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See detailProduction et purification d'une ribonuclease A recombinante chez Escherichia coli
Charlier, Thierry ULg

Master of advanced studies dissertation (2000)

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See detailProduction et utilisation du colza en Belgique.
Cartrysse, C.; Cors, F.; Wathelet, Jean-Paul ULg

in GCIRC Bulletin (1991)

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See detailProduction laitière et nombre de traites de vaches laitières en prairie avec un robot: effets des facteurs environnementaux.
Dufrasne, Isabelle ULg; Robaye, Vincent ULg; Knapp, Emilie ULg et al

Poster (2012)

En Belgique, comme dans d'autres pays européens, le recours à la traite robotisée est en augmentation. La majorité des troupeaux traits avec un robot ne pratique pas le pâturage car les exploitants ... [more ▼]

En Belgique, comme dans d'autres pays européens, le recours à la traite robotisée est en augmentation. La majorité des troupeaux traits avec un robot ne pratique pas le pâturage car les exploitants considèrent que cette méthode n'est pas conciliable avec le pâturage. La fragmentation des parcelles est aussi un frein au pâturage. La mise en place d'un robot dans une structure mobile permet d'utiliser le même robot à l'étable en hiver et dans les prairies distantes de l'exploitation en été. L'objectif de cette étude est de tester l'effet de facteurs environnementaux, l'animal, la complémentation, la distance entre le robot et la parcelle, les numéros du jour dans la parcelle et de cycle de rotation sur la production laitière (PL) et le nombre de traites (NT). [less ▲]

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See detailProduction laitière et santé mammaire (Bio Ingénieurs)
Hanzen, Christian ULg

Learning material (2013)

Ce cours de 4 heures s'inscrit dans cadre du module "Production laitière par le ruminant" dispensé aux étudiants de master bio-ingénieur en sciences agronomiques. Les objectifs spécifiques ont été définis ... [more ▼]

Ce cours de 4 heures s'inscrit dans cadre du module "Production laitière par le ruminant" dispensé aux étudiants de master bio-ingénieur en sciences agronomiques. Les objectifs spécifiques ont été définis en accord avec le professeur Yves Beckers co-responsable du module. Ce cours a pour objectif général de définir la mammite, ses conséquences, ses signes d'alerte au niveau de l'individu et du troupeau, son approche diagnostique, ses facteurs de risque au niveau individuel et d'élevage et ses grandes lignes d'approche thérapeutique. [less ▲]

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See detailProduction lamellaire aurignacienne à Mitoc-Malu Galben, Roumanie
Noiret, Pierre ULg; Zwyns, Nicolas; Chirica, Vasile

Conference (2006, September)

Although retouched bladelets (Dufour or others) were not recovered, the Aurignacian assemblages from Mitoc-Malu Galben (Romania) probably belongs to the Krems-Dufour type Aurignacian. In the lithic ... [more ▼]

Although retouched bladelets (Dufour or others) were not recovered, the Aurignacian assemblages from Mitoc-Malu Galben (Romania) probably belongs to the Krems-Dufour type Aurignacian. In the lithic material gathered by V. chirica in the 1980s, evidence indicates the existence of bladelet production from carinated tools/cores. A small unpublished lithic assemblage from the 1990s excavations, associated with a hearth, confirms this hypothesis. The operational schemes are described and compared to those from the western and eastern Aurignacian geographic regions. [less ▲]

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See detailProduction ligneuse de la forêt wallonne, l'apport de l'inventaire permanent régional
Rondeux, Jacques ULg; Hebert, Jacques ULg; Bourland, Nils ULg et al

in Forêt Wallonne (2005), (79), 3-18

En Région wallonne, l’inventaire permanent des ressources forestières, mis en place en 1994, permet aujourd’hui de fournir des chiffres d’accroissement basés sur une méthodologie rigoureuse. Les chiffres ... [more ▼]

En Région wallonne, l’inventaire permanent des ressources forestières, mis en place en 1994, permet aujourd’hui de fournir des chiffres d’accroissement basés sur une méthodologie rigoureuse. Les chiffres donnés dans cet article permettent de mieux appréhender la capacité actuelle de production de nos forêts en se basant sur une approche scientifique et non sur des hypothèses. Les principaux résultats sont issus de mesurages réalisés entre novembre 1999 et 2001 et concernent une période d’accroissement de 5 ans. Ces mesures ont permis de se faire une idée de la production moyenne en volume de nos principales essences dont les grandes tendances sont les suivantes : • la production annuelle en volume de l’ensemble de la forêt wallonne productive est estimée à 10,1 m3/ha/an (dont 53 % de cette valeur est à charge de la forêt privée) ; • la production en volume des résineux est de 16,4 m3/ha/an et celle des feuillus s’élève à 5,4 m3/ha/an ; • les prélèvements correspondent à 65 % de la production en feuillu et à 109 % de celle des résineux, dans l’état actuel des surfaces occupées par classe d’âge ou stade de développement des peuplements. [less ▲]

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See detailProduction line real time near infrared (NIR) monitoring
Mantanus, Jérôme ULg; Ziemons, Eric ULg; Streel, Bruno et al

Conference (2009, September)

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See detailLa production littéraire des Belges francophones durant l’entre-deux-guerres. Examen d’un premier inventaire
Dozo, Björn-Olav ULg

in Mémoires du Livre = Studies in Book Culture (2010), 1(2),

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See detailProduction locale des antisérums de groupe sanguin ABO
Bamoleke Sefu, Anaclet; Tshiband-a-Tshish, Alphonse; Munlemvo Mavanga, Nana ULg et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2012), 27

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See detailProduction of L-[18F]fluoroamino acids for protein synthesis: overview and recent developments in nucleophilic synthesis
Lemaire, Christian ULg

in PET studies on Amino Acid Metabolism and Protein Synthesis (1993)

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See detailProduction of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolytica
Escamilla Garcia, E.; Aguedo, Mario ULg; Gomes, N. et al

in Journal of Molecular Catalysis B : Enzymatic (2009), 57(1-4), 22-26

3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was ... [more ▼]

3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation at low aeration (0.40 molar yields) and, to a lesser extent, at lower pH (0.15). As oxygen played a key-role, we evaluated its effect at fixed dissolved oxygen and at the pH which was the most favourable to the biotransformation (pH 4.5). At 5% and 30% dissolved oxygen, yields reached 0.50. β-Oxidation fluxes are very dependent on the presence of oxygen and conditions of accumulation of 3-hydroxy-γ-decalactone with very high yields were identified. These results are an important step in the production of the two decenolides. Moreover, they show the high dependence of β-oxidation fluxes on environmental conditions and relate these conditions to the accumulation of intermediates, results that are of interest to all the processes using yeast on lipids or alkanes. © 2008 Elsevier B.V. All rights reserved. [less ▲]

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See detailProduction of 4-hydroxydecanoic acid, a g-decalactone precursor, by the psychrophilic yeast Rhodotorula aurantiaca A16.
Belot, J. L.; Lognay, Georges ULg; Marlier, M. et al

Poster (1997, August)

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See detailProduction of a high percentage of male offspring with a natural androgen, 11 beta-hydroxyandrostenedione (11 beta OHA4), in Florida red tilapia
Desprez, D.; Geraz, E.; Hoarea, M. C. et al

in Aquaculture (2003), 216(1-4), 55-65

This paper reports the effects of a natural androgen, 11beta-hydroxyandrostenedione (11betaOHA4), on sex-reversed fry of the Florida red tilapia. In a first approach, the optimal dosage and duration were ... [more ▼]

This paper reports the effects of a natural androgen, 11beta-hydroxyandrostenedione (11betaOHA4), on sex-reversed fry of the Florida red tilapia. In a first approach, the optimal dosage and duration were determined in the laboratory. The sex-reversal treatment was applied on post-yolksac stage fry (10 days after fertilisation at 27degreesC. Dosages of 20, 30, 40 and 50 mg of 11betaOHA4 kg(-1) of food were used during periods ranging from 10 to 35 days. Dosages of 40 or 50 mg of 11betaOHA4 kg(-1) of food during 21-35 days significantly increased the male percentage in sex-reversed groups in comparison to control groups. A lower but still significant deviation of the male percentage was observed when a dosage of 40 mg kg-1 was given during at least 28 days. Lower dosages did not significantly affect the sex ratio of treated groups. Based on the results of these observations, a follow-up study was conducted to evaluate the efficiency of this natural androgen for an intensive production of sex-reversed fry. Optimal dosage (50 mg kg-1) and treatment duration (28 days) were applied to increasing stocking density of fry (8000-11,000 fish m(-2)), producing a mean male percentage of 99.1% on the 510,000 treated fry. This study demonstrates the masculinizing efficiency of 11betaOHA4 in Florida red tilapia. Interest of a natural androgen, used in sex-reversal treatment, is discussed, particularly for the Florida red tilapia strain. (C) 2003 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailProduction of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.
Bechet, Max; Castera-Guy, Joany; Guez, Jean-Sebastien et al

in Bioresource technology (2013), 145

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin ... [more ▼]

Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin producer. Compared to the parental strain, both mutants overproduced constitutively mycosubtilin, while BBG125 had lost the ability to synthesize surfactin. Surprisingly, BBG125 was found to produce about 2-fold less mycosubtilin than BBG116 despite an expected higher availability of the cytoplasmic precursors and cofactors pool for biosynthesis. Further physiological characterization of BBG125 also highlighted: (i) a strong influence of temperature on mycosubtilin biosynthesis in BBG125 with a maximal productivity observed at 22 degrees C, compared to 15 and 30 degrees C; (ii) substantial changes in fatty acid profiles and thereby in mycosubtilin isoforms, compared to the wild-type strain; and (iii) the presence of five novel mycosubtilin isoforms. The antifungal activities of the new mix were higher than or equal to those of purified isoforms. [less ▲]

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See detailProduction of active oxygen species by isolated human chondrocytes.
Henrotin, Yves ULg; Deby-Dupont, G.; DEBY, C. et al

in British Journal of Rheumatology (1993), 32(7), 562-7

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric ... [more ▼]

The ability of isolated human chondrocytes to produce active oxygen species has been investigated. The two methods for determining H2O2 and hydroxyl radicals (.OH) production were, by a fluorimetric method (production of dichlorofluorescein from a precursor in the presence of horseradish peroxidase and H2O2) and by a chromatographic method (measurement of ethylene production from gamma-methiol-keto-butyric acid after .OH attack). Chondrocytes were tested, both with and without activation by phorbol myristate acetate (PMA: 10(-6) M), in the presence of Ca2+ (1 x 10(-4) M) and Mg2+ (2 x 10(-4) M) or after variable periods of anoxia under nitrogen (4 to 12 h) followed by reoxygenation (with 95% O2, 5% CO2). Under these experimental conditions, the PMA-excited chondrocytes produced from 80 to 180 nmol of hydrogen peroxide per 1 x 10(6) cells and chondrocytes subjected to anoxia-reoxygenation produced up to 1700 nmol H2O2 per 1 x 10(6) cells. The hydroxyl radical production by PMA or anoxia-reoxygenation excited cells reached 600% of the production of non-excited cells and 1300% when they were subjected to successive stimulations by PMA and anoxia-reoxygenation. The possible pathological significance of these observations is discussed. The results indicate that stimulated human chondrocytes are capable of producing active oxygen species which could play a major role in joint inflammation and cartilage damage. [less ▲]

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