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See detailOviposition deterring effect of Ocimum basilicum L. (Lamiaceae) on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)
Yarou, Boni Barthélémy ULg; Bawin, Thomas ULg; Boullis, Antoine ULg et al

Poster (2016, December 02)

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic ... [more ▼]

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yield in greenhouses and fields, in several countries around the world. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and producers’ health, there is a need for alternatives. In this study, we assess the oviposition deterring effect of O. basilicum using dual-choice behavioral assays performed in flight tunnels. Two modalities were tested, i.e. (1) a tomato plant associated either with an O. basilicum plant versus a tomato plant associated with a plastic pot only filled with potting soil, and (2) a tomato plant with basil essential oil (EO) formulated in paraffin oil (PO) versus a tomato plant with PO only. A 1 ml cylindrical polyethylene plug loaded with 100 µl of solution (formulated EO or PO) was placed on each plant as a diffuser. Forty-eight hours after the release of unsexed adult individuals randomly sampled from the rearing in the central area of the tunnel, we found that plants and EOs reduced T. absoluta oviposition behavior on a nearby located tomato plant. GC-MS analyses showed that the major constituents include estragol (73.8%), linalool (8.6%), β-elemene (2.9%) in O. basilicum essential oils and E-α-bergamotene (38.9%), methyl eugenol (26.1 %), E-β-ocimene (17.7 % ) in O. basilicum VOCs collected with solid-phase micro-extraction method. These results suggest a valuable potential of O. basilicum and associated essential oils as component of an integrated management strategy against the tomato leafminer. [less ▲]

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See detailGeneralized Pascal triangles and binomial coefficients of words
Stipulanti, Manon ULg

Poster (2016, December 01)

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word ... [more ▼]

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a word appears as a subsequence of another finite word. Similarly to the Sierpinski gasket that can be built as the limit set, for the Hausdorff distance, of a convergent sequence of normalized compact blocks extracted from Pascal triangle modulo 2, we describe and study the first properties of the subset of [0, 1] × [0, 1] associated with this extended Pascal triangle modulo a prime p. From the extended Pascal triangle obtained when p is equal to 2, we derive a sequence of which we study the regularity and the asymptotic behavior of the summatory function. Inspired from this regularity, we extend our results to another famous numeration systems, namely the Zeckendorff numeration system. [less ▲]

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See detailMobility of 137Cs, 10Be and 210Pb in soil as a function of the soil texture, organic matter content and pH
de Tombeur, Félix ULg; Cornu, Sophie; Bourlès, Didier et al

Poster (2016, November 30)

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ... [more ▼]

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ecosystem services. In order to better constrain and quantify these processes, a kinetic quantification of the involved transfers based on vertical profiles of 210Pb (xs), 10Be and 137Cs has been developed for Luvisols in which soluble transfers could be neglected. The range of applicability of this type of approach was nevertheless not tested. In this work, we analyzed 210Pb (xs), 10Be and 137Cs in soils exhibiting gradients of organic matter, pH and < 2 μm fraction (Podzol, Andosol Ferralsol, Leptosol). Acidic soils and large organic matter contents were considered. The depth distribution of the studied isotopes was then interpreted as a function of the organic matter content, the soil pH and its < 2 μm fraction. This demonstrates that 10Be and 137Cs losses occur under soluble form in the Podzol, especially under forest, more acid, for the latter. Soluble 10Be transfers were also identified in the Andosol when they were considered as negligible in the Leptosol. For 210Pb (xs), soluble transfers are also probable for soils with pH less than 5.5 (Andosol and Ferralsol) and strong affinity for organic matter has been demonstrated. Thus, under acidic conditions and low < 2 μm fraction, soluble transfer cannot neglected anymore. [less ▲]

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See detailL'activation comportementale est-elle pertinente pour tous les adultes dépressifs ? Une analyse en clusters
Wagener, Aurélie ULg; Baeyens, Céline; Van der Linden, Martial ULg et al

Poster (2016, November 26)

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression ... [more ▼]

INTRODUCTION : L’hétérogénéité de la dépression est de plus en plus mise en évidence en termes de profils de symptômes, de réponse au traitement, etc. Les théories comportementales de la dépression soulignent l’importance de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes dans le maintien de la dépression. Cette étude poursuit deux objectifs : (1) investiguer l’hétérogénéité de la depression via une analyse en clusters, (2) investiguer les valeurs prédictives de l’inactivité ainsi que des faibles niveaux de plaisir et de fréquence d’activités plaisantes sur la dépression en fonction du cluster d’appartenance. METHODE : Des adultes tout venant et/ou consultant des centres de santé mentale ont été recrutés (âge entre 18 et 60 ans). Ils ont complété l’Inventaire de Dépression de Beck – Seconde Edition, l’Echelle d’Activation Comportementale pour la Dépression – Version courte, et l’Inventaire des événements plaisants. RESULTATS : Une solution en six clusters a été mise en évidence : “gros dormeurs/heavy sleepers”, “dépressifs cognitifs/cognitive depressives”, “dépressifs affectifs-somatiques/affective-somatic depressives”, “dépressifs légers/mild depressives”, “dépressifs dormant peu/sleepless depressives”, “dépressifs typiques/typical depressives”. Des relations significatives entre inactivité, fréquence d’activités plaisantes et dépression ont seulement été observées chez les “dépressifs cognitifs/cognitive depressives”, les “dépressifs légers/mild depressives” et les “dépressifs typiques/typical depressives”. DISCUSSION : Les résultats confirment l’hétérogénéité de la dépression puisque six clusters ont été mis en évidence. Par ailleurs, l’activation comportementale ne semble pertinente que pour trois de ces clusters dans lesquels les postulats du modèle comportemental de la dépression sont confirmés (“dépressifs cognitifs/cognitive depressives”, “dépressifs légers/mild depressives”, “dépressifs typiques/typical depressives”). [less ▲]

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See detailHot-Melt Extrusion as a Continuous Manufacturing Process to Form Ternary Cyclodextrin Inclusion Complexes
Thiry, Justine ULg; Krier, Fabrice; Ratwatte, Shenelka et al

Poster (2016, November 16)

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See detailZinc, iron and manganese interaction within the frd3 Arabidopsis mutant
Scheepers, Maxime ULg; Spielmann, Julien ULg; Goormaghtigh, Erik et al

Poster (2016, November 16)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. Arabidopsis halleri is a zinc- and cadmium-tolerant and zinc-hyperaccumulating species, which also present adaptation of its iron homeostasis(1,4). Transcriptomic studies identified genes which are constitu-tively over-expressed in Arabidopsis halleri compared to Arabidopsis thaliana and which may have a role in metal tolerance or accumulation(2-4). Among them, a candidate gene encodes the FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters. FRD3 is a citrate transporter involved in iron homeostasis(5-7) and plays a role in zinc tolerance in A. thaliana(8). The FRD3 gene displays a complex regulation. In A. thaliana, alternative transcript initiation for FRD3 determines two transcripts, which dif-fer in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence(9). In A. halleri, a single highly ex-pressed FRD3 transcript with high translation efficiency is present(9). [less ▲]

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See detailDevelopment of a new method for arabidopsides extraction and purification
Genva, Manon ULg; Andersson, Mats X; Nasir, Mehmet Nail ULg et al

Poster (2016, November 16)

In the present work, a new method for arabidopsides extraction and purification was developed.

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See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, November 16)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells. We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood. : We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells. [less ▲]

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See detailElektrodynamische Fragmentierung von Elektroschrott
Iseli, Cédric; Streicher-Porte, Martin; Martino, Rémi ULg et al

Poster (2016, November 11)

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden ... [more ▼]

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden kann. Verschweisste Kompaktgeräte können zum Beispiel so weit geöffnet werden, dass die ge-wünschte Komponente freigelegt und entfernt werden kann. Leiterplatten haben die Eigenschaft, dass bei kontinuierlich steigendem Energieeintrag zuerst die aufgebrachten Komponenten abgetrennt werden, bevor die Cu-Bahnen freigelegt werden. Bei noch höherem Energieeintrag wird die Leiterplatte schlussendlich zerkleinert und vollständig zer-stört. Durch diese Entfrachtung der Leiterplatte kann ein metallreiches Konzentrat hergestellt werden. [less ▲]

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See detailEndocrine disruptors and parasitism: which effects on the sex behaviour of the amphipod Gammarus pulex ?
Fivet, Adeline; Horion, Sibylle; Thomé, Jean-Pierre ULg et al

Poster (2016, November 10)

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See detailSevere deficiency of the somatotrope GHRH/GH/IGF-1 axis induces a dramatic susceptibility to Streptococcus pneumoniae infection.
Farhat, Khalil ULg; Bodart, Gwennaëlle ULg; Desmet, Christophe ULg et al

Poster (2016, November 07)

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation ... [more ▼]

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation (Alba & Salvatori, Endocrinology 2004;145:4134). In basal conditions, the immunological phenotype of Ghrh-/- mice is not markedly disturbed except for a decrease in B cells and an increase in generation of thymic (t) Treg cells (submitted for publication). These data prompted us to investigate immune responses of Ghrh-/- mice using vaccination and infection by S. pneumoniae as models since the response to both stimuli rely on the innate immune system and B cells. Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH differently impacts on B-1, marginal zone B-2 or innate B-1 B cells. Furthermore, after intranasal instillation of a non-lethal dose of serotype 1 S. pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility reflected by bacteremia 24h after infection and a survival limit of 72 h, compared to WT C57BL/6 mice that need only 24h to clear infection. The possible impact of GH deficiency on components of the innate immune system that play an important role in defense of the respiratory tract against pneumococcal infection is under current investigation. (*Equal first and last authors. KF is supported by a research grant from the Lebanese Government; GB is Research Assistant, CD is Research Associate, and VG is Research Director at the NFSR of Belgium). [less ▲]

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See detailRecent evolution of the coastline in the Gulf of Guinea. Example of Togo and Benin (2000-2015)
Ozer, Pierre ULg; Hountondji, Yvon-Carmen; de Longueville, Florence

Poster (2016, November 04)

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See detail« Cap-Haïtien » or « how to ‘construct’ a flood risk in a decade »
Gracius, Gracia Joseph; Ozer, Pierre ULg

Poster (2016, November 04)

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See detailRisk management in Ivory Coast : Case Study of Population Evictions in Port-Bouët, Abidjan
Comoe, Raymond; Ozer, Pierre ULg

Poster (2016, November 04)

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See detailTerminologia Multilíngue de Clínica General / Medicina de Família e Comunidade
Jamoulle, Marc ULg; Treuherz, Arthur; Gomes, Luis Filipe et al

Poster (2016, November 02)

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently ... [more ▼]

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently addressed by GPs when they meet in congresses. A taxonomy has been build. A multilingual terminological record has been developed for each of the 182 items identified. This work is freely available online on www.hetop.eu/Q (free access after inscription) and is supported by the Rouen (France) biomedical informatics department of the University of Rouen (France). All the data are available in the Web Ontology language (OWL-2), an ontology-ready format fit for future use in the semantic web. The product, user guide and e-learning are available on http://3cgp.docpatient.net/ The Q-codes base is aimed at several uses: • Online repository of knowledge specific to GP/FM in several languages • Online PubMed linked bibliographic system easy to use for training in GP/FM • Resource for online e-learning • Resource for analysis of content of congresses in GP/FM in joint usage with ICPC-2 and indexation of gray literature • Research area : o automatic or semi-automatic congresses indexation system o linking GP/FM to the web of data and the linked data initiative The multilingual terminology in GP/FM Out of the online database we are proud to present you the • General Practice / Family Medicine Multilingual Terminology – English version • Terminologia Multilíngue de Clínica Geral / Medicina de Família e Comunidade - Versão em Português • Terminología multilingüe de la medicina general y de familia- Versión española • Terminologie multilingue de médecine générale et de famille – Version française • Een meertalige terminologie in de huisartsgeneeskunde - Nederlandse versie • Từ điển thuật ngữ đa ngôn ngữ về y học tổng quát và y học gia đình – (Vietnamese) • -Codes and definitions will be also available online in Korean and Turkish Those 6 books (60 pages) are readable / downloadable freely online on http://3cgp.docpatient.net/ and contain an explanation of the elaboration process of the taxonomy, a tabular list of the Q-Codes, the list of their definitions and an index. [less ▲]

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See detailGlomerular filtration rate in healthy living potential kidney donors: a meta-analysis.
Pottel, H; Hoste; DELANAYE, Pierre ULg

Poster (2016, November)

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See detailPreoptic glutamate and estradiol release during male sexual behavior
de Bournonville, Catherine ULg; de Bournonville, Marie-Pierre ULg; Aourz, Najat et al

Poster (2016, November)

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male ... [more ▼]

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male Japanese quail have also identified following exposure to a receptive female a rapid decrease in the activity of brain aromatase (AA) the enzyme responsible for the conversion of androgens into estrogens. These effects occur mainly within the medial preoptic nucleus (POM), a sexually dimorphic structure of the preoptic area that plays a key role in the activation of male sexual behavior and contains the highest AA in the brain. In vitro studies demonstrated that AA can be rapidly inhibited by calcium-dependent phosphorylations of the enzyme triggered by the activation of AMPA and kainate receptors. We confirmed here this rapid effect of glutamate on AA by injecting kainate in the POM of anesthetized males and measuring AA in the tissue after brain collection. AA in POM was inhibited in the kainate-injected hemisphere compared to the control hemisphere injected with vehicle. In a second experiment, we showed by in vivo microdialysis that glutamate is released in POM during copulation. These results thus suggest that glutamate controls dynamic changes of AA that occur in the brain during sexual interactions. To confirm that the decrease in AA leads to an actual reduction of local estradiol concentration, we quantified via microdialysis and radioimmunoassay changes in estradiol concentration in the male POM during sexual interactions with a female. Surprisingly, a dramatic elevation of estradiol was observed during copulation. Estradiol has been shown to enhance acutely male sexual motivation, therefore the function of its increase in the POM could be to maintain motivation during the entire sexual encounter. The decrease of AA observed ex vivo after copulation would then reflect a compensatory mechanism to restore baseline pre-copulatory conditions. Importantly, these results highlight that although long-term changes in AA are often used as a proxy for local estradiol concentrations, these two measures can show major short-term discrepancies possibly reflecting variations in estrogen turnover. [less ▲]

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See detailSpeech style manipulations in dysarthria in French-speaking children: Acoustic findings.
Ancelle, Josephine; Moya Gale, G.; Hetrick, B et al

Poster (2016, November)

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See detailRobustness of spatial autocorrelation tests
Ernst, Marie ULg

Poster (2016, November)

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis ... [more ▼]

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis over the classical one. Indeed, the analysis of spatial data is only meaningful if the spatial components bring information. Several measures of spatial autocorrelation are defined in the literature. Moran’s index, Geary’s ratio and Getis-Ord statistic are the most used statistics. Tests based on these measures have been developed in the literature using asymptotic and permutation results. They are used in practice in many fields, for instance in geography, economics, biogeosciences, medicine, ... However, these tests should be cautiously applied because they are not robust. A single contaminated observation can significantly modify their results. This poster is composed of two parts. Firstly, the robustness of already available tools for measuring spatial autocorrelation will be analysed. Secondly, alternative methods will be proposed to robustly test the spatial autocorrelation. [less ▲]

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See detailComparisons of estimated GFR and Cockcroft Gault equations: calibration against relative risk for all-cause mortality.
Warnock, DG; van den Brand, JAJG; DELANAYE, Pierre ULg et al

Poster (2016, November)

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See detailSilurian palynomorphs from the Precordillera basin, western Argentina: biostratigraphy and diversity trends
Garcia Muro, V.J.; Rubinstein, C.V.; Steemans, Philippe ULg

Poster (2016, October 27)

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See detailA benchmark for urban flood modelling
Arrault, Anaïs; Finaud-Guyot, Pascal; Archambeau, Pierre ULg et al

Poster (2016, October 25)

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See detailSpecific interests in Autism with versus without speech onset delay : the importance of perceptually versus thematically organized interests
Chiodo, Liliane ULg; Eusèbe, Sandrine; Majerus, Steve ULg et al

Poster (2016, October 25)

To explore whether autistic adults with versus without speech delay also differ in the perceptual vs thematic nature of their specific interests.

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See detailThermochemical conversion of sugar industry by-products to biofuel
Nicodème, Thibault ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Poster (2016, October 24)

Poster concernant la conversion des co-produits de l'industrie sucrière en biocarburants via la voie thermochimique. Description des procédés tels que la gazéification, Fischer-Trospch, la pyrolyse.

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See detailSurgical treatment of mucometra in an Alpine Goat.
Casalta, Hélène ULg; Knapp, Emilie ULg; Evrard, Laurence ULg et al

Poster (2016, October 21)

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See detailPhysiological Responses to training and competition in 1 to 4 star eventing horses
Kirsch, Katharina ULg; Düe, M.; Holzhausen, H. et al

Poster (2016, October 21)

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See detailReported post-operative analgesic administration to non-human primates: 2010 - 2015
Bertrand, Henri ULg; Flecknell, Paul; Brady, James et al

Poster (2016, October 21)

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See detailDevelopment of an analytical method for the simultaneous measurement of 10 biogenic amines in food
Douny, Caroline ULg; Benmedjadi, Soumaya; Blaszczyk, Marie et al

Poster (2016, October 21)

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See detailImpact of skimming and microfiltration processes on equol concentration in milk
Daems, Frédéric ULg; Ninane, Véronique; Romnee, Jean-Michel et al

Poster (2016, October 19)

Equol is a microbial metabolite of isoflavones that could be used as therapeutic agent against several diseases and cancers. Cow's milk could be a potential source of equol in the human diet, but more ... [more ▼]

Equol is a microbial metabolite of isoflavones that could be used as therapeutic agent against several diseases and cancers. Cow's milk could be a potential source of equol in the human diet, but more studies are needed on the changes in equol concentration during the technological processing of milk and dairy products. This first exploratory study sought to assess the impact of industrial skimming and microfiltration processes on equol concentration in conventional cow's milk. The milk samples used in this study came from bulk cow’s milk collected in Wallonia (Belgium), by a local dairy and processed in a local cheese factory. Six random sampling were conducted during spring and, for each of them, samples from raw, skimmed and microfiltered milk were analyzed. Equol was present in all samples at a concentration of between 3.2 and 10.3 µg.L-1. A Wilcoxon's signed rank test was then performed on the difference of data, having raw milk as reference, irrespective of the collection date. The results showed that the skimming process slightly increased the equol concentration in milk and therefore that equol had no or little affinity with the lipid milk fraction. The results also showed that, with the microfiltration process, a small proportion of equol was retained. Equol concentration has returned to the same level as that found in raw milk. This might be because of a chemical affinity either with bigger molecules that are physically retained or through direct interaction with the membrane. This scoping study paves the way for more extensive studies on the interaction between equol and other components of the milk. [less ▲]

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See detailA one year survey of seagrass primary productivity using the diving-PAM technique
Richir, Jonathan ULg; Abadie, Arnaud; Grosjean, Philippe et al

Poster (2016, October 18)

Marine magnoliophytes are major primary producers in coastal benthic habitats worldwide. They play a crucial role in the global carbon cycle (one of the more efficient blue carbon wells). Hence, it is ... [more ▼]

Marine magnoliophytes are major primary producers in coastal benthic habitats worldwide. They play a crucial role in the global carbon cycle (one of the more efficient blue carbon wells). Hence, it is necessary to characterise the eco-systemic services seagrass meadows provide. Posidonia oceanica (L.) Delile, the main Mediterranean seagrass species, has high foliar and belowground biomass production. Several methods have been used so far to measure its primary production (e.g., using incubation bells, optodes, biomass and elementary content measurements). A less used method relies on chlorophyll fluorescence measurements through the Pulse Amplitude Modulated (PAM) fluorometry method (Diving - PAM). In the framework of the STARECAPMED project, this study aimed to determine weekly to bimonthly over a one-year period the photosynthetic responses (Yield, relative Electron Transfer Rate, Rapid Light Curve) of P. oceanica. The survey was performed at 10m depth in a pristine meadow (Calvi, Corsica, France). To obtain reliable and comparable data, the protocol was standardized: measurements were performed on the convex middle part of the third leaf, at zenith, during shiny and calm weather days. Results showed that the plant displayed a well-marked seasonality. The mean ETR (μmol e- m-2 s-1 ) of the plant ranged from 2.17 in winter to 21.9 in summer and was linearly correlated throughout the year with the in situ irradiance (PAR irradiance taken perpendicularly to the surface, in the average leaf orientation). The ETR plateaus of the RLCs, ranging from 10.9 to 35.0, and their corresponding maximum PAR intensities evolved similarly. These results demonstrated both the adaptation and the seasonal plasticity of the meadow’s photosynthetic system. Overall the non-destructive PAM technique is a powerful and cost-effective tool to assess the primary productivity of seagrass meadows where other techniques (e.g. optodes) cannot be used and when direct sampling (e.g. biomass measurements) is not allowed. [less ▲]

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See detailMobility of 137Cs, 10Be and 210Pb in soil as a function of the soil texture, organic matter content and pH
de Tombeur, Félix ULg; Cornu, Sophie; Bourlès, Didier et al

Poster (2016, October 18)

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ... [more ▼]

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ecosystem services. In order to better constrain and quantify these processes, a kinetic quantification of the involved transfers based on vertical profiles of 210Pb (xs), 10Be and 137Cs has been developed for Luvisols in which soluble transfers could be neglected. The range of applicability of this type of approach was nevertheless not tested. In this work, we analyzed 210Pb (xs), 10Be and 137Cs in soils exhibiting gradients of organic matter, pH and < 2 μm fraction (Podzol, Andosol Ferralsol, Leptosol). Acidic soils and large organic matter contents were considered. The depth distribution of the studied isotopes was then interpreted as a function of the organic matter content, the soil pH and its < 2 μm fraction. This demonstrates that 10Be and 137Cs losses occur under soluble form in the Podzol, especially under forest, more acid, for the latter. Soluble 10Be transfers were also identified in the Andosol when they were considered as negligible in the Leptosol. For 210Pb (xs), soluble transfers are also probable for soils with pH less than 5.5 (Andosol and Ferralsol) and strong affinity for organic matter has been demonstrated. Thus, under acidic conditions and low < 2 μm fraction, soluble transfer cannot neglected anymore. [less ▲]

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See detailTowards precision beta-decay measurements with laser-cooled argon-35
Lenaers, Florence ULg; Glover, Rohan ULg; Bastin, Thierry ULg

Poster (2016, October 18)

Laser cooling and trapping techniques enjoy a solid reputation as powerful and elegant providers of well-localized, backing-free sources. Not only does this hold true within the atomic physics and quantum ... [more ▼]

Laser cooling and trapping techniques enjoy a solid reputation as powerful and elegant providers of well-localized, backing-free sources. Not only does this hold true within the atomic physics and quantum optics communities, but also beyond. Most notably, these techniques have recently joined the toolbox of low-energy, high-precision tests of the Standard Model. Our work with argon falls within the subset of nuclear beta decay studies, which have historically played a key role in establishing and testing our understanding of weak-interaction physics. In this framework, precision measurements of the beta asymmetry correlation parameter in the beta decay of Ar-35 have been identified as one promising probe for physics beyond the Standard Model. Our current work focuses on the development of a test magneto-optical trap (MOT) setup for Ar-40 as a preliminary step towards cooling and trapping Ar-35 atoms. In particular, much effort is being put into optimizing the efficiency of our source apparatus, which is crucial to achieving high enough statistics in the final trap. We present the current status of the experimental realization of our setup. Measuring the beta asymmetry correlation parameter requires highly spin-polarized samples. Therefore, in parallel to our experimental work, we are numerically investigating methods for generating, controlling and precisely measuring the degree of spin polarization of a sample of Ar-35 atoms. We also aim at being able to quickly switch between a polarized and a non-polarized sample so as to better understand systematic effects. We report on the progress of our theoretical modeling. [less ▲]

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See detailDiversity, dynamics and trophic ecology of animal communities associated to Posidonia oceanica (L.) Delile macrophytodetrital accumulation: synthesis of a ten year study
Lepoint, Gilles ULg; Borges, Alberto ULg; Champenois, Willy ULg et al

Poster (2016, October 17)

In the Mediterranean, Neptune grass Posidonia oceanica, produces a huge quantity of detrital biomass. These macrophytodetritus may accumulate in shallow waters, forming litter accumulations colonised by ... [more ▼]

In the Mediterranean, Neptune grass Posidonia oceanica, produces a huge quantity of detrital biomass. These macrophytodetritus may accumulate in shallow waters, forming litter accumulations colonised by abundant, yet understudied, animal communities. These accumulations are especially foraged by juvenile and adult fishes. Here, we aim to synthesize results obtained over the last ten years regarding diversity, dynamics and trophic ecology of associated meio- and macrofauna. Accumulations are found throughout the year but important seasonal and short-term variability in composition, quantity and physico-chemical parameters inside the accumulation is observed. Accumulations are dominated by respiration (litter degradation), however, primary production occurs at exposed surfaces (epiphytic production). Meio- and macrofauna have distinct traits in comparison to adjacent habitats (seagrass meadows or epilithic algae communities). A physico-chemical gradient occurs inside accumulations which partially defines assemblage composition and distribution. Meiofauna, in particular harpacticoid copepods, is diverse, abundant and composed of species from seagrass meadows, water column and sediment. In contrast, macrofaunal assemblages are simplified compared to the ones occurring in the seagrass meadows and are dominated by amphipods. Litter accumulations display a lower macrofaunal diversity than do seagrass meadows, but a higher abundance and animal biomass. Meio- and macrofauna show a high trophic diversity, dominated by ingestion and assimilation of epiphytes (macroalgae and, probably, detrivorous microbiota). Moreover, direct or indirect assimilation of carbon originating from seagrass detritus is demonstrated for many species. Although diverse trophic niches were observed, the assemblage showed a simplified trophic web structure compared to the seagrass meadows. Detritivorous organisms dominate this assemblage and are more abundant in the litter than in the living meadows. Consequently, according to its abundance and the fact it consumes directly and indirectly seagrass material, fauna associated to litter accumulation may play a significant role in the degradation and transfer to higher trophic level of detrital seagrass carbon. [less ▲]

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULg; Gridelet, Virginie ULg; Munaut, Carine ULg et al

Poster (2016, October 14)

Detailed reference viewed: 14 (3 ULg)
See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULg

Poster (2016, October 14)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells.: We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood.We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells [less ▲]

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See detailImpact of vitamin D binding protein on calculated free 25OH vitamin D
VRANKEN, Laura ULg; BECKAERT, Anne-Catherine; GENDEBIEN, Anne-Sophie ULg et al

Poster (2016, October 14)

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See detailAlkylcobalt as source of radicals for the controlled polymerization of less activated vinyl monomer
Demarteau, Jérémy ULg; Cordella, Daniela ULg; Kermagoret, Anthony et al

Poster (2016, October 13)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULg; Mendoza Gallego, Carlos ULg; Lismont, Marjorie ULg et al

Poster (2016, October 13)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 20 min while the same reaction in batch took 2h. [less ▲]

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See detailNovel promising way to synthesize non-isocyanate polyurethanes (NIPUs) for innovative coating applications against corrosion protection of metal surfaces
Panchireddy, Satyannarayana ULg; Grignard, Bruno ULg; Thomassin, Jean-Michel ULg et al

Poster (2016, October 13)

Polyurethanes (PUs) are one of the most widely used polymeric materials with applications in automotive, as sealants, adhesives, decorative, aircraft topcoats, for coatings or as foams for thermal and/or ... [more ▼]

Polyurethanes (PUs) are one of the most widely used polymeric materials with applications in automotive, as sealants, adhesives, decorative, aircraft topcoats, for coatings or as foams for thermal and/or acoustic insulation. Industrially, PUs are produced by copolymerization between polyols and polyisocyanates. Because of the toxicity issues related to the use of isocyanates, there is a need to develop greener and safer route to produce such polymers. This study reports on the synthesis of new sustainable isocyanates-free PU (NIPU) coatings for aluminium anticorrosion. In a first step, bio- and CO2-sourced cyclic carbonates monomers were synthesized by coupling of epoxydized vegetable oils with CO2 using a new efficient bicomponent organocatalyst. In second step formulations including cyclic carbonates/amines/additives were optimized to produce highly adhesive cross-linked NIPU coatings that show good resistance against solvents and long-term barriers preventing the corrosion of the aluminiumsubstrate [less ▲]

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See detailA Safe and Reliable Continuous-Flow System towards Methylphenidate Hydrochloride
Gerardy, Romaric ULg; Monbaliu, Jean-Christophe ULg

Poster (2016, October 13)

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous ... [more ▼]

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous-flow system implies the generation of an explosive organic azide, its consumption in a diazo transfer reaction, an intermediate purification step followed by a Rh(II)-catalyzed carbene C-H insertion reaction that produces threo-enriched N-Boc-methylphenidate. Boc-deprotection is carried out off-line and provides methylphenidate hydrochloride in 19% isolated yield (3 steps fully telescoped) or 38% isolated yield (sequential). [less ▲]

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See detailDesign and synthesis of Fe2O3 nanoparticles/N-doped porous carbon structures as high performance electrode for lithium ion battery
Alkarmo, Walid ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, October 13)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−S batteries, supercapacitors and fuel cells. In this context, a hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

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See detailSynthesis and characterization of non-isocyanate polyurethane (NIPU) hydrogels
Gennen, Sandro ULg; Grignard, Bruno ULg; Thomassin, Jean-Michel ULg et al

Poster (2016, October 13)

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as ... [more ▼]

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as hydrogels, it was used as wound dressing, soft contact lenses, drug delivery systems and scaffolds for tissue engineering. Classically, PU are synthesized by a step-growth polymerization between poly (di-)ols and poly (di-) isocyanates. Due to the toxicity of isocyanates, REACH regulations have changed and, today, there is a need to develop greener and safer route to produce isocyanate-free PUs. This contribution focus on the synthesis of NIPU hydrogels by copolymerizing a bifunctional CO2-sourced hydrophilic PEG bi-cyclic carbonate with diamines in presence of a cross- linker. The PEG bi-cyclic carbonates was prepared via a CO2/epoxide coupling reactions using a new efficient organocatalytic system based on the use of an ammonium salt (TBAI) in combination with a fluorinated alcohol. [less ▲]

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See detailPILs as a new active material for radical organic batteries
Aqil, Mohamed ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, October 13)

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See detailPhenotypic plasticity in a fossorial toad from a Pacific xeric forest (Ecuador)
Székely, Diana ULg; Szekely, Paul; Denoël, Mathieu ULg et al

Poster (2016, October 06)

For amphibians, optimal size at metamorphosis is determined by a trade-off between opportunities for growth and risks of mortality in aquatic and terrestrial habitats, and many species show a high degree ... [more ▼]

For amphibians, optimal size at metamorphosis is determined by a trade-off between opportunities for growth and risks of mortality in aquatic and terrestrial habitats, and many species show a high degree of plasticity in time and size at metamorphosis that allow them to adapt to unpredictable environments. We used as model a neotropical anuran species, the Pacific horned frog, Ceratophrys stolzmanni, that inhabits the Tumbesian dry forests of southwestern Ecuador, and lays its eggs in lentic habitats of variable size and unpredictable duration. We investigated (i) how tadpoles time their metamorphosis in order to escape a rapidly drying aquatic habitat, and (ii) the consequences of metamorphosis plasticity on the immediate and mid-term fitness of individuals. We conducted two experiments; in the first one we exposed 36 tadpoles to one of three water levels treatments: constant high, decreasing and constant low. Tadpoles that were raised in decreasing or low water level metamorphosed on average 13% and 10% faster than the ones from constant high water level, but at a lower body size and mass. Growth rates were among the highest recorded for tadpoles and individuals from the constant high water level treatment grew at a much faster rate than the others. In a second experiment, we sampled 72 freshly metamorphosed (Gosner stage 45) C. stolzmanni individuals of assorted sizes (SVL between 23.8 and 47.9 mm, body weight between 1.2 and 11.9 g). From this spectrum of sizes, we selected the 21 largest and 22 smallest individuals and evaluated the effects of body size at metamorphosis on trophic and locomotory performance parameters. We determined their growth rate and survival over the course of an activity season (i.e., two months). Our results show that bigger size at metamorphosis is correlated with better survival chances and performance. Larger individuals were more mobile, had bigger gape width and better survival rates (95% compared to 64% for small individuals). Growth rates were not correlated with initial size, and small individuals were capable of increasing their size at a higher rate. This indicates that, although size at metamorphosis has an immediate impact on fitness, smaller individuals are able to compensate with an increased growth rate. [less ▲]

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See detailAgeism among medical students
Corvol, Aline; Missotten, Pierre ULg; Gentric, Armelle et al

Poster (2016, October 06)

Introduction Negative stereotypes concerning elderly persons are widespread in general population. When they are adopted by health care professionals, it can decrease the quality of care or even induce ... [more ▼]

Introduction Negative stereotypes concerning elderly persons are widespread in general population. When they are adopted by health care professionals, it can decrease the quality of care or even induce disability. That’s why medical curriculum should take the question of ageism in consideration. Though, little is known about ageism among medical students and its determinants. Method We conducted a study on Rennes University (France) medical students (year 2 to year 6) using on line questionnaire. Validated tools, such as Fraboni Scale of Ageism, and innovating ones, such as verbal fluency task about aging and youth, have been proposed. Results have been compared with those obtained in Liège, Belgium, and analyzed according to age, gender, year of study, and geriatric training. Results Response rate was above 90%, leading to 814 replies. Replies, especially at the verbal fluency task, demonstrate the strength of negative stereotypes, with similar results in Rennes and Liège. No effect of courses or internship in geriatrics has been identified. Negative stereotypes seem lower in female students, and in those who have regular contact with elderly persons, especially if these contacts are good. Key conclusion Ageism is widespread in French medical students, whatever the year of study. Enhancing positive contacts between elderly persons and medical students may be an effective way to prevent it, but may be challenging to implement. Further studies could focus on implicit stereotypes to underplay the social desirability bias. [less ▲]

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See detailMise en place de pratiques agricoles de conservation: quel impact sur la vie du sol
Degrune, Florine ULg; Boeraeve, Fanny ULg; Vandenbol, Micheline ULg et al

Poster (2016, October 04)

Le projet Farm4Future a été lancé en 2015 et consiste en un suivi intégré d’un réseau d’exploitations agricoles belges en cours de transition vers des pratiques dites « de conservation ». Les ... [more ▼]

Le projet Farm4Future a été lancé en 2015 et consiste en un suivi intégré d’un réseau d’exploitations agricoles belges en cours de transition vers des pratiques dites « de conservation ». Les exploitations étudiées dans le cadre de ce projet sont caractérisées par des changements de gestion incluant notamment : (1) des associations de culture, (2) des couverts permanents, (3) une réduction du travail du sol et (4) l’introduction de structures vertes telles que les haies ou les bandes fleuries. Afin d’évaluer le potentiel agronomique et écologique de ces systèmes agricoles, une série de mesures ont été effectuées dans deux types de sol aux propriétés texturales contrastées : sable limoneux et un limon sableux. Afin d’obtenir un point de comparaison, les mêmes mesures ont été effectuées sur les parcelles des agriculteurs voisins restés en intensif et conventionnel. Parmi ces mesures, nous étudions la réponse de plusieurs indicateurs biologiques de qualité des sols telle que la diversité microbienne. L’étude a démontré un effet du type de pratiques agricoles sur la diversité microbienne en fonction du type de sol. Nous avons également identifié les microorganismes sensibles au type de pratiques agricoles. Cependant, l’étude nous amène à démontrer une grande hétérogénéité de réponses au sein de chacun de ces groupes. En effet, même si certains grands groupes sont globalement plus abondants sous pratiques de conservation (ou conventionnel), la réponse des microorganismes au sein de ces groupes à des niveaux taxonomiques plus fins (genre ou espèce) n’est pas uniforme. Aujourd’hui, même si les outils moléculaires de séquençage à haut débit ont permis de mettre en lumière l’incroyable diversité microbienne des sols, les services écosystémiques rendus par ces microorganismes sont encore très peu connus car moins de 1% des organismes est cultivable en laboratoire. [less ▲]

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See detailThe functional-biogeography of the reef fishes in the Gulf of California: over-importance of a handful of sites
Olivier, Damien ULg; Reyes Bonilla, Hector

Poster (2016, October 03)

Functional biogeography is a new discipline allowing to link biogeographical patterns of trait and species diversity. This new area of research has a role in conservation policy because it permits to ... [more ▼]

Functional biogeography is a new discipline allowing to link biogeographical patterns of trait and species diversity. This new area of research has a role in conservation policy because it permits to create functional maps to highlight hotspots of biodiversity. In this study, we evaluate the taxonomic and functional diversity of the reef-fishes in 30 “archipelagoes” widespread along the Gulf of California (GOC). The question before us is to highlight areas requiring a particular intention in conservation policy because they represent a large part of the legacy of reef-fish diversity in the GOC. To do so, we used a recently developed method to classify the reef-fishes species of the GOC in functional entities (FEs). The FEs are based on unique combinations of six categorical traits known to influence the functional role of fishes (e.g. size, diet…) and their number represent the level of functional diversity of the community. The results showed that the Baja California Sur has the most diversified reef-fishes community, both taxonomically and functionally (more than 90% of the species and of the functional diversity are represented). This result is mainly due to four archipelagoes that present a dazzling diversity, i.e. Carmen, San José, Espiritu Santo and Cerralvo. Each of these islands includes 60% or more of the taxonomic and functional diversity of the GOC. Two of these archipelagoes benefit of a protection level through the National Parks of Loreto and Espiritu Santo. However, the former is poorly functioning and the latter is under evaluation. The outcomes of the present study provide a global map of the reef-fishes diversity through the GOC, in term of species number and functional diversity. These results allow the identification of four major hotspots of diversity that should require a particular attention to preserve the legacy of reef-fishes diversity in the GOC. [less ▲]

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See detailThe peroxisome proliferator-activated receptor Alpha (PPARA) is expressed in non-somatotroph pituitary adenomas : a potential therapeutic target ?
Rotondi, S; Polidoro, MA; Morace, R et al

Poster (2016, October)

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See detailLRP4 mutations in bilateral renal agenesis with severe limb deficiencies
JACQUINET, Adeline ULg; LEE, Anna F.; SWENERTON, Anna et al

Poster (2016, October)

Introduction : Severe limb deficiencies have been reported in association with bilateral renal agenesis in several syndromes. Recently, truncating mutations in LRP4 have been associated with a lethal ... [more ▼]

Introduction : Severe limb deficiencies have been reported in association with bilateral renal agenesis in several syndromes. Recently, truncating mutations in LRP4 have been associated with a lethal autosomal recessive form of Cenani-Lenz syndrome (CLS). The objectives of our study was to delineate the phenotype of the third fetus, and through a search of syndromes, identify a group of disorders with overlapping phenotypes that may be allelic with CLS or due to mutations in the same pathway. Results : A male fetus at 22 weeks’ gestation was found to have bilateral renal agenesis by ultrasound. The pregnancy was interrupted and autopsy and skeletal survey revealed shortening of forearms and legs below the knees, radioulnar synostosis, foreshortened triangular shaped tibia and fibula, and oligosyndactyly with disorganized metacarpals, metatarsals and phalanges. Bilateral renal agenesis, absent ureters and small bladder were confirmed. Additional features included downslanting palpebral fissures, high-arched palate, small mouth, low-set and posteriorly rotated ears, nuchal oedema, pulmonary hypoplasia, hypospadias and absent olfactory nerves. An autosomal recessive disorder was suspected, given a prior obstetric history of termination at 18 weeks for unilateral limb anomalies and cleft lip. Based on the aforementioned renal and limb anomalies, the lethal form of CLS was considered. Sequencing of LRP4 identified a paternally inherited variant (c.919+3G del) and a maternally inherited variant (c.4808T>A ;p.Ile1603Val). Both variants were rare in the general population and bioinformatically predicted to be damaging. We compared the phenotype with the two previously reported fetuses as well as with syndromes and disorders that may present prenatally with genitourinary anomalies plus acromesomelia of upper and lower limbs. We found significant overlap with a subset of cases of acro-renal-uterine-mandibular syndrome presenting with high-arched palate plus or minus micrognathia and microglossia, severe limb anomalies affecting the middle and distal segments, oligosyndactyly, renal agenesis, uterine anomalies and absence of olfactory nerves. Conclusion : Based on the clinical features seen in three lethal cases of CLS, we propose that acro-renal-uterine-mandibular syndrome may be allelic with CLS or due to mutations in the molecular pathway involving LRP4. [less ▲]

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See detailADAM28 - Generation of a conditional ADAM28 knock-out mouse Implication of ADAM28 in metastasis dissemination
Gérard, Catherine ULg; Rocks, Natacha ULg; Carnet, Oriane ULg et al

Poster (2016, October)

Introduction: ADAMs (a disintegrin and metalloproteinase) and ADAMTS proteinases (a disintegrin and metalloproteinase with thrombospondin motifs) are MMP (matrix metalloproteinase) -related enzymes ... [more ▼]

Introduction: ADAMs (a disintegrin and metalloproteinase) and ADAMTS proteinases (a disintegrin and metalloproteinase with thrombospondin motifs) are MMP (matrix metalloproteinase) -related enzymes, bearing a multi-domain structure. ADAM28 is a multipotent membrane-bound proteinase and its expression in tissues derived from the foregut in embryonic tissues suggests its involvement in the organogenesis of the respiratory tract. Moreover, ADAM28 is highly overexpressed in non-small-cell lung cancer samples. Notably through a global proteomic approach, we recently identified an upregulation of ADAM28 after induction of lung inflammation in a mouse model of asthma. In addition to data published in the literature and to our findings about ADAM28 expression in various pathological tissues, intrinsic characteristics of this proteinase argue for considering it as a potential regulator of cellular signalling pathways leading to an inflammatory pulmonary microenvironment and, eventually, to carcinogenesis. Indeed, ADAM28 bears an active catalytic domain and interacts in a non-proteolytic manner with some integrins (α4β1) and some P-selectin ligands involved in inflammatory cell migration. ADAM28 was also reported to display the capacity to cleave key mediators such as Von Willebrand factor, IGFBP3 (Insulin-like growth factor-binding protein 3) and CTGF (Connective Tissue Growth Factor). Very recently, ADAM28 was shown to shed pro-TNF-alpha suggesting an important role in tumour control. The present project aims at characterizing molecular mechanisms leading to tumour development in lungs. In the first part of this work, we intend to characterize the effects of ADAM28 depletion on physiological and pathological processes such as tumor development and metastatic dissemination in ADAM28 conditional knock-out mice. Methods: To understand the implication of ADAM28 in lung tumour development we instillated lungs of mice displaying a full knock-out genotype for ADAM28 with Lewis Lung Carcinoma cells and B16K1 melanoma cells. ADAM28 KO mice were also injected intravenously and a subcutaneously with these tumour cells. Results: There is no spontaneous phenotype for ADAM28 full knock-out animals. Preliminary results show an impaired lung tumors engrafment in ADAM28 wild type animals when compared to heterozygous or knock out littermates. Conclusion: This unique mouse strain provides a very precious tool to further investigate ADAM28 implication in various disease models including tumour growth and dissemination. The role of ADAM28 as a pro-tumor factor is widely described in the literature whereas our results suggest that ADAM28 has an anti-tumor function. [less ▲]

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See detailInvestigating the potential of Cymodocea nodosa (Ucria) Ascherson as a coastal carbon sink coupling marine habitat cartographies and in situ nondestructive sampling
Abadie, Arnaud ULg; Richir, Jonathan ULg; Pieraccini, Riccardo ULg et al

Poster (2016, October)

Seagrass meadows are major carbon sinks, trapping about 10% of the total CO2 sequestrated in the oceans. In the Mediterranean, a major focus has been made on the climax species Posidonia oceanica (L ... [more ▼]

Seagrass meadows are major carbon sinks, trapping about 10% of the total CO2 sequestrated in the oceans. In the Mediterranean, a major focus has been made on the climax species Posidonia oceanica (L.) Delile, while other species remained little studied. In the framework of the STARECAPMED project, we thus chose to study Cymodocea nodosa (Ucria) Ascherson, a pioneer species with a rapid turnover and an expected high stocking capacity. Furthermore, the area covered by that species has been largely underestimated. In order to fill these two knowledge gaps, we first mapped all seagrass habitats within a Mediterranean bay (Calvi, Corsica, France) using side scan images, aerial photographs and ground truths. This cartography was followed by seasonal in situ density measurements and non-destructive shoot sampling (leaf cutting). Samplings were performed at different depths (5 to 23 m depth) in 6 contrasted stations (small patchy meadows to continuous beds) in order to cover all the existing facies of the bay. Elementary contents (carbon, nitrogen and stable isotope ratios) were measured in laboratory. This first work shows that C. nodosa meadows in Calvi Bay cover an area of 0.498 km2. Carbon stocks of the leaves reached 0.25 tons in winter and 2.72 tons in summer. Their nitrogen contents showed a marked seasonality with a maximum value of 0.020 mgN.m-2 in July and a minimum value of 0.005 mgN.m-2 in March. Some modifications in the trophic conditions of the water column at several stations were put in an obvious through the N stable isotopes values, mostly during the summer period. The actual underestimation of the area covered by that species in Calvi Bay has been properly mapped thanks to side scan sonar techniques revealing, together with elementary content analysis, its importance in the carbon balance of coastal areas. [less ▲]

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See detailA membrane-type- matrix metalloproteinase (MT1-MMP) - discoidin domain receptor 1 axis regulates collagen-induced apoptosis in breast cancer cells
Assent, Delphine; Bourgot, Isabelle ULg; Hennuy, Benoit et al

Poster (2016, October)

During tumour dissemination, invading breast carcinoma cells become confronted with a reactive stroma, a type I collagen-rich environment endowed with anti-proliferative and proapoptotic properties. To ... [more ▼]

During tumour dissemination, invading breast carcinoma cells become confronted with a reactive stroma, a type I collagen-rich environment endowed with anti-proliferative and proapoptotic properties. To develop metastatic capabilities, tumour cells must acquire the capacity to cope with this novel microenvironment. How cells interact with and respond to their microenvironment during cancer dissemination remains poorly understood. To address the impact of type I collagen on the fate of tumour cells, human breast carcinoma MCF-7 cells were cultured within three-dimensional type I collagen gels (3D COL1). Using this experimental model, we demonstrate that membrane type-1 matrix metalloproteinase (MT1-MMP), a proteinase overexpressed in many aggressive tumours, promotes tumour progression by circumventing the collagen-induced up-regulation of BIK, a pro-apoptotic tumour suppressor, and hence apoptosis. A transcriptomic analysis was performed to decipher the molecular mechanisms regulating 3D COL1-induced apoptosis in human breast cancer cells. Control and MT1-MMP expressing MCF-7 cells were cultured on two-dimensional plastic plates or within 3D COL1 and a global transcriptional time-course analysis was performed. Shifting the cells from plastic plates to 3D COL1 activated a complex reprogramming of genes implicated in various biological processes. Bioinformatic analysis revealed a 3D COL1-mediated alteration of key cellular functions including apoptosis, cell proliferation, RNA processing and cytoskeleton remodelling. By using a panel of pharmacological inhibitors, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase specifically activated by collagen, as the initiator of 3D COL1-induced apoptosis. Our data support the concept that MT1-MMP contributes to the inactivation of the DDR1-BIK signalling axis through the cleavage of collagen fibres and/or the alteration of DDR1 receptor signalling unit, without triggering drastic alterations of the transcriptome of MCF-7 cells. [less ▲]

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See detailRegulation of breast carcinoma cell proliferation and apoptosis by type I collagen aging and 3D confinement involves differential activation of DDR1
Saby, Charles; Rammal, Hassan; Magnien, Kevin et al

Poster (2016, October)

During breast carcinoma development, cells are confronted to a type I collagen-rich environment which induces anti-proliferative and pro-apoptotic effects (Maquoi et al., Oncogene, 2012). These effects ... [more ▼]

During breast carcinoma development, cells are confronted to a type I collagen-rich environment which induces anti-proliferative and pro-apoptotic effects (Maquoi et al., Oncogene, 2012). These effects occurred only in 3D cell matrix and involved Discoidin Domain Receptor 1 (DDR1) but not β1 integrin (Assent et al., PlosOne, 2015). During aging, type I collagen undergoes post-translational modifications which have a detrimental effect on type I collagen fibrillar organization (Aït-Belkacem, J Biomed Optics, 2012 ; Guilbert et al., Oncotarget, 2016). This property is well known to be requested to activate DDR1. Here, we address the effect of type I collagen aging on cell proliferation inhibition and apoptosis induction in breast carcinoma cells in 3D cell confinement system. Type I collagen was extracted from tail tendons of 2 months (adult) and 2 years (old) rats, and MCF-7 and ZR-75-1 cells were used as an experimental model. We provide evidence for a decrease in cell proliferation by adult collagen but not by old one. This effect involves a higher phosphorylation of DDR1 by adult collagen when compared to the old one. Inhibition of DDR1 kinase activity by the specific inhibitor DDR1-IN-1 induced an increase in cell proliferation to a level similar to that observed in old collagen. An increase in cell proliferation was also observed using siRNA targeting DDR1 mRNA. At the opposite of old collagen, adult collagen was also able to trigger apoptosis by inducing an increase in expression of the pro-apoptotic protein (BIK). Inhibition of DDR1 kinase activity induced a decrease in BIK expression and apoptosis. Our data support the concept that aging contributes to the loss of the growth suppression and apoptotic effect of collagen on breast carcinoma, but only in 3D cell confinement model, by inactivating the DDR1-BIK signaling axis via a downregulation of DDR1 activation. [less ▲]

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See detailRobust discriminant analysis based on the joint graphcial lasso estimator
Aerts, Stéphanie ULg; Croux, Christophe; Wilms, Ines

Poster (2016, October)

Linear and Quadratic Discriminant Analysis (LDA/QDA) are the most often applied classification rules under the normality assumption. When there is not enough data, the quadratic rule, which requires the ... [more ▼]

Linear and Quadratic Discriminant Analysis (LDA/QDA) are the most often applied classification rules under the normality assumption. When there is not enough data, the quadratic rule, which requires the estimation of one precision matrix in each class, is often replaced by the linear one, based on the homoscedasticity assumption. This strong assumption is however rarely verified in practice and ignores the intrinsic différences between groups that may be of particular interest in the classification context. In this aper, alternatives to the usual maximum likelihood estimates for the precision matrices are proposed that borrow strength across classes while allowing for heterogeneity at the same time. This results in a classifier that is intermediate between QDA and LDA. Moreover, our estimator is sparse: the undesirable effect of uninformative variables is reduced. The performance of the method is illustrated through simulated and real dataset examples. [less ▲]

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See detailMultimodal Connectivity-Based Parcellation of the Brain
Reuter, Niels; Plachti, Anna; Genon, Sarah ULg et al

Poster (2016, October)

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See detailStudy of the SV2A protein role in Epilepsy.
Bartholomé, Odile ULg; Van Den Ackerveken, Priscilla ULg; Wislet, Sabine ULg et al

Poster (2016, October)

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See detailSystème d’exploitation et potentialités économiques des cuvettes oasiennes du sud-est du Niger
Malam Boukar, Awa Krou; Yamba, Boubacar; Lebailly, Philippe ULg

Poster (2016, September 28)

Les cuvettes oasiennes du sud-est nigérien sont sources de production alimentaire (JAHIEL. 1998). C’est pourquoi, les populations locales sont dépendantes des cuvettes pour leurs productions alimentaires ... [more ▼]

Les cuvettes oasiennes du sud-est nigérien sont sources de production alimentaire (JAHIEL. 1998). C’est pourquoi, les populations locales sont dépendantes des cuvettes pour leurs productions alimentaires et leurs revenus (TYCHON B. et al. 2009). Cette étude vise à démontrer l’importance socio-économique des cuvettes oasiennes du sud-est nigérien. [less ▲]

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See detailClimatic and management drivers of CO2 exchanges by a production crop: Analysis over three successive 4-year crop rotation cycles
Buysse, Pauline ULg; Manise, Tanguy ULg; De Ligne, Anne ULg et al

Poster (2016, September 27)

Carbon dioxide (CO2) exchanges between crops and the atmosphere are influenced by both climatic and crop management drivers. The investigated crop, situated at the Lonzée Terrestrial Observatory (LTO ... [more ▼]

Carbon dioxide (CO2) exchanges between crops and the atmosphere are influenced by both climatic and crop management drivers. The investigated crop, situated at the Lonzée Terrestrial Observatory (LTO, candidate ICOS site) in Belgium and managed for more than 70 years using conventional farming practices, was monitored over three complete sugar beet/winter wheat/potato/winter wheat rotation cycles from 2004 to 2016. Continuous eddy-covariance measurements and regular biomass samplings were performed in order to obtain the daily and seasonal Net Ecosystem Exchange (NEE), Gross Primary Productivity (GPP), Total Ecosystem Respiration (TER), Net Primary Productivity (NPP), and Net Biome Production (NBP). Meteorological data and crop management practices were also recorded. Over the 12 years, NEE was negative (-4.34 kg C m-2) but NBP was positive (1.05 kg C m-2), i.e. as soon as carbon exportation by harvest and carbon importation (manure, slimes) are included in the budget, the site behaves as a carbon source. At the crop rotation scale (4 years) it was quite remarkable to observe that NBP was very similar over the three rotations (0.30-0.36 kg C m-2), despite climatic and management differences between years. Crop type impacted carbon exchanges, with sugar beet and winter wheat crops leading to higher net carbon sequestration than seed potato crops. For one given crop, larger growth length and cumulated global radiation drove larger cumulated NEE. Net carbon emissions were observed during intercrops, but growing mustard during these periods reduced their rates and provided carbon residues to the soil. NBP values suggest that one sixth of the total soil organic carbon stock at LTO (6.23 ± 0.16 kg C m-2 in [0, 60] cm) would be lost in 12 years. Large uncertainties (mostly due to biomass measurements) affect NBP estimates, but still, this figure is huge and should encourage cultural practices returning carbon to the soil. [less ▲]

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See detailDEVELOPMENT OF NOVEL CATIONIC AND LIGAND-GRAFTED ANIONIC LIPOSOMES FOR BRAIN-TARGETED DRUG DELIVERY
Karim, Reatul ULg; Palazzo, Claudio ULg; Laloy, Julie et al

Poster (2016, September 27)

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See detailInfluence of groundwater exchanges on the efficiency of Underground Pumped Storage Hydroelectricity plants using open pit mines
Pujades, Estanislao ULg; Bodeux, Sarah ULg; Orban, Philippe ULg et al

Poster (2016, September 27)

Underground Pumped Storage Hydropower (UPSH) plants can be used to manage the production of electrical energy according to the demand. These plants consist in two reservoirs, the upper one is located at ... [more ▼]

Underground Pumped Storage Hydropower (UPSH) plants can be used to manage the production of electrical energy according to the demand. These plants consist in two reservoirs, the upper one is located at the surface while the lower one is underground. The energy is stored by pumping water from the lower to the upper reservoir and produced by releasing water from the upper to the lower one. As a result, the hydraulic head in the lower reservoir varies continuously. UPSH plants interact with the surrounding aquifers exchanging groundwater. These groundwater exchanges, which play an important role in the evolution of the hydraulic head inside the underground reservoir, are a fact of concern in the selection of pumps and turbines because their efficiency varies with respect to the head difference between the two reservoirs. Therefore, the aquifer parameters should be considered in the selection of pumps and turbines. In this context, with an UPSH plant made up by an open pit mine, we study numerically (1) the influence of groundwater exchanges on the efficiency and (2) how the hydraulic head evolution varies depending on the aquifer properties. The relation among the groundwater exchanges, the efficiency of pumps and turbines and the aquifer parameters is considered by comparing the numerical results of several simulations. It is shown that groundwater exchanges are not negligible when optimizing the efficiency of UPSH plants. A priori, low hydraulic conductivity geological media were preferred to decrease interactions with the open-pit or the cavity used as lower reservoir. Taking into account the pump/turbine performance curves, it appears that, on the contrary, the global efficiency would be increased if the surrounding medium facilitates large groundwater exchanges because hydraulic head variations are softened. [less ▲]

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See detailMUCOADHESIVE SPONGES WITH PEGYLATED LIPOPLEXES: TOWARDS A SUSTAINED VAGINAL DELIVERY OF siRNA
Furst, Tania ULg; Dakwar, Georges; zagato, Elisa et al

Poster (2016, September 26)

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See detailSmall molecule ligands for the orphan GPR27
Dupuis, Nadine ULg; Franssen, Delphine ULg; Laschet, Céline ULg et al

Poster (2016, September 26)

Background G protein-coupled receptors (GPCRs) are involved in many physiological processes and constitute the target of around 30% of marketed therapies. Nonetheless, ~100 human GPCRs have no known ... [more ▼]

Background G protein-coupled receptors (GPCRs) are involved in many physiological processes and constitute the target of around 30% of marketed therapies. Nonetheless, ~100 human GPCRs have no known ligand and are designated as "orphan". This project focuses on GPR27, a rhodopsin-like alpha orphan of the SREB family (Super conserved Receptors Expressed in the Brain), presumably involved in the regulation of insulin secretion [1]. Methods In order to identify small molecules activating GPR27, we developed a firefly luciferase complementation assay (based on [2]) to assess the binding of ß-arrestin2 to the activated GPCR. To increase the affinity for and strengthen the interaction with ß-arrestin2, a GPR27-V2R chimera has been used for library screening. Results Small molecules activating GPR27-V2 have been identified in the DiverSetTM library (ChemBridge). After exclusion of non-specific activities using another unrelated GPCR, two compounds sharing a common scaffold with activity in the low micromolar range were selected for further investigations. We confirmed their agonist profile by performing complete concentration-response curves on our arrestin complementation assay as well as orthogonal assays. These compounds show good specificity being inactive on GPR85-V2 and GPR173-V2 (the two other SREB members). With these original tools, we characterized the recruitment of ß-arrestin2 to activated GPR27 WT. Conclusion We identified small molecule ligands for GPR27 that will serve as valuable tools for studying the pharmacology of GPR27 as well as its physiological roles, for example in insulin secretion. 1 Ku G.M., Pappalardo Z., Luo C.C., German M.S., McManus M.T. An siRNA Screen in Pancreatic Beta Cells Reveals a Role for Gpr27 in Insulin Production. PLoS genetics. 2012, 8, e1002449. 2 Takakura H., Hattori M., Takeuchi M., Ozawa T. Visualization and Quantitative Analysis of G Protein-Coupled Receptor−β-Arrestin Interaction in Single Cells and Specific Organs of Living Mice Using Split Luciferase Complementation. ACS Chem. Biol. 2012, 7, 901−910. [less ▲]

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See detailQuelles réponses face à la fatigue vocale? Variabilité inter-individuelle des modifications acoustiques de la voix au cours de la journée
Remacle, Angélique ULg; Gerber, Silvain; Garnier, Maëva

Poster (2016, September 26)

Objectifs: 1) décrire l’évolution de 4 indicateurs acoustiques de la fatigue vocale au cours d’une journée de travail et examiner leur universalité dans l'échantillon, 2) déterminer si différentes ... [more ▼]

Objectifs: 1) décrire l’évolution de 4 indicateurs acoustiques de la fatigue vocale au cours d’une journée de travail et examiner leur universalité dans l'échantillon, 2) déterminer si différentes typologies de réponse à la fatigue vocale peuvent être distinguées. Population: 22 enseignantes enregistrées le matin et le soir après leur journée de travail, à 3 temps de l’année: octobre, décembre, et février. Tâche: voyelle [a] tenue produite 5 fois à intensité et fréquence confortables. Paramètres mesurés avec Praat et moyennés sur les 5 répétitions: F0 (Hz), rapport harmoniques/bruit (HNR, dB), local Jitter (%), et local Shimmer (%). Les variations des moyennes entre le soir et le matin (Δ) sont calculées pour chaque paramètre. Résultats: En accord avec la littérature, le Δ moyen pour les 22 participantes aux 3 temps montre une augmentation de F0 et HNR, et une diminution de Jitter et Shimmer au cours de la journée. Cependant, ces résultats ne sont pas généralisés dans notre échantillon. Une analyse hiérarchique ascendante permet d'identifier 3 typologies de réponse à la fatigue vocale, observées suite à la journée de travail. Typologie 1: tendance inverse à la littérature (ΔF0 et ΔHNR négatifs, Δjitter et Δshimmer positifs). Comportement hypofonctionnel en réponse à la fatigue vocale. Typologie 2: tendance et amplitude similaires à la littérature (ΔF0 et ΔHNR positifs, Δjitter et Δshimmer négatifs). Réponse attendue, adaptation saine à la fatigue vocale. Typologie 3: tendance similaire à la littérature mais d’amplitude très marquée. Comportement hyperfonctionnel, suspicion d’un trouble vocal. [less ▲]

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See detailGlucose production: influence of the datasets choice on LCA results
Gerbinet, Saïcha ULg; Belboom, Sandra ULg; Léonard, Angélique ULg

Poster (2016, September 22)

The aim of this study is to have a good understanding of the environmental impact of glucose production. Glucose is generally produced from corn or wheat. Since agricultural processes are known to be ... [more ▼]

The aim of this study is to have a good understanding of the environmental impact of glucose production. Glucose is generally produced from corn or wheat. Since agricultural processes are known to be difficult to evaluate by LCA, the results obtained with two different LCA databases, Gabi and EcoInvent, are compared in this work. The production of glucose from raw materials can be divided in two steps: the agricultural step allowing the plant production, and the conversion step including the extraction of the starch from the plant and its hydrolysis into glucose. Preliminary results underline the high impact of the agricultural step, so a special attention has been paid to these data. Specific Belgian data collected by the Walloon Agricultural Research Centre (CRA-W) (2014) [1] have been used as primary data (yield, amount of fertilizers, etc.), either using EcoInvent or Gabi datasets background data to model fertilizers, diesel consumption, etc. A third model was built using only data available in Ecoinvent for corn and wheat cultures. For the conversion step, literature data have been used along with some industrial data. As few studies are available in the literature concerning starch hydrolysis, the focus has been placed on data validation (mass balance checks, cross-reference information, etc.). Based on these multiple sources, it is possible to compare the LCA results for the production of 1 kg of glucose for three different cases, summarized in the following table. Table 1: Summary of modelled cases Agricultural step Conversion steps Primary data Dataset Primary data Dataset Case 1 Belgian GaBi Literature + Industry GaBi Case 2 Belgian Ecoinvent Literature + Industry Ecoinvent Case 2 Ecoinvent Literature + Industry Ecoinvent The results obtained using these three models will be presented, at both the inventory and impact assessment steps. They show significant differences and highlight the need to understand in depth the involved assumptions when developing the datasets, in addition to the ones adopted for the inventory. [less ▲]

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See detailValorization of Seeds from Some Field Border Flowering Seeds
Paul, Aman ULg; Danthine, Sabine ULg; Mutwale Kapepula, Paulin ULg et al

Poster (2016, September 20)

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It ... [more ▼]

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It could be really interesting to valorize some commonly grown plant in these strips to render food or health promoting compounds. With this objective in mind the seeds of Achillea millefolium, Anthriscus sylvestris and Prunella vulgaris were investigated for lipids, proteins and phenolic content. Further the lipids were analyzed for fatty acid profile using gas chromatography and the phenolic compounds in the methanolic extract of defatted seeds were identified using HPLC-DAD. The antiradical activity of the methanolic extracts obtained from defatted seeds was investigated using DPPH and ABTS assays. The anti-inflammatory potential of these seed extracts was evaluated on the reactive oxygen species (ROS) production by stimulated neutrophils and on the specific activity of myeloperoxidase (MPO), a pro-oxidant enzyme marker of inflammation. Seeds from all three plants were analyzed with interesting levels of lipids, proteins and phenolic content. Linoleic acid, oleic acid and alpha-linolenic acid were the major fatty acids analyzed in A. millefolium, A. sylvestris and P. vulgaris respectively. On the other hand different phenolic acid formed the major phenolic constituents. Seed extracts displayed high ABTS and DPPH radical-scavenging activities in a dose dependent manner. Also a strong dose dependent anti-inflammatory activity of all three extracts was observed against ROS production by neutrophils and MPO activity. Results indicate that these seed show a great potential to render lipids which could be utilized as human food, further the defatted seeds could be directly included in human diet due to interesting levels of proteins and anti-inflammation ability. [less ▲]

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See detailImpact du nombre d’alternatives lors d’une tâche de reconnaissance à choix-forcé sur les processus de reconnaissance dans le vieillissement normal
Simon, Jessica ULg; Gilsoul, Jessica ULg; Bastin, Christine ULg

Poster (2016, September 19)

Les tâches de reconnaissance à choix forcé sont classiquement utilisées pour évaluer la mémoire de reconnaissance. Cependant, aucune étude n’a spécifiquement investigué l’impact du nombre d’alternatives ... [more ▼]

Les tâches de reconnaissance à choix forcé sont classiquement utilisées pour évaluer la mémoire de reconnaissance. Cependant, aucune étude n’a spécifiquement investigué l’impact du nombre d’alternatives sur les performances mnésiques des participants âgés. Nous voulons déterminer, d’une part, si le nombre d’alternatives proposées - deux ou trois – et d’autre part, si le degré de similarité entre la cible et ses leurres ont un impact sur les stratégies de récupération mises en place au cours de la tâche. Pour ce faire, nous avons recruté 20 participants jeunes et 20 participants âgés. Lors de la tâche de reconnaissance, nous leur avons demandé de choisir, parmi deux ou trois photographies de visages, celui qui a été présenté précédemment. Certains couples cibles-leurres étaient plus similaires que d’autres (partage de 60% de caractéristiques communes, contre 40%). Pour chaque item sélectionné, les participants devaient expliquer ce qui a guidé leur choix. Les premières analyses (ANOVA à mesures répétées 2 (groupes) x 2 (alternatives) x 2 (similarité) sur les deux dernières mesures, p<0,05) montrent que les performances entre les groupes sont équivalentes pour la tâche de reconnaissance à deux alternatives. A l’inverse, nous avons observé que les participants jeunes avaient significativement de meilleures performances que les âgés dans la tâche de reconnaissance à trois alternatives. Les profils mnésiques des participants seront étudiés à la lumière des modèles à deux processus de la reconnaissance. Nous faisons l’hypothèse d’un recours plus fréquent à la recollection chez les jeunes dans la tâche de reconnaissance à trois alternatives (vs deux alternatives), alors que le choix de l’item se ferait chez les âgés, en comparant directement les degrés de familiarité associés à chaque item et en sélectionnant le plus familier (Norman & O’Reilly, 2003). [less ▲]

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See detailMetabolites from media supplemented with 3’-sialyllactose and fermented by bifidobacteria have an antivirulent effect against intestinal pathogens
Bondue, Pauline ULg

Poster (2016, September 16)

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides ... [more ▼]

Introduction Complex oligosaccharides from human milk (HMO) promote growth of bifidobacteria such as Bifidobacterium bifidum [1]. Whey, a by-product of dairy-industry, contents complex oligosaccharides (BMO) similar to HMO, which are mainly represented in colostrum by 3’-sialyllactose (3’SL) [2]. Bifidobacterium crudilactis, a species of bovine origin, encodes for β galactosidases and α-glucosidases and could therefore be able to metabolise those BMO [3; 4; 5]. In addition, fermentation products from bifidobacteria can produce antivirulent activity against intestinal pathogenic bacteria [6; 7]. This study focused on capacity of bifidobacteria to metabolise BMO, more particularly 3’SL, and on potential antivirulent effect of cell-free spent media (CFSM) against virulence gene expression of pathogenic bacteria. Material and methods B. bifidum BBA1 and B. crudilactis FR/62/B/3 isolated respectively from breastfed children feces and from cow raw milk cheese were grown on media supplemented with BMO or 3’SL, as sole source of carbon. The CFSM were harvested after centrifugation of cells culture, freeze-dried and concentrated 10 fold. Next, their effects were tested against virulence gene expression using ler and hilA promoter activity of luminescent constructs of Escherichia coli 0157:H7 ATCC 43888 and Salmonella Typhimurium SA 941256, respectively. The effect was confirmed on wild type strains of E. coli O157:H7 ATCC 43890 and S. Typhimurium ATCC 14028 using RT-qPCR. Results Both strains were able to grow in presence of whey or 3’SL, but B. crudilactis showed the best growth compared to B. bifidum. The highest cell concentrations were observed with media containing whey (8.9 ± 0.6 log cfu/ml and 8.1 ± 0.3 log cfu/ml, respectively). CFSM from fermented media supplemented with 3’SL resulted in under-expression of hilA and ler genes for the luminescent constructs and in under-expression of ler (ratios of -15.4 and -8.1) and qseA (ratios of -2.1 and -3.1) genes for the wild type strain of E. coli O157:H7. No effect was observed for the wild type strain of S. Typhimurium. Discussion B. crudilactis presented the best growth potential probably because its genome encodes the enzymatic machinery to use BMO (β galactosidases and α-glucosidases) [3; 4; 5]. The positive effect of media supplemented with milk products on growth of probiotics has been demonstrated previously [8]. CFSM obtained from media supplemented with 3’SL down-regulate several virulence genes of E. coli O157:H7 and potentially S. Typhimurium. This effect has been observed previously with CFSM obtained from fermentation of lactic acid bacteria or bifidobacteria, by production of antivirulent metabolites [2; 3]. BMO combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the exact nature of the active molecules. References 1. Garrido D. et al. (2013). Microbiology 159: 649-664. 2. Urashima T. et al. (2013). Biosci Biotechnol Biochem 77: 455-466. 3. Sela D. A. (2011). Int J Food Microbiol 149: 58-64. 4. Milani C. et al. (2014). Appl Environ Microbiol 80: 6290-6302. 5. Bondue P. & Delcenserie V. (2015). Korean J Food Sci Anim Resour 35: 1-9. 6. Medellin-Pena M. J. et al. (2007). Appl Environ Microbiol 73: 4259-4267. 7. Bayoumi M. A. & Griffiths M. W. (2012). Int J Food Microbiol 156: 255-263. 8. Champagne C. P. et al. (2014). Can J Microbiol 60: 287-295. [less ▲]

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See detailOn-farm Evolution of Genetic Diversity of Four Old Varieties of Phaseolus vulgaris L.
Caproni, Leonardo; Raggi, Lorenzo; Klaedtke, Stephanie ULg et al

Poster (2016, September 16)

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which ... [more ▼]

On-farm evolution of crop genetic diversity has a key role in adaptation of agricultural systems to changes. The aim of this work was to understand how four different old common bean varieties, which being made of different genotypes can be referred to as populations, evolved on-farm during three years of multiplication (from 2012 to 2014) in two different experimental sites (Brittany and Luxembourg) under organic farming conditions. The four populations (Flageolet Chevrier Vert, Rognon de Coq, Roi des Belges, Saint Esprit à Œil Rouge) were chosen among those grown by two small-scale organic seed farmers while the commercial variety (Calima) was included as control. In 2015 the initial and the evolved populations were grown in a common field and characterized with morpho-phenological traits. In the same year, young leaf tissues were collected from 94 samples per population (total of 470 samples) for DNA extraction and molecular characterization. Genotyping was carried out using 22 SSR loci and fluorescent PCR amplicons analysed on an ABI3130xl sequencer. Molecular data were then used for multivariate analysis, analysis of molecular variance and pair-wise Fst statistics calculation. Genotyping resulted in the production of about 20K data points. A total of 83 different alleles were identified in 16 polymorphic loci ranging from two (BMb293, BM156, BMd-44) to 18 (BMd-43). The first two axis of the Principal Coordinates analysis (PCoA) explained 76,13% of total diversity and clearly distinguished the five groups of populations. Analysis of Molecular Variance (AMOVA) showed that 76, 20 and 4% of total diversity is among populations, individuals and within individuals, respectively. Only for multiplications carried out in Luxemburg significant differentiation between the original populations and final populations were found in two old varieties (Flageolet Chevrier Vert and Rognon de Coq, Fst = 0.117 and 0.143, respectively, P≤0.001). The results showed that in Luxemburg farmer practices and pedo-climatic conditions significantly shaped the diversity of the two populations. This is confirmed by phenotypic observations, as these multiplications also significantly differed (p<0.05) for flowering date for all varieties except 'Roi des Belges'. [less ▲]

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See detailObjective drowsiness monitoring to assess fitness for duty
François, Clémentine ULg; Hoyoux, Thomas ULg; Langohr, Thomas ULg et al

Poster (2016, September 16)

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See detailDevelopment of a semiochemical-based control method against the walnut husk fly, Rhagoletis completa Cresson
Sarles, Landry ULg; Lognay, Georges ULg; Verhaeghe, Agnès et al

Poster (2016, September 15)

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See detailExtremophile plants as source of biopesticides against European damageable plant pathogens
Ben Kaab, Sofiène ULg; Parisi, Olivier ULg; De Clerck, Caroline ULg et al

Poster (2016, September 14)

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt ... [more ▼]

The use of plant-derived products in postharvest disease management may be a valid alternative to conventional chemical treatments (Pane et al., 2016). Unfavorable environmental conditions (such as salt and drought) increase production and accumulation of reactive oxygen species (ROS). Consequently, extremophile plants have developed adaptive responses including the synthesis of specific bioactive molecules used for medical and nutritional purposes (Ksouri et al., 2012). In that context, the main objective of the present study was the identification of effective plant extracts and essential oils from extremophile plants against the most important plant pathogens in Europe (in term of loss, treatment necessity and/or cost). The study began with the selection of four endemic medicinal species suspected to be antimicrobial due to their wealth of phenolic and terpene compounds, such as flavonoids, phenolic acids, and coumarins (Ksouri et al., 2012). Each of the aerial plant materials was grounded and macerated with solvent (methanol or chloroform) for 24 h. The solvent was then eliminated along rotavapor. The yield of plant extract varied between 1.56 and 6.7%. Kinetics of growth of the 3 pathogens cultivable in liquid medium was determined before testing the impact of plant extracts and essential oils. Methanolic and chloroform plant extracts (EM1, EM2, EC1 and EC2) and essential oils (EO1, EO2) were compared for their antifungal potential. The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts was analyzed by GC/MS. Yield of essential oil varied between 0.7 and 1.2%. Therefore, antifungal activity of plant extracts and essential oils was evaluated using ELISA microplates with a blocked randomized design, as described previously (Parisi et al., 2013). The results obtained showed that EM2 at 7 mg/ml has a very high fungistatic activity against Fusarium culmorum, F. oxysporum and Penicillium italicum. It was characterized with a high amount of polyphenols, flavonoids and condensed tannins. Statistical analysis showed that the efficiency of methanol extracts significantly differed from those of the chloroform extracts. In addition, essential oils significantly reduced spores germination in a dose-dependent manner. Their fungistatic activity reached 100% at 6000 ppm. In conclusion, this work allowed us to open new perspectives on the application of extremophile plant extracts as novel biocontrol strategy against plant pathogens. [less ▲]

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See detailFirst come first served: “priority effect“ benefits Ambrosia artemisiifolia L. more than other ruderal Asteraceae species
Ortmans, William ULg; Mahy, Grégory ULg; Monty, Arnaud ULg

Poster (2016, September 14)

Ambrosia artemisiifolia L. (common ragweed, Asteraceae) is an invasive weed causing a health crisis in Europe, due to its highly allergenic pollen. In Western Europe the invaded range covers most of ... [more ▼]

Ambrosia artemisiifolia L. (common ragweed, Asteraceae) is an invasive weed causing a health crisis in Europe, due to its highly allergenic pollen. In Western Europe the invaded range covers most of central and southern France, and northern Italy. Northwards beyond the edge of this range, occurrence of casual population have been described for years, but these populations do not appear to become invasive, and the species does not seem to spread. This situation raises the following question: Has the invaded range reached a limit or will the species continue its invasion northwards? To answer this question, we followed two complementary approaches. First we set up an experimental garden in Belgium, 250 km north to the current invaded range, to see if the local climate allows the completion of the species reproduction cycle. Second, we performed an in situ measurement campaign in 12 population located beyond the edge, within the range but near the margin, and in the center of the invaded range. The aim of this campaign was to test whether the species had reduced plant performance towards range margins. The results showed that the species is able to establish populations with high growth rates in Belgium. Furthermore, the species expressed similar performance across the considered areas, even beyond the current invasion front. No evidence of processes constraining the invasion was found, which suggests a great potential for invasion north to the current invaded range. In this uncertain situation, awareness actions should be considered in the northern countries. [less ▲]

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See detailEffects of seed traits variation on seedling performance of the invasive weed, Ambrosia artemisiifolia
Monty, Arnaud ULg; Mahy, Grégory ULg; Ortmans, William ULg

Poster (2016, September 14)

Seedling performance can determine the survival of a juvenile plant and impact adult plant performance. Understanding the factors that may impact seedling performance is thus critical, especially for ... [more ▼]

Seedling performance can determine the survival of a juvenile plant and impact adult plant performance. Understanding the factors that may impact seedling performance is thus critical, especially for annuals, opportunists or invasive plant species. Seedling performance can vary among mothers or populations in response to environmental conditions or under the influence of seed traits. However, very few studies have investigated seed traits variations and their consequences on seedling performance. Specifically, the following questions have been addressed by this work: 1) How the seed traits of the invasive Ambrosia artemisiifolia L. vary among mothers and populations, as well as along the latitude; 2) How do seed traits influence seedling performance; 3) Is the influence on seedlings temperature dependent. With seeds from nine Western Europe ruderal populations, seed traits that can influence seedling development were measured. The seeds were sown into growth chambers with warmer or colder temperature treatments. During seedling growth, performance-related traits were measured. A high variability in seed traits was highlighted. Variation was determined by the mother identity and population, but not latitude. Together, the temperature, population and the identity of the mother had an effect on seedling performance. Seed traits had a relative impact on seedling performance, but this did not appear to be temperature dependent. Seedling performance exhibited a strong plastic response to the temperature, was shaped by the identity of the mother and the population, and was influenced by a number of seed traits. [less ▲]

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See detailSynthesis and characterizations of non-isocyanate polyurethane (NIPU) hydrogels
Gennen, Sandro ULg; Grignard, Bruno ULg; Thomassin, Jean-Michel ULg et al

Poster (2016, September 12)

Polyurethane (PU) is on of the most used polymers for the preparation of hydrogels due to its good biocompatibility, biodegradation and excellent mechanical properties. PU hydrogels are found in lot of ... [more ▼]

Polyurethane (PU) is on of the most used polymers for the preparation of hydrogels due to its good biocompatibility, biodegradation and excellent mechanical properties. PU hydrogels are found in lot of applications such as wound dressing, soft contact lenses, drug delivery and scaffolds for tissue engineering. Classicaly, PU is produced by a step-growth polymerization between diols and diisocyanates. However, in order to avoid the use of harmful isocyanates compounds and because of regulations which tend to ban the use of isocyanates, we developed hydrogels based on a non-isocyanate polyurethane (NIPU) chemistry by valorizing CO2-sourced cyclic carbonates and amines. Precisely, NIPU hydrogels were prepared by a solvent-free copolymerization between bifunctional hydrophilic polyethylene glycol cyclic carbonates and diamines in presence of a triamine as a crosslinker, followed by a water swelling of the obtained cross-linked gel. Parameters such as the cross-linking ratio and diamine’s nature were optimized. Different clay contents (cloiste 30B) as nanofiller were dispersed in the ideal cyclic carbonate/diamine/triamine formulation prior polymerization in order to reinforce the compression properties of NIPU hydrogels. Finaly, we were able to prepare NIPU hydrogels with water content up to 80 % and good compression properties using low clay content. [less ▲]

Detailed reference viewed: 63 (4 ULg)
See detailOrganocobalt complexes as source of radicals for the controlled polymerization of unconjugated monomers
Demarteau, Jérémy ULg; Cordella, Daniela ULg; Kermagoret, Anthony et al

Poster (2016, September 12)

Detailed reference viewed: 13 (2 ULg)
See detailPolyphosphoester containing amphiphilic block copolymers as drug nanocarriers
Ergül, Zeynep ULg; Vanslambrouck, Stéphanie; Thiry, Justine ULg et al

Poster (2016, September 12)

The design of drug delivery systems often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, supramolecularly self-assembled ... [more ▼]

The design of drug delivery systems often requires biodegradable and biocompatible materials that allow safe retention and controlled release of the drug. In this respect, supramolecularly self-assembled amphiphilic block copolymers into spherical micelles are appropriate carriers for poorly soluble drugs. In that framework, we have designed novel functional poly(ethylene oxide)-b-polyphosphoester amphiphilic block copolymers able to cross-linked under UV and degrade in response to a reduction of the pH from neutral conditions. Therefore, an unsaturated alkene side-chain was introduced on the cyclic phosphate monomer according to a one-step reaction followed by its organocatalyzed polymerization initiated by a poly(ethylene oxide) macroinitiator. After self-assembly into water, the micelles were cross-linked by UV irradiation. Then, these cross-linked micelles have been loaded by doxorubicin, i.e. a drug used in cancer therapy. We observed that the doxorubicin loading increased with the number of double bonds on the polyphosphate block of non-cross-linked micelles. This diblock amphiphilic copolymer bearing pendant unsaturations appears thus particularly promising candidate to build micellar drug delivery systems for intravenous injection. [less ▲]

Detailed reference viewed: 24 (2 ULg)