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See detailAutomated Phase Map Referencing Against Historic Map Data
Groves, Roger; De Rauw, Dominique ULg; Thizy, Cédric ULg et al

in Osten, W.; Kujawinska, M. (Eds.) Fringe 2009 (2009, September)

Holography and shearography techniques are in common use for precise measurements in high technology industries, such as aerospace, automotive, precision manufacturing and for the structural diagnostics ... [more ▼]

Holography and shearography techniques are in common use for precise measurements in high technology industries, such as aerospace, automotive, precision manufacturing and for the structural diagnostics of artwork. The phase map data generated by these instruments allows precise measurements of parameters such as displacement, strain and shape. The comparison of data from different measurement campaigns is useful for null testing components and for studying changes. However, the comparison of data with previous measurements is non-trivial due to the sensitivity of these techniques to alignment and to sample loading parameters. This paper addresses image and phase registration issues for holography and shearography. This manuscript describes 2D spatial co-registration to compensate for differences between the field of view between the measurements and a phase referencing to calculate the difference between the phase maps. These techniques are implemented after following a standardised measurement procedure. [less ▲]

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See detailAutomated platform for the quality control of PET-CT scanners following AFCN/FANC recommendations
Bernard, Claire ULg; Paulus, Cyril; JODOGNE, Sébastien ULg et al

in Proceedings of the Belgian Hospital Physicists Association Symposium (2013, February)

Detailed reference viewed: 28 (15 ULg)
See detailAutomated procedures in ISO17025 routine dioxin laboratory: impact on throughput
Focant, Jean-François ULg

Scientific conference (2012, April)

Detailed reference viewed: 13 (1 ULg)
See detailAutomated procedures in ISO17025 routine: Impact on the laboratory throughput
Focant, Jean-François ULg

Scientific conference (2011, April)

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See detailAutomated Processing of Zebrafish Imaging Data: A Survey
Mikut, Ralf; Dickmeis, Thomas; Driever, Wolfgang et al

in Zebrafish (2013), 10(3), 401-421

Due to the relative transparency of its embryos and larvae, the zebrafish is an ideal model organism for bioimaging approaches in vertebrates. Novel microscope technologies allow the imaging of ... [more ▼]

Due to the relative transparency of its embryos and larvae, the zebrafish is an ideal model organism for bioimaging approaches in vertebrates. Novel microscope technologies allow the imaging of developmental processes in unprecedented detail, and they enable the use of complex image-based read-outs for high-throughput/high-content screening. Such applications can easily generate Terabytes of image data, the handling and analysis of which becomes a major bottleneck in extracting the targeted information. Here, we describe the current state of the art in computational image analysis in the zebrafish system. We discuss the challenges encountered when handling high-content image data, especially with regard to data quality, annotation, and storage. We survey methods for preprocessing image data for further analysis, and describe selected examples of automated image analysis, including the tracking of cells during embryogenesis, heartbeat detection, identification of dead embryos, recognition of tissues and anatomical landmarks, and quantification of behavioral patterns of adult fish. We review recent examples for applications using such methods, such as the comprehensive analysis of cell lineages during early development, the generation of a three-dimensional brain atlas of zebrafish larvae, and high-throughput drug screens based on movement patterns. Finally, we identify future challenges for the zebrafish image analysis community, notably those concerning the compatibility of algorithms and data formats for the assembly of modular analysis pipelines. [less ▲]

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See detailAutomated Production of Potassium-38 for the Study of Myocardial Perfusion Using Positron Emission Tomography
Guillaume, Marcel; de Landsheere, Christian ULg; Rigo, Pierre ULg et al

in International Journal of Radiation Applications and Instrumentation. Part A : Applied Radiation and Isotopes (1988), 39(2), 97-107

The 35Cl(alpha, n)38K nuclear reaction was developed for the routine cyclotron production of repeated multimillicurie batches of 38K for dynamic studies of regional tissue perfusion with positron emission ... [more ▼]

The 35Cl(alpha, n)38K nuclear reaction was developed for the routine cyclotron production of repeated multimillicurie batches of 38K for dynamic studies of regional tissue perfusion with positron emission tomography. The objective is to make this isotope available as an alternative to the use of [13N]NH3 and 82Rb for the investigation of flow in experimental and clinical use. A sodium chloride powder target mounted on a water-cooled nickel backing is bombarded with 26 MeV helium-4 ions at a maximum beam current of 25 microA. The target is remotely disconnected from the bombardment port, transported to a hot cell and entirely processed by a computer-controlled system within a total time of less than 4 min. The final pyrogen-free isotonic and sterile solution of 38K has a radionuclidic purity of more than 99.99%. A typical yield of 19 +/- 2 mCi of 38K is obtained at the end of a 30 min bombardment at 10 microA. The production rate at saturation can therefore be estimated to 2.05 +/- 0.2 mCi/microA. [less ▲]

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See detailAutomated quantification of creatine kinase (CK)-MB by a new fluorometric enzyme immunoassay
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1989, June)

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See detailAutomated quantification of creatine kinase-MB in serum by radial partition immunoassay
Chapelle, Jean-Paul ULg; El Allaf, M.

Conference (1989, August)

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See detailAutomated quantification of creatinine kinase MB isoenzyme in serum by radial partition immunoassay, with use of the Stratus analyzer
Chapelle, Jean-Paul ULg; El Allaf, M.

in Clinical Chemistry (1990), 36(1), 99-101

We evaluated the analytical and clinical performances of a new radial partition immunoassay for measuring the mass concentration of creatine kinase (CK)-MB in serum. All pipetting, washes, incubations and ... [more ▼]

We evaluated the analytical and clinical performances of a new radial partition immunoassay for measuring the mass concentration of creatine kinase (CK)-MB in serum. All pipetting, washes, incubations and data reduction were performed in 8 min by the Stratus (Dade) fluorometric analyzer. Within-assay and between-assay CVs were respectively 5.5% and 8.4% at 21 micrograms/L, and 4.2% and 3.4% at 48 micrograms/L. Assaying serial dilutions of serum samples with high CK-MB concentrations demonstrated excellent linearity. Results of the Stratus technique correlated well (n = 115, r = 0.98) with those of the Tandem-E CKMB II assay. There was no interference from hemolysis, bilirubin, rheumatoid factor, or added CK-MM (up to 3500 U/L); consequently, CK-MB can be determined in undiluted serum, even in the presence of high total CK activity. The mean CK-MB concentration in 105 blood donors was 1.9 (SD 1.3) micrograms/L. For seven myocardial infarction patients who received prompt fibrinolytic therapy, the mean CK-MB concentration was 4.5 (SD 1.8) micrograms/L at admission, and maximum concentrations, 119 (SD 94) micrograms/L, were recorded 16 h later. CK-MB returned to concentrations less than 10 micrograms/L within 72 h. [less ▲]

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See detailAutomated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors
Goblet, David ULg; Thonon, David ULg; Plenevaux, Alain ULg et al

Poster (2010, May 30)

TOPIC: Molecular Neuroimaging: from Bench to Bedside Automated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors Introduction: Dysfunction of the cerebral serotoninergic system is ... [more ▼]

TOPIC: Molecular Neuroimaging: from Bench to Bedside Automated radiosynthesis of [18F]MPPF derivatives for imaging 5-HT1A receptors Introduction: Dysfunction of the cerebral serotoninergic system is implicated in numerous neurodegenerative disorders such as Alzheimer disease’s, dementia, depression, anxiety, schizophrenia, and Parkinson disease’s. The 5-HT1A serotonin receptors are involved in several physiological functions including sleep, mood, neurogenesis and learning [1]. Consequently, there have been huge efforts in finding ligands for this receptor. [11C]WAY-100635 is a high affinity radioligand used for quantifying serotonin 5-HT1A receptors with positron emission tomography. An 18F-labeled radioligand is advantageous because of higher specific activity and physical/nuclear properties (t1/2= 109 min, 97% of positron decay and positron energy of 635 keV maximum). [18F]MPPF, a selective 5-HT1A antagonist derived from WAY-100635, is currently one of the most successful PET ligand used for 5-HT1A receptor imaging [2]. However the affinity is lower then WAY-100635 and the amount of [18F]MPPF reaching the brain is relatively low since MPPF is a substrate for p-glycoprotein [3]. Methods: In order to improve the brain uptake of the radiotracer, a desmethylated analog has been developed in our lab and preliminary in vitro studies show positive results [4]. Nevertheless, the radiosynthesis take place in two steps as a protecting group removal is needed. A one step procedure with a MPPF derivative could be of very great interest. We have synthesized many MPPF derivatives in our lab (modification on the phenylpiperazine moiety) and developed an automated radiosynthesis procedure for the production of these radiotracers. [18F]MPPF was chosen as the model compound. We used a GE Healthcare FASTlabTM module and made modifications to the [18F]FDG synthesis sequence and cassette. [18F]MPPF was synthesized by coupling of [18F]FBA with the corresponding amine. After coupling, the crude solution was diluted with water and passed through a tC18 cartridge for prepurification. After elution, the [18F]MPPF was purified by semi-preparative HPLC. Results: Total synthesis time, including purification was approximately 100 min. [18F]FBA and [18F]MPPF were obtained at a corrected yield of 55% (n=20) and 25% (n=5) respectively. The radiochemical purity, checked by radio-TLC and UPLC, was >95%. Conclusions: We have developed an automated method for [18F]MPPF and derivatives production using a commercial synthesizer (FASTlabTM from GE Healthcare) and a conventional HPLC system resulting in good yields and high (radio)chemical purity. By simply switching the vial containing the modified amine, an 18F-labeled MPPF derivative could be obtained. Radiosynthesis is still under optimization and the radiotracers synthesized need to be tested as suitable 5-HT1A radioligands. Acknowledgement: This work was supported by the Fondation Rahier. References: [1] Filip M., Bader M. et Al, Pharmacol Rep. 2009 Sep-Oct; 61(5):761-77 [2] Aznavour N, Zimmer L. Et Al, Neuropharmacology. 2007 Mar; 52(3):695-707 [3] Laćan G., Plenevaux A. et Al, Eur J Nucl Med Mol Imaging. 2008 Dec;35(12):2256-66 [4] Defraiteur C., Plenevaux A. et Al., Br J Pharmacol. 2007 Nov; 152(6):952-8 [less ▲]

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See detailAutomated respiratory cycles selection improves the variability of respiratory mechanics monitoring.
Rigo, Vincent ULg; Graas, Estelle; Rigo, Jacques ULg

in Rimensberger, Peter (Ed.) 10th European Conference on Pediatric and Neonatal Ventilation program (2010, April)

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See detailAutomated respiratory cycles selection is highly specific and improves respiratory mechanics analysis.
RIGO, Vincent ULg; Graas, Estelle ULg; Rigo, Jacques ULg

in Pediatric Critical Care Medicine (2012), 13(4), 234-9

OBJECTIVE:: Selected optimal respiratory cycles should allow calculation of respiratory mechanic parameters focusing on patient-ventilator interaction. New computer software automatically selecting ... [more ▼]

OBJECTIVE:: Selected optimal respiratory cycles should allow calculation of respiratory mechanic parameters focusing on patient-ventilator interaction. New computer software automatically selecting optimal breaths and respiratory mechanic derived from those cycles are evaluated. DESIGN:: Retrospective study. SETTING:: University level III neonatal intensive care unit. SUBJECTS:: Ten mins synchronized intermittent mandatory ventilation and assist/control ventilation recordings from ten newborns. INTERVENTION:: The ventilator provided respiratory mechanic data (ventilator respiratory cycles) every 10 secs. Pressure, flow, and volume waves and pressure volume, pressure flow, and ventilator volume flow loops were reconstructed from continuous pressure/volume recordings. Visual assessment determined assisted leak-free optimal respiratory cycles (selected respiratory cycles). New software graded the quality of cycles (automated respiratory cycles). Respiratory mechanic values were derived from both sets of optimal cycles. We evaluated quality selection and compared mean values and their variability according to ventilatory mode and respiratory mechanic provenance. To assess discriminating power, all 45 "t" values obtained from interpatient comparisons were compared for each respiratory mechanic parameter. MEASUREMENTS AND MAIN RESULTS:: A total of 11,724 breaths are evaluated. automated respiratory cycle/selected respiratory cycle selections agreement is high: 88% of maximal kappa with linear weighting. Specificity and positive predictive values are 0.98 and 0.96, respectively. Averaged values are similar between automated respiratory cycle and ventilator respiratory cycle. C20/C alone is markedly decreased in automated respiratory cycle (1.27 +/- 0.37 vs. 1.81 +/- 0.67). Tidal volume apparent similarity disappears in assist/control: automated respiratory cycle tidal volume (4.8 +/- 1.0 mL/kg) is significantly lower than for ventilator respiratory cycle (5.6 +/- 1.8 mL/kg). Coefficients of variation decrease for all automated respiratory cycle parameters in all infants. "t" values from ventilator respiratory cycle data are two to three times higher than ventilator respiratory cycles. CONCLUSIONS:: Automated selection is highly specific. Automated respiratory cycle reflects most the interaction of both ventilator and patient. Improving discriminating power of ventilator monitoring will likely help in assessing disease status and following trends. Averaged parameters derived from automated respiratory cycles are more precise and could be displayed by ventilators to improve real-time fine tuning of ventilator settings. [less ▲]

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See detailAutomated Sample Preparation for Same-Day POP Analysis
Focant, Jean-François ULg

Scientific conference (2011, November)

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See detailAutomated Sample Preparation Techniques for the Determination of Drug Enantiomers in Biological Fluids Using Liquid Chromatography with Chiral Stationary Phases
Ceccato, Attilio ULg; Toussaint, B.; Chiap, Patrice ULg et al

in Enantiomer (1999), 4(3-4), 305-15

The determination of drug enantiomers has become of prime importance in the field of pharmaceutical and biomedical analysis. Liquid chromatography (LC) is one of the most frequently used techniques for ... [more ▼]

The determination of drug enantiomers has become of prime importance in the field of pharmaceutical and biomedical analysis. Liquid chromatography (LC) is one of the most frequently used techniques for achieving the separation and quantitation of the enantiomers of drug compounds. In the bioanalytical field, the integrated systems present an interesting alternative to time-consuming sample preparation techniques such as liquid-liquid extraction. Solid phase extraction (SPE) on disposable cartridges, dialysis or column switching are sample preparation techniques that can be fully automated and applied to enantioselective analysis in biological fluids. The selection of the most appropriate LC mode and chiral stationary phase for enantioseparations in bioanalysis is discussed and some aspects of these automated sample preparation procedures are compared, such as selectivity, detectability, elution of the analytes from the extraction sorbent, sample volume and analyte stability. [less ▲]

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See detailAutomated sample preparation-fractionation for the measurement of dioxins and related compounds in biological matrices: a review
Focant, Jean-François ULg; Pirard, Catherine; De Pauw, Edwin ULg

in Talanta (2004), 63(5), 1101-1113

This article reviews some of the recent developments in the extraction and clean-up areas of biological samples dedicated to dioxin and related compound analysis. A brief introduction on the major dioxin ... [more ▼]

This article reviews some of the recent developments in the extraction and clean-up areas of biological samples dedicated to dioxin and related compound analysis. A brief introduction on the major dioxin contamination events, which have occurred in the food chain, is given to illustrate the need of fast high throughput methods in case of crises. The emphasis of this paper is the method development based upon reliable instrumental extraction techniques for rapid sample processing and automation such as; supercritical fluid extraction (SFE), microwave-assisted extraction (MAE), pressurized liquid extraction (PLE) and, solid-phase extraction (SPE). The PLE and SPE are also discussed in conjunction with the use of a multi-column automated clean-up system that can accommodate up to 5 g of extracted lipids. The fractionation in sub-groups of analytes during the clean-up process allows the isolation of various types of toxicants from a single sample and illustrates the versatility of the system. An integrated extraction and clean-up instrument is finally presented in terms of feasibility and attainable sample turnover for the parallel processing of liquid and solid biological samples. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailAutomated SPE and clean-up for PBDEs in human blood
Focant, Jean-François ULg

Scientific conference (2011, June)

Detailed reference viewed: 4 (0 ULg)