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See detailQuantitative and qualitative estimation of bacteria in foodstuffs by genetic amplification
China, Bernard; Ghafir, Yasmine; Daube, Georges ULg

in Annales de Médecine Vétérinaire (2002), 146(2, APR-MAY),

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed ... [more ▼]

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed until now require the growth of the microorganism on a selective medium allowing to differentiate it from other bacteria. This procedure could be long and difficult for the analysis. To improve these classical methods, molecular biology techniques based on the specific amplification of a DNA sequence are in development. Moreover, the apparition of real time PCR allows new future prospects since it allows to quantify the number of bacteria present in a sample. This quantitative aspect is very important in food microbiology. In this way, the molecular biology tools should allow to develop fast, sensitive, specific and quantitative diagnosis tests to confirm the results obtained by classical methods but also to allow the diagnosis of fastidious bacteria. [less ▲]

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See detailQuantitative and qualitative hydrogeological study of the alluvial aquifer of Somes-Szamos (RO-HU) NATO SfP Project 973684 SQUASH
Dassargues, Alain ULg; Lenart, Laszlo; Drobot, Radu

in Hidrotehnica (2004), 49(9-10), 3-7

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See detailQuantitative and qualitative modifications of pyoverdines from Pseudomonas putida BTP16 induced by culture medium variations.
Ongena, Marc ULg; Jacques, P.; Delfosse, Ph. et al

Poster (1993, August)

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See detailQuantitative and qualitative study of spelt and wheat fibers in varying milling fractions
Escarnot, Emmanuelle ULg; Agneessens, Richard; Wathelet, Bernard ULg et al

in Food Chemistry (2010), 122

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See detailQuantitative and qualitative study of the production of broad-spectrum antifungal lipopeptides from Bacillus subtilis S499.
Jacques, Ph.; Hbid, Ch.; Vanhentenryck, F. et al

Poster (1993, June)

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See detailQuantitative and qualitative study of the production of broad-spectrum antifungal lipopeptides from Bacillus subtilis S499.
Jacques, Ph.; Hbid, Ch.; Vanhentenryck, F. et al

in Progress in biotechnology (1994), 9

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See detailA quantitative approach to ecotone creation for nature reserve design.
Bogaert, Jan ULg; Salvador-Van Eysenrode, D; Van Hecke, P et al

in The European dimension in ecology, perspectives and challenges for the 21th century (1999)

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See detailQuantitative approach to the conservation of a tropical seabird population in the western Indian Ocean
Monticelli, David; Ramos, J. A.; Doucet, Jean-Louis ULg et al

Poster (2006)

The roseate tern (Sterna dougalli) is an oceanic seabird species showing mainly a tropical distribution, with a relatively fragile status worldwide. It was still recently considered as a "near-threatened ... [more ▼]

The roseate tern (Sterna dougalli) is an oceanic seabird species showing mainly a tropical distribution, with a relatively fragile status worldwide. It was still recently considered as a "near-threatened" (IUCN red list) due to the strong decline in population trends over the last decades. For instance, the annual breeding population found on Aride Island, western Indian Ocean, has fluctuated between a high of 4,000 in the 1970's to a low of 60-1200 pairs from 2000 onwards. As a result, an intensive monitoring program was conducted on Aride between 1998 and 2006 in order to understand how environmental factors may affects this breeding population. This included the estimation of annual productivity (no of chicks fledged per pair), demographic parameters such as survival rate and recruitment (age-specific breeding probabilities), and their affecting co-factors (e.g. food availability, tick parasitism,...). We suggest that these three parameters (productivity, survival, recruitment) constitute the necessary basis to model the life-cycle of this population, and, ultimately, to provide local managers with conservations measures. [less ▲]

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See detailQuantitative approaches based on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Raman Chemical Imaging (SER-CI)
De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg; Dumont, Elodie ULg et al

Conference (2014, January 20)

Surface-enhanced Raman scattering (SERS), discovered in 1978, is a recent technique enabling to circumvent the main limitations of classical Raman spectroscopy by dramatically exalting the Raman ... [more ▼]

Surface-enhanced Raman scattering (SERS), discovered in 1978, is a recent technique enabling to circumvent the main limitations of classical Raman spectroscopy by dramatically exalting the Raman scattering of the target molecules which are adsorbed or very closed to metallic surfaces while reducing the fluorescence impact on spectra [1]. This technique combines the sensitivity of the fluorescence keeping the structural information of molecules obtained from the SERS spectrum [2]. This last point allows to implement multiplex analyses. Moreover, it is possible to perform Surface-enhanced Raman chemical imaging (SER-CI) analyses which enable to acquire a visual representation of samples combining spectral and spatial measurements. Therefore SERS could become an attractive technique in numerous fields such as pharmaceutical and biomedical research. In this context, the feasibility of developing quantitative approaches using SERS and SER-CI on a pharmaceutical model was studied. The aim was to develop methods allowing the quantification of 4-aminophenol (4-AP) in a pharmaceutical formulation based on paracetamol. 4-AP is the main impurity of paracetamol and is actively research because of its toxicity. This pharmaceutical model was first investigated using SERS and a quantitative method enabling to quantify 4-AP from 3 to15 µg/mL was developed and validated using the standard addition method as a calibration method [3]. From these results, the possibility of developing a quantitative approach using SER-CI was investigated. Tablets based on paracetamol comprising different concentrations of 4-AP were prepared. Different ways to cover the sample surface by the SERS substrate were tested and a homogeneity study was performed to improve the repeatability of SER-CI analyses. Different spectral intensity normalizations were also tested in order to optimize the SER-CI method. Finally, a quantitative approach using SER-CI was developed allowing the quantification of 4-AP from 0.025% to 0.2% (w/w) in paracetamol tablets [4]. This first quantitative approach could pave the way to quantitative analysis of small molecules using SER-CI in complex matrices. References [1] P.L. Stiles, J.A. Dieringer, N.C. Shah, R.P. Van Duyne, Annu. Rev. Anal. Chem. 1 (2008) 601-626. [2] R.F. Aroca, R.A. Alvarez-Puebla, N. Pieczonka, S. Sanchez-Cortez, J.V. Garcia-Ramos, Adv. Colloid Interface Sci. 116 (2005) 45-61. [3] C. De Bleye, E. Dumont, E. Rozet, P.-Y. Sacré, P.-F. Chavez, L. Netchacovitch, G. Piel, Ph. Hubert, E. Ziemons, Talanta 116 (2013) 899-905. [4] C. De Bleye, P.-Y. Sacré, E. Dumont, L. Netchacovitch, P.-F. Chavez, G. Piel, P. Lebrun, Ph. Hubert, E. Ziemons, J. Pharm. Biomed. Anal. (in Press) [less ▲]

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See detailQuantitative aspect of serum myoglobin changes in acute myocardial infarction
Chapelle, Jean-Paul ULg; Albert, Adelin ULg; Smeets, J. P. et al

in European Heart Journal (1981), 2(A), 213

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See detailQuantitative aspects of serum myoglobin changes in acute myocardial infarction
Chapelle, Jean-Paul ULg; Albert, Adelin ULg; Smeets, J. P. et al

Poster (1981, September)

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See detailQuantitative aspects of the spatial distribution and morphological characteristics of the sea bass (Dicentrarchus labrax L.; Teleostei, Serranidae) trunk lateral line neuromasts.
Faucher, Karine ULg; Lagardère, Jean-Paul; Aubert, Anne

in Brain, Behavior & Evolution (2005), 65(4), 231-43

The results presented herein report quantitative data relative to the distribution and morphological characteristics of both types of neuromasts encountered on the trunk lateral line of the sea bass ... [more ▼]

The results presented herein report quantitative data relative to the distribution and morphological characteristics of both types of neuromasts encountered on the trunk lateral line of the sea bass (Dicentrarchus labrax, L.). These data were obtained from scanning electron micrographs. They indicate that, as expected, each modified scale of the sea bass possessed a single canal neuromast with long axis oriented parallel to the fish's long axis. In contrast to several fish species, two thirds of superficial neuromasts observed herein were oriented perpendicular to the fish's long axis. However, whatever the main orientation of superficial neuromasts, two thirds of their hair bundles were oriented parallel to the long axis of the animal with approximately half of them in the direction of the head. Similar ratios were observed for canal neuromasts whatever the area of the maculae: central or peripheral. For both types of neuromasts it was not possible to clearly distinguish a paired organization of hair bundles with opposing polarities. Superficial neuromasts on each trunk canal scale were located on either the dorsal or ventral side of the canal and appeared to be distributed along the trunk lateral line with a higher probability to be encountered closer to the operculum. The frequency of presence and the average number of superficial neuromasts per scale increased with fish size. We observed a size gradient for canal neuromasts between the operculum and caudal peduncle. This gradation was correlated with a reduction of the width of the central area of the canal segment. Canal neuromasts were always localized in the larger portions of the canal segments. Taken together, these results point out some specific features associated with the sea bass trunk lateral line. With the previous report, they establish the first full description of the trunk lateral line of sea bass and will be useful for upcoming experiments regarding the function of the two types of neuromasts. [less ▲]

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See detailQuantitative Assessment of Aerial Ammonia Toxicity to the Nasal Mucosa by Use of the Nasal Lavage Method in Pigs
Urbain, B.; Gustin, Pascal ULg; Prouvost, J. F. et al

in American Journal of Veterinary Research (1994), 55(9), 1335-1340

Effects of atmospheric ammonia (NH3) on the nasal mucosa and somatic growth were investigated in pigs exposed to 4 NH3 concentrations (0; 25; 50; and 100 ppm) for 6 days in a specifically designed air ... [more ▼]

Effects of atmospheric ammonia (NH3) on the nasal mucosa and somatic growth were investigated in pigs exposed to 4 NH3 concentrations (0; 25; 50; and 100 ppm) for 6 days in a specifically designed air-pollutant exposure chamber. Nasal lavage (NAL) was applied to quantify the ammonia-induced inflammatory response by measuring the number of neutrophils and the albumin (porcine serum albumin) concentration in the NAL liquid. In control pigs, these variables remained unchanged throughout the exposure period. In all other groups, an important ammonia concentration-related increase was recorded. The equation of the linear regression line established between the mean values of the number of neutrophils (x 10(3)) per milliliter of NAL liquid (y) recorded at the end of the exposure period and the ammonia concentrations (ppm) was: y = 69.7 + 3.3 [NH3] (r = 0.979; P < 0.020). The increase in the neutrophil count was significant (P < 0.05) at concentrations as low as 25 ppm. For albumin concentration nanograms per milliliter, the corresponding equation was: y = 574 + 14.3 [NH3] (r = 0.953; P < 0.045). However, the first significant change (P < 0.05) in this variable was only obtained for the higher concentration (100 ppm). In exposed pigs, a concentration-related depression of somatic growth was observed. The equation of the regression line plotted relating the individual values of the changes in body weight gain recorded over the exposure period expressed as percentage of the initial body weight (y) and the ammonia concentration was: y = 3.507-0.203 [NH3] + 0.001 [NH3]2 (r = 0.55; P < 0.010).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailQuantitative assessment of erythropoiesis and functional classification of anemia based on measurements of serum transferrin receptor and erythropoietin.
Beguin, Yves ULg; Clemons, G. K.; Pootrakul, P. et al

in Blood (1993), 81(4), 1067-76

We evaluated the quantitative value of a simple model of erythropoiesis, based on the basic assumptions that the red blood cell (RBC) mass determines erythropoietin (Epo) production, which in turn ... [more ▼]

We evaluated the quantitative value of a simple model of erythropoiesis, based on the basic assumptions that the red blood cell (RBC) mass determines erythropoietin (Epo) production, which in turn stimulates erythropoietic activity. The RBC mass was quantitated by direct isotopic measurement (RCM), Epo production by serum Epo levels, and erythropoiesis by the ferrokinetic measurement of the erythron transferrin uptake (ETU), the serum transferrin receptor (TfR) level, and the reticulocyte (retic) index, and was completed by an evaluation of overall marrow erythron cellularity. We studied a total of 195 subjects, including 31 normal individuals, 38 patients with polycythemia, and 126 patients with various forms of anemia. Instead of only quantitating Epo and erythropoiesis in absolute terms, we also evaluated them in relation to the degree of anemia or polycythemia, and expressed the results as a ratio of observed values to values predicted from the regression equations between hematocrit (Hct) on the one hand, and Epo, TfR, and ETU on the other, obtained in a carefully selected subpopulation. The slope of the regression of TfR (as well as ETU) versus Hct was very similar to the slope of the regression of Epo versus Hct. Average EPO and TfR (as well as ETU) values predicted from the regression equations were quite comparable to observed values in most groups of subjects, with exceptions predictable from knowledge of the pathophysiology of these hematologic disorders. We identified four major patterns of erythropoiesis, ie, normal, hyperdestruction (with variants of hemolysis or ineffective erythropoiesis), intrinsic marrow hypoproliferation, and defective Epo production. Dissecting out groups of patients showed much greater heterogeneity than when patients were analyzed by group. This was particularly true in the case of a hypoproliferative component being combined with hyperdestruction, giving what we called a "mixed disorder of erythropoiesis." We conclude that the pathophysiology of anemia can be assessed by a simple measurement of Hct, retic index, Epo, and TfR levels, with Epo and TfR being more informative when expressed in relation to the degree of anemia. The model is particularly useful for detecting the presence of multiple mechanisms of anemia in the same patient. However, it has limitations inherent to the relative invalidity of TfR in iron deficiency, the imprecision of a retic count, and the difficulty in distinguishing hemolysis from ineffective erythropoiesis in some patients and in recognizing a component of hyperdestruction in hypoproliferative anemia. [less ▲]

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See detailQuantitative assessment of erythropoiesis in haemodialysis patients demonstrates gradual expansion of erythroblasts during constant treatment with recombinant human erythropoietin.
Beguin, Yves ULg; Loo, Martine; R'Zik, Samir ULg et al

in British Journal of Haematology (1995), 89(1), 17-23

Recombinant human erythropoietin (rHuEpo) has been shown to be effective in correcting the anaemia of chronic renal failure. It has been reported that reticulocytes as well as erythroid progenitors ... [more ▼]

Recombinant human erythropoietin (rHuEpo) has been shown to be effective in correcting the anaemia of chronic renal failure. It has been reported that reticulocytes as well as erythroid progenitors increase within 1-2 weeks, with no further elevation beyond this time interval. However, the erythroblast pool is quantitatively the most important compartment of erythropoiesis, and the rate, extent and duration of the expansion of erythropoietic activity in response to rHuEpo is not known. Treatment with rHuEpo was given to 64 patients i.v. thrice weekly after haemodialysis. The effect of rHuEpo was obvious from the early elevation of reticulocyte counts, but much of this increase was due to a rapid output of shift reticulocytes which levelled off after a few weeks. Serum transferrin receptor (TfR), a quantitative measure of erythropoiesis, increased progressively over 6 weeks to reach a plateau phase at about twice baseline values. The Hct increased progressively and continued to rise steadily after the TfR plateau was reached. The speed and extent of the expansion of erythropoietic activity correlated with the later haematological response to rHuEpo. When rHuEpo was discontinued, erythropoietic activity returned progressively to baseline values, to rise again gradually when treatment was resumed. Part of the Hct increase was also due to haemoconcentration. The results indicate that changes in the various erythroid compartments vary considerably in intensity and speed, and that the erythroblast compartment in particular is slow to respond to modifications in the erythropoietin stimulus. [less ▲]

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See detailQuantitative assessment of global and regional left ventricular function with low-contrast dose digital subtraction ventriculography.
Mancini, G. B.; Hodgson, J. M.; Legrand, Victor ULg et al

in Chest (1985), 87(5), 598-602

Few studies have compared the use of low-contrast dose digital subtraction ventriculography with conventional ventriculography for quantitative assessment of both global and regional left ventricular ... [more ▼]

Few studies have compared the use of low-contrast dose digital subtraction ventriculography with conventional ventriculography for quantitative assessment of both global and regional left ventricular function. Accordingly, 34 patients underwent conventional ventriculography using 36 ml of ionic contrast material and digital ventriculography (mask-mode) using 10 ml of contrast diluted in 10 ml of saline and injected over two seconds. Data from two patients were excluded because of ectopy during cineventriculography and from one because of ectopy during both studies. End-diastolic and end-systolic volumes were calculated from both studies by an area-length method and used to calculate ejection fractions. Regional wall motion was quantitated by the centerline method. Results of linear regression analysis demonstrated high correlations for all parameters (end-diastolic volume, r = 0.85; end-systolic volume, r = 0.93; ejection fraction, r = 0.92; quantitative regional wall motion, r = 0.90). Thus, low-contrast dose digital subtraction ventriculography provides an accurate assessment of both global and regional ventricular function and minimizes the required dose and inherent risks of contrast media. [less ▲]

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See detailQuantitative assessment of myocardial contractile reserve during exercise in patients with mitral valve prolapse
Lancellotti, Patrizio ULg; Cosyns, B.; Van Camp, G. et al

in Circulation (2006, October 31), 114(18, Suppl. S), 707-708

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