaks were recorded in 2013 and 2014. In August 2015, about 30 new cases were recorded. Snails were collected (n = 270) in different locations around the lake. PCR was used to identify accurately the intermediate hosts and the parasite species involved. RESULTS: After light exposure, seven Radix spp. (2.6%) shed ocellate furcocercariae. Molecular identification based on the rDNA internal transcribed spacer 2 sequence ascribed the infected snails to R. balthica ( = R. peregra = R. ovata) (6/7) and R. auricularia (1/7). Based on the amplification of the D2 domain of the 28S rDNA, the cercariae were shown to belong to two different haplotypes of Trichobilharzia franki. CONCLUSIONS: This is the first record in Belgium of T. franki and associated skin condition. [less ▲]

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See detailSpaces Snu, diametral dimension and property Omega bar
Demeulenaere, Loïc ULg

in Journal of Mathematical Analysis and Applications (2017), 449(2), 1340-1350

This paper investigates two topological invariants in the context of the sequence spaces Snu, which are metric topological vector spaces defined in order to study the regularity of signals. First, it ... [more ▼]

This paper investigates two topological invariants in the context of the sequence spaces Snu, which are metric topological vector spaces defined in order to study the regularity of signals. First, it extends the formula already known of the diametral dimension of spaces Snu to some non-locally pseudoconvex ones. Second, it proves that locally p-convex spaces Snu verifies the property "Omega id", which is equivalent to Omega bar for Fréchet spaces. [less ▲]

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See detailLa géographie linguistique au nord du domaine d'oïl
Baiwir, Esther ULg

in Bien Dire et Bien Aprandre : Bulletin du Centre d'Etudes Médiévales et Dialectales de l'Université de Lille III (2017), 32

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See detailAnxiety modulates the relation between attention- deficit / hyperactivity disorder severity and working memory-related brain activity
Meer, Dennis Van Der; Hoekstra, Pieter J.; Rooij, Daan Van et al

in World Journal of Biological Psychiatry (2017), 0(0), --000

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See detailHigh resolution (1 km) positive degree-day modelling of Greenland ice sheet surface mass balance, 1870–2012 using reanalysis data
Wilton, D.; Jowett, A.; Hanna, E. et al

in Journal of Glaciology (2017), 63

We show results from a positive degree-day (PDD) model of Greenland ice sheet (GrIS) surface mass balance (SMB), 1870–2012, forced with reanalysis data. The model includes an improved daily temperature ... [more ▼]

We show results from a positive degree-day (PDD) model of Greenland ice sheet (GrIS) surface mass balance (SMB), 1870–2012, forced with reanalysis data. The model includes an improved daily temperature parameterization as compared with a previous version and is run at 1 km rather than 5 km resolution. The improvements lead overall to higher SMB with the same forcing data. We also compare our model with results from two regional climate models (RCMs). While there is good qualitative agreement between our PDD model and the RCMs, it usually results in lower precipitation and lower runoff but approximately equivalent SMB: mean 1979–2012 SMB (± standard deviation), in Gt a−1, is 382 ± 78 in the PDD model, compared with 379 ± 101 and 425 ± 90 for the RCMs. Comparison with in situ SMB observations suggests that the RCMs may be more accurate than PDD at local level, in some areas, although the latter generally compares well. Dividing the GrIS into seven drainage basins we show that SMB has decreased sharply in all regions since 2000. Finally we show correlation between runoff close to two calving glaciers and either calving front retreat or calving flux, this being most noticeable from the mid-1990s. [less ▲]

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See detailLa modification du biais attentionnel dans les troubles anxieux et la dépression : une revue synthétique de la littérature
Blairy, Sylvie ULg

in Annales Médico-Psychologiques (2017), 175(6), 522-527

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See detailOptimized approach to retrieve information on atmospheric carbonyl sulfide (OCS) above the Jungfraujoch station and change in its abundance since 1995
Lejeune, Bernard ULg; Mahieu, Emmanuel ULg; Vollmer, M. K. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (2017), 186

In this paper, we present an optimized retrieval strategy for carbonyl sulfide (OCS), using Fourier transform infrared (FTIR) solar observations made at the high-altitude Jungfraujoch station in the Swiss ... [more ▼]

In this paper, we present an optimized retrieval strategy for carbonyl sulfide (OCS), using Fourier transform infrared (FTIR) solar observations made at the high-altitude Jungfraujoch station in the Swiss Alps. More than 200 lines of the nu3 fundamental band of OCS have been systematically evaluated and we selected 4 microwindows on the basis of objective criteria minimizing the effect of interferences, mainly by solar features, carbon dioxide and water vapor absorption lines, while maximizing the information content. Implementation of this new retrieval strategy provided an extended time series of the OCS abundance spanning the 1995-2015 time period, for the study of the long-term trend and seasonal variation of OCS in the free troposphere and stratosphere. Three distinct periods characterize the evolution of the tropospheric partial columns: a first decreasing period (1995-2002), an intermediate increasing period (2002-2008), and the more recent period (2008-2015) which shows no significant trend. Our FTIR tropospheric and stratospheric time series are compared with new in situ gas chromatography mass spectrometry (GCMS) measurements performed by Empa (Laboratory for Air Pollution/Environmental Technology) at the Jungfraujoch since 2008, and with space-borne solar occultation observations by the ACE-FTS instrument on-board the SCISAT satellite, respectively, and they show good agreement. The OCS signal recorded above Jungfraujoch appears to be closely related to anthropogenic sulfur emissions. [less ▲]

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See detailComparison of clinical effects of epidural levobupivacaine morphine versus bupivacaine morphine in dogs undergoing elective pelvic limb surgery
Cerasoli, Ilaria; Tutunaru, Alexandru-Cosmin ULg; Cenani, Alessia et al

in Veterinary Anaesthesia & Analgesia (2017)

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See detailThe diplomacy of practitioners: for an ecology of practices about the problem of the coexistence of wind farms and red kites
Melard, François ULg; Stassart, Pierre M ULg

in Environmental Education Research (2017)

Gathering information, comparing points of view, and designing actions in and about problematic situations are no longer purely academic activities. Learning about complex state of a airs is becoming an ... [more ▼]

Gathering information, comparing points of view, and designing actions in and about problematic situations are no longer purely academic activities. Learning about complex state of a airs is becoming an increasingly widely- distributed necessity and practice, including within civil society. We propose to give rst-hand information – as practitioners and members of a citizens’ wind energy co-operative in Belgium – about the problematic and highly controversial coexistence between wind farms and red kite (Milvus milvus). Will be reported the implementation of a community-based management scheme involving active, original practices of collaboration between citizens with local knowledge and skills and experts or scientists in order to produce research and intervention questions, methods, and results that are claimed to be more sustainable. The sustainability challenges are to design and set up in context a local space for collaboration and learning that departs from traditional public regulatory procedures (i.e. impact studies, public information meetings, etc.). However, the outcomes of this collaborative approach remain fragile. We shall argue in this regard that both the Transdisciplinary Environmental Research and the art of diplomacy may be central to the emergence of re exive governance within transition processes that strive to be sustainable. [less ▲]

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See detailThermal Conductivity of Straw Bales: Full Size Measurements Considering the Direction of the Heat Flow
Costes, Jean-Philippe; Evrard, Arnaud; Biot, Benjamin et al

in Buildings (2017), 7(11), 1-15

The thermal conductivity of straw bales is an intensively discussed topic in the international straw bale community. Straw bales are, by nature, highly heterogeneous and porous. They can have a relatively ... [more ▼]

The thermal conductivity of straw bales is an intensively discussed topic in the international straw bale community. Straw bales are, by nature, highly heterogeneous and porous. They can have a relatively large range of density and the baling process can influence the way the fibres are organised within the bale. In addition, straw bales have a larger thickness than most of the insulating materials that can be found in the building industry. Measurement apparatus is usually not designed for such thicknesses, and most of the thermal conductivity values that can be found in the literature are defined based on samples in which the straw bales are resized. During this operation, the orientation of the fibres and the density may not be preserved. This paper starts with a literature review of straw bale thermal conductivity measurements and presents a measuring campaign performed with a specific Guarded Hot Plate, designed to measure samples up to 50 cm thick. The influence of the density is discussed thoroughly. Representative values are proposed for a large range of straw bales to support straw-bale development in the building industry. [less ▲]

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See detailThe use of oxytocin to improve feeding and social skills in infants with Prader-Willi syndrome
Tauber, Maïthé; Boulanouar, Kader; Gwenaelle, Diene et al

in Pediatrics (2017), 139(2),

BACKGROUND AND OBJECTIVES: Patients with Prader–Willi syndrome (PWS) display poor feeding and social skills as infants and fewer hypothalamic oxytocin (OXT)-producing neurons were documented in adults ... [more ▼]

BACKGROUND AND OBJECTIVES: Patients with Prader–Willi syndrome (PWS) display poor feeding and social skills as infants and fewer hypothalamic oxytocin (OXT)-producing neurons were documented in adults. Animal data demonstrated that early treatment with OXT restores sucking after birth. Our aim is to reproduce these data in infants with PWS. METHODS: We conducted a phase 2 escalating dose study of a short course (7 days) of intranasal OXT administration. We enrolled 18 infants with PWS under 6 months old (6 infants in each step) who received 4 IU of OXT either every other day, daily, or twice daily. We investigated the tolerance and the effects on feeding and social skills and changes in circulating ghrelin and brain connectivity by functional MRI. RESULTS: No adverse events were reported. No dose effect was observed. Sucking assessed by the Neonatal Oral-Motor Scale was abnormal in all infants at baseline and normalized in 88% after treatment. The scores of Neonatal Oral-Motor Scale and videofluoroscopy of swallowing significantly decreased from 16 to 9 (P < .001) and from 18 to 12.5 (P < .001), respectively. Significant improvements in Clinical Global Impression scale scores, social withdrawal behavior, and mother–infant interactions were observed. We documented a significant increase in acylated ghrelin and connectivity of the right superior orbitofrontal network that correlated with changes in sucking and behavior. CONCLUSIONS: OXT is well tolerated in infants with PWS and improves feeding and social skills. These results open perspectives for early treatment in neurodevelopment diseases with feeding problems. [less ▲]

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See detailThe impact of automated hippocampal volumetry on diagnostic confidence in patients with suspected Alzheimer's disease: an EADC study
Bosco, P.; Redolfi, A.; Bocchetta, M. et al

in Alzheimer's & Dementia : The Journal of the Alzheimer's Association (2017)

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See detailUse of the potential probiotic strain Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers.
Saint-Cyr, Manuel Jimmy; Haddad, Nabila; Taminiau, Bernard ULg et al

in International Journal of Food Microbiology (2017)

Campylobacteriosis is the most frequently reported zoonotic disease in humans in the EU since 2005. As chicken meat is the main source of contamination, reducing the level of Campylobacter in broiler ... [more ▼]

Campylobacteriosis is the most frequently reported zoonotic disease in humans in the EU since 2005. As chicken meat is the main source of contamination, reducing the level of Campylobacter in broiler chicken will lower the risk to consumers. The aim of this project was to evaluate the ability of Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers and to investigate the mechanisms that could be involved. Thirty broilers artificially contaminated with C. jejuni were treated by oral gavage with MRS broth or a bacterial suspension (107CFU) of Lb. salivarius SMXD51 (SMXD51) in MRS broth. At 14 and 35days of age, Campylobacter and Lb. salivarius loads were assessed in cecal contents. The impact of the treatment on the avian gut microbiota at day 35 was also evaluated. At day 14, the comparison between the control and treated groups showed a significant reduction (P<0.05) of 0.82 log. After 35days, a significant reduction (P<0.001) of 2.81 log in Campylobacter loads was observed and 73% of chickens treated with the culture exhibited Campylobacter loads below 7log10CFU/g. Taxonomic analysis revealed that SMXD51 treatment induced significant changes (P<0.05) in a limited number of bacterial genera of the avian gut microbiota and partially limited the impact of Campylobacter on Anaerotruncus sp. decrease and Subdoligranulum sp. increase. Thus, SMXD51 exhibits an anti-Campylobacter activity in vivo and can partially prevent the impact of Campylobacter on the avian gut microbiota. [less ▲]

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See detailNuclear Motion Driven Ultrafast Photodissociative Charge Transfer of the PENNA Cation: An Experimental and Computational Study
Sun, Shoutian; Mignolet, Benoît ULg; Fan, lin et al

in Journal of Physical Chemistry A (2017)

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See detailA dynamic, sex-specific expression pattern of genes regulating thyroid hormone action in the developing zebra finch song control system
Raymaekers, S.R.; Verbeure, W.; Ter Haar, S.M. et al

in General and Comparative Endocrinology (2017), 240

The zebra finch (Taeniopygia guttata) song control system consists of several series of interconnected brain nuclei that undergo marked changes during ontogeny and sexual development, making it an excel ... [more ▼]

The zebra finch (Taeniopygia guttata) song control system consists of several series of interconnected brain nuclei that undergo marked changes during ontogeny and sexual development, making it an excel- lent model to study developmental neuroplasticity. Despite the demonstrated influence of hormones such as sex steroids on this phenomenon, thyroid hormones (THs) – an important factor in neural devel- opment and maturation – have not been studied in this regard. We used in situ hybridization to compare the expression of TH transporters, deiodinases and receptors between both sexes during all phases of song development in male zebra finch. Comparisons were made in four song control nuclei: Area X, the lateral magnocellular nucleus of the anterior nidopallium (LMAN), HVC (used as proper name) and the robust nucleus of the arcopallium (RA). Most genes regulating TH action are expressed in these four nuclei at early stages of development. However, while general expression levels decrease with age, the activating enzyme deiodinase type 2 remains highly expressed in Area X, HVC and RA in males, but not in females, until 90days post-hatch (dph), which marks the end of sensorimotor learning. Furthermore, the L-type amino acid transporter 1 and TH receptor beta show elevated expression in male HVC and RA respectively compared to surrounding tissue until adulthood. Differences compared to sur- rounding tissue and between sexes for the other TH regulators were minor. These developmental changes are accompanied by a strong local increase in vascularization in the male RA between 20 and 30 dph but not in Area X or HVC. Our results suggest that local regulation of TH signaling is an important factor in the development of the song control nuclei during the song learning phase and that TH activation by DIO2 is a key player in this process. [less ▲]

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See detailRecovery of fibers and biomethane from banana peduncles biomass through anaerobic digestion
AwedemWobiwo, Florent; Korangi Alleluya, Virginie; Happi Emaga, Thomas et al

in Energy for Sustainable Development (2017), 37

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See detailIncreased expression of bacterial amoA during an N2O emission peak in an agricultural field
Theodorakopoulos, Nicolas ULg; Lognoul, Margaux ULg; Degrune, Florine ULg et al

in Agriculture, Ecosystems & Environment (2017), 236

Levels of N-cycle gene transcripts (nirK, nirS, nosZ, amoA) were measured during an N2O emission peak in an agricultural soil. Automated dynamic closed chambers were used to monitor an N2O emission peak ... [more ▼]

Levels of N-cycle gene transcripts (nirK, nirS, nosZ, amoA) were measured during an N2O emission peak in an agricultural soil. Automated dynamic closed chambers were used to monitor an N2O emission peak on a maize crop after a natural rainfall. The peak occurred rapidly after the rainfall began. Spatial and temporal variability in N2O emission was observed between chambers. An analysis of N-cycle gene transcript levels revealed an increase in bacterial amoA gene transcripts (but not in archaeal amoA transcripts), correlating strongly with N2O emission. This suggests the involvement of nitrification enzymes, despite a high water-filled pore space (80%). Reverse transcription of bacterial 16S rRNA followed by partial sequencing of the resulting cDNAs revealed few rainfall-induced changes in the potentially active bacterial community, and notably no significant change in the relative abundance of 16S rRNAs from the nitrifier genus Nitrosospira. Expression of the amoA gene appears as a possible proxy for monitoring the N2O emission peak. To our knowledge, this is the first experiment to evaluate the expression of N-cycle genes during an N2O emission peak on an agricultural field. [less ▲]

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See detailInterpreting ‘grief’ in Senegal: language, emotions and cross-cultural translation in a francophone African context
Ruth, Evans; Jane; Fatou, Kebe et al

in Mortality (2017)

This article reflects on the profound complexities of translating and interpreting ‘grief’, and emotions and responses to death more broadly, in multilingual, cross-cultural contexts. Drawing on ... [more ▼]

This article reflects on the profound complexities of translating and interpreting ‘grief’, and emotions and responses to death more broadly, in multilingual, cross-cultural contexts. Drawing on qualitative research conducted in urban Senegal, West Africa, we discuss the exchange of meanings surrounding grief and death through language, including the process of translation, in its broadest sense, between multiple languages (Wolof, French, English). Our experiences demonstrate the crucial importance of involving interpreters and field researchers throughout the research process, to gain fundamental insight into the cultural nuances of indigenous languages and how these are translated and potentially re-framed in the process. We reflect on our iterative process of discussing emerging interpretations with participants in follow-up workshops and with our interpreter. This approach helped shed light on language use surrounding ‘grief’ and how this is bound up with wider socio-cultural norms which make particular emotions surrounding death and experiences/meanings of death and bereavement possible and ‘speak-able’. Our research calls for greater recognition in death and bereavement studies of the cultural specificity of conceptual frameworks developed in minority European socio-linguistic contexts and demonstrates the need for greater engagement with theoretical, empirical and methodological insights gained in diverse cultural contexts in the Majority world. [less ▲]

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See detailTracking the Subtle Mutations Driving Host Sensing by the Plant Pathogen Streptomyces scabies
Jourdan, Samuel; Francis, Jourdan; Deflandre, Benoit et al

in mSphere (2017)

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See detailHyperinsulinemia and elevated systolic blood pressure independently predict white matter hyperintensities with associated cognitive decrement in the middle-aged offspring of dementia patients.
Hawkins, Keith A.; Emadi, Nazli; Pearlson, Godfrey D. et al

in Metabolic Brain Disease (2017)

Cerebrovascular disease is an independent risk factor for dementia that may also be synergistic with Alzheimer's disease. In recent years attention has switched from cerebral infarcts to microvascular ... [more ▼]

Cerebrovascular disease is an independent risk factor for dementia that may also be synergistic with Alzheimer's disease. In recent years attention has switched from cerebral infarcts to microvascular disease as the primary cause of cerebrovascular cognitive decline, with damage to the white matter the primary mechanism. Uncertainties remain regarding the risks posed by different types vascular threat, the extent to which cerebrovascular damage occurs in middle age, and whether relatively "normal" amounts of white matter damage are accompanied by meaningful degrees of cognitive decline. We explored these issues via laboratory, cardiovascular, cognitive, and magnetic resonance imaging (MRI) data in 67 middle-aged cognitively normal offspring of dementia patients. The sample was enriched for vascular risk. Plasma insulin, 24-h systolic blood pressure, body mass index, age, and % small dense LDL cholesterol were the strongest correlates of MRI white matter hyperintensity (WMH) volume. With shared variance controlled for, 24 h systolic BP, plasma insulin, and age remained as significant predictors of WMH volume. An interaction variable (24 h BP * insulin) did not improve the prediction of WMH. WMH volume correlated negatively with cognition. No evidence for an ApoE epsilon4 effect emerged for either WMH or cognition. Hypertension and hyperinsulinemia appear to pose independent, consequential threats to the cerebral small vessel vasculature in middle age, reflected in the presence of areas of WMH on MRI scans. Our data show that even modest WMH volumes in middle age are associated with cognitive decrement, underscoring the importance of aggressive treatment and lifestyle modifications to address vascular risk throughout adulthood. [less ▲]

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See detailRetos actuales en la caracterización de los flujos agua-aire en grandes obras hidráulicas
Valero Huerta, Daniel ULg

in REVISTA HIDROLATINOAMERICANA (2017), 1

Present literature on air-water flows is briefly reviewed, including articles of the main five international Journals published along the last four years. Main advances in the most fruitful topics related ... [more ▼]

Present literature on air-water flows is briefly reviewed, including articles of the main five international Journals published along the last four years. Main advances in the most fruitful topics related to air entrainment are discussed. Main areas of interest are: stepped spillways, bubbles in pipe flows, numerical modelling and instrumentation. [less ▲]

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See detailUse of donor milk in the neonatal intensive care unit
de halleux, Virginie; Pieltain, Catherine; SENTERRE, Thibault ULg et al

in Seminars in Fetal & Neonatal Medicine (2017)

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See detailGuidelines for the Detection of Babesia and Theileria Parasites.
Lempereur, Laetitia ULg; Beck, Relja; Fonseca, Isabel et al

in Vector borne and zoonotic diseases (Larchmont, N.Y.) (2017), 17(1), 51-65

The genera Babesia and Theileria (phylum Apicomplexa, order Piroplasmida) are mainly transmitted by Ixodid ticks in which the sexual part of their life cycle followed by sporogony takes place. They ... [more ▼]

The genera Babesia and Theileria (phylum Apicomplexa, order Piroplasmida) are mainly transmitted by Ixodid ticks in which the sexual part of their life cycle followed by sporogony takes place. They include protozoan parasites that infect erythrocytes of a variety of vertebrate hosts, including domestic and wild animals, with some Babesia spp. also infecting humans. Babesia sporozoites transmitted in the tick's saliva during the bloodmeal directly infect erythrocytes, where they asexually multiply to produce pear-shaped merozoites in the process of merogony; whereas a pre-erythrocytic schizogonic life stage in leukocytes is found in Theileria and precedes merogony in the erythrocytes. The wide spectrum of Babesia and Theileria species and their dissimilar characteristics with relation to disease severity, transmission, epidemiology, and drug susceptibility stress the importance of accurate detection of babesiosis and theileriosis and their causative agents. These guidelines review the main methods currently used for the detection of Babesia and Theileria spp. for diagnostic purposes as well as epidemiological studies involving their vertebrate hosts and arthropod vectors. Serological methods were not included once they did not indicate current infection but rather exposure. [less ▲]

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See detailFactors controlling spatial and temporal patterns of multiple pesticide compounds in groundwater (Hesbaye chalk aquifer, Belgium)
Hakoun, Vivien; Orban, Philippe ULg; Dassargues, Alain ULg et al

in Environmental Pollution (2017)

Factors governing spatial and temporal patterns of pesticide compounds (pesticides and metabolites) concentrations in chalk aquifers remain unclear due to complex flow processes and multiple sources. To ... [more ▼]

Factors governing spatial and temporal patterns of pesticide compounds (pesticides and metabolites) concentrations in chalk aquifers remain unclear due to complex flow processes and multiple sources. To uncover which factors govern pesticide compound concentrations in a chalk aquifer, we develop a methodology based on time series analyses, uni- and multivariate statistics accounting for concentrations below detection limits. The methodology is applied to long records (1996–2013) of a restricted compound (bentazone), three banned compounds (atrazine, diuron and simazine) and two metabolites (deethylatrazine (DEA) and 2,6–dichlorobenzamide (BAM)) sampled in the Hesbaye chalk aquifer in Belgium. In the confined area, all compounds had non-detects fractions >80%. By contrast, maximum concentrations exceeded EU’s drinking-water standard (100 ngL-1) in the unconfined area. This contrast confirms that recent recharge and polluted water did not reach the confined area, yet. Multivariate analyses based on variables representative of the hydrogeological setting revealed higher diuron and simazine concentrations in the southeast of the unconfined area, where urban activities dominate land use and where the aquifer lacks protection from a less permeable layer of hardened chalk. At individual sites, positive correlations (up to τ =0,48 for bentazone) between pesticide compound concentrations and multi-annual groundwater level fluctuations confirm occurrences of remobilization. A downward temporal trend of atrazine concentrations likely reflects decreasing use of this compound over the last 28 years. However, the lack of a break in concentrations time series and maximum concentrations of atrazine, simazine, DEA and BAM exceeding EU’s standard post-ban years provide evidence of persistence. Contrasting upward trends in bentazone concentrations show that a time lag is required for restriction measures to be efficient. These results shed light on factors governing pesticide compound concentrations in chalk aquifers. The developed methodology is not restricted to chalk aquifers, it could be transposed to study other pollutants with concentrations below detection limits. Several factors govern pesticide compounds concentrations in the chalk: hydrogeological setting, land use, groundwater level fluctuations and persistence. [less ▲]

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See detailInfrared imaging of high density protein arrays.
De Meutter, Joelle; Vandenameele, Julie ULg; Matagne, André ULg et al

in Analyst (2017)

We propose in this paper that protein microarrays could be analysed by infrared imaging in place of enzymatic or fluorescence labelling. This label-free method reports simultaneously a large series of ... [more ▼]

We propose in this paper that protein microarrays could be analysed by infrared imaging in place of enzymatic or fluorescence labelling. This label-free method reports simultaneously a large series of data on the spotted sample (protein secondary structure, phosphorylation, glycosylation, presence of impurities, etc.). In the present work, 100 mum protein spots each containing about 100 pg protein were deposited to form high density regular arrays. Using arrays of infrared detectors, high resolution images could be obtained where each pixel of the image is in fact a full infrared spectrum. With microarrays, hundreds of experimental conditions can be tested easily and quickly, with no further labelling or chemistry of any kind. We describe how the noise present in the infrared spectra can be split into image noise and detector noise. We also detail how both types of noise can be most conveniently dealt with to generate very high quality spectra of less than 100 pg protein. Finally, the results suggest that the protein secondary structure is preserved during microarray building. [less ▲]

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See detailThe right dorsal premotor mosaic: organization, functions, and connectivity
Genon, Sarah ULg; Li, Hai; Fan, Lingzhong et al

in Cerebral Cortex (2017), 27(3), 2095-2110

The right dorsal premotor cortex (PMd) of humans has been reported to be involved in a broad range of motor and cognitive functions. We explored the basis of this behavioral heterogeneity by performing a ... [more ▼]

The right dorsal premotor cortex (PMd) of humans has been reported to be involved in a broad range of motor and cognitive functions. We explored the basis of this behavioral heterogeneity by performing a connectivity-based parcellation using meta-analytic approach applied to PMd coactivations. We compared our CBP results to parcellations obtained through resting-state functional connectivity and probabilistic diffusion tractography,. Functional connectivity profiles and behavioral decoding of the resulting PMd subregions allowed characterizing their respective behavior profile. These procedures divided the right PMd into five distinct subregions that formed a cognitive-motor gradient along a rostro-caudal axis. In particular, we found (i) a rostral subregion functionally connected with prefrontal cortex, which likely supports high-level cognitive processes, such as working memory (ii) a central subregion showing a mixed behavioral profile and functional connectivity to parietal regions of the dorsal attention network, and (iii) a caudal subregion closely integrated with the motor system. Additionally, we found (iv) a dorsal subregion, preferentially related to hand movements and connected to both cognitive and motor regions, and (v) a ventral subregion, whose functional profile fits the concept of an eye-movement related field. In conclusion, right PMd may be considered as a functional mosaic formed by five subregions. [less ▲]

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See detailLa délimitation du champ des activités exclusivement sociales dans la jurisprudence de la Cour de justice de l’Union européenne : essai de clarification
Detienne, Quentin ULg

in Revue de Droit Social = Tijdschrift voor Sociaal Recht (2017), (1), 331-364

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See detailRange size heritability and diversification patterns in the liverwort genus Radula
Patiño, J.; Wang, J.; Renner, M.A.M. et al

in Molecular Phylogenetics & Evolution (2017), 106

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See detailEditor's Choice - Management of Descending Thoracic Aorta Diseases: Clinical Practice Guidelines of the European Society for Vascular Surgery (ESVS).
Riambau, V.; Bockler, D.; Brunkwall, J. et al

in European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery (2017), 53(1), 4-52

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See detailEmission and oxidation of methane in a meromictic, eutrophic and temperate lake (Dendre, Belgium)
Roland, Fleur ULg; Darchambeau, François ULg; Morana, Cédric ULg et al

in Chemosphere (2017)

We sampled the water column of the Dendre stone pit lake (Belgium) in spring, summer, autumn and winter. Depth profiles of several physico-chemical variables, nutrients, dissolved gases (CO2, CH4, N2O ... [more ▼]

We sampled the water column of the Dendre stone pit lake (Belgium) in spring, summer, autumn and winter. Depth profiles of several physico-chemical variables, nutrients, dissolved gases (CO2, CH4, N2O), sulfate, sulfide, iron and manganese concentrations and d13C-CH4 were determined. We performed incubation experiments to quantify CH4 oxidation rates, with a focus on anaerobic CH4 oxidation (AOM), without and with an inhibitor of sulfate reduction (molybdate). The evolution of nitrate and sulfate concentrations during the incubations was monitored. The water column was anoxic below 20 m throughout the year, and was thermally stratified in summer and autumn. High partial pressure of CO2 and CH4 and high concentrations of ammonium and phosphate were observed in anoxic waters. Important nitrous oxide and nitrate concentration maxima were also observed (up to 440 nmol L- 1 and 80 mmol L -1, respectively). Vertical profiles of d13C-CH4 unambiguously showed the occurrence of AOM. Important AOM rates (up to 14 mmol L -1 d- 1) were observed and often co-occurred with nitrate consumption peaks, suggesting the occurrence of AOM coupled with nitrate reduction. AOM coupled with sulfate reduction also occurred, since AOM rates tended to be lower when molybdate was added. CH4 oxidation was mostly aerobic (~80% of total oxidation) in spring and winter, and almost exclusively anaerobic in summer and autumn. Despite important CH4 oxidation rates, the estimated CH4 fluxes from the water surface to the atmosphere were high (mean of 732 mmol m- 2 d- 1 in spring, summer and autumn, and up to 12,482 mmol m- 2 d- 1 in winter). [less ▲]

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See detailTime related effects on functional brain connectivity after serotonergic and cholinergic neuromodulation.
Klaassens, Bernadet L.; Rombouts, Serge A. R. B.; Winkler, Anderson ULg et al

in Human Brain Mapping (2017), 38(1), 308-325

Psychopharmacological research, if properly designed, may offer insight into both timing and area of effect, increasing our understanding of the brain's neurotransmitter systems. For that purpose, the ... [more ▼]

Psychopharmacological research, if properly designed, may offer insight into both timing and area of effect, increasing our understanding of the brain's neurotransmitter systems. For that purpose, the acute influence of the selective serotonin reuptake inhibitor citalopram (30 mg) and the acetylcholinesterase inhibitor galantamine (8 mg) was repeatedly measured in 12 healthy young volunteers with resting state functional magnetic resonance imaging (RS-fMRI). Eighteen RS-fMRI scans were acquired per subject during this randomized, double blind, placebo-controlled, crossover study. Within-group comparisons of voxelwise functional connectivity with 10 functional networks were examined (P < 0.05, FWE-corrected) using a non-parametric multivariate approach with cerebrospinal fluid, white matter, heart rate, and baseline measurements as covariates. Although both compounds did not change cognitive performance on several tests, significant effects were found on connectivity with multiple resting state networks. Serotonergic stimulation primarily reduced connectivity with the sensorimotor network and structures that are related to self-referential mechanisms, whereas galantamine affected networks and regions that are more involved in learning, memory, and visual perception and processing. These results are consistent with the serotonergic and cholinergic trajectories and their functional relevance. In addition, this study demonstrates the power of using repeated measures after drug administration, which offers the chance to explore both combined and time specific effects. Hum Brain Mapp 38:308-325, 2017. (c) 2016 Wiley Periodicals, Inc. [less ▲]

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See detailUnderstanding and overcoming metformin gastrointestinal intolerance.
Bonnet, Fabrice; Scheen, André ULg

in Diabetes, Obesity & Metabolism (2017), 19(473-481),

Metformin is the most widely prescribed drug for patients with type 2 diabetes mellitus and the first-line pharmacologic option as supported by multiple international guidelines, yet a rather large ... [more ▼]

Metformin is the most widely prescribed drug for patients with type 2 diabetes mellitus and the first-line pharmacologic option as supported by multiple international guidelines, yet a rather large proportion of patients cannot tolerate metformin in adequate amounts because of its associated gastrointestinal (GI) adverse events (AEs). GI AEs typically encountered with metformin therapy include diarrhoea, nausea, flatulence, indigestion, vomiting, and abdominal discomfort, with diarrhoea and nausea being the most common. Although starting at a low dose and titrating slowly may help prevent some of metformin's GI AEs, some patients are unable to tolerate metformin at all and it may also be difficult to convince patients to start metformin again after a bout of GI AEs. Despite this clinical importance the underlying mechanisms of metformin's GI intolerance are poorly known. This review discusses the epidemiology of metformin GI intolerance; its underlying mechanisms; genotype variability and associated factors affecting metformin GI intolerance, such as comorbidities, co-medications, and bariatric surgery; clinical consequences, and therapeutic strategies to overcome metformin GI intolerance. These strategies include appropriate titration of immediate-release metformin, use of extended-release metformin, the promise of delayed-release metformin and gut microbiome modulators, and alternative pharmacological therapies when metformin cannot be tolerated at all. Given the available data, all efforts should be made to maintain metformin before considering a shift to another drug therapy. [less ▲]

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See detailComputation of GPS P1–P2 Differential Code Biases with JASON-2
Wautelet, Gilles ULg; Loyer, Sylvain; Mercier, Flavien et al

in GPS Solutions (2017)

GPS Differential Code Biases (DCBs) computation is usually based on ground networks of permanent stations. The drawback of the classical methods is the need for the ionospheric delay so that any error in ... [more ▼]

GPS Differential Code Biases (DCBs) computation is usually based on ground networks of permanent stations. The drawback of the classical methods is the need for the ionospheric delay so that any error in this quantity will map into the solution. Nowadays, many low-orbiting satellites are equipped with GPS receivers which are initially used for precise orbitography. Considering spacecrafts at an altitude above the ionosphere, the ionized contribution comes from the plasmasphere, which is less variable in time and space. Based on GPS data collected onboard JASON-2 spacecraft, we present a methodology which computes in the same adjustment the satellite and receiver DCBs in addition to the plasmaspheric vertical total electron content (VTEC) above the satellite, the average satellite bias being set to zero. Results show that GPS satellite DCB solutions are very close to those of the IGS analysis centers using ground measurements. However, the receiver DCB and VTEC are closely correlated, and their value remains sensitive to the choice of the plasmaspheric parametrization. [less ▲]

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See detailIntegration of order picking and vehicle routing in a B2C e-commerce context
Moons, Stef; Ramaekers, Katrien; Caris, An et al

in Flexible Services and Manufacturing Journal (2017)

E-commerce sales are increasing every year and customers who buy goods on the Internet have high service level expectations. In order to meet these expectations, a company’s logistics operations need to ... [more ▼]

E-commerce sales are increasing every year and customers who buy goods on the Internet have high service level expectations. In order to meet these expectations, a company’s logistics operations need to be performed carefully. Optimizing only internal warehouse processes will often lead to suboptimal solutions. The interrelationship between the order picking process and the delivery process should not be ignored. Therefore, in this study, an order picking problem and a vehicle routing problem with time windows and release dates are solved simultaneously using a single optimization framework. To the best of our knowledge, it is the first time that an order picking problem and a vehicle routing problem are integrated. A mixed integer linear programming formulation for this integrated order picking-vehicle routing problem (OP-VRP) is provided. The integrated OPVRP is solved for small instances and the results are compared to these of an uncoordinated approach. Computational experiments show that integration can lead to cost savings of 14% on average. Furthermore, higher service levels can be offered by allowing customers to request their orders later and still get delivered within the same time windows. [less ▲]

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See detailFoam fractionation as a tool to study the air-water interfacestructure-function relationship of wheat gluten hydrolysates
Wouters, Arno G.B.; Rombouts, Ine; Schoebrechts, Nele et al

in Colloids and Surfaces B : Biointerfaces (2017), 151

Enzymatic hydrolysis of wheat gluten protein improves its solubility and produces hydrolysates withfoaming properties which may find applications in food products. First, we here investigated whetherfoam ... [more ▼]

Enzymatic hydrolysis of wheat gluten protein improves its solubility and produces hydrolysates withfoaming properties which may find applications in food products. First, we here investigated whetherfoam-liquid fractionation can concentrate wheat gluten peptides with foaming properties. Foam andliquid fractions had high and very low foam stability (FS), respectively. In addition, foam fractions wereable to decrease surface tension more pronouncedly than un-fractionated samples and liquid fractions,suggesting they are able to arrange themselves more efficiently at an interface. As a second objective,foam fractionation served as a tool to study the structural properties of the peptides, causing thesedifferences in air-water interfacial behavior. Zeta potential and surface hydrophobicity measurements didnot fully explain these differences but suggested that hydrophobic interactions at the air-water interfaceare more important than electrostatic interactions. RP-HPLC showed a large overlap between foam andliquid fractions. However, a small fraction of very hydrophobic peptides with relatively high averagemolecular mass was clearly enriched in the foam fraction. These peptides were also more concentratedin un-fractionated DH 2 hydrolysates, which had high FS, than in DH 6 hydrolysates, which had low FS.These peptides most likely play a key role in stabilizing the air-water interface. [less ▲]

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See detailEquine Atypical Myopathy in the UK: Epidemiological characteristics of cases reported from 2011 to 2015 and factors associated with survival
Gonzalez-Medina, S.; Ireland, J. L.; Piercy, R. J. et al

in Equine Veterinary Journal (2017)

BACKGROUND: Equine atypical myopathy (AM) is a toxic rhabdomyolysis associated with ingestion of hypoglycin A, derived typically in Europe, from Acer pseudoplatanus tree. Despite the wide distribution of ... [more ▼]

BACKGROUND: Equine atypical myopathy (AM) is a toxic rhabdomyolysis associated with ingestion of hypoglycin A, derived typically in Europe, from Acer pseudoplatanus tree. Despite the wide distribution of this tree species in the UK, the number of cases reported annually varies, and there has been an apparent increase in prevalence in recent years. Although AM was first recognised in the UK, epidemiological studies have never been conducted focused solely on this country. OBJECTIVES: To describe the spatiotemporal distribution, presentation, treatment and outcome of AM cases reported in the UK. STUDY DESIGN: Retrospective case series. METHODS: British AM cases reported to the atypical myopathy alert website, between 2011 and 2015 were included (n = 224). Data were obtained via standardised epidemiological questionnaires from owners and veterinarians. Factors associated with survival were assessed using logistic regression. RESULTS: Most cases reported were from England (87.9%). Survival was 38.6% (n = 73/189). Clinical factors associated with reduced odds of survival included, hypothermia (odds ratio (OR) 0.18; CI 0.06-0.57; p = 0.01), bladder distension (OR 0.11; CI 0.02-0.59; p = 0.01), tachycardia (OR 0.97; CI 0.94-0.99; p = 0.04) and serum creatine kinase activity >100,000 IU/L (OR 0.17; CI 0.04-0.68; p = 0.01) in the univariable analysis as well as recumbency. The latter was the only sign retained in multivariable analysis (OR = 0.19; CI 0.06-0.62; p = 0.006). Administration of vitamins during the disease was associated with survival (OR 3.75; CI 1.21-11.57; p = 0.02). MAIN LIMITATIONS: Reporting cases to the atypical myopathy alert group is voluntary; therefore, under-reporting will result in underestimation of AM cases; furthermore, direct owner-reporting could have introduced misdiagnosis bias. CONCLUSION: Some areas of the UK reported AM cases more commonly. Clinical signs such as recumbency, rectal temperature, distended bladder and serum CK activity might be useful prognostic indicators though should be considered in the context of the clinical picture. Treatment with vitamins increases survival. This article is protected by copyright. All rights reserved. [less ▲]

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See detailLong-Term Care Insurance and Intra-family Moral Hazard: Fixed vs Proportional Insurance Benefits
Klimaviciute, Justina ULg

in GENEVA Risk & Insurance Review (2017)

Pauly (1990) argues that an explanation for the low long-term care (LTC) insurance demand could be intra-family moral hazard: parents might refuse to buy insurance since it reduces children’s incentives ... [more ▼]

Pauly (1990) argues that an explanation for the low long-term care (LTC) insurance demand could be intra-family moral hazard: parents might refuse to buy insurance since it reduces children’s incentives to provide care. This paper raises and explores the idea that the extent of intra-family moral hazard and non-purchase of LTC insurance might differ when insurance benefits are fixed and when they are proportional to LTC expenditures. It shows that fixed benefits limit and might even eliminate intra-family moral hazard, while the effect of proportional benefits is at best ambiguous. Consequently, non-purchase of insurance is less likely with fixed benefits. [less ▲]

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See detailThe role of short-term memory and visuo-spatial skills in numerical magnitude processing: evidence from Turner syndrome
Attout, Lucie ULg; Noël, Marie-Pascale; Nassogne, Marie-Cécile et al

in PLoS ONE (2017), 12(2), 0171454

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See detailThe Tourist on the farm: a 'muddled' image
Dubois, Charline ULg; Cawley, Mary; Schmitz, Serge ULg

in Tourism Management (2017), 59

Agritourism is promoted as a method of offsetting decline in farm incomes, and is defined in different ways which creates difficulties in attracting tourists. The present research contributes to a better ... [more ▼]

Agritourism is promoted as a method of offsetting decline in farm incomes, and is defined in different ways which creates difficulties in attracting tourists. The present research contributes to a better understanding of agritourism, based on evidence in Wallonia (Belgium) and Luxembourg. It does so by studying the image held by four groups of actors: (i) farmers who provide ‘agritourist’ experiences; (ii) promotional organisations; and tourists who both (iii) have and (iv) have not engaged in agritourism. Agritourism is a muddled concept between realities and stakeholder expectations but opportunities exist for targeting particular niche interests. [less ▲]

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See detailThe role of mesenchymal stem cells in the treatment of ulcerative colitis and Crohn's disease
GREGOIRE, Céline ULg; Louis, Edouard ULg; BRIQUET, Alexandra ULg et al

in The Biology and Therapeutic Application of Mesenchymal Cells (2017)

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See detailHow does forest cover impact water flows and ecosystem services? Insights from real-life catchments in Wallonia (Belgium)
Brogna, Delphine; Vincke, Caroline; Brostaux, Yves ULg et al

in Ecological Indicators (2017), 72

While planet boundaries are being crossed and ecosystems degraded, the Ecosystem Service (ES) conceptrepresents a potential decision-making tool for improved natural resources management. The main aimof ... [more ▼]

While planet boundaries are being crossed and ecosystems degraded, the Ecosystem Service (ES) conceptrepresents a potential decision-making tool for improved natural resources management. The main aimof this paper is to assess the impact of forest cover on water related ES in Wallonia (Belgium) in termsof quantity and timing. We developed an approach based on easily accessible data, monitored in severalcountries and using straightforward statistical methods. This led us to study ES at “real-life” catchmentsscale: 22 catchments – from 30 to 250 km2– with mixed land covers were studied. We approached thewater supply and flood protection services through 5 indicators extracted from 10 hydrological years(2005–2014) discharge data series. These were computed annually and seasonally (vegetation periodfrom March to September and “non-vegetation” period the rest of the year). The water supply wasassessed through the specific volume Vs, the baseflow index BFI and the specific discharge exceeded95% of the time Q95s whereas the flood protection service was approached through the specific dis-charge exceeded 5% of the time Q05s and the flashiness index FI. Our study gives two main insights. First,statistical analyses show that forest cover negatively impact water supply when studying annual and“non-vegetation” period flows in general (Vs) but positively when studying low flows (Q95s). Regardingflood protection a slightly negative impact of forest cover on high flows (Q05s) was highlighted in the“non-vegetation” period. Results also show a negative impact of forests annually and in the vegetationperiod on the flashy behaviour of the catchment thus a positive impact on the flood protection ES. The“year” effect is overall highly significant testifying the importance of climatic factors. Rainfall is oftensignificant and can be considered as a main driver of these ES. Secondly, analyzing the quality of themodels produced and the results overall we assume that other variables characterizing the catchmentssuch as topography or soil types do play a significant role in the delivery of these ES. This questions theuse of land cover proxies in assessing and mapping of hydrological ES at a complex landscape scale. Wethus recommend further research to keep improving land cover proxies if they are used. [less ▲]

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See detailScènes médiatiques et arènes de discours - Formes d'engagement dans un monde perdu
Servais, Christine ULg

in Réseaux : Communication - Technologie - Société (2017), 202-203

Cet article interroge l’efficacité politique de deux dispositifs (Site de « Riposte laïque » et page Facebook de « Français de Souche ») dans le cadre de la distinction entre « conflit agonistique » et « ... [more ▼]

Cet article interroge l’efficacité politique de deux dispositifs (Site de « Riposte laïque » et page Facebook de « Français de Souche ») dans le cadre de la distinction entre « conflit agonistique » et « conflit antagoniste » proposée par Chantal Mouffe : quelle est la nature du collectif auquel ils font appel et qu’ils configurent ? Cette question ne peut être élucidée sans que soit examinée la relation entre ces dispositifs médiatiques et leurs récepteurs. Il s’agit donc d’analyser, par un travail sur les formes énonciatives, la scène sur laquelle apparaissent les collectifs d’extrême-droite, les formes d’engagements que cette scène mobilise et ses manières spécifiques de construire un « nous », caractérisées par la perte d’objets communs. [less ▲]

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See detailAccuracy and performance of continuous glucose monitors in athletes
Thomas, Felicity Louise ULg; Pretty, Christopher; Signal, Matthew et al

in Biomedical Signal Processing and Control (2017)

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See detailEvidence of the impact of visuo-spatial processing on magnitude representation in 22q11.2 microdeletion syndrome.
Attout, Lucie ULg; Noël, Marie-Pascale; Vossius, Line ULg et al

in Neuropsychologia (2017), 99

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See detailQuadratic reformulations of nonlinear binary optimization problems
Anthony, Martin; Boros, Endre; Crama, Yves ULg et al

in Mathematical Programming (2017), 162

Very large nonlinear unconstrained binary optimization problems arise in a broad array of applications. Several exact or heuristic techniques have proved quite successful for solving many of these ... [more ▼]

Very large nonlinear unconstrained binary optimization problems arise in a broad array of applications. Several exact or heuristic techniques have proved quite successful for solving many of these problems when the objective function is a quadratic polynomial. However, no similarly efficient methods are available for the higher degree case. Since high degree objectives are becoming increasingly important in certain application areas, such as computer vision, various techniques have been recently developed to reduce the general case to the quadratic one, at the cost of increasing the number of variables. In this paper we initiate a systematic study of these quadratization approaches. We provide tight lower and upper bounds on the number of auxiliary variables needed in the worst-case for general objective functions, for bounded-degree functions, and for a restricted class of quadratizations. Our upper bounds are constructive, thus yielding new quadratization procedures. Finally, we completely characterize all ``minimal'' quadratizations of negative monomials. [less ▲]

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See detailPlasma effects on atomic data for the K-vacancy states of highly charged iron ions
Deprince, J.; Fritzsche, S.; Kallman, T.R. et al

in AIP Conference Proceedings (2017), 1811

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See detailComparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species
Kapepula, Paulin Mutwale; Kabamba Ngombe, Nadege; Tshisekedi Tshibangu, Pascal et al

in Natural Product Research (2017), 6419(March), 1--8

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See detailEnergy and nutrient content of food served and consumed by nursing home residents
Buckinx, Fanny ULg; ALLEPAERTS, Sophie ULg; PAQUOT, Nicolas ULg et al

in the journal of nutrition, health & aging (2017), 21(6), 727-32

Objective: The aim of this study was to compare energy and protein content of the served food with the actual intake from the food consumed by nursing home residents. This study also aimed to compare food ... [more ▼]

Objective: The aim of this study was to compare energy and protein content of the served food with the actual intake from the food consumed by nursing home residents. This study also aimed to compare food intake and dietary allowances. Design: This is a cross sectional study. Setting: This study was performed in nursing homes. Participants: Residents of these 2 nursing homes were eligible for the study if they agreed to participate and if they meet the selection criteria (to be older than 65 years and have a regular texture diet). Measurement: Nutrient content of the served food and real food consumption was calculated for all meals during a 5-day period by precise weighting method. Difference between consumed and served dietary content was evaluated by the Chi² test. Results: Seventy-four Belgian nursing home residents (75% of women, 85.8 ± 7.04 years on average) were included in this study. These subjects had a mean body mass index of 24.9 ± 4.83 kg/m². The mean energy content of the served food was 1783.3 ± 125.7 kcal per day. However, residents did not eat the whole of the meals and the actual energy content of the consumed food was significantly less (1552.4 ± 342.1 kcal per day; p<.001). The average protein content of the food served was equal to 0.96 ± 0.20 g/kg/day and the average consumption of protein by the residents was 0.88 ± 0.25 g/kg/day. The difference between protein served and consumed was also significant (p=.04). Moreover, people considered as well nourished, eating significantly more energy than the others (p=.04). Conclusion: Meals served in nursing homes are not entirely consumed by their residents. As expected, the energy consumed are lower in subjects considered as malnourished or at risk of malnutrition. [less ▲]

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See detailInterleukin 13-mediated colitis in the absence of IL-4Rα signalling
Hoving, Jennifer C; Cutler, Antony J; Leeto, Mosiuoa et al

in Gut (2017)

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See detailFactors associated with self-perceived burden to the primary caregiver in older patients with hematologic malignancies: an exploratory study
Libert, Y; Borghgraef, C; BEGUIN, Yves ULg et al

in Psycho-oncology (2017), 26

Objective: Although cancer patients frequently experience self-perceived burden to others, this perception has not been enough studied. The aim of this study was to investigate the prevalence of ... [more ▼]

Objective: Although cancer patients frequently experience self-perceived burden to others, this perception has not been enough studied. The aim of this study was to investigate the prevalence of selfperceived burden to the primary caregiver (SPB-PC) and associated factors in an older patient population with hematologic malignancies at the time of chemotherapy initiation. Methods: In total, 166 consecutive patients with hematologic malignancies aged ≥65 years were recruited at the time of chemotherapy initiation. Patients’ SPB-PC was assessed using a 100-mm visual analogue scale (VAS). Characteristics potentially associated with SPB-PC, including sociodemographic and medical characteristics, physical functioning status (Karnofsky performance score, activities of daily living (ADL)/instrumental ADL), symptoms (fatigue, pain, nausea, quality of life), psychological distress (Hospital Anxiety and Depression Scale (HADS)), perceived cognitive function (Functional Assessment of Cancer Therapy Cognitive (FACT-Cog) Scale), and patients’/primary caregivers’ personal relationship characteristics (family tie, support), were assessed. Results: Thirty-five percent of patients reported moderate to severe SPB-PC (VAS ≥ 50 mm). Patients’ SPB-PC was associated with lower Karnofsky performance (β = 0.135, p = 0.058) and ADL (β = 0.148, p = 0.037) scores, and higher HADS (β = 0.283, p<0.001) and FACT-Cog perceived cognitive impairments subscale (β = 0.211, p = 0.004) scores. The proportion of explained variance was 23.5%. Conclusions: Health care professionals should be aware that about one third of older cancer patients experience moderate to severe SPB-PC at the time of chemotherapy initiation. They should adapt their support of patients who report such a feeling. [less ▲]

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See detailAnalysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Franciosi, Federica; Tessario, Irene; Dalbies-Tran, Rozenn et al

in Journal of Visualized Experiments (2017), 123(e55242),

The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of ... [more ▼]

The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice. Specifically, the chromosomes and the centromeres of metaphase II (MII) oocytes were fluorescently labeled and counted on sequential focal plans after confocal laser microscope scanning. This analysis revealed a higher incidence in the aneuploidy rate when immature oocytes were collected from the follicles and matured in vitro compared to in vivo. Immunostaining for tubulin and the acetylated form of histone four at specific lysine residues also revealed differences in the morphology of the meiotic spindle and in the global pattern of histone acetylation. Finally, the expression of mRNAs coding for histone deacetylases (HDACs) and acetyl-transferases (HATs) was investigated by reverse transcription and quantitative-PCR (q-PCR). No differences in the relative expression of transcripts were observed between in vitro and in vivo matured oocytes. In agreement with a general silencing of the transcriptional activity during oocyte maturation, the analysis of the total transcript amount can only reveal mRNA stability or degradation. Therefore, these findings indicate that other translational and post-translational regulations might be affected. Overall, the present study describes an experimental approach to morphologically and biochemically characterize the horse oocyte, a cell type that is extremely challenging to study due to low sample availability. However, it can expand our knowledge on the reproductive biology and infertility in monovulatory species. [less ▲]

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See detailInfusion of mesenchymal stromal cells after deceased liver transplantation: A phase I-II, open-label, clinical study.
DETRY, Olivier ULg; VANDERMEULEN, Morgan ULg; DELBOUILLE, Marie-Hélène ULg et al

in Journal of Hepatology (2017)

BACKGROUND & AIM: Mesenchymal stromal cell (MSC) infusion could be a mean to establish tolerance in solid organ recipients. The aim of this prospective, controlled, phase-1 study was to evaluate the ... [more ▼]

BACKGROUND & AIM: Mesenchymal stromal cell (MSC) infusion could be a mean to establish tolerance in solid organ recipients. The aim of this prospective, controlled, phase-1 study was to evaluate the feasibility, safety and tolerability of a single infusion of MSCs in liver transplant recipients. METHODS: Ten liver transplant recipients under standard immunosuppression received 1.5-3x106/kg third-party unrelated MSCs on post-operative day 3+/-2, and were prospectively compared to a control group of 10 liver transplant recipients. As primary end-points, MSC infusional toxicity was evaluated, and infectious and cancerous complications were prospectively recorded until month 12 in both groups. As secondary end-points, rejection rate, month-6 graft biopsies, and peripheral blood lymphocyte phenotyping were compared. Progressive immunosuppression weaning was attempted from month 6 to 12 in MSC recipients. RESULTS: No variation in vital parameters or cytokine release syndrome could be detected during and after MSC infusion. No patient developed impairment of organ functions (including liver graft function) following MSC infusion. No increased rate of opportunistic infection or de novo cancer was detected. As secondary end-points, there was no difference in overall rates of rejection or graft survival. Month-6 biopsies did not demonstrate a difference between groups in the evaluation of rejection according to the Banff criteria, in the fibrosis score or in immunohistochemistry (including Tregs). No difference in peripheral blood lymphocyte typing could be detected. The immunosuppression weaning in MSC recipients was not successful. CONCLUSIONS: No side effect of MSC infusion at day 3 after liver transplant could be detected, but this infusion did not promote tolerance. This study opens the way for further MSC or Treg-based trials in liver transplant recipients. LAY SUMMARY: Therapy with mesenchymal stromal cells (MSCs) has been proposed as a mean to improve results of solid organ transplantation. One of the potential MSC role could be to induce tolerance after liver transplantation, i.e. allowing the cessation of several medications with severe side effects. This study is the first-in-man use of MSC therapy in 10 liver transplant recipients. This study did not show toxicity after a single MSC infusion but it was not sufficient to allow withdrawal of immunosuppression. [less ▲]

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See detailStructural models of the different trimers present in the core of phycobilisomes from Gracilaria chilensis based on crystal structures and sequences.
Dagnino-Leone, Jorge; Figueroa, Maximiliano; Mella, Claudia et al

in PLoS ONE (2017), 12(5), 0177540

Phycobilisomes (PBS) are accessory light harvesting protein complexes that directionally transfer energy towards photosystems. Phycobilisomes are organized in a central core and rods radiating from it ... [more ▼]

Phycobilisomes (PBS) are accessory light harvesting protein complexes that directionally transfer energy towards photosystems. Phycobilisomes are organized in a central core and rods radiating from it. Components of phycobilisomes in Gracilaria chilensis (Gch) are Phycobiliproteins (PBPs), Phycoerythrin (PE), and Phycocyanin (PC) in the rods, while Allophycocyanin (APC) is found in the core, and linker proteins (L). The function of such complexes depends on the structure of each component and their interaction. The core of PBS from cyanobacteria is mainly composed by cylinders of trimers of alpha and beta subunits forming heterodimers of Allophycocyanin, and other components of the core including subunits alphaII and beta18. As for the linkers, Linker core (LC) and Linker core membrane (LCM) are essential for the final emission towards photoreaction centers. Since we have previously focused our studies on the rods of the PBS, in the present article we investigated the components of the core in the phycobilisome from the eukaryotic algae, Gracilaria chilensis and their organization into trimers. Transmission electron microscopy provided the information for a three cylinders core, while the three dimensional structure of Allophycocyanin purified from Gch was determined by X-ray diffraction method and the biological unit was determined as a trimer by size exclusion chromatography. The protein sequences of all the components of the core were obtained by sequencing the corresponding genes and their expression confirmed by transcriptomic analysis. These subunits have seldom been reported in red algae, but not in Gracilaria chilensis. The subunits not present in the crystallographic structure were modeled to build the different composition of trimers. This article proposes structural models for the different types of trimers present in the core of phycobilisomes of Gch as a first step towards the final model for energy transfer in this system. [less ▲]

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See detailKey impact of an uncommon plasmid on bacillus amyloliquefaciens subsp. plantarum S499 developmental traits and lipopeptide production
Molinatto, G.; Franzil, L.; Steels, Sébastien ULg et al

in Frontiers in Microbiology (2017), 8(JAN),

The rhizobacterium Bacillus amyloliquefaciens subsp. plantarum S499 (S499) is particularly efficient in terms of the production of cyclic lipopeptides, which are responsible for the high level of plant ... [more ▼]

The rhizobacterium Bacillus amyloliquefaciens subsp. plantarum S499 (S499) is particularly efficient in terms of the production of cyclic lipopeptides, which are responsible for the high level of plant disease protection provided by this strain. Sequencing of the S499 genome has highlighted genetic differences and similarities with the closely related rhizobacterium B. amyloliquefaciens subsp. plantarum FZB42 (FZB42). More specifically, a rare 8008 bp plasmid (pS499) harboring a rap-phr cassette constitutes a major distinctive element between S499 and FZB42. By curing this plasmid, we demonstrated that its presence is crucial for preserving the typical physiology of S499 cells. Indeed, the growth rate and extracellular proteolytic activity were significantly affected in the cured strain (S499 P-). Furthermore, pS499 made a significant contribution to the regulation of cyclic lipopeptide production. Surfactins and fengycins were produced in higher quantities by S499 P-, whereas lower amounts of iturins were detected. In line with the increase in surfactin release, bacterial motility improved after curing, whereas the ability to form biofilm was reduced in vitro. The antagonistic effect against phytopathogenic fungi was also limited for S499 P-, most probably due to the reduction of iturin production. With the exception of this last aspect, S499 P- behavior fell between that of S499 and FZB42, suggesting a role for the plasmid in shaping some of the phenotypic differences observed in the two strains. © 2017 Molinatto, Franzil, Steels, Puopolo, Pertot and Ongena. [less ▲]

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See detail3D echocardiographic reference ranges for normal left ventricular volumes and strain: results from the EACVI NORRE study
BERNARD, Anne-Catherine ULg; Addetia, K; DULGHERU, Raluca Elena ULg et al

in European Heart Journal - Cardiovascular Imaging (2017)

Aim: To obtain the normal ranges for 3D echocardiography (3DE) measurement of left ventricular (LV) volumes, function, and strain from a large group of healthy volunteers. Methods and results: A total of ... [more ▼]

Aim: To obtain the normal ranges for 3D echocardiography (3DE) measurement of left ventricular (LV) volumes, function, and strain from a large group of healthy volunteers. Methods and results: A total of 440 (mean age: 45 ± 13 years) out of the 734 healthy subjects enrolled at 22 collaborating institutions of the Normal Reference Ranges for Echocardiography (NORRE) study had good-quality 3DE data sets that have been analysed with a vendor-independent software package allowing homogeneous measurements regardless of the echocardiographic machine used to acquire the data sets. Upper limits of LV end-diastolic and end-systolic volumes were larger in men (97 and 42 mL/m2) than in women (82 and 35 mL/m2; P < 0.0001). Conversely, lower limits of LV ejection fraction were higher in women than in men (51% vs. 50%; P < 0.01). Similarly, all strain components were higher in women than in men. Lower range was -18.6% in men and -19.5% in women for 3D longitudinal strain, -27.0% and -27.6% for 3D circumferential strain, -33.2% and -34.4% for 3D tangential strain and 38.8% and 40.7% for 3D radial strain, respectively. LV volumes decreased with age in both genders (P < 0.0001), whereas LV ejection fraction increased with age only in men. Among 3DE LV strain components, the only one, which did not change with age was longitudinal strain. Conclusion: The NORRE study provides applicable 3D echocardiographic reference ranges for LV function assessment. Our data highlight the importance of age- and gender-specific reference values for both LV volumes and strain. [less ▲]

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