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See detailQuantification of lipid bilayer viscosity and fuidity effect induced by propofol.
Bahri, Mohamed Ali ULg; Heyne, Belinda; Hans, Pol ULg et al

Conference (2004, April 23)

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Microbiology (2006), 114(3-4), 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We ... [more ▼]

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailQuantification of mRNA encoding cytokines, chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneumopathy.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailQuantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reaction
Duchatel, Jean-Pierre ULg; Todd, D; Willeman, C et al

in Avian Pathology : Journal of the W.V.P.A (2009), 38(2), 143-148

The development of a real-time polymerase chain reaction (PCR) based on SYBR Green chemistry is described for the quantification of pigeon circovirus (PiCV) DNA in various samples. Plasmid containing a ... [more ▼]

The development of a real-time polymerase chain reaction (PCR) based on SYBR Green chemistry is described for the quantification of pigeon circovirus (PiCV) DNA in various samples. Plasmid containing a fragment of the PiCV genome was used to create a standard curve and to estimate the viral DNA copies in analysed samples. Both primers were designed in highly conserved regions to avoid false negatives, and amplified a 139-base-pair amplicon. When the amplifications were performed in the presence of cellular DNA extracted from PCR-negative liver, bursa and spleen samples, the detection limits were respectively 20, 20 and 60 copies of genome per milligram of tissue. These limits were 10, 160 and 25 copies/ml for control blood, sera and semen, respectively. For cloacal swab, the detection limit was 200 copies. The assay showed a <br />linear detection over a six-log range (R2 0.99) and displayed reliable inter-assay and intra-assay reproducibility. Application of the test to sera samples indicated the presence of the virus in Belgium in 1991, 6 years before PiCV infections were histologically diagnosed. Testing of samples from pigeons with <br />‘‘young pigeon sickness’’ showed that the viral loads were high in the bursa of Fabricius (up to 2.07 x 10^9 copies/mg), the liver (up to 2.88x10^8 copies/mg) and spleen (up to 5.57x10^8 copies/mg). For liver, the viral load was significantly higher in sick pigeons than in apparently healthy pigeons. Detection of high quantities of PiCV DNA (up to 1.6x10^9 copies/ml) in the sera or blood of some young healthy pigeons <br />indicated that the viral load in this sample type would not be useful as predictive indicator of disease. This work also showed that PiCV DNA can be detected in relatively large amounts in semen (up to 1.0x10^7 copies/ejaculate) and cloacal swabs (up to 3.6x10^10 copies/swab), confirming that PiCV may be transmitted by vertical and horizontal routes. [less ▲]

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See detailQuantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Grammenos, Angeliki ULg; Bahri, Mohamed Ali ULg; Guelluy, Pierre-Henri ULg et al

in Biochemical and Biophysical Research Communications (2009), 390

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance ... [more ▼]

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9°C). [less ▲]

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See detailQuantification of rotenone in seeds of different species of yam bean (Pachyrhizus sp.) by a SPE HPLC-UV method
Lautié, E.; Rozet, Eric ULg; Hubert, Philippe ULg et al

in Food Chemistry (2012), 131(4), 1531-1538

This study describes the development of a validated method for the quantification of rotenone in yam bean. The milled seeds were submitted to a Soxhlet dichloromethane extraction which allowed extracting ... [more ▼]

This study describes the development of a validated method for the quantification of rotenone in yam bean. The milled seeds were submitted to a Soxhlet dichloromethane extraction which allowed extracting 90% of the seeds rotenone. Elimination of the lipids was obtained via solid phase extraction. Rotenone was eluted with dichloromethane/methanol and the solution dried under vacuum and solubilised directly in methanol before injection in HPLC. The whole process was realised as much as possible protected from light and at temperatures lower than 40°C which allowed high recovery rates of spiked rotenone. Total error was used as criterion for the validation process and accuracy profiles drawn. The method allows the quantification of rotenone in yam bean seeds from 0.07% up to 1.25% (w/w). This method was applied to the quantification of rotenone in the seeds of several accessions of Pachyrhizus erosus and P. ahipa. The results range from 1.13 to 2.76 mg/g dry material. [less ▲]

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See detailQuantification of skin color in patients undergoing maintenance hemodialysis
Deleixhe-Mauhin, F.; Krzesinski, Jean-Marie ULg; Rorive, Georges ULg et al

in Journal of the American Academy of Dermatology (1992), 27(6), 950-953

Background: In patients undergoing long-term hemodialysis, a peculiar hyperpigmentation develops, the intensity of which may or may not be related to the duration of treatment and use of erythropoietin ... [more ▼]

Background: In patients undergoing long-term hemodialysis, a peculiar hyperpigmentation develops, the intensity of which may or may not be related to the duration of treatment and use of erythropoietin. Objective: Our purpose was to conduct a comparative cross-sectional study of white patients with distinct diseases that modify their skin color. Methods: A reflected-light colorimeter was used to compare the color of the forehead and forearms of 61 white patients receiving hemodialysis with that of matched controls and of patients with anemia or icterus. Results: Significant differences were found in both reflectance and chromaticity between these groups of patients. Duration of hemodialysis and use of erythropoietin did not significantly influence the colorimetric measurements. Conclusion: The use of a color-reflectance meter allows precise evaluation of subtle changes in skin color and may be used to monitor several diseases. [less ▲]

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See detailQuantification of T cell receptor rearrangement excision circles to estimate thymic function: an important new tool for endocrine-immune physiology
Geenen, Vincent ULg; Poulin, J. F.; Dion, M. L. et al

in Journal of Endocrinology (2003), 176(3), 305-311

Although the thymus constitutes a target organ for most protein and steroid hormones, it has been quite difficult to determine the precise control exerted in vivo by the endocrine system upon thymic ... [more ▼]

Although the thymus constitutes a target organ for most protein and steroid hormones, it has been quite difficult to determine the precise control exerted in vivo by the endocrine system upon thymic function. The biological role of the thymus is to ensure the generation of a diversified population of peripheral T cells able to respond to non-self-antigens but nevertheless tolerant to self-antigens. For a long time, thymic function could not be monitored, as a consequence of the absence of adequate technology to differentiate recent thymic emigrants from naive T cells. The generation of T cell receptor (TCR) diversity occurs in the thymus through recombination of gene segments encoding the variable parts of the TCR alpha and beta chains. During these processes, by-products of the rearrangements are generated in the form of TCR excision circles (TRECs). As these molecules are lost upon further cell division, their quantification is actually considered as a very valuable tool to estimate thymic function. The most appropriate TREC is deltaRec-Psi(J)alpha TREC or signal joint TREC resulting from deltaRec-Psi(J)alpha rearrangement (TCRD deletion) that occurs late during thymopoiesis, before V(alpha)-J(alpha) rearrangement. Here we describe how TREC quantification is a powerful and reliable method to evaluate the impact of hormones and endocrine disorders upon thymic function [less ▲]

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See detailQuantification of tagitinin C in Tithonia diversifolia by reversed-phase high-performance liquid chromatography.
Goffin, Eric ULg; da Cunha, Antonio Proenca; Ziemons, Eric ULg et al

in Phytochemical Analysis [=PCA] (2003), 14(6), 378-80

A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the determination of tagitinin C, an anti-plasmodial sesquiterpene lactone isolated from the aerial parts of ... [more ▼]

A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the determination of tagitinin C, an anti-plasmodial sesquiterpene lactone isolated from the aerial parts of Tithonia diversifolia, has been developed. The assay has been used to quantify tagitinin C in various extracts of the aerial parts of T. diversifolia. [less ▲]

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See detailQuantification of the Carbonate Pump: Case study of an Emiliania huxleyi bloom in the Bay of Biscay
Harlay, Jérôme ULg; Chou, Lei; Roevros, Nathalie et al

Poster (2003, April 03)

Little attention has been paid until now to the processes controlling the production, dissolution and fate of biogenic calcium carbonate in the oceans. It is however well known that net deposition rates ... [more ▼]

Little attention has been paid until now to the processes controlling the production, dissolution and fate of biogenic calcium carbonate in the oceans. It is however well known that net deposition rates of inorganic carbon to the sediments are comparable to those of organic matter. There remains still large uncertainties in the production and redissolution of biogenic carbonate in the marine system and thus about the role of the carbonate pump in response to anthropogenic CO2 perturbations. The understanding of these processes is also a prerequisite to predict the response of marine organisms to global environmental changes. In the framework of the Belgian global change programme, we have developed a project devoted to the study of the inorganic carbon cycle in the Bay of Biscay where coccolithophorid blooms occur frequently. The study focuses on processes associated with the oceanic production and dissolution of calcium carbonate, by combining field investigations, laboratory experiments and modelling efforts. Remote sensing demonstrates a close relationship between vertical mixing along the continental margin and the development of the phytoplankton bloom. We will present here, results of 14C incorporation experiments used to evaluate the rate of production of organic and inorganic particulate carbon, obtained during a coccolithophorid spring bloom in the investigated area. A tentative mass balance of the carbon fluxes for this area will be presented, confirming the importance that the calcium carbonate pump may play in the oceanic system. [less ▲]

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See detailQuantification of the nitrogen budget in the Black Sea using a 3D coupled hydrodynamical-biogeochemical model
Grégoire, Marilaure ULg; Nezlin, N.; Kostianoy, A. et al

Conference (2000)

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See detailQuantification of the role of mechanical parameters on the bone response around loaded titanium implants
Vandamme, Katleen; Geris, Liesbet ULg; Duyck, Joke et al

in European Cells and Materials (2003), 5(2), 96-97

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See detailQuantification of the trophic pathway in the PCB contamination of two zooplanktonic species: Daphnia magna Straus and Brachionus calyciflorus Pallas
Joaquim-Justo, Célia ULg; Thomé, Jean-Pierre ULg

in Verhandlungen der Internationalen Vereinigung für Theoretische und Angewandte Limnologie = Proceedings of the International Association of Theoretical and Applied Limnology (1998), 26

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See detailQuantification of Tropospheric Activity according to Double Differences Analysis of GNSS Phases Records.
Brenot, Hugues; Lejeune, Sandrine; Wautelet, Gilles ULg et al

Conference (2007)

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See detailQuantifications en supergéométrie
Leuther, Thomas ULg

Poster (2012, February)

Le poster présente de façon vulgarisée certaines idées sous-jacentes à la recherche de quantifications invariantes en supergéométrie.

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See detailQuantifications équivariantes
Radoux, Fabian ULg

Scientific conference (2011, April 15)

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