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See detailAsporin Is a Fibroblast-Derived TGF-beta1 Inhibitor and a Tumor Suppressor Associated with Good Prognosis in Breast Cancer.
Maris, Pamela; Blomme, Arnaud; Palacios, Ana Perez et al

in PLoS medicine (2015), 12(9), 1001871

BACKGROUND: Breast cancer is a leading malignancy affecting the female population worldwide. Most morbidity is caused by metastases that remain incurable to date. TGF-beta1 has been identified as a key ... [more ▼]

BACKGROUND: Breast cancer is a leading malignancy affecting the female population worldwide. Most morbidity is caused by metastases that remain incurable to date. TGF-beta1 has been identified as a key driving force behind metastatic breast cancer, with promising therapeutic implications. METHODS AND FINDINGS: Employing immunohistochemistry (IHC) analysis, we report, to our knowledge for the first time, that asporin is overexpressed in the stroma of most human breast cancers and is not expressed in normal breast tissue. In vitro, asporin is secreted by breast fibroblasts upon exposure to conditioned medium from some but not all human breast cancer cells. While hormone receptor (HR) positive cells cause strong asporin expression, triple-negative breast cancer (TNBC) cells suppress it. Further, our findings show that soluble IL-1beta, secreted by TNBC cells, is responsible for inhibiting asporin in normal and cancer-associated fibroblasts. Using recombinant protein, as well as a synthetic peptide fragment, we demonstrate the ability of asporin to inhibit TGF-beta1-mediated SMAD2 phosphorylation, epithelial to mesenchymal transition, and stemness in breast cancer cells. In two in vivo murine models of TNBC, we observed that tumors expressing asporin exhibit significantly reduced growth (2-fold; p = 0.01) and metastatic properties (3-fold; p = 0.045). A retrospective IHC study performed on human breast carcinoma (n = 180) demonstrates that asporin expression is lowest in TNBC and HER2+ tumors, while HR+ tumors have significantly higher asporin expression (4-fold; p = 0.001). Assessment of asporin expression and patient outcome (n = 60; 10-y follow-up) shows that low protein levels in the primary breast lesion significantly delineate patients with bad outcome regardless of the tumor HR status (area under the curve = 0.87; 95% CI 0.78-0.96; p = 0.0001). Survival analysis, based on gene expression (n = 375; 25-y follow-up), confirmed that low asporin levels are associated with a reduced likelihood of survival (hazard ratio = 0.58; 95% CI 0.37-0.91; p = 0.017). Although these data highlight the potential of asporin to serve as a prognostic marker, confirmation of the clinical value would require a prospective study on a much larger patient cohort. CONCLUSIONS: Our data show that asporin is a stroma-derived inhibitor of TGF-beta1 and a tumor suppressor in breast cancer. High asporin expression is significantly associated with less aggressive tumors, stratifying patients according to the clinical outcome. Future pre-clinical studies should consider options for increasing asporin expression in TNBC as a promising strategy for targeted therapy. [less ▲]

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See detailL'assainissement viral des bananiers: une étape primordiale dans la préservation de la biodiveristé
Lassois, Ludivine ULg; Castaigne, Michel; D'haes, François

Diverse speeche and writing (2010)

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite ... [more ▼]

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite collaboration avec Bioversity International afin de fournir au Centre de Transit International, et à fortiori à la communauté internationale, des bananiers indemnes de virus. Les bananiers ainsi obtenus peuvent donc être diffusés largement sans craintes d’une épidémie quelconque. Ce travail d’assainissement contribue non seulement à la sauvegarde de la biodiversité mais également à sa valorisation en rendant saines des variétés qui étaient virosées et non exploitables. Jusqu’à présent l’ITC a fourni 15 000 plantules saines de bananiers dans 335 régions réparties dans 100 pays. Ce travail conjoint de conservation et d’assainissement permet de valoriser la grande diversité génétique du bananier au service de la communauté internationale. [less ▲]

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See detailAssay for radiolabeled type IV collagen in the presence of other proteins using a specific collagenase.
Garbisa, S.; Tryggvason, K.; Foidart, Jean-Michel ULg et al

in Analytical Biochemistry (1980), 107(1), 187-92

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See detailAssay of Matrix Metalloproteinases Types 1, 2, 3 and 9 in Breast Cancer
Remacle, A. G.; Noël, Agnès ULg; Duggan, C. et al

in British Journal of Cancer (1998), 77(6), 926-31

Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast ... [more ▼]

Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated forms, i.e. 62 kDa and 59 kDa, were found in three (6%) and 34 (40%) samples respectively. In contrast to MMP-2, most of the samples (52%) contained MMP-9 in its precursor form. Using ELISA, MMP-1 levels were found in 12% of the samples while MMP-3 levels were found in only 2% of the samples. Levels of MMP-2, -3 and -9 correlated inversely with numbers of nodal metastases. Neither MMP-2 nor -9 levels were significantly related to patient outcome. However, patients with high levels of a 50-kDa gelatinase band after zymography had a significantly better survival than patients with low levels. This species was never observed in normal breast tissue. [less ▲]

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See detailAssemblage de tôles minces par clinchage en construction métallique
Pietrapertosa, Carlo; Habraken, Anne ULg; Jaspart, Jean-Pierre ULg

Report (2005)

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See detailAssemblage moléculaire électro-assisté d’organothiols mono- et bipodes sur cuivre
Maho, Anthony ULg; Denayer, Jessica; Delhalle, Joseph et al

Conference (2010)

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See detailAssemblage-moléculaire électro-assisté d'organothiols sur cuivre polycristallin
Maho, Anthony ULg; Denayer, Jessica ULg; Delhalle, Joseph et al

in Chimie Nouvelle (2011), 5

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See detailAssembly and concept of a web-based GIS within the paleolimnological project CONTINENT (Lake Baikal, Russia)
Heim, Birgit; Klump, Jens; Oberhaensli, Hedi et al

in Journal of Paleolimnology (2008), 39(4), 567-584

Web-based Geographical Information Systems (GIS) are excellent tools within interdisciplinary and multi-national geoscience projects to exchange and visualize project data. The web-based GIS presented in ... [more ▼]

Web-based Geographical Information Systems (GIS) are excellent tools within interdisciplinary and multi-national geoscience projects to exchange and visualize project data. The web-based GIS presented in this paper was designed for the paleolimnological project 'High-resolution CONTINENTal paleoclimate record in Lake Baikal' (CONTINENT) (Lake Baikal, Siberia, Russia) to allow the interactive handling of spatial data. The GIS database combines project data (core positions, sample positions, thematic maps) with auxiliary spatial data sets that were downloaded from freely available data sources on the world wide web. The reliability of the external data was evaluated and suitable new spatial datasets were processed according to the scientific questions of the project. GIS analysis of the data was used to assist studies on sediment provenance in Lake Baikal, or to help answer questions such as whether the visualization of present-day vegetation distribution and pollen distribution supports the conclusions derived from palynological analyses. The refined geodata are returned back to the scientific community by using online data publication portals. Data were made citeable by assigning persistent identifiers (DOI) and were published through the German National Library for Science and Technology (TIB Hannover, Hannover, Germany). [less ▲]

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See detailThe assembly of a farm meat network in Belgium and risk
Stassart, Pierre M ULg

Conference (2001)

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See detailAssembly of enveloped RNA viruses
Dubois-Dalcq, Monique; Holmes, Kathryn V; Rentier, Bernard ULg

Book published by Springer Verlag (1985)

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See detailAssembly of Ligand-Stripped Nanocrystals into Precisely Controlled Mesoporous Architectures
Buonsanti, R.; Pick, T. E.; Krins, Natacha ULg et al

in Nano Letters (2012), 12

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See detailAssesment of nuclear totipotency of fetal bovine diploid germ cells by nuclear transfer
Moens, André; Chesné, Patrick; Delhaize, Françoise et al

in Theriogenology (1996), 46

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See detailAssesment of the situation, three years later, on 'the Belgian Dioxin Crisis'. Probable impact on the health of the Belgian population
Focant, Jean-François ULg; Pirard, Catherine ULg; Douny, Caroline ULg et al

in Annales de Médecine Vétérinaire (2002), 146(6, DEC-JAN), 321-327

In January 1999, Belgium encounters an accidental contamination of the food chain, which was named "the Belgian Dioxin Crisis". The aim of the present review is to estimate the impact of this crisis on ... [more ▼]

In January 1999, Belgium encounters an accidental contamination of the food chain, which was named "the Belgian Dioxin Crisis". The aim of the present review is to estimate the impact of this crisis on public health. Currently, it does not really exist any exhaustive epidemiological study. Various scientific studies actually report somewhat conflicting results. As an example, concerning the population living in Wallonia, recorded dioxin levels are not higher than background levels reported for other industrialized countries, despite the 1999 "dioxin crisis". Conversely, based on levels measured in foodstuffs collected by the end of the crisis period, the dioxin body burden of potential consumers would have increased in the range of 40 to 75 %. The real impact of this crisis on consumers' health could thus only be estimated through well structured epidemiological campaigns. [less ▲]

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See detailAssessement of risk factors for osteoporotic fractures in elderly men
Scholtissen, S.; Guillemin, F.; Kemmer, C. et al

in Osteoporosis International (2008, April), 19(Suppl.1), 200-201

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See detailAssessing a river floodplain status using airborne imaging spectrometer data and ground validation – The HyEco'04 project
Kooistra, L; Batelaan, O; Clevers, J et al

in Abstract for Third Airborne Imaging Spectroscopy Workshop in Bruges (2005)

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See detailAssessing and aggregating trends in groundwater bodies. Examples of the FP VI Aquaterra-project
Broers, Hans-peter; Visser, Ate; Brouyère, Serge ULg et al

in EU Groundwater Policy Development - Good Status Objectives and Integrated management planning (2008, November)

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See detailAssessing and Harmonizing Lying Deadwood Volume with Regional Forest Inventory Data in Wallonia (Southern Region of Belgium)
Ligot, Gauthier ULg; Lejeune, Philippe ULg; Rondeux, Jacques ULg et al

in Open Forest Science Journal (2012), 5

Using lying deadwood or coarse woody debris (CWD) is on the rise among national and local forest inventories. We compared two generally recommended methods for lying deadwood: line intersect sampling and ... [more ▼]

Using lying deadwood or coarse woody debris (CWD) is on the rise among national and local forest inventories. We compared two generally recommended methods for lying deadwood: line intersect sampling and fixed area sampling. We assessed both methods in plots of the regional forest inventory of Wallonia (southern region of Belgium) and we developed bridging functions that converts CWD volume estimates to estimates that would have been obtained with different thresholds. [less ▲]

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See detailAssessing and modeling economic and environmental impact of wheat nitrogen management in Belgium
Dumont, Benjamin ULg; Basso, Bruno; Bodson, Bernard ULg et al

in Environmental Modelling & Software (2016), 79

Future progress in wheat yield will rely on identifying genotypes and management practices better adapted to the fluctuating environment. Nitrogen (N) fertilization is probably the most important practice ... [more ▼]

Future progress in wheat yield will rely on identifying genotypes and management practices better adapted to the fluctuating environment. Nitrogen (N) fertilization is probably the most important practice impacting crop growth. However, the adverse environmental impacts of inappropriate N management (e.g., lixiviation) must be considered in the decision-making process. A formal decisional algorithm was developed to tactically optimize the economic and environmental N fertilization in wheat. Climatic uncertainty analysis was performed using stochastic weather time-series (LARS-WG). Crop growth was simulated using STICS model. Experiments were conducted to support the algorithm recommendations: winter wheat was sown between 2008 and 2014 in a classic loamy soil of the Hesbaye Region, Belgium (temperate climate). Results indicated that, most of the time, the third N fertilization applied at flag-leaf stage by farmers could be reduced. Environmental decision criterion is most of the time the limiting factor in comparison to the revenues expected by farmers. [less ▲]

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