Le bioraffinage, une alternative prometteuse à la pétrochimieLaurent, Pascal ; Roiz, Julie ; Wertz, Jean-Luc et alin Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(4), 597-610 Because of the price increase of fossil resources, of their uncertain availability and because of environmental concerns, alternative solutions able to mitigate global warming, and reduce the consumption ... [more ▼] Because of the price increase of fossil resources, of their uncertain availability and because of environmental concerns, alternative solutions able to mitigate global warming, and reduce the consumption of fossil fuels and carbon dioxide emissions should be promoted. The replacement of petroleum with biomass as raw material for bioenergy (biofuels, power and heat) and chemical production is an interesting option and is the driving force for the development of biorefinery complexes that will have a critical role to play in our common future. A biorefinery is a facility that integrates biomass conversion processes and equipment to produce fuels, power, heat and chemicals from biomass. In biorefinery, almost all types of biomass feedstocks can be converted to different classes of biofuels and biochemicals through various processes that maximize economic and environmental benefits, while minimizing waste and pollution. Through the integration of green chemistry into biorefineries, and the use of low environmental impact technologies, future sustainable production chains of biofuels and high value chemicals from biomass can therefore be established. Currently, the green biorefinery, the whole-crop biorefinery, the oilseed biorefinery and the lignocellulosic feedstock biorefinery are favoured in research, development and industrial implementation, essentially through fully integrated biorefinery complexes. [less ▲] Detailed reference viewed: 44 (19 ULg) Bioraffinerie végétale : chimie et technologie des structures osidiques (TECHNOSE); Paquot, Michel ; Wathelet, Jean-Paul et alReport (2012) Detailed reference viewed: 3 (0 ULg) Bioreactor hydrodynamic effect on Escherichia coli physiology: experimental results and stochastic simulations.Delvigne, Frank ; Destain, Jacqueline ; Thonart, Philippe ![]() in Bioprocess and Biosystems Engineering (2005), 28(2), 131-7 A microorganism circulating in a bioreactor can be submitted to hydrodynamic conditions inducing a significant effect on its physiology. The mixing time exhibited by the stirred bioreactor and the ... [more ▼] A microorganism circulating in a bioreactor can be submitted to hydrodynamic conditions inducing a significant effect on its physiology. The mixing time exhibited by the stirred bioreactor and the circulation of microorganisms are both involved in this reacting system. The mixing component determines the intensity of the concentration gradient and the circulation component determines the way in which the microorganism is exposed to this gradient. These two components linked to the experimental evaluation of microbial physiology can be analysed by a structured stochastic model in the case of a partitioned or "scale-down" reactor (SDR). A stochastic model indeed enables to simulate the mixing process as well as the circulation of microorganisms in SDRs. The superimposition of mixing and circulation processes determines the concentration profile experienced by a microorganism in the reactor. In the present case, the glucose concentration experienced by Escherichia coli has been modelled during a fed-batch culture. In this context, the use of a stochastic hydrodynamic model has permitted to point out an interesting feed pulse retardant effect in the SDRs. Nevertheless, the metabolic response of E. coli is not easy to interpret because of the possible simultaneous developments of overflow metabolism and mixed acid fermentation induced by the strong glucose concentration in the reactor. [less ▲] Detailed reference viewed: 32 (2 ULg) Bioreactor mixing efficiency modulates the activity of a prpoS::gfp reporter gene in E. coliDelvigne, Frank ; Boxus, Mathieu ; et alin Microbial Cell Factories (2009), 8(15), ABSTRACT: BACKGROUND: Extensive studies have shown that up-scaling of bioprocesses has a significant impact on the physiology of the microorganisms. Among the factors associated with the fluid dynamics of ... [more ▼] ABSTRACT: BACKGROUND: Extensive studies have shown that up-scaling of bioprocesses has a significant impact on the physiology of the microorganisms. Among the factors associated with the fluid dynamics of the bioreactor, concentration gradients induced by loss of the global mixing efficiency associated with the increasing scale is the main phenomena leading to strong physiological modifications at the level of the microbial population. These changes are not fully understood since they involve complex physiological mechanisms. In this work, we intend to investigate, at the single cell level, the expression of the rpoS gene associated with the stress response of E. coli. The cultures of the reporter strain have been performed in a small scale reactor as well as in a series of scaled-down bioreactors able to induce extracellular perturbations with increasing level of magnitude. RESULTS: The rpoS level has been monitored by the aim of a transcriptional reporter gene based on the synthesis of the green fluorescent protein (GFP). It has been observed that the level of GFP increases during the transition from batch to fed-batch phase. After this initial increase, the GFP content of the cell drops, primarily due to the dilution by cell division. However, a significant drop of the GFP content has been observed if using a partitioned bioreactor, for which the mixing conditions are very bad, leading to the exposure of the cells to cyclic and stochastic extracellular fluctuations. If considering the flow cytometric profile of the cell to cell GFP content, this drop has to be attributed to the appearance of segregation at the level of the GFP content among the microbial population. CONCLUSION: The generation of extracellular perturbations (in the present case, at the level of the sugar concentration and the dissolved oxygen level) has led to a drop at the level of the rpoS expression level. This drop has to be attributed to a segregation phenomenon in microbial population, with a major sub-population exhibiting a low expression level and a minor sub-population keeping its initial elevated expression level. The intensity of the segregation, as well as its time of appearance during the culture can be related to the bioreactor mixing efficiency. [less ▲] Detailed reference viewed: 45 (18 ULg) BIOREMEDIATION DE SOLS POLLUES AUX HYDROCARBURES – AMELIORATION DE LA BIOREMEDIATION IN SITU DES HYDROCARBURES PAR BIO-AUGMENTATION AU MOYEN DE SOUCHES ENDOGENES ET EXOGENESTarayre, Cédric ![]() Master's dissertation (2010) This study relates to biological treatment of hydrocarbon polluted soils. Its purpose is to establish an industrial approach of bioaugmentation and to verify its efficiency compared with biostimulation ... [more ▼] This study relates to biological treatment of hydrocarbon polluted soils. Its purpose is to establish an industrial approach of bioaugmentation and to verify its efficiency compared with biostimulation. The purpose is to simulate in situ bioremediation using experimental columns. It has been demonstrated that it’s possible to produce a biomass able to degrade hydrocarbons in a polluted soil in one month. The main way of hydrocarbon elimination in the soil is biodegradation and the amount of hydrocarbons removed by water is negligible (1 % in each case). The addition of exogenous strains into the soil leads to the best biodegradation rate (39,3 % in 63 days). An endogenous bioaugmentation was tried but the efficiency was not demonstrated. On the other hand, the use of an artificial consortium was not more satisfying. Biostimulation alone led to a biodegradation rate of 23,9 % in 63 days. The lack of oxygen was avoided and evaluated through nitrite and ammonium concentrations in the water. These concentrations were negligible and always less than 2,5 and 1 mg/l, respectively. This work also tried to develop a special technique of monitoring of microbial ecology with microplates (Biolog ECO Micro PlateTM). The results were analysed through two different statistical methods : similarity rates and principal component analysis. It has been demonstrated that the column which leads to the best biodegradation rate also shows a particular metabolic profile, according to principal component analysis. The similarity rate is only 31 % with the initial microflora. This microflora seems to change during the experience because the similarity rate between the initial and the final microfloras is only 61 %. This work proved the efficiency of exogenous bioaugmentation compared with biostimulation. It proposes to apply this technique on a large scale, but only if the amount of polluted soil is evaluated before. [less ▲] Detailed reference viewed: 204 (22 ULg) Bioremediation of diesel-polluted soils.; ; et al Poster (1995, February) Detailed reference viewed: 4 (0 ULg) Bioremediation of diesel-polluted soils.; ; et al in Archives Internationales de Physiologie et de Biochimie (1995), 103(B46), Detailed reference viewed: 1 (1 ULg) Bioremediation of hydrocarbon polluted soil - Improvement of in situ bioremediation by bioaugmentation with endogenous and exogenous strainsTarayre, Cédric ![]() Poster (2010, September 03) Petroleum pollution has now become a real problem because hydrocarbons are persistent contaminants in soils and water. Contamination problems increase when ages of relevant facilities, such as oil storage ... [more ▼] Petroleum pollution has now become a real problem because hydrocarbons are persistent contaminants in soils and water. Contamination problems increase when ages of relevant facilities, such as oil storage tanks and pipelines, increase over time. The evolution of Legislation concerning soil pollution has led to the need of efficient techniques able to restore the polluted ground. Unfortunately, these techniques are expensive. Bioremediation of hydrocarbon polluted soils has been recognized as an efficient, economic and environmentally sound treatment. Particularly biostimulation and bioaugmentation are being studied in labs. A lot of studies tried to determine which method is the most efficient, but the conclusions diverge. Moreover, conditions are different in labs and contaminated sites. [less ▲] Detailed reference viewed: 14 (10 ULg) Bioresorbable and bioactive composite materials based on polylactide foams filled with and coated by Bioglass (R) particles for tissue engineering applications; ; et al in Journal of Materials Science: Materials in Medicine (2003), 14(5), 443-450 Poly(DL-lactide) (PDLLA) foams and bioactive glass (Bioglass®) particles were used to form bioresorbable and bioactive composite scaffolds for applications in bone tissue engineering. A thermally induced ... [more ▼] Poly(DL-lactide) (PDLLA) foams and bioactive glass (Bioglass®) particles were used to form bioresorbable and bioactive composite scaffolds for applications in bone tissue engineering. A thermally induced phase separation process was applied to prepare highly porous PDLLA foams filled with 10wt% Bioglass® particles. Stable and homogeneous layers of Bioglass® particles on the surface of the PDLLA/Bioglass® composite foams as well as infiltration of Bioglass® particles throughout the porous network were achieved using a slurry-dipping technique. The quality of the bioactive glass coatings was reproducible in terms of thickness and microstructure. In vitro studies in simulated body fluid (SBF) were performed to study the formation of hydroxyapatite (HA) on the surface of the PDLLA/Bioglass® composites, as an indication of the bioactivity of the materials. Formation of the HA layer after immersion in SBF was confirmed by X-ray diffraction and Raman spectroscopy measurements. The rate of HA formation in Bioglass®-coated samples was higher than that observed in non-coated samples. SEM analysis showed that the HA layer thickness rapidly increased with increasing time in SBF in the Bioglass®-coated samples. The high bioactivity of the developed composites suggests that the materials are attractive for use as bioactive, resorbable scaffolds in bone tissue engineering. [less ▲] Detailed reference viewed: 43 (3 ULg) Biorthogonal wavelets in H-m(R)Bastin, Françoise ; in Journal Of Fourier Analysis And Applications (1998), 4(6), 749-768 This article is concerned with constructions of biorthogonal basis of compactly supported wavelets in Sobolev spaces of integer order. Using techniques of [1] and [2], the results presented here ... [more ▼] This article is concerned with constructions of biorthogonal basis of compactly supported wavelets in Sobolev spaces of integer order. Using techniques of [1] and [2], the results presented here generalize to Sobolev spaces some constructions of Cohen et al. [7] and Chui and Wang [5] established in L-2(R). [less ▲] Detailed reference viewed: 13 (3 ULg) Biorthogonalization Techniques for Least Squares Temporal Difference LearningJung, Tobias ; Ernst, Damien ![]() Poster (2012, December 07) We consider Markov reward processes and study OLS-LSTD, a framework for selecting basis functions from a set of candidates to obtain a sparse representation of the value function in the context of least ... [more ▼] We consider Markov reward processes and study OLS-LSTD, a framework for selecting basis functions from a set of candidates to obtain a sparse representation of the value function in the context of least squares temporal difference learning. To support efficient both updating and downdating operations, OLS-LSTD uses a biorthogonal representation for the selected basis vectors. Empirical comparisons with the recently proposed MP and LARS frameworks for LSTD are made. [less ▲] Detailed reference viewed: 33 (11 ULg) Biosafety of Herpesvirus Vectors; ; et al in Current Gene Therapy (2003), 3(6), 597-611 Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in ... [more ▼] Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in which the viral genome exists as a stable episome in the nucleus. For gene therapy to become a potential future treatment option, biosafe therapeutically efficient gene transfer is a central, but more and more stringent requirement. This review highlights the progress in development of herpesvirus based vectors, describes their properties as wall as discusses the biosafety concerns that are associated with their use in gene therapy. Thought was also given to biosafety issues pertaining to design and production of herpesvirus vector systems in therapeutic gene delivery. [less ▲] Detailed reference viewed: 12 (1 ULg) Biosecurity in my Equine PracticeVan Galen, Gaby ![]() in Denis Verwilghen; Gaby van Galen; Valeria Busoni (Eds.) proceedings of Hippos 2012 (2012, February 12) Detailed reference viewed: 11 (3 ULg) Biosensors based on electrochemically prepared polyanilines; Jérôme, Robert ; Jérôme, Christine ![]() Poster (2005, June 01) Detailed reference viewed: 15 (3 ULg) Biosensors based on electrochemically prepared polyanilines and bifunctional hybrid proteinsFaure, Emilie ; ; Ruth, Nadia et alPoster (2007, August 31) Detailed reference viewed: 29 (11 ULg) Biosensors in Forensic SciencesFrederickx, Christine ; Verheggen, François ; Haubruge, Eric ![]() in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(3), 449-458 A biosensor is a device that uses biological materials to monitor the presence of specific chemicals in an area. Traditional methods of volatile detection used by the law enforcement agencies and rescue ... [more ▼] A biosensor is a device that uses biological materials to monitor the presence of specific chemicals in an area. Traditional methods of volatile detection used by the law enforcement agencies and rescue teams consist in the canine olfaction. This concept of using dogs to detect specific substances is quite old. However, dogs have some limitations such as cost of training, time of conditioning. Thus, the possibility of using other organisms as biosensors including rats, dolphins, honeybees, parasitic wasps in detection of explosives, narcotics and cadavers has been developed. But, insects have several advantages unshared by mammalians. Insects are very sensitive, cheap to produce and can be conditioned with impressive speed for a specific chemical-detection task. Moreover, insects might be a preferred sensing method in scenarios that are deemed too dangerous to use mammalian. The purpose of this review is to provide an overview of the biosensors used in forensic sciences [less ▲] Detailed reference viewed: 151 (15 ULg) Biosensors in Forensic SciencesFrederickx, Christine ; Verheggen, François ; Haubruge, Eric ![]() Conference (2010, May 05) Detailed reference viewed: 37 (3 ULg) Biosignatures morphologiques: quelques pistes de réflexionJavaux, Emmanuelle ![]() Conference (2006) Detailed reference viewed: 6 (0 ULg) Biosolubilization of copper from waste electric cablesGaydardzhiev, Stoyan ; Bastin, David ; Goffinet, Florence et alin Harre, Jens (Ed.) Proceedings of the Copper 2010 International Conference (2010, June) The paper reports on results from a laboratory tests for bacterial leaching of copper from scrap cables. The studied material is a reject fraction obtained after dismantling and separation of electric ... [more ▼] The paper reports on results from a laboratory tests for bacterial leaching of copper from scrap cables. The studied material is a reject fraction obtained after dismantling and separation of electric cables during recycling of end-of-life vehicles (ELV). The copper has been met predominately in pure metallic form as tiny irregular shaped wires often coated with tin and well liberated from the plastic isolations. For bringing copper in solution, a bacterially assisted agitative leaching with mixed consortium of mesophylic microorganisms has been chosen. Continuous bacterial adaptation of the cultures to the substrate has been envisaged in order to provide an efficient way for ferrous iron regeneration during the leaching. It has been established that under optimal conditions of pH, density and temperature it is possible to recover nearly the total copper within short leach duration. The obtained pregnant leach solution could be subjected to subsequent copper recovery via solvent extraction, while the solid leached residue could be considered as non-metallic material containing plastics suitable for recycling. [less ▲] Detailed reference viewed: 85 (11 ULg) Biospheric carbon stocks reconstructed at the Last Glacial Maximum: comparison between general circulation models using prescribed and computed sea surface temperatures; ; et al in Global and Planetary Change (2002), 33(1-2), 117-138 The terrestrial biosphere model Carbon Assimilation in the Biosphere (CARAIB) was improved by introducing two vegetation storeys and implementing a new module which simulates the equilibrium distribution ... [more ▼] The terrestrial biosphere model Carbon Assimilation in the Biosphere (CARAIB) was improved by introducing two vegetation storeys and implementing a new module which simulates the equilibrium distribution of the vegetation inferred from physiological processes and climatic constraints. In this fourth version of CARAIB, we differentiate ground-level grasses from tree canopies, which allows us to determine the light available to grasses as a direct function of the leaf area index (LAI) of the forest canopy. Both of these storeys are potentially composed of several plant functional types (PFT). The cover fraction of each PFT within each storey is estimated according to its respective net primary productivity (NPP). A biome is assigned to each grid cell on the basis of three physiological criteria: (1) the cover fraction, (2) the NPP, and (3) the LAI; and two climatic constraints: (1) the growing degree-days (GDD) and (2) the lowest temperature reached during the cold season (T-min), which are well-known indices of vegetation expansion boundaries. Total biospheric carbon stocks (vegetation + soil) are reconstructed by forcing the model with eight climatic scenarios of the Last Glacial Maximum (LGM, 21 ka BP), which were obtained from the Palco-Modelling Intercomparison Project (PMIP) from four general circulation models (MRI2, UGAMP, LMD4, and GEN2) using prescribed and computed sea surface temperatures (SSTs). The model was also forced with a current climate together with a preindustrial atmospheric CO2 level of 280 ppm as reference simulation, To validate the model, current biome distribution is reconstructed and compared, for the modem climate, with two distributions of potential vegetation and, for the LGM, with pollen data. The model simulations are in good agreement with broad-scale patterns of vegetation distribution, The results indicate an increase in the total biospheric carbon stock of 827.8-1106.1 Gt C since the LGM. Sensitivity analyses were performed to discriminate the relative effects of the atmospheric CO, level ("fertilization effect"), the climate (present or LGM), and the sea level. Our results suggest that the CO, fertilization effect is mostly responsible for the total increase in vegetation and soil carbon stocks. The four GCMs diverged in their predicted responses of continental climate to calculated SSTs. Only one of them, i.e., MRI2, predicted a marked decline of the continental temperatures in response to lower calculated SSTs. For this GCM, the effect of reduced SSTs on continental biospheric carbon stocks was a decrease of 544.1 Gt for the soil carbon stock and of 283.7. [less ▲] Detailed reference viewed: 8 (2 ULg) |
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