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See detailCHEMOKINE (CC-MOTIF) LIGAND 2 AS A PROGNOSTIC SERUM MARKER IN CANINE IDIOPATHIC PULMONARY FIBROSIS
Roels, Elodie ULg; Holopainen, S.; Teske, Erik ULg et al

Poster (2015, September 11)

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See detailRegional occurence of greenhouses gases in groundwater: Initial results in shallow Belgian aquifers.
Hakoun, Vivien ULg; Gesels, Julie ULg; Tseng, Jean Hsiao-Chun et al

Poster (2015, September)

Currently, the lack of robust, context-distributed subsurface greenhouses gases (GHG) concentrations data is a key bottleneck to reduce the uncertainty range of GHG groundwater input to continental ... [more ▼]

Currently, the lack of robust, context-distributed subsurface greenhouses gases (GHG) concentrations data is a key bottleneck to reduce the uncertainty range of GHG groundwater input to continental surface water bodies such as rivers or lakes estimates. Carbon dioxyde (CO2), methane (CH4) and nitrous oxyde (N2O) are likely to be indirectly transferred to the atmosphere through groundwater discharge into continental surface water bodies. We aim to extend regional-scale estimates of indirect GHG emissions by screening, in numerous hydrogeological (such as alluvial, sandstone, chalk and limestone aquifers) and land use contexts (such as industrial and agricultural), the occurence of these gases. Here, we report and discuss CO2, CH4 and N2O concentrations from an initial survey conducted over selected sites (n= 40) within shallow (0-100 m depth) aquifers in Wallonia (Belgium) for the first time. The preliminary results obtained in this study show that the range of GHG concentrations varies between 5160 and 47544 ppm, 0 and 1064 nmol.L-1, as well as 1 and 5637 nmol.L-1 for the partial pressure of CO2, CH4 and N2O respectively. This new and unique regional dataset provides a first step in developping a refined understanding of favorable contexts for GHG occurence in groundwater which may be used to reduce the uncertainties related to indirect emissions of GHG through groundwater-surface water transfers. [less ▲]

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See detailExploring the schizophrenic hyperreflexivity on the Rorschach test
Englebert, Jérôme ULg; Bataille, Jennifer; Mormont, Christian ULg et al

Poster (2015, August 28)

L’hyper-réflexivité schizophrénique peut être définie comme une attitude particulière de la conscience consistant à interroger de façon explicite et réflexive des phénomènes généralement implicites et ... [more ▼]

L’hyper-réflexivité schizophrénique peut être définie comme une attitude particulière de la conscience consistant à interroger de façon explicite et réflexive des phénomènes généralement implicites et préréflexifs (Sass, 2014). Notre hypothèse est que ce mécanisme se manifeste dans les protocoles du test de Rorschach des sujets schizophrènes (Englebert, 2013). Pour l’éprouver, nous avons administré le test à douze sujets schizophrènes. L’originalité de cette recherche repose sur une seconde présentation des planches (trois jours plus tard) qui a permis d’évoquer avec les sujets la permanence de leurs perceptions et de discuter de l’acception commune de celles-ci (pensez-vous que la plupart des autres personnes pourrait voir ce que vous voyez ?). Les résultats confirment notre hypothèse, tant au niveau quantitatif (les marqueurs de l’hyper-réflexivité que nous avions identifiés se retrouvent dans la majorité des protocoles) que qualitatif (phrases suggérant une interrogation épistémologique du test, par exemple). Enfin, nous remarquerons que l’attitude schizophrénique envers le test de Rorschach est assez subtile puisqu’elle identifie le fondement épistémologique du test reposant sur la nécessité d’une fausse perception – une « violation de la réalité » selon Exner (2003). Les sujets schizophrènes mettent en évidence la règle implicite sur laquelle repose le test qui consiste à ne pas donner la seule véritable bonne réponse (systématique) au test qui est « une tache ». [less ▲]

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See detailNegative Relative Clauses in Late Egyptian. A Functional Perspective
Sojic, Nathalie ULg

Poster (2015, August 26)

The poster contains a functional taxonomy of the negative relative clauses in Late Egyptian

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See detailDo Triclosan affect hearing development of Cyprinodon variegatus larvae?
Schnitzler, Joseph ULg; Benichou, Farida; Pinte, Nicolas et al

Poster (2015, August)

The aquatic environment represents the final sink for many chemicals, including bactericidal agents. Among them Triclosan (TCS) has been shown to affect the thyroid system of teleost. Larval stages are ... [more ▼]

The aquatic environment represents the final sink for many chemicals, including bactericidal agents. Among them Triclosan (TCS) has been shown to affect the thyroid system of teleost. Larval stages are particularly vulnerable to deleterious effects of endocrine disrupters because of potential impairment of fish development and behaviour. Thyroid hormones are critical to the development of the brain and auditory system. Thus, TCS could affect the development of the brain and hearing. The aims of this study were: to investigate hearing development in sheepshead minnows (Cyprinodon variegatus) using the ABR technique (Auditory Brainstem Response) and to investigate the effects of triclosan on hearing development. Exposure to TCS was conducted from fertilization of eggs on at concentrations likely to be found in the environment: 20, 50 and 100 µgl-1. We characterized previously the ontogenic variation of thyroid hormones in embryos and larvae of sheepshead minnows. We observed an increase of thyroid hormones level around the 12th and the 15th day post hatching (dph), that may be associated with the transition from larval to juvenile stage during the development of this species. We concluded, that this period could be defined as a critical exposure window to pollutants. We determined hearing thresholds for sheepshead minnows of different ages. Our sheepshead minnows show ontogenic variations in the hearing ability during their development. At 30 days post hatching, their hearing ability is quite bad, with a narrow bandwidth of detected frequencies. But their hearing ability considerably enhance during their development to reach the adult hearing ability at around 80 days post hatching when this species reach sexual maturity. So we observe during the developmental phase of this fish species clear ontogenic improvements of the hearing ability and they showed an ontogenetic expansion in the frequency bandwidth they were able to detect. The effects of TCS in this development have yet to be determined but will be fully discussed. This study proposes an interesting new endpoint in thyroid disruption research. [less ▲]

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See detailSedimentological and geochemical evidence to detect arid periods recorded in wadi deposits: a case study from northern Morocco
El Ouahabi, Meriam ULg; Bartz, Melanie; Rixhon, Gilles et al

Poster (2015, July 27)

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See detailIdentification of VZV ORF9p potential cellular partners that could be important for the viral egress.
Lebrun, Marielle ULg; riva, laura; Rambout, Xavier ULg et al

Poster (2015, July 26)

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate ... [more ▼]

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate of the viral kinase ORF47p and that its ORF47p-dependent phosphorylation is important for the secondary envelopment process. We also have identified an acidic cluster (AC) within the protein that is important for its correct localization in the infected cells and for the interaction with ORF47p. The recombinant VZV expressing ORF9p-ΔAC presents an accumulation of capsids in the perinuclear space. ORF9p seems then to play an important role in several steps of the egress process. In this context, we sought to identify cellular partners of ORF9p that might be important for these functions. We performed a yeast two hybrid screen against the human ORFeome 5.1. and picked out 44 candidates among which 5 proteins playing roles in membrane organization and targeting. We currently are trying to confirm these interactions in infected cells and to assess the role of these interactions for the viral lytic cycle. [less ▲]

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See detailExploratory study of narratives in children with attention deficit hyperactivity disorder
Boulard, Aurore ULg; Pignatiello, Laure ULg; Leclercq, Cédric

Poster (2015, July 21)

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See detailCombining mindfulness and ACT to learn how to manage emotions and to engage in valued activities: Assessment of the feasibility of a training group and its efficiency
Wagener, Aurélie ULg; Dierickx, Christophe; Blairy, Sylvie ULg

Poster (2015, July 18)

BACKGROUND Managing painful emotions could be an issue for a lot of adults. Indeed, learning how to manage painful emotions is an often-requested demand in psychotherapy. Mindfulness-based programs and ... [more ▼]

BACKGROUND Managing painful emotions could be an issue for a lot of adults. Indeed, learning how to manage painful emotions is an often-requested demand in psychotherapy. Mindfulness-based programs and acceptance and commitment therapy (ACT) offer useful tools which aim to guide in this learning process. ACT also provide guidelines to engage in valued activities. In order to help community adults to manage their painful emotions and to engage themselves in valued activities, we offer training group cycles combining tools of mindfulness-based programs and ACT. This study aims to assess the feasibility of such training groups and their efficiency. METHODS A longitudinal design with three assessment-times (T0, T1 and T2) is employed. Participants to the training group attend to three three-hours training sessions on a six-weeks period. They are assessed before the training sessions (T0), directly after (T1) and at three-months follow-up (T2). Questionnaires assess these variables: sociodemographic data, mood, mindfulness, psychological flexibility, cognitive coping strategies and behavioural activation. Student t tests for paired samples are conducted. RESULTS Fifty-four adults participated to four training cycles (the cycles are all identical). Until now, 28 participants (21 women) completed the T0 and the T1 (mean age = 41.43, SD = 11.83). Student t test indicate a significant decrease of depression (t(25) = 4.53, p < 0,001), anxiety (t(25) = 6.23, p < 0.001) and non-adaptive cognitive coping strategies (t(24) = 3.76, p < 0.001). Student t test also indicate a significant increase in mindfulness (t(26) = -3.87, p < 0.001), psychological flexibility (t(26)) = -5.48, p < 0.001) and behavioural activation (t(26) = -4.86, p < 0.001). DISCUSSION Preliminary results indicate the feasibility of training groups combining tools of mindfulness-based programs and ACT. Results show a decrease in several variables (depression, anxiety and non-adaptive cognitive coping strategies) and an increase in other variables (mindfulness, psychological flexibility and behavioural activation). These results seem to indicate that participants benefit from the training groups. Data collection is still ongoing and a control group (waiting-list) is currently being composed. Results and clinical implications will be further discussed during the congress. [less ▲]

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See detailMetabolomics analysis of Galium odoratum (L.) Scop.: impact of the plant population origin and growth conditions.
Ledoux, Allison ULg; Martin, Bertrand; De Tullio, Pascal ULg et al

Poster (2015, July 16)

Galium odoratum is a plant used in traditional medicine and to prepare beverages. This work aimed at studying the impact of plant origin and growth conditions on the metabolite content of the plant ... [more ▼]

Galium odoratum is a plant used in traditional medicine and to prepare beverages. This work aimed at studying the impact of plant origin and growth conditions on the metabolite content of the plant. Material and methods- Aerial biomass of Galium odoratum was collected from five natural populations (in situ conditions) and from controlled environment (ex situ conditions). Results- Quantitative analysis of selected phytochemicals including phenylpropranoids and iridoids showed clear differences between the plants from nature and those of controlled growth conditions as well as internal variation within the group. The metabolomic approach emphasized the decrease of the secondary metabolites pool paralleled by an increase of the carbohydrates in ex situ conditions. Conclusion- Metabolomics approaches using 1H-NMR and HPLC is worth to consider for studying the impact of climate factors on the regulation of the phytochemical profile in relation to the origin of the plant material. [less ▲]

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See detailDevelopmental profiles of GFAP-positive astrocytes in sheep cerebellum
Salouci, Moustafa ULg; Antoine, Nadine ULg; Shikh Al Sook, Mohamad Khir ULg et al

Poster (2015, July 16)

Astroglial cells make up the largest glial population in the brain and play a variety of vital functions in the development of the CNS. Defects in the development of these cells may induce various ... [more ▼]

Astroglial cells make up the largest glial population in the brain and play a variety of vital functions in the development of the CNS. Defects in the development of these cells may induce various neurodegenerative disorders and result in neural disease. The aim of this study is to investigate the normal development of astrocytes in sheep in various zones of the cerebellum during the different stages of gestation. 19 ovine fetuses, one lamb in the first postnatal week and three adult sheep. Sections of the cerebellum were embedded in paraffin and processed for immunohistochemistry for GFAP. The current study indicates that the first appearance of astrocytes occurs around the 8th week of gestation from restricted zones in the cerebellum. Bergmann cells were present around the 15th week of gestation. Our findings suggest that the maturation of astrocytes begins in the caudal parts of the cerebellum from their initial ventral regions to dorsal regions radially within the white matter followed by the more rostral parts of the cerebellum and that the astrocytes proliferate in the vermis before the cerebellar hemispheres. Our study provide a bases for future studies focusing on the pathological changes in the CNS of both human and sheep. [less ▲]

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See detailEarly Carboniferous events along the north-eastern Gondwanan margin: an example from the Mobarak Formation of the Central Alborz Mountains, Northern Iran.
Sardar Abadi, Mehrdad ULg; Da Silva, Anne-Christine ULg; Poty, Edouard ULg et al

Poster (2015, July 14)

The transition from the Late Devonian to the Early Carboniferous was marked by tectonic events, environmental change and global oceanic biological turnover that influenced sedimentary regimes. The end ... [more ▼]

The transition from the Late Devonian to the Early Carboniferous was marked by tectonic events, environmental change and global oceanic biological turnover that influenced sedimentary regimes. The end-Devonian faunal extinction event, for example, eliminated most of the reef building taxa, which, coupled with an increasing rate of subsidence in response to the opening of the Paleo–Tethys and a sea-ward shift of carbonate factories during the Lower Carboniferous, led to the formation of extensive carbonate ramp platforms along the Laurasian and Gondwanan margins. This development has already been reported from the western Gondwanan and southern Laurasian margins but not from northern to north-eastern Gondwanan margin yet. We aim to improve the knowledge on the main factors controlling the development and growth of carbonate ramps during the Lower Carboniferous by examining the Alborz basin along the northern margin of Gondwana. The Lower Carboniferous Mobarak Formation records the development of a storm-sensitive pervasive carbonate factory on the southern Paleo-Tethys passive margin following the opening of the Paleo-Tethys Ocean into the Alborz basin along the northern margin of Gondwana. Four outcrops in the central Alborz Mountains were studied: the Jaban, Aroo, Shahmirzad and Labnesar sections. Four events have been described as the principal factors controlling carbonate platform evolution in the Lower Carboniferous: (1) A transgression linked to global temperature rise in the Early Tournaisian (Middle Hastarain) resulted in the formation of thick-bedded argillaceous limestones. This interval is represented by massive beds of dark mudstones–wackestones to packstones that laterally interfinger with finely laminated limestones, and with bed thickness ranging on the centimeter to meter scale. This interval correlates with Lower Tournaisian nodular to argillaceous limestones of the Moravia Basin (Czech Republic), the Rhenish Slate Mountains (Poland) and the Dinant Basin (Belgium). (2) Late Hastarian–Early Ivorian glaciations were identified in Southern Gondwana but had not been evidenced in Northern Gondwana yet. This glaciation regime is recorded through the appearance of inner-ramp channel-form facies in mid– and outer-ramp settings. (3) During Late Ivorian–Early Visean?, a differentiation block faulting regime along the basin’s margin caused uplift of the westernmost parts of the Alborz basin and resulted in a sub-marine collapse in the eastern part of the central basin. This caused vast sub-aerial exposure and brecciation the top of the Mobarak Formation at the Jaban and Aroo sections. The sub-marine collapse was recorded through the occurrence of Zoophycos bearing fine-grained limestones in the top of the Labnesar and Shahmirzad sections. (4) Tectonic activity that coincided with considerable and abrupt sea level falls as an indirect consequence of the Viséan and Serpukhovian glaciations phases. This progressive sea level drop led to the stagnation of the carbonate factory, which is expressed as an erosional surface at the top of the Mobarak Formation. [less ▲]

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See detailDevelopment of a simple approach for ultrasensitive detection of bisphenols by multiplexed surface enhanced Raman scattering
De Bleye, Charlotte ULg; Dumont, Elodie ULg; Netchacovitch, Lauranne ULg et al

Poster (2015, July 13)

A very simple, cheap and fast SERS method using functionalized silver nanoparticles was developed to detect bisphenols. This method was applied for the semi-quantitative detection of bisphenol A (BPA ... [more ▼]

A very simple, cheap and fast SERS method using functionalized silver nanoparticles was developed to detect bisphenols. This method was applied for the semi-quantitative detection of bisphenol A (BPA), bisphenol B (BPB) and bisphenol F (BPF) separately. Afterwards, a feasibility study of performing a multiplex SERS detection of BPA, BPB and BPF was successfully carried out. Finally, this developed method was applied on real samples which were solutions comprising cash receipts collected from different stores. [less ▲]

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See detailMethane distributions and sea-to-air fluxes in the South China Sea and the West Philippines Sea
Tseng; Chen; Borges, Alberto ULg et al

Poster (2015, July 07)

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See detailGlucosinolates and by-products in rapeseed meal related to hydrothermal processing
Quinsac, A.; Carré, P.; Fauconnier, Marie-Laure ULg et al

Poster (2015, July)

Background: For safety reasons, rapeseed meal (RSM) is usually desolventized with strong hydrothermal treatments, leading to various levels of residual glucosinolates (GSL), and protein solubility. The ... [more ▼]

Background: For safety reasons, rapeseed meal (RSM) is usually desolventized with strong hydrothermal treatments, leading to various levels of residual glucosinolates (GSL), and protein solubility. The RSM nutritional quality may be then lowered for monogastrics, due to GSL breakdown products whose reliable and “easy to use” indicators lack. [less ▲]

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See detailTowards precision β-decay measurements with laser cooled 35Ar
Glover, Rohan ULg; Lenaers, Florence ULg; Velten, Philippe et al

Poster (2015, July)

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See detailQuasar radio polarizations align with large quasar group major axes
Pelgrims, Vincent ULg; Hutsemekers, Damien ULg

Poster (2015, July)

Using quasar polarizations and a sample of large quasar groups drawn from the SDSS DR7, we showed that at HIGH REDSHIFTS (z ~ 1.5) the spin axis orientations of black holes are correlated to the major ... [more ▼]

Using quasar polarizations and a sample of large quasar groups drawn from the SDSS DR7, we showed that at HIGH REDSHIFTS (z ~ 1.5) the spin axis orientations of black holes are correlated to the major axes of the groups to which they belong. Inside very rich groups the BH spins are aligned with the major axes. When the richness decreases, the spin axes become preferentially perpendicular. [less ▲]

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See detailDevelopment of quantification methods applied to HSS alloys for carbides volume fraction and grain size assessments
Tchuindjang, Jérôme Tchoufack ULg; Paydas, Hakan ULg; Hashemi, Seyedeh Neda ULg et al

Poster (2015, July)

High speed steels (HSS) are used in applications where enhanced mechanical properties together with hot oxidation and wear resistances are required. Such an improved physico-chemical behaviour is mainly ... [more ▼]

High speed steels (HSS) are used in applications where enhanced mechanical properties together with hot oxidation and wear resistances are required. Such an improved physico-chemical behaviour is mainly due to the presence of primary carbides within the microstructure. Depending of the initial chemical composition several types of carbides can precipitate in HSS such as MC (Nb and V-rich), M2C (Mo and W-rich), Cr-rich M7C3, etc. Two groups of HSS were analysed in this study, which are the so-called cast HSS grades that are manufactured by a centrifugal casting process and the cladded HSS manufactured by laser cladding. Purpose: Quantify the carbide volume fractions and the grain size within HSS grades in order to set correlations between the microstructure and both the mechanical and the tribological properties of the materials. Findings: The metallurgical features such as carbides nature and shape have been determined that are related to the manufacturing process of the studied material, and several methods have been used to quantify the carbide volume fractions and to assess the grain size. [less ▲]

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See detailPhotocatalytic Oxidative Treatment of Waste Water contamined with Pharmaceutical Products
Vreuls, Christelle; Wilmot, Annick; Cludts, Marc et al

Poster (2015, July)

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See detailA hydrochemical - isotopic approach for assessing factors controlling the regional pollution of an urban aquifer
Gesels, Julie ULg; Orban, Philippe ULg; Popescu, Ileana-Cristina et al

Poster (2015, June 24)

The alluvial aquifer of the Meuse River is contaminated at regional scale in the urbanized and industrialized area of Liège in Belgium, in particular inorganics pollutants such as sulfate, nitrate and ... [more ▼]

The alluvial aquifer of the Meuse River is contaminated at regional scale in the urbanized and industrialized area of Liège in Belgium, in particular inorganics pollutants such as sulfate, nitrate and ammonium. The sources of those contaminants are numerous: brownfields, urban waste water, subsurface acid mine drainage from former coal mines, atmospheric deposits related to former pollutants emissions in the atmosphere ... Sulfate, nitrate and ammonium are both typical pollutants of the aquifer and tracers of the possible pollution sources. In the Water Framework Directive context, a detailed hydrogeochemical characterization of groundwater was performed. The aim is to determine the origin of the inorganic contaminations, the main processes contributing to poor groundwater quality and the spatial extent of the contaminations. A large hydrochemical sampling campaign was performed, based on 71 selected representative sampling locations, to better characterize the different vectors (end-members) of contamination of the alluvial aquifer and their respective contribution to groundwater contamination in the area. Groundwater samples were collected and analyzed for major and minor compounds and metallic trace elements. The analyses also include stable isotopes in water, sulfate, nitrate, ammonium, dissolved inorganic carbon, boron and strontium. Different hydrogeochemical approaches are combined to obtain a global understanding of the hydrogeochemical processes at regional scale. Hydrochemical interpretations are based on classical diagrams, spatial distribution maps, geochemical equations, multivariate statistics such as self-organizing maps and isotopic analyses. With this combined approach, the location of the contaminant sources and most contaminated sectors of the alluvial aquifer together with a better understanding of geochemical processes involved are obtained. Redox processes strongly influence the composition of groundwater, specifically for compounds degrading the quality of groundwater in the area (sulfate, nitrate and ammonium). The highest concentrations of sulfate can be associated with the post-mining stage in the acid mine drainage process. Various reactions involving nitrogen compounds have been identified and allow a better understanding of causes of high concentrations of ammonium and nitrate. Denitrification and sulphate reduction are also demonstrated based on isotopic ratios. [less ▲]

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See detailMicrobial diversity and function during different bioremediation strategies of diesel-polluted soil
Masy, Thibaut ULg; Hiligsmann, Serge ULg; Thonart, Philippe ULg et al

Poster (2015, June 16)

In numerous hydrocarbon-polluted sites, oxygen and pollutant bioavailability constitutes the main limiting factors for biodegradation because of the strong adsorption of hydrocarbons on organic soil ... [more ▼]

In numerous hydrocarbon-polluted sites, oxygen and pollutant bioavailability constitutes the main limiting factors for biodegradation because of the strong adsorption of hydrocarbons on organic soil particles (clay and peat). Therefore, several strategies such as biostimulation (with air/H2O2 and/or nutrients) or bioaugmentation are used, but often without understanding the endogenous microflora degrading capacity. This lack of differentiation between indigenous and added microorganisms could lead to poor predictability of the biodegradation efficiency. In addition, anaerobic degradation remains less applied in industrial settings for such compounds (especially for saturated hydrocarbons) as this process remains slow. In this context, the main objective of our study was to understand how the bacterial community evolves, in terms of species and degrading gene diversities, during the application of three different bioremediation strategies in a heavily diesel-polluted clay soil: (i) anaerobic natural attenuation, (ii) bioventing and (iii) bioaugmentation with Rhodococcus erythropolis T902.1. In addition to the supply of new degrading genes, bioaugmentation with this biosurfactant-producing strain should facilitate the bioassimilation of desorbed hydrocarbons by the whole degrading microflora. This hypothesis is strengthened by previous results obtained during several microcosm- and pilot-scale experiments. Aerobic and anaerobic microcosms were set up with three different soil samples coming from the same polluted site. Initially, their global organic content was identical but their hydrocarbon and peat concentrations were different, which led to differential oxygen consumption. Soils were sampled every 10 days to extract the DNA to measure changes in bacterial populations (with RISA analysis and 16S rRNA gene sequencing) and function (with qPCR and sequencing of degrading genes). Further analyses of the hydrocarbon content by GC-MS and of the genetic diversity by MiSeq metagenomic analysis provided detailed chemical and functional microbial data related to compound degradation and relative gene increases. Initial results showed significant differences in the microbial community structure. Moreover, Rhodococci seem to be maintained in the soil after inoculation. [less ▲]

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See detailModélisation de la mortalité de larves d'Hylotrupes bajulus (L.) par traitement anoxique pour la conservation d'objets d'intérêt patrimonial
de Streel, Géraud; Henin, Jean-Mac; Mercier, Emmanuelle et al

Poster (2015, June 16)

Au cours des dernières décénnies, les preuves scientifiques concernant les dangers des insecticides utilisés pour éliminer les insectes présents dans les œuvres d’art des points de vue de la santé du ... [more ▼]

Au cours des dernières décénnies, les preuves scientifiques concernant les dangers des insecticides utilisés pour éliminer les insectes présents dans les œuvres d’art des points de vue de la santé du personnel et des visiteurs, des risques environnementaux et des risques pour les œuvres ont conduit le monde de la conservation patrimoniale à se tourner vers des techniques alternatives de désinsectisation. Le traitement par anoxie fait partie de ces techniques. Il s’agit de soumettre les insectes à une atmosphère à faible teneur en oxygène (généralement < 0.1%) pendant une certaine durée pour les éliminer sans causer de dégât à l’œuvre ni laisser de résidus toxiques. Cette technique souffre toutefois de deux inconvénients majeurs. Le premier concerne l’importante durée de traitement nécessaire et le second concerne la sensibilité des résultats à un grand nombre de paramètres environnementaux. Cette étude a pour but d’analyser l’impact des températures élevées comme moyen pour accélérer le traitement ainsi que d’établir des modèles de mortalité intégrant certain paramètres qui influencent l’efficacité du traitement en vue de faciliter la détermination de la durée de ce dernier. Cette étude est réalisée en collaboration avec l’Institut Royal du Patrimoine Artistique (IRPA), et a également pour but de leur fournir un protocole permettant d’assurer une efficacité de traitement proche de 100%. Il a été montré que, conformément à ce qui existe dans la littérature, la température, la durée de traitement et le poids initial des larves influencent significativement la probabilité de mortalité des larves soumises au traitement tandis que le fait que la larve se situe dans une boite de Pétri ou dans une planchette de bois ne joue pas de rôle significatif. Il est également mis en évidence que, si la déshydratation des insectes lors du traitement joue un rôle important sur la mortalité, ce n’est pas le seul mécanisme impliqué. Des recherches supplémentaires sont nécessaires à ce sujet. Des modèles ont également pu être établis et ils permettent de déterminer qu’à température ambiante et 50% d’humidité du flux de gaz, la durée de traitement nécessaire pour éliminer 99% des insectes est de 26 jours [less ▲]

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See detail‘Ex-situ’ preservation and characterization of Antarctic cyanobacteria in the BCCM/ULC collection
Kleinteich, Julia ULg; Renard, Marine ULg; Simons, Véronique et al

Poster (2015, June 09)

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. An ISO9001 certificate was obtained for the public deposition and distribution of ... [more ▼]

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. An ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. BCCM/ULC is currently holding 160 public cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/catalogues/ulc-catalogue-search. Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. The main holding of the collection concerns (sub)polar strains isolated from different biotopes and representative of a large taxonomic diversity. The molecular characterization is underway, on the basis of 16S rRNA and ITS sequences, but also Multiple Locus Sequence Analysis and genome sequencing. In addition, cyanobacteria are known to produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with various bioactive potential. The presence of genes involved in the production of microcystin is currently studied by PCR, and analytical methods are used to confirm the toxin production. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems especially promising for biotechnology or biomedical applications. [less ▲]

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See detailStudying the great potential of cultivable bacteria associated with the brown alga Ascophyllum nodosum
Martin, Marjolaine ULg; Martin, Renée ULg; Barbeyron, Tristan et al

Poster (2015, June 09)

Bacteria associated with algae differ markedly from those living freely in seawater and represent great potential for the production of diverse bioactive compounds as they interact in multiple complex ... [more ▼]

Bacteria associated with algae differ markedly from those living freely in seawater and represent great potential for the production of diverse bioactive compounds as they interact in multiple complex ways with their host. Here we identified new bacterial species, and their polysaccharolytic activities, associated with the brown alga Ascophyllum nodosum. To isolate cultivable microorganisms, algal thalli of Ascophyllum nodosum were swabbed with sterile cotton tips and marine agar plates were inoculated. Three-hundred isolated bacteria were screened for agarase, kappa- or iota-carrageenase activities on specific marine media. Thirty-two bacteria with polysaccharolytic activities were isolated and a part of their 16S rDNA (8F-1492R) were amplified and sequenced. Twenty-seven were classified as Flavobacteriia and five as Gammaproteobacteria. Putative new strains and species of Zobellia, Maribacter, Cellulophaga, Shewanella, Glaciecola, Pseudoalteromonas and Colwellia were identified by phylogenetic analysis. Genomics libraries with their DNA were constructed in Escherichia coli and Bacillus subtilis and are currently screened for diverse enzymatic activities (agarases, iota-and kappa-carrageenases, cellulases, beta-glucosidases, sulfatases and amylases). In an era where high throughput sequencing is mostly used to study bacterial communities, cultivation methods are underestimated. Here, we revealed that only ten percent of the cultivable bacteria on this brown alga could degrade algal polysaccharides, which lead to asking us; who and what are the 90 other percents doing there? Furthermore, by this cultivation method we could also identify putative new bacterial strains/species, which are screened for polysaccharidases. Novel glycoside hydrolases from unknown marine bacteria represent great biotechnological potential as they should have original industrial properties. [less ▲]

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See detailTowards the identification of mechanisms underlying the high expression of HMA4, an essential gene for zinc hyperaccumulation in Arabidopsis halleri.
Spielmann, Julien ULg; Nouet, Cécile ULg; Scheepers, Maxime ULg et al

Poster (2015, June 08)

To maintain metal homeostasis within physiological limits independently of metal concentrations present in soil, plants developed a complex network including metal uptake, chelation, trafficking, and ... [more ▼]

To maintain metal homeostasis within physiological limits independently of metal concentrations present in soil, plants developed a complex network including metal uptake, chelation, trafficking, and storage processes. In this network, transporters have a central role. To study the molecular basis of zinc homeostasis in plants, we are using A. halleri, a zinc- and cadmium-tolerant and zinc-hyperaccumulating species representing the extreme end of natural variation in terms of metal homeostasis. Indeed, A. halleri stores high amount of metals in vacuoles of above-ground tissues (>1% Zn or 0.01% Cd in shoot dry weight). Cross-species transcriptomic studies identified about thirty genes which are constitutively over-expressed in A. halleri compared to its non-tolerant and non-hyperaccumulator sister species, Arabidopsis thaliana (1-3). HMA4 is one of these genes. It encodes a Zn/Cd plasmamembrane pump involved in xylem loading/unloading which is a key step in the hyperaccumulation process (4-5). The HMA4 overexpression is determined by a combination of gene triplication and cis-regulatory changes increasing transcription levels of each three HMA4 copies (5). We aim to identify the cis-elements underlying high expression of HMA4 in A. halleri using promoter-reporter constructs. [less ▲]

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See detailA lysine cluster in domain II of Bacillus subtilis PBP4a plays a role in the membrane attachment of this C1 PBP.
Vanden Broeck, Arnaud; Dauvin, Marjorie ULg; Sauvage, Eric et al

Poster (2015, June 08)

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114 ... [more ▼]

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114, K119 and K265 have been mutated in glutamine residues (Q). Both proteins (WT and Mut4KQ PBP4a) have been produced without signal peptide in E. coli and their sub-cellular localizations determined by measuring the DD-carboxypeptidase activities in the different compartments (cytoplasmic vs membrane attached proteins). After purification, their binding to B. subtilis membranes has been compared: WT PBP4a interacts in vitro with membranes isolated from this organism in contrast to Mut4KQ PBP4a that remains entirely unbound. In absence of any amphiphilic peptide in PBP4a, the crown of positive charges on the surface of domain II is likely responsible for the PBP cellular localization in interaction with the cytoplasmic membrane. [less ▲]

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See detailExpression study of FRD3 in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Spielmann, Julien ULg et al

Poster (2015, June 08)

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene ... [more ▼]

Transcriptomic studies identified genes which are constitutively over-expressed in A. halleri compared to A. thaliana and which may have a role in metal tolerance or accumulation (1-3). A candidate gene encodes FRD3, a member of the MATE family of membrane transporters (56 members in A. thaliana). It is a citrate transporter involved in iron homeostasis (4-6) and playing a role in zinc tolerance in A. thaliana (7). We are aiming to analyse the FRD3 high expression in A. halleri and the FRD3 function in zinc and iron homeostasis in A. thaliana. [less ▲]

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See detailUNRAVELLING THE ROLES OF LYSINE ACETYLATION BY ELP3 DURING INNER EAR DEVELOPMENT
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Freeman, Stephen ULg et al

Poster (2015, June 06)

Given the importance of acetylation homeostasis in controlling developmental processes [1-3], we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase ... [more ▼]

Given the importance of acetylation homeostasis in controlling developmental processes [1-3], we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a member of the Elongator complex recently implicated in neurogenesis [4]. To determine the role of Elp3 in the inner ear, we first analysed the spatio-temporal pattern of ELp3 mRNA expression and showed that it was expressed in the entire early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea (the organ of Corti), in the spiral ganglion, in the stria vascularis and in the vestibule. To unravel in vivo functions of Elp3 in the inner ear, we used conditional knock-out mice in which Elp3 gene is deleted from early otocyst (Elp3 cKO). We submitted these mice to a battery of vestibular testing (i.e. stereotyped circling ambulation, head bobbing, retropulsion, and absence of reaching response in the tail-hanging test) and found significant abnormalities. Besides, the auditory brain stem response of Elp3 cKO indicated that these mice are severely deaf. At the cellular level, we did not find any structural abnormalities nor cell patterning defects that could explain deafness or balance dysfunction in Elp3 cKO mice. However, we detected some defaults in the planar orientation of their auditory hair cell bundle. We were also able to demonstrate an increased level of apoptosis in the Elp3 cKO spiral ganglion at E14.5 leading to a reduced number of neurons and fibers innervating the cochlear hair cells as well as a reduced number of their synaptic ribbons at P15. Moreover, the remaining spiral ganglion neurons extend processes showing clearly defects regarding hair cells innervation (misorientation of fibers). In conclusion, our results clearly show a role for Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins that are targeted for acetylation by Elp3. [less ▲]

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See detailCXCR7/ACKR3 is activated by chemokines from both CXC and CC subfamilies
Szpakowska, Martyna ULg; Counson, Manuel; Beaupain, Nadia et al

Poster (2015, June 05)

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See detailDesign and synthesis of PET-probes targeting AMPA-subtype receptors
Deverdenne, François ULg; Claes, Giselle ULg; Goffin, Eric ULg et al

Poster (2015, June 05)

The AMPA subtype of glutamatergic receptors is the main actor in the excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the ... [more ▼]

The AMPA subtype of glutamatergic receptors is the main actor in the excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the maintenance of the long-term potentiation, a phenomenon closely linked to cognitive and memorization processes. Based on experimental data collected in recent years, the use of AMPA potentiators seems to be an interesting approach in the treatment of cognitive deficits (e.g. Alzheimer disease), schizophrenia or depression. Such AMPA signal potentiation could be mediated by positive allosteric modulators (PAMs) of the AMPA receptors, a class of compounds able to produce a fine signal tuning in the presence of the endogenous ligand in the synapse, providing less toxicity than direct agonists. With this approach, the laboratory of Medicinal Chemistry of Liège university developed many series of AMPA potentiators , among which 1,2,4-benzothiadiazine 1,1-dioxides (BTDs). In order to better understand the in vivo mapping of AMPA receptors and its evolution in neurological diseases, the present work aims at developing the design and the synthesis of BTDs positive allosteric modulators radiolabeled with a fluorine-18 atom. Based on previously synthesized series in this field, we investigate the synthesis of a new class of high-affinity AMPA potentiators characterized by the presence of a fluorine atom at selected positions on the structure of the AMPA potentiators. Thanks to in vitro pharmacological evaluations, we will further determine the best candidates for their fluorine-18 radiolabeling. [less ▲]

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See detailSpatio-temporal dynamics of β-tubulin isotypes and acetyl-alpha tubulin during the development of the sensory auditory organ in rodents.
Renauld, Justine ULg; Thelen, Nicolas ULg; Johnen, Nicolas ULg et al

Poster (2015, June 05)

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These ... [more ▼]

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These cells are supported by supporting cells. Supporting cells possess a characteristic cytoskeleton in direct relation with their morphological features and their development. There are different β-tubulin isoforms in microtubules of vertebrate tissues. However, their functional significance is still largely unknown. In the present study, we investigated the localization of five β-tubulin isotypes (β 1 to 5) as well as acetyl-α-tubulin within the hearing organ during development in rodents. By using confocal microscopy, we showed that with the exception of the β3-tubulin isoform that was specific to nerve fibres, all the different β-tubulin isoforms and acetyl-α-tubulin were mainly present in the supporting cells. Contrary to β1-4-tubulins, we also found that the β5-tubulin isoform appeared only at a key stage of the postnatal development in specific cell types (pillar cells and Deiters’ cells). By using transmission electron microscopy, we revealed further that this developmental stage coincided with the formation of two separate bundles of microtubules from a unique one in these supporting cells. In conclusion, these results suggest that the β5-tubulin isoform might be involved in the generation of new microtubule bundles from a pre-existing one. [less ▲]

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See detailThe Main Auroral Emission at Jupiter: Altitude profile and Dawn-Dusk Asymmetry
Bonfond, Bertrand ULg; Gustin, Jacques ULg; Gérard, Jean-Claude ULg et al

Poster (2015, June 04)

The main auroral emission at Jupiter generally forms a quasi-continuous curtain around each magnetic poles. This emission magnetically maps to the middle magnetosphere and is related to the corotation ... [more ▼]

The main auroral emission at Jupiter generally forms a quasi-continuous curtain around each magnetic poles. This emission magnetically maps to the middle magnetosphere and is related to the corotation enforcement of the plasma originating from the volcanic satellite Io. The first models of corotation enforcement current system at Jupiter assumed symmetry around the magnetic axis. However, observations and further development of these models outlined the importance of local time variability of such currents. In this presentation, we show the results of two studies of this local time variability relying on the large dataset of Far-UV observations from the Hubble Space Telescope (HST). Knight’s theory of field aligned current predicts that the auroral precipitating energy flux and the energy of the precipitating electrons are correlated. Since the altitude of the auroral emissions decreases as the energy increases, it is thus expected that the altitude of the auroral brightness peak varies as a function of the local time following the variations of the field aligned currents. We compare the altitude of the main emission on the post-dusk side as seen in the visible domain by Galileo’s Solid State Imager and the same altitude for the night side as seen by the Advanced Camera for Surveys (ACS) on board HST in the Far-UV domain. We show some significant differences between the two data sets. Unfortunately, a careful analysis involving both spectral observations and simulations indicates that the Far-UV vertical profiles are hampered by observational ambiguities due to absorption by hydrocarbon molecules. Only additional and judiciously designed new observations could reveal the actual amount of methane along the line of sight. The second study consists in a comparison of the emitted power in local time sectors corresponding to dawn and dusk. Results in the northern hemisphere are difficult to interpret because the magnetic anomaly probably causes a decrease of the auroral brightness in regions of strong magnetic field. In the southern hemisphere, where the field magnitude is more uniform along the main oval, the dusk sector is ~3 times brighter than the dawn sector. In accordance with measurements of magnetic field divergence in the equatorial plane by Galileo, these results suggest the presence of a partial ring current in the night side of the magnetosphere with upward currents in the dawn side and downward currents in the dusk side. [less ▲]

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See detailCritical periods and critical values explaining fluxes inter-annual variability in a temperate mixed forest
Hurdebise, Quentin ULg; Vincke, Caroline; De Ligne, Anne ULg et al

Poster (2015, June 04)

In order to explain inter-annual variability of Net Ecosystem CO2 Exchange (NEE) above a mixed temperate forest, two approaches were followed: •Detection of critical periods using the R-squared of the ... [more ▼]

In order to explain inter-annual variability of Net Ecosystem CO2 Exchange (NEE) above a mixed temperate forest, two approaches were followed: •Detection of critical periods using the R-squared of the regression between annual NEE and cumulated NEE on a mobile window. •Identification of critical values of a threshold used to decompose annual and seasonal NEE in two components. [less ▲]

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See detailBIOREFINE Project – Recovery of useful nutrients from organic wastes produced in five European countries (3AV.3.55)
Tarayre, Cédric ULg; De Clercq, Lies; Michels, Evi et al

Poster (2015, June 01)

At this time, many wastes are used or eliminated through processes that do not really consider their potential applications. Such wastes contain useful nutrients (nitrogen, phosphorus and potassium), the ... [more ▼]

At this time, many wastes are used or eliminated through processes that do not really consider their potential applications. Such wastes contain useful nutrients (nitrogen, phosphorus and potassium), the importance of which has been demonstrated in agriculture for many years. The composition of wastes is highly heterogeneous, which makes treatment techniques more difficult to apply on a large scale. Sewage sludge is usually used as a fertilizer in agriculture, in energy production or in the field of construction. The main use of manure is agriculture, although considerable amounts of nutrients are lost and cause pollution. Digestate is also used in agriculture, but other alternatives have been proposed. Ashes should also be highlighted, although they do not contain nitrogen, which is lost into the atmosphere during the combustion process. Finally, household and industrial wastes are resources that should be considered as well. Those different types of wastes could be recycled to produce environment-friendly fertilizers. Here, we propose to investigate these opportunities inside five European countries (Belgium, France, Germany, United Kingdom and The Netherlands) through five work packages with the BioRefine Project. [less ▲]

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See detailHubble Space Telescope observations of variation of the O I 135.6 nm/ O I 130.4 nm ratio in Ganymede’s atmosphere
Molyneux, P. M.; Nichols, J. D.; Bannister, N. P. et al

Poster (2015, June)

We present new high-sensitivity HST/COS measurements of the atmospheric O I 135.6 nm/ O I 130.4 nm ratio at Ganymede, which we show exhibits significant spatial and temporal variability. Specifically, the ... [more ▼]

We present new high-sensitivity HST/COS measurements of the atmospheric O I 135.6 nm/ O I 130.4 nm ratio at Ganymede, which we show exhibits significant spatial and temporal variability. Specifically, the ratios observed on Ganymede’s leading hemispheres vary between 2.14±0.03 and 2.67±0.02, while on the trailing hemisphere the ratios are observed to be between 0.98±0.02 and 1.53±0.03. These high-sensitivity observations increase the signal to noise of these measurements by an order of magnitude over previous HST/STIS observations of the same [1], thus confirming that the temporal variation suggested by these previous observations is real. The emissions are excited through electron-impact excitation of Ganymede’s oxygen atmosphere by electrons which are locally accelerated within its magnetosphere [2,3]. The variation in the ratio magnitude may be explained either by variations in the ratio of atomic to molecular oxygen in the atmosphere or by a change in the temperature of the electrons exciting the emissions. An increase in the proportion of molecular oxygen acts to increase the ratio, as does a cooler electron temperature.References [1] Feldman, P. D., McGrath, M. A., Strobel, D. F., Moos, H. W., Retherford, K. D. and Wolven, B. C., HST/STIS ultraviolet imaging of polar aurora on Ganymede, Astrophys. J., Vol. 535, pp. 1085-1090, 2000. [2] Hall, D. T., Feldman, P. D., McGrath, M. A. and Strobel, D. F., The far-ultraviolet oxygen airglow of Europa and Ganymede, Astrophys. J., Vol. 499, pp. 475-481, 1998. [3] Eviatar, A., Strobel, D. F., Wolven, B. C., Feldman, P. D., McGrath, M. A. and Williams, D. J., Excitation of the Ganymede ultraviolet aurora, Astrophys. J., Vol. 555, pp. 1013-1019, 2001. [less ▲]

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See detailSearch for Satellite Effects on Saturn's Auroras in Cassini UVIS Data
Pryor, Wayne; Espositio, Larry; Jouchoux, Alain et al

Poster (2015, June)

The Cassini Ultraviolet Imaging Spectrograph (UVIS) has been obtaining Saturn auroral images since 2004. We have previously reported instances when the main auroral oval brightened briefly in a quasi ... [more ▼]

The Cassini Ultraviolet Imaging Spectrograph (UVIS) has been obtaining Saturn auroral images since 2004. We have previously reported instances when the main auroral oval brightened briefly in a quasi-periodic fashion near the sub-Mimas longitude. Here we examine the large set of UVIS auroral images obtained from close range and high sub-spacecraft latitudes. We will plot the brightness of the individual auroral measurements (and binned auroral measurements) as a function of local time, and as a function of the location of Mimas and other moons to test for any correlations. Mimas, while a relatively small moon, exerts a strong influence on Saturn's ring system. Mimas creates the Cassini Division between the A and B rings and forces a non-circular shape to the outer edge of Saturn's B ring that is partially locked to Mimas phase. [less ▲]

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See detailCharacteristics of Jupiter's auroral acceleration region
Ray, Licia; Gustin, Jacques ULg; Grodent, Denis ULg

Poster (2015, June)

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. The magnetospheric drivers of the emission are relatively well understood, yet the high-latitude characteristics of ... [more ▼]

Jupiter’s dynamic auroral region is the signature of magnetosphere-ionosphere coupling. The magnetospheric drivers of the emission are relatively well understood, yet the high-latitude characteristics of the interaction have not been measure in-situ. Ahead of Juno’s arrival next summer, we use HST STIS observations of Jupiter’s auroral emission to infer the location of Jupiter’s auroral acceleration region and the properties of the precipitating auroral electrons. We analyze two images of Jupiter’s northern emission, determining the precipitating electron energy and incident energy flux for the main aurora, Io spot, Ganymede footprint, and flare regions. The resulting relationships between energy flux and electron precipitation energy for the main auroral emission are compared to the theoretical relationship derived by Lundin & Sandahl [1978] for a range of auroral region locations, and temperatures and densities appropriate for the jovian system. [less ▲]

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See detailControlled clinical trial of repeated left prefrontal transcranial direct current stimulation in patients with chronic minimally conscious state
Martial, Charlotte ULg; Thibaut, Aurore ULg; Wannez, Sarah ULg et al

Poster (2015, June)

A recent study showed that single-session anodal transcranial direct current stimulation (tDCS) applied to the left dorsolateral prefrontal cortex (LDLPF) transiently improves consciousness in 43% of ... [more ▼]

A recent study showed that single-session anodal transcranial direct current stimulation (tDCS) applied to the left dorsolateral prefrontal cortex (LDLPF) transiently improves consciousness in 43% of patients in minimally conscious state (MCS) (Thibaut et al., 2014). We here test the potential effects and safety of repeated tDCS in severely brain-damaged patients with MCS. In this double-blind cross-over sham-controlled experimental design, we delivered two sessions of repeated (5 days of stimulation) tDCS, either anodal or sham in a randomized order. We stimulated the LDLPF cortex during twenty minutes in 20 MCS patients (12 men, aged 48±16 years, time since onset 78±95 months, 12 post-traumatic). Consciousness was assessed by the French adaptation of the Coma Recovery Scale Revised (CRS-R; Schnakers et al., 2008) before and after each stimulation. A treatment effect was observed for the comparison between CRS-R total scores at baseline and after 5 days of real tDCS (p<0.01). Behaviorally, 10/20 patients showed a tDCS-related improvement; 5 patients responded after the first stimulation and 5 other patients responded after 2, 3 or 4 days of stimulation. No side effect (e.g. epilepsy) was reported. Our results demonstrate that repeated (5 days) anodal LDLPF tDCS is safe and might improve signs of consciousness in about half of patients in MCS. It is important to note that the first session is not predictive for a future positive effect of the efficacy of the non-invasive electrical stimulation. [less ▲]

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See detailGenetic depletion of the dual specificity protein phosphatase DUSP3 promotes LLC Lung tumour metastasis
Vandereyken, Maud ULg; Amand, Mathieu; Van Overmeire, Eva et al

Poster (2015, June)

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell ... [more ▼]

DUSP3, also called Vaccinia-H1 Related (VHR) is a small dual specificity phosphatase dephosphorylating both tyrosine and serine/threonine phosphorylated residues. DUSP3 plays an important role in cell cycle regulation and is up-regulated in several human cancers. The physiological role of this phosphatase is, however, poorly understood. We have recently generated a DUSP3 knockout mouse by homologous recombination. The obtained mice have no spontaneous phenotype or pathology. However, DUSP3 deficiency prevented neo-vascularization of subcutaneously transplanted Matrigel plugs and Lung Lewis Carcinoma (LLC) tumours, suggesting an involvement of DUSP3 in tumour angiogenesis. Considering the importance of angiogenesis in metastatic formation, our study aimed to investigate the role of DUSP3 in metastatic dissemination. To do so, we used the LLC experimental metastasis model that shortcuts the intravasation/extravasation processes by injecting intravenously the LLC and the B16 (metastatic melanoma cell line) cells. Surprisingly, LLC, but not B16, lung metastasis developed twice faster in DUSP3-KO than WT mice. The enhanced LLC metastatic growth in DUSP3-/- mice was transferable to WT mice via DUSP3-/- bone marrow adoptive transfer, suggesting an involvement of the hematopoietic compartment in the observed phenotype. This was confirmed by a higher infiltration of neutrophils and macrophages in the lungs of DUSP3-KO compared to WT mice after LLC injection. This infiltration was correlated with higher expression of the chemokine receptor CCR2 in LLC-bearing DUSP3-KO lungs macrophages. Interestingly, LLC, but not B16 cells, were found to secrete high level of CCL2/MCP1, the CCR ligand chemokine. In line with this observation, we found that DUSP3-/- bone marrow derived-macrophages have a higher migration potential in response to LLC, but not B16, -conditionned medium. Altogether, our results suggest that DUSP3 plays an important role in metastatic dissemination/growth by a mechanism involving the control of CCR2-CCL2 chemoattraction axis in macrophages. [less ▲]

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See detailIdentification of the main parameters controlling the plasticity of ceramic pastes: The case study of the Marrakech region (Morocco)
El Boudour El Idrissi, Hicham ULg; Daoudi, Lahcen; Fontaine, François ULg et al

Poster (2015, June)

Numerous traditional ceramic workshops occur within a range of 10 to 80 km around the city of Marrakech. Our study aims to identify the main parameters controlling the plasticity of pastes used by the ... [more ▼]

Numerous traditional ceramic workshops occur within a range of 10 to 80 km around the city of Marrakech. Our study aims to identify the main parameters controlling the plasticity of pastes used by the artisans. To reach this goal, we characterize by X-ray diffraction, granulometry and plasticity index (PI) the local raw clayey material. The investigation of 26 whole pastes shows the dominance of quartz (20 to 50%) and clay minerals (25 to 60%) with K-feldspar (2 to 17%), plagioclase (2 to 25%), calcite (0 to 18%), dolomite (0 to 15%), goethite (0 to7%) and trace of hematite and anhydrite (< 3%). Amphibole occurs in trace (< 5%) but only in some samples. The clay minerals are diversified, including illite (10 to 40%), kaolinite (2 to 15%), mixed-layer (ML) (0 to 10%), smectite, vermiculite and chlorite (0 to 5%), and pyrophyllite-talc association (0 to 8%). Sepiolite (12%) is only present in one paste. The grain size is made by variable proportions of sand (5 to 65%), silt (12 to 53%) and clayey fraction (18 to 66%). The Plasticity index (PI) indicates the presence of two principals groups of pastes. (1) The first group is characterized by PI values ranging between 15 to 18. Their moderate plasticity behavior is related either to the low rate of clayey fraction and/or to the absence of plastic clays like smectites and mixed layer with smectitic sheets. (2) The PI of second group ranges between 20 to 32. The high plasticity values are influenced by the presence of specific clayey minerals like talc-pyrophyllite or sepiolite, or by the high content of smectite plus smectitic mixed-layers within the clayey (< 2 micron) fraction. Only one sample without any of these plastic minerals but with a high content of clayey fraction is characterized by a high PI value (24). We concluded that the workability of ceramic paste in Marrakech region is controlled by a combination of factors, dominated by the grain size distribution and the content in plastic clay minerals within the fine fraction. [less ▲]

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See detailWhat are the determinants of the operational losses severity distribution ? A multivariate analysis based on a semiparametric approach.
Hambuckers, julien ULg; Heuchenne, Cédric ULg; Lopez, Olivier

Poster (2015, June)

In this paper, we analyse a database of around 41,000 operational losses from the European bank UniCredit. We investigate three kinds of covariates: firm-specific, fi- nancial and macroeconomic covariates ... [more ▼]

In this paper, we analyse a database of around 41,000 operational losses from the European bank UniCredit. We investigate three kinds of covariates: firm-specific, fi- nancial and macroeconomic covariates and we study their relationship with the shape parameter of the severity distribution. To do so, we introduce a semiparametric approach to estimate the shape parameter of the severity distribution, conditionally to large sets of covariates. Relying on a single index assumption to perform a dimension reduction, this approach avoids the curse of dimensionality of pure multivariate nonparametric techniques as well as too restrictive parametric assumptions. We show that taking into account variables measuring the economic well being of the bank could cause the required Operational Value-at-Risk to vary drastically. Especially, high pre-tax ROE, efficiency ratio and stock price are associated with a low shape parameter of the severity distribution, whereas a high market volatility, leverage ratio and unemployment rate are associated with higher tail risks. Finally, we discuss the fact that the considered approach could be an interesting tool to improve the estimation of the parameters in a Loss Distribution Approach and to offer an interesting methodology to study capital requirements variations throughout scenario analyses. [less ▲]

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See detailStudy of N-linked glycosylation in different life stages of the red flour beetle (Tribolium castaneum)
Smargiasso, Nicolas ULg; Walski, Tomasz; Van Damme, Els et al

Poster (2015, June)

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See detailReduced Order Model for Accounting for High Frequency Effects in Power Electronic Components
Paquay, Yannick ULg; Geuzaine, Christophe ULg; Hasan, Md. Rokibul et al

Poster (2015, June)

This paper proposes a reduced-order model of power electronic components based on the proper orthogonal decomposition. Starting from a full-wave finite-element model and several snapshots/frequencies, the ... [more ▼]

This paper proposes a reduced-order model of power electronic components based on the proper orthogonal decomposition. Starting from a full-wave finite-element model and several snapshots/frequencies, the reduced-order (RO) model is constructed. Local field values (e.g. magnetic flux density, electric current density, magnetic or electric field) and global quantities (e.g. characteristic complex impedance, joule losses) can be determined for the intermediate frequencies with a very low computational cost and high accuracy. Particular attention is paid to the choice of the most suitable snapshots by means of three different greedy algorithms, the performance of which is compared. We adopt an automatic greedy algorithm that depends only on the RO model. [less ▲]

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See detailExtracellular peptidase hunting for improvement of protein production in plant cells and roots
Lallemand, Jérôme ULg; Bouché, Frédéric ULg; Desiron, Carole et al

Poster (2015, June)

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant ... [more ▼]

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant proteins are now reaching the market, the production systems still need improvements to maximize their competitiveness, proteolysis being one of the main factors limiting the yields. Identifying and inhibiting in vivo endogenous proteases involved in the degradation of recombinant proteins could then lead to a significant increase in production yields. In this study, we focused on two different production systems in Arabidopsis thaliana: rhizosecretion and cell suspensions. Extracellular proteases of both systems were used in vitro to study the conditions of target protein degradation (Bovine Serum Albumine, BSA). First, proteases from both systems degrade BSA at both acidic and neutral-to-basic pH conditions. Then, serine and metallopeptidases were shown to be the main protease classes responsible for BSA degradation by rhizosecreted proteomes or extracellular cell culture media, respectively. Finally, the biochemical tests were coupled to a bioinformatics analysis of publicly available transcriptomic data, in order to reduce the number of the proteases most likely involved in BSA degradation. Using this method, only five serine proteases and two metallopeptidases remain candidates for an amiRNA-mediated in vivo inhibition. [less ▲]

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See detailEmpowering NGS technologies for the study and diagnostic of plant viruses - European COST Action FA1407
Massart, Sébastien ULg; Gentit, Pascal; Olmos, Antonio et al

Poster (2015, June)

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See detailNonlinear Interpolation on Manifold of Reduced Order Models in Magnetodynamic Problems
Paquay, Yannick ULg; Bruls, Olivier ULg; Geuzaine, Christophe ULg

Poster (2015, June)

Proper Orthogonal Decomposition (POD) is an efficient model order reduction technique for linear problems in computational sciences, recently gaining popularity in electromagnetics. However, its ... [more ▼]

Proper Orthogonal Decomposition (POD) is an efficient model order reduction technique for linear problems in computational sciences, recently gaining popularity in electromagnetics. However, its efficiency has been shown to considerably degrade for nonlinear problems. In this paper, we propose a reduced order model for nonlinear magnetodynamic problems by combining POD with an interpolation on manifolds, which interpolates the reduced bases to efficiently construct the desired solution. [less ▲]

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See detailCyber-pédopornographes : fixation pédophilique et Internet, une alternative à risque ?
Glowacz, Fabienne ULg; Galligani, Lucas; Hennen, Clarisse

Poster (2015, June)

La consommation de pédopornographie est un thème de recherche récent faisant suite à l’intérêt scientifique et clinique porté à la délinquance sexuelle. La question des rapports entre l’existence d’une ... [more ▼]

La consommation de pédopornographie est un thème de recherche récent faisant suite à l’intérêt scientifique et clinique porté à la délinquance sexuelle. La question des rapports entre l’existence d’une fixation pédophilique, la consommation de pédopornographie et les passages à l’acte pédophilique a été abordée par plusieurs études (Bourke et Hernandez, 2009 ; Babchishin, Hanson et Herman, 2010 ; Corriveau et Fortin, 2014) et reste en débat. Selon l’article 383bis du code pénal belge, la pédopornographie consiste en des « emblèmes, objets, films, photos, diapositives ou autres supports visuels présentant des mineurs d’âge dans des positions à caractère sexuel ». Cependant, le point de vue légal ne permet pas de prendre en considération tous les supports que peuvent utiliser les pédopornographes. En effet, certains amateurs trouveront satisfaction dans des contenus non-pornographiques. Dès lors, une typologie des images utilisées par les amateurs de pornographie enfantine a été mise en place par Taylor, Holland et Quayle (2001), sous le nom de COPINE Scale, reprenant dix catégories d’images pouvant être consultées ou téléchargées par les amateurs. Il existe différents lieux sur l’Internet sur lesquels ces supports se trouvent, le plus important étant les groupes de nouvelles, mais aussi plusieurs systèmes, comme que le peer-to-peer, dans lesquels les échanges de telles images s’opèrent. Plusieurs typologies ont été élaborées (Fortin et Roy, 2003 ; Delmonico et Griffin, 2008 ; Davidson, 2008 ; Corriveau et Fortin, 2011) identifiant divers profils : collectionneurs, distributeurs, abuseurs, producteurs et commerçants. Dans le cadre de notre recherche, à partir d’entrevues menées avec 9 sujets consommateurs de pédopornographie (âgés entre 29 et 60 ans) consultant au centre de santé mentale SYGMA, équipe de santé spécialisée, (8 d’entre eux dans le cadre d’une mesure judiciaire), nous avons exploré plusieurs dimensions pouvant être associées aux conduites de consommation de pédopornographie : l’addiction à internet, la présence d’une fixation pédophilique, la tendance à l’ennui, l’impulsivité et la recherche de sensation, la présence d’un soutien social ainsi que le rôle de sous-cultures pédopornograhiques. Au niveau méthodologique, nous avons adopté une approche mixte basée sur un entretien semi-structuré et la passation de tests (Echelle de provision sociale, Caron, 1996 ; Echelle de disposition à l’ennui, Gana et Akrmi, 1988 ; Implusive Behavior Scale, Whiteside et Lynam, 2001 ; Mesure de l’intensité des conduites addictives appliquée à l’internet, Décamps, Battaglia, et Idier, 2010) et sur une analyse du dossier clinique comprenant les jugements, les avis motivés et les entretiens cliniques. Nos résultats indiquent que la fixation pédophilique pouvait être un moteur de la consommation sans pour autant être présente chez tous les sujets. La tendance à l’ennui est une dimension peu signifiante dans la dynamique de consommation. Ce sont le manque de soutien social et l’insatisfaction affective et sexuelle qui se retrouvent au centre des dynamiques de la consommation pédopornographique de nos sujets. Isolés socialement et présentant un développement problématique sur le plan sexuel et affectif, c’est notamment par la consommation de pédopornographie que les sujets disent avoir trouvé une alternative. Cette consommation renvoie par ailleurs à un manque de contrôle social, offrant l’opportunité de consommer, ainsi qu’une impossibilité d’identifier un partenaire sexuel. L’Internet sera alors vu comme un outil permettant de répondre à ces difficultés sexuelles, sans pour autant qu’il n’y ait de comportement addictif. [less ▲]

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See detailFunctional analysis of FRD3 in Arabidopsis
Scheepers, Maxime ULg; Charlier, Jean-Benoit ULg; Spielmann, Julien ULg et al

Poster (2015, June)

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. The FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of ... [more ▼]

Zinc and iron are two essential micronutrients for plants. The homeostasis networks of the two metals are intertwined. The FRD3 (FERRIC REDUCTASE DEFECTIVE 3) protein, a member of the MATE family of membrane transporters, is a citrate transporter involved in iron homeostasis and playing a role in zinc tolerance in Arabidopsis. The FRD3 gene displays a complex regulation. Alternative transcript initiation for FRD3 determines two transcripts, which differ in their 5'UTRs and have differential translation efficiency. The two transcripts are selectively regulated under stress conditions: iron and zinc depletion, zinc excess or cadmium presence. We are aiming to determine the FRD3 function in zinc and iron homeostasis in Arabidopsis. We will present data (i) on the functional characterization of the alternative transcripts and their role in metal homeostasis in Arabidopsis and (ii) on the zinc phenotypes of the frd3 mutant. [less ▲]

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See detailUnravelling Cemip expression and functions in the auditory portion of the inner ear.
Czajkowski, Amandine ULg; Chariot, Alain ULg; Delacroix, Laurence ULg et al

Poster (2015, May 29)

The inner ear is a complex organ composed of the vestibular system – which is the balancing system – and the cochlea – which is the earing system. The cochlea is a coiled shape organ composed of three ... [more ▼]

The inner ear is a complex organ composed of the vestibular system – which is the balancing system – and the cochlea – which is the earing system. The cochlea is a coiled shape organ composed of three main structures: the spiral ligament sitting on top of the stria vascularis, the organ of Corti with sensory hair cells and supporting cells and the spiral ganglion composed of neurons and glial cells. After an auditory stimulus, the sound wave progresses in the scala media filled with endolymph and induces a stimulation of sensory hair cells. These cells then transmit the information to the spiral ganglion neurons connected to them. Of course, the correct ionic homeostasis of endolymph is required for a good sound wave transmission. This homeostatic function is assured by the stria vascularis and the spiral ligament. The alteration of one of the structures mentioned before induces deafness. Currently, numerous genes have been associated to this kind of hearing loss. In the present work, we focus our attention Cemip – also known as KIAA1199 – that has been associated to human hereditary neurosensory deafness. Indeed, three missense mutations consisting in non-synonymous amino acid changes (R187L, R187H and H783Y) have been associated to this form of deafness. Therefore we would like to understand the role of Cemip in the cochlea. For that we have analysed Cemip mRNA pattern of expression by in situ hybridization at different developmental stages on cochlear sections. It seems Cemip mRNA is not present in the auditory portion of the inner ear at early embryonic stage 14 (E14) while it is largely present at E17 in the spiral ganglion, in supporting cells of the organ of Corti and in the spiral ligament. This expression is maintained post-nattily until P7. At P21 the expression is restricted to the spiral lamina - an osseous structure surrounding the spiral ganglion. Our on going work is aimed at revealing the biological role of Cemip in the cochlea in conditional knock-out mice. [less ▲]

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See detailWork incapacity and chronic pain patients: Is there an impact of work incapacity on anxiety and depression?
Beaumel, Alexia; Vanootighem, Valentine ULg; Gillet, Aline ULg et al

Poster (2015, May 28)

In 2011, a report of the Federal Public Service have shown that 8.5% of the Belgian population suffers from chronic pains, which represents 938 300 belgian people (Berquin et al., 2011). These persistent ... [more ▼]

In 2011, a report of the Federal Public Service have shown that 8.5% of the Belgian population suffers from chronic pains, which represents 938 300 belgian people (Berquin et al., 2011). These persistent pains may result in functional limitations on both domestic and professional domains which may lead to temporary or permanently work incapacity (Faymonville et al., 2014). These incapacities may in turn cause a social withdrawal and a tendency to focus on pain (Berquin et al., 2011). Additionally, patients are prone to emotional modifications due to persistent pain (Ossipov et al., 2010). All of these factors may contribute in the emergence of anxiety and depressive symptoms. The present study examines the impact of type of work incapacities (at work, temporary incapacity and permanent incapacity) on anxiety and depression scores as a function of diagnoses (fibromyalgia, chronic pain syndrome, back pain, polyalgia). 123 patients suffering from chronic pain completed a measure of anxiety and depression (HADS), and a self-reported questionnaire of psychological and physical disability at work. We did not found any significant effect of type of work incapacities on anxiety and depression scores. Results present tables of frequencies to illustrate the social and professional situations of patients as a function of chronic pain diagnoses. [less ▲]

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See detailExecutive functions in normal aging: Impact of cognitive reserve and objective physical activity
Gilsoul, Jessica ULg; Simon, Jessica ULg; Collette, Fabienne ULg

Poster (2015, May 28)

It is now acknowledged that there exists a large inter-individual variability in age-related cognitive changes. According to Stern (2009), the cognitive reserve built up throughout the life span will make ... [more ▼]

It is now acknowledged that there exists a large inter-individual variability in age-related cognitive changes. According to Stern (2009), the cognitive reserve built up throughout the life span will make seniors more or less resilient to the deleterious effects of aging on cognition, with seniors having a high level of cognitive reserve being more prone to successfully cope with cognitive and neuronal changes. Currently, few studies have focused on the specific effects of different factors of cognitive reserve on cognition. Consequently, the objective of this study is to determine the impact of four determinants of cognitive reserve on executive functions in normal aging. Sixty-eight healthy participants aged 60 to 80 were enrolled. Regarding executive functions, Inhibition (Stroop test, Hayling test, TAP Incompatibility subtest), Shifting (TAP Flexibility subtest, Plus-Minus task), and Updating (Letter-Number Sequencing subtest from MEM III, Letter memory task, 2-back task) were assessed (Miyake et al., 2000). Cognitive reserve was measured by questionnaires assessing educational level, occupation, leisure activities and physical activity. Moreover, an accelerometer allowed quantitative measure of physical activity by recording body movements during two weeks. Group comparisons (p<0.05) showed that seniors with a high level of cognitive reserve (measured by a global composite z-score) have better updating abilities (Letter memory task and updating composite score). In order to test the influence of each cognitive reserve factor on executive functions, simple linear regressions (p<0.05) were performed. The results showed that the educational level accounts for a significant part of the variance in flexibility (TAP Flexibility) and updating (Letter-Number sequencing, 2-back and composite score). The occupational level accounts significantly for the variance of the updating composite score. Regarding the quantitative physical activity, the mean number of minutes per day in moderate or intense physical activity significantly predicted the updating composite score. These results indicate that the level of cognitive reserve is related to abilities in some executive tests, particularly updating abilities. Importantly, all aspects of cognitive reserve are not associated with the three executive processes. This indicates a specificity of the protective effect of cognitive reserve on executive abilities. [less ▲]

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See detailRomantic relationship and delinquency : which desistance factor ?
Puglia, Rosa ULg; Lepas, Jennifer; Glowacz, Fabienne ULg

Poster (2015, May 28)

A large number of studies (Stouthamer-Loeber, Loeber, Wei, Farrington & Wikstrom, 2002) have shown that the quality of parent-child relationship influences delinquency behavior during adolescence. So, the ... [more ▼]

A large number of studies (Stouthamer-Loeber, Loeber, Wei, Farrington & Wikstrom, 2002) have shown that the quality of parent-child relationship influences delinquency behavior during adolescence. So, the poorer the quality of relationship between a parent and his child is, the higher the probability that the child adopts delinquent behavior. However, recent studies (Giordano et al., 2002 ; Laub et al., 1998 ; Meeus & al., 2004 ; Woordward et al., 2002) have questioned the persistence of this association when people involved in a romantic relationship. These research have shown that quality of parent-child relationship predict delinquency in adolescents and young adults who do not have an intimate partner. But in adolescents and young adults who have an intimate partner, quality of parent-child relationship becomes obsolete. To be involved in an intimate relationship leads to less delinquency. Furthermore, results are concordant with research about desistance from delinquency that have shown that marriage leads to less criminality (Forrest & Hay, 2011 ; Sampson & Laub, 1993). These studies (Meus & al., 2004 ; Sampson & Laub, 1993) specify that it is quality of relationship rather than simply being in a romantic relationship that have an impact on desistance from delinquency. From empirical research conducted with adolescents and young adults (N=283) aged from 16 to 22 years old, and from a clinical research, we have explored links between parental attachment, romantic attachment and delinquency. We wondered if romantic attachment promotes desistance from delinquency whatever parental attachment. Behavioral System Questionnaire (Wehner & Furman, 1999) and self-reported delinquency scale (Born & Gavray, 1994) have used in this context. It appears that most of the participants have been involved in a significant romantic relationship. This romantic relationship is mainly characterized by a secure attachment. So, it leads to less deviant behaviors. On the other hand, parental attachment does not lead to less deviant behaviors. It also appears that attachment style that one develops towards one’s parent is not unchangeable. Romantic relationship during adolescence and adulthood can bring emotional and relational modifications. [less ▲]

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See detailPsychopathy and emotions: would emotional distancing make more efficient in cognitive tasks?
Grandjean, Sylvie; Dehon, Hedwige ULg; Blavier, Adelaïde ULg et al

Poster (2015, May 28)

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See detailBorderline Personality Disorder through the Rorschach test
Rommes, Jennifer; Englebert, Jérôme ULg; Blavier, Adelaïde ULg

Poster (2015, May 28)

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See detailThe effect of ageing and encoding instructions on episodic memory
Hagelstein, Catherine ULg; François, Sarah ULg; Manard, Marine ULg et al

Poster (2015, May 28)

During ageing, a decline in episodic memory is observed, characterized by decreased recollection (a). In this study, we investigated the influence of encoding instructions, intentional or incidental, on ... [more ▼]

During ageing, a decline in episodic memory is observed, characterized by decreased recollection (a). In this study, we investigated the influence of encoding instructions, intentional or incidental, on the performance of younger and older adults during an episodic memory task. Twenty young volunteers (aged 18 to 30 years old) and 20 older volunteers (aged 61 to 72 years old) participated in this experiment. The stimuli consisted of 300 black-and-white drawings of common objects. The task comprised two steps. During the encoding phase, 100 items were presented once ("hard" condition) and 100 other items were presented twice ("easy" condition). During recognition, the items from the encoding phase were presented again, as well as 100 new items. In each age group, half of the participants received incidental encoding instructions (they had to make size judgements about the objects depicted) while the other half were explicitly asked to memorize the objects presented because they would be asked to recognize them later on (intentional encoding). During recognition, they performed a Remember-Know judgement for the items they believed they had seen earlier. We carried out ANOVAs in order to test for the influence of instructions, age group and number of repetitions of the items in one hand on the percentage of recollection and on the other hand on the percentage of familiarity (p<.05). Results show a significant effect of age, with more correct recollection responses in young adults, whereas more correct familiarity was found in older adults. Furthermore, it was found that the items presented twice lead to more recollection than those presented only once. Finally, these results also suggest that in the older group of participants, only for the items presented twice, intentional encoding instructions lead to more recollection and less familiarity. This finding is consistent with previous work showing that the elderly do not easily use elaborate encoding strategies, and that they need more support (here, a second exposition to the material) in order to perform a deep encoding when they are encouraged to do so with intentional learning instructions (b). (a) Bugaiska, A., Clarys, D., Jarry, C., Taconnat, L., Tapia, G., Vanneste, S., & Isingrini, M. (2007). The effect of aging in recollective experience: the processing speed and executive functioning hypothesis. Consciousness and Cognition, 16(4), 797-808. doi: 10.1016/j.concog.2006.11.007 (b) Froger, C., Bouazzaoui, B., Isingrini, M., & Taconnat, L. (2012). Study time allocation deficit of older adults: the role of environmental support at encoding? Psychology and Aging, 27(3), 577-588. doi:10.1037/a0026358 [less ▲]

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See detailMaternal representations, social context and substance abuse
Halin, Stéphanie ULg; Combe, Samantha; Glowacz, Fabienne ULg et al

Poster (2015, May 28)

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See detailThe impact of ageing on episodic memory encoding: an fMRI study
François, Sarah ULg; Angel, Lucie; Salmon, Eric ULg et al

Poster (2015, May 28)

It is now commonly accepted that a decline in episodic memory is observed with ageing: while recollection processes are impaired, familiarity seems to be relatively preserved (a). Older individuals appear ... [more ▼]

It is now commonly accepted that a decline in episodic memory is observed with ageing: while recollection processes are impaired, familiarity seems to be relatively preserved (a). Older individuals appear to recruit prefrontal areas bilaterally when their encoding is successful, while in young adults this activation is found to be left-lateralized (b). In this study, we were interested in the differences between younger and older participant regarding cerebral activity during encoding depending on whether the item elicited recollection or familiarity during the recognition phase. Twenty young volunteers (aged 19 to 29 years old) and 19 older volunteers (aged 60 to 78 years old) were presented visual stimuli depicting objects. During a first fMRI session, they were asked to make a size judgement about them. Then, in a second phase, the subjects were shown the items previously encountered during the encoding phase, as well as distractors. Participants' task was to determine which one were new and which one were seen earlier. For the latter, they also performed a Remember-Know judgement. Data were analysed using SPM8, with an event design comparing modifications in cerebral activity between the two subjects groups during encoding (1) for the items leading to recollection during the recognition phase compared to those leading to familiarity, and (2) for the items associated with familiarity during the recognition phase compared to those which were not recognized. Results show that older adults display a heightened activity in the right middle frontal gyrus, medial cingulate and paracingulate left gyri as well as in the precuneus, bilaterally when they engage recollection processes. Given that activations in these areas did not correlate with performance, they could be interepreted either as dedifferentiation or as an attempt of the ageing brain to compensate for a less elaborate encoding. However, no increase of activity was associated with familiarity processes in older adults, possibly because they are less demanding regarding attentional resources. (a) Bugaiska, A., Clarys, D., Jarry, C., Taconnat, L., Tapia, G., Vanneste, S., & Isingrini, M. (2007). The effect of aging in recollective experience: the processing speed and executive functioning hypothesis. Consciousness and Cognition, 16(4), 797-808. doi: 10.1016/j.concog.2006.11.007 (b) Duverne, S., Motamedinia, S., & Rugg, M. D. (2009). The relationship between aging, performance, and the neural correlates of successful memory encoding. Cerebral Cortex, 19(3), 733-744. doi:10.1093/cercor/bhn122 [less ▲]

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See detailThe neural bases of proactive and reactive control processes in healthy aging
Manard, Marine ULg; François, Sarah ULg; SALMON, Eric ULg et al

Poster (2015, May 28)

Background. Research on cognitive control suggests an age-related decline in proactive control abilities (an anticipatory form of control) whereas reactive control (consecutive to conflict detection ... [more ▼]

Background. Research on cognitive control suggests an age-related decline in proactive control abilities (an anticipatory form of control) whereas reactive control (consecutive to conflict detection) seems to remain intact [1]. As proactive and reactive control abilities were associated to specific brain networks [2], this study investigated age-related effects on the neural substrates associated to each kind of control. Method. A modified form of the Stroop task was administered to 16 young and 16 older adults in an event-related fMRI experiment. In this version of the Stroop task, three different contexts were created: (1) a mostly congruent context (MC, inducing reactive control) with a majority of congruent items, (2) a mostly incongruent context (MI, inducing proactive control) with mainly interfering items, (3) a neutral context (MN) with mainly neutral items. Preprocessing and statistical analyses were performed with SPM8 (p<.001 uncorrected). Results. Behavioral results (p<0.05) indicated faster processing of interferent items in the MI than MC context in young participants only. With regard to neuroimaging data, the comparison of the two groups showed that the processing of interferent items in the MI context is associated to decreased activity in (mainly right-sided) frontal and temporal areas in the older group. On the contrary, in the MC context, increased activity was observed in bilateral frontal areas for older compared to younger participants. Discussion. Behavioral results confirm that older participants have difficulties with the implementation of proactive control that is associated to decreased brain activity (compared to young participants) in areas underlying Stroop performance. However, the recruitment of supplementary frontal areas we observed in the reactive control condition suggests compensation processes. So, aging seems to differentially affect the neural networks associated to the various kinds of cognitive control. [less ▲]

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See detailParental and romantic attachments in teen datings : different meanings in boys' and girls' intimacy
Courtain, Audrey ULg; Lepas, Jennifer; Boulard, Aurore ULg et al

Poster (2015, May 28)

First proposing a relational understanding of the child’s very first years, attachment theory has since then been taken into consideration in friendships and romantic relationships. Indeed, the internal ... [more ▼]

First proposing a relational understanding of the child’s very first years, attachment theory has since then been taken into consideration in friendships and romantic relationships. Indeed, the internal working models (IWM) built upon early interactions are to be used on and on in many other situations as guidelines to understand what is going on for the self, this particular other and their specific relationship. Different attachment styles are thus likely to develop: secure or insecure (anxious/preoccupied, avoidant/dismissive, fearful). Considering teen dating, attachment is deeply involved, especially for two reasons: first, a certain questioning, but also re-actualization, of the attachment to parents; second, a new kind of attachment, meaning the one to the romantic partner. These elements lead us to test hypotheses linking attachment to parents and attachment to romantic partners, but also linking these attachments to some teen dating characteristics, such as the age when first dating, the number of love relationships, the longest-lasting love relationship and the fear of intimacy. Our hypothesis was tested among 283 teenagers (mean age = 17.43) with two self-administered questionnaires (the Behavioral Systems Questionnaire and the Fear of Intimacy Scale). Our statistical analyses were made of Pearson Chi², Anova and Post-Hoc Fisher (LSD). We see three types of interesting results. First, there is a significant relationship between attachment (parental and romantic attachments) and the number of love relationships and the fear of intimacy. Second, these relationships are differently established once considered the attachment modalities. Third, gender differences appear, girls looking more influenced by their attachment style than boys. [less ▲]

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See detailElectron paramagnetic resonance and fluorescence studies on potential anticancer properties of two new Ru(II) complexes : preliminary results
Collienne, Simon ULg; Terrak, Mohammed ULg; Mouithys-Mickalad, Ange ULg et al

Poster (2015, May 22)

Fight against cancer is a priority of today’s research. Since the discovery of the anticancer properties of cisplatin (CisPt) in 1965 by Rosenberg [1], the treatment of cancer by chemotherapy has known ... [more ▼]

Fight against cancer is a priority of today’s research. Since the discovery of the anticancer properties of cisplatin (CisPt) in 1965 by Rosenberg [1], the treatment of cancer by chemotherapy has known great improvements. Unfortunately, CisPt has several side effects and is not effective against all kinds of cancer. Nevertheless its use highlights the great potential of organometallic compounds in the treatment of cancer [2]. Here we investigated the potential anticancer properties of two new organometallic compounds based on ruthenium II : [RuCl(p-cymene)(S2C.IDip)]+(PF6)- and [RuCl(p-cymene)(S2C.ICy)]+(PF6)-, named as LDO436 and LDO437 respectively. [less ▲]

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See detail"Tell me about Self." Clarté de soi et souvenirs définissant le soi: Particularités du self chez des individus souffrant d'un trouble de l'humeur
Boulanger, Marie ULg; Wagener, Aurélie ULg; Blairy, Sylvie ULg

Poster (2015, May 21)

The present study focusses on a particular kind of autobiographical memories called self-defining memories (SDMs). SDMs are highly pertinent for self-building because they help to define in the most ... [more ▼]

The present study focusses on a particular kind of autobiographical memories called self-defining memories (SDMs). SDMs are highly pertinent for self-building because they help to define in the most clearly way our perception of ourselves. If literature showed that depressed and bipolar patients suffer from autobiographical memory (AM) disturbances, SDMs have not been studied a lot since yet in these two disorders. Then, the present study pursues two main aims: (1) to assess SDM’s characteristics and (2) to assess the relationship between the ability to retrieve SDM’s and self-concept clarity. To reach our objective, 17 depressed and 16 bipolar patients are compared to 17 healthy subjects. Participants are asked to complete SDMs procedure that is recalling six important events which happened in their life. These SDMs are assessed on four variables: specificity, impact, valence, meaning making, contain. Self-concept clarity and mood are also assessed. Preliminary results do not highlight difference between our groups on specificity. Marginal effects appear for meaning making with healthy subjects recalling more meaning making events. Results show that depressed patients present a mood congruence. The final results will be presented during the convention, as data-collection is on-going and will end in April. Experimental and clinical implications of our results will be discussed. [less ▲]

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See detailAgroecology: Unity into diversity
Hatt, Séverin ULg; Artru, Sidonie ULg; Boeraeve, Fanny ULg et al

Poster (2015, May 21)

What does agroecology suggest ? Next to the theoretical agroecological principles, we present illustrative examples from farming practices, through the food system, up to the way of carrying agricultural ... [more ▼]

What does agroecology suggest ? Next to the theoretical agroecological principles, we present illustrative examples from farming practices, through the food system, up to the way of carrying agricultural research and education. [less ▲]

Detailed reference viewed: 23 (2 ULg)